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<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQE5</accession>
    <name>E3JQE5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 9">Glycylpeptide N-tetradecanoyltransferase</fullName>
            <ecNumber evidence="4 9">2.3.1.97</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_00180</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Adds a myristoyl group to the N-terminal glycine residue of certain cellular proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 9">
            <text>N-terminal glycyl-[protein] + tetradecanoyl-CoA = N-tetradecanoylglycyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15521"/>
            <dbReference type="Rhea" id="RHEA-COMP:12666"/>
            <dbReference type="Rhea" id="RHEA-COMP:12667"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57385"/>
            <dbReference type="ChEBI" id="CHEBI:64723"/>
            <dbReference type="ChEBI" id="CHEBI:133050"/>
            <dbReference type="EC" id="2.3.1.97"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the NMT family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.97" evidence="4 9"/>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307230.1">
        <property type="nucleotide sequence ID" value="XM_003307182.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQE5"/>
    <dbReference type="FunCoup" id="E3JQE5">
        <property type="interactions" value="681"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JQE5"/>
    <dbReference type="GeneID" id="10528219"/>
    <dbReference type="KEGG" id="pgr:PGTG_00180"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00180"/>
    <dbReference type="eggNOG" id="KOG2779">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022882_2_0_1"/>
    <dbReference type="InParanoid" id="E3JQE5"/>
    <dbReference type="OMA" id="GWKRDWH"/>
    <dbReference type="OrthoDB" id="60315at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004379">
        <property type="term" value="F:glycylpeptide N-tetradecanoyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000042">
        <property type="entry name" value="Glycylpeptide N-tetradecanoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000056">
        <property type="entry name" value="Glycylpeptide N-tetradecanoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000903">
        <property type="entry name" value="NMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022677">
        <property type="entry name" value="NMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022678">
        <property type="entry name" value="NMT_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022676">
        <property type="entry name" value="NMT_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11377:SF5">
        <property type="entry name" value="GLYCYLPEPTIDE N-TETRADECANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11377">
        <property type="entry name" value="N-MYRISTOYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01233">
        <property type="entry name" value="NMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02799">
        <property type="entry name" value="NMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00975">
        <property type="entry name" value="NMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycylpeptide N-tetradecanoyltransferase N-terminal" evidence="12">
        <location>
            <begin position="192"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Glycylpeptide N-tetradecanoyltransferase C-terminal" evidence="13">
        <location>
            <begin position="366"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="24"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="53"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="183"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="193"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EFP74224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="638" mass="70589" checksum="50CBFAD0F677D1FE" modified="2011-01-11" version="1">MSSEPEDQGVIEYGSEVLRPCGDSESNQAEAQGAQSASSRAGPSSNYSPPIGPEDEEDQEEGQDVGLPGGSAGPSTKKKKKKSKGKALSKNPSEVPADDGSLVEDHTTSEAHKMIIEQIRAQNGDEVADRISALGPKELGKMMDQIRLLEMMDGKSGGTHKNKKDMADHKFWATQPVRRLDEQDSSFEEGEIEPSKSEEEIRKSPLALPDGYEWCTLDLGDESQLRELYELLTVNYVEDDDAMFRFDYTAPFIEWALRPPGFVQDWHAGVRVASGKRRLIAFIAGIPMNLQVRSTRKPCAEINFLCIHKKLRSKRLAPVLIKEITRRCNMQGVFQAIYTAGIFLPTPISRCQYFHRNLNPAKLIATGFSPQPRGVSVARLKRMYDCPAQPQIPGFREIEEKDVDAVAVLLRLYMSRFDLIPVMSDEEVRHNFISGKGTGPVVNGRRNQQVTWTFVVENPTTHLITDLVSFYHLPSTAMKCTPHQTIDAAYLFYYATTAAPSCAHLGQPAQVQEEPSQVAVTEPTEQPNWSAEAPEARQILKKRLISLIGDALTMAQQANFDVFNALTLMDNSLFVKELQFGAGDGYLHYYLYNWKVHQIRGGVPGEPGSEEDAAQATSSGKQLIEAPGVGSGIGVVML</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQQ4</accession>
    <name>E3JQQ4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_00360</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307410.1">
        <property type="nucleotide sequence ID" value="XM_003307362.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQQ4"/>
    <dbReference type="GeneID" id="10546788"/>
    <dbReference type="KEGG" id="pgr:PGTG_00360"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00360"/>
    <dbReference type="HOGENOM" id="CLU_1326957_0_0_1"/>
    <dbReference type="InParanoid" id="E3JQQ4"/>
    <dbReference type="OMA" id="WTSHSIF"/>
    <dbReference type="OrthoDB" id="2506399at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="42"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="130"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP74404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="207" mass="23391" checksum="7AE50CD8939AF62D" modified="2011-01-11" version="1">MAEQILLGFFWCSLASLALVAWTSQSNFFNSILSIILEFLSIFKIFGIALLAIISLGIVIVLWEQVISKTQLWNKISSTYLKTSNNHAQTSSSQDGPQDEQRSILPIVIVIVGLGIFAILDTSFLLPSGQQNSSSKHSQHSQPPRNKKSNQNKTVIQFFSAILTLAVVVGFIVFNKDEYRAWKARRRLSKHHRRRARQEHSLNSPLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3JSH6</accession>
    <name>E3JSH6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01542</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP74949.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319368.2">
        <property type="nucleotide sequence ID" value="XM_003319320.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JSH6"/>
    <dbReference type="GeneID" id="10547231"/>
    <dbReference type="KEGG" id="pgr:PGTG_01542"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01542"/>
    <dbReference type="HOGENOM" id="CLU_022557_0_0_1"/>
    <dbReference type="InParanoid" id="E3JSH6"/>
    <dbReference type="OrthoDB" id="2503217at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <begin position="210"/>
            <end position="226"/>
        </location>
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        </location>
    </feature>
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            <begin position="550"/>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="690"/>
            <end position="700"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP74949.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="732" mass="83442" checksum="E3598A192A6D7E5C" modified="2012-04-18" version="2">MTRPSAACLDRLENQADMERVKRLSSQFDQFFIQASKHAIELKAYQKLIAESEKHARSDPNSQHWLNQIEEKSARQSQVQHAINQLVTQAVQQIDQHIRTEAISIIRALHSDGFFDKPGKNSNLNSNLSPTALKTIEKSIDNERKEREDSLATFKKEFEDYRTFNNTRFEELQASFQEFKESNQTRFKNLEDTHRQQMCSLRASLKLTNQQQTSSSPQPPVEQAEPQQLKQLIQEQVQEHLHTKHIPPTDLISKQSLQTAIKLIKEQLDNLGLQITETLKEFEPQIKQITQAIQLQVIKSTKTVISDGHKDIATLINGSIVKLQDQVDKLEPLINSSSERIDRIEADHLNLDSQINEKVHSKLESQLNEKVHPQIESQINQNLQSNLEDQINKKLDPKLAPLKKQLADLTQKQHEHDKKLNYPKALQSGMSKLETRQTNSYEKIGKLEKTITDVQSQFKDFELRIKPLQDRIEGVETTVQNSNHDHHNPSRRPSTPAYQANQPSHSHRRTSQTPPSPHSHSSSSNVNGRFPPSEIYNPTPTQSRRQSYQANRASTTSSSALAAQSQNISIASRLTNGPAQGGPVASSQTNGETTYYRKRTLSRESSISRPSQSNVAGSSHWPASTTNVGSGQSHNYATQPPSYEPQSDQSYSQFAHQQSQQPVSSIPLAKRLKNDYSLVNSSANHSSYHNNHDHQSEYHQFHTHSYQQQQPYHHHHPTPNPYKKNFTYTKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3JSJ4</accession>
    <name>E3JSJ4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01560</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP74967.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319386.2">
        <property type="nucleotide sequence ID" value="XM_003319338.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JSJ4"/>
    <dbReference type="GeneID" id="10547249"/>
    <dbReference type="KEGG" id="pgr:PGTG_01560"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01560"/>
    <dbReference type="eggNOG" id="KOG1577">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1687547_0_0_1"/>
    <dbReference type="InParanoid" id="E3JSJ4"/>
    <dbReference type="OrthoDB" id="2498080at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="2,5-DIKETO-D-GLUCONIC ACID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827:SF13">
        <property type="entry name" value="ALDO_KETO REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="1">
        <location>
            <begin position="45"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP74967.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="156" mass="16879" checksum="F68927B8C442851F" modified="2012-04-18" version="2">MSSFGLAASEGSAFNPSMNFADTLTIESRITLNSGHQIPCMGFGTSSLRNAEEACNEAIKIGYRHLDSAQSYGTEAAVAQAAQKCESGWESIFLTTKIPGTKHGKEACEEALRESLVHTANKPWDLVLLHEPLSEPNKRHQAYAVLAEAQKKGDVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3JST5</accession>
    <name>E3JST5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01703</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75110.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319529.2">
        <property type="nucleotide sequence ID" value="XM_003319481.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JST5"/>
    <dbReference type="GeneID" id="10547552"/>
    <dbReference type="KEGG" id="pgr:PGTG_01703"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01703"/>
    <dbReference type="HOGENOM" id="CLU_146865_0_0_1"/>
    <dbReference type="InParanoid" id="E3JST5"/>
    <dbReference type="OrthoDB" id="2500295at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="59"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75110.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="113" mass="13001" checksum="938D69AAB4C7EE6F" modified="2012-04-18" version="2">MLFLGSNYRLLPTPLEPSVAQPIAVKSIIQRNLYRHAKVELGIYLLLDSEPAESNDNLSSLSSTGHQSSSSTEDYWNDCSETSISFDEFIPAHLYTPRRLPITQLIKNWWRYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3JSY7</accession>
    <name>E3JSY7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01755</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75162.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319581.2">
        <property type="nucleotide sequence ID" value="XM_003319533.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JSY7"/>
    <dbReference type="GeneID" id="10547604"/>
    <dbReference type="KEGG" id="pgr:PGTG_01755"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01755"/>
    <dbReference type="HOGENOM" id="CLU_1190363_0_0_1"/>
    <dbReference type="InParanoid" id="E3JSY7"/>
    <dbReference type="OrthoDB" id="2515832at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75162.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="238" mass="26279" checksum="416350FE1C1A2DBF" modified="2012-04-18" version="2">MLPNLMKLGRVDETLAAECTANPAQTHLNSRSRPNYKVHHPAPPPLAGRPFPPKTTYITTIAQSLSFPFASTSNTNHIITEGTSIHPSHKDLFIHRVSQAKEEITMTSIAELNKNGGQAGSAGRQDVVLELEPQVPHQEPPHPPPVYSLTKSSDYQVPQPQDTQRSFDSVDLEAQTVPHRHPHSHQAPAGLDEPEQNPDEKRSSLRLRGGCCCCDCLYDLLRCLLCCCIFEAICDMCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>E3JT37</accession>
    <name>E3JT37_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mannosyltransferase</fullName>
            <ecNumber evidence="10">2.4.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_01857</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Mannosyltransferase involved in glycosylphosphatidylinositol-anchor biosynthesis. Transfers the third mannose to Man2-GlcN-acyl-PI during GPI precursor assembly.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 22 family. PIGB subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.-" evidence="10"/>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75264.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319683.2">
        <property type="nucleotide sequence ID" value="XM_003319635.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JT37"/>
    <dbReference type="FunCoup" id="E3JT37">
        <property type="interactions" value="544"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JT37"/>
    <dbReference type="GeneID" id="10542638"/>
    <dbReference type="KEGG" id="pgr:PGTG_01857"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01857"/>
    <dbReference type="eggNOG" id="KOG1771">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012353_0_1_1"/>
    <dbReference type="InParanoid" id="E3JT37"/>
    <dbReference type="OrthoDB" id="416834at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000026">
        <property type="term" value="F:alpha-1,2-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005599">
        <property type="entry name" value="GPI_mannosylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22760:SF4">
        <property type="entry name" value="GPI MANNOSYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03901">
        <property type="entry name" value="Glyco_transf_22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3 10" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="240"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="281"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="331"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="446"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="24"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="531"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="38"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="531"/>
            <end position="548"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP75264.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="665" mass="75238" checksum="12FECBFC8365F846" modified="2012-04-18" version="2">MQPCKESQSQPTLKNRIFNLLSRSEPRVKRATHSSLPTKRDRTERRVSSTSTNQNTSKPDEQRQPKMAAITLRLIGAIYLFRLLNALLCRTYFQPDEYYQSLEIAHRLVFGYGYQSWEWRPLPDGSTGGIRSPLHPFLFVPLYWLLRILRLDHTRLIIILPKVLQAGIATVTDLATYSLASVLLTPSYAAAALACSLTSLFNAYAGIRTFSNSLETALTASALAYWPWNPAQISSSWANLSFSILLIALSVIIRPSSILFWSLLGYTLLQKSNGETRMDVMAIVAVMGVISALACFLIDSSFYGVATFTPLNFVRQNLLNDISSFYGVNRLHFYFTQAFTFVNFSMFPTVILGMNMLATPQNDHESRGQLDRLRSARVAVLGTMLGLSVLRHKEFRFIEPLLPLFNCFAARAMVNNYIKDRLARPATSPADVSPLERILSARPLGFILRSSISLLAAVYLLGFHYRAQVSVIDYLREVPDLQLKSVGFLTPCHSTPWQSHLHKPHLAPNGTQERLWMLNCEPPLVSFQNSSLSEADSTANTTTYQNATESDEDSERKRETIATTKQMNETREYEDEASEFYGDPTGFLRARFPGTVDRGYPATDKSLQSYQWPSHLVLFEALWNHTSVRELLQTLGYQVVWNQTNGWWHDDPQRRAGSVIVLRSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3JT75</accession>
    <name>E3JT75_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_01947</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319773.1">
        <property type="nucleotide sequence ID" value="XM_003319725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JT75"/>
    <dbReference type="GeneID" id="10542901"/>
    <dbReference type="KEGG" id="pgr:PGTG_01947"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01947"/>
    <dbReference type="HOGENOM" id="CLU_1797419_0_0_1"/>
    <dbReference type="InParanoid" id="E3JT75"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP75354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="144" mass="14521" checksum="DA0E1C57F435C9DA" modified="2011-01-11" version="1">MVAGGGSGLEAKVGANECPVAIEAKFIIGGVPGFIADIAPEVANEAAVFIADIAPEVVAGLDPEVFANRAPVVTDESTKLNEFNDGTVPVVIEVEARAGAGANGNPVAIVDEAPVLPPWNKVSIDWSCISISGGTTTPLIGMLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3JTA8</accession>
    <name>E3JTA8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_01980</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319806.1">
        <property type="nucleotide sequence ID" value="XM_003319758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JTA8"/>
    <dbReference type="GeneID" id="10542934"/>
    <dbReference type="KEGG" id="pgr:PGTG_01980"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01980"/>
    <dbReference type="HOGENOM" id="CLU_394370_0_0_1"/>
    <dbReference type="InParanoid" id="E3JTA8"/>
    <dbReference type="OMA" id="WLPTTYP"/>
    <dbReference type="OrthoDB" id="10318488at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP75387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="699" mass="78332" checksum="41D8DA9EA37817CA" modified="2011-01-11" version="1">MEGTAVMRNIILEARNGMGFCTLSTGIWRSIGWTSSEFDQGTQGWRSKMMTLMVALLKARSKVPPNLEVTPNPPRHPNRQPNHSWITLSNSGILTYLPKATTICQSNDLATFFLICTLSHPTCPTQSLLDYSVQLWHPDISSKGYYHLPIIGPGNLLPDLYFKPSHLPTQSLLDYSVQLWHPDISSKGYYHLPIIGPGNHPTCPTQSLLDYSVQLWHPDISSKGYYHLPIIGPGNLLPDLYFKPSHLPTQSLLDYSVQLWHPDISSKGYYHLPIIGPGNHPTCPTQSLLDYSVQLWHPDISSKGYYHLPIIGPGNHPTCPTQSLLDYSVQLWHPDISSKGYYHLLIIGPGNHPTCPTQSLLDYSVQLWHPDISSKGYYHLPIIGPGNLLPDLYFKPSHLPTQSLLDYSVQLWHPDISSKGYYHLPIIGPGNHPTCPTQSLLDYSVQLWHPDISSKGYYHLPIIGPGNLLPDLYFKPSHLPTQSLLDYSVQLWHPDISSKGYYHLPIIGPGNHPTCPTQSLLDYSVQLWHPEISSKGYYHLPIIGPGNHPTCPTQSLLDYSVQLWHPEISSKGYFHLPIIGPGKHPTCPTQSLLNYPSNSGILKYLPKATTICQSKDLATFFLICTLSHPTCTTQSLLDYSVQLWHPELSSKGYYHLPIIGPGNHPTCPTQSLLDYSVQLWHPEISSKGYYHLPIIGPGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3JTY7</accession>
    <name>E3JTY7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00839</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319927.1">
        <property type="nucleotide sequence ID" value="XM_003319879.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JTY7"/>
    <dbReference type="GeneID" id="10543224"/>
    <dbReference type="KEGG" id="pgr:PGTG_00839"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00839"/>
    <dbReference type="eggNOG" id="ENOG502SP2I">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020173_0_0_1"/>
    <dbReference type="InParanoid" id="E3JTY7"/>
    <dbReference type="OMA" id="GSNNICY"/>
    <dbReference type="OrthoDB" id="2501916at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053110">
        <property type="entry name" value="Ribosomal_L1-TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48162:SF1">
        <property type="entry name" value="RIBOSOMAL L1 DOMAIN-CONTAINING PROTEIN CG13096"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48162">
        <property type="entry name" value="YALI0A06930P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="322"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="512"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="515"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="546"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="596"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="629" mass="70796" checksum="1E8F324602868412" modified="2011-01-11" version="1">MNPWAPQNSASTLTAEQQQLVDAAVRARTSHVEDLVTQLTEQVNNLKSSANTASSQPQNQRKPAKKASDKQPAVKIKVRMSDQSSSNHKNSSAAPPKAPANLTPQRPARAASVPLEVGKPKASTSKKSKAVKQPSPKRHPQQMQTTDVPLEFASTKNALFVHIKILWGLLKQDSVPQAPELRNLQEFYNRFSNGQEVEQAAQASTSPALINTDEVQLFKDATTGSIKYGRSVIHLGSNNVRYAQGLMVRLGLRTWCPNLDEDSASLYNAAHRIAAITTFQELVAGQAYKYMNVNAKMASQTSYLIQAYNHFVHFIQHTKYHKEKKQEGKNAQDASNKRVSKNRERLRDARKEFAILNKFPPRYVHILSQIGAHSDDEHDEKANIYKIKTLPYRSKNASKFIRALDIVMKNAAEQDPSSYRKRRVRKLPRQPVMSTFTAAPKGLPIDFYDPSWYHKLVPAQQKTIPNTQAVAFLPDASQSLLPKKQRHPDEKLTDSSFTRKYWDIVVEPYGLLGDESSDESDDEEEAQGMQSAAANDSEGEGHNLDDESPDESPDEYFEEGDAGDLYDDDIEDDEEGDDEEEYNINDEDDEEDEQYSDTHENTDDVHMKDRSHGVDARTLAIMEEEEDGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3JU57</accession>
    <name>E3JU57_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NF-kappa-B-activating protein C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_00913</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NKAP family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320001.1">
        <property type="nucleotide sequence ID" value="XM_003319953.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JU57"/>
    <dbReference type="STRING" id="418459.E3JU57"/>
    <dbReference type="GeneID" id="10543298"/>
    <dbReference type="KEGG" id="pgr:PGTG_00913"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00913"/>
    <dbReference type="eggNOG" id="KOG2812">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032439_5_0_1"/>
    <dbReference type="InParanoid" id="E3JU57"/>
    <dbReference type="OMA" id="XETSREL"/>
    <dbReference type="OrthoDB" id="273141at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040466">
        <property type="entry name" value="NKAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009269">
        <property type="entry name" value="NKAP_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13087">
        <property type="entry name" value="NF-KAPPA B ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13087:SF0">
        <property type="entry name" value="NFKB ACTIVATING PROTEIN LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06047">
        <property type="entry name" value="Nkap_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NF-kappa-B-activating protein C-terminal" evidence="3">
        <location>
            <begin position="292"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="264"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="78"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="92"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="138"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="171"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP75582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="398" mass="46018" checksum="02F81B51EBBF8F1A" modified="2011-01-11" version="1">MPHHRPRASDFDPPITKRSSSPPPTTSSSHHHHHHHQRRPSPNYERGNQQRLTPEESLDDRHHHHNRNHNYQQDEHPNNNNNNSYSNGYRQGGRDDFFEKRRLDRQNAPVIGLWPKSPTQAYGEEKKKKKSKKEDRHRSSKDKKKKKSSKSKHYRSDDESDESDRSDDHLHHSRKKHKSHKKKKYDSESESSDEEARRSKKRKGKSRSVELEERRDEEEDVWMEKEVAPPPTSIVKAEAPRKESLTGPSRPVAIVEQAQALREESLDDDETDMGEVGPMPYNHATGKAMDPKEFGRALRPGEGSAMAAFIEAGERIPRRGEIGLTGTEIEKFENVGYVMSGSRHRRMNAVRIRKENQVISAEEKRGILKMQAEEKAKREGQIISSFRELVDTKLQETG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3JU67</accession>
    <name>E3JU67_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">glucan 1,3-beta-glucosidase</fullName>
            <ecNumber evidence="9">3.2.1.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_00923</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1-&gt;3)-beta-D-glucans, releasing alpha-glucose.</text>
            <dbReference type="EC" id="3.2.1.58"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.58" evidence="9"/>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75592.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320011.2">
        <property type="nucleotide sequence ID" value="XM_003319963.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JU67"/>
    <dbReference type="STRING" id="418459.E3JU67"/>
    <dbReference type="GeneID" id="10543392"/>
    <dbReference type="KEGG" id="pgr:PGTG_00923"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00923"/>
    <dbReference type="eggNOG" id="ENOG502QWCD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014563_1_0_1"/>
    <dbReference type="InParanoid" id="E3JU67"/>
    <dbReference type="OrthoDB" id="62120at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004338">
        <property type="term" value="F:glucan exo-1,3-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000303">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050386">
        <property type="entry name" value="Glycosyl_hydrolase_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297:SF1">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE I_II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297">
        <property type="entry name" value="GLUCAN ENDO-1,6-BETA-GLUCOSIDASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="6 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="12">
        <location>
            <begin position="116"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00036824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP75592.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="501" mass="55795" checksum="C00DC903785F04A7" modified="2012-04-18" version="2">MAMKRRPWAFIIIGSILAICAVVIPVAVVFTSKSESHPDPATKAASAASKAHKIGPNDLAPKPKWDFDVNKIVGINLGNWLVLERWMVEDWFVEKAGANSWDEWSFTIAQGDKAAQTLEEHWSTWVTEDDVEKLYQAGINTMRIPLGFWIFIETVPPEPYISTTQLDHLERLCGWAYARDMYIILDLHGLPGSQNGEQQSGHNTTSPNFFQPLQQARSDQLVKAVVDWIGSSAYSSIISAIEVVNEPRPYTTEQRAMLRAFYDRSYETIQTLGSKAPAMFFADGFVPGDKFAYWWEFASSHKTQPPTLIYTDHPYIGYFPAQTNAADIYNQICTKGTKYANFPVTTVITEWSLRTGIQNTTFERSFYEAQLNTWAWYSGAVFWSLRVLDSKVAVLADKVAQYQWSFESLLERGSIASPTKKGDSAQFLKELRTPCGAPPTLVRDGAPPQGTAAAAAIADAEAAKAMIPAITQSIEAAAKQFGLQGIGAKAVHKNPPKHRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3JVC7</accession>
    <name>E3JVC7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01333</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP76002.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320421.2">
        <property type="nucleotide sequence ID" value="XM_003320373.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JVC7"/>
    <dbReference type="GeneID" id="10533244"/>
    <dbReference type="KEGG" id="pgr:PGTG_01333"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01333"/>
    <dbReference type="HOGENOM" id="CLU_023255_0_0_1"/>
    <dbReference type="InParanoid" id="E3JVC7"/>
    <dbReference type="OrthoDB" id="2507275at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="436"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="554"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="274"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="303"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="440"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76002.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="723" mass="81314" checksum="66A8E679DC625ECE" modified="2012-04-18" version="2">MQSHQYPSGSPPPQTQHQTSHDPRYRTHQNVHDPRYPPPNQQHLFDAYLSQDLPYEEVQGLYSTYNIQPVDLNYHQDSLQYGDTPRYADTARYGDTLRDADMEHDLARDGLGHQDPQLLQNQPHTPRYADMERGLARDGLGHQDPQLLQNQPHTPRYADMERGLARDGLGHQGPQLSQNQPHQPSPLLANINRHNTRASQNSQNIHVLKLAPLTSVPIVPNPAHSTARTHSMGSTASHIHETPVTSADLSRLPATQRESDRSNATSIHETQDTSSRSSGVPDSRSIVPGDVRRAPDPIPARSPSPAISLINPSPTPAATTKLTPADVMEDFKKKTLSELRDLQHTHIRYKKLNLAIKIEAQNLFFDYQRKQHLLSLKYSRPFKLLTKYLGQRRTQQKQSNWHSFQKTNPAAKEALKNTNNNIGQRNKDVSKLYKQHAGTQAALPPQANPNADKVNDPNAEERERFGKIFKSDLTLRNEVKKWGQGVQLKLKELSDSFGVEGFLVLATQDHRKPLFFQGGSFLGDDYLRALLEDGNPMRKFAMWTAGSKATSKKRKSSALAASGSNETAENVDDHPSKKKTKYTVGAFENRDFCQGGLALNHTYISKQLGLLFKAAQSKNSLVDKRSRWPGTETVLRLARIDHKLVVHSNPVGLSVEHLVDVPVKKMSIEDTWLVLRGLKNKWIELVEHKFDSLPKSVAVRKRNMAAGTTAITREEDDTECQPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWV2</accession>
    <name>E3JWV2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_02968</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76527.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320946.2">
        <property type="nucleotide sequence ID" value="XM_003320898.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JWV2"/>
    <dbReference type="FunCoup" id="E3JWV2">
        <property type="interactions" value="254"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JWV2"/>
    <dbReference type="GeneID" id="10528300"/>
    <dbReference type="KEGG" id="pgr:PGTG_02968"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02968"/>
    <dbReference type="eggNOG" id="KOG1305">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009020_1_1_1"/>
    <dbReference type="InParanoid" id="E3JWV2"/>
    <dbReference type="OrthoDB" id="438545at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061459">
        <property type="term" value="F:L-arginine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005313">
        <property type="term" value="F:L-glutamate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005290">
        <property type="term" value="F:L-histidine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015189">
        <property type="term" value="F:L-lysine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015194">
        <property type="term" value="F:L-serine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005302">
        <property type="term" value="F:L-tyrosine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003333">
        <property type="term" value="P:amino acid transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF678">
        <property type="entry name" value="VACUOLAR AMINO ACID TRANSPORTER 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="148"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="212"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="251"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="294"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="374"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="399"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="489"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="11">
        <location>
            <begin position="11"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="329"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="348"/>
            <end position="360"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP76527.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="514" mass="56578" checksum="CCDBE8A315C526F3" modified="2012-04-18" version="2">MDHFNQPTIGTASFLSCVANLSNTILGTGMLAMAHGFAQGGLIIGFITVVICGSMAYLGLYLLSLCATHPSIPHRTASFSAISKFTYPQASTLFDLAIAIKCFGVSISYLLIFGKLMPQVILSFQSTITLALIDDNTETTLWLLDRRLWITLSMLVLVPVSFLPTLDSLRHISYVALIGVFNLIIVVIFKFFFSSDPLPSKSSGAEIHWIKLDPSFLTALPLFVFAYTCAQNIFSVHNEMVDNSPRQMKNVLLTCIGSAVGTYEIVGVLGYLTFGSKVSSNLISDYESSVFISISRLTISILVLFSYPLQLFPCRSSLIKVLNYQRNQDRPPKLPHDPLPTTSQALEREEDIEDSGEVEEAGVHSTDEPISIKLYVGITAFLLLGSFLIAVNVERLETVLGFVGSTGSTTISYILPGLFFRKLFPAGRSGLPSSPDLASNPELDRYYDHNQTTEEGEQQGAEELANILRERQQVEDLIDPHELVVLRKLSLALVVAGFVIMIVCFSLNIHDAFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWX7</accession>
    <name>E3JWX7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_02013</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76552.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320971.2">
        <property type="nucleotide sequence ID" value="XM_003320923.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JWX7"/>
    <dbReference type="FunCoup" id="E3JWX7">
        <property type="interactions" value="895"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JWX7"/>
    <dbReference type="GeneID" id="10528403"/>
    <dbReference type="KEGG" id="pgr:PGTG_02013"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02013"/>
    <dbReference type="eggNOG" id="KOG1128">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004905_0_1_1"/>
    <dbReference type="InParanoid" id="E3JWX7"/>
    <dbReference type="OrthoDB" id="1936594at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044244">
        <property type="entry name" value="TTC27/Emw1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16193">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16193:SF0">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="547"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="581"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="633"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="662"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP76552.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="917" mass="104285" checksum="043B9AEAA8E45251" modified="2012-04-18" version="2">MNQLEYTLIDSNQRTTTSTTNQATQLIDHFLNHRWNSIINHNLLRTYFSLDQHYQLTLTNNTHQKEEEEEEPLIKLSFIATLLSVYVQINYTGPTQFELQPISILNQTENQDQDQEESELNSISLEALSLAGEPAYHLAKSATYLLMAIQLIDKLISTVDLTQFPTIVWWKLRAEILRRQLFDQPIPLSDQLLKQIDQLSSHLSSTNHSSAYDLDLLPLLTLERGLAEHLTGNEKAANILFRKAVTQAKLEYELTGIPGKRTKFQEKEISQLVVLAQSRTRNSTITATSVPTPSTKDSMPVTLALDDDTLMEKTQFTQSTDSPLKLDPSNQPNLHPLDQALLLALSLSIKNTSPAHGLTNAQMAPFVERVLENPNNWSIHSMALLIRSRLEAHRTRTVERGLLQLQALVDQLSKEAENDPVDKATALERVRCTWSLALPSRWELEKELGQKFSGLGLTRSAMEIFERLEMWEHVVQCHLNLDSKTLGIELVRELIEGKKVESSLKMSRYRSTRDPKRLGKLWCLLAELESQPEHWQTAWNVSQHTSSRAMRSLGAHHFMQGDYPKAKECLLRALEINPLFNKTWFIYGCAAMRTDDWDEAERAFRRCVGLDDEDAEAWNNLATVHLKKASLIDESQANEGEETDVKIKPLLSDDDDDGDLLGTKDDESHDLPDEDPQPGLGRKFSRPMAAFYALQQAVKLSYDSWRMYTNYMLIAMSVGEYMEATRALGRVVEIRGEAGVDMEVLAKLVKVIMLQRSDAAKTDEWSETDKGIRQARLFERLVDVIDRVVLPKTPTMPALWELRSELAELDGDLEAALRFQMESYRVGVSATTDRWLSSLDEWLAAIELLKRTIQKLARLGPLLPTVISDENPKKSGFVNWKWQAKNLASNFLAKSKSTFDDHPSFEDLADFSKSLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3JX57</accession>
    <name>E3JX57_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_02093</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321051.1">
        <property type="nucleotide sequence ID" value="XM_003321003.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JX57"/>
    <dbReference type="GeneID" id="10528569"/>
    <dbReference type="KEGG" id="pgr:PGTG_02093"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02093"/>
    <dbReference type="HOGENOM" id="CLU_665865_0_0_1"/>
    <dbReference type="InParanoid" id="E3JX57"/>
    <dbReference type="OrthoDB" id="2508171at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP76632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="406" mass="47086" checksum="2AC71B4E44574813" modified="2011-01-11" version="1">MLFREEFQSLARLVGIFEEPDQPQTCQDRLVQALKTLGRKSKRDHKRALKRSLRREANYIDRSKLRNNVRWMFKTKHLPLLHEQIKQLSVCLDPTSMPLDPNPRFQAGLTVLSEINDSVDQIVASISRIWESPKPEDSQENVEDLTVLRCHRMGSKYLNNVMIGLSGILESYRGCFAVFTIREDGTVNIGNSYRTPLIPSDVDTELCGIKRFIEWLGRSNISVLQDEWHSIIIEYESLLCDIMIFVNSPEVEDDPEELMLREQAEGLIPLVKLSRLFINKLRLTNSQPRLTSHMDFGHLEALRCVAETMSSELYVIFDALYESRPHTKKLIDAMIALDAAFKDITAIFIDHFDSLDPNSDPEAYQSHRRWIQLWLSQFNVAIENFIWLSHDHIIEDITTGSEYEDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXA4</accession>
    <name>E3JXA4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">2-oxoglutarate dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="5">1.2.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">2-oxoglutarate dehydrogenase complex component E1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PGTG_02140</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>N(6)-[(R)-lipoyl]-L-lysyl-[protein] + 2-oxoglutarate + H(+) = N(6)-[(R)-S(8)-succinyldihydrolipoyl]-L-lysyl-[protein] + CO2</text>
            <dbReference type="Rhea" id="RHEA:12188"/>
            <dbReference type="Rhea" id="RHEA-COMP:10474"/>
            <dbReference type="Rhea" id="RHEA-COMP:20092"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:83099"/>
            <dbReference type="ChEBI" id="CHEBI:83120"/>
            <dbReference type="EC" id="1.2.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the alpha-ketoglutarate dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.4.2" evidence="5"/>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76679.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321098.2">
        <property type="nucleotide sequence ID" value="XM_003321050.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXA4"/>
    <dbReference type="FunCoup" id="E3JXA4">
        <property type="interactions" value="207"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JXA4"/>
    <dbReference type="GeneID" id="10528618"/>
    <dbReference type="KEGG" id="pgr:PGTG_02140"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02140"/>
    <dbReference type="eggNOG" id="KOG0450">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004709_1_0_1"/>
    <dbReference type="InParanoid" id="E3JXA4"/>
    <dbReference type="OrthoDB" id="413077at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045252">
        <property type="term" value="C:oxoglutarate dehydrogenase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004591">
        <property type="term" value="F:oxoglutarate dehydrogenase (succinyl-transferring) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006103">
        <property type="term" value="P:2-oxoglutarate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02016">
        <property type="entry name" value="TPP_E1_OGDC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1150:FF:000002">
        <property type="entry name" value="2-oxoglutarate dehydrogenase E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11610:FF:000009">
        <property type="entry name" value="2-oxoglutarate dehydrogenase E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12470:FF:000003">
        <property type="entry name" value="2-oxoglutarate dehydrogenase E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000002">
        <property type="entry name" value="2-oxoglutarate dehydrogenase, E1 component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11610">
        <property type="entry name" value="Multifunctional 2-oxoglutarate metabolism enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1150">
        <property type="entry name" value="TPP helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032106">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011603">
        <property type="entry name" value="2oxoglutarate_DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001017">
        <property type="entry name" value="DH_E1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042179">
        <property type="entry name" value="KGD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031717">
        <property type="entry name" value="ODO-1/KGD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00239">
        <property type="entry name" value="2oxo_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006914">
        <property type="entry name" value="PRK09404.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008907">
        <property type="entry name" value="PRK12270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152:SF4">
        <property type="entry name" value="2-OXOADIPATE DEHYDROGENASE COMPLEX COMPONENT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23152">
        <property type="entry name" value="2-OXOGLUTARATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16078">
        <property type="entry name" value="2-oxogl_dehyd_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00676">
        <property type="entry name" value="E1_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16870">
        <property type="entry name" value="OxoGdeHyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000157">
        <property type="entry name" value="Oxoglu_dh_E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="17">
        <location>
            <begin position="763"/>
            <end position="977"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00037426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00042984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00051911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EFP76679.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1133" mass="126240" checksum="9AA4EEE13AD09B4E" modified="2012-04-18" version="2">MPIVGSDRLSHPYTQVSPGLFSFGPKGNAGSEAEECLGCLGSAWIPGFDRGRVCCAGEIAYSPALPSTPTAPHSYSTTSHKNQQTKQINISPLSVSRTTHCPLATVLGNCHSVIMHRLNLNKIASAPRFLSNSLAPSNRLTSKFTKSSQNLRHLATATNDQVLRKAPSPVDGFVNSNNSYYIEEMYRMWRKDPGSVHASWNVYFSGLEKGLPSENAFRPPPGLVSMPQPAGGAPMLAMPGSGGEVEDHMKIQLLVRAYQVRGHHIAKLDPLNLSEADLQTIRPPEMDLKHYGFDENTDYEKEFSLGPGILPLFHTKDREKMKLREIIEACNRIYCGHIGLQYVHLPDRKECDWIRERVELPVPWSYSLEEKRMILDRLIWSDSFERFVASKHPNEKRFGLEGGESLIPGMKALIDRSVDAGVKSIVIGMPHRGRLNVLSNVVRKPMEAIFNEFAGSADASEDGGGDVKYHLGANYVRPTPSGKKVALSLVANPSHLEAEDPVVLGKTKALQHFDGEGSTDHAMGILLHGDAAFAGQGVVYETMGFHDLPHFGTGGTVHLVINNQIGFTTDPRQGRSTPYCTDIAKSIDAPIFHVNGDDAEAVTFVCQLAADWRAAFKKDVVVDIVCYRRHGHNETDQPSFTQPKMYQAIGQQPPTLKIYTDHLIKEGSFTEQENQQSQRMGVEYDGEGLRRKSGLQPNFARVAVELELKENILEARPTGVERSVMNKIGDTISGGWPENFEVHKNLGRILKNRGKTIAEGDQIDWSTAEALAFGSLLLEGNHVRVSGQDVERGTFSQRHAVLHDQKTNDNYIPLSNLKPEGSDPVGPFTICNSSLSEFGALGFELGYSLVDPHLLTMWEAQFGDFANNAQCIIDQFICSGERKWLQRTGLVMSLPHGYDGQGPEHSSARIERFLQLCDDDPFKFPTPEKAQRIHQDCNMQLVYCTTPSNYFHVLRRQIHRDFRKPLIVFFSKSLLRHPLAKSSVTEMEPGTFFIPLIPEPGFSGMVENHQIKRHIFCSGQVYYTLLQEREKRNINDIAITRIEQLSPVPYYEIVKALETYPNSDVMYCQEEPVNGGAYTYLAPRLENAMNQTENHAGKKVLYAGRPPYASVATGSKKIHKQEVQQFLDQALNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXL4</accession>
    <name>E3JXL4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">SWI5-dependent HO expression protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">SHE3</name>
        <name evidence="11" type="ORF">PGTG_02250</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">RNA-binding protein that binds specific mRNAs including the ASH1 mRNA, coding for a repressor of the HO endonuclease. Part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud and in the daughter cell. Required for the delivery of cortical endoplasmic reticulum into the emerging bud.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the SHE3 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76789.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EHS64104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321208.2">
        <property type="nucleotide sequence ID" value="XM_003321160.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890513.1">
        <property type="nucleotide sequence ID" value="XM_003890464.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXL4"/>
    <dbReference type="STRING" id="418459.E3JXL4"/>
    <dbReference type="GeneID" id="10528891"/>
    <dbReference type="KEGG" id="pgr:PGTG_02250"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02250"/>
    <dbReference type="HOGENOM" id="CLU_490132_0_0_1"/>
    <dbReference type="InParanoid" id="E3JXL4"/>
    <dbReference type="OrthoDB" id="6088208at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048309">
        <property type="term" value="P:endoplasmic reticulum inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031398">
        <property type="entry name" value="She3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051149">
        <property type="entry name" value="Spindly/BICDR_Dynein_Adapter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32123">
        <property type="entry name" value="BICD FAMILY-LIKE CARGO ADAPTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32123:SF9">
        <property type="entry name" value="PROTEIN SPINDLY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17078">
        <property type="entry name" value="SHE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="153"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="282"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="345"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="666"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="447"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="30"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="53"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="94"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="163"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="214"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="249"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="295"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="357"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="415"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="672"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="682"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00019884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP76789.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="691" mass="75562" checksum="CA29C5CF14672443" modified="2012-04-18" version="2">MDSKPASSSSSSRAHPSSRKTSISADSRRSLSISPTNSRTTHQKSQLPLPLRTQESNILSPSSTKFSQLKAFHRSVSSSSADQLAQRPPRTLSRKPSSTKTLPTKNSNCNFKSAQSTPPTPNGQPTHQNHQVTMLKEQLAKLQSELRIARGQVATLDNQLTRSKTPPSKTPPFGSSLSSSQRLQQQQQHPSSHSTQPSRAATPSRSPKSRKKSNSASKSHSLRKSSSSANSEQTDGLSIRFISGPGIHSSRSPQPSSIMDDSSLPISDSGYIKDPDGVWVPLNSPPAHSPSLNLSSSARYKSPSPSEAPRGISRIPISAVALGRVLAGDPLVPDHQSFQADIAEEQEPAEDHCDSVPRNSEYSSGASLSSPQIDDQNTTASTEPPGSHPPAVSRQPRGNPFFSSGGGRDRGAMIHQNSNADRTTNGGNTGKVITTLQSDLLYARTALDQSKSQLRLSQRAVESLNRQTEDLKESMSRLRLENEGLSKMLSRKERTVSELMDRLKRSEAELSTLKQEKKELDVNFKKISKETDEVVKDSVRRRDRAETQYEAVRSGVKSLSEGWKRDVTTLKQDICRLEEKHRKEMEDSRLKYNTLAKLHASRSGALSRIETDLNNLQSSKEGFIAKYTSELSGLRAHLEEEEKKSSEGLQLAKEVSDECARFKRILRNHSTGSSSSSSAAAPGPPHPKLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZ21</accession>
    <name>E3JZ21_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03252</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77296.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321715.2">
        <property type="nucleotide sequence ID" value="XM_003321667.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZ21"/>
    <dbReference type="STRING" id="418459.E3JZ21"/>
    <dbReference type="GeneID" id="10537841"/>
    <dbReference type="KEGG" id="pgr:PGTG_03252"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03252"/>
    <dbReference type="eggNOG" id="ENOG502RZSM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026393_1_0_1"/>
    <dbReference type="InParanoid" id="E3JZ21"/>
    <dbReference type="OrthoDB" id="3345970at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd11296">
        <property type="entry name" value="O-FucT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11350">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77296.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="621" mass="71821" checksum="7C4A96FE152698BD" modified="2012-04-18" version="2">MSYSIRNSRLTTPPATFNRLRNLTYSRTLRPNSNILIICLFILFIFTLSWILSNPKSINSPSWNVFNQINQFHPTQQTDTSYNTTIDPSHRFLVRDWSNYLGWNNFRYTVETGLLLAKLLNRTLVLPAFAYSRACEYDDNECTALTPILVNGVSVDVTSLSNRTWYPDPKPGTNIISPDLYLQYGKGWVLPIEKMLDIDHIISTWGHAIKLGDFHKLTNPDPSFHSIGVYDGTWEYQTSNRNLSYRKMPKAMFTNFSISMVDRLPKPIKPLIDRKKPSRPSSSALIAQCESTLKILEANVPQKRNKIYPPGPRRSDQDQRLPNWDESEIRGNYMVGKLAESDNPLLESCFASNGFRSAYAYGMLGWWMKAPYEPIKNIKRVKNMVGWWDELHKYDEQILHIEGELHNGFPPGQMIWTSLEGRQEFERLVRSVIRPPQWYHHVAARLEKKMRARCGGRSWVGAHMRRGDFLMFEWAANNITNQWKEIKMNTLAGANLLQSRPDLLRPTHLQFGTSLEPPHVDDPIYIATNIRQKEEVEYLRSKNIVLLTDLLEESDRVELGFSGKYMDTTSILEQCLLMRAGYYYGDAHSSVDGWILNRRVFYGISEEVTKIEYLKLPGDGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZ22</accession>
    <name>E3JZ22_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03253</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321716.1">
        <property type="nucleotide sequence ID" value="XM_003321668.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZ22"/>
    <dbReference type="GeneID" id="10537843"/>
    <dbReference type="KEGG" id="pgr:PGTG_03253"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03253"/>
    <dbReference type="HOGENOM" id="CLU_2293075_0_0_1"/>
    <dbReference type="InParanoid" id="E3JZ22"/>
    <dbReference type="OrthoDB" id="10285034at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="101" mass="10731" checksum="802FAD80C1777F1C" modified="2011-01-11" version="1">MDTAAGVDHARYALRPGTATRRPLDRPIEAEGRACGLWASVVLTTQRQENQQFADSPAGASGSAGGEVPWKRARPGENSPPALPTLTRPAWVLARKSQQAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZZ4</accession>
    <name>E3JZZ4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03575</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77619.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322038.2">
        <property type="nucleotide sequence ID" value="XM_003321990.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZZ4"/>
    <dbReference type="STRING" id="418459.E3JZZ4"/>
    <dbReference type="GeneID" id="10538827"/>
    <dbReference type="KEGG" id="pgr:PGTG_03575"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03575"/>
    <dbReference type="eggNOG" id="ENOG502SCBB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_715974_0_0_1"/>
    <dbReference type="InParanoid" id="E3JZZ4"/>
    <dbReference type="OrthoDB" id="9895617at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="341"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="255"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="282"/>
            <end position="297"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77619.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="387" mass="41511" checksum="416002C9617A9011" modified="2012-04-18" version="2">MNTNQEGGTATTTTGPASTSTQPSTTTAEAPTDSSWVSVVPLPPPPSYAEASLGASVAAPFSIPPSLHHPHPNQHTIETSHRFPSGLFVLRNRSSLKVLDVSGASTLVGTPVIAYNPKRPTLVNGDLLHKENNQLFFLDWHGCLCSASAGLRVDVSEEYGLKLTKPQPILSRPTRASHPPAEFRYDPMTRTIGVEFKHDPTYSNATMGEIHSVDYLLEVQPMSSGSTRKPSSSIINSPFPLEKLSEWFPFSAAKSTPSSSVQACSSSSPITGRRSMNGSSLDEGEEEEEDPDLDDSPDPARVVRVVSVAPGWREKFPSSGTPEARKWAKRQWDIASIIVQPAGPPSAESTTLRSDHPDPPISVLADLGEALGDLGHEIFRAFGGSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3K0D4</accession>
    <name>E3K0D4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03715</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77759.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322178.2">
        <property type="nucleotide sequence ID" value="XM_003322130.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K0D4"/>
    <dbReference type="GeneID" id="10540581"/>
    <dbReference type="KEGG" id="pgr:PGTG_03715"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03715"/>
    <dbReference type="HOGENOM" id="CLU_1326967_0_0_1"/>
    <dbReference type="InParanoid" id="E3K0D4"/>
    <dbReference type="OrthoDB" id="10564449at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77759.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="207" mass="23153" checksum="37375668D0F2084C" modified="2012-04-18" version="2">MNIPRKPLQGGSRQPASGPSRRGAGGEAPRGTQSARAPSVELFVPGLVLPSQEANRWNNELSGGIKWTHQSAQSSLSNIKYILEHLPPLISAHDAIPKEWSQRIWMDIDRTSKACETEDILKTVGNLSLIMSDMVDMEQIKTFQLTERLTGNHTLLPHLASFFAPGRSEYIRLRAALQQTWAMQKMTQMKDTWIPQAFDNSNNVYTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3K172</accession>
    <name>E3K172_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_04003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP78047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322466.1">
        <property type="nucleotide sequence ID" value="XM_003322418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K172"/>
    <dbReference type="GeneID" id="10541516"/>
    <dbReference type="KEGG" id="pgr:PGTG_04003"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04003"/>
    <dbReference type="HOGENOM" id="CLU_692859_0_0_1"/>
    <dbReference type="InParanoid" id="E3K172"/>
    <dbReference type="OrthoDB" id="10307917at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP78047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="352" mass="41252" checksum="3BF112191E39C425" modified="2011-01-11" version="1">MMNNFEIPVHIYRVYQEELLTWLDNQISPSHAESDPKLPVIGISELPGRVWPDSLHYGEAQLKLVNYFYNASREDHFLHSTANDLLDIFINTTHPIWLNQKTSVNFNPETDVVDALNRVLPSQTFQNNRNFISRLIKETSLFDKIFRLVRLTHRQENLSIKLLNEFKRQYKRTSFRNMNQIVHPKLPIATYFLVQYPEHKVMHILRHDKSNRSMQHEDVFQKLKKLMIAINCIHMKSFAYFGIKEAGFDASCETLDRWLISLILKAPGGIPILGSIKTEGELAPWEESAHPNLFSFVQLHLIKYFHEKQSPHNLKETALHVLNSWYEEHYPIRFQTLIQSTLSSKLLSTHAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2T5</accession>
    <name>E3K2T5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Lipoyl synthase, mitochondrial</fullName>
            <ecNumber evidence="9">2.8.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Lipoate synthase</fullName>
            <shortName evidence="9">LS</shortName>
            <shortName evidence="9">Lip-syn</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Lipoic acid synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_04610</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>[[Fe-S] cluster scaffold protein carrying a second [4Fe-4S](2+) cluster] + N(6)-octanoyl-L-lysyl-[protein] + 2 oxidized [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine + 4 H(+) = [[Fe-S] cluster scaffold protein] + N(6)-[(R)-dihydrolipoyl]-L-lysyl-[protein] + 4 Fe(3+) + 2 hydrogen sulfide + 2 5'-deoxyadenosine + 2 L-methionine + 2 reduced [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:16585"/>
            <dbReference type="Rhea" id="RHEA-COMP:9928"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:10475"/>
            <dbReference type="Rhea" id="RHEA-COMP:14568"/>
            <dbReference type="Rhea" id="RHEA-COMP:14569"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:33722"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:78809"/>
            <dbReference type="ChEBI" id="CHEBI:83100"/>
            <dbReference type="EC" id="2.8.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the radical SAM superfamily. Lipoyl synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.8" evidence="9"/>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323073.1">
        <property type="nucleotide sequence ID" value="XM_003323025.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2T5"/>
    <dbReference type="FunCoup" id="E3K2T5">
        <property type="interactions" value="350"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K2T5"/>
    <dbReference type="GeneID" id="10536507"/>
    <dbReference type="KEGG" id="pgr:PGTG_04610"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04610"/>
    <dbReference type="eggNOG" id="KOG2672">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033144_1_0_1"/>
    <dbReference type="InParanoid" id="E3K2T5"/>
    <dbReference type="OMA" id="RSCAFCQ"/>
    <dbReference type="OrthoDB" id="3231at2759"/>
    <dbReference type="UniPathway" id="UPA00538">
        <property type="reaction ID" value="UER00593"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016992">
        <property type="term" value="F:lipoate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009107">
        <property type="term" value="P:lipoate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
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        <location>
            <begin position="120"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="105"/>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
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        <location>
            <position position="110"/>
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        <ligand>
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        <location>
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        <ligand>
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            <position position="135"/>
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        <ligand>
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            <note>4Fe-4S-S-AdoMet</note>
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    <feature type="binding site" evidence="9">
        <location>
            <position position="139"/>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
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            <position position="142"/>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
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            <position position="353"/>
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        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047326"/>
        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP78654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
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</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3K5G0</accession>
    <name>E3K5G0_PUCGT</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    <gene>
        <name evidence="2" type="ORF">PGTG_05767</name>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <location>
            <begin position="1"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="EMBL" id="EFP79446.2"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
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</entry>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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        <lineage>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EFP79902.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="GeneID" id="10533593"/>
    <dbReference type="KEGG" id="pgr:PGTG_05127"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05127"/>
    <dbReference type="HOGENOM" id="CLU_3015248_0_0_1"/>
    <dbReference type="InParanoid" id="E3K6K5"/>
    <dbReference type="OrthoDB" id="10319565at2759"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP79902.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="56" mass="6122" checksum="4162EE1F01179DE4" modified="2012-04-18" version="2">MGTKEGSKSKGPYTCTEIGYNVACCKTAFKPGKHDISTLTDLKNGCVDPRNHNFQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3K6X8</accession>
    <name>E3K6X8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_05177</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EFP79952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324371.1">
        <property type="nucleotide sequence ID" value="XM_003324323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K6X8"/>
    <dbReference type="GeneID" id="10533806"/>
    <dbReference type="KEGG" id="pgr:PGTG_05177"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05177"/>
    <dbReference type="HOGENOM" id="CLU_1540840_0_0_1"/>
    <dbReference type="InParanoid" id="E3K6X8"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP79952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="174" mass="18649" checksum="BDB0126FD0B77C4C" modified="2011-01-11" version="1">MDGIFGGKANVTPLAQFDSGIGANLYGEMNKNSGRCDSSTEVFLSGWEETQEEQPPLGLTTPASQLDLSRRSSTLDWLTCQVTAALDGDHGVDEDDKLLPPIDFTGTAMDPSPSLMNQCLFAHATNGSGILNLASPMQSANMPQPLLARGKPWAARPFRINAVRTHPPPGLLER</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>E3K7C5</accession>
    <name>E3K7C5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Eukaryotic translation initiation factor 2 subunit gamma</fullName>
            <ecNumber evidence="2">3.6.5.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_06411</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="2"/>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80455.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324874.2">
        <property type="nucleotide sequence ID" value="XM_003324826.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K7C5"/>
    <dbReference type="FunCoup" id="E3K7C5">
        <property type="interactions" value="436"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K7C5"/>
    <dbReference type="GeneID" id="10539465"/>
    <dbReference type="KEGG" id="pgr:PGTG_06411"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06411"/>
    <dbReference type="eggNOG" id="KOG0466">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027154_0_1_1"/>
    <dbReference type="InParanoid" id="E3K7C5"/>
    <dbReference type="OrthoDB" id="1045173at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005850">
        <property type="term" value="C:eukaryotic translation initiation factor 2 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043614">
        <property type="term" value="C:multi-eIF complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990856">
        <property type="term" value="F:methionyl-initiator methionine tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045903">
        <property type="term" value="P:positive regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01888">
        <property type="entry name" value="eIF2_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03688">
        <property type="entry name" value="eIF2_gamma_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15490">
        <property type="entry name" value="eIF2_gamma_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000009">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000011">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000065">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050543">
        <property type="entry name" value="eIF2G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015256">
        <property type="entry name" value="eIF2g_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044127">
        <property type="entry name" value="eIF2g_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044128">
        <property type="entry name" value="eIF2g_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003077">
        <property type="entry name" value="PRK04000.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09173">
        <property type="entry name" value="eIF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 12" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
        <location>
            <begin position="84"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP80455.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="513" mass="55739" checksum="5D78FADD564BC932" modified="2012-04-18" version="2">MATSNDPVDLSDDGSDSSHSDSAQHQQPNSNSNSNNQNHKPNGTNDLNSKPIRPNEHINIEDIKEIKIDTSSLNPLSPQVISKQATINIGTIGHVAHGKSTVVKAISGVHTVRFKNELERNITIKLGYANAKIYKCEEASCARPGCYRSYGSDKEDRPTCDVPGCHGRMKLIRHVSFVDCPGHDILMATMLSGAAVMDAALLLIAGNEPCPQPQTSEHLAAIEIMKLKHIIVLQNKVDLITEAAASEQYKSILSFVKGTVADSAPVVPISAQLRYNIDSVNEYIVKKIPVPMRDFSADPRLTVIRSFDVNKPGAEVDELKGGVAGGSLLQGVLRMGQEVEVRPGVVTKDSEGKIHCKPIRSKIVTLLAEKNELLFAVPGGLIGVGTKIDPQLCRADRLVGQVLGAVGKLPKIFTEIEINYFLLRRLLGVKTDDKKQTKVSKLTKGEILMVNIGSTSTGGRVMSVKADLAKVLLNQPACTEVGEKVALSRRIEQHWRLIGWGSVRRGAEYEIHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>E3K7G6</accession>
    <name>E3K7G6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_06426</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80470.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324889.2">
        <property type="nucleotide sequence ID" value="XM_003324841.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K7G6"/>
    <dbReference type="STRING" id="418459.E3K7G6"/>
    <dbReference type="GeneID" id="10539480"/>
    <dbReference type="KEGG" id="pgr:PGTG_06426"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06426"/>
    <dbReference type="HOGENOM" id="CLU_309967_0_0_1"/>
    <dbReference type="InParanoid" id="E3K7G6"/>
    <dbReference type="OrthoDB" id="308383at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050973">
        <property type="entry name" value="H3K9_Histone-Lys_N-MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003616">
        <property type="entry name" value="Post-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007728">
        <property type="entry name" value="Pre-SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46223:SF3">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SET-23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46223">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE SUV39H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05033">
        <property type="entry name" value="Pre-SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50868">
        <property type="entry name" value="POST_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50867">
        <property type="entry name" value="PRE_SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Pre-SET" evidence="10">
        <location>
            <begin position="809"/>
            <end position="912"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="9">
        <location>
            <begin position="915"/>
            <end position="1104"/>
        </location>
    </feature>
    <feature type="domain" description="Post-SET" evidence="11">
        <location>
            <begin position="1126"/>
            <end position="1142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="190"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="368"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="432"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="581"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="678"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1010"/>
            <end position="1039"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="190"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="206"/>
            <end position="232"/>
        </location>
    </feature>
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        <location>
            <begin position="271"/>
            <end position="280"/>
        </location>
    </feature>
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        <location>
            <begin position="282"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="292"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="368"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="392"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="444"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
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            <end position="478"/>
        </location>
    </feature>
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        <location>
            <begin position="488"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="504"/>
            <end position="533"/>
        </location>
    </feature>
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            <end position="549"/>
        </location>
    </feature>
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        <location>
            <begin position="554"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="679"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1010"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1026"/>
            <end position="1035"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50868"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP80470.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1143" mass="125344" checksum="05628649D585F296" modified="2012-04-18" version="2">MCINLGRIRCDFLVFSWLLPQLRVPRTPYGHTQLNKATRTEVRGNRSHILSRNTELSLSYHHPPTANSLTFVSSDEVARPQVQPITHSDSALYTPATGSSRLEFGSLLRRHSNGASGEPARRSHILSRNTELSLASSSADYAFRFRIVHACHWVPSRLEFGRIAAFGSAMHGATNGNPVTNSTVNRSLIASRPSSSTVDRSLIASRPSSSTVNQSLIASRPSSSTKNPTSRSPPEVITINTDDEDEHIKITSAPKAKPRTSTKPTCGLDVSSWNVGTKSKPNGHDKLKDSSSRPILSSSTNCLNHVDQTQEPKSIQPHTSSSSTNHRDSCSSSQPDFAGISKSKQPNLHARELTKERSQHANLMNTAHSKGTLNSSKSNSHPTHVSKITEDAPQSTKQGESQHLSQARTTKHSNGQNDVINKLNFFSYLPSSGSGNSSMRKEQPSGSSASTSNLSKSGTNKSSKKQDSDTSWATSALKQSMVRPVHARQQEENAKRMELKRLQSRGSASAVGTESNRCTQPGSSVLTGSTTTHSIIRSSGSNVTSSTSSALAKGNNNTSSMCASNQGDNTFSQKILKAASTVPPNGINASSEAPHAPKKRKHMSPPQSKKLEERFVELSNADPMQPVPSASCPPPRKSISNVVPLEPGPQLKQNSSFGSNINNPTHTNVNPCVIHPVATLPPPKVTPRPRVPSNRRPLQGRERPDEFSTELQRAWILTNNPDLTTPLGHLIFSEEINANQMWENQEIPHIDVVVPDEVKAQMLQDSKAAALDEEGKLTGLDQSLAPPLEFVYTDRVVYRNNERPIPPTWQCQCEGDCSDDPNCECRIYQTKMVRQTAEALGVNLNESVTNFEGFAYVSNRKPRHSHFKKPDNGDLEARSVAGLFLDHSFPVFECHSKCGCGPNCINRTVGRGRREKLSIQKTLSRGWGVFADHPIPTGRLVTHYSGELITDAMSDERSRSKYDKIGRTYLFDLDPWWIETIDSNSLANEGLIVISETKQFSEAHKRLTSGGSLQNVTGPLFNSSSKAKKKQSKSQKPKETDIDGVYSVDAFLYGNVSRFINHSCSANTVIVPVYIEDSDPTRPIFAMFAKKNIKTGTEITTSYSDPNDEEYKRLETGQMNNENHALFMPCKCNAPNCKGIMFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3K7L6</accession>
    <name>E3K7L6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_06449</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80493.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324912.2">
        <property type="nucleotide sequence ID" value="XM_003324864.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K7L6"/>
    <dbReference type="GeneID" id="10539504"/>
    <dbReference type="KEGG" id="pgr:PGTG_06449"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06449"/>
    <dbReference type="HOGENOM" id="CLU_1971602_0_0_1"/>
    <dbReference type="InParanoid" id="E3K7L6"/>
    <dbReference type="OrthoDB" id="2505635at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052035">
        <property type="entry name" value="ZnF_BED_domain_contain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481:SF10">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46481">
        <property type="entry name" value="ZINC FINGER BED DOMAIN-CONTAINING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP80493.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="127" mass="14725" checksum="B35DB960E238B74F" modified="2012-04-18" version="2">MNIGSLITPPNGTGINTHTILMRIHGLCTWVCFSPQRREKFTIDVKYCQPEMCAKKIQGLEIDVPTHWNSNYSMLQRALSLEKTCTHFFKHNAEASKFLLSPAEWDQARYLTQLLEPTVQQVLYTYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3K7M5</accession>
    <name>E3K7M5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hydrophobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06279</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80323.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324742.2">
        <property type="nucleotide sequence ID" value="XM_003324694.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K7M5"/>
    <dbReference type="GeneID" id="10539331"/>
    <dbReference type="KEGG" id="pgr:PGTG_06279"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06279"/>
    <dbReference type="HOGENOM" id="CLU_2122270_0_0_1"/>
    <dbReference type="InParanoid" id="E3K7M5"/>
    <dbReference type="OrthoDB" id="2508336at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172428" description="Hydrophobin" evidence="1">
        <location>
            <begin position="23"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP80323.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="12965" checksum="1236D5D8EAF6E9B4" modified="2012-04-18" version="2">MQSFNLLIVFAAVLLINTQVNCGSFVCPIPGQPEDQKDAYCIRRITREELRDPANKNATMFKLHITEKGEDGFTCDDVILNGKPARDSFCCNAPGDIGVVLPSPQSMWDNKCSIIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3K7M6</accession>
    <name>E3K7M6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_06280</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80324.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324743.2">
        <property type="nucleotide sequence ID" value="XM_003324695.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K7M6"/>
    <dbReference type="GeneID" id="10539332"/>
    <dbReference type="KEGG" id="pgr:PGTG_06280"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06280"/>
    <dbReference type="HOGENOM" id="CLU_2074294_0_0_1"/>
    <dbReference type="InParanoid" id="E3K7M6"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP80324.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="118" mass="12819" checksum="3F53219D34F44F34" modified="2012-04-18" version="2">MWPSTCGGVSLESKYATWSLRRPVGEPSKLSGYRAIDHRPGPVDEESDGPSMLNLLQPYCPCGLLSGGGAWGVKAVQHPPSRPMSLGHKAMTICLQCCWPFPRGQDNLALGRSFSLNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3K9L4</accession>
    <name>E3K9L4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RNA polymerase II-associated protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_07362</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <text evidence="3">Belongs to the RPAP3 family.</text>
    </comment>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07362"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR46423:SF1">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <location>
            <begin position="11"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="80"/>
            <end position="113"/>
        </location>
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        <location>
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        </location>
    </feature>
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            <begin position="136"/>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF13877"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP81110.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
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    </evidence>
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            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81266.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003325637.2"/>
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    <dbReference type="GeneID" id="10536753"/>
    <dbReference type="KEGG" id="pgr:PGTG_06887"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06887"/>
    <dbReference type="HOGENOM" id="CLU_2321504_0_0_1"/>
    <dbReference type="InParanoid" id="E3KAA9"/>
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    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP81266.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="99" mass="10799" checksum="967D4B2F4A23E5EF" modified="2012-04-18" version="2">MPGAVPAYFNPSLSASTIQGRPHPPIGSRPTHLKRANELVRLSKKRVVDTQRGVGIANLSLADTFAQLAKANKIKDHLVFQALEGCRPVWDGWLNNNSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAB0</accession>
    <name>E3KAB0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_06888</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81267.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325686.2">
        <property type="nucleotide sequence ID" value="XM_003325638.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KAB0"/>
    <dbReference type="STRING" id="418459.E3KAB0"/>
    <dbReference type="GeneID" id="10536754"/>
    <dbReference type="KEGG" id="pgr:PGTG_06888"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06888"/>
    <dbReference type="HOGENOM" id="CLU_1343848_0_0_1"/>
    <dbReference type="InParanoid" id="E3KAB0"/>
    <dbReference type="OrthoDB" id="727118at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051488">
        <property type="entry name" value="WD_repeat_striatin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15653:SF0">
        <property type="entry name" value="CONNECTOR OF KINASE TO AP-1, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15653">
        <property type="entry name" value="STRIATIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="7"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="177"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="188"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP81267.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="204" mass="22029" checksum="C7BA023F9F1E7D32" modified="2012-04-18" version="2">MEPQITYRGHSATITSVAISSSPPRIYSASLDSVVLVWSLPPPEHETYAPYDPRSLLASFVGHTDVIWDMVLLPLRLRDEALLETVSADGTVKVWSTDKLNSPLKLSWGYDGFIDEDLPQASQPTPSNGLTVIDANGSPVKVTPTSVAVVWSDLKKVAVSYSDSVIQLVGRGSSRRAGLKSSLSEGTPSEKLDHARRDGFLPAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAW1</accession>
    <name>E3KAW1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_06972</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81351.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325770.2">
        <property type="nucleotide sequence ID" value="XM_003325722.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KAW1"/>
    <dbReference type="GeneID" id="10536923"/>
    <dbReference type="KEGG" id="pgr:PGTG_06972"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06972"/>
    <dbReference type="eggNOG" id="ENOG502SUWC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040573_0_0_1"/>
    <dbReference type="InParanoid" id="E3KAW1"/>
    <dbReference type="OrthoDB" id="2498727at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040223">
        <property type="entry name" value="PAR_bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988:SF27">
        <property type="entry name" value="GH27708P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988">
        <property type="entry name" value="THYROTROPH EMBRYONIC FACTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP81351.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="470" mass="53512" checksum="E6E9106AD4BC564E" modified="2012-04-18" version="2">MAILSDLSEELVHRVIDHIVLPQNDNNQLKGCHLHLDHIQDRPRAHLEHKNNDFRTSHRDNNNETLAQVSWPEGLPSNKLLPLSLINRTFRQCAQERLFQNVALHNQWQGCLFLHELQEGDIEIGSSSDTKHPRLSPLAQHVHSLLLKDWSDHKPPSMGRGGGSLICETIRSCPSLENFAMVNSLDGRCKDPIMDALASRKHIKDFVVLKDFYGHPCNQKIIWLADEVVARLFAKWEHLEKIELHKLSGRPIEMIETIHKALESLRTLEIVNPTPKLDRPGLCRILKECTGPDLESLTVATLPNWHIIRSSVNNEISDDPAENRNLLEILFKSSSALKNLKSLSIQGRLTGSELFIFLPPSIVKLSWGRCHLTAAAFAKALSSFIQYPPELPSDLKLDHTDVLNRRVQWLPKLKCCTVIDDYDWDKQERDAIDKALRARRVCFHSGGNYYAESEGDENHLKSKVLDTVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAZ7</accession>
    <name>E3KAZ7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_06997</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81376.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325795.2">
        <property type="nucleotide sequence ID" value="XM_003325747.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KAZ7"/>
    <dbReference type="GeneID" id="10537029"/>
    <dbReference type="KEGG" id="pgr:PGTG_06997"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06997"/>
    <dbReference type="HOGENOM" id="CLU_083965_0_0_1"/>
    <dbReference type="InParanoid" id="E3KAZ7"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16448">
        <property type="entry name" value="RING-H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051826">
        <property type="entry name" value="E3_ubiquitin-ligase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765:SF416">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE GODZILLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765">
        <property type="entry name" value="RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012655222" description="RING-type domain-containing protein" evidence="3">
        <location>
            <begin position="16"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="232"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP81376.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="289" mass="32890" checksum="77D8F9A9186F3A7A" modified="2012-04-18" version="2">MKLFVLSIFCSVLLAMDLQNLSGDLRYHLGDGAHFSEEIRATSEVEQRITPSNRDRTANRLRFPGSSLFDRIRHRNSGRDQGRATLQAAPEPPEITPEQEYMFQRYQQQGVNLLYIAPEQALSEVEHLRLVQAGPPTGPRSYKETDKIENTRLLGYMLKPSPSIRQDTVPLEQALKTLPLSSNAALQRSHRIGLRTSGNGPHDYHDGSSSTSDQSLDEITEVLLADPDHRKCVVCLEKFKSPQRDGKNRWSFDKVLMIDGCKHYFHRSCLQTWVLGRGHRDCPTCRHEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBM7</accession>
    <name>E3KBM7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_07761</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAPT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81512.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325931.2">
        <property type="nucleotide sequence ID" value="XM_003325883.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KBM7"/>
    <dbReference type="FunCoup" id="E3KBM7">
        <property type="interactions" value="58"/>
    </dbReference>
    <dbReference type="GeneID" id="10537250"/>
    <dbReference type="KEGG" id="pgr:PGTG_07761"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07761"/>
    <dbReference type="eggNOG" id="KOG2490">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003655_1_1_1"/>
    <dbReference type="InParanoid" id="E3KBM7"/>
    <dbReference type="OrthoDB" id="2500787at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008010">
        <property type="entry name" value="Tatp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13317">
        <property type="entry name" value="TRANSMEMBRANE ANTERIOR POSTERIOR TRANSFORMATION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13317:SF4">
        <property type="entry name" value="TRANSMEMBRANE ANTERIOR POSTERIOR TRANSFORMATION PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05346">
        <property type="entry name" value="DUF747"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="223"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="293"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="374"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="676"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="138"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="756"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="35"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="72"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="138"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="157"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="757"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="772"/>
            <end position="782"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP81512.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="830" mass="94623" checksum="C42EDDD1D4F4945F" modified="2012-04-18" version="2">MNSDPTQSESGTNISNTDETGREGLYHHHTQQEGTTEEEDQAEPIDTDDDDDEEEEAESLVLIQSPKQEIDHQEEEADDDDEQSTCDSKTHSSPTHRNHIKQEHHSGQSTPDYHHHQRNITSPESEVLEDSLIYETQHQVDSPNQSPSPNHQDRSNQDSLNRNQSTQASINPSFSLWEHLKYEISSNQDDLSPLQDSPSASLRSERISNFLNVPIAIEKTIVFGFWICLDSFLSVLTILPIKFLYSLTKFCQSLAYKTAHSTRIIKNKFLGRGSNDERSKHPHRKRLSVPCKVDLLQGLLIILVCVFLHHVTDASRMYHSVRGQETIKLYVIFNVLEIADRLCCSFGQDILDSLFSPSTLGRRLDGSQPHMKPIFLFILAFIFTVAHTLVLFYQLVSLNVAINSFDHSLITLLISNQFVEIKGAVFKKFEKENLFQMSCADIVERFQLFLMLAIIAIRNLIEMSGSSTSSSHAHSYTYHYLPSSLNLSPTLSLIEKIFTPVIVVMLSEVLVDWLKHAFITKFNHIRPSVYGRYIDILCKDLVGDHKIVRVEDSMRDDEDIKPFVDKSPAVSRRLGFSVFPIFCLSVRVSFQAIDMLSDTPDEEFNMFTDDLMDVLVDQPSKFSVEYIKQQIFTIPYSKFFNADPAAYLDPSKQDRSSPSRPEVSGSSTFSNFQMKGFIILSFVVVMSFLILIKLYIGIRIRLYAIKRVQTMNRRLAEDQVNGRDRLPIGLSPTEIMKEENDRKLIEKVEFDVPLRRISSSSKPAPNSNQESVDEKPTGDQLRKIPSGLLLAPSCSHPPPNSLDQPSSQHKKKPLSMDELSRFDMVRSRIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBX7</accession>
    <name>E3KBX7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="7">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_08028</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="7"/>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EFP81779.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326198.2">
        <property type="nucleotide sequence ID" value="XM_003326150.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KBX7"/>
    <dbReference type="STRING" id="418459.E3KBX7"/>
    <dbReference type="GeneID" id="10533644"/>
    <dbReference type="KEGG" id="pgr:PGTG_08028"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08028"/>
    <dbReference type="eggNOG" id="KOG0352">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011478_1_0_1"/>
    <dbReference type="InParanoid" id="E3KBX7"/>
    <dbReference type="OrthoDB" id="10261556at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="52"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="697"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="846"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="653"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="697"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="857"/>
            <end position="877"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP81779.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="877" mass="95557" checksum="635CD9E57CB2BEB4" modified="2012-04-18" version="2">MALIEMETVSAKKNRLPDGLSDMAEAALLEHITSRSVELYADQPKGLQVEAVSSLVRRGHTFVRAGTGFGKTRISEMYFGLFDRKVVVLVLVPLDSLGDDQVREKKLVNITAINLNKMTLNRASIREIKKGKFSFVYLEFQAILALIVLDEAHMVYLWGLVASKHSKSLIIFARLEDQAIFRPAYGHIGTRLMATNDVPLLLLSATCRPEAVDAITSSLMLQPSELSMIDGELTRPEIRFVRIYMEATLSSCDDLLRVFAPHTTTPAAMAVPTIIYSGTRNRTFQVMKVVNEARDTKMHEYGPKDEFILRFHAVTGDQDKNQTISNFGAASVPVISATMALGLGQNLKRVRCVIHMGRGDPPAIVQMVGRCGRDGNVGLGLLFMEPTRKNGKNKIEDFEEGVRQDDDARMDALALTKCCLRIALALDNKIGYIPLSDSDPEVKAEAAREIQLGFAKCKCSNCLPAEAEALMKVFQQANQDNFDSMLDDPYSVVKDPGLVTMTRKRKTITTKATCGYPASVAQDLVQHLIHHHEAFYGAHLGPKAEFPASVFFGVDQAKAIVGSIDQIRSAEGHNTELMEALIGGQCFPGQINSLDQAISDWMNSEFYHLHLSQVASLDQFIEAEGIRVREKMAVELEHLQKQAIARRATEKAAKDAAKALAKAEIAARKATERLAAAHDKAQEKLLLAAEKAAEKARHADEKKAAKAQAARDKANGRAGFNGRAGAGAVNGGLISTSNGVNTDIPGGAGHMDSNENKRARVVEENKTNEIGKRKHWADRRAAADATRAADALAKAEGIRQQREGVMMKSMGAQVKRASRGWAHMAKVLRKSQVNADIEGIRASLAMGENQGSPMGQFSSAGSFSASPSVPESSSLGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KC41</accession>
    <name>E3KC41_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ecp2 effector protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08248</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EFP81999.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326418.2">
        <property type="nucleotide sequence ID" value="XM_003326370.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KC41"/>
    <dbReference type="GeneID" id="10534365"/>
    <dbReference type="KEGG" id="pgr:PGTG_08248"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08248"/>
    <dbReference type="HOGENOM" id="CLU_095050_0_0_1"/>
    <dbReference type="InParanoid" id="E3KC41"/>
    <dbReference type="OrthoDB" id="10274472at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173734" description="Ecp2 effector protein domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81999.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="249" mass="27477" checksum="A6BD89D718F8F076" modified="2012-04-18" version="2">MALHRVVLVVVISLLNLQSSLQQTKPPSLTDIKCGDKKEFKAGDCAAAYRKINYDKDSTLDIGESSVERSSESCITRINNPKFMNVPKTTIENGFDQILAKCNGYAGSATLPGFNGVRLFTSHHVGPDFRSYDDYKEFNQMICNGDYPKGAKVLKEDCMEAYRLIPTNAAGHFVSLNHHVPTSTIMSVAKKCNAAIWTSDGSKIMLLKTDIDKIFGKMMQECPIWGGHFLTKGASGKNGVVIFQVWGRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCI2</accession>
    <name>E3KCI2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_08179</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EFP81930.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326349.2">
        <property type="nucleotide sequence ID" value="XM_003326301.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCI2"/>
    <dbReference type="GeneID" id="10534130"/>
    <dbReference type="KEGG" id="pgr:PGTG_08179"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08179"/>
    <dbReference type="eggNOG" id="ENOG502QQR9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048932_1_2_1"/>
    <dbReference type="InParanoid" id="E3KCI2"/>
    <dbReference type="OrthoDB" id="78198at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48471">
        <property type="entry name" value="DDE TNP4 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48471:SF1">
        <property type="entry name" value="DDE TNP4 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="4">
        <location>
            <begin position="222"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP81930.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="437" mass="50498" checksum="4808B329687DF6DD" modified="2012-04-18" version="2">MDNPESNAEATEDGRRRDQRRRRTYQSNLQQHASKRRREEEDLIMVMYGMGLQGAPGKFRQLRTYLTQSDLPTSTNSAWTTMMKARNDRAYMTTMGVDVTTFEDILSRFELLWNSVTLPRNNVNPNGEPQVGRRSLDAAGCLGLVLHWLCSTMAAYTLQQIFGITPAVCSRYLDWGLDCLLQVLKNHPQARIIWPSTKERIQPYATTIKKKFPLLENCFGFLDGLNLPICVAEDDDLQNAYYNGWTCSHYCSCIIAFAPDGTIMYAILNAPGSWHDSAIAVPLYERLLNHTPKGYRIISDTAFPRKSQRLQRQILAPVKRGDRLPETPRSFSRMKLLNEEVVSARQAAEWGMRSLQGSFARLKLLMPAADHSQRLRILQAVCYLHQIRCRMVHINQTETVYDSVWDEHQVLSRRFHNLLFKDIEKRCHISRYYQGWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>E3KD73</accession>
    <name>E3KD73_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_07503</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326525.1">
        <property type="nucleotide sequence ID" value="XM_003326477.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KD73"/>
    <dbReference type="GeneID" id="10534637"/>
    <dbReference type="KEGG" id="pgr:PGTG_07503"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07503"/>
    <dbReference type="HOGENOM" id="CLU_1200328_0_0_1"/>
    <dbReference type="InParanoid" id="E3KD73"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP82106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="231" mass="24763" checksum="262C53E681736F74" modified="2011-01-11" version="1">MPKDSGFTFLHTRESSPQRQFVPRGIVEPTKLGRSVEPTRSALRSGSRCSLKITWNSIRTGRYDLEKALGTTSSLWTSRQGSPLTDQLTLSPGFKSQVFPFLRPPAVSATAALPKSRRLLGAASEECRGMGGWGPRKTLDRQVLVRLPASGHSWDPQAKGVSDHRRTAPQVYCQHHGSGGRVLDGSGSVGLTHAALTKILGCFIAGGVIPRGRLDPSTSACQRLPGKLAAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3KF23</accession>
    <name>E3KF23_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_09822</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="DS178284">
        <property type="protein sequence ID" value="EFP82854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327273.1">
        <property type="nucleotide sequence ID" value="XM_003327225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KF23"/>
    <dbReference type="STRING" id="418459.E3KF23"/>
    <dbReference type="GeneID" id="10544564"/>
    <dbReference type="KEGG" id="pgr:PGTG_09822"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09822"/>
    <dbReference type="HOGENOM" id="CLU_018552_9_1_1"/>
    <dbReference type="InParanoid" id="E3KF23"/>
    <dbReference type="OMA" id="NDSHTLI"/>
    <dbReference type="OrthoDB" id="2506086at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="3">
        <location>
            <begin position="8"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP82854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="157" mass="17730" checksum="ADA4B366EE5644D0" modified="2011-01-11" version="1">MKVHLELNRFFDSGQYLIADSAYGLSMTTIPAYKSPLSKITRNTKFNYCLAKSRVRNEHTIGILKGRWASLQQLCLSLYTPQHMLEIIRWINCCVALHNMLAHLGDSWDDLAVSNDNDGPVGPQVNDEDDDTGHCEEIQNKCLIFNHLNGTLPIPSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>E3KFJ9</accession>
    <name>E3KFJ9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">E3 ubiquitin protein ligase</fullName>
            <ecNumber evidence="15">2.3.2.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PGTG_09152</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H2B to form H2BK123ub1. H2BK123ub1 gives a specific tag for epigenetic transcriptional activation and is also a prerequisite for H3K4me and H3K79me formation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 15">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the BRE1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.27" evidence="15"/>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EFP83199.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327618.2">
        <property type="nucleotide sequence ID" value="XM_003327570.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KFJ9"/>
    <dbReference type="FunCoup" id="E3KFJ9">
        <property type="interactions" value="245"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KFJ9"/>
    <dbReference type="GeneID" id="10544912"/>
    <dbReference type="KEGG" id="pgr:PGTG_09152"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09152"/>
    <dbReference type="eggNOG" id="KOG0978">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019713_0_0_1"/>
    <dbReference type="InParanoid" id="E3KFJ9"/>
    <dbReference type="OrthoDB" id="10266039at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033503">
        <property type="term" value="C:HULC complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16499">
        <property type="entry name" value="RING-HC_Bre1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058643">
        <property type="entry name" value="BRE1-like_CC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013956">
        <property type="entry name" value="E3_ubiquit_lig_Bre1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23163:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE BRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23163">
        <property type="entry name" value="RING FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08647">
        <property type="entry name" value="BRE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26095">
        <property type="entry name" value="CC_Bre1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="11 15 16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 15" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="9 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="7 14 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="18">
        <location>
            <begin position="787"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="215"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="728"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="71"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="352"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="487"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="256"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="293"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="728"/>
            <end position="754"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00059679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU365038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EFP83199.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="839" mass="94473" checksum="7663A8B7D28C3B6A" modified="2012-04-18" version="2">MLDDQRKRRIELNAGPTARKKPMLNNSSTPSPILDPASNHLNSTDRTHQSESANHLNQSVGDDDLLENFRKEALWRQLQEYKRDLQRAQERIEQSESQKSDVESRLAAIDICWNQLIEDVRVLFKDSCTNLDSSTHATLMSLPSSPTSLDRALHQRSTASKDLIIHLISLASSSSVASPDLEEIRARCRRLTAESASNRAALGLAESKLKQLQDQLDAAQEATRRAEKKLDRERSRTVKELDAQGQGGSTSQAPKGETRLSSDDSPKPLVEQNGNGTHAVKPPSQPVCNGTDPLDHDSSKEASNDRSRELEILNEQLLQRAHEIDILNWKMINLPDEIVRNTPAFTRLKAEVAHATLELERVRALLETTQKEADELRERQEEFRTKIVAEMTKRVEDMEKKLASKDADVTRIRATREEARSELHELKSRDVEKMKQVAQIRTLANSRQDRINALSSEVRRLKMRLAADVGDRNTVDFLATNEEVDLAKALQERVKIAEETIKSLNNQIEELAQNSKSEDSASLIRSEAEARQELVAARQRIESIDRLLGPDGNSDSSEMAKKLQVELDRQQVLEAKLAAQEASASMLYTEVDRLSTAWAVLDEQNRDKVFKLAEHDEKVLKAVHEKAKADNRYFSAMRANETLKSQTAVLEKMAEKQTVTISKLSDELRAVSARLTSAEKEITIHQKMAEAHKTRTTELSIENADLIARCSQHDQKVIELSTLLRDRTTQAETEASARRSAEESVTNLEKEMQRSKLTGSNGRGGLEPAEVAELRSFNDDLTKMLKCNSCKQRFKSHVITRCMHLFCGACLEARLETRQRKCPTCSIAFGTNDVSAVYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3KFK2</accession>
    <name>E3KFK2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Lysophospholipase</fullName>
            <ecNumber evidence="2 9">3.1.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_09155</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the lysophospholipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="2 9"/>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EFP83202.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327621.2">
        <property type="nucleotide sequence ID" value="XM_003327573.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KFK2"/>
    <dbReference type="FunCoup" id="E3KFK2">
        <property type="interactions" value="216"/>
    </dbReference>
    <dbReference type="GeneID" id="10544915"/>
    <dbReference type="KEGG" id="pgr:PGTG_09155"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09155"/>
    <dbReference type="eggNOG" id="KOG1325">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014602_1_0_1"/>
    <dbReference type="InParanoid" id="E3KFK2"/>
    <dbReference type="OrthoDB" id="4084751at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000030">
        <property type="entry name" value="Lysophospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF33">
        <property type="entry name" value="LYSOPHOSPHOLIPASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8 9" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6 8 9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005127806" description="Lysophospholipase" evidence="9">
        <location>
            <begin position="25"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="domain" description="PLA2c" evidence="10">
        <location>
            <begin position="64"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP83202.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="620" mass="67982" checksum="0BCC9C28E98E1FA4" modified="2012-04-18" version="2">MRCRSWQVLVTILLDVCISHHSVSISFSFPRPQFNKILDRRRLFKRDQAIPTAPSGGYAPSRRPCPEKVLVRQPSVHGPLNSGEAEYVLTKAQKSLPLWRTYLENAGLLGFNVDDFLSEATQNGGTPAVTLPNFGFAISGGGARAGLVGAGILNAFDSNNPAAVEAKTGGILQLANYAAGLSGASWLLGSWATANFPSFTSLNQTVWKLTQPDAIYDIAIIKQIHRDLKTASQKAMAGFPISIVDAWAQLIVDHTINTTHHANAVLLSSVKDLPGFKSRYAPFIIITATSRENGKEEMSLDNPVYEFTPEEFGTWHPSLNAFIPVQFLGTKINQGQISRGDRCVVGFESMGFIMATSSNIFSTSEKTSDDPFWVALVHKFMNYMTGNVYDEAIIPNPFQGLGLGFGLDGGFPSKDDENLYLADSSLSGETIPLWPLIQPSRKLDAIITVDSSNRAKPSVKSRVYPNGTSLYASYRKILPPDYAAYPFPTVPDPYGGNFSRLGYNKRPVFFGCDQACPLIIYLPNYFIVAPTDAPTTQMQYSNTDIDGYFKNGFALATQTRASANSMSEDMQGLFDRAGPSSSIEWSICLACALIDKQQKRNGRRRTAQCQSCFDMYCAAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHD2</accession>
    <name>E3KHD2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ccr4 associated factor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_09420</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the GcvT family. CAF17/IBA57 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328126.1">
        <property type="nucleotide sequence ID" value="XM_003328078.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHD2"/>
    <dbReference type="FunCoup" id="E3KHD2">
        <property type="interactions" value="135"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KHD2"/>
    <dbReference type="GeneID" id="10532394"/>
    <dbReference type="KEGG" id="pgr:PGTG_09420"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09420"/>
    <dbReference type="eggNOG" id="KOG2929">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007884_7_0_1"/>
    <dbReference type="InParanoid" id="E3KHD2"/>
    <dbReference type="OMA" id="NMLVAND"/>
    <dbReference type="OrthoDB" id="191995at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.120:FF:000050">
        <property type="entry name" value="Putative transferase caf17 homolog, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.120">
        <property type="entry name" value="Probable tRNA modification gtpase trme, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027266">
        <property type="entry name" value="TrmE/GcvT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017703">
        <property type="entry name" value="YgfZ/GCV_T_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045179">
        <property type="entry name" value="YgfZ/GcvT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03317">
        <property type="entry name" value="ygfZ_signature"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22602">
        <property type="entry name" value="TRANSFERASE CAF17, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22602:SF0">
        <property type="entry name" value="TRANSFERASE CAF17, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103025">
        <property type="entry name" value="Folate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00093447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP83707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="403" mass="45144" checksum="E3A51267F642F9CB" modified="2011-01-11" version="1">MRPVILTTPRSVSVGLSGHSHSFHSSSRATEWLRTRLVDRGLISLKGEKSKTFLQGLITNNLNRLSSQEEAHHNTAFYTAFLTPPGRLQFDGFVYPEPPENEAQCLLIDHHLPEADRLLAWLRRFVLNSRVKISKDSRELWAVWPNHPLDNIQSLLPNSSLLEQPSNHAWKDHRGDQRLGYRIIGNPESVPELEPLAQLPEAPLSAYALHVLLHASLPPSLSPPYPVTLPFEANLDYHHGVDFRKGCYVGQELTARTYHTGVIRKRMVPVSIEKDGLPALASVPDRLSISDTQMADRPLTERLEGHELRLGSQPPNTSTIPAQKQKTIGKLCGPFISTSPVNHHSSDGPRSLLYGLALLRIDHDAFSPSVEHPWPTISLSTVDQDLSPWTIQPIKPVWWPSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHE6</accession>
    <name>E3KHE6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6589 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_09434</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83721.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328140.2">
        <property type="nucleotide sequence ID" value="XM_003328092.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHE6"/>
    <dbReference type="STRING" id="418459.E3KHE6"/>
    <dbReference type="GeneID" id="10532408"/>
    <dbReference type="KEGG" id="pgr:PGTG_09434"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09434"/>
    <dbReference type="eggNOG" id="ENOG502SCRC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009176_0_0_1"/>
    <dbReference type="InParanoid" id="E3KHE6"/>
    <dbReference type="OrthoDB" id="3207600at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046496">
        <property type="entry name" value="DUF6589"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20231">
        <property type="entry name" value="DUF6589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6589" evidence="2">
        <location>
            <begin position="419"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="877"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="14"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="898"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="941"/>
            <end position="954"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP83721.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="974" mass="109695" checksum="2CA26D62F28DB5C8" modified="2012-04-18" version="2">MSQEIPSMSQSKETPSMSQSQETPSMSQQTPSSSSTKYVSEEVKLKRVSDLLESLNWEPKSYIDHFLNATSDASIYKRRFWGCSSWTGTRRLLESIKRVVGKTDRGKLSWEDFILDEASMIICKQKPKTGYSPDGAYYSSKKLNRLFFTEEARLERAETIKESMPFLYNLLMRKLARPDTNDDSDDEEEKSDDGVDLAPLEESVPEPDEPHVPVLESDSDTLTELDPNAKIPIALSEDDVMEIEHNILRPSTDPKIRKHKQNEDMSRTICSMVTYGRNRRHNGFQLTNGLLFIACGVTERVNRYLNYIGITCCRRTAHLGLASLGKETEETLKTRSSDPNSKIFPPSICIDNLDFQQSIHTKSVGHMSTMFHGTWGYIHRLSSSFFNSLDHTELSLASLKQALKKSINLTVEPRHFASTFTSDEHFKSTLKSQLTSSFLRYIGSSSGKGPSLQDHPPPVRPIKADKPNLTMLKLMMASDNSSEGIGNVFKGLTEQTGLTPEQFSNQLQLVEGDLGTGMNILSLRELRIPASYSKTSLANLLAIPGAAHTMWNFSQSIFLHHWGDQTNRKDTGAWRVLKSLGIPADKPVTKMDFTLMISNMEKIHDADLIYCMLVVMGKECENLPTKLPTMSPLYIQGIVDQTYNRFLSGEAMETAVEKHQTKLINLLLRLRDFATVTEVNRASKAGNTGRLLFMWKQWAVMGQGLKKLVHYSRHLPQLILLIEVILPKSLAKVIENSLLLCPSGREAHFVATDFYLEVQNFWLKYFYNHSGIGTVIQRLRDVFSINIPILKDLMHTLNVQSGMNVIQQSHKNRITNLSINNFISFARQHNICSAEDKPDEFVPILISDIYEEGIATIKNNYANRKGGLDQLKPVYMFDPEIDNGDSDGTNKSNKSEDTNESEDTDESEDTNESEDTDESKDTDKSEDTGESEDTNESKDTDESEDTDDSNDSESGDSFFLTFSSSFLLGFGGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIN9</accession>
    <name>E3KIN9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_10542</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84164.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328583.2">
        <property type="nucleotide sequence ID" value="XM_003328535.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIN9"/>
    <dbReference type="GeneID" id="10545342"/>
    <dbReference type="KEGG" id="pgr:PGTG_10542"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10542"/>
    <dbReference type="eggNOG" id="KOG4266">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003559_1_2_1"/>
    <dbReference type="InParanoid" id="E3KIN9"/>
    <dbReference type="OrthoDB" id="3229693at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000036">
        <property type="entry name" value="Serin endopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000072">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010435">
        <property type="entry name" value="C5a/SBT2-like_Fn3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF66">
        <property type="entry name" value="SERIN ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06280">
        <property type="entry name" value="fn3_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7 8" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 8" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173611" evidence="9">
        <location>
            <begin position="18"/>
            <end position="902"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="10">
        <location>
            <begin position="146"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="domain" description="C5a peptidase/Subtilisin-like protease SBT2-like Fn3-like" evidence="11">
        <location>
            <begin position="605"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="6 7">
        <location>
            <position position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF06280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP84164.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="902" mass="97636" checksum="3753E6F3C0AC8CE1" modified="2012-04-18" version="2">MLICSLYFSIYFAVTLGLTNLTNAIQGKFSHSSERYLVSFNRNRSNSLEEFKAHLESKGINHEILIDLTNLVPEVFCGVSVQIFEEDLKHLESFAHVEQISRIGKVSRPRSLQERRLDGPTNSASLQYPPHIQTRISKLHEMGIAGQGVKVALIDTGIDCTHPAFGNGFGPGFKIGFGRSFVNDDGSPISPSESEAPRSPCTRCGAHGSSTAGILAASDTGYGFTGVAPSINLGMYRVVGCDEDVGAHDDVIIAAMLQAQKDAADIISISLGSGGGWGEGRELSRVANKLVQEKGVIMVASAGNSGHNGLFIGESISSSKNVIAVGSVDSQATVARNFKTSTGKNLTLYRPTAMDLPGEYLVYLTFNPTEGIMDACNKLPENTPNLKDYIVLIKNATNCLLFDQTKNAVAKGAKQILFYLGDSTTIPDLHTKMLNATISAIPMEDGNYIFDQAKKDPAGFKVSFPLTPYFYVRNLDGGLPSKYSTYGPSFDFESPQPAVAGVGGIVATTLPIQYGSYGLVAGTSMAAPQIAGIIALILSARGKGFTGPIMRSRLSTNAKVLNSPITSMGLDSVVHQGGGLIDAFCAVWTNTTISVPGLVLNDSISFKPNQGFTISNNGTSFVDYVLSHRPAVTLQTFSPDSKYGRPDLKPTPSNLSATAEIYPRQFRLSPGSSQSIEVKFLPPESLDPRWLAVYSGYIVMDSNVECESHNLPYYGVAGSLKEQSVIDRGPNQGNLTNYPYLTFKASDSGLHGNNTTLSVGKLTPLNGAFVWNLKEHNSTFLNFRLLFGSPLLRFDVLPGNTILSNDMDDMDVERSFRGTRLVGTIPVEGPNENICWGRSSYDHESRSWNGTIITQDKTTAQLLPNGNYRLLFRALRVTGTKENPDDYDHWISPEFKFINSQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJ35</accession>
    <name>E3KJ35_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_10030</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
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                <person name="Tuskan G.A."/>
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                <person name="Van de Peer Y."/>
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                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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                <person name="Martin F."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
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    <feature type="transmembrane region" description="Helical" evidence="9">
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            <begin position="1"/>
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            <begin position="1"/>
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        </location>
    </feature>
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        <source>
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        </source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
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    </evidence>
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</entry>
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        <submittedName>
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP84359.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="90" mass="10010" checksum="BB5F259B72CC8EE3" modified="2012-04-18" version="2">MADRELLGRQGYTPSTTEPLAEDKALIVCLTHNFVPGIVLLYDRLGMVKDVLRFKALGEIDRQKSWSPIKVIKILSKPTPSTTKSVSLGR</sequence>
</entry>
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    <accession>E3KJA3</accession>
    <name>E3KJA3_PUCGT</name>
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        <recommendedName>
            <fullName evidence="2">DUF7143 domain-containing protein</fullName>
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    <gene>
        <name evidence="3" type="ORF">PGTG_10098</name>
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    <organism evidence="3 4">
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
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                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178290">
        <property type="protein sequence ID" value="EFP84378.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003328749.2"/>
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    <dbReference type="AlphaFoldDB" id="E3KJA3"/>
    <dbReference type="GeneID" id="10545805"/>
    <dbReference type="KEGG" id="pgr:PGTG_10098"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10098"/>
    <dbReference type="HOGENOM" id="CLU_1455095_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJA3"/>
    <dbReference type="OrthoDB" id="2507284at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055567">
        <property type="entry name" value="DUF7143"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37592">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37592:SF1">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23631">
        <property type="entry name" value="DUF7143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173443" description="DUF7143 domain-containing protein" evidence="1">
        <location>
            <begin position="29"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7143" evidence="2">
        <location>
            <begin position="40"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP84378.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="190" mass="20722" checksum="9E2777BB96EB0931" modified="2012-04-18" version="2">MIETMLNLKNSRLILILVLQFLAPIVRTQSLCYIPNEAFDLPSDVKPPADVTCGSGTILGKIPDLIFSGRKFSEIDFTKSKLTPGGFALANFQFGGKNTQESLIISGKLYTAANAALRARGDRKILNRLKVVDFFITSQIDKSKGKEAKDSLLKHLGKVLKNCGKPKPKGNCNQGDVDKINAMVIEAKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KKR1</accession>
    <name>E3KKR1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10357</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178292">
        <property type="protein sequence ID" value="EFP84886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329305.1">
        <property type="nucleotide sequence ID" value="XM_003329257.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KKR1"/>
    <dbReference type="GeneID" id="10526860"/>
    <dbReference type="KEGG" id="pgr:PGTG_10357"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10357"/>
    <dbReference type="HOGENOM" id="CLU_1027244_0_0_1"/>
    <dbReference type="InParanoid" id="E3KKR1"/>
    <dbReference type="OrthoDB" id="10295017at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="52"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="63"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="145"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="271" mass="29150" checksum="FC89A7C44E90916E" modified="2011-01-11" version="1">MQPHTYQKHAGPGTRFIPPNLSNQSHYQPHVGSLAPAGAHVPSEYLAPAAGPQFNIANPQPSPAGAPSVPVSTNGAQGAGRHPDLSTPQLSTQVEVMSGGHRTNPSPTHGQLGLIGRSETGGIGPVRTTPQTSHLNHSPYRYEARSSAGDSASSFSQRSSLSDLSNDLIRPETLTYIRENYQQLQESLDNHKEFTRIGQPLFMIPEQERWAAAIALILHRDRVNISIPAATPGMETTQDTPNLKSLVSTNIRVILLDPKLDIYGQRVSASG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNA5</accession>
    <name>E3KNA5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptidyl-prolyl isomerase CWC27</fullName>
            <ecNumber evidence="3">5.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Peptidyl-prolyl isomerase cwc27</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11 12">Rotamase CWC27</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGTG_11070</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Involved in pre-mRNA splicing.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 17">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the cyclophilin-type PPIase family. CWC27 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="3"/>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EFP85741.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330160.2">
        <property type="nucleotide sequence ID" value="XM_003330112.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNA5"/>
    <dbReference type="FunCoup" id="E3KNA5">
        <property type="interactions" value="337"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KNA5"/>
    <dbReference type="GeneID" id="10544307"/>
    <dbReference type="KEGG" id="pgr:PGTG_11070"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11070"/>
    <dbReference type="eggNOG" id="KOG0885">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_14_4_1"/>
    <dbReference type="InParanoid" id="E3KNA5"/>
    <dbReference type="OrthoDB" id="442970at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01925">
        <property type="entry name" value="cyclophilin_CeCYP16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000007">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase CWC27 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF6">
        <property type="entry name" value="SPLICEOSOME-ASSOCIATED PROTEIN CWC27 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="14">
        <location>
            <begin position="23"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="311"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="447"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="519"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="324"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="359"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="447"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="519"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00055615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00071024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00082698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00083804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EFP85741.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="546" mass="61240" checksum="59F439DCC519DFE4" modified="2012-04-18" version="2">MALQAPNLFVTEPIPSGHVRFKTSLGPIEIELWPRETPLACRNFVQLCLEGYYEGLIFHRIVPGFIVQTGDPTGSGSGGESIYDEGTFNDEINARLKFTRRGLVAMANLGSPNTNTSQFFVTLDRTEELQDKHTIFGSVIGDTIYNVMKLGEVEIDKNERPIHPPIIKSVEVIDNPFPDIVPRITSAQRKEQAKARKEAKKEKQKEKMLAKHKALGQVESNFGWLLVYVENKGLLSFAEEERLTETSDGSKNRKLKSAHDLEASSQLSQAVLDQRPSLNQLNLPENFKSTLPATIPENPLKVHEALRAVVGRERSGPSESGKTNTKESPVDDSSNKKGKGKHEKKSKESNTELSAADQVKADIIKMQAELKKMSKRHDSDSDSEEDKTKGKTEKTQKRKVTHDGHSLLEEERKKYKARAGDKKTAGRGAASEAESILDSFRRKLREAKPLTKPAEEAERVDGYAGEVDPTQDGVLGDVDGDDDGWMAHTLQFRKDATADLHKVDEYAVIDPLRERKLTLDELEARKNEKRFDNRPQPRNSDRRKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQ90</accession>
    <name>E3KQ90_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GCM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12421</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178300">
        <property type="protein sequence ID" value="EFP86465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330884.1">
        <property type="nucleotide sequence ID" value="XM_003330836.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQ90"/>
    <dbReference type="GeneID" id="10540480"/>
    <dbReference type="KEGG" id="pgr:PGTG_12421"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12421"/>
    <dbReference type="eggNOG" id="ENOG502S7P8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005992_0_1_1"/>
    <dbReference type="InParanoid" id="E3KQ90"/>
    <dbReference type="OrthoDB" id="2506598at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1135"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1164" mass="131153" checksum="43F10C513D3892C2" modified="2011-01-11" version="1">MSFLHSSNEDNTSVDYSSESSYSEDIKTPVISNSTRLSRGKPTTTAETTLTIPSETNNKTFFLPTMNEKTFIDHGCTLDAEGYPLLPNGNTIFVKPSGIKVTNFGNVGFTKRTGSEYRSKGTWKLTRIYCLGSLACDLPQCRWAGAPPTGRVVMSEYLERYPHCRGSAGKCPGNIRHHTCKNTLIRVDEHVPTGWAVLRHKGQHEHPWPEVKKPDKLAKEALKNEILKNPKAGALSLKLGKPIAPRDPYDSVMSIHPALRNADRLRYYRKLMLREMNITPEKLGAGVGDKFINDMFMWNNVIPRRGLQITSASFLDGSEHFTFQTEWMAKRLLARDLDNRVYSGGLVSDVTYRFFENGYLLSTSMYVEEITRWVPVQLTWIRGLSAGYYRIHFATLFRQFLKPEITEVERDILVRNIVDFSLAQREGFIEAYGEVFGIFDRAQVLGKLQGCHQHYRAQVTRIRVNRNVIMAGDEVNFQQMCMDLIKEPQAGDPTHEEKIDALRRQFPKAKRWIDWWTMADVESMLFPSRRPQDEDAPEGVNALPKTSNAQESLHRLYYMISPGKTSLLVGMVQLFSFVKVLEEDFDAVMRGISIEYGSQRKGDTDVAQSIGWEKSRKRAPKAVPLGPGKKIRHEYANDGRAPDTTKALLPEIIKKKLGRPSGAPNINRNPFNSYPSYHASKKEHLKNRCWMAASVESLYAVFSPIWLQGSSGCGTSLFTTLVKHFTARATYELTEAGSIRSILSKAQCTLFHLVSAQHPRSFVAGKFASADFFLDVILDRKANPNRALKGLFEVEEVRQLTCKHHTKAAHELRRPSHVINIRRKAFESNELEFSDVASFLRQWTGSGLDGWSGLCCKLCNSSHPSSEMPTYGRASSDVEVAISTLVSTSSPKTPMLQERSCLAFIEDQPPAHLYFHLDVTPILDEDEQQSFMAVTDWPFTLYIRHVRYNLFARGFWDGSHYWCKVVRHVRGRTGVWLHNDRTNDGYAQLVSPIPSDLGGTAAHTSWLIYSRAWNDSELEFVNDKITQIKKDNPRATGQVPFAKLEELLEVSKDLDLGTVKPDTDNLVMDVFSNDNEVPNGNETTDDEDIKANEIKPAPSAAMRAPKLPKLKLILAKSGVIGVPAPVEAVKQIKSEIKKPKGSKKAVPQGGNRRSKRVEAKGGDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQB6</accession>
    <name>E3KQB6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF1279 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12447</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178300">
        <property type="protein sequence ID" value="EFP86491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330910.1">
        <property type="nucleotide sequence ID" value="XM_003330862.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQB6"/>
    <dbReference type="STRING" id="418459.E3KQB6"/>
    <dbReference type="GeneID" id="10540506"/>
    <dbReference type="KEGG" id="pgr:PGTG_12447"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12447"/>
    <dbReference type="HOGENOM" id="CLU_1111812_0_0_1"/>
    <dbReference type="InParanoid" id="E3KQB6"/>
    <dbReference type="OMA" id="REWMVEG"/>
    <dbReference type="OrthoDB" id="5580261at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018811">
        <property type="entry name" value="MRX11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28002">
        <property type="entry name" value="MIOREX COMPLEX COMPONENT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28002:SF1">
        <property type="entry name" value="MIOREX COMPLEX COMPONENT 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10306">
        <property type="entry name" value="FLILHELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="126"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="248" mass="27164" checksum="654A6E7D3E7694AF" modified="2011-01-11" version="1">MIGRRSLQRVQEWRTRTSRAQTQGLSRLARDGPAKRRWTSSSSSSSSSSTTRGGDSRMEKIFRTIQARYPHISANHLPSLTAAFVLLHELTALVPLLAIFGIVQTTGTGAKLVHFIRHSLPPPPSSDSDSSSDQEPASSVPQDRSDPTRHSAIAGSLTQALDESAAKVSKLAERYGYSSGQEEPEDDEACDEESRSMLENGVLASGLVAYLCVKILLPIRVSVSVYLTPSLARQIRRMAHWEPKRPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQR5</accession>
    <name>E3KQR5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_13022</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178301">
        <property type="protein sequence ID" value="EFP86640.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331059.2">
        <property type="nucleotide sequence ID" value="XM_003331011.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQR5"/>
    <dbReference type="GeneID" id="10540902"/>
    <dbReference type="KEGG" id="pgr:PGTG_13022"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13022"/>
    <dbReference type="HOGENOM" id="CLU_162854_0_0_1"/>
    <dbReference type="InParanoid" id="E3KQR5"/>
    <dbReference type="OrthoDB" id="2508290at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.160">
        <property type="entry name" value="HPT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036641">
        <property type="entry name" value="HPT_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47226">
        <property type="entry name" value="Histidine-containing phosphotransfer domain, HPT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP86640.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="125" mass="14820" checksum="38A048977F4EAF32" modified="2012-04-18" version="2">MGDIIDLDLFAELVRLDQQQPFLDEQISNYFYPSSKCIWAMMDELRSGDYRKLEQEAIELRILASSLAVVRVAQLCTFVENKCRSGLVDRDRLEIDTRLQVMELANQFAQDWLVKELYARRERRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3KS56</accession>
    <name>E3KS56_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="6">AlphaK I13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_13350</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EFP87131.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331550.2">
        <property type="nucleotide sequence ID" value="XM_003331502.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KS56"/>
    <dbReference type="GeneID" id="10542374"/>
    <dbReference type="KEGG" id="pgr:PGTG_13350"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13350"/>
    <dbReference type="HOGENOM" id="CLU_006433_0_0_1"/>
    <dbReference type="InParanoid" id="E3KS56"/>
    <dbReference type="OrthoDB" id="2500430at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.200.10">
        <property type="entry name" value="MHCK/EF2 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004166">
        <property type="entry name" value="a-kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02816">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51158">
        <property type="entry name" value="ALPHA_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Alpha-type protein kinase" evidence="5">
        <location>
            <begin position="309"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="25"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP87131.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="559" mass="63115" checksum="BF12038BE830905B" modified="2012-04-18" version="2">MKTKQKDALIRAKERLLKSIPEDQPSSTTHHSSHPVPSKNTEVEVDSSPPVDAYSFARARRVKPSPYPTRTSRNARLSKPKPAAPNKGAIPSSDPPQKVMVQCGFEIRVGGKFQPKLSEPRTPHWIETGNINSHNILLSELCQKYVGHDLGGYGSFSKNHFPFSKLGSKVAGVDDQASFIFHLEQNKFIDLIFNYDAYDKKCLKEEEEYSQSDSELETLDSIRIINPKKNPRLPLNTPTSYTIESNLDGNNNQVLDNASSLGIDQSGIYSGQTTPAFFSSLSSVSDRSQIQMLPNLAVQRCPPWVFNPIMDLSNFSWDRFGSTNLSTLFTDQNGLGWTEGIRLELDPNGIDMFRRLVHYKIHETEVISESEKGRVVKADLLDTPNIHSMIVKCSTKRQHPSLSHLPFPIYRARSYAAARQFLALFTNSVLTSSVAASDLKNLAQNLRIVQAFPMYPDPQHPERSYSDFPERENDVRASFEADPKKVFFVEEYITGTWKTLLDPDTFNTRLNDYSPAIDHLMEAFQHWTYNYTKGQIVVTNLKGVAPVMSKPKIVDLNRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3KS91</accession>
    <name>E3KS91_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_13385</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EFP87166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331585.1">
        <property type="nucleotide sequence ID" value="XM_003331537.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KS91"/>
    <dbReference type="STRING" id="418459.E3KS91"/>
    <dbReference type="GeneID" id="10537396"/>
    <dbReference type="KEGG" id="pgr:PGTG_13385"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13385"/>
    <dbReference type="HOGENOM" id="CLU_164927_0_0_1"/>
    <dbReference type="InParanoid" id="E3KS91"/>
    <dbReference type="OMA" id="VPTNEIC"/>
    <dbReference type="OrthoDB" id="2503647at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052925">
        <property type="entry name" value="Phage_Integrase-like_Recomb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34605:SF3">
        <property type="entry name" value="P CELL-TYPE AGGLUTINATION PROTEIN MAP4-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34605">
        <property type="entry name" value="PHAGE_INTEGRASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP87166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="13019" checksum="792516421522185D" modified="2011-01-11" version="1">MRRLADAKKNGVREGDGHNSLFSFTDPEGGRFHITKPMATRALDKIWKACGYAGVSGHSFRVGGASLLKALGIPITQICLRGRWASDSYKLYLREFTEEDIESTNGLLRQLEEAWSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KT12</accession>
    <name>E3KT12_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13626</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178306">
        <property type="protein sequence ID" value="EFP87398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331817.1">
        <property type="nucleotide sequence ID" value="XM_003331769.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KT12"/>
    <dbReference type="GeneID" id="10538127"/>
    <dbReference type="KEGG" id="pgr:PGTG_13626"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13626"/>
    <dbReference type="HOGENOM" id="CLU_2980161_0_0_1"/>
    <dbReference type="InParanoid" id="E3KT12"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="58" mass="6487" checksum="ADFDCE2EBFE81D31" modified="2011-01-11" version="1">MLAHLGDSWDDLAVSNDNDGPVGPQLNDEDDDTVHREEIQNKCLYFNYLNGTLPIPSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>E3KUR0</accession>
    <name>E3KUR0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="9">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_13814</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Responds to activation by environmental stress by phosphorylating downstream targets.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="9"/>
    <dbReference type="EMBL" id="DS178310">
        <property type="protein sequence ID" value="EFP88010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332429.1">
        <property type="nucleotide sequence ID" value="XM_003332381.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KUR0"/>
    <dbReference type="FunCoup" id="E3KUR0">
        <property type="interactions" value="603"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KUR0"/>
    <dbReference type="GeneID" id="10535069"/>
    <dbReference type="KEGG" id="pgr:PGTG_13814"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13814"/>
    <dbReference type="eggNOG" id="KOG0660">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_181_1_1"/>
    <dbReference type="InParanoid" id="E3KUR0"/>
    <dbReference type="OMA" id="CYFLYQM"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07849">
        <property type="entry name" value="STKc_ERK1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000040">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000073">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="75"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="25"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00055111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP88010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="416" mass="46764" checksum="28911F9E0C642EFE" modified="2011-01-11" version="1">MAAAVALPSSATGGATLPGSGPTVANTSTNNNNNPAGNASSTTTTSNTTAPAQKPASSRTPAEPRRVRFNVGSKYHVMDVIGEGAYGVVCSAVHRPTGQKVAIKKITPFDHSMFCLRTLRELKLLKYFQEQNVSENIISIVDIIRPPSIEAFKEVYLIQELMETDMHRVIRTQDLSDDHCQYFIYQTLRALKALHSADVIHRDLKPSNLLLNANCDLKVCDFGLARSVRTAEQETGFMTEYVATRWYRAPEIMLTFKQYTKAIDVWSVGCILAEMLSGRPLFPGRDYHHQLTLILDVLGTPSLDEFYAINSRRSRDYIRALPFRKKRPFNVLYPNASALAIDFLTKTLTFDPKKRLSVEQALQHPYLEAYHDPDDEPVAPPLDEDFFAFDRQKDEISKEELKQLLFDEIASFHSTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3KUY6</accession>
    <name>E3KUY6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_12533</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178311">
        <property type="protein sequence ID" value="EFP88086.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332505.2">
        <property type="nucleotide sequence ID" value="XM_003332457.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KUY6"/>
    <dbReference type="STRING" id="418459.E3KUY6"/>
    <dbReference type="GeneID" id="10535227"/>
    <dbReference type="KEGG" id="pgr:PGTG_12533"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12533"/>
    <dbReference type="eggNOG" id="KOG1849">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_612713_0_0_1"/>
    <dbReference type="InParanoid" id="E3KUY6"/>
    <dbReference type="OrthoDB" id="6880011at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004096">
        <property type="term" value="F:catalase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1370.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.180.10">
        <property type="entry name" value="Catalase core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018028">
        <property type="entry name" value="Catalase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011614">
        <property type="entry name" value="Catalase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002226">
        <property type="entry name" value="Catalase_haem_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010582">
        <property type="entry name" value="Catalase_immune_responsive"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020835">
        <property type="entry name" value="Catalase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11465">
        <property type="entry name" value="CATALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11465:SF62">
        <property type="entry name" value="CATALASE T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00199">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06628">
        <property type="entry name" value="Catalase-rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03568">
        <property type="entry name" value="Separin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56634">
        <property type="entry name" value="Heme-dependent catalase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00437">
        <property type="entry name" value="CATALASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51402">
        <property type="entry name" value="CATALASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0349">Heme</keyword>
    <keyword evidence="6" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="1" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173740" evidence="7">
        <location>
            <begin position="24"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="domain" description="Catalase core" evidence="8">
        <location>
            <begin position="30"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Catalase immune-responsive" evidence="9">
        <location>
            <begin position="368"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP88086.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="447" mass="50055" checksum="BEF5262F2DCBE9CB" modified="2012-04-18" version="2">MSMIIIVVDLVPLLLETLQNLLALLPRSNRPSADPVLQSRLFSYADAHIYRLGINYTQLPVNKSVSPVANSQQSRDGRWVSVADTRHPLADTRQRLRMAPFPQNPGGYPGIPKDTRGRKLGLDDVLQKFRVDPLLGVLWEATMEEALTKRTIGCANIPNLHEAIHVLITLKFINSILQQEQKNVAGEVAARLVVGTGDDTSWPSEVEVKESLLAGDEMQIASISGANLSRWQDVASHHRNDIRLPATYCPLQDLPTGLHVVLIHLAFEEDSLFIIQLNKMCNPLGVKLLLDWFNWREGEDELFTLKVALDELQAIVSKSNAATQRARHVTGRQDKISWWQERKDLDTRMRNLVERIESDWLGACKTLDFKPACALWSKVFDDGAKERFVKNVSGHLGNVSLDCIKTDQVAIFLAVNKDLGNRVAKAISLKDTPEPYKPEPAAQATNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KV57</accession>
    <name>E3KV57_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14364</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178312">
        <property type="protein sequence ID" value="EFP88280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332699.1">
        <property type="nucleotide sequence ID" value="XM_003332651.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KV57"/>
    <dbReference type="STRING" id="418459.E3KV57"/>
    <dbReference type="GeneID" id="10535671"/>
    <dbReference type="KEGG" id="pgr:PGTG_14364"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14364"/>
    <dbReference type="HOGENOM" id="CLU_052858_0_0_1"/>
    <dbReference type="InParanoid" id="E3KV57"/>
    <dbReference type="OMA" id="FEDIMTI"/>
    <dbReference type="OrthoDB" id="2500854at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="428" mass="48020" checksum="8279B5BB80260758" modified="2011-01-11" version="1">MVNTQANPISGLIDSFASLGNERCAHPIRCHEAMVSNELARLNNAYSSLSDGIHNRPDPLEIFNQVEMRTELLDQLRSKRFPSLRRQVIALSTALISPTNSQNDPLSKLTLVLKVLSKLDVTLGKIKFAIACINPSLDPEEVTHDKDFKKAKQLICCRLGIIVDLVTGYVCELLRVSCRFIEDSGHAFAIDQPQKKAKVLTITDTFSVSLDRALQFMNKSESDFMKDAWRSHMESIDGSLKKFLEFLHRLAPRSKNGRRSAGNPRAAHAGARRGNARSNITQPSHQPQTTKSAIALIKLTRLFLAKLSKMSTDKEHFRVVTELNSRELSIFAGLTTMVSGCIEKLVEGLCGVIGGDEFEDIMTVHGSISRLQTAPKMITTIVNHIFVPVVHQADQPSHKMYYKAWFYQWNRLHQLAAENFLDAFMFNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVM4</accession>
    <name>E3KVM4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vesicle trafficking between the ER and Golgi</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_14448</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178313">
        <property type="protein sequence ID" value="EFP88364.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332783.2">
        <property type="nucleotide sequence ID" value="XM_003332735.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KVM4"/>
    <dbReference type="FunCoup" id="E3KVM4">
        <property type="interactions" value="826"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KVM4"/>
    <dbReference type="GeneID" id="10535837"/>
    <dbReference type="KEGG" id="pgr:PGTG_14448"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14448"/>
    <dbReference type="eggNOG" id="KOG1301">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016216_3_1_1"/>
    <dbReference type="InParanoid" id="E3KVM4"/>
    <dbReference type="OrthoDB" id="10251230at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP88364.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="693" mass="76284" checksum="3A1280AA567CC5C8" modified="2012-04-18" version="2">MNHLNHQRNSLRDHQLQSLNSLLSFNNPQQQQQQQQQQQQQHQLKTAATTTNLPTWKVLVLDKQSQDVLATTLRVQDLRELGVTLHMQLNADRPPLPDVPAVYLVSPSRESIKRIAIDLDKGLYESFYLNFTSTLSRPLLEELASLVVESGSDSLIEQVYDQFLDFIVLDPNLFCLSSPTGTTNLNPSNSLKRTTYEALNDPKASEQEIEGIADQIAKSLFSVVATMGQLPIIRCPRGNAAEMVARKLDSKLRDYVLSTRSNSVFTSEGSRPVLVILDRNLDLVPMISHSWTYQALVTDVLEMKLNRVTVEAPESGKLQKKTYDLDSKDFFWAKNSCKPFPEVAEEIDTELTKYKSDAAEITRSTGIGDINDVSQIDLTSNAAHLKAAITALPELTARKTTLDTHMNIATALLQGIKNRGLDTLFQMEESITKQTRQALLELLKDSSKDPTDKLRLMLVYYLSNQDVSKDELLEYERLLKESGLEKFGTWEYVKKIREISRMTNSMALASASVAAPPASTGTGGELFRGFSSISNRLTDRLKEGGLGGGIDNILSGVKNFLPGRKDFIITRLIEALMDPAAASNQALADIDDYLTLDPRSSRNFSAPSHQNKKANRITFAEAIVFVVGGGGYVEASNLQEFAARSSNYGGGGGGAGSTGPGGIGSGSKKITYGATEILSPLEFISCLHNLSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVY9</accession>
    <name>E3KVY9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BTB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_14042</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178314">
        <property type="protein sequence ID" value="EFP88464.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332883.2">
        <property type="nucleotide sequence ID" value="XM_003332835.2"/>
    </dbReference>
    <dbReference type="FunCoup" id="E3KVY9">
        <property type="interactions" value="101"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KVY9"/>
    <dbReference type="GeneID" id="10540767"/>
    <dbReference type="KEGG" id="pgr:PGTG_14042"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14042"/>
    <dbReference type="eggNOG" id="KOG0783">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002285_1_0_1"/>
    <dbReference type="InParanoid" id="E3KVY9"/>
    <dbReference type="OrthoDB" id="1893551at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000210">
        <property type="entry name" value="BTB/POZ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000408">
        <property type="entry name" value="Reg_chr_condens"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051625">
        <property type="entry name" value="Signaling_Regulatory_Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872">
        <property type="entry name" value="BTK-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22872:SF2">
        <property type="entry name" value="INHIBITOR OF BRUTON TYROSINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00415">
        <property type="entry name" value="RCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50097">
        <property type="entry name" value="BTB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50012">
        <property type="entry name" value="RCC1_3"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="2">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="161"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="231"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="301"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="repeat" description="RCC1" evidence="3">
        <location>
            <begin position="361"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="domain" description="BTB" evidence="5">
        <location>
            <begin position="934"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1196"/>
            <end position="1240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1301"/>
            <end position="1407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1516"/>
            <end position="1548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1605"/>
            <end position="1796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1214"/>
            <end position="1224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1301"/>
            <end position="1318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1335"/>
            <end position="1351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1352"/>
            <end position="1365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1389"/>
            <end position="1407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1523"/>
            <end position="1536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1537"/>
            <end position="1548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1621"/>
            <end position="1648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1708"/>
            <end position="1769"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP88464.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1796" mass="197569" checksum="B4DC49D89340E56B" modified="2012-04-18" version="2">MKPNQSNNGLILDINIHDVYQSGDINCLRRFLNTIETTCKKTSLISSTINRKDQSGRTLLHKLAASRNESDSIEWLELLAQNHLLQLNIPDLESGWTPLHRALYHGNLRFAKVLIDQLECDLNTKDHEGLSAFDLYHSTIDGSINWISSKPSQAENQQPHTDLFTWGSNRNFVLGIHDDGDRHHPERIHLKRSISSSSQLDPISSSNHFISNPLNFVAIGRLHTALITSEGELRVCGFGSGGRLGLSTSSKSNAQDQRPTAGISAETQFTFAPVKRGGLAQQKVKFVALGQDHTVVITNPGQVYTFGMNRFGQLGYGPESAGASIENMIQVEPKRVLGLLKKVDVIGAAASRWHTAVFSSDSLFTWGANRGQLGYHNPHSLNSLASIQITPRKVAIAHTSIIQLSCTSYETAYLCENSSEVYVLKAEVTIRVVFPFDRFPSDIQVSGMPSTHQGHTPATRILQLDSSDQTLAAVSSMGDVYIVDLENPNPLENPINYVNMSSSPHRSNISTTPTGPAFKPRRIWCSTRSTTAAKHVAVGLDGDVIITTMSGHVYISYRRSAVNAPLKSLDTRSTEPSVISHPKKNYKFQRVQYLQRVSRVAANPTGSYAAIRMDVQLREIQQSLPEEKITLASRLASMLPHLKKYASSVPELHTVPSAILTSKMPSDLSTSGHFRSGSTSPSSEVHLDADEDVIEEEEDREDLVWDSRVGSALFGLSLNWSDDHPTLDSGDIFLVANSGEKILVHRFILCSRSLILDQILNNLTHVDGISYHPGETGEDDDDAEQGSLRLPTLRFDQHMLLTLLLFTHYIYTDTFPTIWDNRVWPQIEHAHQEFLSNTRSSGKPKHAIIHQLNEAKEGLKSLARSLGFPALELSLMRLGKATPKPLLSNHFRMILPTLGLSNSSSEAHDDSQANPQVDPDHDFIPNFQSSNLNPDMELELKDGEILTCHSILMRAQCPFFRLMYDQEVWVTGRRRAKVTGNSNLIRIDMGHLSKKVMQVVLRHIYTDEADDLFLAKDFLSLNDYIDFVFDVMSVANELMMDKLKYICSVVLRRCVNLSNVTAIASEAEFYRADSLKETCMNYMIYNLEALFEDKGLIKIPNDLLGSISSYLKRVQTDKFPISRSTRIIEELMADNSQFLIDLDLPKSKLVLTTRHWKSFHKSLTHQSSQALPDLDITFDKRISRGSVAIDPHQLITGSSFQPSFSPPPDPVGRNQATHPDTSAIDSGPGMNGTPSRTLESVHETMDDDAPFVMDELDGAVHSTTKAVGKMKLGHSSAPHDSSAWAAPVKKVTPIDLRMVTSSSKQRGSFGPSNSQLDTLPSLRLPSDLTPNQKLSQREKRRQSAEASKSKPDSSSTSAAQSIPSKAPAWRSMSGGSSDRKIAGPGGQGLPSLMSNPSLSCSPSSSQPPVGLIKNVQATGPLVGRVQGAVVPRNPEPNDPASAIGASVITPTRAVNGIGASNTFRRALAGQDTPWTNYVSTSTQFVFPGTSASPLDSSSNPVLGSQYENKFEEHTLTLGGSHGIENTSGTNQTMARKNTSNSSQTTTTNFSTIQELQAKESWALYGPGKQKPKLAEIQEEENRRLMEKKQEEQFLKWFEEESLRLQQQQQLPQANSRPKKSAASTRNKTGSSSNRPTHPTSSGNSTITPLPQRPHPKDKKVTQNSDKSALPEHPHKASNKPPPDLSSTSAVHHPELPNPSPKGKKKAKANNNKAMLSNSKQFSTSSTPTSTATTTVALTPSDKKPSSMTSVPSSSSTTAATNRGSNNTQLSHHHHHHHQDEHLPTLHHQNSSRVKAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZL7</accession>
    <name>E3KZL7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15398</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178325">
        <property type="protein sequence ID" value="EFP89742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334161.1">
        <property type="nucleotide sequence ID" value="XM_003334113.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZL7"/>
    <dbReference type="GeneID" id="10545391"/>
    <dbReference type="KEGG" id="pgr:PGTG_15398"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15398"/>
    <dbReference type="HOGENOM" id="CLU_407171_0_0_1"/>
    <dbReference type="InParanoid" id="E3KZL7"/>
    <dbReference type="OrthoDB" id="2498185at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="637"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="681"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="340"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="637"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="693"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="712"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="768" mass="88005" checksum="47F6150B994CB72E" modified="2011-01-11" version="1">MTEPKTASDQESSIDRPGPVSSSSAGRLENLLGSPYWPLGSSFLKLLNFPPAFRTISCLSGSASTPLAIETNPKDDCPKSPKEDQPVPHEPDQMEVDLDGSPIESDDGCSQRGDERLVAHQDEDEDDDHTLEELLVKPTSTENRRHPTSARKPSLSDRLTEPSTWKHGSDRHRSSREYDYRPTGRLPFASIPRNTNFHIKGLASLKRTGQQVSEPFRTSSNLIECNTKLHPWGSKRPNPTSSGAQPSYHYNKRSRLSEPSQKPSESKKWVRDRYQESDEEYDHRRVSEEINSQRYHWPKSHQKTEYYKHKRLDSPVSHASEPQDRASSTAGWKKYDDKTGFTNPKSQNTSQDFHDEVEDRERRWMQEFKEYEVSRTAHTRSVSPFPSSHRRSPSRHSSESDSDYYALTQIPPELKEALHRAVFSWINRAIAEALCRLGLWLEEKKIWSPTEWGSIRVDQLEKVVNMTYDRGLSYLMWKCNTVEFMDLLQKPIQSGIFFIHKDPQNDAWYIRFGRDFTPIGLLYRPIVKGYRKHLTDSKSQLPIQVGKFVRQYIMSCPNEEDLLYLFKDDKDPMGSLRRAEKDGIIELTDQPPSHGSIGNRDGLYGNRTWYNKTAVENKYIQLKPYWLSNKDPVDQPLLSSPTTTVQTCDTAPNDDDDLPSNSASKNIKQKVSNLMENHQGDNQLPAIDRRNSDNTSLSANTTSTTTDTGNEQLDHAPVGHERERTRSHRSAAEIETVKPKSSHSPNVDDLIQSVVDQLNQHTSRQKRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZU5</accession>
    <name>E3KZU5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_15782</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178326">
        <property type="protein sequence ID" value="EFP89826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334245.1">
        <property type="nucleotide sequence ID" value="XM_003334197.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZU5"/>
    <dbReference type="STRING" id="418459.E3KZU5"/>
    <dbReference type="ESTHER" id="pucgt-e3kzu5">
        <property type="family name" value="Canar_LipB"/>
    </dbReference>
    <dbReference type="GeneID" id="10545557"/>
    <dbReference type="KEGG" id="pgr:PGTG_15782"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15782"/>
    <dbReference type="eggNOG" id="ENOG502QSW8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029537_3_0_1"/>
    <dbReference type="InParanoid" id="E3KZU5"/>
    <dbReference type="OMA" id="ASHITML"/>
    <dbReference type="OrthoDB" id="4605274at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053228">
        <property type="entry name" value="Stereospecific_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37574">
        <property type="entry name" value="LIPASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37574:SF1">
        <property type="entry name" value="LIPASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172961" evidence="2">
        <location>
            <begin position="19"/>
            <end position="474"/>
        </location>
    </feature>
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            <begin position="39"/>
            <end position="76"/>
        </location>
    </feature>
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        <location>
            <begin position="39"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP89826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="474" mass="49753" checksum="2B9DE41C05505D72" modified="2011-01-11" version="1">MIALTPVWLFFLVLHAGATEPAERQKHNGDQTDLSKRGLLSNLLSPNPTSSPSRGGITGLLGGIPNRGFQGGPESIPGGLLDGVTSLLPLGPGNGLNDLLNGIPKYNPAAATAGPRPASSYGDAPWTQSEMTYRQQISCPGGINGQRGVVLLLPPTAASGQQVWPRSPYAQLPRYGFSICWVDNLTKGTGDAQLTAEFVAYAIKYLATVSRRPINVISYSQGGMNMQWALTFWPSIRRMVMGFVALASPFHGTYASSIVCQVDELIGGCLPALFQLQTNSQFMRALNAPVMGSGAVALVPTTNIYTLEDEIVLPQVGGRPISYLNGASNMALQEVCGATHVADHFLIVGDYGAFGIALTALMSGRPANPAAVDKSFCNQLAGLGGQLGSLGNDLKYTFGSIVGNTGDRIHSLLKTATTLSVTAEPPLQMYVCQRGFATGCTGNGFSGPEARAPILSHLPQTLDAVGSLLGGLRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3L0Q5</accession>
    <name>E3L0Q5_PUCGT</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15957</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <dbReference type="KEGG" id="pgr:PGTG_15957"/>
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    <dbReference type="HOGENOM" id="CLU_2387258_0_0_1"/>
    <dbReference type="InParanoid" id="E3L0Q5"/>
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    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90109.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="94" mass="10251" checksum="11A882CA9663EB25" modified="2012-04-18" version="2">MARRESPSCQDLAVLMTGRNPSRPSILYKSQRPDGIPPSHQDCTSLKDQTESLPATMGSHTKRPTNVGVMALKTARQTCSVSATTLCYTVLQPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3L161</accession>
    <name>E3L161_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="8">AlphaK A2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_16361</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178331">
        <property type="protein sequence ID" value="EFP90335.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334754.2">
        <property type="nucleotide sequence ID" value="XM_003334706.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L161"/>
    <dbReference type="GeneID" id="10530229"/>
    <dbReference type="KEGG" id="pgr:PGTG_16361"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16361"/>
    <dbReference type="HOGENOM" id="CLU_020761_2_0_1"/>
    <dbReference type="InParanoid" id="E3L161"/>
    <dbReference type="OrthoDB" id="301415at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031037">
        <property type="term" value="P:myosin II filament disassembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04515">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.200.10">
        <property type="entry name" value="MHCK/EF2 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004166">
        <property type="entry name" value="a-kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051852">
        <property type="entry name" value="Alpha-type_PK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45992:SF2">
        <property type="entry name" value="EUKARYOTIC ELONGATION FACTOR 2 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45992">
        <property type="entry name" value="EUKARYOTIC ELONGATION FACTOR 2 KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02816">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00811">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51158">
        <property type="entry name" value="ALPHA_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Alpha-type protein kinase" evidence="7">
        <location>
            <begin position="310"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="89"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="223"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="103"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP90335.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="636" mass="69819" checksum="ABE0855F86A5C06C" modified="2012-04-18" version="2">MVTCLKCTRFVTQTEFGWCCECLNFVGLMPGNMAPQLPSQPPNHYNIIPSYPPAAAPTPSIFQALPANPTTPAANLAQCAGSNAHIRNIRKSKRTKNPAPTPYSRNPKPSVSATLTESPMRMFKCGISFFQDKKQKKTGIMSYMRQIDTSLPNLYETLQHQLWQLFLPELLRKELVEGLPKSPIGRTSLSHNKSVLDPQTLLLLVKQSTIKKPVQIDLIYEDNGEDTDTIPETETPAATERSLRSQQSSATFRIPSPLESQIVPRQTSHITSRRHNEDQASTPTAVAARPTKNSWALGGIAGTVPARGSASLSTHMNYLGRPLATTTPLTVKVDHNTLVGQGSMRRAFSAEVKTEGRNGGPPRITHWVAKVRYHNEYPDIKLHATNARMYEAAGHLLQAYQAVINRCSSNLLTGVLREKAKAFKLVRHCVIFIGEEHLPAQVYFLEACLQGEYVKYLSNVNFSVTGRQPGMDLDNLSIMNAFTHWSYVISNGASLVCDLQGVGSTITDPQIIDRDYVRWADGNNASKGIQNFLENHICNDVCRALKLGPPSDIVPEVTNPTPLQRSVQSHVAQGLGGPSQAAGFPTGSRAMTEIRSNSGICANPAPSHSSIGNLPRSEFLTFYCSKLVNSVKITLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3L2Y5</accession>
    <name>E3L2Y5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ribonuclease T2-like</fullName>
            <ecNumber evidence="4">4.6.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PGTG_17182</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Rnase which modulates cell survival under stress conditions. Released from the vacuole to the cytoplasm during stress to promote tRNA and rRNA cleavage and to activate separately a downstream pathway that promotes cell death. Involved in cell size, vacuolar morphology and growth at high temperatures and high salt concentration.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Vacuole lumen</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 16">Belongs to the RNase T2 family.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.19" evidence="4"/>
    <dbReference type="EMBL" id="DS178339">
        <property type="protein sequence ID" value="EFP90910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335329.1">
        <property type="nucleotide sequence ID" value="XM_003335281.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L2Y5"/>
    <dbReference type="STRING" id="418459.E3L2Y5"/>
    <dbReference type="GeneID" id="10528320"/>
    <dbReference type="KEGG" id="pgr:PGTG_17182"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17182"/>
    <dbReference type="eggNOG" id="KOG1642">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037966_0_1_1"/>
    <dbReference type="InParanoid" id="E3L2Y5"/>
    <dbReference type="OMA" id="HESLWIH"/>
    <dbReference type="OrthoDB" id="435754at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005775">
        <property type="term" value="C:vacuolar lumen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033897">
        <property type="term" value="F:ribonuclease T2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01061">
        <property type="entry name" value="RNase_T2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.730.10:FF:000004">
        <property type="entry name" value="Ribonuclease T2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.730.10">
        <property type="entry name" value="Ribonuclease T2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033697">
        <property type="entry name" value="Ribonuclease_T2_eukaryotic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001568">
        <property type="entry name" value="RNase_T2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036430">
        <property type="entry name" value="RNase_T2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018188">
        <property type="entry name" value="RNase_T2_His_AS_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033130">
        <property type="entry name" value="RNase_T2_His_AS_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057328">
        <property type="entry name" value="RNaseT2L_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240">
        <property type="entry name" value="RIBONUCLEASE T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11240:SF22">
        <property type="entry name" value="RIBONUCLEASE T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00445">
        <property type="entry name" value="Ribonuclease_T2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25488">
        <property type="entry name" value="RNaseT2L_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55895">
        <property type="entry name" value="Ribonuclease Rh-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00530">
        <property type="entry name" value="RNASE_T2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00531">
        <property type="entry name" value="RNASE_T2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 18" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0926">Vacuole</keyword>
    <feature type="signal peptide" evidence="18">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172411" description="Ribonuclease T2-like" evidence="18">
        <location>
            <begin position="21"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="domain" description="RNase T2-like C-terminal" evidence="19">
        <location>
            <begin position="306"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="279"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="active site" evidence="15">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <feature type="active site" evidence="15">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="active site" evidence="15">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00071169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR633697-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF25488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="EFP90910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="424" mass="46676" checksum="476777C431E6DF52" modified="2011-01-11" version="1">MVSSAILLLPAILIAINTHSFSVNTQQLIFTNNNQLNTQDHTCPANPPTSCHLSSGDGKEPVNSCCTNTPGGQILLTQFWDSHPATGPSDSWTIHGLWPDNCDGTYEASCDSGRQYHNITQILEQGGGQEALSVMKEFWKDQHGNDETFWEHEWAKHGTCISTLEPHCMGSSYKPQSEVVTFFNRTVDLFKSLPTYEWLKAANIVPSATTTYNLDQLQEVAKQYTGQEAVWNCRGHVLNEVWWHFNTVGTVADGKFVHASPVGPRSTCPYKGIQYLPKGHSGGGGGGGGDHRPKHPGHQPGNPPSDRKHFIYVVDEHGQRNGCLISNGNWLSRATCASFSVSVGHDDKALLEIRTRRGACAFEDSGFRCAKGLKSEGFEKNGDRLVYEGQEVFYSDKLPEGTTQVPILKSKSKNSIYLEIADAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3L351</accession>
    <name>E3L351_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17248</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP90976.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    <dbReference type="STRING" id="418459.E3L351"/>
    <dbReference type="GeneID" id="10528391"/>
    <dbReference type="KEGG" id="pgr:PGTG_17248"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17248"/>
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    <dbReference type="OrthoDB" id="2502185at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
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    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    </dbReference>
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        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
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        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
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        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="HYDROLASE, ALPHA/BETA FOLD FAMILY PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="alpha/beta-Hydrolases"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="17"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90976.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="205" mass="23314" checksum="2C8258CBB797CCCD" modified="2012-04-18" version="2">MAQDTMTLLKWLGWEQDRSIHLFGVSLGGMIAQELCLIIPERFKSTRSFSAVFQTSSRVATGDQALDLYLELLFPDGHFELETEEGKRYKSNLREKLRNCHHLPREQPYLAFGGHFYAAMMHHCSYGKLEKIATDLHPAKILVITGDSDELVLPKRSPELHEHLPGSELIVVRNAGHALPYQISDEFNAIMDRVIKEGNLAFSKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3Y0</accession>
    <name>E3L3Y0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17112</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
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            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178344">
        <property type="protein sequence ID" value="EFP91255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003335626.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L3Y0"/>
    <dbReference type="GeneID" id="10529001"/>
    <dbReference type="KEGG" id="pgr:PGTG_17112"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17112"/>
    <dbReference type="HOGENOM" id="CLU_077472_0_0_1"/>
    <dbReference type="InParanoid" id="E3L3Y0"/>
    <dbReference type="OrthoDB" id="2506424at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
    <dbReference type="GO" id="GO:0003676">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
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        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="26968" checksum="A36DEAE8E72E4C77" modified="2011-01-11" version="1">MPHPLPTIPPNSYINRFKRGHTIIRAWPGAVKPFLNLSLMQIATNVNQALSSIDARIYNTPVQVQAITRFPSGDVKFITKNRTIARWLLEHKHTWTHLADPNFTTPRATFTVMIHSVPTEFDTKNNNHLATLCTQNDIPCDMIEKTRWLGQPQKNGKKHGTLLINVKDKQLARDIKHGCLIIDGIPLKASKYTPGPPQCFNCLEFGHPAYFCKTPPLCARCGGKHNSKDCHIDNPSGACF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3L553</accession>
    <name>E3L553_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_17534</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP91678.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336097.2">
        <property type="nucleotide sequence ID" value="XM_003336049.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L553"/>
    <dbReference type="GeneID" id="10531617"/>
    <dbReference type="KEGG" id="pgr:PGTG_17534"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17534"/>
    <dbReference type="HOGENOM" id="CLU_2777172_0_0_1"/>
    <dbReference type="InParanoid" id="E3L553"/>
    <dbReference type="OrthoDB" id="2501436at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP91678.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="69" mass="7742" checksum="1244932A6ABA24C0" modified="2012-04-18" version="2">MQYGFGPASQVTREGSSTCTVSRLPSKSIIDVCCENPVGLRNPRPFESVFTVSENDYKNTFKCREPTDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3L5S7</accession>
    <name>E3L5S7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17929</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178354">
        <property type="protein sequence ID" value="EFP91902.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336321.2">
        <property type="nucleotide sequence ID" value="XM_003336273.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L5S7"/>
    <dbReference type="GeneID" id="10531849"/>
    <dbReference type="KEGG" id="pgr:PGTG_17929"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17929"/>
    <dbReference type="HOGENOM" id="CLU_109039_0_0_1"/>
    <dbReference type="InParanoid" id="E3L5S7"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91902.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="153" mass="16573" checksum="76CE895C649CBBAC" modified="2012-04-18" version="2">MLLSFVLPFLGSSSDRSMPRSSPMSSPACNHASIASAKLFVSTPSPTVCRAVYRCNLPTSPYLIEAKIPQRPRRLVTVPPPAPPSSTPLDSSSFSSDNAIALWSCHQLSSSDGEIRIRQTPFFSPPFVRQAQQHDASLFGRSSSFLEAPPLDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3L5X3</accession>
    <name>E3L5X3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phosphatidylinositol N-acetylglucosaminyltransferase subunit C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_18399</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PIGC family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178355">
        <property type="protein sequence ID" value="EFP91948.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336367.2">
        <property type="nucleotide sequence ID" value="XM_003336319.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L5X3"/>
    <dbReference type="FunCoup" id="E3L5X3">
        <property type="interactions" value="288"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L5X3"/>
    <dbReference type="GeneID" id="10531900"/>
    <dbReference type="KEGG" id="pgr:PGTG_18399"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18399"/>
    <dbReference type="eggNOG" id="KOG3059">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024002_2_0_1"/>
    <dbReference type="InParanoid" id="E3L5X3"/>
    <dbReference type="OrthoDB" id="196709at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000506">
        <property type="term" value="C:glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009450">
        <property type="entry name" value="Plno_GlcNAc_GPI2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12982">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL GLYCAN, CLASS C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12982:SF0">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06432">
        <property type="entry name" value="GPI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016104">
        <property type="entry name" value="GPI2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="55"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="93"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="121"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="181"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="211"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="268"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP91948.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="307" mass="34380" checksum="0D8A2F36FB916FE0" modified="2012-04-18" version="2">MMASPHHSTTSSALPQKPKFKRVLWCKQPYPDNYQDLTFLNHLQRNRTAHRTIDGYPTLVICALPLCAHLSSVMIFVGLFVYLYKGYILAQELIWYTISLGLVGYLVWERSKSDRRLARPTVLRSTGILIFLLLLISPVLKTLTLSTDSDSIWALSSVLFTINILLADYRDGRESLIEGSLDVSFPSALSINAAISASVVLGSRLPSNADVFALVLFAVEWFALFPLMRRDVMRKYPDSLFRPVVLNISLACLAFLICSTLSISVGLIYLMAVPLGTAFILPGIYVWLQRYKKDLGGPWDCAVPRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3L6E0</accession>
    <name>E3L6E0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178358">
        <property type="protein sequence ID" value="EFP92115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336534.1">
        <property type="nucleotide sequence ID" value="XM_003336486.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L6E0"/>
    <dbReference type="GeneID" id="10532075"/>
    <dbReference type="KEGG" id="pgr:PGTG_17420"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17420"/>
    <dbReference type="HOGENOM" id="CLU_1548376_0_0_1"/>
    <dbReference type="InParanoid" id="E3L6E0"/>
    <dbReference type="OMA" id="WITNIID"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="173" mass="18542" checksum="675DFEE6B4102D0D" modified="2011-01-11" version="1">MNTEPPDVEPTGQAGQSTSLGGGEQGGQSGTPDGGDGEGEHSWISTIIDKIIPNLPGAGNRNTSAGNTAPMDSQPSGTLNTDGMTDQSRLDPDIVIEHARGARASVEERGNQIREERRARGIAKKATLNEHCNKYSRGIQHFIKFFLGGPTIPQDYPQSPTSEELEALYWVDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3L7K1</accession>
    <name>E3L7K1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18524</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178366">
        <property type="protein sequence ID" value="EFP92526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336945.1">
        <property type="nucleotide sequence ID" value="XM_003336897.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L7K1"/>
    <dbReference type="STRING" id="418459.E3L7K1"/>
    <dbReference type="GeneID" id="10538523"/>
    <dbReference type="KEGG" id="pgr:PGTG_18524"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18524"/>
    <dbReference type="HOGENOM" id="CLU_029875_0_0_1"/>
    <dbReference type="InParanoid" id="E3L7K1"/>
    <dbReference type="OrthoDB" id="2507141at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="227"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="460"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="488"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="509"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="528"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="547"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="592"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="606"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="655" mass="72928" checksum="3BF725C85FAEB74F" modified="2011-01-11" version="1">MNHSTISEGQATTTTLAISQVQVPLTSTTSQSQATTSTISQGPAPDPNSTISQHPTHNNHFAANNEDEDGLRGTMEDDHNIDEASFRKNQMKLSDSQMEPFATMDLAALTAASKSYSTRRRMTDDMKEEVDEMYYEFQCNIAKLSIQNRVGAHLYFDHVGQSRRVRAGTSWNNFQKYDPEAQKMFDEFGLNVGRSKVSAIWARKTWPEKLRYRDVDYLKTLREVTTNSGESNVPHPGTVLHAPTGSSPTRLNGRVQLSKVSLEKTSSLVANWVTKIQSDLDSFAFFHQVEGFYVIASRHPKSTIFHKGGSSVGNKFLRMLAQNKETDPAADFHTWTAAQAIQLSNGCGTTLHLQSRIKTVDDVAEKFRAGKHADNIKAIRDKLRHLIYVASGHKLNSPWPGEDTDYRLQELKFSFSIDDNQWNIEPNDLKKPLVKLNCGTAMGILACLGLNKIHLTYHPDWEDISPRPRKKQSNTNKRKNKRNRKDDENEDEDEDEDEDNEDNNNNKNPSESNSGNQPQHKRLRINAATDNGFQAGNQPQRKRTRTDAATNSSTADNLSESGNQPQNHPNRNDGATNGSTTLNKKKRFRPIKTASKTLSATTADTNQLESNKGNPADNNRADVSGEGNPPGEGATNANETPDNDPTLDPLLAALV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8P9</accession>
    <name>E3L8P9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_19053</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178376">
        <property type="protein sequence ID" value="EFP92935.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337354.2">
        <property type="nucleotide sequence ID" value="XM_003337306.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L8P9"/>
    <dbReference type="GeneID" id="10542827"/>
    <dbReference type="KEGG" id="pgr:PGTG_19053"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19053"/>
    <dbReference type="HOGENOM" id="CLU_906530_0_0_1"/>
    <dbReference type="InParanoid" id="E3L8P9"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP92935.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="307" mass="33990" checksum="ED7C8FC9AA14FB08" modified="2012-04-18" version="2">MSATCHSPEFKLQLNLDDGRHGILSSNSNHTTACRTQFCCLLNKSNLTFPVLITPRIRTYDEGSFSAPSPQPTATGQYCWSSTAVIIKEADLKYNGENFEDFLHRFKLAAEIYGAGGFDKALQHDGTGKDGYNKPPLLADVIEVAKDEIRARSINVFAASGFAEANHMMQRSLDQKKGDARKREKMNTHKGMIDPLGGSMNLNPVGTVTEKDIPRRTENPAVFSTPLQLPPSTNKHEYISSLHKIDWDPPRLGSGNFEKVKLKANTATTQAEALRGQCKTPVKKDNLEMDVDQLDELEELVQDIPAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>E3L9R6</accession>
    <name>E3L9R6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_19059</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178388">
        <property type="protein sequence ID" value="EFP93291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337710.1">
        <property type="nucleotide sequence ID" value="XM_003337662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L9R6"/>
    <dbReference type="GeneID" id="10543523"/>
    <dbReference type="KEGG" id="pgr:PGTG_19059"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19059"/>
    <dbReference type="HOGENOM" id="CLU_1525912_0_0_1"/>
    <dbReference type="InParanoid" id="E3L9R6"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP93291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="176" mass="19060" checksum="63E4D9B2FE453DEE" modified="2011-01-11" version="1">MPTWQPLGCQVGEAMERDSQPTQQPITRDHAHLAAKWASEINAGGGLTSVLNWIGGFNNRRRSISGAVERDSQPNHQPRKREHAHLAAKWASEIKAGGGSPLFELDRRGQNRSAIVFWNCGKGQSAHPSAKNKGPCPLGSQLAAKWASAIKAGGGSPLFKSDRRGQKRSAIFFRGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3LAH8</accession>
    <name>E3LAH8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19549</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178397">
        <property type="protein sequence ID" value="EFP93553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337972.1">
        <property type="nucleotide sequence ID" value="XM_003337924.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LAH8"/>
    <dbReference type="GeneID" id="10543829"/>
    <dbReference type="KEGG" id="pgr:PGTG_19549"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19549"/>
    <dbReference type="HOGENOM" id="CLU_1182630_0_0_1"/>
    <dbReference type="InParanoid" id="E3LAH8"/>
    <dbReference type="OrthoDB" id="10273244at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174522" evidence="1">
        <location>
            <begin position="21"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="265" mass="28974" checksum="A30CE151CE28BD94" modified="2011-01-11" version="1">MRFRTNHYIIHAMFFGLCAAMDDALNQSYLANHEWETIPPMWNDMASSGTPVGSLHHINDVSAASSLSHRQEIHHENVVSGQHYPLDSSGMAVGSRNNVPDFLSEFDSENHGVGGFQMRGRPDHTIHQSISQAKGTPFVHSGINSVSGAALSGIRKRKGRGSETALLSPTTTSIQLESIQGPATDGSSVKTLSRVHFRKKEGKRRGSARQSPLVDLTAISDEEKHGSINNRIKTTASSKFLLNSLANRAHSLFVSSAIRDILQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3LAS4</accession>
    <name>E3LAS4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Large ribosomal subunit protein bL21m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_19598</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL21 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178401">
        <property type="protein sequence ID" value="EFP93649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338068.1">
        <property type="nucleotide sequence ID" value="XM_003338020.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LAS4"/>
    <dbReference type="STRING" id="418459.E3LAS4"/>
    <dbReference type="GeneID" id="10534938"/>
    <dbReference type="KEGG" id="pgr:PGTG_19598"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19598"/>
    <dbReference type="eggNOG" id="ENOG502S8F0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089399_0_0_1"/>
    <dbReference type="InParanoid" id="E3LAS4"/>
    <dbReference type="OMA" id="QPAHYIT"/>
    <dbReference type="OrthoDB" id="5994at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028909">
        <property type="entry name" value="bL21-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036164">
        <property type="entry name" value="bL21-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21349">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21349:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL21M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00829">
        <property type="entry name" value="Ribosomal_L21p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141091">
        <property type="entry name" value="L21p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="249"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00044129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP93649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="272" mass="30123" checksum="A202DBFEC983E4A9" modified="2011-01-11" version="1">MAGLNRLFGTLSSLISTTTECTMVRKSPLLRHSQSPCASSSTSFIRSNSTLVIRPTLQTGLKRANRGTRGLPKIPSSEALGMLKSEDNHYLVTFLVGKKYKLMVDDQVTLPHIKDLKVGDLIKLTRITEVGSRNFTLRALGNGTVGHAKRETLSPAASKTLGWKAKKLGMDPAELSREARTVCFKPEIPHYLDEDLVHASAIVVEHTRGKMSTVIKKKRRKGYRKTIKNKPYYTRIRLCEIKVPTIQADSSQPRIPSMSADPSATHQSTITV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3LC25</accession>
    <name>E3LC25_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Golgi to ER traffic-protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_20063</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="entry name" value="MICROTUBULE-ASSOCIATED PROTEIN"/>
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        <property type="entry name" value="RETICULOCYTE-BINDING PROTEIN HOMOLOG 2A"/>
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            <begin position="1"/>
            <end position="144"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <end position="317"/>
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            <begin position="57"/>
            <end position="105"/>
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            <begin position="804"/>
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    </feature>
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            <begin position="828"/>
            <end position="855"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP94100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="855" mass="93158" checksum="3DEAAD09F85B2640" modified="2011-01-11" version="1">MAPSSPDRPSFSDEEDEESFNLATLGAPTPAQPTNKGPASTQEPQTLRTTQANPAANNTTTTNNNPNNNPNNNPNNNPNNTNNNTNTNNSNSNNSNTTSNNNTTTTKKKKSPKDVVTEGQEVRHGVDMPTGKVAQISSTPGGGKEVRLSALQSLEKKYQDAKATLAALLSEATETLGAPPSLEGNLFAKMDEAFDLRLESWKVKIEEGVTTKSEVVVLDDDVVEVPRSKKRPRASDKASLPTFSKKQKSAGAAGTQAGKSAPPPASSKKGRGKEQESLFMDDEDDRYKDDVDVDGNGNEKTGKGKDGAGAGTEKEKEKEIEDNSVIKKGHFLSALVLCRYSLPVGLKEIYTQSLGALEPKQAAQNAGNTSLEFGTLASPILFRFLNWIPSAIGHIQVNKYELASRMNTQLMGAPRKKPFFELIRSAPGLIQVSYPDPFWNKDLIDFDKIQTASKENPKDPVGGWTALLNLICRAEGDNTLGYTTHVSRKALISAAFNRVSQLTRDSVAAISTNIKPPKEDPKAPKTDLDGLRSAGKFLHHRIEALGGSTPTSGKKGQVKASEEGISFLQKRIFEMLLGIALCVECNELFKKEKEYERLPGLTQEEIKAKKSELQSIAVFRTGPGTISTADWSIARKEAYAALSIFLTFGVAGWFSSFTNHRKYNLADCYGLITLADQRIEQLKKIPADGIETTHPLVSGGWKYLNGFLLGVLNASKIPIADSAQWRLMSKKWAETLTPAQIGELVLADIFAEVAQPGPSVSLTGKYAVHPVIDAGTEKNKDGVLQILKKTMPFLLKGSHGGLGDGEEDEDEDEEEDEEEDEEEEEEGREERAEHERRAQEAVKQFIHDEADCSDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3NXG0</accession>
    <name>E3NXG0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20188</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS989935">
        <property type="protein sequence ID" value="EFP94259.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338678.2">
        <property type="nucleotide sequence ID" value="XM_003338630.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NXG0"/>
    <dbReference type="GeneID" id="10527348"/>
    <dbReference type="KEGG" id="pgr:PGTG_20188"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20188"/>
    <dbReference type="HOGENOM" id="CLU_030899_0_0_1"/>
    <dbReference type="InParanoid" id="E3NXG0"/>
    <dbReference type="OrthoDB" id="2510764at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="397"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="491"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="128"/>
        </location>
    </feature>
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        <location>
            <begin position="196"/>
            <end position="210"/>
        </location>
    </feature>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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            <begin position="447"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP94259.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="512" mass="57234" checksum="01449E1ACF8DE156" modified="2012-04-18" version="2" fragment="single">STPSNVSTTKDSKAKQASTAEQTSAAQSITAPKRSSTKIPKKKAKKPISPEIVPSEWDTSSDEATKSDKPKPPSSIAPGLKKSSTTSDPFKSNKPVDKLVDSDLLKNINFKKSIPKGTSTATTVSIVQPTEPINEAKNQQPDLFQHPVDSSAAQTTIHPAPVSSVLPSLPKISSDATVQKKLENYFVPQGRTTRSISSSSSVLPSGSFTTEPYEPADSSDLDIITGATAQLWSKPKISKGPLEEDILQKYRPSFHPLLQTVKLQREYYQKAIETKDEDLKIVALRAASSAQSDLLRAMNQEEFLDLFNRWDPISEYQDYLTGQSGRNYWRKEGVAVTPSPSPEVEMRPPESVQRNQTTDSSNQRQAQMDFHYSAENPPPSANIQPYEQYRNAQNSPETIQGHQHYPTEAHNRPHSANNRSRNRNRNSNNHRNQEYRATNFRPRNPNGPRQRTNSQTARENRCIAHQRSIHQEMIRLSRTTQAQYQALEAMREQSAGYGSRRQPQEGGANQNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>E3NXX6</accession>
    <name>E3NXX6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">hAT-like transposase RNase-H fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20381</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS989989">
        <property type="protein sequence ID" value="EFP94425.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338844.2">
        <property type="nucleotide sequence ID" value="XM_003338796.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NXX6"/>
    <dbReference type="GeneID" id="10527635"/>
    <dbReference type="KEGG" id="pgr:PGTG_20381"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20381"/>
    <dbReference type="eggNOG" id="ENOG502SPG0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013565_2_0_1"/>
    <dbReference type="InParanoid" id="E3NXX6"/>
    <dbReference type="OrthoDB" id="3359487at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052717">
        <property type="entry name" value="Vacuolar_transposase_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46169">
        <property type="entry name" value="DNA REPLICATION-RELATED ELEMENT FACTOR, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46169:SF15">
        <property type="entry name" value="INNER CENTROMERE PROTEIN A-LIKE ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP94425.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="310" mass="35432" checksum="282E074C6FD0A985" modified="2012-04-18" version="2">MVRELKKLKWPRFHGDAQWIRCFAHILNLIVQGILWPFGSHKKDAKDRGPMGAGDDSDGSISEQEDPEDQIRVLPRGRQDNSATSAGDKLSDEESVYGADVDDDESLDEANIDNASEEEEEDCYTSISCKQTLAKFRAIAKKLRFSPASKAEFYKICQQKRCEKPHNIERDVRTRWNSTSIQINSIIRCQAAILEWQRHKTYGIDRVHYLDQSDLDLAHDLAKVLNLFHNITLQISKAGSARLSNKVIFIDQITDHLSTIISDSNYPPALRNACQVGLKITNKYYSLTDTSPLYRIAIFLHPSFKDEYQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>H6QNT7</accession>
    <name>H6QNT7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7872 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20645</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EHS62525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890842.1">
        <property type="nucleotide sequence ID" value="XM_003890793.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QNT7"/>
    <dbReference type="GeneID" id="13542492"/>
    <dbReference type="KEGG" id="pgr:PGTG_20645"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20645"/>
    <dbReference type="HOGENOM" id="CLU_030195_2_0_1"/>
    <dbReference type="InParanoid" id="H6QNT7"/>
    <dbReference type="OrthoDB" id="2501761at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057194">
        <property type="entry name" value="DUF7872"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33339">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33339:SF1">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25278">
        <property type="entry name" value="DUF7872"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7872" evidence="1">
        <location>
            <begin position="181"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="401" mass="43736" checksum="0F7F0E571B75A877" modified="2012-04-18" version="1">MTRSCDPLPLVPETWDELGLDDYLKNYPNGDKIHLADYVGSKNASNFVCGIGSICDAGQMCYPVTDQDWYILFALQQWNSISNMAFAAIGYAINIVQATVNSLISTMFPAVDISGATHGKQSLAISATMMEVSGTLMMDVMMLMGAETGPVGWVLNVLNFAMAGGMAIWAFKRKVPKGPIVEGFGVWTDVVFQLAALEENAQNVISDAVKKVINSPISSDDGIYGVLKGGAFLNPTKMLVIPEVADHLRKVTMAMSMNLVLRSMNSYITIGADVCTDKGENGAWPQKDRLSYCPEHGGPMYNIIRAVGDKSVNAIPNANVVNEIFEFSTKMIIEVSVNCQQKHNEFGHNPYTKGNFPKSETDECVFNVPVCDCRSGDIRSSIKDHKQHVTEACRKHGGLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>H6QNU9</accession>
    <name>H6QNU9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_20614</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EHS62491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890808.1">
        <property type="nucleotide sequence ID" value="XM_003890759.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QNU9"/>
    <dbReference type="GeneID" id="13542553"/>
    <dbReference type="KEGG" id="pgr:PGTG_20614"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20614"/>
    <dbReference type="HOGENOM" id="CLU_1993723_0_0_1"/>
    <dbReference type="InParanoid" id="H6QNU9"/>
    <dbReference type="OrthoDB" id="10340001at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS62491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="125" mass="14450" checksum="5A6C2DEC22E22B6F" modified="2012-04-18" version="1">MDLLRWLFVTAAIIATNRRPSVTHLTNTPTFITIKAPRDISQAPTQNTAFKALLGQIKIHPRYQDERTQTLIKLTIRVIILAGLYEWLRTFKTPYNNAPFTGRDYVELWRPATMGCFFRGGMYNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>H6QNY9</accession>
    <name>H6QNY9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Plastocyanin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_20592</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EHS62469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890786.1">
        <property type="nucleotide sequence ID" value="XM_003890737.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QNY9"/>
    <dbReference type="STRING" id="418459.H6QNY9"/>
    <dbReference type="GeneID" id="13542546"/>
    <dbReference type="KEGG" id="pgr:PGTG_20592"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20592"/>
    <dbReference type="eggNOG" id="KOG1263">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_727886_0_0_1"/>
    <dbReference type="InParanoid" id="H6QNY9"/>
    <dbReference type="OrthoDB" id="2503026at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13883">
        <property type="entry name" value="CuRO_2_Diphenol_Ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF414">
        <property type="entry name" value="ADR239WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plastocyanin-like" evidence="3">
        <location>
            <begin position="27"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="291"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS62469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="380" mass="42667" checksum="62F170D7DB6DD85E" modified="2012-04-18" version="1">MADGINGPMIIHSPRDPLKRSVDFDHDVVLMLTDWYHNTSSDIVHELLSEAGHIGTPAAPGANSALINGVGEWNCSLATTSQQCKQVSSPPEFTVTAGQRIRFRLINSGVHAMFFYSVDEHMLNITEADSTPIYGPSDFRHIWLHNGQRYSVIVQTKKEDAGRSFHMRATMDSDCWAWIPNDIQDTAFGILRVVNEDTKPEKISKARPKTRGWPQEFPEECLDIDPRMMVPIVKRSVPTSVVGSGRFEAGFGFQFINNPQEHLTRNDAVKIAKALNLDWTRDRNLREKKKFLSESHRTSSRWGGNDVDSSEDTHDSQQESHSLNADSRKRQLNIRRQSPPPFGGRKNSTGVTRLAGPPPTPAGTIGKYFMNNISWAKIPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPM2</accession>
    <name>H6QPM2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20821</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EHS64124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890533.1">
        <property type="nucleotide sequence ID" value="XM_003890484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPM2"/>
    <dbReference type="GeneID" id="13542931"/>
    <dbReference type="KEGG" id="pgr:PGTG_20821"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20821"/>
    <dbReference type="HOGENOM" id="CLU_2334665_0_0_1"/>
    <dbReference type="InParanoid" id="H6QPM2"/>
    <dbReference type="OrthoDB" id="10622086at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="98" mass="10402" checksum="DC4B77F5E214DD9C" modified="2012-04-18" version="1">MAMLVSEDSPEDESELLSKDSAPPSAQHPPKLASEESLSSDSEDSSTSCSDATSPKNPPPPQPSSIPPNRDGRAGLYRKGLVAGRRKTLPDQASRPFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQ64</accession>
    <name>H6QQ64_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21026</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EHS64676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890382.1">
        <property type="nucleotide sequence ID" value="XM_003890333.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQ64"/>
    <dbReference type="STRING" id="418459.H6QQ64"/>
    <dbReference type="GeneID" id="13542743"/>
    <dbReference type="KEGG" id="pgr:PGTG_21026"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21026"/>
    <dbReference type="HOGENOM" id="CLU_095782_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQ64"/>
    <dbReference type="OrthoDB" id="2505730at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040521">
        <property type="entry name" value="KDZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18758">
        <property type="entry name" value="KDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="202" mass="22865" checksum="36B59947B0FF4362" modified="2012-04-18" version="1">MFSRIRLIQQKILIEGLQLSQNDQWANQCPRCFGPNQHEVKSHPKEPDIIIALDGNFQQRHYAYASKDNPPESHYPRSFVKPSKITNVADTLTATDASAVGIDPPCADVHKAANDARGETTWEKCDDNGLFASACRHDIPLMFANIYKTGEKLYYPIALVRNLLADFPQHKVGILYDIGCHLERHVRRMESVLRGSGLSYHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQ69</accession>
    <name>H6QQ69_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21031</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EHS64681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890387.1">
        <property type="nucleotide sequence ID" value="XM_003890338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQ69"/>
    <dbReference type="GeneID" id="13542742"/>
    <dbReference type="KEGG" id="pgr:PGTG_21031"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21031"/>
    <dbReference type="HOGENOM" id="CLU_2147102_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQ69"/>
    <dbReference type="OrthoDB" id="10362007at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="112" mass="12752" checksum="58D995C9E0C98A08" modified="2012-04-18" version="1">MDPQNVPILTEAEKGIQICDAIHHLQMTPKKFINGFLTNADPQIAYRRRFWGTSTGSSLTHGIIQAVKAEVASKGRRTGEVLRVSNKEQTFENRLRVRQMTTDRRVSNKEQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQD0</accession>
    <name>H6QQD0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21091</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EHS62542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890255.1">
        <property type="nucleotide sequence ID" value="XM_003890206.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQD0"/>
    <dbReference type="STRING" id="418459.H6QQD0"/>
    <dbReference type="GeneID" id="13542232"/>
    <dbReference type="KEGG" id="pgr:PGTG_21091"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21091"/>
    <dbReference type="HOGENOM" id="CLU_003292_8_2_1"/>
    <dbReference type="InParanoid" id="H6QQD0"/>
    <dbReference type="OrthoDB" id="5149081at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052055">
        <property type="entry name" value="Hepadnavirus_pol/RT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050:SF7">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS62542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="496" mass="57042" checksum="DEF1825E12D39FEF" modified="2012-04-18" version="1">MNLSEWRLALINAKLLPELSHVLTGFEFGFDQGIPVHKLGDLKWYTPDNHSSASDSADKIQKSIKEEVENKRMFGPFSHEEVRQRFPFFRTSPLGSVVNSDGKIRPINNLSYPRNDDKIRSVNSFVNKLNFSTTWDDFKTVASFFRNRTEPLKLALFDWAKAYRQIPTLMSQWPYLMVKDLDGGLYVDTRITFGGVAGCGSFGIPADAWKRIMEYEFDVVKIFRWVDDNLFIKTVDSACVMENITERSVKLGVATSMGKCSEFADEQKFIGFIWNGVNKTVRLPDKKLSERKSQIEVFLVGDTEFTFNQAQVLAGRLNHVAFILPQLRCYIRSVYRWMNQWKKQWATRKIPEDASFGIGVLVGRRWVQLRLKENWRISTPPRGIAWLETVAIRIGILMLQEMRIATRGSNFIVYTDNTTTESVLASRKSKDHHSNEEWKKIQGLLIESEMDLTPLRVISAENAADGLSRGIQRPHVSVDRVWINIPGDLQDFIFHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQJ6</accession>
    <name>H6QQJ6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21203</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EHS62696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890242.1">
        <property type="nucleotide sequence ID" value="XM_003890193.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQJ6"/>
    <dbReference type="STRING" id="418459.H6QQJ6"/>
    <dbReference type="GeneID" id="13541295"/>
    <dbReference type="KEGG" id="pgr:PGTG_21203"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21203"/>
    <dbReference type="HOGENOM" id="CLU_005350_1_3_1"/>
    <dbReference type="InParanoid" id="H6QQJ6"/>
    <dbReference type="OrthoDB" id="2506718at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000026">
        <property type="entry name" value="TBC (Tre-2/Bub2/Cdc16) domain family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050302">
        <property type="entry name" value="Rab_GAP_TBC_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219">
        <property type="entry name" value="RAB GTPASE-ACTIVATING PROTEIN 1-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47219:SF20">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="1">
        <location>
            <begin position="26"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="296" mass="33072" checksum="C51BADD974F2FA0B" modified="2012-04-18" version="1">MGLSNDNNKKALKDEWKEFKALVIQGIPISLRPKIWLECSGASELKEPGYYHDLLNLHDGEEGLCLNQIECDVTRTLPTNVYFGGDGPGVSKLRRVLAAMSWHNPVVGYCQGMNMVAATLLLTIPSEEDAFWILVCIVDKILPSHYYTSHLLTSQADQRVLKVLVSKYLAELADHFDALDVELPAITFGWFLSLFADALPIQTLLRVFDLFLIDGSLLLFRISLALLKINQTTILSYDSPASLYAYMRGPMTLSSHHADLLINVATVDFADIKNSLIVSLRDKFVDQIKVEMGLPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRI7</accession>
    <name>H6QRI7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA mismatch repair proteins mutS family domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_21511</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA mismatch repair MutS family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EHS63295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889868.1">
        <property type="nucleotide sequence ID" value="XM_003889819.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRI7"/>
    <dbReference type="STRING" id="418459.H6QRI7"/>
    <dbReference type="GeneID" id="13540687"/>
    <dbReference type="KEGG" id="pgr:PGTG_21511"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21511"/>
    <dbReference type="eggNOG" id="KOG0221">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_264935_0_0_1"/>
    <dbReference type="InParanoid" id="H6QRI7"/>
    <dbReference type="OrthoDB" id="29596at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019239">
        <property type="term" value="F:deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030983">
        <property type="term" value="F:mismatched DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051026">
        <property type="term" value="P:chiasma assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03281">
        <property type="entry name" value="ABC_MSH5_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002844">
        <property type="entry name" value="DNA mismatch repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1420.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001365">
        <property type="entry name" value="A_deaminase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000432">
        <property type="entry name" value="DNA_mismatch_repair_MutS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007696">
        <property type="entry name" value="DNA_mismatch_repair_MutS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036187">
        <property type="entry name" value="DNA_mismatch_repair_MutS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045076">
        <property type="entry name" value="MutS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361">
        <property type="entry name" value="DNA MISMATCH REPAIR PROTEIN MUTS FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11361:SF20">
        <property type="entry name" value="MUTS PROTEIN HOMOLOG 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00962">
        <property type="entry name" value="A_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05192">
        <property type="entry name" value="MutS_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00488">
        <property type="entry name" value="MutS_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00534">
        <property type="entry name" value="MUTSac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00533">
        <property type="entry name" value="MUTSd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48334">
        <property type="entry name" value="DNA repair protein MutS, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00486">
        <property type="entry name" value="DNA_MISMATCH_REPAIR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA mismatch repair proteins mutS family" evidence="6">
        <location>
            <begin position="678"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="93"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="106"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS63295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1260" mass="140173" checksum="AB6264B57DCC623F" modified="2012-04-18" version="1">MGGAYYDPSNGIFYLMEDAVDTGGEFEVLIMLLEQVMPEVVLLSSKAEESLTSCIQKAECPGGIDKTPTVVKRPSREYSASDGFKKLQRLSVRSQDGVVPSGNHHSSSTSTRSTASQDPGRDIHFSKNSNLTANAFINVEASHYSLGAVAALLSYLQSGIARDQDNDSPQGGLFVSKVEMIKTQKFMHLGNDAIQSLQIFDQEAHANSHFSKTKEGLSLYGILNECITHNGKLLLRNWLLRPSTDPKVIGSRQDAIECFLEPENGVVILLLSLFASLPSVNQLLDLAFSADYVGKMRQDLKLLGNINRTTMIISSGKSRVGEWKSLTNSIHACQALYEDFSKMNLTIETPLFIKMKSRDRLEIDLEPLASNIEELIDWSESKLNGGRVTIRSDVDEELDGYRGQYASMETQLCFIAEKVSTETPFKSLPGFSINYFPQIGYLCAIPFPTPSGSVDPSTLEDWEFQFSTDTEEHYKNGLMRELDDHYGDLATAIDDREIELVHILEEEVSKFASILLSVNDDLAELDCLLSLAKVTFERTWVRPKILNQNTIQIIAGRHPLVEGCVENYIPNDTHLVASAPSKAQRKKRKSTESYTGNPMIVLTGANFSGKSVYLKQVAMIVIMAQLGSFVPAEEAHIGIHDAVFTRVSTTESASRSSSAFMIDLLRVSFMLRNCTARSLLLIDEFGKGTDPTDGQALFCGVIEHLISRGSSCPITLVSTHFHGVFTNGFLSLDLPIDYTHMCIVLKDGGGPEEENSPTYLYKLAPDLVSSSHAMGCAAQAGVPRHIRLKADRVSRLLSRHEILELLDVRLDEDEREELGRMEETVRRFVAIDFEQDGLSSCRDWMAYLRENVLENERTRAVKTIKVDFRSLEFQKAPRLPVSGCRGEVEGFGKGGQTVGGKVYKKLPEEAWLAGMDSKTFCQKLPKIECHAHLSGSIPLEVLQELHKDAKERQPNLGACPSPSDYTDLQSFFTLFGEHIYHILDTPEAITRATSAVLSSFRADGCIYLELRTTPRKFRAGSDSFSEYLDAVIEAIDRNDESKMMVRLILTVNWDFGPDQVKEIVQLATKARDAGRCVVAIDVAGDPQMSIFRTDGFTRELVKAQVNGLKLTIHFAEVVEQRPFLEKQLTELKPDRLGHAVFLTAEVAETIVQQKRPIEICLTSNLKVGSIRSLEEHHFAWAVNNHVPVLISTDDTLVFGTTLSEEYEWALSLLNHDRQKLVSLLKESITCTFCSPEDQVALIQKIDQFSADPSNEVSKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>H6QS34</accession>
    <name>H6QS34_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21608</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EHS63480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889691.1">
        <property type="nucleotide sequence ID" value="XM_003889642.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QS34"/>
    <dbReference type="GeneID" id="13542832"/>
    <dbReference type="KEGG" id="pgr:PGTG_21608"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21608"/>
    <dbReference type="HOGENOM" id="CLU_2470174_0_0_1"/>
    <dbReference type="InParanoid" id="H6QS34"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="88" mass="9229" checksum="99F2C58A7EA5A93D" modified="2012-04-18" version="1">MVHVTSWPVLAREVNEGQPLPVFWAAYGLPSDSRPPPVGTAADFSNLSFAAIAAAGNSSRTSSLTSWGTYAVSPVGVARQNNDPLSRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSG5</accession>
    <name>H6QSG5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21781</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178294">
        <property type="protein sequence ID" value="EHS63702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889524.1">
        <property type="nucleotide sequence ID" value="XM_003889475.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSG5"/>
    <dbReference type="GeneID" id="13542765"/>
    <dbReference type="KEGG" id="pgr:PGTG_21781"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21781"/>
    <dbReference type="eggNOG" id="ENOG502ST2C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003292_5_1_1"/>
    <dbReference type="InParanoid" id="H6QSG5"/>
    <dbReference type="OrthoDB" id="2506287at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052925">
        <property type="entry name" value="Phage_Integrase-like_Recomb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34605:SF3">
        <property type="entry name" value="P CELL-TYPE AGGLUTINATION PROTEIN MAP4-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34605">
        <property type="entry name" value="PHAGE_INTEGRASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="211" mass="23296" checksum="E22E6BCBCEDC669C" modified="2012-04-18" version="1">MQLFTALYQTSDFDNALIDLCIVAFWGLARLAELTYDRKDGPVRCDMSVLTSDVTFTETSNGMGVTAAITLRAAKTAGPGGTQLILLTEQPHILCPVRAIRRRLAEANGERTSLFGFGRPASRHHITRRAAVTRIQAIFATGGGNQLLGHSFRVGGASLRHALGMKPDQICSLGRWTSHCYRLYLRPYSREDMLHTTSLLRALVSNWSKMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>H6QT13</accession>
    <name>H6QT13_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21971</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EHS63967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889329.1">
        <property type="nucleotide sequence ID" value="XM_003889280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QT13"/>
    <dbReference type="GeneID" id="13541894"/>
    <dbReference type="KEGG" id="pgr:PGTG_21971"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21971"/>
    <dbReference type="HOGENOM" id="CLU_1215297_0_0_1"/>
    <dbReference type="InParanoid" id="H6QT13"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="220" mass="25066" checksum="7D1B693A4E8DD9DB" modified="2012-04-18" version="1">MAQPAQKPSSSKLEHQRLCWHYGDLVSYGFRRLQSKYHSRTAELPDLTYIENALAALSLDTVQTQEALANQLQSSLLPLLQGQINTLFQVLDPAGLRNQPRPKLSLVLQTQAELDDSLDQIKYLTATLCPDPATQPHRTDDHGLERFKSCRLYRLKANVELVLPWRMCEIFEAADKLIQKMELSWEVYGGAQHRVHRRPCSGSRLPRRRPERAGAAGRID</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>H6QT96</accession>
    <name>H6QT96_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hydrophobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22097</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178309">
        <property type="protein sequence ID" value="EHS64042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889259.1">
        <property type="nucleotide sequence ID" value="XM_003889210.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QT96"/>
    <dbReference type="GeneID" id="13541651"/>
    <dbReference type="KEGG" id="pgr:PGTG_22097"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22097"/>
    <dbReference type="HOGENOM" id="CLU_2575006_0_0_1"/>
    <dbReference type="InParanoid" id="H6QT96"/>
    <dbReference type="OrthoDB" id="2508065at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003605618" description="Hydrophobin" evidence="1">
        <location>
            <begin position="27"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="8423" checksum="63B5179DFB2BE122" modified="2012-04-18" version="1">MNFNISKISQAFGIILMMGMVSEILAGASCPSARAFMCSGNSGLYSPPKLQTCNGSDSLMCCLVGLPPPGQDCVAPSYNVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTR6</accession>
    <name>H6QTR6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22158</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178320">
        <property type="protein sequence ID" value="EHS64281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889108.1">
        <property type="nucleotide sequence ID" value="XM_003889059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTR6"/>
    <dbReference type="GeneID" id="13541758"/>
    <dbReference type="KEGG" id="pgr:PGTG_22158"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22158"/>
    <dbReference type="HOGENOM" id="CLU_2590914_0_0_1"/>
    <dbReference type="InParanoid" id="H6QTR6"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="80" mass="8759" checksum="E571F5E9E96A4958" modified="2012-04-18" version="1">MSISGDLCRLSRWTSSSDRATTSLEPEVKKTSSNQTSTKIQNPVLPIPGGLRDPTRMRRPVDQGIHESSSGSENLKNAFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>H6QU03</accession>
    <name>H6QU03_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22253</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178326">
        <property type="protein sequence ID" value="EHS64417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889016.1">
        <property type="nucleotide sequence ID" value="XM_003888967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QU03"/>
    <dbReference type="GeneID" id="13540989"/>
    <dbReference type="KEGG" id="pgr:PGTG_22253"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22253"/>
    <dbReference type="HOGENOM" id="CLU_903552_0_0_1"/>
    <dbReference type="InParanoid" id="H6QU03"/>
    <dbReference type="OrthoDB" id="2505190at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="308" mass="35230" checksum="8C2BD976BF5DE388" modified="2012-04-18" version="1">MADTIDRGLDGLTLSVSEEDQLRRQGDLVIKSFRALGDDHLITNVRPDYPDNRPNHQVDPLDHQTEYHPDPPDSKSSLDRFHWPNRFQLSQQITSLSESLDPSLLRSQPGPTLELILEILSGIRESLDQIKAAFHVRKIGLEKRIQDLALRNQQLFSNSEDLILHLKLSSDEPKFPDEPHWIRARIIHCTADSSEATQRLIEWIEGSELDIVQHGWMSYMRSIDSALVVELERFKLSDTQRELAEHILPILKLSRLLLKKLSKRGLSGEEGIGSYTELSSKQRTSLLEFGSKLHMITMSAKNLFDFIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>H6QVE4</accession>
    <name>H6QVE4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22719</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178405">
        <property type="protein sequence ID" value="EHS62898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888520.1">
        <property type="nucleotide sequence ID" value="XM_003888471.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QVE4"/>
    <dbReference type="GeneID" id="13540827"/>
    <dbReference type="KEGG" id="pgr:PGTG_22719"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22719"/>
    <dbReference type="HOGENOM" id="CLU_2122258_0_0_1"/>
    <dbReference type="InParanoid" id="H6QVE4"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS62898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="114" mass="12323" checksum="6CF940290D5EC803" modified="2012-04-18" version="1">MPSTLVDQMESPLPAPGGIVLFNHSQFLFLPFSFSYMTSSTSSPRGSSQSCLVGSEDDAVACDAEPRELDPDEPTMALADPLMPPGSCHSPRFLMFPGTALQLTVTYIFPHWGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>H6QVH6</accession>
    <name>H6QVH6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22711</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178423">
        <property type="protein sequence ID" value="EHS62991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888487.1">
        <property type="nucleotide sequence ID" value="XM_003888438.1"/>
    </dbReference>
    <dbReference type="STRING" id="418459.H6QVH6"/>
    <dbReference type="GeneID" id="13540972"/>
    <dbReference type="KEGG" id="pgr:PGTG_22711"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22711"/>
    <dbReference type="HOGENOM" id="CLU_252092_0_0_1"/>
    <dbReference type="InParanoid" id="H6QVH6"/>
    <dbReference type="OrthoDB" id="2507552at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="612"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="770"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1007"/>
            <end position="1047"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1146"/>
            <end position="1169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="223"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="319"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="400"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="494"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="666"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="684"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="695"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="733"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="846"/>
            <end position="855"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1028"/>
            <end position="1042"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1437" mass="159749" checksum="31DB69C5F2442E8D" modified="2012-04-18" version="1">MKRPAEDRVPLWDDPNQLTIPRLRHTLSVYKISIDQGEPRTKLFQQYVRLARIQSPDSVKFWIGQHSLTGPLPAPDALVVSQIKIFLALHKIDYPAQAKRGHLVHLYQSITPAAPTRRSLIPSSPATSVTVSVVIPPHPEHKKRKANLRQKSKSSPPKPLPKSNALWLVPSHIGKPGIEKLPSHGRSKRIKSAARSETFFAPYSRREHAEDEQPSSAKPRKVSSTSRRSSTTGAADPRSLSHMSESSQHHTLRRSPRLRAAHEDSQMLADLPSHELSLSSVEPTQSSRNEAHDNESRQYGSDDDSDMSNRRGDVVEASTRASSPTPSVSLADSPIHPVPSRESSQDRHSPTEEEASESPFEAPLTPQQPTQDGPESPFEAPGSLISPFGRSSVATTADGSQSIQSSQQSTQEEGPESPFEAASTSRFFEEGAQPPTADLSPRASEEVESSRVQRSSSPVGLGNEPEDSDFRSSEWSSRLADQESEVSTRPLVQAGDSEASSTSPSANHSSESEDHDSEDHDSESSQQSSRPEVSRRRVTDEMTASSRPQPLSGASKKRKSEDPSEWPDASILTRVQLEEYLAAHDVPYDRSTRLARIIGSYNLLRSSLPHLGDLQPFKRPRASQSSPPPSSTGIKRRRTRARVEASSDDDQVGFVYSRPERPRNASIPPQSPSDSGSSDYQPSVAPTETSVGTPSRASNSTRSTIRTTRSFPTENPRQKRAKKGNNRPQTSKKAGKRRARASRPKRTRWRVSTHHEADLASDTVNFVPVLPETSKKRKNRSSTPARAKRACPGPDDLPSSEFATHRQASASRRRQPETRHNTYTPHPSFRDDGQVDLEDVTPGHTEQSEQSDRLGRPSKRTPPAESSSRSVHTKRTRPNPPDFDWSDLELQQSRPPLRPSTSRREGRVRSDSYAPASSFHDDPLASSFHDGGPTQPHLDHPQETHPLATPRSKRRRPDDSSPSPQRAKRRAPSAACLIPSSAHVRRRRAREQRVRLNSLTIPSIHDDFIVPNPSESGVERWRNSVPPGLSPPSRSPSPPPSANPNLRGCALGGCTGMLSELTQAAGRLTDSIVHATNQASLMQSTSLMPITGQPQIPASRPSGPRDLSLLARVRQHIRTLFGKTMELNIFPPPATAAEKALWRRDPENDYPEDEPMSASSASSHDDAVDPCFPYANGPGHRQASASTLKIMWRSMQRCRVVSFRPDFVRAVRDTDNLFLWDLAHSIFIKLVRAQEYPEIDLETCSSAKIYDAILNHAKQLKRTYTQAGWDPRRQDAQAQKKRRQMCTTRLRDARLKFCTTQSFLIPLIPVIDECTSDTESDAGIEGSDMDTGENERIAFVTKLPWRHPRIEEAVQLIDQLIERKRKSGSRDFGRSSSYTRFRYGNPRISDWPCPAQLPAHAYCEDWLKNQRIPELTKLHIQTGPSLKGLVKELKKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>E3JPZ9</accession>
    <name>E3JPZ9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 11">Triosephosphate isomerase</fullName>
            <ecNumber evidence="6 11">5.3.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_00038</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>D-glyceraldehyde 3-phosphate = dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:18585"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="5.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the triosephosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.1" evidence="6 11"/>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307088.1">
        <property type="nucleotide sequence ID" value="XM_003307040.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JPZ9"/>
    <dbReference type="FunCoup" id="E3JPZ9">
        <property type="interactions" value="541"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JPZ9"/>
    <dbReference type="GeneID" id="10527514"/>
    <dbReference type="KEGG" id="pgr:PGTG_00038"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00038"/>
    <dbReference type="eggNOG" id="KOG1643">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024251_2_0_1"/>
    <dbReference type="InParanoid" id="E3JPZ9"/>
    <dbReference type="OMA" id="NWKMHMT"/>
    <dbReference type="OrthoDB" id="6715177at2759"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00189"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004807">
        <property type="term" value="F:triose-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046166">
        <property type="term" value="P:glyceraldehyde-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00311">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000025">
        <property type="entry name" value="Triosephosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00147_B">
        <property type="entry name" value="TIM_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035990">
        <property type="entry name" value="TIM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022896">
        <property type="entry name" value="TrioseP_Isoase_bac/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000652">
        <property type="entry name" value="Triosephosphate_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020861">
        <property type="entry name" value="Triosephosphate_isomerase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00419">
        <property type="entry name" value="tim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21139:SF41">
        <property type="entry name" value="TRIOSEPHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00121">
        <property type="entry name" value="TIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51351">
        <property type="entry name" value="Triosephosphate isomerase (TIM)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00171">
        <property type="entry name" value="TIM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51440">
        <property type="entry name" value="TIM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="9 11" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 11 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00019397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP74082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="255" mass="27703" checksum="C6E7D16896D747CD" modified="2011-01-11" version="1">MAPRKFFVGGNFKMNGTLESLQQLVKSFNEADLDPNTEVVVAPPALYLMPVRDTAQKNIQVGAQNGYLEKSGAFTGEISFTQLIDAKIPWVILGHSERRSLFHEDSEFVGAKTGAALAANLSVIACIGESLSEREADKTLAVVESQLEGIKQGLAKHGQSWDRVVVAYEPVWAIGTGKVATPQQAQEVHKAIREWFKKNVDAKTSESIRIIYGGSVNGKNCEELAREVDIDGFLVGGASLKPEFVQIINSGKSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRD4</accession>
    <name>E3JRD4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_00554</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307604.1">
        <property type="nucleotide sequence ID" value="XM_003307556.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JRD4"/>
    <dbReference type="STRING" id="418459.E3JRD4"/>
    <dbReference type="GeneID" id="10527848"/>
    <dbReference type="KEGG" id="pgr:PGTG_00554"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00554"/>
    <dbReference type="eggNOG" id="ENOG502RDTI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027551_4_2_1"/>
    <dbReference type="InParanoid" id="E3JRD4"/>
    <dbReference type="OMA" id="AYDELAC"/>
    <dbReference type="OrthoDB" id="424610at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050955">
        <property type="entry name" value="Plant_Biomass_Hydrol_Est"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43037:SF5">
        <property type="entry name" value="FERULOYL ESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43037">
        <property type="entry name" value="UNNAMED PRODUCT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP74598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="341" mass="36814" checksum="0003B4AE3D672C4A" modified="2011-01-11" version="1">MSTPASGAPGKLFDIELTVQSSVIPSVFESGPCEKFKPSADGHSQRSVLFFVPESVQGKQNVPLLIAFHGSTENGSIFRSRTTAMAYDKLAAEMGFIVAYPSGYKGNWNDSRKAAAYPAKMENIDDVGFTTSIIQYSAKNWSTCPQRTLIAGYSNGGHMCYRLALELGSRYIAGVAVHCANLPTEDNSDCATLLNDAVPICIVNGTADPVNPWGGGEVTLSHAPVPGGSVGSRGSHQSAVETADYFAARFLDLGVELELEEIEDMDEVQDVRQFRTPRTDKVYVKLLAMIGEGHYVPVASGEKKALVIGPRRGAVHAPREVLQFFAENTMCFEASYPESDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRN0</accession>
    <name>E3JRN0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Alpha-mannosidase</fullName>
            <ecNumber evidence="3">3.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_00725</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Degrades free oligosaccharides in the vacuole.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides.</text>
            <dbReference type="EC" id="3.2.1.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 38 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.24" evidence="3"/>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74769.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307775.2">
        <property type="nucleotide sequence ID" value="XM_003307727.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JRN0"/>
    <dbReference type="FunCoup" id="E3JRN0">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JRN0"/>
    <dbReference type="GeneID" id="10546625"/>
    <dbReference type="KEGG" id="pgr:PGTG_00725"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00725"/>
    <dbReference type="eggNOG" id="KOG4342">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003442_0_1_1"/>
    <dbReference type="InParanoid" id="E3JRN0"/>
    <dbReference type="OrthoDB" id="10261055at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004559">
        <property type="term" value="F:alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006013">
        <property type="term" value="P:mannose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009313">
        <property type="term" value="P:oligosaccharide catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd10812">
        <property type="entry name" value="GH38N_AMII_ScAms1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.50:FF:000004">
        <property type="entry name" value="alpha-mannosidase 2C1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.110.10:FF:000002">
        <property type="entry name" value="alpha-mannosidase 2C1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.98.30:FF:000001">
        <property type="entry name" value="alpha-mannosidase 2C1 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.10">
        <property type="entry name" value="Glycoside hydrolase 38, N terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.50">
        <property type="entry name" value="Glycoside hydrolase family 38, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.98.30">
        <property type="entry name" value="Golgi alpha-mannosidase II, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054723">
        <property type="entry name" value="Ams1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011013">
        <property type="entry name" value="Gal_mutarotase_sf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041147">
        <property type="entry name" value="GH38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011682">
        <property type="entry name" value="Glyco_hydro_38_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015341">
        <property type="entry name" value="Glyco_hydro_38_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037094">
        <property type="entry name" value="Glyco_hydro_38_cen_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000602">
        <property type="entry name" value="Glyco_hydro_38_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027291">
        <property type="entry name" value="Glyco_hydro_38_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028995">
        <property type="entry name" value="Glyco_hydro_57/38_cen_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46017">
        <property type="entry name" value="ALPHA-MANNOSIDASE 2C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46017:SF1">
        <property type="entry name" value="ALPHA-MANNOSIDASE 2C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09261">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22907">
        <property type="entry name" value="Ams1-like_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17677">
        <property type="entry name" value="Glyco_hydro38C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07748">
        <property type="entry name" value="Glyco_hydro_38C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01074">
        <property type="entry name" value="Glyco_hydro_38N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00872">
        <property type="entry name" value="Alpha-mann_mid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88688">
        <property type="entry name" value="Families 57/38 glycoside transferase middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74650">
        <property type="entry name" value="Galactose mutarotase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 38 central" evidence="9">
        <location>
            <begin position="560"/>
            <end position="641"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00054985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00071615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP74769.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1130" mass="127786" checksum="45823AF53FCC2EBC" modified="2012-04-18" version="2">MPSTRDEDYPLMENMPRANRFRNVTLGHLKNFIGGDYGSQNLSSVLYEARDGSESAVKLEVWSAPDLSKPSFAEAVRQKYKKISKGFKFGPSWSNHWVKATLTVPKQYQDRERVLFEFDPGCEALIFTSEGLPLQGITGGTDSAKRIDFIIPKQSRAFPFQIYIEVSANALFGVPDEGQLEVHDDVFFELAMADIAIPRMDAWHLMWDFQVISDLTKSVPVTSPICLKAQTVAMEIMNTFKPDNLETISACRKIAEKILGAEWTQKPIYDPKSDWDLEGDSEGVPVFAIGHCHIDTAWEWPFSVTQQKVARSWATQVDLIQRYPEYRFVASTAQQYKWLEELYPQLFSKVRREVENGKFFVTGGSWVENDTNMPSGEALCRQFLYGQRYFKSRFGKYTDIFWLPDSFGYASQIPQICRQSGIPYFFTQKMSWSEFNKFPHSTFNWIGIDGTQVLVHMTPVDTYNAQANVDEVLKAITNHKDLEWSDKTLLVYGNGDGGGGPLAPMIEKLRRIRSASNNSSNSGVPKVTTGPSVSDFYKMLLNKTRNGKDLPTWRGELYLEYHRGTYTSHGSIKRHNRKSEILMREIEYFATLASLFREDTGYEYPKDELDHLWEIVLLCQFHDVLPGSAIAKVYEDAEKLYAEVAQKGTALLEAARQSALYGSQAVDVVTASSNRGGNVVGLNTLSFPRQEILRVPLEIYDGRSAFSASKTDEDSVFVIAKDATGNGLIELESLSHLQEHIQLATASVGRDVKTGEVCYFLANKALTVQISSQGRIVSIVDTELGRELILSGQSSGFVMYQDQPLTYDAWNADIFHLDTKEIIDAINVKVVEPKGLRASIVVECATISLDAVPGSLKKDALSLVRFETTIDWHEQHKFLKFEVPLDINSDQATYEIQYGVLQRPTHKNTTWDAAKFEVCGHRFADLSEFGYGVALLNDCKYGYACEGSYLRLSLLRASTSPDPRQDQGMHKMRFGILPHRGHFLESDVPQVAMAFNNPIHLRYAPRNISSRNLLAPVTEIFKLDGLGARNVILDTVKRGEDDTFGFHSRKPVRTVIVRLYEAFGGRANVHLVTLVKAQEVFIVDVLERELEKVELKNHQQSITDDQEPYKQVVELNFKPFQFISLKFILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>E3JSR6</accession>
    <name>E3JSR6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_01684</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319510.1">
        <property type="nucleotide sequence ID" value="XM_003319462.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JSR6"/>
    <dbReference type="GeneID" id="10547453"/>
    <dbReference type="KEGG" id="pgr:PGTG_01684"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01684"/>
    <dbReference type="HOGENOM" id="CLU_3051430_0_0_1"/>
    <dbReference type="InParanoid" id="E3JSR6"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP75091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="54" mass="5782" checksum="C48AF58C1214EB89" modified="2011-01-11" version="1">MARLRWYNKTNVSQLPSSNASKLGGGRRGLADYQFLEAVGRTGTAPSLNSIPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3JT14</accession>
    <name>E3JT14_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_01834</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319660.1">
        <property type="nucleotide sequence ID" value="XM_003319612.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JT14"/>
    <dbReference type="GeneID" id="10542615"/>
    <dbReference type="KEGG" id="pgr:PGTG_01834"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01834"/>
    <dbReference type="eggNOG" id="KOG4585">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018552_2_0_1"/>
    <dbReference type="InParanoid" id="E3JT14"/>
    <dbReference type="OMA" id="ANKAYIR"/>
    <dbReference type="OrthoDB" id="2502344at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF85">
        <property type="entry name" value="GH03217P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="8">
        <location>
            <begin position="206"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP75241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="410" mass="47829" checksum="D5451047A70EB71C" modified="2011-01-11" version="1">MRGYSERQCLIRDLFLIIVWLEHEELEDMVDSWFRHIRIPTESEIIFPRSKIQQRMYDLLLSDWAIFDEALRLVLSNRYLVPRPTPKLRDEFDLERLFNMPDIEFRQASRTSKAGFVGLLNIICTNPVFHRGGIRPQLPIAHQLALTLERLGSNGNGASVGRFSRNLSVGRGTVVKVSRRVIEALISLGRRHVVWPDAARRAEISERPGYDGEVFFDRKRRYSINAQIICDCDKYITSFITGWPGSCGDSRVYKRMQLHLNPSNYFDEGQYLLADSAYELSHTVIPVYKVPAANIMINSQFNYCLAKARVRNEHTIGVLKSRWSSLREMRLHLYRRQHMRAYVAWLYSCIILHNLLAGLGDQWAELEDDDSLTYDDDTDEEDIEESAEDFRDRLTINCVCYNMYHRGILH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>E3JU31</accession>
    <name>E3JU31_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">HSF-type DNA-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_00887</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Homotrimer. Homotrimerization increases the affinity of HSF1 to DNA. Interacts with transcriptional coregulator SSA1 on chromatin.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the HSF family.</text>
    </comment>
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        <property type="protein sequence ID" value="EFP75556.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319975.2">
        <property type="nucleotide sequence ID" value="XM_003319927.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JU31"/>
    <dbReference type="STRING" id="418459.E3JU31"/>
    <dbReference type="GeneID" id="10543272"/>
    <dbReference type="KEGG" id="pgr:PGTG_00887"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00887"/>
    <dbReference type="eggNOG" id="KOG0627">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_397479_0_0_1"/>
    <dbReference type="InParanoid" id="E3JU31"/>
    <dbReference type="OrthoDB" id="60033at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000027">
        <property type="entry name" value="Heat shock transcription factor 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000232">
        <property type="entry name" value="HSF_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF427">
        <property type="entry name" value="HEAT SHOCK FACTOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00447">
        <property type="entry name" value="HSF_DNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00056">
        <property type="entry name" value="HSFDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00415">
        <property type="entry name" value="HSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HSF-type DNA-binding" evidence="10">
        <location>
            <begin position="23"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="163"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="239"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="403"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="512"/>
            <end position="629"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="667"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="243"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="259"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="282"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="408"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="434"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="518"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="528"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="578"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="605"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="619"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006403"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00062171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP75556.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="693" mass="77243" checksum="F0E66AF3E2BE9E75" modified="2012-04-18" version="2">MGSIVADDKMTANQSLLQKTGKTQAAFVPKLYIMVQTMKQPTPEEPNPEQPLISWSKAGDVFCVFDPIEFSRVILPLHFKHNNWQSFVRQLNMYGFHKVNDLLSSSHSQTDTVQAWEFRHPFFKQNRPDLLPMIKRKSTKHHGASKANSSLANVQATSQAGEIHFQRQMPLSNGIPGRTARTFESCMPHNQPSPVLPSAHHPLHGKPPPPPSLPTGKANIPPRAPALYPILTHQFQYTHSTHSQLKSSSPLLKHSSTKVSPSTRANPQNNCFIPCSPHSVRLPSNTSRPITSQSQPSRRGNVEEEEVTERSFAWSSVREACGGSSPFTRIEGELRRLNDTLTHTDPKGNHIFSVLGLIVELVTLLVDNQESKVAPSQQEKLSRYVQTAKEALGKLEAANLDSVRRCSGTPSTDSMSPISTDPLERSTLPPRNGCRTSVDSNLLETHKDSSSSTGWIARPRWSDRMKSSQSLPPLNTLIPTYAPPMYHRTHSAGALPASINRLMNADPEEYPKIAASPEEDRQHREQTQEEGEQEDDEVEQAEEEEEEEAEQEEEDDLEDEDDNPDYDDHDTDDEDSEEGGRGRGEDIYGDSKELVDAEAPSEVDQQSFSDQLSSSAGSTSERTMQDSSATALKIQCLSRNDELDILHRKRSYNGSEEEPCAISILTDANTTNHNRKRIRSEQDMDTPTACPTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUG5</accession>
    <name>E3JUG5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_01021</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75690.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320109.2">
        <property type="nucleotide sequence ID" value="XM_003320061.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JUG5"/>
    <dbReference type="FunCoup" id="E3JUG5">
        <property type="interactions" value="159"/>
    </dbReference>
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    <dbReference type="KEGG" id="pgr:PGTG_01021"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01021"/>
    <dbReference type="eggNOG" id="KOG4391">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029375_2_0_1"/>
    <dbReference type="InParanoid" id="E3JUG5"/>
    <dbReference type="OrthoDB" id="10249433at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277">
        <property type="entry name" value="ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12277:SF81">
        <property type="entry name" value="PROTEIN ABHD13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="domain" description="AB hydrolase-1" evidence="2">
        <location>
            <begin position="107"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP75690.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="321" mass="35844" checksum="6D58F9C4F5DDA8B3" modified="2012-04-18" version="2">MTSIYWSWLMKIARLGAWVLKFNAALVGVIGVLLYSFQGKMIYPSSMPQGSREKVPKPSDFEMKDWQEIELVAPDQVVTKAFVILAARRSEKSLSGKALEDWRRRRPTILMLHANAGNVGHRLPIAKVLVERYECNVVAISYRGYGHSSGTPSEKGILLDSQTAFDYINSHPIFDSSPIFLYGQSLGGAVAIGLAANKVNKGKITGVILENTFANLRKLIPVVMPIIGPLSFLCHQTWYSDQRMTQISDGSDSPAFLFLSGTKDDLIPTSHFRILYDLCPSKQKSWKAFKDGNHNDTCLQKDYFPTMGSWIEEIHSSAKHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUV9</accession>
    <name>E3JUV9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">T-complex protein 1 subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">CCT-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_01165</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75834.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320253.2">
        <property type="nucleotide sequence ID" value="XM_003320205.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JUV9"/>
    <dbReference type="FunCoup" id="E3JUV9">
        <property type="interactions" value="800"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JUV9"/>
    <dbReference type="GeneID" id="10532749"/>
    <dbReference type="KEGG" id="pgr:PGTG_01165"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01165"/>
    <dbReference type="eggNOG" id="KOG0360">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008891_7_3_1"/>
    <dbReference type="InParanoid" id="E3JUV9"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03335">
        <property type="entry name" value="TCP1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000009">
        <property type="entry name" value="T-complex protein 1 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000070">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012715">
        <property type="entry name" value="Chap_CCT_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02340">
        <property type="entry name" value="chap_CCT_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 10" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00014424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP75834.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="558" mass="60515" checksum="69898D5AC88FB3F7" modified="2012-04-18" version="2">MAFFAKDPRTSMMLGGGQRTTGADVRQENVTAVMSVAHVLKSSLGPVGLDKMMVDDIGDMTITNDGATILSLLEVDHPAGRILVELAQQQDKEVGDGTTSVVIIAAELLKRANDLIKNKIHPTTIINGYRLACKEAIKHIQDQLAIKVDTLGKDCLINIAKTSMSSKIIGSDDDFFAQLAVDAMLAVKTINPRGEIKYPVKAVNILKAHGKSALESIYVKGYALNCTVASQAMKKGVSPAKIALLDMNLQKTRMHLGVHITIDDPEELEQIRRRESEITLEKVRMILKSGANVILTTGGIDDLCLKEFVEAGAMAVRRCKKEDLRRIARCTGGNLVSSLATLDGDEAFNPLDLGACEEVVQERISDDELILIKGTKLHSSASVILRGANDYMLDEMERALHDSLCVIKRTLESGKVVPGGGAVEAALSIYLENFATTLGSREQLAIAEFAASMLVIPKTLATNAAKDSTDLVAKLRAFHNLSQTCPKGDPKKQLKYFGLNLYEGKVVDNVKLGVIEPMMSKIRMLKSALEACTALLRIDDRIVVPKEERGGGDPHDDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3JVL7</accession>
    <name>E3JVL7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Tyrosyl-DNA phosphodiesterase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_02533</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the tyrosyl-DNA phosphodiesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76092.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320511.2">
        <property type="nucleotide sequence ID" value="XM_003320463.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JVL7"/>
    <dbReference type="STRING" id="418459.E3JVL7"/>
    <dbReference type="GeneID" id="10533496"/>
    <dbReference type="KEGG" id="pgr:PGTG_02533"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02533"/>
    <dbReference type="HOGENOM" id="CLU_007773_3_0_1"/>
    <dbReference type="InParanoid" id="E3JVL7"/>
    <dbReference type="OrthoDB" id="2497894at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="project" value="GO_Central"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Endonuclease Chain A"/>
        <property type="match status" value="2"/>
    </dbReference>
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        <property type="entry name" value="Tdp1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12415:SF0">
        <property type="entry name" value="TYROSYL-DNA PHOSPHODIESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tyr-DNA_phospho"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phospholipase D/nuclease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="88"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="51"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="89"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="224"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="452"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="454"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR610347-1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR610347-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP76092.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="573" mass="63549" checksum="BFFD60AFFA191AB8" modified="2012-04-18" version="2">MANDRPREPDGSSRNLKRTRSEVIEISSDEDGDPKQAADRHRTKIGSRDLSSIPPAASSASKPQSVLQNKPERYKLEYERLARQRARELAANPSASSSSSLSPAVTPTDQGPKDSDPSKSEPFPNYWNSAIKVTFNDWYPDCPNALRIEDIIGPKDRIKMALVSSYVLELPWIHKLFNPRTRIMVIRHHTDCGSFKVNERANMFLCHPPMLKTANGNAKPGCMHIKFFIIFYDNFCRVAIPTANAVSFDYEFVENAIWIQDFRRFSGNTIGYNSRRSDDVPPFRKTLDDLLDRMGVPLPFRKPLEDHDFRSAAANLVVSVQGTHPANSPMGQAHLAEQLKTLGLQSGPGTGRTATLECQGSSIGSYDLKWLNNFYRCASGSPPTASTEDPDLQTKTPPLSVLYPSLHTVRNSHSGKAGAGTLFCNKATWEKANFPTHIFADTMSKRTGVLMHVKMILGLFNSDSSAESTSSTLATASVDKSGARDGRINKDHAGFLYIGSHNFTPAAWGKFNSKSGSDDSTSLEISNWELGVVLPVKSHAQAEEYVTWQRPTKPYGHRGKDTSIPWMQFSHGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWU8</accession>
    <name>E3JWU8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_02964</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76523.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320942.2">
        <property type="nucleotide sequence ID" value="XM_003320894.2"/>
    </dbReference>
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    <dbReference type="GeneID" id="10528296"/>
    <dbReference type="KEGG" id="pgr:PGTG_02964"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02964"/>
    <dbReference type="HOGENOM" id="CLU_150829_1_0_1"/>
    <dbReference type="InParanoid" id="E3JWU8"/>
    <dbReference type="OrthoDB" id="2507116at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172202" evidence="2">
        <location>
            <begin position="20"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP76523.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="112" mass="12247" checksum="FD7EB768383D97BE" modified="2012-04-18" version="2">MHCLFYLACFLAVLQSALAVPTIAPRADTTATKGHSDEKCWGVYSYSCFGGGMYSFNSLLYPWSWYGASAYLPYNSLFSYSGYPGYGGLWKKDASDAGKTPSTLRSQEADTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWV5</accession>
    <name>E3JWV5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquitin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_02971</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the steroid 5-alpha reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76530.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320949.2">
        <property type="nucleotide sequence ID" value="XM_003320901.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JWV5"/>
    <dbReference type="FunCoup" id="E3JWV5">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JWV5"/>
    <dbReference type="GeneID" id="10528303"/>
    <dbReference type="KEGG" id="pgr:PGTG_02971"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02971"/>
    <dbReference type="HOGENOM" id="CLU_059260_0_0_1"/>
    <dbReference type="InParanoid" id="E3JWV5"/>
    <dbReference type="OrthoDB" id="540503at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102758">
        <property type="term" value="F:very-long-chain enoyl-CoA reductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006665">
        <property type="term" value="P:sphingolipid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="term" value="P:very long-chain fatty acid biosynthetic process"/>
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        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="3-oxo-5_a-steroid_4-DH_C"/>
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        <property type="entry name" value="SRD5A/TECR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556">
        <property type="entry name" value="3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10556:SF28">
        <property type="entry name" value="VERY-LONG-CHAIN ENOYL-COA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02544">
        <property type="entry name" value="Steroid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50244">
        <property type="entry name" value="S5A_REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="217"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="252"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="275"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="11">
        <location>
            <begin position="3"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP76530.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="330" mass="37637" checksum="F3F67FC3A1058A22" modified="2012-04-18" version="2">MTVSINLNSRSKPVKSSEIKLDKFSSFTQVCIKDIKAYIHSTYRLDPSRQRLTTADKKVLDDPQKSLGDYGLKEGDQILFKDLGPQICCLIEVLHLEDNIEMVLTFLIPFQSEYKHFGSDHPPSLTVRPIVDPPALLSIKPDLQYALWRSRQSQSITERELETILVHRFSNATMPFRNIFKNSFHYWVLSGFLLAGPIYGHASSAVKTQGSLLDSSVWLYAWSAVWAFAEISNLLVHLHLRSLRPAGSKKRSLPLGGYGFSLVCCPNYSFETLAWFSYTAMVGFHWAGCLFSLISFIQMALWSAKKLKNYRQEFKGQVPSHWKSIIPFVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3JX35</accession>
    <name>E3JX35_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02071</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76610.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003320981.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JX35"/>
    <dbReference type="GeneID" id="10528467"/>
    <dbReference type="KEGG" id="pgr:PGTG_02071"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02071"/>
    <dbReference type="HOGENOM" id="CLU_043422_0_0_1"/>
    <dbReference type="InParanoid" id="E3JX35"/>
    <dbReference type="OrthoDB" id="2507059at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00084">
        <property type="entry name" value="HMG-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="426"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="432"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76610.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="462" mass="50982" checksum="03FB532D09906BCD" modified="2012-04-18" version="2">MPPHSAVHILPPQYQDGRFQPNTADRSQDSGISHNSAGVLVIDNLENAANQILTQPNATVPAPPPTSSSLTQPLSKKRKLIAQSDESALPQSVECLLKKTYNELVVCAQENSKGSMSQADRDFFLNFYEEQRQLLAIKAIERQISMPMVDAFLGKRLALKGPNCWNQFLKTPEARAIFKEAGGVDDDSAMKKVSELWHKLTPEEKKSFAKHVDDGLTDEERALDDDPSGELDLPVETDIPIASGQVGIRATSIRSEASFRQDYQHVQAFVNQVVENATHVAATYNAQFVVFAVSNHLGPSTYQISQCTPGARVFLQYAKDVDAEHHYAARFQSHITGYSVAQIADLANKVPKRGEAKTEGVIKKWHWTDTHEKLAKAGFRLVVAPSARINPNWITQPSRQLLVDQVGLLHLDLDDKQIDIVRVPWKQAGATDRSSPRSSLGSTPEGTPPPAHSINHQQGTDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3JX88</accession>
    <name>E3JX88_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321082.1">
        <property type="nucleotide sequence ID" value="XM_003321034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JX88"/>
    <dbReference type="GeneID" id="10528601"/>
    <dbReference type="KEGG" id="pgr:PGTG_02124"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02124"/>
    <dbReference type="HOGENOM" id="CLU_050896_1_0_1"/>
    <dbReference type="InParanoid" id="E3JX88"/>
    <dbReference type="OMA" id="QEWIPRI"/>
    <dbReference type="OrthoDB" id="2511178at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="253" mass="27720" checksum="878FAD29AAB95234" modified="2011-01-11" version="1">MVETSQLNNNSESSNAENVSNDQRTEQETDLLDYSEAMTRDTPSALLQPSGFEGLGSSLPSVTGTATRPQAAETLRSEQGISPALINSRATSLALETASNRLTPVPRPVDRQVFPVNSTTVTGRSILAPTPLLPNPNRFPALSPSPAPLGLDDIIVDNQELETVIHLFNEQWNTFVQARENQNPRLMRMALVQAISSQEEIRLLAGGAEMLRICENWIAREELADLERSEAATRNQATQRLAITQTAHQHTLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXC1</accession>
    <name>E3JXC1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">hydroxyacylglutathione hydrolase</fullName>
            <ecNumber evidence="5">3.1.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glyoxalase II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_02157</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an S-(2-hydroxyacyl)glutathione + H2O = a 2-hydroxy carboxylate + glutathione + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21864"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="ChEBI" id="CHEBI:58896"/>
            <dbReference type="ChEBI" id="CHEBI:71261"/>
            <dbReference type="EC" id="3.1.2.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.6" evidence="5"/>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321115.1">
        <property type="nucleotide sequence ID" value="XM_003321067.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXC1"/>
    <dbReference type="FunCoup" id="E3JXC1">
        <property type="interactions" value="106"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JXC1"/>
    <dbReference type="GeneID" id="10528635"/>
    <dbReference type="KEGG" id="pgr:PGTG_02157"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02157"/>
    <dbReference type="eggNOG" id="KOG0813">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030571_4_0_1"/>
    <dbReference type="InParanoid" id="E3JXC1"/>
    <dbReference type="OMA" id="CKERARF"/>
    <dbReference type="OrthoDB" id="515692at2759"/>
    <dbReference type="UniPathway" id="UPA00619">
        <property type="reaction ID" value="UER00676"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004416">
        <property type="term" value="F:hydroxyacylglutathione hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019243">
        <property type="term" value="P:methylglyoxal catabolic process to D-lactate via S-lactoyl-glutathione"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07723">
        <property type="entry name" value="hydroxyacylglutathione_hydrolase_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01374">
        <property type="entry name" value="Glyoxalase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035680">
        <property type="entry name" value="Clx_II_MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032282">
        <property type="entry name" value="HAGH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017782">
        <property type="entry name" value="Hydroxyacylglutathione_Hdrlase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03413">
        <property type="entry name" value="GSH_gloB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935">
        <property type="entry name" value="BETA LACTAMASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11935:SF94">
        <property type="entry name" value="TENZING NORGAY, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16123">
        <property type="entry name" value="HAGH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="10">
        <location>
            <begin position="65"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP76696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="332" mass="36163" checksum="D25A4C33F917B67C" modified="2011-01-11" version="1">MTRLTAALFRTGFTATARGLSKAASIRSVAHQNSHLLSRRETRPLHSSPTISFKMRIVPVPVREDNYSYLIIDEKTNSAAVVDPCEPKDIIKAAKAENVTLSGTILTTHHHEDHSGGNLELISFLQENDTSKDKVKVYGGSKKIPGLTNLVEHKSNFKLGLGEKAGENDGGGIEVSCLATPCHTGDSICYYLQDSRQGGPGCVFTGDTLFVAGCGRFFEGTPEEMDQALNQRLASLPDETITYVGHEYTKSNAAFAKSIEPKNQHLLSLIKACESKDLAGITTGKYTIGDEKKFNPFMRLEEPDVASAVGLNSPSRVEVMTKLRELKNSFKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3JY77</accession>
    <name>E3JY77_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Golgi to ER traffic-protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_02463</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP77002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321421.1">
        <property type="nucleotide sequence ID" value="XM_003321373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JY77"/>
    <dbReference type="STRING" id="418459.E3JY77"/>
    <dbReference type="GeneID" id="10529269"/>
    <dbReference type="KEGG" id="pgr:PGTG_02463"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02463"/>
    <dbReference type="HOGENOM" id="CLU_035532_0_0_1"/>
    <dbReference type="InParanoid" id="E3JY77"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053094">
        <property type="entry name" value="Zinc_metalloprotease_ZmpB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193:SF2">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173061" description="Golgi to ER traffic-protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="435"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="435"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="466"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="507"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="525"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP77002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="561" mass="63213" checksum="AD0924332F242076" modified="2011-01-11" version="1">MVHLIAELTCCSLAPALLSGIWDMSKVMTSADQYQQMIKDSKCTRWDFGPELLLKVRQMIEWSKDDTYRDSFYTRKDLFRLESSTVQVLGVLSGRQYKSCLHKAVRLFQVDAEDPFWKPAVVNFKLLKRDSNRKKKTRLMRDGPTSKMISSGQSRLATLAADSYNQLHKFTVMGEPQAETGNREDDMLASMSNAGKYIIGKIRCLKAGASQTTGSNSRAGNGFLVIQRRIWETLLCILMMQQSRFLEECATLKAGHIREDEVAKLTEDMVANDRGRSIFDKAGWWHCLYNNSAYNQKDVWSLVMLAHARHEYLYTDGRYALRAPESTPWYTTDSFIRFVLRSANVTQSDPFKMDWDIAPRTFNKECNLPGRSLNLDGNPAPPMTLQEAQEEEVRQITETVRSAWDPAVAQHLITLDEAELSPDVSAADAMRGLSIDQSQPAQGTSEAQRPPESQKDKALMPPPNAQPSSAANKDNSAPPSSDRSDSPIPRRIKKKAPRQPSTPPSEPQDSSVCSGKNRQPTPTQDSDDEEEDEDEEEDEDGDESAEERNRRRKRAQDGQGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZ82</accession>
    <name>E3JZ82_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_03313</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321776.1">
        <property type="nucleotide sequence ID" value="XM_003321728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZ82"/>
    <dbReference type="GeneID" id="10538065"/>
    <dbReference type="KEGG" id="pgr:PGTG_03313"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03313"/>
    <dbReference type="HOGENOM" id="CLU_2134801_0_0_1"/>
    <dbReference type="InParanoid" id="E3JZ82"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP77357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="113" mass="13587" checksum="7F2D8991827869BF" modified="2011-01-11" version="1">MPARRKESLPKSWPYMISARRKGFLPTSWLSNQLAGRNPSRRAGLCTFFNFNTESRFWLYLMEVLEKVKWRRCLQEGYNRGKYLRMRMGMLKHQAALRSSFYVSELLDDSRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZF1</accession>
    <name>E3JZF1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcription initiation factor TFIID subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_03382</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF2 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77426.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321845.2">
        <property type="nucleotide sequence ID" value="XM_003321797.2"/>
    </dbReference>
    <dbReference type="FunCoup" id="E3JZF1">
        <property type="interactions" value="542"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JZF1"/>
    <dbReference type="GeneID" id="10538303"/>
    <dbReference type="KEGG" id="pgr:PGTG_03382"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03382"/>
    <dbReference type="eggNOG" id="KOG1474">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1932">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002317_1_0_1"/>
    <dbReference type="InParanoid" id="E3JZF1"/>
    <dbReference type="OrthoDB" id="308861at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09839">
        <property type="entry name" value="M1_like_TAF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.10">
        <property type="entry name" value="Neutral Protease Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018359">
        <property type="entry name" value="Bromodomain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057345">
        <property type="entry name" value="Ig-like_TAF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027268">
        <property type="entry name" value="Peptidase_M4/M1_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037813">
        <property type="entry name" value="TAF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057991">
        <property type="entry name" value="TPR_TAF2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15137">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15137:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25316">
        <property type="entry name" value="TAF2_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25577">
        <property type="entry name" value="TPR_TAF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00297">
        <property type="entry name" value="BROMO"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00633">
        <property type="entry name" value="BROMODOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromo" evidence="10">
        <location>
            <begin position="1216"/>
            <end position="1288"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="10">
        <location>
            <begin position="1825"/>
            <end position="1897"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="10">
        <location>
            <begin position="1933"/>
            <end position="2008"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="121"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="622"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1155"/>
            <end position="1175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1363"/>
            <end position="1454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1468"/>
            <end position="1658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="2028"/>
            <end position="2059"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="126"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="631"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="1369"/>
            <end position="1378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1399"/>
            <end position="1408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1430"/>
            <end position="1448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="1488"/>
            <end position="1507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="1589"/>
            <end position="1599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP77426.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="2059" mass="229482" checksum="B387F4850180CD14" modified="2012-04-18" version="2">MSSHGPQNRAFSVTHQRAILDIKLDGTINAITEITILPLSADLNTVHLHSSHCTISSIYHPSQHPNNTHQHKQPLHFILNQPNLVRIPDPNSVRQFPEAKRRLFEQVNEKETGELAIQIDRHKINRTSNRQSATSLLPPSDQHQLSAQKDKDLEFDPIIITIEYQITQADLTLNPAICVVGGQHPHLFTNSISARSWVPCLDSLWERSPWELEFIVPRSSITNHAKPSPLTVVASGELIEQIIHPTDPSKTVFYYAQAIPTSVQHIAWVVGPLTVNDLTPYRPPPPSSPHQDKADQPVDQKIITDQHHQSSTEINLLAFCLPNRQSQLLHVVNFLPHALSFFTQEYGSYPYSSYKLVFLDMSAATHSICATFNSATLTLCPSDLLYPPQVIDQVYETKPVLVQSLASQWIGINIIPKSPSDTWLINGLSLYITGLYFKVIWGTNDYRYRIKQEIMKCVEMDVDKPPICHLDCFIDRHRSLGLCQPQSKSRTLHPRSTSQAIGRWHISRLDFLRICRKLNGNELKSWADQWIYASGCPIFQVTAYFNRKKLTHELTLRQLNRSKQYYEDDQTSWAEKCNLKPVDRFEGQMSIRIQESDGIPYEHVLDIKEPFKRYELPSNHKNKKFKRSSRRYASSNQAPDLTETSTETFTYPPWERSQEERDRWKVEDWTEEEDLFITQSNVEWIRLDVEVEWICEFRFEMKEFMWLEQLQRDRDVVAQLEAVRALKTLPSKVVSSHLCRVVLISEYFFRIRIEAILALVSCATSRCGFLGLFHLLKIFQSRFCYPPQVEPDSPWHIRAIPKPNQFDNFADYFIRKALVVALSHVRDQNNNSPSVCHQFFLDQLVYNDNSLNPYSDAHYIATTIGSMTDSLIHLADNAGREQLVEIFGTKALSTGTNGDLEKTCDKRLLLMINELERCFMADRLVPSYRNLIAMAVIEAKLKLMFAGLLPVDLVFFLLCARPGNYLPLRILAFDCLLLLRGFQEKAIIRYMFMVLRDDSSLLVRRRLAMGLVQCLPILVLIEELGMEPSDQNFSVIDESHPLNTQKFDHKTVTKHLRAEVGRALILRECIMSVMLHPTVDTDVQTCLLKISEVLFKPSDESVPVRIPEEKQPPVEAPVPVATKIRIHAPSVPPIEPSQSPAPLPKIILKDHQQHLANDTDPGGSSPPRLPPPDVPSKLAAALIRKPKPPPKPKKFQASGMTIPDHKMCQNLLKKLASNPLSQPFRKPVDPIRESAPNYFNIISNPMDFKTMGLKLEMGQYPNREAFKDDVNLIFKNCKTYNLPDSSLVVKYAEPLKATFDKLWERSEKTMSAIQAKGLGGVNPGRLQPTDAKSIVAPSNVVPLAPPPSFAAFAAGGSMIPPVSSSPISPGLPPPPPVPSRMSPEASRPGRLKVKLKPRTSISTGSTTSEILPENNSSIPPAPKSGLKIKFTTTATNSTLANPPSTPTTVKPEGGVCMSSTYPSIPFPASSSSTYPPPPPPIISMAPPKSRPPPTPSFVPPPPPPPPTFESFSLAAQLGPKPTPAPTRTEKPSVEVETSDPFPNLLEKPTKSKKRPSSSQPTSIAAPPPPPGSSSSQKPVKRNNLIVQFPKPPALPPPSAPAVITPSSAPGPTIGLPNFPAPPPPSLPKLDLKSSKHPTPPTSSIPPAKSTVNPPLSSNPIKIKVVQRPAPRPVVQDRPESSVPSDVANFLQTIKQSWRRVQNQRKIDGYQECDCFLLKHIWNVSHPIQLTPLSRGSTSTSTSAVSHWSGSRKTKKVDLYRSTSSKAQDIGLLHLSPSTSTAAGKSVTLAPPPSSSVVSEPVKAASPVADDLLNPKKAKVIIRKMMEHPQGVWFRIPVDPIAVGAPTYLDEIKNPMDLSTMLKKLDKGTYKRHSELMADFELIVSNCVQFNGAESQLGLEAKALKKVWDLEWEKAAKMSYTDKRSLLGLFNKLNQVPGVELFNEPVDPVKYQIPTYFAVIGGQQNARDLGTIKANLVADRYNSIGEFEADVRLMLKNCFTFNAPTTPVEVIGRDLEKAFDLAMVKVRKDAGLPPPAGSANAGNKRKGAEPAASSKRSRVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZM0</accession>
    <name>E3JZM0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_03451</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321914.1">
        <property type="nucleotide sequence ID" value="XM_003321866.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZM0"/>
    <dbReference type="GeneID" id="10538533"/>
    <dbReference type="KEGG" id="pgr:PGTG_03451"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03451"/>
    <dbReference type="HOGENOM" id="CLU_051119_2_0_1"/>
    <dbReference type="InParanoid" id="E3JZM0"/>
    <dbReference type="OrthoDB" id="2498930at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027532">
        <property type="entry name" value="Mdm12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204:SF1">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="196"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP77495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="289" mass="32702" checksum="81A012653FF0F876" modified="2011-01-11" version="1">MSTEVIRETKVLLTPNNYALWLLPMEARLFRLKLLDILEGQVEPDEKEKENWTKRNNDAYAEIIAYIGQEVLGFVSSALPATPRFNGRGLWKLLKSQYAGDDLTSQTTGLDQFLHLSFSSIATFIPAVRSANQKISMSGLVLDDRVRTILMLKKLPSDYRSFRDIVSMNYGSESFESVIKKLESYVAQNDLDKKPSSTSLTSPQSLLTRSNDQASASSTVCDHCKKPGHRVNNCWVKYPEKAPKSHSAHLTVQDNFNQLSDPTDFSLFRTADGVLHHIDEVKYEGVRYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZP9</accession>
    <name>E3JZP9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Matrin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_03480</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321943.1">
        <property type="nucleotide sequence ID" value="XM_003321895.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZP9"/>
    <dbReference type="STRING" id="418459.E3JZP9"/>
    <dbReference type="GeneID" id="10538563"/>
    <dbReference type="KEGG" id="pgr:PGTG_03480"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03480"/>
    <dbReference type="eggNOG" id="KOG0150">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_883021_0_0_1"/>
    <dbReference type="InParanoid" id="E3JZP9"/>
    <dbReference type="OMA" id="VEPPVFF"/>
    <dbReference type="OrthoDB" id="191651at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000690">
        <property type="entry name" value="Matrin/U1-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013085">
        <property type="entry name" value="U1-CZ_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040023">
        <property type="entry name" value="WBP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13173">
        <property type="entry name" value="WW DOMAIN BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13173:SF10">
        <property type="entry name" value="WW DOMAIN-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06220">
        <property type="entry name" value="zf-U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50171">
        <property type="entry name" value="ZF_MATRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Matrin-type" evidence="7">
        <location>
            <begin position="11"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="79"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="106"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="167"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="183"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="227"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="289"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP77524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="323" mass="37016" checksum="F014A749D2E7DF5A" modified="2011-01-11" version="1">MTEYWVSKQSYYCKYCDIFIRDDKPSRAQHENGLRHKGNLERYIRDIYKKEDRAKKERTDEASQVSKIEKAARLAHQQKDLALVEGEKEVKEETEEGDEPRKRPKYDKQDWKGAEDLQNYSDARSLGFIPDTDSEAARALKEEEEAEAEARSKEGRMGQWEAVLKIQPSNRAGSSASTQAPISREKIVSQLTHDRDPDNKPIRKLFSERNLEAEPDTEILEGMQIKVKVDRRAKAQEDRSLSGSRIHGSLQPIRLDGLQSNQTEPIKSEESSPSAPDPSFKNDLAQEVEDVKPDVDDLKLPTPQPPVFFKKRKATQAQPRQKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZV1</accession>
    <name>E3JZV1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_03532</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77576.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321995.2">
        <property type="nucleotide sequence ID" value="XM_003321947.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZV1"/>
    <dbReference type="STRING" id="418459.E3JZV1"/>
    <dbReference type="GeneID" id="10538783"/>
    <dbReference type="KEGG" id="pgr:PGTG_03532"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03532"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_476621_0_0_1"/>
    <dbReference type="InParanoid" id="E3JZV1"/>
    <dbReference type="OrthoDB" id="1405595at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051059">
        <property type="entry name" value="VerF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626">
        <property type="entry name" value="MIP31509P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40626:SF11">
        <property type="entry name" value="ZINC FINGER PROTEIN YPR022C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="8"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP77576.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="572" mass="63940" checksum="6DE0E811D480ECD3" modified="2012-04-18" version="2">MVHKTGVHKCPECPKTFSRLEYVTRHRRVKHLGARPWSCQCGVSYARSDLLTRHKRKCSQAKESIGASSKYNLDDTSASSVVTSVNHSSIIKASDHSSDDNQPPTYQQAISDQAPFTANTNHPTDHLAQASSFRADLARDTRFSLPRPDGLISQEADNFGPGTTLRQFSLNCTAEEGLSSLVPPDDQEFYDVARENTNQREIENIPEVSSDVGTSIVPLVRFSDPSFLISDVPQSSPFYLNPSIWILAFLCQKSQGFTIPHMGSLSAYLSRATEVMCPVIPMFHVPTLRATQISIHLGYALSVSGAALESSEHALAFTYQSLSYKRISVQNDFLRRDRSFTFRFELLQSLLTYQFLGMFSRLEVQGNVPSDSARPLYRELAFIETLRNSPDYVQLALSGQVPLEVAWKLWVEYEVQKRTAFLVLISALQLRGPYESGLRLSETNLPLPCHEEIWTARSSEEWAQAARKHAMCCWLDNMQTSESFVDHTNIQQVVGEQSIPLLSEALAALHISDSTSQINQSIPDIHRVGASRLTVRAERLGCFAKTVIHHARALTHQATVSIHLLEPPPLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3K0V3</accession>
    <name>E3K0V3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03884</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP77928.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322347.2">
        <property type="nucleotide sequence ID" value="XM_003322299.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K0V3"/>
    <dbReference type="GeneID" id="10541075"/>
    <dbReference type="KEGG" id="pgr:PGTG_03884"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03884"/>
    <dbReference type="HOGENOM" id="CLU_1289503_0_0_1"/>
    <dbReference type="InParanoid" id="E3K0V3"/>
    <dbReference type="OrthoDB" id="10422489at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77928.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="214" mass="23216" checksum="ED450E17029E0A37" modified="2012-04-18" version="2">MVKPSHLEGGDEWESRSTGGSLDIRSSTYSGHLNRRAKGGVANGDSRLKNEIEWKEIPHSTNEPVLPPPAHQQVSSDSSESSESARRKQKYRQRTPRLDDEDDDPIDPHLVRSHPPASSGSSTLIIAANSLLPPDTPPSSIETLASKAIGSSSSISLFLIPLLPPILNFLQIIMGVFPPSMIGFDRSSMGVFTSIKDRLKPVLDGGVSPIDDRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3K1I8</accession>
    <name>E3K1I8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Ubiquitin-like protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_04119</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP78163.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322582.2">
        <property type="nucleotide sequence ID" value="XM_003322534.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K1I8"/>
    <dbReference type="SMR" id="E3K1I8"/>
    <dbReference type="FunCoup" id="E3K1I8">
        <property type="interactions" value="556"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K1I8"/>
    <dbReference type="GeneID" id="10541793"/>
    <dbReference type="KEGG" id="pgr:PGTG_04119"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04119"/>
    <dbReference type="eggNOG" id="KOG0005">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010412_6_4_1"/>
    <dbReference type="InParanoid" id="E3K1I8"/>
    <dbReference type="OrthoDB" id="419317at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01806">
        <property type="entry name" value="Ubl_NEDD8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000023">
        <property type="entry name" value="NEDD8 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038738">
        <property type="entry name" value="Nedd8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000626">
        <property type="entry name" value="Ubiquitin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019954">
        <property type="entry name" value="Ubiquitin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019956">
        <property type="entry name" value="Ubiquitin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050158">
        <property type="entry name" value="Ubiquitin_ubiquitin-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
        <property type="entry name" value="UBIQUITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00348">
        <property type="entry name" value="UBIQUITIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00213">
        <property type="entry name" value="UBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
        <property type="entry name" value="UBIQUITIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin-like" evidence="2">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP78163.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="77" mass="8515" checksum="3D7F70948CC52B80" modified="2012-04-18" version="2">MQIKVKTLTGKEIELDVESDDKISKVKDRIEEKEGIPPSQQRLIFGGKQMPDEKTVKELGIEGGSTLHLVLALRGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3K219</accession>
    <name>E3K219_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04344</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322807.1">
        <property type="nucleotide sequence ID" value="XM_003322759.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K219"/>
    <dbReference type="GeneID" id="10535986"/>
    <dbReference type="KEGG" id="pgr:PGTG_04344"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04344"/>
    <dbReference type="HOGENOM" id="CLU_080784_0_0_1"/>
    <dbReference type="InParanoid" id="E3K219"/>
    <dbReference type="OrthoDB" id="2510958at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="300" mass="33701" checksum="E1BF476057DBD8E5" modified="2011-01-11" version="1">MELSLTREAKAVILVHLKKPTLRPKNLHPILKQRKGKENARLIKKNVGFAQLRAPCVHYPDFLALLTAGLMVRRDTRRFRPFPTQRSWRLRRPVYIDLEREDTPEPRAGAPHDAGRHISEPVVVERAHEELMAAGQLPVANPNIQPPESPTNTTRANSNLAVNLPGSLAPPNADSNSNHLHVFEGNNPRRMPPAPTLAQPNANPFVARVPFPPARAAMEAVAMETYLNIAHIPRDDLRTRSRLLVHGIGHWTFFRATNEAELTRLGFPLGIARLLCEGVARLEAFREEMEREGIEMSPIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2L4</accession>
    <name>E3K2L4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04539</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323002.1">
        <property type="nucleotide sequence ID" value="XM_003322954.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2L4"/>
    <dbReference type="GeneID" id="10536348"/>
    <dbReference type="KEGG" id="pgr:PGTG_04539"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04539"/>
    <dbReference type="HOGENOM" id="CLU_169161_0_0_1"/>
    <dbReference type="InParanoid" id="E3K2L4"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172346" evidence="1">
        <location>
            <begin position="24"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="117" mass="13250" checksum="BA18CF3427B88CD9" modified="2011-01-11" version="1">MQFSDFFKTLSVVLLHLTVPCAASNPRYFLCGHGEYKYCAYKDYSTQPMKYKFGPASQVTREGRSTCSVSNLSSKSIIDVCCDNPVGLLKPRRFGTELTVSDNDYKHRFKCREASPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="99" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2M0</accession>
    <name>E3K2M0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Pentafunctional AROM polypeptide</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="20">3-dehydroquinate synthase</fullName>
                <shortName evidence="20">DHQS</shortName>
                <ecNumber evidence="20">4.2.3.4</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="20">3-phosphoshikimate 1-carboxyvinyltransferase</fullName>
                <ecNumber evidence="20">2.5.1.19</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="20">5-enolpyruvylshikimate-3-phosphate synthase</fullName>
                <shortName evidence="20">EPSP synthase</shortName>
                <shortName evidence="20">EPSPS</shortName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="20">Shikimate kinase</fullName>
                <shortName evidence="20">SK</shortName>
                <ecNumber evidence="20">2.7.1.71</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="20">3-dehydroquinate dehydratase</fullName>
                <shortName evidence="20">3-dehydroquinase</shortName>
                <ecNumber evidence="20">4.2.1.10</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="20">Shikimate dehydrogenase</fullName>
                <ecNumber evidence="20">1.1.1.25</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="28" type="ORF">PGTG_04545</name>
    </gene>
    <organism evidence="28 29">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="29" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="29">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="20 21">The AROM polypeptide catalyzes 5 consecutive enzymatic reactions in prechorismate polyaromatic amino acid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20 21">
            <text>3-dehydroquinate = 3-dehydroshikimate + H2O</text>
            <dbReference type="Rhea" id="RHEA:21096"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="EC" id="4.2.1.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21256"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="EC" id="2.5.1.19"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="18">
            <dbReference type="Rhea" id="RHEA:21257"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20 21">
            <text>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate = 3-dehydroquinate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21968"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="EC" id="4.2.3.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19 20 21">
            <text>shikimate + ATP = 3-phosphoshikimate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13121"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.71"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20 21">
            <text>shikimate + NADP(+) = 3-dehydroshikimate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17737"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="20 21">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="20 21">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="20 21">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 2/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="20 21">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="20 21">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 20 21">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 20 21">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="20 21">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="20 21">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the EPSP synthase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="20 21">In the 2nd section; belongs to the EPSP synthase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the 2nd section; belongs to the type-I 3-dehydroquinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="20 21">In the 3rd section; belongs to the shikimate kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="20 21">In the 4th section; belongs to the type-I 3-dehydroquinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="20 21">In the C-terminal section; belongs to the shikimate dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="21">In the N-terminal section; belongs to the dehydroquinate synthase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the shikimate kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="20">In the N-terminal section; belongs to the sugar phosphate cyclases superfamily. Dehydroquinate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.4" evidence="20"/>
    <dbReference type="EC" id="2.5.1.19" evidence="20"/>
    <dbReference type="EC" id="2.7.1.71" evidence="20"/>
    <dbReference type="EC" id="4.2.1.10" evidence="20"/>
    <dbReference type="EC" id="1.1.1.25" evidence="20"/>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323008.1">
        <property type="nucleotide sequence ID" value="XM_003322960.2"/>
    </dbReference>
    <dbReference type="SMR" id="E3K2M0"/>
    <dbReference type="FunCoup" id="E3K2M0">
        <property type="interactions" value="103"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K2M0"/>
    <dbReference type="GeneID" id="10536354"/>
    <dbReference type="KEGG" id="pgr:PGTG_04545"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04545"/>
    <dbReference type="HOGENOM" id="CLU_001201_1_2_1"/>
    <dbReference type="InParanoid" id="E3K2M0"/>
    <dbReference type="OMA" id="WEQISAP"/>
    <dbReference type="OrthoDB" id="197068at2759"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00085"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003855">
        <property type="term" value="F:3-dehydroquinate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003856">
        <property type="term" value="F:3-dehydroquinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003866">
        <property type="term" value="F:3-phosphoshikimate 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004764">
        <property type="term" value="F:shikimate 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004765">
        <property type="term" value="F:shikimate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00502">
        <property type="entry name" value="DHQase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08195">
        <property type="entry name" value="DHQS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01556">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01065">
        <property type="entry name" value="NAD_bind_Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00464">
        <property type="entry name" value="SK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1090.10:FF:000007">
        <property type="entry name" value="Pentafunctional AROM polypeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000135">
        <property type="entry name" value="Pentafunctional AROM polypeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:004059">
        <property type="entry name" value="Pentafunctional AROM polypeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.65.10.10:FF:000012">
        <property type="entry name" value="Pentafunctional AROM polypeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1090.10">
        <property type="entry name" value="Dehydroquinate synthase-like - alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="entry name" value="Enolpyruvate transferase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00210">
        <property type="entry name" value="EPSP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03143">
        <property type="entry name" value="Pentafunct_AroM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00109">
        <property type="entry name" value="Shikimate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018508">
        <property type="entry name" value="3-dehydroquinate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030960">
        <property type="entry name" value="DHQS/DOIS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056179">
        <property type="entry name" value="DHQS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001381">
        <property type="entry name" value="DHquinase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006264">
        <property type="entry name" value="EPSP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023193">
        <property type="entry name" value="EPSP_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008289">
        <property type="entry name" value="Pentafunct_AroM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041121">
        <property type="entry name" value="SDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031322">
        <property type="entry name" value="Shikimate/glucono_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013708">
        <property type="entry name" value="Shikimate_DH-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000623">
        <property type="entry name" value="Shikimate_kinase/TSH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023000">
        <property type="entry name" value="Shikimate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01356">
        <property type="entry name" value="aroA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01093">
        <property type="entry name" value="aroD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21090">
        <property type="entry name" value="AROM/DEHYDROQUINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21090:SF5">
        <property type="entry name" value="PENTAFUNCTIONAL AROM POLYPEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01761">
        <property type="entry name" value="DHQ_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24621">
        <property type="entry name" value="DHQS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01487">
        <property type="entry name" value="DHquinase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18317">
        <property type="entry name" value="SDH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08501">
        <property type="entry name" value="Shikimate_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01202">
        <property type="entry name" value="SKI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000514">
        <property type="entry name" value="Pentafunct_AroM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01100">
        <property type="entry name" value="SHIKIMTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56796">
        <property type="entry name" value="Dehydroquinate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01028">
        <property type="entry name" value="DEHYDROQUINASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00104">
        <property type="entry name" value="EPSP_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00885">
        <property type="entry name" value="EPSP_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01128">
        <property type="entry name" value="SHIKIMATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 20 21" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="16 20 21" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="13 20" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 20 21" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 20 21" id="KW-0418">Kinase</keyword>
    <keyword evidence="17 20 21" id="KW-0456">Lyase</keyword>
    <keyword evidence="9 20 21" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="14 20" id="KW-0521">NADP</keyword>
    <keyword evidence="10 20" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15 20 21" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="29" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 20 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 20 21" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="3-dehydroquinate synthase N-terminal" evidence="24">
        <location>
            <begin position="3"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="3-dehydroquinate synthase C-terminal" evidence="27">
        <location>
            <begin position="46"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Enolpyruvate transferase" evidence="22">
        <location>
            <begin position="274"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="domain" description="Shikimate dehydrogenase substrate binding N-terminal" evidence="25">
        <location>
            <begin position="1182"/>
            <end position="1265"/>
        </location>
    </feature>
    <feature type="domain" description="Quinate/shikimate 5-dehydrogenase/glutamyl-tRNA reductase" evidence="23">
        <location>
            <begin position="1311"/>
            <end position="1358"/>
        </location>
    </feature>
    <feature type="domain" description="SDH C-terminal" evidence="26">
        <location>
            <begin position="1446"/>
            <end position="1465"/>
        </location>
    </feature>
    <feature type="region of interest" description="3-dehydroquinate synthase" evidence="20">
        <location>
            <begin position="1"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Shikimate dehydrogenase" evidence="20">
        <location>
            <begin position="1177"/>
            <end position="1482"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for 3-dehydroquinate synthase activity" evidence="20">
        <location>
            <position position="124"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for 3-dehydroquinate synthase activity" evidence="20">
        <location>
            <position position="139"/>
        </location>
    </feature>
    <feature type="active site" description="For EPSP synthase activity" evidence="20">
        <location>
            <position position="684"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for 3-dehydroquinate dehydratase activity" evidence="20">
        <location>
            <position position="1063"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; for 3-dehydroquinate dehydratase activity" evidence="20">
        <location>
            <position position="1091"/>
        </location>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="34"/>
            <end position="37"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="128"/>
            <end position="132"/>
        </location>
        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="151"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <begin position="736"/>
            <end position="743"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00048567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="PIRNR" id="PIRNR000514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF01488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF01761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF08501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF18317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF24621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="EMBL" id="EFP78589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1482" mass="161128" checksum="5593BB4EBBD163DB" modified="2011-01-11" version="1">MATFGSLTAIDTPLGKNLVGAFWQPSFIFIDASYLETLPEREFVNGMAEMIKTAAIWDESQFEKLESNVDSIRSAVLSPPPRDPINPFPGRDLASRSQAQKLLLEVIADSVGVKSEVVTKDEKETGLRNLVNFGHTIGHAIEAVLTPAILHGECVSIGMILEAEVARARGCLSSSAIARLSKCLKAHGLPVSLTDPRILSSPNAPNLNLDRLLDLMSVDKKNEGKVKKIVLLSRIGKTLEERATGVEDSLIRRVLAPAVRVHPGPPPANIKEITIRTPGSKSISNRALILAALGNGTCRLKNLLHSDDTQVMINALEEMKGASFSWEDNGATLVVSGGAGKLSSPANGKHVYLGNAGTAARFLTTVCSLVKSQVSNQSSTVITGNARMQERPIGPLVDTLRTNGVQIDYLRNEGCLPLRISPEDGFPGGMIELAASVSSQYVSSVLLSAPFAQAPVTLSLVGVERVKDPATGLPSNTYRIPNGTYKNPPVYEIESDASSATYPLAMAALNGLSITLETIGSGSLQGDAQFAKKVLEPMGCQVIQTERETKVIGPSTVSELRQLGEIDMEEMTDAFLTACVVLGVAVQPSEKEQKMSTRIIGIANQRVKECNRIAAMVAELGKMGIHAQELEDGIEVFGTPVDALAKRGDQVRINCYDDHRIAMAFSVLGTVPGGKGLILNEKRCVEKTWPSWWDDLSTKLGIQLDGVPAHEKLTNTTSAVAWEAKKPETPSILLCGMRGSGKSFSGQVAASTLGWPLIDTDLYFQEKCGCSIREFIQKNDWSRFREEECAVLEEILKEFPSCHVISLGGGIVETEKGRKMLMNYANSKGPVVEIIRPVEEVIAYLNEDGNRPSLGESIQQIWARREPWYRLCSNSEYFNLVHPAVDHALISEVQHAKRAMQQFFRFITGVDTNHVLLDPPVPRNTHFLCLTFPSLSPCREEDKTSLDRFEELTIGCDAVELRVDLLSTSGKAPTSPSIPPTEFVVKQVAALRRLTTLPIIYTVRTCSQGGMFPDGQPEEWCKLAAMGFRTGCEYVDIELGLSPEQQKALCSQKGHSKIIASYHDFSGRLKWDSSEMIQRYHELKAYGDMVKLVSRAADGLEDNLAMLEFRKKHATLQKDRQSLITINMGRSGQMSRILSPVLSPTTHRLLPGPPAAPGQLSFRQIQTAQGLLGQIIPKQFWLFGAPIGQSRSPLIHGTGFDTLGLEGYRYQKCETSSVTDSTVLEKIHASDFGGASVTIPLKVEIIKYLDQLTPDAQAVGAVNTIVPVSQRDGKIKLLGANTDWEAIKMKIEANLPAQQALGLITPGQLVRAAGVVIGAGGTARAAVYALHSLGYEQIYIHNRTRSKAEEVAQAFPSYFGIRVIDSLGFLCSGEDGVWQPSAIISAVPAQATRLSDDPPADKLEIPHGLFDRPGGGVVIEMSYALGKDSALLRAVQDLVRWKSVDGLQVLVQQAARQFQLWTGKHLAMKIVSQAVYQEASSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2N2</accession>
    <name>E3K2N2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Extra spindle pole bodies 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04557</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323020.1">
        <property type="nucleotide sequence ID" value="XM_003322972.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2N2"/>
    <dbReference type="GeneID" id="10536450"/>
    <dbReference type="KEGG" id="pgr:PGTG_04557"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04557"/>
    <dbReference type="HOGENOM" id="CLU_1475841_0_0_1"/>
    <dbReference type="InParanoid" id="E3K2N2"/>
    <dbReference type="OrthoDB" id="10268364at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="20914" checksum="E6BBCCCEDE164CD4" modified="2011-01-11" version="1">MHDTEYSFTSIQYNIIKKHLNWGATDNSRDGRRVSVADTRYLLADTRQRVQIRVRMAPFPQKVGGYPGIPKDICGQKGRWLAGRRPFQPARYKYLVDWKDTLPVDEVLVPCRMEGHPSGQRGTCTSLTGRTPFRSTRYLYLAGWKEALPVDEVQVPRQLEGDPSSHQGTSTLPAGRRPFQPVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2W2</accession>
    <name>E3K2W2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Vacuolar protein sorting-associated protein 17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_04238</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322701.1">
        <property type="nucleotide sequence ID" value="XM_003322653.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2W2"/>
    <dbReference type="FunCoup" id="E3K2W2">
        <property type="interactions" value="39"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K2W2"/>
    <dbReference type="GeneID" id="10542243"/>
    <dbReference type="KEGG" id="pgr:PGTG_04238"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04238"/>
    <dbReference type="eggNOG" id="KOG2273">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_399079_0_0_1"/>
    <dbReference type="InParanoid" id="E3K2W2"/>
    <dbReference type="OMA" id="FYLGTME"/>
    <dbReference type="OrthoDB" id="9976382at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005768">
        <property type="term" value="C:endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030905">
        <property type="term" value="C:retromer, tubulation complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032266">
        <property type="term" value="F:phosphatidylinositol-3-phosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001683">
        <property type="entry name" value="PX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053055">
        <property type="entry name" value="VPS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015404">
        <property type="entry name" value="Vps5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47433">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47433:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00787">
        <property type="entry name" value="PX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09325">
        <property type="entry name" value="Vps5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64268">
        <property type="entry name" value="PX domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX" evidence="3">
        <location>
            <begin position="222"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Sorting nexin/Vps5-like C-terminal" evidence="4">
        <location>
            <begin position="350"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="88"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="581"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="485"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="8"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="94"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="106"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="133"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="168"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="610"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="623"/>
            <end position="637"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP78282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="690" mass="78957" checksum="9D6C70AD97AD7F02" modified="2011-01-11" version="1">MDPLGSINTNTNNNNSNNNNRPNPFSSNQQQQQQQQQHYHQQQQQQQHHYHQTPDKRYYPKSPNTSWPSSPLIKPNLVGAASANQQPIIWPARSNPTTPTITQLNREPRSPIREEPRRTLNNQQPIPQHSEHTQQQQHQQQQQKQQQHQHQPYQQQQQQQHHHQQQQYPNISQNNHQNTPSENIRPEKRQAKIRVTGIERNRRDLYIKFDAWSNLTTNRTGHCPNVSRAYKEFLSFHEALICNNPHTIVPPIPFPNTSAPSPADDDRLVASAFQQFFDRLLINNFLRNDDELRVFLESDFGYTPQTKIKKRSITSGSGWLSKPAVGSSVRTLPSSTIQQGPGGTPDTVVSIVEEDELSNAKLQFRLLEDRLVDCCRSVEQLSRSRRSLAQALLDLSGRVTQFSTTESHGALAEGFRRLGQTIRAISELETNSAVGYLVTLSDGLSWCSVSAKAAKDVLRNRAGVLHEHYTSVKKSIEKRRLIERIKSTNVSSERVESALEELEEAKKAEETIAQKAMLISTNLRPSLRSHSELLHDDLLYSLMENARTQLIYEKQILKELERVKPELDSISLRPANVIYGHSSTNLESDSHHHHHHHRHQQDYQNNNNYIRRDSSADHPNHRASLSSSTYSNHQAQHSSTIINHHHIQPTPIRTGEISKKLVVHDRLGENVDCRQRVDAKSAAASLARLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>E3K364</accession>
    <name>E3K364_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">polynucleotide adenylyltransferase</fullName>
            <ecNumber evidence="5">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_04877</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
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            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <cofactor evidence="2">
            <name>Mg(2+)</name>
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            <name>Mn(2+)</name>
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            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
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        <text evidence="4">Belongs to the DNA polymerase type-B-like family.</text>
    </comment>
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        <property type="term" value="F:nucleotidyltransferase activity"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="POLY(A) RNA POLYMERASE GLD2"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3K397</accession>
    <name>E3K397_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP78954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003323325.2"/>
    </dbReference>
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    <dbReference type="GeneID" id="10537142"/>
    <dbReference type="KEGG" id="pgr:PGTG_04910"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04910"/>
    <dbReference type="HOGENOM" id="CLU_1714193_0_0_1"/>
    <dbReference type="InParanoid" id="E3K397"/>
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    <dbReference type="Proteomes" id="UP000008783">
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="153" mass="17858" checksum="F5112BFC2B61AB3C" modified="2011-01-11" version="1">MVHPVMEAARRKLVRKLVHRTMVKDKKKSKSKSKSRSRPSQSTNHVMAFVDPQAKKRAWQEMIRQYSNGLIKARHPRVLTLELQRKEKVHKPRGTPARISNHSQGEFLRRSTRQPHPRNPPLDPTRDQLIAVPRSEMSDEDDDDGFLIERDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3H2</accession>
    <name>E3K3H2_PUCGT</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
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    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP79029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3H2"/>
    <dbReference type="GeneID" id="10530850"/>
    <dbReference type="KEGG" id="pgr:PGTG_04985"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04985"/>
    <dbReference type="eggNOG" id="ENOG502S85Z">
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    </dbReference>
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    <dbReference type="InParanoid" id="E3K3H2"/>
    <dbReference type="OMA" id="PKLGQHY"/>
    <dbReference type="OrthoDB" id="2495471at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021851">
        <property type="entry name" value="DUF3455"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35567:SF1">
        <property type="entry name" value="CONSERVED FUNGAL PROTEIN (AFU_ORTHOLOGUE AFUA_1G14230)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35567">
        <property type="entry name" value="MALATE DEHYDROGENASE (AFU_ORTHOLOGUE AFUA_2G13800)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11937">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    <feature type="signal peptide" evidence="1">
        <location>
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            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172986" evidence="1">
        <location>
            <begin position="21"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="209" mass="23373" checksum="F8CE941BF5B3D5EE" modified="2011-01-11" version="1">MISAFILHFLCFQIFTVAAGTNTVNDFQFHMPKGSQLQPPVGLKLKFLALGNGTQNYMCKKDPASGNALTWMPHGADATLFDISKDSSKAASLATDVMRGGKNHQAKPNLKAYPVLGKHFFTPTKPDMTLMPTFEINHMKIILRKARFFPSPLNSKRNVDWLQLNTIQGNFATRVYRTYTRGGKVPAPQCNTVNQIIKEPYAAIYSFWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>E3K496</accession>
    <name>E3K496_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_05427</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79106.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323525.2">
        <property type="nucleotide sequence ID" value="XM_003323477.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K496"/>
    <dbReference type="GeneID" id="10530929"/>
    <dbReference type="KEGG" id="pgr:PGTG_05427"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05427"/>
    <dbReference type="HOGENOM" id="CLU_2723403_0_0_1"/>
    <dbReference type="InParanoid" id="E3K496"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP79106.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="72" mass="8307" checksum="8F4A57D4606D8B8B" modified="2012-04-18" version="2">MATPVGHLFQVWVAKCGIATQFSDPRGDALMTEMAKEIYWKTSRQHLRVSQSRFRASKFKFPFKNQESLGLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3K5N9</accession>
    <name>E3K5N9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06113</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_003324163.2"/>
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    <dbReference type="AlphaFoldDB" id="E3K5N9"/>
    <dbReference type="STRING" id="418459.E3K5N9"/>
    <dbReference type="GeneID" id="10533317"/>
    <dbReference type="KEGG" id="pgr:PGTG_06113"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06113"/>
    <dbReference type="HOGENOM" id="CLU_003292_5_1_1"/>
    <dbReference type="InParanoid" id="E3K5N9"/>
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    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="entry name" value="Phage_Integrase-like_Recomb"/>
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        <property type="entry name" value="P CELL-TYPE AGGLUTINATION PROTEIN MAP4-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PHAGE_INTEGRASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79792.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="162" mass="18284" checksum="D5167305FDEAF7E3" modified="2012-04-18" version="2">MARIGEFTYDKTFGDLNAENAVLTSDVRLVKSAIGEKAIITFKKHALPVLALKQRLDEANGENTSLFGFYREGTRRHLTRSIAVNRIQLVLRAGGFEGLLGHSFRVGGASLRYALGTPIEEICLLGRWISNCYRLYIREYTAEDTEDSKKIIAELNDLWEED</sequence>
</entry>
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    <accession>E3K7H4</accession>
    <name>E3K7H4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_06244</name>
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    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
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    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80288.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324707.2">
        <property type="nucleotide sequence ID" value="XM_003324659.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K7H4"/>
    <dbReference type="GeneID" id="10539295"/>
    <dbReference type="KEGG" id="pgr:PGTG_06244"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06244"/>
    <dbReference type="HOGENOM" id="CLU_1005210_0_0_1"/>
    <dbReference type="InParanoid" id="E3K7H4"/>
    <dbReference type="OrthoDB" id="2509396at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173293" evidence="3">
        <location>
            <begin position="23"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
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            <begin position="95"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="244"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP80288.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="277" mass="30718" checksum="E9A43AF3E7E05484" modified="2012-04-18" version="2">MVSLFLSVGLLLFFQKFYQFSAYGGPVSNLVDTPDSASRVSITIEGHVEPSLQSRPVADSLVPKEGNSEGDSNELPGATNHQSDNLQGCHGENGEDELNHALKISKEEYEKKEQQEDHLLEEALVASLLDHSKKISEDALSDSELEQFQESTKEHNKTLEMENQDLAHVLEISRNEYLDRELQISLSRALHMSLEDGNVSGTSHDGESDIEEQIISHVLKMSENKNSNQAKNKMDEPLTQQTPKSEEPTHKSPDAGSKEDEDLHGSGSKSEDPDQEP</sequence>
</entry>
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    <accession>E3K7T2</accession>
    <name>E3K7T2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_06137</name>
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    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06137"/>
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    <dbReference type="InParanoid" id="E3K7T2"/>
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    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP80181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="375" mass="42576" checksum="EAEB09B5EF956A4C" modified="2011-01-11" version="1">MKEFGRLGEYNFCWRTGAQTEETPIEQLRSKNTLRSRLDSDLLPSINEQCSRLAGLPRDPPHVGQDPALYLKIIAEIQANLHQTLPQTIQTINALFPNPMPKPDNETNDQGFNEFKIYRLYHLDASIRTDLKDGLKSLFSEFHRILRDFKDGGHTSPCLIEFTGCKINQTIDWAISLSKASELSLIWTLWKNEIPECDEEFEGMLIQMGPTRLLDGEHEALLSPPAVELGTSLIPVFKLSRLFFNKLSQERVTKEDVASFTEMSSAQLSSLDGWIEQIFSSIASMHFSIKNADQDDPVNTSAAIIDELETLKALFRSYQLLIDKYILPNLFPNSLDLSSRVYFKDWLDSWTTSFSKAIHNTILAANTYKSSTISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K844</accession>
    <name>E3K844_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06196</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80240.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324659.2">
        <property type="nucleotide sequence ID" value="XM_003324611.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K844"/>
    <dbReference type="GeneID" id="10534582"/>
    <dbReference type="KEGG" id="pgr:PGTG_06196"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06196"/>
    <dbReference type="HOGENOM" id="CLU_1866132_0_0_1"/>
    <dbReference type="InParanoid" id="E3K844"/>
    <dbReference type="OrthoDB" id="2516998at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP80240.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="137" mass="13535" checksum="EE5C2F577A967076" modified="2012-04-18" version="2">MLFGNLSQLLRLSILIVITISVSQISSFPQYLTSDPYGGGFNNGGIRGNGYYGGTGIGGGGLGGFGGGGLGLNSGFGGGLGGGLGGGFGGGLGGGFGNRGYGYYSDASSIQTPLNSTKLYWKSLLSLAVTGIFLLLC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3K9L5</accession>
    <name>E3K9L5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07363</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP81111.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325530.2">
        <property type="nucleotide sequence ID" value="XM_003325482.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K9L5"/>
    <dbReference type="GeneID" id="10536433"/>
    <dbReference type="KEGG" id="pgr:PGTG_07363"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07363"/>
    <dbReference type="HOGENOM" id="CLU_327352_0_0_1"/>
    <dbReference type="InParanoid" id="E3K9L5"/>
    <dbReference type="OrthoDB" id="2497666at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="363"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="392"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="188"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81111.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="879" mass="96604" checksum="9179E18C6E21E2D5" modified="2012-04-18" version="2">MSYVHCQHTSADTPPCRCPVWEAPGDEEPTIKSCDTCGHRSEWHLPLVESTDNEGDNEMERCKSSWPNGQPCTCPTWIAPEDPSQNPGCALCGHKKGWHRPTITRASSTQGTPSKMQLPSRAEVFKLEESNWHSRAQSCGTGSADNSLKVRTCSANSWAPGTKSSSASNQGISDPPANGFNSFFTPDSSTASPNSAWSSANPLPTVHIPPVEVQLAAEHMRFILSELDDDEDEQETSFSNTLNSPSNHSRGESGNRSLHNHDRSFSRASQSSLYSFEAPMSNQQWTMDGRSPSPSFRPADTQSIRSNSSSFVSVAMPRASVDVNTCSGSMSFSSLVEGVYSRSPSRTQPKDYNIRLSELDLTGGSSNSANSHLRPPPGLPLLAQTTSETYVEELPVDRPRHPSEERTGRFAFNPTDIASNTPLDESDADGSVKKVKPFQSNIQSVILEEIPVLDQHTEIAATLKPISLPEQPIYNPQQSERQRVESYQTKDQAISLSISPSPSSTTYSPGDYLNVTIQLRNSTNNQEIVSPLEFEQQLSKWKKITFQLSGTVTQNGTTEHEIFNLSQNVYLSDQRELNANKTGWRFKLRIPTHTNCKCGPGHLPLPSSCSNSVGHVSYHLILKGKKKAFLSKASTTAESSLFVGLNLKSNISDPRQQLLTFPESVRPLTSWIGLNGAQKAAITNTLLTYQVEFLSSNTLRILYEVELQLSPDSELSNRNFGLFEELSSNLKVTITSLNQLIQSHANTGNGYGNVAQEDYQKNFKTRDVRSKAMPTRSDTTGLITWKISGYLHMELFGPADSASSHEPIQKKCLMGQLKSYAAKADLPSSAKNMTSQWILRASIQSVILSQPLNIRGTLVDLQSTCAARSIVQGIPLTLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>E3K9T1</accession>
    <name>E3K9T1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 14">Phosphatidylethanolamine N-methyltransferase</fullName>
            <shortName evidence="13">PE methyltransferase</shortName>
            <shortName evidence="13 14">PEAMT</shortName>
            <shortName evidence="13">PEMT</shortName>
            <ecNumber evidence="13 14">2.1.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGTG_07196</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 14">Catalyzes the first step of the methylation pathway of phosphatidylcholine biosynthesis, the SAM-dependent methylation of phosphatidylethanolamine (PE) to phosphatidylmonomethylethanolamine (PMME).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine + S-adenosyl-L-methionine = a 1,2-diacyl-sn-glycero-3-phospho-N-methylethanolamine + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11164"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64573"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="EC" id="2.1.1.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="13 14">Phospholipid metabolism; phosphatidylcholine biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="13 14">Endoplasmic reticulum membrane</location>
            <topology evidence="13 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13 14">Belongs to the class VI-like SAM-binding methyltransferase superfamily. CHO2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13 14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.17" evidence="13 14"/>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP80944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325363.1">
        <property type="nucleotide sequence ID" value="XM_003325315.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K9T1"/>
    <dbReference type="FunCoup" id="E3K9T1">
        <property type="interactions" value="60"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K9T1"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="InParanoid" id="E3K9T1"/>
    <dbReference type="OMA" id="RIWYSVG"/>
    <dbReference type="OrthoDB" id="4583at2759"/>
    <dbReference type="UniPathway" id="UPA00753"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:phosphatidylethanolamine N-methyltransferase activity"/>
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        <property type="term" value="P:phosphatidylcholine biosynthetic process"/>
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        <property type="entry name" value="Phopholipid_MeTrfase"/>
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        <property type="entry name" value="Phosphatid-EA_MeTrfase_fun"/>
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        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE N-METHYLTRANSFERASE"/>
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        <property type="entry name" value="SAM_CHO2"/>
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    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="5 13 14" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 13 14" id="KW-0808">Transferase</keyword>
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    <keyword evidence="8 13 14" id="KW-1133">Transmembrane helix</keyword>
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            <begin position="112"/>
            <end position="133"/>
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            <begin position="479"/>
            <end position="498"/>
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    <feature type="transmembrane region" description="Helical" evidence="13 14">
        <location>
            <begin position="560"/>
            <end position="580"/>
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    <feature type="transmembrane region" description="Helical" evidence="13 14">
        <location>
            <begin position="592"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13 14">
        <location>
            <begin position="667"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="353"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1018"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="24"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="356"/>
            <end position="368"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="393"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
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            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022691"/>
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            <dbReference type="ARBA" id="ARBA00022824"/>
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            <dbReference type="ARBA" id="ARBA00022989"/>
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            <dbReference type="ARBA" id="ARBA00023098"/>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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            <dbReference type="ARBA" id="ARBA00023209"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023264"/>
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            <dbReference type="HAMAP-Rule" id="MF_03217"/>
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    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU361122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="EMBL" id="EFP80944.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
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    </evidence>
    <sequence length="1107" mass="127900" checksum="C1B3D346096D1119" modified="2011-01-11" version="1">MQPSSTTSLKPGVVLVAPSPPADENPKKNSFQKDEADKEDDQQQDEQGKSRQKSTYGKTMDGTVFKIPETHNMISSLFDPRLPKSSLDLITLSTLIAQTIPLMIFPRKKLKTFYMLSFLFWRLTYNVGLGWVLKKQSENKFLIKIIKNLNLFNSKFNPRLSNWIEKQLKSKMSTNNLNYDLNQYPIEFNVWLLFRQLVDIILLNDFFSYFCLSYCSLNLPNQQQSQQDPLPNQSNLSLFLIRCTAGVILVGFNIWVKLDAHRIVHDFAWYWGDAFFLSLQELVFDGVFEMAPHPMYSIGYAGFYGASLITGSQSVFFVSLFAHLCQFGFLVYFENPHIQRTYGPQARPLTAASPRHALRSRSHPRKKIQPLNFNSPNENPKAVSPDYRDTQHDTSSVHGSNTFHDPPQNPVDPAEVREELEHRLFRRKEMILIDNFDKFRSIDFVTVLVIGYNFLILFIKEEDPSHIKKIYNNKGLLISSLNAIAWRLWFSFGLGFRLKQQSQNKMMVKHFLTNYYYPIGKFRHSQQKNEDEINDDDQNDLIDELKFEVIEECFQNWKGIYNLSLIMTYVSFAIMSIKFHKLGDQWELSSEVIRITFGLCLVGIHVWTAISTYEVLGKFGWFYGDFFVDDYPMELTYAGIYRYLNNPERSMGGAGFVGMSLMSGNKFVFALALGSVLCHWWFLSFVETPHMKKVYGDRIRKEAGLTKVLKQNAQKLAARRKKLDLRIDRESCSSMGGGKSDEREGKGFERRFKEVQGTFEFIYDETVQALDEFLEKSAPRLSGVVRDTKVLLQSSGERLILTRIANDLSSYDTTQYRLAIQESRYHRPRDPASNRLRFHMGEPIRLQWNSPWNHSRADWVGCYRRGSNKSGLVTEVTSSGRWCGLFKEEWEGDMHIGKRRQEGDAELDDAGKELKLENEAAKPRKTGWVEFKGERLPWEEGVYEFRYHHDGKYNVMAVTEPFEIYVERIEQDVGNMKEIEDKLRNLVGYGLGMEANLIPMILRKETARILKDEFTKPSQNGIHAANGHQPATEGKVGENGLSDEGSQEEEDRFTVMSLDEAKRIQRLIGIGIGIELGIEIILEDCEINRLAKRIVFVKSLLKPFLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAN8</accession>
    <name>E3KAN8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_07066</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81445.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003325816.2"/>
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    <dbReference type="GeneID" id="10537183"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07066"/>
    <dbReference type="HOGENOM" id="CLU_555670_0_0_1"/>
    <dbReference type="InParanoid" id="E3KAN8"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990837">
        <property type="term" value="F:sequence-specific double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017970">
        <property type="entry name" value="Homeobox_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051892">
        <property type="entry name" value="LBX_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24336:SF8">
        <property type="entry name" value="LADYBIRD EARLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24336">
        <property type="entry name" value="TRANSCRIPTION FACTOR LBX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00027">
        <property type="entry name" value="HOMEOBOX_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 4 5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="3 4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="7">
        <location>
            <begin position="405"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="4">
        <location>
            <begin position="407"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="299"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="339"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="471"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="311"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="340"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="480"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP81445.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="491" mass="54278" checksum="8A6681858C3D07F3" modified="2012-04-18" version="2">MSLPEADGMRARANSNGGGCLDHEIISRAYGEDGCEIPLAFNDHNLRAEWSSATNLGAKIPTHTGSSSTSLAPPLGLSSPGFELPANPVTLPQATWDPLIFPSQLEQMNLGNPASDDFLRHQFDFLNTNSQTELLSYDYNQPVMVSSPTCQLSETDSFLPFENSTQTSATYHQMSLPNPNIFSKATESREISPFMPFSQEAASGLAVCSSASQPLPILSPPLGLDYYTLETSRAAPLIGSKRSLPIMNRGEMSQNAHEIKESLLGFEGRGGPGLMHDFNVLPNTTSKYPIESFHETGESSSHLSWDMRTRSSSSRSPSYDQSSSEHVKYPLADANTSDYHQSSHLLSSLHPTEFSPHNLPARSGAYQPEKSTTSGKKQNSPQVSGSRDYQTNQKRVGSLIDIHHLTNHVPRRRLTVEQEQILLRRFAVQEYLSKREMEELAEKLKIPTSQLRTWFGNRRSKLKAAARQKAKMEAGMGHLTDAELRSLNKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KB38</accession>
    <name>E3KB38_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81751.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326170.2">
        <property type="nucleotide sequence ID" value="XM_003326122.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KB38"/>
    <dbReference type="GeneID" id="10533459"/>
    <dbReference type="KEGG" id="pgr:PGTG_08000"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08000"/>
    <dbReference type="HOGENOM" id="CLU_662457_0_0_1"/>
    <dbReference type="InParanoid" id="E3KB38"/>
    <dbReference type="OrthoDB" id="2518090at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173703" evidence="1">
        <location>
            <begin position="26"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81751.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="415" mass="46971" checksum="11FFE0A1F5B86659" modified="2012-04-18" version="2">MASTLQSWLLGLTLILLCLLRLSTTSPPISICADSEVPDINAVDRQTEGGSIPFEDLSVSDFQPNSKRRRITIDFFGDLGDLGDRLSSDSGTQADDVAHVDIPIKSADAGSSGLHREWRGSPVSHSAIDWSKHAIAGTQEGIRPVPGARRKRMEDVKNRMAKILEALEYFHQLAREQGVDKKIFGPQIKNTDAKLNQWFYDILFKKTEDSYPLFGQFEIKLTSVFSPENLFGSLQKEISKVIMGWKRPKLENIYEVALSLLGYWYQTTALKLGRADLKNNPETYWNLMAQLTVDRSPQGGLFSVEYNEGEDFFSTNLKRYIVEAKTRHQWEPLFVFLNSRRERNFGSSVTKDIKNRIEEKLKSSGLSRGLDATSKTKIADVPIYLIPSLQVKDTPRIQKCPEILVKNVNQSWKPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCT5</accession>
    <name>E3KCT5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_07628</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82231.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326650.2">
        <property type="nucleotide sequence ID" value="XM_003326602.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCT5"/>
    <dbReference type="GeneID" id="10529604"/>
    <dbReference type="KEGG" id="pgr:PGTG_07628"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07628"/>
    <dbReference type="HOGENOM" id="CLU_1611585_0_0_1"/>
    <dbReference type="InParanoid" id="E3KCT5"/>
    <dbReference type="OrthoDB" id="2508874at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP82231.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="165" mass="18629" checksum="83F75A63669E418D" modified="2012-04-18" version="2">MTIPTESIPSPPDTFQESQVPIVFQETQGSEYIPNSQELESQDFPESLPVPDGSELYCKFDLDYSIWVNNSGNTSLVNNLRPAKPPCQLRMSVTDMSFSDFKTRVYNHLAATPDHSLLKSEHLVKALTNADKADQLQWCCSIKNCERVVTDDASFKSFVVFELMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3KD06</accession>
    <name>E3KD06_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Mitochondrial import inner membrane translocase subunit TIM22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_07667</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential core component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. In the TIM22 complex, it constitutes the voltage-activated and signal-gated channel. Forms a twin-pore translocase that uses the membrane potential as external driving force in 2 voltage-dependent steps.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the TIM22 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Mitochondrion inner membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the Tim17/Tim22/Tim23 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326689.1">
        <property type="nucleotide sequence ID" value="XM_003326641.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KD06"/>
    <dbReference type="FunCoup" id="E3KD06">
        <property type="interactions" value="353"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KD06"/>
    <dbReference type="GeneID" id="10529643"/>
    <dbReference type="KEGG" id="pgr:PGTG_07667"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07667"/>
    <dbReference type="eggNOG" id="KOG3225">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_091077_1_0_1"/>
    <dbReference type="InParanoid" id="E3KD06"/>
    <dbReference type="OMA" id="EERWIQW"/>
    <dbReference type="OrthoDB" id="75343at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042721">
        <property type="term" value="C:TIM22 mitochondrial import inner membrane insertion complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030943">
        <property type="term" value="F:mitochondrion targeting sequence binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045039">
        <property type="term" value="P:protein insertion into mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039175">
        <property type="entry name" value="TIM22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14110:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02466">
        <property type="entry name" value="Tim17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 9" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP82270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="179" mass="19375" checksum="EE92738E9AA6754A" modified="2011-01-11" version="1">MASGFLPPYIIPGQEPGTTAEERQEIYKYLHWTRLGQAGQESCVAKTILSGAMGLGLGAMFSLMSSSFAYEDPLRRDPNFDTMKFDKKARVMFKEMGRGMWSTGKGFGKVGALYAGLECCVESYRAKSDLWNSLVGGGLAGGMLAWQSGMKAMLFGAAGFAAFSGAIDAYMRKEPPDDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3KEX8</accession>
    <name>E3KEX8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NodB homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_09991</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178284">
        <property type="protein sequence ID" value="EFP83023.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327442.2">
        <property type="nucleotide sequence ID" value="XM_003327394.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KEX8"/>
    <dbReference type="GeneID" id="10544735"/>
    <dbReference type="KEGG" id="pgr:PGTG_09991"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09991"/>
    <dbReference type="eggNOG" id="ENOG502S2CW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021264_11_2_1"/>
    <dbReference type="InParanoid" id="E3KEX8"/>
    <dbReference type="OrthoDB" id="2125469at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10951">
        <property type="entry name" value="CE4_ClCDA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46471">
        <property type="entry name" value="CHITIN DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46471:SF2">
        <property type="entry name" value="CHITIN DEACETYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="NodB homology" evidence="9">
        <location>
            <begin position="95"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP83023.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="323" mass="36344" checksum="DD2E1BE483A18DCD" modified="2012-04-18" version="2">MTLSTGLKLNRWRMFSSGLILLAGLINWSMAQRRHSHSFVLRNTHENSNDLSEKNEVRLRSIGTNSNHNMSLHQRDLIARGDSAVPVFNTCSTPSSFSLTFDDGPSQFSSKLDATLAEANFKASYFINGNNVGCIYDFADLLIERFRKGHLIASHTWSHVHLNQGSYQQISQQLELLENAMIKILGVKPLYFRPPFGEYNDIVLQVLQDRGYKGLILWSQDSQDSLASPPSSSEIIANYRSYPDQTNVLNHETTSLMVDEVIPGVVPILKEKGFNLLTVWVRIQVIGTCPFNNLDRGMNHGLARALQHLKAMSELKATSFNSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGB8</accession>
    <name>E3KGB8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_08529</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83343.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327762.2">
        <property type="nucleotide sequence ID" value="XM_003327714.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KGB8"/>
    <dbReference type="GeneID" id="10545059"/>
    <dbReference type="KEGG" id="pgr:PGTG_08529"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08529"/>
    <dbReference type="HOGENOM" id="CLU_2414361_0_0_1"/>
    <dbReference type="InParanoid" id="E3KGB8"/>
    <dbReference type="OrthoDB" id="10307789at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173865" evidence="2">
        <location>
            <begin position="24"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP83343.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="95" mass="10086" checksum="E0618FDDEF82681C" modified="2012-04-18" version="2">MFGKNLLTLFLIFGILEVFPTLALPNEGVTRPLLIKEESSTAKAAGQKAQSAGEADSNKAQRAAKSGEKESKDKSTGGSDQGKTSYFGQGHVHMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGD1</accession>
    <name>E3KGD1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_08542</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83356.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327775.2">
        <property type="nucleotide sequence ID" value="XM_003327727.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KGD1"/>
    <dbReference type="GeneID" id="10545072"/>
    <dbReference type="KEGG" id="pgr:PGTG_08542"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08542"/>
    <dbReference type="HOGENOM" id="CLU_032241_1_0_1"/>
    <dbReference type="InParanoid" id="E3KGD1"/>
    <dbReference type="OrthoDB" id="2507256at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="233"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="362"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="46"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="265"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="312"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP83356.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="426" mass="46464" checksum="F03113DA2522CF27" modified="2012-04-18" version="2">MAAFRSEQAQMKKLKALEKAKEKQSKGRVRASRRSTQTSASDQTQGNSTQQSAPVNSQPLVPDTNPPFNTYDYENVCSYLEEEANYTRLYGDGSKTTVGKTKVTKGAAYDMKQFMKAKDFAENTRARIEEGDGLPTLAELLEKKWPCYERMYAIFGGKANMTPLAQFDSGVGADLYAESTNARDVDNPSLSPEVFFSGWEESEVDQPPSGANTPAAGLDLIRCQTAFDRLTSLNIPNSEGEGDLDDDLLPPPLDFSGTAMDPSPSLMTQRSISRATQGSAIPSANGGVLLPMSSQLSTPDPARLSPVLGGSATRQAFPNQRSTEASPAGPNREGNGKPKATLASAFESSNLEKFAYLKAHMAWEKEKENKRLEWEKERYNKEVERATKGAQGQAKLAEAKLNTAQEWISQGKLSTEVDLLLKAIYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGJ0</accession>
    <name>E3KGJ0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="7">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_08601</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="7"/>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83415.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327834.2">
        <property type="nucleotide sequence ID" value="XM_003327786.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KGJ0"/>
    <dbReference type="STRING" id="418459.E3KGJ0"/>
    <dbReference type="GeneID" id="10545132"/>
    <dbReference type="KEGG" id="pgr:PGTG_08601"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08601"/>
    <dbReference type="eggNOG" id="KOG0351">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3176">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011478_1_1_1"/>
    <dbReference type="InParanoid" id="E3KGJ0"/>
    <dbReference type="OrthoDB" id="2505285at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="77"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="414"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="641"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="791"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="415"/>
            <end position="424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP83415.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="813" mass="90760" checksum="E3E01F6413FDC864" modified="2012-04-18" version="2">MAVSKTNRTQSPRKSGRKSPPKTDPKIKKTRQTSGGSVTVTKKLLRANDDSLKIEIATKAAEFYDGQEAKTLQIQAVANLVRGRNTFLLAGTGYGKSRIPEIYHKLLPQERKPVVIVLNPLDALGDNQVEEKKGKFSAINLTKLTFNKTVANKIQKGDFNFVYLSPEIFMNNKMWDSVYFSSEFQERLALVVVDEAHMIYLWGLVERDGRRTASIFVRLEDIGIFRPYYGQLATHLQCRNNSPILMLSATCRPVAVDAILKNLKLTKELIDIIEGELTRTEIRILRINMTESLQSNLDLKNIFPLKEKTSDQDIVPTLIYSGTRARTLTVLEVLDMARGTPGGCLNPDNTLARRFHSCTGDKDKVSCIEGYSQGSFPIISATMALGLGQNWKRVRCVIHMGRADPANVSQMIGRCGRDGGKNSEEDFSNPTDQSDDDRMDALAITSCCLRVAFAMDNKLGYIPLSKDDPDYVKEVEHERTAGFPPCKCSNCAVVSGQQLVENLRYLTKENFESAMDNTLDFPPPDSNDAVLKKNRTRRAANAALGTENDQVILARFKADMITSFHQFYEAQMGCSARFSASSLFHDEHADTLLENLDEIQSAADLYHLIGGEVICGQLEFLYDLIGRFKENDLYQEHLDNQERLREENEEKKKTRKKEAAARYRANRRMRLLAAKTHVCSQSENMPSPPSSTIDRDLEITVDKKGIRTTRNHSENMVVLQATDTVLRSSESMVNKEETRKQRKREADARYRAKKKAQRLGGPVKESESINQAGAAVVDSTVEANVSNPMAVSDAGANVSNPMAVSDAGLDAGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGS6</accession>
    <name>E3KGS6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08687</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83501.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327920.2">
        <property type="nucleotide sequence ID" value="XM_003327872.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KGS6"/>
    <dbReference type="GeneID" id="10532179"/>
    <dbReference type="KEGG" id="pgr:PGTG_08687"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08687"/>
    <dbReference type="eggNOG" id="ENOG502SA5Y">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1366847_0_0_1"/>
    <dbReference type="InParanoid" id="E3KGS6"/>
    <dbReference type="OrthoDB" id="2498675at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38849">
        <property type="entry name" value="SMALL SECRETED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38849:SF1">
        <property type="entry name" value="SMALL SECRETED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173377" evidence="1">
        <location>
            <begin position="22"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83501.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="200" mass="21849" checksum="60418A71E8F38F54" modified="2012-04-18" version="2">MKSPFKSFLVAGLCFSQLLDCSPLSHLMSRDVNIHKLIKKSIIKRLEYSDFQISDGLSGNCSKKAERVFLTPYNLTQVTVLNTGSPTPIGPVNGVAIDKTDLDVCKKMSRAAIDAEDSFTTAIDESGGPKTELGKQFQNGKICNKVLKLTASVLCLQMEIQLGAANSSRRKKLAEQITKLEETILIDQAASGQKQRSVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHP5</accession>
    <name>E3KHP5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA-dependent RNA polymerase</fullName>
            <ecNumber evidence="1">2.7.7.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_09533</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the RdRP family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.48" evidence="1"/>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83820.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328239.2">
        <property type="nucleotide sequence ID" value="XM_003328191.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHP5"/>
    <dbReference type="STRING" id="418459.E3KHP5"/>
    <dbReference type="GeneID" id="10532507"/>
    <dbReference type="KEGG" id="pgr:PGTG_09533"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09533"/>
    <dbReference type="eggNOG" id="KOG0988">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001366_2_1_1"/>
    <dbReference type="InParanoid" id="E3KHP5"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031380">
        <property type="term" value="C:nuclear RNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007855">
        <property type="entry name" value="RDRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057596">
        <property type="entry name" value="RDRP_core"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23079">
        <property type="entry name" value="RNA-DEPENDENT RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23079:SF55">
        <property type="entry name" value="RNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05183">
        <property type="entry name" value="RdRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0696">RNA-directed RNA polymerase</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RDRP core" evidence="3">
        <location>
            <begin position="439"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1173"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1177"/>
            <end position="1186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1187"/>
            <end position="1197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU363098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP83820.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1246" mass="140665" checksum="305854A6A8AAEA27" modified="2012-04-18" version="2">MEVYARDLPTAGTQLETKLALITAVSQVIHRPPVLKSNEIRSNFQVDVRPLTHPQAHSHLQPSFHPTHTNRSQRLIATLTFPTSELGHRFLSAVVRNPVKIFNNLSPIQFEPSLRNGRRIIPHPQLIKSLKDSRFKETTEVIQELKELELTQKPIPISLIDFGRTCQHLNKPAFSSEYTLCLPSESSQLTFDIDQKFILLTWTTQERSKKIVIQLQTLRKIEACSPWVILELARPPQFEELVNKIPSYFTGPLPPLDKHDAGPRIRVHGYPHTKADVFPFVNNQIRIQFASETAFQNFCSLKVAVPLPKIISFSRTIVEKDHYDPERIKAIHENLVCFTIPVAFQIESLLHNSILLPYQILRVCQALMGIDECVAERALVQFIEKYSENNQGTKNGLQASKPVYLQVLQEALKASAQEANLNHLKIEGGDSFPCRTVTITPTRLSLQGPQIEQSNSILRLYQQTENFLRVSLADETGHRLRASREVEIHHLLRNRYRPFLTKGMILCGRKFEFLGYSNSALKDHQAWFVCPFYKGDQLVTAASIRAKLGDFSKVIRIPARYMARIAQAFTSTRRSLTLRPSEVTRMDDVERNGSCFTDGVGTISKDLVDEVHRVLNVGNIRKSISSTCYQIRLGGFKGMLSLDPTLKGKVVRMRPSMDKFDAPDSLTLDIAGTFTKPLVAYLNRPLIKLLEDLGIPSEVFLRLQDKIIRKVENSRSSPKLAGNLMQQYSMGSGSGMPSMLHKLSELLVDDRAVESDFIEECYDLMTVQCLRDLKYRGRIPLDKSYTLVGVADEDNFLPPDSIYVCVQTPNSAPMYLEGSFAVTRSPSLHPGDVRVVNAVGKLDPKRAPRLSSLVNCVAFPIQGERSLPSCLGGGDLDGDLFTVIGLPQLVPKRSKIQRAASYSPPEMRKLDRDCTIADGADFFLDYISSDLVGMIATRHLHIADQCPEGTLNESCIKLAELHSDAVDYPKTGVPVNIRSLPNPPVRMKPDFMCHEHDSSKEGEDYYESPNVLGKLFREIPADKIDIFIPASKIDDDIAELHRIRRTERKKFGGVYEPLDSNDRLSKLIVRRIKLKYPSSEEHRSILRFDDRLREELRSLMGWFGAELMKICKLNSVSTGQAGHHLAESEAFLGVIIMPCDRFQKRTALSRLLEQTSELFGFLKSEIIGSSARKTDEEPAEIDELDDNDYRSGDSSERTADRNDRIFALHRAFVAWTVATHSQSNLFGTHSFGFISLGILLNQLFLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIA3</accession>
    <name>E3KIA3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09741</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EFP84028.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328447.2">
        <property type="nucleotide sequence ID" value="XM_003328399.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIA3"/>
    <dbReference type="GeneID" id="10532723"/>
    <dbReference type="KEGG" id="pgr:PGTG_09741"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09741"/>
    <dbReference type="HOGENOM" id="CLU_2122268_0_0_1"/>
    <dbReference type="InParanoid" id="E3KIA3"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84028.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="114" mass="12104" checksum="0553A44521BCFCF6" modified="2012-04-18" version="2">MYSRGSVNHKGATQSFDQGLNSSSPLSAINPSSNQNTENVAQKAAQILFLLSQGNPDPIAHQSSATRLSRSNYPDIKALSQACYSSAAQISNMVGTLRLLLVATLGGYVFSNEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIK0</accession>
    <name>E3KIK0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PIG-P domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_10503</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328544.1">
        <property type="nucleotide sequence ID" value="XM_003328496.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIK0"/>
    <dbReference type="STRING" id="418459.E3KIK0"/>
    <dbReference type="GeneID" id="10545303"/>
    <dbReference type="KEGG" id="pgr:PGTG_10503"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10503"/>
    <dbReference type="eggNOG" id="KOG2257">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118214_0_0_1"/>
    <dbReference type="InParanoid" id="E3KIK0"/>
    <dbReference type="OMA" id="TRWPAIE"/>
    <dbReference type="OrthoDB" id="690928at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052263">
        <property type="entry name" value="GPI_Anchor_Biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013717">
        <property type="entry name" value="PIG-P"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46346">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46346:SF1">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL N-ACETYLGLUCOSAMINYLTRANSFERASE SUBUNIT P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08510">
        <property type="entry name" value="PIG-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="102"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="domain" description="PIG-P" evidence="7">
        <location>
            <begin position="65"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="155"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="158"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF08510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP84125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="200" mass="21843" checksum="E3E3AC6667959E9B" modified="2011-01-11" version="1">MPAKEEEARIKESERDSSSRNKGASPTTQDSRSTELEPEERGGGTGRRKRQMKLPAPGTNALATSAFATYVLSTIIFTIYLLHGLLPSKTVEEVLGLSYFPAQVWTTILPGWLTIAIWYLFLAYFALNLSRTPALDGPDGLAAITDRAALALLTPPSTRTPPPPRSSPHPTPHPSADSSDLPVPILRDLPCQVVNQFYFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJ17</accession>
    <name>E3KJ17_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small-subunit processome Utp21 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_10670</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84292.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328711.2">
        <property type="nucleotide sequence ID" value="XM_003328663.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJ17"/>
    <dbReference type="FunCoup" id="E3KJ17">
        <property type="interactions" value="837"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KJ17"/>
    <dbReference type="GeneID" id="10545636"/>
    <dbReference type="KEGG" id="pgr:PGTG_10670"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10670"/>
    <dbReference type="eggNOG" id="KOG1539">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002774_2_0_1"/>
    <dbReference type="InParanoid" id="E3KJ17"/>
    <dbReference type="OrthoDB" id="10250769at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034388">
        <property type="term" value="C:Pwp2p-containing subcomplex of 90S preribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059157">
        <property type="entry name" value="WDR36-Utp21_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007319">
        <property type="entry name" value="WDR36/Utp21_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22840">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22840:SF12">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25171">
        <property type="entry name" value="Beta-prop_WDR36-Utp21_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25168">
        <property type="entry name" value="Beta-prop_WDR36-Utp21_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04192">
        <property type="entry name" value="Utp21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="WDR36/Utp21 N-terminal" evidence="4">
        <location>
            <begin position="46"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="294"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="1">
        <location>
            <begin position="338"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="domain" description="WDR36/Utp21 C-terminal" evidence="3">
        <location>
            <begin position="778"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="832"/>
            <end position="854"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="833"/>
            <end position="847"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP84292.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1004" mass="109779" checksum="FBC1DB9B41837430" modified="2012-04-18" version="2">MSTSLYAPFRSLGHVTTDVPFVIHSHSTKDSDVLGSTKAQTNSQVILTSVGLGWALWSADSLRLLYVGPLTPTPITWLNVHTSGLVLASAGSQIYIYKRTRVIGTLKTSSQKTQSGDSSSDNSDNEADLGPECWIDQFKKVSTQNITIQEFISFGNQVVGLSSCGKTLWVWNLDTKELHITIELPSTFGLATTLVHPSTYLNRIVVASQEGQLAIYNIQTGALIHIFQPALFTTPSKARCSSDKIPITRMVQAPAVDIIAVGFADGWCSLVDVKFGEEILAVKMGQSDGSNLSTNAVLDSITGITFRSDTQVMVTSSSQGHLAIWNLNEGGRLLHIIREAHDSGISNVYFLPGQPVVVTASGDNSIKLWLFETPTGLPRLLTHRSGHQRPPHLIRYYGSNGKTILSAGQDKTLRAISIVRDSRSFELSQGSITKQASQLAVPVSSLRLLPITNMSHSTSRSKDWDDLLTCHEGSASGRTWSVQNKRIGKHSFSLIEKKSFQLDPQGEIAKCVCVSACGNYGLIGTQGSGKVGMWNMQSGMKRREFILPGSSGKGKQNDMILNVVGVATDALNQLVMVATRSGHVHFYDFISGSLRQTVTLPSGATSILLQRDTGMLAINCRDNQIRILDTDTFEVIRQLQGFTKPILDMTITADSRWLVATSADSVIRTFDLPTGAMIDAFRTTSVATSVTFSPSGDFLASAHVDSVGIFLWVNRAQFSGFSYKSLPENYEIPLLETPVLDGVEDKPTEELEAMLRPDAWGSDPCADSHNDHITSKPLEEGLITLSCMPKSKWQTLLNLEIIKARNKPTEPPKAPERAPFFLPTVAGTEPRFNVTSKGLENPNSSAPADQKKKRKLDLLDSTKVEVEFTKRLTQSEASGEYEEFFEYLGNLSPSALDLEIRSLSDIQHLLSLIKALETRLKEGQDFEMVQAIMRVFLKVHGPRLAGSEGTSIFGLPDQDDERHKILPAINHLLAVQEEVCAQLNRLVDYGIGVSAFVRGLTGVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>E3KK82</accession>
    <name>E3KK82_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="7">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_10866</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="7"/>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84707.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329126.2">
        <property type="nucleotide sequence ID" value="XM_003329078.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KK82"/>
    <dbReference type="STRING" id="418459.E3KK82"/>
    <dbReference type="GeneID" id="10526674"/>
    <dbReference type="KEGG" id="pgr:PGTG_10866"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10866"/>
    <dbReference type="HOGENOM" id="CLU_011478_1_1_1"/>
    <dbReference type="InParanoid" id="E3KK82"/>
    <dbReference type="OrthoDB" id="2504946at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="42"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="267"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="619"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="628"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="659"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP84707.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="708" mass="79535" checksum="C2D5B9278564BDE1" modified="2012-04-18" version="2">MEGIHAPAKVQASTDEEVREKIAIQSREKYDQEPKPLQVETVLNLLRGRNTFLLAATGFGKSRISEMYLRMLPKNPHGDTIGVVVVLNPLDALGDNQVEEKVAAGFTAINLTMPNFSEEVCLDIQEGLYDFVYLSPEMFLDNEAFSNVYFSPKFQSKLALIVVDEAHMIYSWGLVEGGQKKLKTIVRHQDFSAFRPHYGSLATQLLTKNQAPLLLMSATCRPAAITAIKKNLKLLDTHIDLLRDELTRPEIRILRIPMKSSIQSCEDILQVYDPKSHIPDEQLVPSLIYSGTRVRTLQVLEVLDQARGTPGNHLNPRNSFARRYHACTGELDKKDVIDDFASGKVPVLSCTLALGMGQNWKLVRQIIHIGRGDPSLICQMIGRCGRDGRPGLAILMVETNRPNGKNSIADFPPNQVQTDEDRMDALAITPVCLRIAFSMDNLIGYIPLDFNDPLYQAEKLREELNGFAPCMCSNCMEHAGKVLVQNLPNLNKDNFTDYTLNRVALNAVATTETKRKYTINQNGPTTKKQERVRLEPLKKILQETFNNFYLNMYGESGSLVPSDLFGDLEVEAIINYFGQIQSKHDLRQVIKGEMVKGQLEVLMKSIEGYRVRGAIETNALETGAPDKPSTSSQTNLNSNPDNFTPAKRPRKNAVSPETLRQREQAAAERNKAKELKLELQKKEQEAKARRSAWHLQNMNEIKKAHGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KKB7</accession>
    <name>E3KKB7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_10901</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329161.1">
        <property type="nucleotide sequence ID" value="XM_003329113.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KKB7"/>
    <dbReference type="STRING" id="418459.E3KKB7"/>
    <dbReference type="GeneID" id="10526710"/>
    <dbReference type="KEGG" id="pgr:PGTG_10901"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10901"/>
    <dbReference type="HOGENOM" id="CLU_033090_3_0_1"/>
    <dbReference type="InParanoid" id="E3KKB7"/>
    <dbReference type="OrthoDB" id="10275107at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP84742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="412" mass="47207" checksum="C5EB884872A06AB7" modified="2011-01-11" version="1">MLGSTSQHTGHVALSGVHLRQQADVVIKRFTNLVDKCQSARENDFTLSANDFTLSAQNPTLSIDETELKKGLLLQLESCFLPQLRQQLITLLQLTNPYELLEKAASKFKLILEIQAGLDITLDQILCAIDLLCPQPMQLNIVRINDQHLKELKHYRLHGLCHHIKELVLPEIQEVCFDSTELIRRLNFSTKTYEAKDDVALAARENLMAEVSNCSYLVEHGINWMVATELDTTQGIWRKHTFRIDEHLKYLLMLDPTINIPHKRPRGYVIKLVKSIVPVVKLSRLFLSKSSERGMNRKPLVSLFTTMSSNQLDFISDLPSKTDRQLAEIETLLKEFNGSARERSELKKAVQEITTRFETAFLLILVHFVPLIPDTDVYHNQKYFKDWFATWHTQFTIAIGNLLNEIGIFGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLA4</accession>
    <name>E3KLA4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11248</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP85079.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329498.2">
        <property type="nucleotide sequence ID" value="XM_003329450.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLA4"/>
    <dbReference type="STRING" id="418459.E3KLA4"/>
    <dbReference type="GeneID" id="10527056"/>
    <dbReference type="KEGG" id="pgr:PGTG_11248"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11248"/>
    <dbReference type="HOGENOM" id="CLU_046891_1_1_1"/>
    <dbReference type="InParanoid" id="E3KLA4"/>
    <dbReference type="OrthoDB" id="2507040at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="294"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85079.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="378" mass="40783" checksum="96570AB4A365C4C5" modified="2012-04-18" version="2">MSNPTTQTNDDADNLNLKVSGIVQLKPQGADSNYLDWSFVVLLHLKSLKLAYVLEPKASPTSTEAKLTTPLPSSRVNNSIAVSSFIARTIHPSNLRFIRAHGADAAAMWASLSQAHQDFSSGGRMHWLRQLVVARLTCDNIDSHIESMAVCAERLGSLVTPEKPLTVDDIHATALLTSLMDDWLLCVSLLMNEDSVTSSRIVSALKAESLRRKARREDDTTVSVSKARADYAKGKPCFNEALHCSFCNRSGHDLAICKNAARVLPEHKSGHSSGNTGGHCHPDSARDNRRHAPNNRSSNRAASTSKPPVTAGQATMVELGYESYDEESDFSVPERAGNAAVGTYPVESPSNVDANIDSGCSISMTPHESTVDLTSALC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLG6</accession>
    <name>E3KLG6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP85141.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329560.2">
        <property type="nucleotide sequence ID" value="XM_003329512.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLG6"/>
    <dbReference type="GeneID" id="10527122"/>
    <dbReference type="KEGG" id="pgr:PGTG_11310"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11310"/>
    <dbReference type="HOGENOM" id="CLU_011714_0_0_1"/>
    <dbReference type="InParanoid" id="E3KLG6"/>
    <dbReference type="OrthoDB" id="2499245at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="224"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85141.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="687" mass="74863" checksum="BF09D0617CB71BE6" modified="2012-04-18" version="2">MSTLERLDLYEKVQALAAHPALKPNEQGHIQALLNDIKADYENSDDEDNTGGPEVTCNPSAQSPAGPTGSESYLAPEPQAAKDNQTPNQSPSRAPRIGEFFILPNPIGAPRTTCPCFSQFSSGNTTSPVPADVNMDAHMQDGQHSENSRSTLNTLGQTTFLTRAGNEFLESIEQNHAVLNKRVLVTPASNTASTTSLTTAVPSNSSTSAPWMDDDLAAKRARLERRGSHEDADGSTDDEFDHTDKQPSQGQVKVVLDNGLGRASDFRHGPNGPPPQASRPSEFLTDSMFRDYVIPRRPHEGTGANSNDTRPRADQSQNSSGGPTPSRSARSPSVELFVPGQHLPALEVTRWNTELSTGIPWSIESAEKSIRNISFILNNLPQLISSHPTLPEEATRSLWIDLDHTHQLCVSEGILETAGDLSLITPDMVNMDQVTQPRSARAGSHILAPNLATFFAPPKIAYQRLWAALGQAWGIRRLANVKNEWVQQANDDNNGLYTAYYHFCQSYDNLGASLNDPAGDGASRSAMELVQNFCGAMAGLAFICTKNNMKAKQAKLDKLRTVHHVQHVQMLANLLIFGPTATFTSLHKGPHMTLSNANALLELGSSVLKQKIALGITTPMPHSTLDNLRIHFLNFLIKMGFNQLDNDGVPQVNWKDVHASFYNDFSPSVIAGIFSQDIEIALAHRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLR1</accession>
    <name>E3KLR1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="16">ATP-binding cassette, subfamily D (ALD), member 2</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGTG_11429</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ABC transporter superfamily. ABCD family. Peroxisomal fatty acyl CoA transporter (TC 3.A.1.203) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178294">
        <property type="protein sequence ID" value="EFP85260.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329679.2">
        <property type="nucleotide sequence ID" value="XM_003329631.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLR1"/>
    <dbReference type="STRING" id="418459.E3KLR1"/>
    <dbReference type="GeneID" id="10527249"/>
    <dbReference type="KEGG" id="pgr:PGTG_11429"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11429"/>
    <dbReference type="eggNOG" id="KOG0064">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007587_5_0_1"/>
    <dbReference type="InParanoid" id="E3KLR1"/>
    <dbReference type="OrthoDB" id="422637at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005324">
        <property type="term" value="F:long-chain fatty acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015910">
        <property type="term" value="P:long-chain fatty acid import into peroxisome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042760">
        <property type="term" value="P:very long-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03223">
        <property type="entry name" value="ABCD_peroxisomal_ALDP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000800">
        <property type="entry name" value="ATP-binding cassette sub-family D member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050835">
        <property type="entry name" value="ABC_transporter_sub-D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384">
        <property type="entry name" value="ATP-BINDING CASSETTE, SUB-FAMILY D MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11384:SF69">
        <property type="entry name" value="PEROXISOMAL LONG-CHAIN FATTY ACID IMPORT PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06472">
        <property type="entry name" value="ABC_membrane_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="15">
        <location>
            <begin position="490"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="53"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="62"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EFP85260.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="745" mass="83914" checksum="94E4D65AB5CC7B92" modified="2012-04-18" version="2">MASSRNSPIIQQATMGSKILDGYMRNRPFFQKLFMSSFAVYIVLATTSGLRPKKRNQKLAPPSSSQDPKQQVSSNDPKAIGSVGRSANRKRAAARVEVDAKFFARLNRLLAIVIPSWKSKTASLLVIHSAFLIFRTVLSLYVASLDGQIVSALVRGEGIQFLSRLALWMSISIPATYTNSMLSYLQCKLSIAYRSRLTNRIHDMYLSDTTFYGINNLDDRIANVDQLITVDVAKFSDSLAEIYSNLAKPVLDVILYNWQLSKNVGLEGLIGLTIIVQASAALLRAVTPSFGRYASEEQKLEGEFRFTHSRLIENSEEISFYSSHRSYEIEKNIIERSYFSLIKHINWVYKVRLWHGMVEDGIIKWVWGSLGLMICSIPVFFNINPPPVGVVGEAIKKALPDSDMGGRTQGFVTNRRLLLSASDAFGRVMYSYKDVSELAGYTARVSELFDTMESIKRGQYEKRKVGSANTDDSQKLLQNRGQVVESEDDIIFDQVPIISPNGDVLVKSLSFHVKPGQHLLIVGPNGCGKSSLFRILGGLWPVYGGVVTKPASSEFTYIPQRPYLSLGTLRDQLIYPDSKEQMARRGVSDEDLLRILAIVDLDSIVQREGGWDVVREWRDALSGGDKQRLAICRLYYHCPKYAILDECTSAVTLDVEKIMYEHATSLGITLLTVSHRPSLWKYHKYILQYDGEGGYCFTQLDAEKRLALQEEKQALEQKLLEIPKLVARLDQLNETRLKDQDLHHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3KMG1</accession>
    <name>E3KMG1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Mediator complex subunit 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_11842</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 5 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178295">
        <property type="protein sequence ID" value="EFP85486.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329905.2">
        <property type="nucleotide sequence ID" value="XM_003329857.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KMG1"/>
    <dbReference type="STRING" id="418459.E3KMG1"/>
    <dbReference type="GeneID" id="10544038"/>
    <dbReference type="KEGG" id="pgr:PGTG_11842"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11842"/>
    <dbReference type="HOGENOM" id="CLU_698570_0_0_1"/>
    <dbReference type="InParanoid" id="E3KMG1"/>
    <dbReference type="OrthoDB" id="2505968at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014801">
        <property type="entry name" value="Mediator_Med5_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35784">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35784:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="336"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="385"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP85486.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="395" mass="43262" checksum="9F97530686AD72ED" modified="2012-04-18" version="2">MYPQFNLIIKIPISILIPSGLTRSSPIDRTTFITHFFRRSIGFKTPTPTSDPHALKPRPEHNLYPRIYGQTDQTTRSLYDLSDSAFANLCSIKNFIKHTHHPYHPHQSLCSGLAVLFVSVDVQSKLPSWSDHRIYDPSPLEPIEIIPNKQLTSDPPEHTSRDGLSYLEHKLLVGGCAVGVVGWLLNELTRVGWISASSYPTALLEILQSILLSDAITPTALHLVSARSLAVIRAFDASFSRAPPDHLGPGSDLNRRVQLDLASEMIEPAGFLAYDFGSATGWPDALEAAVRSAVCAETDSPDPTIALWSLLPHILKAVSVGEFVQMTIRAAISASKIDDEHDSEEAGTAQDSRPSLPKLWSKGNNFSSHEFQPADQEAAGDFLKTTATTQNPLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3KMK8</accession>
    <name>E3KMK8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peroxisomal membrane protein PEX16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_11889</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Peroxisome membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the peroxin-16 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178295">
        <property type="protein sequence ID" value="EFP85533.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329952.2">
        <property type="nucleotide sequence ID" value="XM_003329904.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KMK8"/>
    <dbReference type="STRING" id="418459.E3KMK8"/>
    <dbReference type="GeneID" id="10544093"/>
    <dbReference type="KEGG" id="pgr:PGTG_11889"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11889"/>
    <dbReference type="eggNOG" id="KOG4546">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036533_2_0_1"/>
    <dbReference type="InParanoid" id="E3KMK8"/>
    <dbReference type="OrthoDB" id="2021143at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007031">
        <property type="term" value="P:peroxisome organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013919">
        <property type="entry name" value="Pex16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13299">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13299:SF0">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN PEX16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08610">
        <property type="entry name" value="Pex16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="2" id="KW-0962">Peroxisome biogenesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU365003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP85533.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="457" mass="52019" checksum="E33A256D58BBC635" modified="2012-04-18" version="2">MTASAARFIDHFSNREDNYRASSLDCFPTQQSTVNRQPPPPSEHFNCCNILNFHREDFLLSNATRITSIESTLRSLTWFLPGRFKDAELASESLYTALNLLSLYHDRIITKVVTSLPSSQKPAQSSHARYTSAWCAVSSTYKFLAHALAIISHSQLLIEMVARRRLGSKRRWRVVLMLELIKALLRLKLVDLTSRMLVNPPLPERRIDPATLEAQSRNTTTTTAAANDLWSHQASSSSLTRHELNSSNSSSSESTWVGKRTGIVRPLIANLRSPPNDPLIGSGKSLVNDYLSRKALTLEEVLKPLDLVRKSKSNKDKLAEIIWILRPVIYVLTINRFGHRHAVPFLCSLSLEYLSYSLRQSGLQTPQARQLTPPIFFASPRRAAFQSLVSELERNELEARRLAFWSYFLRGPLWVLWTKPKLLQISNKFAHVPIFNLISTAILDYTPLLDEYHYYVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3KN63</accession>
    <name>E3KN63_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11028</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EFP85699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330118.1">
        <property type="nucleotide sequence ID" value="XM_003330070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KN63"/>
    <dbReference type="STRING" id="418459.E3KN63"/>
    <dbReference type="GeneID" id="10544265"/>
    <dbReference type="KEGG" id="pgr:PGTG_11028"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11028"/>
    <dbReference type="HOGENOM" id="CLU_774185_0_0_1"/>
    <dbReference type="InParanoid" id="E3KN63"/>
    <dbReference type="OMA" id="PHRNNQQ"/>
    <dbReference type="OrthoDB" id="2507743at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="9"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="227"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="334"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="126"/>
            <end position="138"/>
        </location>
    </feature>
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        </location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
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    </feature>
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        </location>
    </feature>
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            <end position="264"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="360" mass="39758" checksum="34D6C3B86C6F490D" modified="2011-01-11" version="1">MFGIINLHQSEQETGEAAAHHQNNSNTRKRNRGISSESESEPTHHHQQTTDTTLDHQQPSKKRRKGVAGAILSTALDAALFTSAIGYAAYQFWCGKPLEQEQEENSFVQHPSPKLSIFTKSSSKIPTTTATTNTPATNLADHLEPPPPPYEPRQAEPPPEQLSAPLLRRPRLRSTTSRLHSARSTGPVTHPSSINFVLRPLHDPSLAQLPQSPPPPPPPPPRRSLPQNDELMADFVCSLDPYIPPLTPHHDEQEEEEDEDDDDPEMKAFKAQIQGLIRQGQSALASRPPTLSNDDLHHHPTTTPSPFAHTPALLNPPHLRNILPPSSKISESTTADSNLRLLHLALDKAAAKPASNWWEQ</sequence>
</entry>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
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            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
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    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178297">
        <property type="protein sequence ID" value="EFP85816.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003330187.2"/>
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    <dbReference type="GeneID" id="10544393"/>
    <dbReference type="KEGG" id="pgr:PGTG_11572"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11572"/>
    <dbReference type="HOGENOM" id="CLU_044507_0_0_1"/>
    <dbReference type="InParanoid" id="E3KNE1"/>
    <dbReference type="OrthoDB" id="2509616at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
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        <location>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85816.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="483" mass="53881" checksum="75EE834EF4B535D1" modified="2012-04-18" version="2">MASDMETSDDEERTSRTGAASTSQNQPGSAANADDAEFDRMLGGDTDDDENNDDSDSGIEDPTAALKNQVDRERRKRAHESSQATTRKVRKVVVGQKLPSQSSGLSRSIRNFIKFMMGMPASVSSYPAGPTSTEWGIWHNWTKNRYDKVTAHLEIFSEKHKEVPKPTFKKMYREELDRIRKKLTPPPFIATPDVLNSEINIPMHVKKSCEREIQIAGFGRLTFEWKARSFTTSPWNGTLGTILMKHYYQWAKSQPGTFWDETSSMEKILDRWVQGQGKLMKKADRSDGKSLEDLKIEKSRKATINRGKTKIAEKRRDVAMKKMGNQPSLALLFTKETISDWEDQPSPAKPKRIVLQWRSPAFTACSLKLDEMALTICKTTAERASIRKLLERDPVAITNVPVEFEAVPRKLPIDAYDSTFLSGLSPVEKEHLDMGDAIDLDSKVQTLNEMTLRGRGGAGIPLISRGNVGGLGVGGVQGPSSGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNI9</accession>
    <name>E3KNI9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_11620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178297">
        <property type="protein sequence ID" value="EFP85864.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003330235.2"/>
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    <dbReference type="AlphaFoldDB" id="E3KNI9"/>
    <dbReference type="GeneID" id="10544445"/>
    <dbReference type="KEGG" id="pgr:PGTG_11620"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11620"/>
    <dbReference type="HOGENOM" id="CLU_1603556_0_0_1"/>
    <dbReference type="InParanoid" id="E3KNI9"/>
    <dbReference type="OrthoDB" id="2508865at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013248">
        <property type="entry name" value="Psh3/Shr3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28228">
        <property type="entry name" value="SECRETORY COMPONENT PROTEIN SHR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28228:SF1">
        <property type="entry name" value="SECRETORY COMPONENT PROTEIN SHR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP85864.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="166" mass="18068" checksum="BE3DAEA10E062A89" modified="2012-04-18" version="2">MKCFCLFQAGLTVHHLEPIQTAFFSQSRLAPRRPSSNSDQGSNSAAVSKTSVLQFDLEPSVLHGESPPQKDGHQQEPSKVLQWLDPSDHHPSSWLAFCAGNLSESVDSNDAYDLVILPISVSTLSFHHHSPLLRPLLVAAVFPGILLGKTLVFIRKAGSSKLHSPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNZ9</accession>
    <name>E3KNZ9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FH2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11980</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178298">
        <property type="protein sequence ID" value="EFP86024.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330443.2">
        <property type="nucleotide sequence ID" value="XM_003330395.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNZ9"/>
    <dbReference type="GeneID" id="10540026"/>
    <dbReference type="KEGG" id="pgr:PGTG_11980"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11980"/>
    <dbReference type="HOGENOM" id="CLU_1627895_0_0_1"/>
    <dbReference type="InParanoid" id="E3KNZ9"/>
    <dbReference type="OrthoDB" id="2510166at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2220">
        <property type="entry name" value="Formin, FH2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042201">
        <property type="entry name" value="FH2_Formin_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86024.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="163" mass="18013" checksum="1DF00FFAA4A42439" modified="2012-04-18" version="2">MTDLQREMEELVRYFGQESNRLKPELLFGTLTRFKSEFEHTTAELGFDEFQEGDYSLENSFEVGQGNELNGQVEPEAGGTQTIFSSDKLPSTPIGQGRITGGTQSRRHTRGGTGSVVRSGVGKGGLDVAIREIRTGSKLRPLHFFQREVEDSPTPASRIFLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPB4</accession>
    <name>E3KPB4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_12095</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178298">
        <property type="protein sequence ID" value="EFP86139.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330558.2">
        <property type="nucleotide sequence ID" value="XM_003330510.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KPB4"/>
    <dbReference type="GeneID" id="10540147"/>
    <dbReference type="KEGG" id="pgr:PGTG_12095"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12095"/>
    <dbReference type="HOGENOM" id="CLU_135775_0_0_1"/>
    <dbReference type="InParanoid" id="E3KPB4"/>
    <dbReference type="OrthoDB" id="9254763at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172756" evidence="2">
        <location>
            <begin position="18"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="133"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP86139.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="155" mass="17379" checksum="E2DA873D0F86EC9F" modified="2012-04-18" version="2">MFHRIVTCSFVLFTCRAVDNFSGPSLRDFHDGTAGSLMPEATNSLNSPYHLAVSTSTNEAECHLNEKGSRVDLMFSTQRLESNEGDLTLARLSQPNYLEKQKQRYNSREQIEPKLNKGNYHGRDPIATCEMTSKSSSKFQKSSDYAKSKSSTALE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQG1</accession>
    <name>E3KQG1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12492</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178300">
        <property type="protein sequence ID" value="EFP86536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330955.1">
        <property type="nucleotide sequence ID" value="XM_003330907.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQG1"/>
    <dbReference type="STRING" id="418459.E3KQG1"/>
    <dbReference type="GeneID" id="10540635"/>
    <dbReference type="KEGG" id="pgr:PGTG_12492"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12492"/>
    <dbReference type="eggNOG" id="ENOG502R2PN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_837118_0_0_1"/>
    <dbReference type="InParanoid" id="E3KQG1"/>
    <dbReference type="OMA" id="LIWTRIP"/>
    <dbReference type="OrthoDB" id="2506056at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006044">
        <property type="term" value="P:N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022036">
        <property type="entry name" value="DUF3605"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35020">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-INDUCED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35020:SF2">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-INDUCED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12239">
        <property type="entry name" value="DUF3605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="332" mass="38564" checksum="B594FAECD53823A8" modified="2011-01-11" version="1">MLSNPTQQQPTIKPFLKNNNNNRTPTHERVTKTSRITEMNSAPNQHQQQQQDETNNIDNPYNETPSTEPFNIDPDLVRVLAKNQAIIARSPPRFVLFPSSSDHSTKQQQQEEEEERSLIHIISRTQLDRLGRLADSEQLYFNEFSRIIKLKYDSSCLYLQQRLNFPLHLENKLNKDGGRGQPAFFCADGRLQDRRDYRVIRNEWPYAIPRDYQHIVVWSRLPLLDPSQARTSAELKQAHHQGLSGFENLSPELVDRLTQAGLNPDLKLTPFPAHHPGVRGERAGLDAQIRAFIRTRWPYSRGFIDLLWFLNPVHLQSCPQLPHFHVFVKKTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3KRY1</accession>
    <name>E3KRY1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Acyl-protein thioesterase 1</fullName>
            <ecNumber evidence="2">3.1.2.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Palmitoyl-protein hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_13275</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Hydrolyzes fatty acids from S-acylated cysteine residues in proteins with a strong preference for palmitoylated G-alpha proteins over other acyl substrates. Mediates the deacylation of G-alpha proteins such as GPA1 in vivo, but has weak or no activity toward palmitoylated Ras proteins. Has weak lysophospholipase activity in vitro; however such activity may not exist in vivo.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>S-hexadecanoyl-L-cysteinyl-[protein] + H2O = L-cysteinyl-[protein] + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="3.1.2.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. AB hydrolase 2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.22" evidence="2"/>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EFP87056.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331475.2">
        <property type="nucleotide sequence ID" value="XM_003331427.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KRY1"/>
    <dbReference type="STRING" id="418459.E3KRY1"/>
    <dbReference type="GeneID" id="10542137"/>
    <dbReference type="KEGG" id="pgr:PGTG_13275"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13275"/>
    <dbReference type="eggNOG" id="KOG2112">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059676_0_0_1"/>
    <dbReference type="InParanoid" id="E3KRY1"/>
    <dbReference type="OrthoDB" id="2504171at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000716">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050565">
        <property type="entry name" value="LYPA1-2/EST-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003140">
        <property type="entry name" value="PLipase/COase/thioEstase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655:SF17">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02230">
        <property type="entry name" value="Abhydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="83"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipase/carboxylesterase/thioesterase" evidence="12">
        <location>
            <begin position="173"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="111"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="409"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP87056.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="432" mass="47684" checksum="37736911493B53A0" modified="2012-04-18" version="2">MGAGNTFDPFGQLVLKVQPIESSVEALTFKASFLKRSCNGDDLRSLLLSTERVSFKDTFSDSLYHPPRSIGISFRPPRNNIKFYLCIIVVAAALIVMICLTAIGFGGRKHTNTAATPNPNPDSDPSSIPPRERFTSTEYRANWTNPDVDVPQVAYDVIPPVDSINGRGLGWSVLFIHGLGALNASDGYRWRDILLSNYSEPQLTGNSFGNLTGVKFILPKAPIRPITVYAAQENRGARPGWFDIKDWRDLHYLEDEEGLRQSVIGLSTILKNEALAGRIQLNQTIIAGFSQGAVMSLLLTLTLPQPPAACVMMSGYLPLPLRLVDLRSASLDEYRKTSLYWLHGTDDAVLNYNQARAGMKLFSSLFSDRFLRAKFKTFLGLKHGFNMGEQKVVTNYIDGIVSRSTSGSFDSSLDASVEPENEGDVIQFNTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KS73</accession>
    <name>E3KS73_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13367</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EFP87148.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331567.2">
        <property type="nucleotide sequence ID" value="XM_003331519.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KS73"/>
    <dbReference type="GeneID" id="10542391"/>
    <dbReference type="KEGG" id="pgr:PGTG_13367"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13367"/>
    <dbReference type="HOGENOM" id="CLU_1918082_0_0_1"/>
    <dbReference type="InParanoid" id="E3KS73"/>
    <dbReference type="OrthoDB" id="2509321at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="87"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87148.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="132" mass="14538" checksum="7735E192403FEADB" modified="2012-04-18" version="2">MTTPLNDASAGEAYLTTHQTANQILAHRNPEAQLPPGILAAIQEIMVQSENRLGNALREALVELEQSAIRREDTLRASVVLSVIRSKTRLANQIAELRQDINSLQNSIRSMTDGTTQAMNRARDALPNQPNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KT39</accession>
    <name>E3KT39_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_13653</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178306">
        <property type="protein sequence ID" value="EFP87425.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331844.2">
        <property type="nucleotide sequence ID" value="XM_003331796.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KT39"/>
    <dbReference type="GeneID" id="10538154"/>
    <dbReference type="KEGG" id="pgr:PGTG_13653"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13653"/>
    <dbReference type="HOGENOM" id="CLU_1960670_0_0_1"/>
    <dbReference type="InParanoid" id="E3KT39"/>
    <dbReference type="OrthoDB" id="2514139at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP87425.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="128" mass="14538" checksum="7CC757789BBBC65B" modified="2012-04-18" version="2">MDIDPRDTKLAKLQEQMAKILAVVKEELKIRKDTKEQLAKEAQLATALKTASTSCTGQRPPTSLMALAELKQRSLITSDWNHLTNFKQNKKIIKNTLTKDHYFSGKQNKFINHICNNPTAYSVILLIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="103" xmlns="https://uniprot.org/uniprot">
    <accession>E3KTD2</accession>
    <name>E3KTD2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Pentafunctional AROM polypeptide</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="21">3-dehydroquinate synthase</fullName>
                <shortName evidence="21">DHQS</shortName>
                <ecNumber evidence="21">4.2.3.4</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="21">3-phosphoshikimate 1-carboxyvinyltransferase</fullName>
                <ecNumber evidence="21">2.5.1.19</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="21">5-enolpyruvylshikimate-3-phosphate synthase</fullName>
                <shortName evidence="21">EPSP synthase</shortName>
                <shortName evidence="21">EPSPS</shortName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="21">Shikimate kinase</fullName>
                <shortName evidence="21">SK</shortName>
                <ecNumber evidence="21">2.7.1.71</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="21">3-dehydroquinate dehydratase</fullName>
                <shortName evidence="21">3-dehydroquinase</shortName>
                <ecNumber evidence="21">4.2.1.10</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="21">Shikimate dehydrogenase</fullName>
                <ecNumber evidence="21">1.1.1.25</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="29" type="ORF">PGTG_13928</name>
    </gene>
    <organism evidence="29 30">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="30" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="30">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21 22">The AROM polypeptide catalyzes 5 consecutive enzymatic reactions in prechorismate polyaromatic amino acid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21 22">
            <text>3-dehydroquinate = 3-dehydroshikimate + H2O</text>
            <dbReference type="Rhea" id="RHEA:21096"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="EC" id="4.2.1.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21256"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="EC" id="2.5.1.19"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="19">
            <dbReference type="Rhea" id="RHEA:21257"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21 22">
            <text>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate = 3-dehydroquinate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21968"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="EC" id="4.2.3.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20 21 22">
            <text>shikimate + ATP = 3-phosphoshikimate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13121"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.71"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21 22">
            <text>shikimate + NADP(+) = 3-dehydroshikimate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17737"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="21 22">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="21 22">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="21 22">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 2/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="21 22">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="21 22">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 21 22">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 21 22">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="21 22">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="21 22">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the EPSP synthase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="21 22">In the 2nd section; belongs to the EPSP synthase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the 2nd section; belongs to the type-I 3-dehydroquinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="21 22">In the 3rd section; belongs to the shikimate kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="21 22">In the 4th section; belongs to the type-I 3-dehydroquinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="21 22">In the C-terminal section; belongs to the shikimate dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="22">In the N-terminal section; belongs to the dehydroquinate synthase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the shikimate kinase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="21">In the N-terminal section; belongs to the sugar phosphate cyclases superfamily. Dehydroquinate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.4" evidence="21"/>
    <dbReference type="EC" id="2.5.1.19" evidence="21"/>
    <dbReference type="EC" id="2.7.1.71" evidence="21"/>
    <dbReference type="EC" id="4.2.1.10" evidence="21"/>
    <dbReference type="EC" id="1.1.1.25" evidence="21"/>
    <dbReference type="EMBL" id="DS178307">
        <property type="protein sequence ID" value="EFP87557.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331976.2">
        <property type="nucleotide sequence ID" value="XM_003331928.2"/>
    </dbReference>
    <dbReference type="FunCoup" id="E3KTD2">
        <property type="interactions" value="103"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KTD2"/>
    <dbReference type="GeneID" id="10538617"/>
    <dbReference type="KEGG" id="pgr:PGTG_13928"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13928"/>
    <dbReference type="eggNOG" id="KOG0692">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001201_1_2_1"/>
    <dbReference type="InParanoid" id="E3KTD2"/>
    <dbReference type="OrthoDB" id="197068at2759"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00085"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003855">
        <property type="term" value="F:3-dehydroquinate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003856">
        <property type="term" value="F:3-dehydroquinate synthase activity"/>
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            <position position="128"/>
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        <ligand>
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        <location>
            <begin position="137"/>
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        <ligand>
            <name>NAD(+)</name>
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            <position position="150"/>
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        <ligand>
            <name>7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate</name>
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            <name>NAD(+)</name>
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    </feature>
    <feature type="binding site" evidence="21">
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            <position position="192"/>
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        <ligand>
            <name>Zn(2+)</name>
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    <feature type="binding site" evidence="21">
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            <position position="230"/>
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            <name>Zn(2+)</name>
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        <location>
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        <location>
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        <ligand>
            <name>ATP</name>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00044633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="PIRNR" id="PIRNR000514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF00275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="Pfam" id="PF01488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="25">
        <source>
            <dbReference type="Pfam" id="PF01761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="Pfam" id="PF08501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="27">
        <source>
            <dbReference type="Pfam" id="PF18317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="28">
        <source>
            <dbReference type="Pfam" id="PF24621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="29">
        <source>
            <dbReference type="EMBL" id="EFP87557.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="30">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1620" mass="176393" checksum="A7E1B87FC34E3A4A" modified="2012-04-18" version="2">MAHSVCDVQTVSILGKPSIRLGYHLAPYIAHTVLTELHSSTYVLITDSNVSPRHVPALKAAFENQLPKSARLLTYILPPGEQSKSREMKAVLEDWLLDHRCTRDTVVLALGGGVIGDLIGFVSATFMRGIRFCQIPTTLLAMVDSSVGGKTAIDTPLGKNLVGAFWQPSFIFIDASYLETLPEREFVNGMAEMIKTAAIWDESQFENWNPTRSQAQKLLLQVIADSVGVKSEVVTKDEKETGLRNLVNFGHTIGHAIEAVLTPAILHGECVSIGMILEAEVARARGCLSSSAIARLSKCLKAHGLPVSLTDPRILSSPNAPNLNLDRLLDLMSVDKKNEGKIKKIVLLSRIGKTFEERATGVEDSLIRRVLAPAVRVHPGPPPANIKEVTLRTPGSKSISNRALILAALGHGTCRLKNLLHSDDTQVMINALEEMKGASFSWEDNGATLVVSGGAGKLSSPANGKHVYLGNAGTAARFLTTVCSLVKSQVSNQSSTVITGNARMQERPIGPLVDALRTNGVQIDYLRNEGCLPLRISPEDGFPGGMIELAASVSSQYVSSVLLSAPFAQAPVTLSLVGGTVISQPYIDITISMMSTFGIQVERLNNPATGLPSNTYRIPNGTYKNPPVYEIESDASSATYPLAMAALNGLSITLETIGSGSLQGDAQFAKKVLEPMGCQVIQTERETKVIGPSTVSELRQLGEIDMEEMTDAFLTACVVLGVAVQPSEKEQKMSTRIIGIANQRVKECNRIAAMVAELGKMGIHAQELEDGIEVFGTPVDALAKRGDQVRINCYDDHRIAMAFSVLGTVPGGKGLILNEKRCVEKTWPSWWDDLSTKLGIQLDGVPAHEKLTNTTSAVAWEAKKPETPSILLCGMRGSGKSFSGQVAASTLGWPLIDTDLYFQEKCGCSIREFIQKNDWSHFREEECAVLKEILKEFPSRHVISLGGGIVETEKGRKMLMDYANSKGPVVEIIRPVEEVIAYLNEDGNRPSLGESIEQIWARREPWYRLCSNSEYFNLVHPTIDHALISEVQHAKRAMRQFFRFITGVDTNHVLLDPPVPRNTHFLCLTFPSLSPCREEDKNILDRFEELTVGCDAVELRVDLLSTSGKAPTSPSIPPTEFVVKQVAALRRLTTLPIIYTVRTCSQGGMFPDGQPEEWCKLAAMGFRTGCEYVDIELGLSPEQQKALCSQKGHSKIIASYHDFSGRLKWDSSEMIQRYHELKAYGDMVKLVSRAADGLEDNLAMLEFRKKHATLQKDRQSLITINMGRSGQMSRILSPVLSPTTHPLLPGPPAAPGQLSFRQIQTAQGLLGQIIPKQFWLFGAPIGQSRSPLIHGTGFDTLGLEGYRYQKCETSSVTDSTVLEKIHASDFGGASVTIPLKVEIIKYLDQLTPDAQAVGAVNTIVPVSQRNGKIKLLGANTDWEAIKTKIEANLPAQQALGLITPSQLVRAAGVVIGAGGTARAAVYALHSLGYEQIYIHNRTRSKAEEVAKAFPSYFGIRVIDSLGFLCSGEEGVWQPSAIVSAVPAQATRLSDDPPSDRLEIPRGLFDRPGGGVVIEMSYSLGKDSPLLRAVQDLVGWKSVDGLELLVEQGARQFQLWTGKHLSMKIVSQAVYQEASSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KTP7</accession>
    <name>E3KTP7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_13534</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178308">
        <property type="protein sequence ID" value="EFP87748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332167.1">
        <property type="nucleotide sequence ID" value="XM_003332119.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KTP7"/>
    <dbReference type="STRING" id="418459.E3KTP7"/>
    <dbReference type="GeneID" id="10539146"/>
    <dbReference type="KEGG" id="pgr:PGTG_13534"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13534"/>
    <dbReference type="HOGENOM" id="CLU_1294976_0_0_1"/>
    <dbReference type="InParanoid" id="E3KTP7"/>
    <dbReference type="OrthoDB" id="2150628at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051667">
        <property type="entry name" value="Archaeal_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37096:SF1">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37096">
        <property type="entry name" value="YALI0E33429P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP87748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="213" mass="24317" checksum="DCF2F5E97E833F65" modified="2011-01-11" version="1">MPLRVFHLKITEITVASEQLHADQPKDVEKLTRLLMGEPQFTVMLGPPNSGKTALARHGSFLEAFRDEGSLARAQNVFYERLFVKSKTTSGETSRQASRNQTFRTKRAKTFKELGSHLKPWPLSHGGRPPVLIIDEENVFKRMNDDMTGGKMFLIKSYIPQVYEYGHFQDLQLPHFARDLDPCHSLPTLTALSEPALRAMERLIEDLKQEVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3KWR5</accession>
    <name>E3KWR5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Retrotransposon Copia-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_14698</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178316">
        <property type="protein sequence ID" value="EFP88732.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333151.2">
        <property type="nucleotide sequence ID" value="XM_003333103.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KWR5"/>
    <dbReference type="GeneID" id="10541683"/>
    <dbReference type="KEGG" id="pgr:PGTG_14698"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14698"/>
    <dbReference type="HOGENOM" id="CLU_051119_2_0_1"/>
    <dbReference type="InParanoid" id="E3KWR5"/>
    <dbReference type="OrthoDB" id="2498930at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027532">
        <property type="entry name" value="Mdm12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204:SF1">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="243"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="275"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP88732.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="291" mass="33444" checksum="37F59595286A32B7" modified="2012-04-18" version="2">MATEIVRESKILLNNDNYALWLLPMKAKLHKAKSLTIVTGIHTCPDPEKDKENAKLYVKLNEDAYAEIVQHLNQEVLAYVSSTQTETDEFNGYKLWQLLKQKYAGNDLTSRTTALKKYLAVEYDSFSAFLPLIRSANQKIVLSKLALDDQVKTILMLDKLPPEFHSFKTNISMNFETESFEEVLKKLEDFAAQNQLNELKKRSTSPISSQATMYTRSSDPDIVCPHCKRGFRVCSHCFKSGHTEDSCYQKHPKKRAPKSVASSSKQHSSHLTRYTQEDKEYLQQKYPDLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KWW1</accession>
    <name>E3KWW1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_14744</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178316">
        <property type="protein sequence ID" value="EFP88778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333197.1">
        <property type="nucleotide sequence ID" value="XM_003333149.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KWW1"/>
    <dbReference type="GeneID" id="10541729"/>
    <dbReference type="KEGG" id="pgr:PGTG_14744"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14744"/>
    <dbReference type="HOGENOM" id="CLU_174214_0_0_1"/>
    <dbReference type="InParanoid" id="E3KWW1"/>
    <dbReference type="OrthoDB" id="2512272at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173801" evidence="2">
        <location>
            <begin position="23"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="88"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP88778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="110" mass="10824" checksum="664A9F184221A475" modified="2011-01-11" version="1">MQASSILCCLAFLALTHQGVLSMHEAGSSASKAKQAGKAPMEAPPTPARSSGVPGSNPNSVTEAFRMILDSPKDTDISGHQSGGPSGSGSSSGNDAGSSGSRSGGGHGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KWY3</accession>
    <name>E3KWY3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14215</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178317">
        <property type="protein sequence ID" value="EFP88876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333295.1">
        <property type="nucleotide sequence ID" value="XM_003333247.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KWY3"/>
    <dbReference type="GeneID" id="10541992"/>
    <dbReference type="KEGG" id="pgr:PGTG_14215"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14215"/>
    <dbReference type="HOGENOM" id="CLU_758966_0_0_1"/>
    <dbReference type="InParanoid" id="E3KWY3"/>
    <dbReference type="OrthoDB" id="2498953at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="53"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="320"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="365" mass="41373" checksum="1B57D018B4F9E7DF" modified="2011-01-11" version="1">MKRRCSSSGEPRGTRPSKRLRSKSHSEPRQKQSQPIDSAAKAPAENQDEDQAPAEAQEGAEANDNQALEEAVDKRITQGIRRYERDDHFERDIWDTLMQLAHQQSQNLSRSLVHWGRKGGLRDTRTSKSSANKDPKPSNPAPENLAANTDESQNLTYDPTATSFTLWPLPTQACPIPEWTLNEELCSLVTRIKRERSRNEGTDSPPIDHELEDEKAQSLADQNQKTLMQLLNDLYEFHPPHTSSIPIYTRGKTYRKLDKAAKNKSKTDQSTDPKNEKEVDFGLNWEFVLGVASSSTFDIKESVLQRARERLTEMYRVTSEPANGQATDDPLQTSQAVSDPIQHLQRYREMKPLALSSDESSYHSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3KX07</accession>
    <name>E3KX07_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Uracil-DNA glycosylase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_14239</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the uracil-DNA glycosylase (UDG) superfamily. UNG family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178317">
        <property type="protein sequence ID" value="EFP88900.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333319.2">
        <property type="nucleotide sequence ID" value="XM_003333271.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KX07"/>
    <dbReference type="FunCoup" id="E3KX07">
        <property type="interactions" value="430"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KX07"/>
    <dbReference type="GeneID" id="10542178"/>
    <dbReference type="KEGG" id="pgr:PGTG_14239"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14239"/>
    <dbReference type="eggNOG" id="KOG2994">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032162_2_2_1"/>
    <dbReference type="InParanoid" id="E3KX07"/>
    <dbReference type="OrthoDB" id="10031947at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097510">
        <property type="term" value="P:base-excision repair, AP site formation via deaminated base removal"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd10027">
        <property type="entry name" value="UDG-F1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002043">
        <property type="entry name" value="UDG_fam1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018085">
        <property type="entry name" value="Ura-DNA_Glyclase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005122">
        <property type="entry name" value="Uracil-DNA_glycosylase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003592">
        <property type="entry name" value="PRK05254.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00628">
        <property type="entry name" value="ung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11264">
        <property type="entry name" value="URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11264:SF0">
        <property type="entry name" value="URACIL-DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03167">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00986">
        <property type="entry name" value="UDG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00987">
        <property type="entry name" value="UreE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00130">
        <property type="entry name" value="U_DNA_GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uracil-DNA glycosylase-like" evidence="7">
        <location>
            <begin position="157"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="286"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="28"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="346"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP88900.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="483" mass="52310" checksum="7043046388E2C569" modified="2012-04-18" version="2">MAPAAASKPDKRQRSIAQMFGAKDPTLAATSKSSSQGASSSTTSSSRITAPAPKAKNKASNVKTLMTAGRLARVSYQKLQPIDPESYRDSLSAEFKELLALELDPEHGLGPTYLHVLRAQLTQQYFLDLKRFLIAEGLNQPRNDIYPTSKNIYHWSRATPLDQIRVVIIGQDPYHGPGQAHGLSFSVPKGIPIPGSLRNIYQELKSEYPEFKAPGHGTLRSWAESGVLLLNTSLTVKKGAAGSHSNRGWEQFTDAVVDAIDLYGGLGLLNNQKPSEVDQIVEPASKRMKLDHPPSTEASPPQAKGPETGSDSDPKAPSPIAYSTPMASISDPKHTTSAPEPEKTNLKPSTEDLNTLASVDKKSEESQPARPDTNSAESGSKAKPPEVAGADVEPPSTKLTEEPKPQDLPGFGKGVVFLCWGKWAADRVARLSESKHLILKSAHPSPLSAHRGFLGNKHFKLANEWLENKYGESAKINWCDLKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3KY36</accession>
    <name>E3KY36_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178320">
        <property type="protein sequence ID" value="EFP89237.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333656.2">
        <property type="nucleotide sequence ID" value="XM_003333608.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KY36"/>
    <dbReference type="STRING" id="418459.E3KY36"/>
    <dbReference type="GeneID" id="10546874"/>
    <dbReference type="KEGG" id="pgr:PGTG_15078"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15078"/>
    <dbReference type="HOGENOM" id="CLU_1482700_0_0_1"/>
    <dbReference type="InParanoid" id="E3KY36"/>
    <dbReference type="OrthoDB" id="10527924at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324:SF2">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="102"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="119"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89237.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="219" mass="24040" checksum="89887ACF139E4BD3" modified="2012-04-18" version="2">MVFLGITQKISDLQSSYNTARDWKRNTGAGILKSDGVNGVKTVETKSTSYADTGTFLIQLWDQEGQANDSTSGSTVNNQSNLPSSGRDATCSDDEAGQETITQTPTPAATASTTQVTTQKKKKKKKKSTTPLSKRNGKGRKKTNPEDFYMKSVITKRQAEMTKAQAAATQAKVSYMKELRELGLEYQEIKQLVDEEFPTIPDMLADSEEDESDSDEDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3KYA6</accession>
    <name>E3KYA6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15476</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178321">
        <property type="protein sequence ID" value="EFP89297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333716.1">
        <property type="nucleotide sequence ID" value="XM_003333668.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KYA6"/>
    <dbReference type="GeneID" id="10547017"/>
    <dbReference type="KEGG" id="pgr:PGTG_15476"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15476"/>
    <dbReference type="HOGENOM" id="CLU_140512_0_0_1"/>
    <dbReference type="InParanoid" id="E3KYA6"/>
    <dbReference type="OrthoDB" id="2508824at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174019" evidence="1">
        <location>
            <begin position="24"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="164" mass="18372" checksum="C17B80485917E6D3" modified="2011-01-11" version="1">MKMCFAELLFCLASHLTFNLATGMITQWECARLCSPKYSNIRVYDANGAECGLIHKCSGGMSHGKCKQFRQMDMGYCNSCTGIHMMFQEKDCPLELKLQDHCDTHPCQTTAIQGSNVEHGSNQHNSHQEFCSLNAIKINEISFVLQLKCYYNPTTMTHLCATGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZ08</accession>
    <name>E3KZ08_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15682</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178323">
        <property type="protein sequence ID" value="EFP89533.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333952.2">
        <property type="nucleotide sequence ID" value="XM_003333904.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZ08"/>
    <dbReference type="GeneID" id="10547501"/>
    <dbReference type="KEGG" id="pgr:PGTG_15682"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15682"/>
    <dbReference type="HOGENOM" id="CLU_1161627_0_0_1"/>
    <dbReference type="InParanoid" id="E3KZ08"/>
    <dbReference type="OrthoDB" id="2500617at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89533.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="239" mass="27451" checksum="CE58C79BEE73DF3E" modified="2012-04-18" version="2">MLDINLLLRIRPSDRSLDLPDCSRGDGERSAPEEPPEEGPQSQERIRRLTETILAVDRPRMVSGTLAEDASSGKIPEFKFIHLFRSSSHSPHDRQHWFQWLDHINNIDQHLDKITLDSIPNDLLQAEQWSSQRILQKPEPLPLNRDSSGSFHPTHSKSPSSDKTLKSPIQEHQTLEKRDENHQQSAAEHQPHEIRFDTINAGLGLTECNLMESQLGESYELDDITRFVRAEDQDYLSQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZT9</accession>
    <name>E3KZT9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">26S proteasome regulatory subunit 7 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_15776</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178326">
        <property type="protein sequence ID" value="EFP89820.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334239.2">
        <property type="nucleotide sequence ID" value="XM_003334191.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZT9"/>
    <dbReference type="FunCoup" id="E3KZT9">
        <property type="interactions" value="585"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KZT9"/>
    <dbReference type="GeneID" id="10545551"/>
    <dbReference type="KEGG" id="pgr:PGTG_15776"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15776"/>
    <dbReference type="eggNOG" id="KOG0729">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_6_0_1"/>
    <dbReference type="InParanoid" id="E3KZT9"/>
    <dbReference type="OrthoDB" id="1937997at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008540">
        <property type="term" value="C:proteasome regulatory particle, base subcomplex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036402">
        <property type="term" value="F:proteasome-activating activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000005">
        <property type="entry name" value="26S protease regulatory subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000027">
        <property type="entry name" value="26S protease regulatory subunit 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000290">
        <property type="entry name" value="Probable RPT1-26S proteasome regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048723">
        <property type="entry name" value="OB_PRS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21236">
        <property type="entry name" value="OB_PRS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-0647">Proteasome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="10">
        <location>
            <begin position="249"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="108"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00067449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP89820.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="474" mass="52399" checksum="406997DF07A0972D" modified="2012-04-18" version="2">MPPKQDWEKSKKTSDDIAATDDIIALDEGDIQLLKTYGQGPYARQLRTIEKDLQDLQKRIGEKMGVKESDTGLAPPNLWDTAVDRVRMSQETSCLQVARCTKIIRAPDANATPEDEASRRANGRQPAGAGAGGRTLPGAGMSRPNADEEDKYVINVKQIAKFVVGLGEQMAPTDIEEGMRVGVDRNKYQIQIPLPPKIDPSVTMMQVEEKPDVTYSDVGGCKEQIEKLREVVETPLLNPEKFVALGIDPPKGVMLFGPPGTGKTLCARAVANRTDATFIRVIGSELVQKYVGEGARMVRELFEMARTKKACIIFFDEVDAIGGARHDDGQGGDNEVQRTMLELINQLDGFDPRGNIKVIMATNRPDTLDPALLRPGRLDRRVEFSLPDNEGRANILKIHARSMSVERNIRYELIARLCPNATGAELRSVCTEAGMFAIRARRKVANEKDFLDAVEKVIRQGSKFSSTALYQNYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3L0S3</accession>
    <name>E3L0S3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15975</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178329">
        <property type="protein sequence ID" value="EFP90127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334546.1">
        <property type="nucleotide sequence ID" value="XM_003334498.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L0S3"/>
    <dbReference type="GeneID" id="10546185"/>
    <dbReference type="KEGG" id="pgr:PGTG_15975"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15975"/>
    <dbReference type="HOGENOM" id="CLU_1856289_0_0_1"/>
    <dbReference type="InParanoid" id="E3L0S3"/>
    <dbReference type="OrthoDB" id="10385345at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="138" mass="15875" checksum="23CCA77D03959D4E" modified="2011-01-11" version="1">MHFTREAYWRFCSHWLGINPLKRTIGTRVPLVVPWYRLHFAQKWTPGTSPGTPWQEPGGSGPKIQLIKREHSSREFSQMSNSGYSSELSDTEAKIPEPDSSFISTEISEGELKKYIENVNPLYKKDILFPDSKKLESC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3L192</accession>
    <name>E3L192_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16313</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178331">
        <property type="protein sequence ID" value="EFP90287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334706.1">
        <property type="nucleotide sequence ID" value="XM_003334658.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L192"/>
    <dbReference type="GeneID" id="10530181"/>
    <dbReference type="KEGG" id="pgr:PGTG_16313"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16313"/>
    <dbReference type="HOGENOM" id="CLU_1195388_0_0_1"/>
    <dbReference type="InParanoid" id="E3L192"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="232" mass="27102" checksum="943DE8A72D17FB22" modified="2011-01-11" version="1">MSRTRSHHPTLVEAQDLTGTPRGRHSDKVFFKRAKKTNNECLKVGALSAALNLQTNLLRLISQEEFLEMFKWDPNSEFEEYANGEKGRQFLKERGTKVTPTPPPKVKMQPPQSAQSQEQRTENYPDYQPQPPMYYHPNRYYYPYPPEMYHQQNPSWNQPGYYHNNQMYPVAHQHPVQENLQSQVPQQPPGSTNQKNQNDQAQKSNRDRLHNRKAKGPNWVPPPTLKRAPQDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>E3L1W2</accession>
    <name>E3L1W2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Histone-lysine N-methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_16563</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178334">
        <property type="protein sequence ID" value="EFP90537.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334956.2">
        <property type="nucleotide sequence ID" value="XM_003334908.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L1W2"/>
    <dbReference type="STRING" id="418459.E3L1W2"/>
    <dbReference type="GeneID" id="10530435"/>
    <dbReference type="KEGG" id="pgr:PGTG_16563"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16563"/>
    <dbReference type="eggNOG" id="KOG1083">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_380384_0_0_1"/>
    <dbReference type="InParanoid" id="E3L1W2"/>
    <dbReference type="OrthoDB" id="308383at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3L398</accession>
    <name>E3L398_PUCGT</name>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP91023.2"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3R5</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="2" type="ORF">PGTG_16381</name>
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        </lineage>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
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</entry>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
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                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
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    </evidence>
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        <source>
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</entry>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
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        <source>
            <dbReference type="EMBL" id="EFP91416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="17">Upf1 domain-containing protein</fullName>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
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                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
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                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
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    <reference evidence="19" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">RNA-dependent helicase required for nonsense-mediated decay (NMD) of aberrant mRNAs containing premature stop codons and modulates the expression level of normal mRNAs. Also capable of unwinding double-stranded DNA and translocating on single-stranded DNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="DS178362">
        <property type="protein sequence ID" value="EFP92300.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336719.2">
        <property type="nucleotide sequence ID" value="XM_003336671.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L6X5"/>
    <dbReference type="FunCoup" id="E3L6X5">
        <property type="interactions" value="996"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L6X5"/>
    <dbReference type="GeneID" id="10537961"/>
    <dbReference type="KEGG" id="pgr:PGTG_17974"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17974"/>
    <dbReference type="eggNOG" id="KOG1802">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001666_4_0_1"/>
    <dbReference type="InParanoid" id="E3L6X5"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd21407">
        <property type="entry name" value="1B_UPF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18039">
        <property type="entry name" value="DEXXQc_UPF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21400">
        <property type="entry name" value="ZBD_UPF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000097">
        <property type="entry name" value="Regulator of nonsense transcripts 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045055">
        <property type="entry name" value="DNA2/NAM7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040812">
        <property type="entry name" value="UPF1_1B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018999">
        <property type="entry name" value="UPF1_CH/ZBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10887:SF364">
        <property type="entry name" value="REGULATOR OF NONSENSE TRANSCRIPTS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18141">
        <property type="entry name" value="UPF1_1B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09416">
        <property type="entry name" value="UPF1_Zn_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51997">
        <property type="entry name" value="UPF1_CH_RICH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0347">Helicase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0866">Nonsense-mediated mRNA decay</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 15" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 15" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Upf1" evidence="17">
        <location>
            <begin position="115"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="63"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="C4" evidence="15">
        <location>
            <begin position="187"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="931"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1033"/>
            <end position="1060"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1075"/>
            <end position="1116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="74"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="949"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="1033"/>
            <end position="1043"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00055561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EFP92300.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1131" mass="124933" checksum="8B9B281831C87E05" modified="2012-04-18" version="2">MENFSHLGGASEYSYNPANDDDDSLSLVSGSTRAASQTNRHQVEGDGQSTIDDINFDELSLLDANPEPSRNRHQANNNSSNYDHQSHSDSATEQSSKFNQAENEADFEDVLDDLNRELPNHACAYCGIHSPSSVVKCLICSKWFCNSRGGPGAHSTGSHIVNHLVRAKHKEVTLHEDGPLGETTPECYNCGSKNVFTLGFIPAKSDTVVVLLCRQPCASTPSSKSDLVWDTSQWSPLIEDRQFLSWLVKVPDEQEVVRSKPVGFREITRLEEMWKENATATLDDLERDGALGEGEPQPILLRYEDAYQYQNIFGPLVKIESDYDRKLKESLTQNDVTVRWDMGLNQKRIAYFYLPKLESGEVRLAVGDELRLKYAGELQKAWEGPGHVIKVPNNQSDEVALELRRNDGVPHELTVNFSVDFVWKSTSFDRMQMAMKTFAVDDQSVSGYIYRKLLGHETPVPQVLRTQMPKRVSAPNLPELNHSQVSAVKSVLQKPLSLVQGPPGTGKTVTSASVVYHLSKMNPGQVLVCAPSNVAVDQLTEKIHLTGLKVVRVQAKSREAIDSSVQYLTLHQQVANNDTHPDLQKLIQLKAEQGELSSTDERRYKSLTRLCEREILTNADVILCTCVGAGDPRLAKMKFRTVLIDEATQATEPECMIPLTLGVKQVVFVGDHQQLGPTIMNKKAARAGLTQSMFERLVLLGNRPIRLQVQYRMHPCLSEFPSNMFYEGSLQNGVTAPERIKKNVDFPWPQPTTPMCFHCNLGQEEISSSGTSFLNRTEASNVEKVVTRFFKAGVLPSQIGIVTPYEGQRSYIVTYMQTNGTLKKDLYKDIEVASVDAFQGREKDYIILSCVRSNDHQGIGFLNDPRRLNVALTRAKYGVVVLGNPKVLSKHALWHFLLTAYKEKSCLVEGPLNNLQASLVQLSKPRKPLDRADLPVNRFEPNPRELPNLSYSRNGRDSTPTRFDQSFYKTVDSFSIPSDAQSVRSQATYASGLPPFSLPISRSMGYPNGSKRGGGGGMGSVVGVGGSSYASSMISQDFQSQDSRSMRGGGDDASSVAESMTDSVAAYSQADRLLAGRSRNDRTGLDYRGPGVSGLSSATVAGAGGKNFRAVDNDDDARSLISMQSSSITDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3L796</accession>
    <name>E3L796_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_18246</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178364">
        <property type="protein sequence ID" value="EFP92421.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336840.2">
        <property type="nucleotide sequence ID" value="XM_003336792.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L796"/>
    <dbReference type="GeneID" id="10538246"/>
    <dbReference type="KEGG" id="pgr:PGTG_18246"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18246"/>
    <dbReference type="HOGENOM" id="CLU_1627889_0_0_1"/>
    <dbReference type="InParanoid" id="E3L796"/>
    <dbReference type="OrthoDB" id="2509270at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP92421.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="163" mass="18423" checksum="E383EE91F735C9A8" modified="2012-04-18" version="2">MHQLTGYVDFGNQLEKFHSYLKGPTQNRLFQLKGKHGHITAIRPHRSTNKPGLGSSRRNLAPEILKYRILENNSNSSVEKFKTHQPVILEGDLLDAVNDKVTEELGHIWKSLQIQLKNLHSQSQASENSSANLVPLRCLLLLANLVHQHDFLPSISNQGTTIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8R5</accession>
    <name>E3L8R5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_18953</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178377">
        <property type="protein sequence ID" value="EFP92940.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337359.2">
        <property type="nucleotide sequence ID" value="XM_003337311.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L8R5"/>
    <dbReference type="GeneID" id="10542832"/>
    <dbReference type="KEGG" id="pgr:PGTG_18953"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18953"/>
    <dbReference type="HOGENOM" id="CLU_3051425_0_0_1"/>
    <dbReference type="InParanoid" id="E3L8R5"/>
    <dbReference type="OrthoDB" id="10336576at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP92940.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="54" mass="5758" checksum="80DDB90C96CC08BC" modified="2012-04-18" version="2">MLGLALAPLCGSSFQTPPIFSSIRSADDPQANPTIDDPRASRLSFPAVIFKLLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3NY06</accession>
    <name>E3NY06_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_20411</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS990005">
        <property type="protein sequence ID" value="EFP94455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338874.1">
        <property type="nucleotide sequence ID" value="XM_003338826.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NY06"/>
    <dbReference type="GeneID" id="10527746"/>
    <dbReference type="KEGG" id="pgr:PGTG_20411"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20411"/>
    <dbReference type="HOGENOM" id="CLU_050263_0_0_1"/>
    <dbReference type="InParanoid" id="E3NY06"/>
    <dbReference type="OrthoDB" id="2505769at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="236"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP94455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="489" mass="54991" checksum="B084AE568C6420D0" modified="2011-01-11" version="1">MTRTKRKHLLRALKRDGPLPLGGPDRLTMGNSINRQGHKGACAVIQPALKTATCIDGQSESPLSLGSTDLLARRKANNHQGYKEACVVSLPINKDASLPATGPLPTSSPDILEQRQDNKPQLQKKRRAVSSSTARAMSHRVHKPRNPPPQYASATAKKIARAYRKLKPSDDSYFIICSPSKARVSRKPIINLPVLNKPPRQIVLSSSLAPHFIFETHSFDVNPPTPTFTKLCEVIETLHAMAKYRPCNKRNANRLNGEMHLIGFRPGNDRGKSAGTYARRTDLTPAQSAHDDTLWAKLQSFNKFLSQRMEAISHSAYQQNKELMNKRGIPNWSQEEWAEMRKEDQIEFQFASNVSVTYNDFYNKPHQDKKDINGWTYGIFASVDLETGKPIPPPSTELGHGFLFPCHAYLIDFVKSSGIMELVWQTTKFEHCTTMPPPSLRKQKGRTWTHQGCSFQINKDIAKVAQELKDASPQFVEKKTLCKKQKYGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPX4</accession>
    <name>H6QPX4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20924</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EHS64312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890468.1">
        <property type="nucleotide sequence ID" value="XM_003890419.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPX4"/>
    <dbReference type="GeneID" id="13543010"/>
    <dbReference type="KEGG" id="pgr:PGTG_20924"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20924"/>
    <dbReference type="HOGENOM" id="CLU_003292_5_0_1"/>
    <dbReference type="InParanoid" id="H6QPX4"/>
    <dbReference type="OrthoDB" id="2503657at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052925">
        <property type="entry name" value="Phage_Integrase-like_Recomb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34605:SF3">
        <property type="entry name" value="P CELL-TYPE AGGLUTINATION PROTEIN MAP4-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34605">
        <property type="entry name" value="PHAGE_INTEGRASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="366" mass="40805" checksum="28052046167D2DCA" modified="2012-04-18" version="1">METGTIIDSSALTINHLNKIKAFVAKGTTHHPVSALNAHLLSGWKWNTLIGYNAAVKKFIFFKNLSGDHDFVLPATSKDIEDFCFWAGRNLYLNNSYKISASSIRKYLCGIKAWHTFHDTLYPEIADKRIELLLKASAKVDATKPLKPKKPAIMLKHLIWLSNMLFPGGKADKAIMDLAIVAFWGMARIGELTYSKPTGSIESQLSLLTTDARIVNSAIGEKVILTVRNAKTATPGKPQEIVLHSLKNMLCPVLAIKRRLEEAAGQKTSLFGFFCGDTRKHLTRSIAVNRIQLVLRSGGFDGLLGHSFRVGGASLRFALGTPVDEICVLGRWVLNCYKLYIREYDNSSLLESKKIIEELNELWDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPZ4</accession>
    <name>H6QPZ4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_20979</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EHS64517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890431.1">
        <property type="nucleotide sequence ID" value="XM_003890382.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPZ4"/>
    <dbReference type="GeneID" id="13542671"/>
    <dbReference type="KEGG" id="pgr:PGTG_20979"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20979"/>
    <dbReference type="HOGENOM" id="CLU_119658_0_0_1"/>
    <dbReference type="InParanoid" id="H6QPZ4"/>
    <dbReference type="OrthoDB" id="2496730at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="148" mass="16161" checksum="0029C96976F00087" modified="2012-04-18" version="1">MTVTVRLMKSGGPMVPSWRIKEKESGRPTRELTADKGICETYTNTTRGACLWIGDNPRTPTPKGLTPGWLTDDDKSNCGKQFIIKQGKKHVRGKIVDGCGFAEDGPPVTTAQGCSAIYVTKVLYTELGGNVDDKNDPGKVEIEAWDLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQ93</accession>
    <name>H6QQ93_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EHS64779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890300.1">
        <property type="nucleotide sequence ID" value="XM_003890251.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQ93"/>
    <dbReference type="STRING" id="418459.H6QQ93"/>
    <dbReference type="GeneID" id="13542168"/>
    <dbReference type="KEGG" id="pgr:PGTG_21039"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21039"/>
    <dbReference type="HOGENOM" id="CLU_520819_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQ93"/>
    <dbReference type="OrthoDB" id="185373at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="499"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="595"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="499"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="599"/>
            <end position="618"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="632" mass="71868" checksum="EF0F0B8AB1CF9432" modified="2012-04-18" version="1">MNNLRRKLLLLNTNNRRLISTATIKPLPPPPTLNLPVTIKPPPQKQIINLRYANNPSELIIRIEKLARDQRLDLIIQLIKSSSIRVATISVWTELFKLLIQLNRQNLAYKLFLELKRRQIKPDLKFFHLYFHLISTAERSQKHFSLERLTSLWNQSQQSINNNNNNNTTTTPEDHQASIISLTNAYLNCLLHHGYHPEAFKIFNSLKTLDSSTLYQISRSLSGTKEDLQRARELIERFKKEDQEPSLLDLRTTLSLANLFLKSNDTSDHQYAADLIQERIGIQLADNPYKFWAKTKPQPILGHLSSSEKSDSSKTMIRFEPGQLTTLLRMLLKMNKFALVRRVWTQISTNPDLYLQRDTIDSTHCGLVMIAMGRCGAMDSVKGLLRWMIESGNKRLRPTGDTLDKAIQAAWQTEDVSGGISVLASLTQTHTELMEPEEIAQDGLVELIQKTRHLAPLTPSNRALATLLQAAGRAGKIAEIGRALEAVARFRSLPDPTPAAALAAPGAPKQTAPFGGPESSAEIERYWVDQFIWVLTDLLDKLLARNDRSKVFSDSQIEKFEDWRSLIDRSLASDPLASSLRSRIEVRAQERLDWRTLQSASPSRRPSSHSSSHSFSRVSSHRRSTSDYRRRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQD2</accession>
    <name>H6QQD2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21092</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EHS62544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890257.1">
        <property type="nucleotide sequence ID" value="XM_003890208.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQD2"/>
    <dbReference type="GeneID" id="13541206"/>
    <dbReference type="KEGG" id="pgr:PGTG_21092"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21092"/>
    <dbReference type="HOGENOM" id="CLU_2347727_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQD2"/>
    <dbReference type="OrthoDB" id="10439852at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="26"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="97" mass="10284" checksum="364CB12A31885C77" modified="2012-04-18" version="1">MAEDIVTRATRKMKGLTRPGVKEPDTQPQTQTATAQSMSPQGPTQTTAGENTTNESSEHLSKRSNEVIDLSQVGVDKRTPPPSPHAHAPAPPKGACN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQL7</accession>
    <name>H6QQL7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21177</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EHS62669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890215.1">
        <property type="nucleotide sequence ID" value="XM_003890166.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQL7"/>
    <dbReference type="GeneID" id="13541286"/>
    <dbReference type="KEGG" id="pgr:PGTG_21177"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21177"/>
    <dbReference type="HOGENOM" id="CLU_2980150_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQL7"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS62669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="58" mass="6838" checksum="490CFFB171BE87B2" modified="2012-04-18" version="1">MTIPLRNPQSTGPSRSFKITLLKVWTWTTWSPDSAYWKMRLRQTPSWLFEITLTARPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>H6QR56</accession>
    <name>H6QR56_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GPN-loop GTPase</fullName>
            <ecNumber evidence="7">3.6.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_21323</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Small GTPase required for proper nuclear import of RNA polymerase II (RNAPII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Binds to RNA polymerase II.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.-" evidence="7"/>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EHS63031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890035.1">
        <property type="nucleotide sequence ID" value="XM_003889986.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QR56"/>
    <dbReference type="FunCoup" id="H6QR56">
        <property type="interactions" value="571"/>
    </dbReference>
    <dbReference type="STRING" id="418459.H6QR56"/>
    <dbReference type="GeneID" id="13540935"/>
    <dbReference type="KEGG" id="pgr:PGTG_21323"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21323"/>
    <dbReference type="eggNOG" id="KOG1532">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037460_1_3_1"/>
    <dbReference type="InParanoid" id="H6QR56"/>
    <dbReference type="OMA" id="MIIVFNK"/>
    <dbReference type="OrthoDB" id="243313at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17870">
        <property type="entry name" value="GPN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000579">
        <property type="entry name" value="GPN-loop GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030230">
        <property type="entry name" value="Gpn1/Npa3/XAB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF8">
        <property type="entry name" value="GPN-LOOP GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="327"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="295"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="337"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="358"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="9">
        <location>
            <begin position="387"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS63031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="398" mass="44307" checksum="25E860CA4B712085" modified="2012-04-18" version="1">MSSPTQTATNQSKGKMKSEQEGADNDKNQPPPVCIICIGMAGSGKTTFVQRLNSYLHSIKKPPYILNLDPAVSSLPFQPNIDIRDTVNYKQVMKQYNLGPNGGILTSLNLFTTKFDQVLSYVEKRSSSTDFVLIDTPGQIEIFTWSASGAIITDAIASSLPTVVAYIIDTPRTTAPATFMSNMLYACSILYKTRLPFLLVFNKTDVQAHDFALEWMEDFESFQQALVAQNQSNESEGGSGYMSSLMNSMSLVLDEFYKNLRAVGCSAMTGFGMKEFFEAVDEARQEYENDYRPELVRRQEERERLKEEKKNESLEKLVKDLKLSSDSSAMAASKAKKNTEAKDPTELRADELSEWEEADGRTLDKQDVSEAAMPEPTKRLDSMGLESDGGGGQWPAPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSK3</accession>
    <name>H6QSK3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21846</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178295">
        <property type="protein sequence ID" value="EHS63754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889513.1">
        <property type="nucleotide sequence ID" value="XM_003889464.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSK3"/>
    <dbReference type="STRING" id="418459.H6QSK3"/>
    <dbReference type="GeneID" id="13541881"/>
    <dbReference type="KEGG" id="pgr:PGTG_21846"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21846"/>
    <dbReference type="HOGENOM" id="CLU_2373829_0_0_1"/>
    <dbReference type="InParanoid" id="H6QSK3"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="95" mass="10369" checksum="7D2AE089B8BCC09A" modified="2012-04-18" version="1">MTSAGMATMMGGAQIGFGTTTMGIARYPKARARSRDYLRHSNLTHRSLQEINYLTSNPVLNPLSQQSALIPGIKWPHKILIKTKAAQQGGPNHPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSP5</accession>
    <name>H6QSP5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21745</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
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                <person name="Kohler A."/>
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                <person name="Lucas S.M."/>
                <person name="Mago R."/>
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                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
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                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
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                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
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                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
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                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
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            <end position="66"/>
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    <evidence type="ECO:0000256" key="1">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="EHS64424.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
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    <sequence length="93" mass="9994" checksum="60709E678227B892" modified="2012-04-18" version="1">MLRLRDTALVRSSKYYHRVGNRKSGRRSRQISPPKYGGSYPVVGSSAPGGQAPAVTPGASDTQMETRPATFSEDITDVVKVVGIPLQAYGKKS</sequence>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
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                <person name="Cuomo C.A."/>
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                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
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                <person name="Murat C."/>
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                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
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                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
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                <person name="Tuskan G.A."/>
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                <person name="Ellis J.G."/>
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                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <source>
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        </source>
    </evidence>
    <sequence length="713" mass="79391" checksum="2BED39BEA1826C40" modified="2012-04-18" version="1">MADAFLSSVSRELTEDRGHRSSHRSRDDDRPRRRSRSPHREERRRRDDDPERTAERGSRYAERSSRYDERDRDRSERDHGRDRDRDRDRDRDRDRRSSRYDDRDRERDRDRGRDRDRERDRDRGRDRDRDRDRDRDRYARRDDVSNGGGRRRGGHGSFGGGGGGGFGNGGGADGGVFGSPNIRRSPTPEGTIPISKRPRKYTAWDVKPPGYANVSAMEAKISGSFMLPGQTRPASQTPGGPVNGGNGTAPANMSMHNSNFPGHPHLNPNHHWVNPNQQNAHGHSQMTAQHPNQFQMNSLGRDGGFGMPGSLPPIHFKPAQGQSGPVAQTQSFARQARRLYVGNILHTANEMNVAEFFNAKMKELGLLARNNEDGMAISISENPVVAVQVNHEKNYAFVEFRNAEEATHGMSFDGIIFQNQALKIRRPKDYTGPDHAGPTHIPGVVSTNVPDSPNKIFIGGLPSYLTDDQVMELLKSFGELKSFNLVKDTSSGGHVSKGFAFCEYVDPDLTDIACQGLNGMELGDRYLVVQRAQIGQNAKKEKENNPDGQRNNYNQFNNFAGGQATAAASSVLAAVKSGEGEKTRVLQMLNMVNQEELVDDQEYGEILEDIRDECGKYGKIEGVRIPRPIKNEKGRIDLKASESVDGLGKVFVMFEKVDECAAALLAIAGRQFAGRVIICAYAPEDAMTEGSKPEQPPEQEERPTEDTPAEEGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUJ3</accession>
    <name>H6QUJ3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22341</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178343">
        <property type="protein sequence ID" value="EHS64705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888822.1">
        <property type="nucleotide sequence ID" value="XM_003888773.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUJ3"/>
    <dbReference type="STRING" id="418459.H6QUJ3"/>
    <dbReference type="GeneID" id="13541567"/>
    <dbReference type="KEGG" id="pgr:PGTG_22341"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22341"/>
    <dbReference type="HOGENOM" id="CLU_008138_1_1_1"/>
    <dbReference type="InParanoid" id="H6QUJ3"/>
    <dbReference type="OrthoDB" id="10277699at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266">
        <property type="entry name" value="CHROMOSOME 15, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266:SF1">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="757"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="757"/>
            <end position="770"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="852" mass="95158" checksum="3A79FFDA4BB83AD4" modified="2012-04-18" version="1">MIFQARARCPSIFKSSWPRSAITTTSILSGHRRNPHNTPLLSTSHWLLSTANASMHKDLQTPPLESHSNYRLQERAEKAQDSDGFLGQNGTTGGSTKIDRDGRIAPSSHEEAERLFNHPLEDGIVAEAFNNHEYLDTKRIVDPTLQTLQKMAREWTPDKYLAPSTSLVATSTGGKSRLLKELSRRTCVVYICLRPKDATGYPPRSEYPAGVLLDSKCPTLQTRYEELLCAILHTVADYFSAQEPGSRQERLERWMLHSFPHANRSGDPPFWTEVATKMNEISSRSARLTAYEKDAQLWEALNRMKESTQFIGQDDLRVLLAIDEASELFASYSSSPDLSFLSIFQGALQRIPSHSSEFFSILADTKSLLSTFHPTSPYYINHPYYRIGKHKHKKLFDPIYEIPTFDLHVTDPPTDQQQLQSAFRLLSYGSPFWRAYANEAKRKGLADDEIIQGLTQYTLQKLLSTSGQSVPAGSLSDSQVFGLLGSTIQPSTLGASYLNAKLISSHGAQCVHLDPSQLELISGYPSQFILSSAANQYLASDEARLIRCIQVLASINRQRLFGLSDVDRLVSRIILLRAMQITIQNTPPPPPPPVVGPDSERLITMPFGHSVRLVDFLQTFTGWDQEHLELGSIDKENKERLLAEAHLFWNHFISIDHTPSSAGLLRQLYRGSAVTCRHHQPSFDQLFTIYLRSESGPTTALIGEKQITFGAVCVSNSSDDESVAQASYQWTPAHAGIKPQEHRHPYLVLNFRLNEASSSTKPPNSPSASSPHIVPIPNGKASKNQARRRAGLAFYGLKAFPFLNQDLREALQELLDSEASFRSLISDNSSDLYRKFVLKTSPGVYPSSSSSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUP6</accession>
    <name>H6QUP6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22477</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178348">
        <property type="protein sequence ID" value="EHS64758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888766.1">
        <property type="nucleotide sequence ID" value="XM_003888717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUP6"/>
    <dbReference type="STRING" id="418459.H6QUP6"/>
    <dbReference type="GeneID" id="13541634"/>
    <dbReference type="KEGG" id="pgr:PGTG_22477"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22477"/>
    <dbReference type="HOGENOM" id="CLU_004591_1_0_1"/>
    <dbReference type="InParanoid" id="H6QUP6"/>
    <dbReference type="OrthoDB" id="2506469at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="135"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1132"/>
            <end position="1160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1140"/>
            <end position="1160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1160" mass="129817" checksum="1AA7C0AB44C63E29" modified="2012-04-18" version="1">MERGELDWALEGWGRRDQWADSRQTGQSLGCGQSGFRLIPPSVGPTVPQLVIELDQVPLLLRGSDFPRIMPLLKYFNCIATADGPSYVCLICNNARPTKDYRRHSRRYNHQVNIARHEEAELIRRHDDVNYDNIDVQPDILDSPEGLQGRARTAPASAEGGVATVGPSLPGGFDLSSGPSGENSGGLRDTAVPVEEEPALPEDLHNDDDGLYDLLNAEGSLSDNGDGTPGNSGNRGGATAAEPMAQADWFPFRNKMIRGILRLCDMQIPDWGTVRDSRARIRDITQSTDLGNPLVSPHLEFYPDNTEGQNVSRFSQSLKWLQYMAPEDRAQMCDVNDKHFYIFEPVQLLTKEVVVPIFLYMYHWRLHAKCIEIGADHVTELGEQTRITIPSSLAFDDHRLTAWDIREFDLTYSEIRMGDGILLTEACGGSIYDSDGLVDREIPLPNHWRTVADGKVIRNVPVTLYCDDTSGNTSKQFNKHVSFYFTLSGLPPHVSNQEFNCHFLSTSNIGSALEIAEQVVDEFNEMGTKGYGAYDASIGQSVWVMSTVLCFLADSPMHAEITNTPNPGQSLNPCRVCTLSCEKKSMKTSREYVQRFLGLDNLGILVPNAMRSWDGTRMETKRLFSVARSVNMTQFKKQCLASGIKDAINIRLLTDAKTSDRVNTTMEELQNAQSNRLYNAFLKLHGFDGVLDTPVEVLHVVLLGIVKYLARDFIKSLSETVKLELIARLRSFNCNALNIDSLKPNYLIRHILSIVGRDFKVILQCAPFVFFDYMTEEKRKIWSALCRLSPFIFQTSIADMGEYQIQLKLHIDIFLYHLISSTAQWVNKPKIHMLRHLPEAILRFGPASLCSTEKFESYNGVLRKASVHSNKLAPGRDIALTFNSYACIKFWLSGGYLIDRSTGLLTRASSQVRQAFDENPNLQESLGYNASATFLPGRDEFPACHAGHYRLQSTFNPPASLVDLAATLCRQRERLILRRKRGSPAVRGLNGSCMVGEVDSIWEVDWGYAITYHLDVVGFDVDGYDEHYDMTRIARTGYIHLVNATDVAGAINVQHNCHGGGCQLDRTGSTFRERILTEDKCDEVQHSDIDHFLINAASLHNPGLHQQMSLPAALPISPSDWRDAISLGLEVWGHPNEPGLDSDDSDDMSEATQEEEEDEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>H6QVA6</accession>
    <name>H6QVA6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22689</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178389">
        <property type="protein sequence ID" value="EHS62807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888561.1">
        <property type="nucleotide sequence ID" value="XM_003888512.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QVA6"/>
    <dbReference type="GeneID" id="13540788"/>
    <dbReference type="KEGG" id="pgr:PGTG_22689"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22689"/>
    <dbReference type="HOGENOM" id="CLU_2777160_0_0_1"/>
    <dbReference type="InParanoid" id="H6QVA6"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="69" mass="8049" checksum="E2C2EFC0460F87A8" modified="2012-04-18" version="1">MGTQTQAPPDGFDRVSGERQKSRPRLFRFGYRIRLFCWLLFLSTERQSTGGTRRRSAPSVNNEHPRQPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRY4</accession>
    <name>E3JRY4_PUCGT</name>
    <protein>
        <submittedName>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
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        </citation>
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            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
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                <person name="Selles B."/>
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                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
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                <person name="Ellis J.G."/>
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                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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            <dbReference type="PubMed" id="21536894"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
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                <person name="Nusbaum C."/>
                <person name="Devon K."/>
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                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
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                <person name="LaButti K."/>
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                <person name="DeCaprio D."/>
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                <person name="Koehrsen M."/>
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                <person name="Pearson M."/>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
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                <person name="Pearson M."/>
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                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
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                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
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                <person name="Nusbaum C."/>
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                <person name="Alvarez P."/>
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                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
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                <person name="Montgomery P."/>
                <person name="Pearson M."/>
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                <person name="Szabo L."/>
                <person name="Dean R."/>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
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                <person name="Pearson M."/>
                <person name="Quesneville H."/>
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                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
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                <person name="Tuskan G.A."/>
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                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JSG6"/>
    <dbReference type="STRING" id="418459.E3JSG6"/>
    <dbReference type="GeneID" id="10547141"/>
    <dbReference type="KEGG" id="pgr:PGTG_01532"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01532"/>
    <dbReference type="HOGENOM" id="CLU_1741438_0_0_1"/>
    <dbReference type="InParanoid" id="E3JSG6"/>
    <dbReference type="OrthoDB" id="25002at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20546">
        <property type="entry name" value="CYCLIN_SpCG1C_ScCTK2-like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP74939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="175" mass="19496" checksum="A6D05FBE12FA4495" modified="2011-01-11" version="1">MVDKPNGEAEGALEIKLKELPSNVNVGPINNHPTQQEFQVLKQEILYYEDILLRTLCFDLACQVHPRVPHLGPAFEIVDCSQNGRSGQSQVDHQAAWGFINDSLMSPLCLVAKPELIAASAFLLAISHHLSKSPPSYPDQEGNHREPHNIEKNPVDFHCFLNHPPRDGPEEGLIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUW1</accession>
    <name>E3JUW1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01167</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75836.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320255.2">
        <property type="nucleotide sequence ID" value="XM_003320207.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JUW1"/>
    <dbReference type="GeneID" id="10532751"/>
    <dbReference type="KEGG" id="pgr:PGTG_01167"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01167"/>
    <dbReference type="HOGENOM" id="CLU_035530_0_0_1"/>
    <dbReference type="InParanoid" id="E3JUW1"/>
    <dbReference type="OrthoDB" id="10282893at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="255"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="303"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="68"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="269"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="308"/>
            <end position="319"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75836.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="656" mass="76616" checksum="B5A170147D8327A3" modified="2012-04-18" version="2">MLKMVHQVYQFYIILARIGATSAAFNNLIHDPFAQLLEDRAQHDVLGDVEHFATGLLDHQKDAAQHLSSSSGPRSFSHPSVPSPEFNFQTTWPTFPEADEKLWPWLNEFDQLLPSMNIFPKPPEYSTQDTRYPSNYDRQISNTESHGQIRGLFREGASQSIRHHENPTEKFQGSAKKPQLPSQEVQRPSKKSWASRLESEKESKKRKLGDFDDPNKLNKNPGLQIKTTDDRATRDIFPTWNFDWDAFFDSKDPARGLEEHPSRESQDYSSAVSSLPSNSNNPSIENNPFKTLTDSSVVVIEDPHKSNGRSQNQIESQFSGGKPAKFGKQSDQSIVKSQNLIYQSPKFDERTKEEFEISRRAAIKFSQKSEKRKLFNFEDPEWRKEMTKKIMNEVPVSGQNDQLPRLSFDRNAFFLESQSGKSTIQLHALSPMFRTMKIHEGQKMSIPEAEVTLFLRRSYNPRYYKDDEYQAWKEKVQIFLQHQELWAKYWNQLKMESCFKEIGHKKLKEIFPLYLFYISMINSIIPRAVGQELSEEDELKSAALTFINYAQGIEKYVNNRHTAQIKWTLMEEKRLDYFQRLLREDTKGSRVRINLWAFLDVWMENNRPLLFSTNQTLTGMFTQKGRKFFNQIFISSIENLIKYYEKLNAESLLKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUX2</accession>
    <name>E3JUX2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01178</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320266.1">
        <property type="nucleotide sequence ID" value="XM_003320218.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JUX2"/>
    <dbReference type="GeneID" id="10532762"/>
    <dbReference type="KEGG" id="pgr:PGTG_01178"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01178"/>
    <dbReference type="HOGENOM" id="CLU_776434_0_0_1"/>
    <dbReference type="InParanoid" id="E3JUX2"/>
    <dbReference type="OrthoDB" id="2504023at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="312"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="357" mass="38481" checksum="E689AB22F81742EC" modified="2011-01-11" version="1">MYMDPYTASGNPAIDPSLPRHSSGSPNLVDSNDKQIQNQPELAEDENEEESNFGEIGMFGDFCLMCGEMIDGEVFDGSAYCSEECEYHQRITSESDWCDLSLEGYQLPSYGCSTAVSSARTSPLNSPNLQASGRLAILCSGPPPCELLLPLDLGERLLGAKAVAERYLENRPKSWQEEGEETEEGKMKKKKKEDVEERISGADEGVEEAGDRKRAGAGQSSSSLHSSTITPSQKPLVSTGSHLKSRPALAQLSDLEDFWGPPSKQTASTSRPASPHSLASIDPPLPSSSTVNPNSGQTLSSSRRRVSEPVFSHSQPIEVSPSNPHLYHSSFSLTSLSSLSPSSSQECAQSCCSTHFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3JV37</accession>
    <name>E3JV37_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01243</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75912.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320331.2">
        <property type="nucleotide sequence ID" value="XM_003320283.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JV37"/>
    <dbReference type="GeneID" id="10532990"/>
    <dbReference type="KEGG" id="pgr:PGTG_01243"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01243"/>
    <dbReference type="HOGENOM" id="CLU_2062636_0_0_1"/>
    <dbReference type="InParanoid" id="E3JV37"/>
    <dbReference type="OrthoDB" id="10301592at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172794" description="Secreted protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75912.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="13490" checksum="5F96AC03FDC31296" modified="2012-04-18" version="2">MMCSMRFLVLIAIMLVGQVVQAAQTDDVFRCQGDKPQGVCLGTRIKDVKKRVDYWVISARGVTGGFSCDPKRKEYAGGFHNVCCKKELDLKKFTKVSFYPTCMFTILNFHHLESKLMKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXJ1</accession>
    <name>E3JXJ1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Phosphatidylserine synthase 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGTG_02227</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12">Phospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76766.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321185.2">
        <property type="nucleotide sequence ID" value="XM_003321137.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXJ1"/>
    <dbReference type="STRING" id="418459.E3JXJ1"/>
    <dbReference type="GeneID" id="10528788"/>
    <dbReference type="KEGG" id="pgr:PGTG_02227"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02227"/>
    <dbReference type="eggNOG" id="KOG2735">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037661_4_0_1"/>
    <dbReference type="InParanoid" id="E3JXJ1"/>
    <dbReference type="OrthoDB" id="10265393at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106245">
        <property type="term" value="F:L-serine-phosphatidylethanolamine phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006659">
        <property type="term" value="P:phosphatidylserine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004277">
        <property type="entry name" value="PSS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15362:SF7">
        <property type="entry name" value="PHOSPHATIDYLSERINE SYNTHASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03034">
        <property type="entry name" value="PSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="9 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="153"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="183"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="372"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="403"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="440"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="473"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="50"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EFP76766.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="511" mass="59707" checksum="17E3B2653B647E84" modified="2012-04-18" version="2">MTVKTRRQLINDRTSKLEQEQEQEQLPKQSIPLRTTSSQLTTTTTTTTDQEQEQEQDQEQDEEPDDFLFSDDPAHPCSQTHLNNRANYSSASRNNPFFFGQPDDKLEPYQMKHDDSVKWVYRPSTLTAAAIGTSVLASLAYNSHIFPRSFRSHLGVISGVTVFLVFCMLNFRDGPFRRPHPAFWRIVLGLNLLYFMILSFLYWTDKSSARELIRYFDPSLGVPLPEKSYAENCEFTPHNVWQGIDIFCLAHSLGWFAKAVVLRDHWFCWILSVGFEVMEYSLQHHLPNFAECWWDHWILDVLICNWGGLWLGMKTCEWLRVSPFSWRGLRPVVVSENLTTSRRRAAKYAAQQFTPHDWTEFRWEGTKRFRNYISTVALLTVFLLSELNVFYLKSLLWLAPEHPIVIIRLAAMFLWALPAVREYYDYATYTKRVKRMGSHAWLSMATVILELLIVIKWSKGEFEESVMPIKVKIFWSITLILLIVYPAIKFGLPALKQHQHHSSSKKSSRTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXZ2</accession>
    <name>E3JXZ2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02378</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321336.1">
        <property type="nucleotide sequence ID" value="XM_003321288.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXZ2"/>
    <dbReference type="GeneID" id="10529102"/>
    <dbReference type="KEGG" id="pgr:PGTG_02378"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02378"/>
    <dbReference type="HOGENOM" id="CLU_034223_0_0_1"/>
    <dbReference type="InParanoid" id="E3JXZ2"/>
    <dbReference type="OrthoDB" id="2499220at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="379"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="414"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="90"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="443" mass="48630" checksum="81A8DA0E5E01E5EB" modified="2011-01-11" version="1">MASSPTQEAHSLSTFHWLFSEEMPDESLPQFFPRVLDFEQSSTQPSHGTPFRPGPRYRRDQSYQEDSSPIYDPDCSPCYYPDSPQWAPGPISQSISSPQWVPGPTSQSISSPQWAPGPSSGSISRPTLQLSQAPELPFECQGRKRHTESPQEADQRPRKYARPSDSRSDSHVDSEKPDGSAAGTPPTTATHSLHSSESPSSSSLACFDHIKEIQRLRAQLVISESRNARAMADLAEARLCVDFLMNEVGRVMGSTSQAHPAAERVRQIISSNTELRVPLFTRASPEMTTLADSQQDALPVVDNADAAWSEDLDPRNPNSYYFSEIPMAGPSTRPGSISLAESSQLAPIGGFQGYGSMTSVGGEGSTSYSEMVREASLDYRSTSSLTEPGRSRDPSGSQLVPDWSHTRVDSYYFSEPLPPFDSAQETTRESYAPNYELPATRYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3K021</accession>
    <name>E3K021_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03602</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77646.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322065.2">
        <property type="nucleotide sequence ID" value="XM_003322017.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K021"/>
    <dbReference type="GeneID" id="10538854"/>
    <dbReference type="KEGG" id="pgr:PGTG_03602"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03602"/>
    <dbReference type="HOGENOM" id="CLU_1826264_0_0_1"/>
    <dbReference type="InParanoid" id="E3K021"/>
    <dbReference type="OrthoDB" id="10477702at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77646.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="141" mass="15447" checksum="9467BA579CD28FE7" modified="2012-04-18" version="2">MAVTSARKAAQEADSESRPEETSRSLVNDNVQPRRAARSRAQAPIATLEHWNVPLPANSDPAKKKLPFLTSEAKQSTSDDLNPSPKGLHPDSPTQLGTFATTRQLFHTGYYFSRGIKPERFMVISAPSITTPDESNWIGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3K053</accession>
    <name>E3K053_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_03634</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77678.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322097.2">
        <property type="nucleotide sequence ID" value="XM_003322049.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K053"/>
    <dbReference type="GeneID" id="10539050"/>
    <dbReference type="KEGG" id="pgr:PGTG_03634"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03634"/>
    <dbReference type="HOGENOM" id="CLU_039964_0_0_1"/>
    <dbReference type="InParanoid" id="E3K053"/>
    <dbReference type="OrthoDB" id="2496461at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP77678.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="323" mass="36797" checksum="74FA69FCDABA4B3C" modified="2012-04-18" version="2">MSHFGIDLFGRENPPPGYHSALELWRIQNLGPPPDQLATQMIDLSTSFKLVYPDKNSNNKLTWIPRKSKRKFLISIPAVSTTFEQFHEIVARKCEENSEGAGVIIQNALESGSPGINWKVWMNLPAAHEFKKNANYKVNDINSFTHWTATIIANGKDRTDASLEVEMISPADLEKEAKAAVKIKQHVMTHAAAQRASTSKHQDPSTLVAAKDFDIYNVYKDQIFDAHPPNIKYHKRIPVFIDPADETRYILLTTVNVEKWAKALMEPNTTVSLHSPPPELKFEKLSASKKRKLDCCSSSRTRRDQFPIRFRWVFRSRSRSQLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3K227</accession>
    <name>E3K227_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Survival protein SurE-like phosphatase/nucleotidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04352</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322815.1">
        <property type="nucleotide sequence ID" value="XM_003322767.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K227"/>
    <dbReference type="GeneID" id="10535994"/>
    <dbReference type="KEGG" id="pgr:PGTG_04352"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04352"/>
    <dbReference type="HOGENOM" id="CLU_1190384_0_0_1"/>
    <dbReference type="InParanoid" id="E3K227"/>
    <dbReference type="OrthoDB" id="4018688at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1210.10">
        <property type="entry name" value="Survival protein SurE-like phosphatase/nucleotidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002828">
        <property type="entry name" value="SurE-like_Pase/nucleotidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036523">
        <property type="entry name" value="SurE-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01975">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64167">
        <property type="entry name" value="SurE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Survival protein SurE-like phosphatase/nucleotidase" evidence="1">
        <location>
            <begin position="53"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="233" mass="25735" checksum="633A848633784D54" modified="2011-01-11" version="1">MIGIPILRPILGSGKCAKELESQNDEAIKGLSAKFRLLEEITVYRRLENKRRENTGLENQFSGTLGAASLGLRRGVAAITIPVDDENWHSYQSGYGKKSSEIYVNAMMRVIEELLKVEKTEGPNYLPAGCVLNINLQQAGPQTNCNRAQDYRFVLTTIFGSRHQCGIDLCGSHFLPSEQAVLDSQNGCWVPLIQSHSDPGAMSLLVCKSCGGLMNNEIVEFIVDRLKHIVVVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>E3K357</accession>
    <name>E3K357_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP synthase subunit beta</fullName>
            <ecNumber evidence="14">7.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGTG_04870</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="14">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="14"/>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323333.1">
        <property type="nucleotide sequence ID" value="XM_003323285.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K357"/>
    <dbReference type="FunCoup" id="E3K357">
        <property type="interactions" value="210"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K357"/>
    <dbReference type="GeneID" id="10537102"/>
    <dbReference type="KEGG" id="pgr:PGTG_04870"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04870"/>
    <dbReference type="eggNOG" id="KOG1350">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022398_0_2_1"/>
    <dbReference type="InParanoid" id="E3K357"/>
    <dbReference type="OMA" id="SMEEGGW"/>
    <dbReference type="OrthoDB" id="14523at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042776">
        <property type="term" value="P:proton motive force-driven mitochondrial ATP synthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18110">
        <property type="entry name" value="ATP-synt_F1_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18115">
        <property type="entry name" value="ATP-synt_F1_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01133">
        <property type="entry name" value="F1-ATPase_beta_CD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1140.10:FF:000001">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.170:FF:000004">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000026">
        <property type="entry name" value="ATP synthase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="entry name" value="Bovine Mitochondrial F1-atpase, Atp Synthase Beta Chain, Chain D, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01347">
        <property type="entry name" value="ATP_synth_beta_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005722">
        <property type="entry name" value="ATP_synth_F1_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050053">
        <property type="entry name" value="ATPase_alpha/beta_chains"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01039">
        <property type="entry name" value="atpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15184">
        <property type="entry name" value="ATP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15184:SF71">
        <property type="entry name" value="ATP SYNTHASE SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039072">
        <property type="entry name" value="ATPase_subunit_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 14" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="7 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 14" id="KW-0139">CF(1)</keyword>
    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-1278">Translocase</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="15">
        <location>
            <begin position="210"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EFP78914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="542" mass="58393" checksum="48560B8DAF706892" modified="2011-01-11" version="1">MHRGSGLMRAIPRSATKARRANSALLNATRTLVSAAKPAVPAVVAKSSLPRFTKNQVDQPRSYASEAKAQIGQIKTVIGAVVDVQFDSENLPPILNALEVQDFGQRLVLEVAQHLGENTVRTIAMDGTEGLVRGQKVVDTGAPIQIPVGPECLGRIMNVIGEPIDERGPIKTKTMMPIHAEPPEFIDQSTIPEVLVTGIKVVDLLAPYARGGKIGLLGGAGVGKTVFIQELINNVAKAHGGYSVFCGVGERTREGNDLYHEMIETGVIQLNNDKSKAALVFGQMNEPPGARARVALTGLTLAEYFRDEEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLSTDMGGMQERITTTRKGSITSVQAVYVPADDLTDPAPATTFAHLDATTVLSRGISELGIYPAVDPLDSKSRLLDPRIVGPEHYNLATAVQKLLQDYKGLQDIIAILGMDELSEDDKLTVERARKVQRFMSQPFAVAEVFTGLEGRLVPLKDTIESFKGILAGQHDHLPESAFYMVGAIDDVKAKADKLAAEMKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3D8</accession>
    <name>E3K3D8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PAN2-PAN3 deadenylation complex subunit PAN3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">PAB1P-dependent poly(A)-specific ribonuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Poly(A)-nuclease deadenylation complex subunit 3</fullName>
            <shortName evidence="7">PAN deadenylation complex subunit 3</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">PAN3</name>
        <name evidence="10" type="ORF">PGTG_04951</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein PAB1. PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. May also be involved in post-transcriptional maturation of mRNA poly(A) tails. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with RNA and with PAB1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer. Forms a heterotrimer with a catalytic subunit PAN2 to form the poly(A)-nuclease (PAN) deadenylation complex. Interacts (via PAM-2 motif) with poly(A)-binding protein PAB1 (via PABC domain), conferring substrate specificity of the enzyme complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="7">Contains a pseudokinase domain. The protein kinase domain is predicted to be catalytically inactive because some of the residues important for catalytic activity are substituted and it lacks the equivalent of the binding site for a peptide substrate. However, it has retained an ATP-binding site and ATP-binding is required for mRNA degradation, stimulating the activity of the PAN2 nuclease in vitro. The nucleotide-binding site is juxtaposed to the RNase active site of PAN2 in the complex and may actually bind nucleosides of a poly(A) RNA rather than ATP, feeding the poly(A)-tail to the active site of the deadenylase and thus increasing the efficiency with which this distributive enzyme degrades oligo(A) RNAs.</text>
    </comment>
    <comment type="domain">
        <text evidence="7">The N-terminal zinc finger binds to poly(A) RNA.</text>
    </comment>
    <comment type="domain">
        <text evidence="7">The pseudokinase domain, the coiled-coil (CC), and C-terminal knob domain (CK) form a structural unit (PKC) that forms an extensive high-affinity interaction surface for PAN2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. PAN3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78995.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323414.2">
        <property type="nucleotide sequence ID" value="XM_003323366.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3D8"/>
    <dbReference type="FunCoup" id="E3K3D8">
        <property type="interactions" value="123"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K3D8"/>
    <dbReference type="GeneID" id="10530816"/>
    <dbReference type="KEGG" id="pgr:PGTG_04951"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04951"/>
    <dbReference type="eggNOG" id="KOG3741">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016423_1_0_1"/>
    <dbReference type="InParanoid" id="E3K3D8"/>
    <dbReference type="OrthoDB" id="204958at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031251">
        <property type="term" value="C:PAN complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.3700:FF:000001">
        <property type="entry name" value="PAN2-PAN3 deadenylation complex subunit PAN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.5160:FF:000002">
        <property type="entry name" value="PAN2-PAN3 deadenylation complex subunit PAN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3700">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.5160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03181">
        <property type="entry name" value="PAN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030844">
        <property type="entry name" value="PAN3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041332">
        <property type="entry name" value="Pan3_CK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12272">
        <property type="entry name" value="DEADENYLATION COMPLEX SUBUNIT PAN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12272:SF11">
        <property type="entry name" value="PAN2-PAN3 DEADENYLATION COMPLEX SUBUNIT PAN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18101">
        <property type="entry name" value="Pan3_CK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pan3 C-terminal knob" evidence="9">
        <location>
            <begin position="602"/>
            <end position="739"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="142"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="437"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="region of interest" description="Knob domain" evidence="7">
        <location>
            <begin position="650"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="611"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="30"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="146"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="447"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="415"/>
            <end position="422"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="507"/>
            <end position="508"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP78995.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="741" mass="81719" checksum="410B3A514B6B46BE" modified="2012-04-18" version="2">MGTRSAAVQIRPPSSTTSATSSPRPVLASISHSAVPASNLQQKVSDADNQSHNESNGSSQFNHQTSPTISTGRKSSPSSPRKFSLPHPARPATATPIASSGASLTTNAPVFVPSGTLNPYHPPSGRTGQQVKLNKSAAEFAPSGLVPTSDSSTLYNHSNTSESAPSYSSSSSTSYPGEPTFHPHNPYAYDAERLLHSQATHHPITEAYMPHDGFMPHNRVSAPSLSNFQRPLNYHLYAPPLPHSSNQHPQQLALNKFFMPASIRETLQKKSAATLQGPTHEVARTPEDLGVYHSLVVLDSPHVTNSSTSSLHPTITGGATNGSSTMNPAANAHRSPYSSRVLKNPCWIYKAFCSLDGKAYCLYRVEGVRLKQGQGEAAIGQVERWRKIRHPAIVNVREAFTTRAFGDHSILFAYDYHPLSQTLYDEHISAKKSSTRTFATDEVEHPSNLSNPTQSGSRYNRHRSANGHPSRIGLSEERIIWSYVIQIANAIKTVHSANLSVRTIDPTKIILTGTNRVRINACGILDVTEYSPNQSPEDLQVEDLRDLGCLICSLASSSICIKTDNNSLTKGVEYVKQFYSAELSEVVAYLLAPALDGHTISIEGLLRMLWSRSLDEMTKVFNHNDLLEESLSRELENGRLVRLMVKLGFINERPEFDHDPNWSETSERYLLKLFRDYVFHQVDSQAKPVTDLSHVLMCLNKLDTSSDEKLTLISRDDQTCAIVTYAEIRRIMDSAFRDLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3M4</accession>
    <name>E3K3M4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP79081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323500.1">
        <property type="nucleotide sequence ID" value="XM_003323452.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3M4"/>
    <dbReference type="GeneID" id="10530902"/>
    <dbReference type="KEGG" id="pgr:PGTG_05037"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05037"/>
    <dbReference type="HOGENOM" id="CLU_619842_0_0_1"/>
    <dbReference type="InParanoid" id="E3K3M4"/>
    <dbReference type="OrthoDB" id="2500581at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013248">
        <property type="entry name" value="Psh3/Shr3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28228">
        <property type="entry name" value="SECRETORY COMPONENT PROTEIN SHR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28228:SF1">
        <property type="entry name" value="SECRETORY COMPONENT PROTEIN SHR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="435" mass="49245" checksum="87DA260E3F36810E" modified="2011-01-11" version="1">MSAQTIENQSSINKSILNNLVHGDDTLDIKQHSSLTPRRENCVYGLYSKYQEFDPTSAPSQTLWSHIISEAAHQPTAHESPKTSDNFIQFSAPFLCDQYLVKLKKLFEELAESKAGWSYTFRHHNAVILEGILEDLQELKNGTGAENTKYRNTAQIHFPDTSNHQPKIRSSSHSDENDHSGSADGDDPPPPVLPTKSENDFENATLITIAMSPFKTPPKNTRGCNLKISKRSHLFRQHYLIRSISNDLYLYDHHQNQMGTTKIFHGTRQSSLLSFNENGIQPAFGRNEFSYAPAFYASNDPQQAFEHPLHNHLGAGTNNTIDLIAVLQFEVAISILHGNGSPGNEQPPFNVYRFSKYSQTNLIHWQKFCDRNMKARISENHDYVLVIGPICLPSKDNKPVTCLQMKNSKIPTTQIAFCSRRSRAWIGRQIKEHIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3U1</accession>
    <name>E3K3U1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Oxysterol binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_04699</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323162.1">
        <property type="nucleotide sequence ID" value="XM_003323114.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3U1"/>
    <dbReference type="FunCoup" id="E3K3U1">
        <property type="interactions" value="71"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K3U1"/>
    <dbReference type="GeneID" id="10536680"/>
    <dbReference type="KEGG" id="pgr:PGTG_04699"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04699"/>
    <dbReference type="HOGENOM" id="CLU_012334_0_0_1"/>
    <dbReference type="InParanoid" id="E3K3U1"/>
    <dbReference type="OMA" id="WHTRPKG"/>
    <dbReference type="OrthoDB" id="14833at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008142">
        <property type="term" value="F:oxysterol binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120009">
        <property type="term" value="P:intermembrane lipid transfer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.160.120:FF:000010">
        <property type="entry name" value="Oxysterol-binding protein homolog 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3490:FF:000018">
        <property type="entry name" value="Unplaced genomic scaffold supercont1.15, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.2720">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.160.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037239">
        <property type="entry name" value="OSBP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000648">
        <property type="entry name" value="Oxysterol-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018494">
        <property type="entry name" value="Oxysterol-bd_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972:SF184">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN HOMOLOG 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10972">
        <property type="entry name" value="OXYSTEROL-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01237">
        <property type="entry name" value="Oxysterol_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144000">
        <property type="entry name" value="Oxysterol-binding protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01013">
        <property type="entry name" value="OSBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP78743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="408" mass="46027" checksum="D9E5461EB69D8D94" modified="2011-01-11" version="1">MATEEEAVVPTNQRSSWTSFLKSIATFSGDLSSLTAPSFILSPTSLCEFPSYWGEPHREFAAISTGKNPEERMVLTLRWFISTLKGQFTRRETQTGTEKKPLNPILGELFLGSWNSGPSTDENSTGELRLWTEQVSHHPPVSAYCLINAKAGVSYQGHCGQKTSFSGKSVIVKQVGHGLLRIKLPDNTIESYLITLPKLRIEGLWMASPYIELTENSHIQSSSGYHTVLEFKGKGYFSGKAHSFTAKIYSQPKITSGVSPVYEIEGQWHGLSKFVKQVPKWWQGSGEKFLDQTEEKLDEITVQDISKQGPLESRRVWKDVADGIRSGDFEAASSAKSRLENEQRAKRKEELANGTPWQMSHFDRVDSDEEYARLAEMCGHSPPTEEAYYFKFENEYIQETLSKQQQQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>E3K7J6</accession>
    <name>E3K7J6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_06443</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324906.1">
        <property type="nucleotide sequence ID" value="XM_003324858.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K7J6"/>
    <dbReference type="FunCoup" id="E3K7J6">
        <property type="interactions" value="532"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K7J6"/>
    <dbReference type="GeneID" id="10539498"/>
    <dbReference type="KEGG" id="pgr:PGTG_06443"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06443"/>
    <dbReference type="HOGENOM" id="CLU_003008_0_0_1"/>
    <dbReference type="InParanoid" id="E3K7J6"/>
    <dbReference type="OMA" id="CFLAGEF"/>
    <dbReference type="OrthoDB" id="343875at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016593">
        <property type="term" value="C:Cdc73/Paf1 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000993">
        <property type="term" value="F:RNA polymerase II complex binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001911">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:001949">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031101">
        <property type="entry name" value="Ctr9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14027">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN CTR9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14027:SF2">
        <property type="entry name" value="RNA POLYMERASE-ASSOCIATED PROTEIN CTR9 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13174">
        <property type="entry name" value="TPR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="11"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="981"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="930"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="995"/>
            <end position="1007"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1017"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="1031"/>
            <end position="1041"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP80487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1095" mass="122231" checksum="D8A384BA604A7639" modified="2011-01-11" version="1">MASAMAMASSSILEVPLNNNTESLELDLAEFCGGEGSVEEIVGIFLQEKLPAKFWTRAAMEYWKRDLKQDAISLANQGLQALRDQDRPSECLPLLCQLATYAINLSRGLPTLKLETPEYDTIPPAQPTADETLEKAAHYINEASRIDSRNPLVLDARATLYLAQKNYDQALTACEEILKERPTHLPALLGKARVLFHRKFFRPALKLYQQVLKLAPHVLPDPRIGIGSCFWFLGDQKLARQAWERSLVVNPGKASQGARLLLGLSHFQASKDIHLPDEAQFAAYQKAISDVQAVFKMDRNNAAAAAVLASHLLTANNWTTATKMAERAVQYANTASTAVEARLVLARAFHAQGKLNEAMREYRAAAPPERSVLTSILGVAQILVARNEFGIAINMFENVVRRQPRCIEALANLAALRTHLAFTSSSSTEASSEKTKAKELHEQITRLFATRVKNHSMKPDDEGMMPPRIREVASDPDLYIEIARLSSDTDINRSLKAYRQSLTVREDLGKPIPAMLLNNIGVLEWKNGHLTEAQERIESALAATASAVVGDETEREINERTAVCMLFNLGVICEQAKDKAKAKDIYERILLRHPEYVDAKARLALMYLSEKNYDKTNALLKEALTSQTGNGELRALYTYFLIESNQIKQARDFTVATLKDHDKSDVYALCASGALLYTQARENKAIGPEASLDRATLQLDPQCAFAAQGLAIALAEHVISPSNVNAPSSNGAFAPDASNVRAKNLRDSITILTKVREAHNDGSVYVNLGHCHYLRDEFDRAIENYFTASKRFYDDKNVLVLLYLARAWYQKASKDRSFAALRSALTFVQTAKEHHPKDGAIAFNLALVQQKGLELLVDLPPSKRTLAEIKTAIADAQLAQEAFGELASKPAGTVPFDIDIAHQRKRYGESLLRRTSELSESQEAYESSEIAKIERARQERENERQRLAEAERLRLEEIAAKNEELTRKRQEMQESVATWYIKQANSDNESEDGTKKKRKGGGGKRKNNKETGITIVPDDEVDEGSEQEEKKRKRKRTKKQSNQKEDQTNNRSNRAISTGEDENYDSDDAKPVNRSSKKKSGKIYKSAEFVEDDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>E3K814</accession>
    <name>E3K814_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_06371</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80415.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324834.2">
        <property type="nucleotide sequence ID" value="XM_003324786.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K814"/>
    <dbReference type="STRING" id="418459.E3K814"/>
    <dbReference type="GeneID" id="10539424"/>
    <dbReference type="KEGG" id="pgr:PGTG_06371"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06371"/>
    <dbReference type="HOGENOM" id="CLU_310821_0_0_1"/>
    <dbReference type="InParanoid" id="E3K814"/>
    <dbReference type="OrthoDB" id="3163292at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051089">
        <property type="entry name" value="prtT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31845:SF34">
        <property type="entry name" value="TRANSCRIPTIONAL ACTIVATOR OF PROTEASES PRTT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00172">
        <property type="entry name" value="Zn_clus"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="140"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="180"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="253"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="326"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="791"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="944"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="205"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="329"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="347"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="803"/>
            <end position="813"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="815"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="860"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="945"/>
            <end position="961"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="974"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="993"/>
            <end position="1005"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1016"/>
            <end position="1026"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1060"/>
            <end position="1092"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1100"/>
            <end position="1112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP80415.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1120" mass="119833" checksum="C25285A4604E0364" modified="2012-04-18" version="2">MLEGSFRPSSSATGLHPQYPIPKSGYAEQPSPAANMRKDHPGPSFTPVHDRPLSSHSPDYESEDHQQSGSPPGHAPHFFKSHPSMASHPQSNQSSVHQAASELPQSSSIPKLDGRPPQEVAPKNSNIGPVRAKKKPAGSACNSCHRLKTRCSGGHPCDRCAAHKVACQFDRDLIYRSPLASSSQQASARNAHAMSKGETAPAGKPRSRSGPKAGRKFPPASNPGAVSQSAPTAPMLPMALPQVTQLSAPSLPTLSAPIAHQPGSFSAAPPKQGNKSTPISTPQPPEGYANYEPNLSHPNGLHVRLAALENSVSGLLQVLRAHQSTNPSFLPPAAEPRPPLSPPTPPTLVVSGSSSTFGPEKDQPNDPNRLGAPCATSGLGDPLSEGILPAMQLQYLYDVFVEFCLPSLPILHAVQIASIRARSSFLFSVMVAIGARFCSSQSVAHIPPKNCLMSNSTGARVDLSQVTIDEATYARLVTLAHQHLSNTLLKATHSLEDVRAILFLACWALVSNEDPNGAPNRWVLIGHAGRIGRSIGLDRCTSELRGSSDIWNNPELHETRRVQLDAIKTDHALQAIETILSSNLRLEPSRDYFPPLHVATEREPSFTIVKLSNGSPSQYDVVSAAQIESLAELAEIWAAANSFLSVADDRSRNLTSEQLIELGEHHNKVMDAWAKKWTWHGSSWAPLLGANLRTIRLLSESLRITLNLAFTNVLYAIAAVSRLPPKFHETLAMAIERVNGSSTAVIQAHRDSVREGFSLAFAPDLVTDGLVSAAITVLQLASPIGLPAMIGKPSGKEIKKRTLNPSGSSSNLENLAREERLPTPQSGEDRADVDMNEANASSSGGPQPHILMRPSHESRASSPSSTPRRLSKSSSPRMAFFSADGIAGGNQMLGKKCFMEPPVAAHYIRMATDVLQSSDRSQTRLGTIMASKIMKLATQAGLMGSPSSSNLVGPTSNTSHSPGGGMVSHDERPSISSTSSLSSTRHATISSAEHPDVNPHPKSKQDGVIGLDSRATDVSNPESSLRQAEDDDEISGENIHTASQQRAVLIAQPGPQDPDFHDDENEDEPTEGNEMADDNCDGDGEADEEDQITGDSYSAIHHEQQPSEKDGQDMMEEDQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K876</accession>
    <name>E3K876_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_06650</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80694.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325113.2">
        <property type="nucleotide sequence ID" value="XM_003325065.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K876"/>
    <dbReference type="GeneID" id="10539712"/>
    <dbReference type="KEGG" id="pgr:PGTG_06650"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06650"/>
    <dbReference type="HOGENOM" id="CLU_1687509_0_0_1"/>
    <dbReference type="InParanoid" id="E3K876"/>
    <dbReference type="OrthoDB" id="10287585at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171806" evidence="2">
        <location>
            <begin position="23"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP80694.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="156" mass="16976" checksum="D8815FD015BF550D" modified="2012-04-18" version="2">MKNINVSTVALVLGALISSTVATSRTITREEWQSRPIFDYDAPLEENWVEPIRFEGPSITRKSFRAGDGVVMLELNSDSDQKRVSIASTGKRGAYYIAQDVDTGNWLGEVPILVGEVHLLDGVEKIYLFLKKKAGPAPPAAGSEELSGKSLPQLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3K8A5</accession>
    <name>E3K8A5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06679</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80723.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325142.2">
        <property type="nucleotide sequence ID" value="XM_003325094.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K8A5"/>
    <dbReference type="STRING" id="418459.E3K8A5"/>
    <dbReference type="GeneID" id="10539742"/>
    <dbReference type="KEGG" id="pgr:PGTG_06679"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06679"/>
    <dbReference type="HOGENOM" id="CLU_095781_0_0_1"/>
    <dbReference type="InParanoid" id="E3K8A5"/>
    <dbReference type="OrthoDB" id="10604540at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP80723.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="254" mass="24940" checksum="62B6DE8704F4D583" modified="2012-04-18" version="2">MQSHDANLRSKGRNNGPPHWPGSLINSHSNSYTSPLYPCNLHFVVFLNSVNMRSFAVLAALVICLSQVTVDVTASTSKPTKTDYFGGRPARRSSLPSTLLTEVLCAAGPTPPPGAGAGGPTPPPPPPAPEAEAPAPSPNGTAVSPTSHPAPAGPPGPVPGDMSSGQCMCPPPPSCAAGGAGTPPAPSGGAADPSTQPAPADNSTAPEPSAPQPDAPTNGTDVPTGDSAGAAAVASPEAMTFTGIVAMSLAAIAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3K912</accession>
    <name>E3K912_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06629</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325092.1">
        <property type="nucleotide sequence ID" value="XM_003325044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K912"/>
    <dbReference type="GeneID" id="10539690"/>
    <dbReference type="KEGG" id="pgr:PGTG_06629"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06629"/>
    <dbReference type="HOGENOM" id="CLU_1960654_0_0_1"/>
    <dbReference type="InParanoid" id="E3K912"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP80673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="128" mass="13484" checksum="F1E60EB30FE20194" modified="2011-01-11" version="1">MEIYSLLALAAGLQTRSDVVVGDVLSKCCFPTLDGCWCAAGCTHRGIGGGHGGHHETHQEGEGEPRAEDRHGGGGMHKFSQLTVYRRNEEIMAEESTQSKPPKISSSGGLVPRPAPGLAPCSQVGFVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>E3K945</accession>
    <name>E3K945_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13 14">Diphosphomevalonate decarboxylase</fullName>
            <ecNumber evidence="3 13 14">4.1.1.33</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PGTG_06637</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>(R)-5-diphosphomevalonate + ATP = isopentenyl diphosphate + ADP + phosphate + CO2</text>
            <dbReference type="Rhea" id="RHEA:23732"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57557"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.1.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 14">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13 14">Belongs to the diphosphomevalonate decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.33" evidence="3 13 14"/>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325100.1">
        <property type="nucleotide sequence ID" value="XM_003325052.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K945"/>
    <dbReference type="FunCoup" id="E3K945">
        <property type="interactions" value="337"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K945"/>
    <dbReference type="GeneID" id="10539698"/>
    <dbReference type="KEGG" id="pgr:PGTG_06637"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06637"/>
    <dbReference type="eggNOG" id="KOG2833">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040369_4_2_1"/>
    <dbReference type="InParanoid" id="E3K945"/>
    <dbReference type="OMA" id="LTLHAMM"/>
    <dbReference type="OrthoDB" id="10253702at2759"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00100"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004163">
        <property type="term" value="F:diphosphomevalonate decarboxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000018">
        <property type="entry name" value="Diphosphomevalonate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.890:FF:000005">
        <property type="entry name" value="Diphosphomevalonate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005935">
        <property type="entry name" value="Mev_decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029765">
        <property type="entry name" value="Mev_diP_decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053859">
        <property type="entry name" value="MVD-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041431">
        <property type="entry name" value="Mvd1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01240">
        <property type="entry name" value="mevDPdecarb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10977">
        <property type="entry name" value="DIPHOSPHOMEVALONATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10977:SF3">
        <property type="entry name" value="DIPHOSPHOMEVALONATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18376">
        <property type="entry name" value="MDD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22700">
        <property type="entry name" value="MVD-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015950">
        <property type="entry name" value="Mev_P_decrbx"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9 13 14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12 13 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 14" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="11 14" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="8 14" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="10 14" id="KW-1207">Sterol metabolism</keyword>
    <feature type="domain" description="Diphosphomevalonate decarboxylase-like N-terminal" evidence="16">
        <location>
            <begin position="9"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Mvd1 C-terminal" evidence="15">
        <location>
            <begin position="206"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR015950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU363086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF18376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF22700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EFP80681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="427" mass="46894" checksum="F62A030A0B7FFC6A" modified="2011-01-11" version="1">MVKEITCSAPVNIAVIKYWGKRDKKLILPTNSSLSVTLDQHDLRSTTTARLLEPSHKNGQVGEEEEDQLWLNGARQPIEKDSRLSNCLKELRKLKAHFELQQPKTEASLPESRRALLIASENNFPTAAGLASSASGFAALVYTISKLYELPIEMTELSKIARQGSGSACRSIFGGFVSWEMGAASDGSDSMAVSVAERSDWPDLEALICVVSDRKKGTSSTSGMDGSVQTSELLQHRIEKVVPERMKRMKSAIKQKDFDSFAALTMADSNQFHAVCLDTQPPIFYLNDVSRSIIAVIEELNRASKAEGDGCLAAYTFDAGPNAVIYAPKRNMRKLLNLILHYFPLPDSDPFTDPKAYFDLSKETPGQLALPIHFNHNISPVWKQGSISRLIHTQVGDGPQVLNNQLGQGLLTVDGLVKSLKNPTNGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3K9A9</accession>
    <name>E3K9A9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome P450-dit2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_07113</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP80861.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325280.2">
        <property type="nucleotide sequence ID" value="XM_003325232.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K9A9"/>
    <dbReference type="STRING" id="418459.E3K9A9"/>
    <dbReference type="GeneID" id="10539885"/>
    <dbReference type="KEGG" id="pgr:PGTG_07113"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07113"/>
    <dbReference type="eggNOG" id="KOG0157">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_27_2_1"/>
    <dbReference type="InParanoid" id="E3K9A9"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24296">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="386"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP80861.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="463" mass="52731" checksum="0343E3754B6E58CF" modified="2012-04-18" version="2">MPGLRLVDISKPEWLEYIQKTNFHNYVKGSRFHSVMTDVFGNGIFVTDGQLWKNSRHILAPLFTVKSFKACISPSLRVNLDTLIEGLELASESRPTVDLCDVLFKFTLNFIVYTTLGKNMGQLDQLHLPPIRPLSPSPDSTFVDAFDFAQNQLDFRFSIITLWKVFERVNIRLGNKIRSACKTLHRYASSLIDERLASFDQDAQRYHETPTDLLGFSLKIRKEMGGELSREELKDAFLNLIIAGRDSTGEALTWAFYHLLMNEDLVAKIREEASTITGKDRAGQVTYENNKEFKWAHAVVLETLRLHPSIPKNIRFALKADKIPGGPVIEAGDAVRWSDWAIARDPEVWGEDCLEFRPARWIDENGNIKQFSQFKFHAFNGGPRVCLGMNFAIFQCVCLIVEVFHNFELEFAPGWLQQVPKSPIVGDSSTSSNLYRTPHYKPSLTLPMSQPMMVTIKSYQGDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3K9H8</accession>
    <name>E3K9H8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNL-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_07338</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP81086.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325505.2">
        <property type="nucleotide sequence ID" value="XM_003325457.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K9H8"/>
    <dbReference type="STRING" id="418459.E3K9H8"/>
    <dbReference type="GeneID" id="10536408"/>
    <dbReference type="KEGG" id="pgr:PGTG_07338"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07338"/>
    <dbReference type="HOGENOM" id="CLU_1230427_0_0_1"/>
    <dbReference type="InParanoid" id="E3K9H8"/>
    <dbReference type="OrthoDB" id="512667at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050821">
        <property type="term" value="P:protein stabilization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024158">
        <property type="entry name" value="Mt_import_TIM15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007853">
        <property type="entry name" value="Znf_DNL-typ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20922">
        <property type="entry name" value="DNL-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20922:SF13">
        <property type="entry name" value="DNL-TYPE ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05180">
        <property type="entry name" value="zf-DNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51501">
        <property type="entry name" value="ZF_DNL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNL-type" evidence="6">
        <location>
            <begin position="106"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="195"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="31"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="64"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="97"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP81086.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="225" mass="25156" checksum="B0C8064CBB1BD1F8" modified="2012-04-18" version="2">MLSSTRNLTRLATRQLRNLSQKPAPIHSRKISQLTQQPSPVSEPTTYHTRRYNSTTSLSNPTLPEKEQQQEGETHDDQQSSTPGPSRMRIQFKCIAPSSSSSTSSLDPPPSDQQEATTTCDHLNTHEFSSQAFHHGIVLVQCPSCLNRHLIADHLQWFTSNRTSDDPNFKDDHRTIIDLMRAKGEAVKRGRVITSFQSNPTSQSPSSPSSQSDSPNVGEVLEFYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>E3KA93</accession>
    <name>E3KA93_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_07005</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81384.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325803.2">
        <property type="nucleotide sequence ID" value="XM_003325755.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KA93"/>
    <dbReference type="STRING" id="418459.E3KA93"/>
    <dbReference type="GeneID" id="10537038"/>
    <dbReference type="KEGG" id="pgr:PGTG_07005"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07005"/>
    <dbReference type="eggNOG" id="KOG4412">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000134_18_2_1"/>
    <dbReference type="InParanoid" id="E3KA93"/>
    <dbReference type="OrthoDB" id="539213at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000520">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050745">
        <property type="entry name" value="Multifunctional_regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24189:SF50">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24189">
        <property type="entry name" value="MYOTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13637">
        <property type="entry name" value="Ank_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01415">
        <property type="entry name" value="ANKYRIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="70"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="103"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="181"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP81384.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="245" mass="26215" checksum="93849A9266A615DA" modified="2012-04-18" version="2">MPIDIHRSAYEGQAQLVSAALAEDPNRINALDSDGRTPLHNSSSSGSLSVVRVLLEQQEPKCDIEIPDAMGWTALIIAASAGITEVVSELIHAGANVNAVNQKGQTALHYAASKGRLEIGRLLVQYGADINAKDRANQLPLHRAASSGATPFVKLLLELPNADEEKGTERPKAKMNVVDNAGHTPLHLAFESGHAETACVLIEAGANRERLDPDGKRPDQLVEVLGDVASKRILQYVEARCGKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAW4</accession>
    <name>E3KAW4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_06975</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81354.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325773.2">
        <property type="nucleotide sequence ID" value="XM_003325725.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KAW4"/>
    <dbReference type="STRING" id="418459.E3KAW4"/>
    <dbReference type="GeneID" id="10536926"/>
    <dbReference type="KEGG" id="pgr:PGTG_06975"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06975"/>
    <dbReference type="eggNOG" id="ENOG502SFDN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_478273_0_0_1"/>
    <dbReference type="InParanoid" id="E3KAW4"/>
    <dbReference type="OrthoDB" id="2496288at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01389">
        <property type="entry name" value="HMG-box_ROX1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009071">
        <property type="entry name" value="HMG_box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051356">
        <property type="entry name" value="SOX/SOX-like_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45789">
        <property type="entry name" value="FI18025P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45789:SF2">
        <property type="entry name" value="FI18025P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00505">
        <property type="entry name" value="HMG_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00398">
        <property type="entry name" value="HMG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50118">
        <property type="entry name" value="HMG_BOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HMG box" evidence="5">
        <location>
            <begin position="342"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="HMG box" evidence="3">
        <location>
            <begin position="342"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="235"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="312"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="421"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="526"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="254"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="332"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="435"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="538"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP81354.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="570" mass="63399" checksum="31EDE61FF68B5206" modified="2012-04-18" version="2">MDFQEESADPFDEILRSIKASSQDSSPEEEAPSHDFHDNVDQTTDEHKASPVEFYPSIADTFPSEVFGEGHTSVFPPDLTSPFQAMSGPTLAMEPPTSHGNAFHFLDFSTANFEISRPESSFESHLKTSLDFQNERAMFPSSSAEILSASPNSSPEAFTAFPFSSHIYGDHLPFLYPEELSFHSSPTQIPSSSPDSSPGAFAAFPMSSHNLYGDHMPFTYPKDFGVPIARSHIRGHSDGMPGGRRGSRHHRDLSGTSSTTSTISGPPYDFCSAIDPSALQPPLIKLQTELGFANSETSQIISPFINQVIEQGPDGTITGRYSRPLTSTGRPSHARKTPPGHVKRPRNAFILFRSYACSSNLIPPAVEKDHRQISRIVSHMWKSLPADERLKWERKAEEEKDLHRKLHPDYRYKPIYRKDNAAKKNQGRLATSSAARKKGVSRKNAKGLDASDCGDFSAESTNFGAFHGKSHADLLRERDEEIRCEAVAKVLMEARLSGITLEDGEMEERVQEEVRLVKLNEPHQFQTQPQSEIGEGDQITSSFDQNSESTFPSFDDYRILFPNPIPENHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBA7</accession>
    <name>E3KBA7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein transport protein SEC31</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Protein transport protein sec31</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGTG_07869</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">COPII-coated vesicle membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the WD repeat SEC31 family.</text>
    </comment>
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        <property type="protein sequence ID" value="EFP81620.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003325991.2"/>
    </dbReference>
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        <property type="interactions" value="448"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KBA7"/>
    <dbReference type="GeneID" id="10533163"/>
    <dbReference type="KEGG" id="pgr:PGTG_07869"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07869"/>
    <dbReference type="eggNOG" id="KOG0307">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003033_2_0_1"/>
    <dbReference type="InParanoid" id="E3KBA7"/>
    <dbReference type="OrthoDB" id="542917at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0000318"/>
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    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090110">
        <property type="term" value="P:COPII-coated vesicle cargo loading"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007029">
        <property type="term" value="P:endoplasmic reticulum organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.940.10">
        <property type="entry name" value="Functional domain of the splicing factor Prp18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040251">
        <property type="entry name" value="SEC31-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13923">
        <property type="entry name" value="SEC31-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13923:SF11">
        <property type="entry name" value="SECRETORY 31, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="10" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="11" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <keyword evidence="7 13" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="140"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="13">
        <location>
            <begin position="313"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="22"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
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            <begin position="392"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
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            <begin position="457"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
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            <begin position="567"/>
            <end position="670"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1027"/>
            <end position="1052"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1121"/>
            <end position="1399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="466"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="573"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="616"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1182"/>
            <end position="1192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1224"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1242"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1293"/>
            <end position="1320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1329"/>
            <end position="1338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1351"/>
            <end position="1374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1383"/>
            <end position="1399"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EFP81620.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1501" mass="160142" checksum="347A9D5729628D70" modified="2012-04-18" version="2">MRYTSLPRTATFAWSPDPFLPASQNPSSSASANTHSEHQAELDQFNSPLLATGTASGVLDSSFSTDSKLEIWSPFTSDSAEAKGTVTLQSRFHRLAWGHAAGLRPRGVIAAGMENAELDIWDPIEILAGQGPEKALVSKMKNHNGPIKGLDFNPVKNNLLASGASKGEVFIWDLNNPVKPFAPPSSRSLDDVSSVNWNHIVQSVLAASSNNGYTVVWDIREANGQKGREVATLSYSGSVNQMPAYGQPQPGMMGGPQYGSQWMGPAGGRPGSVSSVVWHPENPTKLVTASDDDASPIVLVWDLRNARAPERILAGHEKGILSLAWCKQDSDLLVSCGKDCRTICWNPTSGEIVAELPPSSNWSFQSDWCPRNPDLIATASFDGRIAINSLQSTGEESETVEPPSTSGPAVDGSDIFGDQGLLALNSAKAAVSCKQAPKWYKRPSAATFAFGGKVVTIDNPSPPNPTQQAPGDNTSIKTSPVVKIRQVVTEPTLVERALKLENASQARNLQTLCEERANRLPSHASTIELQSWKLLSTLFKSDSSEELVNLLGFSKSEVKSMVERQIESYKPSKVSNSLSRTASEMGPTSTALETSPSDNDSIPREPLVTFAETPREGLSASSEGGGEATVSSADAAPSEASVSAISDGTKLAEAESEITEPSLFGDDNANAAGQTAASVDFYNSMRTNRPAGLPDHVFSRVSAAASSVGATIGSQTSSVASEITRSNTFQIYPSEESKGDRLITRALVVGDFESAVSLCISSERWADALLLGVRGGPELLQKAQKAYFGKQTVNHPYLRLYQSIVLDDLMDVVQNADLSEWQEIFVVLCTFAKKEEFSNLVEQLGQRLEYMSHPSTPTESPGQQPSSVSTAEFRKNAILCYLAAGKLEKVTSIWIEQMEEEEAALLKNVDADDQQSAFSIHADVLQTLIEKVTVFQSAINYVDLDLAQTNESVGATKTDAKEYKLAMLYDCYYEYAELLASQGLSNTALRFVAQTPLGYTPSRTSPSSSARERLSKSVGLTSLVADRQFPAEQRTPTSATAVPPLDKPFAEVPSTQNAYQNSYNPSNNLSAYSNTAYQPSSMGAPGGNIDGNYSNPYGSSYNQMPMGQNNYAPMSTGSFLPPPPAPIGTSPIPSRAPSVSQLPPAARQPPSQVGGWNDAPVVNNTRKNVPPPGGPKAVISSPFPNASQTMSPAQPYPNQFGGYGAPQTGYQQGSPGGPPPPPRGGSRTPGNAANVVPPPPKFGQHPPGGVPQTPQQYGQPGGNVAPQSGHPGPYGPPKAQHPGAGPPAQPRQVNFSTPTQQASYQAQPVMQSPMQQQTPYGSGPPPQMYPQQRGSLSGAQAAGQYGIPPAGQGMPPSGQNMPPGQGPPQSQRPGTAPPVPKPEPPKSKYPPGDRSHIPEASKPIYVSLQRLMNQMKQGLPANQKKLVDDTERRLNVLFDALNCETVPASVIDELTDIVKAIEARNLQLALQLHVSLLTSGIKSDQLTTYMPAIKMLITKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBL7</accession>
    <name>E3KBL7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07948</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81699.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326118.2">
        <property type="nucleotide sequence ID" value="XM_003326070.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KBL7"/>
    <dbReference type="GeneID" id="10533406"/>
    <dbReference type="KEGG" id="pgr:PGTG_07948"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07948"/>
    <dbReference type="HOGENOM" id="CLU_2942853_0_0_1"/>
    <dbReference type="InParanoid" id="E3KBL7"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173726" description="Secreted protein" evidence="1">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81699.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="60" mass="6635" checksum="80514CE1073FE444" modified="2012-04-18" version="2">MKGWAPAVRVLRGPPRWLLPWTWTNHLLLALCRPSCSDTAVRTVVGQIVRPVSHPVGPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBT0</accession>
    <name>E3KBT0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="5">40S ribosomal protein S16</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_07974</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326144.1">
        <property type="nucleotide sequence ID" value="XM_003326096.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KBT0"/>
    <dbReference type="FunCoup" id="E3KBT0">
        <property type="interactions" value="184"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KBT0"/>
    <dbReference type="GeneID" id="10533433"/>
    <dbReference type="KEGG" id="pgr:PGTG_07974"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07974"/>
    <dbReference type="eggNOG" id="KOG1753">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046483_4_0_1"/>
    <dbReference type="InParanoid" id="E3KBT0"/>
    <dbReference type="OMA" id="WPIEMAR"/>
    <dbReference type="OrthoDB" id="426865at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000007">
        <property type="entry name" value="40S ribosomal protein S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_uS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_uS9_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001749">
        <property type="entry name" value="PRK00474.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569:SF16">
        <property type="entry name" value="RIBOSOMAL PROTEIN S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP81725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="143" mass="15773" checksum="98B58682B843D9D8" modified="2011-01-11" version="1">MSTSQSVQTFGKKKTATAVAFCKDGKGLIRINGTPIALVQPEILRYKVYEPILIAGADAFSKLDIRVRVTGGGHTSQVYAIRQALAKAVVAFYAKYYDAASALALRKVLIQYDRTLLVADPRRMEPKKFGGKGARARFQKSYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCC8</accession>
    <name>E3KCC8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08125</name>
        <name evidence="3" type="ORF">PGTG_15906</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EFP81876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="DS178328">
        <property type="protein sequence ID" value="EFP90058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326295.1">
        <property type="nucleotide sequence ID" value="XM_003326247.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334477.1">
        <property type="nucleotide sequence ID" value="XM_003334429.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCC8"/>
    <dbReference type="GeneID" id="10533913"/>
    <dbReference type="GeneID" id="10546036"/>
    <dbReference type="KEGG" id="pgr:PGTG_08125"/>
    <dbReference type="KEGG" id="pgr:PGTG_15906"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08125"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15906"/>
    <dbReference type="HOGENOM" id="CLU_2237892_0_0_1"/>
    <dbReference type="InParanoid" id="E3KCC8"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP90058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="105" mass="11442" checksum="8D58BCBEBB26FE4A" modified="2011-01-11" version="1">MTSIAENHSPSITFAGDSADFPIVIADDPAKSPTQAISPVTAQKSANKTVEKKSPEKGPKASTSGNQEVRRSRRIFRQDEGLGRFIIPPGYLLSNEINIKDHHRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCM2</accession>
    <name>E3KCM2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">tRNA (guanine(26)-N(2))-dimethyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.216</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">tRNA 2,2-dimethylguanosine-26 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">tRNA(guanine-26,N(2)-N(2)) methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">tRNA(m(2,2)G26)dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_07571</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>guanosine(26) in tRNA + 2 S-adenosyl-L-methionine = N(2)-dimethylguanosine(26) in tRNA + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43140"/>
            <dbReference type="Rhea" id="RHEA-COMP:10359"/>
            <dbReference type="Rhea" id="RHEA-COMP:10360"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74513"/>
            <dbReference type="EC" id="2.1.1.216"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.216" evidence="7"/>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82174.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326593.2">
        <property type="nucleotide sequence ID" value="XM_003326545.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCM2"/>
    <dbReference type="FunCoup" id="E3KCM2">
        <property type="interactions" value="911"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KCM2"/>
    <dbReference type="GeneID" id="10529546"/>
    <dbReference type="KEGG" id="pgr:PGTG_07571"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07571"/>
    <dbReference type="eggNOG" id="KOG1253">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010862_4_0_1"/>
    <dbReference type="InParanoid" id="E3KCM2"/>
    <dbReference type="OrthoDB" id="6349953at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160104">
        <property type="term" value="F:tRNA (guanine(26)-N2)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002940">
        <property type="term" value="P:tRNA N2-guanine methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000450">
        <property type="entry name" value="N2,N2-dimethylguanosine tRNA methyltransferase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.56.70:FF:000001">
        <property type="entry name" value="tRNA (guanine(26)-N(2))-dimethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.70">
        <property type="entry name" value="N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002905">
        <property type="entry name" value="Trm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042296">
        <property type="entry name" value="tRNA_met_Trm1_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00308">
        <property type="entry name" value="TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631">
        <property type="entry name" value="N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631:SF3">
        <property type="entry name" value="TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02005">
        <property type="entry name" value="TRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51626">
        <property type="entry name" value="SAM_MT_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="1 12" id="KW-0820">tRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="90"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="557"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="166"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="579"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="627"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00077143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00082896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00083299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EFP82174.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="717" mass="77999" checksum="1F3DE00BDB317077" modified="2012-04-18" version="2">MNHIRRRLLISITLNQHHNHQARRLMSNFPEPPPGYQAHQEASTQILIKLEKQEQQPSEAFINPVQEYNRDLSISAIKAWSSIRNQELLEKKSNNSSKNQRKKNSNNKRAVDPSPENGTTDHHHQSKKLKQSSGSDHQSNPTIQEDPTSTISKESVDQAGQPLEPQVDRNPKKETQPQKFTALEALSATGLRAIRYAKEIPTLKSIIANDLSLSAVNLIKANITHNGLDPKDPSTEQAKVRVNQGDACDLMYSHRAPDRQFDVVDLDPYGTAAPFIDAAVQCVRNGGLLCVTCTDLAVLAGSAYPEKCFSNYGGSSMRAEYSHEYALRQVIHSLASSAARYGKQITPLLSLSIDFYVRIFVRVNHSPAEVKKWITNTALVYVCSGCAAFHFQPLGRMNSKPTKAGNGANVSYQNAHGPPMPGASCEECGGTFHVAGPLWSGPLHDPAFCDRLLEGLEAADDEQQQQPLKTAERIRGMVGLAKTELPEAPFFFTPAKLSGLFKCSSPSLLTVGSAILNAGFSLSRSHCQPGSIKTDAPRAFIYDLMKAWINAQAAPVPKSTKDLGAGDQPAAVDGGNAPRVDESLDGEVKNGDVRNGEVKDGGVQDGEVKSGEVKEGEVKNGQAEDEGGLKAEKVKEGQKSGGAGEPAAEISEACRVHGAKNSSNPRLMSLLSKPMRYQVDLKYNKSVEKLLLTGLKIVRYQANPTPNWGPKARALKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCP9</accession>
    <name>E3KCP9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_07598</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82201.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326620.2">
        <property type="nucleotide sequence ID" value="XM_003326572.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCP9"/>
    <dbReference type="GeneID" id="10529573"/>
    <dbReference type="KEGG" id="pgr:PGTG_07598"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07598"/>
    <dbReference type="HOGENOM" id="CLU_2543687_0_0_1"/>
    <dbReference type="InParanoid" id="E3KCP9"/>
    <dbReference type="OrthoDB" id="10274558at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP82201.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="83" mass="8872" checksum="1695453B4BE909C6" modified="2012-04-18" version="2">MTVRNASSNFKGSMSCNMARSRVGSSKATLLLDVKSGIESCFAGHADNRPGHFKLYDATLALYYVLKRPSIPMPSSGDSKGSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDN1</accession>
    <name>E3KDN1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_08423</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82467.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326886.2">
        <property type="nucleotide sequence ID" value="XM_003326838.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDN1"/>
    <dbReference type="GeneID" id="10529846"/>
    <dbReference type="KEGG" id="pgr:PGTG_08423"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08423"/>
    <dbReference type="HOGENOM" id="CLU_894689_0_0_1"/>
    <dbReference type="InParanoid" id="E3KDN1"/>
    <dbReference type="OrthoDB" id="10274320at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173263" evidence="2">
        <location>
            <begin position="19"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP82467.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="274" mass="30305" checksum="D8656576B28DB010" modified="2012-04-18" version="2">MKLQLAAIISVLLSLAAGGSVARFSSTKEEARRLSRGISDDEHSNIRASLAGSSSQAVGQRPQPMNRNFLLTVPKYARADGVQGYCSLTICRSIAPPAFEEPGERYPHYYYSAQSGWHDAELLFKAIIMNGNKKAVLRLESCSTTRVVTVRLCYPSTNTIYPAIELPPRGSIGVEVPRDAEWPRKNAPVLHLLGVKDASRRSSSHHQLSAASPEYRPIQSYNDRDSQLSRSFQTLQLGPPSAHPPGYKFNNEDEFDQLGFVTRPTFEYISPPSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KE61</accession>
    <name>E3KE61_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08799</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82603.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327022.2">
        <property type="nucleotide sequence ID" value="XM_003326974.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KE61"/>
    <dbReference type="GeneID" id="10529986"/>
    <dbReference type="KEGG" id="pgr:PGTG_08799"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08799"/>
    <dbReference type="HOGENOM" id="CLU_371359_0_0_1"/>
    <dbReference type="InParanoid" id="E3KE61"/>
    <dbReference type="OrthoDB" id="2497888at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82603.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="749" mass="85493" checksum="1AE97E36BDCC3B4C" modified="2012-04-18" version="2">MINALGTAGESMSNAGRAGSTLKSGEELGNTRALAFQKSFENTPMASYQKNPSSAQISGSSRSFGSQPIIKGAKQPELRRKPSWLDDSGIKIDAEYRDPTSKELKEFDKQKLQIIKALDNKEQGFNLGNGKLYNARNLVELLEQKIIPKGQVPGRKMYGNYGSYLQALTIPGEHEAQSAVTTLLNELDRATLAKFQREYELTLLSLASVQNIIPKRISSFFPDLLEKNAIDTDKFAKARAIQLSAKSAEEPSNPIYSTVEEVIHALPADFDLTTELYHYSLMKGFEKILAEEREQIHPLIENTANRQINYVFKNPWSVRLQAESYINKLGEAEFERWSGEHPSTIFQKLTQTPQDPDKILYAVNNRKGSLKTHDYETLREANTGRRAFRKELRIIEQRVSASQDPDKVQYLETLKKLEKEAINLDSINYAYVEELFNKKLIDASTRAKLNPTHGISKQQFMEALGTQEDFKKKLVSQFKNNLVSRIGDPVESSEEMGNVHQKAGDILYKMYFDQLDKKANQFYLTREAFERPYNKDFEKAFKVYDAKVVKKFLQQPDIESITKAYVDAHIPAAIVPDSALERFLNEVHLKSPFPKADGGTISNLLPGLDKRLAEFWHKPLVKDDERLKGTRWETLTEDSREKLNGAAQDIFKHYDVLNKAAEDHDALSKSVRNIENSIYQEIPRINQLFRRISKLAEPPTPKPGPEMSEEEIKQINKKYTDRLSRKKLRDSMGDWVRKIKNFVADKLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KE99</accession>
    <name>E3KE99_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_08828</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327051.1">
        <property type="nucleotide sequence ID" value="XM_003327003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KE99"/>
    <dbReference type="STRING" id="418459.E3KE99"/>
    <dbReference type="GeneID" id="10530015"/>
    <dbReference type="KEGG" id="pgr:PGTG_08828"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08828"/>
    <dbReference type="HOGENOM" id="CLU_004591_6_1_1"/>
    <dbReference type="InParanoid" id="E3KE99"/>
    <dbReference type="OrthoDB" id="2505986at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP82632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="375" mass="43361" checksum="72EA4A052A10E2DE" modified="2011-01-11" version="1">MTHVKETVNLDKLDKKSAEFGVRDQINRKFSKQLFKFDAKKLELLENGEELPTHMDQEIPQELVDMEEKEPKRMFNSFLELKGFDMVEDTPVEILHVFLLGAVKYLFRDFMKGLNELQKDDLLALWNSFNTNSLNIPSVRPKSMVQYASSLIGKDFLIILQAAPFIFFQFMTESKIKIWSSLCHLGSLIFQTHIEDMTEYISELRNAIDIFLRNIIEDSAQWINKAKFHMLLHLPESILRFGPACLFATEKFESYNGILRNASIHSNRQSPGQDIAITFITNIFSQNPLIQQTLGYNQSASLPNKSYPCIKKLSVPAIHQVETPQELKSLYPDFEIKQISELNLNKKQVLKKDYFILVFPEALSSSVSLAKFHHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3KEG5</accession>
    <name>E3KEG5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SHSP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_08894</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327117.1">
        <property type="nucleotide sequence ID" value="XM_003327069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KEG5"/>
    <dbReference type="FunCoup" id="E3KEG5">
        <property type="interactions" value="156"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KEG5"/>
    <dbReference type="GeneID" id="10530081"/>
    <dbReference type="KEGG" id="pgr:PGTG_08894"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08894"/>
    <dbReference type="HOGENOM" id="CLU_046737_12_0_1"/>
    <dbReference type="InParanoid" id="E3KEG5"/>
    <dbReference type="OMA" id="IHGEHRD"/>
    <dbReference type="OrthoDB" id="1431247at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051259">
        <property type="term" value="P:protein complex oligomerization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009408">
        <property type="term" value="P:response to heat"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009651">
        <property type="term" value="P:response to salt stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031107">
        <property type="entry name" value="Small_HSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11527">
        <property type="entry name" value="HEAT-SHOCK PROTEIN 20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="5">
        <location>
            <begin position="47"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="22"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="90"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP82698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="178" mass="19202" checksum="ED8714742E6F943A" modified="2011-01-11" version="1">MSFLIAPSQWDDFEQLFDKLVTDRYGPPADGSKAGSESKAVTSGSHQVSKILRPKMDVVETDSSIVVTTELPGAKKEDISRSISRMAVFLSPDKPKPRANTLKEASDAPSPPPSKSSEDLPLMLGQISERSFGSFSRVIAVPQGLTHDQVKAGFKDGVLEVSIPKTVPNKESHNIPIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3KEI4</accession>
    <name>E3KEI4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PAS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_08913</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327136.1">
        <property type="nucleotide sequence ID" value="XM_003327088.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KEI4"/>
    <dbReference type="STRING" id="418459.E3KEI4"/>
    <dbReference type="GeneID" id="10530100"/>
    <dbReference type="KEGG" id="pgr:PGTG_08913"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08913"/>
    <dbReference type="eggNOG" id="KOG0498">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_371356_0_0_1"/>
    <dbReference type="InParanoid" id="E3KEI4"/>
    <dbReference type="OMA" id="HREPHAN"/>
    <dbReference type="OrthoDB" id="447251at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00130">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.20:FF:000196">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.20">
        <property type="entry name" value="PAS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035965">
        <property type="entry name" value="PAS-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00229">
        <property type="entry name" value="sensory_box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47429">
        <property type="entry name" value="PROTEIN TWIN LOV 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47429:SF2">
        <property type="entry name" value="PROTEIN TWIN LOV 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13426">
        <property type="entry name" value="PAS_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55785">
        <property type="entry name" value="PYP-like sensor domain (PAS domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0157">Chromophore</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PAS" evidence="5">
        <location>
            <begin position="440"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="151"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="358"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="681"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="24"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="42"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="106"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="161"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF13426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP82717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="856" mass="94790" checksum="4343E58499831414" modified="2011-01-11" version="1">MGTPDEDDYQQLIDPTLRDYQHFPTSLSTPPHTPSSKKVPTEYSQQGFTSSSTSLPRDGGLKIHSPNPYYQPGILPPRNLEQNIQTRLHRSTSSASLAPASPPSPSDSNPTNPQTTTLNLSNHLSTRRPRLGPNPSLQDLKSASIKHAHPLPIPTGYLPNSIKSPASYSAGHQTDPTTISHDFQGEKDHTSQTFRSQNQHNNNNNRLEFGEQWMINNENYENNQLSEEDAKPNNKISADLDYSLRRLINLDVFRKLLEDPTSRRAFRQAIILGLSPSSSSAAPPSVSLTKLDLWLDCRGVNQILNLLKIGTHGLIDVYLKDPDSRHALHAILPDSMSIGGGAFNSLLSSINRLKLLANEEQEEEQNDDRIGTDRDGTEEEGVVEGGVASTGGLKAIENRLLISLYQNEFPLFIKKNLVSNVVVKLGKFNLNESDRDGLGDCFCLTNPRLRDHPIVLVSDGFTKVTGYERQAIVGKNCRFLQGPGTSPESVQRIRDGLNSGEGCTELLLNYRKDGTPFYCLLCIIPFRDVTGALIYFIGGQINVTGMLSSQKGLSFLMANNEDAYSHGTNELSRSTGRSAGRSTDDLLDHVGLSQHSFSPTMQHFAMRGHPTNNVSEQTTSSRDLADSTDVRGNPTMGGEYHAAGSITFDDFDPNFGKDNSAFRAKSSSFFRRLAGIHHRDPTNKNLASPYLHSPSTSSKKESQKLLGAETLFHEPKPLSDHIKQFESTYSHLLVFQQDSRRVIFATKEAIALFGLNHSNSKEIYKSKLIQTDFLELVWPVNTTNKESVATLRAHLKKIIKAGLPISFDCQVKSIQRGFFLSITKEEDPSKLVTTRIHLTPLMDKDGKIVAYVCLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KFJ8</accession>
    <name>E3KFJ8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_09151</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EFP83198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327617.1">
        <property type="nucleotide sequence ID" value="XM_003327569.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KFJ8"/>
    <dbReference type="GeneID" id="10544911"/>
    <dbReference type="KEGG" id="pgr:PGTG_09151"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09151"/>
    <dbReference type="HOGENOM" id="CLU_698562_0_0_1"/>
    <dbReference type="InParanoid" id="E3KFJ8"/>
    <dbReference type="OMA" id="ANHEYQQ"/>
    <dbReference type="OrthoDB" id="429145at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172586" evidence="2">
        <location>
            <begin position="20"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP83198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="398" mass="41538" checksum="7D900FAD270BDC4C" modified="2011-01-11" version="1">MVQKFVILLAIGLAFTVSASFEGRSGEATVIQVEEHTGNGPPHHPELPPRSGIITGSSRPTIILDPEPSFVPSQVSQSSVRPSALTQPLAPPQRIIVHQPPAPQGVPIGVRPFNHQQGAGPLAVHPQPPPATQAGVGVPVNYQPAAGLTAGHSQTSSPAPTGARPVNYHQLSAGSSAGQPRLMTPAGVRPINYQQSTVLSVVHSQVPPAVRTWHAPSRQTGPASYQQQGAGHCAVHSQALSGTSVGQASPVPNYPQPVAGRPDVPLQGNPMTSSRVVPPAPTTNEARPVEYIQIDDTPSDPGPPLQASQPAHEPDPSTQPSSSAPPGKEFSLLFGIPYPSLECFVKTHPAPSIPINFVISSGACFKFSPQTSLSAAEIFPPSGSCFPFPSFRKRKKPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3KFK6</accession>
    <name>E3KFK6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase M48 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_09023</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the peptidase M48 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EFP83070.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327489.2">
        <property type="nucleotide sequence ID" value="XM_003327441.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KFK6"/>
    <dbReference type="STRING" id="418459.E3KFK6"/>
    <dbReference type="GeneID" id="10544782"/>
    <dbReference type="KEGG" id="pgr:PGTG_09023"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09023"/>
    <dbReference type="HOGENOM" id="CLU_036521_1_0_1"/>
    <dbReference type="InParanoid" id="E3KFK6"/>
    <dbReference type="OrthoDB" id="7464992at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07331">
        <property type="entry name" value="M48C_Oma1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051156">
        <property type="entry name" value="Mito/Outer_Membr_Metalloprot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22726">
        <property type="entry name" value="METALLOENDOPEPTIDASE OMA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22726:SF18">
        <property type="entry name" value="PEPTIDASE M48 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="1 6" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M48" evidence="8">
        <location>
            <begin position="126"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="82"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="323"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP83070.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="340" mass="38040" checksum="80220BE5965993B5" modified="2012-04-18" version="2">MCLVIAIGIDQSPRTHRWRLLLMSEAEEMEWSRRRFEDLVSTDGDLIVGPQDPRTQMVKEICDRLMTALDLDSPVSAAAWPRNPEIDEQHHFGRRVEPSRKEIKSSATASSDLLPWKPESSNPEKKLESNDWELFIIDSPRINAFVLPTKKIFVYTGLLELIQNSEEMAAAVIAHEVSHVVERHAVENLGFSALSAVVFDAMRGVSYALTISFPLLSDGLAFCINYLNDVVVQKAYSRKLETEADELGLMIMARAGYNPGAAVKLWNLLNRLEEDQETSHGSSGIGWPETIPWTRTHPTCKDRGLTIQKALPKALKYFHQPQPSSFPSNSTTAPSSPSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KH81</accession>
    <name>E3KH81_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09369</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83656.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328075.2">
        <property type="nucleotide sequence ID" value="XM_003328027.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KH81"/>
    <dbReference type="GeneID" id="10532342"/>
    <dbReference type="KEGG" id="pgr:PGTG_09369"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09369"/>
    <dbReference type="HOGENOM" id="CLU_888864_0_0_1"/>
    <dbReference type="InParanoid" id="E3KH81"/>
    <dbReference type="OrthoDB" id="10425699at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83656.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="283" mass="30557" checksum="D2A2D56B686FB24C" modified="2012-04-18" version="2">MTPKPRESDFPLCQDYLDALRLWAGRHNVNRPFYDERLRHPTPVPQQPTPERARSPVPVRSPVSANPTIDLEPIVISSGPPTPVAKSPVARSPSPSPMHVNPAPEVLRPTPPPSVGAAPEHTTPDSSQPARLNFLRTPRPAERGPSEVPDSVCSRAVTPRPVVNSSPVAPTRANPFAASRHFRHTLPARTTLPSIPDEAGDVFDAIPADAVEAFVLGIQGRFETILSASVLPPSIRRSIYSAFLAELRDLRRHRLPVMAVFDARLADYPDGSGNDPAPGSGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KI54</accession>
    <name>E3KI54_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09692</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EFP83979.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328398.2">
        <property type="nucleotide sequence ID" value="XM_003328350.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KI54"/>
    <dbReference type="STRING" id="418459.E3KI54"/>
    <dbReference type="GeneID" id="10532671"/>
    <dbReference type="KEGG" id="pgr:PGTG_09692"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09692"/>
    <dbReference type="HOGENOM" id="CLU_696634_0_0_1"/>
    <dbReference type="InParanoid" id="E3KI54"/>
    <dbReference type="OrthoDB" id="2591449at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="101"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="110"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="347"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83979.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="395" mass="43958" checksum="5F1FE7EC1FDAC463" modified="2012-04-18" version="2">MSTETPAISTPPSSHQPTDLPSPKLEMDDQGPSPMTMLTFFKGPSRNKLAENPVRKSICFGLVSHHPSQQPQPNRHADRPRVVTNWKSESETQREILNSLEQPVFSPTMSHNRSSSSHSHPTDQRIEPLNLKSIPAEVLLPFNERENEIVELLAEPQNLGLYKRLKDRLLQAESWPKFEKGLLRASREEIGDREWIVAIRALIEPLDDHLWVSFRGLIGADGLDDQESKPAKEEDWHVQPFKVRRSSIRSTDGRRRMDSHHSRSISSDSSLSGVGTLESDMEDSFSMVSQSPRARMDSIKEHDFLESELDDPEAPGCDSPGMIPQTGSDSGDDQADDSHVLALKILDGPSDPIPSSSDPKGDVKHAYCLLPHGLKYSFGQVVDHGLGLQSLNVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIS2</accession>
    <name>E3KIS2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_10575</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84197.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328616.2">
        <property type="nucleotide sequence ID" value="XM_003328568.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIS2"/>
    <dbReference type="GeneID" id="10545377"/>
    <dbReference type="KEGG" id="pgr:PGTG_10575"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10575"/>
    <dbReference type="HOGENOM" id="CLU_1949876_0_0_1"/>
    <dbReference type="InParanoid" id="E3KIS2"/>
    <dbReference type="Proteomes" id="UP000008783">
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
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</entry>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178290">
        <property type="protein sequence ID" value="EFP84341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003328712.2"/>
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    <dbReference type="AlphaFoldDB" id="E3KJ66"/>
    <dbReference type="GeneID" id="10545687"/>
    <dbReference type="KEGG" id="pgr:PGTG_10061"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10061"/>
    <dbReference type="HOGENOM" id="CLU_769731_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJ66"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP84341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="406" mass="42590" checksum="9CA8156C65A334AD" modified="2011-01-11" version="1">MSLCYSSSFEELSILINCVATISAMLDWTLDPNPSSFLSPPGAADQPNSSAHTPATTTNANTNTTHDSSNVPIILRYALQSLATLKPPNIDDLIHEAAVEFHLDPRSIYLAISINQCPVKISLSLYRRIAPLSTIYVQHHTPAPIFNTLQPGGPSEPLSGPDTSTSTSSTNTTSAPSTTAATSNNGQTSNSRPPAEQAIHDFLNSFATASTVQNPPQPAPINDPSSAVQNPLNGSSPPTIASLLSSSIPPNGLDSSQPSSSTAPGGPNAITAGAFTNQTNQQNMPTTTGNNRTSKKRFLINSLASANTLNQHPSQHQPQQRGVSQGHDQESSQSRRSKFPFLANRRPSAGSPSSSSTLNPSSSAISIADSPSDHSPRFSANGPPSSHDPSNDIFSMDLFNSLSSSS</sequence>
</entry>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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        <lineage>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178290">
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10162"/>
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    <dbReference type="SUPFAM" id="SSF48371">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="670" mass="74525" checksum="92DD050E0250CCC7" modified="2011-01-11" version="1">MKKQLSRLIKNQLKLNQTLKTKNNNNLLLLKPQTSKNTISFIGSKLANTINSITNNNWTNLDIKHTHTNWKTNPLRTTTTTEHPLNAQLIIIVFDPITEYHLLKQQLTLIKNIHQPLLILLDSPIPANLLKPILFQNEPFLALNHNNTIFFIDSQAALDAIHHFDHDTQAAIDRIRNSGLLELRQAIHTALQPPPSSSAQQKDPPNRLRPALSLAELALDLAEDKVLSIVEETQTASARLAQYHRRLQRLQNLARSVQGPGSSEPSSLKKLLISRLGFFRSLFSSDLVGTRASIILDQLYLDQIEKNLIFQAGQIQIIKQRADEVIGELLRDPILARYRTETMVNDVLRSRATDHDLFLLDQQHDVSSEREKGKEKIQIGSVRIFEQHKTRLLGPGGPVQALQTKATRMSLNTVFLGLGIAALSLASHPPLLLLLPTLPSPLPSLPSPALPAFLALAALSFAQKSWSKSVRSFVQDLDRWNHNLHHHLLEYQQRVGERIFLLSTSSSSSSSSSSSSTTTTTTTASEPTGTTTASEIGNHPSSNQEDQVQQQDVDLIQAIRDNIHARLNVPVAETDQEDEDDKDDDDDDDDEQDDEQQQDDDDDDGTLESTPSGTSSTVNLVDWVRQLRRRLESLKPDLSPPPPSSSSSSSSSSSSSSCYDQPKKSSQQTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJX7</accession>
    <name>E3KJX7_PUCGT</name>
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        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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        <lineage>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84602.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329021.2">
        <property type="nucleotide sequence ID" value="XM_003328973.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJX7"/>
    <dbReference type="FunCoup" id="E3KJX7">
        <property type="interactions" value="142"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KJX7"/>
    <dbReference type="GeneID" id="10546271"/>
    <dbReference type="KEGG" id="pgr:PGTG_10761"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10761"/>
    <dbReference type="eggNOG" id="ENOG502SBA1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057712_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJX7"/>
    <dbReference type="OrthoDB" id="340259at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031080">
        <property type="term" value="C:nuclear pore outer ring"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002306">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037626">
        <property type="entry name" value="NUP37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22806:SF0">
        <property type="entry name" value="NUCLEOPORIN NUP37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22806">
        <property type="entry name" value="NUCLEOPORIN NUP37 P37 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="117"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP84602.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="378" mass="41231" checksum="2DF43241DDEB3F81" modified="2012-04-18" version="2">MGDTLLSSYSFGVEEVYRVKWSSSSPDLLAAGTSNSLQIIRLDQDYAPSLVTSFRLGTRVTHISWGPTVMSEDGKNVTIRIELLIACADQNMRLLTYQAATDSQSQRTSVQCFGQSHSGHSGRITSVAWCPVPGYANVIGSAATDKCVLIWNLESSAEGGNPDLPSSPPAPTLIGPFKSTPVTISFHPTSSSRLMVYDANGAIKLIDWTKPDRPIIICLIEPRTLVNKLYSLGHASDRLGMADWKVDEADVFGALNGNRWAVWDMRSTRAGTPLAAGEVWGPGVVADVFRWCPTNPRMFAISTSSPNASNSGSGAIQVYYLSFLQSPRTVQLPGHPTQTRTRVHDIEWQPMSPEADVLCIATGRQLVWMKLGTRESQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>E3KKD1</accession>
    <name>E3KKD1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Aquaporin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_10915</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>H2O(in) = H2O(out)</text>
            <dbReference type="Rhea" id="RHEA:29667"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84756.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329175.2">
        <property type="nucleotide sequence ID" value="XM_003329127.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KKD1"/>
    <dbReference type="STRING" id="418459.E3KKD1"/>
    <dbReference type="GeneID" id="10526724"/>
    <dbReference type="KEGG" id="pgr:PGTG_10915"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10915"/>
    <dbReference type="eggNOG" id="KOG0224">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020019_9_0_1"/>
    <dbReference type="InParanoid" id="E3KKD1"/>
    <dbReference type="OrthoDB" id="3222at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015254">
        <property type="term" value="F:glycerol channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015793">
        <property type="term" value="P:glycerol transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006833">
        <property type="term" value="P:water transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000027">
        <property type="entry name" value="MIP aquaporin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050363">
        <property type="entry name" value="MIP/Aquaporin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00861">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43829">
        <property type="entry name" value="AQUAPORIN OR AQUAGLYCEROPORIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43829:SF9">
        <property type="entry name" value="AQUAPORIN-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="63"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="101"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="231"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="24"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP84756.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="377" mass="40882" checksum="55FBAED89966F2C9" modified="2012-04-18" version="2">MPSNATEPHNSTKLPIPDLPEVGHTVDYEDTPRSEGSRRHYAGNHHPAEGDGLVLRFKSLTRVFWAEFFGTAILALFGTAANNQVALSNSSAVSNAPAGTYISVAFGWALAVMLGVYVSGGVSGGHINPAITLAMAIFRGFPWKKVPIYWAAQLTGSVTGAALTYWNYRHAIDLFEGGGGARTIEKTGGLFFTNPLSSDNQNNQVLLSKLNCPLTNSCLNRLPYVSNFNCFYNEFLMTALLMIFVVAVGDEGNTAPPKNMGPIIIFWVVFGLASTLGMQTSFSLNPARDLGPRLVTWFAGYGSHVWSVRSHYWFVVAILGPVCGAIVGAFIYDFFIATNQVTCTLHGNNSFPPFLAKFLPAKRNRQEQLNGLESGQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KKE5</accession>
    <name>E3KKE5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10929</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84770.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329189.2">
        <property type="nucleotide sequence ID" value="XM_003329141.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KKE5"/>
    <dbReference type="STRING" id="418459.E3KKE5"/>
    <dbReference type="GeneID" id="10526739"/>
    <dbReference type="KEGG" id="pgr:PGTG_10929"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10929"/>
    <dbReference type="HOGENOM" id="CLU_089400_0_0_1"/>
    <dbReference type="InParanoid" id="E3KKE5"/>
    <dbReference type="OrthoDB" id="28755at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37490">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37490:SF1">
        <property type="entry name" value="GLYCOSYLTRANSFERASE 2-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84770.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="272" mass="30718" checksum="0041CEFB6C4409E6" modified="2012-04-18" version="2">MSSAWCIRHDACTMCGPVRYRSERKLKTHWQALFRSIAFGPLLVLLLIKLIWQPPIGVLMTKMLLATARPAGSVDLVFSYYNEPLMIVYVKHPEVSLGNIANVINADEVIRLDNVGREGGTYLTHILKHYNLAILTDQQGRVIQSGKSNPNILEQAEFGVERVLSLPAIHGLADHTVFMQPKISWHWIAKPQMDLFDPKFNCFLDLASSILRVISFWLVGQDVQSRDQEDSLLASEVVQPRRPGFPPGRRGCTTLPARRIPSWPARWALSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>E3KKM6</accession>
    <name>E3KKM6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_10322</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178292">
        <property type="protein sequence ID" value="EFP84851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329270.1">
        <property type="nucleotide sequence ID" value="XM_003329222.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KKM6"/>
    <dbReference type="GeneID" id="10526825"/>
    <dbReference type="KEGG" id="pgr:PGTG_10322"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10322"/>
    <dbReference type="HOGENOM" id="CLU_1489696_0_0_1"/>
    <dbReference type="InParanoid" id="E3KKM6"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP84851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="181" mass="20625" checksum="2C327DCCE3AF17C7" modified="2011-01-11" version="1">MSTRAPQTKGNFMVIRHQCPGSRQAEMHGAQSLRPGPSILERAATSRTPSRRALYRRSLYLTWGKPIRVDRRRLSALPPPTRAAKRFLESGLEYLYRLNNDEAGVTATGKLIGTQKYLYQWIFLVQFCRPQSHVPPPIWSGLARCIKPSAVIRRWWPCSNVGEHRFGSTSSLSFYIAKVLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3KL73</accession>
    <name>E3KL73_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Large subunit ribosomal protein L32e</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_11217</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL32 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP85048.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329467.2">
        <property type="nucleotide sequence ID" value="XM_003329419.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KL73"/>
    <dbReference type="SMR" id="E3KL73"/>
    <dbReference type="FunCoup" id="E3KL73">
        <property type="interactions" value="479"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KL73"/>
    <dbReference type="GeneID" id="10527025"/>
    <dbReference type="KEGG" id="pgr:PGTG_11217"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11217"/>
    <dbReference type="eggNOG" id="KOG0878">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071479_2_0_1"/>
    <dbReference type="InParanoid" id="E3KL73"/>
    <dbReference type="OrthoDB" id="268693at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00513">
        <property type="entry name" value="Ribosomal_L32_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001515">
        <property type="entry name" value="Ribosomal_eL32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036351">
        <property type="entry name" value="Ribosomal_eL32_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L32 AND DNA-DIRECTED RNA POLYMERASE II, SUBUNIT N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01655">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01393">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52042">
        <property type="entry name" value="Ribosomal protein L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="52"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP85048.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="212" mass="23666" checksum="466B0DC2F51D740C" modified="2012-04-18" version="2">MLDRCTVTKRRTGWMAQGEEPSVLNLLESDSGFGVYKCTASPVADTRSIRVERPGTAGRSGPRPCDEKSGPAVGQVHPDSRPPTMPSKIPIVKKRTKPFIRHQSDRYASVKQAWRKPKGIDNRVRRRFKGQLPMPSIGYGSNKKTRHLMPNGYKKFVVNNPGEVDLLLMHNTTFAAEIAHNVSARKRIEIIEKARILGVKVTNAAAKLRSEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLC5</accession>
    <name>E3KLC5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11269</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP85100.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329519.2">
        <property type="nucleotide sequence ID" value="XM_003329471.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLC5"/>
    <dbReference type="GeneID" id="10527081"/>
    <dbReference type="KEGG" id="pgr:PGTG_11269"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11269"/>
    <dbReference type="HOGENOM" id="CLU_357203_0_0_1"/>
    <dbReference type="InParanoid" id="E3KLC5"/>
    <dbReference type="OrthoDB" id="2502606at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85100.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="736" mass="85165" checksum="1E216A1EAFFA93BE" modified="2012-04-18" version="2">MDAEGHIASLTRDRPPESPTGPHVAKLNKQFGARRDRAVEPDNIQSCRLLPEDGDQKNACRRILSSQDPPSLGRTSYLVLNFFGIELAPVLSSFHCNAQARGIDCSYVSELVIAEPDPGGLVRQLNLKWRWPPDACAKGHTEDYFDEPEAPQHLTFDQWPIPPALTDRANCLNRLLAKFCMYDQVCDLAARPVSSLCAITAPRAVLDGSAVGVDDQFNVLRHTSSSYSFKYVQLSSDILRMLLLIKRLTLFFTLVPNRNCTPVGFENFALEETAETLNHAGAMESRKQKLPFNPQMEAQKKDWHSMIQSYDLLLDYKHNIKENEAQTVHFETINNAVQGLTICHNYLRHIFGVPGESLRDALGVHKDPSERLEESDGAYKSSIWNLISVLETPMNMPEPLHHVQIAGNDCILQTIEYIRKYNLAPAGMAEEIQISFKSQRALEWLTKSSIDIFRQGTKFHNDYHCRPFEEVHLKNHFQLTNFNNLYQDLSKSQQDWCLFQYLAARMNPKAQKAIMEDETSPKGFFKPMLELIQFSRKLKKFLLNELESDNNLLDPRIEFLRDSKVDLVKLIEFITEPALERGMSLFDLKIIVGNMFATVDFILSRFLNGYRDENGSGLQMTKAMEFRKKMKLLRLATNIYIWRNMVFDYDHYISKYEDEGHKIPKSVQEATRNFYWRNLNKYVASYKSMRDNQSKQDYDWKQKIEGNIYYKHITDTYDRECEKLEDLHRNFENSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLI9</accession>
    <name>E3KLI9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">lipoyl(octanoyl) transferase</fullName>
            <ecNumber evidence="3">2.3.1.181</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_11333</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the LipB family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.181" evidence="3"/>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP85164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329583.1">
        <property type="nucleotide sequence ID" value="XM_003329535.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLI9"/>
    <dbReference type="FunCoup" id="E3KLI9">
        <property type="interactions" value="544"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KLI9"/>
    <dbReference type="GeneID" id="10527145"/>
    <dbReference type="KEGG" id="pgr:PGTG_11333"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11333"/>
    <dbReference type="HOGENOM" id="CLU_035168_1_0_1"/>
    <dbReference type="InParanoid" id="E3KLI9"/>
    <dbReference type="OMA" id="GEVTYHC"/>
    <dbReference type="OrthoDB" id="19908at2759"/>
    <dbReference type="UniPathway" id="UPA00538">
        <property type="reaction ID" value="UER00592"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033819">
        <property type="term" value="F:lipoyl(octanoyl) transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009249">
        <property type="term" value="P:protein lipoylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000544">
        <property type="entry name" value="Octanoyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020605">
        <property type="entry name" value="Octanoyltransferase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00214">
        <property type="entry name" value="lipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10993:SF7">
        <property type="entry name" value="LIPOYLTRANSFERASE 2, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10993">
        <property type="entry name" value="OCTANOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21948">
        <property type="entry name" value="LplA-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01313">
        <property type="entry name" value="LIPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="6">
        <location>
            <begin position="76"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP85164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="309" mass="35321" checksum="DAD34241FC8DCA74" modified="2011-01-11" version="1">MREALHETIRKGLSGLGRLSRPNHYQLRFTSSSSHHQHEPSTRPRPIIWTYLKDLVRYQDGYEFQNSLVRHKIDHPSAPDWLILLQHHPVYTTGRRQLSDHQLELERSRLSILNPRADFFPTLRGGQITFHGPGQLVAYPILNLGLMNLNTRMYVDFLMNLLRSILVHPTLPRPIISLNPADNPDLPVGIFIGSQWCKIASVGIQVRRRITSHGFALNIEQECERGFKEIVACGLRNTQLTSIQSVLAHPQPRITVNDLVRPTVDLFGELSGIHMKQMDEDCQELDPIGYQLVQKFINSQQQPSQIASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>E3KP82</accession>
    <name>E3KP82_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Large subunit ribosomal protein L18e</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_12063</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="term" value="F:structural constituent of ribosome"/>
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    </dbReference>
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        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="60S ribosomal protein L18"/>
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        <property type="entry name" value="Ribosomal_eL18/eL18-A/B/_CS"/>
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        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
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    </dbReference>
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        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL18"/>
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    </dbReference>
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        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
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    </dbReference>
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        <property type="entry name" value="RIBOSOMAL_L18E"/>
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    </dbReference>
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    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="4">
        <location>
            <begin position="2"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF17135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP86107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="183" mass="20686" checksum="FD10A7D34DF86DF9" modified="2011-01-11" version="1">MGVDIVKHHVRKGHRTAPKSEDPYLLLLVKLYRFLARRTESSFNKVVLRRLYMSKINRPPMSVSRIARTVKDTPGKTVVVVSTVTDDVRLVEVPKLSIAALRFTATARARILAAGGECLTLDQLALRCPTGSNTVLLRGKRNSREAVRHFGHGPHKHKRPYVLSKGRKFEKARGRRKSRGFKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPK5</accession>
    <name>E3KPK5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12196</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178299">
        <property type="protein sequence ID" value="EFP86240.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330659.2">
        <property type="nucleotide sequence ID" value="XM_003330611.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KPK5"/>
    <dbReference type="GeneID" id="10540251"/>
    <dbReference type="KEGG" id="pgr:PGTG_12196"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12196"/>
    <dbReference type="HOGENOM" id="CLU_990899_0_0_1"/>
    <dbReference type="InParanoid" id="E3KPK5"/>
    <dbReference type="OrthoDB" id="2507196at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="149"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="236"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86240.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="277" mass="30632" checksum="0CCD50216FDE808E" modified="2012-04-18" version="2">MPSGSHVKEGSKPLSLTPVPSIIFELFHRRPSARLSFKNTSHLAWALSIGQKACWFRQLHSLTSPELTSFFATLEPWSAQPPLYQPTQLNHACLFLLYIFNQPPSPSSSSSPSSSSPTHSNPLNNLPQPPLVTQHPHHSHYDMAPPTRSISPDSLQSSISSGTCHSFGDPSASSPIHPTLFIRDPRRKNPRNAHQRHQLTESDFGFRSPSSLSTKLRRLLSSPAKAIFNKQVLQHPQSPSSTTSPALPSCLPSSPLSPTPHRKFITDDSFDHQILKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQC8</accession>
    <name>E3KQC8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12459</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178300">
        <property type="protein sequence ID" value="EFP86503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330922.1">
        <property type="nucleotide sequence ID" value="XM_003330874.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQC8"/>
    <dbReference type="GeneID" id="10540518"/>
    <dbReference type="KEGG" id="pgr:PGTG_12459"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12459"/>
    <dbReference type="HOGENOM" id="CLU_003292_5_1_1"/>
    <dbReference type="InParanoid" id="E3KQC8"/>
    <dbReference type="OMA" id="SSNDHEW"/>
    <dbReference type="OrthoDB" id="3254696at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052925">
        <property type="entry name" value="Phage_Integrase-like_Recomb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34605:SF3">
        <property type="entry name" value="P CELL-TYPE AGGLUTINATION PROTEIN MAP4-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34605">
        <property type="entry name" value="PHAGE_INTEGRASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="235" mass="26116" checksum="3B5ABC805CAC423E" modified="2011-01-11" version="1">MLKASSKEDELVGKRAKKLPMMLWHMTHLWKELSDGSDFDKAVLDVAIVAFWGLARLSELSYDSEFGAVSFTSSVLTSDVVFDPENNSFATIVIQNAKTGAPGKPQLITLREQDHVLCPILALKRRLSSSLGTTTTLFGNMEGGVRHHLTRRKTVARVEQVLVDSGYAGLHGHSFRVGGASFRLALGMSTPDLCSLGRWKSDCYKLYVRPYDQDTKKKTLVLLSPLKSNWRKMGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KRD8</accession>
    <name>E3KRD8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_13245</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178302">
        <property type="protein sequence ID" value="EFP86863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331282.1">
        <property type="nucleotide sequence ID" value="XM_003331234.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KRD8"/>
    <dbReference type="GeneID" id="10541615"/>
    <dbReference type="KEGG" id="pgr:PGTG_13245"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13245"/>
    <dbReference type="HOGENOM" id="CLU_054434_0_1_1"/>
    <dbReference type="InParanoid" id="E3KRD8"/>
    <dbReference type="OrthoDB" id="2508351at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP86863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="255" mass="28277" checksum="C8AE54C11DC3EF57" modified="2011-01-11" version="1">MPLRTLNNPSHLIPTIPTLDGNSVTFTAWRSRLLDVLSIMNVLDIVDKSLLRSSDTKESKLSGRSPDQKGYNPETLVRTGTLVGSRSVNDQVTLSVDLSTIKLTLSVDLAIRYGDVKPASKLFSTIVDAYEKNTRARRVQLKDNFWLARHDPNMPIAKWIARIRKAASDLNTAKIAPADQQVCDRLLRGLDDSWKTIRDHLVYSPKEVSLDDAIGALEAHELSTTAPMDHLGHKGETLASVAKTKRMGCYNCGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KRG5</accession>
    <name>E3KRG5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12631</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178303">
        <property type="protein sequence ID" value="EFP86890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331309.1">
        <property type="nucleotide sequence ID" value="XM_003331261.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KRG5"/>
    <dbReference type="GeneID" id="10541642"/>
    <dbReference type="KEGG" id="pgr:PGTG_12631"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12631"/>
    <dbReference type="HOGENOM" id="CLU_1230416_0_0_1"/>
    <dbReference type="InParanoid" id="E3KRG5"/>
    <dbReference type="OrthoDB" id="2499013at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="207" mass="23159" checksum="ED34366973785557" modified="2011-01-11" version="1">MEILGIKATSTVPTRPSSLYIFPIWLIFVSTSNSILNLLLFQVLNRLKFRFEVPKNLCTRFIMLAPQTHFIAGLLCQLDLGLFMMKKDMSYIAIDLCLGNLHLFSLLVAMNLTQVGRTDGSDIAASGSRKSRRFTISDLNIEIPMNPLSARFSRTRLSSDWAETFLHGTETVGCKDKSERTIRGPSSSSSSFTATHSNDLKAFATRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>E3KRH1</accession>
    <name>E3KRH1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Pre-mRNA-splicing factor Syf1-like N-terminal HAT-repeats domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_12637</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in pre-mRNA splicing and cell cycle progression. Required for the spliceosome assembly and initiation of the DNA replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Associated with the spliceosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the crooked-neck family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178303">
        <property type="protein sequence ID" value="EFP86896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331315.1">
        <property type="nucleotide sequence ID" value="XM_003331267.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KRH1"/>
    <dbReference type="FunCoup" id="E3KRH1">
        <property type="interactions" value="774"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KRH1"/>
    <dbReference type="GeneID" id="10541648"/>
    <dbReference type="KEGG" id="pgr:PGTG_12637"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12637"/>
    <dbReference type="eggNOG" id="KOG1915">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011554_1_0_1"/>
    <dbReference type="InParanoid" id="E3KRH1"/>
    <dbReference type="OMA" id="HIKVWIS"/>
    <dbReference type="OrthoDB" id="541719at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000974">
        <property type="term" value="C:Prp19 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071007">
        <property type="term" value="C:U2-type catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000245">
        <property type="term" value="P:spliceosomal complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000048">
        <property type="entry name" value="Cell cycle control protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000491">
        <property type="entry name" value="Crooked neck-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055433">
        <property type="entry name" value="HAT_Syf1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045075">
        <property type="entry name" value="Syf1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246:SF3">
        <property type="entry name" value="CROOKED NECK-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11246">
        <property type="entry name" value="PRE-MRNA SPLICING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23233">
        <property type="entry name" value="HAT_Syf1_CNRKL1_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="14"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="10" id="KW-0802">TPR repeat</keyword>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1-like N-terminal HAT-repeats" evidence="12">
        <location>
            <begin position="59"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="10">
        <location>
            <begin position="71"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="Pre-mRNA-splicing factor Syf1-like N-terminal HAT-repeats" evidence="12">
        <location>
            <begin position="311"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="578"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="712"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="578"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="745"/>
            <end position="754"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF23233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP86896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="754" mass="89813" checksum="6D4ACD4F5AD4F683" modified="2011-01-11" version="1">MADHRAPKVKNRAAAPVQITAEQLLREAQERQETGHSAPRQKVEDFEELHEYRGRKRKEFEEVIRRTRQDLRAWTKYATWEASQNEFPRARSVFERALDVAPTAEKLWLTYCEMELKARNIQHARNLFDRAVTLLPRINQIWYKYVYLEELLGNIAGARQVFERWMAWEPDEKAWSAYIKMEARYQEHDRASQLYERMIACHPDPKNWIKWAKFEEDRQKIDRAREIFQMAFEYFGEEEEDLERAQSIYTAFAKMESRHKEYDRARTIYKYALDRLPRSKSVGLYASYTNFEKQFGDRAGIESTVLGKRRIQYEEELAHSSQLNYDVWFEYARLEESALKSCDHDSPEKAVERIREVYERAVAQVPPSAEKRYWRRYIFLWLGYATFEEIETKDYDRVRQVYEACLKLIPHKKFTFSKVWEMYAHFELRRLNLDKARKILGTAIGLAPKPKSFKVYLDMELQLREFDRCRKLYEKFLEFDPTYPSAWIQFAGLERGLMETERARAIYEMAIAQPDLYDPECVWKAYIDFEEEEEEWDRARTLFERLALASGHVKVWTSWAKFEMSTGKSISDRYLGLDAEDEEESPDEPIEKDEVAAQQARTEGIRLARQVLQRGYEDIRKRWKNEPEGPVRDNLKEQRSIILDHWKLLETEIGDEVALSKVEAMMPKPMRRWRKLDDTGDKEEYWDLVFPDDEKERNPGTFKLLQLAHAWKAKQQEQQPNNPPQTEPAKSPPKIQIDLPAEDVAQQNAQPIVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3KS43</accession>
    <name>E3KS43_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13337</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EFP87118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331537.1">
        <property type="nucleotide sequence ID" value="XM_003331489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KS43"/>
    <dbReference type="GeneID" id="10542361"/>
    <dbReference type="KEGG" id="pgr:PGTG_13337"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13337"/>
    <dbReference type="HOGENOM" id="CLU_154042_0_0_1"/>
    <dbReference type="InParanoid" id="E3KS43"/>
    <dbReference type="OMA" id="IQPANCL"/>
    <dbReference type="OrthoDB" id="2507429at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125">
        <property type="entry name" value="F21J9.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125:SF3">
        <property type="entry name" value="NO-APICAL-MERISTEM-ASSOCIATED CARBOXY-TERMINAL DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="150" mass="17040" checksum="BCC6EE4C0672A052" modified="2011-01-11" version="1">MQRRPICPVDLTTNEKDDNGDQNNDNDNANPENKGKKKAPNYTEPKDYELCRAWVQVPKDPAVGTNQDGGKFWQQISTVYHEAVPRPIWPIASLKKQWSNNLQPPINKFCGIVHQVEGLNQGGASTEDQLNQALRLYTLDQQTPFKHLKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>E3KS48</accession>
    <name>E3KS48_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_13342</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EFP87123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331542.1">
        <property type="nucleotide sequence ID" value="XM_003331494.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KS48"/>
    <dbReference type="STRING" id="418459.E3KS48"/>
    <dbReference type="GeneID" id="10542366"/>
    <dbReference type="KEGG" id="pgr:PGTG_13342"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13342"/>
    <dbReference type="eggNOG" id="ENOG502S88Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012390_7_0_1"/>
    <dbReference type="InParanoid" id="E3KS48"/>
    <dbReference type="OMA" id="HENKEFE"/>
    <dbReference type="OrthoDB" id="2497879at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125">
        <property type="entry name" value="F21J9.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125:SF3">
        <property type="entry name" value="NO-APICAL-MERISTEM-ASSOCIATED CARBOXY-TERMINAL DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="2">
        <location>
            <begin position="176"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP87123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="310" mass="35522" checksum="F9013D5B9B3CDFA0" modified="2011-01-11" version="1">MLDPMLQQLATPPAQESSTLPVDPLQIQSKRPRASPIEETNRKNDKKQKKEDNTPKNPHWRIEEDKHLCISWLNTSKDADIGTGQKLSAFWERIHEYFIKLNDSYTKTHKNDKNFKPLPNRAVGALECRWATVLHRVNKYCAAYGQVERRLGSGKTRDEIAVEAKELFKADQGINFTLDHCWAILHNSPKWQETMDEIEAKPKKDKKKLQLPTSEASSVKTTTDDESLDVECFGSDRPEGRKAAKKRKYEENEALEGQKELIKISQQKMELMQTVADEAIMGRDLSNLDDISLAYYAAKKKDIAKRNGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3KUN6</accession>
    <name>E3KUN6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ubiquinone biosynthesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178310">
        <property type="protein sequence ID" value="EFP87986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332405.1">
        <property type="nucleotide sequence ID" value="XM_003332357.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KUN6"/>
    <dbReference type="GeneID" id="10535045"/>
    <dbReference type="KEGG" id="pgr:PGTG_13790"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13790"/>
    <dbReference type="eggNOG" id="ENOG502S760">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_858254_0_0_1"/>
    <dbReference type="InParanoid" id="E3KUN6"/>
    <dbReference type="OMA" id="RHNIHIA"/>
    <dbReference type="OrthoDB" id="619536at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="146"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="349" mass="38346" checksum="910A8D0C071BDDC0" modified="2011-01-11" version="1">MHIRHKCTCLAVSGVQQLGTNSRKRMLCRCRRQAGRLVSHNLRPSAALPFPGLPSYPSASSTDAAVVLRRNSSDAPSSTHATANPWKLETHITAEDDLPDQLLTAALPFVRTHGFSAATMMAGVASRPELQRLAISRWTLSGLFPSTTTTSTTTNSNGIGRETHAATEPIGPSKALAERWIQEGNSHMKTRLKNEHLGFKKDRLAGVRRAFEIRLEYNRTIPKEHLLKALALVVTPKHPFFGLPLPLELPHFLPYGSHALAVATDALAGLKDYSKAREWMLRRIRLAGVYSAIELMSIGTDQSPVSLSNELQNLLDASESLATHTANTVEFISYVHRSQRSIIQSLGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KUZ5</accession>
    <name>E3KUZ5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_12542</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178311">
        <property type="protein sequence ID" value="EFP88095.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332514.2">
        <property type="nucleotide sequence ID" value="XM_003332466.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KUZ5"/>
    <dbReference type="STRING" id="418459.E3KUZ5"/>
    <dbReference type="GeneID" id="10535237"/>
    <dbReference type="KEGG" id="pgr:PGTG_12542"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12542"/>
    <dbReference type="eggNOG" id="ENOG502S6K1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008138_2_0_1"/>
    <dbReference type="InParanoid" id="E3KUZ5"/>
    <dbReference type="OrthoDB" id="107110at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266">
        <property type="entry name" value="CHROMOSOME 15, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266:SF1">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP88095.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="846" mass="95266" checksum="B46DAE52B3EABE57" modified="2012-04-18" version="2">MEPEHRKRPFSELESSEPTERAFERFAQFVPPHIDPKTTFELFLNKAGLELVDKYLDDLNNFSHQVPANVHFSSFCSIWHMFVARECSVKSMANTFQIQDNEDLKDQAVRLAFLRNHPNYFTDKTITVSNMIEALLQSSNEAAEQYISSLATKPDSEAITSRISLDIIKKGYDGAYMKSELIVLPILKKLRKQAASWIETAYHSPYTSLVAPSTSGKTRLLTELSKHVCVVYICLRDKDSTGQPPRSALASAMTPTTVVDFSNYYENLLIAIFEVVTDFFRGSRPDKGKVLKNWFDYHCPKNLEPEQPTQFSRDVLNKLNDCYSLIQSGKSKKASTRLKVAVNDMFTQTKFIDSSLTVLLAIDEARSLLLTPKQSSKEFSFFRNFRRALQHIPTNQGFFAILADTTSQVANFNPAPKNDPSARDEIDAGDWLFDPIYQISTLDVLVSPNLPRSWKELVSAARLVNYGSPVFGTYWRVAEAEGMEPLEIFDHIVRLAQSKLLCSTKQPLPAKLTKYQVLALLGPNIQPRLNGTLHLNSELVSSHAAHCNYISPGRDLIISNYPSQFTLACAANKILAGEEILIRCIKELTTILRLGLDAKGTAGELASRIILSCAMRNAMKLKEDLDEIPYGCSVRLADFLKALTGKPEEELQLGQNLPAKTRKELLGNGMVFWNHFTQIYYTPNSNELLHFLYRGLAVQCKPLQPGFDQLFTIYLKKGPDRLDEQNVTFCGVQVKNTLTQPSLVADNHKWTAQYSGIQITKGNPYLVLFMSLKTEGKTAPLPKGDKLRASQVFHGLKSFDCLTEEVVKALQEMIDVEPNIPSLHDDDDGERYANLINPLVYTRPEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVV5</accession>
    <name>E3KVV5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178314">
        <property type="protein sequence ID" value="EFP88430.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332849.2">
        <property type="nucleotide sequence ID" value="XM_003332801.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KVV5"/>
    <dbReference type="GeneID" id="10540732"/>
    <dbReference type="KEGG" id="pgr:PGTG_14008"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14008"/>
    <dbReference type="HOGENOM" id="CLU_047292_0_0_1"/>
    <dbReference type="InParanoid" id="E3KVV5"/>
    <dbReference type="OrthoDB" id="2511516at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88430.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="227" mass="24753" checksum="F1186558A47358BC" modified="2012-04-18" version="2">MIVDLGESTKFTQSMCEKTSVIGLGIVLTKKEINGPDTTSSSGSKILQVIMQHTDYDPVAKAQVEFKTMYHIGGRKNLANTFGLFQLSREILISGNIVGYTEQDYMWIVAAISVSVTSGHQSNVSQLSRPSERPQPYTRRPGLILIEDSSPAEGSSLSPNNYGIGPSDRDNMAEPEPFPNLSTDLPDVSNDNYYESIPSTTGKKRTKRAILADAKRAAKASTSLTPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KW40</accession>
    <name>E3KW40_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178314">
        <property type="protein sequence ID" value="EFP88515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332934.1">
        <property type="nucleotide sequence ID" value="XM_003332886.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KW40"/>
    <dbReference type="GeneID" id="10540979"/>
    <dbReference type="KEGG" id="pgr:PGTG_14093"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14093"/>
    <dbReference type="HOGENOM" id="CLU_1769043_0_0_1"/>
    <dbReference type="InParanoid" id="E3KW40"/>
    <dbReference type="OrthoDB" id="10628470at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="147" mass="17207" checksum="8507FD194C4B27D2" modified="2011-01-11" version="1">MADSQMNLQENAELIQRYLLHLRLRKEEADRSYIPTPWEELSEEEQLLLATYHAHIHQEEDLDQQIKFRGFLREIREKNTQLCRKRYLDKMSTFETGVIRQERASPTPSIDTDNGSSDPTDKSPTTQTYEAPSQLQRDLAAAKELQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>E3KXI1</accession>
    <name>E3KXI1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="22">Glutamate synthase [NADH]</fullName>
            <ecNumber evidence="19">1.4.1.14</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="27" type="ORF">PGTG_15142</name>
    </gene>
    <organism evidence="27 28">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="28" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="28">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="21">Forms L-glutamate from L-glutamine and 2-oxoglutarate. Represents an alternative pathway to L-glutamate dehydrogenase for the biosynthesis of L-glutamate. Participates with glutamine synthetase in ammonia assimilation processes. The enzyme is specific for NADH, L-glutamine and 2-oxoglutarate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="20">
            <text>2 L-glutamate + NAD(+) = L-glutamine + 2-oxoglutarate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13753"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="1.4.1.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="24">
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </cofactor>
        <text evidence="24">Binds 1 [3Fe-4S] cluster.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-glutamate biosynthesis via GLT pathway; L-glutamate from 2-oxoglutarate and L-glutamine (NAD(+) route): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Energy metabolism; nitrogen metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Nitrogen metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the glutamate synthase family.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.14" evidence="19"/>
    <dbReference type="EMBL" id="DS178318">
        <property type="protein sequence ID" value="EFP88939.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333358.2">
        <property type="nucleotide sequence ID" value="XM_003333310.2"/>
    </dbReference>
    <dbReference type="FunCoup" id="E3KXI1">
        <property type="interactions" value="164"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KXI1"/>
    <dbReference type="GeneID" id="10542219"/>
    <dbReference type="KEGG" id="pgr:PGTG_15142"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15142"/>
    <dbReference type="eggNOG" id="KOG0399">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000422_8_2_1"/>
    <dbReference type="InParanoid" id="E3KXI1"/>
    <dbReference type="OrthoDB" id="4327079at2759"/>
    <dbReference type="UniPathway" id="UPA00045"/>
    <dbReference type="UniPathway" id="UPA00634">
        <property type="reaction ID" value="UER00690"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051538">
        <property type="term" value="F:3 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016040">
        <property type="term" value="F:glutamate synthase (NADH) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016639">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019676">
        <property type="term" value="P:ammonia assimilation cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097054">
        <property type="term" value="P:L-glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00982">
        <property type="entry name" value="gltB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00713">
        <property type="entry name" value="GltS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02808">
        <property type="entry name" value="GltS_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1060.10:FF:000006">
        <property type="entry name" value="Glutamate synthase (NADPH/NADH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000031">
        <property type="entry name" value="Glutamate synthase 1 [NADH]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000017">
        <property type="entry name" value="Glutamate synthase [NADH], amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000113">
        <property type="entry name" value="Glutamate synthase [NADH], amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000022">
        <property type="entry name" value="Glutamate synthase [NADH], amyloplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.60:FF:000001">
        <property type="entry name" value="Glutamate synthase, large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000001">
        <property type="entry name" value="Glutamate synthase, large subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.60">
        <property type="entry name" value="Glutamate synthase, alpha subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028261">
        <property type="entry name" value="DPD_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002489">
        <property type="entry name" value="Glu_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036485">
        <property type="entry name" value="Glu_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006982">
        <property type="entry name" value="Glu_synth_centr_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012220">
        <property type="entry name" value="Glu_synth_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002932">
        <property type="entry name" value="Glu_synthdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006005">
        <property type="entry name" value="Glut_synth_ssu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051394">
        <property type="entry name" value="Glutamate_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01317">
        <property type="entry name" value="GOGAT_sm_gam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008730">
        <property type="entry name" value="PRK11750.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43100">
        <property type="entry name" value="GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43100:SF1">
        <property type="entry name" value="GLUTAMATE SYNTHASE [NADPH] SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14691">
        <property type="entry name" value="Fer4_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00310">
        <property type="entry name" value="GATase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04898">
        <property type="entry name" value="Glu_syn_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01645">
        <property type="entry name" value="Glu_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01493">
        <property type="entry name" value="GXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000187">
        <property type="entry name" value="GOGAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69336">
        <property type="entry name" value="Alpha subunit of glutamate synthase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51971">
        <property type="entry name" value="Nucleotide-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="18 24" id="KW-0003">3Fe-4S</keyword>
    <keyword evidence="8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0274">FAD</keyword>
    <keyword evidence="9" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-0288">FMN</keyword>
    <keyword evidence="17" id="KW-0314">Glutamate biosynthesis</keyword>
    <keyword evidence="13" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="15" id="KW-0408">Iron</keyword>
    <keyword evidence="16 24" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="28" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="26">
        <location>
            <begin position="50"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="25">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="25">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="23">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1173"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1179"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="24">
        <location>
            <position position="1184"/>
        </location>
        <ligand>
            <name>[3Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:21137"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00024383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00048867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00057049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00068518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="PIRSR" id="PIRSR000187-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="PIRSR" id="PIRSR000187-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="26">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="27">
        <source>
            <dbReference type="EMBL" id="EFP88939.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="28">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="2131" mass="233974" checksum="BD8873B46637AE21" modified="2012-04-18" version="2">MAPKVHNNQEQKEIKSSEPDSNLIRSSNHWAGALPAPQGLYDPDQEKDSCGVGFVCHIKGQPNHKIVSDARSLLCNMTHRGATGADARDGDGAGVMTGIPHAFFLREASLLSIDLPAQGRYAVGNVFFRPNPPEALIEHKATFERIALTHKLKVLGWRELPRNNSILGPAALSREPIILQPFVVPDSNEPDAYFDEKEFERQLYVLRKHASHAISLADWFYICSLSNKVIIYKGQLSPSQVYEYYYDLNHVLFESHFCLVHSRFSTNTFPSWDRAQPLRWAAHNGEVNTVRGNKNWMRAREGNLSSDKFGDQLESLYPIIEEGGSDSAAFDNVLELLVVNGVMSLPEAIMMLVPEAWQNNPNMDPAKSAFYQWAACVMEPWDGPALFTFSDGRYCGANLDRNGLRPCRWVTTDEDLIICASEVGAITIAPETITRKGRLQPGRMLLVDTQEGRIVDDKELKMATAQKKPFREWINNQMLRLPDILKHNKSISSTLLSDTLDETRISEDPRLLAFGYTLEQLDLLMRPMVSDGKEALGSMGNDAPLACLATQPRLIYDYFRQLFAQVTNPPIDPIRESIVMSLECYVGPEGNLLALDESQAGRLALPSPILSVQEFRALQDLHTFNSAWSSHTIDITFEKRSGLPGYVVTLDRICQAASAAVQAGCRVLALSDRAVGAGRVAVSALAAIGAVHHHLIKSKERSKVALLVETGEAREVHHLCVLLGYGADGICPYLAMEAILKLRREGLVKVDLTDVDLIENFRHATNNGILKVMSKMGISTLQSYKGAQIFEALGLHQTVVERCFVGTASRIQGTTFELLALDTFEFHERGFPSRQVVQPPGLPESGEYHWRDGGEAHINDPVSIANLQDAVRSKNQSAYDVYSQNAQKQVRAVTLRGLLDFDYESAQSIPLEQVEPWHELVKRFCTGAMSYGSISQEAHSALAIAMNRLGGKSNTGKGGEDGSRSLIMPNGDTMRSAIKQVASGRFGVTSNYLADSDELQIKMAQAAKPGEGGELPGHKVSELIAKTRHLTAGVGLISPPPHHDIYSIEDLKQLIYDLKCANPRARVSVKLVSEVGVGIVASGVAKAKADHILISGHDGGTGASRWTGIKYAGLPWELGLAETHQTLVLNDLRGRVCLQTDGQIRTGRDVAIAALLGAEEFGFATTPLIAMGCIMMRRCHQNTCPVGVATQDPVLRAKFTGQPEHVINFFYYVAEELRTHMAKLGFRTLNEMVGRTDLLKVDETLRNPKTVNIDLSAVLKPAWKMRPGVATFKTKQQDHKMYTRLDNKFIDEAEPALAKGLPVRIEADVKNTDRALGTSLANRVSKAYGEQGLERDIIHVDLRGSAGQSLGAFLAPGITLELEGDANDYVGKGLSGGRLIVYPPRSSPFKAEENVIVGNVCLYGATSGEAFFRGIAAERFAVRNSGAIAVVEGVGDHGCEYMTGGRVVVLGSTGRNFAAGMSGGIAYVLDMAREFKNKVNSEMVELGTVNDPHEIAELRGMIEDHRHFTKSEQAARILRNFNEFLPRFVRVMPLDYKAVLEAGAKAAIQSDPHKNMPGFDIKGDAFNPAEDKAKTNVNDQVEIPSATLGHSEPPVVDLEDSMVDAESAKKIVEKLDKTKGFMKYKRLNENYRNPKSRTKDWAELSTRLTESQLKVQAARCMDCGVPFCQSDTGCPISNVIPKWNDLVFKGQWLDALNRLLMTNNFPEFTGRICPAPCEGACVLGINEAPVGIKSIECAIIDKGFEMGWIVPRPPVERTGKKVAVIGSGPAGLACADQLNRAGHMVTVYDRNDRHGGLLMYGIPNMKLDKKVVQRRLDLMAAEGITFVANAHVGVSTDVNQIRADNDALVVSTGATWPRNLNLPNRNLDGIHFAMEFLQLNTKSLLDSELSDSSYLSAKGKNVIVIGGGDTGNDCIGTSLRHGAKSVTNFELLPQPPQSRASDNPWPQWPKVFRVDYGHTEVQNFFGKDPREYCISTKEFVSDGEGKLKGLNTVRVEWTKDSRGAWKMKEIAGSEEFYECELCLLALGFLGPEESMIKSLGLKQDPRSNILTNLADNLRRFQYQTSIDGVFAAGDCRRGQSLVVWGIQEGRAAAVDVDNFLSGKSASKLPSKGSIKVRDFAVDAFEASVVAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KYB7</accession>
    <name>E3KYB7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_15487</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178321">
        <property type="protein sequence ID" value="EFP89308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333727.1">
        <property type="nucleotide sequence ID" value="XM_003333679.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KYB7"/>
    <dbReference type="GeneID" id="10547028"/>
    <dbReference type="KEGG" id="pgr:PGTG_15487"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15487"/>
    <dbReference type="HOGENOM" id="CLU_081654_0_0_1"/>
    <dbReference type="InParanoid" id="E3KYB7"/>
    <dbReference type="OrthoDB" id="10370066at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172317" evidence="2">
        <location>
            <begin position="27"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP89308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="297" mass="32321" checksum="187D6E17E1A2BB64" modified="2011-01-11" version="1">MYNASKALSALILTVVLSVLVGHSASAPPSSPADAFKKLSSSDPTNLAVPRMNGDADEEAEQARKGSSLVRHRSLDSDLLSNDDGSPPPVAQSANAPSSPNLSITAPSVGSAGGGAIKLLLHQPSPKNSFQPRRFQDDSVSEDPKQFTVAPPPDQNSQRKRHTQSHQSTSYEAGDIRPNSPKNSFQPRRFQDDSVSEDAKQFMVAPPPDQNPQRKRHTESRQTSSYEAVDLSSNSPKNSFQPRRFQDDSVSEDPKQFMVAPPPDQNPQRKRHAESHQTSSNEAVDVSRNKALGFGWH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3KYH0</accession>
    <name>E3KYH0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Prokaryotic-type class I peptide chain release factors domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_15540</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the prokaryotic/mitochondrial release factor family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178321">
        <property type="protein sequence ID" value="EFP89361.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333780.2">
        <property type="nucleotide sequence ID" value="XM_003333732.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KYH0"/>
    <dbReference type="FunCoup" id="E3KYH0">
        <property type="interactions" value="528"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KYH0"/>
    <dbReference type="GeneID" id="10547166"/>
    <dbReference type="KEGG" id="pgr:PGTG_15540"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15540"/>
    <dbReference type="eggNOG" id="KOG2726">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036856_0_3_1"/>
    <dbReference type="InParanoid" id="E3KYH0"/>
    <dbReference type="OrthoDB" id="2019491at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000070">
        <property type="entry name" value="Related to MRF1-peptide chain release factor, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1660">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005139">
        <property type="entry name" value="PCRF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000352">
        <property type="entry name" value="Pep_chain_release_fac_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045853">
        <property type="entry name" value="Pep_chain_release_fac_I_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050057">
        <property type="entry name" value="Prokaryotic/Mito_RF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43804">
        <property type="entry name" value="LD18447P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43804:SF7">
        <property type="entry name" value="LD18447P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03462">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00472">
        <property type="entry name" value="RF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00937">
        <property type="entry name" value="PCRF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75620">
        <property type="entry name" value="Release factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00745">
        <property type="entry name" value="RF_PROK_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0488">Methylation</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prokaryotic-type class I peptide chain release factors" evidence="4">
        <location>
            <begin position="168"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP89361.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="308" mass="34750" checksum="F468D79AAFD3FA87" modified="2012-04-18" version="2">MREIAITELKTIEERALRTIEQVKQGLIQVASPRPESYDQSALVEIKPGVGGVEASHFATLLMNLYTRLATRKRWHIKPVSVEYMTSGQGQDGLREAVLEIEGQAVYGSLRWEAGVHRVQRVPVMQNTASIHTSTASVIVLPLNPSGSEENESEDLFDEKDIKLETMRSQGAGGQHVNKTESAVRLTHIPTGTVVSMQDSRSQHQNRERAYMILRARLLDLKLRQKVIENRESRLSQVKNTNRSEKIRTYNFQQGRVTDHRIGLTLPRLNDIIDGGETLDIIWDGLEKDEEANTIQSIYSTTHTSNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZ45</accession>
    <name>E3KZ45_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 3 subunit G</fullName>
            <shortName evidence="5">eIF3g</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Eukaryotic translation initiation factor 3 RNA-binding subunit</fullName>
            <shortName evidence="5">eIF-3 RNA-binding subunit</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Translation initiation factor eIF3 p33 subunit homolog</fullName>
            <shortName evidence="5">eIF3 p33 homolog</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">TIF35</name>
        <name evidence="9" type="ORF">PGTG_15719</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. This subunit can bind 18S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
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        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
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    <dbReference type="PANTHER" id="PTHR10352">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT G"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF12353">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037949">
        <property type="entry name" value="Transl_init_eIF-3_RNA-bind"/>
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        <property type="entry name" value="RRM"/>
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    </dbReference>
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        <property type="entry name" value="RNA-binding domain, RBD"/>
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    </dbReference>
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    <proteinExistence type="inferred from homology"/>
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        <location>
            <begin position="214"/>
            <end position="299"/>
        </location>
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        <location>
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        <location>
            <begin position="160"/>
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        </location>
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            <begin position="169"/>
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        <location>
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        </location>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
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    </evidence>
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        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
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    </evidence>
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        <source>
            <dbReference type="HAMAP-Rule" id="MF_03006"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50102"/>
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        <source>
            <dbReference type="EMBL" id="EFP89570.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="299" mass="32595" checksum="50471BCD093F8DF0" modified="2011-01-11" version="1">MATLAPPQLSAPATKTSWADELDEDELPPSTEKIDPVTGIKTIVEYKWDETEEKKIKTVRKIKRTLVTTRVSPTVAARKGWAKFGLEKGKKSGPDLATTTLGEAIKLKIAVPGLIKEPVVEQTTKATIVPGGKRITCRLCQGDHFTASCPYKDTLGGVLGGDAADQDQQDGPGASQAQTNNAAGGGTGKYVPPSQRDGAKRGESMQKSNRDDLPTLRVTNLSEDVQESDLWDLFGRFGRISRIYVGKDQETGLCKGFAFVSFEDRMEAERAMKKINGLPYDHLILGCMWSLPRGEREAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZ68</accession>
    <name>E3KZ68_PUCGT</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15742</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15742"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="135"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="115"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89593.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="135" mass="15076" checksum="F987B39F52E4DB92" modified="2012-04-18" version="2">MQISSIFCAFHLLNCYIITAHPNLYIKTLGKREIDDVTAGTQLHFLHKRMEKSSQDLAKEAKADMSSKQGNLTNDQWISMVRGLVAVQSSFIEIENKIGDIEEKLGKMRGNIDTSDARLDGSSHTNDKSYKASGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3L0G2</accession>
    <name>E3L0G2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_15908</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178328">
        <property type="protein sequence ID" value="EFP90060.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334479.2">
        <property type="nucleotide sequence ID" value="XM_003334431.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L0G2"/>
    <dbReference type="GeneID" id="10546038"/>
    <dbReference type="KEGG" id="pgr:PGTG_15908"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15908"/>
    <dbReference type="HOGENOM" id="CLU_004591_5_2_1"/>
    <dbReference type="InParanoid" id="E3L0G2"/>
    <dbReference type="OrthoDB" id="2507436at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP90060.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="398" mass="46170" checksum="C2E4FE7B3932CDE0" modified="2012-04-18" version="2">MPPDKYFFQEGSNQFSCILCRGKSTSNQKKHARGQRHLNLVRLDEERIERQQERINVNETLNQQESNRPRIDIEPICDDPVCSDEQVEPLVNETDDESDVVPISLFEDGESEHEEEELDWLNLLGVAIDQIDDEPADSFDGLLVPAFQRDLAEIEEIMTVDSAWFPFVNKDYLIGSLFVGYLHKIISRDLYHQIQSILTLHHIHLPGWETLRRTRQKIRLMSHHSLVEKESLFGQPMFALDAREIISDDLRNPLVAPHLDFLPEDAHGKGIFKTSQSAKWLKHLAPDIRVQMVAYNKKHYYTYEPVQLKNDDIIIPIFFYTLQSNTFAKCYKPQITSNRHDSTVKIAIASDILYDDPDLQEINLDDFDLLYSEIISSDGLKLLEYCGNLLYGKKYALY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3L1F6</accession>
    <name>E3L1F6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178332">
        <property type="protein sequence ID" value="EFP90381.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334800.2">
        <property type="nucleotide sequence ID" value="XM_003334752.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L1F6"/>
    <dbReference type="GeneID" id="10530275"/>
    <dbReference type="KEGG" id="pgr:PGTG_16141"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16141"/>
    <dbReference type="HOGENOM" id="CLU_752564_0_0_1"/>
    <dbReference type="InParanoid" id="E3L1F6"/>
    <dbReference type="OrthoDB" id="10320957at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174079" evidence="1">
        <location>
            <begin position="26"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90381.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="348" mass="39359" checksum="BE76A368500CF0D3" modified="2012-04-18" version="2">MVYLAMLQLRSFQFQLLWCSVLVLAPRPDIDLNLMPEETSEEALSFTEPATPPIPSAAESLLGKRKFHSVQLSSLSPPEPRACLSYADSGSISTDLIKPRFAAESAFTPDHSGFQDSFVAVDQVVGNRIRRLLPASDYKQGFAHDPQSSRSSYRTSLELSNNNVAGFLSNNQVIEELLSKFSKKFRETIHSEDYHSSRSVSIDKHPHIPIARFYRANGPGKLAKVLHHKHLRQQINRRLLTMYNRLLISSFKINGALLTLLNIPILNRRKQQQRFFEWLEKEIFEPTSGVPVLGFAARPDLHWGSGAPEVQVGNPQLELIKYFSEENCDPNSCASSLLKLYRTQHQSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3L308</accession>
    <name>E3L308_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17205</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178339">
        <property type="protein sequence ID" value="EFP90933.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335352.2">
        <property type="nucleotide sequence ID" value="XM_003335304.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L308"/>
    <dbReference type="GeneID" id="10528347"/>
    <dbReference type="KEGG" id="pgr:PGTG_17205"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17205"/>
    <dbReference type="HOGENOM" id="CLU_052708_0_0_1"/>
    <dbReference type="InParanoid" id="E3L308"/>
    <dbReference type="OrthoDB" id="2506642at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="218"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90933.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="277" mass="30707" checksum="34AA9E548EDBDFE7" modified="2012-04-18" version="2">MLFISTSNPSQGGTKRYSIPPGIFNFLLIGFPALTAVASIFLIVHKASLVQKQDVSYNVLINLLTDAAKLWDGGEKILSESRNFLLQATYEVFSLDTFLKNRSRIRIGLFWAFMAIPSILFYVGGVIALVTTVYQPIKTPKKLQPANLNPNEIKTVGNEMENGPIILGKDGSRKLSRSLKFLWLHYFSMSMALSMDAATSMVFYLNKDNLKDIRLARKFFVTNLSSSIFTLVAMIILLARIIGDGKEAKNSASNCTSGFTESNYSASKVAPGNKQYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3L435</accession>
    <name>E3L435_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_17167</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178344">
        <property type="protein sequence ID" value="EFP91310.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335729.2">
        <property type="nucleotide sequence ID" value="XM_003335681.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L435"/>
    <dbReference type="STRING" id="418459.E3L435"/>
    <dbReference type="GeneID" id="10529140"/>
    <dbReference type="KEGG" id="pgr:PGTG_17167"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17167"/>
    <dbReference type="eggNOG" id="KOG0812">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_476619_0_0_1"/>
    <dbReference type="InParanoid" id="E3L435"/>
    <dbReference type="OrthoDB" id="2505607at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="entry name" value="HEPARAN-ALPHA-GLUCOSAMINIDE N-ACETYLTRANSFERASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40407">
        <property type="entry name" value="MEMBRANE PROTEIN-LIKE PROTEIN"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="110"/>
            <end position="128"/>
        </location>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="158"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="201"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="304"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="364"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="406"/>
            <end position="430"/>
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    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="465"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="497"/>
            <end position="517"/>
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    <feature type="transmembrane region" description="Helical" evidence="2">
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            <begin position="532"/>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP91310.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="567" mass="65271" checksum="78D91C1094CB7011" modified="2012-04-18" version="2">MNQPASSNETSHLLHQQATRDQNQNNQRPTSVRFFIPTQPHSTITNFILNYSHSQEGLLSSVALNENDLSVDPVGLDLDLHHSALNSNQLPLPCFNPLFKKSRFLMTLDIFKAIILSIQTIYTANQFIGPYSNSNQPFTPYWHQHIKFPLSHPSQYTIHLLTLICSPALHFVLGSSAVLAIHTRKCLGWRSHQIFFRSLKLGLSLLVLNQLLMSQTIFQTRGQVFLTTLPIWSLGWNLVMITTILIGLFLAERAILNYLYTKTRGSLLEEPIRRRPEENETDEDQDEDVKTTEWMRGWADRIRWAIDAFLLIGSGLLPLLIAYMLPSDREDPDPDWWIESSLTRSYWFWFFFTPTPQMSAYPRFGMVSNFAPIGWLPFVLLGAFYGRCLIRKHQERIQTIHRHLKLAIISISVFGLTRIIDIGNLGVPYITDDHDPARAVTGRWFRWLRGSWEMAFYTTVYPPDLGHVSLASTLIFLLLALLDLLPQALLTFNPLLIFGRAPIFFYSIQLLLFHYLLPSVLVILDWPRNLNLFWISVVSFSSLPVLWLACEAWVIFKDCKSPQSVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3L451</accession>
    <name>E3L451_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16980</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP91326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="STRING" id="418459.E3L451"/>
    <dbReference type="GeneID" id="10529156"/>
    <dbReference type="KEGG" id="pgr:PGTG_16980"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16980"/>
    <dbReference type="HOGENOM" id="CLU_1321469_0_0_1"/>
    <dbReference type="InParanoid" id="E3L451"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="208" mass="22305" checksum="B3F5CC55A33452B7" modified="2011-01-11" version="1">MGNPPGFIFFTSTASCPTSGLSQLQIPLSALSSDSRHADCGEYAVSSGKPARCKPRVGRFSHRGPDEEPALGATPTADGHVGPALEVGPTAAPDTSRSEPLQEIWAGLGGSDQSIIDLEGARDGFEDIHQENQINQTHRILISDLVCQLTLEYIAAILKEPFDQLDHLRPEGKLHRKLKAADVLLSSTGEMSPEVIKQSGYDSQADIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3L4J3</accession>
    <name>E3L4J3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Superoxide dismutase copper/zinc binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17718</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178347">
        <property type="protein sequence ID" value="EFP91468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335887.1">
        <property type="nucleotide sequence ID" value="XM_003335839.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L4J3"/>
    <dbReference type="GeneID" id="10529469"/>
    <dbReference type="KEGG" id="pgr:PGTG_17718"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17718"/>
    <dbReference type="HOGENOM" id="CLU_1361004_0_0_1"/>
    <dbReference type="InParanoid" id="E3L4J3"/>
    <dbReference type="OrthoDB" id="2495266at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006801">
        <property type="term" value="P:superoxide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174020" description="Superoxide dismutase copper/zinc binding domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="174" mass="18795" checksum="0865F98E30D0DB27" modified="2011-01-11" version="1">MSTKMYLFMIVVCTITLTSILAADNPPPQKNRYPHTASAKINNGAVQVTIDFAKVPKENRPPETSHFKADRPLTSVTVKYSGLEAGKDFTYFTDPECDHLVQFTRNRLQDLSGKHGVLKGNGPAETPLAVYYDAHLPLTYSQRGIVGKSVVIHDPTGAAVACGNVVDPTAPAVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3L5G2</accession>
    <name>E3L5G2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17787</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
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                <person name="Grabherr M.G."/>
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                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
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                <person name="Shapiro H."/>
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                <person name="Tuskan G.A."/>
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                <person name="Ellis J.G."/>
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                <person name="Zhong S."/>
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                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
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                <person name="DeCaprio D."/>
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                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
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                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
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                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
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                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
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                <person name="Joly D.L."/>
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                <person name="Cantarel B.L."/>
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                <person name="Feau N."/>
                <person name="Field M."/>
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                <person name="Gelhaye E."/>
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                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
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                <person name="Morin E."/>
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                <person name="Pangilinan J.L."/>
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                <person name="Pearson M."/>
                <person name="Quesneville H."/>
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                <person name="Sakthikumar S."/>
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                <person name="Tuskan G.A."/>
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                <person name="Ellis J.G."/>
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                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
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                <person name="Cuomo C.A."/>
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                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
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                <person name="Kuees U."/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173143" evidence="2">
        <location>
            <begin position="21"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP91912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="220" mass="23429" checksum="2A8BD972E045FFCC" modified="2011-01-11" version="1">MAGLRTLLSVLLVWIHNCDCVHDAKDPDPVSTSPNCSPSIRPETNVSTPIKAWISGVCATEACTDESLKEALAELSKGCAAELTNKSPHAGALFSILSHYKDIRSTSCKDTEKLDFCQPDLVGSVEKLEKTGRTFFTVSAQAYCTECDKKSASEKPKSSGKKGGAEKPNPPTKRSPTKKSSLHICSGSNLDDKRVEVGLPGPQKKTDAPASPPATPDESN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3L6M2</accession>
    <name>E3L6M2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18094</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178360">
        <property type="protein sequence ID" value="EFP92197.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336616.2">
        <property type="nucleotide sequence ID" value="XM_003336568.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L6M2"/>
    <dbReference type="STRING" id="418459.E3L6M2"/>
    <dbReference type="GeneID" id="10537525"/>
    <dbReference type="KEGG" id="pgr:PGTG_18094"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18094"/>
    <dbReference type="HOGENOM" id="CLU_018307_2_0_1"/>
    <dbReference type="InParanoid" id="E3L6M2"/>
    <dbReference type="OrthoDB" id="2500663at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="369"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="376"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92197.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="480" mass="53026" checksum="E895CBA0CEFC1BD1" modified="2012-04-18" version="2">MSDANPPPSGQEQPATDSTRDSTRTADGTDMSTAKEWFKAVLKIQHSAISQAQEDCRQALEDRRADRQLFLAAHQASADRIGRLEDLLLAMNIKNEVNARAEHPAPGRVDLQKFRTSDGPTYRGPFQETEPFLRWIHGVQIFFETKSVNNAADKIKILGNLVAETNLQSFYANEASGFLTKSWEEFKTRMFDFALPNNWRSGLQRQIRKLDMSPSETFLEYSTRARTLQSLFNFDAEKFSKLGDLQLAQFAVYGLPDALQDRINERQLLEASPFAYGQFEKKANASFLALHRSPELPPRAKSTPNAPPTLGRDEFIWRVHAFLDSQGLCHFCKKHCGNAAGACPGPIDRSHINIPNNFQTPTKPLDYVAPRAWSKPSTGPGQSSQPPAGRPPARAASVAGIAEVSPLEAHVAGLTVDAAIREDARFDHEFDDEGCFPALGTAAVAALEDLDTQLLQNEIDRATQASDGWTGNLADDIAGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3L7E1</accession>
    <name>E3L7E1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_18291</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178365">
        <property type="protein sequence ID" value="EFP92466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003336837.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L7E1"/>
    <dbReference type="GeneID" id="10538459"/>
    <dbReference type="KEGG" id="pgr:PGTG_18291"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18291"/>
    <dbReference type="HOGENOM" id="CLU_1559021_0_0_1"/>
    <dbReference type="InParanoid" id="E3L7E1"/>
    <dbReference type="OrthoDB" id="2507030at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP92466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="179" mass="20769" checksum="089CDB832311D52F" modified="2011-01-11" version="1">MKLLKNKWISYNHRAINYNATYTPNPDLPTPTFDEVKSFQINNSFWNIGLLDHPNEPWAIDVETQKGITAYLTMTNCDDELRRISREARQALNWAVNMAAKVENILDALLMDVQETDVLTETQQNLQDICTAENLPKSVMESVISNTAKKFCRLWITWNSSCNTVLLWRHCGKNYVERQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8K7</accession>
    <name>E3L8K7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_18800</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178375">
        <property type="protein sequence ID" value="EFP92882.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337301.2">
        <property type="nucleotide sequence ID" value="XM_003337253.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L8K7"/>
    <dbReference type="GeneID" id="10542689"/>
    <dbReference type="KEGG" id="pgr:PGTG_18800"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18800"/>
    <dbReference type="HOGENOM" id="CLU_2832395_0_0_1"/>
    <dbReference type="InParanoid" id="E3L8K7"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP92882.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="66" mass="7159" checksum="D7FFDC8EF8B707C5" modified="2012-04-18" version="2">MGMVDNLVMYTANNLGMDMADHLGMDMADHPGLDIADHPGMDMADHPGMDMVDHPGMDMADHPAYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8X7</accession>
    <name>E3L8X7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4219 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_19015</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178378">
        <property type="protein sequence ID" value="EFP93002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337421.1">
        <property type="nucleotide sequence ID" value="XM_003337373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L8X7"/>
    <dbReference type="GeneID" id="10542978"/>
    <dbReference type="KEGG" id="pgr:PGTG_19015"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19015"/>
    <dbReference type="eggNOG" id="ENOG502SSQ0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051119_1_0_1"/>
    <dbReference type="InParanoid" id="E3L8X7"/>
    <dbReference type="OrthoDB" id="2505270at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027532">
        <property type="entry name" value="Mdm12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204:SF1">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="209"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP93002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="297" mass="33794" checksum="91B95ACD38D340DB" modified="2011-01-11" version="1">MDNMTETSSHKTRFLLTNSNYVIWKISIEAKLFDLGAREYVVGITKVDEKTSAEEIAKLSKLNTKAYSLIIQNLDSENLSLVSTTLPITDQFNGKALWTLLRNKYAGSNLAARSAALDIFLDLEFKDVSLFATAIRLSNQKMVLAGIMLDDQVKMMIMLQKLPRQEFRSFRDIVAMGFATESFEAIVKRLEAYTVTNNIKKDSLDEPQVSPQTSLHTQSVDSSLRSNPVCPHCKKVGHRPNNCWLKFPEKAPNTKRSHMTTDSQLSSNPTDFTWFRTAEGVRHHVDEIKFENVTYFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3L9F8</accession>
    <name>E3L9F8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Concentrative nucleoside transporter N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_19143</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178384">
        <property type="protein sequence ID" value="EFP93183.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337602.2">
        <property type="nucleotide sequence ID" value="XM_003337554.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L9F8"/>
    <dbReference type="GeneID" id="10543333"/>
    <dbReference type="KEGG" id="pgr:PGTG_19143"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19143"/>
    <dbReference type="eggNOG" id="KOG3747">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016813_1_2_1"/>
    <dbReference type="InParanoid" id="E3L9F8"/>
    <dbReference type="OrthoDB" id="6075923at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005337">
        <property type="term" value="F:nucleoside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901642">
        <property type="term" value="P:nucleoside transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008276">
        <property type="entry name" value="C_nuclsd_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011657">
        <property type="entry name" value="CNT_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002668">
        <property type="entry name" value="CNT_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011642">
        <property type="entry name" value="Gate_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10590">
        <property type="entry name" value="SODIUM/NUCLEOSIDE COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10590:SF4">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 28 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07670">
        <property type="entry name" value="Gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07662">
        <property type="entry name" value="Nucleos_tra2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01773">
        <property type="entry name" value="Nucleos_tra2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="16"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="48"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="131"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="165"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="226"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="Concentrative nucleoside transporter N-terminal" evidence="8">
        <location>
            <begin position="52"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoside transporter/FeoB GTPase Gate" evidence="10">
        <location>
            <begin position="135"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="Concentrative nucleoside transporter C-terminal" evidence="9">
        <location>
            <begin position="236"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP93183.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="301" mass="32609" checksum="2B4DC63DF1DB7C38" modified="2012-04-18" version="2">MALIGKPIMKSTNQRVRLLGGWLVLLGLWLASTFGIKLGPGNNYTDRARSLVGVMIFQLGFWASSKNKSLICWRTVIVGLILQQLLALAVLKSKAGYDFFHWIATAATDILDQGTKVGGFFFSPQVVDNHWFFVNTLAAIIFFIALVQLLYYFGIMQAVMVKFAWVFYTLMGVSGVEALAAAASPFVGQGESVILIRPFAAQMTAAEIHQVMTSGFSTIAGSVLKAYVALGIPAVYLISASIMSIPASLAISKLRFPEDSQPLTAGKVTIPEDEQTNRKGSNAMMTFSDGAWLGFKVAGMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3LA81</accession>
    <name>E3LA81_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_19243</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178393">
        <property type="protein sequence ID" value="EFP93440.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337859.2">
        <property type="nucleotide sequence ID" value="XM_003337811.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LA81"/>
    <dbReference type="GeneID" id="10537548"/>
    <dbReference type="KEGG" id="pgr:PGTG_19243"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19243"/>
    <dbReference type="HOGENOM" id="CLU_1939185_0_0_1"/>
    <dbReference type="InParanoid" id="E3LA81"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP93440.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="130" mass="15337" checksum="121EE12DDB855492" modified="2012-04-18" version="2">MAGIFNGWLFIQTHGAPQVTIFDLEDWDSTPEMKETVKQELRDNRWTDATESHCTKKSTIDLAHRRLKIDYSPREHAVCITNYNNEGGPSLHYLLRDGQAERFFRHTISPNEEHIIPFEEGHWLMTWIRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3LAH2</accession>
    <name>E3LAH2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19543</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178397">
        <property type="protein sequence ID" value="EFP93547.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337966.2">
        <property type="nucleotide sequence ID" value="XM_003337918.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LAH2"/>
    <dbReference type="GeneID" id="10543823"/>
    <dbReference type="KEGG" id="pgr:PGTG_19543"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19543"/>
    <dbReference type="HOGENOM" id="CLU_031648_1_0_1"/>
    <dbReference type="InParanoid" id="E3LAH2"/>
    <dbReference type="OrthoDB" id="2496330at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93547.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="604" mass="69152" checksum="8C4D50DB50D5B6F6" modified="2012-04-18" version="2">MAFLVTLPGRPFLYSLFWCLAIVLATNLHFDLNKSPEESSVEELAQLVTHESFPGQSTSRTSDAGPTKRKCGPERLISSNTVKHACLSRDAQVTSSSNGFPHEDSSAIRNPTTEDYRNTRDVILSRVLIGGKGHEQLQLSHSIHPHNFIQPAKPANLPPHYSALGSGQVVEALIKGYYDTFCSRIDSEIFKAAHTAIQGRHKSLPFALANRLKHYEPGKVLRVLSAKSQYKSVQISLDMTSLYKVLVISLYKKHLEILRELHIPLFEVRIMQIDLLDWVEQQIFAPRDSLPIIGTIRDNHPNREEDGFPGPIRDTQKELIEYFAQDHRDPRPDGIVTNLLKTFAIQNRDKYVYLKELSRLYWEDPQEILRGLEFDRKVALITTLADDFRIGTFRRQHISSAHKDYALFTTNFLQFEAGYPRTNFKRCEKRRYHPDLPLAMYYYVEVSNGYRILRILKFLPAASKSEQILQVDADALYQMFRRLARTVDHIHLKILNELEITAQETIQTKREVVLGALIEEILQPKFGFPIVGKIKVNADVPPWEDATYASSVVFGKVQLKFIKYFSEPPETEDVKGAAALFLASWYRDNHPTEFASFIEALEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQ59</accession>
    <name>H6QQ59_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_21021</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EHS64671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890377.1">
        <property type="nucleotide sequence ID" value="XM_003890328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQ59"/>
    <dbReference type="GeneID" id="13542750"/>
    <dbReference type="KEGG" id="pgr:PGTG_21021"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21021"/>
    <dbReference type="HOGENOM" id="CLU_1993748_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQ59"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003605722" description="Secreted protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS64671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="125" mass="13996" checksum="2D98F48A31E87532" modified="2012-04-18" version="1">MPVKHARAPTLLAAVAQPVLSWYVNVLRQEGRHPEDKQTPIAQLTCVPFTSSDRLPIVPSGWLPRNFSYCQASGQMRHYIQARDNCHEHAFGAANSMFTKLDTLSGGGGHNLRRTEKVKENSRQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQB2</accession>
    <name>H6QQB2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21072</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EHS64814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890335.1">
        <property type="nucleotide sequence ID" value="XM_003890286.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQB2"/>
    <dbReference type="GeneID" id="13542139"/>
    <dbReference type="KEGG" id="pgr:PGTG_21072"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21072"/>
    <dbReference type="HOGENOM" id="CLU_1876470_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQB2"/>
    <dbReference type="OrthoDB" id="2509022at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="136" mass="15079" checksum="1011D2D856BD0140" modified="2012-04-18" version="1">MDMDLGNVASTHDAGPSHISNDDTECNPYTVKSVLESVDVDLNPWSTQDDGVVDNQHAAPLSSYQHLAYRGETPNIGSNHLMPQDGFLENRLSDMPYYSHDALDLHEQWKTPHGSQYGAAFPATSTTQKINRHRVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQN8</accession>
    <name>H6QQN8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21217</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EHS62754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890171.1">
        <property type="nucleotide sequence ID" value="XM_003890122.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQN8"/>
    <dbReference type="GeneID" id="13541366"/>
    <dbReference type="KEGG" id="pgr:PGTG_21217"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21217"/>
    <dbReference type="HOGENOM" id="CLU_115526_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQN8"/>
    <dbReference type="OrthoDB" id="10472517at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="175"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="187" mass="20334" checksum="C4C4A41A34E537C6" modified="2012-04-18" version="1">MAGKVLRGNINLAHICEDKDCGSDVEDGPNDSLPVTLSPNWRSGSLTSILHCFDKMVQAQATHHQTIKNNLLVYGRSQRLFKTFTGIRGVPRGLPRDCYEDAWWAKLSPYEQQTISQASTIGLGDIAKNLEKHCLGTTITTGTNAPTGSSTQNNNKRRLDETSLTGGQGEASQEGPRTTTGETMNVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRZ8</accession>
    <name>H6QRZ8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21566</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EHS63433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889718.1">
        <property type="nucleotide sequence ID" value="XM_003889669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRZ8"/>
    <dbReference type="GeneID" id="13543106"/>
    <dbReference type="KEGG" id="pgr:PGTG_21566"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21566"/>
    <dbReference type="HOGENOM" id="CLU_2777165_0_0_1"/>
    <dbReference type="InParanoid" id="H6QRZ8"/>
    <dbReference type="OrthoDB" id="10303991at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="69" mass="7052" checksum="0717A43BD90CECF7" modified="2012-04-18" version="1">MTSIAKNHSPSITFAGGSANFPIVIADDPAKSPTQAISPVTAQKSANKTVEKKSPEKGPKASTSGNQEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSL6</accession>
    <name>H6QSL6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178295">
        <property type="protein sequence ID" value="EHS63738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889497.1">
        <property type="nucleotide sequence ID" value="XM_003889448.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSL6"/>
    <dbReference type="GeneID" id="13541867"/>
    <dbReference type="KEGG" id="pgr:PGTG_21830"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21830"/>
    <dbReference type="HOGENOM" id="CLU_2607148_0_0_1"/>
    <dbReference type="InParanoid" id="H6QSL6"/>
    <dbReference type="OrthoDB" id="2517283at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="79" mass="8678" checksum="E43F183FEF18C8F7" modified="2012-04-18" version="1">MAPAVPLDPLLQDTGSEQAHIEPSQQTSEVTESQRGDELGTKRSSSYTEAEDVQLCRSWIVISEDPLIAPHSPQNEFQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTU8</accession>
    <name>H6QTU8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22178</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178322">
        <property type="protein sequence ID" value="EHS64354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889064.1">
        <property type="nucleotide sequence ID" value="XM_003889015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTU8"/>
    <dbReference type="STRING" id="418459.H6QTU8"/>
    <dbReference type="GeneID" id="13541787"/>
    <dbReference type="KEGG" id="pgr:PGTG_22178"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22178"/>
    <dbReference type="eggNOG" id="ENOG502S1WT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003292_8_2_1"/>
    <dbReference type="InParanoid" id="H6QTU8"/>
    <dbReference type="OrthoDB" id="2503698at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052055">
        <property type="entry name" value="Hepadnavirus_pol/RT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050:SF7">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="532" mass="61529" checksum="B7D26D5535A44532" modified="2012-04-18" version="1">MNIEVWRDLLSKCNLLPEYEDVLSGFDRGFDQGIPAHRIEGLDWYTPENHRSSELAKEKIQESMKKELKAGRMLGPFSHAQMKSAFGFFRSNPLGAVVNGDGEIRPINDLSYPRNDEFVKSVNSYVKKEEFETTWDDFKIVSKFLSDDKRRFELGLFDWEKAYRQIPTRREQWRYLLVKDFDDNLLVDTRITFGGVAGCGSFGRPADAWKLIMKNQFKLVNVFRWVDDNLFVREENATVSMKEVVDKSDEMGVKTNVKKYSEFSCEQKFIGFVWNGLDKTVRLPEGKIEQRLTQIHPFMVPGASFDYEQAEVLVGRLNHVSYILPHLRCNLCSIYRWLKSWFFRKAKRPTPEDVLVDLAVWVETLTNFEHTRLIRWGPPLDVGWVGDASTSFGIGILVGKHWAQFRLLDPQGDPRRISFLETVAIRLGLIMLLKLRNQKGKSLIVWTDNTTTENGINNKKTKDKDANNEWMKIQRILVEESVDLVARRVKSKDNKADALSRGLRSGQLVKHQVVIKLPEDLGGLLEQVVFKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTV3</accession>
    <name>H6QTV3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_22187</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178322">
        <property type="protein sequence ID" value="EHS64363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889073.1">
        <property type="nucleotide sequence ID" value="XM_003889024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTV3"/>
    <dbReference type="STRING" id="418459.H6QTV3"/>
    <dbReference type="GeneID" id="13541796"/>
    <dbReference type="KEGG" id="pgr:PGTG_22187"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22187"/>
    <dbReference type="eggNOG" id="ENOG502S2AH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011407_0_1_1"/>
    <dbReference type="InParanoid" id="H6QTV3"/>
    <dbReference type="OrthoDB" id="3259803at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041320">
        <property type="entry name" value="CxC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040521">
        <property type="entry name" value="KDZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18802">
        <property type="entry name" value="CxC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18758">
        <property type="entry name" value="KDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxC1-like cysteine cluster associated with KDZ transposases" evidence="2">
        <location>
            <begin position="140"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="304"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS64363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="727" mass="83590" checksum="9A7C7DFB6812CF3A" modified="2012-04-18" version="1">MPRPSSDNLFTEQRSYRPRPTPLADQLRDLETLQRMQTGTDPRLLINPDIVLAHQQLEEDIHRQMNPNPADEENGPGENFNHYDPEPEAHEDNDSLIDQLNAHHTSVDQRNRHQTIKNHWTELIPTLHGAYLWLQVKTRNWTSSNAFENFSTDFCTCDRFTHRQVDLIDLNTQKRESIPFCQCTPDILHLLARGYIGSTPVAPQTAFSINLLGFHNHLWQWCTVGTLPFMKAMQAWLEERSPPLLTQSGKRRDLRTNFAAAIDVYRLLGDRTNNVIKDTLHLTKQQSLALDSCPACFGSPSSESFTQNSATLPGSTPQNHPMPPNTDSQNQNEPTTSNQATQNQQPPLVICLDGNFQHRHHFAGSRNYTELITPNKFIKPCSINEMNDYIKNQEEMHRVRGTRDRCADSHKAADDKRNKSTWKACDDTGLMGCCCRHDSAIYLANISDGGENRKYPLSILNQVLSDVDSTRDVRVLYDIGCNLKKFISLRHLFPEDTNRLTFGTSVFHSYVHNWKCQLEFSPRYNDGWGLSDGEGLERLWSFLSPLVSPLRYATRNHRLAAIAHKIQFHNERGKENLIHWLGHKYNKAISRKQESTRKLDSLYKLINPFASPRASYDIQFFEDQWNNQRQFQLNHTDADRERQERLASFLDREASLNRLRTQLNTMVSTDAGSYDNIFQSLLDIASSMEQQQEFARELPGEHNILIGQDGMPQTVFAVFLQPQLTPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>H6QU00</accession>
    <name>H6QU00_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_22250</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178326">
        <property type="protein sequence ID" value="EHS64414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889013.1">
        <property type="nucleotide sequence ID" value="XM_003888964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QU00"/>
    <dbReference type="GeneID" id="13540985"/>
    <dbReference type="KEGG" id="pgr:PGTG_22250"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22250"/>
    <dbReference type="eggNOG" id="ENOG502S1WT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003292_0_1_1"/>
    <dbReference type="InParanoid" id="H6QU00"/>
    <dbReference type="OrthoDB" id="2678913at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052055">
        <property type="entry name" value="Hepadnavirus_pol/RT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050:SF7">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47823">
        <property type="entry name" value="lambda integrase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS64414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="808" mass="92900" checksum="E7B415B70C037D9B" modified="2012-04-18" version="1">MNVDKWREALSVNNLLPQYSDVIDGFTHGFDQGIPQHAIDGKPWFTPENHKSSLLVRDKIEESIAKEIAAKRMMGPFSHQLMKATFGFFRSNPLGAVVNGDGQIRPINDLSFPRNDPEIKSVNSYVNKLDFETTWDDFKTVSKFLAEDKRQFELALFDWEKAYQQIPTRQDQWKYLLVQDFNGNLLINTRITFGGVAGCGSFGRPADAWKLIMKNHFDLVNIFRWVDDNLFIKEVEGFLSMKDVVVKSTELGVMTNIKKFSEFAEEQKFIGFVWNGKNKTVRLPEGKIEQRLNQIHPFQVEKAMFDYEEAEILVGRLNHVSYILPHLRCNLCSLYQWLKSWIWRKAKRPTPTNVLEDLRVWVDTLNNFEHMRLINWGPPLDKGKSLIVWTDNTTTENSINNMKTKDKEANDEWKRIQEILLRESVNLIARRVKSKDNKADALSRGLRSVDYCAPNKMQPSANESFFLSIRDFTSQGHLMKFPSKLDQHVLNGWSRSTLKSYNSGVRKFLLFKKTVGQERFLLPATEDDIYNFCFWAGKKDIDNGQSGVLSVTLKKYLQGLKAWHIFHNRPYPTISEKKVSLLLKSSQKIETLLAKKEPKKPVMIKHLISLVEDLATYEGEDAAILDLALVAFWSLARLGELTIDPSNPRRKIIREYVTIHPNGSSATIELKESKTAPPGESQFLQLKGMANSLCPVRALIRQMSACNRHDHLFGFRKDNKHVALTKYLVKRRLAILWDARGFTGLSGHSFRVGGASFQNALGIPPAHICKIGRWASASYKLYLRTYSEEDLSFSLKLLSRLDLQWRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>H6QU23</accession>
    <name>H6QU23_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22299</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178327">
        <property type="protein sequence ID" value="EHS64428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888991.1">
        <property type="nucleotide sequence ID" value="XM_003888942.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QU23"/>
    <dbReference type="GeneID" id="13541017"/>
    <dbReference type="KEGG" id="pgr:PGTG_22299"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22299"/>
    <dbReference type="HOGENOM" id="CLU_1548372_0_0_1"/>
    <dbReference type="InParanoid" id="H6QU23"/>
    <dbReference type="OrthoDB" id="2510781at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="173" mass="19305" checksum="2B493730C8BE9919" modified="2012-04-18" version="1">MVAPHTPPVTSPLAHPGQRPSKPVRFGSINCRPKSSLLIRLSATFEEPFLTSSITHSYRFTDEGKMEMFSEEGKSDVQGRRVNYRDMMPAPVSISFTLFPHLVILIHSRSPNLITNLSYSSSKHEAHLPTTSPDEIRNIPSESSKLARLITSDALMIQPIPFPVEYFDTSGQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>H6QU88</accession>
    <name>H6QU88_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178332">
        <property type="protein sequence ID" value="EHS64551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888928.1">
        <property type="nucleotide sequence ID" value="XM_003888879.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QU88"/>
    <dbReference type="GeneID" id="13541159"/>
    <dbReference type="KEGG" id="pgr:PGTG_22290"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22290"/>
    <dbReference type="HOGENOM" id="CLU_2741242_0_0_1"/>
    <dbReference type="InParanoid" id="H6QU88"/>
    <dbReference type="OrthoDB" id="10270442at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="71" mass="8076" checksum="7359680399BFBC36" modified="2012-04-18" version="1">MDSNQASSIGSCSRFQKEDPSRTPYLLNCYVPTRLLVYYHSMVVALEATADKKSVIQNDRIAFSSFWDIPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUH8</accession>
    <name>H6QUH8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22413</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178341">
        <property type="protein sequence ID" value="EHS64690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888839.1">
        <property type="nucleotide sequence ID" value="XM_003888790.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUH8"/>
    <dbReference type="GeneID" id="13542455"/>
    <dbReference type="KEGG" id="pgr:PGTG_22413"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22413"/>
    <dbReference type="HOGENOM" id="CLU_2607155_0_0_1"/>
    <dbReference type="InParanoid" id="H6QUH8"/>
    <dbReference type="OrthoDB" id="2510480at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="79" mass="8079" checksum="3EC86CAAE6D9B3E3" modified="2012-04-18" version="1">MTSESLDSARPKSVSTGPQPGLGSKIRSTLALPVGGANADGRLLGERLMGSSLRPSTSGEIANYAGQCKPVPVPLPSRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUL5</accession>
    <name>H6QUL5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22452</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178346">
        <property type="protein sequence ID" value="EHS64727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888791.1">
        <property type="nucleotide sequence ID" value="XM_003888742.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUL5"/>
    <dbReference type="STRING" id="418459.H6QUL5"/>
    <dbReference type="GeneID" id="13541597"/>
    <dbReference type="KEGG" id="pgr:PGTG_22452"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22452"/>
    <dbReference type="HOGENOM" id="CLU_2832408_0_0_1"/>
    <dbReference type="InParanoid" id="H6QUL5"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="66" mass="7320" checksum="86ABF5067C1AA067" modified="2012-04-18" version="1">MRGKNSSLKRFLRRKKRQNVITPQTEALKAKLSQRKQLHIQQQKQKAAQKDGSGGGTGGGALSRFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>H6QV04</accession>
    <name>H6QV04_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Enhancer of mRNA-decapping protein 4 WD40 repeat region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_22592</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat EDC4 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178366">
        <property type="protein sequence ID" value="EHS62617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888665.1">
        <property type="nucleotide sequence ID" value="XM_003888616.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QV04"/>
    <dbReference type="STRING" id="418459.H6QV04"/>
    <dbReference type="GeneID" id="13542265"/>
    <dbReference type="KEGG" id="pgr:PGTG_22592"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22592"/>
    <dbReference type="HOGENOM" id="CLU_259445_0_0_1"/>
    <dbReference type="InParanoid" id="H6QV04"/>
    <dbReference type="OrthoDB" id="21128at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045152">
        <property type="entry name" value="EDC4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15598">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15598:SF5">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="69"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="126"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="199"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="778"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="944"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="69"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="126"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="165"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="199"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="239"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="778"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="796"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="832"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="946"/>
            <end position="965"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS62617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1096" mass="121754" checksum="5D3ACA8F28ADE846" modified="2012-04-18" version="1">MAEQQQQQQQQLSLLELLHLNFTPKPKLETEEQQQQQMETSRYKYVSVLDNIVSVTLVDQLLLNTPSLTNTSLTPSQHNHTLNTPTTTTSTTDYNQLQPTTSTPKPTRSLKPITTATMQAINQLPSLDNQPLSPKTNTQPANTSTSTSSKAIPTEQQQPPSNQEKTIKPTTEENSEEIRIHKYMAIHLIDQIKNTPTTFFNPFSAESPQSQTQATSSEPIQNNDNHHPSILASPHPHRSTTENKEKQPKSNVGLRINSSFPPQELAISTGQANPEILYTAEPAPHVLPITLFPSSMVFSPGNRVAVDGFISYATKAGRIRVIDQNSGARMLLRKHEGPVLDMCIGRPRAVVGDLNSNTTSSRRWRCIASAGSDGRLVIWKVPVRFDEDTANYEILADILSVNSDLLLGPEKELLQAPSHLRFSTIRWHPHDPSIFLLSTNDHRLILVRLDRGAFAASWKRGTPLGKSLSELEAFEGQDVLRTNSDVVAFAFSPDGSAFAFVTEDKMLTVRQTSKPHWTIMGGQLPSSHGSITRLEFLATSAGVIKGFLITKRFGTRVEAVQLSEISEVVIGITLHPPEPVDDLPLLPCFGHSAWQAQYSTVLLSNSLRGSIFAFHLNFCENSSADDELLLLKPGHSEGPRLGAGTQPSDDDASYIERVAATRQSSQMQRSRSNSWIASRTMFVDQISEVPSPDPIISFVLDQGMRPPPGPSSSPVSVFNLHPKGIHQLYLPKDLIDFQTQSDQQPNPSQLAVPPTRSRSLAGEILVDVHVEHQVQKAEVLDFSDDDPKSKPLPPSVTANPVDSSNLDSKKPIPPSTSSKQDLKPRRSTHHQSTSSASLHPSLSVPPPSSSSNSAPVDQKAPPSFDGSLFYKELEKNRTSVIEQVHHLLGKSLDKQEQKLRNQFLAQQEIETRRHEHLLNILSTSLSSKNLGQIVKEQVKATLAVNDNHRDRDRNPNQNREPSDSDLKIIDGVRLAVQEGLKKNPHHHPQHHPELVVDRFHPSLGHEGLNKLRWIWSCINAIDEHDQQTAQYFDRVLDLCYLHLASRKSKLGLSPQLLNLHPANPLPQLHGNQEIDGINQVLWIIQDKLQKFKNLVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>H6QV08</accession>
    <name>H6QV08_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22617</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178367">
        <property type="protein sequence ID" value="EHS62621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888658.1">
        <property type="nucleotide sequence ID" value="XM_003888609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QV08"/>
    <dbReference type="STRING" id="418459.H6QV08"/>
    <dbReference type="GeneID" id="13542270"/>
    <dbReference type="KEGG" id="pgr:PGTG_22617"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22617"/>
    <dbReference type="eggNOG" id="KOG2304">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053015_1_0_1"/>
    <dbReference type="InParanoid" id="H6QV08"/>
    <dbReference type="OrthoDB" id="127208at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324:SF2">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="359" mass="40794" checksum="48412190645EDB3A" modified="2012-04-18" version="1">MSPKNSNLPFTIPGATVLTNNDQVNPTKHNKALVIAWDQDGEGGKSSMRIILDWLAIGENYQRWLWDSKLGSIKSPSINEILDTMIGSGITHRDHKGIQTKIKELHSFYAKVRDLLSHTRSGILDEEIKNGTIKLPATLAEMSQYWDELRPMMSIRILASRPVTSSSTDRHIPDLIGRRKTNIKVIPARQTKAAANPDDSQVNFGRRESPPSGGRDAPAAAASLVRTAGTKRKSPTQNPELAGFERIIAQNYEHRERVAEARERREQMKLANERRRDKMTRALERRKLKIEKRKVELLEMEVRAKARLLAVEDVYTRFRFVRDLKLLGYSSEQTENFLDKHFAGRGLLKNGALDQLRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>H6QVB3</accession>
    <name>H6QVB3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">NuA3 HAT complex component nto1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_22694</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
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        <property type="protein sequence ID" value="EHS62842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003888500.1"/>
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    <dbReference type="STRING" id="418459.H6QVB3"/>
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    <dbReference type="KEGG" id="pgr:PGTG_22694"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22694"/>
    <dbReference type="eggNOG" id="KOG0955">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001838_0_0_1"/>
    <dbReference type="InParanoid" id="H6QVB3"/>
    <dbReference type="OrthoDB" id="2499616at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15492">
        <property type="entry name" value="PHD_BRPF_JADE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05839">
        <property type="entry name" value="PWWP_BRPF"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Bromodomain containing 1, isoform CRA_a"/>
        <property type="match status" value="1"/>
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    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.920.10">
        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
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    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019542">
        <property type="entry name" value="Enhancer_polycomb-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034732">
        <property type="entry name" value="EPHD"/>
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    <dbReference type="InterPro" id="IPR050701">
        <property type="entry name" value="Histone_Mod_Regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000313">
        <property type="entry name" value="PWWP_dom"/>
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    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
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    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
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        <property type="entry name" value="Znf_PHD"/>
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        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793:SF107">
        <property type="entry name" value="BROMODOMAIN-CONTAINING PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13793">
        <property type="entry name" value="PHD FINGER PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="EPL1"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="PHD_2"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="zf-HC5HC2H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
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        <property type="entry name" value="BROMO"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Tudor/PWWP/MBT"/>
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        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PWWP"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PHD-type" evidence="11">
        <location>
            <begin position="140"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="13">
        <location>
            <begin position="194"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="Bromo" evidence="10">
        <location>
            <begin position="541"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="domain" description="PWWP" evidence="12">
        <location>
            <begin position="1731"/>
            <end position="1800"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="340"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="703"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
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            <begin position="785"/>
            <end position="848"/>
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            <begin position="902"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1166"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
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            <begin position="1341"/>
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            <begin position="1410"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
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            <begin position="1451"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1512"/>
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        </location>
    </feature>
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        <location>
            <begin position="1844"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="704"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="722"/>
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        </location>
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            <begin position="800"/>
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        </location>
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            <begin position="1025"/>
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            <end position="1202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
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            <begin position="1414"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1453"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
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            <begin position="1467"/>
            <end position="1477"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="9">
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            <end position="1591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
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            <begin position="1598"/>
            <end position="1619"/>
        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="9">
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            <begin position="1620"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1857"/>
            <end position="1873"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EHS62842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1912" mass="211170" checksum="DB6EE7B9AA060963" modified="2012-04-18" version="1">MGGHSMLPLELPKIDVPKVSYRIVGSKTLAEADITFSNSDQCSKHLKNYGYNSANTCELPPSYIRWVQPAEDELERGCEYDMDEQDLEWLQALNMERKRLSQEPVTCELFEIIMDRLEKEWFQLNQRVQKPDANMITVEDSRCAICEDGDTENSNAIVFCDGCNLAVHQDCYGVPYIPEGQWLCRKCTVSPDRPVTCELCPNSFGAFKQTSENKWAHLVCAIHIPETGVGNAMYMEPVDGVRCIPKQRWKLKCYICKKTVGACIQCANRSCCVAYHATCAQEVGLYVKMKPAGSLYPTTGARGTDEGPDSCAADHVGADGIRLRHIEALQAAAVARLSNNPATTTDSSDAKKTFDPQGSDSHLAISSKKINSLDDLRALKLLATGSQTSKSARAYHKSYSSGPPLVPEVIFQRVMQYSSKLRCAHRKTILNMICKYWSLKREVRRGAPLLKRLHLEPWTAAGGISSKNEEDRRRKYGLLVAVRHDLQQVKNLAAMICKREKIKLRKAEIQKEVIEKTLFPVYQRISLALTALIEADKQKYFLHPVSATEVPDYYDIIKHPMNWSTIQRKIDRFEYFRLSEFISDVHLTLTNARIYNHASSIYHKTAIRIGKAIEPLLQELLASEPFSPIAGLASESFPTRRQKEHFAEIPLVLLPDHISDLLDVHDLPGSTTRPYDEVLRDRRAKRSTQNQTNSLLNLLPAAMHSQNQRSSSTEPPSARSVPPSQSLFEGLNSNPDVPSITNNPCDIPTSNNLQNRRVEERLRKIAGSSVGNVRQPSSSIIASLPKASSVGISDPATPSADKELKIESSETRSTQKKKRRRTATDSSDPTTNRPLKRAAPRIKPQSQLDEEALIEKQNPNATRGQLISLKLRALHAKRIEDRERNMPDDEKARRRRLREYIREKRQQHRLSSRSQTTPANQSSSDPSHASTTPNETETSLSRDVSYTASNESQSNTNNLNEQAPQAPPELWKVTEDEKASSQAELPKESTNRASSNLDFEGTASASSPTGSGTAIADSNSRTSMQKHEDMSAGIDEKSEDIHSLVTPIDPIKLALCHQVDGVPQISHASEEATTLPTSETRTTSTILRTDAEEASVVSSAHPKITQQAAAVPVSLPQSYQSVNVSQGAAYLESSVMTNDVSERPYTPEDCEMICDGIVPTIASEHFAPSESQASTSDSANQPGGHSNHSQGSPAQNSDTDVQQAEVRSGTSLPHLPEHEIAIEEQMIQAGAMVPDEDMSLVISLPNGQAELVSLPSLNSHEDINDETTEGDRPLALDTTLSPNVAPVEDTHSTTVPVGTPTTLNVPEVAVPSASIQVRFDDPPIQIAPDVSTDVSLQPEFSLVNHMSSPRRPSTRSSKKLSLAQNEPTVGPVRQQPDLDPPIAQQTLVNSDLAANNAPVQSRQKLKIRIKKMDSSTSTRKSQSGETLLTEPIPISGSPKLRLILDRPPYNWKAGSSSTQDTTVGQEDVARNGKEATKAKTPKSAGSAGPPEFLTSLPGEKDSFRLFNTGFILPDRTRRHTPGPLANDSHPQSPERRESGSPEMTLRTTRVSARLKPVRLTLHHHPPIDQDTSAEASTSNCNPTGFSTNNQDRTFRAPASSAGDESGSSELSKSPSPSLDHTNSPEGNLNNSVEAQISLEAAQEECQVEMDDKDALEASCNGQKANKTSKAAKKVKPTRAQQEKELEMDSRGFLIYPKAPQNGAKRIHPLARDAFTRMSQATRLPEEGSIEDGTLVWAKVPGHPWFPAEVGLPEDPAVPQSMLDKKPPSEKMDHHVLVMFFDRQRSWQWVQRRNTRLLGESDELDALLSSEAFVSNKTILEEIQNGCAFARSNIEQTDDAKVQEAEVPDTNQQHHSTSSKDHDFRASRKEDKANDANYPDVTFDKALNRIEDMPVGLKRTRSCRFRPLNPPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>H6QVG4</accession>
    <name>H6QVG4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="5">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_22744</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="5"/>
    <dbReference type="EMBL" id="DS178416">
        <property type="protein sequence ID" value="EHS62947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888497.1">
        <property type="nucleotide sequence ID" value="XM_003888448.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QVG4"/>
    <dbReference type="GeneID" id="13540849"/>
    <dbReference type="KEGG" id="pgr:PGTG_22744"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22744"/>
    <dbReference type="HOGENOM" id="CLU_1661669_0_0_1"/>
    <dbReference type="InParanoid" id="H6QVG4"/>
    <dbReference type="OrthoDB" id="10382969at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS62947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="159" mass="17626" checksum="4364CD4A55E6CD4C" modified="2012-04-18" version="1">MQVSKVIHEARRIPGGHNDAHSTFARRFHACTGDMSKEETTNKFAAGEFPVIACTMALGLGQNWKRVRSVVHVGQGDPASICQMIGRCGRGGSPGLAILFVEANQRSGKNTVDEFANPGLDMYHYETMIQMLLWSALEKSMFIFPIAYVQTAILRRRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>H6QVN1</accession>
    <name>H6QVN1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hydrophobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22802</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS989980">
        <property type="protein sequence ID" value="EHS63418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890869.1">
        <property type="nucleotide sequence ID" value="XM_003890820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QVN1"/>
    <dbReference type="GeneID" id="13541077"/>
    <dbReference type="KEGG" id="pgr:PGTG_22802"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22802"/>
    <dbReference type="HOGENOM" id="CLU_2575006_0_0_1"/>
    <dbReference type="InParanoid" id="H6QVN1"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003605835" description="Hydrophobin" evidence="1">
        <location>
            <begin position="27"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="8352" checksum="8A07485AB9E4E13D" modified="2012-04-18" version="1">MNFNISKIPQAFGIILMMGMVSEILAGASCPSGSAFMCSGNSGLYSPPKSQTCNGSDSLMCCQVGLPPPAQNCVAPSYNVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>E3JPW7</accession>
    <name>E3JPW7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA-directed RNA polymerase RpoA/D/Rpb3-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_00006</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74050.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307056.2">
        <property type="nucleotide sequence ID" value="XM_003307008.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JPW7"/>
    <dbReference type="FunCoup" id="E3JPW7">
        <property type="interactions" value="548"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JPW7"/>
    <dbReference type="GeneID" id="10528083"/>
    <dbReference type="KEGG" id="pgr:PGTG_00006"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00006"/>
    <dbReference type="eggNOG" id="KOG1522">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038421_1_1_1"/>
    <dbReference type="InParanoid" id="E3JPW7"/>
    <dbReference type="OrthoDB" id="270173at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07031">
        <property type="entry name" value="RNAP_II_RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.120.12:FF:000010">
        <property type="entry name" value="Related to RPB3-DNA-directed RNA polymerase II chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="entry name" value="DNA-directed RNA polymerase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00320">
        <property type="entry name" value="RNApol_arch_Rpo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001514">
        <property type="entry name" value="DNA-dir_RNA_pol_30-40kDasu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022842">
        <property type="entry name" value="RNAP_Rpo3/Rpb3/RPAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050518">
        <property type="entry name" value="Rpo3/RPB3_RNA_Pol_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800:SF2">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="Insert subdomain of RNA polymerase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00446">
        <property type="entry name" value="RNA_POL_D_30KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RpoA/D/Rpb3-type" evidence="5">
        <location>
            <begin position="30"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="309"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="325"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="345"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP74050.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="365" mass="39473" checksum="46059E360B949D56" modified="2012-04-18" version="2">MSTAPLPLEPTQSTDGSQLQVKVTELDDQHAAFILEGIELGLANSLRRTMIADLTTIAIHSVEFEENTSVLPDEMIAHRLGLIPLISEDLDKYLKNWQRDCTCLSYCDDCSIELVLSTKCTLVGDVLAVNSHDLVITPREDGAHRGDVGKPYQFTMPGGEQPGVLITKLRKGQEVRLKCRAIKGIGQEHAKWSPVSTIGFEYDPYNRLGHTDLWFEVGTDPKAEWPLTKNAKYERPPLDDEPVDYTSKPSRFYFDVESAGSLKPDDIVIKGIESLIVKLASVQQGIQAALGEGGGLNPDGRNTPYMGTPFGGAGGATPGSRPDGTYSTYGGATPNARNFSDWGPPAAPLNPPGGPSTNGYSGGWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3JSI5</accession>
    <name>E3JSI5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6589 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_01551</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP74958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319377.1">
        <property type="nucleotide sequence ID" value="XM_003319329.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JSI5"/>
    <dbReference type="GeneID" id="10547240"/>
    <dbReference type="KEGG" id="pgr:PGTG_01551"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01551"/>
    <dbReference type="HOGENOM" id="CLU_009176_0_0_1"/>
    <dbReference type="InParanoid" id="E3JSI5"/>
    <dbReference type="OMA" id="QNIPMAP"/>
    <dbReference type="OrthoDB" id="2495569at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046496">
        <property type="entry name" value="DUF6589"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20231">
        <property type="entry name" value="DUF6589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6589" evidence="2">
        <location>
            <begin position="352"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP74958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="839" mass="94873" checksum="9F8F96B2FF155BA9" modified="2011-01-11" version="1">MESPAETLKVLNICKLLNSTPTKMTPKRLLHIFLESNNSEIAYLRRLWARPRGLDSTIQLLNLLRDEILSSEGGRARWSDFIQQEAISILVSQESPRGNHPAGSFHSSLTVKEAFFTAEEQRVQEIAMTSEDMPFLYGLMGGMLRGHGHDKASSADNEPSASPDLSEDHPGSTNDQATPEDFAEVSYYLALSGEERKNARIDRIATTICSIMAFARNRRANGLQLKNAVRFTACGVSERVQEYLHYLGLSASRRTAMSALKTLASEGEEAIKKVMAWEENTPIAPTICIDNIDMEQRVHDISVGRRSNTFRGTWGYIHVPNPSLLSSLNMEELTLQAYQESIQNFRTFSIEPKHFMPTPEAKISEVEVWKSQIARVLMEYIALPLDKSKAIPTEPAVIDQISAEKPTIHMLKLMDVSDNSAESIGQVFATIHKQSGLSDKHFYGRLQPMDGDLGTIQNFNSLRSQRAPSPYPKENLDNVIFQLGASHTLWNIASAIFTHHFGDPSDQTNCGAWHFLEAIGFPADKAIQKKDFTLMINQMEKVMEAMLYYCLRVIMKNQFQKLGEDRLSIPTSQWNQIVNDCYEQFCSPEARSTAARQVSPKLSNLLIMLHDFSSVVEAKRSMRAGDIGRLINVWKKWCLMTQGLKGLTNYSSYLPRMVLLLTEILPPALRKYLSHNLLFSPTGRANHFVAKDHWLECQNYWLKFVYNNTGNGTNVDRLRDLFSVNIILLQGMFQSLKVDCGATVIYQSHKNSLTQRSLDMFLQMAVNRHVLDQTMSKPKSPINRIDNTLLTGLAKFKRTIRSKDSQLKKMKKHLLYTGQAHAIQDEEEADSEPEHDSGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3JT77</accession>
    <name>E3JT77_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_01949</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75356.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319775.2">
        <property type="nucleotide sequence ID" value="XM_003319727.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JT77"/>
    <dbReference type="GeneID" id="10542903"/>
    <dbReference type="KEGG" id="pgr:PGTG_01949"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01949"/>
    <dbReference type="HOGENOM" id="CLU_2224501_0_0_1"/>
    <dbReference type="InParanoid" id="E3JT77"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP75356.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="106" mass="12230" checksum="514C789E21E7B5B3" modified="2012-04-18" version="2">MALPPVFERFRTARVTRLLKSSSIYNRSTLKTLFSLTDDQRSSLFSALEDFELDPVQLRLWGKRLPPRVRKGLLNLRHQPKLCLKHLCRNSLPISSLLPSTCDPNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWD1</accession>
    <name>E3JWD1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="30">Cystathionine beta-lyase</fullName>
            <ecNumber evidence="5">2.7.1.36</ecNumber>
            <ecNumber evidence="6">4.4.1.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="27">Cysteine-S-conjugate beta-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="31">Ergosterol biosynthesis protein 12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="35" type="ORF">PGTG_02797</name>
    </gene>
    <organism evidence="35 36">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="36" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="36">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="24">
            <text>(R)-mevalonate + ATP = (R)-5-phosphomevalonate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17065"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.36"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="24">
            <dbReference type="Rhea" id="RHEA:17066"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="28">
            <text>L,L-cystathionine + H2O = L-homocysteine + pyruvate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13965"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:58161"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="29">
            <text>an S-substituted L-cysteine + H2O = a thiol + pyruvate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:18121"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29256"/>
            <dbReference type="ChEBI" id="CHEBI:58717"/>
            <dbReference type="EC" id="4.4.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="26">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homocysteine from L-cystathionine: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="25">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the GHMP kinase family. Mevalonate kinase subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the trans-sulfuration enzymes family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.36" evidence="5"/>
    <dbReference type="EC" id="4.4.1.13" evidence="6"/>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320775.1">
        <property type="nucleotide sequence ID" value="XM_003320727.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JWD1"/>
    <dbReference type="STRING" id="418459.E3JWD1"/>
    <dbReference type="GeneID" id="10534249"/>
    <dbReference type="KEGG" id="pgr:PGTG_02797"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02797"/>
    <dbReference type="eggNOG" id="KOG0053">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1511">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009484_1_0_1"/>
    <dbReference type="InParanoid" id="E3JWD1"/>
    <dbReference type="OMA" id="QPYRFST"/>
    <dbReference type="OrthoDB" id="2545919at2759"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00098"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016846">
        <property type="term" value="F:carbon-sulfur lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047804">
        <property type="term" value="F:cysteine-S-conjugate beta-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004496">
        <property type="term" value="F:mevalonate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071266">
        <property type="term" value="P:'de novo' L-methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019346">
        <property type="term" value="P:transsulfuration"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00614">
        <property type="entry name" value="CGS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000013">
        <property type="entry name" value="Cystathionine beta-lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000009">
        <property type="entry name" value="Cystathionine gamma-synthase homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000027">
        <property type="entry name" value="Mevalonate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.890:FF:000003">
        <property type="entry name" value="Mevalonate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000277">
        <property type="entry name" value="Cys/Met-Metab_PyrdxlP-dep_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006238">
        <property type="entry name" value="Cys_b_lyase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054542">
        <property type="entry name" value="Cys_met_metab_PP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006205">
        <property type="entry name" value="Mev_gal_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01329">
        <property type="entry name" value="cysta_beta_ly_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00549">
        <property type="entry name" value="mevalon_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808:SF50">
        <property type="entry name" value="CYSTATHIONINE BETA-LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11808">
        <property type="entry name" value="TRANS-SULFURATION ENZYME FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01053">
        <property type="entry name" value="Cys_Met_Meta_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00959">
        <property type="entry name" value="MEVGALKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00868">
        <property type="entry name" value="CYS_MET_METAB_PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="8" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="19" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="23" id="KW-0456">Lyase</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="36" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="17" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="22" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="18" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="20" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="33">
        <location>
            <begin position="593"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="34">
        <location>
            <begin position="777"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="32">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="32">
        <location>
            <begin position="895"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="32">
        <location>
            <begin position="897"/>
            <end position="917"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="24">
        <source>
            <dbReference type="ARBA" id="ARBA00029310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="25">
        <source>
            <dbReference type="ARBA" id="ARBA00029438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="26">
        <source>
            <dbReference type="ARBA" id="ARBA00046315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="27">
        <source>
            <dbReference type="ARBA" id="ARBA00047213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="28">
        <source>
            <dbReference type="ARBA" id="ARBA00047517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="29">
        <source>
            <dbReference type="ARBA" id="ARBA00047625"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="30">
        <source>
            <dbReference type="ARBA" id="ARBA00072331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="31">
        <source>
            <dbReference type="ARBA" id="ARBA00084043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="32">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="33">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="34">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="35">
        <source>
            <dbReference type="EMBL" id="EFP76356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="36">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="932" mass="100877" checksum="307C63DE0FEDC72B" modified="2011-01-11" version="1">MNNNNNNSSTLDQSTNSSSDQSSPLSASVVSSSSRVARQPAPHRFSTLCATVENPDQDHQDRYGSSSVPIYQSSTFKGVDGLYDYTRSGNPSRTFLQHHVAKISKAKHAFAVSSGMAALDVILRTLQPGDEIIAGNDLYGGSNRLLTYVKEHNGIITHHIDTTNLENLKPFLVKDSRVRMVLLESPTNPLLQIVDIEAVINLVKAVIPEAIIVVDNTMMSPYLMKPLEFGADVVYDSGTKYLSGHHDVMAGLVTCNSDKIAKQLAFVINSIGNGLAPFECFLLTRGIKTLSLRMDRQQASAIRVAKYLDQLGFKVNFPGLAHHKGKEIHDRLAKGPGAVLSFETGDKKLSEKIVSSARLWSVSVSFGCVNSLISMPCLMSHASIDPAVRAARNLPEDLIRLCVGIEDPDDLLEELQAALVEAGAIRILDETRVDSGYERVGMNSLPISPAEAMIPQKDYLGSSGNPTTLLVSAPGKIILFGEHAVVYGKKAIAAAVNRRCYCFVEPGGYQTIETVALCLPDSGWSGSWPINQLPWDCVKPTGEGELHVNPQLSQALLTKVAGQADKNSVLQAAHAFLYLFMHLATKENRRSQVFTIRSTLPIGAGLGSSASYSVCLASALLYSHQHIAIPTQKGIETTQVETINRWAFLGEKILHGSPSGVDNTVSSFGGAICFQKLIGENDQQKRQSIEQIQGFGAIRILISDTQVPRDTKSLVAGVAQKKEKDPKLVQSILDEIEELTISAQESIKELAVIKQPDSKDGQNPDQELLKTKRNKILNQLGHLMARNHSLLSSLGVGHSQLDLVKSFAANCGLETKLTGAGGGGCAITLIPDNFEEEKLSTIVTNIEAHSMKAYETEIGVSGVGILNNLDHHHQLLESVKKIYLTSDLTTLSQSLSNPSQALSRVNPQPDTISNGDSSAPGVPTRDLFWSFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWN6</accession>
    <name>E3JWN6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02902</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76461.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02902"/>
    <dbReference type="eggNOG" id="ENOG502SBYH">
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    <dbReference type="InParanoid" id="E3JWN6"/>
    <dbReference type="OrthoDB" id="2507436at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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            <begin position="32"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76461.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="593" mass="67149" checksum="F60FEF9DC6152E92" modified="2012-04-18" version="2">MVFSSIRRHINYPKHKELEKNFLESRVTHVEDTINHPESRPSPHENANSPGLEVDDPALDVLNYQTTQSWTACEDLATNGKDNHEFLDAGESNTEDNTFSDNELDSCASDLDTSSDLSDLNSNDEADGEDFELSAEDLDLMRELGRAGDGDLASTTPRPHVDRSWWPFRTKEVCLQLIPVHDACLFYLFCQYMIASLVIGYAHAILSQSIYDRIRSMFSLVHVILPDWTTNRREKKKHRDLLQMDVNKSYSILGNPTYALSIQKILAQEVGNPLVTRAMDYYPEDARGKNIFKLSQSKKWLEDMDPDSRAPMVRTTNGDFYLFEPVQLSNNSVVIPIFFYKRGEDLWSKCVNPAFEPESNGHNLRITIPWRVNFHSDIAHEINISEFYCAFPNIAGPDGLMLSQRCQGRLYELGDHMTVIHLLPNPWRNHAKGRMIRHMPITLYCDDTSGNQSKRWNKHVSFYFTLSGLPPNWSNQQYNCHYLTTSNVANAMELAGPIVDEMNQLSTTGAVAWDAGLMEEVLVTSNVLCFLGDSPMHAEATMDAHRDYSGVPVEAVDCRKQIRGVHEGLAQWHLAGTKTGPPEPPVVVDPSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
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    <name>E3JX55_PUCGT</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_02091</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76630.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003321001.2"/>
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    <dbReference type="AlphaFoldDB" id="E3JX55"/>
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    <dbReference type="KEGG" id="pgr:PGTG_02091"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02091"/>
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    </dbReference>
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        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
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    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP76630.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="385" mass="44617" checksum="A96DC967392DDE4D" modified="2012-04-18" version="2">MVWVNGRVDRLVQALKTLKSKGRKKNRGLIRSIMFLKKNDCSIEWEVRSSVCWILRTKQLPLLHHHVHELLKCLDPASLHLDTNQRFQKGLEILSEIDNCMDQISVSIGSVWDLPRPVDSQENVEALTPFRRHRLVHNSEGILGLVSDVLGRFEDFISHFFVSDDSTLRTPSTNNSLVRLAVQDMKVANTLIESLGQSDFVVFLRQWSFTERKRNLDCPLTSLIILRNSSQIKGNPEKSLVRKQIEGLVALHKLHRVLVLKICRFPNIKPGLIPETSFLELEGLRVTTSTLEYELEQIMQYLGHTRRPDFVKLTLAMDDLEAAFKRVKGLLNQAFASPNYNFDRETYQYSEQWIQLWISQFNIAANNFFWISNAYITEKLSSIYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXZ4</accession>
    <name>E3JXZ4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7143 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_02380</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76919.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="E3JXZ4"/>
    <dbReference type="GeneID" id="10529104"/>
    <dbReference type="KEGG" id="pgr:PGTG_02380"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02380"/>
    <dbReference type="eggNOG" id="ENOG502S5W9">
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    <dbReference type="HOGENOM" id="CLU_1511325_0_0_1"/>
    <dbReference type="InParanoid" id="E3JXZ4"/>
    <dbReference type="OrthoDB" id="2501656at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055567">
        <property type="entry name" value="DUF7143"/>
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    <dbReference type="PANTHER" id="PTHR37592">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37592:SF1">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23631">
        <property type="entry name" value="DUF7143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7143" evidence="2">
        <location>
            <begin position="1"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="161"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP76919.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="178" mass="20031" checksum="B9763A6601A7D2B8" modified="2012-04-18" version="2">MTGRHNVPHGVIINTHVQCLEGSGPFHDIPDVVYDNMQYSVYDYQKYPNLSPVGFALEYFTPHKRTKSITKALENLMVLYGATNAGLRSWGEARSNDVKKIKGPSFYLAFQHAISIENLELAADQLKKVLKNCVDCKHGERERVIKIARQNNVKVPESLESDSQSQSLHSQSSLIDSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3JYC2</accession>
    <name>E3JYC2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03003</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321466.1">
        <property type="nucleotide sequence ID" value="XM_003321418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JYC2"/>
    <dbReference type="GeneID" id="10529394"/>
    <dbReference type="KEGG" id="pgr:PGTG_03003"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03003"/>
    <dbReference type="HOGENOM" id="CLU_943770_0_0_1"/>
    <dbReference type="InParanoid" id="E3JYC2"/>
    <dbReference type="OrthoDB" id="10438444at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="295" mass="32456" checksum="88B0091F4E052A37" modified="2011-01-11" version="1">MEESSSEIQSPLTENFPPVPSATLDSCPLILENDPSLEHLEKEETKNLGLETEILPIPSEISLPCALIEENYSPAVSVTLEFPPLISQNCEELEPLDNKEQQNPGPEISPFQVEEIPDKPVPGFAKFREILKQKYTVEDNSNPEDASNLEETEGNKLGLFLDSKNQLQYPRNDIMVAKPEGIGTQVDPSSGIRPIIFSQIFSPSEDIRDSLEPELPEILEESFGEKVTVESFTNTLIPVNVPALNPIVSDQLPTNISEVLTTEKTEAPDSTSSEIPQFGSPKICTQKDSPSLGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3K121</accession>
    <name>E3K121_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal protein L15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_03952</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL15 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP77996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322415.1">
        <property type="nucleotide sequence ID" value="XM_003322367.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K121"/>
    <dbReference type="FunCoup" id="E3K121">
        <property type="interactions" value="526"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K121"/>
    <dbReference type="GeneID" id="10541304"/>
    <dbReference type="KEGG" id="pgr:PGTG_03952"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03952"/>
    <dbReference type="eggNOG" id="KOG1678">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_080796_0_0_1"/>
    <dbReference type="InParanoid" id="E3K121"/>
    <dbReference type="OMA" id="YIRDAWK"/>
    <dbReference type="OrthoDB" id="10255148at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1120.10:FF:000001">
        <property type="entry name" value="Ribosomal protein L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1120.10">
        <property type="entry name" value="Ribosomal protein l15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024794">
        <property type="entry name" value="Rbsml_eL15_core_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000439">
        <property type="entry name" value="Ribosomal_eL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020925">
        <property type="entry name" value="Ribosomal_eL15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003269">
        <property type="entry name" value="PRK04243.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847:SF4">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11847">
        <property type="entry name" value="RIBOSOMAL PROTEIN L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00827">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01384">
        <property type="entry name" value="Ribosomal_L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01194">
        <property type="entry name" value="RIBOSOMAL_L15E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="160"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="169"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP77996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="201" mass="24008" checksum="2D388E7426CC1026" modified="2011-01-11" version="1">MGAYKYIEELQKKKQSDVLRFLLRVRCWEYRQLNVIHRASRPSRPDKARRLGYKAKQGFVIYRIRVRRGNRKKPVKKGATFGKPVRQGVNHLKFQRSLRSTAEERVARRCGNLRVLNSYWVNQDGVYKYYEVILVDPSHKAIRRDPRMNWICDPVHKRRESRGLTGAGKKNRGIGKGHRHNHQPKNAVWRRHNTLSLRRYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3K1H2</accession>
    <name>E3K1H2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF659 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04103</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP78147.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003322518.2"/>
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    <dbReference type="AlphaFoldDB" id="E3K1H2"/>
    <dbReference type="GeneID" id="10541777"/>
    <dbReference type="KEGG" id="pgr:PGTG_04103"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04103"/>
    <dbReference type="eggNOG" id="ENOG502SPG0">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_013565_0_0_1"/>
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    <dbReference type="OrthoDB" id="2748837at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052717">
        <property type="entry name" value="Vacuolar_transposase_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46169">
        <property type="entry name" value="DNA REPLICATION-RELATED ELEMENT FACTOR, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46169:SF15">
        <property type="entry name" value="INNER CENTROMERE PROTEIN A-LIKE ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78147.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="504" mass="55904" checksum="9CFE121C16E0D60F" modified="2012-04-18" version="2">MAPGRAAPAPAKDAPLQRAWLAPVGAHFFKSGADNPSVDKWQSPNGFDILGIVIYRLVEEASPAKLEAMPLDFIRLSKSHTGEYLANTVRLVAEKFGIQDKICGLVTDNAKNNELMVRELKKLKWPCFKGEAQWIRCFAHILNLIVQAILRPFGTPKKKGATKNPGSNSDGIDSDEDLAEEQISMLPRGHKPTLQDEESLHNLSDVDSNSESDEDKTESLSEADIKQASEEDKEDCYTSESCKQTLAKFRVIAKKLRFSPNSKAEFVEICQEKVCATPHNIERDVRTQWNSTSAQLNSIIRCEDAIVNRAYQLDEGDFKLARDLVEVLNVFYKITLQLSVAGSARLSNIVLYIDQITEHLSTVISNENYPAALRNTCRLGLKVTNKDEYFKLANWEPEWISKAIRLARDMWILFYKPKPITLSSPAPAPNTTSSSKVKTLDISLLSTKTEAVFIHFSQPKTGMLAGLGHTAAARGGDFSSDPFDLWLAGGLVLNGTVWNCSASE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K1J8</accession>
    <name>E3K1J8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04129</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP78173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003322544.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K1J8"/>
    <dbReference type="GeneID" id="10541803"/>
    <dbReference type="KEGG" id="pgr:PGTG_04129"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04129"/>
    <dbReference type="HOGENOM" id="CLU_1138484_0_0_1"/>
    <dbReference type="InParanoid" id="E3K1J8"/>
    <dbReference type="OMA" id="PREDCFV"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="112"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="244" mass="25693" checksum="98B3080A64CAB528" modified="2011-01-11" version="1">MIPVQIIGNRSYWTWIPHIWPHEVIVPFVRNGALPPPPEECFVLGAPLPGSDAGEEATSTRSSVEPPPSPLATSPTPDPTGTRSTVEAPPSPSSWVINTPSSTTPTPPLVESPSYHSSSARSSSVHEWPASSDFDSDGPAVDPPLSSPRQDNGVNISEVKAPSSGQRSLVAAEGVISGPSSSNIRGSRVARDDPPSSSGIEMLDGPPLSWKSKDYETIKRVRSSSPDVEVVSKKSGSLKACRST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3K1P7</accession>
    <name>E3K1P7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_04178</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP78222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003322593.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K1P7"/>
    <dbReference type="GeneID" id="10542014"/>
    <dbReference type="KEGG" id="pgr:PGTG_04178"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04178"/>
    <dbReference type="HOGENOM" id="CLU_128330_0_0_1"/>
    <dbReference type="InParanoid" id="E3K1P7"/>
    <dbReference type="OMA" id="LIFTMKE"/>
    <dbReference type="OrthoDB" id="2502482at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP78222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="149" mass="16454" checksum="C4676CB14C4AE362" modified="2011-01-11" version="1">MESPVIKAIYPLDGKIHETGSRTRFEATHQNSVGVQLGVDQYGKATLNRAQSIRYHGSTAPHVSASGVHTSELDLTMNEDSCLKHGVARSLYFAALIEFDSAADRSFEASLIVETQSQKELPFKLWSIPKTWVLDYDGKTELGQLNLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2W8</accession>
    <name>E3K2W8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_04244</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GMC oxidoreductase family.</text>
    </comment>
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        <property type="protein sequence ID" value="EFP78288.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="XP_003322707.2">
        <property type="nucleotide sequence ID" value="XM_003322659.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2W8"/>
    <dbReference type="STRING" id="418459.E3K2W8"/>
    <dbReference type="GeneID" id="10542074"/>
    <dbReference type="KEGG" id="pgr:PGTG_04244"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04244"/>
    <dbReference type="eggNOG" id="KOG0800">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1238">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002865_6_3_1"/>
    <dbReference type="InParanoid" id="E3K2W8"/>
    <dbReference type="OrthoDB" id="269227at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016614">
        <property type="term" value="F:oxidoreductase activity, acting on CH-OH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.560.10">
        <property type="entry name" value="Glucose Oxidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012132">
        <property type="entry name" value="GMC_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000172">
        <property type="entry name" value="GMC_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007867">
        <property type="entry name" value="GMC_OxRtase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552:SF138">
        <property type="entry name" value="DEHYDROGENASE PKFF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11552">
        <property type="entry name" value="GLUCOSE-METHANOL-CHOLINE GMC OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05199">
        <property type="entry name" value="GMC_oxred_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00732">
        <property type="entry name" value="GMC_oxred_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000137">
        <property type="entry name" value="Alcohol_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00624">
        <property type="entry name" value="GMC_OXRED_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0274">FAD</keyword>
    <keyword evidence="5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172974" description="Glucose-methanol-choline oxidoreductase N-terminal domain-containing protein" evidence="6">
        <location>
            <begin position="20"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="domain" description="Glucose-methanol-choline oxidoreductase N-terminal" evidence="7">
        <location>
            <begin position="329"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="568"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="611"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="567"/>
            <end position="568"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="612"/>
            <end position="613"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000137-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP78288.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="632" mass="69605" checksum="B7E430F29CCB8929" modified="2012-04-18" version="2">MVSICFVACLLICFMGVNPLTGISEDHLRFDNPTHQVRGSLMKRKTHHYIIVGAGTAGMTIAARLSEDPHVQVAVVEAGFDYDLSPVNKRLVDTPGTDTIGCGGKQYDNMADAVDWRFTTTPQSGANNRTVRYARGKTIGGSSARNFMIYHRASKGSLDKWAELTGDPDWTFEKRFDDYQKSVSFTPPKHDLRQELPAAQCDPLAFTRSNGPVQISYPNTAQPFSKYMQLSLNEKGIPTCQDFNGGSLSGVQYASTTIDPKDSHRSSSRAFLDAARTRNNLVVYTSAMVKRIIFDESTPPRAQGIEFLNTLAGSSDQLFAKKEVILSAGTFQSPQLLMVSGIGPRDQLTANKIPIRVENPNVGRGMQDHIFFGPTHPVHTIETLTRIAVNTDYSLSQYLNFTRRQLGPMTNNAADMISFERFNTSKLLQLKAQSLSDYPADWPHVEYLSAGGLVGDFSDLHRLNLKAGMATGKEFVTILAALVAPQSRGTVKITSNDASVPPLIDPGWLTDPVDQRIAVEAFKRTREFFSAKAMHPILDGQEYLPGPSVNSDDQILDWIKNNLMTVWHAACTCAMRTKDQGGVLDSHFKVYDTKGLRVVDASAFPALPPGHPQSTVYMIAERAACLIRKGKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2Y5</accession>
    <name>E3K2Y5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_04261</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322724.1">
        <property type="nucleotide sequence ID" value="XM_003322676.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2Y5"/>
    <dbReference type="GeneID" id="10542258"/>
    <dbReference type="KEGG" id="pgr:PGTG_04261"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04261"/>
    <dbReference type="eggNOG" id="KOG0265">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090077_0_0_1"/>
    <dbReference type="InParanoid" id="E3K2Y5"/>
    <dbReference type="OrthoDB" id="10277729at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP78305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="243" mass="27836" checksum="7DE18891DF42E530" modified="2011-01-11" version="1">MDQVKKTTANNTPQLTFDIQFVSAFGLTVFCNTSKKEPMGSTSFQETTPPTLSDLSSNQILKLDRHWNLRPWLAHNIEDSVKEILLTISNPIFDNLERMMVWELNVYDCFKEANAFDPIRTKSVLNKWFPQQGDWELFFPTMDLLKSTSRFFSDDDTPITFLGFTLEDAQALLQLDYQPPASYSSSGSGQWDIRKKELAKHLVTMMDEMRKAQGYSSMILDAHFESLDLADEGTEASEPHLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2Z9</accession>
    <name>E3K2Z9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_04275</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322738.1">
        <property type="nucleotide sequence ID" value="XM_003322690.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2Z9"/>
    <dbReference type="GeneID" id="10542273"/>
    <dbReference type="KEGG" id="pgr:PGTG_04275"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04275"/>
    <dbReference type="HOGENOM" id="CLU_2134736_0_0_1"/>
    <dbReference type="InParanoid" id="E3K2Z9"/>
    <dbReference type="OMA" id="DSYWDPT"/>
    <dbReference type="OrthoDB" id="2510077at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172354" evidence="2">
        <location>
            <begin position="19"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="86"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP78319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="113" mass="11992" checksum="E64106E68DD4FB39" modified="2011-01-11" version="1">MNSFAFMSMLLFSSTTLGMPNGYSYTNYPSQASEYYSVMYGDAPHYDSGYLDNQPSTDTYWDPTLAPSYPYGRSVSISGADMKTDPSPSSGSIPAGGDPSHTPAGARSSAGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3B7</accession>
    <name>E3K3B7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNA polymerase II transcription factor B subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_04930</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the TFB2 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78974.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323393.2">
        <property type="nucleotide sequence ID" value="XM_003323345.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3B7"/>
    <dbReference type="FunCoup" id="E3K3B7">
        <property type="interactions" value="239"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K3B7"/>
    <dbReference type="GeneID" id="10537162"/>
    <dbReference type="KEGG" id="pgr:PGTG_04930"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04930"/>
    <dbReference type="eggNOG" id="KOG3471">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015563_0_0_1"/>
    <dbReference type="InParanoid" id="E3K3B7"/>
    <dbReference type="OrthoDB" id="364513at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000439">
        <property type="term" value="C:transcription factor TFIIH core complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005675">
        <property type="term" value="C:transcription factor TFIIH holo complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001671">
        <property type="term" value="F:ATPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2610:FF:000001">
        <property type="entry name" value="General transcription factor IIH subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040662">
        <property type="entry name" value="Tfb2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004598">
        <property type="entry name" value="TFIIH_p52/Tfb2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00625">
        <property type="entry name" value="tfb2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13152:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13152">
        <property type="entry name" value="TFIIH, POLYPEPTIDE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03849">
        <property type="entry name" value="Tfb2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18307">
        <property type="entry name" value="Tfb2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 9" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription factor Tfb2 C-terminal" evidence="11">
        <location>
            <begin position="436"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="810"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="812"/>
            <end position="832"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP78974.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="887" mass="99551" checksum="D7ADDADA46139799" modified="2012-04-18" version="2">MSSLRTTTTIPANKTRTTTTADFSSALYAFLNALPKLTLARLYNTPASCLAIFRSLTLSCLIDSSLIFCFSPSRLLPLIARHLVLNLLWSDHSIDKSQLELWADINHSESRRIHVEDSLTKLFRLNIVEQSSLDSSSSSFDDPRNHHHLTLTLNRSFQQNFRRALTGGGNHCSFGVPCNTSSNAVVSIDELDRYGTEKWETILHYMVGSRLPTKPSHNILSLLGQSGLMTSSDPRSLQSLKITSKGFGFLLEDVNTQLWDILLQYLKMTEVNGLDVVDVLACLFMLGSLELGQEYSFSNWTPTQTQVLQDLVDYGLVLVSAPDRFYPTRLATTLTSTAPPLVSAERAQEEHGFLVLETNYRIYAYTSNPLQIAVLNLFLSLRYRFPNLVVGAVTRESIKSALSNGITADQVIMYLHTHAHPQMRKLEPLLPPTVVDQIRLWELEKNRIRAQEGYLYEDFKSAAEYDSVIQYSRKLGIVLWEHAGLRKLFVGYDGHLTLREFFRRGGASNGNIEQSDSGVNLGDRSSDLGSAWAAPDRLSCKLLHNTRRWSTTLGGSDAWRPPNFSSMDQHQSNHPMRSLLDKTYATSTSTQNIAILSPFVQLSFVFHLAIAQLQRHIQETPDCVVTVIGSSSQAAFQNTLADEHDAVLFTDSSSDCLTKTLLERIEFRFCADLAQLLTLLCELGHSPVERPTLNDDEEDRGMRSRRIGLIITDLTSYLISLPTPTLSDLTRLLGFLSCTQTRLPSIHHHHHHHHHPLQNPPPIYLVDRVDHTQKWPLSTPTADPEDLRNILEIFRHFFAQIWTIDGLHETDSNTGDDSPTTTPAAVGTSSSPKPGRYRLYSLRSAADSSADDDDQITYQVLEQDALDPQYSAHPERLLRKTVVVPGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3K420</accession>
    <name>E3K420_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">LIM zinc-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_04778</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78822.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323241.2">
        <property type="nucleotide sequence ID" value="XM_003323193.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K420"/>
    <dbReference type="STRING" id="418459.E3K420"/>
    <dbReference type="GeneID" id="10536842"/>
    <dbReference type="KEGG" id="pgr:PGTG_04778"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04778"/>
    <dbReference type="eggNOG" id="KOG1700">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054591_0_0_1"/>
    <dbReference type="InParanoid" id="E3K420"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030695">
        <property type="term" value="F:GTPase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09326">
        <property type="entry name" value="LIM_CRP_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000145">
        <property type="entry name" value="Cysteine and glycine-rich protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.10.110.10:FF:000001">
        <property type="entry name" value="Cysteine and glycine-rich protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.110.10">
        <property type="entry name" value="Cysteine Rich Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001781">
        <property type="entry name" value="Znf_LIM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24215:SF35">
        <property type="entry name" value="MUSCLE LIM PROTEIN MLP84B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24215">
        <property type="entry name" value="RHO-GTPASE-ACTIVATING PROTEIN LRG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00412">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00132">
        <property type="entry name" value="LIM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00478">
        <property type="entry name" value="LIM_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50023">
        <property type="entry name" value="LIM_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0440">LIM domain</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="26"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="LIM zinc-binding" evidence="9">
        <location>
            <begin position="259"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="119"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="137"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="180"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="196"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP78822.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="332" mass="36383" checksum="8F66D4B1D53A79D6" modified="2012-04-18" version="2">MSRTNTIHLNHRPSTNTKRAFAPATPKCPACLKAVYAAEQALGPGATPYHKSCLRCTHCKKVLDPFSILEHGYDPYCKICHSKAFGTKGVGFGNAVVGEYPTSPKLQSIVSPTIAFGSSLREERDSPSKPDLPFEPVPDHHHHEPIQAEPKPTNDQEADQEDFEPPSLSSLQIIVKPRPKTSETPEEVLHEDRTTVSRSSSTLHSTRQLSLKQEHPLTPPPKPNTSALSFPARAASSSPYNLRPSPTFPRPGFGADGPQRCPACTQTVYHAEQVLAIGKKWHKRCLRCTVCSKALDSNFNERAGKPYCTKCYDVHFGTGAHGFVLRAGFAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>E3K4G7</accession>
    <name>E3K4G7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ArsA/GET3 Anion-transporting ATPase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_05474</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79153.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323572.2">
        <property type="nucleotide sequence ID" value="XM_003323524.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K4G7"/>
    <dbReference type="STRING" id="418459.E3K4G7"/>
    <dbReference type="GeneID" id="10530976"/>
    <dbReference type="KEGG" id="pgr:PGTG_05474"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05474"/>
    <dbReference type="eggNOG" id="KOG2825">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_272870_0_0_1"/>
    <dbReference type="InParanoid" id="E3K4G7"/>
    <dbReference type="OrthoDB" id="1770at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043529">
        <property type="term" value="C:GET complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071816">
        <property type="term" value="P:tail-anchored membrane protein insertion into ER membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02035">
        <property type="entry name" value="ArsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025723">
        <property type="entry name" value="ArsA/GET3_ATPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016300">
        <property type="entry name" value="ATPase_ArsA/GET3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053094">
        <property type="entry name" value="Zinc_metalloprotease_ZmpB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00345">
        <property type="entry name" value="GET3_arsA_TRC40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193:SF2">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02374">
        <property type="entry name" value="ArsA_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1050"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="domain" description="ArsA/GET3 Anion-transporting ATPase-like" evidence="3">
        <location>
            <begin position="2"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="269"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="458"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="552"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="997"/>
            <end position="1033"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1198"/>
            <end position="1243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="486"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="518"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="557"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="632"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1005"/>
            <end position="1021"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1226"/>
            <end position="1243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP79153.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1243" mass="139259" checksum="813FBE37AACA2FF2" modified="2012-04-18" version="2">MDPAHNLADTFCQKFSKHAMRVSGFENLYGIEIELQGDQDGMAGMMPDPVFTIPGMDEAMRFAEIMKRVQSIKYSVIIFDTAPTSHALRFLSFPSIFENAIEKLSLLSGHLGPIMQQFGSIMGLRSTNADDMFGKLNGMRALIAEVNGQLKDPDLTTFVCVCISESWSLHETARLIQALTSYKFGAHCIVVNQLLYPKPNTDCDQCNTRYKMQWKYLTEIRNRYMEEFHVVPMPLLTEELRGVKSITNFSKMLIAPVLAQQTIVPKVGESSGRAWKKRPAAKATDSMTHEKNTSNETASALAFPTHQSIGRNANVSLESSRKTTRNGAKSDSVISDEDDELRPGQDNAKEGNTFSATRSHETSAPPRIQERELAQARGNGAAQANFDTQDVGHLRHTSTPHVGATSRFSEPILDFDARRRRVNWHELCPRIGPTPTGQAKDLKAPTGQILKVPRTQTDKGWADNAALPQNTQDTVVPSIPAERSEITTLPNKPNNLASHDRSSSQTPAISKPKHINSHDNPLNASTMTSISQMGSANNFIPNSQQLASISKATSNEMEGKHTTQARKVDGNSFQANGTRGVNEADENGLANEVNGIGVQEGRKRKRTSVEDLDSTENNSARDKNPKTLVPVNVSSAHIKLQSTRPGPPKDGALTTDVEREEALTPSSSTVRELPRELPLFLPAPSSSTVRELPLFLPTPSSSTVRELPLFLPTPSSSTVRELPLKDRKQNVQHFWIFKENETSKNVRQGNAEELRMPKDKKGVTRRAIKWIIWKSALQPGVLLHQIWSIRQCEDMRLAHEKMEEDSKNRRWDYGPELMEKVIDMIEWFSSDTKETPQLLQVNCEDPFFQQRIVRLDIIKNAWESTETEKVAYWKALGGMMIRTDENPKYTEDKGAQRIFGPAFKKLHSLSVVAFDQIDKFLGPGKSPSSPEHPKSDTLLVAGEFLIGKIRALQTAVKSSTRTSAIRGPNGLMILQKRIWETLLCVLMMQHSVKFAAEKGRDSRTATDPNRTQETAENSSTYDRGRSLFKSGDVGPNPTKNQLKEWGKIRLSAFGSMAVFFLYGVAGWWSCLIDPWRFNQKDVWSIVQLAHVKNKWIYAPDHLPTQHADETPWYTTDSFVRWLLRETNMACRDMTQIDWEFAPAFWAKHVTELNIARFALEDILNQVCAQNPLKSLNMHGKVSPDVKLDDASRKEMEKIKASISSGWKSTFKSHHQSTSDHQQSTSTHKDSRKGGAEVDLTRGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3K4N2</accession>
    <name>E3K4N2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_05519</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79198.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323617.2">
        <property type="nucleotide sequence ID" value="XM_003323569.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K4N2"/>
    <dbReference type="GeneID" id="10531022"/>
    <dbReference type="KEGG" id="pgr:PGTG_05519"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05519"/>
    <dbReference type="HOGENOM" id="CLU_2723383_0_0_1"/>
    <dbReference type="InParanoid" id="E3K4N2"/>
    <dbReference type="OrthoDB" id="10362728at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP79198.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="72" mass="7823" checksum="CAF499933119115B" modified="2012-04-18" version="2">MANCGIHSFVKMDPSFDLNRPLSSISSDLKDGLNNHVRLLCLQGHPKFTYDILPDSLSAQAKNGYASSNSPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3K4Z2</accession>
    <name>E3K4Z2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Velvet domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_05628</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323726.1">
        <property type="nucleotide sequence ID" value="XM_003323678.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K4Z2"/>
    <dbReference type="GeneID" id="10531131"/>
    <dbReference type="KEGG" id="pgr:PGTG_05628"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05628"/>
    <dbReference type="HOGENOM" id="CLU_1435064_0_0_1"/>
    <dbReference type="InParanoid" id="E3K4Z2"/>
    <dbReference type="OrthoDB" id="5599552at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3960">
        <property type="entry name" value="Velvet domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021740">
        <property type="entry name" value="Velvet"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037525">
        <property type="entry name" value="Velvet_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038491">
        <property type="entry name" value="Velvet_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33572">
        <property type="entry name" value="SPORE DEVELOPMENT REGULATOR VOSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33572:SF3">
        <property type="entry name" value="VELVET COMPLEX SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11754">
        <property type="entry name" value="Velvet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51821">
        <property type="entry name" value="VELVET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Velvet" evidence="5">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP79307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="189" mass="21470" checksum="24CF5F2501633A09" modified="2011-01-11" version="1">MANEFPRFNILQHLARGIRLDEGRFPGGTGQTPVMPCLVARGEVPDGFDTNSVPFEQYECTLQLYDSTGAVRLPDTLMFRLQPVRCQFVPSMEDPNQIDPLFVFQEIMIRDCGVFRLRVILYEIGQTRVIATTMTDPFDIVEYGEFPGGARIWTPLSQHLRAHGIVLHLPPDNDSINWDEEYPTTNGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3K511</accession>
    <name>E3K511_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FunK1 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_05647</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79326.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323745.2">
        <property type="nucleotide sequence ID" value="XM_003323697.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K511"/>
    <dbReference type="GeneID" id="10531150"/>
    <dbReference type="KEGG" id="pgr:PGTG_05647"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05647"/>
    <dbReference type="eggNOG" id="ENOG502S5WB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005513_5_2_1"/>
    <dbReference type="InParanoid" id="E3K511"/>
    <dbReference type="OrthoDB" id="5584477at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040976">
        <property type="entry name" value="Pkinase_fungal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38248:SF2">
        <property type="entry name" value="FUNK1 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38248">
        <property type="entry name" value="FUNK1 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17667">
        <property type="entry name" value="Pkinase_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fungal-type protein kinase" evidence="2">
        <location>
            <begin position="245"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP79326.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="712" mass="80769" checksum="B1A5C58953B12AF1" modified="2012-04-18" version="2">MDQPAIVLVKQPLHISLRNALTSHGINTLEELFSLGKRHTKEHLVLLHAIIRLQIIQSPSDSKSRLVKALPQISSLPENNCLKPFKPLVTLVLASSELKSDQEIYNLAEQLCMSHPSPSVTSTSMKSSTSSSHSSNSPPALKLELMYGNLPGLLEHFLQQHQTDTVSVLKPHDIFINQKYEERISEPSMMLKWIRSLVEYCSACLKNQTPTQVHSRAWACPRKHLKGIKGKRKLDCGIVSQPFKWTNHIEHILVPVKLKEDKSQARDADIDLAKVVCEIFKAQPTRSSVVGLTLCGTFMRLWQFDRSGAIGSELLDIKETKENLFQFLNLIVLFLISNKRVLGFDPSFIESYGPNCTDTPQSLKVQIDAKSSNPQDLVIDRPDSPIFRASGICGRGTTCWRAHLLGDVDQKFVVKDSWQPLDQKAEGDMLCEVTKKKVPHMVRYHHHEDVEVANQRVDIQSHLRAGINFNNCTTLQTIHAPLNQQQQPNGFTNRVHRRLILKDVGQPIWTVDSPVHLLEAFEGCIKGHQALLNAGYLHRDVSITNIMIDKQSDEPDRKSFLIDLDLAIAYPILQEDEEPYTRIGTKIFMSISLLSKQNHHEFVDDLESFFWVLIWVCIHYPAEDRQASCATITGWQDLSPDHLADIKQLHLNQPPRLIAHFTPQYKTFTPLVDCVNRFAKILRGPEVRAKQANELYFDVLTVFQIAQAKKAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3K6G4</accession>
    <name>E3K6G4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EFP79886.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324305.2">
        <property type="nucleotide sequence ID" value="XM_003324257.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K6G4"/>
    <dbReference type="GeneID" id="10533577"/>
    <dbReference type="KEGG" id="pgr:PGTG_05111"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05111"/>
    <dbReference type="HOGENOM" id="CLU_2293050_0_0_1"/>
    <dbReference type="InParanoid" id="E3K6G4"/>
    <dbReference type="OrthoDB" id="2516859at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172414" evidence="1">
        <location>
            <begin position="20"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79886.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="101" mass="11143" checksum="FD5C7455883786B7" modified="2012-04-18" version="2">MHLARSVLFLCSVAATAVAALNGSFLCPEDQHVAWCSVYSWQIQGYAALKPNNNKSSSVLKQRSLIVNSRHLKECVVQQALIPLENQSLKSSSFNKNARLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K6M0</accession>
    <name>E3K6M0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05251</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EFP80026.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324445.2">
        <property type="nucleotide sequence ID" value="XM_003324397.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K6M0"/>
    <dbReference type="GeneID" id="10534043"/>
    <dbReference type="KEGG" id="pgr:PGTG_05251"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05251"/>
    <dbReference type="HOGENOM" id="CLU_744220_0_0_1"/>
    <dbReference type="InParanoid" id="E3K6M0"/>
    <dbReference type="OrthoDB" id="2505225at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="317"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP80026.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="372" mass="42548" checksum="1FF48142DBA10639" modified="2012-04-18" version="2">MISNHIDRLGHIWIEKSRDEDGKTDFPTKKYRVLFQKTCSDLDLGLFLWPQFHVKKIEKALNLGKQSNFVPDTPKIPIYQDNIIELCDQDRLLLFTTEYRSHRVFIDLQISVEFCWASDCDEAEKRGDLKENEGENKCEPLPESSVWIDNLLQWPNPCERYSHYVKVPSSYPDFDVIDEGQDVQQATPSGIVISHTDEQESPEQITIAQTMEPMETNRDTSSNACLFKSTKTIKNQTKCKDKAEQTPTATTHGLLGPTQVKYLTVDSSENPLICNSDPAFSPRKIKILGDKYQDSQTNSLAGEKEIPEKQIQQRLKSTLRQGKENNSNESKKLNSKGKSSGNDKSVKRKSPRLIEMSHSHNQQLSTSKRKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3K7T4</accession>
    <name>E3K7T4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_06139</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="KEGG" id="pgr:PGTG_06139"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06139"/>
    <dbReference type="HOGENOM" id="CLU_1661667_0_0_1"/>
    <dbReference type="InParanoid" id="E3K7T4"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="159" mass="17997" checksum="65115232264F4B5A" modified="2011-01-11" version="1">MGSTACSRQVAVLLRQMDPIRALHPKGESLLSQPAIKLGRSVLPIFKLSRLFFKKLYRENVKLEEVELFTEMCSHQLFCLHSSIEDIIESISMLSFSIRDADRLAPGDTCPAIIQELNKLIELFQPHLSLINLYVLPNMFPNHTATHNAFQAVQSFADT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3K882</accession>
    <name>E3K882_PUCGT</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K882"/>
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    <dbReference type="KEGG" id="pgr:PGTG_06656"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06656"/>
    <dbReference type="HOGENOM" id="CLU_058865_0_0_1"/>
    <dbReference type="InParanoid" id="E3K882"/>
    <dbReference type="OMA" id="HYLRCAP"/>
    <dbReference type="OrthoDB" id="2498244at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP80700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="347" mass="39212" checksum="1883C7045885CFD5" modified="2011-01-11" version="1">MSSSQSHFPGGNPPVGVENVNRAYSTILPNSNSSLSRCISAFVRVLLDIEYNAKKSPSNTWMKTPSAHDFHVGSNLPESIILRPINCIPPGSLLSTSERIAPVFRSIFIHDLSISDFPGVTFAWDHPWDSPWNQIFAKFVLKHWRNGYTSGAFAPFFMNPVEAVNTILQLGILHRWFLGRQKGVRLGQFSHEIKAKKSKSEKKSKIRIQISQHRRETLLKLNVTAETAALFDNIKSTSDTEQIPPRDLLKIPLPWRSEEFCSFAQKLDDIFIDKQSSNKGSRFVHEFVLESRRKTPTSARPAGFKDVPRHLPSNCYAAEYVATLSESQRNLLNPKGAVDLLEIMNIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3K8B0</accession>
    <name>E3K8B0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    <gene>
        <name evidence="2" type="ORF">PGTG_06684</name>
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    <organism evidence="2 3">
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        <lineage>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
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    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80728.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="AlphaFoldDB" id="E3K8B0"/>
    <dbReference type="GeneID" id="10539747"/>
    <dbReference type="KEGG" id="pgr:PGTG_06684"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06684"/>
    <dbReference type="HOGENOM" id="CLU_1807130_0_0_1"/>
    <dbReference type="InParanoid" id="E3K8B0"/>
    <dbReference type="OrthoDB" id="2508260at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP80728.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="143" mass="15901" checksum="8810CDEEB5344D0C" modified="2012-04-18" version="2">MNGSKINNSRPGRTLFGCEHEQHKRRTLGAVVIIRHVQGVLQFTTNSLASDDSVPLELYKRGIAGVTPGQGLLLRQPTSTPDVRRAEASGTTDSMKESRSSFCYTPRMQYIGRQTTVQGHCHATALPPQRHLQSSDYVRVPYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3K8Z3</accession>
    <name>E3K8Z3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PSP proline-rich domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_06514</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
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    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324977.1">
        <property type="nucleotide sequence ID" value="XM_003324929.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K8Z3"/>
    <dbReference type="FunCoup" id="E3K8Z3">
        <property type="interactions" value="136"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K8Z3"/>
    <dbReference type="GeneID" id="10539569"/>
    <dbReference type="KEGG" id="pgr:PGTG_06514"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06514"/>
    <dbReference type="eggNOG" id="KOG2330">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014435_1_1_1"/>
    <dbReference type="InParanoid" id="E3K8Z3"/>
    <dbReference type="OMA" id="KGEPIGQ"/>
    <dbReference type="OrthoDB" id="10260794at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071014">
        <property type="term" value="C:post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007180">
        <property type="entry name" value="DUF382"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006568">
        <property type="entry name" value="PSP_pro-rich"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052584">
        <property type="entry name" value="U2_snRNP_Complex_Component"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12785">
        <property type="entry name" value="SPLICING FACTOR 3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12785:SF6">
        <property type="entry name" value="SPLICING FACTOR 3B SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04037">
        <property type="entry name" value="DUF382"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04046">
        <property type="entry name" value="PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00581">
        <property type="entry name" value="PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PSP proline-rich" evidence="2">
        <location>
            <begin position="298"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="400"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="513"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="403"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="431"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="548"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="598"/>
            <end position="609"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP80558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="609" mass="67355" checksum="1471433AE1B6FA4C" modified="2011-01-11" version="1">MAAVALNGNDPNGIHPKTKGLTRGQLKRLKQKQKPKRPTTHSNINGVSSPKHDSADRSDENKEDDESGDVQSLASSVVDDALLGTGELDNSTRELFADVFAKFQPTDQDTISMEEEKPEDPSKGQVIYSDDEMPSDGDSDAPKPDVSKRKQRKMARLTVAELKRLVKKPEVVEWVDVTAQDPKLLVQLKSYRNTVPIPIHWSQKRDYLQGKRGIEKPAFQLPSYIADTGIATQRDAIKEKESEQSLRQKTRERVQPKMGKIDIDYQKLHDAFFKFQVPPVMTKFGEMYHEGKEFETKLKEKRPGDLSEDLKEALSIPPLAPPPWLIAMQRYGPPPSYPNLKIPGLNAPIPEGAQWGYHPGGWGKPPTDEFGRPLYGDVFGQAPKFDSDAYGEPVSKELWGEVQSDEEEESEEESEEESDGEGAGPSAGDGTQTPTGLETPSGLASITSTVPGGLETPDFIELRKRREGTGAVEAEADSGPKSLYHVIPEKETKITGFMGSDRVYDVRGISQHGHNVSMLGQEDRGTKRKANGVDVALDPSELEGLSEEQLRQRYEESRQRASGGGVGNQQGGREDLSDVVAQESAKRKGRADGSANPKNRDREKEKFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>E3K9F2</accession>
    <name>E3K9F2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Crossover junction endonuclease MUS81</fullName>
            <ecNumber evidence="14">3.1.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PGTG_07324</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Interacts with EME1 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, D-loops, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication fork intermediates. May be required in meiosis for the repair of meiosis-specific double strand breaks subsequent to single-end invasion (SEI).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with EME1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the XPF family.</text>
    </comment>
    <dbReference type="EC" id="3.1.22.-" evidence="14"/>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP81072.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325491.2">
        <property type="nucleotide sequence ID" value="XM_003325443.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K9F2"/>
    <dbReference type="FunCoup" id="E3K9F2">
        <property type="interactions" value="118"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K9F2"/>
    <dbReference type="GeneID" id="10536394"/>
    <dbReference type="KEGG" id="pgr:PGTG_07324"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07324"/>
    <dbReference type="eggNOG" id="KOG2379">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_300734_0_0_1"/>
    <dbReference type="InParanoid" id="E3K9F2"/>
    <dbReference type="OrthoDB" id="5963188at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048476">
        <property type="term" value="C:Holliday junction resolvase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048257">
        <property type="term" value="F:3'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd21036">
        <property type="entry name" value="WH_MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20074">
        <property type="entry name" value="XPF_nuclease_Mus81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000307">
        <property type="entry name" value="Crossover junction endonuclease MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10130:FF:000003">
        <property type="entry name" value="Crossover junction endonuclease MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.670:FF:000013">
        <property type="entry name" value="Endonuclease, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.670">
        <property type="entry name" value="Crossover junction endonuclease EME1, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.110">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027421">
        <property type="entry name" value="DNA_pol_lamdba_lyase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042530">
        <property type="entry name" value="EME1/EME2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010996">
        <property type="entry name" value="HHH_MUS81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033309">
        <property type="entry name" value="Mus81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047417">
        <property type="entry name" value="WHD_MUS81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047416">
        <property type="entry name" value="XPF_nuclease_Mus81"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13451">
        <property type="entry name" value="CLASS II CROSSOVER JUNCTION ENDONUCLEASE MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13451:SF0">
        <property type="entry name" value="CROSSOVER JUNCTION ENDONUCLEASE MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14716">
        <property type="entry name" value="HHH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47802">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10 14" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="11 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 14" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0469">Meiosis</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 14" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC4" evidence="16">
        <location>
            <begin position="757"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="76"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="331"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="509"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="586"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="676"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="15">
        <location>
            <begin position="144"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="184"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="337"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="361"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="372"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="403"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="509"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="588"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="678"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="15">
        <location>
            <begin position="704"/>
            <end position="718"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU369042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EFP81072.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1098" mass="120422" checksum="5192EA2C58CCB9B0" modified="2012-04-18" version="2">MAPSTSLNAEWAGWVQEAADHAANNGSRSCDTYRRAARAIRLHNQPLHHPKQALALNGVGEGIVRLITARLQEKCDATGDPFPTEPESLKGKRKKDTTNPTRPRKRAKEILTAEERALDPSSSEEEEEQEQRPSNNRTGSSAGQKKKGKPRQPRRRRKIEVLTPEERALDPSSSDPEPSLPPKTTRQASPAQPKTRTNQKKYIPKRRTGCWGILVGLSILTTERAGSFTKQEIIEHAQKYCDSSYTERGSRGGGAGNSGHYTAWNMKKLLAEGLSLSTPGRQAKFTLTQQGFELASILAEKEDLTLVPFDKSKLPDGNSMSLLETHVDSARDPLPAASSSKAPVTSTSNASNLNPCGPNPSSSSDTIHSASHQRANTTAQKSNNPQRKNSLPTIPDDPEIACLNTNSSQQSSRVSHNRGPFEFWYLDRAGLRVRNMDDADSRMHPRTFETLYQVEFAASQSSHPVKRSSIVDEAALRPSPMNSSEGHMMGWMYESKAIPSCPGFGLSTHSPTLSTSTTIPPPTLSSSYNKNNSAPHVPSASQRRRSSPSPDPPVNIIHDDSYCGLSAQTTISAGYQNRISPLDRLFKPSSSGSATPAGSVVSIPSEADPAAPKDLAPSARLKPPSRPGSCEILWSGAALSETVAGWSASESPDSLPVSGAVNSLASSRTVVDLSREASNLSHQSSSLDRSSSSCPLRNLKRTTSALSESRSSSDGLPSNRTRVNLKRTPSGLSESLLSRASDDVQPEVWKAGSYDICLVLDHREVRAINDRDAFYNLCVEKVSALASPGQPTIRVLQRALVLGDAIWIAVHRDSQREVVLDSIVERKRLDDLCASIKDNRFEEQKARLKRSGLRDPIYLVEAYNTQSNLDSYGQMIYTSKFETMLLNDFQLEATPDWKASVEFLVRRSEVLRDLHQTIDLSIIPDATIDRTTYLFHLADLRTKDAENSARYWVTTYGAFEVLNHKTATLTVRELWARMAHCLPGMSASKVASFVDHWPTPVSFWADLLAHTSAPLSDQTKADKYPWSWIDRHPNEPLPSALSHPDHPVEMSIASISSTQTSCSAYPANCANSRIGPALSKRLWELYTLSDYASVEPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>E3K9Q2</accession>
    <name>E3K9Q2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Pre-rRNA-processing protein IPI3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_07178</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of the RIX1 complex required for processing of ITS2 sequences from 35S pre-rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the RIX1 complex, composed of IPI1, RIX1/IPI2 and IPI3 in a 1:2:2 stoichiometry. The complex interacts (via RIX1) with MDN1 (via its hexameric AAA ATPase ring) and the pre-60S ribosome particles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the WD repeat IPI3/WDR18 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP80926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325345.1">
        <property type="nucleotide sequence ID" value="XM_003325297.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K9Q2"/>
    <dbReference type="STRING" id="418459.E3K9Q2"/>
    <dbReference type="GeneID" id="10536084"/>
    <dbReference type="KEGG" id="pgr:PGTG_07178"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07178"/>
    <dbReference type="eggNOG" id="KOG0646">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029749_4_0_1"/>
    <dbReference type="InParanoid" id="E3K9Q2"/>
    <dbReference type="OMA" id="AINDEMW"/>
    <dbReference type="OrthoDB" id="756370at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120330">
        <property type="term" value="C:rixosome complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001940">
        <property type="entry name" value="Pre-rRNA-processing protein IPI3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045227">
        <property type="entry name" value="WDR18/Ipi3/RID3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18763:SF0">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18763">
        <property type="entry name" value="WD-REPEAT PROTEIN 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="2 4 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="107"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="142"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="307"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="416"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="479"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="324"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="416"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU369067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP80926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="572" mass="60309" checksum="4FF9FD999265B1BA" modified="2011-01-11" version="1">MSTSAILFSGLPNSSEGTQAITFHELSTSSQVHQLKSPANAAFGLSSTVQNAGLPPRKTLACLPSTNSLGSTVLTISGKDGRNGILLWSTLAGKPTPSHRLIPPGRVIVVALSPSGTYLASGSADGVIMLWELSTGTLLASFDGHYKSVTCLAFSDDEAALVSASEDSMCSVWSIPTLIDEAFDSNLSHTPYSIFSDHTLPISDLFISKGCFPDLRIFTASLDQTIKIWSPLYPSSPLLSTFAVPGPAHHLAVDPLERFIIVSYATMLKNESQEPQQANGTASQPQQSNTIPASTLKIIPLFSRPSHAAPQGGVSRVHGPSGLVQRTESGNEEVSYTSPRSTQITALHLPSPIVSSSVVLCGLSNGKVVHLSMPSLQPLGQTVPHPNAAGEAKDPVVYLSTFARPADLLSSSVADALSGPDHSGSSSVSPRPVGVLGRVIGKNGDPQRGSVPLQAGQPQRACRELCTMLKIEQVDEHALNQDDPFSLPSREPRPDSTSSALHSLPDHSLVHSHQAAVGDHQLKSQTDSIEKLTAETVHLKAQLKQALAFNDSLWAGIVDGTLAFKESALPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>E3K9Z9</accession>
    <name>E3K9Z9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Importin-95</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Karyopherin-95</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_07233</name>
    </gene>
    <organism evidence="11 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the importin beta family. Importin beta-1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP80981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EHS62969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325400.1">
        <property type="nucleotide sequence ID" value="XM_003325352.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890052.1">
        <property type="nucleotide sequence ID" value="XM_003890003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K9Z9"/>
    <dbReference type="FunCoup" id="E3K9Z9">
        <property type="interactions" value="979"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K9Z9"/>
    <dbReference type="GeneID" id="10536220"/>
    <dbReference type="KEGG" id="pgr:PGTG_07233"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07233"/>
    <dbReference type="eggNOG" id="KOG1241">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008296_1_0_1"/>
    <dbReference type="InParanoid" id="E3K9Z9"/>
    <dbReference type="OMA" id="QQYQERW"/>
    <dbReference type="OrthoDB" id="10263328at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000027">
        <property type="entry name" value="Importin subunit beta-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058584">
        <property type="entry name" value="IMB1_TNPO1-like_TPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25574">
        <property type="entry name" value="TPR_IMB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="10">
        <location>
            <begin position="21"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="9">
        <location>
            <begin position="410"/>
            <end position="440"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00079884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00083566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP80981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS62969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="861" mass="93513" checksum="754216A09231D663" modified="2011-01-11" version="1">MDAATLLANTLSADLALRTAATTQLETASREHPAPYLDSLLGVLTSVDQPAHIRNAAGLALKNALTSRDAGRNEELIERWKSITDELRLKIKDGLIRLLADEQRAVRQVSGQAIAAIGAVELPLGMWPGLIGQLLQIINNAEGGVPLRQATLQAIGYLCESTLPEVLAAQSNEILTAVVSGARKEEPSAEVQLAAVNALYNSLEFVRANFDREGERNYIMQVVCEATQSPTPDVQVAAFGCLVKIMQLFYDKMRFYMERALFGLTVLGMKHADERVVLQAIEFWSTVCDEEIELQIEAEEALEYSEPTERECQHFAKVALPEILPVLLQLLTRQSEDADEDEWNVSMAAGTSLALLAQTVGDAVVAPVIPFVESNIKSADWHQRDAAIMAFGSILDGPDPKMLDPLVSQALPTLIEMMQDPSMHVKDTAAWTLGRVTDQLIGTIKPDVHLEPLITVLLGGLGDNTRIVGNCCWGLMNLAEQLGDPSKPDSVLSRFYEGIANSLIAFSDRVADEPTSRTSAYEALGTLMKFASPEALPVVSNALVVIMERSERLLGMQAQLVGDDDRRNYADLQVAFCAVLTHAIRRLGPEVKPMADRAMTLILRLIQGAGPKSPILDDAFLAVGAMTYALETDFQAYLPAFLPFLNAALKSHEEYPLCCIGVGIVGDICRGLGEQAAPFAQGFMELLLEDLQSTILHRSVKPPILSCFGDIALAVGGNFAPYLETTIGVLQQAGAMRADPTNYDLVDYINQLREGILEAYAGIIAGLKSANKTDLILPHVPTICGFLHVVVTDQDQTEELLKGALGIIGDLAEIFSSGQIKGALDQQWIAEALKTGRTRVGGPETKKLAKWAKEMVRRATQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAN6</accession>
    <name>E3KAN6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81443.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325862.2">
        <property type="nucleotide sequence ID" value="XM_003325814.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KAN6"/>
    <dbReference type="GeneID" id="10537181"/>
    <dbReference type="KEGG" id="pgr:PGTG_07064"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07064"/>
    <dbReference type="HOGENOM" id="CLU_983982_0_0_1"/>
    <dbReference type="InParanoid" id="E3KAN6"/>
    <dbReference type="OrthoDB" id="2506988at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF5">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81443.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="283" mass="31233" checksum="072F62BC76682CF8" modified="2012-04-18" version="2">MAQPINPPVPLTPPNSSNNPVDHPQSVRRESTRLRTPSLRPGFISTQGDSCRALVPSSPAISTNRSQVQAQKKKKNVVNIVEDDGESDSDTNFPPNSRSKSPTNLLAGTKQVASRTGIEVIIDHAQDSDLENERHTKTRKKKDPTKDKDGFDHARLFFYFPGSGPKQNPNGTAWACRWCPNEFKASGGSYYNLKSHRDGANIKGSLRSACPGRGKAIKEGAHLPPTAAEEKKTRAAGEGTLLALWSLFLCMKEENSKFYYLSMKDSRYGQMDVRNAIYGQFNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAP4</accession>
    <name>E3KAP4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07072</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325870.1">
        <property type="nucleotide sequence ID" value="XM_003325822.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KAP4"/>
    <dbReference type="GeneID" id="10537189"/>
    <dbReference type="KEGG" id="pgr:PGTG_07072"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07072"/>
    <dbReference type="HOGENOM" id="CLU_990909_0_0_1"/>
    <dbReference type="InParanoid" id="E3KAP4"/>
    <dbReference type="OMA" id="QIAGTHE"/>
    <dbReference type="OrthoDB" id="2510501at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173695" evidence="1">
        <location>
            <begin position="21"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="281" mass="27854" checksum="47D29EE1F810EF43" modified="2011-01-11" version="1">MHVSCSVISAAILLAGQSQSAPILGLDDGLLSTNLQLLGGLTHTIEGVPVAGQLTNGLLGVSNDGTSAGLLTTHGPDGGSKLAEVLEASKPDSLVEGSKSGGLLTVSGKDGSDSLLDTHGLFGKSTLRKRFSLPLIGEVAVFDGFTTKAKSMASDLGSKLSGILRKRSTQPLPSPTSLLSNLPVVGYIPGMPALPAISSLPVVRNIPGLALIPGLGTTSAASGIGAAVNSIASVHRSATEAALDAPMNTINTGINSAFLGAVGAVSGAPAAVDSLLNKPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3KB12</accession>
    <name>E3KB12_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF7137 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_07987</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326157.1">
        <property type="nucleotide sequence ID" value="XM_003326109.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KB12"/>
    <dbReference type="STRING" id="418459.E3KB12"/>
    <dbReference type="GeneID" id="10533446"/>
    <dbReference type="KEGG" id="pgr:PGTG_07987"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07987"/>
    <dbReference type="eggNOG" id="ENOG502S1CS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078019_0_0_1"/>
    <dbReference type="InParanoid" id="E3KB12"/>
    <dbReference type="OMA" id="WGWNYTN"/>
    <dbReference type="OrthoDB" id="2435509at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055561">
        <property type="entry name" value="DUF7137"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42028">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42028:SF1">
        <property type="entry name" value="YALI0E30657P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23585">
        <property type="entry name" value="DUF7137"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005673254" description="DUF7137 domain-containing protein" evidence="3">
        <location>
            <begin position="24"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="265"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7137" evidence="4">
        <location>
            <begin position="117"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="34"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF23585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP81738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="289" mass="31225" checksum="3F60B975A522C415" modified="2011-01-11" version="1">MRLILLRTICILITTSIIPPTLNQLINQPSNNQLQPRQVVNNPNQNQNQNQNPNQNQNANQNQNQNQNQNQNANQNQNQNQPTTTAGQVQATQSASRAGTNAPGPSTSSVFSAPIDSPAGGLVFTQPPATAAASYYKIAQNQPLTFGWNYTNILSYGNSITVVAYCSLNKISYTISTLPAQATAITWDPYALQQQPGYPLFAEATYTLQIFDERGMGAGPTPGLMAPSSQMKFAMYKPQPYTPLASWTCSTCSGARGFFHNYSPVLLACSLSLITAIGGGWYGVLAAIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3KB16</accession>
    <name>E3KB16_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07991</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81742.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326161.2">
        <property type="nucleotide sequence ID" value="XM_003326113.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KB16"/>
    <dbReference type="GeneID" id="10533450"/>
    <dbReference type="KEGG" id="pgr:PGTG_07991"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07991"/>
    <dbReference type="HOGENOM" id="CLU_032241_1_0_1"/>
    <dbReference type="InParanoid" id="E3KB16"/>
    <dbReference type="OrthoDB" id="2507728at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="332"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="404"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="216"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="273"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81742.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="450" mass="49936" checksum="F22E661B22622B63" modified="2012-04-18" version="2">MDIPLPSAQDESTEPKRRRTKPEMIIHRAELAKKKEALEAAKLLKKKPHPKAVRTAAGTSGSKSTKPTGAADNNPQFIASDFELICTYLEDPHHYTEIYGNGPRTGVGQQVMTKSAAYDRFAIFMNDKTHKRLHLDGKLLRQRIEAYKKRFVAAKRWADNTGVGIEEGEDIVSIDVLLEGKCPCYKRMSALFGEKPNVTPAAQYESQQGMSLYNQPVDNDNPENPSNPEMFYPGWEPTQEVLDQTTHVNKSPDLATSGQANQTSGVDQSNENPDLPDSLLDAPPSHQPTPALDSSPVSAMPSTSATPGRQLGLSQPRRTMASMLADLRANISREEEDEVDDAEPSGSAAPRGRFVNQVDRSIPAPRRETNNPKGKSTVAAAFGSSSDKKFAYLDKHMEMEKEKFEWEKSKHKSEQDDITAREKAKMRAAEKWISQGKSPSDIEILMRTVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCP5</accession>
    <name>E3KCP5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07594</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326616.1">
        <property type="nucleotide sequence ID" value="XM_003326568.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCP5"/>
    <dbReference type="GeneID" id="10529569"/>
    <dbReference type="KEGG" id="pgr:PGTG_07594"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07594"/>
    <dbReference type="HOGENOM" id="CLU_2224502_0_0_1"/>
    <dbReference type="InParanoid" id="E3KCP5"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="67"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="106" mass="11322" checksum="326367DD24B62F90" modified="2011-01-11" version="1">MTLLHKSLRNIKEICCTSAVTSIQSSFGKHSQTSLYKLFRSAEITSTARNDPQSRSALNGDCGPLPSETSSMSDTPNHICSVARSFPVVEMVQAGSRSSSQALLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCQ9</accession>
    <name>E3KCQ9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Cryptochrome DASH</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_07608</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">May have a photoreceptor function.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate</name>
            <dbReference type="ChEBI" id="CHEBI:15636"/>
        </cofactor>
        <text evidence="6">Binds 1 5,10-methenyltetrahydrofolate (MTHF) per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5 6">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="5 6">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the DNA photolyase class-1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326630.1">
        <property type="nucleotide sequence ID" value="XM_003326582.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCQ9"/>
    <dbReference type="STRING" id="418459.E3KCQ9"/>
    <dbReference type="GeneID" id="10529583"/>
    <dbReference type="KEGG" id="pgr:PGTG_07608"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07608"/>
    <dbReference type="eggNOG" id="KOG0133">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010348_6_2_1"/>
    <dbReference type="InParanoid" id="E3KCQ9"/>
    <dbReference type="OMA" id="KFWRCGP"/>
    <dbReference type="OrthoDB" id="435881at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003904">
        <property type="term" value="F:deoxyribodipyrimidine photo-lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000719">
        <property type="term" value="P:photoreactive repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.579.10">
        <property type="entry name" value="DNA Cyclobutane Dipyrimidine Photolyase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014133">
        <property type="entry name" value="Cry_DASH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036134">
        <property type="entry name" value="Crypto/Photolyase_FAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036155">
        <property type="entry name" value="Crypto/Photolyase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005101">
        <property type="entry name" value="Cryptochr/Photolyase_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002081">
        <property type="entry name" value="Cryptochrome/DNA_photolyase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006050">
        <property type="entry name" value="DNA_photolyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02765">
        <property type="entry name" value="crypto_DASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455">
        <property type="entry name" value="CRYPTOCHROME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11455:SF22">
        <property type="entry name" value="CRYPTOCHROME DASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00875">
        <property type="entry name" value="DNA_photolyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03441">
        <property type="entry name" value="FAD_binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00147">
        <property type="entry name" value="DNAPHOTLYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48173">
        <property type="entry name" value="Cryptochrome/photolyase FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52425">
        <property type="entry name" value="Cryptochrome/photolyase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51645">
        <property type="entry name" value="PHR_CRY_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0157">Chromophore</keyword>
    <keyword evidence="3 5 6" id="KW-0274">FAD</keyword>
    <keyword evidence="2 5 6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Photolyase/cryptochrome alpha/beta" evidence="8">
        <location>
            <begin position="7"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="556"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="556"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="313"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="326"/>
            <end position="330"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="366"/>
            <end position="373"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="488"/>
            <end position="490"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602081-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP82211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="596" mass="68644" checksum="0A10BDFA59F2D9AB" modified="2011-01-11" version="1">MGVGPRSLTIALFRNDLRLHDNPILTHSHLATVKEGDAVRRNKVSEYVLPLYVFDERQIELSGLEGYRQHGGPARTEVCGFWRTGSHRLNFLCQSVYELKHQLKKSGSDLLVRFGVVEATTLKIIEELQRNGFSVDHVYMAKEVAFEEVGTEKRLAKLLGELAHKVPLTLFHSRSLVHPDDLPFTINKTPDVYTPFRSKVESLPADQLCRPLLPLPEKLQPFPALPETILKAAPEPGYSGSLCEGQGFDEVFARLVKPLLSNPDIPHDPKEANTQDYKPDPRSAFPYQGGESEALRRLDDYFFKGNQPPVRSYKTTRNGLLGHQYSTKFSPFLAFGCISPRKIIHSLWDHEAKFGSNKDTYWVLFEILWRDYFIFISQKFGRRLFLIKGFEGRIDPKGATKKLSYWKPFKDEKMNQQSKGKETPVDRNALAWLRGKTGVPFIDANMIELRESGFMSNRGRQNVASFLAKDLELDWRIGAEWFESALLDYDPCSNYGNWQYVAGVGNDPRTSRQFNPIKQAKDYDSQGDYVKHWIPDLKSFPTNRIQSPWLMNSQEWDKHLGSSTKEDKENKTGQKDDYPRRPILEQQAWKKHYQRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCR5</accession>
    <name>E3KCR5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_07614</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82217.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326636.2">
        <property type="nucleotide sequence ID" value="XM_003326588.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCR5"/>
    <dbReference type="GeneID" id="10529589"/>
    <dbReference type="KEGG" id="pgr:PGTG_07614"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07614"/>
    <dbReference type="HOGENOM" id="CLU_1876406_0_0_1"/>
    <dbReference type="InParanoid" id="E3KCR5"/>
    <dbReference type="OrthoDB" id="10315934at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP82217.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="136" mass="13914" checksum="E80E3F85C7ACC538" modified="2012-04-18" version="2">MASVKATASSATHGSLGSATAALKRNMPKSIAVPTVLRCPSPSSIVHTATLMTWASAVWPQPQNAADVSSQSSPSASAALLVRPLIPGSPDIAHTVWAKVALKPSCLANTETSMAAGSFSDIDCNNSTTSLKNEPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDG5</accession>
    <name>E3KDG5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">mannan endo-1,4-beta-mannosidase</fullName>
            <ecNumber evidence="4">3.2.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_08357</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random hydrolysis of (1-&gt;4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.</text>
            <dbReference type="EC" id="3.2.1.78"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.78" evidence="4"/>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326820.1">
        <property type="nucleotide sequence ID" value="XM_003326772.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDG5"/>
    <dbReference type="STRING" id="418459.E3KDG5"/>
    <dbReference type="GeneID" id="10529779"/>
    <dbReference type="KEGG" id="pgr:PGTG_08357"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08357"/>
    <dbReference type="eggNOG" id="ENOG502R245">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032232_2_0_1"/>
    <dbReference type="InParanoid" id="E3KDG5"/>
    <dbReference type="OMA" id="GWESGNE"/>
    <dbReference type="OrthoDB" id="406631at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016985">
        <property type="term" value="F:mannan endo-1,4-beta-mannosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000192">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045053">
        <property type="entry name" value="MAN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31451">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31451:SF39">
        <property type="entry name" value="MANNAN ENDO-1,4-BETA-MANNOSIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26410">
        <property type="entry name" value="GH5_mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="310"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="359"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="10">
        <location>
            <begin position="43"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF26410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP82401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="495" mass="55611" checksum="F267633F31E2AB1D" modified="2011-01-11" version="1">MSKLDPNNCFSAQNITRFVSILDGPQAVNRQLGRLDKSPDPGFIRKNGHHLVDGDGHTPFKIVGPNIYWLGLDENVSPSPSYPSQTRVLEAFATAAIMGSTVVRATTLGISVGNPYSVWPTRNNTNNDALDVISFAIYAAKRYGLRLIIPITDQYDYYHGGFKTFLKWRSIPDSDYRSFYDIKSDVYGDFLLYLETLFNHVNRYTQLAIKDDPTIMMWETGNELDNPSKAWTEAIAKWIHTKAPNHLVASGRYGVSTDDLKISAIDAVTNHFYPPRADHYHADAALAASHGKVYYAGEFDWSGRSKSWGLLGWILIPIFLGLLALLIGGCGKIFPIIFEWTTHKAQKPGQTKPQRHRSLIIRQSHVAIFMIFIISPITAGIIYACSIRGTGIEAFLTETELPDSDGSGDLFWSLFGHDDRCCDWVDHPDGFTMHFPGRNDDERGSVAKIMQHSYTMRRQAFNPKNNLNSKIEGDNHDWFGIGDRVLIKCPQSLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDQ0</accession>
    <name>E3KDQ0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08442</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82486.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326905.2">
        <property type="nucleotide sequence ID" value="XM_003326857.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDQ0"/>
    <dbReference type="GeneID" id="10529865"/>
    <dbReference type="KEGG" id="pgr:PGTG_08442"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08442"/>
    <dbReference type="HOGENOM" id="CLU_1156875_0_0_1"/>
    <dbReference type="InParanoid" id="E3KDQ0"/>
    <dbReference type="OrthoDB" id="2508133at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171927" evidence="1">
        <location>
            <begin position="23"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82486.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="240" mass="26531" checksum="1FA54AAB1C7EB882" modified="2012-04-18" version="2">MTAYKFFLTVISLLAYISLCRGQRCFPKGKTSQIACFEAWANINTTDDNTILAGETKVYSSSKSCLVEVISKNTGPTQRSDMYDAMVEVENKCSPAMVGTLSYPHFDISLTQVLPNTPIFGRNIKLDQPSCNIKSPFSSGKISPPDCSKAIDTIQINSKSPQKILSQSKGEIRASFKTCFVFIKSSNQLDITMNHDQLKKMSSLITGKCKEQTGSVVKQFGSFGLNSRTEMQIYFHGKPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDV2</accession>
    <name>E3KDV2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_08292</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326755.1">
        <property type="nucleotide sequence ID" value="XM_003326707.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDV2"/>
    <dbReference type="GeneID" id="10529703"/>
    <dbReference type="KEGG" id="pgr:PGTG_08292"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08292"/>
    <dbReference type="HOGENOM" id="CLU_1441698_0_0_1"/>
    <dbReference type="InParanoid" id="E3KDV2"/>
    <dbReference type="OrthoDB" id="10285121at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP82336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="191" mass="22349" checksum="5F651DF5336370CB" modified="2011-01-11" version="1">MLINKKLTERPEESEVPEPALPDNRVVIPSIDLHYCLYVNSDIIYYRRPRYDDEEATINQGDYQRLMSFGHIEPLEVDLDRTDFESFKSQVLLQLANTRAAYQLGMLIEHFESTRDILWKAQVYSYDERRIVANYDLHERFRSWKYDVLTSPLDLHLSVMILMDHPDASDDPDDLVSLLRNVHDGGVQGFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>E3KE31</accession>
    <name>E3KE31_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome b5 heme-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_08781</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the cytochrome b5 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82585.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327004.2">
        <property type="nucleotide sequence ID" value="XM_003326956.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KE31"/>
    <dbReference type="STRING" id="418459.E3KE31"/>
    <dbReference type="GeneID" id="10529968"/>
    <dbReference type="KEGG" id="pgr:PGTG_08781"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08781"/>
    <dbReference type="eggNOG" id="KOG0141">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0537">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_4_4_1"/>
    <dbReference type="InParanoid" id="E3KE31"/>
    <dbReference type="OrthoDB" id="2588832at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.120.10">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050741">
        <property type="entry name" value="Acyl-CoA_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036400">
        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083:SF28">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G10880)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083">
        <property type="entry name" value="MEDIUM-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3 9" id="KW-0349">Heme</keyword>
    <keyword evidence="8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="10">
        <location>
            <begin position="21"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP82585.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="500" mass="55090" checksum="1079D9E09669AC57" modified="2012-04-18" version="2">MGSQANSFSTVFDTIPSGKQLKSLTREEVAKHDKEGDLWCIIDTAVYDLSKFVDLHPGGASVLVDKNVAGKDATKAFFGLHRSDVLKKYGRYVIGRIEGEKSAVILPKDGELSLVPHAEPSWLSKGYSSPYYTDGHREFQKAMRLIFDKHITPEAREHELNGKRVSQELVDLMAKEHINAMRLGPGKHLHGLTLPAGVKGEDYDYFHELIITQELGRCGARGFSDGLLGGMVIGLPPVLNFGSPELKKKIIPEVLSGKKYIALAISEAFAGSDVAGLRCTATKSADGQHYIVNGTKKWITNGTFADYFSTGVRTDKGLSVLLIERGEGVETKPIKTSYSPAAGTAYITFDNVKVPVANLLGKENKGLQVILSNFNHERWIMCCGVIRASRFITEECFKWTHQRVVFGKPLIAQPVIRNKIAQMFAKCEATQSWLENVTFQMNRMSYAQQADLLAGQIGLLKAYSTRVAHEVADEAVQIFGGRGITRYWNGSLCGDVSTQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KE48</accession>
    <name>E3KE48_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_08994</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82798.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327217.2">
        <property type="nucleotide sequence ID" value="XM_003327169.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KE48"/>
    <dbReference type="GeneID" id="10544505"/>
    <dbReference type="KEGG" id="pgr:PGTG_08994"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08994"/>
    <dbReference type="eggNOG" id="ENOG502T1DR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_199246_0_0_1"/>
    <dbReference type="InParanoid" id="E3KE48"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP82798.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="64" mass="6856" checksum="7D407193DD369F7E" modified="2012-04-18" version="2">MPAQTVQNPSALIGNVPVLNGNSVTFPAWRTQLEDLLAIQGVHDIVTGKLSQPETDYKDAKPVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KFA6</accession>
    <name>E3KFA6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_09901</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178284">
        <property type="protein sequence ID" value="EFP82933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327352.1">
        <property type="nucleotide sequence ID" value="XM_003327304.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KFA6"/>
    <dbReference type="GeneID" id="10544644"/>
    <dbReference type="KEGG" id="pgr:PGTG_09901"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09901"/>
    <dbReference type="HOGENOM" id="CLU_046892_0_0_1"/>
    <dbReference type="InParanoid" id="E3KFA6"/>
    <dbReference type="OrthoDB" id="2509192at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173306" evidence="2">
        <location>
            <begin position="21"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP82933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="466" mass="53145" checksum="77D2ADA661C6D939" modified="2011-01-11" version="1">MSKSLLELVLISLLIGSALLTPPTLPTEKKIIKCSNGKELPDLNMPFADDSLTWSGNPTSDRLSATSAESSSRPRRILNGQNPSSSGKPKDDELSSNRILPRPIDSYISNEPLVSRQSQQHIPSELEHQSAQNFEPDVEGKLKRKRLTTESLQTVSNSKTTRNDHPTEGDKLIPSFFNVYDWDFVKVQDPEELAINSQKPYYGLGDFFTTLNFQRNGNFYFIDVNELANVITTFGENRFLLPRMVKTKNRPKIVGEILLKLANEKLNLEAYKVFSDSFLKHMRARLELRNMIHKESNKRYVTPKKINTISNFISNVTKISTFLIIMRLSLFKQHAEGGLNQKTVERIVMSIKSVWDAMEVKNIQKPQWAERFSMLLRLEEQNPLENEHPIYGSDKMLQMATRIVEHWAEENGSAGYAYKSTKGGNHQQLAEILSNIIIYANPNFVSKFNQSLAKKNKSKLKKTKPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3KG86</accession>
    <name>E3KG86_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_09134</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MIX23 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EFP83181.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327600.2">
        <property type="nucleotide sequence ID" value="XM_003327552.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KG86"/>
    <dbReference type="FunCoup" id="E3KG86">
        <property type="interactions" value="184"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KG86"/>
    <dbReference type="GeneID" id="10544894"/>
    <dbReference type="KEGG" id="pgr:PGTG_09134"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09134"/>
    <dbReference type="HOGENOM" id="CLU_1062224_0_0_1"/>
    <dbReference type="InParanoid" id="E3KG86"/>
    <dbReference type="OrthoDB" id="5593818at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019171">
        <property type="entry name" value="MIX23"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31905">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31905:SF2">
        <property type="entry name" value="PROTEIN MIX23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09774">
        <property type="entry name" value="MIX23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="164"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="243"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00024204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP83181.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="262" mass="29335" checksum="87DBF7111591DCB7" modified="2012-04-18" version="2">MPPKRTTSIKPFKKTTMTAGDRSSSPPDISRPARSSPAWLGTEWLHAQLKTGPPTDPAPQQQQSIIERRLPSSVCLDISEFRRLLQRYRKSDDSITLALNRSFLLLGSPSDHPPHQHNQSQPSTSQCAEFWKILTASWASRENLIRSCIDIVDQSVSERSRAIQELSGAPTKSEQVGRTSGAFEDDIARRHRPSTGEKKRAELEAEVYTAELKRRMIHDELAVESAIRNQALQKFSARCSPSIFPVPDTSPQVNLDPHPVPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGY8</accession>
    <name>E3KGY8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Enhancer of mRNA-decapping protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_08749</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">P-body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EDC3 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327982.1">
        <property type="nucleotide sequence ID" value="XM_003327934.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KGY8"/>
    <dbReference type="STRING" id="418459.E3KGY8"/>
    <dbReference type="GeneID" id="10532247"/>
    <dbReference type="KEGG" id="pgr:PGTG_08749"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08749"/>
    <dbReference type="eggNOG" id="KOG2585">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_500660_0_0_1"/>
    <dbReference type="InParanoid" id="E3KGY8"/>
    <dbReference type="OMA" id="SESCWIS"/>
    <dbReference type="OrthoDB" id="10030313at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031087">
        <property type="term" value="P:deadenylation-independent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033962">
        <property type="term" value="P:P-body assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10260">
        <property type="entry name" value="YjeF N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025762">
        <property type="entry name" value="DFDF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019050">
        <property type="entry name" value="FDF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004443">
        <property type="entry name" value="YjeF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036652">
        <property type="entry name" value="YjeF_N_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13612">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13612:SF0">
        <property type="entry name" value="ENHANCER OF MRNA-DECAPPING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09532">
        <property type="entry name" value="FDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03853">
        <property type="entry name" value="YjeF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01199">
        <property type="entry name" value="FDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64153">
        <property type="entry name" value="YjeF N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51512">
        <property type="entry name" value="DFDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51385">
        <property type="entry name" value="YJEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DFDF" evidence="7">
        <location>
            <begin position="220"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="YjeF N-terminal" evidence="6">
        <location>
            <begin position="344"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="72"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="136"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="563"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="98"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="136"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="161"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="200"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="578"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP83563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="618" mass="67942" checksum="74DBD0D75E89B427" modified="2011-01-11" version="1">MSSPFIGLPISAELSNGTVVEGIIHDIDPSTGKLNLAQARFVKYDGSFSEIQPSFILEKDFVKDLMLLSTTRQTSSSSSSSSSPTQNNNSHRHHPHRQNNNNTNSSSSSNGIQSRLNDLSIDQSNQKTHHHQDLFTNTQVTLQPTNHQKNPQPPSRRRRKERDQPKKQQPDEQANHHHFTSHPFNNNPPDSAHEDTDGIPTPELSPPLTTTSQPQTSESPPAIVPSDFNQDFDFQAGLMAFDKAKLWAEIASTDSTDPKDRLIAHNRKKTNGVHFAQSRRGEEDHPRHRNLGPTEMVLSAQEQVTNGSTVSNPWLQSSNNNNPSNPYILSTDGHPIYPVTFDRLNLALSAASVEYGPSLIQRIENAGRSMTDYIIKLLRQSLSSPKSYKDSTISILVGSHGTKASCAIRTGALLALKGFKVFLVLSTNSNRKPAKARNDAFEFQLRLFSSTGATICPQIRDLPSSPTLIIEALAENSVTSLPNHMIIDKTLIDYTHSAPTLGIDISSYLNYDTGEPLVGTADLPSHQHLVCLGALPKGVLNSPSTPTEVCLIDLTLSESCWRNSADKDSDSDEHDQAGSYTHPSNGNHHAQNGVLSDRLPDLPPASWGDGWYTILKIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHD3</accession>
    <name>E3KHD3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09421</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328127.1">
        <property type="nucleotide sequence ID" value="XM_003328079.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHD3"/>
    <dbReference type="GeneID" id="10532395"/>
    <dbReference type="KEGG" id="pgr:PGTG_09421"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09421"/>
    <dbReference type="HOGENOM" id="CLU_1403075_0_0_1"/>
    <dbReference type="InParanoid" id="E3KHD3"/>
    <dbReference type="OrthoDB" id="2506018at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="194" mass="21358" checksum="7AB992465EC9BD37" modified="2011-01-11" version="1">MPPPPIPDPPPLPTGQVSHEPQTTDSPLKILSEITKFLNEALSPKNPARILNENQIQALLDQTIKLKSAMNIESHLEKITERLERLEQRPLTVSPPTPSTWPSATTTNNKKTNPTINKQGKWTPMIPSNAQINEFKKSSLVICTPPGFVALDSATATDITTKINKILISIDAKIDSHQIEIDGIARLPSKDLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJ38</accession>
    <name>E3KJ38_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_10033</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178290">
        <property type="protein sequence ID" value="EFP84313.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328732.2">
        <property type="nucleotide sequence ID" value="XM_003328684.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJ38"/>
    <dbReference type="GeneID" id="10545655"/>
    <dbReference type="KEGG" id="pgr:PGTG_10033"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10033"/>
    <dbReference type="HOGENOM" id="CLU_039761_2_2_1"/>
    <dbReference type="InParanoid" id="E3KJ38"/>
    <dbReference type="OrthoDB" id="5946233at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058913">
        <property type="entry name" value="Integrase_dom_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177:SF1">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24764">
        <property type="entry name" value="rva_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="221"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP84313.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="516" mass="59646" checksum="15FFD4455AAC8CFD" modified="2012-04-18" version="2">MWRKQQHTIKFSTERTPKDLLIVTPYSSQAKMNPALPSFGDEFQSEVTDLFESGLTDKEVIKFLEEIHSISVSPRTLARRKEDWGLVHHASQQIDQLDEHIKTYFDKGLTYSQIHHALSTTHGYTHSKRTLERKLKAMDLSRRLDDIDMEKVDVDTVVTCMMEIHQTPEGRNAGYRKMRQMLQTNFGINVHHMTVALINQTLDPDGVENRAKRVLKRRVFNTPGPNFIWSADGHDKLKKFGITLYGFIDAWSRKILGIFVHVTNNDPRHVGYYYLQLVKREGGIPRRTTTDRGTETIQMAGHQINLTEHYNPHCLDSSTSHLFTKSTHNQKIECLWSQLMKQFNSELINQLFSAAEEHLYDPEDPLEQLVFLYLWVPLLQEALNEWTTNYNSYKRRLDKKSSLPTQCSADWCYAYPDRKGGIQGLIPVPPSAVKTLEEAFYPEGPQLMQTSPTWFYETIEQLQSGLGLVIPNVNVHNVWEVFSLILTAVNTYDEAWLIDPSNDESQTIRARSHLPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>E3KL26</accession>
    <name>E3KL26_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="13">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PGTG_11170</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:21249"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="13"/>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP85001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329420.1">
        <property type="nucleotide sequence ID" value="XM_003329372.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KL26"/>
    <dbReference type="FunCoup" id="E3KL26">
        <property type="interactions" value="353"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KL26"/>
    <dbReference type="GeneID" id="10526978"/>
    <dbReference type="KEGG" id="pgr:PGTG_11170"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11170"/>
    <dbReference type="eggNOG" id="KOG0216">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000524_5_1_1"/>
    <dbReference type="InParanoid" id="E3KL26"/>
    <dbReference type="OMA" id="FFGVVHY"/>
    <dbReference type="OrthoDB" id="10248617at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006363">
        <property type="term" value="P:termination of RNA polymerase I transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006361">
        <property type="term" value="P:transcription initiation at RNA polymerase I promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.270.10:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.270.10:FF:000011">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.150:FF:000004">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1070.20:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1100.10:FF:000008">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1100.10:FF:000016">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1110.10:FF:000007">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1800.10:FF:000008">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1800.10">
        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="entry name" value="RNA polymerase Rpb2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009674">
        <property type="entry name" value="Rpa2_dom_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06883">
        <property type="entry name" value="RNA_pol_Rpa2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 13 21" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <keyword evidence="7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="18">
        <location>
            <begin position="52"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="17">
        <location>
            <begin position="217"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="19">
        <location>
            <begin position="491"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase I subunit RPA2" evidence="20">
        <location>
            <begin position="597"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="15">
        <location>
            <begin position="704"/>
            <end position="1072"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="16">
        <location>
            <begin position="1074"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF06883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="EFP85001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1224" mass="136175" checksum="9777B9F4E6546021" modified="2011-01-11" version="1">MSSTQAAADQVPSTSKLDGPPPPSSSFGIIKRENTFRYPSTQGSQNLAIHELIAPHITSFNALFDGPDGSPGLLGLAIADLPSKVVFDSEKQAGDPRDSRNRLEFKITEVMVSNPLAMEKGQGALRDRTYPTEARERLVTYAGKISAKVTWSLNNGPPQNEYRDFGLLPIMVKSEKCNLKQMSPAKLVSHHEESEEMGGYFVVNGNEKIIRCLIVPRRNHVISLIRPSFVNRGNFYTTYATQIRCVRPDQTSQTNTLHYLSNGTITFRFSWRKSEFMVPVVMLLKALIGASDQEIFTNLAQNAFEDTFRTDRIELMLRNFQYYSLPTASACLDFLGSKFKVVLGCPDDWGSEQIGRYLLQRIVLVHLKDPISKYRLLLFMIQKLYALVAGECCADNPDSPQHQEILLPGHLLSSILKERFEEYLGGIQAQITSEVRKSTTGVTFSDNKFVTKAIGKVYSDIGRRVTFFLATGNLVSPTGLDLQQTSGFTIIAEKLNFYRYISHFRCVHRGAFFAELKTTTVRKLLPEAWGFLCPVHTPDGSPCGLLNHFAHKCKILTSAPSVKNIPSLLLSLGMSQPFDTTIHPSKFVSVQLDGEIIGWASSSLCSRMATALRTWKTEGLENIPLELEIGLVPRSKGGQYPGLFLFSTPARMMRPVQLLSNKKTDLVGSFEQVYMDIACSQEEIDPGFSTHVEISPTHVLSLLANLTPFSDFNQSPRNMYQCQMSKQTMGTPSGVIHHRTDNKLYRLQTGQTPIVRPALHDVYQMDHFPNGTNAVVAVISYTGYDMEDAMILNKSAHERGFAHGMVYKSMIVDLSPEGSRTSSEKHFGIGKSFGGSESSAFNRMCDKLDSDGVALVGSRVRSGDPLCAYVDRTTGKTSFEKYKGDEEAFVEEVRMIGGDVPSKECTKIHFKLRVPRSPVIGDKFSSRHGQKGVCSQKWPSIDMPFSESGIQPDVIINPHAFPSRMTIGMFVESLAGKAGAMHGIAQDATPFRFNEQDTPSAYFGEQLRAAGYNYHGNEPMYSGITGKEFEADIYIGLVYYQRLRHMVGDKWQVRTTGKVDKLTRQPVKGRKRGGGIRFGEMERDALLAHGTSFLLQDRLMNCSDYSTAWICKRCGLLSSLGYDTSDDLGLAPTSQHHQAHQAEPKFSSSVKMRGPRGEYCRQCEPDFPLKAAEHALPNTQNGQSLESLSGQLTNHMDVIAIPYVFRYLVAELMAMGIKIALQAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3KM07</accession>
    <name>E3KM07_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_11525</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178294">
        <property type="protein sequence ID" value="EFP85356.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329775.2">
        <property type="nucleotide sequence ID" value="XM_003329727.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KM07"/>
    <dbReference type="GeneID" id="10543899"/>
    <dbReference type="KEGG" id="pgr:PGTG_11525"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11525"/>
    <dbReference type="HOGENOM" id="CLU_304055_0_0_1"/>
    <dbReference type="InParanoid" id="E3KM07"/>
    <dbReference type="OrthoDB" id="2498778at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173985" evidence="3">
        <location>
            <begin position="24"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="130"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="497"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="146"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="176"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP85356.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="978" mass="110662" checksum="3C5B111946996BE4" modified="2012-04-18" version="2">MFIMKKLGVGLTAFLMSYVVGNATPSGAVQTTPSLACSNLLHSVFDAPDPCKSLTFRNSKAGFWDKRFLKDVKNSARSLSVWRKAILEFQLSEGPPSIKGKLVKRNFLSPIQVEKSKNVWRHREDATPKSILDRGIISPAGSTYDPEEKQTDPSHEPPNMRTFWIGSGSHSNNFEDSSTMSDASSQSDFDAKTQSDEEGPASTHFSPDHSMRTTHGKLVRSTDSNAASEGKSVFDWIPEPNGMQLLRGGKKTKVYQRKAPVVPSPIFDEMETKWDAQLRGPLLRLQHLVEIKLQPNRYTTVDLMKEEAIEIQSILNSLVQILEQPKLIKYLKIKDNVLAYHESYAEGCRLLQEVADVWINVRNNLDRLEVHVKLKRFEDTLEFLDAIRGDVLDMVSQRMALEMKNLLREVENYVDLDGPHLAHPPSPLDSDPQPLPVNQKYLPFNTHPGPEQVPSASQNQHLSSDINFTPPVIPQVMRSIEDIADSAKSFLNTLGNLKECRDAMKKDATNVERVEEQRHVLENELQNLRWKLSESGTAKIWSELLTREKFEAKKVELDTVIQDMFAANYLIRAFQTVYLGRKVTYSHNNKTYHDLAWVISEGLKAALKSASARQMDGLADILKSSTTTHIMSINDASLVNVNALITRFEHAPPDGMSWIKLQGKKLAEILEFHEVNLSHKRAVSNIKSQLKEVRDSLHSESSMPYGVLLDYQIKPWLSKLDVFDINLGTDSLASIHQSIHVPMKNQFEWEVEKLIEEIVLLKNTILGLTPGFLKPPKYTRTKLLTRRSVFPLQYNNNSNLALATHFRIIKEKLLWGTSKRFQTAQLLPLTAPDGMLQVSKDFGSAKDNEKFSKHRGVIDDLIGLVTSIELYKVAGVCPMSQHGASPELDSAKQLWIAYGSKKTAVLEMARNWRAFGLIFRDIIEVMMPSKSIEERQTQTYSDLFEAYQQAIHTKDCCCKLTSSKNTVIDRQIIDFEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>E3KMV4</accession>
    <name>E3KMV4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PGTG_11132</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the small GTPase superfamily. Rho family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EFP85803.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330222.2">
        <property type="nucleotide sequence ID" value="XM_003330174.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KMV4"/>
    <dbReference type="FunCoup" id="E3KMV4">
        <property type="interactions" value="777"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KMV4"/>
    <dbReference type="GeneID" id="10544378"/>
    <dbReference type="KEGG" id="pgr:PGTG_11132"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11132"/>
    <dbReference type="eggNOG" id="KOG1991">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004196_0_0_1"/>
    <dbReference type="InParanoid" id="E3KMV4"/>
    <dbReference type="OrthoDB" id="760868at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000244">
        <property type="entry name" value="Nonsense-mediated mRNA decay protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000983">
        <property type="entry name" value="Rho family GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1390">
        <property type="entry name" value="ROC domain from the Parkinson's disease-associated leucine-rich repeat kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997:SF18">
        <property type="entry name" value="D-IMPORTIN 7_RANBP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10997">
        <property type="entry name" value="IMPORTIN-7, 8, 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="14" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0488">Methylation</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-0636">Prenylation</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="17">
        <location>
            <begin position="148"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1058"/>
            <end position="1089"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1095"/>
            <end position="1114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="1070"/>
            <end position="1088"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EFP85803.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1201" mass="135198" checksum="F7235ABC784FEDC5" modified="2012-04-18" version="2">MSEIRRKLVIVGDGACGKTCLLIVFSKGTFPEVYVPTVFENYVADVEVDGKRVELALWDTAGQEDYDRLRPLSYPDSNVILICFAIDSPDSLDNVQEKWISEVLHFCQGLPIVLVGCKKDLRTDPKTIEELRRTSQKPVTPEESRHHAEAHLNQLQANPGFLSTLFSLVASADQPLPARQAAAIYLKNFTRSSYDHQSTRITAKPIDQSLQNNKISDQDRTFLKQHLLQAIASVPSSISTLLLPTLAVIISSDYPDRWPEVLPQTINLIGSNEFPLIQAGLLSLLEIMRLYRWSGRDKSQLRSNAITLSFPILLQLAQSLISHPPENYGLLPTPTSLDQANSNIGTLLYLILKIYKTSITAELPIFQQQQIIPWGQLFLAVIRKPLKNSPGFPSDPDDQDRWGWSKAKKWSYFILNRLYSRYGSPSQLPTNMLQYKPFADNFISSFAGPILRLYLEQVELFIQEHDWMSRKVICHTIIFFEECIRPKETWSVLKPQIPVLLSHFIFPLLCITEDEVCEFENQPEDYVRSQFAEFFEDICSNPSTISAGFLLALASGRKKTMFMSLLTFITDVCAKYPADHTPRDKDGALRSLAHLATVITETKSIRPNIEEFFSTYVFPEFRSEHAFIRARTCEVVRKFEIAGSEWSKQELLNTAYQGVTQCLSDPSLPVRVQAALTLPELCEHPQVHEGVAPHIGQIMKGLLALSNEVDLDSLTQATRSLVSKFSDELLPFAADMSQALHQSYMRLMSEIADARRRLGDEDDDSSEEKVLVAMNILKTLQQLVVGLEGNVNVLYQVEAASISLIKYTLKEEIVEIYDEALELLDSTQFALKTITNEQWDLFEIVYDVYKTSGADFVAEMFPSLDNYLTYGASYISSHPDTMNKMLDIYLSAMTSKTLSCSDRIIACKLADSMLLCLRGHADGAVPMFLEHTMRIIQRGITTIDPITTKALLMHALEVVLNAIYYNPGISMDVLIKNGWSSEFFGEWFSRLSSFKRTHDKKLSLLAISAILSISISEGVDNILAQSSGQLVLGALTLFESLPDAIRTRFELEKSYNLDSDDGSDLCSNGSDEDENGENDDEDVIDQPEEIYRGHVTSSRTKTHDSPSDNVTVPPSSLWSDEILWETPLDRLDAYCEFAAVMKNIETSGHPVLNIITNSLSTDQRDRLQKILKQANEGGEQVMKGHIQEAVQASRSWAQEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>E3KN52</accession>
    <name>E3KN52_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11017</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EFP85688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330107.1">
        <property type="nucleotide sequence ID" value="XM_003330059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KN52"/>
    <dbReference type="GeneID" id="10544254"/>
    <dbReference type="KEGG" id="pgr:PGTG_11017"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11017"/>
    <dbReference type="HOGENOM" id="CLU_2016369_0_0_1"/>
    <dbReference type="InParanoid" id="E3KN52"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="123" mass="13716" checksum="8118E5209D662C84" modified="2011-01-11" version="1">MRVLKRLAVVRRDSRGGMGQDAVAAGSGTEKMCGVKLNNNIMNSSEGVREKYRVKRPQINPQQAGLQESRRKGNKMGSKTTTEDPLIKIRLISIWVSIWLDTNGFSGGFHTNTWVETPLEISQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNR8</accession>
    <name>E3KNR8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_11699</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178297">
        <property type="protein sequence ID" value="EFP85943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330362.1">
        <property type="nucleotide sequence ID" value="XM_003330314.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNR8"/>
    <dbReference type="GeneID" id="10539942"/>
    <dbReference type="KEGG" id="pgr:PGTG_11699"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11699"/>
    <dbReference type="HOGENOM" id="CLU_586795_0_0_1"/>
    <dbReference type="InParanoid" id="E3KNR8"/>
    <dbReference type="OrthoDB" id="2497963at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172747" evidence="2">
        <location>
            <begin position="22"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP85943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="466" mass="53532" checksum="9C71DD8227EACE05" modified="2011-01-11" version="1">MKAFFNLPWLMLLQKINKSASMWPPAPVGSGEEEFLDLLSVHWQLGQENHIHDYLSKPLYFSEDPALQFKFPDEVTNPSINQQGSQTHPELTFEGGTSSTRALSPTRDLHYKGDYTVTGEQTSGEGLTFNGGPTISSSTISYHNDGHAPRGGSTLGHNSLTSNGEFTSNDKHLILPSKRKADRLVSEMDEDRSLWQRKRRAFRKSQLRVSSAQRAKSGISKESDSKSSRSGSGSSNDFARNNLQLRKSPDQNRMSDVVQAIPHRRESKPELCPRFRYRIIGESLLSLSDEQIDFKEYPVFSDGMEKLDAELMRWTRAKENQVIRNRHRTVERITNLKKNVTKISTFLIISLLSRLDGHNNALTNKSKLEEILTFMETFWEERKAGEKTLDAKNFRWISTLPDILNPDDRINSDTTYRGYALWFITAEEVTKYWMKRNMKLFQSSKIFKMNDGKYDEEKISQEKINE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNT2</accession>
    <name>E3KNT2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11713</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
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                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
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                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
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                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
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                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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                <person name="Martin F."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
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        <property type="entry name" value="LRR_dom_sf"/>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP85957.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="575" mass="64485" checksum="73A847B84A1BF715" modified="2011-01-11" version="1">MPATPGSASKQRTTKSGLDSFAREELMASLSLTTNNPEGVQPPPQATTSPGRTSKLSLSQLPYEIKQWIVYWLNLLEELEEPFDSDDSDVEEVDDDEFTDKPTSRKSPQKILTRSQADNHPPPPVLSSPPPPESALKSSATRLNSILALSLVDRTFYEICRPFIWQTIDLEDYDLSKLRELRTEFLPRHAECVRRIWWRVSLTELDTYEPEGWMSASESEEGSGRRTEWSEAGRSTELLGILQMCTKTTSLDVDLRPAELDEHQEFIVLPREPTAKFLAPIAQMSQLTSIALTAPANGLPFSEAFLVRLIRDMAGLQSFTCCSIDAQAPDFEHSQSITTCESPLALHLSKLGSLKEIDLDQADCFDLSWSTIEWAGSLENLALDDCCRASLNAIHAFSSKFSRTLFSLELSDVPFDEEVFEGGPPTRTIAELETGLYKFSLPELTTLSISNELPIGFLETFEECKKIKLIELAINPSISASDIERLVNPDSWPQLEKVIIATADAELSDGQIEALELFFLSKGVQLEIDSDSDDENDDDDDDDDDDDDDEQIGYAFEDEIEDMQNEWMEDDDDEF</sequence>
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    <name>E3KP34_PUCGT</name>
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            <fullName evidence="2">Uncharacterized protein</fullName>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12015"/>
    <dbReference type="HOGENOM" id="CLU_2628691_0_0_1"/>
    <dbReference type="InParanoid" id="E3KP34"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="22"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86059.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="87" mass="9532" checksum="353124EA8917DCF6" modified="2012-04-18" version="2">MPVQNDPNFVRPSNNYCKALPSSSATQTTARQTCKQPQQPSSALASTSTCVACKTKNAKVNEKGIRFPIGQQNARDIQGHWLHSGFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3KP75</accession>
    <name>E3KP75_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RAVE complex protein Rav1 C-terminal domain-containing protein</fullName>
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    <gene>
        <name evidence="3" type="ORF">PGTG_12056</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
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                <person name="White J."/>
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                <person name="Kodira C."/>
                <person name="Yandava C."/>
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                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178298">
        <property type="protein sequence ID" value="EFP86100.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003330471.2"/>
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    <dbReference type="STRING" id="418459.E3KP75"/>
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    <dbReference type="KEGG" id="pgr:PGTG_12056"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12056"/>
    <dbReference type="eggNOG" id="KOG1064">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_257114_0_0_1"/>
    <dbReference type="InParanoid" id="E3KP75"/>
    <dbReference type="OrthoDB" id="342131at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043291">
        <property type="term" value="C:RAVE complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="DmX-like/RAVE_component"/>
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        <property type="entry name" value="Rav1p_C"/>
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    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
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    <dbReference type="PANTHER" id="PTHR13950:SF9">
        <property type="entry name" value="RABCONNECTIN-3A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13950">
        <property type="entry name" value="RABCONNECTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12234">
        <property type="entry name" value="Rav1p_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RAVE complex protein Rav1 C-terminal" evidence="2">
        <location>
            <begin position="861"/>
            <end position="1331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1088"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1356"/>
            <end position="1393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1093"/>
            <end position="1102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1356"/>
            <end position="1367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP86100.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1402" mass="154643" checksum="D22CF4637C18064D" modified="2012-04-18" version="2">MSSSYHQPVNLSSVNSSRQALSISPSHSNQLIICYASGLLINFFDPESWRLLQSINLIDHLPHLPSYHKSVDVVYLTEDNRRLAVCCGPLTAIFERPNTSSTFPFSWKLHSTFTCHIARILSINLKQSYCLVTGEDGLSLYQLEEESSDSDEPAHWIKRWALRGQACDQSALEVLDDETCLFVAIVVGSPILRTYEFPASSSKASSNRPQYSPKTITLSLPIHSIRFSSRQSLSSNHTNQLPFLSVEVVAPVLGTALHYYNLIQRPLPLAAPGNPPQRLDGPYKPTVQYLHHGMCAISVPVNSPRILAALPLDNLAEQFISQTEQLLGHDSRLNVIVLSNGEVNLARSTKNTTMLSANPYLLPKDMLELFLRPGTMVHGLMSMHTSPGKPGDLSCSIVARPLTVTGEIFIAQISLGALLARPALPSSNPRLVSMKRLPYGYTPLFTRTSLVASEHTTFAGLLVTASTKDQDTRYVCLCHPAKGSSTRASRLLAYATHTDQTKAPLKCNLTGSVQAVGCFDSQGWYCKSDPVNVTAEHSHLGSTGLFVTDLNRGIHISVQPLSYQKISLKGFSEGELPKALVVECSSLKQSGKSKCSDGGSSTICFSSSNRLLVWNMPEGLRMSDHDSGLYTSSHEVSFPSSFLLSTLAQSSSSPSSTVLALDTNGQLVRALLPENLCDPGVDCSFKLKVTMCHQIGTQKTLGLDDVRSQVILLEPQYSRFLAMLCVMSETKLSRTYRVIVVDLRQQPYSPGIVGFDTFEVERGSDGAENEAQLKWSDYQSRAHSLPNECFLCVCCGTDLVRIYTRASDGWWAQTTTVHSELGCISQISWLAGSDHSTRVLYQTAGHTLLGPPISIPERLSLPPWHPILLKNQLLFGNLSLSFATVACLSLALEGCLQPLTSLCQRIVTFGLEDDEESIVQELDDLANDSVLPGNLSPTILSQAHIDAISSAVDGRRIPLLSAEEQALILRLARAFLQVQTARTHGIDTYGCQYIFSMIDHLEDHVGTRADWDLGFGPSQNCGILSAQLSSTRGLIGQETSLILSKYSHQLGSRLDWKAARSVGLFMWLDPVEEVHSYLEKVAQAEYVSGGSGNDRSSVQALSSDGPREQDPAACSIFYMALKKKRLLMGLWKVAYGHADRPLMTKFLENDFSEARWKTAAQKNAFALISRQRFRFAASFFLLADRLQDAVNVCVRQLADWQLALAIVRAYEGDRGPAMDKLLTETVLPLGFSTGNRWLVSWSFRILGLDELACRVLVASWDDPLIRNHWTPSVSERFSVRIGPLDLSLVMLFNRLKRAHQSPLKWTDERALVLLSVEELINAGCTMLAVALVKMWHIDRPDLPPKRLPIASDPLPQLLLDPDSSQDPSTDHQPSNPIENPTPMPKKVLSQPVVPEFELSNFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KR58</accession>
    <name>E3KR58_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178302">
        <property type="protein sequence ID" value="EFP86783.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331202.2">
        <property type="nucleotide sequence ID" value="XM_003331154.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KR58"/>
    <dbReference type="GeneID" id="10541370"/>
    <dbReference type="KEGG" id="pgr:PGTG_13165"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13165"/>
    <dbReference type="HOGENOM" id="CLU_1482701_0_0_1"/>
    <dbReference type="InParanoid" id="E3KR58"/>
    <dbReference type="OrthoDB" id="10346037at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86783.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="182" mass="19781" checksum="7BA43E6858ABB29E" modified="2012-04-18" version="2">MCRAGLTQPTKACLKEHTNVIARVGPRHASVLNMAVSDQSGLNLGGSRHAIRPTNHSTQPTIKMDPFKRSSTPDMLKRSSTPENIFQYNPHPTSPGPIVGNSGSSEQVTGLAGHSILAPDVPAVKRSPAKKKPNLHSPRPQLRLSLRIFNQGHGLGSFTTPASYFSVKETRKYRSQLPPPQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3KRS8</accession>
    <name>E3KRS8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Condensin complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_12744</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CND2 (condensin subunit 2) family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178303">
        <property type="protein sequence ID" value="EFP87003.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331422.2">
        <property type="nucleotide sequence ID" value="XM_003331374.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KRS8"/>
    <dbReference type="FunCoup" id="E3KRS8">
        <property type="interactions" value="578"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KRS8"/>
    <dbReference type="GeneID" id="10542078"/>
    <dbReference type="KEGG" id="pgr:PGTG_12744"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12744"/>
    <dbReference type="eggNOG" id="KOG2328">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010510_0_0_1"/>
    <dbReference type="InParanoid" id="E3KRS8"/>
    <dbReference type="OrthoDB" id="362021at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000796">
        <property type="term" value="C:condensin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007076">
        <property type="term" value="P:mitotic chromosome condensation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022816">
        <property type="entry name" value="Condensin_barren_su2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13108">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13108:SF9">
        <property type="entry name" value="CONDENSIN COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05786">
        <property type="entry name" value="Cnd2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017126">
        <property type="entry name" value="Condensin_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0226">DNA condensation</keyword>
    <keyword evidence="8" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="253"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="693"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="788"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="869"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="10"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="21"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="55"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="258"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="694"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="796"/>
            <end position="808"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP87003.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="911" mass="101134" checksum="E188356DAE1D6498" modified="2012-04-18" version="2">MAIKRPRSAHRTEGGDKANESIPMSPSSSSSTASDTGREPKRQRSQKAPRRDKLSPSSATSSSPSSSEDGGEPENSADWNQKEGISVQKKKNSTLAEHAKRRLSGKFGMVRKSMNFEQPPSGHSSPARVPGNGRVGALARARENHAKTRQSIGITQDNPLVISTEVMNNNYDEWMKMATDNKINVNNTWSFALIDYFHDMSLLRNPEAEGGINFQKASCTLDGCVKIWTSRVDSVATETGKLLSGLAEEANNISNQIDGEDDEDVGEESGLKAPKKTRTRATNSTSLVEFNEKIKVKELELEFTVDPLFKKTCADFDEGGSGGILMSHLSVDSSMTIIFDASGKLFSQNSDDDQATQNTAEDEHPTLLDVSKLKSAFLDSVEGFQARSICPCLSNFKFSTTDSIQSFEGLAALANIMEKPDEPPITHDTHDTGNDFFDSNESPNLGEDNFQIPDHEDVDDEGVDMGEAFNPSGIPNQRDLLMALVDDNSKQAEGGQSIFGQESTGMFDYFDKSMKKSWAGPEHWKLRRTVAFKSRDDENTTTVNRTKREKSTFSFDFLKPLENSRKSIFQAGSASLTLPGYKPPKPKSQRRMIKKIVGSKTAIGSKQMLEQHCLPDDMHFNASQLLRFDMKPKTTFNMRVKSSGSVAHHPGADIDENYWARTAAAAAQNNQDQANQEEENSNDAIPFATQFFQDEPDDQPDFEEEFDNPVGMTGNLSTDPDDTTLNDPAKTKEDEHDLIAATQNLNVRARPEFVNYAKKAKRVDVKKLKENIWRELEELTIEMEAEANTTDHQGESEDALEENVENPEGDGGSKRRKTKTFTKVINGLRTMYPQEKMEEISTSFCFICLLHLANEKGLRIQSISQSNQQQLPLDSAMNDDDGNIDDSAPLRPDDSKFVGRLEELKILKDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KS45</accession>
    <name>E3KS45_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13339</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EFP87120.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331539.2">
        <property type="nucleotide sequence ID" value="XM_003331491.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KS45"/>
    <dbReference type="STRING" id="418459.E3KS45"/>
    <dbReference type="GeneID" id="10542363"/>
    <dbReference type="KEGG" id="pgr:PGTG_13339"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13339"/>
    <dbReference type="HOGENOM" id="CLU_449874_0_0_1"/>
    <dbReference type="InParanoid" id="E3KS45"/>
    <dbReference type="OrthoDB" id="3265918at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="292"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="475"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="522"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="582"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="592"/>
            <end position="607"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87120.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="607" mass="67527" checksum="84F231B3A10FB818" modified="2012-04-18" version="2">MSLIRFTRSYSTTTTATTTTTTTPSTVIKHQFIHRQTKPKLTSSNDIQFLPPAKFTLSIISLSYNRPNVHALLSQSWPASRSIRHIDPNQLFILTAIPARFVNQNQPIPNHIHTLSSCFSKQDQERWKERRIEKQKSKAKPTHQLRLTFSLRIKELHKSAVIRKSIRKRWIAALKLIVQYGASSSRSTECTSAAAAADPKKNSAERRRTEEEEVRIDPAQAGFHRWLDPDHYYIVHPTLALNTVGLPQLIRAIREALLAIHPISNNPRHSRDTPSSTHPSRKPFLGTTHVASSPQSQSGSSSRTNNQHAPPTQSNPFIIKPQTHSLISESPSRRLPSKHPQFMCPQHSQSSPASLNKPRNTSTGSRSDHPPPFSPPRRRLPTSDPKFVSPQPTRSGPPASVHMKPSAPTSVIGTTPEDHSLKSSLEPPRKILPSRPIFTPPKQNLPTTLYKPRISSTGPGSHHHSSFEPPPRKIPSSQPKFIPPQQNHATTPTSLHMPSQISSTGSGSHHRSSFEPPPRKIPSSQPKFIPPQQNHVTTPTSLHMPSRVSSTGPGSHHRSSFEPPPRKIPSSQPKFFIPPPLSHSTATTTHQPSSLSPPRRLPIPLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>E3KSU1</accession>
    <name>E3KSU1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_13699</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178306">
        <property type="protein sequence ID" value="EFP87471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331890.1">
        <property type="nucleotide sequence ID" value="XM_003331842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KSU1"/>
    <dbReference type="GeneID" id="10538369"/>
    <dbReference type="KEGG" id="pgr:PGTG_13699"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13699"/>
    <dbReference type="HOGENOM" id="CLU_2278827_0_0_1"/>
    <dbReference type="InParanoid" id="E3KSU1"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP87471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="102" mass="11417" checksum="3D2CA4AE8F520143" modified="2011-01-11" version="1">MSCSVRPKVYWSRGKTWCGAKNNIIPVIKHEVKDKWSAFIGGLKLLDQAFLGSFAVGTMEGKPEGRSSARQIQPNVNLLTNGTHRSRLRGMEGEVSRSWKSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KT20</accession>
    <name>E3KT20_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13634</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178306">
        <property type="protein sequence ID" value="EFP87406.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331825.2">
        <property type="nucleotide sequence ID" value="XM_003331777.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KT20"/>
    <dbReference type="GeneID" id="10538135"/>
    <dbReference type="KEGG" id="pgr:PGTG_13634"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13634"/>
    <dbReference type="HOGENOM" id="CLU_041320_0_0_1"/>
    <dbReference type="InParanoid" id="E3KT20"/>
    <dbReference type="OrthoDB" id="2499600at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="39"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87406.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="377" mass="42680" checksum="13ABF42B4E288A04" modified="2012-04-18" version="2">MSSHNDGSPNTQNPSFIQRTKSSFESCFKLLLRITLFPFNFILARILGSFLAILFIALILYGFGSMFDANYSWLELTAGSRFLASLFPSLSGLYCSVFPSKFCQVQYSPTFDSKDKLGRIANTLSSTTTKASNVFDSIAHLSNPRNLEIYQTEIWELTFAIRYSSNLEEKDAMAEDLWDLGEIAGQLKDKFARVGQVIQLVGNGKKAYSIDTIKKNLEIAFGNFDWELKRLIDSIDDSIPLASRAASLGLQVSERIHKEHYRLQVFQDEQPLWRKLLDQTSKRGRRLHRDLQLTARSIQTARALPTGHEEVRSDLVSYQNHVSHFKAVISGWHLADHGLSAEDELDSMKSTINQLRGTISLAKRNSQDKESRVNVDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KTM8</accession>
    <name>E3KTM8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13411</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178308">
        <property type="protein sequence ID" value="EFP87625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332044.1">
        <property type="nucleotide sequence ID" value="XM_003331996.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KTM8"/>
    <dbReference type="GeneID" id="10538686"/>
    <dbReference type="KEGG" id="pgr:PGTG_13411"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13411"/>
    <dbReference type="HOGENOM" id="CLU_1939161_0_0_1"/>
    <dbReference type="InParanoid" id="E3KTM8"/>
    <dbReference type="OrthoDB" id="2508138at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172196" evidence="1">
        <location>
            <begin position="20"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="130" mass="13522" checksum="51CBF23C4234B90A" modified="2011-01-11" version="1">MKFAISLVLASTMLVSVQSDKPSLASFPCGADPKKTLGTCVLKTKDTIPIYYFAPATINNDMFTCTGVLVNKKNPTDPACCLPGTGPAKPPNALGGDATYGPTDPSTMGVVETEYNKNCTAPVAKDRGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3KTP5</accession>
    <name>E3KTP5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_13532</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178308">
        <property type="protein sequence ID" value="EFP87746.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332165.2">
        <property type="nucleotide sequence ID" value="XM_003332117.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KTP5"/>
    <dbReference type="STRING" id="418459.E3KTP5"/>
    <dbReference type="GeneID" id="10539144"/>
    <dbReference type="KEGG" id="pgr:PGTG_13532"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13532"/>
    <dbReference type="eggNOG" id="ENOG502S8KV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063740_0_0_1"/>
    <dbReference type="InParanoid" id="E3KTP5"/>
    <dbReference type="OrthoDB" id="70376at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070822">
        <property type="term" value="C:Sin3-type complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042826">
        <property type="term" value="F:histone deacetylase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013907">
        <property type="entry name" value="Sds3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08598">
        <property type="entry name" value="Sds3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01401">
        <property type="entry name" value="Sds3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="93"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="193"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="258"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="59"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="98"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="123"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="207"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="269"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="288"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP87746.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="298" mass="32713" checksum="E1CE15FF10741BD9" modified="2012-04-18" version="2">MSNPPTEINYLVRLHRESLKREQELVAVRVYHDHLISATRAAFDAEVRSIEEEFTNARTQAKEKLLESLEERRKKLKDEKELIDFNEEAVGHSHRTLTTRGLRNRSSNRTGFPRGSPALGGSSYPTNGSRLEGSSFNNPDHLSADPELGSSGTGLPPAAGFITADDPFSMATLNVDPHLRQSPIFQQLLQLGTHSGSRRGGPGAPPGGRRAPTGLPGAISLPSITPGTWNNFHKSQLGICSAKNDDTDADVEQIRHLAGLLSPKKRRMNNPSNPSNLNQSTTNPNSHTLHHHREKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>E3KTY4</accession>
    <name>E3KTY4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein phosphatase inhibitor 2 (IPP-2)</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13465</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
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                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
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            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
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                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
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                <person name="Crawford M."/>
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                <person name="Engels R."/>
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                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
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        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
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                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
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    </evidence>
    <sequence length="97" mass="10901" checksum="19A1FBC89857998C" modified="2012-04-18" version="2">MTEAIQWYKAKELQFKWNEQASAKGIPLSLVPNLREMWAARRKELNRDPVTRPWNESPPRSSSPEQSTPSKPKDSDSSSGGSNSKGKSASMVQIRID</sequence>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
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                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
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    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
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                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
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                <person name="Sakthikumar S."/>
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                <person name="Tuskan G.A."/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orc1-like AAA ATPase" evidence="1">
        <location>
            <begin position="30"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="182" mass="20692" checksum="FC477F54FD6AF554" modified="2011-01-11" version="1">MNRAHLISVFNGPGRLGRTYYSRPHEEERLSRRLKSAPQLTLILGPPSSGKTALARHVASQTRPDGTPEFHPLTVNLRAVDRFHDGSFLRAFVRNGIRAAEIDPFWERPFRGFDDLARVLYSKPPQDVPSSLMAAEVFNELAHRLQPLSYYTRESTRAPVLIIDEANHFRKMSDKVFVRVGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KV12</accession>
    <name>E3KV12_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_12559</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178311">
        <property type="protein sequence ID" value="EFP88112.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332531.2">
        <property type="nucleotide sequence ID" value="XM_003332483.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KV12"/>
    <dbReference type="STRING" id="418459.E3KV12"/>
    <dbReference type="GeneID" id="10535334"/>
    <dbReference type="KEGG" id="pgr:PGTG_12559"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12559"/>
    <dbReference type="HOGENOM" id="CLU_160865_0_0_1"/>
    <dbReference type="InParanoid" id="E3KV12"/>
    <dbReference type="OrthoDB" id="10322730at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP88112.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="111" mass="12274" checksum="D58A5F4F303E4E24" modified="2012-04-18" version="2">MSYKIGKFRRPLNPPIVGRLARYIKPPPRTFTEQQCVYLKNALAKHPDLAARFTTIIGNEDKIQIAEPGSDTVGSLADPATLGTQMAVPLLQPTNTRRETLPIKPPLSPWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KV30</accession>
    <name>E3KV30_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12577</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178311">
        <property type="protein sequence ID" value="EFP88130.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332549.2">
        <property type="nucleotide sequence ID" value="XM_003332501.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KV30"/>
    <dbReference type="GeneID" id="10535352"/>
    <dbReference type="KEGG" id="pgr:PGTG_12577"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12577"/>
    <dbReference type="HOGENOM" id="CLU_1982662_0_0_1"/>
    <dbReference type="InParanoid" id="E3KV30"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88130.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="126" mass="13885" checksum="5555875CC81259E4" modified="2012-04-18" version="2">MVFPELPIPRKVCTGTTTGSEKTVHYGWNGGPVHEENWKAALKGIPISYGANPITDIGTTLGIAKKHKRGHEKVINNGRPPDTTDKLVDGKKKKKFKLGRPPKATNFNKSLWSAYPSYQTNKDNTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVE5</accession>
    <name>E3KVE5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_14324</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178312">
        <property type="protein sequence ID" value="EFP88240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332659.1">
        <property type="nucleotide sequence ID" value="XM_003332611.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KVE5"/>
    <dbReference type="GeneID" id="10535550"/>
    <dbReference type="KEGG" id="pgr:PGTG_14324"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14324"/>
    <dbReference type="HOGENOM" id="CLU_107256_0_0_1"/>
    <dbReference type="InParanoid" id="E3KVE5"/>
    <dbReference type="OrthoDB" id="10297183at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP88240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="160" mass="18064" checksum="F984C4F55E5C3431" modified="2011-01-11" version="1">MDRNGIKIRKPSREQLILIKYLIKHVIAGRPYKFEEDGSFHDVPKSPWTVKYYCGKIEVFDEEDYLLYETSCCYRFPHSSAYCCSCDNLGEASLNTFAIICGPCTIVPSVSAIFLMVKEVGPRTIQRIRDAYSGTPAALWMRQQKATVAPAPETELGNLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVT2</accession>
    <name>E3KVT2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14382</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178313">
        <property type="protein sequence ID" value="EFP88298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332717.1">
        <property type="nucleotide sequence ID" value="XM_003332669.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KVT2"/>
    <dbReference type="GeneID" id="10535694"/>
    <dbReference type="KEGG" id="pgr:PGTG_14382"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14382"/>
    <dbReference type="HOGENOM" id="CLU_1396974_0_0_1"/>
    <dbReference type="InParanoid" id="E3KVT2"/>
    <dbReference type="OMA" id="PYYYELS"/>
    <dbReference type="OrthoDB" id="96345at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324:SF2">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="195" mass="21983" checksum="A53BB199B8F512E6" modified="2011-01-11" version="1">MIPRRPSPGSIETFFHGDRGPERLNSPVGPSLGNQPQVAPGIVWCQRFPSDWTLPSDKRHINLKPLPPMVRQPSWSGDGVDGGPSSITVLLNWLATNNNYVRWRQGESKRDLSSEILMEMRRNGIQHRDYHGILLQMTLLQRSHDQASKWESTTGSAVTAVGVDGSTVIVQRHLLRICRYWDEFGPIMRPAGDDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>E3KW71</accession>
    <name>E3KW71_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Poly(A) polymerase</fullName>
            <ecNumber evidence="13">2.7.7.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PGTG_14124</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Polymerase that creates the 3'-poly(A) tail of mRNA's.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>RNA(n) + ATP = RNA(n)-3'-adenine ribonucleotide + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11332"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17347"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="ChEBI" id="CHEBI:173115"/>
            <dbReference type="EC" id="2.7.7.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="15">Binds 2 magnesium ions. Also active with manganese.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the poly(A) polymerase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.19" evidence="13"/>
    <dbReference type="EMBL" id="DS178314">
        <property type="protein sequence ID" value="EFP88546.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332965.2">
        <property type="nucleotide sequence ID" value="XM_003332917.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KW71"/>
    <dbReference type="FunCoup" id="E3KW71">
        <property type="interactions" value="703"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KW71"/>
    <dbReference type="GeneID" id="10541012"/>
    <dbReference type="KEGG" id="pgr:PGTG_14124"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14124"/>
    <dbReference type="eggNOG" id="KOG2245">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011511_4_1_1"/>
    <dbReference type="InParanoid" id="E3KW71"/>
    <dbReference type="OrthoDB" id="412748at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990817">
        <property type="term" value="F:poly(A) RNA polymerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031123">
        <property type="term" value="P:RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05402">
        <property type="entry name" value="NT_PAP_TUTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.590:FF:000003">
        <property type="entry name" value="Poly(A) polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.460.10:FF:000002">
        <property type="entry name" value="Poly(A) polymerase alpha, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1410.10:FF:000001">
        <property type="entry name" value="Putative poly(A) polymerase gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.590">
        <property type="entry name" value="Poly(A) polymerase predicted RNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011068">
        <property type="entry name" value="NuclTrfase_I-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007012">
        <property type="entry name" value="PolA_pol_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048840">
        <property type="entry name" value="PolA_pol_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007010">
        <property type="entry name" value="PolA_pol_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014492">
        <property type="entry name" value="PolyA_polymerase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10682">
        <property type="entry name" value="POLY A POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10682:SF10">
        <property type="entry name" value="POLYNUCLEOTIDE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04928">
        <property type="entry name" value="PAP_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20750">
        <property type="entry name" value="PAP_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04926">
        <property type="entry name" value="PAP_RNA-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018425">
        <property type="entry name" value="PolyA_polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55003">
        <property type="entry name" value="PAP/Archaeal CCA-adding enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 13" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Poly(A) polymerase nucleotidyltransferase" evidence="19">
        <location>
            <begin position="17"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Poly(A) polymerase central" evidence="18">
        <location>
            <begin position="215"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="Poly(A) polymerase RNA-binding" evidence="17">
        <location>
            <begin position="364"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="528"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="571"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="588"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="651"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="96"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="109"/>
            <end position="111"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
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    <feature type="binding site" evidence="15">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="224"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="242"/>
            <end position="243"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR018425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR018425-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR018425-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF20750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="EFP88546.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="671" mass="74196" checksum="E532A9A0B39440CF" modified="2012-04-18" version="2">MNSMSPSNGSTPSRTYGVTGAISLQPATKPEQQLTVSLMEELKRRNIFESPEEARAREIVLGRLDVLVKQFVINVSISLGMSEAVARDSGGKIFTFGSYRLGVHGPGADIDTLCVVPRHITREHFFEVLPAMLNDRPEVTELATVPEAFVPIIKLKFSSIEVDLLFARLALPSIPDSLELIDDALLKNLDDRCVRSLGGSRVTDEILRLVPDVTVFRDSLRAIKLWAKSRAVYSNVMGFCGGVAWAMCVARVCQLYPNKPAGTIVNRFFAVLSQWNWPSPVLLKPIGPGPPGDQRRIWNPKIYPQDRGHRMPIITPAYPCMCSTHNITKSTAQIMMTEFKRGLAVTDEIATGTKPWAALFDKHDFFTRYRYYLQITASSPNAEIQLKWAGTVEARLRQLVMKVEEVETVDLAHPFIKGFERESYYLTSDEVRAIQVGDVPPDVKGRTLADIEGKEGSGAVYTSSFFIGLLVQPKPAGHTGPRKLDISYPVGEFTRMVKQWEGYAEDSMGIIVRYIKGAQLPDYVFDGEQRPSASVPSSKAFKRTKTGKAKQATSSIEIIPGQPTPGPIDLTCTTPTGQPSHSQHHEGLPQQQEPQLQERTTTMKKARPSSGLNNDSVDRNHTAITPPLSEMKLANQEAESSSSSNHDRELSNNPQHDPSPNLTNVFASVQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KXQ2</accession>
    <name>E3KXQ2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14955</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178319">
        <property type="protein sequence ID" value="EFP89114.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333533.2">
        <property type="nucleotide sequence ID" value="XM_003333485.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KXQ2"/>
    <dbReference type="GeneID" id="10546664"/>
    <dbReference type="KEGG" id="pgr:PGTG_14955"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14955"/>
    <dbReference type="HOGENOM" id="CLU_1267449_0_0_1"/>
    <dbReference type="InParanoid" id="E3KXQ2"/>
    <dbReference type="OrthoDB" id="406505at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174220" evidence="1">
        <location>
            <begin position="22"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89114.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="218" mass="24313" checksum="51F84D264C493AF8" modified="2012-04-18" version="2">MRFQLTTLFTVLVFLISCSQALESPASHADLEARDIDEEGYMNLSVGVRMKRDENNDSTLEKRSESPASTQLLLKRNPETGKMVWPGSFSEALTMAKRDVPEMLKSSLHRRTALARRAMKVVITWYTGHDLLYPSCFADSDNWAPTDDSMVAAVTIEWPGKPPCGSFVRIHHGSRNPNLLSSVLSTHALVAQVARHTLILRKPLSRSSTTRTSAWSQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>E3KXZ3</accession>
    <name>E3KXZ3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PITH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_15035</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PITHD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178320">
        <property type="protein sequence ID" value="EFP89194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333613.1">
        <property type="nucleotide sequence ID" value="XM_003333565.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KXZ3"/>
    <dbReference type="FunCoup" id="E3KXZ3">
        <property type="interactions" value="566"/>
    </dbReference>
    <dbReference type="GeneID" id="10546830"/>
    <dbReference type="KEGG" id="pgr:PGTG_15035"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15035"/>
    <dbReference type="eggNOG" id="KOG1730">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072377_2_1_1"/>
    <dbReference type="InParanoid" id="E3KXZ3"/>
    <dbReference type="OMA" id="SHEVTIC"/>
    <dbReference type="OrthoDB" id="2635at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.470:FF:000010">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.470">
        <property type="entry name" value="PITH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045099">
        <property type="entry name" value="PITH1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010400">
        <property type="entry name" value="PITH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037047">
        <property type="entry name" value="PITH_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12175">
        <property type="entry name" value="AD039 HT014 THIOREDOXIN FAMILY TRP26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12175:SF1">
        <property type="entry name" value="PITH DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06201">
        <property type="entry name" value="PITH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51532">
        <property type="entry name" value="PITH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PITH" evidence="3">
        <location>
            <begin position="22"/>
            <end position="195"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="11"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00025788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP89194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="214" mass="24169" checksum="99ADFDA0B4B8937C" modified="2011-01-11" version="1">MANPHQHHSCCSHEAHNQDDHHIKPGEGNQDFLFSKIALDQVTGYNVEPNRPAKSCIKPWDDRMDDQQFTESDMDQQLIIQIPFTGSVKLRTVIIRTLPGQFRPTHAHLFANEPTLDFDTLESRKPTQILDIPETTEVVEFPVRVAKFSSVTSLSIFFNSTTPGADKSQVYFLGFKGEFTNLSRKPVIAIYEAQANPTDHQKIKGLNDSVNHAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3L1H9</accession>
    <name>E3L1H9_PUCGT</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_16164</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178332">
        <property type="protein sequence ID" value="EFP90404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334823.1">
        <property type="nucleotide sequence ID" value="XM_003334775.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L1H9"/>
    <dbReference type="GeneID" id="10530298"/>
    <dbReference type="KEGG" id="pgr:PGTG_16164"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16164"/>
    <dbReference type="HOGENOM" id="CLU_2265058_0_0_1"/>
    <dbReference type="InParanoid" id="E3L1H9"/>
    <dbReference type="OrthoDB" id="10338559at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP90404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="103" mass="11302" checksum="F2B34DA13AF3DB11" modified="2011-01-11" version="1">MVPKRIWISYNKKLSSVPTQELEFVDDLAKAARNEFLPYLRHVATSDITLHVDEHAEALATVSALAEIVAPLSPCGISNTPLIVKGEKLHSIRVQALMPAMPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3L1M6</accession>
    <name>E3L1M6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_16038</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178333">
        <property type="protein sequence ID" value="EFP90451.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334870.2">
        <property type="nucleotide sequence ID" value="XM_003334822.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L1M6"/>
    <dbReference type="GeneID" id="10530345"/>
    <dbReference type="KEGG" id="pgr:PGTG_16038"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16038"/>
    <dbReference type="eggNOG" id="ENOG502SVCW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_676423_0_0_1"/>
    <dbReference type="InParanoid" id="E3L1M6"/>
    <dbReference type="OrthoDB" id="2501520at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173001" evidence="2">
        <location>
            <begin position="26"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="226"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP90451.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="404" mass="45298" checksum="5F3606F96C1FD276" modified="2012-04-18" version="2">MKNHNLYLILLTSITLLGSFNSTLADGIFNNIENAKLIQSSSIVPTDGNYVFKNVGTGQTIQHDRKNDVPNIYTSGDGGDSGSILPAAHHHRHHRARGGKHDDDDDDSLEVVSALELRSHRTRTKWISIRTTATTGDPKCISAQWGYHTEGGADHAGTLYECVVDPLNLEATLEKPKQWWLLMPVEAAKNLNSDQTLDLKNQILSQNKHMKMKDNPIISRIVEDQGKASGDQLPTPFWNPKTNRIENFSSPDKPKSKRHYDLSGERKRELENSLPTSHIANTTNAMPVAQALNVVHGDPNHNQDLIEKAEDFSHADASPLKMFDKRTTRHHIYSKTASQIHPKRKEADGSLGPFYVVSVDHLYDMETRVWTSAVKKTVGNQLSLVLKKLDPNDKTQQWYLDVQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>E3L209</accession>
    <name>E3L209_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_16610</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family.</text>
    </comment>
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    <dbReference type="EMBL" id="DS178334">
        <property type="protein sequence ID" value="EFP90584.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335003.2">
        <property type="nucleotide sequence ID" value="XM_003334955.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L209"/>
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        <property type="interactions" value="964"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L209"/>
    <dbReference type="GeneID" id="10530484"/>
    <dbReference type="KEGG" id="pgr:PGTG_16610"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16610"/>
    <dbReference type="eggNOG" id="KOG0389">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_16_0_1"/>
    <dbReference type="InParanoid" id="E3L209"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
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        <property type="project" value="EnsemblFungi"/>
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    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
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        <property type="project" value="GOC"/>
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    <dbReference type="GO" id="GO:0031934">
        <property type="term" value="C:mating-type region heterochromatin"/>
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        <property type="term" value="C:nucleus"/>
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        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:DNA binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:identical protein binding"/>
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        <property type="term" value="F:nucleosome array spacer activity"/>
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        <property type="term" value="P:double-strand break repair involved in meiotic recombination"/>
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        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="P:positive regulation of RNA splicing"/>
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        <property type="entry name" value="DEXHc_SMARCAD1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
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    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
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    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
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    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
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    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="356"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="760"/>
            <end position="915"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="904"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="120"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="156"/>
            <end position="169"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="907"/>
            <end position="931"/>
        </location>
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    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="984"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EFP90584.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="984" mass="110441" checksum="2F9C21425D69408F" modified="2012-04-18" version="2" fragment="single">AILVPSSSSPPPPLPSRSSSSSPSSSQGSSLVNDPLAFTVSPQVYLKSQALSKDKGKKKTRRIAPDSSEEEEQEQPQPTTSKDRRTRRRIDSDDSDEPVTILAPSKGNKKLANKRPRLASSSTSGSDSDPDCSIAIISAPQTRPTTKKLRRRIASDEEQEADENLDAEQDEWKRGSDEDDDDENHHQRVSEDDAIRFFNSCDVVDLPAVTACTLEQAKMIVGCRPFKDAEDMKSKLRKKKGVNAGILKNYQDMMKGYFEVDRVLSKCEKHGQDLSSIMNIWARGVQLASETTGGEGSKKKEEEGATDLVNHLTDAQGLTTEEERKAFADYIYTQPPTVPKKITLKSYQMLGINWMNLLYSKGLSCILADEMGLGKTAQVVCFLSQLKLAGQPGPHLVVVPSSTLENWKREFGNFSSNLVVREYYGSQMERMELRYELKAMPGLDVVITTYNIATGTVEDRKFLDRMRFDACVYDEGHQLKNSQSKRYKDLMRMHVRWRLLLTGTPLQNNLQELVSLLSFILPKIFGGQAQEDLRMIFKVPSEAQVNLLAQTRITRAKKMMSPFVLRRKKTQVLKELPKKIEKVIYCDLEPNQSEAYNQLIKKSKKYLMNSAGVEDDEELEEEEEVESFKKNGLLGATAGKKSPTKKTIANSTTNVLMELRKVSNHPLLFRRQFDGPMLRKIAQGCLNEVEFFDCSVELIIEDLEVMSDFEIDSFCAQYKSLRRFQLSRDEFFNSGKVKTLQLILASNNNLHADPSSSSKEIDKSKIVKQDSPSRFLIFSQFTQMLDILKVVLKLLDVKFLVLTGQTNVTERQSLVDQFTNDPSITVFLLSTRAGGLGLNLMAADTVILFDQDFNPHNDRQAEDRAYRLGQTRDVKVFKLISKGTIEEDILQLASTKIEIDNSISNPTPTVTSETATATPTTTHTTTTTATRTTDKDTATGVETNERSLPSADPVNHPPPLSENLVKKSLINRFRNRVLSSNVDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3L2H7</accession>
    <name>E3L2H7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_16836</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178337">
        <property type="protein sequence ID" value="EFP90810.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335229.2">
        <property type="nucleotide sequence ID" value="XM_003335181.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L2H7"/>
    <dbReference type="STRING" id="418459.E3L2H7"/>
    <dbReference type="GeneID" id="10530714"/>
    <dbReference type="KEGG" id="pgr:PGTG_16836"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16836"/>
    <dbReference type="HOGENOM" id="CLU_659111_0_0_1"/>
    <dbReference type="InParanoid" id="E3L2H7"/>
    <dbReference type="OrthoDB" id="10634002at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174116" evidence="2">
        <location>
            <begin position="20"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="82"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="193"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="207"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="313"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="341"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP90810.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="410" mass="41031" checksum="42FE2C889F329378" modified="2012-04-18" version="2">MMIKAAWIIVAALFIGKSAELAIPAKSDASSYGQYKPDVPQNPSPSWPGKPGSGDSDTENGTTKDADSKAGSGAGSPPTPTGPTSGLEPPSTHMNGTIADADSQPAPDGASPAASPTGPKGAFEPPSSQMNGTASDGDSQPAPISGGDNSPEQKNPNGSYQSPSSGMNGTIADAQGKPGSAPDSGPDDHSLGAPSQNGTLSSPVDKQPGGDQSGDDQPSGGQPAGGDQSSGDASGPGHPYVKRNPASTVSPYPAGKNSSDCDQPGPSPYQGSLNNGTQPALDDKDASSTTAPDEQADHSGDKKPLPDHRPGSEEGDEDSDDEDSDEDEDEDEDEDQDGEADGSKKKGQSKDASEGSADDGPSSVPPQTPANGEVQSPTSKKPADDHPDSYRSLASPADKHNKPAAPGAGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3L2R2</accession>
    <name>E3L2R2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17059</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178338">
        <property type="protein sequence ID" value="EFP90860.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335279.2">
        <property type="nucleotide sequence ID" value="XM_003335231.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L2R2"/>
    <dbReference type="GeneID" id="10530765"/>
    <dbReference type="KEGG" id="pgr:PGTG_17059"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17059"/>
    <dbReference type="HOGENOM" id="CLU_2237940_0_0_1"/>
    <dbReference type="InParanoid" id="E3L2R2"/>
    <dbReference type="OrthoDB" id="10278201at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90860.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="105" mass="11930" checksum="4EF4B72742112C12" modified="2012-04-18" version="2">MKDAPQNLRMNHIDISKRNRWANPNSIQIETLPVPTKVQRYHIAGKTPNRQEINDAKSPEEQHRNLVLERLGGLRNSITQRLKIHADSTQNEKAASQFAPNGSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3L323</accession>
    <name>E3L323_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_17220</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178339">
        <property type="protein sequence ID" value="EFP90948.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335367.2">
        <property type="nucleotide sequence ID" value="XM_003335319.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L323"/>
    <dbReference type="STRING" id="418459.E3L323"/>
    <dbReference type="GeneID" id="10528362"/>
    <dbReference type="KEGG" id="pgr:PGTG_17220"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17220"/>
    <dbReference type="eggNOG" id="KOG1177">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000022_59_7_1"/>
    <dbReference type="InParanoid" id="E3L323"/>
    <dbReference type="OrthoDB" id="10253115at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031956">
        <property type="term" value="F:medium-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025110">
        <property type="entry name" value="AMP-bd_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045851">
        <property type="entry name" value="AMP-bd_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201">
        <property type="entry name" value="ACYL-COA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43201:SF30">
        <property type="entry name" value="AMP-DEPENDENT SYNTHETASE_LIGASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13193">
        <property type="entry name" value="AMP-binding_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="2">
        <location>
            <begin position="84"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-binding enzyme C-terminal" evidence="3">
        <location>
            <begin position="518"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="554"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="555"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP90948.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="634" mass="69378" checksum="BDE1ACE194663CD3" modified="2012-04-18" version="2">MTAKMSSPGRIQRRLACSLTPLLISRPSASPQPRIPTILRHRPVSRWFGSDKHILPNYHDQPALISRAEPPSPTHQPGRKTGCLRLSYSQLDQSINAVAFGLHHLGIRKGDRVGINTPTHSIFPILQWATSRIGAILATINPAYAPSELANVLKKVDCKVLFSTPSFKSKDMIPVLSSLIQEIPSLTHLVLVDNESHQPGKRSKSEILEKFHSTKSSSSASSVIDLEQVIQQSSSSQQFPIPELQADDVINLQFTSGTTGLSKAVSLTHRNLLNNAHHIADRTTLTSKDSLCNVPPLFHCFGSVIGNLASFVKGATVVFPSETFDPDAVIRTVSEERCTVLNGVGTMFLAELNVLDRMKDVDMSCLRTGVVAGSPVSAELMNRIEDRLGIKELTILYGMTETSPGTFQTRPTDERSKRTATVGTIYPHTQAKVVDLATREVVKRGQRGELLVSGYSVQKGYWNDPLETAKAMITDQEGRVWMQSGDIASIDEDGYLKIVGRAKEVIIRGGENLYPVVIENCIIKLDGVVSVAVVGAKDEFYGEVVGAFVKRKPASSKSHSSPSGSERRTADQSVTEEDIRSIVKTNMAGANIPKFVWFLDSIGQVDFPMTGSGKIKKTELALWVQDLLSNHQNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3S1</accession>
    <name>E3L3S1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Adenosinetriphosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_16387</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF2/RAD54 helicase family. ISWI subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178343">
        <property type="protein sequence ID" value="EFP91196.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335615.2">
        <property type="nucleotide sequence ID" value="XM_003335567.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L3S1"/>
    <dbReference type="STRING" id="418459.E3L3S1"/>
    <dbReference type="GeneID" id="10528858"/>
    <dbReference type="KEGG" id="pgr:PGTG_16387"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16387"/>
    <dbReference type="eggNOG" id="KOG0385">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_0_2_1"/>
    <dbReference type="InParanoid" id="E3L3S1"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140750">
        <property type="term" value="F:nucleosome array spacer activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17997">
        <property type="entry name" value="DEXHc_SMARCA1_SMARCA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000082">
        <property type="entry name" value="ISWI chromatin remodeling complex ATPase ISW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000382">
        <property type="entry name" value="Transcription activator snf2l1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.30">
        <property type="entry name" value="ISWI, HAND domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044754">
        <property type="entry name" value="Isw1/2_DEXHc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015194">
        <property type="entry name" value="ISWI_HAND-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036306">
        <property type="entry name" value="ISWI_HAND-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015195">
        <property type="entry name" value="SLIDE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623">
        <property type="entry name" value="CHROMODOMAIN-HELICASE-DNA-BINDING PROTEIN 3-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45623:SF49">
        <property type="entry name" value="SWI_SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY A MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09110">
        <property type="entry name" value="HAND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09111">
        <property type="entry name" value="SLIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101224">
        <property type="entry name" value="HAND domain of the nucleosome remodeling ATPase ISWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="235"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="500"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="11">
        <location>
            <begin position="857"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="166"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="811"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1044"/>
            <end position="1125"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="914"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="27"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="67"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="167"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1098"/>
            <end position="1113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP91196.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1125" mass="128330" checksum="1C5EE902062299C6" modified="2012-04-18" version="2">MSDPASAINTQPNISSEQNGDPPAPAPVQSSSAQETSQTSAQPQPAPGSVDDEELSEQPGGASIDGAGDHSTTEAAGKPKTERQLRLDNEKAERAIARNDQRKIGDARDESLNFRRKEMDAGKVADSIKRFSYLLGQTELFRHFCDLKAQREPEFAKLLAESEKMCSKNKTKPKGQHRGRKTEKEEDEELLAADKQGDDSQSAADDEPFVFTESPSYVKGGTMRDYQVQGLNWMISLFHNGINGILADEMGLGKTLQTISFLGYLKHHRSLSGPHLVIVPKSTLDNWVREFDFWVPGFKLVSLKGSKDERGDICQQILAQDFDVIPWEYIVIDEAHRIKNVDSMLSQIVRLFQSRSRLLITGTPLQNNLQELWALLNFLLPDVFSSSEDFDAWFERKRNGAEDSSSDAENSVVKQLHKVLRPFLLRRVKSDVEKSLLPKKEINVYVGMTEMQRKWYKMILEKDIDALRKCCNHPYLFDGAESPPFTTDEHLVYNSGKMIILDKLLKAMKAKGSRVLIFSQMSRVLDILEDYCFFRQYEYCRIDGQTAHEDRIGAIDEYNKEGSSKFVFLLTTRAGGLGINLTTADIVVLFDSDWNPQADLQAMDRAHRIGQKKQVYVFRFVTENAVEEKVLERAAQKLRLDQLVIQQGRSTVQQKGQSKEDLVDMIQHGAEKIINSKEDMLVDDDIESIIQRGEARTAELNAKYSRLDFDDLANFKTENSTQKWEGEDYAGQAKKKLGLNWIEPAKRERKMNYSENISFREIMRGGPVAPKKKKGPKKINTSDWQFYPPRFIELQDRADLYYKKTHNIKAELPERDEGDNTSPEDLEKERQELQAEIDNAEPLNEEELAEKAEMQSLGFEAWTRRDFLNFIKGCEFHGRNSYDLIAKEIENKSVDEVQEYAEVFWQRHEEIEDHARFISKIEQAEAKRKKADRTEELLREKISSVKQPMQNLKIHYVNQTKGKSYSEEEDRFLLVQLHRHGLGKEEVYDLIKKDIIESPLFRFDWFIKSRTPQEIMRRCNTLVQLIVKELDPEDAEELMRKRVSKNKKDEEAGGGEGGQNSATAATTNGGGGTTNGKAKKRKTEDHLDHQNSPAVQDSAAANNLADSNASGNSTPNQRKRTRTKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3L539</accession>
    <name>E3L539_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_17520</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178350">
        <property type="protein sequence ID" value="EFP91664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336083.1">
        <property type="nucleotide sequence ID" value="XM_003336035.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L539"/>
    <dbReference type="STRING" id="418459.E3L539"/>
    <dbReference type="GeneID" id="10531603"/>
    <dbReference type="KEGG" id="pgr:PGTG_17520"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17520"/>
    <dbReference type="HOGENOM" id="CLU_1062227_0_0_1"/>
    <dbReference type="InParanoid" id="E3L539"/>
    <dbReference type="OMA" id="FEYNWAS"/>
    <dbReference type="OrthoDB" id="2503597at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174391" evidence="2">
        <location>
            <begin position="23"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP91664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="284" mass="30572" checksum="0792ADEEEEB52913" modified="2011-01-11" version="1">MFLRMIHLAITLVIAASSLSNAESLYPGSDTMFSPMSLQSIHGADSMQLLERYQIGGAGASVKPTVEVYSTKSTGSSFTKRDSTTEIPGITKPLDLTPSKCKSRVCYPGSYGGPKKTDCDAIVDAQLYHSVGSLTVKPGNYVLVYAGTCVVVFQHPMGKGDNNLTFDYNWSKLGLDIIEIQKKCDKPEALSIGGACRIKRYLQYNFDNILISLQRYVAPENPQTPIKPAPPSIPGNSEKPGNSGNSEKPGNSKPVNSKPGQPEKPGQPEKPKNPPQSQDAAPKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3L5B3</accession>
    <name>E3L5B3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PhoD-like phosphatase metallophosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_17475</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178351">
        <property type="protein sequence ID" value="EFP91738.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336157.2">
        <property type="nucleotide sequence ID" value="XM_003336109.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L5B3"/>
    <dbReference type="STRING" id="418459.E3L5B3"/>
    <dbReference type="GeneID" id="10531678"/>
    <dbReference type="KEGG" id="pgr:PGTG_17475"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17475"/>
    <dbReference type="eggNOG" id="ENOG502QPM1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013967_1_0_1"/>
    <dbReference type="InParanoid" id="E3L5B3"/>
    <dbReference type="OrthoDB" id="2100241at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07389">
        <property type="entry name" value="MPP_PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.70">
        <property type="entry name" value="PhoD-like phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018946">
        <property type="entry name" value="PhoD-like_MPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038607">
        <property type="entry name" value="PhoD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052900">
        <property type="entry name" value="Phospholipid_Metab_Enz"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43606:SF2">
        <property type="entry name" value="ALKALINE PHOSPHATASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G03860)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43606">
        <property type="entry name" value="PHOSPHATASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G08710)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09423">
        <property type="entry name" value="PhoD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PhoD-like phosphatase metallophosphatase" evidence="2">
        <location>
            <begin position="390"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="721"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="172"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="721"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="737"/>
            <end position="754"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP91738.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="770" mass="87316" checksum="4819285F42F11D17" modified="2012-04-18" version="2">MNPVILISLLITIQSVFIKLISSTWSPLTNLLSIQIRSLSYLFLRWIPAHHLPIAIPSLFLIYALAWSQHHSQLKQAYSEEESDDPQEEEEQDQREDDILNEDHLPVISSTPQNTPPSSISKSKKNKKSKNNPQQQSPNDLTTTTTATTYTETDHLIPKPGSNIKRKKTTQKQKNPNQQHPNHPVKSFLKSTIGLTTTNTSANKFTLIINTILTLFCLDATFRTSVLDGQEDLSFSRVAAIGETWAKVHARIPPIVYPSSSSSSSEKQETDFTTGGAQLVYRPMKPIGNWFIGPQLISTNQSDWMTVGKIDNLIPGNAYEYRLNLLDAVLEPHPYFDSVGHFQTAPDSSKLSDPRADGGSFTFAYSSCIKPGFPYNPFRNQLHNDGAEQLAEQARKLKLDFVLFLGDFIYIDSPIYLGNSIRHYWRKYRQSLSTTGWRKLMQFTRTIHIYDDHEIYNDWAGKGNDTNEIFEPANKAYRDYLGEGNFDGPGKGENYYWFRYGDAGFFVWDCRRYRSSNEQVDDHSKTMLGAEQKQVFLSWLHAVNSTVTFKFVVSSTPFMSLWLGPDGGVDTWAGFLTERSELLDSMQYVPNLIVLSGDRHEFAAASIRDTVIEFSTSPLNQFWLPIRTLSQENGLGKTGEDKLMKYIPNGIHKFSTLNVDCRDPMKPVVNFKLFIDDELSWELKYLGKPVKEEPKQLGQLLPTWTQFLNLIKRPIQWFSSDSHTRAPSLTPSSSSDEPSEEASSDSENASEEEGPLNHAESSARPIDDEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3L784</accession>
    <name>E3L784_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_18234</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178364">
        <property type="protein sequence ID" value="EFP92409.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336828.2">
        <property type="nucleotide sequence ID" value="XM_003336780.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L784"/>
    <dbReference type="GeneID" id="10538234"/>
    <dbReference type="KEGG" id="pgr:PGTG_18234"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18234"/>
    <dbReference type="HOGENOM" id="CLU_033090_3_0_1"/>
    <dbReference type="InParanoid" id="E3L784"/>
    <dbReference type="OrthoDB" id="10289122at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP92409.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="294" mass="33756" checksum="B9CCFF57A36E8F7C" modified="2012-04-18" version="2">MAEEQDPVLVGFSHLMNKYEPVFFGQWRDEENPPEEGDSSGLSDDQKLKTLQDLLDSMKHSLLPQLNDQLTTLSDLLFPSAFWNQPEITRKLVLETQPELERTIDQIKATIATLCPLPTLSTSTRTDDQDLKGLKYYRLQRLKSKFNEELLYHLNQLFTRAYFVFQQIRWVPDQVRPEQFDGWPCREEFAKHVRLGSLTIASMVLMIDCSELDLVQQQWAPELQNIEETMKEMGGLIARSSSGRPSPLPVEFEKLGSFSTRLPANIPPKTMVIRLAQLTLPLMMIQIQTEEGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8E6</accession>
    <name>E3L8E6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18885</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178374">
        <property type="protein sequence ID" value="EFP92821.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337240.2">
        <property type="nucleotide sequence ID" value="XM_003337192.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L8E6"/>
    <dbReference type="GeneID" id="10542534"/>
    <dbReference type="KEGG" id="pgr:PGTG_18885"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18885"/>
    <dbReference type="HOGENOM" id="CLU_039964_1_0_1"/>
    <dbReference type="InParanoid" id="E3L8E6"/>
    <dbReference type="OrthoDB" id="10283194at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92821.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="399" mass="43634" checksum="F5099E6D3432D85C" modified="2012-04-18" version="2">MFECDISFLMYCARKNKQKKTVWSGVRSKVKISISFNCHTIDLPRFKELVTLACGERHPNAPPLIDHGTNSSPPTMLWVAYMARNPKWLKTGSQSVATGPMFADWMQDIIKGGVKKGGLHLKMDNPSDNKSLAADNDLMAQTVCRHAAQMATLMSVLDKHPSTSADGPPGAPLSQTLPLGQTPFDLDDDAEDSCDESFDAQKIIEEEIFRKYSGNIGVDPSHSVYPHPTDVNWYVILTAGNHGKVKGVSLTSPPSCVKFYNRKTRKSTHVDPNEHPVTPHVPPLAASANRPDASPPMGQIVTLDLVASIVEICTETISKKQARSPESSSLPVGVAGSEGQLLDYVKFAGVANPEETMRVLERNEVDSYTMFAEGFFTPEQLQQLGLTVGTLAKLCRNKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8S6</accession>
    <name>E3L8S6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Ergosterol biosynthesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_18964</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERG28 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178377">
        <property type="protein sequence ID" value="EFP92951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337370.1">
        <property type="nucleotide sequence ID" value="XM_003337322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L8S6"/>
    <dbReference type="FunCoup" id="E3L8S6">
        <property type="interactions" value="164"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L8S6"/>
    <dbReference type="GeneID" id="10542843"/>
    <dbReference type="KEGG" id="pgr:PGTG_18964"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18964"/>
    <dbReference type="HOGENOM" id="CLU_114589_0_0_1"/>
    <dbReference type="InParanoid" id="E3L8S6"/>
    <dbReference type="OMA" id="ELMIFRT"/>
    <dbReference type="OrthoDB" id="6485510at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016126">
        <property type="term" value="P:sterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005352">
        <property type="entry name" value="Erg28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15451:SF19">
        <property type="entry name" value="ERGOSTEROL BIOSYNTHETIC PROTEIN 28 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15451">
        <property type="entry name" value="ERGOSTEROL BIOSYNTHETIC PROTEIN 28-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03694">
        <property type="entry name" value="Erg28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="8" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="11" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="23"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="109"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EFP92951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="14677" checksum="E5C4971A80CBF6B1" modified="2011-01-11" version="1">MARDIVIPILAGLPPHPGWLPKWMMLIAGVSILNGLQNYISLAGARKVYDRRPDRVVTSAPGLSPPVWSGSMRPYNISNPGLYHLALGTFAIALSHLVAEAVVYRTTGWGVVPPFIVASVSLGWMIKEYDHYLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>E3L9W8</accession>
    <name>E3L9W8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_19298</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
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    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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                <person name="Martin F."/>
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            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
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    </evidence>
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    </evidence>
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        <source>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
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                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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                <person name="Martin F."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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            <begin position="63"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93607.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="4">Hydrophobin</fullName>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
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                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
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    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003338122.2"/>
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    <dbReference type="KEGG" id="pgr:PGTG_19778"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19778"/>
    <dbReference type="HOGENOM" id="CLU_3260772_0_0_1"/>
    <dbReference type="InParanoid" id="E3LB26"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174339" description="Hydrophobin" evidence="1">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93751.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="42" mass="4425" checksum="3627D4E13FDA762B" modified="2012-04-18" version="2">MTSSPHHIIVLVVLLLVSSSLALPPGPTPPPDCCTKERPDCC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>E3LBH5</accession>
    <name>E3LBH5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Brix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_19928</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the BRX1 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="KEGG" id="pgr:PGTG_19928"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19928"/>
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    <dbReference type="PANTHER" id="PTHR13634">
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    </dbReference>
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            <end position="230"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="5">
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    </feature>
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            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP93900.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3LBJ5</accession>
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        <recommendedName>
            <fullName evidence="2">DM2 domain-containing protein</fullName>
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            <taxon>Puccinia</taxon>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19991"/>
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        <property type="entry name" value="SWIB_MDM2_domain"/>
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    <dbReference type="PANTHER" id="PTHR13844">
        <property type="entry name" value="SWI/SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY D"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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    </feature>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="1">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="688"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="745"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="766"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="815"/>
            <end position="837"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="847"/>
            <end position="872"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP93920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="881" mass="101077" checksum="8128FB9BAB169A96" modified="2011-01-11" version="1">MNNQQQQQQQQRQLPSSLTPQQQQALLQSQIIQQQQLQQQQLIQQQQQKRLQQANKPPPPQTRLTQQQQQQQQQQQQASFIQKQQQQQQALLLQQQQQNPAQHQLQLQQQQQQQQQQQLQQQQQLQQQQQQLQQQQQQHHQQQQQLQQQLLHKHHQFQSAPGHPQPNLNPPSNIKRPSPSYADALGPNIAFTRAKRFRPTDRNLPVFEPANPEAPRYSLDGLLPERQNNLHRMAQNYEKLQKIEREFDWLISRKRFEIEDSLRKPNGVLRTLRVKVWNTVSNQPWQQQQGSSSAAAAASSSEKKLDKTRQSSPGTDEDQPEKDNEQQDKENENEKKDDADHGDLPKQAAGPGSSAPINFNAGEGVPKWTLHIEGHLIDPNHQSSTDQTMADAESSEPQDQEPKIEETRRPFSTLMESMMIKIMRSHELYPEPNLVDWHRPSSTASAPSPLFSTITLTRNGTEDCPVQIAIHLNHFPRRFMINPLLGSFLDLKEASLDEIYESIWCYIKKNKLIDSGVDKRLIRKDSNLACLFPPNLDRMPFYQLSDQVRKFLSVPEPVIIEYEVKVDQEDQNRPEYFDIQFSIEDPAKLHLLTIQNQLEDINPASSLHQSATNNPANPTKEILAIDEQIMDSMAKIREVKIRRDFYQKFTLDPIGFIEEWIRSQSRDLEVLFGLDKGGEGTSVNRALSSKQPKSLSNHRFEAGEGVGDDDGLNEADHLKRYSKFYHQRWVDDAVKIYQSREFNHRVQQTQKSSSSSAPNLAHHHPLNNNNNNANNNNGGMMMMNPTGMVSNNPPPPMSHPQLNQHPHLIHHHHLQQQQQQQQLLNHPNSLSNNGHSNFNHAGPPSNLAISTNGPLINSNHLGNPGLINSNHNGAPGLRGNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3LC15</accession>
    <name>E3LC15_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_20077</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178439">
        <property type="protein sequence ID" value="EFP94090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338509.1">
        <property type="nucleotide sequence ID" value="XM_003338461.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LC15"/>
    <dbReference type="GeneID" id="10535789"/>
    <dbReference type="KEGG" id="pgr:PGTG_20077"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20077"/>
    <dbReference type="HOGENOM" id="CLU_1338091_0_0_1"/>
    <dbReference type="InParanoid" id="E3LC15"/>
    <dbReference type="OrthoDB" id="10460928at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP94090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="205" mass="23274" checksum="D88DD5CB96A99036" modified="2011-01-11" version="1">MFHSALGSRTSRRDPFTASIVMKLLSCYSTTLLPKLGKTSFHFTHHFAYLPATFKAIRRKQLGAIGPRSTSYLSIPDGGPLKFGFDVMKYLECNGYGLYTLLVHWKLLRNSKRKSSSNSKRILVFVFDTLSSIDQARGGNCFDPLVNRRNIVVDTELIRRFQLSFSPNFYLINRLSFERSSIPQYVGAFTSEEAEIAKAIINLNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3NXJ4</accession>
    <name>E3NXJ4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20241</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS989945">
        <property type="protein sequence ID" value="EFP94293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338712.1">
        <property type="nucleotide sequence ID" value="XM_003338664.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NXJ4"/>
    <dbReference type="GeneID" id="10527425"/>
    <dbReference type="KEGG" id="pgr:PGTG_20241"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20241"/>
    <dbReference type="HOGENOM" id="CLU_2134760_0_0_1"/>
    <dbReference type="InParanoid" id="E3NXJ4"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP94293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="135" mass="15645" checksum="58F30B67D380E973" modified="2011-01-11" version="1">MNMHSTKKRRSINRCDSGATNKGDRFRLFQVPEVSQSSATLLQHPPSPSNQPKHFHIRTQASFRQSLDDLSLDRHPGNLELVEPIRLFVQPTHLKPNRPAPPRHASHHLQLREKKELKSRSSSDLLSRKDNRDRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>H6QP82</accession>
    <name>H6QP82_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4219 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_20751</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EHS63167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890722.1">
        <property type="nucleotide sequence ID" value="XM_003890673.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QP82"/>
    <dbReference type="GeneID" id="13541405"/>
    <dbReference type="KEGG" id="pgr:PGTG_20751"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20751"/>
    <dbReference type="HOGENOM" id="CLU_2942854_0_0_1"/>
    <dbReference type="InParanoid" id="H6QP82"/>
    <dbReference type="OrthoDB" id="10378475at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="60" mass="7043" checksum="588E7F9BC48809B5" modified="2012-04-18" version="1">MEKINAMILKTAIKAIPLLTQDNYSMWNSQMMNFLELQKLKDTFFKEDMENITGNDELQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRL8</accession>
    <name>H6QRL8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7872 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21504</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EHS63288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889861.1">
        <property type="nucleotide sequence ID" value="XM_003889812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRL8"/>
    <dbReference type="GeneID" id="13540712"/>
    <dbReference type="KEGG" id="pgr:PGTG_21504"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21504"/>
    <dbReference type="HOGENOM" id="CLU_030195_2_1_1"/>
    <dbReference type="InParanoid" id="H6QRL8"/>
    <dbReference type="OrthoDB" id="2501761at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057194">
        <property type="entry name" value="DUF7872"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33339">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33339:SF1">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25278">
        <property type="entry name" value="DUF7872"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7872" evidence="1">
        <location>
            <begin position="2"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF25278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="219" mass="24414" checksum="2D4F7F7553B38806" modified="2012-04-18" version="1">MMAQAQRAVQSTISNITQNVLNSPINHLEGLAGLNRDGSFLNEVPSNFQNRLQRELELALKLKSLAKFWRVQNAFIVRGSDPCIYSGVNGAFDDADKLSYCGEDNIMMNIVRAEKNGTGYNPTIHHASLVESKYGYSSKFLTTLAWECQSKHGVFEYDSSSFHNSSATNQSELRDLAKSGDCYFNLPVCDLTRPDLKHLRSKTKKTLTQICREAGGLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRN9</accession>
    <name>H6QRN9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21634</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178284">
        <property type="protein sequence ID" value="EHS63333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889836.1">
        <property type="nucleotide sequence ID" value="XM_003889787.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRN9"/>
    <dbReference type="GeneID" id="13543023"/>
    <dbReference type="KEGG" id="pgr:PGTG_21634"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21634"/>
    <dbReference type="HOGENOM" id="CLU_298617_0_0_1"/>
    <dbReference type="InParanoid" id="H6QRN9"/>
    <dbReference type="OrthoDB" id="5149081at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052055">
        <property type="entry name" value="Hepadnavirus_pol/RT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050:SF7">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="985"/>
            <end position="1006"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1006" mass="113972" checksum="7805F0E39644A6AF" modified="2012-04-18" version="1">MRDVYKYETLGEWIILHKENTDKILRKDGFMVALRYDIRIRANTFAHRVVKNGVKSFLDISIFRQEVYDTAYAEARRYKELIFKEVNPYAIGGVRASWDRTRVPKSRPSQPTTTNREAPPTQPSQVLRTQTPSQPSRAKAEEVLGIKAETLTQTTQGAKGRAAADTKMEKAAERETEEVQCDMNIPRWKEALEKAGLSKTSADVLNGFVYGFHQGISEHDLGPDIPFYAPPNHSSASQAQPKIEELIQKEIAAGRMFGPYTADQVHRKFGFFRTNPLGAVVNGDGSLRVINDLSFPHRKKDIPSVNSKNDFDTTWDNFKAVENFLRKRTKTALLAIFDWEKAYQQIPKAPNQWPYLMLQNFNNQIIVDTRIAFGGVAGCGSFGRPADAWKQIMLKEFDLVTVFRWVDNNLFVKELTSAVEMDDIVARSNELGVKTNPTKISPFKVEQKYIGFIWNTSEKTVRLPEEKTQAQIRQINEILEEGKEFTFKQIKIMAGRLNHMSYMLPQLRCYLCSLYRMMSDWVYQHVPRPIPLDTRLIANPDPTEIGWVGDASTSLGIEVLIGRRWAQFKARPGWNRGSLPRRGIAWMETAAIRIGLLMMKKLDIRKGKMIIVWIDNTTSEEAVKNRKSRDVHVNKEWKLIQNILLEMQIDLSARRVTSKENRANALSRGNRSNHHLKNQVLSTLPNLYLLLSATLLSPLQSNLPFNQTKTFSLSERLPPPPFPPRIKRHIILDISKIKTFLANGNQLREPTSLDIHFLRGYKWNRLLSFNAAARKFMRYKIAVKETPFVLPISAGDLYGFCYWAGKNTDEYDTQDICSKTLAKYLYGLQAWHLYHSVEYPKESKAKVAVLLRASADTDAEAPPRPVKAPVTITQLVALTNHLVLEMVEGNPSQIPVIRWRIPANGCGWPLFRKKLAGIRVSQRIPAAGRGDGRLEGDPDTLPVDKSMRYLYPDGWKEALPAGPLEGGYPRIPARIPAAADGYPPAGADAQMAGKSSRISASARISG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSG9</accession>
    <name>H6QSG9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178294">
        <property type="protein sequence ID" value="EHS63706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889528.1">
        <property type="nucleotide sequence ID" value="XM_003889479.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSG9"/>
    <dbReference type="GeneID" id="13542796"/>
    <dbReference type="KEGG" id="pgr:PGTG_21785"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21785"/>
    <dbReference type="HOGENOM" id="CLU_2942860_0_0_1"/>
    <dbReference type="InParanoid" id="H6QSG9"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="60" mass="7471" checksum="2EEB78CABDECD589" modified="2012-04-18" version="1">MTTSSKKKKKKKKYSNISKKTPRRRRRRMQWFIDHQTINYSFSARLQVILKKYNPLQNHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSM0</accession>
    <name>H6QSM0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NUC153 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21834</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EHS63742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="GeneID" id="13541870"/>
    <dbReference type="KEGG" id="pgr:PGTG_21834"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21834"/>
    <dbReference type="eggNOG" id="KOG2318">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="InParanoid" id="H6QSM0"/>
    <dbReference type="OrthoDB" id="431825at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039754">
        <property type="entry name" value="Esf1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12202">
        <property type="entry name" value="ESF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12202:SF0">
        <property type="entry name" value="ESF1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="366"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="126"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="322"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="366" mass="40929" checksum="0F90C2E37FC937A5" modified="2012-04-18" version="1">MEFDEADLADECDADTNDYQPLEFSTDVLRHSKVKLTWDNEDPIRKRYTRLNTQKLTQEELDELDFGRFIAPGSSDEERGSDGDRAEEEDAEGGTRRRKAGVGMGDLRKKLGLVADEPAEIDDGELEISFKPGFVDRPLPSVAASDPPPPKSKNQRIKEKLKAKPESPAKPDDQHHDRTARGSKSDREKREADELALLMVDDLLPPVTAAHSDEPKSLGSDRHFDLSKILKLEKLSAKKAKLSTKTRRKLVAQLDGDPPDQLDRFQVDVDDPRFGPGLVRDPELAIDPSNPQFIKSKNMLELLDSTRAKRARPLTPPSPSMEDNKNNNDGDGERSMAGEENNRSVEQLLSHLKKPSLQTSKRARIG</sequence>
</entry>
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        <submittedName>
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        </submittedName>
    </protein>
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    </gene>
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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            <taxon>Pucciniaceae</taxon>
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    </organism>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EHS64571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="KEGG" id="pgr:PGTG_22363"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22363"/>
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    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="156" mass="16853" checksum="EE0FBB213C23D438" modified="2012-04-18" version="1">MSRQLDMESGSADSFAGADWPSLEPGSVSQGLQSPMNPVLLEKDYQANLADAFWRTSGSSASRGPKNKHVRIKPCLKGGANRKYWPHEGFMNSEESTGTNSKKSGKLVPDPSEAVTVTPSDLIHMSLCNVLGGKQEEGQGGSDFRVPNMKPLVFQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUC5</accession>
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    </dbReference>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22377"/>
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    <dbReference type="InParanoid" id="H6QUC5"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="134" mass="14974" checksum="A707C45875F2ACBF" modified="2012-04-18" version="1">MVFHKCPKFSSLLILDSERRIHVGTECNLEKMHLRARQHKVASSSFEDKTETTGLELCQTQKGASRTPDQAAAMGALLNEHKGAMLKESILKHHTEPILTRAQTLNQLNPFGEPHGLSHIGATRTLIIRSRQIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
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        <name evidence="1" type="ORF">PGTG_22446</name>
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    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178339">
        <property type="protein sequence ID" value="EHS64630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003888805.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUG7"/>
    <dbReference type="GeneID" id="13542443"/>
    <dbReference type="KEGG" id="pgr:PGTG_22446"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22446"/>
    <dbReference type="eggNOG" id="ENOG502RY49">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007520_1_0_1"/>
    <dbReference type="InParanoid" id="H6QUG7"/>
    <dbReference type="OrthoDB" id="27483at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33099">
        <property type="entry name" value="FE2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33099:SF7">
        <property type="entry name" value="MYND-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="915" mass="103436" checksum="8CC2A46F77BBDD92" modified="2012-04-18" version="1">MGGNLDSVLGLKSDFNKAFNAIKTSGTFARWETLPTTPPAAFLFDGIEEINLPLGEEQIRELIAKARLATSDRGNEATVLSFLEPEWQSYLVDLSKRVASKLGLDHPIRLDLYRMLILGTGAMFKPDTDTERIPGMFGTLVICLPSAHTGGEVVVKHNDECHTLRTSDATQSFACWYSDVTHEVLPVQSGYRCCLTYNLAIRPGHPRPAASVLDLKKVPLRKTLQRWLENVANSNTANEPSHLYRTLDHQYKEKTLCFNQLKNNDFARTDALRKLAHELPFEIFLASLEKEEHGTYQSKNYRYSAYDTDSGSDANHHAIDDVDEEETTCVVKSLRALDGTILVRNYDFDSQFCLVDDPFRGLRDPSEDYEYEGEGRGSVTHRYRRSAAVIVRRDKIGEFLAECTFRSPDDYDSCSSVDNRKSKSPGRDDCNSALRYLRQISSAPSAKTSMLDAMCRQYVSTSSKKLNMTDLLKISLKYSHYALFQTVGACHQGLLPVEFFDWAKEWLDKLRNVSLVEMYYQTWIPLLIQAYPSMADSMSIIRKMSNPSGDAAVPDRTWAHDVILRCVQSFPETNKRPTRSDAEWIVCAIFELEEAWASTHALFTSIFDRFPQIGATAFLLEVLFQLKTQGQAKHLAIPDTVELYKSLSSRVFNGHRKLSDIVSPAKSKPASEGLSSHFHYQRNTDTKKTSENDLVVTPRALAQFAYDLNQMSTDAANLLEPFIQEITEQCATFSAEDMDSLWMPFLHELIPVLASRSVSLNTPTYQRLVHQFVKHSEDKAVGPHPQAGVTFAVPEVACTCGDCGGLNSFLRNPSQRVGRFPMALARRQHLERELEKAQIVCTQETQNYGSPHSLIITKHRTLQDELKEWTERQNKLYGGLTRHIRPEHLRILLGAHEAARVQSLAVPRQEPTARR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUR5</accession>
    <name>H6QUR5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22465</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178351">
        <property type="protein sequence ID" value="EHS64823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888746.1">
        <property type="nucleotide sequence ID" value="XM_003888697.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUR5"/>
    <dbReference type="GeneID" id="13542041"/>
    <dbReference type="KEGG" id="pgr:PGTG_22465"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22465"/>
    <dbReference type="HOGENOM" id="CLU_103048_0_0_1"/>
    <dbReference type="InParanoid" id="H6QUR5"/>
    <dbReference type="OrthoDB" id="2503017at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="264" mass="29596" checksum="AE5E6EB9EBA13F66" modified="2012-04-18" version="1">MTTPALNKTNIKSSKTLNQNTSQNTESTMEKINSTILKTALEAIPKLTADNYTLWKNLNGTLSDTQDVQLRTIITSKIDSSIHPNVITHENAKKAREIWKSITSYFASTQPANRAQVFNELLDLSFNQSDIQGFITTVRSINSRLFEIGIDIPQDLITYILLKKLPPSLSNVSQQITHSDKSLTTDLVLDHLKLYSNDQSAIANRGSGSRSDPIALFSDASKKCKKTAHNVLSNHPEAKCWMLYPHLRPAHENKFNRSESTKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQ48</accession>
    <name>E3JQ48_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">SEC7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_00087</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307137.1">
        <property type="nucleotide sequence ID" value="XM_003307089.2"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JQ48"/>
    <dbReference type="GeneID" id="10546758"/>
    <dbReference type="KEGG" id="pgr:PGTG_00087"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00087"/>
    <dbReference type="eggNOG" id="KOG0928">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003907_0_0_1"/>
    <dbReference type="InParanoid" id="E3JQ48"/>
    <dbReference type="OMA" id="IIMWRSG"/>
    <dbReference type="OrthoDB" id="2503686at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032012">
        <property type="term" value="P:regulation of ARF protein signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1000.11">
        <property type="entry name" value="Arf Nucleotide-binding Site Opener,domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023394">
        <property type="entry name" value="Sec7_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000904">
        <property type="entry name" value="Sec7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035999">
        <property type="entry name" value="Sec7_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01369">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00222">
        <property type="entry name" value="Sec7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48425">
        <property type="entry name" value="Sec7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50190">
        <property type="entry name" value="SEC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SEC7" evidence="2">
        <location>
            <begin position="1113"/>
            <end position="1309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="389"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="582"/>
            <end position="802"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="807"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="849"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1522"/>
            <end position="1559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1680"/>
            <end position="1740"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="105"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="224"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="401"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="480"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="592"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="618"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="639"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="669"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="701"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="718"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="729"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="763"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="868"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="902"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1545"/>
            <end position="1559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1687"/>
            <end position="1697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP74131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1746" mass="191029" checksum="0729BEBED37CBF7F" modified="2011-01-11" version="1">MSNSPAESTTTASSRTRTPSPTNSFKSSTTTPTTPKQQQPPPIPIPHLLLDRSRLKPVPRQPKPAPIQTPPAFTIPTLRSKLRSTTNPSPHSNNPSQHKPASNRSVSNQSHNNNTSTTPQKTSTETQADQPAAEPQPAEPAAAEPQPEPPAESLPEPQAAQSQLEPTVESQPQPPAESTPEAQPQPPAEPQAEPPKAEDAQPDPPAETQPGLSPTVDSQQADISLPSPSEDSPDQPQAHKPSPTEPQPMPDHQQDHHQLTNSLPHSENNPDQIQPWQHQFNRILDSDHSSLERANSAAARLNSLAKLNGLKIPDLPDPPLHPSSDTDNSLPPFLMAKHIEETKKKAGLISPTLPIPQYPLPLPVSLGFSVGPNGRGVPRLSALQRKALEKMANQQDSQDLSTATTSLTRSHTTTGTESPKRLPNPIHSPSTDESHQRQQARSNLIRKLSSRGPNTRLKLSPPLVIPSPADLQADPPSIPNPQLSCSSQPIHPESAFDHPEPLPELITTPTLDTIDHQAQEIIPSVDHLALNTTTIDSVTVDLSRTQTPLLEENTMVLNLPPVVTDHSPAVALRYSLNSLDSQTDPKHAIRTAPTNSHNPTSGSETIIGESKPTVAAQSYPSSSPSHSHPVPESSWLDFSETNSKSFISDSPDLNTEPISETTLAPTPDPVASSPSVRSSIVSRLKARASLSSRIDRSTATGRSPSPGQPQSRQSSIDRGSEVPAERRRSTIHGNHRVSRPSNSSTVQRVRPTANETSQDRARQSYTTLASRVGSPSPSHHTDRPLRPTRAPPITQAHNVVGGRSAISRKSSTDLGPSQFPPTPHLVRTHMPVASAVDLARYNDQKLAPFPALLDSSPPGSPERARSPPRSNLKSSKSQGHSILSSASNFFRSPSRSQDENTSRAANQMVSSPPATVMASPPSYPVRMSRSDEEQEEVVEPARWQSMGDAAGKMNAAAEVSRSATIESNTSSTASMISSQRREQILARLSPFLRPSHPTASPDHRLNDPPRKLLWHQPVLQVVNATSVKDRYLFLFNDILVITKPIIEFEDCKGESVPKLPITLKHSFLTKSVVEIKNLRCVCTNQARRSSTLQDKHPIQSVNDEMSTPEHWFQQAFQEDPSRAIQSHLQSIHLEPTDSEIAKLLIATTDLDRRQLGQFLTSPDRTGILRSYLEQMNLAYMRIEDSLRSVLLSLRLPADPPAIERFLEEFGVVWTTSNPKVGLTPALVTRWTTTMIVLSEHLHDGLTDSMRYVAGFIGFPNDIIKTEVGFVESMVQAEEAFKAQGKPSSSIAAEKLETMLKKSFQSIRRDRLVQARASIEDTEKIKIEVEEEGDSRGSKLPSHIIYQIPSELVTIRIPEADPQFGIKLFGSGLSFEPSFLSFSQSSSASFRIKGKALGVRQVSMIKIGRRAAAYDGLPLDLTVSVERAFMKHTVQVGFLNHMMAKRKYLFSFLGPQPKTDFLDLVDDAQAAAKHHQPFPGQNNGPQAVAKTVALQVLIDSLILNEDLVPMNVNPYASTTVGLKVTHHNKSKKKELEEEKKPASSSTSNHQSNGNGKIRSNSLSETYIHTLGSSEKELHQAIIERRNQAQELHIRKRQQKLLKESLVSNLSGGRVNLNPYSCSNKDAPVGLADENQNIVALKRFIFVGSELIHLCQQNSLVPLVLGFLSMGMVDKNRLITSNPSAHAGQNHHHPHHHQANHFAKNGSRHLANPNAVPSGDLVSDGHSNPNGNGNGYHYQQHQHDYHVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3JR80</accession>
    <name>E3JR80_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00148</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307198.1">
        <property type="nucleotide sequence ID" value="XM_003307150.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JR80"/>
    <dbReference type="GeneID" id="10528122"/>
    <dbReference type="KEGG" id="pgr:PGTG_00148"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00148"/>
    <dbReference type="HOGENOM" id="CLU_691050_0_0_1"/>
    <dbReference type="InParanoid" id="E3JR80"/>
    <dbReference type="OMA" id="NGHRHIE"/>
    <dbReference type="OrthoDB" id="2501449at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP74192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="439" mass="49362" checksum="65433C842C3F2758" modified="2011-01-11" version="1">MQSWDDFFESPTPSPTESHNTPVRERPPHPGHHHPGNKPGHWGNAPEDRNPQILPLTNANMSHVGVSTGRGAGPARGRATPLSHFSPYQRSSPAQPIPQADSPEMDGTANRGEPNQSTDPIGRNRRLRGLQRQLGISNEVYAQAEHLFDIPLADRWPAMVLLLLHSSQPGSIFPEPTSVSPEPSDHSHLQLRDQYSPQLRAFIRNQLGQILLRADLESYGRAGARMSRFISKAPALIMKKFIDDQPESVRVNYLPSDFETSSAATAELMKLIRELLKDEKNAFADAILLGTQAVSAGHHRPMPTLDQLVIHVFREMDLTMSQLSDAQINSHPKMTHKVKVRIAYLRFLLNVHRRQLRNPAGFWELIDKDLEERRRKSPAYKAAFVHLILQKDRKLWNGSHMISDVPAEAQGLPTEPEIIAHLESIQQISGHYLNRPRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRF3</accession>
    <name>E3JRF3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_00704</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307754.1">
        <property type="nucleotide sequence ID" value="XM_003307706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JRF3"/>
    <dbReference type="GeneID" id="10546601"/>
    <dbReference type="KEGG" id="pgr:PGTG_00704"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00704"/>
    <dbReference type="HOGENOM" id="CLU_058279_1_0_1"/>
    <dbReference type="InParanoid" id="E3JRF3"/>
    <dbReference type="OMA" id="TEDRTDY"/>
    <dbReference type="OrthoDB" id="76215at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024752">
        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47072">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47072:SF4">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12776">
        <property type="entry name" value="Myb_DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like" evidence="2">
        <location>
            <begin position="84"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="9"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP74748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="433" mass="47294" checksum="C606F943A43A8220" modified="2011-01-11" version="1">MNFIDPAFLTGDILPPALPSSPSPPPAPATVPMPTSNKTSATKTKHKATPKPTVSAQPSKDAPKPKLSAPPKKEKVDSKPNHIWTNNQRSSLLELIASQNAAGHATDNGNLKKEGWSAVIKKLNLKHGLDLTSEQVKNQKNQLRKLFVDYQFLRNQSGFGWDEDISTVTADETVWDELFDAHPRREFSKLKDKPFPLYDLALSVFDGTAASGETAQSHLFPATTDAVKLPPPSKRKIVTLKSDDENKSDIEIDPTTSLKTATSNATTPKRVRESKNSVIRSEMEGINGAISAVSENSKELMGAFSKISSAISKSEPKEATQPINLDSTNSANNSSFVPSEPFLSTISISEKALDLCAEKFLGHVADKTYVEFISILENETKARTFLALSRNSNNRICQIWLEKEVGKVKKDLFQILPLGVDGVMDLTIACKGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRM5</accession>
    <name>E3JRM5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Fungal-type protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_00583</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74627.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307633.2">
        <property type="nucleotide sequence ID" value="XM_003307585.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JRM5"/>
    <dbReference type="GeneID" id="10546425"/>
    <dbReference type="KEGG" id="pgr:PGTG_00583"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00583"/>
    <dbReference type="HOGENOM" id="CLU_650759_0_0_1"/>
    <dbReference type="InParanoid" id="E3JRM5"/>
    <dbReference type="OrthoDB" id="2509909at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172079" description="Fungal-type protein kinase domain-containing protein" evidence="2">
        <location>
            <begin position="29"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP74627.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="379" mass="42670" checksum="934A8DA9050E495C" modified="2012-04-18" version="2">MFPQSALFTLRMICLPLACPLLIQVTMAAFPTDGDWGKFAEDLADSIGESPRIKRGQTDQLATFELNPMAAFFGQSPGSEWTQDVRLMTPAPHGSTGRDGHPTQPNKRRKSIQAPSNLSAVGPVQEHEEHHEVSPSHFDSGFWKHVQLCSPGQLDSAFKKILRPDRPTSHTSILSDKNFLTLELKSFLDEIQNKKHGPLADQHKKHYENTINQIYAKFDDSSLIPEMSKISNWPVTTTTSFNDESGYYVSLIIPVNDQGERYDPDILLKVFADLNDLLAHIHYLIRNHLNGINVLGHDDHEQDYQKLVLWLFGEVFNPKRSLPVLGETSLNGVHDGEFGAIQIWILKLLQDPTSLLNVSLKDIWLPSDQVVVMEAVQDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRP5</accession>
    <name>E3JRP5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Guanine nucleotide-binding protein alpha-2 subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGTG_00494</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.</text>
    </comment>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74538.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307544.2">
        <property type="nucleotide sequence ID" value="XM_003307496.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JRP5"/>
    <dbReference type="STRING" id="418459.E3JRP5"/>
    <dbReference type="GeneID" id="10546499"/>
    <dbReference type="KEGG" id="pgr:PGTG_00494"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00494"/>
    <dbReference type="HOGENOM" id="CLU_787870_0_0_1"/>
    <dbReference type="InParanoid" id="E3JRP5"/>
    <dbReference type="OrthoDB" id="1701769at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031683">
        <property type="term" value="F:G-protein beta/gamma-subunit complex binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052717">
        <property type="term" value="F:tRNA-specific adenosine-34 deaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002100">
        <property type="term" value="P:tRNA wobble adenosine to inosine editing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01285">
        <property type="entry name" value="nucleoside_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.400.10:FF:000009">
        <property type="entry name" value="Guanine nucleotide-binding protein G(O) subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.400.10">
        <property type="entry name" value="GI Alpha 1, domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016192">
        <property type="entry name" value="APOBEC/CMP_deaminase_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002125">
        <property type="entry name" value="CMP_dCMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001019">
        <property type="entry name" value="Gprotein_alpha_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011025">
        <property type="entry name" value="GproteinA_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079">
        <property type="entry name" value="CYTOSINE DEAMINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11079:SF149">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00383">
        <property type="entry name" value="dCMP_cyt_deam_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00503">
        <property type="entry name" value="G-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00275">
        <property type="entry name" value="G_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47895">
        <property type="entry name" value="Transducin (alpha subunit), insertion domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00903">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51747">
        <property type="entry name" value="CYT_DCMP_DEAMINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0807">Transducer</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CMP/dCMP-type deaminase" evidence="15">
        <location>
            <begin position="178"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="143"/>
            <end position="149"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00074402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601019-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EFP74538.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="352" mass="39432" checksum="4450C39724619CEF" modified="2012-04-18" version="2">MAQNVSSNRSSGKTTPLKQMEITHNRGGLTPSQREHYRQQVFGKICEGMRLCLELMNKEDIELENADLMKFVPMFNHCVNLEPRQPFPHEYLEPLSLLCGDGGIKKALDSGNNSVLLENMKYFFPELDRLFQPSYVPTDLDILHCEGKTCQGKATGKTETTMIDQSKLRMIDRSQQNLSDLLLMDQAIEMANEALVANEIPVGCVLVSKTTDKVLSKGRNRTNETKNACLHAEFDAIGGLHSVTPADKIDWNDVKLYVTVEPCLMCSSALRQIGINLVYFGCSNDRFGGCGGVVSIHNDPRLIHSQPLTALGGYRREDAIILLRKFYITENTNAPVPKKKTNRVLKMEIPDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3JST1</accession>
    <name>E3JST1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_01699</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75106.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319525.2">
        <property type="nucleotide sequence ID" value="XM_003319477.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JST1"/>
    <dbReference type="FunCoup" id="E3JST1">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JST1"/>
    <dbReference type="GeneID" id="10547548"/>
    <dbReference type="KEGG" id="pgr:PGTG_01699"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01699"/>
    <dbReference type="eggNOG" id="ENOG502QRAF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018582_1_0_1"/>
    <dbReference type="InParanoid" id="E3JST1"/>
    <dbReference type="OrthoDB" id="5598057at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13311">
        <property type="entry name" value="PH_Slm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046868">
        <property type="entry name" value="BAR_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046869">
        <property type="entry name" value="SLM1/RGC1-like_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043453">
        <property type="entry name" value="Slm1_PH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31941">
        <property type="entry name" value="CYTOSKELETAL SIGNALING PROTEIN SLM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31941:SF1">
        <property type="entry name" value="CYTOSKELETAL SIGNALING PROTEIN SLM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20400">
        <property type="entry name" value="BAR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20399">
        <property type="entry name" value="PH_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="295"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="441"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="517"/>
            <end position="536"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP75106.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="548" mass="60856" checksum="3B780D7997CCEB04" modified="2012-04-18" version="2">MSAILKKPTPTIKRGSGSAEHADQTEHKEVDILFDRFLEYKSITKGLISYYEDLAKLETHTASEMVNIGGHLPVPLREGNQFLPEGGWQSILYDTREQTRAMADYHTTFAHTITHSVLHTLNHVKNDIKIFITDLENEPRALASEVGQHRVDSTRLLSQLAQGIAHSKSNPHAMTAKDDPVLIHRQVEAQLKAQLNFENSLTHMIIEYQKKAFELEKRVNTDIQTAVKDFEAARLQAQEATNKHWQTIHNGITQLDPEIEWNEYAQRSGHLIPESITMRDAAKIAFPGQNDETTKPIKAGFLERKKRYTKNYKEGFYVLTPSGYLHEHKSSDPAKHGEPELSIFLPNCSISAPAPEGSKTFKWHVEGSKSTSGGNQMGSLRKMKKTLHIGRKDIAFSFKARTYAEMTQWWELLQHPAKSSFTQATVRSSMPPGAAVSAVSDIGLDKTSPQSEAPPTVTRRVAESSSDGESGGSSEEEDAAHSHDELSTAPTTPSGLHHDHPHMSPDAVSEGLPVYKGGDHTEAAMANLKEKPEIHPVQDVPQLTVSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3JTD6</accession>
    <name>E3JTD6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GCM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01803</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319629.1">
        <property type="nucleotide sequence ID" value="XM_003319581.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JTD6"/>
    <dbReference type="GeneID" id="10542583"/>
    <dbReference type="KEGG" id="pgr:PGTG_01803"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01803"/>
    <dbReference type="HOGENOM" id="CLU_005992_0_1_1"/>
    <dbReference type="InParanoid" id="E3JTD6"/>
    <dbReference type="OrthoDB" id="2506334at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036115">
        <property type="entry name" value="GCM_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90073">
        <property type="entry name" value="GCM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="582"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1008"/>
            <end position="1031"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1057"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="594"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1069"/>
            <end position="1096"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1110"/>
            <end position="1123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1123" mass="126305" checksum="84E4C985E5EDE98E" modified="2011-01-11" version="1">MSDGTSELKVTSQLDAIEAADFEPIKGKKIFFAPSGNEQTFIDHDCKLDDDGYPIYPNRQTVFVKTPDMVVRNFGFVGFTKTSNVEWRADRSWKMVRISCLGALICNEPGCQWVGSPPTAKDAIKEYLQTQPTRNPKCPGAAGKCPGKVAHQACNNTMTRFDENKLTNWAILRHHGIHHHPWPAPKKPDPLSKLKLKNEIMKNPTAGAFKLKLGKPTAPLNPFESVTKIHDSFVNSDRVRYHRRLILTDLGINPHKGGAGLGDKFLLDMFQWNHVGLLIISSSFKPGIEHFTFQTKWMAERLVSRDHNNEVYGGGLLSDVTYRLFETGYLLSTSMFCEETARWIPVQLSWIRGLSAEYYQAHFSTLFEQFDRPGFTVNDRDTLVRQVVDFSLAQREGFISAYMKVFRVTDRQQALSKLKGCHEHFRAQVTRVKRNRAVVPPEQEAQFQKLCLDLLILPEAGKPCHEQKIDELRRLFPKTKRWLDWWTMVDIEAILFPSKRAMITESANGKDNLPDTTNAQESMHRLYYMISEGKKCLLIGMAELFAFVKALEDDWHAVMRGVSIEYGAKNKSQQDLAISIGWQKPPKRNPANRKKVELNDGRAPDTTATLLPQPKNVGGRPKNSHNLIKDPFSTYISYSASQDSAKKNRCWLAAALESLYAMFNPLWLCGTNGLKSDIFTTLVKHFNSRSSWELSLSGHIRSFLSIGQNSLHAAANKISPGCFNPGVFCSADLFIDLLIDPHIRKQSKTIYPPRDLFTVTEVRTATCELQPEVDQVHPRGVREVTVLRIQKETFDSANIEPFSVQQLISVWTTDGISSVSGLCCKLCPSTKKQEEPHHIVERSVFTVENGGPPQHLYLLVQVSSIFDEADQQTFMAGLDFPFQLEFHGVSYTLFSRGFWNGVHYWSKMLKNIGGISGVWIHDDRENGGIARLISPDHTTIAGLAAHTSWVFYSRPWNRSEEKFVEDSIAKITQDNKGAPGTMPFAHLGQLLSLSPNRLLPATEYTSTAMSAGSTVSKGDPSASFTADPPEDLKREVSGLEDVKRKASGEFKIRLKVLKPSNPRQPTEQDNNHTKLKNADQKQPGKCEVKKVGESKSKTSVAKAKVIKPTRSATRASTRKALQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>E3JTQ8</accession>
    <name>E3JTQ8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Guanine nucleotide-binding protein subunit gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_00790</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">G proteins are composed of 3 units, alpha, beta and gamma.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G protein gamma family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319878.1">
        <property type="nucleotide sequence ID" value="XM_003319830.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JTQ8"/>
    <dbReference type="STRING" id="418459.E3JTQ8"/>
    <dbReference type="GeneID" id="10543089"/>
    <dbReference type="KEGG" id="pgr:PGTG_00790"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00790"/>
    <dbReference type="eggNOG" id="ENOG502S5Z5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_163540_1_0_1"/>
    <dbReference type="InParanoid" id="E3JTQ8"/>
    <dbReference type="OMA" id="CLTIINY"/>
    <dbReference type="OrthoDB" id="19232at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005834">
        <property type="term" value="C:heterotrimeric G-protein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031681">
        <property type="term" value="F:G-protein beta-subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007186">
        <property type="term" value="P:G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.260.10:FF:000003">
        <property type="entry name" value="G-protein complex gamma subunit Ste18/GpgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.260.10">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015898">
        <property type="entry name" value="G-protein_gamma-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036284">
        <property type="entry name" value="GGL_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041848">
        <property type="entry name" value="Ste18_fungal"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28189">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28189:SF1">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00631">
        <property type="entry name" value="G-gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01224">
        <property type="entry name" value="G_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48670">
        <property type="entry name" value="Transducin (heterotrimeric G protein), gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="7" id="KW-0564">Palmitate</keyword>
    <keyword evidence="10" id="KW-0636">Prenylation</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="G protein gamma" evidence="11">
        <location>
            <begin position="11"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP75459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="82" mass="9316" checksum="CD968E50184986DB" modified="2011-01-11" version="1">MLSRPQKQSMSELKLRRLTEHNQRLREDLERPRIRVSDASANLIRFCKTTRDYLVPSVWGPLGKSEDPYAPQAAGASCCSVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUE8</accession>
    <name>E3JUE8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01004</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320092.1">
        <property type="nucleotide sequence ID" value="XM_003320044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JUE8"/>
    <dbReference type="GeneID" id="10543553"/>
    <dbReference type="KEGG" id="pgr:PGTG_01004"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01004"/>
    <dbReference type="HOGENOM" id="CLU_097644_0_0_1"/>
    <dbReference type="InParanoid" id="E3JUE8"/>
    <dbReference type="OrthoDB" id="2495097at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="1">
        <location>
            <begin position="11"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="186" mass="20861" checksum="7947CC40251DC7F3" modified="2011-01-11" version="1">MTNNRQDIHPQGMNSGIMRGVGFRPGSDKGSTAGIYARRPNLSQSTLDDDDLRWDQLGDHNQFLCTRVQHFSKQCFRDNVDVIKSFGIPSWSNTEWNDFEQECNGIFSSAVVTHADFSNDAHKDKDSNPWTYGLFSYINRSTGFPVVPQSLVSGHGFRFPDFNCEIDFGTAPGIIEMIWAKVGGQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUP1</accession>
    <name>E3JUP1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">O-methyltransferase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_01097</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320185.1">
        <property type="nucleotide sequence ID" value="XM_003320137.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JUP1"/>
    <dbReference type="GeneID" id="10543758"/>
    <dbReference type="KEGG" id="pgr:PGTG_01097"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01097"/>
    <dbReference type="eggNOG" id="KOG3178">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005533_0_0_1"/>
    <dbReference type="InParanoid" id="E3JUP1"/>
    <dbReference type="OMA" id="LHITDEC"/>
    <dbReference type="OrthoDB" id="2410195at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712:SF2">
        <property type="entry name" value="O-METHYLTRANSFERASE CICE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_4G14580)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="4">
        <location>
            <begin position="219"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP75766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="458" mass="50926" checksum="75DCAF1FAF8803CD" modified="2011-01-11" version="1">MSRQSTSQQLVRLIAQAVTDLEEDTSTQLPGATTTELNLPVVAPEDDLEATPKRRSAIRNLKGAVHQLLATLLPVGIHVEEIYYSHLQTVAIDTVIKGRIADLIHSIDPESNKGGVHVHLLAQKAGMDPRKLTHILRFLAVRNIFCELNPLHWGNNRCSLPLRTDSPNSLLHLFVHMRDSVSMPALVAFPDVLLDNHGGGASSSDPNESAFQRYYKPGCDYFSWLAASEDNIKAKTFAKAMVETTRSNGTSGSAHYKAFDWKGLGPKGTLIDVGGGIGAAAYAVSAHLPEWKIVIQDRPEVIQAGKEEYRNVGSKANIEFEELDFFRDQPPHRIENADAYFLRHILHDWPSHLCVQILTKLRLASKPTTRLLICETSLEPGLIEPDSPLLSNGGMATSLSHHYNLIMLSLFNAEERSREDFQELFALSGWKLQSVTTLTTFLDRFIFEGLPDPAWKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUY0</accession>
    <name>E3JUY0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Charged multivesicular body protein 7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_01186</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320274.1">
        <property type="nucleotide sequence ID" value="XM_003320226.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JUY0"/>
    <dbReference type="FunCoup" id="E3JUY0">
        <property type="interactions" value="53"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JUY0"/>
    <dbReference type="GeneID" id="10532770"/>
    <dbReference type="KEGG" id="pgr:PGTG_01186"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01186"/>
    <dbReference type="eggNOG" id="KOG2911">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021165_1_0_1"/>
    <dbReference type="InParanoid" id="E3JUY0"/>
    <dbReference type="OMA" id="VEDRPMQ"/>
    <dbReference type="OrthoDB" id="10250120at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF96">
        <property type="entry name" value="BCDNA.GH08385"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172783" description="Charged multivesicular body protein 7" evidence="3">
        <location>
            <begin position="28"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="631"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="445"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="638"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="648"/>
            <end position="659"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP75855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="666" mass="74154" checksum="B246A34315E28D58" modified="2011-01-11" version="1">MISYNKLARLGLLWALLFLSHPESSLGMPKGNAAKRCHVCASRDGRQSSGMGAYRTQQPRERHETLACTTLQTQGPCNRQIEVQLHHCRLCGQYAWVPTGDCPSHFRPQPIYSDSDDIELIRQMASDSVDTSLRLMQDSNKIPSSLSEYLNSLNQFKSNDRIESLYSDYSSTRQSNPAGFHSNNATWQIILTDLLRLGLQTNSKEIQKGRGATSVEEQTEKEETPPDRLVLHLNDQLLESLRRPSIGKPYGLACPLWALSSLSAASSSAVLAPLNSFLSTGGWGPATGITTTMVRSISWASRAITRGTILDPDQRFDQLELDQKLNLVKIDWVNLLLLRQTADRVIERYQQHFVGLSPSIDCLYTQSQFIDQLTKDLFPSTQPNLKLSTTDLGILLKHLERDRKILISEKNVIKFVVPTSIESDRDGTKYSRVGPAPINEVDRGVLSVKQTIQQLNQQIQTISAQIEQRTQEAKKYIQKSQPEIAASHLKSKKALKTVLSRRIGVLESLNEVYLKIEQASTDAEIMNAYETSTKTLKSLLSVPALNVDRVAQTIENMEEVLADQKEIDEAINEVYDPSRAIDDQEIAAELASLVDIAPTPQKSQPQLLHPDPTPITTSTSVEELQAKLHALRVPESPQPTTSEQSTLHAEDHGEAKKIAENAPLTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>E3JV21</accession>
    <name>E3JV21_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Histone acetyltransferase type B catalytic subunit</fullName>
            <ecNumber evidence="3 9">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_01227</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalytic component of the histone acetylase B (HAT-B) complex. Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. Involved in DNA double-strand break repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the HAT-B complex composed of at least HAT1 and HAT2. The HAT-B complex binds to histone H4 tail.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the HAT1 family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3 9"/>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75896.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320315.2">
        <property type="nucleotide sequence ID" value="XM_003320267.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JV21"/>
    <dbReference type="FunCoup" id="E3JV21">
        <property type="interactions" value="742"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JV21"/>
    <dbReference type="GeneID" id="10532811"/>
    <dbReference type="KEGG" id="pgr:PGTG_01227"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01227"/>
    <dbReference type="eggNOG" id="KOG2696">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036024_2_1_1"/>
    <dbReference type="InParanoid" id="E3JV21"/>
    <dbReference type="OrthoDB" id="10253098at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010485">
        <property type="term" value="F:histone H4 acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.360.10">
        <property type="entry name" value="Histone acetyl transferase 1 (HAT1), N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019467">
        <property type="entry name" value="Hat1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037113">
        <property type="entry name" value="Hat1_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017380">
        <property type="entry name" value="Hist_AcTrfase_B-typ_cat-su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013523">
        <property type="entry name" value="Hist_AcTrfase_HAT1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12046">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE TYPE B CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21184">
        <property type="entry name" value="HAT1_C_fung"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10394">
        <property type="entry name" value="Hat1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038084">
        <property type="entry name" value="HAT-B_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Histone acetyl transferase HAT1 N-terminal" evidence="13">
        <location>
            <begin position="68"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with histone H4 N-terminus" evidence="11">
        <location>
            <begin position="112"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with histone H4 N-terminus" evidence="11">
        <location>
            <begin position="300"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="10">
        <location>
            <position position="351"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="323"/>
            <end position="329"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="363"/>
        </location>
        <ligand>
            <name>acetyl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with histone H4 N-terminus" evidence="12">
        <location>
            <position position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR038084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR038084-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR038084-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR038084-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF10394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EFP75896.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="483" mass="56266" checksum="095B4C53515FB765" modified="2012-04-18" version="2">MAVTKARSSPRKPRVVITVSCSVGVAFPPSIDTRQVTTTTHIGLCAKSTKESDRSKPQAMAGEDPERWACDGTGALSLRLARGKVAADQLAPSEQHLISSFHPKFVYPLFGDEETIYGYEDLDIQLAFNSSTLKNYLAINYAAALPEPDPVEKIIYKFIPPDYTKSEATFNADLESSGTKKFIPPGKKISSYRTLPSKGKTKSEHFLTSKRWERCTDDQMEDEQDVIYELWAADWKSPGFREYHRRMQILVLFYIEGGSYIEEDDDRWEFVVLFERRKVKTADGDSSPYSYHFCGYVSLYSFYHYPSSIRLRLSQFIVLPPYQSNGHGSMLYSQIFQYLLTRPEVAELTLEDPSESFEDLRDREDLKLLFDNKVFDEKPLSELVPVPSEWYEKTRTKWKLANRQFARLLEMALRWKFTTLEESDNTKLERLYRIAVKERLYRFNYDSLVDLSKEERREKLQDTFQNVMDDYDRLLRGERRQID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>E3JVB8</accession>
    <name>E3JVB8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_01324</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320412.1">
        <property type="nucleotide sequence ID" value="XM_003320364.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JVB8"/>
    <dbReference type="GeneID" id="10533235"/>
    <dbReference type="KEGG" id="pgr:PGTG_01324"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01324"/>
    <dbReference type="HOGENOM" id="CLU_2741268_0_0_1"/>
    <dbReference type="InParanoid" id="E3JVB8"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP75993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="71" mass="7899" checksum="32015CE01D7E5227" modified="2011-01-11" version="1">MMESDWARSEASFIAQIFSTRCSARRRQAYVPLVPEDFSASNLSLLVMEGSVDSEQLQYKVSSPPGLDQPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXL7</accession>
    <name>E3JXL7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ATP-dependent DNA helicase sgs1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02253</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321211.1">
        <property type="nucleotide sequence ID" value="XM_003321163.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXL7"/>
    <dbReference type="GeneID" id="10528894"/>
    <dbReference type="KEGG" id="pgr:PGTG_02253"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02253"/>
    <dbReference type="HOGENOM" id="CLU_960221_0_0_1"/>
    <dbReference type="InParanoid" id="E3JXL7"/>
    <dbReference type="OrthoDB" id="2506785at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="290" mass="32674" checksum="3FFE6D6882733F82" modified="2011-01-11" version="1">MDALAITTLCLRVSFSLDNRVGYIPLWDDDPAYIAEQDREKKAGMPQCRCSNCSPAKAENLIQSLSVANNENFDDILNDNFSLPNIYNIKYKYPQKAAMVKKRKFTEEDEAEINEFATLLMSDFHYHYNTNIRPGGSTQGSDIFDKDDCKAILAQLDTITNSLELKRLIGGDCFLGQSAWLFAWLSNYRLSNVNNCSTTKTPLISQKPKRPCGPAKSGSIRNPCSAKQLGVPRPPTKKELAAQESRKRSLARQEAKKKEDELNQRRREQVQQIMAASREAHCIAQGTSTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXU1</accession>
    <name>E3JXU1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_02327</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the exportin family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76866.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321285.2">
        <property type="nucleotide sequence ID" value="XM_003321237.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXU1"/>
    <dbReference type="FunCoup" id="E3JXU1">
        <property type="interactions" value="1088"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JXU1"/>
    <dbReference type="GeneID" id="10529051"/>
    <dbReference type="KEGG" id="pgr:PGTG_02327"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02327"/>
    <dbReference type="eggNOG" id="KOG2020">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011906_0_0_1"/>
    <dbReference type="InParanoid" id="E3JXU1"/>
    <dbReference type="OrthoDB" id="27218at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005049">
        <property type="term" value="F:nuclear export signal receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030620">
        <property type="term" value="F:U2 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030621">
        <property type="term" value="F:U4 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030623">
        <property type="term" value="F:U5 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017070">
        <property type="term" value="F:U6 snRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034501">
        <property type="term" value="P:protein localization to kinetochore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000055">
        <property type="term" value="P:ribosomal large subunit export from nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071528">
        <property type="term" value="P:tRNA re-export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000022">
        <property type="entry name" value="protein EXPORTIN 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041123">
        <property type="entry name" value="CRM1_repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041235">
        <property type="entry name" value="Exp1_repeat_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013598">
        <property type="entry name" value="Exportin-1/Importin-b-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045065">
        <property type="entry name" value="XPO1/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014877">
        <property type="entry name" value="XPO1_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040485">
        <property type="entry name" value="XPO1_repeat_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223">
        <property type="entry name" value="EXPORTIN 1/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11223:SF2">
        <property type="entry name" value="EXPORTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08767">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18777">
        <property type="entry name" value="CRM1_repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18784">
        <property type="entry name" value="CRM1_repeat_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18787">
        <property type="entry name" value="CRM1_repeat_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08389">
        <property type="entry name" value="Xpo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01102">
        <property type="entry name" value="CRM1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="7">
        <location>
            <begin position="32"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1067"/>
            <end position="1087"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP76866.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1087" mass="122877" checksum="8C2DE66E57D0DA4D" modified="2012-04-18" version="2">MEELLNPASDPILIDQVVQAAFNTQGPQQKQAMAILAQFQELPDSWQKVPMILENSVSQNSKYIALQIMDKLITTKWKALPETQRSGIKNFIVGYIVKMTRDEAQMVKDKAFVNKMNLILVQILKQEWPHNWPDFIPEIIASSRTSLSLCENNMVILKLLSEEIFDYSAEQMTQAKTKALKNQMCNEFADVFQLCNEILEKATKPSLITATLGTLLRFLNWIPLGYIFETSLIDHLINRYLEERQYRNITLRCLAEIGCIKDAGPEYDSKFVLLLTMVMSSVNRILPPNTDLKEMWDSQPQYEQEFVMALAVFLCSFLFKNSRLLEVPQHHDLLVNVHMYLVKISQVDDRELFKVCLEYWSKLVKELYDEQQSIPTDMGPLMGIGLNLVGGAGGVGSTSSHFGGGGVGGESQGRKLLYRDVCSNLRLVFIEKMVKPEEVLVVENDEGEVVREFLKETDTIVLYKAMREVLVYLTHLDVPDTEEIMLNKLARQVDGSEWSWNNCNTLCWAIGSISGAMNEESEKRFLVTVIKDLLGLTELKRGKDNKAIVASDIMYIVGQYPRFLKAHWKFLKTVVNKLFEFMHETHEGVQDMACDTFIKIAQKCRRHFITQQPGEQEPFVDEIVRNLDKITEDLSPQQIHTFYEAVGHMIAAQPNRPVQERLIAGLMRAPNAVWDRLMQQSAASVDVLANPDNIKLLSNVLKCNVSACMSIGSFFQPQIVRIYMDMLGLYKAVSGIISETVAAEGIIATKTPKVRGLRTIKKEILKLVDTHIRRAEDLNSLNDTLLPPLLDAVLGDYNRNIPAARDAEVLNIVSTIVIRLGPLLTGKVAAILELVFEPTLTMINQDFSEFPEHRVGFFKLLRAINASCFTALLALEPNRFKLMMDSVIWAIKHITRDIADTGLAILLELFTNVSTHTDPPTAAAFFQQYYLGILQDIFFVLTDADHKSGFKGQSQVLARMWGLVESASIAGPLFNPTEHDATMDNGRFLKEYTFNLLSTAFPHVQPLIIQQFITGCSETYSDAARFKSNLRDFLISLREYSGTSDGQHHDLFQEDKEALAAAKAQEQAAVPGMVKPSEIKDEDEEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXZ8</accession>
    <name>E3JXZ8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_02384</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76923.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321342.2">
        <property type="nucleotide sequence ID" value="XM_003321294.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXZ8"/>
    <dbReference type="GeneID" id="10529108"/>
    <dbReference type="KEGG" id="pgr:PGTG_02384"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02384"/>
    <dbReference type="HOGENOM" id="CLU_665865_0_0_1"/>
    <dbReference type="InParanoid" id="E3JXZ8"/>
    <dbReference type="OrthoDB" id="2508817at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP76923.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="425" mass="48767" checksum="B7BC8E0AE38BEBF4" modified="2012-04-18" version="2">MIDPEFELLASLTQACDQGLDAGQTAQVKLICISTVVGVPVNERVLDRLVQALKTLKSKGRKKNRALVRSITSLKNNDFKIEWEVRSSVCWILRMKQLPLLHHHVHELLKCLNPVSLHLDSNRRFREGLEILSEIDNCMDQISVSVASIWDSPSPADSQVNVETLTPFRRHRLVHSSQGILGIVSDVLGRFEGFISHFFVSDNPKLQTSSTNDSLVHLAVLDLGVVNFLIESLGQSDFVVFLREWSFRERLRNLDCPLTSLIILGNSSQIKRNPEKSLVRKQIEGLVTLYKLHRVLVLKICKFPHIKPGLIPETNFRELEALPVATSTLEYELEQIVQNLRLDHTRRPDFVKLTLAIDDLETAFKKIKDLLNQIFASPNHNFDREAYQYSQQWIQLWISQFNLAANNFFWISNAYITEKLTSIYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3JYC0</accession>
    <name>E3JYC0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cation-transporting P-type ATPase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_03001</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321464.1">
        <property type="nucleotide sequence ID" value="XM_003321416.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JYC0"/>
    <dbReference type="STRING" id="418459.E3JYC0"/>
    <dbReference type="GeneID" id="10529386"/>
    <dbReference type="KEGG" id="pgr:PGTG_03001"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03001"/>
    <dbReference type="eggNOG" id="KOG0209">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1390857_0_0_1"/>
    <dbReference type="InParanoid" id="E3JYC0"/>
    <dbReference type="OMA" id="WWGLASA"/>
    <dbReference type="OrthoDB" id="48943at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140358">
        <property type="term" value="F:P-type transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF7">
        <property type="entry name" value="ENDOPLASMIC RETICULUM TRANSMEMBRANE HELIX TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP77045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="196" mass="21732" checksum="37A999BBDB4AD909" modified="2011-01-11" version="1">MSQPGMERRSGLVCQMCFKEAKVTPPGGTKEQPQGSILNTYVVATVLGQFMCHLAALIYITGLCETTSLHTKEINLDAEFKPSLLNRAIYLLSTCQSFSTFAVNFQGQPFQKDIKENKPLLYGLLVAAAVAFCGANNFVPEANGWLQLVDMPTSFQMQLCIVMCMDFRGAMLVELIVKFLFSDVWPKGMVIKGIER</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3JYY5</accession>
    <name>E3JYY5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_03216</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <dbReference type="HOGENOM" id="CLU_050438_2_1_1"/>
    <dbReference type="InParanoid" id="E3JYY5"/>
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    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0048026">
        <property type="term" value="P:positive regulation of mRNA splicing, via spliceosome"/>
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        <property type="project" value="GO_Central"/>
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    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
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        <property type="entry name" value="RBD_domain_sf"/>
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        <property type="entry name" value="RBM"/>
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        <property type="entry name" value="RRM_dom"/>
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        <property type="entry name" value="TRANSFORMER-2 SEX-DETERMINING PROTEIN-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="RRM_1"/>
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        <property type="entry name" value="RRM"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="34"/>
            <end position="112"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="107"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="127"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP77260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="217" mass="25859" checksum="668BD55467C9B108" modified="2011-01-11" version="1">MERSRSPLGDRIGPANGSQTTDRGRSSDVVNPGNNLHVSGISLKADDRDLEELFSKYGTVTKSQLMRDPHTREIRGFGFVTMETSDQAELAISSLNGTEFLGKTLSVEKARRSRGRTPTPGQYRGPVKRDDPHRPYEPRGGYCYRYDPYDRRYDDRLRRDYDDTRYPVRREYDDRRYRYDDRVPSYDRYGYAAPMRDRYDDRYDRRRDERDYDRRRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3JYY7</accession>
    <name>E3JYY7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03218</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77262.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JYY7"/>
    <dbReference type="GeneID" id="10537806"/>
    <dbReference type="KEGG" id="pgr:PGTG_03218"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03218"/>
    <dbReference type="HOGENOM" id="CLU_440140_0_0_1"/>
    <dbReference type="InParanoid" id="E3JYY7"/>
    <dbReference type="OrthoDB" id="7489993at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="650"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
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            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
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            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="668"/>
            <end position="678"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77262.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="709" mass="76672" checksum="C07F9B6A3AA010FD" modified="2012-04-18" version="2">MLLPHATNSFGLDCPSSLGLDSDFTALTSSYTNTDFELNGSQYNPLEYTEDGLNRYTNDSYLHGQLVTNTQLPANNQIRLSPKAQLVTNGQLASNTQILVNGPLVTDGQLIVNGQRAADGQLFASSHHVANGQLVANGQLVANGQLVANGQLVANGQLVADGQLIVNGQLIADGQLDATGQFDANSQVINNGAIVKNPTPVPSSKPKRQRKKPVKKGLVLPNPPAPIPSPPAPLPSPPAPPISPETEAPNDTTLKEPALHPKKPKPPKHSQWSPQLVEQAKTKALDELIQLVAEDSTYIRLSADDTVELNAAYMEYQRQLYIIAYKRKLAIEPCLHYVGEGSNPRGPSNYNNFARYDPEASKIFNDKSMPPNNRARELGRLWNLLDLETQEKWKDPAFLEEITRGAEKSVDDSTNRKRKGTASFDSDKWANKLVADLRQLSRSSGLEGFLVLGSRGKDKADMFVGGTHLGEMFLDMYATKDDPCQLFSDLIKGHRAMKTISGTEPTLAIPEKKKKPREIITKHDKGSIQSNTEFIRGKLNKAIDLAVPGGYGRGWPGTNTQHTLTKLGVSLQVRENNLGVTPLHFCKRPGDMKDKQGQLVVAALEEDLVELVTISASNGRSSVGCAIADSSEHTNLTRVSVGFCTSPGALKTNATASTKPSKKRKRTGSNTHISNLAQDSILGGDPTISVNDASDQEDGEEVASGVEAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZA0</accession>
    <name>E3JZA0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_03331</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003321746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZA0"/>
    <dbReference type="GeneID" id="10538085"/>
    <dbReference type="KEGG" id="pgr:PGTG_03331"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03331"/>
    <dbReference type="HOGENOM" id="CLU_1489703_0_0_1"/>
    <dbReference type="InParanoid" id="E3JZA0"/>
    <dbReference type="OrthoDB" id="10268312at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP77375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="181" mass="20352" checksum="A25F91408553D058" modified="2011-01-11" version="1">MGNFCHRICEDSTWPTCPQIINPIRKLCNTITFINVCGDYATSSKSPNMVVLVLMRGAHVGKNWRPTYQIMLPTFSFYVFSCNLCSRQRRWPCDPGVPAAAQSSADPYRYIHPSGLLRAKLASRKYVWFTSAFGQFPLKPMSTEAAEMVRCSGRCPSRPRFTITARPTDSHLIAPRGARGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZQ5</accession>
    <name>E3JZQ5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03486</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321949.1">
        <property type="nucleotide sequence ID" value="XM_003321901.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZQ5"/>
    <dbReference type="GeneID" id="10538569"/>
    <dbReference type="KEGG" id="pgr:PGTG_03486"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03486"/>
    <dbReference type="HOGENOM" id="CLU_2237939_0_0_1"/>
    <dbReference type="InParanoid" id="E3JZQ5"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="105" mass="11982" checksum="C72012A12FF924AA" modified="2011-01-11" version="1">MGSFAKSVAHISRGFTERQKQKAGDWRQQAVLVRVRTNSIGVIIYLYQAIKVATRHAAGRKRWTALRVRNDHPTYERRGNGLGERGIRGMRSQDVQGKDKYDIKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>E3K0G1</accession>
    <name>E3K0G1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA (cytosine-5-)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_03742</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.37" evidence="1"/>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77786.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322205.2">
        <property type="nucleotide sequence ID" value="XM_003322157.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K0G1"/>
    <dbReference type="STRING" id="418459.E3K0G1"/>
    <dbReference type="GeneID" id="10540608"/>
    <dbReference type="KEGG" id="pgr:PGTG_03742"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03742"/>
    <dbReference type="eggNOG" id="ENOG502QPKK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002259_0_0_1"/>
    <dbReference type="InParanoid" id="E3K0G1"/>
    <dbReference type="OrthoDB" id="5376140at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003886">
        <property type="term" value="F:DNA (cytosine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006346">
        <property type="term" value="P:DNA methylation-dependent constitutive heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044027">
        <property type="term" value="P:negative regulation of gene expression via chromosomal CpG island methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.490">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.120.10">
        <property type="entry name" value="DNA Methylase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043151">
        <property type="entry name" value="BAH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050390">
        <property type="entry name" value="C5-Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00675">
        <property type="entry name" value="dcm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629">
        <property type="entry name" value="CYTOSINE-SPECIFIC METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629:SF52">
        <property type="entry name" value="DNA (CYTOSINE-5)-METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51679">
        <property type="entry name" value="SAM_MT_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1091"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1103"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="627"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR037404-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP77786.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1210" mass="138045" checksum="EFEBF03A1F860DB7" modified="2012-04-18" version="2">MADPDFVPSTSEPEDEDQQPDLSELNHRRRRNLEAEEPVLVLPLIAQKFKHYFDCLKTISNGTLDAIHHIPCLKTPRSSTRFQDCVNLDQIHWTSSRPWKISQNGEDFYQSVKIQNQTINIGSVIICERKTGREFSYRKARLTTVDDNYGSDTDDEREDKFQDIKPPKPNRRRFLIRALFERNQLKYFHGTWLEEAQHSFVGASGEPLELLLVDQCYEIELRFITGIEKVVWIAANKTEPPPIDSYYCRFHWKPNEGSFLDLPCVRKPNPPNPIKHPKSLCSTCVSVPREIEWLTDATNGHILLGKTDQYELLGFTLRGQETYRLNDFVRVADTSPSKPQVIGQIVEICGVRANWNADKTINLDDTPFRPPAKVYSRIASLKHKPVITIRKYQRKPWFQTEKDSRKFADDHRLWMTNQEQLVDVKSQLEGKCEIVHLFKDKTRQFVTVGSRREVSEESIINRDNCFYSILPTRKQAKYFCSVPIIDDGWEKAIISQEPEWVKIEHESSKGNQYADLVKNYGCGRIRHLELFGGIGSMSVALIELGLASQDETMFIDFSIPACQTLSTNFPRSTIICADVNEVLALMINGKTESGQDFLVDQRTGKVIRVNELPRPGDFDLITAGFPCGSHSTLNVLRKANDSKNALCATALSFIAYLKPDYLFFENVWCAGLLKTSFINPGNDSVLNKAFLRIINGALISLGYQVQFGVLQAAQFGSPQARRRIIFAGTRHGLTAIKLPEPTHHYPDEGLAILLPTNDEKSDHNGHRLVRADYRKCSSGALKAITVHDAISDLPEFEYLNPDRIMAEYCSRRPHRIRQNNDNQELITGRSELVPQLNRLVSFSSTLNHSTISLIGFDEFEYLTKPMNRYQSWLRKPLEWKPLVEAFIPKYPRVENSDSRNVQQRRTRYNDEDGDHYALGSDCRIRNWHVTPRFSAKVTERICNIPLKPNADHRHLPKPLRLKNLNTCRNPHDFDGNYGRLGFQGQFNTIITKMHPHNQNKCGAVLHPNQYRTLSLLEAQRAQGFPDRFKLEPAATKGKMDETVNQQLFKLIGNSIPIPISLSLSKSLLKAREQEYLKTKVKEVKKVKRESSSFVLPNHLSQSHSHDQESHQSTKLGADKGKQKIVDDDEIVEISHHQNHHLSGNHTNLGSVNTATTTTTSSSIVSPLQSNSTINNSVDHFQFLKNSTDCLDLLDIILDRYLVPSLASSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3K192</accession>
    <name>E3K192_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tc1-like transposase DDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP78067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322486.1">
        <property type="nucleotide sequence ID" value="XM_003322438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K192"/>
    <dbReference type="STRING" id="418459.E3K192"/>
    <dbReference type="GeneID" id="10541537"/>
    <dbReference type="KEGG" id="pgr:PGTG_04023"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04023"/>
    <dbReference type="eggNOG" id="ENOG502RT6R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005726_6_0_1"/>
    <dbReference type="InParanoid" id="E3K192"/>
    <dbReference type="OrthoDB" id="2449121at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35871:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35871">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="899" mass="102541" checksum="EDEE27604268466A" modified="2011-01-11" version="1">MVVNKGRTAQRNRRAREALEKEQRSTPKKKSTNKRRSKLPKATKPAPQFSTDAPGTQSKPIPIDNFGDSKDVPVIVEDNELSESQQKEIENRNQANDVVQLLGTLYGDSESDIENDEDQDINETSDNDEILECLWPLFCNKKDLTLSSTRTLASGGTGYRKPVKNPNSSSQKLIPRPLPSSTKCDYKKNFKKAVGKNNDIMNNWLIKTTQEDSSPPVSDTVQHNCILPSCERTQAHISTNALECRLLEQLASYLESPNKVSAESKIRISANKKWAELNLAIISATATCQDKLKKNPKFRYPTGTIEDLHQFNNLRCQYTISGVKKPSLTASLETAISAIRRAPPPDPTKPPIPRSGIYHARLISSHAVYVLKFKEIKNTKIGNRTNHKSILDDIELRRELFQWALTQKIGEVTPISFRNHVLNHTFPTFKIEKTISRQTATAWMYKLGFSPQEYRKSIYFDGHEREDVVEARKAYIKKYHQLRRLSRVYGGENLELASPVDPEIIGDQKETVFIFHDESTVHTKERPGVAWLLPGTSEIWSKNSGRLIHISDFILESTDRLTSREDDTTPTDAATIIYPGSTGDKWWDMQQLCDQVTQKAIPIFNQTHPDSQAVFVFDCSSAHGAYSPSALRVQNMNLNFGGKQSWLHDSVIPSDDPHIPPHLRGQLQTFSYSVRVILEERGLWQHYSQEVSKLGLRPLKFHCEACKSSGLHKDATERSERLIRQAESNGFFLSQEQCIQEILQEEGSPNSAIPKPSADAINPGIPCCWFQVMQLQSDFQNERPLLQMIIEDAGHVCLFLPKFHCELNPIELFWSYIKQAYRQETHRCKTFSEYKALFERIRQSCPLSTIRKYFRRIDRQISAYEQGYNGPQSINLMKKYSSHRCIPRRAAMQIDMLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2D6</accession>
    <name>E3K2D6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_04461</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322924.1">
        <property type="nucleotide sequence ID" value="XM_003322876.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2D6"/>
    <dbReference type="GeneID" id="10536186"/>
    <dbReference type="KEGG" id="pgr:PGTG_04461"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04461"/>
    <dbReference type="HOGENOM" id="CLU_2122286_0_0_1"/>
    <dbReference type="InParanoid" id="E3K2D6"/>
    <dbReference type="OrthoDB" id="10486449at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP78505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="114" mass="12652" checksum="9CA953D37EC2588A" modified="2011-01-11" version="1">MGGAGHIQLHPAHPAQYELAHTRSVSGVVAEFRYAMLNANGNSAVLHYTNRYAQAEVVGVHLCQQTIMAGLREVIPILNPQLQPSSLSWKKELGELGGARNIQLHPFFHSKWRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3X9</accession>
    <name>E3K3X9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04737</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78781.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323200.2">
        <property type="nucleotide sequence ID" value="XM_003323152.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3X9"/>
    <dbReference type="GeneID" id="10536801"/>
    <dbReference type="KEGG" id="pgr:PGTG_04737"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04737"/>
    <dbReference type="HOGENOM" id="CLU_2039205_0_0_1"/>
    <dbReference type="InParanoid" id="E3K3X9"/>
    <dbReference type="OrthoDB" id="2510503at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78781.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="120" mass="13365" checksum="3D3362E8B39C1A72" modified="2012-04-18" version="2">MSFSRPMSIASSDATITGSGSDSDSVDESSSIDYGSVANYSPVDYDTVDKRSSAFPADYSNFSPAFTRDSFSLEELHDFRILVTDELIRFARSVSDSNETDRIQRLRLYSRVEDLFSTVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3K4C7</accession>
    <name>E3K4C7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Survival protein SurE-like phosphatase/nucleotidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_05726</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <text evidence="1">Belongs to the SurE nucleotidase family.</text>
    </comment>
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        <property type="protein sequence ID" value="EFP79405.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003323776.2"/>
    </dbReference>
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    <dbReference type="STRING" id="418459.E3K4C7"/>
    <dbReference type="GeneID" id="10531229"/>
    <dbReference type="KEGG" id="pgr:PGTG_05726"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05726"/>
    <dbReference type="eggNOG" id="ENOG502RXS5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045192_0_1_1"/>
    <dbReference type="InParanoid" id="E3K4C7"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Survival protein SurE-like phosphatase/nucleotidase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SurE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002828">
        <property type="entry name" value="SurE-like_Pase/nucleotidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036523">
        <property type="entry name" value="SurE-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30457">
        <property type="entry name" value="5'-NUCLEOTIDASE SURE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30457:SF0">
        <property type="entry name" value="PHOSPHATASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G01070)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01975">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64167">
        <property type="entry name" value="SurE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171747" description="Survival protein SurE-like phosphatase/nucleotidase domain-containing protein" evidence="5">
        <location>
            <begin position="31"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="domain" description="Survival protein SurE-like phosphatase/nucleotidase" evidence="6">
        <location>
            <begin position="33"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="327"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="69"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="327"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP79405.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="375" mass="40669" checksum="7E3B2D8E7D6C85CB" modified="2012-04-18" version="2">MGPSASSCSPSTLLMIIALFASFVIHPSSALNILQGNDDGWAECNIRTLYKVLKQRGHQTIISAPVRNKSGTGSRSTEWTPLREPGEYDTVPVGAPGVGPDPADPRVWYVNAFPLDGIRFGLDTLTPRFYHGMPDLIVTGPNVGKNTGLQDRFSGTLGAAAFGSRQGVAAIAISADDDNRHSYQTGDSEEKSSEIYAKATMRVLDEFILKMENTAEGPPNDFVPAGCVLNINLQRAGPQTDCRSAQDYRFVLTTIFGIRHQSGIDHCGSPFLPGEQAVLDSQNGCWAAVTLIQSHSFLDGSSEQKQALLDRAPEFFSCPAEELLKKELGPEMGVDRGTEPLPSFKNPSKEEQSDADDDDDKEEKRLYFQPGNISG</sequence>
</entry>
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    <accession>E3K4F4</accession>
    <name>E3K4F4_PUCGT</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05461</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79140.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323559.2">
        <property type="nucleotide sequence ID" value="XM_003323511.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K4F4"/>
    <dbReference type="GeneID" id="10530963"/>
    <dbReference type="KEGG" id="pgr:PGTG_05461"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05461"/>
    <dbReference type="HOGENOM" id="CLU_026173_0_0_1"/>
    <dbReference type="InParanoid" id="E3K4F4"/>
    <dbReference type="OrthoDB" id="10413477at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="389"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="545"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="117"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="396"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="547"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="557"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79140.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="598" mass="63451" checksum="5DCE834F8ACA8128" modified="2012-04-18" version="2">MTQRRSRRLFSQTENSYESRIQYLEEVVHILVAGSNSTCFPLFSTTPLASSFRYSIKRGLEPVLPDKTAISLAADWRIGRPIRQKFFSSPPRRQNRLITTCDTSLHPARVPSPTPPTSLTNPASPTTFGVANSANTAKSSLTSDLANKPTGFSPLDSPYSPLASVRLLDERPADTTSFLPEEALSPSPTPPYSPSTYPSITQTPSPDSARSSSPAPPQLTPTTPSDPPPHCQKRPIDIQRASALSISSSSPALPPLRSSTTPTNSALSSSRLPLHDCMKSTKAEDTSVTCSPASKISKSVSPAPLPLRSTTPSTPLATLYPLNSKINLHSATDSSPLTSTSDSMETLALLMPSEAHASHAITLTQRSNSANVAEPNDFLTLDNTPATPTAIIDVNPDPTTDTATTSAASSPHDHQLTSIPVTDGTIGPTSLSSLKLINNNDNQDNFLPTTNLAQSLTDTIDDTTTPLPVTSPVNPTLPLPDCSTPICPTAPACPAKLEIAGIGSLTVENDDSNSTDAQLAPEYSRTTLEYYNDIDNYLKLSGVNETKKKKKKKKKTTNSGSNSSTTSSAQPSDTLNTPEHLLPSLPVDVPAAALGALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3K4K9</accession>
    <name>E3K4K9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05757</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323855.1">
        <property type="nucleotide sequence ID" value="XM_003323807.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K4K9"/>
    <dbReference type="GeneID" id="10531263"/>
    <dbReference type="KEGG" id="pgr:PGTG_05757"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05757"/>
    <dbReference type="HOGENOM" id="CLU_1180710_0_0_1"/>
    <dbReference type="InParanoid" id="E3K4K9"/>
    <dbReference type="OrthoDB" id="10331176at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="222" mass="23451" checksum="3A6048A54E49A31E" modified="2011-01-11" version="1">MTNHCGRPPKHHAGPGLGHKGPMTQNPVVQSPTAHLENILKEENLATNQTSDTAAPRKNFLVTKPSFDTPALLKKFSATNSTFDEPAPLGSTSDTPALQKMSQSHPPALLEKFSASNPISDEPASLRAPLSLDSETPAPNKNILDSNPPFDPPAPLKKLSATNPMSDDPACLGSTSDAPAPSKNVLVTDPNFDSPAPLKKFSATDPMSDEPAPLENVTNPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3K593</accession>
    <name>E3K593_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
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    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003324060.2"/>
    </dbReference>
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    <dbReference type="GeneID" id="10532888"/>
    <dbReference type="KEGG" id="pgr:PGTG_06010"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06010"/>
    <dbReference type="HOGENOM" id="CLU_046741_0_0_1"/>
    <dbReference type="InParanoid" id="E3K593"/>
    <dbReference type="OrthoDB" id="2504751at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="255"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP79689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="360" mass="40036" checksum="E093917D253F41F9" modified="2011-01-11" version="1">MEKATAGLGIATLILFFCFHVVSVSSLAKRYDSRIVPVVSVLLTPIAGIALAFDILALCAISYEEMPDGIRAGAADIQNFLDPSIKALAAMLSFMSITTLTHILLHYLLRRRNSQHKKKKEARHQKAISSFSSSTIEDDKRSLESKEPLRRYLDTFIPSTLLAIIKTSLTLSPDNTIIERRILGLVEYITYVIVVVCCLRSRQAERVERDNFFGRITSNPSYARSTGSEELLVPPQIPRSHSILQRAPMASIGVGSYNSTSVVPRSVRPKPRERKSPPQTRNKLRMSYATQSAFSSLAPEDSASTAAFLPRPPPPPLHLRTRTSHGPNGSELAPLREDLVEKFNESAERFDQQTASYLTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K5D1</accession>
    <name>E3K5D1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06048</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79727.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003324098.2"/>
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    <dbReference type="AlphaFoldDB" id="E3K5D1"/>
    <dbReference type="GeneID" id="10533089"/>
    <dbReference type="KEGG" id="pgr:PGTG_06048"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06048"/>
    <dbReference type="HOGENOM" id="CLU_1166319_0_0_1"/>
    <dbReference type="InParanoid" id="E3K5D1"/>
    <dbReference type="OrthoDB" id="2511827at2759"/>
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    <proteinExistence type="predicted"/>
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        <location>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79727.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="238" mass="27364" checksum="639991D850ADB38D" modified="2012-04-18" version="2">MIFDKALNAYKLEAVKEMVLKPDPQELAKAYVEACAPEKVTTKHEGFKFPYQHFIDEFLSQIKIPQSIDPVSRGGIFNELENFDPRLSEIYRRPLVDGDMRLKDTPFEGLTADSRINLNNAAKMIYENWDTLNKALEERDQIPKIIKQMEDGFHEEIPRMIEIMKLIESNSRSPSPPIHPDETPKHIKMQRVNEEYQSRIKAKALPEQAKKVAEDDPAIQGKISETEDGSTKHSENNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3K5Q8</accession>
    <name>E3K5Q8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06132</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79811.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324230.2">
        <property type="nucleotide sequence ID" value="XM_003324182.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K5Q8"/>
    <dbReference type="GeneID" id="10533336"/>
    <dbReference type="KEGG" id="pgr:PGTG_06132"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06132"/>
    <dbReference type="eggNOG" id="ENOG502T02D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1147738_0_0_1"/>
    <dbReference type="InParanoid" id="E3K5Q8"/>
    <dbReference type="OrthoDB" id="2498133at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009571">
        <property type="entry name" value="SUR7/Rim9-like_fungi"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06687">
        <property type="entry name" value="SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="196"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="240"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79811.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="263" mass="27870" checksum="B5CF98ACC57601B1" modified="2012-04-18" version="2">MSEERTSIFIPRTSLRSPGFIGPVIVATALVVASIFTAPWDDDFSVIKIQDPTFVGFVGIWGACTRYNADNATTANPTATLTGNSTLDAMIHNSGKNRGCSRPGFGWTFSLPVNSTGLAGVPLPNSTIGFNVDQSENTSDQVWVEVMSKSQSHLLVTHVLAGIMATVGLLLLLIPYKPFEKTSPSLAKFIKTGSVSLILVALGAIASLVSFVLTLMTALKVRDTINQIGGGGGTHASLGNIFWFSIASFCLCVPSLWSTRGPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>E3K646</accession>
    <name>E3K646_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNA polymerase I enhancer binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_05938</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79617.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324036.2">
        <property type="nucleotide sequence ID" value="XM_003323988.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K646"/>
    <dbReference type="FunCoup" id="E3K646">
        <property type="interactions" value="198"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K646"/>
    <dbReference type="GeneID" id="10531452"/>
    <dbReference type="KEGG" id="pgr:PGTG_05938"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05938"/>
    <dbReference type="eggNOG" id="KOG0051">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_388374_0_0_1"/>
    <dbReference type="InParanoid" id="E3K646"/>
    <dbReference type="OrthoDB" id="2507391at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000390">
        <property type="entry name" value="YDR026C-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051651">
        <property type="entry name" value="DMTF1_DNA-bind_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017930">
        <property type="entry name" value="Myb_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46380">
        <property type="entry name" value="CYCLIN-D-BINDING MYB-LIKE TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46380:SF2">
        <property type="entry name" value="CYCLIN-D-BINDING MYB-LIKE TRANSCRIPTION FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13921">
        <property type="entry name" value="Myb_DNA-bind_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00249">
        <property type="entry name" value="Myb_DNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00717">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51294">
        <property type="entry name" value="HTH_MYB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50090">
        <property type="entry name" value="MYB_LIKE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH myb-type" evidence="6">
        <location>
            <begin position="458"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="458"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="domain" description="Myb-like" evidence="5">
        <location>
            <begin position="510"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="648"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="681"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="16"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="47"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="103"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="123"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="139"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="176"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="214"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="250"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="286"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="681"/>
            <end position="694"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP79617.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="711" mass="81151" checksum="EC0A3F435C9AF2B0" modified="2012-04-18" version="2">MQIETETNAVEEERKRQRKEQKKLKKLEKKRNRDQLAADQTEEPHQTVPTATGDPQSKLNQEVASKHKSKADRKLVDTTTDSPEKPMVDQEPQSKRQKTNHPPIERTTEPHRDSTSAPLQEAVEPKPTEALDKSPKEPLRKKKKNKERRKSKEKSGDQTDGPKASEIIPHNDNRVPESQSCTVNSPQTRVDSPAKAGTPTSKEALDGSTNSLVRQKKEKRKKSKEASGKTDDPTSSTVANNEGPVDQPLELQTSVPTQTSCEEAGDSMPPPEGLQNSLTEPNPPKELTKSKKKGPDKDRPTESMAPNDPVVRPSATKRPEGEVDPELDQILIDFKNSHLLEKAQLPEYFKTAIVHRTSREILTSKWLNITQLNELSTIFGLKYKKGRFSTVEQTMIEKMITKYCEEHRISKGEFGRMITQKKSNAARCKMKELMPSISDALPGRPLISIWKYIRRAYDPQAKLGRWTAEEEAALKDAHKKHGQSWTLISEQVGRPADDCRDRWRNHTCVKDLKNQGKWSQEEEDLLVQLMTQSKSIYSDPKDLQSDGLWTWVSNQMGGRRSRTQCRVKWVDSLQPKHANGGERGVWTNRDVLRLAQQLKKCDLGEDEVGFNWKQVKEGIEGWEIWNQGYLQRKWKSLKKYILKKHEKRWQKSDGSDDSSDPPPLSNQEIIDQAIQKWSARSEKALDTPVTRRVESQFPSEQVEGERSEDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3K6B9</accession>
    <name>E3K6B9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EFP79850.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324269.2">
        <property type="nucleotide sequence ID" value="XM_003324221.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K6B9"/>
    <dbReference type="STRING" id="418459.E3K6B9"/>
    <dbReference type="GeneID" id="10533378"/>
    <dbReference type="KEGG" id="pgr:PGTG_05075"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05075"/>
    <dbReference type="HOGENOM" id="CLU_1120596_0_0_1"/>
    <dbReference type="InParanoid" id="E3K6B9"/>
    <dbReference type="OrthoDB" id="1414216at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="216"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79850.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="248" mass="26410" checksum="FEA389C79B4D1613" modified="2012-04-18" version="2">MRYTKHALELWHVVYGPGCHPDASASVTHAGVLAQSTLDPSKSPRADSIFGLSQALIGSFFGTDSGKFAEATLILSQMFAQSGQKEQAIKFGLEAKKLYANRFGEDHDQTKQANQLHDSICRSIEAQIKGDEEKSNRLAKRLGLDPKRAASLRARLLASSSSATSAVSAESSLAQKTPSDQRLIGPQETLAPKIRPPVQNGSPVSIDDLVKYIQGSSSTKSSKATNGVNSPSASTSRTARQPSAVKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3K807</accession>
    <name>E3K807_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein CPL1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324640.1">
        <property type="nucleotide sequence ID" value="XM_003324592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K807"/>
    <dbReference type="GeneID" id="10534563"/>
    <dbReference type="KEGG" id="pgr:PGTG_06177"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06177"/>
    <dbReference type="HOGENOM" id="CLU_918709_0_0_1"/>
    <dbReference type="InParanoid" id="E3K807"/>
    <dbReference type="OMA" id="ANSHAIC"/>
    <dbReference type="OrthoDB" id="439917at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048661">
        <property type="entry name" value="CPL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038955">
        <property type="entry name" value="PriA/CPL1_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35192:SF2">
        <property type="entry name" value="APPLE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35192">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21671">
        <property type="entry name" value="CPL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein CPL1-like" evidence="1">
        <location>
            <begin position="246"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF21671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP80221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="342" mass="35122" checksum="2F912A5791C67744" modified="2011-01-11" version="1">MAPSATGTHLYARQLFPGQFLPGQLTAGQHTAAQVSAGTAQASAGCGLESNVWGQVSPVSASGYANLCLQCRVNVFGVEIFEFNFYNALAASISAHGVSASQVSVLQSAIDGDLLRLSAGSKTSASCTASCAGDQRCDSCSFVNGSCRMKAVNYLNQNRAGAPLAQAFQGLYSNGGSGYCSICPNLKDSVTCHFIASDRSCSGPKPSGLPRRSVAAKNPSVDERCPAGLSACPISSGLPLKLTAGFECLDVLQEVSSCGGCVSTGQGTDCTTIKGVSSAGCSEGKCKICKPLHSASIIVVVISIIDPPTYVPFGSLVSCKAGYQYSPVNNACYKSHRSTKMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>E3K877</accession>
    <name>E3K877_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="9">26S proteasome non-ATPase regulatory subunit 14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_06651</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M67A family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325114.1">
        <property type="nucleotide sequence ID" value="XM_003325066.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K877"/>
    <dbReference type="FunCoup" id="E3K877">
        <property type="interactions" value="674"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K877"/>
    <dbReference type="MEROPS" id="M67.A10"/>
    <dbReference type="GeneID" id="10539713"/>
    <dbReference type="KEGG" id="pgr:PGTG_06651"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06651"/>
    <dbReference type="eggNOG" id="KOG1555">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052991_0_1_1"/>
    <dbReference type="InParanoid" id="E3K877"/>
    <dbReference type="OMA" id="KTGRHEM"/>
    <dbReference type="OrthoDB" id="605656at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="C:nuclear periphery"/>
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        <property type="project" value="EnsemblFungi"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
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    </dbReference>
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        <property type="term" value="C:proteasome storage granule"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140492">
        <property type="term" value="F:metal-dependent deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:mitochondrial fission"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032436">
        <property type="term" value="P:positive regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902906">
        <property type="term" value="P:proteasome storage granule assembly"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd08069">
        <property type="entry name" value="MPN_RPN11_CSN5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.10:FF:000001">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000555">
        <property type="entry name" value="JAMM/MPN+_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050242">
        <property type="entry name" value="JAMM_MPN+_peptidase_M67A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037518">
        <property type="entry name" value="MPN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056263">
        <property type="entry name" value="RPN11_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10410">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01398">
        <property type="entry name" value="JAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23594">
        <property type="entry name" value="RPN11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00232">
        <property type="entry name" value="JAB_MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102712">
        <property type="entry name" value="JAB1/MPN domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50249">
        <property type="entry name" value="MPN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6 9" id="KW-0647">Proteasome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MPN" evidence="8">
        <location>
            <begin position="33"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP80695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="311" mass="34491" checksum="3EFB37511D1DDC2C" modified="2011-01-11" version="1">MDARAIQRMIQGAGGGNQTAGVDQPLNDTSEQVHISALALLKMLKHGRAGVPLEVMGLMLGEFVDDWTVRVVDVFAMPQSGTGVSVEAVDPVFQTKMMDMLNATGRPEMVVGWYHSHPGFGCWLSSVDTNTQQSFEQLTPRAVAVVVDPIQSVRGKVVIDAFRLITPQTIMMGQEPRQSTSNIGHLNKPSIQALIHGLNRHYYSIAINYRKTELEQAMLLNLHKKDWTEGLRLQDFEVHRANNEKTVKRMLTLASSYNKSVQEESTLTAAQLKTRHVGKQDPKRHLEEEVEAAMSDQISQALGTMMMALSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3K8I2</accession>
    <name>E3K8I2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar protein sorting-associated protein 62</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06466</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80510.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324929.2">
        <property type="nucleotide sequence ID" value="XM_003324881.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K8I2"/>
    <dbReference type="GeneID" id="10539521"/>
    <dbReference type="KEGG" id="pgr:PGTG_06466"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06466"/>
    <dbReference type="HOGENOM" id="CLU_024079_1_0_1"/>
    <dbReference type="InParanoid" id="E3K8I2"/>
    <dbReference type="OrthoDB" id="188042at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48171">
        <property type="entry name" value="DUF946 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48171:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173089" description="Vacuolar protein sorting-associated protein 62" evidence="1">
        <location>
            <begin position="21"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP80510.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="413" mass="46563" checksum="18149FB0EBCFC13A" modified="2012-04-18" version="2">MLLNAHLSLLTRNILTFLIARTFQVLTTTIPANGHLPYFTANQTLPQFALDFAPLVYLHSDEVFWPSDLNQHFDNVQPQINFTTLESYLVSAQTTQDFLGSNNLQSIEPTQQEITYLSSKQPIFDLDKASWLRGVGPPDQSGRSAAASIIIAVDKTQQVGPGWVDVFYLFFYSYNRGNYFYNNRFGDHVADWENTMIRFKDGKPMYVTPEAHGGQVMLGNSAFKYEVLERVNGRPVVYSANGTHGMYPQAGQQNYTGLPVAIYDVTDRGHLWDPKMNYKSFFFSNENGFSYTADTPVEQQDPQKLGWLKFLGYWGDQKLEPSNPHQTCIGPMCFYEGGPLGPQSKSLSRGWICGSSDCKPESSLPTILRPGGQRYNSPYSSSSTTRSQYSFSGLILRSLISYVAIHILFFHTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3K935</accession>
    <name>E3K935_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_06528</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80572.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324991.2">
        <property type="nucleotide sequence ID" value="XM_003324943.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K935"/>
    <dbReference type="STRING" id="418459.E3K935"/>
    <dbReference type="GeneID" id="10539583"/>
    <dbReference type="KEGG" id="pgr:PGTG_06528"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06528"/>
    <dbReference type="eggNOG" id="KOG0123">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_609932_0_0_1"/>
    <dbReference type="InParanoid" id="E3K935"/>
    <dbReference type="OrthoDB" id="19742at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000933">
        <property type="entry name" value="polyadenylate-binding protein 1-like isoform X5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002343">
        <property type="entry name" value="Hud_Sxl_RNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24012">
        <property type="entry name" value="RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00961">
        <property type="entry name" value="HUDSXLRNA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="173"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="262"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="354"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP80572.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="449" mass="49165" checksum="93723741D13F1E8F" modified="2012-04-18" version="2">MKNSHMYYSSSAAASGAPMSLPTRRRSQSAPEYLTRRAHVPVGHCALASQTHSITSSKNPSIRNRSTTASAAIVSSPTRYCSRSAVECRGPPTLAGYSVFTSQTRSLNNTRNTCSLNDISSCVPSTAPLVLPAHLPCLQPPVVNSAIAGISSACTPLNHFPPQHASRTTSVDHSVFVGDLPGSITHEQIVKVFSQVSPILKISIRYPRSQAIKKLRAYAYVTYHSAEDASTAIREFNQTVFEGKPCRVVRVQHDDLKSNKEANIYVGGLPPSLTGVAFHDTFSEFGEILSSKLVVNPKNVSNPFGFIQYATAEEAEIAIQKTNGATLDMKDSTRPISTSLFEGQAKPPRQPPFHNVYFKNIPKDITRRCFDQFCEEFGPTTSTFLKKDADGKPMGIGFANFSNTNDAVKIVEYIRQYAPMGLHAARAYTRGERNRYQSRRVPKTAKAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="93" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAR8</accession>
    <name>E3KAR8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Phospholipid-transporting ATPase</fullName>
            <ecNumber evidence="19">7.6.2.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">PGTG_06808</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 19">
            <text>ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2.</text>
            <dbReference type="EC" id="7.6.2.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-L-serine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phospho-L-serine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:38567"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:38568"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(out) + ATP + H2O = a 1,2-diacyl-sn-glycero-3-phosphoethanolamine(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66132"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:64612"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="14">
            <dbReference type="Rhea" id="RHEA:66133"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 18">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Membrane</location>
            <topology evidence="19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily.</text>
    </comment>
    <dbReference type="EC" id="7.6.2.1" evidence="19"/>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81187.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325606.2">
        <property type="nucleotide sequence ID" value="XM_003325558.2"/>
    </dbReference>
    <dbReference type="FunCoup" id="E3KAR8">
        <property type="interactions" value="132"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KAR8"/>
    <dbReference type="GeneID" id="10536586"/>
    <dbReference type="KEGG" id="pgr:PGTG_06808"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06808"/>
    <dbReference type="eggNOG" id="KOG0206">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000846_3_0_1"/>
    <dbReference type="InParanoid" id="E3KAR8"/>
    <dbReference type="OrthoDB" id="377733at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990530">
        <property type="term" value="C:Cdc50p-Drs2p complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140326">
        <property type="term" value="F:ATPase-coupled intramembrane lipid transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140345">
        <property type="term" value="F:phosphatidylcholine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090555">
        <property type="term" value="F:phosphatidylethanolamine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070273">
        <property type="term" value="F:phosphatidylinositol-4-phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140346">
        <property type="term" value="F:phosphatidylserine flippase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090556">
        <property type="term" value="F:phosphatidylserine floppase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032456">
        <property type="term" value="P:endocytic recycling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045332">
        <property type="term" value="P:phospholipid translocation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006892">
        <property type="term" value="P:post-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02073">
        <property type="entry name" value="P-type_ATPase_APLT_Dnf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000026">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000219">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000010">
        <property type="entry name" value="Phospholipid-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006539">
        <property type="entry name" value="P-type_ATPase_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032631">
        <property type="entry name" value="P-type_ATPase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032630">
        <property type="entry name" value="P_typ_ATPase_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01652">
        <property type="entry name" value="ATPase-Plipid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092:SF150">
        <property type="entry name" value="PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24092">
        <property type="entry name" value="PROBABLE PHOSPHOLIPID-TRANSPORTING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16212">
        <property type="entry name" value="PhoLip_ATPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16209">
        <property type="entry name" value="PhoLip_ATPase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 17 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 18 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 17 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 19" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="599"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="650"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1154"/>
            <end position="1174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1194"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1237"/>
            <end position="1259"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1274"/>
            <end position="1295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1302"/>
            <end position="1325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="1345"/>
            <end position="1364"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase N-terminal" evidence="22">
        <location>
            <begin position="335"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="21">
        <location>
            <begin position="429"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase C-terminal" evidence="23">
        <location>
            <begin position="1123"/>
            <end position="1375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="287"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="25"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="66"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="20">
        <location>
            <begin position="134"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="189"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="20">
        <location>
            <begin position="213"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="224"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="20">
        <location>
            <begin position="247"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="active site" description="4-aspartylphosphate intermediate" evidence="16">
        <location>
            <position position="715"/>
        </location>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="715"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="715"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
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        <location>
            <position position="716"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
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    <feature type="binding site" evidence="17">
        <location>
            <position position="717"/>
        </location>
        <ligand>
            <name>ATP</name>
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        </ligand>
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        <location>
            <position position="717"/>
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        <ligand>
            <name>Mg(2+)</name>
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        <location>
            <position position="800"/>
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        <ligand>
            <name>ATP</name>
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        </ligand>
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        <location>
            <position position="841"/>
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        <ligand>
            <name>ATP</name>
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        <location>
            <position position="864"/>
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        <ligand>
            <name>ATP</name>
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        <location>
            <position position="897"/>
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        <ligand>
            <name>ATP</name>
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            <position position="977"/>
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            <name>ATP</name>
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            <name>ATP</name>
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        <ligand>
            <name>ATP</name>
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        <location>
            <position position="1070"/>
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            <name>ATP</name>
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            <name>ATP</name>
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            <position position="1097"/>
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        <ligand>
            <name>Mg(2+)</name>
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            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
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        </location>
        <ligand>
            <name>ATP</name>
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        </location>
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            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004166"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00034036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00051303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR606539-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF16209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF16212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="EFP81187.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1442" mass="162530" checksum="F3FFFC75615ED8F5" modified="2012-04-18" version="2">MPSHILGEEEDFINSSDDELAHFSTTTTQQQQQQQQQQQQQRPTRTTNNNNNNNSGLGHNIDAIQLQHSTYHSSSHSPDPYTPQLLDGFQTNHHFPLSHQPTPPYGYYPHHSALPDHDDEEEEDDLRRGAGAAAEEEEQALELEQEEEEEEDQDYNQSSYHDHLQPPHSPTFDQDQNPAAYGGGHPSNPRGTMQDLNSSPTQPIYNPLYDDQSPSSINSNSYSAQPFNTTFNQSRNPQKLIPKNNHHPQSLSNSLTAAHHRSTSLSSKYAPDEYDDQNIIHGIRSIPLVDNHPPTSKHSFPKKQPSPKKSVPLKERLEQLFSRQPKELTGERLLYLNDAPRNQREFKFISNHVSTTKYNIITFLPKFLLEQFSKYANLFFLFTACIQQIPNVSPTNPYTTIAPLTLVLLVAAFKEMTEDIKRGKSDAELNTRAANILSGDSYIKKPWQDIKVGDVVRLESNEHFPADLILLSSSEPDGLAYIETSNLDGETNLKIKQANPSTAHLTSPQLASSIRGQLRSEQPNNSLYTYEGTMTLETSQMPQKQISISPDQMLLRGAQLRNTAWMYGLVVFTGHETKLMRNATAAPIKRTAVERMVNVQIVFLFIILLVLSVGSSIGSFIRTYSLGGQLWYIMQADSGKDKTTSFIEDILTFIILYNNLIPISLIVTMEVVKYQQAALINSDLDMYYPVTDTAALCRTSSLVEELGQIDYVFSDKTGTLTRNVMEFRQCSIAGVPYSDVVDENRKGEIFPFSDLPSVLAKNNDCGKVTNEFLTLLATCHTVIPEEKDGKIVYQASSPDEAALVAGAEVLNYRFKVRKPQSIMIEANGLQQEYQVLNILEFNSTRKRMSSIIRAPNGRIILYCKGADTVILERCAPHQPYKENTLIHLEEYATEGLRTLCIAMREIPEEEYQPWAAIYERAAATVNGRTEEIDKASELIEKNLFLLGATAIEDKLQEGVPDTIYTLQQAGIKVWVLTGDRQETAINIGLSCRLISESMNLVIVNEESADATADFIHKRLLALRAASKNPADSEDLALIIDGKSLGFALDKSISKPFLELAVLCKAVVCCRVSPLQKALVVKLVKKNIKGSITLAIGDGANDVSMIQAAHVGIGISGVEGLQAARSADVAISQFRFLKKLLLVHGTWSYVRLTKLILYSFYKNITLYLIGFYFSFANGFSGQVLFESWTLTFYNVLFTVMPPFVLGVLDQFVSARMLDRYPELYTLGQRNIFFTRRIFWEWVATAVYHSVFIFFVTALIFKDDLILHQGWISGQWLWGTTTYLVTLLTVLGKAAIISDLWTKWTLLAIPGSFALTLLLLPTYASIAPHIGVSKEYYNLMPRMLSSSVFYFCLLLIPVGCLARDLAWKGYKRLFRPEAYHIVQEIQKFNLPDYRPRMEQFQKAIKKVRAVQRLRRNRGFAFSQTEEGQEQLIRSYDTTVKKAKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KB10</accession>
    <name>E3KB10_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_07802</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325972.1">
        <property type="nucleotide sequence ID" value="XM_003325924.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KB10"/>
    <dbReference type="GeneID" id="10532934"/>
    <dbReference type="KEGG" id="pgr:PGTG_07802"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07802"/>
    <dbReference type="eggNOG" id="ENOG502T0M8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110357_0_0_1"/>
    <dbReference type="InParanoid" id="E3KB10"/>
    <dbReference type="OrthoDB" id="10288452at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP81553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="149" mass="17690" checksum="A81DE8DA2816C736" modified="2011-01-11" version="1">MLREGYHHACNYRRLSPEDLRQRYPHGYLSFDGYGDMICRDWPRLEQIFGPPDDLPLDASQPALSSREMIKDNSDAASQIVDFVDRINNQYHQTMQETPGFQRWSTYFNNNRSDRADYPLLQGLDNEVILRRYYNLTMGSNRNRPDFPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3KB95</accession>
    <name>E3KB95_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cytochrome c oxidase assembly protein COX16, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Cytochrome c oxidase assembly protein cox16, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_07857</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for the assembly of the mitochondrial respiratory chain complex IV (CIV), also known as cytochrome c oxidase. May participate in merging the COX1 and COX2 assembly lines.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the COX16 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81608.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326027.2">
        <property type="nucleotide sequence ID" value="XM_003325979.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KB95"/>
    <dbReference type="STRING" id="418459.E3KB95"/>
    <dbReference type="GeneID" id="10533151"/>
    <dbReference type="KEGG" id="pgr:PGTG_07857"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07857"/>
    <dbReference type="HOGENOM" id="CLU_131611_0_0_1"/>
    <dbReference type="InParanoid" id="E3KB95"/>
    <dbReference type="OrthoDB" id="5516033at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020164">
        <property type="entry name" value="Cyt_c_Oxase_assmbl_COX16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17130:SF14">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX16 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17130">
        <property type="entry name" value="MITOCHONDRIAL OUTER MEMBRANE PROTEIN 25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14138">
        <property type="entry name" value="COX16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="92"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="123"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP81608.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="161" mass="18704" checksum="DBB0910884D1F45E" modified="2012-04-18" version="2">MGLLPRKTLESQQNPYLQFLIRHVRQRPVIFFGFPFISTLLVGSVMLSRLTQTRYDYQATRVQSLTHADKLKLDQDQANRKPFDLREEYFKLSQPSAPQISEVSQIPSLSDENKKQPKSKRLKNWEEWDGEQVRVQRPLGAPEWGGVDPKQQSPEIGTRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3KB97</accession>
    <name>E3KB97_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Oligopeptide transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_07859</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81610.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326029.2">
        <property type="nucleotide sequence ID" value="XM_003325981.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KB97"/>
    <dbReference type="GeneID" id="10533153"/>
    <dbReference type="KEGG" id="pgr:PGTG_07859"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07859"/>
    <dbReference type="eggNOG" id="KOG2262">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004965_3_0_1"/>
    <dbReference type="InParanoid" id="E3KB97"/>
    <dbReference type="OrthoDB" id="2496739at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004648">
        <property type="entry name" value="Oligpept_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22601">
        <property type="entry name" value="ISP4 LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="349"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="482"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="513"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="591"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="662"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="694"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="738"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP81610.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="798" mass="88013" checksum="7BCA54288F629AD5" modified="2012-04-18" version="2">MGSDDDDKLTDLPDLMKSQSAIRTHRLSYLPPSTLSERSQRRHTTGRISFGELTSVPKIPAHRPKSLECVPEIRDTREKRPSFYLARTPETTDEFVVLDPPASVTDEATLFPLQDDPAELSITIRSSLTGIIMGIIGSAVGLLFIFKPVMVYLAPVFLQIMCMFFGRILALIPGPKWWNPGAFSLKETVYSSVISTAARNVAYSIVMLATEDLYFDQKSTPLRSMGILLSTSMVGYGWGYLIAPVLVHSSATIFPHILSSAALFYGVSGTGEYPKKQISLFHKVFAGISLYEIIPTYLMPALQGVSFPCLVLPASTLITSLFGGVAPFEGLGFFELSFDWNIVGAGSPLATPLFAQALQLLSVIITALIFWPAYSFSWFGFGQEEEFPFLSVSLFQENGTLYPIKQVTKNITKLDQNQISLPIYTATNVLTQIFQSLAVSSSITHILLWQWPLIRTAFKSRTTNDKEKDPHRLLVQASYKDFPVWASLACLGSATVWAFFLCYKENIITPFSLLISIGLSGILTLSMGFICAISGYTLPLNGVCQMIGGLIFRDNVMGNMWFSNYSSAPTSQALSAMSEMKLGQYTHMPPISVATAQATGMFVGVIVNYFVLEGLLSSQREALLLPNGDGIYSGTIVQNYGASAIAWGKFSHDLYTWGGRYVSVPLALGCGLCLPIPFYLLFKLWPHRYIKDLNIPIVMGFVALGSLGVSAGRTMNILVGLASQLWARRYYPKWFYKYNYVLSAALDGGTQIIILLLSLTVQGGVGPRLKLQIPTYFLNPPHPLPRDFCYLKNSKANS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBG9</accession>
    <name>E3KBG9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_07724</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81475.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325894.2">
        <property type="nucleotide sequence ID" value="XM_003325846.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KBG9"/>
    <dbReference type="GeneID" id="10537221"/>
    <dbReference type="KEGG" id="pgr:PGTG_07724"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07724"/>
    <dbReference type="HOGENOM" id="CLU_426494_0_0_1"/>
    <dbReference type="InParanoid" id="E3KBG9"/>
    <dbReference type="OrthoDB" id="3202072at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022698">
        <property type="entry name" value="OrsD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12013">
        <property type="entry name" value="OrsD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="142"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP81475.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="642" mass="73652" checksum="F2C71EDC4155EAE1" modified="2012-04-18" version="2">MRHWGSLDKDNGRFACPTEGCLESTEDPVRIQRHVKNCDPARFQQPRVMTVPPRDFVTLVPPGDQLVDIVICTTCHTGLDTPEVQAHLFNAHKEKVRQEDVILNFSSHGVSLPSTAPKYPVSRSGRLDLCQPVEGLFMYLGYICTADNHGEQCGHTVRKQDSMKNHFRLEHTDSNGHWKDNCKRSYVQHLVHKKRYFCVDRVPAAPTVISGILEGDEWRPQTMDTPLTERWEQLKDALSNLDVFLVPEANANTDVHASLLYDLVNYLDEMDDWIKYVEGWMLTRMNSLKEAHYGLRKEGLKESTEKEAPTVGLSPLQEKQTRSRYARTIAKMLLFTWKTFDTPLAAFWSRRQLRLKTTLASLSRCVGNPLPSHETINQLLDLAMFQYVGFSCSLAKLSRVQSAVEQFIALSMIQEDGSFFAPLELTHNIAAVQYGIRLGLTFHWVALASEMDDEELEEDEEQDLSNDAEMAKMRQHFSWISQDSRASPFMTLRDWMRLGSTFIMNEQVPDETHWVDSEMTRLMIGPSTITINGVQTSLRAVITSLRTEFQNVLKGVTLPDFLATELVDDSRNTSLHYNYLVPSTNEQLYRLKVLKDWTLNGNPQKVLAPNWEEVIQVPNPNLKRLWAPGPAWRWLRKNRYLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBM4</accession>
    <name>E3KBM4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_07758</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81509.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325928.2">
        <property type="nucleotide sequence ID" value="XM_003325880.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KBM4"/>
    <dbReference type="STRING" id="418459.E3KBM4"/>
    <dbReference type="GeneID" id="10537247"/>
    <dbReference type="KEGG" id="pgr:PGTG_07758"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07758"/>
    <dbReference type="HOGENOM" id="CLU_495345_0_0_1"/>
    <dbReference type="InParanoid" id="E3KBM4"/>
    <dbReference type="OrthoDB" id="10587659at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173415" evidence="2">
        <location>
            <begin position="26"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="356"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="518"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="356"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="372"/>
            <end position="382"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP81509.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="550" mass="50982" checksum="FB6E366219FAF743" modified="2012-04-18" version="2">MFKLKLSKLLIVVIAACQLFRSASCNQFHQNFKSQNLRRREVPFGNPNSNYANRNANDGYGPSTGRGNKVNNVPGQAGGAKYPGSSGPVPSGGNAVNAPNLGGILPGSAPGVANGGIGGSLPGVTNSGGISTGLPSSGLPTGGNGVNAPSVGGNPRYLSISERLVGNVKAHLLYPLSATSAPGKLPVGVNGGGLGNNAPGGIPGAPLGSPVVNSPGGTKGTSLGGVLGTTPGGSLGVPGSSPGGAKVTIPGGVSGTTTGTTTPGTTISAPGVGGNPSAAGAPGNLPVGLNGGGLGNNAPGIIPGAPLGSSVLNSPGGVQGTIPGGVPGTTPDGSVGVPGSSPGGTKVPIPGDVSGITTGTATPGITLGGLSGSPSNTGNNPSIPLGSTPGGSLGKNPGSISGIAAGSVPGSGLGSAPITPPGVVVGNVPGGSPGRIPGSGVAGSPVVPGGSPINNVGVISGNNPGSSAGGLLTNSTGKITVISSAAVSQNAPGTAGATNGATPPGTINSGVGNLGTSPVTPILGSTPQGASFNSTAAGLPFNQGNLSKPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCQ1</accession>
    <name>E3KCQ1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">G-protein coupled receptors family 1 profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_07600</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82203.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326622.2">
        <property type="nucleotide sequence ID" value="XM_003326574.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCQ1"/>
    <dbReference type="GeneID" id="10529575"/>
    <dbReference type="KEGG" id="pgr:PGTG_07600"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07600"/>
    <dbReference type="HOGENOM" id="CLU_871775_0_0_1"/>
    <dbReference type="InParanoid" id="E3KCQ1"/>
    <dbReference type="OrthoDB" id="2499150at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="64"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="176"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="226"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP82203.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="34468" checksum="B48A50A8B964865C" modified="2012-04-18" version="2">MSSSSDPSFSAPGSSDPGPHMMNPRLNLDSSVYVVPALTFLSCVAIMQYGNFCLTPASERSRQSFYLATIGISIHLVQIALDLWYMCYSFQSYLTGQTKDPVIFLYTTLEVCTAVGIAVTVQYHYFRLVQAMPALSKWWTVAIGFFAVATCVGGLGTGAEFQVMGRTPPPTAGPPVISIVFLAFYNMWLISNCLTDGTISIAMILALRHSRSPIRHQSLATTLRRLLFLTVSTFGLTFIVALLSLIVPALPHMIPSMSIRGQEICRVGSFVLNGILPRIYLNSFFCSLQCARGTKSAKHDTKTITDSEPDYEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCZ1</accession>
    <name>E3KCZ1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_07455</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326477.1">
        <property type="nucleotide sequence ID" value="XM_003326429.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCZ1"/>
    <dbReference type="GeneID" id="10534426"/>
    <dbReference type="KEGG" id="pgr:PGTG_07455"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07455"/>
    <dbReference type="eggNOG" id="KOG0247">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3160210_0_0_1"/>
    <dbReference type="InParanoid" id="E3KCZ1"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP82058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="48" mass="5603" checksum="9484B357DFAEB2C3" modified="2011-01-11" version="1">MHSWSDTENSHVIKFSTWQAKLSTSEKSDMVIPKFCLKIKLNLQLHQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDR2</accession>
    <name>E3KDR2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrotransposon Copia-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08454</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326917.1">
        <property type="nucleotide sequence ID" value="XM_003326869.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDR2"/>
    <dbReference type="GeneID" id="10529877"/>
    <dbReference type="KEGG" id="pgr:PGTG_08454"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08454"/>
    <dbReference type="HOGENOM" id="CLU_1152237_0_0_1"/>
    <dbReference type="InParanoid" id="E3KDR2"/>
    <dbReference type="OrthoDB" id="10286863at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="206"/>
            <end position="260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="260" mass="30057" checksum="FA588CA9D54DFE30" modified="2011-01-11" version="1">MTSGEENAEHYFTVEKLNGPNFWRWRNNILMLLTYLDLDDLVLSANPSVSGDGNNTAKKKQAAAIIRQHLDDQNAIRFVDDPSKFEPKELWDAICGHYAAPTRANAFELMDRLYGIKFVEGSFQESITEIRETSRFLFEVSQSYFDRKTLELHWIFFIRTRLPACFRHVGSEMMKRDDGNESLDNYLLELELEIRRQERAQQIGLACAGKQPSPSSDQHRRRPPRAYCKNGVHNPHTHHSAANCNQLHPKKIGNQQGLRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDT5</accession>
    <name>E3KDT5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08476</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326939.1">
        <property type="nucleotide sequence ID" value="XM_003326891.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDT5"/>
    <dbReference type="GeneID" id="10529899"/>
    <dbReference type="KEGG" id="pgr:PGTG_08476"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08476"/>
    <dbReference type="eggNOG" id="ENOG502S4I8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_637904_0_0_1"/>
    <dbReference type="InParanoid" id="E3KDT5"/>
    <dbReference type="OMA" id="IWVELIS"/>
    <dbReference type="OrthoDB" id="19657at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="31"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="432" mass="48729" checksum="5052F0249AFD7D23" modified="2011-01-11" version="1">MPFVQVSAKVQLHYEISYCESGGSESSLRPWLMIVHPLLMDSSYVKSFSDQSVIRSSFNQILFDARHHGRSKNPPSKAIDLFTLAADFAIGLEKLQIPAVHALGTHCWSSEILLRMAAVFPTKVISLCLCSIPPPTLEPYIERAFQECFDSCAYPESVEEWDEGVGAVQWFNFGDPSLIDRDILDEWASVLVRRIPPSRSIDCCLCVWPFLGACKVSKPDHIPDGLRSEIKLPILILRGANNTVFTEAGCQGRLNELPAHEHSKVKTIEDAPLLCTKTHPRETAVCYMDWVKEILDGKYSQSMQAQNSTCWKSNLEKLALYCKNESIVERDPFQSDSYNRNTKEEVEFFGKILADLSTHQVETFSLFGGRAPERWTGASFKEMVPWRFSSRFDEARLSAQGPVLQHSKHDCEVEEIQIKTSVEVCSQDLVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3KG28</accession>
    <name>E3KG28_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Lysophospholipase</fullName>
            <ecNumber evidence="2 9">3.1.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_09252</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the lysophospholipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="2 9"/>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EFP83299.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327718.2">
        <property type="nucleotide sequence ID" value="XM_003327670.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KG28"/>
    <dbReference type="FunCoup" id="E3KG28">
        <property type="interactions" value="216"/>
    </dbReference>
    <dbReference type="GeneID" id="10545014"/>
    <dbReference type="KEGG" id="pgr:PGTG_09252"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09252"/>
    <dbReference type="eggNOG" id="KOG1325">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014602_0_0_1"/>
    <dbReference type="InParanoid" id="E3KG28"/>
    <dbReference type="OrthoDB" id="4084751at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000030">
        <property type="entry name" value="Lysophospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF33">
        <property type="entry name" value="LYSOPHOSPHOLIPASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8 9" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6 8 9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="33"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="domain" description="PLA2c" evidence="11">
        <location>
            <begin position="86"/>
            <end position="652"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP83299.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="653" mass="71886" checksum="74FF94D29791EBA0" modified="2012-04-18" version="2">MGIMPLNPNLKIAVNIPPPSVIARQRTCYRMSIALNLIVVITLFITIYASIFILIILATPDTPRLFARSPALTNSPSGGYAPVWVDCPTDLSVRQPTLAGPLASKEAEYITKRTLKSVPYWRSYLSRAGLSEFDIEAFLKKATTEGAVRGLTLPNIGLALSGGGPRAALIGASMVHALDDRNPDAVRAGTGGIIQLANYVTGLSGGSWVYRAHGFSTSNFPDVRELVSTWKLQQDNQPLDWETVKKYPPAVKIAREKANAGFPASLVDVWSLMVGKHFLNAPDYGKSVLFNSIRDVPAFQNYDAPFPIIVATSRGEKGRSSIDLQTPIYEFTPYEFGVWHPSMNGFIPIDYLGTSMFDGKTIPQSPCVKGFDNAAFIMGSSSNILSEPFAPNEKMTLWNKIVAGLYEYFTRHMYDEALVFNPFNGLGTGSSPKQEFPDGKDTLLYLADGGLGGENMPLWPLMQPARNLDVIIAVDAQTDGVGYTIDARGYSNGTSLYMSYKKTLLPDYKGYKFPKIPDYRSKFSELGLHQRPSFFGCNETDAPLVIYFPNYYMVAETDFTTVQASYTQAEVDAVFDNGFAIATQSIGKIKDNTLLSQGIEENIQRAGKYATPDWSTCVACTLIDRQLQRDGIPRTSQCEACFRQHCYVESSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3KG49</accession>
    <name>E3KG49_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Prolyl 4-hydroxylase alpha subunit Fe(2+) 2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_09261</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EFP83308.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327727.2">
        <property type="nucleotide sequence ID" value="XM_003327679.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KG49"/>
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    <dbReference type="KEGG" id="pgr:PGTG_09261"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09261"/>
    <dbReference type="eggNOG" id="ENOG502QWAB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007520_1_0_1"/>
    <dbReference type="InParanoid" id="E3KG49"/>
    <dbReference type="OrthoDB" id="27483at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33099">
        <property type="entry name" value="FE2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33099:SF7">
        <property type="entry name" value="MYND-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prolyl 4-hydroxylase alpha subunit Fe(2+) 2OG dioxygenase" evidence="2">
        <location>
            <begin position="171"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP83308.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="954" mass="106815" checksum="09DE9EFD1E1C6634" modified="2012-04-18" version="2">MSGNPDSNRDPGERASDNIDPALQHDSEPSASDNSDRSSSEDSSEDVDYQTAWKEDLINAFDAITTPGTFAAWEALPTTPPAGLYVDGVGEIAMPLGKEQVQQLIAKAHQAPFGRRSETLVDLSVRNTWELNGDQLRFLDPAWQSYLLDLSKRVATSLGVQGLIRLELYKMLIYEKGAMFKPHTDTERTPGMFGTLIICLPSMHVGGEVVVKHNGESKTLRTSDAAQSFACWYSDVTHEVLPVKSGYRCVLTYNLAIKPGLARPAASALDPQKKPLRKTLQRWLKDLTNDSSNDPPSHVYHALDYEYTEAAMSFKALKAEDFARVQVLRDLADELPFEIFLALLEKKASGPVESDWGGRHSYKRSRYDFDDDDDGSNSGHHTIDETETDYTIKSLRALDGTTIASNYDFDMDFCSEEDPFQDLEIAREDYESYQGNWGPSATHWYRRSALVIVPHKCLAEYLAECASDSKSTNPALLYLDHVCLRPSASKPMLDSLADLCKKQSNKPLPPGTIRNILKASFQHSHFALFQAIAGLHQGHLPLDFFDWAKEWLQTLPDVDRAEKYQQWYPSLIHSFPSVADRISIIEKVSNPTSNIAPLDGAPTSKAWVQDLTRQCVTKLLEDNIIPTAADGNAIVSAVFNLNETLTNTSALLTSIFDRFPQPEAVAFLLGFLSQLKTLAEGVAHFPTLENVELRKNLSSRVFNRQRTPSSVITRAKGKSASEIGRVVTPRALAKYACDLNSLSTDTADLLEPFVQQINEHCPKFTAQDMQKLWMPFLYRLIPALVSRSIQLNQPCHQQLARQFIKRYSDTTLGACPNPDIISRQSPQVRCTCLDCMELNRFLRNPSQRVCRFKATRSERFHLHQQIEGANIDCTHVTDRTGYSETLVVTKRSSLQDEVNGWKKRQKELYFEICNKIQPDHLESLVGPQEAANLRILAGLRPVAATSAPIVIDLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KG51</accession>
    <name>E3KG51_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EFP83310.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327729.2">
        <property type="nucleotide sequence ID" value="XM_003327681.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KG51"/>
    <dbReference type="GeneID" id="10545025"/>
    <dbReference type="KEGG" id="pgr:PGTG_09263"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09263"/>
    <dbReference type="HOGENOM" id="CLU_2623168_0_0_1"/>
    <dbReference type="InParanoid" id="E3KG51"/>
    <dbReference type="OrthoDB" id="10377935at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83310.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="78" mass="8685" checksum="B2708C9C3F087607" modified="2012-04-18" version="2">MREDELPMTFSIHSESRKPTETLDLLKEFGKYPMQDSSANLVESPMQAVSPHRATQTDGNSISGAYNAADDTRRLSQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3KG69</accession>
    <name>E3KG69_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Expansin-like EG45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09118</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EFP83165.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327584.2">
        <property type="nucleotide sequence ID" value="XM_003327536.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KG69"/>
    <dbReference type="GeneID" id="10544878"/>
    <dbReference type="KEGG" id="pgr:PGTG_09118"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09118"/>
    <dbReference type="eggNOG" id="ENOG502S9P9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026963_3_0_1"/>
    <dbReference type="InParanoid" id="E3KG69"/>
    <dbReference type="OrthoDB" id="2495887at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22271">
        <property type="entry name" value="DPBB_EXP_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051477">
        <property type="entry name" value="Expansin_CellWall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836:SF21">
        <property type="entry name" value="EXPANSIN-LIKE PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83165.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="235" mass="24645" checksum="62F02F2F0DBD04B6" modified="2012-04-18" version="2">MLFSSSNSFAGAVSVNTSLYGDGEQVFQGEATTWGANWPSGNCLFEKWPQPEGLGAIAMASNLWDSAGICGACISITGPSGDTHDGIVSDQCPSCKKDSLDLAPDLWKKVSNNQTPGILKISWKIVSCKFSTPIEFINKGGVSKDSTSVQVAGSNMPIESLEILPSGSNSSGTMPTGNWVKLVRQSSSNYFQPDPPSGLGAIGDLRVTCGDGKQIITKHVHLDQPLNTTAATGNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGA3</accession>
    <name>E3KGA3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Autophagy-related protein 16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_08514</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ATG16 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327747.1">
        <property type="nucleotide sequence ID" value="XM_003327699.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KGA3"/>
    <dbReference type="STRING" id="418459.E3KGA3"/>
    <dbReference type="GeneID" id="10545051"/>
    <dbReference type="KEGG" id="pgr:PGTG_08514"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08514"/>
    <dbReference type="eggNOG" id="ENOG502S5CU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_081023_1_1_1"/>
    <dbReference type="InParanoid" id="E3KGA3"/>
    <dbReference type="OMA" id="VQACSQM"/>
    <dbReference type="OrthoDB" id="8949486at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22887">
        <property type="entry name" value="Atg16_CCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045160">
        <property type="entry name" value="ATG16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013923">
        <property type="entry name" value="Autophagy-rel_prot_16_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19878">
        <property type="entry name" value="AUTOPHAGY PROTEIN 16-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19878:SF8">
        <property type="entry name" value="AUTOPHAGY-RELATED 16, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08614">
        <property type="entry name" value="ATG16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Autophagy-related protein 16" evidence="3">
        <location>
            <begin position="7"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="129"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP83328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="218" mass="24558" checksum="5F6B22C78DC20605" modified="2011-01-11" version="1">MSWQDVIQEGLVARDANEKAYNSIINQYRRLAQQTAILKERNSALLKAAGSIGGGGSRSTAGGGDSSVARAYTASLESQLSTLRDELATVYKTQGQNAQRLLVMNEALREREERSRAEVDETRLLRVEAARLRERVASHAEVMREKERNVQILQDELSTLQLEYTQQEQKIKDLKSDNANLLQRWLDKVHEEAVRMNDANQFLVEVEKKGLPPVEPPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGE1</accession>
    <name>E3KGE1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_08552</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83366.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327785.2">
        <property type="nucleotide sequence ID" value="XM_003327737.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KGE1"/>
    <dbReference type="STRING" id="418459.E3KGE1"/>
    <dbReference type="GeneID" id="10545082"/>
    <dbReference type="KEGG" id="pgr:PGTG_08552"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08552"/>
    <dbReference type="eggNOG" id="KOG2615">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_10_2_1"/>
    <dbReference type="InParanoid" id="E3KGE1"/>
    <dbReference type="OrthoDB" id="196650at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031526">
        <property type="term" value="C:brush border membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000223">
        <property type="entry name" value="Major facilitator superfamily domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23504:SF31">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY DOMAIN-CONTAINING PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="227"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="290"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="356"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="389"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="471"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="17"/>
            <end position="491"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP83366.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="508" mass="55394" checksum="C4F869B090BFEA26" modified="2012-04-18" version="2">MADNDHQEARLVLQRRVVKIIFFSLLLDLLAFTMPLPLFPRLIASFVEAERSHGDTTLLSSTLNTIRACRTALASWRSTPSSQMTVTSNSKWDVTILGGILGSIFSFCQFLISPVIGRLSDRFGRRPVLLMTMIGNIFSAFLWLTSTSFGPYVLSRAVGGLSEGNVQLSIAVISDVSDPTSRAKSLALVGIAFSLCFTFGPMLGAWFAMRSPPLQATKLLGIGLNVYAVPALISLLLLLLETLYLAIALPETKLLRNAGKLGSKPKSNHVNDQKSAPGSSFEQRQLRLRILAQVHTAFLFFFSGAEFTLTFLTYDLYGFTNAQNGRLLGFIGILSSLLQGGYTRRSKKPPLWFVKSGLKACISSLVLLSVLPFLRPEVGERVEGTIDRLSALFLYSAAAALAFVSASVVNSLNTLASLECDEDDDDDQQSTGARKIARGKALGDFRSAGQLGRAFGPLFATGLYWTKGPSLCYSLCAIGTFAVFFLTNQFNHLQPRLPPTPVHPKKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3KH23</accession>
    <name>E3KH23_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">BRCT domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_09311</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83598.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328017.2">
        <property type="nucleotide sequence ID" value="XM_003327969.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KH23"/>
    <dbReference type="STRING" id="418459.E3KH23"/>
    <dbReference type="GeneID" id="10532282"/>
    <dbReference type="KEGG" id="pgr:PGTG_09311"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09311"/>
    <dbReference type="HOGENOM" id="CLU_535432_0_0_1"/>
    <dbReference type="InParanoid" id="E3KH23"/>
    <dbReference type="OrthoDB" id="2501940at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10190">
        <property type="entry name" value="BRCT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001357">
        <property type="entry name" value="BRCT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036420">
        <property type="entry name" value="BRCT_dom_sf"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50172">
        <property type="entry name" value="BRCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BRCT" evidence="2">
        <location>
            <begin position="424"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="517"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="530"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP83598.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="597" mass="66254" checksum="888419DF055182CD" modified="2012-04-18" version="2">MPGRLCSMSPLSPQPVTSECCQFTTLYKNSQNTSIPNLLTSSPQLASVNLSGNQIVPQIGVYPTTALHQMDPRPSQSSLKPSLKRSRCEPSTTSTSRKRVATANSSSDLLDSQSEELNEWQKASIAWKKSYDQKVAQCLARNQLPVGPVEQYMNLTSRIPVTEQSLDSTHNIPSPVISPLPQSPRSGVENLTEDHTDSADPNESSAPTILPQSLTRKTHSGRVLLAPETPDVSMVLQKSSAILAPETPGVPLLRPKSRIVLAPGTPASPSSSHQIKKAPSHIISPGLGIRPQISNQQQQVNHHHVSNRSDTSNSRGVHRTTSETSSSSGIKVKDTKLPRRRSKLKEIDTLEAYDKLTAKERSARFPSVEIWAAFLTESSRISAKRRKTKDFMSRCRIYYLIDPRVQRNLDDADRMRMRRLFEAGAQIQPELRAKQVTHLIVPNDTSWNSCLRAIKNLVADPVERQTIKDMCLSSLQKTPGDELIWVMDFKWVSSCLSYGAIPVEKYTCIRPRASKMVGSSKHTSMAPPESQDHNRSAEADHPNDTSSDEIETSVSLHPNMYPMLLAPFLGEYVESNRSMVEHSHSETQAKHLSPIYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHV9</accession>
    <name>E3KHV9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_09597</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EFP83884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328303.1">
        <property type="nucleotide sequence ID" value="XM_003328255.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHV9"/>
    <dbReference type="GeneID" id="10532573"/>
    <dbReference type="KEGG" id="pgr:PGTG_09597"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09597"/>
    <dbReference type="HOGENOM" id="CLU_1971584_0_0_1"/>
    <dbReference type="InParanoid" id="E3KHV9"/>
    <dbReference type="OrthoDB" id="2509385at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173407" evidence="2">
        <location>
            <begin position="32"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP83884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="134" mass="14854" checksum="D1679BAA69E6E126" modified="2011-01-11" version="1">MCRRRRSMFALIASVMVVCLMVQQVFNRGGALSSIGNGQAKFSFNTHVMKETVDHSKLHHLGEEAHRNQTDSASKTPVPKKPKPAPPFPDPFITTSCPTFKRGHSVVDQLISRKMNIHVKLEKCSPMEGMGSPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHX2</accession>
    <name>E3KHX2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_09610</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EFP83897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328316.1">
        <property type="nucleotide sequence ID" value="XM_003328268.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHX2"/>
    <dbReference type="GeneID" id="10532587"/>
    <dbReference type="KEGG" id="pgr:PGTG_09610"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09610"/>
    <dbReference type="HOGENOM" id="CLU_2062635_0_0_1"/>
    <dbReference type="InParanoid" id="E3KHX2"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP83897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="119" mass="13670" checksum="DAE4D4769CEE0B7D" modified="2011-01-11" version="1">MLGWLVIKGSLVIPPISRVSTSFILQGERCRFFISEVKSSKSLHWKYNFFKRFNIHFGKVYLVPLGCYNRLGLLCHQELMGKGHTIAKGASGNKPHLDTYKVEGLAPYNYKGQQWNELH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KI44</accession>
    <name>E3KI44_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_09682</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EFP83969.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328388.2">
        <property type="nucleotide sequence ID" value="XM_003328340.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KI44"/>
    <dbReference type="STRING" id="418459.E3KI44"/>
    <dbReference type="GeneID" id="10532661"/>
    <dbReference type="KEGG" id="pgr:PGTG_09682"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09682"/>
    <dbReference type="HOGENOM" id="CLU_045164_0_0_1"/>
    <dbReference type="InParanoid" id="E3KI44"/>
    <dbReference type="OrthoDB" id="2509788at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP83969.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="425" mass="48298" checksum="E391271627C190B8" modified="2012-04-18" version="2">MEHDDQQEASISSREGSIDLVREHFAQLMRRCEASQFPAPSDEPPPIIGISIDPFRLKEYLFDHLHSTLLPQLQEHILKLGALCELCHVRDDFGAIFQSVLEIQVDFDRVLEKIIAAKHKICLPDPGSDPNHDNYLNEFKHYRISILDKTLNKLLSRQLHSAFDTAQRFYLTDCIPPSRRSDSPDDYIRRRLTTDLAQSSNSIESTIQCMRGPEFYLIQVHWPIAIPILEKILEDYWQLIARYHCGTESEVSRIEPFIPMVQAIIPMVRLCRLLLIKVSKKISINQEFLPIFTHLPSRSLSILHNLPTKMARYLDGILHQFKGIIDPYRPPDELNLLGLIEDNSKIFTAALELLISHILPAISQDTNDYVACNDLNKIWSANLSVINYTAVAGGRQIAENSAVNLTDPAQPALTGKKRASGQPAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3KII1</accession>
    <name>E3KII1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_10484</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84106.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328525.2">
        <property type="nucleotide sequence ID" value="XM_003328477.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KII1"/>
    <dbReference type="STRING" id="418459.E3KII1"/>
    <dbReference type="GeneID" id="10545204"/>
    <dbReference type="KEGG" id="pgr:PGTG_10484"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10484"/>
    <dbReference type="eggNOG" id="ENOG502S8Y0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008138_0_0_1"/>
    <dbReference type="InParanoid" id="E3KII1"/>
    <dbReference type="OrthoDB" id="2508964at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266">
        <property type="entry name" value="CHROMOSOME 15, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266:SF1">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP84106.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="954" mass="107584" checksum="2968BC06C78387D8" modified="2012-04-18" version="2">MLDLVISLSRAAGLRHRLNFCTRHPQHCYVGLGLPHTISQPAVIVMADGEPATPCCSHQFPIYRSGPTTKLSSESYTIDIRSYLNAMPPARRSQRILQRSNADSLVERSKRINIHDVLHEDFLTFAQSVPPHLADKYLTILEEIFKHCEPKRTKSMGDIGVSRLIVFWASGKVVLTWKQVISVLKIQKNQSLQKKFVQLAFLRRYPDYYENDKIDHQARVAIIDDTLDHGLARLQSATEPNILPLDVIRKGFLSDYIGRDEIVTPILSTLNEHATSWTPEEYHAPCTSIIGPTMAGKTRLLMELADEICVVYICLRPPTSIGEPKRSQLATEMLKPPPCTDLETYYVDLITAILSVTIKFFQSASKTKDRKELLRAWYQHHNSPKTKFYSNVQSELRRLTSKNDTLRQLDLAAERLGKTHVLESSPLKVLLAIDEANSLFDERGTQTDSHGKQSQELPLSHFFRRALRHVPSMSGIFAILVDSDSRVANFNPSARIEVLPTNRPIGVRAQPSKMYPDIYELRTIDRMAPANPPRRWAQLFSPERLCKYGVPFFSLQLTLAVEDGRALDPTKAVHVVAGVALNKLLCAPMAGPRELTESRALALLGPTIGVPLHGQARLNAQLMASHAAHCGYIDAERHCHYSFYPSQPIYALAANHYLLENEEVLIMCINFLTGVLSEDHMSAGDVGEIASRIILLCAMNKTAADIKTAKQSSANPRSVGRISIPDPVPVIKFLETLTGLSAHELPLGSIDADHKHKLLEQGMMFWNHFMHCLERPTTESLLECLQRGLALHSQPSQEVFDQVLTIYLKEQSEDKLDEANVTFCGIRARNRKHDFELNTSQQIMNPETAKIDMKEKSNPYLSLYFTFQDTPLIEENPIRRDDYELPSHGPPDGRQASLVFYGLDSFHFLSPGLKNALKELINIRPDPVSRSRKRKAGEEYATDFLLRADARRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJ49</accession>
    <name>E3KJ49_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_10044</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178290">
        <property type="protein sequence ID" value="EFP84324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328743.1">
        <property type="nucleotide sequence ID" value="XM_003328695.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJ49"/>
    <dbReference type="GeneID" id="10545670"/>
    <dbReference type="KEGG" id="pgr:PGTG_10044"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10044"/>
    <dbReference type="HOGENOM" id="CLU_2027886_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJ49"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP84324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="122" mass="13996" checksum="37142DC73F23CF6A" modified="2011-01-11" version="1">MPLASCTVIQETGPSYQETRAWAVVRGNSWLPRTFDTGLTNVSDAYLVCSGKRGKHWEFCRLLLQSGKLGFNALLRAFWYSPPAEWGPTDQRLRTCNKYKLVADMVMLWVYDIDNFCGDDYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJX5</accession>
    <name>E3KJX5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10759</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84600.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329019.2">
        <property type="nucleotide sequence ID" value="XM_003328971.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJX5"/>
    <dbReference type="GeneID" id="10546269"/>
    <dbReference type="KEGG" id="pgr:PGTG_10759"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10759"/>
    <dbReference type="HOGENOM" id="CLU_702347_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJX5"/>
    <dbReference type="OrthoDB" id="2497736at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173949" evidence="1">
        <location>
            <begin position="17"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84600.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="393" mass="44918" checksum="F4888F54542BC172" modified="2012-04-18" version="2">MIGIFLFLILPYLIQASSVVPVLSAPAEKSENVCSKAGHARALTKRAFKPRVVKGAVKLPGDSRREIDNPIGRYPQQWPNCGNYKLRVSTKCGASQNPRVEHGLIHSILSTLDKKDRSTAENVEFMKHQLKRIASSGNISRPLGKGDGVASTFEINLLYFLLHCFEDKFIHRWFQIYDSWSSLNTPFLIEARADHDPSFLYAAINSISPGLWKFCLPMKDEKFDIDLLISNPIARLSWFLDGLDPAEMIEYAQALEKPQSPTLQELMNRQLILEKEDKTEPSQKFAQASWFAQRMGWDHFQRIILDFEKFTPNTNSYKFKMSGFCQPGTGVLSTPKARKSMQELNKLLEKKGFLTVSSDENFSSNIMEWLSTFLTWLNDSKVQINRFSTWEMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3KL23</accession>
    <name>E3KL23_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Wax synthase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_11167</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Secondary metabolite biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the wax synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP84998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329417.1">
        <property type="nucleotide sequence ID" value="XM_003329369.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KL23"/>
    <dbReference type="STRING" id="418459.E3KL23"/>
    <dbReference type="GeneID" id="10526975"/>
    <dbReference type="KEGG" id="pgr:PGTG_11167"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11167"/>
    <dbReference type="eggNOG" id="ENOG502SS5M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026730_0_0_1"/>
    <dbReference type="InParanoid" id="E3KL23"/>
    <dbReference type="OMA" id="FRAFEWS"/>
    <dbReference type="OrthoDB" id="1077582at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044851">
        <property type="entry name" value="Wax_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032805">
        <property type="entry name" value="Wax_synthase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31595">
        <property type="entry name" value="LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31595:SF57">
        <property type="entry name" value="OS04G0481900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13813">
        <property type="entry name" value="MBOAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="214"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="254"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="319"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="363"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="397"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="Wax synthase" evidence="9">
        <location>
            <begin position="291"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP84998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="426" mass="48187" checksum="76B946CD6C94C362" modified="2011-01-11" version="1">MIQDIRLIPTIPFAGTHGLLHALQVLLQILFGLFLGAMTIPSQSWPAQVFRLGLLVWLVPKILDYSTSRSYSFGNMLADQLMLSCFGWNLLTRLLELSLLPLISPAPPRWIRPTKEELARVLKLRHQPASNQHPHKLDQASEELIPTSWEPVPFPAPFSLDRVLYAYDYLMLIRPSTSDLLPWQFRAFDWSLPAMSAGGVAGRRFGKPETGPRLALVYLFVYLVCFVYVDNVAIRHIGQITVAELGLAHQLLLTISAGCLISLSAGPSEAFIFPLLLSRRILPPTALLASFNQPYLASSIQDFWGNRWHHRVRRQLTRLGSLFPLGGTRTGNAFWAYVLSAMLHSFIICRSKPEPSLSDPTSYVALFFDRPTVIFFVSQGLAMIVERHLVPPDLRRLWLWFTIIFAGRWYIDGILNSYLPPPPPTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3KL27</accession>
    <name>E3KL27_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_11171</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP85002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329421.1">
        <property type="nucleotide sequence ID" value="XM_003329373.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KL27"/>
    <dbReference type="GeneID" id="10526979"/>
    <dbReference type="KEGG" id="pgr:PGTG_11171"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11171"/>
    <dbReference type="eggNOG" id="ENOG502T1ND">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2172264_0_0_1"/>
    <dbReference type="InParanoid" id="E3KL27"/>
    <dbReference type="OMA" id="TTRYLPH"/>
    <dbReference type="OrthoDB" id="2501350at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024512">
        <property type="entry name" value="Ser_palmitoyltrfase_ssu-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11779">
        <property type="entry name" value="SPT_ssu-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP85002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="110" mass="13072" checksum="15AD92209631E608" modified="2011-01-11" version="1">MDKQDHSPSTLSRRSNHKSSSRTEARQTHQTLHPSFEIIRHKSFGTLMHELWIRFQGEFGLSMLETWEMVMIYIIVILLILLFYVAIFKYFPSHIKIIGRRAKYYLVGSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLB6</accession>
    <name>E3KLB6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11260</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP85091.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329510.2">
        <property type="nucleotide sequence ID" value="XM_003329462.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLB6"/>
    <dbReference type="GeneID" id="10527072"/>
    <dbReference type="KEGG" id="pgr:PGTG_11260"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11260"/>
    <dbReference type="HOGENOM" id="CLU_2016327_0_0_1"/>
    <dbReference type="InParanoid" id="E3KLB6"/>
    <dbReference type="OrthoDB" id="10268239at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173505" evidence="1">
        <location>
            <begin position="24"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85091.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="123" mass="13740" checksum="50C2EBC4AAC0AD98" modified="2012-04-18" version="2">MARFAWVLSLLLTSFLVAPGIQGPPTGPYRYTDRCERCWQAAGSPTDFVNTMYKTVRYSSYPCYAFGHDLTCRHKFAVQGYYCVGCCNLVHVSLGRCPTHGQSTQYIPEPRLTTPQAPRPAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLS7</accession>
    <name>E3KLS7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Kinesin motor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_11445</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="DS178294">
        <property type="protein sequence ID" value="EFP85276.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329695.2">
        <property type="nucleotide sequence ID" value="XM_003329647.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLS7"/>
    <dbReference type="GeneID" id="10527266"/>
    <dbReference type="KEGG" id="pgr:PGTG_11445"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11445"/>
    <dbReference type="HOGENOM" id="CLU_1866140_0_0_1"/>
    <dbReference type="InParanoid" id="E3KLS7"/>
    <dbReference type="OrthoDB" id="9971853at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007018">
        <property type="term" value="P:microtubule-based movement"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kinesin motor" evidence="3">
        <location>
            <begin position="32"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="26"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP85276.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="137" mass="15099" checksum="FF082A5A7EC56DF2" modified="2012-04-18" version="2">MAQGEKGDFAYFMQVHPAAPYPASHYHSLPIGSQTTPTDRKLNQSKKKSGQAAEYFLASPPLSSQWPHRQSRCQSSSPPPQILTNKPPMIDTEKTQTQDQQDSVHLPSTSTAEIYQSHIASMIQDAVAGYNSTLVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLT6</accession>
    <name>E3KLT6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_11454</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178294">
        <property type="protein sequence ID" value="EFP85285.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329704.2">
        <property type="nucleotide sequence ID" value="XM_003329656.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLT6"/>
    <dbReference type="GeneID" id="10527275"/>
    <dbReference type="KEGG" id="pgr:PGTG_11454"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11454"/>
    <dbReference type="HOGENOM" id="CLU_1587329_0_0_1"/>
    <dbReference type="InParanoid" id="E3KLT6"/>
    <dbReference type="OrthoDB" id="10459780at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP85285.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="168" mass="18476" checksum="369A8FCA14CF491C" modified="2012-04-18" version="2">MGMTNSAATLTQPTKETLNSQIRKLVRTPQSHVFNIVVDSFWIISAPPAICMIAHRKIIMQTASTSTGPDRLACSGSEPTPLTLTQHICRWLKIKCSDGLARRKCLAENMTSRRVLVLKALLNMADDCTRLVRRPGQPDQSLIAKLSAGSDPRKGSCRFVGFVDRVSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3KP71</accession>
    <name>E3KP71_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6589 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_12052</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178298">
        <property type="protein sequence ID" value="EFP86096.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330515.2">
        <property type="nucleotide sequence ID" value="XM_003330467.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KP71"/>
    <dbReference type="GeneID" id="10540102"/>
    <dbReference type="KEGG" id="pgr:PGTG_12052"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12052"/>
    <dbReference type="eggNOG" id="ENOG502SCRC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009176_0_0_1"/>
    <dbReference type="InParanoid" id="E3KP71"/>
    <dbReference type="OrthoDB" id="2496395at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046496">
        <property type="entry name" value="DUF6589"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20231">
        <property type="entry name" value="DUF6589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6589" evidence="2">
        <location>
            <begin position="361"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="821"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="821"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="836"/>
            <end position="877"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP86096.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="877" mass="98063" checksum="A2A7FE8FB8DDC26D" modified="2012-04-18" version="2">MAITYLRRPSGKKLMTEDKKISAICDLITQLNYTPKSFLQTFLQRKNMKSAVQRRFWGTRIGWPTTLVLLKSIRGVIVKKPSGRQRWEDFILAEATGIVEAQKPPRGAYPKGAYHNTKNLSPYFFSSKSKRQRTEDIKDHMPFLFELLVNKMGHRPGNNQTMKDGNDLSDCDSDSAETLSAASEDDHYMFSGKTLNGPKKKSSIDHGQRARMTASTMCSMAAFVSNRRHNGHQLANSISFLACGVTDRVSKFLQYVGLSSSRQTAHNSLHRLSKQAERRIATKIGRTPPNLPTLAPFLCFDNLDFEQRIHTQSIGHSTHMFHGTWGYVHQINQALLDSVPKADLTLASYKRAMSNISKLEVTPKMFIPKPPEDEHWEFVLKSQIARTILEHIAQPSDSQVSIAIQPPMIDQLPADEPDITMLKLMVASDNSSQGVGEVFEAIVNQSSISMTDFASRLQIIDADLASCTNISSLRTQRVPSRHPEESLSHVLTILGGSHTLWNIAHAIYSKHYGNCSDSRDSGAWRFLQGLGIPCPNVVDKKDFTLMIKNMDKIHTATLVYCIKLVMGTEKENINDQLEQLPSKTINDLIDQTFDRFFSPEAKKASQGSAKLSNLILRLSDFASVVEGNAAMKAGDVGRLMVVWKRWAVISQGIKKLTQYSVQLPRMILLLNEVLPRGLAHIILHSLLIAPSGRQKHFVAKDQHLEQQNYWLKFFFNHGGRGADIDRLKNVYSVNITLLQNIISDLASDAGKVDIAQSHHNRIGITSLNNCLRMCRQNDVCSTVHKLGEYIPAVIDNFYALGIQTLKVKQLKKGKPINNLRPSTIGVSNSHNHLDNVDDDTDVSSAGDDEPEIDENGAGDENTEGDEQSADDGEWQDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPE1</accession>
    <name>E3KPE1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Pectinesterase</fullName>
            <ecNumber evidence="4 11">3.1.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_12122</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in maceration and soft-rotting of plant tissue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>[(1-&gt;4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1-&gt;4)-alpha-D-galacturonosyl](n) + n methanol + n H(+)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:14570"/>
            <dbReference type="Rhea" id="RHEA-COMP:14573"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:140522"/>
            <dbReference type="ChEBI" id="CHEBI:140523"/>
            <dbReference type="EC" id="3.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the pectinesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.11" evidence="4 11"/>
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        <property type="protein sequence ID" value="EFP86166.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="KEGG" id="pgr:PGTG_12122"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12122"/>
    <dbReference type="eggNOG" id="ENOG502QSQ4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="InParanoid" id="E3KPE1"/>
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    <dbReference type="UniPathway" id="UPA00545">
        <property type="reaction ID" value="UER00823"/>
    </dbReference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
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        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
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        <property type="entry name" value="Pectinesterase_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000070">
        <property type="entry name" value="Pectinesterase_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31321">
        <property type="entry name" value="ACYL-COA THIOESTER HYDROLASE YBHC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31321:SF57">
        <property type="entry name" value="PECTINESTERASE 53-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00503">
        <property type="entry name" value="PECTINESTERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0063">Aspartyl esterase</keyword>
    <keyword evidence="11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005127823" description="Pectinesterase" evidence="11">
        <location>
            <begin position="23"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase catalytic" evidence="13">
        <location>
            <begin position="57"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="319"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005184"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023085"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047928"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EFP86166.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="349" mass="38651" checksum="7098953343A54DC2" modified="2012-04-18" version="2">MAPPIAIYILFSLLFISHICVSSYVPSEISQPKRRQENFPSSTIPPPGSLVVRQTNAKQGDYNTISAAVNALKTLKGPQVIFIGPGTYHEQVHIKYKYPLRIQGYSSDPNSLTGNQVTVQVAVSAASHNGENAPSSAIWVQSPRFEMHNVNVINSFGTGTDTQAVALTAQGEKHVYKYCLFTSYQDTLLIKAASSYFYGCRVEGAVDFIFGSGSAWFEMADIVVKPPKHFATITAQRRAQGGNTMFVFNKSKVLPFSQDTQPNSVYFGRPWSPYSAVTFQSCYLSNIINPLGWIPWNESSDPRTQHVTYQEFNNYGPGSPTQKRQMSSMRSEPVKISDVLGSDYASWAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPS2</accession>
    <name>E3KPS2_PUCGT</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
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            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12263"/>
    <dbReference type="HOGENOM" id="CLU_119179_0_0_1"/>
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    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173601" evidence="1">
        <location>
            <begin position="23"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="150" mass="17640" checksum="21872C8BE887694A" modified="2011-01-11" version="1">MPFIKLGRLLLNRLLLAPSSRAPFTIDDRMSSAQFESFRDQTDEFFADLWNLLRNLLDLCNRDGRATIDEISDELDKSLISFDQCMQVLRPRLVPYSPSNQLLPLTTPQPEREKILNDWFSLLINQVHLAAQNFRVEIEIFRKNYADYIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQ00</accession>
    <name>E3KQ00_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_12149</name>
    </gene>
    <organism evidence="3 4">
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        <lineage>
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            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178299">
        <property type="protein sequence ID" value="EFP86193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330612.1">
        <property type="nucleotide sequence ID" value="XM_003330564.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQ00"/>
    <dbReference type="GeneID" id="10540208"/>
    <dbReference type="KEGG" id="pgr:PGTG_12149"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12149"/>
    <dbReference type="HOGENOM" id="CLU_026798_0_0_1"/>
    <dbReference type="InParanoid" id="E3KQ00"/>
    <dbReference type="OrthoDB" id="2503998at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="148"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP86193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="414" mass="47382" checksum="11D01CAFA7B01A15" modified="2011-01-11" version="1">MTNIREAYLAERKRKNCCRRHADKRTSELSLTVDDTDWTTWVEASAPLPKPQAIGDHPKENFTPSDKSRHHKDSIIWCSKRYIPLELYPHILGDEPERPPTPFEIEYCNTKAAQFKYFHQGKIVIHDKDNESKIIAIAEFTRWDDITPGEVEEIGQLTSFLNTAKCFVNAVDSEGRSWGGNMFAIGWRKAMVAFELFGRYRNNAAIAKAPDVYDNLMQQSQKISSMLGRIFKRLANVAFDSNQELMKEYGIPSIGHLSFGRPINDDDCAPNLTFTSNRFFNSPHCDKDDLSEFAFGMFIPVNRTNWSIGGLKSPSRLSGGQFVFPDYRVGIDFSKHDGLVKLVWRSKEVRHCTLYSTNDEMLDQLGISLQINKKTASASRDTHSGAIFNRRAFRDKPRDKCYIGDHETYVKGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>E3KR86</accession>
    <name>E3KR86_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_13193</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178302">
        <property type="protein sequence ID" value="EFP86811.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331230.2">
        <property type="nucleotide sequence ID" value="XM_003331182.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KR86"/>
    <dbReference type="GeneID" id="10541398"/>
    <dbReference type="KEGG" id="pgr:PGTG_13193"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13193"/>
    <dbReference type="eggNOG" id="KOG3178">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005533_0_4_1"/>
    <dbReference type="InParanoid" id="E3KR86"/>
    <dbReference type="OrthoDB" id="2410195at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012967">
        <property type="entry name" value="COMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712:SF2">
        <property type="entry name" value="O-METHYLTRANSFERASE CICE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43712">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_4G14580)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08100">
        <property type="entry name" value="Dimerisation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase dimerisation" evidence="6">
        <location>
            <begin position="72"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="5">
        <location>
            <begin position="208"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="502"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="502"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="513"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="563"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP86811.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="574" mass="63348" checksum="5149C1726AC57D60" modified="2012-04-18" version="2">MSLSSLADLISNSVQTIEKAVAESAKAQHNQSRYARAHPVTPYEVLEAVDLIVAACAELTSKVQDSTGFICKHALAFNVSSAIRVAVEGHIPEILRPAGPTGMDIQEIAQKCDANPLRLERVLRLLVSHHIFTSPKKRWFANNRHSLALDTGKSAEELPRELNSEKYMGPNTLPALITHSTDEMFKSSSHLTECILEPSSSRSFFGDQSPFSHAFETGMDFRTFLQLSYDQEVRLKRFITATECLSRIGASDQGLSGYNWGTIGNGLMVDVGGGVGHVALSLARSYPRLRIVLQDRAEVIVQAQAFWQKHFPQAVSSKRVILEAHNLFDVQNRRGVRVFFVRFVIREWNDETAVKILKNLSEASSSSTKLILVERTVTNWSQESVNDCPLQTWEILKDVKLSQHVQSSFCHDAAHPQILEETGRPLIDLLDSQLMLYGSGRERTLEELVLMLHAANWKVEKISRPGTSSLTQLICKAIIKSEDEMESIGGWEKKAAVVSASSVPPVDPSSAPTISQLPDQATDSAPSDQLESDLPSLTNSTDHEDPGVSLPVTDPQPIDHTQADQSAMDSQPEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KRV2</accession>
    <name>E3KRV2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12768</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178303">
        <property type="protein sequence ID" value="EFP87027.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331446.2">
        <property type="nucleotide sequence ID" value="XM_003331398.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KRV2"/>
    <dbReference type="GeneID" id="10542106"/>
    <dbReference type="KEGG" id="pgr:PGTG_12768"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12768"/>
    <dbReference type="HOGENOM" id="CLU_2607152_0_0_1"/>
    <dbReference type="InParanoid" id="E3KRV2"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87027.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="79" mass="8528" checksum="BB000C5FD170098A" modified="2012-04-18" version="2">MLISSDTESTLSEGEIVDEMSEDDATADSSLADGRPLLDQADPYSYHVVFLASSEESDFDTDDSASDASTCYYGRDNLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>E3KUL5</accession>
    <name>E3KUL5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_13769</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178310">
        <property type="protein sequence ID" value="EFP87965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332384.1">
        <property type="nucleotide sequence ID" value="XM_003332336.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KUL5"/>
    <dbReference type="GeneID" id="10535024"/>
    <dbReference type="KEGG" id="pgr:PGTG_13769"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13769"/>
    <dbReference type="HOGENOM" id="CLU_1644565_0_0_1"/>
    <dbReference type="InParanoid" id="E3KUL5"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP87965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="161" mass="17607" checksum="8CF1B884ADFF982B" modified="2011-01-11" version="1">MDLRRRNCAGGGGQTILRSQVAFSRRRSEDRLSSFSQETSRPDSAVPLSFSSNLFTVSFELELQSSTLCGTGGSYNKLRREVHTTGSTGVFSFSPLRVTSDVGKPHCQWPEANQLPGSMDGVRRSPAGDDTYIPHPELSSRLIEKCAENLTARTFGWCAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3KUQ2</accession>
    <name>E3KUQ2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein bL28m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_13806</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bL28 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178310">
        <property type="protein sequence ID" value="EFP88002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332421.1">
        <property type="nucleotide sequence ID" value="XM_003332373.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KUQ2"/>
    <dbReference type="STRING" id="418459.E3KUQ2"/>
    <dbReference type="GeneID" id="10535061"/>
    <dbReference type="KEGG" id="pgr:PGTG_13806"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13806"/>
    <dbReference type="eggNOG" id="KOG3278">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1090450_0_0_1"/>
    <dbReference type="InParanoid" id="E3KUQ2"/>
    <dbReference type="OMA" id="ACERTSH"/>
    <dbReference type="OrthoDB" id="361870at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.170.40:FF:000003">
        <property type="entry name" value="54S ribosomal protein L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.40">
        <property type="entry name" value="Ribosomal protein L28/L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00373">
        <property type="entry name" value="Ribosomal_bL28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026569">
        <property type="entry name" value="Ribosomal_bL28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034704">
        <property type="entry name" value="Ribosomal_bL28/bL31-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037147">
        <property type="entry name" value="Ribosomal_bL28_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13528">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L28, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13528:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL28M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00830">
        <property type="entry name" value="Ribosomal_L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143800">
        <property type="entry name" value="L28p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="31"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP88002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="255" mass="28869" checksum="3299406F66C16F52" modified="2011-01-11" version="1">MRLPNQLNRWITTSKHHSSSSSLIRRSISTQTAGPTASKPTLEQQQQGDRHSPKLYGVTYVPPLKITRQHTKRGDYGLYDGLKVRAGDNVSKSKHRTKRTWKPNVQRTTLWSEVLGQSLQLKVTSAALKSIDRMGGLDRYVLQMSEQRLGGMGIRIRELVIAAMQERVRLTKAFSRPIPADQAWRIPRWQDASERTSHAAVRTMVPSVDVSRVAVFPRLASPKPDGSIDPMILHDFHVRGKKGSQTSRRSTHPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KUW6</accession>
    <name>E3KUW6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12513</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178311">
        <property type="protein sequence ID" value="EFP88066.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332485.2">
        <property type="nucleotide sequence ID" value="XM_003332437.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KUW6"/>
    <dbReference type="GeneID" id="10535207"/>
    <dbReference type="KEGG" id="pgr:PGTG_12513"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12513"/>
    <dbReference type="HOGENOM" id="CLU_973637_0_0_1"/>
    <dbReference type="InParanoid" id="E3KUW6"/>
    <dbReference type="OrthoDB" id="2500956at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="192"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="199"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88066.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="312" mass="34315" checksum="0E03C1B35A477AA0" modified="2012-04-18" version="2">MNSSTITTPSSRAHLLLSPDTKLSNPENQSKLNRTNLNHNNNLLQETSSSKKRKSLIAGSGSPVIQPVSSLTRADELKGLDSKAFRLLPINASGTMSPTSEEYTSDVYNQAKVLPAFSTRDQSQSVFDSKTPYTNSMAFFKGGLNKAHKFIGRALEGFPDKESEEEVSDESHDKDEDDLNMLYTVMVPLEFSDEEDGETTSSSSSSSSSPFEPDFDKDSSKNHSEGIPGHCFKKQSSSCLSTNNRHLAPQNEPLTNVTPLEGAEMDSNLDPWLIYSWLPHTDSFAHLFATLLATEISSVNIVCHLCDLQEAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KW86</accession>
    <name>E3KW86_PUCGT</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178315">
        <property type="protein sequence ID" value="EFP88561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332980.1">
        <property type="nucleotide sequence ID" value="XM_003332932.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KW86"/>
    <dbReference type="GeneID" id="10541027"/>
    <dbReference type="KEGG" id="pgr:PGTG_14766"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14766"/>
    <dbReference type="HOGENOM" id="CLU_035631_0_0_1"/>
    <dbReference type="InParanoid" id="E3KW86"/>
    <dbReference type="OrthoDB" id="2500224at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="470"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="496" mass="55189" checksum="3797FEDF21B81A6E" modified="2011-01-11" version="1">MVDANTTRATSKKIKDSASSKRSEDISVANALVPSLNASSGDPEGVMPVSKTAGTPSGSSLPSIGVVSNVVAGKKNTPNETAVDDTVFSLLVDTSETRRDRAPAASLQFPSTAKTGNSAASQIIEDKEEDNSPDIAVSSIMSERQQMWSRAITAQNSGDLILAEMLFKAIGRIGSNAEPDPTPRNKVDVFAIPTQSASGAVIYKDTAIAPPSDNHQAAVQNPEKFYLTEGDLVYAIGTVTNHNSVGFAPFLDENIRKLRSPLPLTIFNRKWQQRAMAHHLDRRQRSEDSSSDKEKVGGYKGFAFVQEWTQTYAQWTRNHRAFRKTLHDVYKIIKFSKLLATHKNNCDDITEEFGFMVAFRYDMEVRSNTFSHRITDEDVKNAIPDISQRKESVVEQCYNVCRNFGELEWEENLYAPGMSHSNHDPLTGYLKPSKYGNSYNNNYQNNQMIQNPGAHGNWAHQQHNSYQPYNHNQNRNQNNTYQQNNSNGPNSQSGNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3KWA9</accession>
    <name>E3KWA9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_14789</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178315">
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    <dbReference type="AlphaFoldDB" id="E3KWA9"/>
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    <dbReference type="KEGG" id="pgr:PGTG_14789"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14789"/>
    <dbReference type="HOGENOM" id="CLU_2134799_0_0_1"/>
    <dbReference type="InParanoid" id="E3KWA9"/>
    <dbReference type="OrthoDB" id="10484465at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP88584.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="113" mass="12665" checksum="1BCB9A0151BA83D0" modified="2012-04-18" version="2">MPADMDQLDLGQIQNQSNQIIAQHNNQLNQFEKVQDLENSSHPSQFNEAQLQYDEIHQDFFGSQENEAKNNEIERNIKLISSIPPLNKGTKFGVDGNHARGIQGFGGGAWGPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3KWQ2</accession>
    <name>E3KWQ2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GH26 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_14685</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 26 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178316">
        <property type="protein sequence ID" value="EFP88719.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333138.2">
        <property type="nucleotide sequence ID" value="XM_003333090.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KWQ2"/>
    <dbReference type="GeneID" id="10541509"/>
    <dbReference type="KEGG" id="pgr:PGTG_14685"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14685"/>
    <dbReference type="eggNOG" id="ENOG502S6DF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043977_2_1_1"/>
    <dbReference type="InParanoid" id="E3KWQ2"/>
    <dbReference type="OrthoDB" id="428177at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016985">
        <property type="term" value="F:mannan endo-1,4-beta-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006080">
        <property type="term" value="P:substituted mannan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022790">
        <property type="entry name" value="GH26_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000805">
        <property type="entry name" value="Glyco_hydro_26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40079:SF6">
        <property type="entry name" value="GH26 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40079">
        <property type="entry name" value="MANNAN ENDO-1,4-BETA-MANNOSIDASE E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02156">
        <property type="entry name" value="Glyco_hydro_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51764">
        <property type="entry name" value="GH26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173976" description="GH26 domain-containing protein" evidence="5">
        <location>
            <begin position="30"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="domain" description="GH26" evidence="6">
        <location>
            <begin position="39"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP88719.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="407" mass="45813" checksum="9E93A2A0DD4139B8" modified="2012-04-18" version="2">MVTNTGKTARFWASVALMANLTIVKMTGALSVDVSAILAKGDEINCSLNKDCDPTELSQSSYENYFNTDKSSWNSLGLDPNLLHINEIAFGFVPAFLEKEKPNKPEDINKKLFKPMSIMGDYVSLSSWDPKLKAVDWHIETVKNLEGNPVWCIALMPSEGLDSVNYEMVYRIAAKMKQVNDLGITVWLRFAHEMNGAWYSWGMQPEKFKNKWRLLAKEIKRVTTRTYMLWSPNAMFGDGIDKVRGGYTPYWPGDGYVDICGLSFYHWGKGAVRTNVKPKDKEAITKLYEFAQIYGPNGMGKPIIVAETAACYTTLAGTSQSVGGGDSEVDIKMTWLHLLVDRYMKHTVPGLKAVIWFEVMKVEAAPGTGEMRNEDFRLVVGNSDVSWAVRQLFASYHSKELSKFSEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KY09</accession>
    <name>E3KY09_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_15051</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178320">
        <property type="protein sequence ID" value="EFP89210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333629.1">
        <property type="nucleotide sequence ID" value="XM_003333581.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KY09"/>
    <dbReference type="GeneID" id="10546847"/>
    <dbReference type="KEGG" id="pgr:PGTG_15051"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15051"/>
    <dbReference type="HOGENOM" id="CLU_1496956_0_0_1"/>
    <dbReference type="InParanoid" id="E3KY09"/>
    <dbReference type="OrthoDB" id="10479387at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP89210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="180" mass="20726" checksum="AC3B49D3DD02962F" modified="2011-01-11" version="1">MDAETLQKYYSHPYDPQFPHYELVHHLPQHQVFGLPDPNLHVKSSPDLKDTVYDQKRRPDGAATFASNNPQPETFPLQSEGFGTEKFELVRSWNLSTPEQAFAKLLSLAVYGVFKNRQGFEMTANAETETQISQAIELLNYNLAEEIPLVKKLDMTRKALLEEMKEEIFKNLENFIPNGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZ65</accession>
    <name>E3KZ65_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EKC/KEOPS complex subunit BUD32</fullName>
            <ecNumber evidence="6">2.7.11.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="20 21">Atypical Serine/threonine protein kinase BUD32</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">EKC/KEOPS complex subunit bud32</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="25" type="ORF">PGTG_15739</name>
    </gene>
    <organism evidence="25 26">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="26" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="26">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. BUD32 has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit KAE1. The EKC/KEOPS complex also promotes both telomere uncapping and telomere elongation. The complex is required for efficient recruitment of transcriptional coactivators.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="23">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the EKC/KEOPS complex composed of at least BUD32, CGI121, GON7, KAE1 and PCC1; the whole complex dimerizes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily. BUD32 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="6"/>
    <dbReference type="EMBL" id="DS178323">
        <property type="protein sequence ID" value="EFP89590.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334009.2">
        <property type="nucleotide sequence ID" value="XM_003333961.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZ65"/>
    <dbReference type="FunCoup" id="E3KZ65">
        <property type="interactions" value="264"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KZ65"/>
    <dbReference type="GeneID" id="10547643"/>
    <dbReference type="KEGG" id="pgr:PGTG_15739"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15739"/>
    <dbReference type="eggNOG" id="KOG3087">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063953_0_0_1"/>
    <dbReference type="InParanoid" id="E3KZ65"/>
    <dbReference type="OrthoDB" id="3399at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000408">
        <property type="term" value="C:EKC/KEOPS complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070525">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000201">
        <property type="entry name" value="TP53-regulating kinase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000323">
        <property type="entry name" value="TP53-regulating kinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022495">
        <property type="entry name" value="Bud32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03724">
        <property type="entry name" value="arch_bud32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209:SF0">
        <property type="entry name" value="EKC_KEOPS COMPLEX SUBUNIT TP53RK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12209">
        <property type="entry name" value="NON-SPECIFIC SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06293">
        <property type="entry name" value="Kdo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0158">Chromosome</keyword>
    <keyword evidence="16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15 25" id="KW-0418">Kinase</keyword>
    <keyword evidence="14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="26" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="18" id="KW-0779">Telomere</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <keyword evidence="13" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Protein kinase" evidence="24">
        <location>
            <begin position="31"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="25">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00019973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="ARBA" id="ARBA00030980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="ARBA" id="ARBA00033194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="22">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="23">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="24">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="EMBL" id="EFP89590.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="26">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="273" mass="30914" checksum="FB609C415EEFFCCC" modified="2012-04-18" version="2" fragment="single">MGLCTATYIGISRRDEHLRKDMMLADFAKVLESSTLIKQGAEAKVYTVELVKAAEGRPGITVLLKYRFPKTYRHPSLDSQLTKNRLTFEARSLTRALKTGVRVPVLKGLDLEQGWLMLEWIEGISLREWLQEHQQHEQSQEELLNLLSDVGTQIAKLHSADIIHGDLTTSNMMLRASKSQGPSRSNQEVVMIDFGLSSVTSLVEDKAVDLYVLERAFLSTHSDPNNRSLKHSSPLFEIVLQAYANYLSQESWMAIKTRLANVRMRGRKRSMVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>E3L0Z4</accession>
    <name>E3L0Z4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_16497</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the aldo/keto reductase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178330">
        <property type="protein sequence ID" value="EFP90219.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="DS178330">
        <property type="protein sequence ID" value="EHS64465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334638.2">
        <property type="nucleotide sequence ID" value="XM_003334590.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888953.1">
        <property type="nucleotide sequence ID" value="XM_003888904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L0Z4"/>
    <dbReference type="FunCoup" id="E3L0Z4">
        <property type="interactions" value="281"/>
    </dbReference>
    <dbReference type="GeneID" id="10546361"/>
    <dbReference type="KEGG" id="pgr:PGTG_16497"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16497"/>
    <dbReference type="eggNOG" id="KOG1577">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_0_1_1"/>
    <dbReference type="InParanoid" id="E3L0Z4"/>
    <dbReference type="OrthoDB" id="2498080at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004032">
        <property type="term" value="F:aldose reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19071">
        <property type="entry name" value="AKR_AKR1-5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.100:FF:000015">
        <property type="entry name" value="Oxidoreductase, aldo/keto reductase family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018170">
        <property type="entry name" value="Aldo/ket_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827">
        <property type="entry name" value="2,5-DIKETO-D-GLUCONIC ACID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43827:SF13">
        <property type="entry name" value="ALDO_KETO REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000097">
        <property type="entry name" value="AKR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00069">
        <property type="entry name" value="ALDKETRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00798">
        <property type="entry name" value="ALDOKETO_REDUCTASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00062">
        <property type="entry name" value="ALDOKETO_REDUCTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00063">
        <property type="entry name" value="ALDOKETO_REDUCTASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="6">
        <location>
            <begin position="41"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="72"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Lowers pKa of active site Tyr" evidence="5">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR000097-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP90219.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS64465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="300" mass="33437" checksum="1257B9562FAC8216" modified="2012-04-18" version="2">MAHLAIDSLQSSAPSLMTNFAAPLTIESRVSLNSGHRMPILGFGTWKLERAEEACSEAIKIGYRHLDSARIYRTEADVSNAVQKSTEDRGSMFLTTKVRRNEHGYESCKKALLDSITAPKPEYWDLVLLHDPLSGPEARHEAYRALAEAQQAGKVKSIGVSNFGVDHLEKLQEADVGPTPAVNQLELHPWCQQTSIVEYCRSKGIIVQAYSPLARGQYFSDDTVVKIASKVRKTPAQVLLRWSLQKGFVPLPKSSSPERMKENASIFDFELEDQDMIELDSLNKDDKGSVTWTPCFKGVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3L1C3</accession>
    <name>E3L1C3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178332">
        <property type="protein sequence ID" value="EFP90348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334767.1">
        <property type="nucleotide sequence ID" value="XM_003334719.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L1C3"/>
    <dbReference type="GeneID" id="10530242"/>
    <dbReference type="KEGG" id="pgr:PGTG_16108"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16108"/>
    <dbReference type="HOGENOM" id="CLU_169162_0_0_1"/>
    <dbReference type="InParanoid" id="E3L1C3"/>
    <dbReference type="OrthoDB" id="10361598at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172378" evidence="1">
        <location>
            <begin position="21"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="116" mass="12864" checksum="92D45538D4F59E7F" modified="2011-01-11" version="1">MRAVTVLTVVVCCLIGLGHARPMQQAPHTLRMIKPRTSSVVVDIPPEIYDPLPPAKTRPVKGYDWVLSNEHVLITTQIKRWGKDIMLGLHLRPLAPEAQKLALTYPTALLAGRELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3L1S8</accession>
    <name>E3L1S8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178333">
        <property type="protein sequence ID" value="EFP90503.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334922.2">
        <property type="nucleotide sequence ID" value="XM_003334874.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L1S8"/>
    <dbReference type="GeneID" id="10530400"/>
    <dbReference type="KEGG" id="pgr:PGTG_16090"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16090"/>
    <dbReference type="eggNOG" id="ENOG502S2AH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011407_4_0_1"/>
    <dbReference type="InParanoid" id="E3L1S8"/>
    <dbReference type="OrthoDB" id="2498701at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040521">
        <property type="entry name" value="KDZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18758">
        <property type="entry name" value="KDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90503.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="941" mass="108562" checksum="5736331EAB01070B" modified="2012-04-18" version="2">MTLTVDQPPVSGTLAQRIRRRRNADRIAATRARFYGGQVANRNSVRRQQQHQEGVSDTIMNNNDHFNSTEDDPVDFTVHEDPTDDTDWITIPADDDDDFDRAAFADRERWRQEAKEVNWNTVLEPLHAYYMDLKARTKNWTDRANSYNDHTNCKCAPHLLTHRMVDLVDIHGAQPFTLALNEWLEPRSERLHARGKKHARELRKPFSATVDLYRTLENMTDELVFKALRLDPQEILASENCPACFGPRPPNAEDYPSHTRDKLIVCLDGNFQHRHHTKASRDYRPIETPRIFLPPSEFANMKTTIRDKEIELSPPARYSKMTQQADRCADSHKAADDKRNESTWKGCDDTGLIGCCCRHDSAIHMGNIYKSGEQRCLPIAIINKLLAECEGERQIGILYDIGCSLDKYMRARDLLGEHRSRLSFGTSVFHAYVHNWICQLDYHPRLNNGWGLSDGEGLERLWSYLSPLVSPLRYSTRNHRLASIAHRLKYHNWKGKRDLPAWLKRKFNAALKRRDEARLVLRGLQEIQNPYAASGVTYTKSFFEFQWREQCAFQKQHTEVEQERRSKLVSLYKQEAVVELLRTRLRGPEIFLATEQQVLDLLASINEHSEQLRRQLDELTGEHGMQAVMNDEESKLLLLLWDAKADLFVQAVHIQAENRPITDSKTIGAHLGTKLKEKIFKASQARRPAVKKHIDHFNRCYLNYVTKFPDQTLSDAADYPLTYDNFVAFPLDHRFWNDGLYYHSKAPWAIDADVRAGITAMLNLSRVQEEFQLLAQELCRAIGWGVSYYNRLTQTIDYLTGRVEAFNLDVEPRPDHIDSLSLYKFPRKHKYKVILRELRRRLASHGTLMDEWSEKVLWLWDRCQPAGNQGFILTWTDMMDKINRSNTINSDVLAGVDEEYEETVLEVDLDDGEDAEATLISSLGGDGASAGVNAGHPVDLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3L2X8</accession>
    <name>E3L2X8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dynamin GTPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17102</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178338">
        <property type="protein sequence ID" value="EFP90903.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335322.2">
        <property type="nucleotide sequence ID" value="XM_003335274.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L2X8"/>
    <dbReference type="STRING" id="418459.E3L2X8"/>
    <dbReference type="GeneID" id="10528316"/>
    <dbReference type="KEGG" id="pgr:PGTG_17102"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17102"/>
    <dbReference type="eggNOG" id="KOG0446">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_355703_0_0_1"/>
    <dbReference type="InParanoid" id="E3L2X8"/>
    <dbReference type="OrthoDB" id="5061070at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048312">
        <property type="term" value="P:intracellular distribution of mitochondria"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000266">
        <property type="term" value="P:mitochondrial fission"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF235">
        <property type="entry name" value="DYNAMIN-RELATED PROTEIN DNM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="303"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="639"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="732"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="648"/>
            <end position="663"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90903.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="789" mass="88586" checksum="7E49E181B3FB3892" modified="2012-04-18" version="2">MLSRFREHKETSPEHLSSTDSKIKSLPVKHGDRIRRLMELTRSLKSSVGHQCDIELPRIVILGDPLAQQTSLLECVTGVKIPREFEHDQRAPIEFRMINVMEVDWSCQVKIRYDYTSQNFKQSPEHVEETNFGEVISEPSKVEGTLREAHSYLLREHAATAPRSSNLEAASGTAPSKLILSKRAFCKNVVCLEIRGRAVVDLTLVTLPNFIQSHSNRALQDFVLSDYLETNCYVVLLTHGLDRLQVHPAFGLAQRVDPTGNRIIGLFNCLDAAQVAQSSPTANLTRVIQSGFRAFRSGSVTKAPPYLPSKPGDSLSRQRSNKSIRAPRAAWKVMRWQNPYFSEATFESTFDDSELVIFFGEIFSELLQESIPKLLGITSQIYSLSSPMTTATKKTGDDTNDSSFCHVYTTCFKDINRLVTSTIAGEELGDEIEAIYEGLGYRAFSDLPLFVPVPRQEDDLPAFKQLTELADMSLYPEAYRTTDPKKIDIRSRRPRIYLADVQDHLSHMKSLNHPKLSGIGHAFCQKTPLMDLPISKWKSHAHDALSQVFQLLQDAVNRIIDQRCSKTPSFQPRIRKLVYLRLDDIFSAAKKSLQTVLEFELVPYTQSLRRLLEIRAGLMEKYHLSCHLLNVGKEKGSPKLTKRGGMYQSASGNQAVSLENRGSPSLDKTDPKLKGWDVEVNACAEASAYLDLALSNLLETTSKIIERELLEELETCDPAQFSPALETEVVEMTASSDPDDHQSPKSPGTSLLHRGNLDPPSKVLNLVGEIQNIIQDETEELLLLDPFQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3L349</accession>
    <name>E3L349_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">H(+)-transporting V0 sector ATPase subunit e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_17246</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the V-ATPase e1/e2 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178339">
        <property type="protein sequence ID" value="EFP90974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335393.1">
        <property type="nucleotide sequence ID" value="XM_003335345.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L349"/>
    <dbReference type="FunCoup" id="E3L349">
        <property type="interactions" value="77"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L349"/>
    <dbReference type="GeneID" id="10528389"/>
    <dbReference type="KEGG" id="pgr:PGTG_17246"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17246"/>
    <dbReference type="eggNOG" id="ENOG502S76V">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_170555_1_0_1"/>
    <dbReference type="InParanoid" id="E3L349"/>
    <dbReference type="OMA" id="WAITYLC"/>
    <dbReference type="OrthoDB" id="1508846at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000220">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008389">
        <property type="entry name" value="ATPase_V0-cplx_e1/e2_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12263:SF0">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12263">
        <property type="entry name" value="VACUOLAR ATP SYNTHASE SUBUNIT H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05493">
        <property type="entry name" value="ATP_synt_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="31"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP90974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="71" mass="7904" checksum="DD1CAE13FC337C90" modified="2011-01-11" version="1">MGGWLIFIVLILAVGLSFLGWVSAPKGDDQIVIRTAVIATITCCYLMWAIVYLAQLHPLIQPKRGDLRPEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3L4R6</accession>
    <name>E3L4R6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178348">
        <property type="protein sequence ID" value="EFP91541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335960.1">
        <property type="nucleotide sequence ID" value="XM_003335912.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L4R6"/>
    <dbReference type="GeneID" id="10531478"/>
    <dbReference type="KEGG" id="pgr:PGTG_17595"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17595"/>
    <dbReference type="HOGENOM" id="CLU_082187_0_0_1"/>
    <dbReference type="InParanoid" id="E3L4R6"/>
    <dbReference type="OrthoDB" id="2495090at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174177" evidence="1">
        <location>
            <begin position="24"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="261" mass="29391" checksum="6F4194FEA99AF60B" modified="2011-01-11" version="1">MTVFGCSLAILSLFTIHLHFISSLPHPGNEHGSQVTLKNTTIDAGGSMPTFRKTPLEHGHGPQLFPSGPKVPTRPALPRHFAWDPNLHIDKGNVVVVNVQTGEPMYTISQSPEDHLTTIVSNLRGDQLLSVCLKKTAGRGVKFVYNTPSGVHYKIDPRLAAPDHWYMMMEGHDPDVSYETLKYFRRRSSNTGPVEFHNHNHTQVASFEFVKSLDKTHWKLDTLGALDKFVTLEITQPTNIGDHYFIGMWALVKRRIDRYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3L6Q7</accession>
    <name>E3L6Q7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_18447</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178361">
        <property type="protein sequence ID" value="EFP92232.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336651.2">
        <property type="nucleotide sequence ID" value="XM_003336603.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L6Q7"/>
    <dbReference type="GeneID" id="10537725"/>
    <dbReference type="KEGG" id="pgr:PGTG_18447"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18447"/>
    <dbReference type="HOGENOM" id="CLU_900573_0_0_1"/>
    <dbReference type="InParanoid" id="E3L6Q7"/>
    <dbReference type="OrthoDB" id="8117402at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF174">
        <property type="entry name" value="CABUT, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="159"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="42"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP92232.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="310" mass="36694" checksum="4185EBB5222B1952" modified="2012-04-18" version="2">MSTPFIPARYPNYSQHEYQQRMFENDHGYDGFMPEVHGDRVTDDEDDEEEYEDDEEEEIREEGGEEEEEEEEGDDDDGEDGEEEVDDYDEGDVDDVDDEDVGEEAEEDEETAEDQEPDQDDEPNQLDRADEKLHPAQDLVQFPKRFESSRALINSKYLLGCPSPGCKKTFDQESNLRLHAMVHTCYRPHVCLPCQARHKRMNELKVHFRNIHTALEFDKEKHVRTKDFDELRSEAFINRYGCTRCPKMFETEDHVKTHFLADHPRHQWSPNMCYFKTLKFVNNEIDQWLSLRRDIESVILHAKLARRKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3L713</accession>
    <name>E3L713_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_18012</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178362">
        <property type="protein sequence ID" value="EFP92338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336757.1">
        <property type="nucleotide sequence ID" value="XM_003336709.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L713"/>
    <dbReference type="GeneID" id="10538001"/>
    <dbReference type="KEGG" id="pgr:PGTG_18012"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18012"/>
    <dbReference type="HOGENOM" id="CLU_035447_0_0_1"/>
    <dbReference type="InParanoid" id="E3L713"/>
    <dbReference type="OrthoDB" id="2504543at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174449" evidence="2">
        <location>
            <begin position="25"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="409"/>
            <end position="438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP92338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="438" mass="45879" checksum="1EA6BF73E0863A24" modified="2011-01-11" version="1">MSKLTNLLSFLYVALMAAEIRVNSAPLNLQDVVLTPGLHELRPLRRRGSYKALLARSPQKLKAAEKEIKKAETAKVDTKDKKESANEGGNNSANTVKQAASDLFKATKNVDNDVLIIQNPASTTEDIKKAAADALKNEDAEDFPRQALASVAQDPEAAQGSLAVIRDKGPIVVQAFKDIHKNAEDKTKVQEELSKVTLARLQVVPANFDLMGGIPGASRSLVSKVAIGPSQQAIGKNITDAAQKKVIEEQQKNLASQTKIVDEQMAIIQKEGSKPEEIEQAAKKALVQEAGEEFAREVLASAATDAKAAMAALGEVRDNGPSKVVQGFKLIESNAKDPAAVKKAADLVVEGRKHVVPANLKLIELSGGSATAGASTDAAAGSASATKDANAAAQTKKDAGETAVKAKDATSNTAKDATANAAKDATANAANDKTANKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3L7T2</accession>
    <name>E3L7T2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_18606</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178368">
        <property type="protein sequence ID" value="EFP92607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337026.1">
        <property type="nucleotide sequence ID" value="XM_003336978.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L7T2"/>
    <dbReference type="STRING" id="418459.E3L7T2"/>
    <dbReference type="GeneID" id="10538766"/>
    <dbReference type="KEGG" id="pgr:PGTG_18606"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18606"/>
    <dbReference type="HOGENOM" id="CLU_381363_0_0_1"/>
    <dbReference type="InParanoid" id="E3L7T2"/>
    <dbReference type="OrthoDB" id="2498269at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055334">
        <property type="entry name" value="PEX8-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39214">
        <property type="entry name" value="MICROBODY (PEROXISOME) BIOGENESIS PROTEIN PEROXIN 8 (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39214:SF1">
        <property type="entry name" value="MICROBODY (PEROXISOME) BIOGENESIS PROTEIN PEROXIN 8 (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP92607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="726" mass="82318" checksum="63A154B4FC73BA10" modified="2011-01-11" version="1">MENVDDGVIYIDVMSWLLSPSQDGCKEDDDQDERMLENLCQLIIHIRPSLVDQLIMAILHSPRLWSIGVRGTAHRDVVVDRDKSGDDSMKKKVIEPFLSIFLKLKFACSTKFHRSSLPQHSNLSTFPVLTGPDDAHQNRLQLKPSTTKFIERWRAVVVGGADENSRAGQFSYFDLRQLCLMAALLISIKSFDLTSASLRLQSLIERDCIKFIKRSIHLTQSATSNVQPVTSFDPDSVITWLAVQIIPSITPINLAESETNDINYHLAKSLFKVFENGHLFNHLSTSLDLTPHSVNLRPQSTTDEVLKNISNDVLAQIVLSISLSISRLIEHTTKTSDLKSFSADLTSLADLTEEIHGSWLTATRSYDQVHLPEFKENSISTKENQTDRWLKNIFFSCLLILGGLVNKLAIRGFSNSMAEAILSPAFRILSQVHFITLRFSSDKLSTHAAVFDTLATLLKPHPGLANLILKECQPSLMDPHDVRDPVRISRVVFYFNLIERLTGSVIQEEYLEQTVVPLVWAFINRAQVGFYAHLALLSVLEFCSKDIVMSLANKYFEILIHDISLDQSSVLSFDQQKVAWKNLLKSVSKHSRSKTEELTSKLIESIKMLDPPVRMIRPAVENGPKNDKTINDQSHNHQNDQRKKLYKLLICEIGELNYDETEEETTKFLDTCWDLIRFQEDLVELFEVGLNQQSFKISNSQLSNPGVRWWLQKTKKINSSAASAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>E3L9N4</accession>
    <name>E3L9N4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Protein phosphatase PP2A regulatory subunit B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_19306</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Phosphatase 2A affects a variety of biological processes in the cell such as transcription, cell cycle progression and cellular morphogenesis, and provides an initial identification of critical substrates for this phosphatase. The regulatory subunit may direct the catalytic subunit to distinct, albeit overlapping, subsets of substrates.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the phosphatase 2A regulatory subunit B family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178387">
        <property type="protein sequence ID" value="EFP93259.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337678.2">
        <property type="nucleotide sequence ID" value="XM_003337630.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L9N4"/>
    <dbReference type="FunCoup" id="E3L9N4">
        <property type="interactions" value="362"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L9N4"/>
    <dbReference type="GeneID" id="10543491"/>
    <dbReference type="KEGG" id="pgr:PGTG_19306"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19306"/>
    <dbReference type="HOGENOM" id="CLU_021713_3_3_1"/>
    <dbReference type="InParanoid" id="E3L9N4"/>
    <dbReference type="OrthoDB" id="6274823at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005935">
        <property type="term" value="C:cellular bud neck"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005934">
        <property type="term" value="C:cellular bud tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019888">
        <property type="term" value="F:protein phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000705">
        <property type="term" value="P:achiasmate meiosis I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071475">
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        <location>
            <begin position="352"/>
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        <location>
            <begin position="353"/>
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        <source>
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        <source>
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        <source>
            <dbReference type="EMBL" id="EFP93259.2"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
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    <sequence length="416" mass="46252" checksum="A8BFC7C05DA76752" modified="2012-04-18" version="2">MYWMETRPAGGDKGEVEDITEADIISTVEFDHTGDYLATGDKGGRVVLFERNESGKKGCEYKFYTEFQSHEPEFDYLKSLEIEEKINKIRWCKRQNSAHFLLSTNDKTIKLWKVFEKSLKVVAENNHMPAGFQPQPVPTLKLPRMAFHDTIIAAVPRKVYANAHAYHINSISINSDGETYLSADDLRINLWNLSISDQSFNILDIKPANMEELTEVITAADFHPLSCNLFCYSSSKGTVKLADMRSSALCDTHAKLYEEEEDPSNKSSRKMGATSCHETIFICGSGDINMDKAPLKTISVHDHLKGKLCDLYENDCIFDKFEGTFSGDGKVTTDIVLQADKSAFKAKKIGASSAGGGAGGSHPGSRNAAGQTNVPNPKIQETENIDFGKRIMHASWHPREDTIAIAATNNLFLFSK</sequence>
</entry>
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    <name>E3NXK8_PUCGT</name>
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        <submittedName>
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        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20261</name>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <begin position="1"/>
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            <begin position="20"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP94307.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="141" mass="15999" checksum="6F0C4495B12BB2E9" modified="2012-04-18" version="2">MRNYGLMFWLACLIANASSTLSHFANVDQFGRWLPVTSLTNFKYQYLVNFIAANPQCGTQLGAGTTGIRHQFGNAKASLTWSGVDSQGEYQSVIVENLSNRKISFLLHNPNTKDFYHWDELDPTVMRVNSEDMARLEFLAR</sequence>
</entry>
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    <accession>E3NXM0</accession>
    <name>E3NXM0_PUCGT</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20273</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS989952">
        <property type="protein sequence ID" value="EFP94319.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338738.2">
        <property type="nucleotide sequence ID" value="XM_003338690.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NXM0"/>
    <dbReference type="STRING" id="418459.E3NXM0"/>
    <dbReference type="GeneID" id="10527450"/>
    <dbReference type="KEGG" id="pgr:PGTG_20273"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20273"/>
    <dbReference type="HOGENOM" id="CLU_063486_0_0_1"/>
    <dbReference type="InParanoid" id="E3NXM0"/>
    <dbReference type="OrthoDB" id="2497616at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="repeat" description="ANK" evidence="1">
        <location>
            <begin position="163"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP94319.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="292" mass="33027" checksum="76670F325A2823DB" modified="2012-04-18" version="2" fragment="single">ILQDIHLLSTSEHLPLTCKRLLDIYSRTTTTRYRAQFLWKKYVLKDSEDGSVSKNRSIRDGAAGRERRKKIRWDAILATPACSIEVLQILRDFFHSDLPAPSDPEDLPKIKVSALPVRLFKALSPTPSDPSPAIIRDHADQLDQAFRYIHLLLTEYGTSPDKFGGYPLARSVLSGNLAFVRLLLDHGARPGVKDNLVIMVAIETGDLELLRLLIEPNFIHPKEAQQSSPLFPNSLPPKSHKKLKLEDRVQITDQMLEQAIKRKDAAMAQYFINKGARPTLETIRLIEALQNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPQ3</accession>
    <name>H6QPQ3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="7">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_20806</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="7"/>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EHS64105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890514.1">
        <property type="nucleotide sequence ID" value="XM_003890465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPQ3"/>
    <dbReference type="STRING" id="418459.H6QPQ3"/>
    <dbReference type="GeneID" id="13542934"/>
    <dbReference type="KEGG" id="pgr:PGTG_20806"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20806"/>
    <dbReference type="HOGENOM" id="CLU_011478_1_1_1"/>
    <dbReference type="InParanoid" id="H6QPQ3"/>
    <dbReference type="OrthoDB" id="5409596at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="61"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="290"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="707"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="733"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="716"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="737"/>
            <end position="747"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS64105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="768" mass="86944" checksum="42046490805D45D1" modified="2012-04-18" version="1">MIRPKKGQPKASLSPQKATTGITPLKKLLEKKDDALKTEIATRAKNDYNGQDPKPIQVETVATLARGKSAFLLAGTGFGKSRIAEMYFKTLPPKSKPVVLVLNPLDALGDNQVEEKKGKFTAINLTKLTFNPFVAEQIKSGVYNFVYLSPEIFLNSKLWDSVYFSPAFQERLGLVVVDEAHMIYIWGLVESSSGRNKYSILVRHQDIGIFRPSYGDLARHLQCRNNIPILLLSATCRRIAVDAILRSLKLTKDLIKMIYDELTRKEIRIIRINMSYSLKSNLDLLTAFPSIEQTATEDLVPTLIYSGTRARTFTVLEVLDMARKTPDACRNAKNMFARRYHSSTGAKDKEDCVRDFSEGRFPIISATMALGLGQNWKRVRCVIHMGRGDPANICQMIGRCGRDGKGGLAILFMESNRKGGKNHPTDFTQATQQSDDDRMDALAITPVCLRVALYSMDNKYGYIPVSKDDPNYITEVKREQSLNFPLCRCSNCLDEEGNTLIKNLQNLNKHNFDDALMDCAQFPIIESITKQRTSRSVQKDLLTDNLELAATFRLHLINVFKNFYEDRMGTSGRFTAIELFNEHHANAVITNFPNILKSDDLNTIIGGETIDGQVDLLMDTISKFRDDPMYHKHMDRCRDLQLEAEALKKQKRREYGIRYRANKRAQLLAKRPEQLFDQYTGGSDSVNDQSVLYTGGSDSVNDQSLLESATAGEQSAEERRKLKRREYAARYRANKKARLEAEKESRVRSSSSQRIETIEELEPTERGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRX4</accession>
    <name>H6QRX4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21589</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EHS63458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889743.1">
        <property type="nucleotide sequence ID" value="XM_003889694.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRX4"/>
    <dbReference type="GeneID" id="13543123"/>
    <dbReference type="KEGG" id="pgr:PGTG_21589"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21589"/>
    <dbReference type="HOGENOM" id="CLU_171268_0_0_1"/>
    <dbReference type="InParanoid" id="H6QRX4"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="87" mass="10153" checksum="0627EF4D2CCBF59D" modified="2012-04-18" version="1">MEDLFGQKSKDGAENRGPESSLGNSLQRQYLVDYWLERQLLNGQKIIRMERLEQIWNNSKSDMVKEDDEIREMLGELGPTRRINALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>H6QS59</accession>
    <name>H6QS59_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21703</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EHS63529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889674.1">
        <property type="nucleotide sequence ID" value="XM_003889625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QS59"/>
    <dbReference type="GeneID" id="13542885"/>
    <dbReference type="KEGG" id="pgr:PGTG_21703"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21703"/>
    <dbReference type="HOGENOM" id="CLU_2961899_0_0_1"/>
    <dbReference type="InParanoid" id="H6QS59"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="59" mass="6695" checksum="FB005478514AD2E0" modified="2012-04-18" version="1">MIQKLLVGMEVDIVSRRVKSEDNVTDAVSRGDRSGRNPAFQLPVQIPYDLESRLYQSTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSJ1</accession>
    <name>H6QSJ1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21805</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178294">
        <property type="protein sequence ID" value="EHS63729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889551.1">
        <property type="nucleotide sequence ID" value="XM_003889502.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSJ1"/>
    <dbReference type="STRING" id="418459.H6QSJ1"/>
    <dbReference type="GeneID" id="13541837"/>
    <dbReference type="KEGG" id="pgr:PGTG_21805"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21805"/>
    <dbReference type="HOGENOM" id="CLU_515932_0_0_1"/>
    <dbReference type="InParanoid" id="H6QSJ1"/>
    <dbReference type="OrthoDB" id="3247418at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46579">
        <property type="entry name" value="F5/8 TYPE C DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46579:SF1">
        <property type="entry name" value="F5_8 TYPE C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="528" mass="60533" checksum="D5B98583C25922C9" modified="2012-04-18" version="1">MEQVQRWEGIDSFHKREKIAKLEGARGSILNELPYWQSAEHCGIEVMHNLLLGNRKDHSFNILKTYQAGNDLKKRVELETKWKKKDIQKIYLAYQELKKIQLDLQHTTTDSGEIFAPGLTISLDSKSSNPTVRRYELHRRSPISTSSLGSIYQFEDEPEEPHQTDEVPETEDLEREGITVEMEEGPTDEPQMKKEELRALQNVISQTKIPTWVSRVPHNFGMAGAGSLKAADWLILYTVYYPLAIVPYWVIGDNKQKENITGSKTVTHQDVLRDSLIKLIEIKNILMKHQLNESDITNYSKLIVEYQQTLQTGWPKQDTKANLHLSQHYPVVIKRLGPPIATAAWPQERLNGMLGKIPNNHHMAEIHRTLTNTWNKKSRYYGLENQLNVSAENLCNEDGSKKVQNTMPKTNKKITLDPKVYKQWRRQISQIYPEHEDHGKLGKHLVNIKSVDLFNCKYVAAKDFHLPSPGNSNVEVSHGGDRFFGCISLLFGACYDDNFCGASFSCAYVLIKCLFLMQAWDQNTLSGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTM1</accession>
    <name>H6QTM1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22068</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178317">
        <property type="protein sequence ID" value="EHS64243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889146.1">
        <property type="nucleotide sequence ID" value="XM_003889097.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTM1"/>
    <dbReference type="GeneID" id="13541324"/>
    <dbReference type="KEGG" id="pgr:PGTG_22068"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22068"/>
    <dbReference type="HOGENOM" id="CLU_517847_0_0_1"/>
    <dbReference type="InParanoid" id="H6QTM1"/>
    <dbReference type="OrthoDB" id="2495703at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="520" mass="58755" checksum="F03F30E20AB9517F" modified="2012-04-18" version="1">MPLAPLPPCPATRVPDLASGISSSSTNTLKYPRKPRSILEFPVEILTNIAVELVINHPSQRHRALKTNQLGKLLRIEARKSNLIEFKELLNFGLTCKYLHSICERIYQKQVVLTLPSTKHTLSQPRLQPRRDKSGRAYSTAGYPYSRTIETHDSVARGWSGAQNVNWLFISNQAYYQPKYSGMFSGLISSTARLARSFSNLKFLCLSRLNNETILSCLERGVGKNLQALSITLSEVEDSESSLRGLANRIARLTTISHLEAIQVNDIEPTWVGLINQVNNLREISLHITNFTPEMVQLLDGHKSLRKLEIFGGVRESPMGPEFFQLVHRKFPEIEVFKCGLHFAGKENLYYGEVNQYASALARFPYLRQFVFNHFNSPQLLDYSDRSTTTDAVLDQFRTYSAPYLVKSQSLQELIFIDFSDRTSVSGISAKKIHSNQDKHVIPAAMPTKPDRLAPKGVLKVLNLCKNQHGMLFRGAEKAGSVATGKNTECSNRTTIKMQKIQDVRDTIMVSFEHRWAKIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUQ3</accession>
    <name>H6QUQ3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PGTG_22484</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The aspartyl protease (PR) mediates the proteolytic cleavages of the Gag and Gag-Pol polyproteins after assembly of the VLP.</text>
    </comment>
    <dbReference type="EMBL" id="DS178349">
        <property type="protein sequence ID" value="EHS64765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888756.1">
        <property type="nucleotide sequence ID" value="XM_003888707.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUQ3"/>
    <dbReference type="GeneID" id="13541641"/>
    <dbReference type="KEGG" id="pgr:PGTG_22484"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22484"/>
    <dbReference type="HOGENOM" id="CLU_022200_0_0_1"/>
    <dbReference type="InParanoid" id="H6QUQ3"/>
    <dbReference type="OrthoDB" id="2506726at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003964">
        <property type="term" value="F:RNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025724">
        <property type="entry name" value="GAG-pre-integrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054722">
        <property type="entry name" value="PolX-like_BBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039537">
        <property type="entry name" value="Retrotran_Ty1/copia-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648">
        <property type="entry name" value="TRANSPOSASE, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42648:SF11">
        <property type="entry name" value="TRANSPOSON TY4-P GAG-POL POLYPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13976">
        <property type="entry name" value="gag_pre-integrs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22936">
        <property type="entry name" value="Pol_BBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0229">DNA integration</keyword>
    <keyword evidence="17" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="15" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0695">RNA-directed DNA polymerase</keyword>
    <keyword evidence="15" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-1188">Viral release from host cell</keyword>
    <keyword evidence="16" id="KW-0917">Virion maturation</keyword>
    <keyword evidence="18" id="KW-0862">Zinc</keyword>
    <keyword evidence="18" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="20">
        <location>
            <begin position="387"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="11"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="67"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="EHS64765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="869" mass="96382" checksum="9A33936A2B9D81B1" modified="2012-04-18" version="1">MSRSEQERNVWSRSSSVSVASTNGSESGFDPEDGSRRMGALFDAVLDGSDETQLADTTIKKPSKGKQPLEGRRAETYEEHRPAPSSSNLNAQAAGGHRQSQNIRHHAPHQVQLTVAKREYQEFQAAKVRLEQAKLISQTVVNINNCWKPENVLSADGSNFLQWTRELREIGASHLSEANFFFSRCDNSTFKKIGRLFIFAGVHQDLVPDIQPLGKTVDIYDFLKKKFTANSRAAQMNIWRRLNGLTIKHTEPSTALVTHVRDLYTELKGLNGRLCSDVVFGFLFQTAVMQSAAPFKKEFEQRVENAIQNDPTKSFPKFEAIVHNYNISQKQWVEYSSQASVALGPQMPSVMEATTDSDDFDFDAFLADVPQDNWSEALEFYAATAIKCWQCKSPGHYARDCTQRAGDHQAAKRRGGSAGTQHQASSHRQLATFVGSLYTQPATQSTPGRSPNAPPSHFQTQAKRLADFYRPRYQQQPTASQHQQSHKGGASAQVMEIGEIPDDLDDLDFRAMTLGEDIVSANVIFDTGASHHFSGSHSLLHDFRHLSKPLPLSVATTADQSYITGVGNLKFRGPNGLIIVIRGVLFCEQARSNLISMAALRKSKASVLYDNDRDAFEIFNGAGDHAFSCLLDKSRNRWCLSYPFLRSDGGGPNGDVSVFVSSINLSANNKTAEPTLSSTIPLFDFPVSSCPSVILPSSSNNPEINAAKAKFNNEEFFKEPIAKIDNFQWKPESLSKEEKQLLFWHRVFGHAGLRTLRKLIKEGLGEGLPKSLPHGSIHCPVCAISKSTAINTLSSTKREIKRMEILAVDLIGPFEVEAIDGSKYLLTLRDAASGYCFVKPIKAKSDSNKVIMDTITRLERITTNKVKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>H6QVA0</accession>
    <name>H6QVA0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22671</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178388">
        <property type="protein sequence ID" value="EHS62801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888565.1">
        <property type="nucleotide sequence ID" value="XM_003888516.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QVA0"/>
    <dbReference type="GeneID" id="13540785"/>
    <dbReference type="KEGG" id="pgr:PGTG_22671"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22671"/>
    <dbReference type="HOGENOM" id="CLU_1525913_0_0_1"/>
    <dbReference type="InParanoid" id="H6QVA0"/>
    <dbReference type="OrthoDB" id="2509305at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003605818" evidence="1">
        <location>
            <begin position="27"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="176" mass="19298" checksum="12920A47C24DE395" modified="2012-04-18" version="1">MLHAISALNSLQPASFFLLRTWGLLATNLLLTQQAFQAIKRPCRYKYQQLNDLFPAKTIDTLFIQPFLPLPASASNHYITSSQHLTMKSVPAILAISILMLLTSALIHVAEGHRSCLTASDQAYCIRPMTGVPKIPKVTKQPDGGYPTFSCPTDMMALCCPSIPPDLKQCNAAPNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>H6QVG7</accession>
    <name>H6QVG7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA topoisomerase (ATP-hydrolyzing)</fullName>
            <ecNumber evidence="4">5.6.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_22747</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the type II topoisomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="4"/>
    <dbReference type="EMBL" id="DS178416">
        <property type="protein sequence ID" value="EHS62950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888500.1">
        <property type="nucleotide sequence ID" value="XM_003888451.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QVG7"/>
    <dbReference type="STRING" id="418459.H6QVG7"/>
    <dbReference type="GeneID" id="13540852"/>
    <dbReference type="KEGG" id="pgr:PGTG_22747"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22747"/>
    <dbReference type="HOGENOM" id="CLU_001935_3_0_1"/>
    <dbReference type="InParanoid" id="H6QVG7"/>
    <dbReference type="OrthoDB" id="276498at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000819">
        <property type="term" value="P:sister chromatid segregation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00187">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.40:FF:000003">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.670:FF:000001">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.199.10:FF:000002">
        <property type="entry name" value="DNA topoisomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.670">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.199.10">
        <property type="entry name" value="Topoisomerase II, domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.268.10">
        <property type="entry name" value="Topoisomerase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050634">
        <property type="entry name" value="DNA_Topoisomerase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013760">
        <property type="entry name" value="Topo_IIA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013758">
        <property type="entry name" value="Topo_IIA_A/C_ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013757">
        <property type="entry name" value="Topo_IIA_A_a_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013759">
        <property type="entry name" value="Topo_IIA_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002205">
        <property type="entry name" value="Topo_IIA_dom_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001154">
        <property type="entry name" value="TopoII_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031660">
        <property type="entry name" value="TOPRIM_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169:SF38">
        <property type="entry name" value="DNA TOPOISOMERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10169">
        <property type="entry name" value="DNA TOPOISOMERASE/GYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00521">
        <property type="entry name" value="DNA_topoisoIV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16898">
        <property type="entry name" value="TOPRIM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01158">
        <property type="entry name" value="TOPISMRASEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM00434">
        <property type="entry name" value="TOP4c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56719">
        <property type="entry name" value="Type II DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52040">
        <property type="entry name" value="TOPO_IIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Topo IIA-type catalytic" evidence="13">
        <location>
            <begin position="112"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="482"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="11">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS52040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EHS62950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="506" mass="57970" checksum="76DB7C887C9006DB" modified="2012-04-18" version="1">MPEFEKWKEENDDGRGWTSKYYKGLGTSDAKDAKKYFSRMSSHRIKFAPTQENDRHLIDMAFNKKKADDRKEWLRGFEPGTHLDQNVSQITYTEFINKELILFSMADNIRSIPSMVDGFKPGQRKVLFACFKRKLKAEIKVAQLGGYCSEHSAYHHGEASLTQTIVGLAQNFVGSNNINVLSPNGQFGTRASGGKDAASARYIFTNVEKITRVIFPPADDNVLNYLTDDGTQIEPEWYIPILPMVLVNGSSGIGTGWSSDIPNYNPVDIVNNLRRRLANEPMEPMHPWFRGFTGQITSEAPGKYKVLGEWDQPDEDTIEITELPVRVWNQSYKEQIEAWINSTDKIPALVKDYREYHTETTVHFIISLTERGKDAIKKEGIEKVFKLSANLSITNMVCFDPSGKIKKYSTPEQILDDFYDVRLAYYQKRKEYLVNDLTLMYERLSNQARFVLMIIEKKLVVSNRKKAEIVAELRKLEFGPFPVKAKPKTAGDPDQADEEVLRKRSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>H6QVH1</accession>
    <name>H6QVH1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22753</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178418">
        <property type="protein sequence ID" value="EHS62954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888494.1">
        <property type="nucleotide sequence ID" value="XM_003888445.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QVH1"/>
    <dbReference type="STRING" id="418459.H6QVH1"/>
    <dbReference type="GeneID" id="13540856"/>
    <dbReference type="KEGG" id="pgr:PGTG_22753"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22753"/>
    <dbReference type="HOGENOM" id="CLU_2151829_0_0_1"/>
    <dbReference type="InParanoid" id="H6QVH1"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="112" mass="12008" checksum="4B018F88E5F25E0A" modified="2012-04-18" version="1" fragment="single">MADSAAQGMGNKLANPSLSSSVSSATSQPLPTTHSHQNFSASSSAIPSHPSRPTPGSSSTSTTNTHHHHHPVQLIPNWPTGERIKTHKDLSYHQLANVKDVKLQLEANLFEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>E3JPS7</accession>
    <name>E3JPS7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">Signal peptidase complex catalytic subunit SEC11</fullName>
            <ecNumber evidence="4 13">3.4.21.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_00223</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalytic component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Specifically cleaves N-terminal signal peptides that contain a hydrophobic alpha-helix (h-region) shorter than 18-20 amino acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.</text>
            <dbReference type="EC" id="3.4.21.89"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="12">Component of the signal peptidase complex (SPC) composed of a catalytic subunit SEC11 and three accessory subunits SPC1, SPC2 and SPC3. The complex induces a local thinning of the ER membrane which is used to measure the length of the signal peptide (SP) h-region of protein substrates. This ensures the selectivity of the complex towards h-regions shorter than 18-20 amino acids. SPC associates with the translocon complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the peptidase S26B family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.89" evidence="4 13"/>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307273.1">
        <property type="nucleotide sequence ID" value="XM_003307225.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JPS7"/>
    <dbReference type="FunCoup" id="E3JPS7">
        <property type="interactions" value="297"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JPS7"/>
    <dbReference type="MEROPS" id="S26.010"/>
    <dbReference type="GeneID" id="10528082"/>
    <dbReference type="KEGG" id="pgr:PGTG_00223"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00223"/>
    <dbReference type="eggNOG" id="KOG3342">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_089996_0_1_1"/>
    <dbReference type="InParanoid" id="E3JPS7"/>
    <dbReference type="OMA" id="ILMNEYP"/>
    <dbReference type="OrthoDB" id="10257561at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009003">
        <property type="term" value="F:signal peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001733">
        <property type="entry name" value="Peptidase_S26B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02228">
        <property type="entry name" value="sigpep_I_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806:SF6">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00728">
        <property type="entry name" value="SIGNALPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EFP74267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="194" mass="21646" checksum="4C6B043C010187A0" modified="2011-01-11" version="1">MFEQELATIKRLGIRHILLQGLNFATVICTALMLWKGLAVALNTESPVVVVLSGSMEPGFYRGDLLFLSLPRDRQLKIGEIPVFNVPEGKIPIVHRLIENHDEPASNNKKPKSAPLAAADKLPTRWMLTKGDNNHENDVALYNGLKYLQRSNLIGKVNGYVPHVGYVTIVMNDYPKLKYALLGVLGLTILLHRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQC8</accession>
    <name>E3JQC8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_00613</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74657.2"/>
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    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP74657.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="296" mass="33899" checksum="7451B2B6B3AD0A1C" modified="2012-04-18" version="2">MQSSLSDTVEEILSALDCIFPEQVILPNQMNDQHLKEIKNYRLYGLHSCIEELLSDIGYFCHFCIQLILELGYSTTKAATNGVEWPKQRLLDSMSDCESLFKSANMWLEGSEWETVLLGWALYIDGLDDVLKLVVLQTNPTDNLEEKLGLPRVLERNLPLIRLLIPIVKLSRVFFRRFFKFGSHGQPLPPFTEMCSRQLHSLHSLPVSVADRLNILHTMLTNSSIYGEDFIESFVERVRSLLGLFQSSFLDLILGIIPPDNDNYKACFFTWNTQLIIATQNIIELALSYSDNPTYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQH5</accession>
    <name>E3JQH5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="8">AlphaK I8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_00443</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74487.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307493.2">
        <property type="nucleotide sequence ID" value="XM_003307445.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQH5"/>
    <dbReference type="GeneID" id="10528179"/>
    <dbReference type="KEGG" id="pgr:PGTG_00443"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00443"/>
    <dbReference type="eggNOG" id="ENOG502QVA3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020761_2_0_1"/>
    <dbReference type="InParanoid" id="E3JQH5"/>
    <dbReference type="OrthoDB" id="2871286at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031037">
        <property type="term" value="P:myosin II filament disassembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04515">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.200.10">
        <property type="entry name" value="MHCK/EF2 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004166">
        <property type="entry name" value="a-kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051852">
        <property type="entry name" value="Alpha-type_PK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45992:SF2">
        <property type="entry name" value="EUKARYOTIC ELONGATION FACTOR 2 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45992">
        <property type="entry name" value="EUKARYOTIC ELONGATION FACTOR 2 KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02816">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00811">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51158">
        <property type="entry name" value="ALPHA_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Alpha-type protein kinase" evidence="7">
        <location>
            <begin position="387"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="99"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="242"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="250"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="274"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP74487.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="609" mass="67236" checksum="79518C0F6AA45B8F" modified="2012-04-18" version="2">MVRCIKCKKWSAGPHFANWCRQCLLFFGLDPDSDPLFLASQSNSLNSILPPNPPPPVPPQSQAIGSSETAATHTSATISLRSSADVPVCAGTKAHFQAVGKAKRHKTPLPSASPYSKSKKVKSSASTDDSNKKIFINAGALVLRDEKLKKLAAKYSHDIDITNPNLYSQLKQELWDLFSSELIQKSMITTPLPASPLDQITLSHGESRLPNLRTLLAYISNAKKKPIPIDIIYEEPSDINTDISTRMSRSSNTQLSSQATSQSTSRRSLRDSTCQLDTRQLATRQPANLHPLTHEPEQSMINHNMFSTVRRNPAGHSQNYGFNNDTWANGPIAATVPCRGVASLDTQIAHLGQPLGRCLITINPDGWIVAQRLTFKNNDYSSIQLRSENAPPSELIATSTPITICIDKSSPIGRGSMRVAYPVKIKTNIDGDGNFSITNFVAKERFIDQHPLISNHATDSRMYQACGLLLQEFKNVLSINTDPNLSPQIRQKAAAFNMVRHCVVVTGNKDIPSKIYFIEAVLRGHYVKYSSNVNFTITRNQPGMDIENLEIMNAFTHWSYINSHGQSLVCDLQGVGNMVTDPQIIDLDPNRWADGNNAAQGIQMFLQNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRR5</accession>
    <name>E3JRR5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00738</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74782.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307788.2">
        <property type="nucleotide sequence ID" value="XM_003307740.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JRR5"/>
    <dbReference type="STRING" id="418459.E3JRR5"/>
    <dbReference type="GeneID" id="10546640"/>
    <dbReference type="KEGG" id="pgr:PGTG_00738"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00738"/>
    <dbReference type="HOGENOM" id="CLU_063287_0_0_1"/>
    <dbReference type="InParanoid" id="E3JRR5"/>
    <dbReference type="OrthoDB" id="2510598at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="173"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP74782.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="289" mass="31755" checksum="3E7AC792FA560F75" modified="2012-04-18" version="2">MARSIFDRPPTPYPTQAHPQPFATARVHHPMAMIVDHPAPVTTTTGSLPLAARISPAGTTPVINGPPFQLAGRGPPPAYSGPGLARPAPGYRRYNISFNRGGFRGGFNPGSNFRPRGSISPFHHFNQRRQAPSRPRTPVFFVNRASIGAPDVPATAPPSPTTPTSSVEELNLARSSSSAPNSSVASAEDFVLPRPRVPSPTPAGMYDPADYHLLLRHQVEALLDRRADPARFDRYAFTPQHIDDLYESITTAMVNHFTTYPAANAAERESRVRLFRFASRRYRTMTRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRW7</accession>
    <name>E3JRW7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01385</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP74792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319211.1">
        <property type="nucleotide sequence ID" value="XM_003319163.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JRW7"/>
    <dbReference type="GeneID" id="10546906"/>
    <dbReference type="KEGG" id="pgr:PGTG_01385"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01385"/>
    <dbReference type="HOGENOM" id="CLU_018307_2_0_1"/>
    <dbReference type="InParanoid" id="E3JRW7"/>
    <dbReference type="OrthoDB" id="2505542at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP74792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="34554" checksum="1427A767033116DC" modified="2011-01-11" version="1">MLGRYSRVQIFFETKDVANSADRIKILGNLIAETNLQSFYANEASGFLTKSWDDFKVRMFDFALPTNWCSSLQRQVRKLEMSQTKTFLEYSTRARTMQSLFNFDANEASKLGDLQLAQFLVYGLPDALQDRIYKRQLLEVAPFAYGSFEKQAKRFLPGFASTDRNTYTDKDYTDAAGACPGPIDRSRIDIPSTFPTPVKPADYVAPRSWGKPTAAPGKATQAPAGRPSTRAASVAGIADTTPLEAQVSALTLDAAMREDGRWDTYFDDEGCFPSLEPAAVAALEDLDSQLLANEIEKAEQAALADAIAGHPGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3JSN5</accession>
    <name>E3JSN5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01653</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75060.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319479.2">
        <property type="nucleotide sequence ID" value="XM_003319431.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JSN5"/>
    <dbReference type="GeneID" id="10547422"/>
    <dbReference type="KEGG" id="pgr:PGTG_01653"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01653"/>
    <dbReference type="HOGENOM" id="CLU_388892_0_0_1"/>
    <dbReference type="InParanoid" id="E3JSN5"/>
    <dbReference type="OrthoDB" id="2507159at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00084">
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        <location>
            <begin position="832"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75060.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="850" mass="94204" checksum="BB4C1FE422340B6B" modified="2012-04-18" version="2">MPPTSAIRELANGGSMPPEAQHQAWIAASPGLAEGRVVMDARPCKSHHSLTRALADGAFSKAPEHQYLAPAGLLQGEFKTASLIKDCRSRHRFQVVEANSPSPSPTSPTEVPTPATLNQLCNSPQSSHADTSQNRLVLKSLHPKSTHPGPSHLSLSCPTTNTSIMNPHHQRTPNSNLVPRSSEITPLTAGHQFSPQHLPPHNVQNSPHSQQSSQAGQHSHYASLQYSQSPGHYNTLNQPLFHPPSPDVFTPTPITPFEHHRARLQSTPHPTGRSTRDQVVFLPILSSNSSSSSSHQSLNPHLNTRRASHINRSDSSCSYASTPGAPIPHQVLRQRVAPLHPSPLSSSTIQSSTCQPSTLTTPPSRVNQPRPGNQDLTSTSSPVPPPPLAGLHDRISKKRKCSEISEDGGPEAQSEEELLKLTEAQLAIQARKASKRAMSDDDRDFFANFHHRQRKLLIIQAIERGVSMPMVDGYLGKRMVIKKPSGWNRFMQTPKVREVFRGAQKGVKDKSAMPLVSTMWNELPEEERLRYMKPSSKKQHNNLDPSIAATNNENPSGEADDDNEDELQVIMRRSVSFKRASDQVQDFMADWFEQAVHISKTCDCEMVMFAVSRHLGTHSFQFTRSTHGATRFVMGAQILDGTRHYAARFQSYITGYEVAQIAKINGKKQGPPKTPSVSTAVRLSHLITEKTEGVIKKWPWTRTEFRLAKLKYRLVLLPGSKTNRDWLMRPGRELKGPREATLHLDLDKKLIDIVHDPLIPDDVTEADYSSSIVSSDSNTGSSTQSNSPQDKQLSNSFHTSLDENDTAVSDQLSHQVNYDGFESDVSHRSIEESEDEESDYEESDDEDVDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3JT12</accession>
    <name>E3JT12_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinase subunit of RNA polymerase II carboxy-terminal domain kinase I</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01832</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003319610.2"/>
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    <dbReference type="eggNOG" id="ENOG502S8PK">
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    <dbReference type="InParanoid" id="E3JT12"/>
    <dbReference type="OMA" id="AHENRPM"/>
    <dbReference type="OrthoDB" id="2555634at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018800">
        <property type="entry name" value="PRCC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13621">
        <property type="entry name" value="PROLINE-RICH PROTEIN PRCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13621:SF2">
        <property type="entry name" value="PROLINE-RICH PROTEIN PRCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10253">
        <property type="entry name" value="PRCC"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </feature>
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    </feature>
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        <location>
            <begin position="330"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="365" mass="39769" checksum="F33591224A2CBA66" modified="2011-01-11" version="1">MALVDYNSSGDENSEAEEAKTETKTNLPPRKKLKPTKARIIIDLPPPTTSNQPELNQNTQPSSIQPQEVHKGDDLLSKLKRKKPASSKSSGLASLLPPPKRTQPQPQPQPSSSSSSSSSLSFKPKALEKPKHEPTTTAQDQAPSAVQSEPIQPTSSTTGTLNLFGLPPTSSSDSSASTKRVPVETLEPQIPLTVSSAPQVAAEVPPPPSLMDPYPGYWQRKDGSWCERDPSDPLWKAFYLQHYSQPTLETTTTTGSSSSSSDKHSKLPKDFFKDAPNSLVQFDAKQVAQTAWENKPKIIDPREEARQEQQASAATKPSKHISSMARGRHQLSSLLTDAQANRAELEDRISRGKFNRKAGGAKYGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUH1</accession>
    <name>E3JUH1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01027</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75696.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320115.2">
        <property type="nucleotide sequence ID" value="XM_003320067.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JUH1"/>
    <dbReference type="STRING" id="418459.E3JUH1"/>
    <dbReference type="GeneID" id="10543576"/>
    <dbReference type="KEGG" id="pgr:PGTG_01027"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01027"/>
    <dbReference type="HOGENOM" id="CLU_593302_0_0_1"/>
    <dbReference type="InParanoid" id="E3JUH1"/>
    <dbReference type="OrthoDB" id="1700726at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75696.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="550" mass="60072" checksum="DAEEEEF7916D2BBA" modified="2012-04-18" version="2">MEDQAVLIGFDQLTSTLIVLFILSYLIPSIINPDQSWLIHPIILAHQARFSPTRKQSETPIITNSGATPHSLPVTPDRSVKSLYDLLLKAEGKLQQIDLHSILPQDVRLNRLITNVRNQLWSQLPSKKNEEHPKILILCEDPYLKLVFTLSAATLPITTIVGSSKPTGTISIDSKAALEPYLDSLSLVISDLSSKDLVQELLGPKLAGDCLVLSTAELDCNAWFLADDQRGDSAPHDPALVSQARLRIIHQQQRSSSSTCSNDSTKKEGEGVEVYEYTPENILAGITGLLGIFPVTGKMTSKDRIFLEFNNSDGDDDLWGSEISVALAGLYSGADVYFFNGFKLLSEYKPTILMIKAMNAQILAEEIIQLSHLSLLKKFTIGRRLSQVYNGNLGSPLVETGEWVGPQLRAILTKDPISQSTATLIRAGFGVSMQRIYAHPISTGPILVSQQYDFQVPQSTTRATKSMMGCGPPAANVECKIVGVAEPSLVNHDAWKGKLVVRGPSIGTRIVPSRDPTSQQEDCFFPVAEIAQVLPNGTFLLFPPSSTGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3JV63</accession>
    <name>E3JV63_PUCGT</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01269</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75938.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320357.2">
        <property type="nucleotide sequence ID" value="XM_003320309.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JV63"/>
    <dbReference type="GeneID" id="10533016"/>
    <dbReference type="KEGG" id="pgr:PGTG_01269"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01269"/>
    <dbReference type="HOGENOM" id="CLU_634821_0_0_1"/>
    <dbReference type="InParanoid" id="E3JV63"/>
    <dbReference type="OrthoDB" id="2498109at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="286"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="404"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="404"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75938.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="460" mass="50967" checksum="DD6770D695975F02" modified="2012-04-18" version="2">MTPSLVEPAISKHQESYQYLCLTTHAGPQDEPHSDAASVQTQHLSPDNSFSGERANEPPVQEIPADSDRQHVCRARRLPLELRFTPVNSPTDNSGPVKPSRSENAITPQLSRKDHRHHARHCRLIGFTPLAEDIPASSSDENSTQFLGPRKVKAKEVAPCALLYKRSDVFVSSEMKSQNILAYSENHYRNLRFDDEISISEIEYAPQPTNQVDKRFQSPTDSKDVESFTVKESDMKVHLEPLCIDREPIVHAFPKDRLTSIPLTSESEPGSHFSYDHFHLFGDETESPLTTGSGAEHSLTWESPYKPTGASSDLEQSISSINSSGIQSSLWDTTLSSLTRATSPVSSIYSSVMPIYDHQLVKTSSEERCPQSECKLVGFPHLTMNTGNESAKSSMAQEAFDWEEDHGSLADDENDSDCSSIQLESDDDRELSGYTATCFQMIPSASLAGFSKKGSVVLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3JVM7</accession>
    <name>E3JVM7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02543</name>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320521.1">
        <property type="nucleotide sequence ID" value="XM_003320473.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JVM7"/>
    <dbReference type="GeneID" id="10533506"/>
    <dbReference type="KEGG" id="pgr:PGTG_02543"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02543"/>
    <dbReference type="HOGENOM" id="CLU_888862_0_0_1"/>
    <dbReference type="InParanoid" id="E3JVM7"/>
    <dbReference type="OMA" id="FAMFEDF"/>
    <dbReference type="OrthoDB" id="2499319at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="254"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="326" mass="36091" checksum="692A5B90152FA704" modified="2011-01-11" version="1">MLNPSQTPSPKPKSRFFSNLRRSLRSNSQLTSVPESLFDPVPKTLFSTATESPSHADTSSSYATSSSSSNPSSPASNTPSRQLTPPQRRHASSFSKITFSAAEFLEDRYIYAQSPEKLCLSHELMSDESLPEDDLERVPFFAIPTTRKVSYGTQDMQSQEQTSNAHTKPIPAKKPLIKFYEDYGDLLPEFNYEDPCSSTTAQRKPMPASNINQPEFAPRLSCSSTPSPGGLDRHNDPYCSESYSSDLGHDDSPTLGYYSEFPTTSKHSSPKAVNWTSEKTQASRSRIIDPGNLLIPCDFDFLSSILDTPPPIPQETFTDDLVGMAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3JW00</accession>
    <name>E3JW00_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_02666</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76225.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320644.2">
        <property type="nucleotide sequence ID" value="XM_003320596.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JW00"/>
    <dbReference type="STRING" id="418459.E3JW00"/>
    <dbReference type="GeneID" id="10533955"/>
    <dbReference type="KEGG" id="pgr:PGTG_02666"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02666"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_900586_0_0_1"/>
    <dbReference type="InParanoid" id="E3JW00"/>
    <dbReference type="OrthoDB" id="2498342at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000064">
        <property type="entry name" value="Early growth response protein 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF120">
        <property type="entry name" value="KRUPPEL-LIKE FACTOR 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="247"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="278"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="83"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="195"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="46"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="94"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="135"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="195"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP76225.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="335" mass="38060" checksum="D64B18786D2E1C21" modified="2012-04-18" version="2">MMNRPNLPPLRLALAQCDDRDHSNRDTPHRSGYSTRLHSDLSFYSLASSSTPPTSSSSSHIPSPKRDLLPSFMIENLGFSSLKPSINHSERRSSGSAYRTSGSRQHNYIELKDNLGHDREVQPERKSTRGISPWEKSNPQFFRSYRTTQSENPGVPHDKPMSPYHSECHTVSGGIPFNHYERPELEVNKTSKAINSQLPPNLSDQSANLEEPQYGCADPGGRPTQEGPEISPSYRVQPTETASGTRYFCPVWGCGKSFTRNFNLTQHISAIHKDERRFQCTYCMALFYRRNDLTRHERAHTGVRPYQCECGQNFTRTDLLARHKHSGNCAYKKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3JW91</accession>
    <name>E3JW91_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02757</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76316.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320735.2">
        <property type="nucleotide sequence ID" value="XM_003320687.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JW91"/>
    <dbReference type="STRING" id="418459.E3JW91"/>
    <dbReference type="GeneID" id="10534208"/>
    <dbReference type="KEGG" id="pgr:PGTG_02757"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02757"/>
    <dbReference type="HOGENOM" id="CLU_002101_4_1_1"/>
    <dbReference type="InParanoid" id="E3JW91"/>
    <dbReference type="OrthoDB" id="3039677at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46579">
        <property type="entry name" value="F5/8 TYPE C DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46579:SF1">
        <property type="entry name" value="F5_8 TYPE C DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76316.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="444" mass="50850" checksum="4E8D340B5119CF67" modified="2012-04-18" version="2">MTTITHLMRPLIDELVGLTTPFNLETHTHPNGRTVQIKLLPLIGDLGATHKVAGYASHSANYFCSWCKVHKDECHKLEFGQPRTGSEVQKISKSWLDNKTIKGREEILRVNGVRYSELNRLTYCDPVKHVALGAMHNWMEGVLMHHFRERWGFQTLSVKEKRNRGVDTDNHASRKRPRLETGNDDTVSLDQDDEDLEDSDFELDQRSGGLFTELDMNLFRSALPEVVLPTVVGCIPSQLGKAKCGKLKASQWYVLFVYVIPLIVADIFVTDIDKINAKSNQALIVDNIACLIQCTHIVNSRHVKEVHGKRFEDSYQKYNETSKKIFENLAVHPNHHYALHIPKQLKLWGTLGGVAEFTGERMIGKLQNIETNHRMGELEGTMMKRICQIQRLEARDEFTNILDDLKDDQKPETRGRAIMVNKEIYHVSYGSWVRKLDSTECSKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXC6</accession>
    <name>E3JXC6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycoside hydrolase 131 catalytic N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_02162</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321120.1">
        <property type="nucleotide sequence ID" value="XM_003321072.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXC6"/>
    <dbReference type="GeneID" id="10528721"/>
    <dbReference type="KEGG" id="pgr:PGTG_02162"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02162"/>
    <dbReference type="eggNOG" id="ENOG502SKJS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063723_2_0_1"/>
    <dbReference type="InParanoid" id="E3JXC6"/>
    <dbReference type="OMA" id="HGENQTW"/>
    <dbReference type="OrthoDB" id="5283326at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041524">
        <property type="entry name" value="GH131_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34612">
        <property type="entry name" value="GH131_N DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34612:SF2">
        <property type="entry name" value="GLYCOSIDE HYDROLASE 131 CATALYTIC N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18271">
        <property type="entry name" value="GH131_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171601" description="Glycoside hydrolase 131 catalytic N-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase 131 catalytic N-terminal" evidence="2">
        <location>
            <begin position="55"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP76701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="340" mass="37163" checksum="7CB8442D3653262A" modified="2011-01-11" version="1">MLVYKMQQALLMAIFLFASVSLAVGPVPGMGSVVEERNGANTNSGAAGGAPKRIFDFTLTPNMKTADLDNPQSALSKLIAYIVKSAAKASTYVSILHPSNAASQIQLAIRDDSIFIPGGNPANAQHGFRRTDVNPAIDKATTLTGITTWYQTIRLDSKAPLVLTHGYLFASIEVPSGDHIFDIFGGSNFDPQNTDHKPTSNSQTIRVRDFNTKTLLSLPLKYDQDYNFAVTVDWTQNTLTVYSSIGNQPLQKAGGPFPNDPKAMSPEFKQKGEYHIQLIKFPLADAKVPVAKRLDTPHFGFQEPIKQEHVTFSNVYATAGKEIEQPKFSGSKSKKIKRSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXS3</accession>
    <name>E3JXS3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNI-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_02309</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321267.1">
        <property type="nucleotide sequence ID" value="XM_003321219.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXS3"/>
    <dbReference type="STRING" id="418459.E3JXS3"/>
    <dbReference type="GeneID" id="10528953"/>
    <dbReference type="KEGG" id="pgr:PGTG_02309"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02309"/>
    <dbReference type="eggNOG" id="ENOG502SAZM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_525932_0_0_1"/>
    <dbReference type="InParanoid" id="E3JXS3"/>
    <dbReference type="OMA" id="HIESMDA"/>
    <dbReference type="OrthoDB" id="120976at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027038">
        <property type="entry name" value="RanGap"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113:SF15">
        <property type="entry name" value="NACHT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24113">
        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00368">
        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP76848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="506" mass="55551" checksum="4B044B9B91F9EE90" modified="2011-01-11" version="1">MSSLFPLLRSLLISPLKPTSTTTNTIQQIIQDRDRGGFTTPDRQQDHLSFHDTGLRGTQGALIVLEALATNPFASRLTLSHNILGDQGVRQLASRLRFLKKRRATPIHELNLASNALTDAALIELCRTSDGLLELYLSNNHISLNCSPSPFLAGLGNLTLLSLTSNPIDCLALSDLLRNPKLALLQLNTLHLSACALDLPVAVSLALWLEDRSRSGTLEWLAVNGNNWGTAGCERIVWALARRGGNSNLLRVEMLACDAPPSEAEPTEGRDQMEIVKLMGFRDSNDLEDCHLTVQLEGGWKKLLEKCEARNQALRLATRRAALGLVSTARPILLGTPRPPASAEAETMEASKARVFRWDKLPEELKLHIWRWVAILSAFPGLQSLELPDHHQQQGPSLSAADQVHSRAATATSRAAAVDPFILPDPLTPSQLFSLINYAQDRESLQAEIALREEAIFSHLIGSKSSSSSSVHRQLVDNLRRDADIDRQGRAFILSSCGCLRFQGDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZH5</accession>
    <name>E3JZH5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03406</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321869.1">
        <property type="nucleotide sequence ID" value="XM_003321821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZH5"/>
    <dbReference type="GeneID" id="10538328"/>
    <dbReference type="KEGG" id="pgr:PGTG_03406"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03406"/>
    <dbReference type="HOGENOM" id="CLU_032239_0_0_1"/>
    <dbReference type="InParanoid" id="E3JZH5"/>
    <dbReference type="OrthoDB" id="10330747at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="307" mass="34270" checksum="FF17B763070A86DF" modified="2011-01-11" version="1">MSSRYSTPDYTLDYDVRSEPSVEYNYVQNGIRYYPDRDMWSPVWEPEPSQTTRPSPNDMVVDEPVVEDVKPNISVAGGSDGQSVANPAQATDAHQATNTGIMPKIMIPKGLEIVETPKYYFPLKAKWHSVYDPQGRYDLSSIPTAVPRMNIHNDPHPFVCVAWNVHDPELPPGLPVPFLKTTAPIDLFSASSSPAPSSQSSSAPSTLSDASSTASSAYPPISAYIGLNHQGVQRLLLDDNNPPSAVCIYTEKDVMRIFEGIAPDYVHMCSEYPPSSLAESDRRVMFFENMQWHFRNKRSTYLSTRNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZT9</accession>
    <name>E3JZT9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Haloacid dehalogenase-like hydrolase domain-containing protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_03520</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77564.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321983.2">
        <property type="nucleotide sequence ID" value="XM_003321935.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZT9"/>
    <dbReference type="FunCoup" id="E3JZT9">
        <property type="interactions" value="196"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JZT9"/>
    <dbReference type="GeneID" id="10538603"/>
    <dbReference type="KEGG" id="pgr:PGTG_03520"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03520"/>
    <dbReference type="eggNOG" id="KOG3085">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045011_8_0_1"/>
    <dbReference type="InParanoid" id="E3JZT9"/>
    <dbReference type="OrthoDB" id="444127at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.720">
        <property type="entry name" value="Haloacid dehalogenase-like hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051828">
        <property type="entry name" value="HAD-like_hydrolase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044924">
        <property type="entry name" value="HAD-SF_hydro_IA_REG-2-like_cap"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46191">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46191:SF2">
        <property type="entry name" value="HALOACID DEHALOGENASE-LIKE HYDROLASE DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP77564.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="292" mass="33681" checksum="39BE13585A74A680" modified="2012-04-18" version="2">MAKMSGLCHLSSRIQRNGTHPAHVFLDLFGTLIYPALPIHIRYNQIAHANGFGDFKLSAKNFKTAFKKTNKEFPNYGKNAWVTDNQEKHVRMLPKDWWLKVVKGTFEDSLNAQESLPRPKLSEIEKISELIYEEFLTNEDEKIYKNYEDTLDFINYLTTEKIGFSILTNSDPKILNALPPSLKELLFSKDQINSQFQAFTSWDIGFSKPDQRIWDSAISQLNLNKSKDRLIHVGDDFQEDFLGAKSAGIGSIWLDRQDNHQKISKPINNEIANHQFKAHERIHSLNELIGFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3K094</accession>
    <name>E3K094_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Transcription initiation factor TFIID subunit 1 histone acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_03675</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322138.1">
        <property type="nucleotide sequence ID" value="XM_003322090.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K094"/>
    <dbReference type="FunCoup" id="E3K094">
        <property type="interactions" value="44"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K094"/>
    <dbReference type="GeneID" id="10539091"/>
    <dbReference type="KEGG" id="pgr:PGTG_03675"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03675"/>
    <dbReference type="eggNOG" id="KOG0008">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000572_0_0_1"/>
    <dbReference type="InParanoid" id="E3K094"/>
    <dbReference type="OMA" id="GEKTWQQ"/>
    <dbReference type="OrthoDB" id="5752at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040240">
        <property type="entry name" value="TAF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022591">
        <property type="entry name" value="TAF1_HAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041670">
        <property type="entry name" value="Znf-CCHC_6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13900">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13900:SF0">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12157">
        <property type="entry name" value="DUF3591"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15288">
        <property type="entry name" value="zf-CCHC_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Transcription initiation factor TFIID subunit 1 histone acetyltransferase" evidence="6">
        <location>
            <begin position="407"/>
            <end position="863"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc knuckle" evidence="7">
        <location>
            <begin position="1058"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="20"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="881"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1023"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1107"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="22"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="47"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="91"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="120"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF12157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF15288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP77719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1131" mass="127082" checksum="8BD4C21CB7774443" modified="2011-01-11" version="1">MDPLGESLRAQLGLSSANIGYGREDLYNGKFADDDTGIPADYGENASQDRRGRLLSDSSLKKDWEDEVDKEFQEELERRPSTIGKVRGETDDYDEEEDIDEDESLAADSPQVFRPKKHSQQLQLSHRYSSSGSTPSSAFQIPSSPTVVGLEKNEPEPANVHDLFPTFVEGGILQFTDLFATVPRKRQKVTGGYVDVNLPETLPPAMSTSYLLEKPTLNLSQRSPDQSKLPRMLQESYQELLATLPPVKEESNFQSKIPSRPLVEETSLEQLLAQQASKCATPWRIPHDDRGWQALSMQDWESDIIWKPQPSPNPLSQPIDTLTRLSRPRNPEVEEGDWLDCIIYDAKNRPWKDFTTLHVVEEDARSLSNNQAQGVEIEKEVVKSKAPIARTSSTLNRDSKQQTLDPFNLSNDRFYEVSKEHRRRVRQTFGNLEVQHSYPAMKLQLPLYKTRLTKHEARSFHRPALQFPVNVPIKFSKVKVAKKKKDKAGRKIRNTAPAAELLKNTTDLTLKDTGPFMLFEYSEEAPPVISNVGMGSILVNYYRKQNELDDHIPNDDIGEPFVLDVADESPFMKFGSIRPGETVPTLYNNLVRAPLFKHTPSSNEFLVIRNTTETETNYYIRPIDHLYVVGQTYPVVEVPGPHSRKTTTQLKHRLQTIAFKLCSKSEDGRIRIGKVMKYFNDQNEMQMRQRLKEFMEYTRKGHNQGFWTIRPGLKVPDKEEIFKLVTPEGVCLAESMQVGQRQLLDAGYGKDADIEGDGDDQSKLDIEQQLAPWSTTKNFLAATSNKAMLRLYGEGDPSGRGEAFSFIRVSMKEMFLRDGETLEERQAELASRPKSSHRLHVGEQQDVYKQEIERIWQAQRLALSDPNPPQLTHEDEIRARGGRGAIGNTPATGIDTPLPHPSAAGATPGDWNTSRRSRSPSSFSRQSSPERDDGMSVGSRNHVVGANKALKIRRLIDGKWQTEVVRDPTVVNAYLRQRRLIDEQESSAEALMPSDDPEKNRLAKRRIEEALAKLKRNQERRLARKNAKENGAAFPKPGSHNKIDVALSTPVSGSQPVRKCGNCGQVGHMKTNRKCPRWAEFNSSAAGVFGAVPVSTLGNYTTQGAPVAGTSEHGGSVAGTPNPAAYHGMEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3K0G0</accession>
    <name>E3K0G0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_03741</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322204.1">
        <property type="nucleotide sequence ID" value="XM_003322156.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K0G0"/>
    <dbReference type="FunCoup" id="E3K0G0">
        <property type="interactions" value="138"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K0G0"/>
    <dbReference type="GeneID" id="10540607"/>
    <dbReference type="KEGG" id="pgr:PGTG_03741"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03741"/>
    <dbReference type="eggNOG" id="KOG1208">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_44_6_1"/>
    <dbReference type="InParanoid" id="E3K0G0"/>
    <dbReference type="OMA" id="FTWFRYA"/>
    <dbReference type="OrthoDB" id="2499330at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05327">
        <property type="entry name" value="retinol-DH_like_SDR_c_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320">
        <property type="entry name" value="RETINOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24320:SF282">
        <property type="entry name" value="WW DOMAIN-CONTAINING OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173429" evidence="5">
        <location>
            <begin position="24"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP77785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="338" mass="37096" checksum="060E58A04689F344" modified="2011-01-11" version="1">MDAAVFLGLLHALFDGFLRFICANIPPFRRRWSVKEMPELSGRVAIVTGGNTGIGFATCLELARRGAKVYLASRTESRAKAAIQKIKQLVPDAQVDFIPFDLRILSSAKTAAEEFKGKESRLDILINNAGIMAAPYELSPDGIEVQACNATGHFALTSHLLPILKRTASSISDSHVRIVNVASVAHLFGTDPDFSSLEGMNRPNDWPMSRYDYSKLSNVLFTNELQKRLSDTSIYCLSVHPGLVASQITRSVISESLTDFLAKIGSYIISSPHEGALTQLYAATSPEIESKGLRAAYLVPYGQVGRKSLIAEDRGGKLGRQFWDLCERLMQAAEDKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3K0Y5</accession>
    <name>E3K0Y5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Glutamate--cysteine ligase</fullName>
            <ecNumber evidence="3 10">6.3.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 10">Gamma-ECS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8 10">Gamma-glutamylcysteine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_03916</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-cysteine + L-glutamate + ATP = gamma-L-glutamyl-L-cysteine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13285"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58173"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glutamate--cysteine ligase type 3 family.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.2" evidence="3 10"/>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP77960.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322379.2">
        <property type="nucleotide sequence ID" value="XM_003322331.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K0Y5"/>
    <dbReference type="FunCoup" id="E3K0Y5">
        <property type="interactions" value="217"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K0Y5"/>
    <dbReference type="GeneID" id="10541108"/>
    <dbReference type="KEGG" id="pgr:PGTG_03916"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03916"/>
    <dbReference type="eggNOG" id="KOG3754">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010467_0_0_1"/>
    <dbReference type="InParanoid" id="E3K0Y5"/>
    <dbReference type="OrthoDB" id="7939818at2759"/>
    <dbReference type="UniPathway" id="UPA00142">
        <property type="reaction ID" value="UER00209"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017109">
        <property type="term" value="C:glutamate-cysteine ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004357">
        <property type="term" value="F:glutamate-cysteine ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006750">
        <property type="term" value="P:glutathione biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.50:FF:000007">
        <property type="entry name" value="Glutamate--cysteine ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.50:FF:000002">
        <property type="entry name" value="Glutamate--cysteine ligase catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.960">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.50">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004308">
        <property type="entry name" value="GCS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11164">
        <property type="entry name" value="GLUTAMATE CYSTEINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11164:SF0">
        <property type="entry name" value="GLUTAMATE--CYSTEINE LIGASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03074">
        <property type="entry name" value="GCS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0317">Glutathione biosynthesis</keyword>
    <keyword evidence="4 10 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU367135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP77960.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="668" mass="75672" checksum="7B358CAB808C4630" modified="2012-04-18" version="2">MGLLALGTPFDWLEAKQYADHVRQQGIQQFLRLWRNLQGRSDDLPLWGDEIEYFLVSLDHTTRQANLALRQEEVLESLQQPSTAPLPPQIDVRPTFHQEYGRYMLESTPGAPYISTLEDCLRVEFNMRYRRELIRAKLAENERLVTLTSFPRLGAPQGFTVPYEAPCGSVAQSLYLPDDVINQHIRFPTLTRNIRKRRGSKVAIFVPVFPDLNTPKPFRDPTVPAVPPETSVAPGALPSPLANGDRFPNGTEKIAIPAIPDNSIYLDAMGFGMGCSCLQTTYQAQSVTQARYLYDALATVAPIMMALSAAAPIFKGFLSDVDCRWDVISGAVDDRTEPERSPENLNQKYIPKSRYDSISLYIADDARHRPEYDDVLAPVDKSIESQLFQNGLDHIMARHIAHLFIRDPLVIFSETLEPRNDDSMDHFENLQSTNWQSVRFKPPPSASSSSDIGWRVEFRTMEVQITDFENAAYSIFVVLLIRAILMFDVNFYIPLSKVDENMRRAQQRDAARSSLFYFRTQIQTKPNTTATSSLQQQGMTPSVPVDGIKPEFEEMTMDEIIHGKQSSQYPGLMHLVLSYVDKTDADDLVKENIKKYLDFISARATGRLMTTAAYIRKFVQEHPAYQQDSVVSSEINYDLICRINQIEAGKHRAEELVGPGYSSTLPHT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3K1W6</accession>
    <name>E3K1W6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADP-dependent oxidoreductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_04291</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the shaker potassium channel beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78335.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322754.2">
        <property type="nucleotide sequence ID" value="XM_003322706.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K1W6"/>
    <dbReference type="FunCoup" id="E3K1W6">
        <property type="interactions" value="16"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K1W6"/>
    <dbReference type="GeneID" id="10542289"/>
    <dbReference type="KEGG" id="pgr:PGTG_04291"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04291"/>
    <dbReference type="eggNOG" id="KOG1575">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023205_2_0_1"/>
    <dbReference type="InParanoid" id="E3K1W6"/>
    <dbReference type="OrthoDB" id="1720422at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.100">
        <property type="entry name" value="NADP-dependent oxidoreductase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005399">
        <property type="entry name" value="K_chnl_volt-dep_bsu_KCNAB-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036812">
        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023210">
        <property type="entry name" value="NADP_OxRdtase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150">
        <property type="entry name" value="HYPERKINETIC, ISOFORM M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43150:SF2">
        <property type="entry name" value="HYPERKINETIC, ISOFORM M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00248">
        <property type="entry name" value="Aldo_ket_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01577">
        <property type="entry name" value="KCNABCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51430">
        <property type="entry name" value="NAD(P)-linked oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADP-dependent oxidoreductase" evidence="4">
        <location>
            <begin position="102"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP78335.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="407" mass="45190" checksum="30F83B822C1D655D" modified="2012-04-18" version="2">MFSYNRNTVRQLSLFIMIFDQTTSAMTPVNSLVTEAAATPQVFGEGLLKGLNHGADSRSIMPPPKYQIKDMKFNQLGHWGLRVPIFSYGGALSVGSVATGAAVKELLQIAWDHGCNYFDTAEGYANGKSETELGKALKELGWERSEFMVATKIFFGTGTSQEPNARGLSRKHIIEGLMASLKRLQLRYVDVVHAHRPDPTVPIFETVWAFDYLVRNGHALYWGTSEWSAQQIQEAVTVAGTNLLIPPMVEQPQYSLLHRERFEVEYEPIFKNLGYGSTIWSPLKGGLLTGKYNDGIPAGSRLEVNKEYLKHLVAGLQTEEGRLEIEKVKKLTQLAEDKLGCTVGQLALAWAASNKRVSTVILGATKPEQLRENFGALKIIDKLTPEIKEEIEAIVDNAPTHPSTWGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3K1X5</accession>
    <name>E3K1X5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322763.1">
        <property type="nucleotide sequence ID" value="XM_003322715.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K1X5"/>
    <dbReference type="GeneID" id="10542298"/>
    <dbReference type="KEGG" id="pgr:PGTG_04300"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04300"/>
    <dbReference type="HOGENOM" id="CLU_1107572_0_0_1"/>
    <dbReference type="InParanoid" id="E3K1X5"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="219"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="251" mass="28539" checksum="0F5009C9E4C1FD13" modified="2011-01-11" version="1">MLISNCSRWMGQGSKKRKRPILQKYNLKSCDCSRKGTSRTIAEVVLDRLKDQTRNFSNQLILLNYRPKEEQVRVQSRTKERNCSALSISNAEVPCQARIDKRKPESTVSLDRFKKKARKIPNNPLVSLTDIRSEVRLMKLTGVLKCSDMRSRELHKKQSTFPSSSSQRSNRRCQSCIAQKVIIKTGTKTFGDLCDIGIGQGFHFAREERLIKGQVLAPSGTSSSTVHTSPEYQSKTQAKGMKASRMKQEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3K262</accession>
    <name>E3K262_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_04387</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78431.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322850.2">
        <property type="nucleotide sequence ID" value="XM_003322802.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K262"/>
    <dbReference type="GeneID" id="10536029"/>
    <dbReference type="KEGG" id="pgr:PGTG_04387"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04387"/>
    <dbReference type="HOGENOM" id="CLU_1489708_0_0_1"/>
    <dbReference type="InParanoid" id="E3K262"/>
    <dbReference type="OrthoDB" id="10282248at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045182">
        <property type="term" value="F:translation regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000767">
        <property type="term" value="P:positive regulation of cytoplasmic translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="122"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="146"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP78431.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="181" mass="19758" checksum="7336DFEBBDCCB813" modified="2012-04-18" version="2">MPVDLAGHDQERILLADKVVQPCRPGGFPPGMGLGPARQSTPINLAADWHALINPCDQLICLLDDLWKTIRDHLAYSPNKISLDEAVGALESHEILKNIPLDHSDQLQSASTTVKGKGRPQCYTCGEKGHHLAECPKKKKFKSSLKSGEAQAGATSVPHLGNYDLNEDKDDSFDKEIDVWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2S2</accession>
    <name>E3K2S2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ATP-dependent DNA helicase sgs1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04597</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323060.1">
        <property type="nucleotide sequence ID" value="XM_003323012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2S2"/>
    <dbReference type="GeneID" id="10536494"/>
    <dbReference type="KEGG" id="pgr:PGTG_04597"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04597"/>
    <dbReference type="HOGENOM" id="CLU_1220209_0_0_1"/>
    <dbReference type="InParanoid" id="E3K2S2"/>
    <dbReference type="OrthoDB" id="2509459at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="160"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="236" mass="27558" checksum="4AD9CD0EC8EA0927" modified="2011-01-11" version="1">MAAGLINNMKEMTVDNFEDFITREWTTEEMKNLRKRKRVDNETITSVKHIKLMPDQRLVLSEVLRNAFDQLFARTYRNEILFGPDDLFRHEHITTLIDNLGTFKTVTELRKLIGGEVIAGQMEILLEAVDGYIKGPLAEDTQRRIDLARAEEERLISISKEEAEARARDEEVEREVARLEFQRIEEQRLLDLAKRLAREAAEKAWKEEQAEHMAMLVRQAGEDAERRGVKSIHWGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2W5</accession>
    <name>E3K2W5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04241</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78285.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322704.2">
        <property type="nucleotide sequence ID" value="XM_003322656.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2W5"/>
    <dbReference type="GeneID" id="10542071"/>
    <dbReference type="KEGG" id="pgr:PGTG_04241"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04241"/>
    <dbReference type="HOGENOM" id="CLU_669272_0_0_1"/>
    <dbReference type="InParanoid" id="E3K2W5"/>
    <dbReference type="OrthoDB" id="2498336at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78285.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="422" mass="46244" checksum="63B95CDAC9FF96B6" modified="2012-04-18" version="2">MPNISNMTPSPKQRNRFISTIRRTLRTNPQPASPTTPPFGKQKTPFATSIEPRASHASSSPSSSGSSDVSTPSPSNSSSQPTPQQRRYAAVFSKITFSTAEFIEDRYIYSQSPEKPEDPLSLATEELVPCPSFFAIPATRQPSVIDQPAVRLDQTSNPTEHRISFAGLSDLSTPTSPLRSCFQEVFDLIEAGLADNPFDLIGAEISDEPFNLLGVGRLTEPLDFPRATIRHRSIEADTPVIRLDIPAVGTSPESLELQESFDLPGIGASEKSFDLPGLGMPEESDSLGLDTSEDSFDLSGVATPDESFDHQEASMPIEQGGVTEHKAETVPSVDNSIQSFLYSLSASLRSDPTEFSPRISEWDQESNGFSWLNLDDEHDLNQPESLSELGNSRGRHTEYLSRPTFLKRSPARVKKLSSKQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>E3K383</accession>
    <name>E3K383_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="6">F-box and WD-40 domain-containing protein CDC4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_04896</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323359.1">
        <property type="nucleotide sequence ID" value="XM_003323311.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K383"/>
    <dbReference type="FunCoup" id="E3K383">
        <property type="interactions" value="104"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K383"/>
    <dbReference type="GeneID" id="10537128"/>
    <dbReference type="KEGG" id="pgr:PGTG_04896"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04896"/>
    <dbReference type="eggNOG" id="KOG0274">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_103_3_1"/>
    <dbReference type="InParanoid" id="E3K383"/>
    <dbReference type="OMA" id="KTTKIWF"/>
    <dbReference type="OrthoDB" id="190105at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001079">
        <property type="entry name" value="Cell division control protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849:SF1">
        <property type="entry name" value="F-BOX_WD REPEAT-CONTAINING PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00256">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="F-box" evidence="5">
        <location>
            <begin position="270"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="512"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="580"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="639"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="679"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="721"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="88"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="395"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="839"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="88"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="133"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="395"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="839"/>
            <end position="862"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP78940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="914" mass="101415" checksum="721C4E260A61AFB8" modified="2011-01-11" version="1">MSQPTTAFHFPPATQKTVITTTTTTTLNFPPLLIPQTQTQTNNNNNNDINLDQFPLAEATIPEDWTQRGLPLVFGNGSFAKFNPALSPSLPTASPSPSTHINPSNNSSSTTTTTTTTTTSSNNLNHPSRKRTTTSPQAPTNPTTNSSPPPSRSKKPKLEHTNQFYEPPSHLLPDHMQITPPDSTENLNNNHHHHHHQANNATTPLATVHPLQAANAHLELATSLSLPNLTAEFSRLPVALQQFTLFNLLKRSRLPVLQFVQQMIGPALKRDFICDSPPEIAVLILSKLDGTTLCRASAVSRTWYNMINNSRSIWKHRLKAENLWIGDGSEEIDAEECALVERGSKHWEISRFSRLWKAGVWNNPSSGSSPSSSSSSSAYQNQRLDKIGPARSSSIHPLYSDPSSSSSSLAVHNQSQPSISSSSDQESTIGDNQSDIKNNILSGSDSFKLLYRRRFLTRRNWMKKEPRRITFQGHALTVVTCIQFDWSKMVAASDDNVVHSYDLRTGRRLQNFIGHQGGVWALQYVGDTLVTGSTDRTVRVWNMRTGRNTHVFHGHTSTVRCLQIVEPVNVNPKTNEAPIWEPAHPVIVTGSRDYTLRVWKLPSETDEDYVGCPTPGSPDEIGPAASDTNSGNRFHLHFLRGHNHAVRALAAKGRTMVSGSYDCTVRAWDIMTGKCTQVMRGHQQKIYSVVLDGSRGRCASGSMDNTVRLWDLSTGETIRILGEHSSLVGLLGLSPRRLVSAAADATLRVWDPSTGACRYELKGHLGAITCFTHDEFRVVSGADGTLKLWNGENGVLVGDLLAGFSAVWQVSMDERFCVVAVQRGTESEYVVLDFGRSDGKVDREEPREEEPREEEPHEELREPSPPIPPIAPIPRHLRHLRHLHHNHRPISGEHAFLLYPDQHEVDHTPIEDLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3J2</accession>
    <name>E3K3J2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_05005</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP79049.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323468.2">
        <property type="nucleotide sequence ID" value="XM_003323420.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3J2"/>
    <dbReference type="GeneID" id="10530870"/>
    <dbReference type="KEGG" id="pgr:PGTG_05005"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05005"/>
    <dbReference type="HOGENOM" id="CLU_1636224_0_0_1"/>
    <dbReference type="InParanoid" id="E3K3J2"/>
    <dbReference type="OrthoDB" id="10270197at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP79049.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="162" mass="18031" checksum="5338E0685B1C64DC" modified="2012-04-18" version="2">MLDTSQTEVYCQDLAKRACSRSLEPSRTARLHGVLGQEIAKLACSRAELKTSQSEPFRDVLSQELAKPAFSRGLKSRPHEASPQDFAKPVCSRCLEFVGEASLFPMVFKTWRAGPSRRCCTHVASPRLAAVCVRHQTQSQETMISLTIIVQSMYHQSNVSDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3K4T5</accession>
    <name>E3K4T5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_05571</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="PAR_bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988:SF27">
        <property type="entry name" value="GH27708P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988">
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        <property type="match status" value="1"/>
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    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
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    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
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            <begin position="25"/>
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        </location>
    </feature>
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        <source>
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        </source>
    </evidence>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP79250.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="470" mass="53160" checksum="2BECAF8450F00187" modified="2012-04-18" version="2">MAKLSDLPAELVYRIIDYILPRDDPDPAHSEDRPIDRDDHHLLDHNGIGPNVPRPRPHLERLSRNPTPRSLYHDFRAVAASQPYASWPRGLPSNPLLPLSLVNRTFQRCAQEILFKNVPLLSRWRAIKFLETLTCQSIQHPSTSRRCNRKRAKNVQDENVRMLQLLKIDYSFQSSLAGHVRSLQLICNGMFSMAKGSGQLFCGIIHSCPLLEHIAISTAVSMDCKELILEALSSRRRIKEFVILDNFDNDSHSMFQWNVDDVVCRLFSQWDYLETVELSGLRGPSGPMIGTIEMPIPILNCAVRTMILHNPELDEQELSILLQTFAGSMRTLELNSPGHKINRAGLCRILNECTNAELECLTLDWSHYEQPISPVPDATNSDDPMTIDGLLDILFKSPSALQNLKSLSFTGTLATHALFERLPDSIVKLAWETAIFLRPRSLTYSQVQEMTTNSYRILSAVQSEVVTNGT</sequence>
</entry>
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            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    <organism evidence="2 3">
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        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79348.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K533"/>
    <dbReference type="STRING" id="418459.E3K533"/>
    <dbReference type="GeneID" id="10531172"/>
    <dbReference type="KEGG" id="pgr:PGTG_05669"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05669"/>
    <dbReference type="HOGENOM" id="CLU_031560_1_0_1"/>
    <dbReference type="InParanoid" id="E3K533"/>
    <dbReference type="OrthoDB" id="2506424at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79348.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="363" mass="40397" checksum="DCABD939507AAD14" modified="2012-04-18" version="2">MPPGTVNMPPDDHDDQPQYHEIEMTSSDVNTHNHDNSLISSTSTNPPLNQSRHNPDNQGVLKIISKLIMNPEADGSVIITPDKVQLLKSLIGVEEFINVKTLNMMDKITSRLDAIEKSMSKTVSPTKPPPSTWSSIVKKTSSPANNNIVRPPPSARIINEFKPSFFIIRKTVPEAQAFIQKSPAQITEKVNSVLSDMEAKTDDGTPITVKGAIILPSGDVKFFTPTRFATNWLLEHKHEWTHLCDPALVTPQTSFPVIIHTVPISFTPTNKATIADLCRENNGMDTKVIQSMRWLGNPQANKKAHGSIIMNLLDKEVSKKIERGGLFFKGLFFKGAHYKKSPMQCFQCLEVGHTAQLQHNPRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3K6A6</accession>
    <name>E3K6A6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05062</name>
    </gene>
    <organism evidence="2 3">
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        <lineage>
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            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EFP79837.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003324208.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K6A6"/>
    <dbReference type="GeneID" id="10533365"/>
    <dbReference type="KEGG" id="pgr:PGTG_05062"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05062"/>
    <dbReference type="HOGENOM" id="CLU_1138477_0_0_1"/>
    <dbReference type="InParanoid" id="E3K6A6"/>
    <dbReference type="OrthoDB" id="10364561at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171780" evidence="1">
        <location>
            <begin position="24"/>
            <end position="244"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79837.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="244" mass="27207" checksum="2729C2BE50F0C955" modified="2012-04-18" version="2">MSTFLGLRKTVLLALCCLQVAQSLPVENGHAASQLKDGNLDIGKAVIDPQDVSARLETNGLNKIDEKSTGISSSGQQSKNETGRLAKIENLDTQGNQEEIDAWKSAQETFHKYFENIYPSGLEPTYSYAGIPYGNFYTAESPHSGFVPSISVAKPLDQEQEKNISYQDILKLKIDAVTPKTSNPTTSRELAGRLSHMMHRIKLDLEDWRNFYSYLHEELAEELKEEGLGVDKLEAKTTEENPEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3K6E5</accession>
    <name>E3K6E5_PUCGT</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05101</name>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP79876.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
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</entry>
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    <accession>E3K7M0</accession>
    <name>E3K7M0_PUCGT</name>
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        <recommendedName>
            <fullName evidence="6">ZZ-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
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    <organism evidence="7 8">
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        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP80318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="418459.E3K7M0"/>
    <dbReference type="GeneID" id="10539326"/>
    <dbReference type="KEGG" id="pgr:PGTG_06274"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06274"/>
    <dbReference type="eggNOG" id="KOG4351">
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    <dbReference type="InParanoid" id="E3K7M0"/>
    <dbReference type="OrthoDB" id="2507445at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="2"/>
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        <property type="match status" value="2"/>
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        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ig-like_fold"/>
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        <property type="entry name" value="Nbr1_FW"/>
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    <dbReference type="InterPro" id="IPR000433">
        <property type="entry name" value="Znf_ZZ"/>
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    <dbReference type="InterPro" id="IPR043145">
        <property type="entry name" value="Znf_ZZ_sf"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20930:SF0">
        <property type="entry name" value="PROTEIN ILRUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16158">
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        <property type="match status" value="1"/>
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        <property type="match status" value="4"/>
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        <property type="entry name" value="ZnF_ZZ"/>
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        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01357">
        <property type="entry name" value="ZF_ZZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50135">
        <property type="entry name" value="ZF_ZZ_2"/>
        <property type="match status" value="2"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="ZZ-type" evidence="6">
        <location>
            <begin position="815"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="domain" description="ZZ-type" evidence="6">
        <location>
            <begin position="961"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
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            <end position="263"/>
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    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="633"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1093"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1436"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="223"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="633"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
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    <feature type="compositionally biased region" description="Low complexity" evidence="5">
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            <begin position="1144"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1505"/>
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    </feature>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1553" mass="171726" checksum="2FF12A429A8EAE12" modified="2011-01-11" version="1">MSNAQISSEKPQPHSFVFRTYKLDPKTKKPRPFNGRFLYMGGSASKSTFKALESRAQEIFPNAKHDLVLSIALGEPKPSLFPFNSFPKFEKIIEVIRVKPGGIPRDRLQRMLIDVWITTSTFSSPKPPAKLRFTRVEARAIKQQIDDALASLTNSNEPTDLAKTGATSRSLISDSFTDLEDIKNMIGDYSPLVPLDVTSYASVLKMKQAPLSSEPTGEAMESSMDSSVPTPDAVSSSGIQPCIESSQGHVEQPPTSLPEQPECNSNQLPEALVAQLDCFAGVDLHPTFHQTRNWYSILLEVFADDSRYVILPHTQSESLTLQVYFLNANSRAELVFMARNYPVGMLQSPYKRQQADDQMRSYLAEHISHVTIPKFHAVNVCDTTMSFYMVDCHSGRILPRRESNDSVDHILPDHHLMNLWKSELGTTAGDETLYSLISQVRSMVSQHEKIESWPSQLPELTAIEQSTYDHLSSLSTLNYVIREAAPSGRYRLAPLWHKSDSIHGATFATFCLVLYDVESGPVLIVQHSTDLGTSACRLKCDQLIRESFAHLAPACKTTNLHGVSLLNNQARFYNLNIATRLITPKTSPVDLFDRVLPDDYLSGAWDWRLQDNKFLEKFQMILADCQVTQSKVDPTAPATSTKPPLHRKTSPSPQLLGSQLSVISGHQVSGPQMRFICDGCDETISGFRAKCVHGTCPDFDLCAKCYERKDDVHPEHPFKLLIQRNNQDTLSSFAQKQWLPAVDPVCICDSCYCVIPGVVKKSPFSIGHDLCPKCLGDRKGPQDPKKVSEVAEETGYLEIEEDPISSAVPLASPPRVSIKCDSCSHTITGFRAKCSHDECPDYDLCSKCYELRRVFHPSDHSFKTFEVTVDAAGAVVSCNSHSDRPKTLGQQHTSDKTHFAKCDLCAQRISGVRWKCVDCVDWDSCEGCLKNVPAVHPFHRLVPIHDPNQLSHLPPQHLTNHHHVFCDGCDEPIRGIRYKCSHPDCPDYDLCSRCESSPIPRHDIDHAMLKIRDSYAWQAGKLRSKFTQTNHHRSPIALSSHPKKVKNATKVYQREAVTLNARVILNDHPVAHHQNSFDHPEFDSPVAVPKISVQQSPANPSSPSGCRSPSPQPWSNNHPVAHSQSSFDYPESKLYLPDFAGQESPVNPSSPSRCRSPSPQPWFNDHPVAHAQSSFEFDNRTDMPNFAGQESPVNPSPPSMCRSPSIRAETVLSFQLEPDVQDPGFESSTSPGPIVVVETPGELFVNSSPSCSGSPLEASQSDVNYHSNNLDAWEVPNTKATLGLPGAFPDESCSAVQAASQSSLDRVVVPSDDNEEETKPRFGAHFVSDLNLPDGTCVSAGARFTKIWLVRNTGSDKWPTGTQIAFNGGFHHSSQESFSVPAAVPDEVVEVSVETMAPEDSGGYMQVWRLVSPDGTRFGDRLWINLQAISEDQVNIDDPNSESLSTSVGFLLPNPSDRPLNDHQSESSQHSVTHPPGNNPTESSADSQQPQEELAPASSEFDSVDQPSSSSNLSSATDFDDLSYEFTSEHGDSEIGEDSFDFELVTDSDSSAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>E3K8F9</accession>
    <name>E3K8F9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Nucleolar protein 56</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_06555</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Required for 60S ribosomal subunit synthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOP5/NOP56 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325018.1">
        <property type="nucleotide sequence ID" value="XM_003324970.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K8F9"/>
    <dbReference type="FunCoup" id="E3K8F9">
        <property type="interactions" value="891"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K8F9"/>
    <dbReference type="GeneID" id="10539611"/>
    <dbReference type="KEGG" id="pgr:PGTG_06555"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06555"/>
    <dbReference type="eggNOG" id="KOG2573">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015495_4_1_1"/>
    <dbReference type="InParanoid" id="E3K8F9"/>
    <dbReference type="OMA" id="PDNYMFA"/>
    <dbReference type="OrthoDB" id="6780543at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000494">
        <property type="term" value="P:box C/D sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000452">
        <property type="term" value="P:snoRNA guided rRNA 2'-O-methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.90:FF:000001">
        <property type="entry name" value="Nucleolar protein 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.4070:FF:000002">
        <property type="entry name" value="Nucleolar protein 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045056">
        <property type="entry name" value="Nop56/Nop58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012974">
        <property type="entry name" value="NOP58/56_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894">
        <property type="entry name" value="NUCLEOLAR PROTEIN 5 NUCLEOLAR PROTEIN NOP5 NOP58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08156">
        <property type="entry name" value="NOP5NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00931">
        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Nop" evidence="8">
        <location>
            <begin position="292"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="434"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="495"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="546"/>
            <end position="555"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00040742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00056216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP80599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="555" mass="61133" checksum="3A3B46913D485A12" modified="2011-01-11" version="1">MATHVLFESSAGYALFAVKFTEEIAGRTKAVQEAIPNLSKFGKMIELKSFLPFRDAAHALENANDISEGLMSEHLQSLLELHIPAKKSSIVLGVAERGLAGSIKASLGIECDASDRSLELIRGIRLHAEKLLTGLEKGDMDKSQLGLGHAYSRAKVKFNVNRSDNMIIQAIALLDQLDKDVNTFAMRVREWYGWHFPELVKILPDSHQYASLVKFIGDKSKLTDESLEGITQLVEGNETLAKNILDAARSSMGTDISPVDLINILNFADRVIQLYDYRKSLQAYLREKMDLVAPNLGALIGDTVAARLISHAGSLTNLSKYPASTVQILGAEKALFRALKTKGNTPKYGLIYHSTFIGRAGTKNKGRISRFLANKCSIASRIDCFTDSPSTAFGQALRGQVEERLAFYETGANPTKNKVAMEAVLEKMKAAEEELKSSTTKESAKPKKRKSSALDGTDLADPAEETDKKKKKKKRKSDATEIAEGDVSMDVDTSVIKEKSKSDKEKSKSDKEKSKSDKEKSKSEKEKSKSDKEKSKSDKEKSKSEGKKKKKQSAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3K8P5</accession>
    <name>E3K8P5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BED-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06582</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80626.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325045.2">
        <property type="nucleotide sequence ID" value="XM_003324997.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K8P5"/>
    <dbReference type="GeneID" id="10539640"/>
    <dbReference type="KEGG" id="pgr:PGTG_06582"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06582"/>
    <dbReference type="HOGENOM" id="CLU_050093_0_1_1"/>
    <dbReference type="InParanoid" id="E3K8P5"/>
    <dbReference type="OrthoDB" id="2505040at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF5">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP80626.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="37478" checksum="C3B6634D7D7B7696" modified="2012-04-18" version="2">MPNQSSFLPSSTPAGCTSEVSEVQSDPEPKSKSQDKPVAKKKNGGAQQSLTQTESCQSQGIDLTQDSDQENSKAKRKCQRQNQEFDNVRDFFSAPFHRKDDPREIPPSTYSCKWCLKEVRVSATSFSNLRTHRDGSRQIGRISHGCPKRHEAINAGAKLPPTALDEERIKKAGGKAGTITHHFAPVKKFDNVVLNKIVTLWLLRQSIPWNRVEDEYLQAAFHYCQAGASLFKRKWAADSAKMVYLDLQDAMLKRLKDSDSKFNLIHDVWTTKGNRYGFIGASVSFIDNGWNYNVLHLSLKLVACHHKGSLLAKPVINILEKHQLQNKISLSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAC8</accession>
    <name>E3KAC8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fungal lipase-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_06906</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a diacylglycerol + H2O = a monoacylglycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32731"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17408"/>
            <dbReference type="ChEBI" id="CHEBI:18035"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a monoacylglycerol + H2O = glycerol + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15245"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17408"/>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AB hydrolase superfamily. Lipase family. Class 3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325704.1">
        <property type="nucleotide sequence ID" value="XM_003325656.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KAC8"/>
    <dbReference type="STRING" id="418459.E3KAC8"/>
    <dbReference type="GeneID" id="10536854"/>
    <dbReference type="KEGG" id="pgr:PGTG_06906"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06906"/>
    <dbReference type="eggNOG" id="ENOG502S1XT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029867_0_0_1"/>
    <dbReference type="InParanoid" id="E3KAC8"/>
    <dbReference type="OMA" id="YPLIFEF"/>
    <dbReference type="OrthoDB" id="426718at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002921">
        <property type="entry name" value="Fungal_lipase-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051218">
        <property type="entry name" value="Sec_MonoDiacylglyc_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45856">
        <property type="entry name" value="ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45856:SF24">
        <property type="entry name" value="FUNGAL LIPASE-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01764">
        <property type="entry name" value="Lipase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="138"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Fungal lipase-type" evidence="7">
        <location>
            <begin position="334"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="629"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="646"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00043996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00047591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP81285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="660" mass="74305" checksum="FED3AF66471BA79B" modified="2011-01-11" version="1">MSSSLTWLLHFEPKSAIMKIPTMDEALEHSKADRDELPDDCPFRYHDSKQWKQMSKGGDDEKAYYLNIWNSLSQLQKVVLSQHATDIIRASKKRQTFSSQIKATQTYCLLESAASLGWSSFVLLCGAPFALITRPFNVFYMVAYFGSVYSFLFVANVAFLIAHVLGIGKFIRMLGDRQGAGWSPINWLNINIFNRKQRIIKHAREAFCSELPSFEPATKIDDPHSKRRKLQFNVDVARSILLMCSIVYERDVRFVEKAASGAISRPSLGDSDSPSEPNEGVLYLLKSEEYMYRAGYLWDLRFVSMADFRKLTGPFAGAFYDYEKVGADENPFIVIVMKGTSPDNFSEWLMDCACKFESAGDFLATGLAHNGFYNTLFPSKTSDAHVLPYMRIVETIKMIAGQAAEYTQQKYGRAKKTNLYVGGHSLGAGIAQLLYARFLESPDDLGDQVVLRDGYVFGTPRACDFKLASRVDFNMNKPMNQGRQLWRVANRAQSPIIGDVVTRVPPGFADKREIRCGLQEGSYYAYAAIGTRVDLTPNALGPPFYRIEDAPVGHNVEVYKKADESENEFARARETSKFIMPRDIVQLSMWAVTYLVPILHDHFPASYMDSLYKVQAKTAYGKLKGNKIKSKSEQHTGHNFKTGQHPTAAAPTSTTPGRDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3KB93</accession>
    <name>E3KB93_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Oligopeptide transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_07855</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81606.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326025.2">
        <property type="nucleotide sequence ID" value="XM_003325977.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KB93"/>
    <dbReference type="GeneID" id="10533149"/>
    <dbReference type="KEGG" id="pgr:PGTG_07855"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07855"/>
    <dbReference type="eggNOG" id="KOG2262">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004965_3_0_1"/>
    <dbReference type="InParanoid" id="E3KB93"/>
    <dbReference type="OrthoDB" id="9986677at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004648">
        <property type="entry name" value="Oligpept_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22601">
        <property type="entry name" value="ISP4 LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="187"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="218"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="277"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="354"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="483"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="514"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="549"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="590"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="662"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="704"/>
            <end position="725"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="737"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="28"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="31"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="88"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP81606.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="796" mass="86831" checksum="2B6D404C07CFC281" modified="2012-04-18" version="2">MDSLLKTLGLRGSELKPLQLIKKISSVGCKSTSGSNATSTTAGTVTQRKGKKSQEIKEPLDFHSDYEKSQTAEKGQPIGTPASSSETLPHEEDIRSDKVSSEDELAMIPLTDDTESPIITVRSLLVGFLVAILGAAVTQLFLFKPVHMRLQPAFLQITAMLLGRAAALIPGPKWWNPGSFSMKETVFSSLIATSASVGAYAIELLAAQELFFDQVTSFGMSFGILLSSQLIGYGWAGLLQPILVYPTQVFYPEILPSVSLFYSLCGDGPVAKDQIKFFKRAFLAMGIYEIFPAYIAPAFQAISVFCLTLPKHQLITNIFGGAQPFEGLGFLNISGDWALVGAHGPLYTPLTAQVHHIVGVLLSLFILSFVYTNSWYDAGIKQSFPFMSVSLLSSNGTTYPIKTVINENGSPNEDVIQEIGIPFFTSTYVVVQIIASLAMTSAITHVLLENYPLILGIFKKNQEKVPVDPHRLVCQKYKDFPKWAFGLILISSIALAIGLSSLSGSGLPPLGMVFAIFLSSILTLAVGFLNAITGFHLHVSGVVQMFGGLLFPGNAFGNMWFTTYGASTVNQSMNMLKDLKLGQYMHLPQTFVVLAQMIGTIIGIFVNYGVMKTIIHSQREILLTSGGDGVFSGVAITSFQSSAVSWGAFAKRMFLADEKYGAIPLALVVGLFLPIPFFVLHRFWPRYNLNLINIPLLAGSIASGYSVATAGRFLNILIGLLSQFWARRYRPRWFMKYNYVLSAALDGGAQIMMLFLSMVFQGGDGRKVPFPIYFLNPDPSGPKDYCYMAPKVPLAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBT5</accession>
    <name>E3KBT5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_07979</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326149.1">
        <property type="nucleotide sequence ID" value="XM_003326101.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KBT5"/>
    <dbReference type="STRING" id="418459.E3KBT5"/>
    <dbReference type="GeneID" id="10533438"/>
    <dbReference type="KEGG" id="pgr:PGTG_07979"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07979"/>
    <dbReference type="HOGENOM" id="CLU_004591_1_2_1"/>
    <dbReference type="InParanoid" id="E3KBT5"/>
    <dbReference type="OMA" id="CGIATIP"/>
    <dbReference type="OrthoDB" id="2501240at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP81730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="852" mass="98050" checksum="C3190161D7A3D867" modified="2011-01-11" version="1">MDDEADMNPPGFIQHETENGILYRCDVCGPKLMQEKQKVRHKQSDAHKKRVNDRDNFPLHHKRAVTPDERRVEAPIEDPVINSLDFEPEQDTSPLLPNSPTTISMLLIQQLQQDKANEESSSEDQSSQINWKDWLEVELERIRGDLDLDNPRDEFDENEEDDIPGQEDQHEWTPFKSKMEFVGSILVGYTQNLLSRTLFDLICVILNGLCQLKIPTWSTIRRSQKRIRAHFQMEVIMKDSVWGMPCAALSSRCALQQDLANPLVAPFIDFYPEDPQGCNQYKLSQSKKWLEMPSSLQTQMCVNNKKHFYIFEPVKTYSNQILVPIFLYLINGELHAKCALPTLKRISHSKFQLEIEGDLTFDDPSLFLVHTKEFNKEYSEIKIDRDLLSTVCSNELYGMNNNISVKIDLPNPWRTKAGGKILRHVPISLYADDTSGNILKCWNKHLSFYFTLSGLPPNLSNQEFNCHFLATSNRAGVLELAEIIVDELNDLATNGFEAYDAAISEPVFVMTSVLFFLGDSPMHSEITNTPYPGSALNPCCICTLSAPSLAAKHRKDFMYKFLHLDRHGNVTRNRPRVWLETIKQTHKLFKVATEDTIVAFDTLSKEYGVKDRINEKFIEQQGIAKVKAKINDLKANKFLRLFNPFLRLIGFDGCLDTPVEILHVFLLGVVKYLVHDFVGKLSEKQKDELEGRIESFNTSSLNMPKLQPKYLVLHVKSLIGKEFKIFLQAAPFILFPFMDEDQREIWISLCTLSSYAFQTHINNMEDFQLNLRKHTANFFYRIIRVTAQWLNKPKFHILLHLADSIRRFGPATLFSTEKFESYNGVLRTASTHSNRICPGRDIAIKFANFHAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3KE14</accession>
    <name>E3KE14_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed DNA polymerase</fullName>
            <ecNumber evidence="3">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_08327</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase delta/II small subunit family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="3"/>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82371.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326790.2">
        <property type="nucleotide sequence ID" value="XM_003326742.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KE14"/>
    <dbReference type="FunCoup" id="E3KE14">
        <property type="interactions" value="360"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KE14"/>
    <dbReference type="GeneID" id="10529748"/>
    <dbReference type="KEGG" id="pgr:PGTG_08327"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08327"/>
    <dbReference type="eggNOG" id="KOG2732">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021763_1_1_1"/>
    <dbReference type="InParanoid" id="E3KE14"/>
    <dbReference type="OrthoDB" id="3763at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043625">
        <property type="term" value="C:delta DNA polymerase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.430:FF:000002">
        <property type="entry name" value="DNA polymerase delta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.430">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040663">
        <property type="entry name" value="DNA_pol_D_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024826">
        <property type="entry name" value="DNA_pol_delta/II_ssu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416:SF0">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18018">
        <property type="entry name" value="DNA_pol_D_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA polymerase delta subunit OB-fold" evidence="11">
        <location>
            <begin position="33"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="DNA polymerase alpha/delta/epsilon subunit B" evidence="10">
        <location>
            <begin position="186"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP82371.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="550" mass="61573" checksum="E12072103BA0CE3E" modified="2012-04-18" version="2">MAVSTRPPQTYDPLSRLRDEFVVPFKARSYAQQFAKVYFLRLSRLRERLKEKCLERWKAQTPCRVLDIDPSRISVVIGTLYCEMKLKPNVLEDLAREHHLGPALGPRKWVSTDEDSIMLEDESGRVRLVGIDREKFTLVTGIIVGVMGRETSKGDFQVHDLIYAGVPDQPRKPTGKKKEDLDQGLVALVSGLDLDNNNSSRTKARAETLVEWLNGQIGGDEERRLARSVGRLVIAGNLTRVPSVNEPPVQNSKEATKLKRSAYEPAISAPPPTDPADQLLSQLDLPVDLMPGPTDPTIQSLPQQALHRCLFPKATALRTAHPLNHGPNPWWAEIGGASFLGTSGQNLDDIYKYVVDEDRLSLATQMLEWSHIAPTAPDTLHCYPFRERDPFVLSELPQVYFVGNQPEFKTHLQEYGPPENRSRTRIILLPRFSRSGTIVLVDPISLVCQTVLLLPSLEKLPDEPDDLDSDGQDHLPPEKLVMEIDAVLPYKIISIPGVGVGLWKDSLSSSFHHLILCSCSSFPYLILRSSLNLDQLHKSTTSADHGSYWM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>E3KE68</accession>
    <name>E3KE68_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_09002</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82806.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327225.2">
        <property type="nucleotide sequence ID" value="XM_003327177.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KE68"/>
    <dbReference type="GeneID" id="10544513"/>
    <dbReference type="KEGG" id="pgr:PGTG_09002"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09002"/>
    <dbReference type="HOGENOM" id="CLU_3033442_0_0_1"/>
    <dbReference type="InParanoid" id="E3KE68"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP82806.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="55" mass="6353" checksum="136B436F4CEA406F" modified="2012-04-18" version="2">MEALECESIPGRRKLVQRLSYLVVPSTISKMPSLNPWFCGGKLWKQSLMLFKLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>E3KFG1</accession>
    <name>E3KFG1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_09956</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178284">
        <property type="protein sequence ID" value="EFP82988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327407.1">
        <property type="nucleotide sequence ID" value="XM_003327359.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KFG1"/>
    <dbReference type="GeneID" id="10544699"/>
    <dbReference type="KEGG" id="pgr:PGTG_09956"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09956"/>
    <dbReference type="eggNOG" id="ENOG502S9H8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041320_0_0_1"/>
    <dbReference type="InParanoid" id="E3KFG1"/>
    <dbReference type="OMA" id="SFIAYEW"/>
    <dbReference type="OrthoDB" id="2526823at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="121"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="477"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP82988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="503" mass="57035" checksum="476C1209DF185B9E" modified="2011-01-11" version="1">MTQSTQEEQEQQESETEQQQELNTNQESEEQQQQPSHSLRLPRNSTLLTARDSHNTNNKQKQKTSKARTTSSPEKPKQQQRKPKRVKRRENQEDEILARFQGSFHAVLTRLLRLITWPLKYILTILLPHTFAGLTATAILLSVLYLLLMTIQRFFSSRLILPSLSLLANPVALVGRSLSLVTPSLLSFYCSTLHAPFFCTDPKEDHQREQKIAQYARTVSDSAQKAADIFDSVLLLSNPNLLRLHQADILELAYALRWASALDNKDALAHQLAQLAELTRSLKDSLIDLNGQSLNAFSFIAYEFSRLGDLMEWVEKGEKKYTSETIGRNLSILFEHLTSEMDGLLGSIEGLIPVASRSTNLGLRVMEGLHRAQYELIGRREAQGVLRKLTDLSSFSGRQLRRDLALTSESIAALKRTWTSLEQLRADLLAFRNHVAHFRASFVGAHLADHQLEPLDELFSMRQIIQGFQNTLALLKSNSRHPNHPSSSEPNRLLPSESNHQHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>E3KFH5</accession>
    <name>E3KFH5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">eRF1/Pelota-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_09970</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic release factor 1 family. Pelota subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178284">
        <property type="protein sequence ID" value="EFP83002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327421.1">
        <property type="nucleotide sequence ID" value="XM_003327373.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KFH5"/>
    <dbReference type="FunCoup" id="E3KFH5">
        <property type="interactions" value="458"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KFH5"/>
    <dbReference type="GeneID" id="10544713"/>
    <dbReference type="KEGG" id="pgr:PGTG_09970"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09970"/>
    <dbReference type="HOGENOM" id="CLU_023334_3_1_1"/>
    <dbReference type="InParanoid" id="E3KFH5"/>
    <dbReference type="OMA" id="LKMIILC"/>
    <dbReference type="OrthoDB" id="2498233at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070966">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, no-go decay"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071025">
        <property type="term" value="P:RNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.870:FF:000004">
        <property type="entry name" value="Protein DOM34 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.870">
        <property type="entry name" value="Pelota, domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038069">
        <property type="entry name" value="Pelota/DOM34_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058547">
        <property type="entry name" value="Pelota_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004405">
        <property type="entry name" value="TF_pelota"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853">
        <property type="entry name" value="PELOTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853:SF0">
        <property type="entry name" value="PROTEIN PELOTA HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26356">
        <property type="entry name" value="Pelota_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159065">
        <property type="entry name" value="Dom34/Pelota N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="85"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="99"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP83002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="385" mass="42795" checksum="662E0EA6F0BEB656" modified="2011-01-11" version="1">MKEIRTQIEKNKSGFVTLKAENEEDMWHAYNLVTVGDEVTSIAVQKMVDETNSATGSEDSHQVKLNLTIEVKKVNYSGVEIPSSGDWNKKGPSLANQPTRTSLSGGTSLHLSGPISKRNEHVNLGTFHTLELEHQCEFTIIKGPDGWDSVHVQRLADATNTSKSAEVAAILADDTGRATVCLIGTHTSLIKQRIEVPLSKNKKPGQPADKTLDEFHKQVYNSILKHFNLSHLRMVIIAGPGNTKDIVFEAIFTQAVKANNQAIITSKSKFQRIYTPTIHLQSLNQILSTPAILNQLKDTKYSQEIQALNMFQKTMAEDVRRALYGEWHVDWAAEQTAISTLLISDSLFRNPDPEKRKKFIKLTESVQQFGGKVLVFSSMHSTGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3KG05</accession>
    <name>E3KG05_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_09229</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EFP83276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327695.1">
        <property type="nucleotide sequence ID" value="XM_003327647.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KG05"/>
    <dbReference type="STRING" id="418459.E3KG05"/>
    <dbReference type="GeneID" id="10544990"/>
    <dbReference type="KEGG" id="pgr:PGTG_09229"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09229"/>
    <dbReference type="eggNOG" id="KOG4585">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018552_9_1_1"/>
    <dbReference type="InParanoid" id="E3KG05"/>
    <dbReference type="OMA" id="MYKCASG"/>
    <dbReference type="OrthoDB" id="2506086at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="3">
        <location>
            <begin position="8"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP83276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="154" mass="18004" checksum="CD970EFD3FD1FA88" modified="2011-01-11" version="1">MQLHLNPSNYFDKGQYLLADSTYELSHTVIPAYKLTAANITINLQFNYCLAKARVRNKHTIGVLKSRWSSLREMRLHLYRRQHMSAYVAWLYSCRILQNILAGLGDQWAELDDHESLPPDDDSDEDNIDESAEDFRDRLTINCVCYNMYQRGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGF1</accession>
    <name>E3KGF1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08562</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83376.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327795.2">
        <property type="nucleotide sequence ID" value="XM_003327747.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KGF1"/>
    <dbReference type="STRING" id="418459.E3KGF1"/>
    <dbReference type="GeneID" id="10545092"/>
    <dbReference type="KEGG" id="pgr:PGTG_08562"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08562"/>
    <dbReference type="eggNOG" id="ENOG502SNAB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_921788_0_0_1"/>
    <dbReference type="InParanoid" id="E3KGF1"/>
    <dbReference type="OrthoDB" id="184880at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF110">
        <property type="entry name" value="RHODANESE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83376.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="303" mass="33544" checksum="FB3D477D112645E9" modified="2012-04-18" version="2">MMTQESPEIPGVGRKEDYALGRSSTESKRLSDQHEVLLKAAGGYIPVPLSIECEMLEAILDVGTGNGAWLLGLRQSGIIPNSVELVGVDVSDKMMPSIELGRKFGLNLQVKDLCEPLPSSWNGKFDYIHGRHVFIWIEPYKWLDVMCNLKRALKPGGTLVIMYPGEVPYDHKTEKPLAIDTAPRRLFSAFLQYTASAGLPRQAVASLPKLLLEAGFDSDSIVTTTKRVNLGSAEPDSALRPASAHHLDFLLELMYNRTSSPEDDFPSPGDHVGWEELLEGWQEATDAGFFYDLFILSARKPQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGS5</accession>
    <name>E3KGS5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_08686</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83500.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327919.2">
        <property type="nucleotide sequence ID" value="XM_003327871.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KGS5"/>
    <dbReference type="GeneID" id="10532178"/>
    <dbReference type="KEGG" id="pgr:PGTG_08686"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08686"/>
    <dbReference type="HOGENOM" id="CLU_2400769_0_0_1"/>
    <dbReference type="InParanoid" id="E3KGS5"/>
    <dbReference type="OrthoDB" id="2151417at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38849">
        <property type="entry name" value="SMALL SECRETED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38849:SF1">
        <property type="entry name" value="SMALL SECRETED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP83500.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="93" mass="10173" checksum="07964B57231C9748" modified="2012-04-18" version="2">MTRLAVDAEQGFNNAIGNQSTKFGIGKQLDNGKTCNKVLKLTGTVLGLQMEIKLGKADATRLRKLANEINKLQKNIDADARAPNQKQRSFLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHE4</accession>
    <name>E3KHE4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09432</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328138.1">
        <property type="nucleotide sequence ID" value="XM_003328090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHE4"/>
    <dbReference type="GeneID" id="10532406"/>
    <dbReference type="KEGG" id="pgr:PGTG_09432"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09432"/>
    <dbReference type="HOGENOM" id="CLU_090077_0_0_1"/>
    <dbReference type="InParanoid" id="E3KHE4"/>
    <dbReference type="OrthoDB" id="2497391at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="270" mass="30684" checksum="7D20615201381CFE" modified="2011-01-11" version="1">MQVPGSNPGGPTPGGAAQEKWRELCWSTDVADSGIKATTSSEEHPSRSSLNPTSLSLQKQEEPMTRPLPATNDLPSTKIKLHKHWNLRPGLALQIEDALKEKFLEIPHPIFDSLARLMMYKVDLYSCYKQANAFDPLRTQVLLREWFPQEVDWDQFFITMDLLKSTRHYFSKQDTPISFIGFTLEDAKALLQLGFSPPSASYLYSTSTWDPPKKQLALHLVTKMDQMRKAQGYTSLIFHAHSQDFPGPSENKFFSQTQPNDNTSMIWPGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHT2</accession>
    <name>E3KHT2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09570</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EFP83857.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328276.2">
        <property type="nucleotide sequence ID" value="XM_003328228.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHT2"/>
    <dbReference type="GeneID" id="10532545"/>
    <dbReference type="KEGG" id="pgr:PGTG_09570"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09570"/>
    <dbReference type="HOGENOM" id="CLU_2005012_0_0_1"/>
    <dbReference type="InParanoid" id="E3KHT2"/>
    <dbReference type="OrthoDB" id="2495554at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173596" evidence="1">
        <location>
            <begin position="23"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83857.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="125" mass="14119" checksum="99421266BEB5891A" modified="2012-04-18" version="2">MRFTNFFNLCMLLLIQSEAVYSRFVCEDMSKPKAKLGICVRHINIEKDSKVPNLPPLDKKDFLAIDATPKGTDRFTCDGLTLPTGGITARYCCDLKPDTKPRTLEKAELAMLCYSRDAKKKSHKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIW5</accession>
    <name>E3KIW5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10618</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84240.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328659.2">
        <property type="nucleotide sequence ID" value="XM_003328611.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIW5"/>
    <dbReference type="STRING" id="418459.E3KIW5"/>
    <dbReference type="GeneID" id="10545501"/>
    <dbReference type="KEGG" id="pgr:PGTG_10618"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10618"/>
    <dbReference type="eggNOG" id="KOG2914">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="E3KIW5"/>
    <dbReference type="OrthoDB" id="40579at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050308">
        <property type="term" value="F:sugar-phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000145">
        <property type="entry name" value="HAD family hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.240:FF:000023">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.240">
        <property type="entry name" value="Putative phosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051806">
        <property type="entry name" value="HAD-like_SPP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023198">
        <property type="entry name" value="PGP-like_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43481">
        <property type="entry name" value="FRUCTOSE-1-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43481:SF2">
        <property type="entry name" value="PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="377"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84240.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="396" mass="43296" checksum="06848348DFC44D2C" modified="2012-04-18" version="2">MDRWTAESLITMSQFNLCQSLNETNQAEMVVDAILFDMDGTLVDSIAAVESAWGAVAQTLNKDPQEVIDATHGRRAIDNLKDLKPHLRRMSNAEMEPHVEAFETEILRQADHFQNEVRSRRSSVASSKRQSRETSRKNSMVINGHDHKPRRKSSLAHFITADGVSGRMAALGLKPLTQAPVNGHANHEDPNSSNIDEDVFDDDDEDEEEIKTDDEEVDVSDLTDKSVRILPGVRELIDSLPEGRYAVATSGAKTYCYGALKRVGITPPKVTITADDPRLKNGKPFPDPFILAAECLGYEPGNCLVIEDSPSGIKAGVASGAMTIAVCTSHPPEKISECGAHWLVPTLELVKVEVRKSDGKLRIYVDAEKDAVLKARKHAVQSSAPPELSKSLAPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJD2</accession>
    <name>E3KJD2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_10127</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178290">
        <property type="protein sequence ID" value="EFP84407.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328826.2">
        <property type="nucleotide sequence ID" value="XM_003328778.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJD2"/>
    <dbReference type="STRING" id="418459.E3KJD2"/>
    <dbReference type="GeneID" id="10545835"/>
    <dbReference type="KEGG" id="pgr:PGTG_10127"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10127"/>
    <dbReference type="HOGENOM" id="CLU_1687553_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJD2"/>
    <dbReference type="OrthoDB" id="2506087at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP84407.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="156" mass="18233" checksum="0FB3094FED7896F0" modified="2012-04-18" version="2">MITDLGVKDNINKQAMENMREGKDQIMIDKIKRLDTEDDRDQLFSPIMRLKGFDGCLDTPVEILHVFLLGVVKYLTGDFMKGLKACHLERLMASWESFNKNSLNIDIINSVNMAKHHDSFLGKHFRIILQAAPFVFYQFMTEEQRQLWVSMFQDKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KK20</accession>
    <name>E3KK20_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10804</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84645.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329064.2">
        <property type="nucleotide sequence ID" value="XM_003329016.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KK20"/>
    <dbReference type="GeneID" id="10546314"/>
    <dbReference type="KEGG" id="pgr:PGTG_10804"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10804"/>
    <dbReference type="HOGENOM" id="CLU_2237922_0_0_1"/>
    <dbReference type="InParanoid" id="E3KK20"/>
    <dbReference type="OrthoDB" id="10557062at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173661" evidence="1">
        <location>
            <begin position="25"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84645.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="105" mass="12344" checksum="6A29D35FA5F3E663" modified="2012-04-18" version="2">MYASYFKMWLLLISPLRLIGRSNCAFPSTEETLELAFKDYDFLLKSRKLSTELSYGDEEYFSWLETGQKISGKHSQDEENIDKSLKEKKLKPKSASIKAQIWNFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3KMV9</accession>
    <name>E3KMV9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Type 1 phosphatases regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_11137</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Regulator of type 1 phosphatases which maintains protein phosphatase activity under strict control.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the YPI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EFP85808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330227.1">
        <property type="nucleotide sequence ID" value="XM_003330179.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KMV9"/>
    <dbReference type="STRING" id="418459.E3KMV9"/>
    <dbReference type="GeneID" id="10544385"/>
    <dbReference type="KEGG" id="pgr:PGTG_11137"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11137"/>
    <dbReference type="eggNOG" id="KOG4102">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_098333_0_2_1"/>
    <dbReference type="InParanoid" id="E3KMV9"/>
    <dbReference type="OMA" id="HKPRQFD"/>
    <dbReference type="OrthoDB" id="307488at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008157">
        <property type="term" value="F:protein phosphatase 1 binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004865">
        <property type="term" value="F:protein serine/threonine phosphatase inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011107">
        <property type="entry name" value="PPI_Ypi1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20835:SF0">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PPP1R11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20835">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE PPP1R11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07491">
        <property type="entry name" value="PPI_Ypi1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="98"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="62"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="98"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="126"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU367162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP85808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="171" mass="18353" checksum="1304896B4A22BAF2" modified="2011-01-11" version="1">MATENNPQSNLTAVRQNDRLAPARGSRTMVIDQDSSSHQGEDSQANNSSSAATLILRAGPLQDSQPRQPRVQWQENVIDNEGLGRKKSKVCCIYNKPKAFDESSDESDHSDSDCCPTRSSGARPCPSTTSTTQARLGSTSQPTPSHSLPTPPRSPNAYEAEPSKSKGKGKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KN86</accession>
    <name>E3KN86_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_11051</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EFP85722.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330141.2">
        <property type="nucleotide sequence ID" value="XM_003330093.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KN86"/>
    <dbReference type="GeneID" id="10544288"/>
    <dbReference type="KEGG" id="pgr:PGTG_11051"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11051"/>
    <dbReference type="HOGENOM" id="CLU_696642_0_0_1"/>
    <dbReference type="InParanoid" id="E3KN86"/>
    <dbReference type="OrthoDB" id="10332068at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="339"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="164"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP85722.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="396" mass="44127" checksum="238A1990CEC3D084" modified="2012-04-18" version="2">MIKPQSPLDSSTRPTPMTRTASTALNTQSQASHDQSSFMELATPAAQKSRQTSPFPPTYAAILASSSQKNSKALNKVTKSPEASTKPNIKCSGQLASFPPATSTYAAIVAASANRQLQNTIATVPDSEQLPLQSSPDLSWTLANKPSNLTSLDDDTDQIPGAVSDKSSTLSDLSSSEKDTENTQPMPSHKPKKRKRKRGCDMTPHQRQKRNQNLNKRRKGKRVKNSLNSFTTAVPPPGAQTIQRNVDHLDLFPAITQDFCQRISEYKRLLADYENDPNNLQKPPKVYACSPTKEENESALKTVKKSFYTIDSNYNKIYDKKTNELVAIVEFIPLEDLSKSQWNDYDFLCLFLHQCKEFISPVSSKSRKCGGIMWAMGWRKAYNGLEILGRYRNQAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNL0</accession>
    <name>E3KNL0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_11641</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178297">
        <property type="protein sequence ID" value="EFP85885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330304.1">
        <property type="nucleotide sequence ID" value="XM_003330256.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNL0"/>
    <dbReference type="STRING" id="418459.E3KNL0"/>
    <dbReference type="GeneID" id="10544467"/>
    <dbReference type="KEGG" id="pgr:PGTG_11641"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11641"/>
    <dbReference type="HOGENOM" id="CLU_040578_2_1_1"/>
    <dbReference type="InParanoid" id="E3KNL0"/>
    <dbReference type="OMA" id="ASKCEQF"/>
    <dbReference type="OrthoDB" id="2142040at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34706">
        <property type="entry name" value="SLR1338 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34706:SF1">
        <property type="entry name" value="VWFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP85885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="16775" checksum="CC8EFBF9A729E547" modified="2011-01-11" version="1">MSQQNRWNEAAVAVAGLADTLVKYDSDGIDVYFMNSDEHLCNATSAAEVTQLFRTVQPVGQKEARATNRAPIKPLNLIVITNGEGDDPDTLAHALAGFSDRLDLGRFPLTQLGVQFVQIGDERDATRFLQALDNELKSELGGRRDIITGPVFGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNV0</accession>
    <name>E3KNV0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_11731</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178297">
        <property type="protein sequence ID" value="EFP85975.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330394.2">
        <property type="nucleotide sequence ID" value="XM_003330346.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNV0"/>
    <dbReference type="GeneID" id="10539974"/>
    <dbReference type="KEGG" id="pgr:PGTG_11731"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11731"/>
    <dbReference type="eggNOG" id="ENOG502SQHU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128330_0_0_1"/>
    <dbReference type="InParanoid" id="E3KNV0"/>
    <dbReference type="OrthoDB" id="2505154at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP85975.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="149" mass="16163" checksum="DBDF2B8A6DCB8F9F" modified="2012-04-18" version="2">MGSPVIKAIYPVDGKIHETGSRTSVETTRQTSVGVQLGVDQYGTATLNRAQSVKHHGYTAPYVSASGVHTSALDLTMSEDSSLKDGVARSLYFAALLEFDSAADRSFEASLMVETQSQKELPFKLWSSPKTWVLDYDGKTELGQLNLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPE0</accession>
    <name>E3KPE0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">UTP23 sensor motif region domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_12121</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in rRNA-processing and ribosome biogenesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the UTP23/FCF1 family. UTP23 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178298">
        <property type="protein sequence ID" value="EFP86165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330584.1">
        <property type="nucleotide sequence ID" value="XM_003330536.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KPE0"/>
    <dbReference type="FunCoup" id="E3KPE0">
        <property type="interactions" value="676"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KPE0"/>
    <dbReference type="GeneID" id="10540174"/>
    <dbReference type="KEGG" id="pgr:PGTG_12121"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12121"/>
    <dbReference type="eggNOG" id="KOG3164">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053567_1_1_1"/>
    <dbReference type="InParanoid" id="E3KPE0"/>
    <dbReference type="OMA" id="CCMQALY"/>
    <dbReference type="OrthoDB" id="25675at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09865">
        <property type="entry name" value="PIN_ScUtp23p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006984">
        <property type="entry name" value="Fcf1/UTP23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057776">
        <property type="entry name" value="UTP23_sensor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12416">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN UTP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04900">
        <property type="entry name" value="Fcf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24779">
        <property type="entry name" value="UTP23_sensor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="UTP23 sensor motif region" evidence="8">
        <location>
            <begin position="234"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="183"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="248"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="275"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038503"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF24779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP86165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="290" mass="32463" checksum="D7F582CBD822A95B" modified="2011-01-11" version="1">MKTKRNKSNRKTMRVYNSIFKFREPYQVLFDADFAVTVTTQKMEVQSRLAAVLGGTVKPMITQCSIHHLVNLASDGSQVAQEAVDLARNLCERRKCKHGKIKASSIECIKGILGDTDNRLRYIVCTQDPHFRTYLREKIIGVPIIYINRSGTLILEEEGPATELRRKQLQEDKLHVPPEELEQLKSTDTGPDSGLPAQLIQGPSTTNPQQTQSSDTLSNGQTHSAHKLENKLLKKKKRRSGGPNPLSVKKKKSSASNRRRIPQEKSASKNTPNQKTSSKSSRKTPPGKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPJ0</accession>
    <name>E3KPJ0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_12181</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178299">
        <property type="protein sequence ID" value="EFP86225.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330644.2">
        <property type="nucleotide sequence ID" value="XM_003330596.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KPJ0"/>
    <dbReference type="STRING" id="418459.E3KPJ0"/>
    <dbReference type="GeneID" id="10540236"/>
    <dbReference type="KEGG" id="pgr:PGTG_12181"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12181"/>
    <dbReference type="HOGENOM" id="CLU_1856293_0_0_1"/>
    <dbReference type="InParanoid" id="E3KPJ0"/>
    <dbReference type="OrthoDB" id="2505150at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173780" evidence="2">
        <location>
            <begin position="24"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP86225.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="135" mass="14979" checksum="7FF379144888D6F4" modified="2012-04-18" version="2">MPSPQSIWLLFLASGLPLLQVLGAQETSPARQHMLEKRSFCAIPNKRKGCTVWKVDACCQACCHEYLYDGAPAYWGEACKCPPRYPPPPPTHTVVVVQQPAPAPPPPPPPPPPPPPPRLSLSRHHHHLLKVQLQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQ79</accession>
    <name>E3KQ79_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_12410</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178300">
        <property type="protein sequence ID" value="EFP86454.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330873.2">
        <property type="nucleotide sequence ID" value="XM_003330825.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQ79"/>
    <dbReference type="STRING" id="418459.E3KQ79"/>
    <dbReference type="GeneID" id="10540469"/>
    <dbReference type="KEGG" id="pgr:PGTG_12410"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12410"/>
    <dbReference type="eggNOG" id="ENOG502SBYH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004591_6_1_1"/>
    <dbReference type="InParanoid" id="E3KQ79"/>
    <dbReference type="OrthoDB" id="2506588at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP86454.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="301" mass="34719" checksum="D2A956E0DE8FE5E0" modified="2012-04-18" version="2">MSKNGFTAYDITIGQEVHCPNQLRTMEKTIENSKKLWTCAKKPFMNVEKLATESAKLAIRDQINLKFCKELFAFEAEKTAILQEGEELPHHMDQEIPQKLVDMEENQPKKMFNQFLDLKGFDMVMDTPVEILHGLLLGPVKYLFQDFMKGLDEDQQKELLALWNSFNTDSLNIPSIRPTSMVQYSSSLIGKDFRIILQAAPFIFFQFMTPSDINIWSSLCHLGSLIFQTHIEDMETYISDLSNHIDIFLNHVLQDTAQWVNKAKFHMLLHLPESILRFGPASLFATEKFESYNAIITLSVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3KR17</accession>
    <name>E3KR17_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Syntrophin, gamma</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13124</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178302">
        <property type="protein sequence ID" value="EFP86742.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331161.2">
        <property type="nucleotide sequence ID" value="XM_003331113.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KR17"/>
    <dbReference type="GeneID" id="10541166"/>
    <dbReference type="KEGG" id="pgr:PGTG_13124"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13124"/>
    <dbReference type="HOGENOM" id="CLU_031219_0_0_1"/>
    <dbReference type="InParanoid" id="E3KR17"/>
    <dbReference type="OrthoDB" id="2507786at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="449"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="208"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86742.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="609" mass="65492" checksum="4A01648B61C2891A" modified="2012-04-18" version="2">MEYGSNFLSEFDHLLGVPTSTVGSSSIDSSSRADHASPSLEFDFWGLANDDTPAKFSDALTPMTTLPGLQVDYPNANSQTGLSQNNLAAFNSLSENQDKEDAPGKVDPPKPESSCHTATSSRVRFDPPKGPRKQHRTGFRQLPGANGVIKRGRPSKNEYKSAGVEVDMYALKGITLNGPAKSLDEVKGGEAIAKRPRSTRKTSRKPKSSVSAAVKSKPTPGPSKASQKESRSQQVPTTSSQSFIDVANMPFDFSSLQGTSNPDSQLAESNAGFNEQGYMAALQAFDNEIAMQFNSGSHHLSQQNADASQWDPNSQLGLSSSQPSTDLQPLTNLHPMTNLQPMTNLQPMTNLQPMANLQPLTNSQPLQPFSNLESSANLQSFNYLQSSTDLQPFTSLQSSTDLQPFTSLQSSTDLQPSTILQSANDLQPSLDYQSMTDSQLMSEIQSLTDSLPLQPPPDFQPSSDFPALNPPEASYIPASVDLEPSSTFPPLSWDINSSQSYANPPAGLDGNLPYPVDLQYVTGTLDSANVYDQLSYPANPPVGLDGTLPYPADSQYVAGGILSYPDQSQYVAGGDLPYPESQYLAGTSSNSNVNDPSAYPNSVLQNFPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KT76</accession>
    <name>E3KT76_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_13872</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178307">
        <property type="protein sequence ID" value="EFP87501.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331920.2">
        <property type="nucleotide sequence ID" value="XM_003331872.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KT76"/>
    <dbReference type="GeneID" id="10538400"/>
    <dbReference type="KEGG" id="pgr:PGTG_13872"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13872"/>
    <dbReference type="HOGENOM" id="CLU_067168_0_0_1"/>
    <dbReference type="InParanoid" id="E3KT76"/>
    <dbReference type="OrthoDB" id="10297183at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174108" evidence="2">
        <location>
            <begin position="20"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP87501.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="242" mass="27004" checksum="B1EC107FE3E647CF" modified="2012-04-18" version="2">MFRFSILVLICSTLKYASATPTNYAVTIAPHPISFASAPILAPPIQFTLERNGHLRLRNRKVLESMPPETQHPTPPSMERGSHSTRIPSEEQLSQIKQFIRHHETGKPYTLKPDGTFAEIEKRDGTIKYYFGRVEVFDEEGCAICLVCSPVAYGLCAAGYVFSLPAGKFCYKSLSAVGSYSYNALSLIASAAYYELLLIGRSSYGTWEKIRDLRNPRVTPMNDCEDQLPGNHHQEGQRAVPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3KT95</accession>
    <name>E3KT95_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF676 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_13891</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the putative lipase ROG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178307">
        <property type="protein sequence ID" value="EFP87520.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331939.2">
        <property type="nucleotide sequence ID" value="XM_003331891.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KT95"/>
    <dbReference type="GeneID" id="10538419"/>
    <dbReference type="KEGG" id="pgr:PGTG_13891"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13891"/>
    <dbReference type="eggNOG" id="KOG4372">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027968_0_0_1"/>
    <dbReference type="InParanoid" id="E3KT95"/>
    <dbReference type="OrthoDB" id="273452at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007751">
        <property type="entry name" value="DUF676_lipase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044294">
        <property type="entry name" value="Lipase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482:SF62">
        <property type="entry name" value="LIPASE ROG1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12482">
        <property type="entry name" value="LIPASE ROG1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05057">
        <property type="entry name" value="DUF676"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="337"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="DUF676" evidence="4">
        <location>
            <begin position="10"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="405"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="448"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="293"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="417"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="457"/>
            <end position="472"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF05057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP87520.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="551" mass="61444" checksum="F8CFC50168D1024D" modified="2012-04-18" version="2">MAAARTTNPSPVHLVVIIHGLWGSPEHVGHLASTLEKISGRTTNSTPLSILIPGSIQWTNTYDGIDYCAEHVAQEIDLRRAELELDGKVLTKFSCIGYSLGGLIARFLVGLLHSRQPSFFEEVEPMNFNTFASPWIGMPKYKGILSSTIHFFGSRLLSRTGNQLYLTDKYHQLPASSSANPTDKPSKKKFPLLSFLAHPETNFYKALVNFKVVRIYANAINDRTVPFVTGAMEKFDLFQIAHQISKKSKIMSQSSSDLTESEVLELGGLELTLNPDCPSLIEKVQLVKLGPQSEKRKRAEPMEGTKKTNQEKVKRSLPKLPFFLKPSTYPYRAPLNYLAAGLSPVIAPIFLTYILCSFCYQSIQSRQRIKKYNVGELENHFSRLRRVGLLQELRQDLIEDLAGIGNEPSLEADPADEQTTLPSTNRAGPSIHPNSAETQQLIHLETPTSALHPPDQPNSVDQPSKSHQTQGTQYLTSLSSDAIKSITNDLGLSPLQIDMLDRLNQLPNLAKYFSFLDGIPNSHGAIIYRVDGLSDRRGKRIVSHWAERFVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KTM9</accession>
    <name>E3KTM9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_13412</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178308">
        <property type="protein sequence ID" value="EFP87626.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332045.2">
        <property type="nucleotide sequence ID" value="XM_003331997.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KTM9"/>
    <dbReference type="GeneID" id="10538688"/>
    <dbReference type="KEGG" id="pgr:PGTG_13412"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13412"/>
    <dbReference type="HOGENOM" id="CLU_1409431_0_0_1"/>
    <dbReference type="InParanoid" id="E3KTM9"/>
    <dbReference type="OrthoDB" id="2513087at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP87626.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="193" mass="21860" checksum="B4A7BE44BF3C1C8E" modified="2012-04-18" version="2">MSGQQAEEIEVEPYTTVIVSSDDLADLFIEAHNKLTELLEEEAGGFDRAPTRRHINSVPLVFEKTTKEIQTILVESVARKVNLLHEMLSDSISLQKLHTAHLRDGIKILQDIEETVEQISSLVHSSWTLRDRKPGCGFQIEEGPEELLKRYRAERVQSRLADKFYSLGDLLESHFALLPLPTHGVYRGSDRGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3KTS3</accession>
    <name>E3KTS3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Bifunctional purine biosynthesis protein ADE17</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_13546</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Bifunctional enzyme that catalyzes the last two steps of purine biosynthesis. Acts as a transformylase that incorporates a formyl group to the AMP analog AICAR (5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide) to produce the intermediate formyl-AICAR (FAICAR). Also catalyzes the cyclization of FAICAR to IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(6R)-10-formyltetrahydrofolate + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + (6S)-5,6,7,8-tetrahydrofolate</text>
            <dbReference type="Rhea" id="RHEA:22192"/>
            <dbReference type="ChEBI" id="CHEBI:57453"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:195366"/>
            <dbReference type="EC" id="2.1.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:18445"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="EC" id="3.5.4.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PurH family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178308">
        <property type="protein sequence ID" value="EFP87760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332179.1">
        <property type="nucleotide sequence ID" value="XM_003332131.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KTS3"/>
    <dbReference type="FunCoup" id="E3KTS3">
        <property type="interactions" value="692"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KTS3"/>
    <dbReference type="GeneID" id="10539158"/>
    <dbReference type="KEGG" id="pgr:PGTG_13546"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13546"/>
    <dbReference type="HOGENOM" id="CLU_016316_3_2_1"/>
    <dbReference type="InParanoid" id="E3KTS3"/>
    <dbReference type="OMA" id="IKHNNPC"/>
    <dbReference type="OrthoDB" id="6017153at2759"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00133"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003937">
        <property type="term" value="F:IMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004643">
        <property type="term" value="F:phosphoribosylaminoimidazolecarboxamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01421">
        <property type="entry name" value="IMPCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.140.20:FF:000003">
        <property type="entry name" value="Bifunctional purine biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1380:FF:000003">
        <property type="entry name" value="Bifunctional purine biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.440:FF:000001">
        <property type="entry name" value="Bifunctional purine biosynthesis protein PURH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.440">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1380">
        <property type="entry name" value="Methylglyoxal synthase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00139">
        <property type="entry name" value="PurH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024051">
        <property type="entry name" value="AICAR_Tfase_dup_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024050">
        <property type="entry name" value="AICAR_Tfase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011607">
        <property type="entry name" value="MGS-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036914">
        <property type="entry name" value="MGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002695">
        <property type="entry name" value="PurH-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005492">
        <property type="entry name" value="PRK07106.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00355">
        <property type="entry name" value="purH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692:SF0">
        <property type="entry name" value="BIFUNCTIONAL PURINE BIOSYNTHESIS PROTEIN ATIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11692">
        <property type="entry name" value="BIFUNCTIONAL PURINE BIOSYNTHESIS PROTEIN PURH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01808">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02142">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00798">
        <property type="entry name" value="AICARFT_IMPCHas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00851">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52335">
        <property type="entry name" value="Methylglyoxal synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51855">
        <property type="entry name" value="MGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MGS-like" evidence="13">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00050488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00050687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00054363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EFP87760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="628" mass="68058" checksum="A1949424602033BC" modified="2011-01-11" version="1">MSCPSNGVNPIALLSVSNKTGLIDIAQGLHQAGLRLIGSGGTAKAVRDAGLPISDVSEITGAPEMLGGRVKTLHPVIHGGILSRQTPNDLADLAAQSIHPISVVVCNLYPFSECISKTPPPTIEEAVEEVDIGGVTLLRAAAKNHERVTILCDPNDYSSFLNQCQSNGINGVKESFRKSLAVKAFSMTAAYDATISSYFRIKYNSADSIATAPLEIKNELRQRAQQLSLRYGANPHQAPAQAYVTEGKLPFSVLSGSPGYINLLDALNSFALVRELSEAFQPPIPAAASFKHVSPAGVAVGTSEPLTDVEIQVFGVDGIQDLSPLAKAYARARGADRMSSFGDFLALSHTCDVSTAKIISREVSDGVVAPGYEPEALEILRKKKAGKYCVLQMDPSFQPELIERRQVHGITLEQRRNDVKITPELFKDIVTEAKDLPASAITDLIVATLSLKYTQSNSVAYAFRGGIIGLGAGQQSRIHCTRLAGTKADLWWLRHHPKVLGMKFKPTTKRADKANAIDLYLTDAVWDNDDDEEEGVISTERKEWEAIFEEIPERLSKAERKEWMKKLDGVALGSDAFFPFTDNVRRAAKSGVKYIAAPGGSVMDPAVFKAADEAKMVYCKTGLRLFHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KUM9</accession>
    <name>E3KUM9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13783</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178310">
        <property type="protein sequence ID" value="EFP87979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332398.1">
        <property type="nucleotide sequence ID" value="XM_003332350.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KUM9"/>
    <dbReference type="GeneID" id="10535038"/>
    <dbReference type="KEGG" id="pgr:PGTG_13783"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13783"/>
    <dbReference type="HOGENOM" id="CLU_1750613_0_0_1"/>
    <dbReference type="InParanoid" id="E3KUM9"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="149" mass="16698" checksum="FE6CBBBF0D90FD4B" modified="2011-01-11" version="1">MRPMSQRLWKNDQENEEDPSDGIGDLCENIFTKEDHLEEDTNPQTVLKFSPHPQSDRAKKPLSIRPIKLVKQKPEPITASKADEPGETSLSVALSWSTHYTQQCGLEIVCSDLGTCLYLGTKYHTWHLLAGAGWGSPLSLEPPTKSYSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3KWT4</accession>
    <name>E3KWT4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF7729 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_14717</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178316">
        <property type="protein sequence ID" value="EFP88751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003333122.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KWT4"/>
    <dbReference type="STRING" id="418459.E3KWT4"/>
    <dbReference type="GeneID" id="10541702"/>
    <dbReference type="KEGG" id="pgr:PGTG_14717"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14717"/>
    <dbReference type="eggNOG" id="ENOG502S2I3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049056_0_0_1"/>
    <dbReference type="InParanoid" id="E3KWT4"/>
    <dbReference type="OMA" id="TTCTRNI"/>
    <dbReference type="OrthoDB" id="5588482at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056146">
        <property type="entry name" value="DUF7729"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24855">
        <property type="entry name" value="DUF7729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174197" description="DUF7729 domain-containing protein" evidence="2">
        <location>
            <begin position="22"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7729" evidence="3">
        <location>
            <begin position="83"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="241"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="257"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP88751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="398" mass="41304" checksum="F858CE52A26E58F5" modified="2011-01-11" version="1">MPTWITSLIISSITFTGSVIAQAGTNATTQLPSNLANLPGFDSTNNLTTASNHSLAGYNTTDSNTTTTHSNPGANLRIPTTISQSCQDFLGHLNSNPNITSCTAPLLNATASFASGPKSVTRDQVKKAFDGLCGSNSGSGCAPHMMYTILNQFSSACNLELQTGVEAVRMAYDVLYILTPYRVALCSKDPTTGDYCPSVIAAAVVNGTASPASGKSANSSASDFGAMVSSVFGDKLVTQLGGSSGPHPSLHTRDTVPQNSSGSNPSPGTSQQQVFNPNPDTYTNTNLAFLFLSAYLPDAALCKPCTQSVLAAYIGFEQATPHFGGLSSSGYLSGQLKLWQAVSARCGAPFIEAINQEAGIMSILTTSAAAAFNLLLKNSSSFALAALLFISIKSLNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KXU6</accession>
    <name>E3KXU6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14873</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178319">
        <property type="protein sequence ID" value="EFP89032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003333403.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KXU6"/>
    <dbReference type="GeneID" id="10542475"/>
    <dbReference type="KEGG" id="pgr:PGTG_14873"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14873"/>
    <dbReference type="HOGENOM" id="CLU_1982664_0_0_1"/>
    <dbReference type="InParanoid" id="E3KXU6"/>
    <dbReference type="OrthoDB" id="10461442at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="126" mass="13994" checksum="06E0B20D3FF13DF4" modified="2011-01-11" version="1">MAKWLLAHKANTDALVAEDGFMVALRYDVQVRTNTFAHRVLLTDGSLSVAEILVFRPKIAHSCYATARKFGELENWSPRQLGPDHGATEARKEAAGDQGQHPSYCSRTILPTSSPECNERPKDAWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3KY48</accession>
    <name>E3KY48_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">25S rRNA adenine-N(1) methyltransferase</fullName>
            <ecNumber evidence="4">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_15090</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N(1) position of an adenine present in helix 65 in 25S rRNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
            <location evidence="4">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the BMT2 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="4"/>
    <dbReference type="EMBL" id="DS178320">
        <property type="protein sequence ID" value="EFP89249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333668.1">
        <property type="nucleotide sequence ID" value="XM_003333620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KY48"/>
    <dbReference type="FunCoup" id="E3KY48">
        <property type="interactions" value="63"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KY48"/>
    <dbReference type="GeneID" id="10546886"/>
    <dbReference type="KEGG" id="pgr:PGTG_15090"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15090"/>
    <dbReference type="HOGENOM" id="CLU_649130_0_0_1"/>
    <dbReference type="InParanoid" id="E3KY48"/>
    <dbReference type="OMA" id="FHRTSKW"/>
    <dbReference type="OrthoDB" id="5954793at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016433">
        <property type="term" value="F:rRNA (adenine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03044">
        <property type="entry name" value="BMT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021867">
        <property type="entry name" value="Bmt2/SAMTOR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21008:SF1">
        <property type="entry name" value="25S RRNA (ADENINE(2142)-N(1))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21008">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SENSOR UPSTREAM OF MTORC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11968">
        <property type="entry name" value="Bmt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="383"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="49"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="65"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="127"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP89249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="423" mass="47815" checksum="B1E9A7C749CDEAD3" modified="2011-01-11" version="1">MVQLNNRRARGRPTRNSLKTEILSKVKLNRNKQSTTNSTTRNPSEHKPNSQSNSEIQALHLTQESSAAVSSSSSSLIEDVPAEIKRKTDQSKVSSDATDKKAKPSKQKSNESNDRGKRKDSVAVVVEGDDDDEDGDQEEENKKKIERLIRKHSKKIKDEPSKLISEFHRIEKRLSQPKLDPLTRHRLILEQKKLGGLEAYQTASKIGGALENGGETGKWCAQTLIELGFRPSGPPPTINKVTLLDVGAIDGKSYAKYTDWISTTSIDINPIDESVVARCDFFKFPLPKTEEQKFDVVCLSLVLNFIGDLKKRGEMIKRARRFLKPSGKLFIVLPLACLNNSRYLDFKRFRMILNYLGFKVIKQHNSSKLTYWLLEKEDLQSNTIDHPTESDPTTNSNHSKSSLKFPKVQIRPGLSRNNFCIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KYF0</accession>
    <name>E3KYF0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15520</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178321">
        <property type="protein sequence ID" value="EFP89341.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333760.2">
        <property type="nucleotide sequence ID" value="XM_003333712.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KYF0"/>
    <dbReference type="GeneID" id="10547063"/>
    <dbReference type="KEGG" id="pgr:PGTG_15520"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15520"/>
    <dbReference type="HOGENOM" id="CLU_104740_0_0_1"/>
    <dbReference type="InParanoid" id="E3KYF0"/>
    <dbReference type="OrthoDB" id="10273286at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174025" description="Secreted protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89341.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="25467" checksum="767278F66421B675" modified="2012-04-18" version="2">MSAYKLALVVISLLANHISLSAAAACDDRSKLDINECQAARDSIAYVPGQNVIKSVERSIQASSGKCMIQVLVVPTPSPVISKNEIDTTINDILACGTHAGKNDPVKNIQVMVSGIPDNYTDTYGPNQKMNTAVCDTDANGGKNEKQDCLDAFKKITTNNLGQLLDTHGQPSNHTYTTVKTCTVQIFSTNGLPVNEKLDKLSSSLNVILDTCHKQSGYLALHGGTEGLNGRLSVKTGAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KYH3</accession>
    <name>E3KYH3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15543</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178321">
        <property type="protein sequence ID" value="EFP89364.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333783.2">
        <property type="nucleotide sequence ID" value="XM_003333735.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KYH3"/>
    <dbReference type="GeneID" id="10547169"/>
    <dbReference type="KEGG" id="pgr:PGTG_15543"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15543"/>
    <dbReference type="HOGENOM" id="CLU_1023549_0_0_1"/>
    <dbReference type="InParanoid" id="E3KYH3"/>
    <dbReference type="OrthoDB" id="10496688at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89364.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="272" mass="30879" checksum="C1B89A39DBD8F93A" modified="2012-04-18" version="2">MESMTQRLIDENVSTTVTQILQDPNYSLTLEEKDNVEKYIASIPHLGMGGNRVESNAFEHGSSGKTRQEKQAADEEENDFGKKLENLDSLHKSLGEDVPTSSKNTAVMETKFKEIEQRRKGYIEATLQRVLYVYFWSAAIHPTYFTYCDHIEVERIQTKDNQYRQGSSLPPTLVDLNRKDRSNQPGPTEQNADRTFLCPLGTGPLQAKLRLTRIQAEGFVAALRSSRSPEPLINAAWAHRAAPDPYKHKLRLTRVESKPNESLRRSQSSRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZ19</accession>
    <name>E3KZ19_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">L-arabinitol 4-dehydrogenase</fullName>
            <ecNumber evidence="8">1.1.1.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_15693</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <reaction evidence="10">
            <text>L-arabinitol + NAD(+) = L-xylulose + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16381"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17399"/>
            <dbReference type="ChEBI" id="CHEBI:18403"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Zn(2+)</name>
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        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
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    <comment type="similarity">
        <text evidence="2 11">Belongs to the zinc-containing alcohol dehydrogenase family.</text>
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    <dbReference type="EC" id="1.1.1.12" evidence="8"/>
    <dbReference type="EMBL" id="DS178323">
        <property type="protein sequence ID" value="EFP89544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    <dbReference type="FunCoup" id="E3KZ19">
        <property type="interactions" value="93"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KZ19"/>
    <dbReference type="GeneID" id="10547513"/>
    <dbReference type="KEGG" id="pgr:PGTG_15693"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15693"/>
    <dbReference type="eggNOG" id="KOG0024">
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    <dbReference type="HOGENOM" id="CLU_026673_11_5_1"/>
    <dbReference type="InParanoid" id="E3KZ19"/>
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    <dbReference type="OrthoDB" id="2148442at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
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    <dbReference type="GO" id="GO:0050019">
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:sorbitol catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Medium-chain alcohol dehydrogenases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013149">
        <property type="entry name" value="ADH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013154">
        <property type="entry name" value="ADH-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002328">
        <property type="entry name" value="ADH_Zn_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011032">
        <property type="entry name" value="GroES-like_sf"/>
    </dbReference>
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        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045306">
        <property type="entry name" value="SDH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161:SF12">
        <property type="entry name" value="L-ARABINITOL 4-DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43161">
        <property type="entry name" value="SORBITOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08240">
        <property type="entry name" value="ADH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00107">
        <property type="entry name" value="ADH_zinc_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50129">
        <property type="entry name" value="GroES-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00059">
        <property type="entry name" value="ADH_ZINC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alcohol dehydrogenase-like N-terminal" evidence="13">
        <location>
            <begin position="74"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Alcohol dehydrogenase-like C-terminal" evidence="12">
        <location>
            <begin position="228"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EFP89544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="400" mass="43350" checksum="56477D8D397D6C56" modified="2011-01-11" version="1">MSQSTTELKVDPVFASINPALYDPKIVLESSEFKVLDDPVELSKCSAANENIACCYNDKKQIHMVKKPMPKVHPGQVLLHIRATGICGSDVHFWKHSRVGEKMVVRDECGAGHESAGEVVELGEGVTDLQIGDRVAIEAGVPCSKPTCEKCRTGCYNACPQMIFFSTPPFHGLLTRYHAHPACWVHKIPAHITFEEGSLLEPLAVALAGIERANVRLGDPALVCGAGPIGLVTLLACRAAGACPLVITDLSEARLNFAKRLVPSVTTLQIKPGTSEREVAAEVQKIMQCKPTVALECTGFESSITVAIYSVGFGGKVFVIGVGKDKVTLPFSHMSENEIDLQFQFRYANQYPKAVRLISDGVIDVKPLVTHRFQLDKAVDAFTTSCDITSGSIKVQITDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZ79</accession>
    <name>E3KZ79_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15567</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178324">
        <property type="protein sequence ID" value="EFP89604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003333975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZ79"/>
    <dbReference type="GeneID" id="10547659"/>
    <dbReference type="KEGG" id="pgr:PGTG_15567"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15567"/>
    <dbReference type="HOGENOM" id="CLU_1741468_0_0_1"/>
    <dbReference type="InParanoid" id="E3KZ79"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="150" mass="16730" checksum="77CCF5E0304627ED" modified="2011-01-11" version="1">MAAGNDTNAEGECAEKGDGQSEESQVSELRCPEEALAGLEDDSRRTQAGAKRECVRERDGQDLLDRKLVLVWFGCFVGSKTAGCSCSRLPTWGRFGKVEREARRSGESRHDFLQREHPGATIHRYMSQQIRAYVIIDWPHCCAKKTSVAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>E3L036</accession>
    <name>E3L036_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_16211</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178327">
        <property type="protein sequence ID" value="EFP89923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334342.1">
        <property type="nucleotide sequence ID" value="XM_003334294.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L036"/>
    <dbReference type="GeneID" id="10545738"/>
    <dbReference type="KEGG" id="pgr:PGTG_16211"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16211"/>
    <dbReference type="HOGENOM" id="CLU_018552_2_0_1"/>
    <dbReference type="InParanoid" id="E3L036"/>
    <dbReference type="OMA" id="ILMHNAV"/>
    <dbReference type="OrthoDB" id="2502344at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF85">
        <property type="entry name" value="GH03217P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="8">
        <location>
            <begin position="220"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP89923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="431" mass="49089" checksum="ECAB44C6F4A14B6C" modified="2011-01-11" version="1">MHKYSERQQILRDLFMIRIFLHQQEGDDLIDSTLKIPTIPSLGTISARNNHGRALVLDALFDDEATVSDLLHLVLSNRYLNDRRPPMPRDEFDLCQLFNMRDEDFKQAVRTTKSGFTWLLSQIAFNPIFHSGSFRPQLPVPHQLALTLERLGSNGNGASVGRISRNLCVGRGTVIKASRRVIRAINDMGPTYLRWPDKDRRKEISEVMKAEGFEGCVGFVDGTTIPLYQRPSIDGEVFFDRKKRYSINCQVICDCDRFITGYMTGWPGSCGNSMVFKRMKVHKEPDDFFDPGQYLIADSAYELGVHCIPAYKAPAAYIRENTEFNYCLARSQVRNEHTIGILKGRWASLQQLRLAIQKPSDMMEIIRWVNCCVTLHNMLAHLGDAWDVLEANMEDTGPARGEVHEDNARGHRDSIQSKCLDTNYALGILPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3L0Y4</accession>
    <name>E3L0Y4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_16487</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178330">
        <property type="protein sequence ID" value="EFP90209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334628.1">
        <property type="nucleotide sequence ID" value="XM_003334580.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L0Y4"/>
    <dbReference type="GeneID" id="10546351"/>
    <dbReference type="KEGG" id="pgr:PGTG_16487"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16487"/>
    <dbReference type="HOGENOM" id="CLU_592021_0_0_1"/>
    <dbReference type="InParanoid" id="E3L0Y4"/>
    <dbReference type="OrthoDB" id="2509609at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="445"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="10"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="445"/>
            <end position="487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP90209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="496" mass="57740" checksum="3AEC0BDE7989E4E2" modified="2011-01-11" version="1">MDGRTDSSDLQELERELESGRQRIKLYELENQKLQASIQGLKSKVNNQSSFIRALLVPALSQAIPPLQAGRDTHRWRDRIGFGRLDLRLERELLPRLRRGLVKMTHALQSTRLEELHFSEDPVTEIAWLEGIVKDAHQLKSSVLQPWHHSHTHFEFIEATKTMGLPNFSQIDQNANATYILEPYYYRELPWGVDYIRDGIIKLFKIHIKALQAEDLNLKPDKSGETALGEVLRLIDQMIWDFEHPRKVLQLKWQRTVREIEIMELAVIQSLKPLLLQNDTGIEGISNYKHLKLDRWTPRELMSQSEVELLENYLPLLKLCRMMLNKLCSPASNEPQLILRIRTEGLREMYYETEAIEDDIRKFTYTVLRLRSKKAVVRVRDYLAGFRFLLTCTQTHLQSFLEQSDAGDPALIQNSLEWCEMWTDQFTTAIHNFSRGPSFLFDSDESLSPDSDSFFDYDDDSSDEEEEEDDDDDGEDEDEGDDNDNGDGDAWRLHLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3L1K5</accession>
    <name>E3L1K5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_16017</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178333">
        <property type="protein sequence ID" value="EFP90430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334849.1">
        <property type="nucleotide sequence ID" value="XM_003334801.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L1K5"/>
    <dbReference type="STRING" id="418459.E3L1K5"/>
    <dbReference type="GeneID" id="10530324"/>
    <dbReference type="KEGG" id="pgr:PGTG_16017"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16017"/>
    <dbReference type="HOGENOM" id="CLU_1235563_0_0_1"/>
    <dbReference type="InParanoid" id="E3L1K5"/>
    <dbReference type="OrthoDB" id="10254418at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007272">
        <property type="entry name" value="Sulf_transp_TsuA/YedE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30574">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YEDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30574:SF1">
        <property type="entry name" value="SULPHUR TRANSPORT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04143">
        <property type="entry name" value="Sulf_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP90430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="224" mass="24656" checksum="72BB674964005C27" modified="2011-01-11" version="1">MMDSATLLESLKLPLIGGALLSLSSMNLMLDFSKVFGCSQALKSLQESYKLIRFHYFQSSNAQSNNNHILFNHQNLFIAAGLALSGSFLRLFMNFIERNLTHNERSIFDSSLLSNHVHHSRSDDHQVLKNVLLFTLAGLLSGAGTTLASGCTSGHMLCGMARGSKRSIVATCVFFPVAILTHRVLKPLLAQIGVFSQSDSMYQEYSESVSTIGSDQLTSSSNWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>E3L1M0</accession>
    <name>E3L1M0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Store-operated calcium entry-associated regulatory factor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Transmembrane protein 66</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGTG_16032</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SARAF family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178333">
        <property type="protein sequence ID" value="EFP90445.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334864.2">
        <property type="nucleotide sequence ID" value="XM_003334816.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L1M0"/>
    <dbReference type="STRING" id="418459.E3L1M0"/>
    <dbReference type="GeneID" id="10530339"/>
    <dbReference type="KEGG" id="pgr:PGTG_16032"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16032"/>
    <dbReference type="eggNOG" id="ENOG502QT6Y">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046802_1_1_1"/>
    <dbReference type="InParanoid" id="E3L1M0"/>
    <dbReference type="OrthoDB" id="20303at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006816">
        <property type="term" value="P:calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001256">
        <property type="term" value="P:regulation of store-operated calcium entry"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009567">
        <property type="entry name" value="SARAF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15929">
        <property type="entry name" value="STORE-OPERATED CALCIUM ENTRY-ASSOCIATED REGULATORY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15929:SF0">
        <property type="entry name" value="STORE-OPERATED CALCIUM ENTRY-ASSOCIATED REGULATORY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06682">
        <property type="entry name" value="SARAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-0109">Calcium transport</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="161"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="209"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="14">
        <location>
            <begin position="168"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EFP90445.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="265" mass="28721" checksum="102F9BA6A01DF4FB" modified="2012-04-18" version="2">MAFNSKVLLSDISTLTFFDGEQTTFRRTSPVDQLVCRGPGCKVFRPEVIQCYNKGGLGTDVNWKCEADLPAKLKLGRVEVGCEGWSNSQDPYVLKGSCALTYTLNLDTYSKYNTSAESSPAVAWVYWMVVILIAFLFAYPYIRSFLVRFLPWLDPILADSSSGGDGGGPPPPYMPDQPPPKSAPSTSQPWWSPFLSGVVSGTVTHLLRGADTRASDRPSTGFATAPSYGRSHPTYRGQGSSSDMDDSSNLGPIQTSTGFGGTNNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>E3L255</accession>
    <name>E3L255_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Dolichyl-phosphate-mannose--protein mannosyltransferase</fullName>
            <ecNumber evidence="14">2.4.1.109</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PGTG_16656</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-seryl-[protein] = 3-O-(alpha-D-mannosyl)-L-seryl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17377"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:13546"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137321"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + L-threonyl-[protein] = 3-O-(alpha-D-mannosyl)-L-threonyl-[protein] + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53396"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:13547"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:137323"/>
            <dbReference type="EC" id="2.4.1.109"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the glycosyltransferase 39 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.109" evidence="14"/>
    <dbReference type="EMBL" id="DS178335">
        <property type="protein sequence ID" value="EFP90630.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335049.2">
        <property type="nucleotide sequence ID" value="XM_003335001.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L255"/>
    <dbReference type="FunCoup" id="E3L255">
        <property type="interactions" value="199"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L255"/>
    <dbReference type="GeneID" id="10530530"/>
    <dbReference type="KEGG" id="pgr:PGTG_16656"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16656"/>
    <dbReference type="eggNOG" id="KOG3359">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008438_0_0_1"/>
    <dbReference type="InParanoid" id="E3L255"/>
    <dbReference type="OrthoDB" id="292747at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097586">
        <property type="term" value="C:dolichyl-phosphate-mannose-protein mannosyltransferase Pmt4p homodimer complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004169">
        <property type="term" value="F:dolichyl-phosphate-mannose-protein mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
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        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1900101">
        <property type="term" value="P:regulation of endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd23285">
        <property type="entry name" value="beta-trefoil_MIR_PMT4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000044">
        <property type="entry name" value="Dolichyl-phosphate-mannose-protein mannosyltransferase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003342">
        <property type="entry name" value="ArnT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036300">
        <property type="entry name" value="MIR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016093">
        <property type="entry name" value="MIR_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027005">
        <property type="entry name" value="PMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032421">
        <property type="entry name" value="PMT_4TMC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050">
        <property type="entry name" value="DOLICHYL-PHOSPHATE-MANNOSE--PROTEIN MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10050:SF51">
        <property type="entry name" value="PROTEIN O-MANNOSYL-TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02815">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02366">
        <property type="entry name" value="PMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16192">
        <property type="entry name" value="PMT_4TMC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00472">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82109">
        <property type="entry name" value="MIR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50919">
        <property type="entry name" value="MIR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 14 17" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="111"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="223"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="247"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="286"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="335"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="661"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="699"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="729"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="778"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="388"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="MIR" evidence="16">
        <location>
            <begin position="457"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="64"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="10"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007222"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022676"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00022737"/>
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    </evidence>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023136"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00045102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU367007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EFP90630.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="826" mass="94552" checksum="968E54EC657B954B" modified="2012-04-18" version="2">MRSSQERLPTRRTPYQTRDSSSNITRSSRKQDHGDDKVGLSIGLRHSDRTNTYSRSALTENYDDRVYQDDDESDDKRSSDYHFGDNKPRTDDSAHDLNRSNDLCAYRRKDFLLLLIIGLLAALLRFKNIADPPGIAFDEVHFGKFLSFYLKREYFFDVHPPLAKLMLAAHAWLIGYKGDFAFENIADSYIGSSVPYVGLRSFSAILGSLTPMLVYAIMKESGYPAFASTISACLIVFDNGHIVHTRLILLDAPLILFASCSMFAYIKFYKLRYNGFSRPWWTWLTLTGLALSLTISCKFVGLFTYFTIGSAVALDLWDMLDIRRGHPLDHIGRHVMARVTCLIFVPFCVYLFWFWVHFSVLTHSGTGDEFMSAAFQQTLIDSPLTLSAEEIRYNDTLLIQHRATKCFLHSHLHRYPLKYEDGRVSSQGQQVTCYPHNDTNNHWLVEATRPIPDSGRGQIVRHNDVIRLKHRLTDSYLYTHDVASPSMPTHQEFTTWPDVAQTDEAYNGTLWEVAIDDAHAGQQWKTKASHFQLVHHVTRAAMWTRNLPALPGWGFGQQEVNGHKMYHDKTLFWVASDIVRDNTTNHASRPPPPKPKSVHSRSFFKKYFELQVAMFQHNSDLTDSHPYASPPINWPFLLTGVSFWTSTPDLRRQVYMIGNPAGWWFCVMSLSVIAGVFGADQFARRRGYSPIETPVRNRLYRSGGFFIIAWANHYFPFFLMNRQTFLHHYLPAHLFSALVAGVVTNFVVTESINHPVSILGPTTRRRPRMVVTESPKETILLVGISIVILYLGFSFIAPLTYGTPGLDPSQVNRRRLLSTWTLHFAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>E3L348</accession>
    <name>E3L348_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="11">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PGTG_17245</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. UvrD subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="11"/>
    <dbReference type="EMBL" id="DS178339">
        <property type="protein sequence ID" value="EFP90973.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335392.2">
        <property type="nucleotide sequence ID" value="XM_003335344.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L348"/>
    <dbReference type="FunCoup" id="E3L348">
        <property type="interactions" value="279"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L348"/>
    <dbReference type="GeneID" id="10528388"/>
    <dbReference type="KEGG" id="pgr:PGTG_17245"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17245"/>
    <dbReference type="eggNOG" id="KOG2108">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004585_4_1_1"/>
    <dbReference type="InParanoid" id="E3L348"/>
    <dbReference type="OrthoDB" id="1470711at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000725">
        <property type="term" value="P:recombinational repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17932">
        <property type="entry name" value="DEXQc_UvrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001201">
        <property type="entry name" value="ATP-dependent DNA helicase UvrD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.486.10">
        <property type="entry name" value="PCRA, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013986">
        <property type="entry name" value="DExx_box_DNA_helicase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014017">
        <property type="entry name" value="DNA_helicase_UvrD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000212">
        <property type="entry name" value="DNA_helicase_UvrD/REP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014016">
        <property type="entry name" value="UvrD-like_ATP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070:SF2">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE SRS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11070">
        <property type="entry name" value="UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00580">
        <property type="entry name" value="UvrD-helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13361">
        <property type="entry name" value="UvrD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51198">
        <property type="entry name" value="UVRD_HELICASE_ATP_BIND"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51217">
        <property type="entry name" value="UVRD_HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 13" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UvrD-like helicase ATP-binding" evidence="15">
        <location>
            <begin position="12"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="domain" description="UvrD-like helicase C-terminal" evidence="16">
        <location>
            <begin position="292"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="606"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="761"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="939"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="778"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="820"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="864"/>
            <end position="873"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="14">
        <location>
            <begin position="966"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="33"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EFP90973.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="990" mass="110109" checksum="B08E1646546064FE" modified="2012-04-18" version="2">MTDAAEPQWLNSLNQNQLKAVQRPADGSLQILAGPGSGKTRVLTYRIAYLLRKCGISPPSLVAVTFTNKSAKEMQHRLAALIGGSLASEVILGTFHGMCVTFLRKHGSKIGLRPNWVICDRDQQLQYGKSALNDPQFSEQVQDQSIKPKTVVEAISKAKSQGYSPEEMLRQAETSYQKLMAALYRSYTDLLHADNCLDFDDLLLLGEKLFRSHPTIIAHIQHVLIDEFQDTNTVQYEIMRLLAHRGAVTVVGDPDQGIYGFRYAQAINLEKMTKDFPGTQVALLEENYRSSSSILKASLAVVQQDNERIDKNLYTSHPRVSSPMVRPFSNPIEEAAFIAQEIHRLVAHTGGQLDYNDFCILMRYNALSRNLETALKTARIPVRMIGAQKFFERVEVRDILAYLQLADNPTFSPAFERVINTPRRKLGLAAINAVKAASATHKISQMEVLVRAVRGKRFAGIQPAQTETFRKFVSIICNLQAMAQGGCPVAEIIDNLVERINYADHLQKTYGPDAVQRDQNIQELKAFATHLAKESQATSNSLPLSQLSHVEELHRSPVKPPNRPIFTMSDTDLDRLESMFNTENISLPDDIVEEITPLRQFLVESSLSTDTDTQESKDDNNQPRVTITTCHSSKGLEWPVVFVIAAEDGIFPFYRCSEPEEVREERRLLFVAMTRAQGLLYLTHSVERMQGAETRSQEVSIFLKKLIKQSSDKASPLDCIFTPKLPEVDSRLRQELSAVIGRPTAPDDVIKSRIEEYESKERKTFTIPDPSGKRFSYPGSSQESKQNYNRWAQNRGPTQIGRFSSSGVKPAANVPLRGSFRTTSGPPTFTSAANYDPNAQGSGNTSKPLINGSTGKFSSKPLRTLSQPTQPVKVSTLHKPFVPPKSQISSQEPIVVKTEQPPSSSCPPSNPATGNRSLDRLKAISNEFIADLKDNIPEGLFDDLSDFEDPIKPEPSTSTKPDPAPGRKRRKAAGRPAKSKKKTAAPAKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3L521</accession>
    <name>E3L521_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_17502</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIKE family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178350">
        <property type="protein sequence ID" value="EFP91646.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336065.2">
        <property type="nucleotide sequence ID" value="XM_003336017.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L521"/>
    <dbReference type="STRING" id="418459.E3L521"/>
    <dbReference type="GeneID" id="10531585"/>
    <dbReference type="KEGG" id="pgr:PGTG_17502"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17502"/>
    <dbReference type="eggNOG" id="ENOG502S1AV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_654043_0_0_1"/>
    <dbReference type="InParanoid" id="E3L521"/>
    <dbReference type="OrthoDB" id="21214at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008555">
        <property type="entry name" value="SIKE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39472">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39472:SF1">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05769">
        <property type="entry name" value="SIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="198"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="311"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="352"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="15"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="320"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="374"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="391"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="418"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP91646.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="427" mass="48726" checksum="9D109C5236D0E2D2" modified="2012-04-18" version="2">MAVMEDELQRLWQLLAELSSQLTQNREQTEQLKKQAQELKTQAIHTGTGYPLKRFNVDLSKEQFESELERMNAQLVIENQTLQHENRHLTILLKDHETTLEQIMNRFRNHAHSTQQHELELTKYYESILLSLLEKQQQQQQQRYPNDGHPTELDPNTFQLIQNLSILIQKAIRELSGEEPYLSEADELESQQALIQAELSHQQSQTPTSTEPPPSPFAFQDPFKNPSPPPSHQSSTHQPKIIDQEQRRKSMSVIDYRPRLRDPSSGGYVGKRNGSLSDHALLSAIESERLRRENACLREMLGIATDDCGPIQPSAVHQHPPTHHHHHHHQEHSLTGNLHTVVSQVIKEEDEDPLHDLNHPPPPPPINLDLHQILPPPPPPPPQQEQEESEQQVPKDQIGSSDQQAGSSSSDDNVDALQNHDSHQLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3L5H0</accession>
    <name>E3L5H0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_17795</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP91795.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003336166.2"/>
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    <dbReference type="AlphaFoldDB" id="E3L5H0"/>
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    <dbReference type="KEGG" id="pgr:PGTG_17795"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17795"/>
    <dbReference type="HOGENOM" id="CLU_2623176_0_0_1"/>
    <dbReference type="InParanoid" id="E3L5H0"/>
    <dbReference type="OrthoDB" id="10350436at2759"/>
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        <source>
            <dbReference type="EMBL" id="EFP91795.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
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    <sequence length="78" mass="8398" checksum="56C0E70C11BE3607" modified="2012-04-18" version="2">MGMKIPNGGSSVNTIEKGFEAKAGTEINFGRDASHAPLVKRHHQDCGTGYAVVGVPVYQPVVCTSCGEDHHHHHHDHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3L673</accession>
    <name>E3L673_PUCGT</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="3" type="ORF">PGTG_18138</name>
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <dbReference type="InParanoid" id="E3L673"/>
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    </feature>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP92048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
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    </evidence>
    <sequence length="260" mass="24325" checksum="15C4032BE42C817D" modified="2011-01-11" version="1">MQSSLIISVLIVCSGVIAVPTSRGSNYAATEPYGESESYAPTNSYSPPPKSYSSSVQHSSYTQKPSYTNTAVPQYSSGPGQFPRVHAPGPVRFPSGSVGAYPQYGGFGNGGGMLPGFGLGGLGLGANAQVAGGVGGLVEGGTGFGTGIGANALANVGTGLKAGARVGAGVGGPLVGAGANVNAAVGAGVGGPLVRAGANVHAAVGAGVAGPFVGTNANVHAAAGVAGPTSIAAQANVGAAVSAAPVAVGAAARVGAGVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3L725</accession>
    <name>E3L725_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RNI-like protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="5" type="ORF">PGTG_18335</name>
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    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Puccinia</taxon>
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    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18335"/>
    <dbReference type="eggNOG" id="KOG1908">
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    <dbReference type="GO" id="GO:0005096">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="Ribonuclease Inhibitor"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027038">
        <property type="entry name" value="RanGap"/>
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        <property type="entry name" value="RAN GTPASE-ACTIVATING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="LRR_RI"/>
        <property type="match status" value="5"/>
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        <property type="entry name" value="RNI-like"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="2" id="KW-0433">Leucine-rich repeat</keyword>
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    <keyword evidence="3" id="KW-0677">Repeat</keyword>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP92348.2"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
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    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1363" mass="148496" checksum="3BAC50C0CE890EFD" modified="2012-04-18" version="2">MVNSQDDEQQQKQQQQQQQEPRTETPATTPSEPIETTITTTINTDNHSPIDQPPTPRPNTPPHRKLPARKPVRSILKPSPQPQPKFNFKRDILSPFSNKLGYAALEESPLGAVVGAGGVGQGVQAAAGWVGNAWKRLGAATTASPTTTTAAATTTTTTEKLTAADHETQQQQRSSLIHPTNSNHPIEPSNDLPADQTISSPPSSTHSHQVLSPNSRFNHLPISSSTEPSHNLNQTNALAPPLHPPPPAGTNLQLSVSSLKKVHFIMSDLKIVYPISNTQPPSFDQLNRLRINQARKLLLANRNAAHHHPPTSPTTTTQPNPINSSPTINGWTARSLELFYDECCRTREEGWGIMKVREIIKQSAPLPPKVLDLTGVPLNRVGAAEVLGDLLSVDFGLKSLVLSSCSLEDHCLKPILHGLLVSGSLPSISFANNPKLRAKGWKLIAVFIKKAKALRNIDISDNSLDRRSVEYLVQALAGNIRKDRPDANISPNSTNLKLPDVTPVANVSLTQTNLKSHSLPRLTSASSISPTSLSFNGHDLTTHLTQDEDDGASLFPSAPLLREDHSPALQALSAISNWSSSDSSNLVSLRLDHCNLKNNQLDTLAHAIRLSKLKHVSFRNNRIFNLGAVSLAVMIKDWPTQSESTFNDGIGGKVDSTPDHHSDYPGHPGNESLDPPTSSMPTPFVSLNSVTARQSDLLAQRQMQGKTPLPPPVDELPPDKHSPKQTAEFGKPELAQKDSNQNVSALVKDEMKKAHEQRFKIKSKIDQLPTVGQLLTLDVKSNDLRSADVFYLAQVLKKNRTLKVLNLAENRIDPIGLAHLADALRYNTCLETLDLSRNPCCGPNLEGILALRTTCTINNSLKRIFLSQTDLSSQGSIAIAEFLPEMKNLIHLDLTENHGIDIAGVMALAVSAKMNTSLRCLDINIPPNDPDFAHLSQEILQSCVRNTELAQEMANQRGAQTTIAQPMLKSTVVKALVNQQQQQQQEHPLEAIGIKQQPASSTPGRKSKNPAAMSRAETVKKILTSTEETCKVLRDLISEDEQRKLRMLQEERKVLMIVECSELVRELLDQVKAGQFQIREALGSLMMDEALRSHAQSVHAESVAVCEYAEAVHREPDAVNRGSISSSPSFSDSKVVSPVSNVSHDPKIRNSELSVGNQESDFEDEETTPRSDSISKTENDSSETTEFQSTSEPRGQSSQSADSSSISAQHSGLSSSPNGGKKGVRQRTEVTIDSGKDSLRLDSPSSNGAPPRSPVESHSRSLTIEEGEVFRKGSVLGTVAGDDDEEVPGEVLKESILVAQVERTRRNSANLEDQADAALLAEEQDTENREQTESDDDDGDPNETDEQGLPGKPINDILNQHLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3L793</accession>
    <name>E3L793_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18243</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178364">
        <property type="protein sequence ID" value="EFP92418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336837.1">
        <property type="nucleotide sequence ID" value="XM_003336789.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L793"/>
    <dbReference type="GeneID" id="10538243"/>
    <dbReference type="KEGG" id="pgr:PGTG_18243"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18243"/>
    <dbReference type="HOGENOM" id="CLU_030899_0_0_1"/>
    <dbReference type="InParanoid" id="E3L793"/>
    <dbReference type="OrthoDB" id="10304959at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="428"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="550"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="224"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="428"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="447"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="504"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="562"/>
            <end position="571"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="571" mass="64512" checksum="30F747AF130D8A3A" modified="2011-01-11" version="1">MCNTIAVSASEGTRSEPSKTVKSTARKPKKQYKSPEVVPSEWDRDSVNSTKSDKPRTTAKDTTNQEKDIVGKEVRTNKPKVEKRTSEATTTKVKSTEGVTAVKPKDPIPSVDPFKSVEKVSKLIDKDSISHLSFKKNDRVDSIGSEQGRIGNLQGIALLTKDLTEDRSKTASDQGSASKTLPDNVSSAAITLLNGGQTFNRPISQSTQKKLDNYFIPQGRTGRSVSSRSSDHPNSSAPSESYQPSEGSDLDIITGATAQLWSKPKISPVPLGDNILLRYCPALHPLLQAVKLNREYYRKAERKNDKAMKIVALRAAAGLQNDLSVSMNKEEFIKVFNWDPMSEFDEFQKGEKGQAFLNNGKLAVTPTPPPEVQMTPPEFDTNLNQRLDYQRESYHPHQPEVYQPQQQMFYHQNGYYYPYPPANPYWHQTTYNQNGSTYPQHHPPPQQWNESSQVHQTEATVPQPLPEKNEMPNHDKNEHFQKRKKNNKSKEASKKPNWVPPVNPNRAPRYSSAASAREQRQRSYQLSIHQEMIRLNRTTQAQYQALESMRSQNAGVANRRRLQGEDQTQRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>E3L7U2</accession>
    <name>E3L7U2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_18616</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178368">
        <property type="protein sequence ID" value="EFP92617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337036.1">
        <property type="nucleotide sequence ID" value="XM_003336988.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L7U2"/>
    <dbReference type="GeneID" id="10538945"/>
    <dbReference type="KEGG" id="pgr:PGTG_18616"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18616"/>
    <dbReference type="HOGENOM" id="CLU_2543680_0_0_1"/>
    <dbReference type="InParanoid" id="E3L7U2"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP92617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="83" mass="8830" checksum="DDFAC95A6882ABB2" modified="2011-01-11" version="1">MAAGAETDSGDDETGSGNCGAPFICLPACILQGEFLRCTVCISCSTQHPVSHPPSGPGRNPRYMGVVQFIGHAVHEWVRDRET</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3L840</accession>
    <name>E3L840_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_18734</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178371">
        <property type="protein sequence ID" value="EFP92715.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337134.2">
        <property type="nucleotide sequence ID" value="XM_003337086.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L840"/>
    <dbReference type="GeneID" id="10539211"/>
    <dbReference type="KEGG" id="pgr:PGTG_18734"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18734"/>
    <dbReference type="HOGENOM" id="CLU_715968_0_0_1"/>
    <dbReference type="InParanoid" id="E3L840"/>
    <dbReference type="OrthoDB" id="10310463at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP92715.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="386" mass="44641" checksum="F53C196FEAD4BA4D" modified="2012-04-18" version="2">MPTPKINFMEEQFNSALSLWPTPRVPPTELENSLKASDHQSHPLAHLTSKDFEVNHISPVPPSPPLESMLLNWEKDSTQVNQVQGKPFECGVGTSSNPFDTLIQDPNISTPVHDEIEKDHSRLAQRPDIDVDLTNRIVQSIQRTMIHQDGAIRSNEVPLSEPVNEVINRSPLRESKKRSTAECSSLNDQVDGEILENSFIVGYNTIFLLKHHYDIFYIRAHSEIYIKLGNAARQCIVPHPYLPIGIYPGKETPAVIRVLNDSTNKPMDATQMLCPRYYVLIKWIYFLHEEWLNNFKIPTFMYRVLQERLLNWLDFEICGSDDQNFPIIGTKRLEKLEWNKDHQFRDTQIKLIFYFSQTDKSSTVFDTAAYLLEAFKAENSREFHFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8M5</accession>
    <name>E3L8M5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18818</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178375">
        <property type="protein sequence ID" value="EFP92900.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337319.2">
        <property type="nucleotide sequence ID" value="XM_003337271.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L8M5"/>
    <dbReference type="GeneID" id="10542709"/>
    <dbReference type="KEGG" id="pgr:PGTG_18818"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18818"/>
    <dbReference type="HOGENOM" id="CLU_100692_0_0_1"/>
    <dbReference type="InParanoid" id="E3L8M5"/>
    <dbReference type="OrthoDB" id="10281008at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174292" evidence="1">
        <location>
            <begin position="28"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92900.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="233" mass="26020" checksum="8F222A7E98220FDE" modified="2012-04-18" version="2">MKKFSCSPLWLTALIIAPILHLPSTACSTIPKASPVLGQPILSKRAVIVPPRFVIIPIFGPVSFTEIFNWGCPTPTDNNRVGHGGYESRVKQVATQAIDQAVVEQYAKAHPEMAKEVSLDLREILRLEEQSGQLVSQIDHARVIKARADLARFFEAHPELQAQAEDTFQEQVDALFRSRDSADWEAANLDQVASRDWAHLTPQDDSKYKPNSALSKFHSHIQGHNLPRYTLGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8W3</accession>
    <name>E3L8W3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="17">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PGTG_19001</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178378">
        <property type="protein sequence ID" value="EFP92988.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337407.2">
        <property type="nucleotide sequence ID" value="XM_003337359.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L8W3"/>
    <dbReference type="FunCoup" id="E3L8W3">
        <property type="interactions" value="249"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L8W3"/>
    <dbReference type="GeneID" id="10542882"/>
    <dbReference type="KEGG" id="pgr:PGTG_19001"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19001"/>
    <dbReference type="eggNOG" id="KOG2518">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008978_1_0_1"/>
    <dbReference type="InParanoid" id="E3L8W3"/>
    <dbReference type="OrthoDB" id="26491at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000729">
        <property type="term" value="P:DNA double-strand break processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09908">
        <property type="entry name" value="H3TH_EXO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09857">
        <property type="entry name" value="PIN_EXO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000011">
        <property type="entry name" value="exonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000002">
        <property type="entry name" value="Exonuclease 1, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037315">
        <property type="entry name" value="EXO1_H3TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044752">
        <property type="entry name" value="PIN-like_EXO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF65">
        <property type="entry name" value="DNA DAMAGE-INDUCIBLE PROTEIN DIN7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0267">Excision nuclease</keyword>
    <keyword evidence="8" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="16">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="15">
        <location>
            <begin position="138"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="379"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="501"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="609"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="382"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="405"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="520"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EFP92988.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="685" mass="76047" checksum="BEE9D5231E12AFF7" modified="2012-04-18" version="2">MGIQGLLPFLRSIQRESHLKNWAGKTLAIDGYVWLHRGAYHCAQELCLGNPTTKHVDYFVQKIHLLHRFGVSAYVVFDGDHLPSKKVTEDDRENRRRTALANAQKLLAQGDQKRAREEFVKAVDVTPRLAHDVILALRSMGVKYVVAPYEADAQLRYLEMKGEVHGIITEDSDLLVYGARNVLFKMDPSGHCIHICRDELGQVHDKRMGLWDERQFRQMAMLSGCDYLSSIPGLGIKKAHDLIRRHQTAERAIKATRLDGKLPVPPKYEQSFREAELTFEHQFVYDPTTRTMIPLTPLPESPPSPQALAGCGEKWPDQIAIDVAEGRVDPMTKEAFSAQSSTLKHLPPFHQLKTTIPKQLAPSRPALSSSGKQSTLNQFAFKSSSSTSKTVSSTPARSSGREGPTSPQVSKSITPVNGKPSKFFECQPSKQPNSICPSPPEADSESQGYIATQTTIEDLPAEDAVPLTQESWDAISELSTDDSADPKVDNLGRDIEFEKLIGEDLPSSQMHDPSTAPLISRSSSSTGSNKENCQPVFTDEQLDSSFCPSSSLEITNQARQDLSDGFISSSQPEECELTITKPRVTIITPNNHHSIAEVINGRRISEPISRQTRQLPLSSDPIPSSDDEEDAQIRVMDTGLNRRKAAQSTFQSHQPLKKDLSRVCTDPFVQAVHSRQTERIDDSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3LAJ4</accession>
    <name>E3LAJ4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_19523</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178398">
        <property type="protein sequence ID" value="EFP93569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337988.1">
        <property type="nucleotide sequence ID" value="XM_003337940.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LAJ4"/>
    <dbReference type="GeneID" id="10534777"/>
    <dbReference type="KEGG" id="pgr:PGTG_19523"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19523"/>
    <dbReference type="HOGENOM" id="CLU_058865_1_0_1"/>
    <dbReference type="InParanoid" id="E3LAJ4"/>
    <dbReference type="OMA" id="WHESPGS"/>
    <dbReference type="OrthoDB" id="2502106at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP93569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="349" mass="39219" checksum="02879D5B51DD3754" modified="2011-01-11" version="1">MTTSPTVLQNGSMPTTVTNLRSQLGASGIPSGNSAFARSIHDFTRVVLGIEAAKSDLPPAPPQSTLSTFETPSKEASTNTVILARFRSYLSENNLSDLEVGGRTKCIPVVCRQFFVQDMAHINIHHVSFAWAQSPDSPYNTWFASMILKHLTFAKNTGLLYKYAISPTDDTAANGQKIMFRWIHGRQGELRKSARSLNWRQIKADREQRSKRKKQLSDHRVDTCVALGVPATLTRIFMDPACTSDTEEDDAGNLYRLHVPWRSQELTQFAHKLDEATVERLREQKGARYVQKAKLLELRRRNPINVPQHVRVPIGLPQNCYAPIYVQSRGQVAQEVLNTHNPPCEFPAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3LB13</accession>
    <name>E3LB13_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19722</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178406">
        <property type="protein sequence ID" value="EFP93738.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338157.2">
        <property type="nucleotide sequence ID" value="XM_003338109.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LB13"/>
    <dbReference type="STRING" id="418459.E3LB13"/>
    <dbReference type="GeneID" id="10535108"/>
    <dbReference type="KEGG" id="pgr:PGTG_19722"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19722"/>
    <dbReference type="HOGENOM" id="CLU_980515_0_0_1"/>
    <dbReference type="InParanoid" id="E3LB13"/>
    <dbReference type="OrthoDB" id="10425822at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93738.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="284" mass="31880" checksum="5A6BD543F7C61A95" modified="2012-04-18" version="2">MSSQVNHSPQSYNHTVRSRSTSDSVLQTTASKRPAEDLEFPYPPGKSRRLEFMKCRIRMDHEACNLLSKRSSMAYHYYTTGDYSAAANEYKTFLSNRSWWKSSIKEGGSLIIIQSIFKLARCYIALSQFDQARITIQELHDIKSGTAIGWGHALFNELASLYSEIKGRSAEVQDAIKASLLSSFECIGHTMSCNGKHEAAVQLLSKTLTSLPIDVNNLDTDLKDLLVELHLSLAEGYTCLGQPTAALSTLEKVWNPHGNLCITWGHRLFKKMANLNWSLKKRDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3LBZ9</accession>
    <name>E3LBZ9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19977</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178436">
        <property type="protein sequence ID" value="EFP94074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338493.1">
        <property type="nucleotide sequence ID" value="XM_003338445.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LBZ9"/>
    <dbReference type="GeneID" id="10535773"/>
    <dbReference type="KEGG" id="pgr:PGTG_19977"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19977"/>
    <dbReference type="HOGENOM" id="CLU_044243_0_0_1"/>
    <dbReference type="InParanoid" id="E3LBZ9"/>
    <dbReference type="OrthoDB" id="10320857at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="338"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="345"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP94074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="385" mass="43086" checksum="52403F013C59C692" modified="2011-01-11" version="1">MALSTPLTHLSERLKNSPPSPEDLLGLEISTMELEKTAKLIQYYLTLLASEKAQRAADYQPSDWELLTPEEQRARALRRVEISENERVANLLLWNSVKRDHKNKRRKLLAELRNKRLAKMSATSNSSAATTPAAGTTPIAETAANSPNTGRTIQTSTAPYPQPLDRGAANQLEERVNRLKLITIQKKMAPVTVERPTEATLAENGSTPAVETPATARATQVMASTAKKSAEKAADAMDIDPQASSFSADTQLRPKSPDVRVLSKEERIQLLVKEHVALWKRFLVDQPLGALEGNRALLTQAQDSQRALQKLIPRAEVEEYVKGWNPWTEKKNLFPVLQQERSGKNRSSSSRSNSYKAPKMNDPRRWKRLMKACHTLESGYMNMED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPI6</accession>
    <name>H6QPI6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ferric-chelate reductase (NADPH)</fullName>
            <ecNumber evidence="3">1.16.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGTG_20853</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="17" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>2 a Fe(II)-siderophore + NADP(+) + H(+) = 2 a Fe(III)-siderophore + NADPH</text>
            <dbReference type="Rhea" id="RHEA:28795"/>
            <dbReference type="Rhea" id="RHEA-COMP:11342"/>
            <dbReference type="Rhea" id="RHEA-COMP:11344"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.16.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ferric reductase (FRE) family.</text>
    </comment>
    <dbReference type="EC" id="1.16.1.9" evidence="3"/>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EHS63871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890566.1">
        <property type="nucleotide sequence ID" value="XM_003890517.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPI6"/>
    <dbReference type="FunCoup" id="H6QPI6">
        <property type="interactions" value="167"/>
    </dbReference>
    <dbReference type="GeneID" id="13543203"/>
    <dbReference type="KEGG" id="pgr:PGTG_20853"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20853"/>
    <dbReference type="eggNOG" id="KOG0039">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017408_0_0_1"/>
    <dbReference type="InParanoid" id="H6QPI6"/>
    <dbReference type="OrthoDB" id="17725at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000293">
        <property type="term" value="F:ferric-chelate reductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000116">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF9">
        <property type="entry name" value="FERRIC REDUCTASE TRANSMEMBRANE COMPONENT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 14" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="97"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="223"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="263"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="296"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="321"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="15">
        <location>
            <begin position="361"/>
            <end position="511"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EHS63871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="684" mass="76888" checksum="7365C878D19836AA" modified="2012-04-18" version="1">MNPSSRVATDSGWWPYFRLSSPAAMGTSGLLTAGKMPSSIVADHQTSVPTSERLVCYISFSFARHHCPVLPMVESLPPFTAQAQKIAQRQTTTEASFFLLGSLLGVFVLVGLLRAPFTIIYFITGRFKQGWTLRRNSQTSEVTSFRNWRVKGSRFTISGLKPRRTQQLFIEYLYWYNRVALQSVWRCQLLTLAKLLIALLYIMVVILSLFWKNWRADEFLSRFRRPAFIATVQLPLMFLLAMKNNICGFLGVGFERINFLHRLVGRTVVLCSIIHAVLILKVQVFPLRELFKQSTFMFGMISLATMIFLTLTSIGPIRKRFYQCFLAVHVLGYILLLFALWKHVEATHLPILICCGCKAVDQICQALNTRFRTAMFTSLPGKITIIEVPDLGTGWRAGQFVFLRVYSPKYILEKHPFTIANAPATDSPYRTKNSLVLIAKATGDYTRRIHEIGLTKAAMESQSSDVKSPHNLRVQSISSADTKSELGLIDSTYDSQLLVSIEGPYGHMYHDMKSHETVVLIGAGVACTFVMSLFEEVVGSALKASAATRNILIYWTLRDIDMVEFFLEPIRENMRMAQAMDIAVQIKIHVTSTIGIYKPRPFRELTIIQRRIDLKEVVTEAVNTTLAEINALGRCDPACGIGIASASGFSSFSKALRDAALSVDKKTFTEIGGLTVHTEIFDNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPN4</accession>
    <name>H6QPN4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="5">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_20833</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="5"/>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EHS64136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890545.1">
        <property type="nucleotide sequence ID" value="XM_003890496.1"/>
    </dbReference>
    <dbReference type="STRING" id="418459.H6QPN4"/>
    <dbReference type="GeneID" id="13542918"/>
    <dbReference type="KEGG" id="pgr:PGTG_20833"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20833"/>
    <dbReference type="eggNOG" id="ENOG502TAJ7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003526_0_0_1"/>
    <dbReference type="InParanoid" id="H6QPN4"/>
    <dbReference type="OrthoDB" id="10261556at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022698">
        <property type="entry name" value="OrsD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF145">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12013">
        <property type="entry name" value="OrsD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="8">
        <location>
            <begin position="14"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="853"/>
            <end position="972"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="997"/>
            <end position="1150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1181"/>
            <end position="1207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS64136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1295" mass="143608" checksum="147C08EDCB89A3C3" modified="2012-04-18" version="1">MSEPTLSSPSTVKISCSEPGCVQSFADDKSLKKHQREVHQHSTLCTPYATSSAPFHVHRNQDGTFHCPTKHCTWSSPIPGTFQKHVKNCNPDSFNDRPKVLPLPPSSLATLIPAGNQITLTKEFPFLGFNTYAKTLVCLDCHCGVPTKEVAGHLRSSPHKINNIIPDAIIQAFKSINVTVPLENKRTPPRASQPWKPVEGLYLKLGIICAVPHRGSICGHAVQKESSMSNHFSDIHKDGQLHLVLIQLPKVLLQSLTSSSTVPGGWASNSQRTYVQHFSNSTIRYFPVEYSAPHPAIIPAPSAAPSESQSNTSVLAWEQLSSALNKLDVLGHKQAVTISSDDAHPALVSNFLNSSGIHAHINACTDLLQCSTKELHTKVHYPNLLATWKPCVEKWLIGCMTELTSVHDAVTMEALRESVNDPPSRRPIRALQQRSTAVQYASVAAEFIVFTLETTGQPLATHFWSASNRRLSEFAEKASASLSSIQSDKTDAQGQFDRALNFLFWNSTSFSASILKRSKVQSALEQFIALSMIKPDGSFFSPSNLTHTIAALQYVIRVATVFACLEGTEEIYAHPPSDIDPKDHHLLEQSAISTYFQFIYDEKLISPFFTIRNWMRLASTIVMNEKPPDPTYWADSEMTKLVIGPTQITITAVQRALRIAICSINSVIQHTLHGASFPDFLYSKLDDQSANTSLGFNYLVASANEQMYHFKLLKDWVLNGSSPPNVLSPAWTSAMRLENGHYSLNLDQLCSKKHAWDWLESADLILEHIYFIYHVGCGQPARGTEEAAMLIRNIDSAPRNIYWRETRFMFQTYYHKGQNISNRSKPRQVFLTADLSMHLHNYLAYIRPVQILVIIQPLKALVDQTVNDFKKLKTVTVQHFEKGNFIDPLAQVIIVTTNQAADSHFCNQLRSCLPTRIIIDEAHSFLEDGYRGYMPGVTALTQLSTQLILTTASLPHSQEDDLLRITFGLRRLITKRQPTFRPELNIEVLRSPAKWEQLSSVIQPLINSFLLGPKDRAMIFIENKNAVELVGPQIEAVIHHSDLPDDVAAAAVEKWTSGGARSIVATSGFGTGINYLHVRLVCIYGIPNRATANKAYQQLGRAGRDGHQAHIFFIPTVSDPHGDIDDFKKNLMNPAVCPANTFAQHQDNLNWSCRNFPTFLSCVCCSRLSANPALLPSTLSLSQPSSIPDWSPDPSAPSNSYNSSSTVSAQATLGAHVGSGLAQSRKRRRLEDTDPLADKLATSNTLAAESSLSITSLARSAEQARLQSMPDNRVNSNQINRDNSLISHELWDPGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQ14</accession>
    <name>H6QQ14_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_20960</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EHS64497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890411.1">
        <property type="nucleotide sequence ID" value="XM_003890362.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQ14"/>
    <dbReference type="GeneID" id="13542651"/>
    <dbReference type="KEGG" id="pgr:PGTG_20960"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20960"/>
    <dbReference type="HOGENOM" id="CLU_2961903_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQ14"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="59" mass="6449" checksum="413EC7A476FDBBF5" modified="2012-04-18" version="1">MNKVPKAYCRESGHWQTSGFHGQLWTRVSVHCGGAMTEWAHQQAEAGLRVAGPEAEAVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSJ2</accession>
    <name>H6QSJ2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MULE transposase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21806</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178294">
        <property type="protein sequence ID" value="EHS63730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889552.1">
        <property type="nucleotide sequence ID" value="XM_003889503.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSJ2"/>
    <dbReference type="GeneID" id="13541839"/>
    <dbReference type="KEGG" id="pgr:PGTG_21806"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21806"/>
    <dbReference type="HOGENOM" id="CLU_2528546_0_0_1"/>
    <dbReference type="InParanoid" id="H6QSJ2"/>
    <dbReference type="OrthoDB" id="1880067at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018289">
        <property type="entry name" value="MULE_transposase_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10551">
        <property type="entry name" value="MULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MULE transposase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="84" mass="9512" checksum="5C4515C97A65E994" modified="2012-04-18" version="1">MPLLHVVRMNSCNRLFTVAMCFLLAKKEANYMWALEQLLLAMDNHSPSVIVTNHEQAVINVIKKVYPNAPGYSCKDCECPSLDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSL1</accession>
    <name>H6QSL1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21825</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178295">
        <property type="protein sequence ID" value="EHS63733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889492.1">
        <property type="nucleotide sequence ID" value="XM_003889443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSL1"/>
    <dbReference type="GeneID" id="13541874"/>
    <dbReference type="KEGG" id="pgr:PGTG_21825"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21825"/>
    <dbReference type="HOGENOM" id="CLU_2575007_0_0_1"/>
    <dbReference type="InParanoid" id="H6QSL1"/>
    <dbReference type="OrthoDB" id="10268250at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003605606" evidence="1">
        <location>
            <begin position="22"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="81" mass="8818" checksum="171AF2227216C39C" modified="2012-04-18" version="1">MRTFGLQILILFFGTIKLCAGEIINCDRDPKACEERGGSRSHNPYCPGLPPSMQARCCDNGEISSRPGCHHGGLPSGRRMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSV2</accession>
    <name>H6QSV2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21880</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178300">
        <property type="protein sequence ID" value="EHS63841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889402.1">
        <property type="nucleotide sequence ID" value="XM_003889353.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSV2"/>
    <dbReference type="GeneID" id="13540598"/>
    <dbReference type="KEGG" id="pgr:PGTG_21880"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21880"/>
    <dbReference type="HOGENOM" id="CLU_2050809_0_0_1"/>
    <dbReference type="InParanoid" id="H6QSV2"/>
    <dbReference type="OrthoDB" id="10368416at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="120" mass="13322" checksum="6BC28CFE9EAB6778" modified="2012-04-18" version="1">MADMSSSATMNRFQEVCHALLRMVEERSPGLSQTLPSSFPGVDDTSRMSRSQLGVLISELQAVGWGALKRMDRLLKTDKHSKSFVTNSTELSMGLFKERCYEVVGILEEGAILAVRVQLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSY5</accession>
    <name>H6QSY5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21956</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178302">
        <property type="protein sequence ID" value="EHS63939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889370.1">
        <property type="nucleotide sequence ID" value="XM_003889321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSY5"/>
    <dbReference type="GeneID" id="13540695"/>
    <dbReference type="KEGG" id="pgr:PGTG_21956"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21956"/>
    <dbReference type="HOGENOM" id="CLU_2850767_0_0_1"/>
    <dbReference type="InParanoid" id="H6QSY5"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="65" mass="7270" checksum="E94DB04EC1DAF86F" modified="2012-04-18" version="1">MAESSGNVIISGKDLLGKLISGAESQQSSSSPERIQAENTVREMNKPLQYHDEPIEIQYSLYWKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>H6QT46</accession>
    <name>H6QT46_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21926</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178305">
        <property type="protein sequence ID" value="EHS63995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889317.1">
        <property type="nucleotide sequence ID" value="XM_003889268.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QT46"/>
    <dbReference type="GeneID" id="13541951"/>
    <dbReference type="KEGG" id="pgr:PGTG_21926"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21926"/>
    <dbReference type="HOGENOM" id="CLU_039964_0_0_1"/>
    <dbReference type="InParanoid" id="H6QT46"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="274" mass="30821" checksum="174CF7601CAB5C5C" modified="2012-04-18" version="1">MSHFGIDLFGRENPPPGYHSASELWRIQNLGPPPDQLATQTIDLSTSFKLVYPDKNSNNKLTWIPRKSKGKFLISIPAISTTFEQFREIVARKCKENSEGAGVIVQNALESGSPGINCKVWMNLPAAHKFKKKANYKVNNINSFTHWTATIIANGKDRTNASLEVKMISPANLEKEAKAAVKIKRHVMTHASTSKHQDLSTLVAAEDFDIYNVYKNQIFDAHPPNVKYHKRIPVFIDPANETCYILLTTVNVEKWAKALVNETQYYSFNSLATT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>H6QT85</accession>
    <name>H6QT85_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22003</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178308">
        <property type="protein sequence ID" value="EHS64039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889288.1">
        <property type="nucleotide sequence ID" value="XM_003889239.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QT85"/>
    <dbReference type="GeneID" id="13542016"/>
    <dbReference type="KEGG" id="pgr:PGTG_22003"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22003"/>
    <dbReference type="HOGENOM" id="CLU_2334664_0_0_1"/>
    <dbReference type="InParanoid" id="H6QT85"/>
    <dbReference type="OrthoDB" id="10277327at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="98" mass="10834" checksum="A024476D92CE3CFF" modified="2012-04-18" version="1">MKLVRSQSCKVLRLYEHDPLKETTGSAKRLCIDSRELRDLGGPNETEFNAVEWACVVYLVGLEAPALPRGPSAGWASRVNACLRPQDFDFVRDADGIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>H6QU81</accession>
    <name>H6QU81_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22332</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178331">
        <property type="protein sequence ID" value="EHS64544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888949.1">
        <property type="nucleotide sequence ID" value="XM_003888900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QU81"/>
    <dbReference type="GeneID" id="13541153"/>
    <dbReference type="KEGG" id="pgr:PGTG_22332"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22332"/>
    <dbReference type="HOGENOM" id="CLU_003292_5_0_1"/>
    <dbReference type="InParanoid" id="H6QU81"/>
    <dbReference type="OrthoDB" id="5149081at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052925">
        <property type="entry name" value="Phage_Integrase-like_Recomb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34605:SF3">
        <property type="entry name" value="P CELL-TYPE AGGLUTINATION PROTEIN MAP4-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34605">
        <property type="entry name" value="PHAGE_INTEGRASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="312" mass="34696" checksum="97DB651C5AF9C51D" modified="2012-04-18" version="1">MGYKWNTLLSYNAAARKFMKYKIAVKETRFVFPILAGDLYGFCYWAGKNIDEYDEQDVCAKTLAKYLYGIQAWHLYHSVEYPAESKDRITVLLQASAHADAESPPRPVKAPVTMAQLVALTDHLVSGDDQSKAVLDLAIVAFWGMARLAEVTYDCATGPLQRTASLMTTDVRFVETQSSTVVELTIRGAKTCSPGESQKILLHSMAHMLCPVMAVKRRLGEARNEDISLFGHGNGLRGRSHLTKSSVRKVLSSSWNRLGFPNLTGHLFRVGGASLHCALGVPVAEICRLGRWTSGCYKLYLRVYQEEDVLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUM8</accession>
    <name>H6QUM8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22492</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178347">
        <property type="protein sequence ID" value="EHS64740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888777.1">
        <property type="nucleotide sequence ID" value="XM_003888728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUM8"/>
    <dbReference type="GeneID" id="13541616"/>
    <dbReference type="KEGG" id="pgr:PGTG_22492"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22492"/>
    <dbReference type="HOGENOM" id="CLU_1579302_0_0_1"/>
    <dbReference type="InParanoid" id="H6QUM8"/>
    <dbReference type="OrthoDB" id="2287304at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47150:SF5">
        <property type="entry name" value="OS07G0546750 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47150">
        <property type="entry name" value="OS12G0169200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="169" mass="18785" checksum="68BE9389B03506B9" modified="2012-04-18" version="1">MQRGLFLKIVKDIKQHDMYFAHKANTLGNLCLRGIQKITASLRILAYGGSHHATNKYICIGESTASKSLYKFYPIRYGIGQFIGSDRFFVTRYRKKGYWIAHPIRYPEYRIAAGSIAGLGIGIASRYRSDTDPSNDSGSGCGSSGETPTNRRAGRERRVAEAGVELLSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUQ8</accession>
    <name>H6QUQ8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22487</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178349">
        <property type="protein sequence ID" value="EHS64770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888761.1">
        <property type="nucleotide sequence ID" value="XM_003888712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUQ8"/>
    <dbReference type="GeneID" id="13541645"/>
    <dbReference type="KEGG" id="pgr:PGTG_22487"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22487"/>
    <dbReference type="HOGENOM" id="CLU_3093156_0_0_1"/>
    <dbReference type="InParanoid" id="H6QUQ8"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="52" mass="5975" checksum="5018B7609C9D2B31" modified="2012-04-18" version="1" fragment="single">QNMEEGPCGAVSMRLHKWIRNFVTVATWGPYLFNFTRQTGFLSMATFNAMTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUS2</accession>
    <name>H6QUS2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22507</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178352">
        <property type="protein sequence ID" value="EHS64830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888738.1">
        <property type="nucleotide sequence ID" value="XM_003888689.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUS2"/>
    <dbReference type="GeneID" id="13542058"/>
    <dbReference type="KEGG" id="pgr:PGTG_22507"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22507"/>
    <dbReference type="HOGENOM" id="CLU_2590915_0_0_1"/>
    <dbReference type="InParanoid" id="H6QUS2"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="80" mass="9150" checksum="916E5FD56E984065" modified="2012-04-18" version="1">MPLKVKRQTLAHRIKSCSHFPASGASRGCSPDWSLHRWTDRLRLLDAINLHVRMLIYRSRSIACAAFSESFDCYNGGRND</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUZ7</accession>
    <name>H6QUZ7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Trafficking protein particle complex subunit 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_22563</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178365">
        <property type="protein sequence ID" value="EHS62610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888673.1">
        <property type="nucleotide sequence ID" value="XM_003888624.1"/>
    </dbReference>
    <dbReference type="STRING" id="418459.H6QUZ7"/>
    <dbReference type="GeneID" id="13542256"/>
    <dbReference type="KEGG" id="pgr:PGTG_22563"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22563"/>
    <dbReference type="eggNOG" id="KOG1931">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004654_0_0_1"/>
    <dbReference type="InParanoid" id="H6QUZ7"/>
    <dbReference type="OrthoDB" id="10256906at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990071">
        <property type="term" value="C:TRAPPII protein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034498">
        <property type="term" value="P:early endosome to Golgi transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055505">
        <property type="entry name" value="DUF7077"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045126">
        <property type="entry name" value="TRAPPC10/Trs130"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022233">
        <property type="entry name" value="TRAPPC10/Trs130_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056913">
        <property type="entry name" value="TRAPPC10/Trs130_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13251">
        <property type="entry name" value="EPILEPSY HOLOPROSENCEPHALY CANDIDATE 1/TMEM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13251:SF3">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23274">
        <property type="entry name" value="DUF7077"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12584">
        <property type="entry name" value="TRAPPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23036">
        <property type="entry name" value="TRAPPC10_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="TRAPPC10/Trs130 N-terminal" evidence="5">
        <location>
            <begin position="53"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7077" evidence="6">
        <location>
            <begin position="768"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="domain" description="TRAPPC10/Trs130 C-terminal" evidence="4">
        <location>
            <begin position="1129"/>
            <end position="1225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF12584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF23036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF23274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS62610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1296" mass="145601" checksum="E14709F17476C943" modified="2012-04-18" version="1">MAGLLGGGDVNEVLLGLLDGHEGKKDSKNHPRLLTTTQKSMEPAQASTPPSSIVVSYHTSPSPDVWGRIQADLHRQLPLKNLHWKPKNRPLRVIPSVPISFQAFDSTQPASSASPPFVYLLFVACDDHEAYKTIIKQEIRTWLDSVQSNHSEWLLVHVSTAPKHNQSTAGIFRTKAGVLDKLKADFNPQKTERCIQLSYMSTMDSIVQASVHDSSTQWTELITRLKQSLLLVFEFKINEMEESLRTMVSTRSRQGWDFQSFFIQKAQLANQFEMMNLFEDGLIQYDELDAAFSQSLSDCPQIWHDKICGKSSSQYSRLALKTDASKEYLEVVKAGKMSIFDLRIYVFSKQINLLGRLSRLDEAIKRASVFISTFAAFLRSHEHKWPANYIESWVYCACNELVEWCEERITPGNKDTTTLDSLRCCAQLLELAHSQLDRIGIEVGHLPAIHPFNMSLGQPQEANSDQARSNLSPAITSPANLIDSLKDSKKFEKVYLDLARRTINLYQSTGRRRSALKLHCKTAAFEQTQARSESAHRLYSHLPAHYVDDKWTTIESSLLDRCASLQKELGLSREWLLSTLALVRSGVTYEPGKWSQDVMLRSATASQLDNADPVDQSALASKLMQDIREQSLKLEKDFAAIGFPIFSITLNSSVGRLSETFEGSVVKAKITNVLPCPIQVEAVRMKFSGRKSETLWFTARASQLLPGQTTIDLFCATPVAEILVLDVSQIRISRILFQYYHRPQGSQNEKTLDQEALEIVSFPKDLLGLDVKLELPPDIHLGEARKCLLSLFSGRKHLSHAAISISSDSDIRFEFDDSSLCVHPNTQVKMVNDSGKKITIETIQPESTVCLLIPFRTSTAQPLVEVNIIVDYRLSDDPSVKRTFRKSSRLKIAQPIEVKIREIFRPNWVFTKVTLATHRGYPLRIASARLELDEETSASQSLLSISPACGKSTSIVILPSQTSDHIFKIIPGAISATSSPSDSNFSQVIDSSRLKLVVKYRLIIDEIAECLRTRLMNGLEEDQKKAIDFLVKHVKDIMANEVDYDEQFYSTYLYDHVLHSGILMKKVDWSLVCERIEKDQAKKSQLVDRICAVIQDPSPVICPLDMPWQSITHPFNLSKSVILTTIEYQRVSGEEGQVGQVGRPMPIDMRISTSFKWTNLAPNSPPVGLVYELVGSEDQWLVGGRKRGTFFARDDSIEEITLILIPLRPGYLNLPRVTIKLTETKDGGSIRPTTPATSAPTTINNPGIERKNMQQDIVIQSSYSNALHPFLIVPSAAPSKFIIPPIRPYPTLKNLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3JPY0</accession>
    <name>E3JPY0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_00019</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307069.1">
        <property type="nucleotide sequence ID" value="XM_003307021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JPY0"/>
    <dbReference type="GeneID" id="10527360"/>
    <dbReference type="KEGG" id="pgr:PGTG_00019"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00019"/>
    <dbReference type="HOGENOM" id="CLU_047292_2_0_1"/>
    <dbReference type="InParanoid" id="E3JPY0"/>
    <dbReference type="OrthoDB" id="2506484at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP74063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="262" mass="29229" checksum="E78BA046317FA498" modified="2011-01-11" version="1">MSNSRRISHPMYSMGPFDIIELGPTTLKDHNNFGQFNYRSSVVLEEKTTGKHYNHEILLSGYGSRSTAPIRDRVYILGGRFVKNPEEPKNEADDEAECTGYTFFYDQALTLCIGRTEQYRTKVASLLMSKVGVFGFGVITNRQEVKVNGPNNSVQNDLHVSVKHTDYHTASKEVMEFQANYIVHGNRLLGHTFNLFQVGRKALIVGYISGHDDDAEVRQVTVLLVLISAGGQTTTVNLMSTPSASDSGQQPRPNLKRIGALW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQB3</accession>
    <name>E3JQB3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_00338</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5 6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74382.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307388.2">
        <property type="nucleotide sequence ID" value="XM_003307340.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQB3"/>
    <dbReference type="SMR" id="E3JQB3"/>
    <dbReference type="GeneID" id="10546652"/>
    <dbReference type="KEGG" id="pgr:PGTG_00338"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00338"/>
    <dbReference type="eggNOG" id="KOG0490">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_497085_0_0_1"/>
    <dbReference type="InParanoid" id="E3JQB3"/>
    <dbReference type="OrthoDB" id="6159439at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030154">
        <property type="term" value="P:cell differentiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.60:FF:000453">
        <property type="entry name" value="Unplaced genomic scaffold supercont1.84, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001356">
        <property type="entry name" value="HD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051000">
        <property type="entry name" value="Homeobox_DNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24324:SF5">
        <property type="entry name" value="HEMATOPOIETICALLY-EXPRESSED HOMEOBOX PROTEIN HHEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24324">
        <property type="entry name" value="HOMEOBOX PROTEIN HHEX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00046">
        <property type="entry name" value="Homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00389">
        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5 6" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="8">
        <location>
            <begin position="73"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
            <begin position="75"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="129"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="226"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="368"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="528"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="10"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="134"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="166"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="275"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="292"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="321"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="368"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="394"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="444"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="463"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="528"/>
            <end position="540"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP74382.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="567" mass="62188" checksum="49EDEEF2AF1BF1F0" modified="2012-04-18" version="2">MSSFGPSVVSPTQAISQQASSSPTSQANPNAEMSIYAHQKWNSSAQQPQLYLARLPTVTPASDVNAARSLQLGVPRPKRKRITPEQLEVLSNLFEYTDTPTFDLREAIGAKLGMSNREVQVWFQNRRAKVNRQRAPSNTQATLQDRPLPSTSQQDPSRASYGRQKSSSSELLLPSVSTMLKSSTDGSSSSFPSAPLLPPFQPTVPGGNNIPDPLVRRSVHDRCQLATRPSSPLDVSGSDRLPRSELQLRSPSGSHRRNNPSTPGSPYSRKWLPRRAPDASYHDHPADPLSPRRSSTDPQPKSSIIPDHLTVHHPARPRWSSHATRSRLNSQLTSQRPQSHYLNIASMRLDQQPHADAAVNLNSYSLTRTSLPTDENPTPQLIEPPAPSSLHDVSPNQESPNESNSSSRSSSPPSHHQSAQNRAPWQSRGESMPLNAPRPVFHNKAREDGQEHNRSNNHLGCPSQQSSSSSSTQARRSCSAEEPTLATSRSLFSSRCLPPLPNPVRPGPGSFPPPVALRVVAIKRDNSPINSAFSASPSSSEFRLPPLLNERHTDHLHIPPHNLSRTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQK2</accession>
    <name>E3JQK2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF659 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74307.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307313.2">
        <property type="nucleotide sequence ID" value="XM_003307265.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQK2"/>
    <dbReference type="GeneID" id="10546584"/>
    <dbReference type="KEGG" id="pgr:PGTG_00263"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00263"/>
    <dbReference type="HOGENOM" id="CLU_027159_0_0_1"/>
    <dbReference type="InParanoid" id="E3JQK2"/>
    <dbReference type="OrthoDB" id="2445862at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007021">
        <property type="entry name" value="DUF659"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052717">
        <property type="entry name" value="Vacuolar_transposase_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46169">
        <property type="entry name" value="DNA REPLICATION-RELATED ELEMENT FACTOR, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46169:SF29">
        <property type="entry name" value="DNA REPLICATION-RELATED ELEMENT FACTOR, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04937">
        <property type="entry name" value="DUF659"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF659" evidence="1">
        <location>
            <begin position="36"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP74307.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="547" mass="62297" checksum="BADEC049C84CF593" modified="2012-04-18" version="2">MISSAVPFSLLENPYFQQYQHEIAGPGFEIPRHGKMATHTLSKVHANLEAKTINRILDLDNITLSLDGWTDVAGNSVYALIVLKGPQSRFFIDVLYFHRQQHDARNIISSVKQSLSERCVDIEKACAIVTTSSPLMIEFQNLLRTEHSQILKIQCIQHAFNLMANDLLSNTMAHTIVEGNKTLVEYFVSNDYWSSYIEAWQKDHGIQPGLQTASGNRWFSISKVCEVVHLNRGGFEDCRDVSNDPSFNLPRLPEYILKLIEPNHFEANTSLIALLKPIVDGLRLLESPDMNLSDIWTVILTIYQSVLDASINPKNLQLKVHCIRMIHRQSAIFLDSIYIIGFFLHPVYRRVLKLSLEEITRMVLQVAKYWRFTRSDAISIREQLARYHSNLHPFTFRPESSPFDIKALPHWISVPDSPQTSTLRRFAITVFTIVPHASGVDGLRSIISKPGVQDKLSLVALGKLCQIRYHLLQDMKIHPPIVPTLGPDREHIKLDTAAATPVNHIDRMFDMKKWHQITTHTGSSPEVAEVNEDWELDDILPSFSSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQL2</accession>
    <name>E3JQL2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00354</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307404.1">
        <property type="nucleotide sequence ID" value="XM_003307356.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQL2"/>
    <dbReference type="GeneID" id="10527676"/>
    <dbReference type="KEGG" id="pgr:PGTG_00354"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00354"/>
    <dbReference type="HOGENOM" id="CLU_051119_2_0_1"/>
    <dbReference type="InParanoid" id="E3JQL2"/>
    <dbReference type="OMA" id="DESMWVI"/>
    <dbReference type="OrthoDB" id="2498930at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027532">
        <property type="entry name" value="Mdm12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204:SF1">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP74398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="288" mass="32759" checksum="380D96F459CB39FF" modified="2011-01-11" version="1">MSTEVIRETKVLLTPLNYALWLLPMEARLFRLKLLNIVKSEVEPDEKEKESWMKLNNDAYAEIIAYIGQEVLGFVSSALPVTPRFNGRGIWKLLKSQYAGDDLTSQTTALDQFLHLSFSSIATFIPAVRSGNQKISMSGLVLDDRVRTILMLKKLPSDYRSFRDIVSMNYGSESFESVIKKLESYVAQNDLDKKPTVSSTPLQSLLTRSNDQASASSTVCDHCKKPGHRVNNCWVKYPEKAPKSHTAHMTVQDNYNQLNDPTDFSHFRTADGKLHHVDEMRFEGVQYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQN4</accession>
    <name>E3JQN4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00639</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307689.1">
        <property type="nucleotide sequence ID" value="XM_003307641.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQN4"/>
    <dbReference type="GeneID" id="10528039"/>
    <dbReference type="KEGG" id="pgr:PGTG_00639"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00639"/>
    <dbReference type="HOGENOM" id="CLU_109921_0_1_1"/>
    <dbReference type="InParanoid" id="E3JQN4"/>
    <dbReference type="OrthoDB" id="2499896at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173111" evidence="1">
        <location>
            <begin position="21"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP74683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="217" mass="23973" checksum="81CC97FC47F15131" modified="2011-01-11" version="1">MVSLAYLITLLSVLVAVSRADEVTGAKGAEVEAKWYGNYYGSYSYSNMISWSGSYFNTFNLNPWINTWGNMYGPCFGGAAFPFGYSRFFAKATEAEAHSVSRRATRLDAEHLFRRESNEPDSVSCLSDKGDSQLFSKSECAKAAETLHREKAHSATSGNCRLSLFTEKDKVFAKDLPEKTLQDAVQSILKTCGQKSQSPGQKSHIDDKQAIMILSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRE4</accession>
    <name>E3JRE4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_00564</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00564"/>
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    <dbReference type="GO" id="GO:0005524">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="entry name" value="1-phosphatidylinositol 3-phosphate 5-kinase isoform X1"/>
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        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
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        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
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        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 3-PHOSPHATE 5-KINASE-RELATED"/>
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        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 3-PHOSPHATE 5-KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP74608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="300" mass="33610" checksum="FFA55CA4F7002875" modified="2011-01-11" version="1">MTPKMLGISEDWLRSKEFPEARYIHGVVFTKNIAHRKMKSECSVPKVLTIGMPIEFQRVDGYCKLDVLVSQERQYLKGLVHRLISLEPDLVLVDGNVSGVAIDYFVEAGVTVLRHVKSTVLQAAARSNKTPLISSLDKLLNLQVGQCDLFCVQTVHHRMIPNHHKKTFIRLEGCEPSLGGTLILRGGDLALLTKVKSLMCSMVGIIYSLRLEDSFLNDEGAIYSNDSTHETLLHQIRAIDLRTVLSRFRHAKKHICNFEPLKLPGKPGGAIPHSDLITLSILHINKVLSPPKEERDRALT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRQ9</accession>
    <name>E3JRQ9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00591</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307641.1">
        <property type="nucleotide sequence ID" value="XM_003307593.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JRQ9"/>
    <dbReference type="GeneID" id="10527497"/>
    <dbReference type="KEGG" id="pgr:PGTG_00591"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00591"/>
    <dbReference type="HOGENOM" id="CLU_280623_0_0_1"/>
    <dbReference type="InParanoid" id="E3JRQ9"/>
    <dbReference type="OMA" id="RIEMTYY"/>
    <dbReference type="OrthoDB" id="10020110at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="828"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="941"/>
            <end position="1119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="286"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="440"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="470"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="506"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="590"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="645"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="677"/>
            <end position="688"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
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            <begin position="736"/>
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        </location>
    </feature>
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            <begin position="757"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="958"/>
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        </location>
    </feature>
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        </location>
    </feature>
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            <begin position="1024"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1067"/>
            <end position="1080"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP74635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRV2</accession>
    <name>E3JRV2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_00508</name>
    </gene>
    <organism evidence="1 2">
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        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307558.1">
        <property type="nucleotide sequence ID" value="XM_003307510.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JRV2"/>
    <dbReference type="GeneID" id="10527810"/>
    <dbReference type="KEGG" id="pgr:PGTG_00508"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00508"/>
    <dbReference type="HOGENOM" id="CLU_2868681_0_0_1"/>
    <dbReference type="InParanoid" id="E3JRV2"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP74552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="64" mass="7229" checksum="F9034BD4D37B5070" modified="2011-01-11" version="1">MSKTDSVSETVTQTTRFLLTSSNYTIWILPMAAKLEDIGIRTYFTGVLKTDEKTPSEELAQLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>E3JS02</accession>
    <name>E3JS02_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small COPII coat GTPase SAR1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Small COPII coat GTPase sar1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">PGTG_01420</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasmic vesicle</location>
            <location evidence="2">COPII-coated vesicle membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Endoplasmic reticulum membrane</location>
            <topology evidence="3">Peripheral membrane protein</topology>
            <orientation evidence="3">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 21">Belongs to the small GTPase superfamily. SAR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP74827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319246.1">
        <property type="nucleotide sequence ID" value="XM_003319198.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JS02"/>
    <dbReference type="FunCoup" id="E3JS02">
        <property type="interactions" value="514"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JS02"/>
    <dbReference type="GeneID" id="10546944"/>
    <dbReference type="KEGG" id="pgr:PGTG_01420"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01420"/>
    <dbReference type="HOGENOM" id="CLU_040729_6_0_1"/>
    <dbReference type="InParanoid" id="E3JS02"/>
    <dbReference type="OMA" id="GLWNKHG"/>
    <dbReference type="OrthoDB" id="2011769at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030127">
        <property type="term" value="C:COPII vesicle coat"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070971">
        <property type="term" value="C:endoplasmic reticulum exit site"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061024">
        <property type="term" value="P:membrane organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003400">
        <property type="term" value="P:regulation of COPII vesicle coating"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016050">
        <property type="term" value="P:vesicle organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00879">
        <property type="entry name" value="Sar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000161">
        <property type="entry name" value="Small COPII coat GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006689">
        <property type="entry name" value="Small_GTPase_ARF/SAR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006687">
        <property type="entry name" value="Small_GTPase_SAR1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45684">
        <property type="entry name" value="RE74312P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00025">
        <property type="entry name" value="Arf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00328">
        <property type="entry name" value="SAR1GTPBP"/>
    </dbReference>
    <dbReference type="SMART" id="SM00177">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00178">
        <property type="entry name" value="SAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51417">
        <property type="entry name" value="ARF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51422">
        <property type="entry name" value="SAR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="10 21" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="11 21" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="13 21" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="14 19" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="17" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 18" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 21" id="KW-0653">Protein transport</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 21" id="KW-0813">Transport</keyword>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="27"/>
            <end position="34"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="20">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="129"/>
            <end position="132"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR606687-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR606687-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="PIRSR" id="PIRSR606689-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="RuleBase" id="RU003926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="EFP74827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="189" mass="21393" checksum="0786F2C053F93447" modified="2011-01-11" version="1">MFILNWFWDVLANLGLVNKNAKILFLGLDNAGKTTLLHMLKNDRLATLQPTLHPTSEELAIGNVKFTTYDLGGHQQARRLWKEYFPEVNGIVFLVDAQDPERFSESKIELDALLSIEELSKVPFLILGNKIDAPGAVSEEDLRHCLGLYQTTGKGKVPLIDIRPIEVFMCSIVMRQGYGDGFRWLAQYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>E3JU20</accession>
    <name>E3JU20_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">P-loop containing nucleoside triphosphate hydrolase protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_00876</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75545.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319964.2">
        <property type="nucleotide sequence ID" value="XM_003319916.2"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JU20"/>
    <dbReference type="GeneID" id="10543261"/>
    <dbReference type="KEGG" id="pgr:PGTG_00876"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00876"/>
    <dbReference type="eggNOG" id="KOG0054">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_27_6_1"/>
    <dbReference type="InParanoid" id="E3JU20"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18596">
        <property type="entry name" value="ABC_6TM_VMR1_D1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18604">
        <property type="entry name" value="ABC_6TM_VMR1_D2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03250">
        <property type="entry name" value="ABCC_MRP_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03244">
        <property type="entry name" value="ABCC_MRP_domain2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000825">
        <property type="entry name" value="ABC bile acid transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000630">
        <property type="entry name" value="ATP-binding cassette (ABC) transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000366">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050173">
        <property type="entry name" value="ABC_transporter_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223:SF353">
        <property type="entry name" value="ABC TRANSPORTER ATP-BINDING PROTEIN_PERMEASE VMR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24223">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="56"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="214"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="426"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="463"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="583"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="670"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1077"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1173"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1259"/>
            <end position="1292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1357"/>
            <end position="1377"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="426"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="780"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="13">
        <location>
            <begin position="1092"/>
            <end position="1396"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="1443"/>
            <end position="1702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="319"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="519"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1562"/>
            <end position="1584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EFP75545.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1737" mass="191975" checksum="AB555EDFFDC23457" modified="2012-04-18" version="2">MYFQSVIESQQLPLQDYGERQIRGDHHWCRGFSQPSWNGQDFSRCARLKYLIGEPALVLVLLLGGSFILPCLLCLWARIRSRKTAGSWLTRRVVPPYEALHNRGSPQATLHDPELNGPDAVSQAEDQIIAIEVMREGGLGIDKQDRQELHGVNSLPSPLHIHQDCCGETTTYLTALVKSWKDISISACTVGMLVTAAARYFISTDADAGSNWLLIPIILWLSVSVMSIVKLLIQIRTVIRNKPHPHPTYFELEYRTVPIYLVSLPLELLDTRSILLQHFGGQHTVEATYQLMVIRLLTLMFLIIANVLELVTPRPTTLLPGSSKERSSSIAPVDSQPEVQRPAPLEPGRSLLSLAYFIHTDSYIWRHKSVTATEESIPDIRADDKSAAILFRWKKEQNEYEAGNIKPSLLRALTWHSRNILLSQQLFAYFNAAGSLLPPFFLQRIVGFISSKSGENPQPGHVALLYAFGMLSTQAFLAFSLSASLMTGRRLSLRLRALLITLIFTKSLRRTGVNAKLSEGVDTRNDDSSTQIKSPSKEDPGGSASTGKITNLVSNDIASLAEIGSHLHFLWPESPVQLILAGIYLYVLLGYSALAGMFCIIIAVPIQSYLTAIWARYQDMLMGAADKRLGLAAEVINNIKVIKFFAWESKFLQKMQVLREAELTMLFKRLLVTIGESAVSLSVPIIVSIVTFYTHTKVFGKSLTAEEAFTALALFNVFKFPLNVLVDTISGVSQSYVSLKRIESFLNEKDTEKYSSLSVPRREAGDPLVGFKNATFTYEAKQEDADPTLESSTFKLGGLDFGFPEGKLSLIIGRVGSGKSTLLLSLLGETTKLSGEAFLPSPVCRAWGLDPELQLTDTTAYCPQQAWLLSDSIRNNILYGSTMNRSRYTQVLKACALAPDLATFTDGDLTEIGDKGTVLSGGQKARISLARALYSPAKVLLLDDVLSAVDSHTAQHLFNHVLNGPLMKDRTCILVTNAVDLCMPAASFFVSLDRGQVVYAGDPSASKLPSTFVSGNRKVNSNQSAPTIDPELIIEDLAAAILNKDEEIIELKTRATQTLVEEEHQAVGAVSFRTYKLYYDCLGGLIPLFNTFVMFILAELGNILSTWVLKQWTESNQKSASLTNLTSSSLLPPSILTATKILSTSPNPLYLNLHADSSSERDSIPEARPDHYILLYLLTGMFALAFELIRETYFTCQSIIAGRKLYERLIGTLLSAQVRFFDTVPMGRVLSRLSTDVRTVDQDLADTLIYMAEEVLGTMAILIVVIAVLPMGFLLCAMVACSIYVAIGYLYLASTREIKRYESTSRSPVISLCTECLGGVTSIRAYGDIGRYTQQMFRLIDGYNRPFFMLWMCNRWLSCRIDTAVALFTFLVVIYIIQSDMPAALSGFALSYVISLNKKTLLKLTTCALIVKVERIHEYLGIDQEPKAGIQPPAAWPSKQGAIEVQGLTARYAPHLPLALKSVSFSVKAGEKIGICGRTGSGKSTLALSFFRFIEAEAGQILIDGLDISKLDLTSLRSRLTIIPQESVLFSAPIRWNLDPFSEHDDNQIWDALRRVGMAAPSSELLPNGSNAADEPPEPSSMAQDLPFITSLDMEVKEGGKNFSTGQRQLLAIARAILKLENSALLILDESTASLDAESDEKIQATIRSEMGNSTILCIAHRLKTIIDYDKVLVLSDGEVLEFDEPWKLLLDDEDGEQEVGSVKKTSAFRELCLKSGHYDELKQCALKAKELRSKSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWF5</accession>
    <name>E3JWF5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02821</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76380.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320799.2">
        <property type="nucleotide sequence ID" value="XM_003320751.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JWF5"/>
    <dbReference type="GeneID" id="10534273"/>
    <dbReference type="KEGG" id="pgr:PGTG_02821"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02821"/>
    <dbReference type="HOGENOM" id="CLU_026798_1_0_1"/>
    <dbReference type="InParanoid" id="E3JWF5"/>
    <dbReference type="OrthoDB" id="2511868at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="1">
        <location>
            <begin position="144"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76380.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="229" mass="26526" checksum="78BFFAE95C94BE2C" modified="2012-04-18" version="2">MGSFCGNCVDLGALKNATGDRHRIRHVNRASKLANTRNKAKKRAELHAWIQEQVLNLSMGLFNSIQWQRLTWYPNTRLYPTVPFDKATAKPTRHPTPAEVQKACKFADRNFYLMNAGHPVLLDPRNPESVIAIMDFTPWNRLTKTDKANLEFVSTFLHQTKEFVNPVSSSTRSWGGKMWGIGWRKSQDILQIFGRYIKQFPPNKMEKYDTLFQKSKRSQLLFTPSHVHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWP0</accession>
    <name>E3JWP0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_02906</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic/archaeal RNase P protein component 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320884.1">
        <property type="nucleotide sequence ID" value="XM_003320836.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JWP0"/>
    <dbReference type="FunCoup" id="E3JWP0">
        <property type="interactions" value="276"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JWP0"/>
    <dbReference type="GeneID" id="10528236"/>
    <dbReference type="KEGG" id="pgr:PGTG_02906"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02906"/>
    <dbReference type="eggNOG" id="KOG4046">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078577_1_0_1"/>
    <dbReference type="InParanoid" id="E3JWP0"/>
    <dbReference type="OMA" id="IPKSECV"/>
    <dbReference type="OrthoDB" id="124041at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033204">
        <property type="term" value="F:ribonuclease P RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.210">
        <property type="entry name" value="Ribonuclease P/MRP, subunit p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00754">
        <property type="entry name" value="RNase_P_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016848">
        <property type="entry name" value="RNase_P/MRP_Rpp29-subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036980">
        <property type="entry name" value="RNase_P/MRP_Rpp29_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023538">
        <property type="entry name" value="RNP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023534">
        <property type="entry name" value="Rof/RNase_P-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002730">
        <property type="entry name" value="Rpp29/RNP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01868">
        <property type="entry name" value="RNase_P-MRP_p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00538">
        <property type="entry name" value="POP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101744">
        <property type="entry name" value="Rof/RNase P subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP76465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="301" mass="34243" checksum="E9EB38BDA4F26590" modified="2011-01-11" version="1">MENMNQTGTENASSSNEAVQDLYAEPKFIKKKRKKEVNEGIENPVWNSSTGSLPVKEIISTIVPEHIPHLEKNYQSKVNGRHLALHPTKRTQSRRNQFSGMKSKRRSATNRQVQFLELEKLIEAEQKRYRYQDFVPLHQLWLGYMAELLELRLKSLDDQNNIPTLPTEGPLSRSEKFAGMPAISAIQSKLLKADYHGAHLSVHKAKNPSLVDLQGIVIQESEQTFKLIQADNRIKVIPKAHTIFELSLPLLQAPTKVTGPSENNKSGDSSFLVFKIFGNQFIFRPSERINKKFKSKACSQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXI1</accession>
    <name>E3JXI1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_02217</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76756.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321175.2">
        <property type="nucleotide sequence ID" value="XM_003321127.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXI1"/>
    <dbReference type="STRING" id="418459.E3JXI1"/>
    <dbReference type="GeneID" id="10528777"/>
    <dbReference type="KEGG" id="pgr:PGTG_02217"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02217"/>
    <dbReference type="eggNOG" id="KOG1079">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_561547_0_0_1"/>
    <dbReference type="InParanoid" id="E3JXI1"/>
    <dbReference type="OrthoDB" id="6141102at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035098">
        <property type="term" value="C:ESC/E(Z) complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046976">
        <property type="term" value="F:histone H3K27 methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006560">
        <property type="entry name" value="AWS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026489">
        <property type="entry name" value="CXC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045318">
        <property type="entry name" value="EZH1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45747">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE E(Z)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45747:SF4">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE E(Z)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51215">
        <property type="entry name" value="AWS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51633">
        <property type="entry name" value="CXC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CXC" evidence="11">
        <location>
            <begin position="338"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="AWS" evidence="10">
        <location>
            <begin position="393"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="domain" description="SET" evidence="9">
        <location>
            <begin position="443"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="16"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP76756.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="569" mass="64662" checksum="2341CC723D4E9780" modified="2012-04-18" version="2">MANSDLNLHEDDPEINPSSSELKSSNKQTNLSQSDPLPPLDGPTAFKELKGQQIAEALSSIRVGLEEDRLNPTLNAPPWLQILSNLSDTSGSRCEPNFPQNKAAPVFEHHIRPPQYTWCGLLDRNAYPKRDNIRSHESNELTAIRFVPYFEAYDKQSPAEKLKELEETASTEKYEICLTDRICPDVLRQNFYELVEAFNLPSNECLLGIMSPLPPNGITEPPSDDQLIQSLALKISGNDEAKFSRLYDIYSRDWTRLKKSSRLKNTPIPLRIISSEFSIAEIIDSFRLTLPANKRIFKTSASDSSNWSKLCHICQQFYCEGHYDQRVPGKPRHHSPNWRSPSPDRYLNALPVDTGGYVPCSHSGKCFANQDCDCYKLTTYCDRFCQCAESCPRRYQGCRCRRICDEDCECVQRNRECDPLLCKCPTCKPTNPCSNFMFAKKKKRTFVAPSKISGFGLFAGEAISMGDYIGEYTGVLLRSDELAKQSMLTSLSKSSYLFDLNQFDTVDSGKFGNRTRFINEPINKRYNADPCVWYVAGTQVLRIFASKKIRVGEEITINYGESYHARDWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXP3</accession>
    <name>E3JXP3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_02279</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76818.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321237.2">
        <property type="nucleotide sequence ID" value="XM_003321189.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXP3"/>
    <dbReference type="FunCoup" id="E3JXP3">
        <property type="interactions" value="392"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JXP3"/>
    <dbReference type="GeneID" id="10528921"/>
    <dbReference type="KEGG" id="pgr:PGTG_02279"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02279"/>
    <dbReference type="eggNOG" id="KOG0417">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1152255_0_0_1"/>
    <dbReference type="InParanoid" id="E3JXP3"/>
    <dbReference type="OrthoDB" id="7851174at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23792">
        <property type="entry name" value="UBCc_UBE2D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000010">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2-16 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="8">
        <location>
            <begin position="95"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP76818.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="241" mass="26788" checksum="384D17CB43AC270D" modified="2012-04-18" version="2">MVGRGNEVVDRLVIDTSTPLGISSKTWCPWPSFNTVSCNGPLDNFPLIKAAVSVCIPHTKPSVDFNLQALNQSTPTEPNKSNNSFFSFVFFSFLMALKRINKELHDLGRDPPSSCSAGPKGDNMFQWQATIMGPGDSPYAGGVFFLMITFPTDYPFKPPKVCFKTKIYHPNINSNGSICLDILREQWSPALTISKVLLSICSMLTDPNPDDPLVPEIANLYKTDRAKYEATAREHTRKYAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3JY83</accession>
    <name>E3JY83_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_02469</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP77008.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321427.2">
        <property type="nucleotide sequence ID" value="XM_003321379.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JY83"/>
    <dbReference type="GeneID" id="10529275"/>
    <dbReference type="KEGG" id="pgr:PGTG_02469"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02469"/>
    <dbReference type="eggNOG" id="ENOG502R9HI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_566364_0_0_1"/>
    <dbReference type="InParanoid" id="E3JY83"/>
    <dbReference type="OrthoDB" id="2507983at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022698">
        <property type="entry name" value="OrsD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12013">
        <property type="entry name" value="OrsD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="18"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP77008.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="406" mass="45059" checksum="36C11063EAF03C27" modified="2012-04-18" version="2">MQQPTSSPVNTHPPSSQHQCLKDGCMEVFANFGELRKHIRNVHQHSATCMTHSKKQVTIFRSSEGKFGCPNKGCDYTAGPPINIQNHLKLCDPEALNPPRAMPIPPTGSVTVVPLGDQVMAIPDCPHLAYNNYAKTLICVTCHEGIPTSNVHGHMLSSHKLKVAKAEIIDKFLAYGIKLPEDNIKYPPRKNRVDPCIPVQGLYMYLGYTCTALYHGKPCGLSGCKEKTLSNHLREQHQVSGSGWKDYCETRFVQHLRTDHIKFFSVLYRQPSPEIICSSHLGSGNKIQAEGSILWDKLSQELSVLDVLGVKTRSGMKKDHASLVTAYLKSSGIYEHIELCKELVGCADSADLYKEINVSQKSAALVSFVDAWMACRMSKIDDTHFGLRKLAIKESIKLPSHKALTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3JYD2</accession>
    <name>E3JYD2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">TdcF protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03013</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RutC family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321476.1">
        <property type="nucleotide sequence ID" value="XM_003321428.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JYD2"/>
    <dbReference type="FunCoup" id="E3JYD2">
        <property type="interactions" value="236"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JYD2"/>
    <dbReference type="GeneID" id="10529404"/>
    <dbReference type="KEGG" id="pgr:PGTG_03013"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03013"/>
    <dbReference type="HOGENOM" id="CLU_100715_7_0_1"/>
    <dbReference type="InParanoid" id="E3JYD2"/>
    <dbReference type="OMA" id="GSYFKEP"/>
    <dbReference type="OrthoDB" id="309640at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019239">
        <property type="term" value="F:deaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00448">
        <property type="entry name" value="YjgF_YER057c_UK114_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.40:FF:000001">
        <property type="entry name" value="L-PSP family endoribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="entry name" value="RutC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006056">
        <property type="entry name" value="RidA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019897">
        <property type="entry name" value="RidA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00004">
        <property type="entry name" value="Rid family detoxifying hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803">
        <property type="entry name" value="2-IMINOBUTANOATE/2-IMINOPROPANOATE DEAMINASE RIDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11803:SF58">
        <property type="entry name" value="PROTEIN HMF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="YjgF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01094">
        <property type="entry name" value="UPF0076"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="198" mass="21479" checksum="FF0E072B5E2ACF04" modified="2011-01-11" version="1">MVKSNPGLSALRRAVFQNLNAPLRRYQALVIGRPGLPASIFDKPTYPSRTPYRSLTNTRPLSNTMSAPISLNTINGTPGAPEAIGPYSQAVVTSNGLIYSSGSIPLDPSTMKVIHPTDVELQTEQCLKNLSKVLEQAGGSIQSVIKVTVFIKDMAQFSKINEVYAKFFKDHKPARSCVEVARLPLDVLVEIEAVAAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZ71</accession>
    <name>E3JZ71_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_03302</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321765.1">
        <property type="nucleotide sequence ID" value="XM_003321717.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZ71"/>
    <dbReference type="GeneID" id="10538053"/>
    <dbReference type="KEGG" id="pgr:PGTG_03302"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03302"/>
    <dbReference type="HOGENOM" id="CLU_1190382_0_0_1"/>
    <dbReference type="InParanoid" id="E3JZ71"/>
    <dbReference type="OrthoDB" id="10364312at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP77346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="233" mass="26174" checksum="9B4989B665867096" modified="2011-01-11" version="1">MDDTGLDVVGRLSRVAWLDRSRSFICKGRGKTKPALMYGYIQHLSTSFQIQQPPTVSGHPPNKKSCPPTTIFKQTQKTNNQHAKKAEYGYHPRRPPFIPAPRSRSAPPQHCGNGFVQNLKGDPTPLCTNYEDKRFKCGAESCHIYDAQGKQQPYSTLTFHHCQRIGDNGEVKASDQTIQPTRFEADNKAKTLSATGTETGVQGEGYYKCSWNNDKDPNANRPWCHRCNAILYT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZH4</accession>
    <name>E3JZH4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_03405</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321868.1">
        <property type="nucleotide sequence ID" value="XM_003321820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZH4"/>
    <dbReference type="GeneID" id="10538327"/>
    <dbReference type="KEGG" id="pgr:PGTG_03405"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03405"/>
    <dbReference type="HOGENOM" id="CLU_143010_0_0_1"/>
    <dbReference type="InParanoid" id="E3JZH4"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP77449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="155" mass="17826" checksum="7969A9B4F107D74C" modified="2011-01-11" version="1">MTHQSPIYVSSNGSSGVGPMADELEISMLLTPHGHTFYSDDVFSQDEFDSLEAVVLNRFVHYCKTNQPKDYNDHDARVHTYIRIREAFYQANNHKLEKSVPNGMPHSHHFNTPKKFDMTGGIFSSQRRSVQFFQRVGSSRILVHNPVRPVPFIFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZI0</accession>
    <name>E3JZI0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="15">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PGTG_03411</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 15">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="15"/>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77455.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321874.2">
        <property type="nucleotide sequence ID" value="XM_003321826.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZI0"/>
    <dbReference type="FunCoup" id="E3JZI0">
        <property type="interactions" value="442"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JZI0"/>
    <dbReference type="GeneID" id="10541041"/>
    <dbReference type="KEGG" id="pgr:PGTG_03411"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03411"/>
    <dbReference type="eggNOG" id="KOG0214">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000524_5_2_1"/>
    <dbReference type="InParanoid" id="E3JZI0"/>
    <dbReference type="OrthoDB" id="10248617at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005721">
        <property type="term" value="C:pericentric heterochromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003968">
        <property type="term" value="F:RNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140727">
        <property type="term" value="P:siRNA-mediated pericentric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.150:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1070.20:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1100.10:FF:000005">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1110.10:FF:000003">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1800.10:FF:000002">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1800.10">
        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="entry name" value="RNA polymerase Rpb2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007646">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007647">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007175">
        <property type="entry name" value="PRK09606.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04566">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04567">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15 23" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 15" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="19">
        <location>
            <begin position="1"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="18">
        <location>
            <begin position="121"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="20">
        <location>
            <begin position="376"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="21">
        <location>
            <begin position="474"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="22">
        <location>
            <begin position="575"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="16">
        <location>
            <begin position="652"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="17">
        <location>
            <begin position="1026"/>
            <end position="1116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF04566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF04567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="EFP77455.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1131" mass="127385" checksum="E64781A4E3D23A35" modified="2012-04-18" version="2">MTESDGTVSPMFPNEARLRNLTYSSPLYVDMSKSNKLATGEFDEETGEPIYIADDVDNDEKNSTQIYIGKVPIMLRSEFCILDKLEDPGLYELNECPFDQGGYFVINGSEKVLIAQERMAANHVYVFAKAGPLAVEKGSKTVSSMQVKMYGGHKAEKAGGGATIKTTLPYIRNDIPIVVVFRALGIIPDKDILEHICYDRNDTAMFEMLKPCLEDSFPIQEQEVALDFIGRRGTATGLSREKRLKYAEEILQKEMLPHISMSEGQQGKKAYFFGYMIHRLLLAALDRRDLDDRDHFGKKRLDLAGPLLAGLFRMLFRKLTKDVYRHLQKCVEMQKPFNLNAAVKSNTITNGLKYSLATGNWGDQKKAMQARAGVSQVLNRYTFASTLSHLRRCNTPIGRDGKIAKPRQLHNSHWGMVCPAETPEGQACGLVKNLALMSYISVGSPSAPIVEFLEEWGMESLDEFSSDMSNGTKVFVNGVWQGVHRAPAELLDTIKRLRRCGDIEPEVSVMRDVRERELRVFTDGGRVCRPLFIVENQQLLLKKEHIEWLSNGCETAEEDQDAGEEAADDGVPFGWSQLVARGIVEYLDAEEEETVMICMTPEELEQSREYQETGQVPKENYDPAARLKGNTSMYSHTWTHCEIHPAMILGICASIIPFPDHNQSPRNTYQSAMGKQAMGVYLTNFRTRMDTMANILYYPQKPLATTRSMEYLRFRELPAGQNAIVAILCYSGYNQEDSVIMNQSSIDRGLFRSFYYRSYMDQEKRAGALQMEEFEKPTRDSTLRLKHGTYDKLDDDGIITPGTRVSGDDIIIGKTAPIPKDSEELGQRMKTHTKRDVSMPLKSTENGIIDQVLVTTNQDGLKFVKVRIRSTRIPETGDKFASRHGQKGTVGILYRQEDMPFTSEGVCPDIIINPHAIPSRMTIGHLVECLLSKVSTITGNEGDATPFSEVTVEAISALLKDKGYQSRGLEIMFNGHTGKKLRAQCYLGPTYYQRLKHMVNDKIHSRARGPVQILTRQPVEGRSRDGGLRFGEMERDCMISHGIAGFLKERMFDVSDAYRVHVCDFCGMVAIANLKKQSFECRSCRNKTAISQVYIPYAAKLLFQELQSMNIASRFVFESAGEHVSRGDMIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3K0W5</accession>
    <name>E3K0W5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_03896</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP77940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322359.1">
        <property type="nucleotide sequence ID" value="XM_003322311.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K0W5"/>
    <dbReference type="FunCoup" id="E3K0W5">
        <property type="interactions" value="116"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K0W5"/>
    <dbReference type="GeneID" id="10541088"/>
    <dbReference type="KEGG" id="pgr:PGTG_03896"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03896"/>
    <dbReference type="HOGENOM" id="CLU_041939_2_1_1"/>
    <dbReference type="InParanoid" id="E3K0W5"/>
    <dbReference type="OMA" id="ISHMKDE"/>
    <dbReference type="OrthoDB" id="341421at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046975">
        <property type="term" value="F:histone H3K36 methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042800">
        <property type="term" value="F:histone H3K4 methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050600">
        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF47">
        <property type="entry name" value="ACTIN-HISTIDINE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SET" evidence="4">
        <location>
            <begin position="26"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP77940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="470" mass="53017" checksum="5E7327096323D03C" modified="2011-01-11" version="1">MTQQTSQEWIDLLEWLQRNETNTGEFKATMRSSTSKGRSLVATEDIEESTSILCLPRSVLLDTKTIPQEFVEKHCLELTSTQALSLFLTVYRSSRRNCADPDRFEYPFGCYLSTLPDDLSGIPLVWQLRAIFGKMDEIDPTDLAQLDRLQTHVRPAPPPTDSINPFDFYQLMTPTTINQAEEVLLRFIDDWAALVPTLRDCYPHARLWDLLWAWLIVNTRCVSFNIGAKKMADNISLAPAFDMANHSPISRVTAVANEQTLTMYSTQPAGLGPANPALNFPHSSPSDQPCMIRKGQEITFSYGPHANSTLLTEYGFVASEPNPWDSIDVTDHIVALFNRSDPTHLKKIELLKNTDYWEDYTIQCDPLESSHRNLVALRLLHTPEGRLSEWEAHIDGLADELSRDIEQAVRRTLDELFDQLQAKSRALVQKLEGPDICFAKFGLSVYSAQCLVTLAANELKALSSYSPSTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3K148</accession>
    <name>E3K148_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_03979</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP78023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322442.1">
        <property type="nucleotide sequence ID" value="XM_003322394.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K148"/>
    <dbReference type="STRING" id="418459.E3K148"/>
    <dbReference type="GeneID" id="10541332"/>
    <dbReference type="KEGG" id="pgr:PGTG_03979"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03979"/>
    <dbReference type="HOGENOM" id="CLU_011407_4_1_1"/>
    <dbReference type="InParanoid" id="E3K148"/>
    <dbReference type="OMA" id="NAIYMAN"/>
    <dbReference type="OrthoDB" id="2505730at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040521">
        <property type="entry name" value="KDZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18758">
        <property type="entry name" value="KDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="600"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="332"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="626"/>
            <end position="636"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP78023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="636" mass="73946" checksum="50C7DD157C615C20" modified="2011-01-11" version="1">MRAVWAAWRCPACFGPSPPNLNEYPWMSQDKLIICLDGNFQHHHHSKASRDYNQIRTPNMFVSQKEVDEMKAKIIDIELRQKPKDKRDQCTEAHKAADNKRNESSWKGCDDTGLMGCFCHHDAAIYMANINKSGEQCCFPMALLNKILNNESPHRHVGILYDIGCSLDKYMALRGLLSDQQDRLQFATSIFHAYVHSWTCQLDYNPRFNKGWGLSDREGLERMWSYLSPLVRPLRYATQNHWLAAISHQLKYHNTRGIKNLHKPNPFSTTGQNFTKRFFKAQWEEQRKFKETHTDEREENRDKLVEVYEQEAALEALRTWLRNTPSTYLASKQEAHELLDELETTAEELRKAAESLGRDHQTKPDEAKEEQKLLLLLWEAKSELFVQAVELWAEKQPLTDSRTIRRRLGTKLSEKIQKAIQKRWGPINKLIATFNTRFAQYVAKFPRQRVADADEHPLTYKTFESMAIDHKFWNDGIYYHSKAPWAIDLVGVMALDMNMEPPEDHIDRMELGNYDCREKLRIINKELQTRLFDHGVLIQEWTGTITWMCAHCRPELLRDIYSDWNDIVQHVATNQTSRQPKKNKEEPVVDNVEEEAVVGIQVDDGKEVTSGEAANSAGEPARSDWEDVDDAQDACD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2C5</accession>
    <name>E3K2C5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">FACT complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_04450</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the FACT complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M24 family. SPT16 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78494.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322913.2">
        <property type="nucleotide sequence ID" value="XM_003322865.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2C5"/>
    <dbReference type="STRING" id="418459.E3K2C5"/>
    <dbReference type="GeneID" id="10536175"/>
    <dbReference type="KEGG" id="pgr:PGTG_04450"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04450"/>
    <dbReference type="eggNOG" id="KOG1189">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_433553_0_0_1"/>
    <dbReference type="InParanoid" id="E3K2C5"/>
    <dbReference type="OrthoDB" id="2496911at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035101">
        <property type="term" value="C:FACT complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031491">
        <property type="term" value="F:nucleosome binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.230.10:FF:000005">
        <property type="entry name" value="FACT complex subunit spt16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.350.10">
        <property type="entry name" value="Creatinase/prolidase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029149">
        <property type="entry name" value="Creatin/AminoP/Spt16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029148">
        <property type="entry name" value="FACT-SPT16_Nlobe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040258">
        <property type="entry name" value="Spt16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13980">
        <property type="entry name" value="CDC68 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13980:SF15">
        <property type="entry name" value="FACT COMPLEX SUBUNIT SPT16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14826">
        <property type="entry name" value="FACT-Spt16_Nlob"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01285">
        <property type="entry name" value="FACT-Spt16_Nlob"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0158">Chromosome</keyword>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="1" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="domain" description="FACT complex subunit SPT16 N-terminal lobe" evidence="4">
        <location>
            <begin position="11"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="455"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="486"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="597"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="597"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="617"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU367052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP78494.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="630" mass="70686" checksum="D36BAB3BAA868FBE" modified="2012-04-18" version="2">MWPNSRVEGGLDVQFFHSRIESFIKSWKSADGPETGDLQSCGGILMGEGSLRSGNRPLHVFLFGDMLIGSLIFITPTTVTFFCSTRQAEILTPLTKLHCNEFDSSDGLNIDVRVIVRPPDPNKGDSWMRHLLACVEAVISNSERIGRMSQDKSLAGWLAFLKSEGKYALYQEAAVISDEVSVILAIQHPQEIKLTEIACQMSHQLMSCLFDQIISLVKSDRDITNEEVGQLIRAKHKNGNMWEGATFEPNFDRGDACLRIFPVVRSNGKYSFRKSDPHAAERLGNTGIFLTGLGIQYKSYCSYIRRTLMVDPHPTQQDNYSYLLELRGFALGQLKEGVTGHQVYASIKRKVKVDRPGIRLPKSFGSSLGVDPHNPLLNLNRNCFSVLKRNMVFSLSLGFLNIEDPFDAEKTYSLHIADTVRIGKTRAKILCDGLNLSSEITFFLNTDPLEFDRVKGEDVGHDSGEEESIEGSDILSKRSGSFDEEWDNAKRQKLAPAGAPSSDEPDCMYTHSESDVDIKPRVTNEDSVARNLVGVVNAQGQARRTIKMEARDVDQRLDVLKHAEESMMKGLGDIENKLNDFKTQVKQNFAELRKWAATDNRTEPERRTLKIEEESTTKNSETFQETGKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2P4</accession>
    <name>E3K2P4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04569</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323032.1">
        <property type="nucleotide sequence ID" value="XM_003322984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2P4"/>
    <dbReference type="GeneID" id="10536465"/>
    <dbReference type="KEGG" id="pgr:PGTG_04569"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04569"/>
    <dbReference type="HOGENOM" id="CLU_2074313_0_0_1"/>
    <dbReference type="InParanoid" id="E3K2P4"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="118" mass="13180" checksum="194B0C69C2EDA829" modified="2011-01-11" version="1">MPGGLRLADALRVQVKLVGSHERGKKVLFDGDIVSNTQSPDNTANERYEPTEAGKRPTFWESLDLLGSMVGTLDFYPLCNSAKPAIEISQQLKEDSNEQSCTFVSYSYALRYSSWMPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2V7</accession>
    <name>E3K2V7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_04632</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78676.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323095.2">
        <property type="nucleotide sequence ID" value="XM_003323047.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2V7"/>
    <dbReference type="GeneID" id="10536611"/>
    <dbReference type="KEGG" id="pgr:PGTG_04632"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04632"/>
    <dbReference type="HOGENOM" id="CLU_2432983_0_0_1"/>
    <dbReference type="InParanoid" id="E3K2V7"/>
    <dbReference type="OrthoDB" id="447953at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP78676.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="91" mass="10278" checksum="0236D50532353365" modified="2012-04-18" version="2" fragment="single">RRKPSPDQSALEVALNNLDKCLDKCREPPQGALEAYETFAQPLVQDYGQLLEAFGPSFALAKRVKIHPALLPSNALLKSNSSHQQYWYQCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3K4H3</accession>
    <name>E3K4H3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_05480</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79159.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323578.2">
        <property type="nucleotide sequence ID" value="XM_003323530.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K4H3"/>
    <dbReference type="STRING" id="418459.E3K4H3"/>
    <dbReference type="GeneID" id="10530982"/>
    <dbReference type="KEGG" id="pgr:PGTG_05480"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05480"/>
    <dbReference type="HOGENOM" id="CLU_831914_0_0_1"/>
    <dbReference type="InParanoid" id="E3K4H3"/>
    <dbReference type="OrthoDB" id="2512003at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172385" evidence="2">
        <location>
            <begin position="26"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP79159.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="270" mass="27394" checksum="17483B670DA429BF" modified="2012-04-18" version="2">MVSLRLAQLVAIVVCMYACTDTLQAQATTGALKLNEASSKAPPQLGINGGNRTTGVNNTASKTNQAPVMPGANQPGQNPSKPTNPSPNQKTPQATLKPKAATGAVRPGKNASQSEEDRKPAGAEGLVRREVYSSRKILWKRSDLPNTTGTGNKPTSPQEDNSDHIKSGDKNHSSKGNVDKKLDGFSGGLKKRKMMSKMGGGGGGLKSKMGGGGSSGGGGGLKSMMGGLKSMMGGEGGGGGLKSMMEGISGAFGGLGDMVSLMGMMGEEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3K4R6</accession>
    <name>E3K4R6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">hAT-like transposase RNase-H fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05552</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323650.1">
        <property type="nucleotide sequence ID" value="XM_003323602.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K4R6"/>
    <dbReference type="GeneID" id="10531055"/>
    <dbReference type="KEGG" id="pgr:PGTG_05552"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05552"/>
    <dbReference type="HOGENOM" id="CLU_009635_1_0_1"/>
    <dbReference type="InParanoid" id="E3K4R6"/>
    <dbReference type="OMA" id="NWKEIDN"/>
    <dbReference type="OrthoDB" id="2508980at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF5">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="250" mass="27923" checksum="3F2F19E38780D2A1" modified="2011-01-11" version="1">MAKQICNLLVGNKGYQPGSWDPTSMHIRCFCHKLALIVNAGLQALSLKTLPPKREKASVLGFFPVLGKMVEEDEQDLSVQDLPVDVKGAKKTSPPINLALDSDSDYGNADNENSDKGPKESDENNTDHETTSAAHLQHAKTTKLKELTKELDVVIKQITRSAAQRSNFDLTTRKLNFKVAPLIAGYGIRWNICYQSHCKAIEAREVIDQILKDNQQHNSPEIFSNAYFSPRDWKELDDLNCELECEPGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3K5Y4</accession>
    <name>E3K5Y4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_05876</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323974.1">
        <property type="nucleotide sequence ID" value="XM_003323926.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K5Y4"/>
    <dbReference type="GeneID" id="10531385"/>
    <dbReference type="KEGG" id="pgr:PGTG_05876"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05876"/>
    <dbReference type="HOGENOM" id="CLU_1134045_0_0_1"/>
    <dbReference type="InParanoid" id="E3K5Y4"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP79555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="245" mass="26076" checksum="B5F4CA4AC94B554D" modified="2011-01-11" version="1">MADTVVRLIPPLLGGLGVAGGGGGAAGDGAVVGLEQVGWESSVWSKSAGSRWRRRRSRRGLGVAGGGGGAAGDGAVVGLEQVGWESLEEEEEPPATEPSSVWSKLAGSGWRRRRMEHIDQTILAVDKLLAHHDAAIHQEVVIQNGSVDCGMKRVEQADPVAEFEEKVLKRTVASLVILLEEERGPMVVVEEEEEDGLRNTFKISARRLALLDSSSISRIKKARRQIEGERATLRGDVESCGGGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3K643</accession>
    <name>E3K643_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-chaperonin molecular chaperone ATPase</fullName>
            <ecNumber evidence="2">3.6.4.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_05935</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the heat shock protein 70 family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.10" evidence="2"/>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324033.1">
        <property type="nucleotide sequence ID" value="XM_003323985.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K643"/>
    <dbReference type="FunCoup" id="E3K643">
        <property type="interactions" value="304"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K643"/>
    <dbReference type="GeneID" id="10531449"/>
    <dbReference type="KEGG" id="pgr:PGTG_05935"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05935"/>
    <dbReference type="eggNOG" id="KOG0101">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005965_3_0_1"/>
    <dbReference type="InParanoid" id="E3K643"/>
    <dbReference type="OMA" id="NIPPMQA"/>
    <dbReference type="OrthoDB" id="2401965at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031072">
        <property type="term" value="F:heat shock protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000002">
        <property type="entry name" value="Heat shock 70 kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000172">
        <property type="entry name" value="Heat shock 70 kDa protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.10:FF:000014">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000026">
        <property type="entry name" value="Heat shock protein 70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.34.10:FF:000004">
        <property type="entry name" value="Heat shock protein SSB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.30:FF:000005">
        <property type="entry name" value="Heat shock protein ssb1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.34.10">
        <property type="entry name" value="Substrate Binding Domain Of DNAk, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018181">
        <property type="entry name" value="Heat_shock_70_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029048">
        <property type="entry name" value="HSP70_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029047">
        <property type="entry name" value="HSP70_peptide-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013126">
        <property type="entry name" value="Hsp_70_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001413">
        <property type="entry name" value="PRK00290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19375">
        <property type="entry name" value="HEAT SHOCK PROTEIN 70KDA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00012">
        <property type="entry name" value="HSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00301">
        <property type="entry name" value="HEATSHOCK70"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100934">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100920">
        <property type="entry name" value="Heat shock protein 70kD (HSP70), peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00297">
        <property type="entry name" value="HSP70_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00329">
        <property type="entry name" value="HSP70_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01036">
        <property type="entry name" value="HSP70_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0346">Stress response</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP79614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="635" mass="68959" checksum="6EE9B1F0B05EF286" modified="2011-01-11" version="1">MSAEQTPTVDGNVPGAEAESTEFNGAIGIDLGTTYSCVGVWQNDRVEIIANDQGNRTTPSYVAFTAEERLIGDAAKNQAAMNPRMTVFDAKRLIGRRFDDSDVKKDMVHWPFAVIEKDGNPFIQVDYLGETKTFSPQEISAMVLAKLKETAETKLGKEVKKAVITVPAYFNDSQRLSTKDAGAIAGLDVLRIINEPTAAAIAYGLGEASDKKEKKERTVLIFDLGGGTFDVSLLSIAGGVFTVKATAGDTHLGGEDFDNALLEHFKNEFKRKTKLDIDGDARALRRLRSACERAKRTLSSVTQTTVEVDSLFQGTDFSSAISRARFEEINAQTFRSTLDPVDKVLKDAKIDKSKVEDIVLVGGSTRIPKIQSLVSDFFGGRQLNKSINPDEAVAYGAAVQAAVLTGQTSEKTADLLLLDVAPLSLGVAMQGDVFGVVVPRNTPIPTNKSRTFTTVEDNQSTVTFPVYEGERVTCKENRLLGEFELSGITPQPRGQAELVCTFEVDANGLLKVSALDRTSGRRANITITNSVGRLSSAEIDQMIKDAESFKQADKEFSAKHEAKQELESYISQVEATITSPEVGMKIKRNNKAAVEAELAKALEKLEIEDSSADELKKCQLGLKRAMQKAMAGTAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>E3K652</accession>
    <name>E3K652_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_05944</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324042.1">
        <property type="nucleotide sequence ID" value="XM_003323994.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K652"/>
    <dbReference type="GeneID" id="10531458"/>
    <dbReference type="KEGG" id="pgr:PGTG_05944"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05944"/>
    <dbReference type="HOGENOM" id="CLU_2098027_0_0_1"/>
    <dbReference type="InParanoid" id="E3K652"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP79623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="116" mass="11915" checksum="8ACB3A7DFE637922" modified="2011-01-11" version="1">MARLCSDANVVNGDDNAGLRLGRRVDYSLLRGREGGGGGGPGWLGFGETATRESWAVGRAVDEVQDTLGIPVTGWLIKAMHVAEMLGVELVEGGVLAATTDSSPPSPSGFVPFSSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3K667</accession>
    <name>E3K667_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05959</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324057.1">
        <property type="nucleotide sequence ID" value="XM_003324009.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K667"/>
    <dbReference type="GeneID" id="10532836"/>
    <dbReference type="KEGG" id="pgr:PGTG_05959"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05959"/>
    <dbReference type="HOGENOM" id="CLU_108601_1_0_1"/>
    <dbReference type="InParanoid" id="E3K667"/>
    <dbReference type="OrthoDB" id="10273221at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172408" evidence="1">
        <location>
            <begin position="23"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="213" mass="24718" checksum="683DE8CE7A761B43" modified="2011-01-11" version="1">MQLPSALTLTTLLSSFAMSARAVYPPPNDYIMSMGQDWANGYLSVYNSKGEDAFRWFKDYHKIRGSTQTTLADGSSRPLFSLDSSTDSCHYNKRFLQYKGQAWAERDYRLQLRDSKADMWHFNYPDKSGIRRYYRFNKNSSNMGGRIYRVARGQKDVLEGLLRTNVRYDKWWNDPKGRNTFTLSIVDEAPVPEMVMLMALVLEHSISCRGMQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K6N6</accession>
    <name>E3K6N6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_05257</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EFP80032.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324451.2">
        <property type="nucleotide sequence ID" value="XM_003324403.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K6N6"/>
    <dbReference type="GeneID" id="10534049"/>
    <dbReference type="KEGG" id="pgr:PGTG_05257"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05257"/>
    <dbReference type="HOGENOM" id="CLU_1289491_0_0_1"/>
    <dbReference type="InParanoid" id="E3K6N6"/>
    <dbReference type="OrthoDB" id="10505854at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP80032.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="206" mass="22978" checksum="A9E1FEA24E155087" modified="2012-04-18" version="2">MSRFGLDLLGRENPPPGYYSASKYWRVQGVGDQPVNLMVQMVDCHTSYKLFSPKDASKPQGAWNMIKSKQQFLISFAAFKGTEAAIQNAIRFGSPHIDWKISMKLPAADEFLKPAKYTVNDKSSYSHWIATIVDNGKDHTDVVLEVAMVNPASLKKDEKLAIELNKHNLKQAAVQQASGSNPSVKASKFDPINILTNKIYATHPLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3K7G1</accession>
    <name>E3K7G1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_06241</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80285.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324704.2">
        <property type="nucleotide sequence ID" value="XM_003324656.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K7G1"/>
    <dbReference type="STRING" id="418459.E3K7G1"/>
    <dbReference type="GeneID" id="10539291"/>
    <dbReference type="KEGG" id="pgr:PGTG_06241"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06241"/>
    <dbReference type="HOGENOM" id="CLU_514970_0_0_1"/>
    <dbReference type="InParanoid" id="E3K7G1"/>
    <dbReference type="OrthoDB" id="2510592at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173065" evidence="2">
        <location>
            <begin position="21"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="300"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="441"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="510"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="257"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="510"/>
            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP80285.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="529" mass="57778" checksum="3D1CFE7714C61E97" modified="2012-04-18" version="2">MLSIYLILLLWFQVVHQSSAAESSAAGASHEMNEGLTGEAMGSHKPSRGKRHFGNMLKLCRGETTAICPEQKLNSNPEREPVANSLVDGQLDDHRPAQRALNSRPPPPKSPPPPAPSHSRPPPPPPPPPPCPPPPCPPSPGSPPPCPPPPYPPSPPPEPVEHSYEGLGSFQEHAGSPPHPSTGVDPEEQEIEAAILESLRTSQPSPARQIHSEQSPLGNENILLPPMSRPPPRPPRSHSPPFHPPPPRRVPAREPRTSSQEPTENYHQQFHGIQEDAGPSSRPSTSVDIAELEIQAAILGSLKTKKPSLARRIRSQQSPSQTEAIPPRPVSRPPPGPPHSHSSPLRPRPPRRARAREPRASSQEPVGNHHQQFDGFQEYAGPSTHHPSPSSYEMHLAGMTTEEQDLRAAILESLKTNQRSHRVRKGPVSQGISWFKSLVGGRAERTKDDSISLPLSEHLGWPSDGPARVPTADYNSQQARPQTPVRHSPYRPTHDYNGNLDEYQALSHAMQVSTQEVSRPSSPYMARQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KA41</accession>
    <name>E3KA41_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07275</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP81023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325442.1">
        <property type="nucleotide sequence ID" value="XM_003325394.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KA41"/>
    <dbReference type="GeneID" id="10536262"/>
    <dbReference type="KEGG" id="pgr:PGTG_07275"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07275"/>
    <dbReference type="HOGENOM" id="CLU_095050_0_0_1"/>
    <dbReference type="InParanoid" id="E3KA41"/>
    <dbReference type="OrthoDB" id="2509368at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173156" evidence="1">
        <location>
            <begin position="27"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="257" mass="27773" checksum="32F928E2BC50096F" modified="2011-01-11" version="1">MFSSSHHLTFVTLVLTATWLARTTLAAVNCDTKFGSVDTNECAQAVSQIVYEKPGNTLDRVSTAFAKLYGNCTIFVFNPEKAVVTKQQIETGFTSIFDQCKAFTGQVSLSNSSFIQVSEHRRGYDIGYIEPQTLTCGLNTNAPLTVDKDCQAAYNSIPVDKQSRLLGDNGQPTTTIFKTFKTCTLQIFTTDESVLIGTKSEIGSVVSKTIKDCKGKSGVIASSKGGAGINGLTVVKVRSSLKCADRRDPPGKYQTCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAB7</accession>
    <name>E3KAB7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">DASH complex subunit DUO1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Outer kinetochore protein DUO1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PGTG_06895</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex DUO1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325693.1">
        <property type="nucleotide sequence ID" value="XM_003325645.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KAB7"/>
    <dbReference type="STRING" id="418459.E3KAB7"/>
    <dbReference type="GeneID" id="10536761"/>
    <dbReference type="KEGG" id="pgr:PGTG_06895"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06895"/>
    <dbReference type="eggNOG" id="ENOG502SCC0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1111603_0_0_1"/>
    <dbReference type="InParanoid" id="E3KAB7"/>
    <dbReference type="OMA" id="QWQGASQ"/>
    <dbReference type="OrthoDB" id="5599235at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013960">
        <property type="entry name" value="DASH_Duo1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28216">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DUO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28216:SF1">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DUO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08651">
        <property type="entry name" value="DASH_Duo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="7" id="KW-0132">Cell division</keyword>
    <keyword evidence="16" id="KW-0137">Centromere</keyword>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="10" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="11" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="8" id="KW-0493">Microtubule</keyword>
    <keyword evidence="9" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="29"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="196"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="46"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="67"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="107"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="196"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="246"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="EFP81274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="279" mass="31400" checksum="B65C30CC01B2023A" modified="2011-01-11" version="1">MPNKSNDPRGASSGKWSYDADILNDSISMNMEDDSFDGNESTFILPSNPAGPSSSSNPRSIPTGSSQPVPSSSQNRNNPAGDHHQFSSSPEKEPAEEPRRRRQPKASSKDQWVRRETERNRELENERDWLRDINQLLEKSADEMDQIKGKLENVQSATETSHELLDLYSKILTQTEHTKDLISNPNWEGLTADMKRRAEMKQAEKARLAQEREREREEELLRERKQQQLEAEAAAEAARKQSQLQRSTRGMGTSTRGRGAGTTAGRTAGGKSRIGRITR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAY1</accession>
    <name>E3KAY1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06836</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81215.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325634.2">
        <property type="nucleotide sequence ID" value="XM_003325586.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KAY1"/>
    <dbReference type="GeneID" id="10536700"/>
    <dbReference type="KEGG" id="pgr:PGTG_06836"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06836"/>
    <dbReference type="HOGENOM" id="CLU_026173_1_0_1"/>
    <dbReference type="InParanoid" id="E3KAY1"/>
    <dbReference type="OrthoDB" id="2509948at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="439"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="446"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81215.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="469" mass="50372" checksum="3FB6BD43483D8AF6" modified="2012-04-18" version="2">MNQTWPQQPFFQTENGYNTRIRYLEEVVHLLIARTNLVPPSPISSPPLSSSFRYSTERGLIPLLSKGTAESLEIASRSDRTYYFNPSPHTRSPTAHLISARLFKPRPRSCPIVTRGKSPLSVLSKDENATRDPKSARPAILTAPPPVLNKFGSSATGPDHWSSSNMRLTCSSRPPSCASVTGLSSQNPDVWTFLCTASKQLSSPDRAPSTSTDAAFASKIAVTAQDPTAISPLLDPSAQTAKLPTSGSDPATSPTSSHSKNNLSPRPSTPDTTNTSDHDPISVIVIKADNPLPTSFLTNHIACTAATATQMSLTPNQTMANTLPSTLSSSDCTPTKQCPKSENILSSIVTPPELAAAPLAPPESFESKSVLSSTTNCSSSLICPTAQNCLENLEVAAVGGIEILDNDDIDSIEAQIAPEYSQATLDYYNDIQEYLQQANADETETKKKRKKKKKANPTSTPNNPVLFYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KB15</accession>
    <name>E3KB15_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326160.1">
        <property type="nucleotide sequence ID" value="XM_003326112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KB15"/>
    <dbReference type="GeneID" id="10533449"/>
    <dbReference type="KEGG" id="pgr:PGTG_07990"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07990"/>
    <dbReference type="HOGENOM" id="CLU_015326_0_0_1"/>
    <dbReference type="InParanoid" id="E3KB15"/>
    <dbReference type="OrthoDB" id="2518090at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171873" evidence="1">
        <location>
            <begin position="26"/>
            <end position="661"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="661" mass="75278" checksum="D712E72ADB9C775A" modified="2011-01-11" version="1">MASSLQSWLLGSSFILTCLLKLSTASPPLSIYADSELPSVSAVGRRPEGSSIPFEALSIFDRQPDSKRQKTTIDFFGDLGDLGDGLHTGFGTPGNHVAQLDAPIESVGAAGSNLSREWRGSPVPHSSIDKSEHNTVSQVVRSSAGTPGDFEIRILNAQWAHILALTRKTIRKQDNPPDMTKIMQRLDKEIRLVQSALRHGIVEEKFELKGLPITVMIVRHRSTHRNPRRLLIKLDGLRPDAGSSHRRLEDTKNRMANILEALEYFHRLAREQGVDEKIFGPQIKNIDTDLNQWFSDILFEKTEDSYPLFGRFSDEFISKTSPEASFGSVQKAISKVLMYWKRPKVEKIYEVALSLLGYWYEINASKLGRDYLKNNPENYWSLMAQLVVEKAPRDGRISREYDQGEDFFTTNLKRYMIGKQTRQKWDWDALFELVDSRQVGSSMTKDIRDRIEAKTKSSGISRKPKVKEFETPIAGLPIYLIRALQPKHGPRMQKVRIILSQSKQWRRRRANQLSLLQRRIFLIYDSLDILHKIASEQEIDTKLLGSSVTDTQEKLMNWFLEVLFIHTEDSLPIFGTVRIPFPTAQPPAELFGAAQKYLSIMLTSPGKITRTHTNDIAFLLLGFWYEEVASKHGKEVLGLDAPHSYWKCMNQLSQKIKTGLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>E3KB21</accession>
    <name>E3KB21_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein OS-9 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_07996</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Lumenal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the OS-9 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81747.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326166.2">
        <property type="nucleotide sequence ID" value="XM_003326118.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KB21"/>
    <dbReference type="STRING" id="418459.E3KB21"/>
    <dbReference type="GeneID" id="10533455"/>
    <dbReference type="KEGG" id="pgr:PGTG_07996"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07996"/>
    <dbReference type="eggNOG" id="KOG3394">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_402318_0_0_1"/>
    <dbReference type="InParanoid" id="E3KB21"/>
    <dbReference type="OrthoDB" id="448954at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005788">
        <property type="term" value="C:endoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030970">
        <property type="term" value="P:retrograde protein transport, ER to cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.130.10">
        <property type="entry name" value="Mannose-6-phosphate receptor binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009011">
        <property type="entry name" value="Man6P_isomerase_rcpt-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044865">
        <property type="entry name" value="MRH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045149">
        <property type="entry name" value="OS-9-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012913">
        <property type="entry name" value="OS9-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15414:SF0">
        <property type="entry name" value="ENDOPLASMIC RETICULUM LECTIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15414">
        <property type="entry name" value="OS-9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07915">
        <property type="entry name" value="PRKCSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50911">
        <property type="entry name" value="Mannose 6-phosphate receptor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51914">
        <property type="entry name" value="MRH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0430">Lectin</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173180" description="Protein OS-9 homolog" evidence="9">
        <location>
            <begin position="25"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="domain" description="MRH" evidence="10">
        <location>
            <begin position="160"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="111"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="380"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="576"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="111"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="380"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="412"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="454"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="511"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="524"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="552"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="576"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="589"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="637"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="656"/>
            <end position="671"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="672"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP81747.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="684" mass="75393" checksum="C17214345FD71627" modified="2012-04-18" version="2">MNRRNVSPCQTLLLLLHLFQHASSLSSHSPPDPLAYPKYSVSLSGWDDAINNHTATAILSNLQSESKDDAVSILNYNSFSQNDQAELRHTLMRTASGQAFLCALPPIPPSASSKTAATPKSNLPDLPSSDTEDLERKKQREKIEEEGLKNGLKLISSLKDRCIYTKLGWFTYSFCYGKGEIKQFHAIMVPGFNHPQEDPNQDVYVLGFHLDHPQNPHHQARLEGTLPAQSISAGEGELTSLGRNRFASGGGSILGSISDVLRDESVIKSSVEKEEEEFQQKRYLVQRWDGGTTCDMTGKPRSVEVQFHCSTLGSDHIALLRETSICEYLLVIHTPRLCSEPLFRDGGARKGIGAGDMVANIGCRPVLTNEEIEKWKANEEARKKKDEEEKLKKSQSLDETPKENTKADESFTDSVKSTTSSEAPAESDASNVPSHQEKLNESPDNEEQAPDSIPDQTVLSQNTDQATDGADSQQSANKEDSQKTTVKGETLEELEIDPITVYFDADTGKMYMDKSAADEARGRPSSTTADGKTQGTKSDSKTEGKNSPSDQANKDSNELEEIAKALKESLGALLRDLRGDDEGNAGRRLNTNRVNQRPRSLAEVLASFKSGDSPLGTKAKLKNQKFTDGHPPAQTPEDIKKLSSESHSKLVEMYQQKFNIQSDNQPVPNNKETQEDSNKEEDKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBL3</accession>
    <name>E3KBL3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NUDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_07944</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nudE family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326114.1">
        <property type="nucleotide sequence ID" value="XM_003326066.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KBL3"/>
    <dbReference type="STRING" id="418459.E3KBL3"/>
    <dbReference type="GeneID" id="10533402"/>
    <dbReference type="KEGG" id="pgr:PGTG_07944"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07944"/>
    <dbReference type="eggNOG" id="KOG1853">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_491005_0_0_1"/>
    <dbReference type="InParanoid" id="E3KBL3"/>
    <dbReference type="OMA" id="TITTNIH"/>
    <dbReference type="OrthoDB" id="5877028at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051642">
        <property type="term" value="P:centrosome localization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000132">
        <property type="term" value="P:establishment of mitotic spindle orientation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047496">
        <property type="term" value="P:vesicle transport along microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033494">
        <property type="entry name" value="NUDE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006964">
        <property type="entry name" value="NUDE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10921:SF1">
        <property type="entry name" value="NUCLEAR DISTRIBUTION PROTEIN NUDE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10921">
        <property type="entry name" value="NUCLEAR DISTRIBUTION PROTEIN NUDE HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04880">
        <property type="entry name" value="NUDE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NUDE" evidence="9">
        <location>
            <begin position="132"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="187"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="306"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="386"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="27"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="209"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="386"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="417"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="440"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="487"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="532"/>
            <end position="545"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP81695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="554" mass="62101" checksum="5FC3CE89961056E4" modified="2011-01-11" version="1">MEEPSFTSDGDALDYYRSLARSLQSDLDDTKLALDEFQVSSKELETELERELQATEKQLKELRGKEEHLLHEIDQWKIKYHSSLKDHTKTMTHMQTELETCRKSNEEYRTRLRDMELDNDELEGKERMVASSLQDVESKYGKAIERITLLEDELIEKSKLEEEHQRLKDDLRDMTEELAVLREHLASSATSPAGEATRRVLSPSPEDCQLSTRTFSETPLSDPGLEVLKDTDEMSSPSRPMIRTKPTSVIDAQDSHRRRLSSMAGEIGSPKSQTAKSLAITQSKKAASLRVKPHSVTIKPLQNVFSPDPRLAASTSVAPRAKRSQSRSRPALETPTNPRNLNKESSHLQSLRAETERMQGMTKKLVSSRNLRTVSAIPVPKTSLLSSSIHSKSNPHSISALNRSSTTRSPRRPPNGVSLTASRNVTQPATSQDRDLDTGLPRPRSTRPLSRLSISSDQQQQQQHQLGISMMGPSDAPPSCAIPRRKASIDMTERFPRPLSIQDVHKSHLRRQSTGFTGPPPKLDPNDRKPKFSSNPLGKSFSTSIRAAGLSFKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3KC30</accession>
    <name>E3KC30_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">HIT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_08061</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Required for box C/D snoRNAs accumulation involved in snoRNA processing, snoRNA transport to the nucleolus and ribosome biogenesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the BCD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EFP81812.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326231.2">
        <property type="nucleotide sequence ID" value="XM_003326183.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KC30"/>
    <dbReference type="STRING" id="418459.E3KC30"/>
    <dbReference type="GeneID" id="10533683"/>
    <dbReference type="KEGG" id="pgr:PGTG_08061"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08061"/>
    <dbReference type="HOGENOM" id="CLU_1086386_0_0_1"/>
    <dbReference type="InParanoid" id="E3KC30"/>
    <dbReference type="OrthoDB" id="272357at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070761">
        <property type="term" value="C:pre-snoRNP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000492">
        <property type="term" value="P:box C/D snoRNP assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23023">
        <property type="entry name" value="zf-HIT_BCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051639">
        <property type="entry name" value="BCD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057721">
        <property type="entry name" value="BCD1_alpha/beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007529">
        <property type="entry name" value="Znf_HIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13483:SF3">
        <property type="entry name" value="BOX C_D SNORNA PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13483">
        <property type="entry name" value="BOX C_D SNORNA PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25790">
        <property type="entry name" value="BCD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04438">
        <property type="entry name" value="zf-HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51083">
        <property type="entry name" value="ZF_HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HIT-type" evidence="9">
        <location>
            <begin position="24"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="174"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="184"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="210"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="228"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="245"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP81812.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="256" mass="28882" checksum="1857D781B60C2EBA" modified="2012-04-18" version="2">MEASPSTSPPTPAQERPPARSKTCEHCQTTGSKYTCPGCGTRTCSMDCSNGHKASKNCSGKRNRVEYVPLNQYSWGRLMQDYSYLEEIHRHLSHHPPPSSVSNIPKPVGPPLGLTPPSRNSIAKRQLLVRMAALEAVRLLLMPDGMSRRTMNMTNYNHKKKCMQWSVELVFPSDEQTQEAVPQPTSQDRSSSSSTLTPPPPPTTPTVTSNQHTSHLIHQISSDTTIIELREKDKQDTRDSDEKPRWRRRRLIKEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>E3KC97</accession>
    <name>E3KC97_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="10">STE protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_08104</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EFP81855.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326274.2">
        <property type="nucleotide sequence ID" value="XM_003326226.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KC97"/>
    <dbReference type="STRING" id="418459.E3KC97"/>
    <dbReference type="GeneID" id="10533892"/>
    <dbReference type="KEGG" id="pgr:PGTG_08104"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08104"/>
    <dbReference type="eggNOG" id="KOG0198">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003051_3_1_1"/>
    <dbReference type="InParanoid" id="E3KC97"/>
    <dbReference type="OrthoDB" id="266718at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004709">
        <property type="term" value="F:MAP kinase kinase kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000165">
        <property type="term" value="P:MAPK cascade"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000334">
        <property type="entry name" value="Serine/threonine-protein kinase STE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000387">
        <property type="entry name" value="Serine/threonine-protein kinase STE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029458">
        <property type="entry name" value="Ras-bd_By2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584:SF369">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 19-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11584">
        <property type="entry name" value="SERINE/THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14847">
        <property type="entry name" value="Ras_bdg_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01304">
        <property type="entry name" value="Ras_bdg_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="9">
        <location>
            <begin position="773"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="308"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="373"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="455"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="615"/>
            <end position="768"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1040"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="37"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="49"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="308"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="403"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="484"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="502"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="521"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="562"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="615"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="638"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="694"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="705"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1052"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1096"/>
            <end position="1118"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="802"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP81855.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1140" mass="122306" checksum="5CF5C4D2D664AD3F" modified="2012-04-18" version="2">MATNSSSSSSGAATPPAVPVSQTSLFLKTSDHRRAPSLNSTSIGSNSKPISIVPSPGNNNPSSSNTVASSSPTSTGSPSRGLNTGTLPGSNPVGLTCILEDGTAGNLGGENGSGRGGGGMGEIYGAAGPREIGFKVGRGGFGRPTTAAGTVGVVAGTTSQIGPVCQPTLDEVIRKTIKVTLAENGIMKTINVENLKSAVDLLDRVLRKFGNINASGQASAGRSVNLDDWALSPMPLNAVPGELNWGTIKQLDENELLELCRTTNRPERQRGLLLHYVATGKRANRKAASPYFGAEEIVVSGVSPTMSPTIAGSSPTLSAHQQSKQEIREGGEPLTGADEGGSMEGKTKPDIPAPRKTRNRASVVSVMSGLMIDPSRHSEPLPPDYQASLQQHGSHNHHPEDGVSGGTAAGGGGERVTSLLASGRGKLRNFFGHRPPSEIISNHLVDYFPSVENKKHLSKTVRQSVRKSMAIRRSSQFSNNNNLPGGGGGGGNGGRTTSWDMSSYPSLPLQPQQQNHPGRLSTDSNRSTHITASKSFEERSSPHISIADFSETSEPMDRSRASLDTLNSLPDPAISNNRLSTRKQRDSDLTSILTLDEVTAEVENRRLSIMALGVPGLLPSKSSSSSVSDWGSLSGLLTSKNPSDPVLSSTDDPSNELDDRHSHPAGGHKPAPTRRSLSSTRIQDPILEENEASDTLKPDGRVDQDQDNQDEEDEEDDEDDEEEDDEEDEEDDDDDDDDDDDEDDEDDDDEENKDANDDPQKMTSSGSKASIKWVRGALIGQGSFGSVYLGMHALNGTLMAVKQVERPSGTSHNEERKKSMLGALVREIEFLKELQHTNIVQYLDSSADNAFFNIFLEYVPGGSVSTLLKNYGSFEEALVNSFTRQILDGLIYLHSKEIIHRDIKGANILVDNKGVIKISDFGISKRVEDNLLSTARIHRPSLQGSVFWMAPEVVKQTSYTRKADIWSLGCLIVEMLTGEHPWASLTQMQAIFRIGSFATPEIPDDISEECIDLLKQTFLIDHHARPTAMELSNHAFFRSEEGGGGVVGKSETSVEKGGANREKEGEGEKKEQVQEAHEADESRGKSEEEGGGGAVDGEKELEVSEDGKTDTDSIIENEARCLVDEANLELSRRKSALANQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCD9</accession>
    <name>E3KCD9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08136</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EFP81887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326306.1">
        <property type="nucleotide sequence ID" value="XM_003326258.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCD9"/>
    <dbReference type="GeneID" id="10533924"/>
    <dbReference type="KEGG" id="pgr:PGTG_08136"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08136"/>
    <dbReference type="HOGENOM" id="CLU_883209_0_0_1"/>
    <dbReference type="InParanoid" id="E3KCD9"/>
    <dbReference type="OrthoDB" id="2513131at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="315" mass="34808" checksum="49C2FBFBDD24B687" modified="2011-01-11" version="1">MAPAGAAQPMILLDPSPALMSPPAGPVNSIRPGCTLTRHTLAGGPPSHGCGDAMRPQNQHASRPTSHAPYRRLTSARPKCSLQSAWHQHNRCPLRPAYLTTGLLSFSAVGILQNLKSCKTASNNPFSRQRNIPRLAQFILAFNRWSSSTGGYPSAQPASFQRQGVNKGKKRESVLEESSNHSPKRALNNHQSNSPMASTSQGRVNTVEMERMTLGELFVERQLAEAQFDECPQNQSFKHNIKPSGRHYPQGPRQPPSGLFSQPATPWMSQDQAPRTEDILDSHFISLFHNHLMQSDIINPLSAFHTWPLHVFHSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KD70</accession>
    <name>E3KD70_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82103.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326522.2">
        <property type="nucleotide sequence ID" value="XM_003326474.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KD70"/>
    <dbReference type="GeneID" id="10534634"/>
    <dbReference type="KEGG" id="pgr:PGTG_07500"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07500"/>
    <dbReference type="HOGENOM" id="CLU_977085_0_0_1"/>
    <dbReference type="InParanoid" id="E3KD70"/>
    <dbReference type="OrthoDB" id="2496311at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171911" evidence="1">
        <location>
            <begin position="22"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82103.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="285" mass="32274" checksum="2E8E764CF758D457" modified="2012-04-18" version="2">MRFTFLTLTASLLLLNHQAFAGEDNIDPPLNPTSTIQSFSFQRKLSWTNQDFDVTQDGNNAHIMSVEAHLKDKWMKAHFALDLVLPSGERVGIKVEGKLVHCGFTQTYKISNGVQLLVDPRGTSTDRWYIKKSGYIKQAYTWHRHFQGLSGVVTGEHGRKVAYFSSLHSRFPLGYYSSLLSWQVSIHATFHMYSTHIFTIIINSSIVDKNTKNYRETLIDFLNFERQTLKSGLTLCMPPSGVFGRRVFTLLGLEADRKILIRVISEGQTECSQSIPAGVVRLKSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3KD81</accession>
    <name>E3KD81_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_07704</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="KEGG" id="pgr:PGTG_07704"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07704"/>
    <dbReference type="HOGENOM" id="CLU_429688_0_0_1"/>
    <dbReference type="InParanoid" id="E3KD81"/>
    <dbReference type="OMA" id="QQPETHC"/>
    <dbReference type="OrthoDB" id="2596255at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
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        <location>
            <begin position="25"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="176"/>
            <end position="305"/>
        </location>
    </feature>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <begin position="562"/>
            <end position="621"/>
        </location>
    </feature>
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        <location>
            <begin position="359"/>
            <end position="447"/>
        </location>
    </feature>
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            <begin position="25"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
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            <begin position="176"/>
            <end position="186"/>
        </location>
    </feature>
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            <begin position="245"/>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="589"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP82307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="638" mass="70888" checksum="70C3738EEC37D4CC" modified="2011-01-11" version="1">MQHLLRSIYNNQWFPFQTIPLLSSPKSTPASSPAVDLSPSLSSSSSTSSSSSGNSTLRPSLTPTEEGDHHFQLDSSDQFHLLQITSTSLSISVSLPSSYLNNQLIHPLINLNSQPWSKLLLLDQSQPSNRASHPDQDGTSLASLTILIYGLEPGTVYDVDLELNLVDQHPALNLASNSSLRNQPTDDIQHNLPQKLDSTSTTRLELNDQPPARQHSHRLKLSDARARSQTSIPHMDPDGPPPPYTSYDPASPTQTCTSDVVRSSSHSFSPLESSFAEITSTETSLDASTLPAPRSDNTNEEDQAMRTEVEKQALALREAIEENAPLKQSLCDLEQLIKRLDEENVTKSAALLSYDEPKMVEFEKRIEDQKEKLKRKTEEYNALEQKNQRKLDTLKQNNQLLKTRWEESIQRVGDLKEKTLKPTEEEIEELTRQLSECEKEIKSIDRETQKRAIEHSNQFQIVRSRSASKKPNLIPSTSNSRGTSNDSAHKEASKNRRQSKWRSMDDFKSMNPSLVRRNHSGKTVALDEKTPAGSTTVVAQQQPKILYQHNRSRLNKSISIAACPSPNGSPEKKSRYMRGQLASTHSSLPATNTTAPTSTVTTPTPTTTTTTTTPVNHSHSVPVVSVSAKTWAEKLAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDP1</accession>
    <name>E3KDP1_PUCGT</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08433</name>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326896.1">
        <property type="nucleotide sequence ID" value="XM_003326848.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDP1"/>
    <dbReference type="GeneID" id="10529856"/>
    <dbReference type="KEGG" id="pgr:PGTG_08433"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08433"/>
    <dbReference type="HOGENOM" id="CLU_108601_0_1_1"/>
    <dbReference type="InParanoid" id="E3KDP1"/>
    <dbReference type="OrthoDB" id="2508299at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
    <dbReference type="InterPro" id="IPR007612">
        <property type="entry name" value="LOR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04525">
        <property type="entry name" value="LOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171923" evidence="1">
        <location>
            <begin position="23"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="220" mass="24502" checksum="CF9A29A0287EA2F4" modified="2011-01-11" version="1">MQFLPALISLLACLSFATRIAASSHGADPSPLSKVFIMKKEMDYSQGSLHVYAADGTIVYRFLRTIQDPITGFTTVFLESSPSKAFFVYLQSPNSLCGHKTNYTQLDAPGARKMQFTIEPNNFRKDKWSFSYVDGTGTQQNFKFDRNYASQEGEIRHQINGQDGNLVAELRNQKRTDSWLTSSSVGEVDTYTLSCTADSPQVELILLMGLVISRVHDCGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3KE18</accession>
    <name>E3KE18_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small-subunit processome Utp12 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_08331</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UTP5 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="STRING" id="418459.E3KE18"/>
    <dbReference type="GeneID" id="10529752"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08331"/>
    <dbReference type="eggNOG" id="KOG4547">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_717803_0_0_1"/>
    <dbReference type="InParanoid" id="E3KE18"/>
    <dbReference type="OMA" id="QWSLAAH"/>
    <dbReference type="OrthoDB" id="30195at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007148">
        <property type="entry name" value="SSU_processome_Utp12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052414">
        <property type="entry name" value="U3_snoRNA-assoc_WDR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44267">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44267:SF1">
        <property type="entry name" value="WD REPEAT-CONTAINING PROTEIN 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04003">
        <property type="entry name" value="Utp12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small-subunit processome Utp12" evidence="5">
        <location>
            <begin position="162"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="298"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="10"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="23"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="38"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="339"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="379"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="409"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP82375.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="459" mass="50101" checksum="6212508AA6BFE656" modified="2011-01-11" version="1">MPAKAATPKSKGRAPKASHKKLKQEAKNQQEPEKATVSRSPSPSSASSSSDEAPDKNAKSDQNGDEELNVQDETTTQSFADRLKILNLQTDTSNGPIDLNESDDDELPEGLEKEGLANKMLGSKSAKSLNGSLNDTNSINPTTIASIGSFAQTLTQALKSTDTKLLESCFLQQSKNNKNLISNTIQRVPKKLLHTLIEQIVICLNRKKRGYGDGVTVATVRRTKTLVEWVRQILLIHMSYLLTIPTLVTQLSTLQASLQKRLNLHTKLLSLNGRLELVLNQIELNRVSNGSRQLTGSKTLKAIDKKKKKATSVPKQEPYKYVEGESSASEQEAEKSNDDESDGSSDQSDDEDGSDSDQLDQTDSEEEGSVEDVVLGSGSDEEDSEDSEDEAAEGARRLKKLTNGINDLLDLEADESGSEDGSGDAEEEEEEEEEEDDDESMEGFIDDGSEESSEEEDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KF10</accession>
    <name>E3KF10_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10010</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178284">
        <property type="protein sequence ID" value="EFP83042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327461.1">
        <property type="nucleotide sequence ID" value="XM_003327413.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KF10"/>
    <dbReference type="GeneID" id="10544754"/>
    <dbReference type="KEGG" id="pgr:PGTG_10010"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10010"/>
    <dbReference type="HOGENOM" id="CLU_068103_0_0_1"/>
    <dbReference type="InParanoid" id="E3KF10"/>
    <dbReference type="OrthoDB" id="10323506at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="227"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="273" mass="30756" checksum="AED83836B1BC3E72" modified="2011-01-11" version="1">MSVVTPRVQPKDGWANGNGWALLAGPLSNRSRNPIVGSESPKRWKGCKPPHDIFRELTPGTLPQNQGSKKWDYKSNQVYVISICDFLWTQQLKIPELLNNKWLLRQTPHLELDLALPQDPQDKSNDSSVCAEKPVAQFCFISLPNFCILTGDYVTLPLEKLLWIFANSGSKQGKQSLPGWAEKDPTARSFVEKLCYNCLSPAEKLAYDRAVDMEKQLEGDKAIEQERVAEQSELLAAEQDIAKQERDLALKQIRHLKAVLKSLGADHPQQPDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3KFB0</accession>
    <name>E3KFB0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">SAC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_09905</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178284">
        <property type="protein sequence ID" value="EFP82937.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327356.2">
        <property type="nucleotide sequence ID" value="XM_003327308.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KFB0"/>
    <dbReference type="FunCoup" id="E3KFB0">
        <property type="interactions" value="1053"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KFB0"/>
    <dbReference type="GeneID" id="10544648"/>
    <dbReference type="KEGG" id="pgr:PGTG_09905"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09905"/>
    <dbReference type="eggNOG" id="KOG1889">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003016_7_4_1"/>
    <dbReference type="InParanoid" id="E3KFB0"/>
    <dbReference type="OrthoDB" id="405996at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043812">
        <property type="term" value="F:phosphatidylinositol-4-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662">
        <property type="entry name" value="PHOSPHATIDYLINOSITIDE PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45662:SF2">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3-PHOSPHATASE SAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="587"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="615"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="domain" description="SAC" evidence="3">
        <location>
            <begin position="153"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="692"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="703"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="723"/>
            <end position="733"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP82937.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="733" mass="83257" checksum="7A11A0ACD1D2C633" modified="2012-04-18" version="2">MTNNNNLGIHSSLNLYISDQAYVLTSATDNQSQSLTINRKDSQISLSSPGPPEKFDRLVNNVAGLLGIIRLMKSDYLVLIQSARKVTNVFKTAVYTPSKFAVYPISLEPNLSILENSDERYLLSVLKAHLDHALDKTFFTYLSKTHPNDPEPWNLTNSLQRQGSITKQNKQADGAVSDEQQEQPPWKTSDDRFFWNKFIQTRFIELASQPNGNQASKFILPVIFGFLEFKSAVIKGKRFTFGIVSRRSRYRAGTRYFTRGINSEGDVSNFNETEMIMTTFPPNYNTQANGPTDPGNGRSFVKAGFVQTRGSVPLFWTEINNLRYRPDLKIIDLPESLEAMKAHFDQQVSIYGDQYLFNLVNSSGYEKAVKDGYERAVKELNNPRVHYTYFDFHQECKGLRFDRVQILIDQLHDQLVDQAYFFQEFSAGPSSNPASKVQKSVVRTNCMDCLDRTNVLQSALAKWVLTFQLKQAGILEENESLDKYPDFMFLFRNLWADNADGVSKSYSGTPALKTDFTRLGVRTKKGAFDDGVNSLMRYVKNNFMDGPRQDSYDLFTGAWRPPVSDDPQATQPLHDQHLFKGPYDHHLIQAIVLGFVIFLLIFISATFTQKRSLKLAIISLIILSLITRYIFQHGMEFVNQPRLKGSTTNATRHRILSYNGPSSRIFQSSQHGRTKINFDLFDHFVSLLSTNNHHHLNPPPPHSSSTAAPPLSSSISGPNQSHVLDEDKKKRLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3KFZ7</accession>
    <name>E3KFZ7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Wax synthase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_09221</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Secondary metabolite biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the wax synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EFP83268.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327687.2">
        <property type="nucleotide sequence ID" value="XM_003327639.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KFZ7"/>
    <dbReference type="GeneID" id="10544982"/>
    <dbReference type="KEGG" id="pgr:PGTG_09221"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09221"/>
    <dbReference type="eggNOG" id="ENOG502SAAK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_633232_0_0_1"/>
    <dbReference type="InParanoid" id="E3KFZ7"/>
    <dbReference type="OrthoDB" id="2499988at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044851">
        <property type="entry name" value="Wax_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032805">
        <property type="entry name" value="Wax_synthase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31595">
        <property type="entry name" value="LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31595:SF57">
        <property type="entry name" value="OS04G0481900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13813">
        <property type="entry name" value="MBOAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Wax synthase" evidence="9">
        <location>
            <begin position="324"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="110"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="141"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP83268.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="496" mass="55423" checksum="E97D36E732FF18E2" modified="2012-04-18" version="2">MHFFEYLTHPKLLGHAGLMIPLTIQAALLHPYYQQNVTSSVVRRSLIPIAVIMAFVTAPMRKWLPLEDSMMVNFCFVSLVTFHATCLAFEYGLHEGPVLGPKCELEEVELSGEENHTSISPEDLIKSQSIGTTEARKRTNNHASTDGETKSTTMTGDSSESNVKNSPTKKKEQLVKPSTGELVRFVLWLLFSPRGLECQWAPHPSIVTFRKPISTSTFILRETLGRVFVLHAYGTVCWALATCAFHHPQGFYGIIKDVTGLSESSVIKFFAGYGTSFCIGACMWTGLEIAAAGMNLFEFLLYSIGRRFLPVSWAPEKPFNPRRYPPLFSKPWESKSMSEFWGRGWHALFRRDLTFCGALPAFKLAAMLGFGKQVQKLSGLMGAMFLSGLMHEYAIASISWEKTGPQIIFFFMECAIVMAVENLIEKHTKIRFKGIWGTIWTFGFLMYFGKPGLDLCGAGVKFFSLLDLHTLVGDAFGGTSEALQYQAGPVEGQYQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>E3KG09</accession>
    <name>E3KG09_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Endoplasmic oxidoreductin-1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PGTG_09233</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="4">May function both as a monomer and a homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Lumenal side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the EROs family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EFP83280.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327699.2">
        <property type="nucleotide sequence ID" value="XM_003327651.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KG09"/>
    <dbReference type="FunCoup" id="E3KG09">
        <property type="interactions" value="375"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KG09"/>
    <dbReference type="GeneID" id="10544995"/>
    <dbReference type="KEGG" id="pgr:PGTG_09233"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09233"/>
    <dbReference type="eggNOG" id="KOG2608">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023061_1_1_1"/>
    <dbReference type="InParanoid" id="E3KG09"/>
    <dbReference type="OrthoDB" id="269384at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016972">
        <property type="term" value="F:thiol oxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034975">
        <property type="term" value="P:protein folding in endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007266">
        <property type="entry name" value="Ero1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037192">
        <property type="entry name" value="ERO1-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12613:SF0">
        <property type="entry name" value="ERO1-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12613">
        <property type="entry name" value="ERO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04137">
        <property type="entry name" value="ERO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110019">
        <property type="entry name" value="ERO1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9" id="KW-0274">FAD</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="14" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="52"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="548"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="693"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="13"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="568"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="595"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="612"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="16">
        <location>
            <begin position="695"/>
            <end position="713"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EFP83280.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="19">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="713" mass="80419" checksum="68149591D5208B5B" modified="2012-04-18" version="2">MTPLDAKPPSRLASPSTSTQPDPRPDNSTTNQTSQPSQIIYFSFYISEHLDISTQDTPQSTHKEDRYPPRTSIYPEMHHPHRTKRNSILTTTLILFHSSALVSSFESPPTIPTQQLTCKPTGLIQDTSCNFETIDEVNRKLHSEISDAVQLPIFRYHKVDLYRECPFWSEDGLCGNRACAVDELDQNDIPAYWRNSELSDLKTSSLKDIPIDMKDKCVVKEQDYCVLDGDNGMPSNGVYVDLLANPERFTGYSGKSAQRIWRAIYEDNCFDVPQRPAFSPFSTPPPSSRAGLDPHPDLVEPGNADNGTCLEKRVYYKLLSGLHTSISMHICDEYLDPSTGQWVPNLQCYLNRIGLYPERLENLYFTYTLMLRALSRSASYLSSPRVELCTGDVQSDSLTRKSLDRLIAIAHSYPVTFDETSMFKSNPHGHNTLKDEFKEHFRNVSRIMDCVGCDKCRLWGKLQVMGLATGLKLLFEYEEGSQDHSNFHLTRPELLGFINTLNRLSESLAAVDKFTKLWEQRFAEGTLPNQIRDEKFDGLASGKAAQAISLSPGLSPRPQSPFAGMPATEKEKPKKPKTKLDSKPNDQSSKPTASPPLSSTNSQTPPSPSNSKAKLTYSSNTHSPSNNRSDPTIQSNIRSHVIDLGLLVRPASKMFKMFSDKCKESFAMCSHWVHSLLGQMKTLVFLSRSHNQNHHFQDQDLHQRSSRKEKNEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHA7</accession>
    <name>E3KHA7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09395</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83682.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328101.2">
        <property type="nucleotide sequence ID" value="XM_003328053.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHA7"/>
    <dbReference type="GeneID" id="10532368"/>
    <dbReference type="KEGG" id="pgr:PGTG_09395"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09395"/>
    <dbReference type="HOGENOM" id="CLU_085808_0_0_1"/>
    <dbReference type="InParanoid" id="E3KHA7"/>
    <dbReference type="OrthoDB" id="2499434at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="146"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83682.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="274" mass="29886" checksum="3C3724A028CA4F21" modified="2012-04-18" version="2">MGSESPASSKDLPYESSVPSDQQDMLEYSSLATGSPTSLPGCASLAGEVNPVLEDPWMAMKRRAILSVLNRPASYARGLPDFMPGSLSRSVQDMKMKGKQAVRDCSPLPPSQIEHPVSPKTKAPADRIVHLVSRVGGTYQSPYPLSSEKGSSIPPSSGSSESTVGPMSPRHDAPPAFKRSRIESDRPFSPLNTTDYLDLSPSQIDELLHEGKEFPTRFIYTAEQLNEIAHCCLIDLIDFFNFYAPGSPQEASARVHLVTRHQGHFNAFLKATKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHZ4</accession>
    <name>E3KHZ4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">2-methylcitrate dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09632</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EFP83919.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328338.2">
        <property type="nucleotide sequence ID" value="XM_003328290.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHZ4"/>
    <dbReference type="STRING" id="418459.E3KHZ4"/>
    <dbReference type="GeneID" id="10532609"/>
    <dbReference type="KEGG" id="pgr:PGTG_09632"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09632"/>
    <dbReference type="HOGENOM" id="CLU_2098043_0_0_1"/>
    <dbReference type="InParanoid" id="E3KHZ4"/>
    <dbReference type="OrthoDB" id="5404100at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.120:FF:000006">
        <property type="entry name" value="2-methylcitrate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.120">
        <property type="entry name" value="2-methylcitrate dehydratase PrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036148">
        <property type="entry name" value="MmgE/PrpD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042188">
        <property type="entry name" value="MmgE/PrpD_sf_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005656">
        <property type="entry name" value="MmgE_PrpD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045337">
        <property type="entry name" value="MmgE_PrpD_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16943">
        <property type="entry name" value="2-METHYLCITRATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16943:SF16">
        <property type="entry name" value="2-METHYLCITRATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19305">
        <property type="entry name" value="MmgE_PrpD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103378">
        <property type="entry name" value="2-methylcitrate dehydratase PrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MmgE/PrpD C-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF19305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83919.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="116" mass="13267" checksum="0668B6799336C3A8" modified="2012-04-18" version="2">MVTIPLIFGRLTTGDYTDKVALDLQIDELRAKIICTEEKKYSAEYHPPNKRSIGNAIMIELKDGTVLDKAEIKYSVGHKPRREEGKPLLDAKLKRHIKTHNRLPLSSFQNTPTSFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIC4</accession>
    <name>E3KIC4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DUF803-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_09762</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015693">
        <property type="term" value="P:magnesium ion transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="EmrE-like"/>
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        <property type="entry name" value="Mg_trans_NIPA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570:SF85">
        <property type="entry name" value="DUF803 DOMAIN MEMBRANE PROTEIN (AFU_ORTHOLOGUE AFUA_1G15880)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05653">
        <property type="entry name" value="Mg_trans_NIPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="174"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="242"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="272"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="340"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="388"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="404"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="437"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP84049.2"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="468" mass="51164" checksum="ACFF58DEA3501B71" modified="2012-04-18" version="2">MGVEDKYIGLALAVSSSLAIGTSFIITKKGLMDAAERVSGPSTDTHTYLKNPIWWAGMTTMVVGEVANFAAYTFAPPILVTPLGALSVLIGAVLASFFLQEKLGRIGKIGCALCLLGSIIIVLHAPEDKEVKTVDEILGYAMHPGFLFYCFFVLVFSVFMIYKVSPTYGTREPIVYISICSLVGSVSVMAIKGFGVAIKLTLAGSNQLTHLPTYLFAIVVAGCIVVQMNYFNKALDQFSTNVVNPIYYVCFSTATIVSSLILFQGFGTQDAVNTLSLLMGFFVTFLGVYLLNISRLDPTGTAQNQDRSLENGVMHPRMSLNASRLSLSSEQPLTHHPIHRSYHRSSMPSSANPFHPSPKGQLGAYRSQDSVLFDAFPEESVGLTLMNTSEEEEDDDSHHPDHRDHHHHRLSSPHRNHSTSPRKSKFPIVSLREVKLSPTTTTPTDNRLSPLNTDTNPLPSTDHSSPHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3KKJ0</accession>
    <name>E3KKJ0_PUCGT</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10286</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178292">
        <property type="protein sequence ID" value="EFP84815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003329186.1"/>
    </dbReference>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10286"/>
    <dbReference type="HOGENOM" id="CLU_739953_0_0_1"/>
    <dbReference type="InParanoid" id="E3KKJ0"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172048" evidence="1">
        <location>
            <begin position="20"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="374" mass="36426" checksum="5CF451C44371E7DB" modified="2011-01-11" version="1">MLSSATLSLALLFASATNALPNGYSSSSVSRSYTGTYPIIAQPVQPVQPVQRSFYGGQQPYFGQQQFYYGQQPSYYGYGSYLPNQAFYGYQPYPAWGQAYGAGPVPFAGQSYGYRSRTIYGGGPITGYPDQAFGNFEESQFGPATLARAGLQNSVQGGGEIGAGAQAISRAHVGAGGQVAGGGQFSGAGQIGGAALLNGNGQVVGGANAIGGGQLGGQAGGAGQFDAIARAGGNGQFGLGGQATGRGQVSGAGQFDAGARAIGNGQVGLDGQAIGRAQVGGAGHFDAGAHAIGNGQAVGQGQVGGAGQFNAGAHAVGNGQLGLGGQAVGRGQVGGSGQFDASARVNGNAQAVANGQLVGGGQVAGGSKIGAGLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLJ2</accession>
    <name>E3KLJ2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_11336</name>
    </gene>
    <organism evidence="1 2">
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        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP85167.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329586.2">
        <property type="nucleotide sequence ID" value="XM_003329538.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLJ2"/>
    <dbReference type="GeneID" id="10527148"/>
    <dbReference type="KEGG" id="pgr:PGTG_11336"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11336"/>
    <dbReference type="HOGENOM" id="CLU_131660_0_0_1"/>
    <dbReference type="InParanoid" id="E3KLJ2"/>
    <dbReference type="OrthoDB" id="10292259at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP85167.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="174" mass="19035" checksum="4DC3135581491E77" modified="2012-04-18" version="2">MAETLTNPCPSKEGSLGWKVESKKHGKLVKQQVLVPLSIDPSPKHPPLTVSVSQECLDSYSTSKTSTQLLFQTIKLDLKKAKRLVVRLPFGKVFHHLPGSLFSSFLNHRGQASPMRVFNPIKDQSLMGVCPPLMTVSLMGGLFTPVNDRSSMGVFTPIKDQSKPILDGGVSSHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3KMK7</accession>
    <name>E3KMK7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGTG_11888</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">May be involved in transcriptional regulation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the krueppel C2H2-type zinc-finger protein family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178295">
        <property type="protein sequence ID" value="EFP85532.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329951.2">
        <property type="nucleotide sequence ID" value="XM_003329903.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KMK7"/>
    <dbReference type="STRING" id="418459.E3KMK7"/>
    <dbReference type="GeneID" id="10544092"/>
    <dbReference type="KEGG" id="pgr:PGTG_11888"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11888"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_787874_0_0_1"/>
    <dbReference type="InParanoid" id="E3KMK7"/>
    <dbReference type="OrthoDB" id="2496855at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000292">
        <property type="entry name" value="zinc finger protein 619"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235">
        <property type="entry name" value="KRUEPPEL-LIKE TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23235:SF120">
        <property type="entry name" value="KRUPPEL-LIKE FACTOR 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="10" id="KW-0804">Transcription</keyword>
    <keyword evidence="8" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="292"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="55"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="127"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EFP85532.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="352" mass="38733" checksum="16F209D266396919" modified="2012-04-18" version="2">MNTFRNPSPSPARYGSSHARQSSMSDYGMRGGNVTSAPSGLSYSISKNDLAAYSLSTQQRESSLASVRGNPSSELRPSALATQVNCRLEKSGPNLSHSEALGSARPILPPIKSGKVISPITRAPVHSYPEHPSVPQNRGYHNAYPESLYESGRSSSKGIQRVLAHETSSLASNKNPPSYSQNYFPEAPRSKTLVKGTHGSISNLNPSSPLHPNLMPSPDLRYKAEPQMTHRRSDSSCELNFTSHYTGSRRPTDQLLHSGHPGLESVNMCHPNQDPIIDCNSNQLSPTTEKKHRCGFCSQAFARRHDRDRHQRMHTGEKPYECSQCGKRFMRSDALSRHRLVEPKCGGYLGQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KMZ7</accession>
    <name>E3KMZ7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10962</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EFP85633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330052.1">
        <property type="nucleotide sequence ID" value="XM_003330004.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KMZ7"/>
    <dbReference type="GeneID" id="10544198"/>
    <dbReference type="KEGG" id="pgr:PGTG_10962"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10962"/>
    <dbReference type="HOGENOM" id="CLU_2110121_0_0_1"/>
    <dbReference type="InParanoid" id="E3KMZ7"/>
    <dbReference type="OrthoDB" id="2495164at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="113" mass="12509" checksum="1041CCF1230782A4" modified="2011-01-11" version="1">MNNLTPMIIVMTMVMSVGLVTGDNRDTAQKFGCPHDQVFGHCRWIETNDNGFIKWYVASAPRYGPGYSCRSIDTAYCCDDKKETHAILGNGIKARNDVADFVNGNQCVAAKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KN72</accession>
    <name>E3KN72_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_11037</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EFP85708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330127.1">
        <property type="nucleotide sequence ID" value="XM_003330079.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KN72"/>
    <dbReference type="GeneID" id="10544274"/>
    <dbReference type="KEGG" id="pgr:PGTG_11037"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11037"/>
    <dbReference type="eggNOG" id="ENOG502SQHU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_128330_0_0_1"/>
    <dbReference type="InParanoid" id="E3KN72"/>
    <dbReference type="OrthoDB" id="2502482at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP85708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="122" mass="13244" checksum="881BFAF0D4E16A65" modified="2011-01-11" version="1">MRENSVRLNLGVDPYGTATLTHAQSVAQGSYTVPYVLSSGVNTNILLITMDEDSSLKGGVAPSLYFAALLEFPTNTAKSFQAHLQVASQAQKDHPFKMWCSPKIWSLDYDGQTELGSLEFGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNJ9</accession>
    <name>E3KNJ9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DDE-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11630</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178297">
        <property type="protein sequence ID" value="EFP85874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330293.1">
        <property type="nucleotide sequence ID" value="XM_003330245.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNJ9"/>
    <dbReference type="GeneID" id="10544456"/>
    <dbReference type="KEGG" id="pgr:PGTG_11630"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11630"/>
    <dbReference type="eggNOG" id="ENOG502RT6R">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005726_6_0_1"/>
    <dbReference type="InParanoid" id="E3KNJ9"/>
    <dbReference type="OrthoDB" id="2505908at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35871:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35871">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="155"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="772" mass="87532" checksum="B1DD591AA5402A4B" modified="2011-01-11" version="1">MTTSHVSKAAKGQQRRRRREKSEKDAPVPKAAMKKTKKTKPRKKLNPPGTKLVPIQINSDADDDELQLLNTRPVIDIPKSVQTSTTDHELEEFQEEEIEARNQANDLVQYFNLAINDETSDDESGDDDPLEELWPIFSGCLSSSENSVKKRVLKSGKNGYQKPLSNPDSSSQKLLPAPIPKQTKHDRKKRQIEAIGANNQMFKNYFIRLEPVKNATVNPAIDPDLESASLQDSFLNQNQPVTSKSLDSHQKETNDESLNARFTSILENRLSAIKAPNSASISEKARAEWEELSSALNTATLRYKQKEKKEKKFKFPQAMMNNLYLFNNLRLEYTLNGTKSPSSTASLKAAQSAIKQLPMGTVPPKNRSGIYLSRLISRQATHVINNKHLSEVKRGNRKTHASLLDNAELRKTLFTWAASQVPGHVTPITFRQYVINTIFPKFNIEKSISRNAATRWMIKLGYRPQEHRKTLYFDGHERSDVIEARKKYIEDLENYRKRSRIYGGDNLDTASQVDPEVLGEMKETVFIFHDESTIHAKERPKSSWLLPGTQEIRSKNVGRLIHISDFILETTGRLKLSEAQFRDNKIESDDAAMVIYPGSTGDKWWDMEQLCHQVSKKAIPIFEILHPHSQAVFIFDCSSAHGAYSKSALRVQNMNLNHGGKQSRLRDSVIPTDDPLIPEHLRGLPQSFCYDAAHPDPKRAGQPKGIQAILEERGLWQHYTALQIQAGKRPLKLRCDTCVTSNMRKDALCRSAKLIQQAEDSGYFLSQEQCVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNN1</accession>
    <name>E3KNN1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_11662</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178297">
        <property type="protein sequence ID" value="EFP85906.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330325.2">
        <property type="nucleotide sequence ID" value="XM_003330277.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNN1"/>
    <dbReference type="STRING" id="418459.E3KNN1"/>
    <dbReference type="GeneID" id="10544488"/>
    <dbReference type="KEGG" id="pgr:PGTG_11662"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11662"/>
    <dbReference type="HOGENOM" id="CLU_040578_0_1_1"/>
    <dbReference type="InParanoid" id="E3KNN1"/>
    <dbReference type="OrthoDB" id="2142040at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34706">
        <property type="entry name" value="SLR1338 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34706:SF1">
        <property type="entry name" value="VWFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP85906.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="221" mass="24753" checksum="42B633A32153535D" modified="2012-04-18" version="2">MRASELKAKTPTLGANDFQIHGDENLLSTLKRFKTIFLLDDSASMASNGHWEKAHNSNTHFKNIKGASSLLRKFKTIQPTGDSTPTETKVEGILRPYIEILEMQKSRNQLLPKPLNLVIITDGIPDDIDSFISIISHVSSKLDAGHFPLNQVGIQFVQIGDEKRVSRVLKILDNHLQKRYGVSRDMIDTTQFSGITEKAFIVKCLLGGINRRVDRMQVPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPD6</accession>
    <name>E3KPD6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_12117</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178298">
        <property type="protein sequence ID" value="EFP86161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330580.1">
        <property type="nucleotide sequence ID" value="XM_003330532.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KPD6"/>
    <dbReference type="FunCoup" id="E3KPD6">
        <property type="interactions" value="146"/>
    </dbReference>
    <dbReference type="GeneID" id="10540170"/>
    <dbReference type="KEGG" id="pgr:PGTG_12117"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12117"/>
    <dbReference type="eggNOG" id="KOG3750">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048143_1_0_1"/>
    <dbReference type="InParanoid" id="E3KPD6"/>
    <dbReference type="OMA" id="CERIHAS"/>
    <dbReference type="OrthoDB" id="5579088at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010945">
        <property type="term" value="F:coenzyme A diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034389">
        <property type="term" value="P:lipid droplet organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019915">
        <property type="term" value="P:lipid storage"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019388">
        <property type="entry name" value="FIT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23129">
        <property type="entry name" value="ACYL-COENZYME A DIPHOSPHATASE FITM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23129:SF0">
        <property type="entry name" value="ACYL-COENZYME A DIPHOSPHATASE FITM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10261">
        <property type="entry name" value="FIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="40"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="115"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="253"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="306"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="336"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP86161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="358" mass="40935" checksum="92738C950DC77B52" modified="2011-01-11" version="1">MSSPSLRSRNNTTATAREQPMNIKETQNNSSLVNPANAKWLILIILFLTTTIGTYRSFSIYSRDNPDIPIHQLTRIPLSSLLSTHTTDIRSTTTTLPYFANKSNFLNQKFVKLGWAWTTFVVWFHAIAIATCYFGPPHPNQQQQQTQNSRSHPSSHVPRILSLHLLATLLWIFLTQWCFGSSLIERVLILSGAKCIPSLKPFHSSETTPTTPTTLENLYCQHRWGRNLPGLDNFILSTYKPYWSEGIDVSGHVFLLTFSILLIVSSLSPSLIHLWRTTTKKVNNQNNNKEEEKDQQDETVPRNYRLAIYANLGLLFVWWWMLLMTSLYFHGPLEKFSGFVLAGLAWFFVETSVARLLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPW4</accession>
    <name>E3KPW4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Yeast cell wall synthesis Kre9/Knh1-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_12305</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178299">
        <property type="protein sequence ID" value="EFP86349.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330768.2">
        <property type="nucleotide sequence ID" value="XM_003330720.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KPW4"/>
    <dbReference type="STRING" id="418459.E3KPW4"/>
    <dbReference type="GeneID" id="10540363"/>
    <dbReference type="KEGG" id="pgr:PGTG_12305"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12305"/>
    <dbReference type="eggNOG" id="ENOG502RDM2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109939_0_0_1"/>
    <dbReference type="InParanoid" id="E3KPW4"/>
    <dbReference type="OrthoDB" id="2432613at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018466">
        <property type="entry name" value="Kre9/Knh1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052982">
        <property type="entry name" value="SRP1/TIP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40633:SF1">
        <property type="entry name" value="GPI ANCHORED SERINE-THREONINE RICH PROTEIN (AFU_ORTHOLOGUE AFUA_1G03630)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40633">
        <property type="entry name" value="MATRIX PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G05410)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10342">
        <property type="entry name" value="Kre9_KNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172765" description="Yeast cell wall synthesis Kre9/Knh1-like N-terminal domain-containing protein" evidence="2">
        <location>
            <begin position="19"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="Yeast cell wall synthesis Kre9/Knh1-like N-terminal" evidence="3">
        <location>
            <begin position="25"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF10342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP86349.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="146" mass="15978" checksum="687E2D0FEB7014C7" modified="2012-04-18" version="2">MFNLIAVVYLALLGTAYSAVTPTAPGPKEVFTARQQCKLSWSLDKTNKWKSFSVDLMSGSNLNMNVVTNVFKGMDGTTGKTSHVWKCPEVDPCSAIYFYQFNQAGEDPAWTTRFTIACPDGKIIPPANATQPHGEAIPWGVGHLKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KR48</accession>
    <name>E3KR48_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_13155</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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                <person name="Martin F."/>
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            <dbReference type="PubMed" id="21536894"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
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    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
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    </reference>
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        <property type="entry name" value="VWFA DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87333.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="284" mass="30980" checksum="861A6053EAE43EEE" modified="2012-04-18" version="2" fragment="single">MCNIGDGLSNVCSKPPDKMISRKADLPTCPRVDVGWEATDLAPNTKRKEAQFKATRKERVLNEDEDDPEGSWEKQKGPQNQKTSCIGSSASINRGGVGGVSESSFGSHYRRPSEMATFPHMKRAPLAWPALVATIVLSVLVSERAAYAASTAKPPSPANANPEFVTKSLTSSDNFLHFCAGAKLTNGAQVHEGSCNPTPMGFIPSVENMPSVRIVEPSSEAVLPANQDFEVAIYAHKIQLGFFTPPASTYYQAPQQLNKKGIDQRTHPSGHPKIRRQTRLRRPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3KU32</accession>
    <name>E3KU32_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">glycerol kinase</fullName>
            <ecNumber evidence="3">2.7.1.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ATP:glycerol 3-phosphotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_14522</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the FGGY kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.30" evidence="3"/>
    <dbReference type="EMBL" id="DS178309">
        <property type="protein sequence ID" value="EFP87807.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332226.2">
        <property type="nucleotide sequence ID" value="XM_003332178.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KU32"/>
    <dbReference type="STRING" id="418459.E3KU32"/>
    <dbReference type="GeneID" id="10534705"/>
    <dbReference type="KEGG" id="pgr:PGTG_14522"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14522"/>
    <dbReference type="eggNOG" id="KOG2517">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009281_2_2_1"/>
    <dbReference type="InParanoid" id="E3KU32"/>
    <dbReference type="OrthoDB" id="5422795at2759"/>
    <dbReference type="UniPathway" id="UPA00618">
        <property type="reaction ID" value="UER00672"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004370">
        <property type="term" value="F:glycerol kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019563">
        <property type="term" value="P:glycerol catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006071">
        <property type="term" value="P:glycerol metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046167">
        <property type="term" value="P:glycerol-3-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07792">
        <property type="entry name" value="ASKHA_NBD_FGGY_GK1-3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000086">
        <property type="entry name" value="Glycerol kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018483">
        <property type="entry name" value="Carb_kinase_FGGY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018485">
        <property type="entry name" value="FGGY_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018484">
        <property type="entry name" value="FGGY_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042018">
        <property type="entry name" value="GK1-3_metazoan-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196:SF69">
        <property type="entry name" value="GLYCEROL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10196">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02782">
        <property type="entry name" value="FGGY_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00370">
        <property type="entry name" value="FGGY_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00933">
        <property type="entry name" value="FGGY_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00445">
        <property type="entry name" value="FGGY_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0319">Glycerol metabolism</keyword>
    <keyword evidence="6 10 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase FGGY N-terminal" evidence="12">
        <location>
            <begin position="6"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="Carbohydrate kinase FGGY C-terminal" evidence="13">
        <location>
            <begin position="277"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="417"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="417"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="434"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00043149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EFP87807.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="650" mass="70708" checksum="72C6ADAFE3736E26" modified="2012-04-18" version="2">MPEFVGAIDCGTTSARFFIFDQYAKVIASHQAEYKQIYPQPGWHEQDPRDWVQAIDTCITHAIADFKKMGHQITDLKTMGITNQRETTIVWDKTTGQSLYNAIAWSDSRTTPIVHRFENKASQWGQGADQLKGKTGLPLSNYFTAIKLRWLIDNVPAVQAAMDSDNILVGTVDTYLLWHYTGGINGGSYYTDVTNASRTMLMDLETLDWCPELMNFFGFNSAQLIPLLPKIVSNSENLGSVPSTHPDIPGLLISGLIGDQQSSLVGNQCFSAGESKNTYGTGCFMLYNAGTEPVYSSHGLITTPGYRLGPDGPTTYALEGAVAVAGSSIQWLKNNLGLISSAGEVGTLAASVDDTGGVYFVTGFSGLFAPYWDDSATGLMIGLTGFTTKAHIARATLEATCFQTKAILDAMALDQGLSSSRHTSPPNSAPPGGSFFSHNHSASTSSTSSTFSTYGMPQTPTAKRNAPTRPRLQTRESLGVYDHTLSGPLKSLKVDGGMSASDTLLQLQADILGVVVERSEMKETTALGAALLAGHAIGLFGWDLNRPETLFATHSAGKTIFRPSIPKTKREQRYRGWNRAVTRAKGWLENDDIDEEETIPDCVEIVDHDQVMLVEGLSNVYFGEEGQPVFRREHEQTIVLDPLSKRPLIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3KU98</accession>
    <name>E3KU98_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Wax synthase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_14588</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Secondary metabolite biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the wax synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178309">
        <property type="protein sequence ID" value="EFP87873.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332292.2">
        <property type="nucleotide sequence ID" value="XM_003332244.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KU98"/>
    <dbReference type="GeneID" id="10534852"/>
    <dbReference type="KEGG" id="pgr:PGTG_14588"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14588"/>
    <dbReference type="eggNOG" id="ENOG502SAAK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_633232_0_0_1"/>
    <dbReference type="InParanoid" id="E3KU98"/>
    <dbReference type="OrthoDB" id="1077582at2759"/>
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    </dbReference>
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        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="term" value="F:O-acyltransferase activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="Wax_synthase"/>
    </dbReference>
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        <property type="entry name" value="Wax_synthase_dom"/>
    </dbReference>
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        <property type="entry name" value="LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31595:SF57">
        <property type="entry name" value="OS04G0481900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13813">
        <property type="entry name" value="MBOAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="155"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Wax synthase" evidence="10">
        <location>
            <begin position="262"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="30"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="34"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="65"/>
            <end position="76"/>
        </location>
    </feature>
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        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="95"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007282"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF13813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP87873.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="392" mass="43946" checksum="B707992B7FD77D69" modified="2012-04-18" version="2">MPTFHFICLSIIYGLHQGPVFKSEVELFNQQSKDQPEGRDGPHEGEQQAPQVDQDNKSDANHSTNHLRNRKTKSKTRSQFVNTTTGEKQKEPSPAAQTLPVQTTSTKASVEKMPSIGEMVRWISALIMNPRGLICTWSPPESVVPIERNLNLKKFMIKLILGTVMMHFWFQFVCAFGVEVVINSKADMNHFLFERMGLPPLRIIKLVTPFFLTITLGGGAYSGFALAGGLFNLVEIGIISLLRSILPENSTFRPEPVNPSNYPPLFNNPWLRTSLTEFWGKGWQAVFRLHFLFCGAQPMYKIFHRYGPTVGKLAAVMGAMGLSAAMHEFCLVSVSRIDPTFSSFRMFLSQGIGIALEAVFKQVTGHKVSGPLGWSWTFIYMILNGKPMVDAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3KUF6</accession>
    <name>E3KUF6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13853</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178310">
        <property type="protein sequence ID" value="EFP88049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332468.1">
        <property type="nucleotide sequence ID" value="XM_003332420.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KUF6"/>
    <dbReference type="STRING" id="418459.E3KUF6"/>
    <dbReference type="GeneID" id="10535188"/>
    <dbReference type="KEGG" id="pgr:PGTG_13853"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13853"/>
    <dbReference type="HOGENOM" id="CLU_019528_0_0_1"/>
    <dbReference type="InParanoid" id="E3KUF6"/>
    <dbReference type="OMA" id="WTCTARE"/>
    <dbReference type="OrthoDB" id="2507007at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00084">
        <property type="entry name" value="HMG-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="181"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="603" mass="66567" checksum="6D31564801A417C6" modified="2011-01-11" version="1">MSYSPLNNNVNPRQQVNLHQHGQYPSLSNLVPDTNQNQQQFIQLNGHYGQHSNQSIYDFGDDGLQLLPSEQIYVLPNLQQPTVVQFSQSQQTTSSSSNRAYIPQSAPTQGYFETSTSKQPTYTPLGTQHSPEAQPTPHQGHPQQNPAPVPTPLPPVKPVKKPRKKTIRQYAEANPNPNPAPAATSTPQTPAALSIATPAPDPTTRESVARFLAVPNSNTVPSVRVPPTLESYASKTIDELRAIALAKSKKVMTRDDELYFFQYHELQKTELTLAAINRGVSMSMVESLMGRKQAMREASGWNNFQTKNGHLFEGKGKGVKDSGVMKLLSTKWNGLTPEERAAYQTKNLPHIVDQDETTMDASTDAPDLAMVTGLRAHSKSLKLAEKRVDNFMTEWNKKAVHIAASNHCELVMFAVSNHLHSHNFQLAHSTPGAADFVKQIYEADGIRNYQSRIQSYCTGAEINSISAAVTKTKNKKGVNDNVVAQARAKLAEFVSRETGGRKTSWSWQGCDKALGRLGYKLIFLPGALSNPDWIKSPSSRFLNNEAQLIMDDIDQDLIRIVKDTDALMGHIRQPAGPVKRKTLHISSHEPNNTNSTNALPTSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVA5</accession>
    <name>E3KVA5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_14284</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC6 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178312">
        <property type="protein sequence ID" value="EFP88200.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332619.2">
        <property type="nucleotide sequence ID" value="XM_003332571.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KVA5"/>
    <dbReference type="FunCoup" id="E3KVA5">
        <property type="interactions" value="111"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KVA5"/>
    <dbReference type="GeneID" id="10535508"/>
    <dbReference type="KEGG" id="pgr:PGTG_14284"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14284"/>
    <dbReference type="eggNOG" id="KOG2286">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011776_2_0_1"/>
    <dbReference type="InParanoid" id="E3KVA5"/>
    <dbReference type="OrthoDB" id="190098at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051601">
        <property type="term" value="P:exocyst localization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.357.50:FF:000006">
        <property type="entry name" value="Exocyst complex component sec6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.70">
        <property type="entry name" value="Exocyst complex component Sec6, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010326">
        <property type="entry name" value="EXOC3/Sec6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042532">
        <property type="entry name" value="EXOC3/Sec6_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292:SF1">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21292">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT SEC6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06046">
        <property type="entry name" value="Sec6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="43"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP88200.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="745" mass="84645" checksum="815F4FB2071E85F5" modified="2012-04-18" version="2">MDKDITAARAVAELFKSPDDLVKLAQIRKRLQREQAGIDAKLKQGAKEQLDATREAMSKLRESKNQIEAIKEEMIAVEKACEDPRVRVDGFGKIASVSKIHRNFVATAKMVEQLRDMDYKIDRIEKLLAKDRASPLGDAPNLIAIHYTLSEMETFRNETVLQANREGNSETVRTLAGYFERLAGIIEAFESHYLHLAGNLLDIVRKGHATVAIKIAKIAEIEGQRDERAIAIRLVKRQNKDIGARFQSVRAEARVIKHYRAKVMDAIRTSAKTQIEKQFSKYQDNPSGFFEGENFDWYYQDLLLVEEMLVDKFPADWKIYPAYIKAYHKALYDFTKTYSSSGDAEAGALLALTTFTKEYKKNMTKELDIPPELTDPPLLDDNIQSLVDEYLKLISKKMQEWTQNLMKSETQLFLERTEPPEEDADQMYTMQASGIMFQMVNAQVDFAIDSGQGAVLSRVVEESAKVMMSTQAQWLSLVKKEFQKQHSSKADDIQGGLVEYTISLANDQVKSADFVEGLMNKLEGLVSEKYRQSISENLSAAMDGYLDVAKNCVQALIEAVFNDLKPAVKMLFGNSWYMEGADEPMILIIETIKDYVVDYQAHLNPNLFELLIDDMVDCFLIAYLNGLRKTSKIRIPAAVDRIRADVKISYSFFVTYKSSTELKAYFKVLEHILGVLSASRTMFFLDWHSFAKEYGPAVVGFTEGLLKARDDLDRTAVNELMETIKKKKSELVEPELPTIFSRLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3KXY5</accession>
    <name>E3KXY5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA replication complex GINS protein PSF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_15027</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Component of the GINS complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GINS1/PSF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178320">
        <property type="protein sequence ID" value="EFP89186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333605.1">
        <property type="nucleotide sequence ID" value="XM_003333557.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KXY5"/>
    <dbReference type="FunCoup" id="E3KXY5">
        <property type="interactions" value="206"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KXY5"/>
    <dbReference type="GeneID" id="10546741"/>
    <dbReference type="KEGG" id="pgr:PGTG_15027"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15027"/>
    <dbReference type="eggNOG" id="KOG3303">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1143019_0_0_1"/>
    <dbReference type="InParanoid" id="E3KXY5"/>
    <dbReference type="OMA" id="CLMAYHN"/>
    <dbReference type="OrthoDB" id="10252587at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000811">
        <property type="term" value="C:GINS complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902983">
        <property type="term" value="P:DNA strand elongation involved in mitotic DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11710">
        <property type="entry name" value="GINS_A_psf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21696">
        <property type="entry name" value="GINS_B_Psf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1030">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036224">
        <property type="entry name" value="GINS_bundle-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005339">
        <property type="entry name" value="GINS_Psf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056783">
        <property type="entry name" value="PSF1_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12914:SF2">
        <property type="entry name" value="DNA REPLICATION COMPLEX GINS PROTEIN PSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12914">
        <property type="entry name" value="PARTNER OF SLD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24997">
        <property type="entry name" value="PSF1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158573">
        <property type="entry name" value="GINS helical bundle-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0235">DNA replication</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA replication complex GINS protein PSF1 C-terminal" evidence="2">
        <location>
            <begin position="192"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU368085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP89186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="243" mass="26794" checksum="AFEC07EB8B199D3B" modified="2011-01-11" version="1">MSYNSSAGTSGVPFGAQNALLTASVRERSTDTIKPFEETLVRTLQRETRAFGDQLTQAAEHFRKQQEALDEEDIQMDPGTICALKMSASTARHNKRVLLAYAMQRRNAILDVYWACSASVPNTLVASSSNVSAEKPEGSSERVSDPISKVLTPVELEYLRAHSTLVHDLAKPYHAYTVNLLGNLARGPSRGLFIQVECMRELGEVAVGDGGGMIKFEVGSRYFVKRSEVEQLIEGGWLMEIPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KY37</accession>
    <name>E3KY37_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15079</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178320">
        <property type="protein sequence ID" value="EFP89238.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333657.2">
        <property type="nucleotide sequence ID" value="XM_003333609.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KY37"/>
    <dbReference type="GeneID" id="10546875"/>
    <dbReference type="KEGG" id="pgr:PGTG_15079"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15079"/>
    <dbReference type="HOGENOM" id="CLU_744224_0_0_1"/>
    <dbReference type="InParanoid" id="E3KY37"/>
    <dbReference type="OrthoDB" id="2507423at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="338"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="367"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89238.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="388" mass="43808" checksum="C53181CD517AA3D3" modified="2012-04-18" version="2">MMLSIRSSSSSDSPPLHPLQSGQLSHHHHLPPLHRSQSGRSASKTPPMPIHPTEHPPPRTIFQCNLNQPLFMHYLPKPAHHRSIISQPHPSSSIPKPPPPSFLSTHSSSLTVLLFDRLSADHRATKPHFNSPNSINLASRSLLLYLQKFIMIPQPTQDQLLLRSFLADIAKSCYWSSLIRPLIFLVISYQPTLRPSDRPLPKLLNPPKLPESSSPPAKLPLRLAAPPAPKRFRAARGFKCSPLVDPDPSILHQHQPSSFHLARSTASRTIPQSALFIPPSRKVISQLEIHRLSARAQSRMQRSPYCPAEDSLDGDQRHQRYYLVNAYREKLLLLYEPSDTPLHPPVRQQFELGSDDDEDEHEHGCNDDDDGLEPLEDLLDWDSSTRQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZA6</accession>
    <name>E3KZA6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Prenyltransferase alpha-alpha toroid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_15594</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein prenyltransferase subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178324">
        <property type="protein sequence ID" value="EFP89631.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334050.2">
        <property type="nucleotide sequence ID" value="XM_003334002.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZA6"/>
    <dbReference type="FunCoup" id="E3KZA6">
        <property type="interactions" value="217"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KZA6"/>
    <dbReference type="GeneID" id="10547685"/>
    <dbReference type="KEGG" id="pgr:PGTG_15594"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15594"/>
    <dbReference type="HOGENOM" id="CLU_871940_0_0_1"/>
    <dbReference type="InParanoid" id="E3KZA6"/>
    <dbReference type="OrthoDB" id="10261146at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005965">
        <property type="term" value="C:protein farnesyltransferase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008318">
        <property type="term" value="F:protein prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000072">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045089">
        <property type="entry name" value="PGGT1B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001330">
        <property type="entry name" value="Prenyltrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774">
        <property type="entry name" value="GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11774:SF6">
        <property type="entry name" value="PROTEIN FARNESYLTRANSFERASE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00432">
        <property type="entry name" value="Prenyltrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Prenyltransferase alpha-alpha toroid" evidence="8">
        <location>
            <begin position="1"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP89631.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="319" mass="35424" checksum="2DB22BA3784FB38D" modified="2012-04-18" version="2">MYEWMLSLKTPNGSFAMHQDGDIDVRGCATALSVATVLNLLTPELVKNLPEYLVSCQTYEGGICADSFFNSMAKPEGTQPEYPNAAPTGEAHGGYSMCALTCDFLLQGLPSLSGSPRLDYDSCLRWAAHMQGLPIEGGGFRGRTNKLVDGCYGWWCGSALTLLQALMSTDLSTGLSPKSFYEQGCSDLLDRQALQEYILLISQDLTPNAKKGGLRDKPCTPPDLYHTYYILGALSIMQHTQKHSFQRIQELKQNFKRPHYSKCIIGDSESESDAFQRMKEIYSRVLGWEVQKGFDHKILTKDYIPTPAEIGTCASNFQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZC6</accession>
    <name>E3KZC6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15614</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178324">
        <property type="protein sequence ID" value="EFP89651.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334070.2">
        <property type="nucleotide sequence ID" value="XM_003334022.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZC6"/>
    <dbReference type="GeneID" id="10547705"/>
    <dbReference type="KEGG" id="pgr:PGTG_15614"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15614"/>
    <dbReference type="HOGENOM" id="CLU_030979_1_0_1"/>
    <dbReference type="InParanoid" id="E3KZC6"/>
    <dbReference type="OrthoDB" id="2513234at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="359"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="410"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89651.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="460" mass="50975" checksum="A84E579990FE2827" modified="2012-04-18" version="2">MVPLTRSPIRTRAAANQSHNRSPSGSDVMPDDLIPPNHEVPPHRELSSNSRAADDPRFRAMVIKEANTRFPPEDRLKEDGSNFQIWLRDVQEFALMTFDDDMYFEADHSHDPMDCVAKAALLLSLHRSIKADLYELSSSFSIMAQIKRRFTTFSCAAQLNKWANLFAIPFDPTTNASSVAAFFRHQIHSLSLILHASISHGTELRQEFDMSINRKLSVRRDIPMSFNDYFNVLSSCRERVRARDAGRHCEPLPSGFASHAHDAQHDIRAASVESHPDNVYVLDGRPSGFKTPTPQSRSCFCCGSSSHLISQCNIAPSNPTTGHPPRQSHHPPGPQPPVAQYQAYYPILAPPFHPYHNAYHHRGAAAPPAPLQPADSYRPSYRSNVNTHRPSAREADATMTDPNAVDASTAAPSFSDLTFSATPEGTPSDALFDTGATHHLTGDREGHPDFSWAELNSGES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3L211</accession>
    <name>E3L211_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_16612</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178335">
        <property type="protein sequence ID" value="EFP90586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003334957.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="10530490"/>
    <dbReference type="KEGG" id="pgr:PGTG_16612"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16612"/>
    <dbReference type="HOGENOM" id="CLU_1876467_0_0_1"/>
    <dbReference type="InParanoid" id="E3L211"/>
    <dbReference type="OrthoDB" id="10387638at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP90586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="136" mass="15431" checksum="A847B9A7049C5752" modified="2011-01-11" version="1">MPTMVHIKVLVKQLPEIGVPTPKFLGLQEDCLWMVLRTLWCSKRYRPPQDLLNSNLTLLKILKLKPSPQNSISAVFSPNPTPHTSCFDHTSHPDLRQGQLLSTFPSTNCHRPAPLEFHNVQPQNGESELQNLQKFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3L2E9</accession>
    <name>E3L2E9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_16762</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178336">
        <property type="protein sequence ID" value="EFP90736.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335155.2">
        <property type="nucleotide sequence ID" value="XM_003335107.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L2E9"/>
    <dbReference type="GeneID" id="10530640"/>
    <dbReference type="KEGG" id="pgr:PGTG_16762"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16762"/>
    <dbReference type="HOGENOM" id="CLU_2672287_0_0_1"/>
    <dbReference type="InParanoid" id="E3L2E9"/>
    <dbReference type="OrthoDB" id="10313981at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP90736.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="75" mass="8409" checksum="EF5FF0D0E0BB9C4A" modified="2012-04-18" version="2">MDLFTQLRATRVERKFPFPCGVYSLLSHYADRSLATLLYWDNGIPRDSGLDYAEPEYSLVTLAVPRLASTADGPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3L2G0</accession>
    <name>E3L2G0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16773</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178336">
        <property type="protein sequence ID" value="EFP90747.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335166.2">
        <property type="nucleotide sequence ID" value="XM_003335118.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L2G0"/>
    <dbReference type="GeneID" id="10530652"/>
    <dbReference type="KEGG" id="pgr:PGTG_16773"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16773"/>
    <dbReference type="HOGENOM" id="CLU_712025_0_0_1"/>
    <dbReference type="InParanoid" id="E3L2G0"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90747.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="388" mass="42442" checksum="19575BF828026462" modified="2012-04-18" version="2">MSEYAPTESESDREASDNHHSPAPRPDHRIHRQTQAQSSQTGPIRTSSQPRGRNSIQSIPPLRGSVAAAAAVGREVRRPTAASPQTARQPRAALIPTGGVAQTPAPVAALRTARLPDPDEVRVTAFAGIPDSAIEMTHLDEPIDDEFVQELEVLFELRRGYAHAGESLAYVAPPRQYAIHLYSQLAILQAIDRTRNTVAPVPDVHPVPEVVAPADQARSFMYAPVFKGFVKRRARELLMTKNLKVYGSDPPRGAPGTIKSLLVLVLDYINEQPANFKRDYLPRGYADGHPGAVSSVDSFVRVQLRKSRGMMPPDKHPGTVWEGDHSSNSHYHFLNDLDAGQFDSSASQQHGCCRRRHSGGPWESDSLEGSPCISSYTHHHAVGRQGSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3L461</accession>
    <name>E3L461_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptide hydrolase</fullName>
            <ecNumber evidence="7">3.4.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_16990</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M28 family. M28B subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.-.-" evidence="7"/>
    <dbReference type="EMBL" id="DS178345">
        <property type="protein sequence ID" value="EFP91336.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335755.2">
        <property type="nucleotide sequence ID" value="XM_003335707.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L461"/>
    <dbReference type="STRING" id="418459.E3L461"/>
    <dbReference type="GeneID" id="10529167"/>
    <dbReference type="KEGG" id="pgr:PGTG_16990"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16990"/>
    <dbReference type="HOGENOM" id="CLU_038388_0_0_1"/>
    <dbReference type="InParanoid" id="E3L461"/>
    <dbReference type="OrthoDB" id="10013407at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045175">
        <property type="entry name" value="M28_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007484">
        <property type="entry name" value="Peptidase_M28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147">
        <property type="entry name" value="METALLOPEPTIDASE M28 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12147:SF26">
        <property type="entry name" value="PEPTIDASE M28 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04389">
        <property type="entry name" value="Peptidase_M28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M28" evidence="9">
        <location>
            <begin position="352"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="128"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="143"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP91336.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="587" mass="65610" checksum="3F96EB3ACFF5A491" modified="2012-04-18" version="2">MHARSRGSCKPKEKKEKMKKTKHNCCLLTIFLITRLIISTTAQLQQLSLLPIGTDREGTCHTFIGTTADENHQLFISRPIECPSTDDHADRSKIEITYHPQEDSLIYSINDGQNQEARLASFLSQLDRIGPTSPGRRNPSPQLPSSSSSQVVLEKQQGSHPWIRTLFDEPRKSSTVLIEVPLQSIGLFTDLLKTHELSAISHVPFEEEKQPRSQGSRLSKIGPGFQGSGGLEERLRGLHYKPQIDQILGLIKPDNLLRDIEILSGSDADDHHQRQRSTEDGDWTTRHSFSEGAFKAVQWLQNRYQHLGARCELDHYLPDVAPNLICKLAWSGSDYDRLTQQNRTINHQQLELEAIVLTAHYDSKGSFGSILAPGVDDNASGCAVLLSIAEILQEETERSLSRALLMDDSESRSSGREVELVFVHFSGTEQGLIGSRSVAEKFRDQRTILLLMNLDMISFRVDGEPFQIGLSSLGPSPESLAFIRRISNVYVPELLVGNSTIGVSDETNFYANGFLNSVRIFERIGDAIRNPHHMKSSDRFSLFFEHDHDGDLDDDSDDGKPVGSYDIVQTVSIAKVVLAGVLELLFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3L4D8</accession>
    <name>E3L4D8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Golgi SNAP receptor complex member 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_17369</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport. It belongs to a super-family of proteins called t-SNAREs or soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptor.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of several multiprotein Golgi SNARE complexes.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the GOSR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178346">
        <property type="protein sequence ID" value="EFP91413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335832.1">
        <property type="nucleotide sequence ID" value="XM_003335784.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L4D8"/>
    <dbReference type="FunCoup" id="E3L4D8">
        <property type="interactions" value="421"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L4D8"/>
    <dbReference type="GeneID" id="10529327"/>
    <dbReference type="KEGG" id="pgr:PGTG_17369"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17369"/>
    <dbReference type="eggNOG" id="KOG3208">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078034_1_1_1"/>
    <dbReference type="InParanoid" id="E3L4D8"/>
    <dbReference type="OMA" id="QAYAVND"/>
    <dbReference type="OrthoDB" id="422156at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005797">
        <property type="term" value="C:Golgi medial cisterna"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048219">
        <property type="term" value="P:inter-Golgi cisterna vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023601">
        <property type="entry name" value="Golgi_SNAP_su1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21094:SF2">
        <property type="entry name" value="GOLGI SNAP RECEPTOR COMPLEX MEMBER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21094">
        <property type="entry name" value="GOS-28 SNARE- RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12352">
        <property type="entry name" value="V-SNARE_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF027109">
        <property type="entry name" value="Golgi_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="7 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR027109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP91413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="235" mass="26755" checksum="AF5D45B40983CBAE" modified="2011-01-11" version="1">MSSHGEVSWETLRRQIRSLENTLESEITTYAKLCTSVSTAYSSNGKLSERTITESREVEERIEDNLKQLSLQVDQIYRLLQTSSAAPTGSMTHACNRHKEVLQEYERDFKRTRTSLRECEQRASLLSSVRSEISSFKSSQIASEQDRHLNDRSSINSSHRLADDVLGQAYETRYQFSNQRRTLFNSNSRMGSVIATVPGVNSLISMINSRRRRDTLILATVAGGCTFMLLLLTFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3L4S1</accession>
    <name>E3L4S1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_17600</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178348">
        <property type="protein sequence ID" value="EFP91546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335965.1">
        <property type="nucleotide sequence ID" value="XM_003335917.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L4S1"/>
    <dbReference type="STRING" id="418459.E3L4S1"/>
    <dbReference type="GeneID" id="10531483"/>
    <dbReference type="KEGG" id="pgr:PGTG_17600"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17600"/>
    <dbReference type="HOGENOM" id="CLU_092886_0_0_1"/>
    <dbReference type="InParanoid" id="E3L4S1"/>
    <dbReference type="OMA" id="AHCDHIN"/>
    <dbReference type="OrthoDB" id="2497424at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266">
        <property type="entry name" value="CHROMOSOME 15, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266:SF1">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP91546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="262" mass="28850" checksum="501397268470E84A" modified="2011-01-11" version="1">MGFFTILVDTTSHVAANFSLGSKFDPSARYDFVGEDQLFAPIYEIASFDAMVPTEPPQSWRELVSPERLFKYGYPIFGAYYRDAIAKEQKPEEIHNAILELAHFKLLGPTEHTQSSRIVTKAQAFAFLGPAIQPRINGAYHLNRELIASHSAICDHISPGCDMVMSNYPSQFTFAAAAMKFLKDERKLIDCIKALTTIVLHGLDAAGGAGELASQIILLCAMQAALPVTSREGIVYGRPVRLANFLKVLTGKEEKEDPGKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3L4Y9</accession>
    <name>E3L4Y9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNA-directed RNA polymerase II subunit rpb7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_17668</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178349">
        <property type="protein sequence ID" value="EFP91614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336033.1">
        <property type="nucleotide sequence ID" value="XM_003335985.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L4Y9"/>
    <dbReference type="FunCoup" id="E3L4Y9">
        <property type="interactions" value="516"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L4Y9"/>
    <dbReference type="GeneID" id="10531551"/>
    <dbReference type="KEGG" id="pgr:PGTG_17668"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17668"/>
    <dbReference type="eggNOG" id="KOG3298">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085878_2_0_1"/>
    <dbReference type="InParanoid" id="E3L4Y9"/>
    <dbReference type="OMA" id="TMRQPGL"/>
    <dbReference type="OrthoDB" id="1162399at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031369">
        <property type="term" value="F:translation initiation factor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060213">
        <property type="term" value="P:positive regulation of nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045948">
        <property type="term" value="P:positive regulation of translational initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04329">
        <property type="entry name" value="RNAP_II_Rpb7_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04462">
        <property type="entry name" value="S1_RNAPII_Rpb7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000043">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.120:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="entry name" value="RNA polymerase Rpb7-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045113">
        <property type="entry name" value="Rpb7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005576">
        <property type="entry name" value="Rpb7-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709:SF4">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II, III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03876">
        <property type="entry name" value="SHS2_Rpb7-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88798">
        <property type="entry name" value="N-terminal, heterodimerisation domain of RBP7 (RpoE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="S1 motif" evidence="6">
        <location>
            <begin position="82"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP91614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="172" mass="18891" checksum="9D2C117A1AD9E2FB" modified="2011-01-11" version="1">MFFIKELSHTINLHPSFFGPRTSKFLEDKLNRDVEGTCTGRFGYIIAVLRTLSISAGTIQPGTGMAEFVMKYSAIVLKPFKGEVVDGIVGQVNKMGFFVEVGPLQAFVSSHLIPSDLKFDPNANPPCFSSDEDQATIEKGTRIRLKIVGTRVDATEIFAIGTIKEDYLGPID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3L555</accession>
    <name>E3L555_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein transport protein SFT2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_17536</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Nonessential protein required for the fusion of transport vesicles derived from the endocytic pathway with the Golgi complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Golgi apparatus membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7 8">Belongs to the SFT2 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178350">
        <property type="protein sequence ID" value="EFP91680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336099.1">
        <property type="nucleotide sequence ID" value="XM_003336051.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L555"/>
    <dbReference type="FunCoup" id="E3L555">
        <property type="interactions" value="207"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L555"/>
    <dbReference type="GeneID" id="10531619"/>
    <dbReference type="KEGG" id="pgr:PGTG_17536"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17536"/>
    <dbReference type="eggNOG" id="KOG2887">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099529_2_0_1"/>
    <dbReference type="InParanoid" id="E3L555"/>
    <dbReference type="OMA" id="IAAIVWK"/>
    <dbReference type="OrthoDB" id="73614at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007305">
        <property type="entry name" value="Vesicle_transpt_Got1/SFT2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011691">
        <property type="entry name" value="Vesicle_transpt_SFT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137:SF6">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23137">
        <property type="entry name" value="VESICLE TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04178">
        <property type="entry name" value="Got1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="40"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="69"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="129"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP91680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="163" mass="17581" checksum="C2E5EF91DF0CDA76" modified="2011-01-11" version="1">MAIPKVFANIDTGKVTNNELLDATQPSAFEFLGLSKKQRLYGFFGCLVGGFAVSIIGSILFVFGSVVSFALLYTLGILISLTGTGFLIGFARQIKTMFKPVRVVATILFLGCVIMVFVAAFAINSDVLVLVFAILTFFAYTWYSLSYIPYARALVSKAVGSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>E3L6B3</accession>
    <name>E3L6B3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Eukaryotic translation initiation factor 3 subunit A</fullName>
            <shortName evidence="7">eIF3a</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Eukaryotic translation initiation factor 3 110 kDa subunit homolog</fullName>
            <shortName evidence="7">eIF3 p110</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Translation initiation factor eIF3, p110 subunit homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">TIF32</name>
        <name evidence="10" type="ORF">PGTG_18178</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the eIF-3 subunit A family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178357">
        <property type="protein sequence ID" value="EFP92088.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336507.2">
        <property type="nucleotide sequence ID" value="XM_003336459.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L6B3"/>
    <dbReference type="FunCoup" id="E3L6B3">
        <property type="interactions" value="711"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L6B3"/>
    <dbReference type="GeneID" id="10532047"/>
    <dbReference type="KEGG" id="pgr:PGTG_18178"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18178"/>
    <dbReference type="eggNOG" id="KOG2072">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002096_2_1_1"/>
    <dbReference type="InParanoid" id="E3L6B3"/>
    <dbReference type="OrthoDB" id="18884at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071540">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3e"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071541">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex, eIF3m"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043614">
        <property type="term" value="C:multi-eIF complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.860:FF:000003">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.10:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.860">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.10">
        <property type="entry name" value="UVR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03000">
        <property type="entry name" value="eIF3a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027512">
        <property type="entry name" value="EIF3A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054711">
        <property type="entry name" value="eIF3a_PCI_TPR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14005:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14005">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3, THETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22591">
        <property type="entry name" value="eIF3a_PCI_TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="PCI" evidence="9">
        <location>
            <begin position="420"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="420"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="934"/>
            <end position="1155"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="699"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="803"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="934"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="995"/>
            <end position="1012"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1013"/>
            <end position="1028"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1042"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1079"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1133"/>
            <end position="1147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP92088.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1155" mass="129679" checksum="124B5669CFFEBAD9" modified="2012-04-18" version="2">MSDTRSDCQKLGSPEIARHQFLLTVLPLPAPPTYNCLLTTNLHLPLKLATGNFFLQQLQLEQLHLQVTLSSTIMAPYTKPETVLRRSEELLSVNQPMSALASISEIFSSKRFRQTPLSSLEPIMLRFIDLCVLLRKTRNVKEGLHMYKNVAQNTSVSSVEMVVQHFITKSKEKLDEALAKVDEIEGPLVAEGADQENGAALVDDLEATETPESLLMSTVSEEKSRDRTYRELVTPWLRSLWEAYRTALDILRNNSRLENFYQQIATEAFEFCLTHTRKTEFRRLAETLRSNLASSQKYTNQAHSINLSDPDVLQRHLETRFQQLNTSVKLELWQESFRTAEDINGLIGLSKKVPKNHVMSAFYEKMIRVFGVGENHLFHAAAYNKYFTIQANVAADQPDKLKKLSGLVLLSALAVPVVGSSTSTNEPQRKMRDNEEDSNSLNKTKLGRLASLLGLTSLPTRANLLKDALMRGVLKKSSPELHSLYEILEVDFHPLSITSKIQPILQQLSEDEETKRYVEPLKEVVLTRLFQQLSQVYDSLKLNRVIKLASFGDSEPENLKITRIRVEKFLMEACKRGELEVTLDHSSQLIKFTDRMFENETNSQISSTQLPTSNIPSLLSFNKDQDLVPTQSLAKSGVLQPNSASLLRTHLTRLASALTVSLNHIMPVLSTINETATPDLHLVKSVALQALQIDGPKQRKMLQKRKVTIEERKRKVEELRQKQDIEEAKAKALRIIQIQEEQQIRLQKQNKEREIKKLKDEADKIRAAEAEKVAMALAAQAGLNVDIKNLKGVDTNTIIQMGVEQIEKEKKELAAKMKTVNKRLDHTERAMRREEIPLLAEDYKLQQIRDETNMKKLQEELVEGLQTKHANEVTIKHKLQKMMPDFLKFKERIANQRGHDYKKAKEESLIKIEQAKIERRAQVVKERKLAKAKAAEELKMKEEEERLEEERKKKQAEIDEQERILKAEAEAKAKAMREEREKERAAAAEMAAKQAQREAEAMAKRENKKLAERAAAPPSAGPAAPTAEPWRRTGPGIGAGAEAGAIVSGVAASAAEARPPVVAELKRPTILPGATKAGGWRERLAMKGGEESESTSPVGGLSRVSSASGTRRDNLPPPSTGTGGSGNAYKPPQNRTEPGSGANSRTPSGRGASRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3L6W5</accession>
    <name>E3L6W5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_17964</name>
        <name evidence="4" type="ORF">PGTG_19450</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178362">
        <property type="protein sequence ID" value="EFP92290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="DS178385">
        <property type="protein sequence ID" value="EFP93211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336709.1">
        <property type="nucleotide sequence ID" value="XM_003336661.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337630.1">
        <property type="nucleotide sequence ID" value="XM_003337582.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L6W5"/>
    <dbReference type="GeneID" id="10538008"/>
    <dbReference type="GeneID" id="10543362"/>
    <dbReference type="KEGG" id="pgr:PGTG_17964"/>
    <dbReference type="KEGG" id="pgr:PGTG_19450"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17964"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19450"/>
    <dbReference type="HOGENOM" id="CLU_1027241_0_0_1"/>
    <dbReference type="InParanoid" id="E3L6W5"/>
    <dbReference type="OrthoDB" id="2508958at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5010113593" evidence="2">
        <location>
            <begin position="23"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="249"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP92290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP93211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="271" mass="30505" checksum="6E8731BFECD940E2" modified="2011-01-11" version="1">MICSTVCIIILALKISIAAVEAVMEDVPHISPYCTAGVELKTIPGYCGCGEKIDIKFMYHQCISPKCHASTKPIQVHSGVCGYNLPECTAVGTSTSTTGNMKPGIEMWQMEEPDHSSSHAIAQWEEVISRWKPKINPELQDWESGSQFDSSSLLDNENPTYGPWENDPMHWHQKDCKSEILWLDQLVGRCSCGEFQTRRYSRIFCMSCKSAHPLSYTSYKCKNCRKMAPVPPQNPDWADWTRWLQYINSTPNSSPPSSPLHANRPSSPNMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3L711</accession>
    <name>E3L711_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_18010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178362">
        <property type="protein sequence ID" value="EFP92336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336755.1">
        <property type="nucleotide sequence ID" value="XM_003336707.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L711"/>
    <dbReference type="STRING" id="418459.E3L711"/>
    <dbReference type="GeneID" id="10537998"/>
    <dbReference type="KEGG" id="pgr:PGTG_18010"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18010"/>
    <dbReference type="HOGENOM" id="CLU_062071_0_0_1"/>
    <dbReference type="InParanoid" id="E3L711"/>
    <dbReference type="OMA" id="HAAQCIS"/>
    <dbReference type="OrthoDB" id="2496085at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266">
        <property type="entry name" value="CHROMOSOME 15, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266:SF1">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP92336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="445" mass="49776" checksum="04D9AB5456B9C2F4" modified="2011-01-11" version="1">MDPPADWHQLQSAFRLLSYGSPFWRVYADEGKETGLTDRDIIKGLSEHALQKLLSTDCKPDTLTNAQAMALLGSTIQPRLNRASDLEAKLVSSHASTCMNISTPQLILTSQYPSQFTLSSAANQYLASDEASLIRCIQVLASINRQSLIGPGDVGELVSRIILLRAMQKTMQKTQPAADTETHSENIMMPFGHSVRLVDFLQTLTGWNKNDLKLGSIDKENQEELLTRGQVFWNHFISIDHTPTSAGLLRKLYRGLAVHCKPNQASFDQLFTIYLQSSPTEALDERNVTFCGIQVKNLKQNDNIKEQASKWTPKDAKMKLQGRNPYLVLYFCLNDFRPKKPRLHPKKLKSTSSATSITLETPTPLPKITHIPDGKLSKEEARRRASLAFYGLDAFPFLSEKLVAVLKELLDSDPPVLQLPENPSELDCEYVYGASPEVFSLDKAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3L7E4</accession>
    <name>E3L7E4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18294</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178365">
        <property type="protein sequence ID" value="EFP92469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336888.1">
        <property type="nucleotide sequence ID" value="XM_003336840.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L7E4"/>
    <dbReference type="GeneID" id="10538462"/>
    <dbReference type="KEGG" id="pgr:PGTG_18294"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18294"/>
    <dbReference type="HOGENOM" id="CLU_1468901_0_0_1"/>
    <dbReference type="InParanoid" id="E3L7E4"/>
    <dbReference type="OrthoDB" id="10406262at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="184" mass="20297" checksum="E8D8B15F7972E3EE" modified="2011-01-11" version="1">MAFNFMILTMNNTQSHCATTPIFSHRHPVGPSDDMTARTDIEFFFRCAEKLKKPTPIQRDCSHMGISSKVKTTPSFFFPSYGTSISAPSQEPAACICNTHPISSLASDDFQASLDEGQRLHSKCATGLQPHISTSWTRRMVNEPAVYICNGHAISQLVPDDSQSLDDVQRLPSKSATRPPTSSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3L7H1</accession>
    <name>E3L7H1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_18321</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178365">
        <property type="protein sequence ID" value="EFP92496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336915.1">
        <property type="nucleotide sequence ID" value="XM_003336867.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L7H1"/>
    <dbReference type="STRING" id="418459.E3L7H1"/>
    <dbReference type="GeneID" id="10538491"/>
    <dbReference type="KEGG" id="pgr:PGTG_18321"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18321"/>
    <dbReference type="HOGENOM" id="CLU_735961_0_0_1"/>
    <dbReference type="InParanoid" id="E3L7H1"/>
    <dbReference type="OMA" id="VEHHNIG"/>
    <dbReference type="OrthoDB" id="76215at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024752">
        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46929">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46929:SF3">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12776">
        <property type="entry name" value="Myb_DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like" evidence="2">
        <location>
            <begin position="102"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="274"/>
        </location>
    </feature>
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        <location>
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            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP92496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="376" mass="42055" checksum="00A8C3A2FE8C12BC" modified="2011-01-11" version="1">MSGLQIQSTEVRQDRSLPSISHMDWAASQSLPPLEYLQWGNFHPNLHSSYPPAWSSVESIQTNLAPLNPRETPAPTSTANQPCSDSGDSETPRGTTRPRTLQWTSVMEKAAIQLYYDAALEGRRLDNGFKSDVHQHVVNKLNEQFPGAEFTISKCKSKLSQSFKKEYDAFLACRNASGFGWDEIRCEVTASNDVWEHFLKSHPQARRFRNQPFPEWEQLQVIFGASATGDAAKSLTQHAKEGTVRDKPADKSSSSENDEGEGRARSKAPARKRIRQTTGAAFSSAIHDLIEAFAPQSSKNLTNPEPAAALQTTERLTDEAVDLFQSTMAPQLAWSDLVVGFSVLEKLSKARMYLKLDNMYKNQWLSYEIKKDAQTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8K4</accession>
    <name>E3L8K4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18797</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178375">
        <property type="protein sequence ID" value="EFP92879.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337298.2">
        <property type="nucleotide sequence ID" value="XM_003337250.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L8K4"/>
    <dbReference type="GeneID" id="10542686"/>
    <dbReference type="KEGG" id="pgr:PGTG_18797"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18797"/>
    <dbReference type="HOGENOM" id="CLU_2387242_0_0_1"/>
    <dbReference type="InParanoid" id="E3L8K4"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92879.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="94" mass="10561" checksum="85FEA01FBF28C47E" modified="2012-04-18" version="2">MAISNRRDRCSSFSCHWENVPLGWHNTPPLIHQSNNIYSNVSRRAKTGSSDVMLPSRRRDSSVSFGHLLVLLLSFSDWVLLVGLLPPSSESQNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3L9L1</accession>
    <name>E3L9L1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19182</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178386">
        <property type="protein sequence ID" value="EFP93236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337655.1">
        <property type="nucleotide sequence ID" value="XM_003337607.1"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L9L1"/>
    <dbReference type="GeneID" id="10543468"/>
    <dbReference type="KEGG" id="pgr:PGTG_19182"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19182"/>
    <dbReference type="HOGENOM" id="CLU_252092_0_0_1"/>
    <dbReference type="InParanoid" id="E3L9L1"/>
    <dbReference type="OMA" id="EEDTQYG"/>
    <dbReference type="OrthoDB" id="2503928at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="602"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="760"/>
            <end position="981"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="996"/>
            <end position="1037"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1126"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="223"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="390"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="429"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="490"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="656"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="674"/>
            <end position="683"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="685"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="723"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="837"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1019"/>
            <end position="1033"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1416" mass="157579" checksum="BD67ED62E1AC5554" modified="2011-01-11" version="1">MKRPAEDRVPLWDDPNQLTIPRLRHTLSVYKISIDQGAPRTKLFQQYVRLARIQSPDSVKFWIGEHSLTGPLPAPDALVVSQIKIFLALHKIDYPAQAKRGHLVHLYQSITPAAPPRRSLIPSSPATSVTVSVVIPPHPEHKKRKANLRQKSKSSPPKPLPKSNALWLVPSHIGKPGIEKLPSHGRSKRIKSAARSETFFAPYSRREHAEDEQPSSARPRKVSSTSRRSSTTGASDPGSLSHNSESSQHYTLRRSPRLRAAHEDSRMLAELPSHELSPSSVEPTQSSRNEAHDNESRQYGSDDYSDMPTRRGDVAETSTRASSPTPSVSLADSPIHPVPSRESSQDRHSPTEEEASESPFEAPLTPQQPTQDGPDSPFEAPGSLISPFGRSSVQSSQRSTQEEGPESPFEAASTSRFFEEGAQPPTADLSRRASEEVESSRVQRSSSPVGLGNEAEDNDFQSSEWSSRLADQESEVSTRPSVQAGDPEASSTSPSANHSSESEDHDSEDHDSESSQQSSRPEVSRRRVTHEMTASSRPQPLSGASKKRKSEDPSEWPDASILTRVQLEEYLAAHDVPYDRSTRLARIIGSYNLLRSSLPHLGDLQPFKRPRASQSSPPPSSTGIKRRRTRARVEASSDDDQVGFVYSRPERSRNASIPPQSPSGSGSSDYQPSVAPTETSVGTPSRASISTRSTIRTTRSFPTENPRQKRAKKGNNRPQTSKKAGKRRARASRPKRTRWRVSTHHEADLASDTVNFVPVLPETSKKRKNRSSTPARAKRACPGPDDLPSSEFATHRQASASRRRQPEQTRHNTYTPHPSFRDDGQVDLEDVTPGHTEQSEQSDRLGRPSKRSPPAESSSRSVHTKRTRPNPPDFDWPDLELQQSRPPLRPSTSRREGRVRSDSYAPASSFHDDPSASSFHDGGPTQPHLDHPQETHPLATPRSKRRRPDDSSPSPQRAKRQAPSAACLIPSSAHVRRRRAREQRVRLNSLTIPSIHDDFIVPNPSESGVERWRNSVPPGLSPPSRSPSPPPSANPNLRRCALGECTGMLSELTQAAGRLTDSIVHATNQASLMQSTRLMPINGQAQIPASSSAPTHQDPIRQDNGVEYLSTPATAAEKAVWVRDPANDYPEDEPMSASSASSHDDAVDPCFPYPNGPGHREASASTLKIMWRSMQRCRVVSFRPDFVRAVRDTDNLFLWDLAHSIFIKLVRAQEYPEIDLETCSSAKIYDAILNHAKQLKRSYTEAGWDPRRRDAQAQKKRRQMRTTRLRDARLKFCTTQSFLIPLIPVIDECTSDAESDAEIEGSDMDTGENERITFVTKLPWRHPRIEEAVQLIDQLIERKRKSGSRDFGRSPTYTRFRYGNPRISNRPCPAQLPAHAYCEDWLKTQRIPELTKLHIQTGPSLKGLVKELKKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3L9X1</accession>
    <name>E3L9X1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19301</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178389">
        <property type="protein sequence ID" value="EFP93346.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337765.2">
        <property type="nucleotide sequence ID" value="XM_003337717.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L9X1"/>
    <dbReference type="STRING" id="418459.E3L9X1"/>
    <dbReference type="GeneID" id="10543692"/>
    <dbReference type="KEGG" id="pgr:PGTG_19301"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19301"/>
    <dbReference type="HOGENOM" id="CLU_004591_1_0_1"/>
    <dbReference type="InParanoid" id="E3L9X1"/>
    <dbReference type="OrthoDB" id="2497755at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1095"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1110"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1132"/>
            <end position="1148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93346.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1148" mass="129168" checksum="5B03234E517E161F" modified="2012-04-18" version="2">MRAPGPALDYQFSDGLYLCHLCPRATGTKNWRRHCASVTHQRQVQHRAEARRVSQPVVQETVGAPGDPGEADQHDDHIQEDNAHGERVWERIERFLALDEAEAALDTNTRALDNYLDELESRGAREGGANLGTGTGQGPNWRGLAETALMGFEVDKDDHIVEDPPILRTDKRSDRVNKSPWYPFKSQMDLIASLIVGHTHSMLSRSLYTKIRAILTLCGLRLRAWSTVRTSRERIRGLLGSKIGKAVSVFDVPCYALSAKKILSQELANPRVSKFLDYYPEMTDGLMIKKFSQSGKWLSGMSRAIRPQMCEAHGKHFYIYEPVQLRSHVVVVPVYLFMYQSVLHARCWEILDDHITSTETSVEGNLSTVLNIRMPLDLDFDDARLRSIPVHQFDVEYSGIRWEDGRLLVEAYGTGLSGEVLTFPNPWRAKAKKMILRNVPITLYSDNTSGNVSKQFNKHISFYFTLCGLPPHLSNQEYNCHFLSTSNLASVGEIAEQIVEEMNDMTTEGFEAYDHSIGQKVWVTSSVFCFLADSPMHAEVTCTPNPGASINPCRMCKLSVSMKRLKRTRRFIRAFLGRGFHGNKVRGDPREWEETKSRCRELWEIATTQSWNQFVIKCKEYGLKDTITTRLLTEAKLDSCIKEKMMNMALGDSNRLYSPFLQLIGFDGVRDTPVEVLHVVLLGVVKYLARDLVAGISEGDQKQLVGRLQSFDCAALNISSFKPKYLIKHIKSLVGRDFKILLQAAPFVFGEWMSEEQKPIWFALCKLAPLIFQTCIDDMDAYLIELTQHIDIFLFHIIRSTAQWVNKPKFHMLVHLVECIRRFGPASLFATEKFESYNGVLRKASVHSNKLAPGRDLATSFNDFGSLRFVVSGGIVLDNQTGGPRPIGTSVTSVFDDNPSVQKSMGYNHLACHPDDRRDYPRKARGRVGEKMVSVPPAALLEAHPSAVVKQVGRVDVSEHDRVGSGSFVVVGESPMGELVVGHVDSLWEVSANGRKSLVNVKFILGCINVQHNCHRGECPVELTKEEDMERQATGRKGWQVEHSDQEHYIINSGALRNAVLHRRISGLPMDLPDRHKWTEAIDLGLGIWNTGLDPIQSDDEHFDEYQPGSEDDAEGETDSEFARSSNSSGSDNDEDADGETDSEYDEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3LAL0</accession>
    <name>E3LAL0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19638</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178399">
        <property type="protein sequence ID" value="EFP93585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338004.1">
        <property type="nucleotide sequence ID" value="XM_003337956.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LAL0"/>
    <dbReference type="GeneID" id="10534792"/>
    <dbReference type="KEGG" id="pgr:PGTG_19638"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19638"/>
    <dbReference type="HOGENOM" id="CLU_035345_1_0_1"/>
    <dbReference type="InParanoid" id="E3LAL0"/>
    <dbReference type="OrthoDB" id="2513234at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="390"/>
            <end position="464"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="437"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="487" mass="54446" checksum="5C6498BE26DE5FA2" modified="2011-01-11" version="1">MPFVRHSPVRHQPAMTPGSLEDAGDASFHNDTLLDDQHDGVPPLGPRGNLDDRNVDTRRRHRDHTPLPPPPTLDDQRIMDDQRGINSMVNNAIQEFGTVNFLSADGSNFRAWTRDIEMIALSFLQDKDFFNSPCARPRLEPAGRRILLGVIDSSLKHELYSKESSYKMMEAIKARFSSVSKARMLTKWRELLRIQVDMTSNPASVATQYKAIVDELGDMGVYLAYDDLLPLILHDSIPQGSNLRQEFDRRIDAEFALNGRQVITFEKTWRILSEAICQVRAVDSLDNLQRLPHVMAAEQAPRPREERSASIISHPDNVYALAGNPQQNRRICFRCRSPNHLIGQCTAPDPATRPPNQQPRSQAQHGPIPPGFQAYYPIIAPTGAVPMYYPPRLNQQNPNSIPVQRGRMDSYRPQYGQQSNRPSARAAEPAGVPPSDPSFNNLDVSANPAGDSNPQDALFDTGASHHLTGDNAGVHGFYWGQWNDGDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3NXN5</accession>
    <name>E3NXN5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_20288</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS989956">
        <property type="protein sequence ID" value="EFP94334.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338753.2">
        <property type="nucleotide sequence ID" value="XM_003338705.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NXN5"/>
    <dbReference type="GeneID" id="10527464"/>
    <dbReference type="KEGG" id="pgr:PGTG_20288"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20288"/>
    <dbReference type="HOGENOM" id="CLU_2518771_0_0_1"/>
    <dbReference type="InParanoid" id="E3NXN5"/>
    <dbReference type="OrthoDB" id="10326193at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP94334.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="85" mass="9664" checksum="8F1EAEAC1270B4F1" modified="2012-04-18" version="2" fragment="single">MKLFQLEVESYEALGPGALMKPEEEQRVDLDVKPFSSRSPVLVDGYGTIDLKLNRFQLEVCENFLPIDNDLELKTIQLEVLHNSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>H6QP26</accession>
    <name>H6QP26_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_20725</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EHS63140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890695.1">
        <property type="nucleotide sequence ID" value="XM_003890646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QP26"/>
    <dbReference type="STRING" id="418459.H6QP26"/>
    <dbReference type="GeneID" id="13542589"/>
    <dbReference type="KEGG" id="pgr:PGTG_20725"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20725"/>
    <dbReference type="eggNOG" id="ENOG502S88Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012390_7_0_1"/>
    <dbReference type="InParanoid" id="H6QP26"/>
    <dbReference type="OrthoDB" id="2506822at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125">
        <property type="entry name" value="F21J9.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125:SF3">
        <property type="entry name" value="NO-APICAL-MERISTEM-ASSOCIATED CARBOXY-TERMINAL DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="2">
        <location>
            <begin position="199"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="267"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS63140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="353" mass="39821" checksum="7D29B1391A8CEEEA" modified="2012-04-18" version="1">MPPKRVVLDPLLMEMNQTTPTATTRTMRTTHPDPHATPTGSVPATTLSMPSQLDPHTTPVPQPASSNEITDETDDAPKKKAPKWTIDEDKQLCAAWLNTSRDSIVGTGQKAGTFWERVHQLYTDLVVDYNKENKRSKTIKPLPVQLVNAIECRWGHIMRVCNKFGGCYSQVERRMRSGMSRDDILSEAKELYKSENESAFNLDHCWGILREHPKWQATQQEIDLRQKKSKDPPSSTPATNEASLPSSPQTTTQLDDDDDRSALGSDARPEGNKAAKRKREEDSILQKIIKTQEDLVKISKERSASVQKALEEAAEAKKQEADDRIMAMDLSGMDDEAKAYWQKKRRAILDRPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPA9</accession>
    <name>H6QPA9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20667</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EHS63572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890633.1">
        <property type="nucleotide sequence ID" value="XM_003890584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPA9"/>
    <dbReference type="GeneID" id="13543138"/>
    <dbReference type="KEGG" id="pgr:PGTG_20667"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20667"/>
    <dbReference type="HOGENOM" id="CLU_2016367_0_0_1"/>
    <dbReference type="InParanoid" id="H6QPA9"/>
    <dbReference type="OrthoDB" id="265717at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003606334" evidence="1">
        <location>
            <begin position="19"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="123" mass="13586" checksum="12C21FD093225749" modified="2012-04-18" version="1">MMMKLIVLAVSYLQTIQCMNGGRGGITHELLAASKGKNAAQIAEAPSFTEGDVNLYFTTVRAFTRPAPGSYKDKLDLTIAKPKDDQEGSSEKFVLDTYWPAVQAKLLWEDFNPQIENASRLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQ13</accession>
    <name>H6QQ13_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20959</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EHS64496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890410.1">
        <property type="nucleotide sequence ID" value="XM_003890361.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQ13"/>
    <dbReference type="GeneID" id="13542697"/>
    <dbReference type="KEGG" id="pgr:PGTG_20959"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20959"/>
    <dbReference type="HOGENOM" id="CLU_2639277_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQ13"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="77" mass="8680" checksum="1C91176474FBE9C7" modified="2012-04-18" version="1">MDPTQTLHTKERCYQNSPQSGSCPDCEAQQRSSSISRSSAAIDCREFLSEEIVIAKERISVEVAVSAIVRREYLKKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQC2</accession>
    <name>H6QQC2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21082</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EHS62533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890246.1">
        <property type="nucleotide sequence ID" value="XM_003890197.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQC2"/>
    <dbReference type="GeneID" id="13542239"/>
    <dbReference type="KEGG" id="pgr:PGTG_21082"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21082"/>
    <dbReference type="HOGENOM" id="CLU_2122255_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQC2"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="114" mass="13000" checksum="9022FF7B1027692A" modified="2012-04-18" version="1">MVQPDARSDKTQNSRQPRPREAYLDDRARIYYQASHPALVGPRHPGAPPLAPCRGTSEHQPRRRQEHKEFFEAQAATKKSTKMSEHKCERLHSRELIHSFPGELLLETSSSGTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQD4</accession>
    <name>H6QQD4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_21094</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EHS62546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890259.1">
        <property type="nucleotide sequence ID" value="XM_003890210.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQD4"/>
    <dbReference type="GeneID" id="13542220"/>
    <dbReference type="KEGG" id="pgr:PGTG_21094"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21094"/>
    <dbReference type="eggNOG" id="ENOG502S1WT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003292_0_1_1"/>
    <dbReference type="InParanoid" id="H6QQD4"/>
    <dbReference type="OrthoDB" id="5149081at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052055">
        <property type="entry name" value="Hepadnavirus_pol/RT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050:SF7">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="887"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="930"/>
            <end position="968"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS62546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="984" mass="111917" checksum="0195F82203FBEF65" modified="2012-04-18" version="1">MNLPDKVTCEMNISKWREALQVGNLLPEYQDVIDGFTNGFDQGIPQHIIEGKHWFTPENHKSSLLVKDKIEESISKELEAKRMLGPFSHQQLKETFGFFRSNPLGAVVNGDGQIRPINDLSYPRNDPDIRSVNSYVDKSDFETTWDDFKIVSKFFAENDQKFDLALFDWEKAYRQIPTRQDQWKYLLVHDFDGNLLIDTRITFGGVAGCGSFGRPADAWKLVMKNHFNLVNVFRWVDDNLFVKEVDENLSMREIVSKSTELGVMTNIKKFSDFTAEQKFIGFVWNGHLKTVKLPEGKIEQRLAQIHPFQVKKAMFDYEEAEVLVGRLNHVSYILPHMRCHLCSLYKWLKSWIWRKAKRATPVDVLEDLDVWVNTLNNFEHTRLINWGPPLDVGWVGDASTSFGIGILVGKHWAQFKLIDPQSNQKRILFLETVAIRLGLLMLLKLRSQKGKSLIVWTDNTTTENSINNKKTKDAEANNEWKKIQEVLLRESVNLVARRVKSKDNKADALSRGIRISHRLQFILHQKSMPVTSNDSFLSSIRDFTSRGHLLHTPSRLDQHVLNGWSKSTLKGYNSAVRKFLTFKKTSGHPCFTLPAAEEDIYGFCFWAGKKDSDGGETGVLSVTLKKYLQGLKAWHIFHNSPYPLISEKKVGLLLKASLKVEAQAAKKEPKKPVMIEHLVVLANDLTSGDLEDAAVLDLALVTFWSLARLGEVTHDPSNPGRRILRCDITISNNGLSATIELKESKTALPGESQFLQLKQMPNCLCPVRALTRRISSCGQNNHIFGFHKDGRHIALTKYYAKKRLSAIWAAHGFDGLSGHSFRVGGASFQNALGIPPEQICKVGRWASAIYKLYIRVYSSDDLSTSLETLSRLDLQWRMMPPSRAVNELRRWSLPRPSRAPPENPRDSRGPGRATGGAPNTLDKTSTTRNTYRDGKSVVGLDWERQERIADVSEVQDPERLEPCPELRGQDWPGKARPPREGERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQH9</accession>
    <name>H6QQH9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21187</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EHS62679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890225.1">
        <property type="nucleotide sequence ID" value="XM_003890176.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQH9"/>
    <dbReference type="GeneID" id="13541233"/>
    <dbReference type="KEGG" id="pgr:PGTG_21187"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21187"/>
    <dbReference type="HOGENOM" id="CLU_2528541_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQH9"/>
    <dbReference type="OrthoDB" id="10303512at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS62679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="84" mass="8708" checksum="D1288FC6D2888772" modified="2012-04-18" version="1">MLRARPSMKQITSARVAAFAGIVAWIVSFSTPAASAAQDIVVAPLARAETAAAKSHIGQHQASTDETVCDVCKDAGRAYCNCTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQX6</accession>
    <name>H6QQX6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21263</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EHS62913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890073.1">
        <property type="nucleotide sequence ID" value="XM_003890024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQX6"/>
    <dbReference type="GeneID" id="13541555"/>
    <dbReference type="KEGG" id="pgr:PGTG_21263"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21263"/>
    <dbReference type="HOGENOM" id="CLU_174984_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQX6"/>
    <dbReference type="OrthoDB" id="2505956at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS62913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="79" mass="8695" checksum="DCFD106FE9A8F71B" modified="2012-04-18" version="1">MSNIDMYVTEGSLPNVENQLADKVSMLLDTLIPRLESLERAVSGQVSKTSGATRADSFNTLLVRIEEIAANMARLEKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUI6</accession>
    <name>H6QUI6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22407</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178342">
        <property type="protein sequence ID" value="EHS64698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888833.1">
        <property type="nucleotide sequence ID" value="XM_003888784.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUI6"/>
    <dbReference type="GeneID" id="13542466"/>
    <dbReference type="KEGG" id="pgr:PGTG_22407"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22407"/>
    <dbReference type="HOGENOM" id="CLU_3033451_0_0_1"/>
    <dbReference type="InParanoid" id="H6QUI6"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="55" mass="6450" checksum="6ABAB555B75290E4" modified="2012-04-18" version="1">MELEIPYARRPHCCRGCYGERRVGGTFCGERYRALMSQRRLPLQKSRDVTVVHLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>H6QV11</accession>
    <name>H6QV11_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22599</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178368">
        <property type="protein sequence ID" value="EHS62624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888647.1">
        <property type="nucleotide sequence ID" value="XM_003888598.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QV11"/>
    <dbReference type="GeneID" id="13542274"/>
    <dbReference type="KEGG" id="pgr:PGTG_22599"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22599"/>
    <dbReference type="HOGENOM" id="CLU_2623161_0_0_1"/>
    <dbReference type="InParanoid" id="H6QV11"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="78" mass="8392" checksum="BC51078D45B8F735" modified="2012-04-18" version="1">MALMIRWPACAAPKEQMGGCNSGGTESSEDIRVAGRTWSSNYCASSKWTVTEAYQGTQPTEFSTSAVAGRRDREALNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>H6QVE5</accession>
    <name>H6QVE5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_22720</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178406">
        <property type="protein sequence ID" value="EHS62899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888514.1">
        <property type="nucleotide sequence ID" value="XM_003888465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QVE5"/>
    <dbReference type="GeneID" id="13540828"/>
    <dbReference type="KEGG" id="pgr:PGTG_22720"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22720"/>
    <dbReference type="HOGENOM" id="CLU_026798_0_2_1"/>
    <dbReference type="InParanoid" id="H6QVE5"/>
    <dbReference type="OrthoDB" id="2504339at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="118"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS62899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="474" mass="54286" checksum="79F83FF88DFDEBA6" modified="2012-04-18" version="1">MGKNIRRNNKSRKEAKQRNRNNRQRENNAKLNHEALGITNRSITWHWVRYRPISLYPTIQFVDEKPTRGPTDTELKAAQDEVKSFRLIKTGLNVIRDPTDKKSVIAIIEFTRFEDLTDTEKDDLNFVSTFLHDSKKWISAVRSSSRIWGGLMWAIGWRKSSDTNQIAGRYIKAFNESQMFAYDFHYKKSARLGTIIGNLFKKMAHIPFQKNQDLMKEFNLPDFASLSFNEPPADSTCSPHITFTTNQFFNPPHKDKNDISEYAFVLFLPSSAKDGTLVGSSSGYDISSGQFVFPDHRFGIDFDHQHGVVKMIWQANKYTHCTMPSSPSSQFYRLGLSVQINYSLALTCDKYIKGYYRDLANYFADHLYYLKRCIGSSSSPALSQAPRPRSSIGMSWLTSSSPFGMSTHWRSALVPDSSRSTRTVPDDETSHPPAWQLCWTLIVETTSYKVGIAVRNWVIASDVENNLPQFLQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRF5</accession>
    <name>E3JRF5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_00706</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307756.1">
        <property type="nucleotide sequence ID" value="XM_003307708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JRF5"/>
    <dbReference type="GeneID" id="10546603"/>
    <dbReference type="KEGG" id="pgr:PGTG_00706"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00706"/>
    <dbReference type="HOGENOM" id="CLU_1315956_0_0_1"/>
    <dbReference type="InParanoid" id="E3JRF5"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP74750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="209" mass="23146" checksum="08442063D45DA69D" modified="2011-01-11" version="1">MSLRDVIEDLINPGSSRKLCLVTHIVLEPVDALWKAKAKKGFDPNCTDTIKLKSELQTGQGKVMKGFWVDLVLGRQKELRGAGIVGLIIHKIMLAKSIMVVGFCWEISSNLLEIHVLRELRKGIAGARAEVWRRRFETGVGHIESALWALSSSGESSTSWPKEENNWTEFLEYWSTTCLIGLLVSHGIVVLSGSLNNFPSLVTAPMDSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRM8</accession>
    <name>E3JRM8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_00723</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307773.1">
        <property type="nucleotide sequence ID" value="XM_003307725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JRM8"/>
    <dbReference type="GeneID" id="10546622"/>
    <dbReference type="KEGG" id="pgr:PGTG_00723"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00723"/>
    <dbReference type="HOGENOM" id="CLU_1384765_0_0_1"/>
    <dbReference type="InParanoid" id="E3JRM8"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP74767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="197" mass="23209" checksum="DD8DC5EBE77D14E6" modified="2011-01-11" version="1">MELVFKLEERLSEALKNNDFHQQKSKILLEVKDELTEMMDFLLGPIGVAQDDQRVSNRFTILFLECISNTFGSNYLEMLGFEESDPRTKEVNQKLHYLTQFQRPIMWKLQLEQYCNYLFPGWDEFLKRPKAIDEYFKSLYMGEDSYPRPKTLLEEPHTLNFDCPRYIDDDSLWWSSDGSIGGHLDIGEIGKIIISCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRN2</accession>
    <name>E3JRN2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_00727</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74771.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307777.2">
        <property type="nucleotide sequence ID" value="XM_003307729.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JRN2"/>
    <dbReference type="GeneID" id="10546627"/>
    <dbReference type="KEGG" id="pgr:PGTG_00727"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00727"/>
    <dbReference type="HOGENOM" id="CLU_1116198_0_0_1"/>
    <dbReference type="InParanoid" id="E3JRN2"/>
    <dbReference type="OrthoDB" id="2507653at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171479" evidence="2">
        <location>
            <begin position="17"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="157"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP74771.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="202" mass="20817" checksum="4694598D96B261F1" modified="2012-04-18" version="2">MKFLVLLVSIPALVSSMHIDNSAGLDGGNAKSSSRESTRPQIRAPNAARSTGMANPHSTSLTKIGSSEPSTESPRTLKLASKSTQDGQTTSPLPIPSNQAETSSSKSAGSKSRLRRHISDSRAFESYLIHKRADKVPAKNLLRTFIDDINPSPGSKPAGPTTAGPSAPDSSPSGSSSAAVGSKPTQAVSTAKPPPSKSTKTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>E3JS09</accession>
    <name>E3JS09_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribosomal RNA-processing protein 14/surfeit locus protein 6 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_01427</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SURF6 family.</text>
    </comment>
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        <property type="protein sequence ID" value="EFP74834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003319205.2"/>
    </dbReference>
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    <dbReference type="STRING" id="418459.E3JS09"/>
    <dbReference type="GeneID" id="10546950"/>
    <dbReference type="KEGG" id="pgr:PGTG_01427"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01427"/>
    <dbReference type="eggNOG" id="KOG2885">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_573744_0_0_1"/>
    <dbReference type="InParanoid" id="E3JS09"/>
    <dbReference type="OMA" id="RIETTQK"/>
    <dbReference type="OrthoDB" id="444809at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029190">
        <property type="entry name" value="Rrp14/SURF6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029188">
        <property type="entry name" value="Rrp14_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007019">
        <property type="entry name" value="SURF6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14369">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14369:SF0">
        <property type="entry name" value="SURFEIT LOCUS PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15459">
        <property type="entry name" value="RRP14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04935">
        <property type="entry name" value="SURF6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosomal RNA-processing protein 14 N-terminal" evidence="6">
        <location>
            <begin position="16"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosomal RNA-processing protein 14/surfeit locus protein 6 C-terminal" evidence="5">
        <location>
            <begin position="174"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="46"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="62"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="95"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="145"/>
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        </location>
    </feature>
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        <location>
            <begin position="169"/>
            <end position="200"/>
        </location>
    </feature>
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        <location>
            <begin position="212"/>
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        </location>
    </feature>
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            <begin position="230"/>
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        </location>
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        </location>
    </feature>
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        </location>
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        </location>
    </feature>
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        <location>
            <begin position="325"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="369"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="381"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
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            <begin position="393"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="418"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="434"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF15459"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP74834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="460" mass="51421" checksum="E9497721858E5D0F" modified="2011-01-11" version="1">MDQELPKEMEPLRAAILAHDQQFQELLRMIPPKYYIAQQQFSVTSFQNNSQKASKKNKKNSHHVDPEAKRLQKRAKYDPDTLPTIPQLQGLNKSGCEKLESKEKSSESSQESDEESDGPKDAVSPAPQLSRAELQAKLQKRIETLQGSSQRANPSSENQQSAGVTGKEALLDLERKKRGEMRDRRRKERKEARSKEKQNKLEQSLLKKKKLSGSASSKPSNAPSGSGNRQKGDKTAQDDQPKKDVSKNAAPPSKSSHPNPSPSKAEKPSAGPAPAEGSKSSTKNQAPAQEPDLAFSSLSFDLKQSTGVEGGHSHGQKNPSWTSKKPQEAKTALDRREAYLEKLTPEARERAEESKKWEKANLQASGTKVMDDRKKIEKALKKQTKEKKKKKHQWDERIKVVEKLKEDKQKKRTQNIQARKDQIRDKKNGVKAKTGVKAKGNHPSKNKSKPSSGKKQVKGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3JS20</accession>
    <name>E3JS20_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_01484</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
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                <person name="Yandava C."/>
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                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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                <person name="Martin F."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
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        <source>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP75021.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
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    </evidence>
    <sequence length="255" mass="28038" checksum="1A3110F712814700" modified="2012-04-18" version="2">MVVPFTIHEVAGGPETTSFKQSSTRASIRGDGTALFVQTRDLKTSFTESLGLFTMVGAIFMVSAVAVFWHQYLDRHDIMITAKKSLLCIALHALGASRLVLSAPMQSARAADDVAHLGRQSAGLHGEEIEQVGVGYNAAGQSSSDVQRGIGHEISEDRGNHHTSAKESIDSESDKIGDMDQVIREEYRYISDQDLARLMSSMADLSDSVTQKGLSYLLPRIGHLQKMSNKYTAWIQKLEMNKNIYEKGFVQGIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>E3JSL5</accession>
    <name>E3JSL5_PUCGT</name>
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        <recommendedName>
            <fullName evidence="17">C2 domain-containing protein</fullName>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
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                <person name="Montgomery P."/>
                <person name="Pearson M."/>
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                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
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                <person name="Mago R."/>
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                <person name="Murat C."/>
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                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
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                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
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                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
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                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
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                <person name="Schein J.E."/>
                <person name="Zhong S."/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21676">
        <property type="entry name" value="SMP_Mug190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057349">
        <property type="entry name" value="C2_Mug190_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037767">
        <property type="entry name" value="C2A_Mug190-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037765">
        <property type="entry name" value="C2B_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47348">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 190 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47348:SF3">
        <property type="entry name" value="MEIOTICALLY UP-REGULATED GENE 190 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25331">
        <property type="entry name" value="C2_Mug190_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25669">
        <property type="entry name" value="SMP_MUG190-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="189"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="SMP-LTD" evidence="14">
        <location>
            <begin position="260"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="486"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="668"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="133"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="645"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="704"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1043"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="30"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="47"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="70"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="133"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="648"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="664"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1050"/>
            <end position="1061"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EFP75040.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1142" mass="127421" checksum="D38AD5B18F63714A" modified="2012-04-18" version="2">MDSTHPKMQTSGHHFQENYSARNPVPTIENYEKNIKNLEAKNHENQSIKSTSINTIQHGSENRSPIGDSDGPKKDVDSSDHSEAEKQAIMERMKNKDEKPAKELKLRGQRTVKDPVTNSDVIISDAQFDTNQASRNHALSPPQNSNRTEPSLRISPSPIAPGNICLQPFPPPVPSNLLDEIKKFINKTIYFFLGSLLVMWWTTAFGAGVSTFLWRSCLFGIVGLVSFFQGGLLDRQIEKELDKIRFEMHRQRGIENSPPTPESTEWLNAFISTLMGLLNPDMFLPLADTIEDVMQASLPSFIDAVKVSDLGLGDTPLRILSMRALPDQPGDPEYPRDSWITGDYHQSESEKFREEQSGDYVNYEVAFAYQAQPGQTDKLRAHNIHLMVEFFVGVFDWVHIPVPIWIQIEGLAGTVRLRLQMIQEPPYIRNLTFTMMGVPKVSVSAKPMARSIPNVLDLPVISGFVQSSIAAACAEYVAPKFMTMNIQEMLSGSGVKTKTHALGVIMVTIHHATGLSAQDASGYSDPYIVLAYAKFGKPIYSTRIIGKELNPVFEETAFLLLSEDEIQSKEKLSVMLWDSDERSADDLVGRIELPVSQLIQNPNQLSRRTDKLQGFQDADDMPGQLTWSVGYFSKVDLASYLQKNTAGSSSELSSRPTSTPATPETKDDSKDLTTKVPPNRQMRSGIVSVIIHHINNLERANLKGASGDREGAQGQDTAAPSEQDDNLPSGYAEVIVNDEMIYKTRVKQYTTMPFFEAGTEHFIRDWTETVIRIAVRDSRIREKDPLLGVINIDLEQLFEDSSCSQVTRIFSLQEGVGFGRAQVSVLFESVQLQLPKNLLGWNTATLEITSPIRAELSDELSAYLKKKGQKLKLKTVEDIIKLPLVSHQEIPNTVVWANDSQIPDHDHTLLRVPIYARYKSNITFQIGGDSVISSLLPGESSAHKHIAVLWLHTLEDDVEQQIRLPIVSGPNLTTLRQNFINDQTRAHHNYEVNGYLTFRARVDPGLDPEHARYAQSSVARHTFEAYDNSEGQAEQALRNAHALAHKKNPSGSSDSAASTNSEGHAGTFRLSSIISSKSPEEKAIEAAHKKSLSSRHRGAMQYGPVRTSIWMKEGIGRRARELKNRITNKIEKEPQVATEGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3JSM2</accession>
    <name>E3JSM2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01640</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75047.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319466.2">
        <property type="nucleotide sequence ID" value="XM_003319418.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JSM2"/>
    <dbReference type="GeneID" id="10547409"/>
    <dbReference type="KEGG" id="pgr:PGTG_01640"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01640"/>
    <dbReference type="HOGENOM" id="CLU_573821_0_0_1"/>
    <dbReference type="InParanoid" id="E3JSM2"/>
    <dbReference type="OrthoDB" id="10518062at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="122"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75047.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="476" mass="53000" checksum="CAA86FF21363EC9A" modified="2012-04-18" version="2">MNHYSHPHINPGQSPRQMYTIADANLGNQPSNITPTRNRATPRTQVPGGGQLGPHRMTPTNVRYHPHGAGGTPDQTSQVIPATNPGGHGNRSMDPRTETDLEGPNLGNYNNPQDYSDLMETGLLPPLDQGLPDQATDDAERPLPPPLDLHNLNLGPRTAHPPNLRNSTSPDDLPAQEDTAQGDLLRRLIETPDTMASVISALIQRGGIPQPEAAGNMGTDPGRHIYGKVIREHVRGQIRRILRQPNLAAYTRTSGPNGERYTNAPFPLIRAHILAQPLVWRQRHLPSGLADNDMDAIENLDRFLRTMVKHERTTLRNLLLIQVRPDPRRRAPGPIPRLFDLLVLIEQGLSGPRNALSRQDLASWATRPVRLRFAILRLLAVNHYLNRPPGETASQWEIIDRHLEMLAALSPVVLQAHIELIIRKDAHYFDGNKYIGDIPRETFHLPTDEEVNAEVARLNRTPAAERATQLADMTDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUU4</accession>
    <name>E3JUU4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_01150</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <subcellularLocation>
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            <topology evidence="1">Multi-pass membrane protein</topology>
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    </dbReference>
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    <dbReference type="GeneID" id="10543813"/>
    <dbReference type="KEGG" id="pgr:PGTG_01150"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01150"/>
    <dbReference type="eggNOG" id="KOG2533">
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    <dbReference type="HOGENOM" id="CLU_001265_0_1_1"/>
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    <dbReference type="OrthoDB" id="3639251at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:transmembrane transporter activity"/>
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    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter like domains"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
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    </dbReference>
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        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G00710)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-0813">Transport</keyword>
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        <location>
            <begin position="12"/>
            <end position="35"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="47"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="77"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="242"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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            <begin position="266"/>
            <end position="288"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="333"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP75819.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="417" mass="46074" checksum="A62B8A9EA4F84CB5" modified="2012-04-18" version="2">MMQNKSKQKVRKIGAGIQIPLIVTLWGLITCLQGLVTSYQGLLATRFFLGLVEGALGPATVLYMSTFYTRTELQFRIALFLSTICIAAAVSGLLTYEIIKLDGRWGHPGWAWIEGFVTCICGLTGFFILPSSIEKAKLLTDCEKNFLISRLQVAWKAAPSLTSPTTPIQKYPGKSTKAQICDAFKSPHVILMSIAQFASTSNIYSVAYFTPTVVTTFGYTPTATQLFSRLSYWSDRYQSRSLAAGFSATLAIIGFAIFYGSNSDKLRYASLFLSIPGVYGVMPSLAAWTADNSEPHVRKAAALACGGMVANIGGLFSIWIFTLGLPPRYALPTTINIFLAALIVVCCIVNVMWLRYAERNKVTKRDEILHKFTLVDSDEICDPEKDKMSKDPSDQVLISAQAWDHLGDKHPDYKYTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3JVI2</accession>
    <name>E3JVI2_PUCGT</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_02498</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="GeneID" id="10533297"/>
    <dbReference type="KEGG" id="pgr:PGTG_02498"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02498"/>
    <dbReference type="HOGENOM" id="CLU_012972_0_1_1"/>
    <dbReference type="InParanoid" id="E3JVI2"/>
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    <dbReference type="OrthoDB" id="2507581at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP76057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="209" mass="22802" checksum="96F2DF46F00CF420" modified="2011-01-11" version="1">MADAGAGSSDGKMVKIKPQDKSLGFDGNNVDRFLSDYQLAARLDGASDSDMAQQVRFFVRGEEVKDVLETLDGFETKECRCPHQLVGGDSNIVLLLVFDGGAREDLGSAYQRQDNDHDKAVDEVMKGQPALTFEDSRSSNPVSTSLFKQSNEIMNKMETDRRPKEAPVSAKAPATIDEISKMLQSFEQRLEKKFLASAAPPSSSVPPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXG5</accession>
    <name>E3JXG5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_02201</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
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            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321159.1">
        <property type="nucleotide sequence ID" value="XM_003321111.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXG5"/>
    <dbReference type="GeneID" id="10528761"/>
    <dbReference type="KEGG" id="pgr:PGTG_02201"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02201"/>
    <dbReference type="HOGENOM" id="CLU_1897246_0_0_1"/>
    <dbReference type="InParanoid" id="E3JXG5"/>
    <dbReference type="OrthoDB" id="10334260at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP76740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="134" mass="14770" checksum="5253F2CD848547CA" modified="2011-01-11" version="1">MKNGGTLPDSRLPTNQLLTILSYSLFVIPFPVLNQRPLLDPTLWKDATLKIAKSCNKMLQTNEPTAQLPSAFAAALAELKADNEAREARAVNRRNEAATQMVTMKRELTSLSRIGEGRAHITQGPSRIDEERAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZ53</accession>
    <name>E3JZ53_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03284</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77328.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003321699.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZ53"/>
    <dbReference type="GeneID" id="10537874"/>
    <dbReference type="KEGG" id="pgr:PGTG_03284"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03284"/>
    <dbReference type="HOGENOM" id="CLU_465499_0_0_1"/>
    <dbReference type="InParanoid" id="E3JZ53"/>
    <dbReference type="OrthoDB" id="2498748at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
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    <proteinExistence type="predicted"/>
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        <location>
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            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77328.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="476" mass="52348" checksum="710E3992EF4AC883" modified="2012-04-18" version="2">MQAHLSTPDSNPRRRLSSAISKLSLNKMKAFVSSSTIAPNLSSPGTDQSENASDSSLSVSPPPCPILHANSQTMKNYASQRSNKTPFREPALFPTSLNFSQQTIYLFESNEGEDDENLNFYLNDLLLGPSEPATSDQGHKDQTPEKTTKQRSFHLSGRHIKSKKFSLNAFPLTKSVSFQNHGKGLIKRSESTLFRAAKPSALTASPQTASHPVAEPEVLPKKEDLPRKISDPITDYSKFSERTRLLSLPTKLDTAQIKQTMPKEKLPTLLEAIPEQSNVSSPTFSTDSHTALLTPQRFENEAICPRIDMILTSSPFFNNNNSTLSPVLNQEPGTPILHSRNTNGCLFQTLTPYTFAAPETPTTCVNSISAHQHQATPHGKFAMSTGSPGIFNLPTPCSQPLRTPVRRQYSLLSPHYLPSPLPPRDSSSHGQASRGPGADMLATLEFLSDLCQKSPEYLEEQDSLHERASIQDSNEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    <gene>
        <name evidence="2" type="ORF">PGTG_03579</name>
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        <lineage>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
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    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP77623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="GeneID" id="10538831"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03579"/>
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    <dbReference type="InParanoid" id="E3JZZ8"/>
    <dbReference type="OrthoDB" id="10305207at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
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            <end position="110"/>
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        <location>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="116" mass="12842" checksum="C004EB63E03F977B" modified="2011-01-11" version="1">MLGGLLTKQFILIVPKDVLKALLGEPIYDKTDLDGHGHMAVPPYVPPEAACALKNEPSFLLPSPDYDAKSSLYLSVGYEKRLRLSCLTGSHPKKMPHHSRSSTSRARKSIELCRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3K014</accession>
    <name>E3K014_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
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            <taxon>Puccinia</taxon>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77639.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03595"/>
    <dbReference type="HOGENOM" id="CLU_2159658_0_0_1"/>
    <dbReference type="InParanoid" id="E3K014"/>
    <dbReference type="OrthoDB" id="10315110at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77639.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="117" mass="13315" checksum="BA7E80A17094D47A" modified="2012-04-18" version="2">MKQLDVYISASQPSKSISQLLNSTIESEFNMWTKTEMYRSVKDERPKPDDEDDDFDDPPPLPKPTAKMDDHIMSYPSKAANKLNQSMEASSKQLIDHLISTGHQTTMTCFFQPVNSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3K0A7</accession>
    <name>E3K0A7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
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    <gene>
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        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
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                <person name="DeCaprio D."/>
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                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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    </reference>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="75"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="252" mass="27285" checksum="99F8C9AD636C3E21" modified="2011-01-11" version="1">MCDVYLIVPPSPPLQQPKTASRPATPAANTRPDSPTVFPPPSGSDLDIINDRALTPTETTNYSEGHLNSMVSAEPSSKTSSNNPQSSQLTMTDLNFNNLNNLDIIPTQGGVMTTMNPLTTASTDSTSNIIDNPCLPSAPLPIDPTLPLPSVINNLDPVCHQTQLIIPPAQPDNVEVAAVGGIEILDNDNSNSTDARLAPEYSQATLDYYNDIQEYLQQTNADETEMKKKKKKKKKKKANPTSTPKNPILFYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3K1J3</accession>
    <name>E3K1J3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="7">50S ribosomal protein L19e</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_04124</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL19 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP78168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322587.1">
        <property type="nucleotide sequence ID" value="XM_003322539.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K1J3"/>
    <dbReference type="FunCoup" id="E3K1J3">
        <property type="interactions" value="459"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K1J3"/>
    <dbReference type="GeneID" id="10541798"/>
    <dbReference type="KEGG" id="pgr:PGTG_04124"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04124"/>
    <dbReference type="eggNOG" id="KOG1696">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_083919_0_1_1"/>
    <dbReference type="InParanoid" id="E3K1J3"/>
    <dbReference type="OMA" id="NRVWIDP"/>
    <dbReference type="OrthoDB" id="5407653at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01417">
        <property type="entry name" value="Ribosomal_L19e_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.240:FF:000001">
        <property type="entry name" value="Ribosomal protein L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1650.10:FF:000001">
        <property type="entry name" value="Ribosomal protein L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01475">
        <property type="entry name" value="Ribosomal_eL19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035970">
        <property type="entry name" value="60S_ribosomal_eL19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039547">
        <property type="entry name" value="Ribosomal_eL19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000196">
        <property type="entry name" value="Ribosomal_eL19_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015972">
        <property type="entry name" value="Ribosomal_eL19_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033935">
        <property type="entry name" value="Ribosomal_eL19_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057259">
        <property type="entry name" value="Ribosomal_L19e"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057260">
        <property type="entry name" value="Ribosomal_L19e_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006343">
        <property type="entry name" value="PRK08570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01280">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25476">
        <property type="entry name" value="Ribosomal_L19e_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01416">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48140">
        <property type="entry name" value="Ribosomal protein L19 (L19e)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL19" evidence="6">
        <location>
            <begin position="4"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="68"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="143"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="68"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP78168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="196" mass="22790" checksum="F711EFCCA998D879" modified="2011-01-11" version="1">MSSNLRSQKRLAASVLGVGKNKIQFDPTQLAEIGNANSRQTIRKLYKDGFILKRSITIHSRARTRAMHAAKAKGRHTGPGKRKGTAEARMPTKVLWMRRQRVLRRLLRKYREAGKIDRHLYHALYMKAKGNVFKNKRVLMDYIHKAKAEKTREKDLAEQMEARRVKNKAARERRAIRVEEKRAAIVAVEHQTEEKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2G4</accession>
    <name>E3K2G4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_04489</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322952.1">
        <property type="nucleotide sequence ID" value="XM_003322904.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2G4"/>
    <dbReference type="STRING" id="418459.E3K2G4"/>
    <dbReference type="GeneID" id="10536297"/>
    <dbReference type="KEGG" id="pgr:PGTG_04489"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04489"/>
    <dbReference type="HOGENOM" id="CLU_806842_0_0_1"/>
    <dbReference type="InParanoid" id="E3K2G4"/>
    <dbReference type="OMA" id="CRDEDAT"/>
    <dbReference type="OrthoDB" id="2507044at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16448">
        <property type="entry name" value="RING-H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="12"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="132"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="210"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="210"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="265"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP78533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="335" mass="37108" checksum="9E532883AD5270A8" modified="2011-01-11" version="1">MATTEVVVMPTCTICRDDDATDLNFGITACGHAFHSQCMEDWNRNQALRRRMTVCPCCNTNLSFTRSPTIRIHQLAKQRVVIIPDGHADPLEELKAATKKLEKLEADQAKLAVENNALKAKVTKLTYESKKRMVEESRNTPQGSYKVLNLDGHHGPDLEKERQIRKRQLHALHMGEQKRLLRQLEDSKEAYENLLAGFEPVGLTINTSAADRATPSHQRQPIDPVDSDRSSSVSIGVKRPRPSRTEKEKAKTPMVIEIDSESDNPSPPPGPPNSPAFTLNPFNPIPGPSVPKPFRALQASTSASKRRYDQASATPKINYTTRSFAKLASGSGPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3K344</accession>
    <name>E3K344_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04857</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78901.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323320.2">
        <property type="nucleotide sequence ID" value="XM_003323272.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K344"/>
    <dbReference type="GeneID" id="10537007"/>
    <dbReference type="KEGG" id="pgr:PGTG_04857"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04857"/>
    <dbReference type="HOGENOM" id="CLU_2293045_0_0_1"/>
    <dbReference type="InParanoid" id="E3K344"/>
    <dbReference type="OrthoDB" id="10274463at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172359" evidence="1">
        <location>
            <begin position="20"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78901.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="101" mass="10937" checksum="E9569496F536F9E2" modified="2012-04-18" version="2">MHLITTTIVILSAFGAAFGRLECTIARPHGMCGTPTPGGLRLIMSNLTPTYEADPLVYAGCQTTPYCGDDWMWKRVDAAMGSGEGIPKDATFDQHCQEMPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3K373</accession>
    <name>E3K373_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04886</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323349.1">
        <property type="nucleotide sequence ID" value="XM_003323301.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K373"/>
    <dbReference type="GeneID" id="10537118"/>
    <dbReference type="KEGG" id="pgr:PGTG_04886"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04886"/>
    <dbReference type="HOGENOM" id="CLU_1016113_0_0_1"/>
    <dbReference type="InParanoid" id="E3K373"/>
    <dbReference type="OMA" id="IICGVEV"/>
    <dbReference type="OrthoDB" id="2498045at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172979" description="Secreted protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="278" mass="30199" checksum="A7285DCF676D1BDA" modified="2011-01-11" version="1">MVNFFSFVFVASALSMAAADSKISVITEETLLGPQTLTAITSPLLEKRDPSPELDKRGFLGNLLELPTSIISEVLPVATRTVPEPINVRASARVSFKQIKVHAYLLDTLNAILPHTHAIRRLCTGTVNPDADELSWQLVTELRAIFEILNGCLYKVKTCGKAPTPSGIPGGRTPTLDDICQLFFKIMCEIRECCKLIGSLCIKYKTVRRVCQDTLRQICGCLSNIAVRCGVEVGDMSSGLGSRFATVPNFFTGIQFGFSAFPSILGNANASFSFNASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3K4J3</accession>
    <name>E3K4J3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_05741</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79420.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323839.2">
        <property type="nucleotide sequence ID" value="XM_003323791.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K4J3"/>
    <dbReference type="GeneID" id="10531244"/>
    <dbReference type="KEGG" id="pgr:PGTG_05741"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05741"/>
    <dbReference type="HOGENOM" id="CLU_1816734_0_0_1"/>
    <dbReference type="InParanoid" id="E3K4J3"/>
    <dbReference type="OrthoDB" id="5418029at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP79420.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="142" mass="15471" checksum="3F4C3A6234E30B3F" modified="2012-04-18" version="2">MLLNYGHNISTPLTSQFTIPPPEPHIPQSFTKAVFRAIEKIKVDTCSLSAATNGAKVAAWMQAILVEGSNLFNGTVTSNLDGFIKGTDDSEGRYEFVYMVLKLCVIVVLEGKSDGTTSNNYAQMMTELQGSTRQARTPPPKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3K513</accession>
    <name>E3K513_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_05649</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79328.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323747.2">
        <property type="nucleotide sequence ID" value="XM_003323699.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K513"/>
    <dbReference type="GeneID" id="10531152"/>
    <dbReference type="KEGG" id="pgr:PGTG_05649"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05649"/>
    <dbReference type="eggNOG" id="KOG2341">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018835_0_0_1"/>
    <dbReference type="InParanoid" id="E3K513"/>
    <dbReference type="OrthoDB" id="10304049at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP79328.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="455" mass="52906" checksum="9275098782B5DD68" modified="2012-04-18" version="2">MLDFLRLPMDLAEKETFREQMKTVIKSLSQLFEQKIYELRGQEFTWEPLPSDVSLMIGEKWKVAWLSSVADISKTWKNLDPKSRRAGSIIRRGSDLMIDSFIDMDRLGIISPGCLSHFLNKVDRGNLIATHVISRYSFKFPNIYDVYLNLNTKLSLTECPSTKKMTGLMRELEPGSWERIEFYCLRNWLIQYQSIYPKEGRFAAIKDRFLEVASPDNEFPVTKLQAFLGALFTHISSQSRIRENEKAKKLTEFKFLYDMMSFIMSYRSSSISAEFMGGMQRSWLLDQIRAFEESVGLISSIMYSVHVRRKATLLTIPTRRPVLRKMLAKMTDKLVDSSSNRRISSFSDFADGDLSSGSYEQQAKNNAWDLESKTKPPALIQALSTDPIEIRDSIEKINHQFRNYRIQLEALKQRHAFIHPTFCAFVGDFSPLSVYLLRVYELEVERTILHNTRHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3K5V5</accession>
    <name>E3K5V5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05847</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79526.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323945.2">
        <property type="nucleotide sequence ID" value="XM_003323897.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K5V5"/>
    <dbReference type="GeneID" id="10531354"/>
    <dbReference type="KEGG" id="pgr:PGTG_05847"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05847"/>
    <dbReference type="HOGENOM" id="CLU_158808_0_0_1"/>
    <dbReference type="InParanoid" id="E3K5V5"/>
    <dbReference type="OrthoDB" id="10540981at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79526.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="115" mass="13295" checksum="EE9D08A8D3C91843" modified="2012-04-18" version="2">MIDPCLRIKVHNQVDPERQPKDLNEAFTSNTPAEVTNSNPDENTKEKVGQPETARLREADAPESSRSRGTARPGTARPREAIRIPRREVFLVEEERDFRFSTLIHGLYLLYWYSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3K7I7</accession>
    <name>E3K7I7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nitrogen regulatory protein areA GATA-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_06434</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80478.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324897.2">
        <property type="nucleotide sequence ID" value="XM_003324849.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K7I7"/>
    <dbReference type="FunCoup" id="E3K7I7">
        <property type="interactions" value="294"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K7I7"/>
    <dbReference type="GeneID" id="10539489"/>
    <dbReference type="KEGG" id="pgr:PGTG_06434"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06434"/>
    <dbReference type="HOGENOM" id="CLU_834542_0_0_1"/>
    <dbReference type="InParanoid" id="E3K7I7"/>
    <dbReference type="OrthoDB" id="515401at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031930">
        <property type="term" value="P:mitochondria-nucleus signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006808">
        <property type="term" value="P:regulation of nitrogen utilization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013860">
        <property type="entry name" value="AreA_GATA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053043">
        <property type="entry name" value="Ras-cAMP_regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28014">
        <property type="entry name" value="NEGATIVE REGULATOR OF RAS-CAMP PATHWAY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28014:SF1">
        <property type="entry name" value="NEGATIVE REGULATOR OF RAS-CAMP PATHWAY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08550">
        <property type="entry name" value="GATA_AreA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitrogen regulatory protein areA GATA-like" evidence="2">
        <location>
            <begin position="16"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="148"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="242"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="269"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP80478.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="333" mass="36900" checksum="98F0F86B3DB5C8E7" modified="2012-04-18" version="2">MNLTINLKDELPIGTLWLAFSRSKPIIQDGHRLENLSWRLWQRELKQNNSQNSQPTHQLHHLTRPTTAHNTFRSTPHQLSPHPLAQSSLIITTHISPSASLHSTNNWLLSPPATENNNNNSLPSDSRSSSSYFTLQQPSRPSIDQHYSSGSNTSSSLATSPSIQAPPDNHLLQDHNKHIKQTVDRPKHTAHNLHPLPRRATSSTATFKHKRRPPVPTRLHSHQSTTRSVSPRLVQPYYCRRSSLPSSSPEQVQEPPKPLDPSHPTQLITSPSSVTHPHSSQNHPPQPSLAHDPDSQITLSAGYNSSGSLPSLVSPIIPETPNNSQVSAPLPTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>E3K9F1</accession>
    <name>E3K9F1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Histone-lysine N-methyltransferase SET5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">SET domain-containing protein 5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_07323</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-lysyl-[histone] + S-adenosyl-L-methionine = N(6)-methyl-L-lysyl-[histone] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10024"/>
            <dbReference type="Rhea" id="RHEA-COMP:9845"/>
            <dbReference type="Rhea" id="RHEA-COMP:9846"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61929"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:10025"/>
        </physiologicalReaction>
    </comment>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP81071.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325490.2">
        <property type="nucleotide sequence ID" value="XM_003325442.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K9F1"/>
    <dbReference type="STRING" id="418459.E3K9F1"/>
    <dbReference type="GeneID" id="10536393"/>
    <dbReference type="KEGG" id="pgr:PGTG_07323"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07323"/>
    <dbReference type="eggNOG" id="KOG2084">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031650_0_0_1"/>
    <dbReference type="InParanoid" id="E3K9F1"/>
    <dbReference type="OrthoDB" id="438641at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042799">
        <property type="term" value="F:histone H4K20 methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045814">
        <property type="term" value="P:negative regulation of gene expression, epigenetic"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd20071">
        <property type="entry name" value="SET_SMYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.270.10">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001214">
        <property type="entry name" value="SET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046341">
        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46402:SF2">
        <property type="entry name" value="HISTONE-LYSINE N-TRIMETHYLTRANSFERASE SMYD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46402">
        <property type="entry name" value="SET AND MYND DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00856">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00317">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82199">
        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SET" evidence="8">
        <location>
            <begin position="179"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="105"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="356"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="650"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="112"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="125"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="399"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="412"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="652"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="677"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00042380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP81071.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="691" mass="77754" checksum="424A5923D5F6A769" modified="2012-04-18" version="2">MTINGHSTQQENNNHEIQPKQLQELDLNDQQKPNNGNSAEEEDKPTATSQQGIVPHDGQLLDAVLPIRAKTPSLGVPKILAKIKETNPDWQLSEKRLRKFLNSNLFPHSNERNNSNPSNTNRLSDPSSAHQLRSPSEPMWIPNYAWEKDWEAASVDHSPNEPISSLDDTLLLECRQRSVKIEPVRFPASNKGSGLIAKDHLAKGTQVMKEDAFVWVPPTKNMSRSVMSGQSCAFCGRAFSMQRTKAQPSCPHHPAPGSKEAIAAQQLMVTTSTSLTTGGQSVTEKPAGPEPCYMRYCTRVCAQRSVDYFGPLVCPQRNPEYMQLLRFTDAEDFRGAHMVFKMFARMLIANESGELAENPSFKPEPSKPLPNGGSIHNGESESQEPNGSDDPVSAGGNNPKKHKKPKSKKKSLANQIPPSTGPSVKFEETYSRIQGLATMSDRARREKYHGGGHLGSHFADVRYGQMWRNGWHAVIKALHINTGYRPQAADPNLQVPSTDSFWDSFWKRRLDPDTIAHYFGLKSFLEFIGKTSLNREADHGLYLLHSRFNHSCDPNAVVLKHPGSEDIHKSAPSTIYVMARKDIAKDEEITLSYINPDLSLDARRRKLFEDYLFSCFCSRCRKELEQEEERKHYQELMDKMRPTCNEELYHPNLTSNNNNNSLKHRIGQQPATTTAETADDEDDEDMSFSCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3K9M2</accession>
    <name>E3K9M2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_07370</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP81118.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325537.2">
        <property type="nucleotide sequence ID" value="XM_003325489.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K9M2"/>
    <dbReference type="GeneID" id="10536440"/>
    <dbReference type="KEGG" id="pgr:PGTG_07370"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07370"/>
    <dbReference type="HOGENOM" id="CLU_2639271_0_0_1"/>
    <dbReference type="InParanoid" id="E3K9M2"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP81118.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="77" mass="8613" checksum="C8F2DAA89886CC59" modified="2012-04-18" version="2">MVLKTICTYPRHGTGRGPYIANTTQEIVRAYRRRNGVDLSQFDGSARMGPTFGAMLLCKLSFIHLYILQSVMDLSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBH1</accession>
    <name>E3KBH1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Golgi to ER traffic-protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07726</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81477.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325896.2">
        <property type="nucleotide sequence ID" value="XM_003325848.2"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KBH1"/>
    <dbReference type="GeneID" id="10537223"/>
    <dbReference type="KEGG" id="pgr:PGTG_07726"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07726"/>
    <dbReference type="HOGENOM" id="CLU_002667_0_0_1"/>
    <dbReference type="InParanoid" id="E3KBH1"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053094">
        <property type="entry name" value="Zinc_metalloprotease_ZmpB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193:SF2">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="418"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="515"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="681"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="778"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1257"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="541"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="551"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="687"/>
            <end position="698"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="743"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1273"/>
            <end position="1306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1324"/>
            <end position="1340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1342"/>
            <end position="1351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1352"/>
            <end position="1366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81477.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1385" mass="150857" checksum="D4568D38381941CF" modified="2012-04-18" version="2">MRDQASGLGASRNGSKSKGPAGPAKSGPRGSEWVTVPPKKVPQEMQRYLSAQRPPIGGQKSKRSSPTPEDFRTIGREGAQLDPALSDDENGSEEELHVNETLTSQPPETPKSLEVKKKKKPILTTGPKASGSKSSPKVVVPKTTLGPPASTGGSVNVGHGLNPKLLADALARQEGASKDTGSGSEKLPPIVGHRANSKMIADALARHEQATRGIKSPDNMIPVGNGEFMSLAECRAFLDQFPKLSPVGVGTPARALSTGSSSDARKTTLLSTPLVAPTAKNSSLVIQDPPNTGGPPKVPKPVLASHAPGVVTPLHKGMMTRTPSTSSSITNAMLPNLATASLDQLKSMRRICSNTREMLSTSVTEIRSEDLHLLAPGAYGFQDRHLRLMTRYCSQLDQKIAEAENAGISDAIEVIDLTTDEPPDNGPSTSSVTGSAKRLSRPSEASESDDSPVMAKKTRTTVPPPPPGQYIQPNLPPYSFRDIPDKDLLAGIAPKFPVNRQPSISEFTSQFQSDLSLPYSPTGPPGGLLASSSKPAAPGGNVSNQVGQAVTDTQQPTTQDPFKQRLPKSAPLSTPSHANLPTAPRTRSGGVGKAAQVPGTRPDQLVNKNVSKPSMSDLATPVDMGDPVWNASDTEGRGLNTDTGADADGGDMVVDPEALKWVSGKGKGKGLDPVLNVSDKDARALNTPGNTKQSTAHKQTAADPEGAEVDHTGAVADVEDRVDEPEPPKKDRRKGKGKGQATAKEKAAKTTKKKLADMTAEEKAARLEERKEKAKARKEEKAHCVHAIPPQPGNLLAPMLTPKECEEARRAHKRMLTDSRNPQWDFGPELMDKVADMILAFKKDRREDSFYTKSPQFTPEPHMIDAIKEVFEGDFPELIMKCPRLLQVDAADPFWTKEAVDLSIIKKAHDSKDSVKAASWITLYGMMLRVDHYPDYTEDDNTVKLFDAAFRKIHTLTVSSFNEFHRYVSPSDKPVDKSIPTFKQDTALQAGRFVINKIKSLKVGAATSAHHGNARTGNGLMLLQRRIWDTLLCVLMMQQSLFQQDLEHCIQTGTYPNQTMLVSQHMSANQRAQSLYKHGDVKDTKNSKAMKEWGEDRLAAFGSMAIFFLYGAAGWWQFLTDSHHYNQKDVWVLVHLAKGKSDWIYERGHLSKRRTEDTPWYRIDSFVRWLLVKTKMHVRITDKVDWEAAPKFWAEHVTSHNIARLALQDVLSEVCLVTPLKSLNFNGEPAPDVVLEEDLQASVEKCRAKLTAGWQGLVEGTAGGESSDGSSLTEEEEVEEEEEEVEEEEEEDEDDEMDEEESEEEGEIRAPRPSGKPVHGKIPSSSSSDSGTEDSSSHSGPGDEENDDEDTDPHRSDSDGGVEERQRRRREHPLILYTSKVQKSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBK4</accession>
    <name>E3KBK4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07749</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81500.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325919.2">
        <property type="nucleotide sequence ID" value="XM_003325871.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KBK4"/>
    <dbReference type="GeneID" id="10537238"/>
    <dbReference type="KEGG" id="pgr:PGTG_07749"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07749"/>
    <dbReference type="HOGENOM" id="CLU_040957_1_0_1"/>
    <dbReference type="InParanoid" id="E3KBK4"/>
    <dbReference type="OrthoDB" id="2506684at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81500.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="323" mass="35652" checksum="2382B83524C045F4" modified="2012-04-18" version="2">MSEKNSRPPNHPVSAKHLAYVTEGSSANLPSGQQYGIFTAPGFISCNPADPTDYEVILSANTSAPFSLEAGYVYSLTGKLMVMGPDSPPIFTYFPETMIRVCKTDHFIGDVTNKTSVDAIGTAISVDRETTDNNGVVENNLMVTMLHNDWDPQARCVRAFNVRYKIPGRRNMANTHVLFQPNREFFLIGFLCDWDMDKEMPVVEVLALSWINPPNAARVQVTGNRTTPNNSPSRPGRNVVHFSQRTQSRERRDPSAPILDQGIGSSTGCLSLVEDIASQNETNHDPVETEGNNQSVELPSQSSPRKRGKISLSEPAKRKKDDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCJ4</accession>
    <name>E3KCJ4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">ATP-dependent RNA helicase SUB2</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">ATP-dependent RNA helicase sub2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PGTG_08191</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">ATP-binding RNA helicase involved in transcription elongation and required for the export of mRNA out of the nucleus. SUB2 also plays a role in pre-mRNA splicing and spliceosome assembly. May be involved in rDNA and telomeric silencing, and maintenance of genome integrity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the DEAD box helicase family. DECD subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EFP81942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326361.1">
        <property type="nucleotide sequence ID" value="XM_003326313.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCJ4"/>
    <dbReference type="FunCoup" id="E3KCJ4">
        <property type="interactions" value="823"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KCJ4"/>
    <dbReference type="GeneID" id="10534142"/>
    <dbReference type="KEGG" id="pgr:PGTG_08191"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08191"/>
    <dbReference type="eggNOG" id="KOG0329">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_1_0_1"/>
    <dbReference type="InParanoid" id="E3KCJ4"/>
    <dbReference type="OMA" id="YAHVEPK"/>
    <dbReference type="OrthoDB" id="10265785at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17950">
        <property type="entry name" value="DEADc_DDX39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000111">
        <property type="entry name" value="DEAD-box ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000168">
        <property type="entry name" value="DEAD-box ATP-dependent RNA helicase 56-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 21" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="10" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="20">
        <location>
            <begin position="50"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="18">
        <location>
            <begin position="81"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="19">
        <location>
            <begin position="284"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="17">
        <location>
            <begin position="50"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00037698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00038213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00040177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="EFP81942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="433" mass="48237" checksum="C4DE12B1DF15BD32" modified="2011-01-11" version="1">MAEHAEDLIDYDEDDAGVAPSAAVTSTTNGAAAPLNGKDQKGSYVGIHSTGFRDFLLKPELLRAISDLGFEHPSEVQQECIPQAILGMDVLCQAKSGMGKTAVFVTATLQQMDPKEGEVSMLVLCHTRELAYQIKNEYARFSKYMPDIRTAVVYGGIPIKQDAEMLKAKDKCPHILVGTPGRLNALVRDGLLKCNTVRTFVLDECDKMLESVDMRRDVQQVFLATPHAKQVMMFSATLSKEIRPVCKKFMQNPLEIYVDDEAKLTLHGLQQHFVKIDEAAKNRKLNDLLDTLEFNQVCIFVKSVSRAVQLDQLLRDCNFPSIAIHSGLGQEERIDRYKQFKAFEKRILVATDIFGRGIDVERVNIVINYDTPGEADSYLHRVGRAGRFGTKGLAITFVSSEADQTVLDAIQSRFEVAITELPDHIEASSYMNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCY1</accession>
    <name>E3KCY1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_07656</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82259.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326678.2">
        <property type="nucleotide sequence ID" value="XM_003326630.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCY1"/>
    <dbReference type="STRING" id="418459.E3KCY1"/>
    <dbReference type="GeneID" id="10529632"/>
    <dbReference type="KEGG" id="pgr:PGTG_07656"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07656"/>
    <dbReference type="eggNOG" id="ENOG502SR2M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058091_0_0_1"/>
    <dbReference type="InParanoid" id="E3KCY1"/>
    <dbReference type="OrthoDB" id="1600564at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050592">
        <property type="entry name" value="GDSL_lipolytic_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642">
        <property type="entry name" value="GDSL ESTERASE/LIPASE EXL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45642:SF139">
        <property type="entry name" value="SGNH HYDROLASE-TYPE ESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173442" description="SGNH hydrolase-type esterase domain-containing protein" evidence="2">
        <location>
            <begin position="29"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP82259.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="364" mass="40992" checksum="17779C0ABB765970" modified="2012-04-18" version="2">MWISKHRTCNRFIRFVVSPLLVITKASCLVDSPSDPSELQESFQPSWTNYSAFIVFGDSYSDNGHPRSPEDQPSLAAKPAVGGRFCDGPVWDEYLAQNLSNSADSNITFLNYAYNGAHIRNKLSNQSPSPVPDTAAQIQTYLTDLEKYVQHPISGKVFEGRILHALWIGINPILSIWRSASSGNQTLNADTLLTPELAETLNTQVEEFSKQLLLLSNNPSLSKFQTDYMVMTIPPLTNTALALSQSSQASKGDVTLAQQYIELLAQMTDHFNTKLVEAVQKMTRQFSWHKRRITVFDTVDFWDDVRSAPQRFGIQSLGSCYTDPNKPPCKKPDLYMYWDILHPSSIFHANLSNAIMQFLDELNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3KD71</accession>
    <name>E3KD71_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_07501</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82104.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326523.2">
        <property type="nucleotide sequence ID" value="XM_003326475.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KD71"/>
    <dbReference type="FunCoup" id="E3KD71">
        <property type="interactions" value="71"/>
    </dbReference>
    <dbReference type="GeneID" id="10534635"/>
    <dbReference type="KEGG" id="pgr:PGTG_07501"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07501"/>
    <dbReference type="eggNOG" id="KOG2533">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_0_1_1"/>
    <dbReference type="InParanoid" id="E3KD71"/>
    <dbReference type="OrthoDB" id="2985014at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000013">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000018">
        <property type="entry name" value="MFS transporter permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF85">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G00710)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="125"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="150"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="185"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="218"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="330"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="386"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="417"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="59"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="14"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP82104.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="454" mass="49712" checksum="CC2A0FA638623271" modified="2012-04-18" version="2">MEPVDKKHPDDPQSDPTPVSSGSGLSSDVNQVLLREEDLSPNRSKKDEKRAILKLDLIVLPLIVMFYFFSSLDRSNIGNARVAGLQTALKLSDHQYSVALTSTFITYVLVKVPANLVLKRIGAHIFLPMISCAWGLVTFCQGFTTNFGGLVVARLFMGLAEGGLYPAILLYLSHSYSRDQLQMRIAIFYSSASLSGSFSGLLAYAILHLHETWGKPAWAWLFFIEGAATMLFATMAAFIFPASINTNRLLNDKEKQILTARLARKAPSTLDPNEKFKFSEVLEAIKSPHVIILTITQFMSGTAAYSVTYFTPTIVHSLGYSPSRTQLMTSPPFAVAFVVMLAGGYFSDRCHARALTAGVSAAVAFVGFMLFLLLDPSQTHAKYGSLFLAIPGVFSLIPPIGAWAANNSDGHYRRATAIAFSYSTSTVGGILSTWLFPHSQAPRYRPGIIVNLVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KEI7</accession>
    <name>E3KEI7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326973.1">
        <property type="nucleotide sequence ID" value="XM_003326925.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KEI7"/>
    <dbReference type="GeneID" id="10544520"/>
    <dbReference type="KEGG" id="pgr:PGTG_08750"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08750"/>
    <dbReference type="HOGENOM" id="CLU_1262061_0_0_1"/>
    <dbReference type="InParanoid" id="E3KEI7"/>
    <dbReference type="OrthoDB" id="2501672at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="143"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="219" mass="23956" checksum="7B0D4297F45FDECE" modified="2011-01-11" version="1">MMITVPDDIMIECSHQDRVASTKDCWQPGSSLQLWRADHRAINTYWIPLLLHQPTPTTLSHTNKQSKTILTSTNKPQHPKTNMNTNTNQRSGGRVSDDMRDSSMNPTKDMMSGMDSGMDDNMMNRDRQGEDQGLMGKMSSMVGGNKNDNNQSSKMMGGVAGGRDSNRGQQEASMGDKVKGTFEQAKGKVMNDPHTARKGDMRKDPAGAGSNDFMGGAGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KEM7</accession>
    <name>E3KEM7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_08937</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82741.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327160.2">
        <property type="nucleotide sequence ID" value="XM_003327112.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KEM7"/>
    <dbReference type="GeneID" id="10530124"/>
    <dbReference type="KEGG" id="pgr:PGTG_08937"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08937"/>
    <dbReference type="HOGENOM" id="CLU_562761_0_0_1"/>
    <dbReference type="InParanoid" id="E3KEM7"/>
    <dbReference type="OrthoDB" id="2679012at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34226">
        <property type="entry name" value="PROTEIN CBR-ABU-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34226:SF1">
        <property type="entry name" value="PROTEIN CBR-ABU-10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP82741.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="485" mass="52760" checksum="8D5AC072299ED517" modified="2012-04-18" version="2">MSVLNSLVPRSGLFVIVTWSATEILAPVVKDFNSLLWEQPIVEGSDSHFSSPDHELVPALASNTFITVAPLELDKQYKPMHDQLSLPSDPLLNEPAPLALTSSPSLTLAHMLLGSQIGEIAHNHQYIQNCNLPSIPHLVELENLAPIPPPSSTLVDVLMGSEKCGMENDHQYAHGYVPPVTLLAEPVNLAPTSLSSSKLGEVLLESETGGLAYNHQYAQRNLQPFPSHAQDPSTIGHAQDYSIDGHSEYPLIVRHAQYSSIIGHVPGPSIFGHPQNTWIAGHSPDPSIFGHALAPSILEYAQDYPIFGHAQDLSTVGHAQDPSIFEHFQDALDVEHTQDSGIIHYPLVVGDVQDPSIVVPAQDHFIAAHAQDSSIMGQPHYASITGGAPDTSILGHAQHPSNVGHAQLHTSTVGNFQDPSNIEDFQDPSIIGHKPSKAIKENLIENPTDPNFQKIMKFFDLLQSSLAYQQRIADDDPKVVIQIYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>E3KFD6</accession>
    <name>E3KFD6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Tricalbin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGTG_09931</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178284">
        <property type="protein sequence ID" value="EFP82963.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327382.2">
        <property type="nucleotide sequence ID" value="XM_003327334.2"/>
    </dbReference>
    <dbReference type="FunCoup" id="E3KFD6">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KFD6"/>
    <dbReference type="GeneID" id="10544674"/>
    <dbReference type="KEGG" id="pgr:PGTG_09931"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09931"/>
    <dbReference type="HOGENOM" id="CLU_001661_0_0_1"/>
    <dbReference type="InParanoid" id="E3KFD6"/>
    <dbReference type="OrthoDB" id="1029639at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090158">
        <property type="term" value="P:endoplasmic reticulum membrane organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061817">
        <property type="term" value="P:endoplasmic reticulum-plasma membrane tethering"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035621">
        <property type="term" value="P:ER to Golgi ceramide transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00030">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04044">
        <property type="entry name" value="C2A_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04052">
        <property type="entry name" value="C2B_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04045">
        <property type="entry name" value="C2C_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04040">
        <property type="entry name" value="C2D_Tricalbin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21678">
        <property type="entry name" value="SMP_TCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037761">
        <property type="entry name" value="C2A_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037765">
        <property type="entry name" value="C2B_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037762">
        <property type="entry name" value="C2C_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037756">
        <property type="entry name" value="C2D_Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056910">
        <property type="entry name" value="TCB1-3_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017147">
        <property type="entry name" value="Tricalbin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052455">
        <property type="entry name" value="Tricalbin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46980">
        <property type="entry name" value="TRICALBIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46980:SF2">
        <property type="entry name" value="TRICALBIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24920">
        <property type="entry name" value="C2_TCB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25669">
        <property type="entry name" value="SMP_MUG190-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037232">
        <property type="entry name" value="Tricalbin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="9" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="192"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="SMP-LTD" evidence="14">
        <location>
            <begin position="234"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="425"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="573"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="714"/>
            <end position="833"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="1117"/>
            <end position="1235"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="13">
        <location>
            <begin position="1370"/>
            <end position="1489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="12"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="874"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="967"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1544"/>
            <end position="1622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="33"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="886"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1569"/>
            <end position="1580"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EFP82963.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="17">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1622" mass="178700" checksum="DF1801EECD95C6D0" modified="2012-04-18" version="2">MSVKQFDYAINHQQQKKSSLDTQPQTSTILQSDPDHLHHPNHSDSLKSITTKLDNTRSQSTLDPIKTDIDKPSAITQEITSAILTDGTNPEELPLLDHTAGQDNLNQLLESIPGQLNESPLTNKIPDWYKIGWAAQIEHVDLSSKSDQDEAKHLDLIETFLSEVYYGTWFHNAAVIFFAVAASHYITLFGGGWASLIIILAICATYYTTSIRRVRKNVRGDVSRELAKQRLFQDHETVDWLNNFLNRFWLIYEPVLSATIVASVDQILVASTPSFLESIRMSTFTLGSKAPRIDFIRSHPETENDVVVMDWKFDFTPNDVSDLTAKAAAGKINPKIVLTIRFGKGVIGAAKDIVVENISFTGTIRIRIKLMNNFPHLQLIDLSFLEKPEFDFILKPIGFDLNMIPGLSGFIESQVHASLGPMMYDPNVFTLNLEQMLAGALVDSAVGLLQIAIASAQGLKAVKIGGGTPDPYVTFSIGARLNLDRTKVKHSTQSPNWKSVHFLLIHSLNEILTMEIMDYNEVRKDTSLGTASVDLQTLVTEPEQEGLMVPIMYQGKPRGEIRLSMVYHPCLVPKQLENGETEPVPETSAGVTRLVLHQAKELDYKRTGTSNLSPYAKIYLNGKEILKTPVIKRTNNPVYEAFTEVLISSKPDAVFTINMFDDRHGDDPKIGSVNVKLPDLLELTSGEQKLDWFPLVGAKSGKLRISAIWKGIMMAGAINGASAYTPPIGILRFHFDRAEDLKNVEALTGGKSDPYVRVMRSGIVLSRSQIHNNDLDPVFDEIIYVPVHSLKDSFRLEVMDYQHLTKDRSLGHIDLETSKLVEINKEEGTNGYKSTGKHQYSEYLKQEGKKALVKGKMNFTVEFHACKPIKFNRFDTPPSDVDKMKTGQQSDKLSMLTSSDSQSITLINPEGSGLVNGNSVNGKESLEMLDGRPASKSTQAGYDASSIDGSPRAKRLDAVTLSHQHAKPSIDSFGSHSRHLRDEKNSGPPLVELSKDELLTHQSGILVFNIIGGVIAKKNARLEFLFDDGYWPSYSTEPSKSTHPKWDETGESFIRELDWSRCILKLNVADKDTREEVYAEYTSDLKDFLNDCLDGPHDFVLQDLEGGNRSTISIQCRYIPVPITLEPRESINNMGLLTVLLDHGKDLMAADRNGYSDPYAQFVLNGAKVFKSSVQKKTLNPKWTERFDVEIPSRASAEFYVHVYDWDRVGASDKLGQARIDLSNLEPMVQTTVIANLSLSDNKQKGQVQFRMTFRPHFISRSRQATSNFTGGFGRVATGLGGTVLSAGAGVGLGAAKGVGTVGGGVIKGVGGVGKLGYAGIRRATGMGNKRASVVEETVPRVPVLDSDLSLPPTASANDSRKRIQSNDLAAGQATHSTPPKANLLTDDFKLNIVVMGLVGMENPGGKMYVQVKRNGKAVHDTKVIRASPTTGLEWNEICTLDAKLGVESELELVVCEKRKLGKDREMGSVRLKLWETVHPDQGRFEAEIDQALEESGGDPSAGSANSAAGHLRFKLSIIDASSSTNVGKEIYNHTRRSISAGNHTFGNLFGSHSNHHHHHQSKAQIEDQQASSVAASSAQNELFLNHDPNPSPAKPPSVFGGRSQRSVSGAGASRFSLHRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHN5</accession>
    <name>E3KHN5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-directed RNA polymerase III subunit RPC9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_09523</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic RPC9 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328229.1">
        <property type="nucleotide sequence ID" value="XM_003328181.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHN5"/>
    <dbReference type="STRING" id="418459.E3KHN5"/>
    <dbReference type="GeneID" id="10532497"/>
    <dbReference type="KEGG" id="pgr:PGTG_09523"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09523"/>
    <dbReference type="eggNOG" id="KOG4168">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_839733_0_0_1"/>
    <dbReference type="InParanoid" id="E3KHN5"/>
    <dbReference type="OMA" id="HNLRTIE"/>
    <dbReference type="OrthoDB" id="1746530at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005574">
        <property type="entry name" value="Rpb4/RPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038324">
        <property type="entry name" value="Rpb4/RPC9_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038846">
        <property type="entry name" value="RPC9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15561">
        <property type="entry name" value="CALCITONIN GENE-RELATED PEPTIDE-RECEPTOR COMPONENT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15561:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03874">
        <property type="entry name" value="RNA_pol_Rpb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="269"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="293"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP83810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="331" mass="36886" checksum="F83A8EA73DAEB786" modified="2011-01-11" version="1">MVAGGVSSEFLIASAGIANYSRSIGNVHNNDETGVTVIPVVGFYALKARAPCLKSGGRLKTRHGHIADPTEICDHDAAQVMQKPLGWGVRVVFSRPVEEIPLEEFGNVKRPGRMEILNPNLIPLSAFETLELVREIKTKQELEDQRAIELNANQQPQQLPPQPNNNLDQLVKNILTHFEHPNHHLPQAKQSTQSILSLCESLRNRFGQVSGRANGLTKAERLQICDLAPTELVEIYLIIEECDSRFTEEEIQEIIDLVKQNLSTDRRTTHTLNHKPVNSLQNGDTENDRDGFDYEEDPNFNLGDIDDDDALVAEAREGGPPADQELDEVPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHN8</accession>
    <name>E3KHN8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09526</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83813.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328232.2">
        <property type="nucleotide sequence ID" value="XM_003328184.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHN8"/>
    <dbReference type="GeneID" id="10532500"/>
    <dbReference type="KEGG" id="pgr:PGTG_09526"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09526"/>
    <dbReference type="HOGENOM" id="CLU_041322_0_0_1"/>
    <dbReference type="InParanoid" id="E3KHN8"/>
    <dbReference type="OrthoDB" id="10276072at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="221"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83813.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="416" mass="45547" checksum="08057E30B60CE788" modified="2012-04-18" version="2">MTSPHPPHSYYSISSDYSPTQAFDEDGDSFTSFGSIETPPRLRAMIRSIFDGTLNSPIRSFPDSDYGDASSPLERVTDHLSAAIVEEGMNEGETFGDYRARLLASIPPLPLDGAAMVYSSNQDGEYELDDSVVAPTPHTDQEHYTAVVGSPAPASLGYVPDATNEVGQDVGLLDPNDNGVFPVDPRLQLPSEDDDRSTIDSVFSLLDPYEDPTDTTYVPPKKSKQSRVGRKRSSGSLLAKPKAAFVRPRDSRGRFVSLQSRNPGKLKANDNDDQPLPSSSSSSFLHRAVADSNHPYLAETARNTFLNTVLTSPEEAFPFTFHLPDPFAAHSSLAGPSVLGTHPFIPPTPFINIRPDDPRLQFRTAFVDLLRVIVPFLSNFNLAPDDHDNFKLLSHAVVAAQDCPDVADHQLQFDQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIC5</accession>
    <name>E3KIC5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09763</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EFP84050.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328469.2">
        <property type="nucleotide sequence ID" value="XM_003328421.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIC5"/>
    <dbReference type="GeneID" id="10545146"/>
    <dbReference type="KEGG" id="pgr:PGTG_09763"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09763"/>
    <dbReference type="HOGENOM" id="CLU_1278197_0_0_1"/>
    <dbReference type="InParanoid" id="E3KIC5"/>
    <dbReference type="OrthoDB" id="2499848at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="65"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84050.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="248" mass="28290" checksum="436B0706C13FF12B" modified="2012-04-18" version="2">MKLPLGTLPDHDPFPLRSRTRPLHKLPRYMAKRPLLSIKIPSLIDRVGKDERTPEVSWFSPFTPDSPSSRSQSPSMSNELVKTADAQRDTADEDAQQSRSNVMEEDEENRNTQPTISRLGSRWSRRFPHGCLQTMFQQVQRTPTRHKHLSTISVSPYSAIDLQRPRRSSPHHTLMNDHDGLSNSRRTSNPFDYLSLRRHSKIDSPTRHDKTPTQLGIRPVLPVASPAEMLTSIHTPKHLNAPWSSTTC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIP4</accession>
    <name>E3KIP4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10547</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328588.1">
        <property type="nucleotide sequence ID" value="XM_003328540.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIP4"/>
    <dbReference type="STRING" id="418459.E3KIP4"/>
    <dbReference type="GeneID" id="10545347"/>
    <dbReference type="KEGG" id="pgr:PGTG_10547"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10547"/>
    <dbReference type="eggNOG" id="KOG1223">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1705134_0_0_1"/>
    <dbReference type="InParanoid" id="E3KIP4"/>
    <dbReference type="OrthoDB" id="10324058at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="entry name" value="Anthranilate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="ADC synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="154" mass="17370" checksum="457FAAF0A3A49AB2" modified="2011-01-11" version="1">MAVLPSFRWRNRICILQLCLPLRAHHPPSLVGPDWYGRVGVHDLRYRLVVSNVIIPSASSATEDQVGAAYERAKVKIIDVCNQLESSHVPLPVQQDPFPSKKDRTPPVSNVGKQGYDSFVTSLKQNICKGDITQAVPSQRLKKETRLHLFNVYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIZ3</accession>
    <name>E3KIZ3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_10646</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328687.1">
        <property type="nucleotide sequence ID" value="XM_003328639.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIZ3"/>
    <dbReference type="GeneID" id="10545531"/>
    <dbReference type="KEGG" id="pgr:PGTG_10646"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10646"/>
    <dbReference type="HOGENOM" id="CLU_900580_0_0_1"/>
    <dbReference type="InParanoid" id="E3KIZ3"/>
    <dbReference type="OrthoDB" id="2510239at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172030" evidence="2">
        <location>
            <begin position="21"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP84268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="309" mass="34127" checksum="C67FFD7A1AEE47C4" modified="2011-01-11" version="1">MRQPICCFLFLHTLAHPFFASISARPTFPGVYDPPFLASGAARFPGDSLDSCGTLDSMWKRPRVDNPGTSFPHGDSPLLPEQDDRNLCYEREHGNHDSGLLECVVGPSFSGRPSKLPKLTTDHPLENMGQVWQAMPSEQDTETQEEHESIWEWDAAQIEGLLDNLFDDGVNSIHHFWRDPHQETLDSLATSSRYDQVTPSSVLSYQTGGHALDWDSPRIVHSPLGSHPVHQKAVGSSNNERGEPIASVFPKYGNEMGVVPGMPIDPPGLGYPSTKDQHNIPFLYSWSEPITTVHPLASLGSLGKVCRNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJ72</accession>
    <name>E3KJ72_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178290">
        <property type="protein sequence ID" value="EFP84347.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328766.2">
        <property type="nucleotide sequence ID" value="XM_003328718.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJ72"/>
    <dbReference type="GeneID" id="10545694"/>
    <dbReference type="KEGG" id="pgr:PGTG_10067"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10067"/>
    <dbReference type="HOGENOM" id="CLU_046892_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJ72"/>
    <dbReference type="OrthoDB" id="2501728at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173935" evidence="1">
        <location>
            <begin position="19"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84347.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="371" mass="41718" checksum="5F38392591D8EA8C" modified="2012-04-18" version="2">MKVLFITSLLMLDSVTSGMDSPGPGGLLPDLNIPLADTSLDPPCSPITINSMQLPPLLETSNAENNLAEPLPISDSSRKVISTSDAPSSSSKRKWESNLLSSEFITVLDTSAPPTKRKENLNSSSLRKEKIRSLAVIMEETRNALPGPQRQLKLSDKGYYRPGSFKMRDQNLTESPGNTSAIKHLFEPGELPSGVLPEFFNVYDWRYVRACSEGEKSEVSEEGSLGWFLEVLNQCKYSETMSPDRFFWIPRAQAVSILKRYQKLNLDFGNQFIILDRAQTLRDITLRVSDDKLDLDKYPVLAKIQNELEAGLKLQMRSDPDDVTLGRIQSIIAHIKKLTKITTFLMITYLSLFKEHPNDYLSAELNSNRKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJ80</accession>
    <name>E3KJ80_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_10075</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178290">
        <property type="protein sequence ID" value="EFP84355.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328774.2">
        <property type="nucleotide sequence ID" value="XM_003328726.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJ80"/>
    <dbReference type="GeneID" id="10545782"/>
    <dbReference type="KEGG" id="pgr:PGTG_10075"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10075"/>
    <dbReference type="HOGENOM" id="CLU_034307_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJ80"/>
    <dbReference type="OrthoDB" id="2499263at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP84355.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="409" mass="48016" checksum="F616E580AE0EEE98" modified="2012-04-18" version="2">MKIIKSKIERLEKLFKEELVPLVRKKDEETFWRLFEHVTFEHDPLDHESGRVISRLKESLQSLYIDPPTTRSAQEFPETQFHFVQKSLQALNLLNEKELIPHAPSVITSFFNSNRVVKVVGKYLVQVFGPEGYPDFQRLVSFKPDFQFLKTDLTTSSMHWWLNYDVEEDGIRLYKWEGFLQESYIKRFHWKGLSSRPCVEARRSLISDLKDIITQALVTNDPREFLASFWMVRFVMTYSVMDDLSKILAAWSKDFMIQYRLTEAVLEMHEFLNRSYRRQSSFSSEAQIFSSLQHEKHWANFSSLVPTDPQDLKKFKQIQARCGKLLETLDANNQSKDRVGINSLLKSTLNLDSQLLLNGWLQPVNVQLTITKSTKSSVDSFDKRLSNTKPSFRRKIINDIFKPEKKKAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJV2</accession>
    <name>E3KJV2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_10736</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328996.1">
        <property type="nucleotide sequence ID" value="XM_003328948.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJV2"/>
    <dbReference type="GeneID" id="10546166"/>
    <dbReference type="KEGG" id="pgr:PGTG_10736"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10736"/>
    <dbReference type="HOGENOM" id="CLU_1587328_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJV2"/>
    <dbReference type="OrthoDB" id="10422254at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP84577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="168" mass="19094" checksum="0F2EE6A772F6FA27" modified="2011-01-11" version="1">MWPSSRVMVEEICGQMSFPEHVTGDRDWPGLRSNTSCEAFPSPGDFPSLFPSAIFDHPDRPYHLASQHPLARPKTAARRSYFQVPHMLWSSLYHLLCDWVKVRVFQSPQELLSWPVHRSDSINSQGVCSCKHSSRAHHQKSLSSLTGQPNESGRDGSFWDRLASFISD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KK69</accession>
    <name>E3KK69_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_10853</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84694.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329113.2">
        <property type="nucleotide sequence ID" value="XM_003329065.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KK69"/>
    <dbReference type="STRING" id="418459.E3KK69"/>
    <dbReference type="GeneID" id="10526661"/>
    <dbReference type="KEGG" id="pgr:PGTG_10853"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10853"/>
    <dbReference type="HOGENOM" id="CLU_011407_4_1_1"/>
    <dbReference type="InParanoid" id="E3KK69"/>
    <dbReference type="OrthoDB" id="3364670at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040521">
        <property type="entry name" value="KDZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18758">
        <property type="entry name" value="KDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP84694.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="492" mass="57743" checksum="244D95A25A0DE2F7" modified="2012-04-18" version="2">MTPEQANRCTESHKAADDKRNESTWKGCDDTGLMGCCCRHDAAIYLTNIYKSGEQRHFPMTIIKKLLSDIEPNRQVGILYDIGCTMDKYIDRRQLIPQYRSQLKFGTSVFHAYAHNWLCQLEFHPRFNKGWGLSDGEGLERLWSYLSSLVSPLRYATRNHRLAAISHRVKHHNNRGIKQLPYWLRRKFHQAIKRRQETQSTLTTLLNKRNPHERLGQNFTIDFFKKQWKAQQQFHTEHTEAEIDRRNKLVALYKREATVEYMRYAYNQSHNHHNCLTLHLRERLQGPEIFLATVAEVRELMDSIVAQSDSLASEIAELTGRMEGGSNRDYEEEKLRLLLWDAKSELFVQAVHIHAERQPLLNSHIMGSHLGTQGKENIIKALQNRRPAVKKIIDTFNGLYEQFQQKYPEQHLSDAHDHPLTYDDFVEWEMDHPFWNDGLYHHMEAPWSIDPDVKTGINCILILRRVQEEFELIAQELARAVGWAIRNPLTTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>E3KKH6</accession>
    <name>E3KKH6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 11">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="7 11">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_10272</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 10 11">Belongs to the peptidase C12 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="7 11"/>
    <dbReference type="EMBL" id="DS178292">
        <property type="protein sequence ID" value="EFP84801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329220.1">
        <property type="nucleotide sequence ID" value="XM_003329172.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KKH6"/>
    <dbReference type="FunCoup" id="E3KKH6">
        <property type="interactions" value="795"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KKH6"/>
    <dbReference type="GeneID" id="10526775"/>
    <dbReference type="KEGG" id="pgr:PGTG_10272"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10272"/>
    <dbReference type="eggNOG" id="KOG2778">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018316_0_1_1"/>
    <dbReference type="InParanoid" id="E3KKH6"/>
    <dbReference type="OMA" id="XAKEKQN"/>
    <dbReference type="OrthoDB" id="1924260at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09617">
        <property type="entry name" value="Peptidase_C12_UCH37_BAP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.532.10:FF:000009">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.532.10">
        <property type="entry name" value="Peptidase C12, ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001578">
        <property type="entry name" value="Peptidase_C12_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036959">
        <property type="entry name" value="Peptidase_C12_UCH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017390">
        <property type="entry name" value="Ubiquitinyl_hydrolase_UCH37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041507">
        <property type="entry name" value="UCH_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10589:SF16">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE ISOZYME L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01088">
        <property type="entry name" value="Peptidase_C12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18031">
        <property type="entry name" value="UCH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038120">
        <property type="entry name" value="Ubiquitinyl_hydrolase_UCH37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00707">
        <property type="entry name" value="UBCTHYDRLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52048">
        <property type="entry name" value="UCH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52049">
        <property type="entry name" value="ULD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7 10" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 10" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4 7 10" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UCH catalytic" evidence="12">
        <location>
            <begin position="4"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8 10">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="8 10">
        <location>
            <position position="166"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="10">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="site" description="Important for enzyme activity" evidence="9 10">
        <location>
            <position position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR038120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR038120-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR038120-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU361215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS52048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP84801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="320" mass="36154" checksum="37432EEDD2E5E8DC" modified="2011-01-11" version="1">MSEGWCLTESDPAIFTILLSELGVSGLQVEELWSLDNQSLEQLKPVHALIFLFKYVNSDQRDPSKQAGEQQPAPPGTWFAHQVITNACATLAILNAVMNIPSTSSKIQLGQELSQLKEFSVDLDSQMTGELLTNSERIRAVHNSFARPDPFQRDEPKQMSTEDAYHFITYLPLQGKLYELDGLNGLPVFHGKIPEGQSWTSLAREVIQNRINTYSSTEVHFNLMAVCEDRLNVLEERKASMTVDSMGYENLKMEIEAEEAKRKRWAEEVAIKRHNHIGLIHALMVGLAKHGSLAERVNLAKQKMVDRQRNRTGGHEMQLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3KKZ0</accession>
    <name>E3KKZ0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_10436</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178292">
        <property type="protein sequence ID" value="EFP84965.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329384.2">
        <property type="nucleotide sequence ID" value="XM_003329336.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KKZ0"/>
    <dbReference type="GeneID" id="10526940"/>
    <dbReference type="KEGG" id="pgr:PGTG_10436"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10436"/>
    <dbReference type="eggNOG" id="KOG0017">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054434_0_1_1"/>
    <dbReference type="InParanoid" id="E3KKZ0"/>
    <dbReference type="OrthoDB" id="2500181at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="283"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="290"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="329"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP84965.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="343" mass="38264" checksum="F57F605B42B0DC75" modified="2012-04-18" version="2">MIPCRSSSTYFQSDSQIVYRRFKLGYTNFQFLSLTDHLVSASILDLLDMPYRLTQNPSPLIPSVPQLDGNSVVFPAWRSRIEDVLAIQGVLDIVQGKVPRPKAEATDAKPLVRTEKGYNPEEFRTDWDTLSDVARSTIKLTLSVDLSIRYRDIKPASKLYDQICEAYEKNTRARRLMLEDAFWTAKHDPNVPIAKWIAPVRMAASDLTSVKLTPHNQQVCDRLLRGLDDSWKPIRDHLVYSPNEVLLDGTIGALESHEVSTQVSTDHYDASASMTKAKPKLGCWNCGQKGHHSSKCPNPSIKKKSGTQSSSTEARAGAVSFATLGNYSEGEEDDDDGCDVVWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLC3</accession>
    <name>E3KLC3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_11267</name>
        <name evidence="4" type="ORF">PGTG_17114</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP85098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="DS178344">
        <property type="protein sequence ID" value="EFP91257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329517.1">
        <property type="nucleotide sequence ID" value="XM_003329469.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335676.1">
        <property type="nucleotide sequence ID" value="XM_003335628.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLC3"/>
    <dbReference type="STRING" id="418459.E3KLC3"/>
    <dbReference type="GeneID" id="10527079"/>
    <dbReference type="GeneID" id="10529003"/>
    <dbReference type="KEGG" id="pgr:PGTG_11267"/>
    <dbReference type="KEGG" id="pgr:PGTG_17114"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11267"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17114"/>
    <dbReference type="HOGENOM" id="CLU_055324_1_1_1"/>
    <dbReference type="InParanoid" id="E3KLC3"/>
    <dbReference type="OMA" id="CCARNNI"/>
    <dbReference type="OrthoDB" id="76487at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023:SF4">
        <property type="entry name" value="GLYCINE-RICH PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="2">
        <location>
            <begin position="108"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP85098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP91257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="335" mass="37788" checksum="7CEEDF9949F78826" modified="2011-01-11" version="1">MPTRSANWLPTEDQQLAKSWLKISEDPIISTGQKRAEFFKRVAEDYNDYSGGIERDGTMAMHRWGHIQKACLKFSGIYHKLKNNRPSGSVLSDLLPDTKKAFYEQEGKQFIFELAWMVVKDSSKFNSLAQTRGDQSKQATTPAPTPTPSTPAAQSDAALGNSQPEDIAQIGSNNSWNRPAGIHATKRAMKQEHFNAKKIKVMSERSSNYRERTIAMKKTNEIRQQVAKAEVNQVNMEIMSRKEDELPDDVSREFLRLQKGEILEDLREQIRERQKLAAAKEKQTEASTNEALAASSERDSTFEDDPSSNAEDSQYIGDEDEDDIADLDPLLDTLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3KM13</accession>
    <name>E3KM13_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_11531</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178294">
        <property type="protein sequence ID" value="EFP85362.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329781.2">
        <property type="nucleotide sequence ID" value="XM_003329733.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KM13"/>
    <dbReference type="GeneID" id="10543905"/>
    <dbReference type="KEGG" id="pgr:PGTG_11531"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11531"/>
    <dbReference type="eggNOG" id="ENOG502RZ5C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037534_0_0_1"/>
    <dbReference type="InParanoid" id="E3KM13"/>
    <dbReference type="OrthoDB" id="4138492at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010905">
        <property type="entry name" value="Glyco_hydro_88"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41814:SF1">
        <property type="entry name" value="CELLULASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41814">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07470">
        <property type="entry name" value="Glyco_hydro_88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173720" evidence="2">
        <location>
            <begin position="19"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP85362.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="447" mass="49204" checksum="2D9ECAA21726E16F" modified="2012-04-18" version="2">MLKLALVQVSMLISSALSVPSSSEYSGIQPRSANSVLSERAAPNPPQVHTGTVYSFEKTATLVYEVLNRVANHSWEWGAQAEVIFERNYPEYSVYSNTRQLPLSSQDGPLKPPSGLIKFIEPIIAKRVPGTKPIVDGDGASGDPASLGVAVLVSAATSNKERSEYFQKLADDQLDWLLNHVPINENGAISQRDKELQYWSDYVYMAPPFLAYYGAATCNATLLEIAYNQAKQYRTILQDGKIKVWEHIVSGSFEDRAFWSTGNGWAAAGMMRLYGTFMNLREPKLRELTSPWREDLANWVAEIVKGAYAYQYKDSYLLPNYLANTNASHNYAECSGTALIAAAAYRLASLRPSLAADLPLDAINAARVAIFTKYTNSKTGAVAPVVDPLNWNADKAFNGTKPDVGFVSPEGQAFTILLFEAWKAYVASLDCKANPSHPHCSTGYQRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNK8</accession>
    <name>E3KNK8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11639</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178297">
        <property type="protein sequence ID" value="EFP85883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330302.1">
        <property type="nucleotide sequence ID" value="XM_003330254.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNK8"/>
    <dbReference type="GeneID" id="10544465"/>
    <dbReference type="KEGG" id="pgr:PGTG_11639"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11639"/>
    <dbReference type="HOGENOM" id="CLU_044507_0_0_1"/>
    <dbReference type="InParanoid" id="E3KNK8"/>
    <dbReference type="OrthoDB" id="2509616at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="483" mass="53695" checksum="57FDDECB7D3B9C3A" modified="2011-01-11" version="1">MSSPHDMDTSEEDERPAGDGDASTSQGGRGGAANADDDEFDRMLGGGGEDHEDDEDSDSGIEDPTAALKHQVEREKRKRAHENSQANTRKVRKAVVGQQLPSQSGGLSRSIRNFIKFMMGMPASLSAYPAPPTKKEWDTWDNWTKNRYDKVTAHLKSFTEKNKEVAKPTLKKMYRDEMDRIRKHLTPPSFKPSGDVLNSAINIPIHVKKSCEQEIQLAGFGRLTFEWTARSFTASPWNGTLGTILIKHYYQWAKSQAGTLWDETSNMEKILDRWVQGQGKLMKKGDRSDGKSLDDLKREKARKASISRGKTKIAEKRRDVAMKKMGNQPSLALLFTKETISDWEDQPAPAKAKRIALQWRSAAFTMCAIKLDEMALTICKTTAEKAATRKLLERDPVSIQGVAVEFDMVPRKLPLDAYNPTFLLGLSPIEKEHLDAKEAIDLDAKVQTLKEMTQRGGTGMAQMARQKFGDPGLGGLPGPSSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPH9</accession>
    <name>E3KPH9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Enoyl-CoA hydratase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_12170</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178299">
        <property type="protein sequence ID" value="EFP86214.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330633.2">
        <property type="nucleotide sequence ID" value="XM_003330585.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KPH9"/>
    <dbReference type="STRING" id="418459.E3KPH9"/>
    <dbReference type="GeneID" id="10540224"/>
    <dbReference type="KEGG" id="pgr:PGTG_12170"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12170"/>
    <dbReference type="eggNOG" id="KOG1680">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_6_1"/>
    <dbReference type="InParanoid" id="E3KPH9"/>
    <dbReference type="OrthoDB" id="2139957at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000104">
        <property type="entry name" value="Related to enoyl-CoA hydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF158">
        <property type="entry name" value="ENOYL-COA HYDRATASE (AFU_ORTHOLOGUE AFUA_2G10650)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="293"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="295"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP86214.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="315" mass="35164" checksum="036607B2E645EF51" modified="2012-04-18" version="2">MKSKPTSPIPAHGPTVQCTFLEEGRIMMVAMNRPKYLNSMTDEMQSELERVLDYAEDEPSIWAIVITGTVTPNVTKAFCGGQDLKDWFKKKDEDQRERLLKTPKGFGSISRRHSRKPMIAAVDGLCLGGGLELLLNCDLVIATEESTFGFPEVSKGVLVAQGGIPRLLHHCGRSLASELLFLGRSIDAITARDRFRIVNEVVPTSEDLMPAVLKMSKQIISNSPAAVQLTKLALVDTLRRGHRSFLRKGELIKLEELESEIGNGIEQSTVSTILRDEFEDWFAGPDMQEGLQSFVEKRKPKWTDPVKKKPRSSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQ62</accession>
    <name>E3KQ62_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_12393</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178300">
        <property type="protein sequence ID" value="EFP86437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330856.1">
        <property type="nucleotide sequence ID" value="XM_003330808.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQ62"/>
    <dbReference type="STRING" id="418459.E3KQ62"/>
    <dbReference type="GeneID" id="10540452"/>
    <dbReference type="KEGG" id="pgr:PGTG_12393"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12393"/>
    <dbReference type="HOGENOM" id="CLU_134573_0_0_1"/>
    <dbReference type="InParanoid" id="E3KQ62"/>
    <dbReference type="OMA" id="GASAPCH"/>
    <dbReference type="OrthoDB" id="2505052at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040521">
        <property type="entry name" value="KDZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18758">
        <property type="entry name" value="KDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP86437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="169" mass="19883" checksum="01141E7BD129A70C" modified="2011-01-11" version="1">MHRVSQLMSVTENVGMLYNIGCSMDKYINLRNLLVKDRPQIKFGTSVFHTYVHNWMCQLDYHPHFNKGWGLLDGEGLEFGQWAAQRAFQADHTDVEDHRMKKMANIYEQENVIDLLSFFINQHLALRNWLRNPRVSLASEVEVRELLDLIESRETAFARCLEHRLTFGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>E3KRA0</accession>
    <name>E3KRA0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="7">C4-hydroxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_13207</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
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                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
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                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
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    </evidence>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
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                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
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                <person name="Cantarel B.L."/>
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                <person name="Coutinho P.M."/>
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                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
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                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
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                <person name="Pearson M."/>
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                <person name="Tuskan G.A."/>
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                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
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    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87015.1"/>
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    </evidence>
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        </source>
    </evidence>
    <sequence length="56" mass="6393" checksum="2D1DC68707AAD8FB" modified="2011-01-11" version="1">MVNLHQSIVVFEQPIMITNLDRILGPSDARILSSIIIQIFLLIQLRFLLLSFSLPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KRW5</accession>
    <name>E3KRW5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_13259</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EFP87040.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331459.2">
        <property type="nucleotide sequence ID" value="XM_003331411.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KRW5"/>
    <dbReference type="GeneID" id="10542121"/>
    <dbReference type="KEGG" id="pgr:PGTG_13259"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13259"/>
    <dbReference type="HOGENOM" id="CLU_033090_0_0_1"/>
    <dbReference type="InParanoid" id="E3KRW5"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP87040.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="239" mass="26766" checksum="737BDF2F7CBF0F70" modified="2012-04-18" version="2">MEEALVDQLQSSLLPLLQGQINTLFQALDPAGLRNQPRPKLSLVLQTQAELDDSLDQIKYLTATLCPDPATQPHRTDDHGLERFKSCRLYRLKANVELVLPWRMCEIFEAADKLIQKMELSSAPSIPGSPEIESLDKSLHVAVLGALDTIKKMANCLQASELVIAQDLWKSSRLAIENQLQSIIENLNLSMINRLNLEQALKEKFLGQSVIQLAKLTLPIFKLSKIFFSKVSKRGLALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>E3KSZ3</accession>
    <name>E3KSZ3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Trehalose-phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_13607</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">In the C-terminal section; belongs to the trehalose phosphatase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the glycosyltransferase 20 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178306">
        <property type="protein sequence ID" value="EFP87379.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331798.2">
        <property type="nucleotide sequence ID" value="XM_003331750.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KSZ3"/>
    <dbReference type="FunCoup" id="E3KSZ3">
        <property type="interactions" value="54"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KSZ3"/>
    <dbReference type="GeneID" id="10537947"/>
    <dbReference type="KEGG" id="pgr:PGTG_13607"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13607"/>
    <dbReference type="eggNOG" id="KOG1050">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002351_3_0_1"/>
    <dbReference type="InParanoid" id="E3KSZ3"/>
    <dbReference type="OrthoDB" id="755951at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005946">
        <property type="term" value="C:alpha,alpha-trehalose-phosphate synthase complex (UDP-forming)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004805">
        <property type="term" value="F:trehalose-phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03788">
        <property type="entry name" value="GT20_TPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01627">
        <property type="entry name" value="HAD_TPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000036">
        <property type="entry name" value="Alpha,alpha-trehalose-phosphate synthase subunit Tps2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1020:FF:000002">
        <property type="entry name" value="Trehalose-6-phosphate synthase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1020">
        <property type="entry name" value="Trehalose-6-phosphate phosphatase related protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001830">
        <property type="entry name" value="Glyco_trans_20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006379">
        <property type="entry name" value="HAD-SF_hydro_IIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003337">
        <property type="entry name" value="Trehalose_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01484">
        <property type="entry name" value="HAD-SF-IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00685">
        <property type="entry name" value="T6PP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788">
        <property type="entry name" value="TREHALOSE-6-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10788:SF123">
        <property type="entry name" value="TREHALOSE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00982">
        <property type="entry name" value="Glyco_transf_20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02358">
        <property type="entry name" value="Trehalose_PPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="80"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="136"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="169"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="294"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="983"/>
            <end position="1014"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="80"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="139"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="169"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="185"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="983"/>
            <end position="992"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP87379.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1146" mass="127344" checksum="F23142ADA9D27C95" modified="2012-04-18" version="2">MYDRAQVHYGLAQERPDEDDWEPPQLDGLPELHNIRHRISLIEEEIRSKGKLKLTGHVIMACHALPYLCNLHLDPTTTTDTTNTNTIPEQQQQQQQQQQQASVKPDQQLPPSPTTPLTASHNSTFRQSLEIRPGDLLTHPQSLHSPTCTPIPSSGILSASKTSSLLDTSIPAPTSVTNPTLNPVSSVASHRNNNNNNTHNHNHSSDNLQPSTLTADEEQEEPAFKWILEPRRGHAALNAGIKSLSNYRSITLCAYPADIRIANHSNELPEPIEASKLSELQKLSLDSGLRHLARSASPDQSPLNDHHQLPIDPNGPKRGIECVPVWVGDRSAGLHYEGFCKKYLWPIFHYMPLSEHQDKKEENLAWKAYEEVNYAFAKRIASVYQPGDLIWIQDYHLLLVPRMLRKMLPKCHVGLFLHSPFPSSEFFRCLPRREEILDGMLGADLLCFQTYSYGRHFISACIRVMGLESVGNGIECNGMITDITYCPIGIDADRIELEKQLPGVRPKMDALRKLYEGKKIIIGRDKLDPTKGVLHKLKAFEYLLKSYPQWRNRVVLIQVTSPSPTDSPQLATKISELVDHINATYGTLHFQPVHHYHQTIERDEYFALLSVADLAVITSIRDGMNTTSMEYTICQEENKNPLILSEFTGVTGSMKSAVKVNPWDLGDVARAIDQCLNMSEAEKVTRHAQLYSRVKSHSSSVWASTLLLQLLQCLRSDQTKESVKLLDEEDMVKKYKQANKRLLLFDYDGTLTPIVKNPKDAIPPPGLIDALERLCADERNIVYIISGRDGSFLQKHLGSIAGLGMSAEHGCFLREPGAKEWIDLTENIDMSWMDDVEEIFKYYEARTLGAFVEKKISSVTWHYRNADPQFGLFQAKECQALLESLQERMPIDILKGKKNIECRPAHTNKGEIVKRLLYTSPDVGFALCVGDDKTDEDMFRALKMLRSNASLDGHAMGHVHSTTIDPPQTLSLYPILARSSSIGPGQRISQDGSAAAAAAAGGGGGAGNNTKRPIRRRYSSNTSMHATYSCQNSPISAPSVPQFGLPSVISTASSSPATGGAIGSTSMSSQAMKASSTVGSSSLHHMLPSPKLEPVESVLEPESIFSLVIDENESRKTLAEYRLDRPLQLVAVLARLSQLSPPPSAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3KT24</accession>
    <name>E3KT24_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13638</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178306">
        <property type="protein sequence ID" value="EFP87410.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331829.2">
        <property type="nucleotide sequence ID" value="XM_003331781.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KT24"/>
    <dbReference type="GeneID" id="10538139"/>
    <dbReference type="KEGG" id="pgr:PGTG_13638"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13638"/>
    <dbReference type="HOGENOM" id="CLU_1078250_0_0_1"/>
    <dbReference type="InParanoid" id="E3KT24"/>
    <dbReference type="OrthoDB" id="10307965at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="159"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87410.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="258" mass="29364" checksum="A8E4FD8AB45EBBCE" modified="2012-04-18" version="2">MVLAEPIDHEQIMRANAQQIETDASTVKQLKANYEGQLNKWSFDANNDLRAFQMKIDLSDEAKTSKLQDIKDKFQDALRRISDLEKKAEVDESAQTRLQIQDANKIHKLSSLDPENIGKDLQLQQEELDGIKNVLDFFEKVEVLIHHLKSGVDQSLERTGSFDQRLRNLEEQLVQASEQKAAAGTTNNMKDLETAITALQRDISTLQTTGHQLAMEELKNVRDKLDLVEVRKLLKRFPGGKSINGFIFSKHNEILNQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>E3KTM1</accession>
    <name>E3KTM1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inner nuclear membrane protein enriched at telomere/subtelomere region</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13514</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178308">
        <property type="protein sequence ID" value="EFP87728.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332147.2">
        <property type="nucleotide sequence ID" value="XM_003332099.2"/>
    </dbReference>
    <dbReference type="GeneID" id="10539126"/>
    <dbReference type="KEGG" id="pgr:PGTG_13514"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13514"/>
    <dbReference type="HOGENOM" id="CLU_002733_0_0_1"/>
    <dbReference type="InParanoid" id="E3KTM1"/>
    <dbReference type="OrthoDB" id="2503928at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005637">
        <property type="term" value="C:nuclear inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071763">
        <property type="term" value="P:nuclear membrane organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044780">
        <property type="entry name" value="Heh2/Src1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47808">
        <property type="entry name" value="INNER NUCLEAR MEMBRANE PROTEIN HEH2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47808:SF2">
        <property type="entry name" value="LEM DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="430"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="670"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="811"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1037"/>
            <end position="1327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="671"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="698"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="811"/>
            <end position="844"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1045"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1074"/>
            <end position="1083"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1115"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1317"/>
            <end position="1327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87728.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1348" mass="140452" checksum="ED267E08C22793DE" modified="2012-04-18" version="2">MNIEEIISCSTNPEPPPDWMLPDFNPMKVKVQEIRGILLENSIRPASSNKKADHAALFRKHLLPRIPELLQTHCSVKASCAGIFDVRTGRYLKDEDTPIELRRRNTAIEAGSSEKAKNRSSAGLSNQTPERPSPRRTSRRVSDTTMLDGEAQSKMSPKTSSKSDYLHAQAGLLLHALTTFTGPSFQTPQRESTRRTAAPGNTESTPSTRKSPRFASPASTHQQPGSSKRGTSATVPISEVHLDRSVKKPAVSPKDVPSKQPPQPAVLPKKGASPCHPVLPRTSNPAKTKRVVSAPSTPAPDDDFGSELTELSSEDDEIRAVAEAVLASDSSEGEADVKPIRVPSPNTEQAATMNPNTKVRFDVPTPVGIPHKTAVSPRSSTNHQPHAQNPGIVDMRFPRNGKIIFSDVPADKSTYPTYLAPPGVPKPSIPNLIVAKPAPPSSSTTNPPTYLPPAGVSKPSTPSLVPEPAPPSPKTTDIRDPPTGPKHAESNSVLVETAVTSTVSVAQELSSLDPTHGWYSVPSHSTKLASEDNLLDHPPALPHIYQPLVPTPQVVEPLVDLATDWYPCPLDEIFDVAKGNDCSGTPTLIGQSQTMKSLATLELAFPPTGPTEATPPGASLASTSSIFPDKLEIASTPEHALAYKLCAARDTDTVLGTPTPQELSSAIALDQTATRSSSGHIEASQFESASANDVDMASPTSTSHETLTSIRPDEAPQFESASANDVDMASPTSTSHETLPSIPTDEAETSSAPGPFVAPDIAPTEATVPGVSSASAPCVFAELLEIASVFKPVLAAGLCAVRNANISPGTPTPQELSSAISVDQTASQSSSGHVEASQSDSASPDNIDMASPPSTWRETFSPIPACDVATSSSRDSSASPVLASASTGYDGCTLAAPPSDDNPWHEESELRSLPSLTWHEWPFVQKGNLSTLIPVALIEVAVPSASISADLVDSQPAAPSPPVMTPQVNSVVIDEAAVPAEFHSMDPGDSTLILPLPTSADEVEKEPVPEHVDAPQLVSSNDGDFLLALELDLADDVDLSPPSPSSISSGPLDTQPAEPVTAAEVESVPVVEAAESESADDADYSPPSPSSISSGLPDTQPAEPISAVEVESIPADEPAPQLLLALELDPAEDADFSPPSPSSISSGLLDTQPAEPVTAAEVESDSADDAAAERALAAESESADDGDFGPPSPSSISSSLLDTQPPEPVTAAEVESVSADKPAPQLLLALELDTADDADLSPPSPSSISSGPFDAQPADPITTVEGESIPADEPAPHVLLAFELDPTEDADLSPPSPSSISSGPLDTQPSGPLDTQPAEPVTAAEVESVPVVEAAASELADHADCSPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3KV60</accession>
    <name>E3KV60_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptide-methionine (R)-S-oxide reductase</fullName>
            <ecNumber evidence="5">1.8.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_14367</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-methionyl-[protein] + [thioredoxin]-disulfide + H2O = L-methionyl-(R)-S-oxide-[protein] + [thioredoxin]-dithiol</text>
            <dbReference type="Rhea" id="RHEA:24164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="Rhea" id="RHEA-COMP:12313"/>
            <dbReference type="Rhea" id="RHEA-COMP:12314"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16044"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:45764"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="EC" id="1.8.4.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the MsrB Met sulfoxide reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.4.12" evidence="5"/>
    <dbReference type="EMBL" id="DS178312">
        <property type="protein sequence ID" value="EFP88283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332702.1">
        <property type="nucleotide sequence ID" value="XM_003332654.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KV60"/>
    <dbReference type="FunCoup" id="E3KV60">
        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KV60"/>
    <dbReference type="GeneID" id="10535674"/>
    <dbReference type="KEGG" id="pgr:PGTG_14367"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14367"/>
    <dbReference type="eggNOG" id="KOG0856">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031040_8_1_1"/>
    <dbReference type="InParanoid" id="E3KV60"/>
    <dbReference type="OMA" id="DEQWRAE"/>
    <dbReference type="OrthoDB" id="44061at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033743">
        <property type="term" value="F:peptide-methionine (R)-S-oxide reductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.20">
        <property type="entry name" value="Peptide methionine sulfoxide reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028427">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002579">
        <property type="entry name" value="Met_Sox_Rdtase_MsrB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00357">
        <property type="entry name" value="peptide-methionine (R)-S-oxide reductase MsrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46081">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46081:SF8">
        <property type="entry name" value="PEPTIDE METHIONINE SULFOXIDE REDUCTASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01641">
        <property type="entry name" value="SelR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51790">
        <property type="entry name" value="MSRB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="MsrB" evidence="7">
        <location>
            <begin position="67"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="31"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="31"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU365044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP88283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="203" mass="23291" checksum="C49FF34EFF468DE5" modified="2011-01-11" version="1">MSLLKPTQPLLRLLLNRQWTPTNQRIRLRPQTQTSLSHQPQQSFSSHTPQNHQENRMTESSNLPKTEEEWRLRLDPEQFRILRKKGTEMAGTGEYNKHQPDHGIYECAGCAAPLYRYDHKFNSGCGWPAFFDAFPGAVSRHEDRSFGRVRTEITCTNCGGHLGHVFKGEGYGHPTDERHCVNSISIKFNESTPAAEDKLNPKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3KV80</accession>
    <name>E3KV80_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14259</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178312">
        <property type="protein sequence ID" value="EFP88175.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332594.2">
        <property type="nucleotide sequence ID" value="XM_003332546.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KV80"/>
    <dbReference type="GeneID" id="10535401"/>
    <dbReference type="KEGG" id="pgr:PGTG_14259"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14259"/>
    <dbReference type="HOGENOM" id="CLU_647475_0_0_1"/>
    <dbReference type="InParanoid" id="E3KV80"/>
    <dbReference type="OrthoDB" id="2498397at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905761">
        <property type="term" value="F:SCF ubiquitin ligase complex binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="38"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88175.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="424" mass="47982" checksum="AECCF0C3D92EAAC8" modified="2012-04-18" version="2">MISHQLGATPSSSKKALCYPLGLDRRLQLSSSTMSACSRLPSELLVEIFNWLPPLSHSSDRLSVCSVCRSWLGPAQIVLWRSLWLRSSSSVSSLAETVNQKPELAKLPRALHFSTGDNEYLAELDADAVFTTMERLVNLEQFHVLVNYCMGDLYDSLCEELLGSPVKHIVLGADEGISRSHVQASMRFERLEYLELLRLGDLGDLRSLELEWVFSESLVELVLIQPDLPGDALRFLLSHVKGTLRRLKIHFGHLIADNAEEGSTTRITEEDLETALMESGTDLRHLELCWPYSTRPFLNEAVKGLFSLEDMYISGGICDPGLIDLNCQSKLERISFDLRTGTEPPKSICRLVEPSARRSNLTKYHVRTFFDDPSWEQSVLKMVEMRDQDQDIPLTDWSKECIENWQKLSKKLEASGIKLTADFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVU3</accession>
    <name>E3KVU3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="4">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_13996</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PTPA-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="4"/>
    <dbReference type="EMBL" id="DS178314">
        <property type="protein sequence ID" value="EFP88418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332837.1">
        <property type="nucleotide sequence ID" value="XM_003332789.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KVU3"/>
    <dbReference type="STRING" id="418459.E3KVU3"/>
    <dbReference type="GeneID" id="10540722"/>
    <dbReference type="KEGG" id="pgr:PGTG_13996"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13996"/>
    <dbReference type="HOGENOM" id="CLU_1090451_0_0_1"/>
    <dbReference type="InParanoid" id="E3KVU3"/>
    <dbReference type="OrthoDB" id="16120at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008160">
        <property type="term" value="F:protein tyrosine phosphatase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004327">
        <property type="entry name" value="Phstyr_phstse_ac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043170">
        <property type="entry name" value="PTPA_C_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037218">
        <property type="entry name" value="PTPA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012:SF0">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A ACTIVATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140984">
        <property type="entry name" value="PTPA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0697">Rotamase</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="182"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP88418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="255" mass="28815" checksum="29A2582249E0247C" modified="2011-01-11" version="1">MDLQQWLGVAIFVRLDCRRRGTLRQARWMTLPIGNPEASADVPDVNNMAQCLQSINGRYPGTARFFAQKPAPGTAPGTAWRVRGGCGQYLPSGARYLAAGTEERSLIYGLDDFFHVVYTNFLNTQREADVEGQQDRAKRRRAGRVVFRFPDFLRREGFTQSLFSEDVQETLSLNTCPILKDSPPKTSMTRTTMRMGKQRHKERSKQQGLLENSPILLSLAINFKGWPKVYAGLIKMMKGKIFAQFLAMQYISPAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KWY5</accession>
    <name>E3KWY5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_14217</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178317">
        <property type="protein sequence ID" value="EFP88878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333297.1">
        <property type="nucleotide sequence ID" value="XM_003333249.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KWY5"/>
    <dbReference type="GeneID" id="10541994"/>
    <dbReference type="KEGG" id="pgr:PGTG_14217"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14217"/>
    <dbReference type="HOGENOM" id="CLU_761027_0_0_1"/>
    <dbReference type="InParanoid" id="E3KWY5"/>
    <dbReference type="OMA" id="WSINHES"/>
    <dbReference type="OrthoDB" id="10513186at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172284" evidence="2">
        <location>
            <begin position="21"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="319"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP88878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="364" mass="39011" checksum="9F353EC6A53858E2" modified="2011-01-11" version="1">MRFACATAGVLVLGATVSQARSIAPSPWSERDSTESEAVHFGLVSQRSTADSDDWNSSSTPQNTLPLKNNDDLSAPSPKRAGSGSAQPSSASDSQDDSESSSRVHEYSQKDYSRERVTHQPSKILGLFSVRPTIQRNEDPQSTAMLLQAIEDYRGAILGESTAGPVETPPNQAEHILQPLNQVQTRPVRQTIHELNLNRNAQEVLNRTAGFRTARKSQNDTSNGSANLTEATDNVLGNLSSLSNNIPINVQNSNDDKNSSSSNKLFPAIAQANTRDLINISSLSNNINTTDPINGAVPSSLINRVTSTNDTDSDRNTNHSHYSVSHSGASREANTTFNHTVPISHTTPANDTAVPSSNSTAPNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3KX10</accession>
    <name>E3KX10_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Spt20-like SEP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_14242</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178317">
        <property type="protein sequence ID" value="EFP88903.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333322.2">
        <property type="nucleotide sequence ID" value="XM_003333274.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KX10"/>
    <dbReference type="STRING" id="418459.E3KX10"/>
    <dbReference type="GeneID" id="10542181"/>
    <dbReference type="KEGG" id="pgr:PGTG_14242"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14242"/>
    <dbReference type="eggNOG" id="ENOG502QS30">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_363743_0_0_1"/>
    <dbReference type="InParanoid" id="E3KX10"/>
    <dbReference type="OrthoDB" id="1932706at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021950">
        <property type="entry name" value="Spt20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046468">
        <property type="entry name" value="Spt20-like_SEP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13526">
        <property type="entry name" value="TRANSCRIPTION FACTOR SPT20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13526:SF8">
        <property type="entry name" value="TRANSCRIPTION FACTOR SPT20 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12090">
        <property type="entry name" value="Spt20_SEP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Spt20-like SEP" evidence="2">
        <location>
            <begin position="23"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="709"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="387"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="448"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="468"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="516"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="742"/>
            <end position="778"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP88903.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="785" mass="86642" checksum="08FE15DF17A59B6B" modified="2012-04-18" version="2">MFRQPGIYNLTRTIRSILKRYRKESPSLTMHLYSAGFRLGAKDSTLHAYDGEMKHWMDCIREQKMPNELLDVLDELGIRFYDGCLIVEVHDHRSAQQQPEQTQSTTDHHHHHQSTTANPQQPPVAAHQRASNNLLHQNSNSHQPSTTTDLPNDQVSVHRLVLTPTCETLWRDILLMNEAKLAQLNRGTKGTSSSSSSSEFKWGPITEEEAVELEAAILDRTAPALCLSPSIITSRIANQMLSETTLQPSTDKKKSRKRPLNSGEKEAMRRQRERQERYMHIADESYDQSFAPTFSRIDLIKRLRTTRPVITAPQEPEKKWNARDPNQDALKGFIPQMTVAPSDLHLPGYSLRDPKNESQQATKPVAGSSSHQPSNSGRPSTPPPASAAPSATTVPAPAPTGKKTASKKKAVERSETGSPAAGSLAPLKKPPKKPKKQVELTPEAAALAEEKRKKQAEQRKIQKERKLALAAAAKAEAAQKAAAAASSGSSAPTPSNRETSTAAAPPATENSIPVPNRDHPANQSTEYHHHQQADISISVPDSIPVVTGDSIPVPNQFASNNHLNSSIPVPNLPILSNHTSPAPNHGIPVQQDVNVNSSSIPVPAFNHSIPVPSPSHHNSIPVHQGIPVGNELLPMSAPDSIPVPSPGNDINCFPVPNQAHHPRMDQLASIPVPQSEPQLWNPAFANFRPPSNPNHLHQRAPDIDIDVHLRPSQPPAIPSLTSIEVPNIPIHPPALHQPLLTQQEHQQQQQQQYHHQQQAHQQHQHQQQHQQQQQQHQQPFHNWSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3KYB8</accession>
    <name>E3KYB8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nucleolar protein 9</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3 4">Pumilio domain-containing protein NOP9</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_15488</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178321">
        <property type="protein sequence ID" value="EFP89309.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333728.2">
        <property type="nucleotide sequence ID" value="XM_003333680.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KYB8"/>
    <dbReference type="FunCoup" id="E3KYB8">
        <property type="interactions" value="495"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KYB8"/>
    <dbReference type="GeneID" id="10547029"/>
    <dbReference type="KEGG" id="pgr:PGTG_15488"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15488"/>
    <dbReference type="eggNOG" id="KOG2188">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008720_0_0_1"/>
    <dbReference type="InParanoid" id="E3KYB8"/>
    <dbReference type="OrthoDB" id="392571at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000480">
        <property type="term" value="P:endonucleolytic cleavage in 5'-ETS of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000472">
        <property type="term" value="P:endonucleolytic cleavage to generate mature 5'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000056">
        <property type="term" value="P:ribosomal small subunit export from nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040000">
        <property type="entry name" value="NOP9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001313">
        <property type="entry name" value="Pumilio_RNA-bd_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13102">
        <property type="entry name" value="NUCLEOLAR PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13102:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22493">
        <property type="entry name" value="PUF_NOP9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00025">
        <property type="entry name" value="Pumilio"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50302">
        <property type="entry name" value="PUM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="Pumilio" evidence="5">
        <location>
            <begin position="166"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="269"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="273"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="300"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00016427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00030932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00031929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP89309.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="713" mass="80315" checksum="970ECDC27AB040CF" modified="2012-04-18" version="2">MPREFRKRGRGGNKQKDGEDCDGNQVAKQPKRPKVTTQDVMEIAQGSEEDDTQNQGRFNADIKDPDVADFPQLTPDLRAYWKEIDEKLQEFERLGLGAYHSHSNKANQNDEAEDDDEDERQLLLRSALTELGGHELALASDPETSIILERVIYSMDGFAKRVLADRFIASFVDLCNHRHGSHVLQTLLSSSADLLDEEAPLNQSNSKKKAQDDSEQDENLPLMSDLLSQACDDILPHLDSIVSSPSGSHVLLVILRLCFGKPIGEENVRSKKSTQWRSKQGSLRSVFHAKPSSSSEPSSTPQPAPSTPADPPRRVPKKLRQKGQLVYAQMRRNWSDKNRLGGVRSIASNPSIVVLLQLIIELEFDRGEAEVPDSLTDIILDGLLQTSFSSERSEFVEVSLRDVTASRVIEGILSRLSPALFSRFHSVYLVGRMGHLCNHPVANFVISRAVSRLDLENIRPAIEEFRPKLVDCIDNYRIGFFKALVERSATFEDTVHKEVIEMILKAFGIDGESDYRWIFPCLISLMRMDYFRKTKMFNLLDSAAEVTLEQVKLPESNVQGSVLLQSILQTLPRSLDAIITKGLAQLPIEVIIGISRDPIGSRALDALLKSPSITPSIRRHFTLRFLGNFHRLADDRIGSHVAETFWTISDVYLKEKIAASLVNQQIFLQKSSFGHFFLKKLELPLFERQREAWKSKMLLKFPRLIPPSSAVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3KYC2</accession>
    <name>E3KYC2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15492</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP89313.1"/>
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    <dbReference type="KEGG" id="pgr:PGTG_15492"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15492"/>
    <dbReference type="HOGENOM" id="CLU_1918116_0_0_1"/>
    <dbReference type="InParanoid" id="E3KYC2"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="132" mass="14486" checksum="D3EE8FA8B522598F" modified="2011-01-11" version="1">MEWGHSQEDEPKQTSIIKGIGVKKPCGISPFKSKLGLNICPKVQDGRNRNDPEKLESVGGGSMQTECNRHEDGGEVKVSQNLKNVRKQKDCAVHISLLGGGSVEDESLNGRKLELMGDKLGRLGWSFISRIK</sequence>
</entry>
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    <accession>E3KZD4</accession>
    <name>E3KZD4_PUCGT</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    </protein>
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        <name evidence="2" type="ORF">PGTG_15622</name>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
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            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <dbReference type="KEGG" id="pgr:PGTG_15622"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15622"/>
    <dbReference type="HOGENOM" id="CLU_388892_0_0_1"/>
    <dbReference type="InParanoid" id="E3KZD4"/>
    <dbReference type="OrthoDB" id="2507159at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216:SF65">
        <property type="entry name" value="PAXILLIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216">
        <property type="entry name" value="PAXILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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        <location>
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            <end position="137"/>
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    </feature>
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            <end position="203"/>
        </location>
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    </feature>
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            <begin position="676"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89659.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="686" mass="75292" checksum="1C398E75A686BA3C" modified="2012-04-18" version="2">MSSRHDGSPTGYLPNMGQSTAPIIPSGGPPPLFHPSSVGSMSPSSPPPHKTLILHRGLLPGELYSDYGYSNQDVDFAAHGVPPSIPHFVYYNLPPPSEPHPPALTTNNQLPAFNPTPAESAPSNTTGAHLTNSEVRNSTTSEISPSLPPPLSIFENTPSQTYSRPVLSSAPSHCTPSQTSRSCRPLPKSKAPRKANITRKKKTALTQTAINPQPSNPIPDPPPPAGPLADLHDRQSKKRKCLTTTDSNQPEAQSEEALLKLSEAQLAIQARKTSKRAMSDDDRDFFAEYQHYLRKLLIIRAIERGVSMPMVDGFLGKRMVIKQPTPWNNFQKTKEARDIFRGQPDGVAKKNGMLEVSSKWHGLSDEEKLEFAKCVRSVGSNARPANTEGTGNNPDEVDDADEIDAAGEVSMRQTVSLKRAADQVQNFMDDWVARAVHIAKTTNCEMVMFAVSRHLGSHAFQLAKWTHGATRFVTGAKALDGLKHYPARMQAYLTGYEVAQVAKINSKKGCAVKKHPVSISKRMAALIEEKTQGTMVKWPWTKTEKKLARMKFRLELLPGARSKREWLMEPSRGLTSAPEATLHNDLDKKLIELVYDPTIPDDPSSKPTARGRRRQPPSGSRKGRRSGPLSGSSTIGSPKSQQTMSDVSNSPASQRTMSEESDIDHESDVSHRSLDESDEEDDDDNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZI7</accession>
    <name>E3KZI7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="2 3">
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        <lineage>
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            <taxon>Pucciniomycetes</taxon>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178325">
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    <dbReference type="KEGG" id="pgr:PGTG_15368"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15368"/>
    <dbReference type="HOGENOM" id="CLU_060386_0_0_1"/>
    <dbReference type="InParanoid" id="E3KZI7"/>
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    <dbReference type="Proteomes" id="UP000008783">
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <end position="199"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="235"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="212"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="235"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89712.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="331" mass="36335" checksum="4AABA424C9A2555E" modified="2012-04-18" version="2">MFFEYGFPQDRCEIAPDSPERFHERAVVGLVEGHWLDACNQPINQKLMMDSSNRPVHANHILLCEPSPVDNINSCLPRAGTAFERGLESQPEHEALRSIAENGWKVVYKVMRARFGVKGFEVVYAFDDNHPFGLDLPVGHRPCTRLSGQPPRRKKVKTSASGGTSEVGIADLTLSQSHNVSNGATPPAPRAQNHSYSTLTPASGSNIIRQYSQSTHPSSTNPPSSRLVSPTRPKQSQWHSAQSLLTWWLAHNKLSTAHLSAAQNSITEFLSSLSPALTPYGTHFKWLGVQSPVDLLGLIDSGGEEELEVFLDEIDVLVGSVLGVKGPRMKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>E3L0S4</accession>
    <name>E3L0S4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GYF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15976</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178329">
        <property type="protein sequence ID" value="EFP90128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334547.1">
        <property type="nucleotide sequence ID" value="XM_003334499.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L0S4"/>
    <dbReference type="STRING" id="418459.E3L0S4"/>
    <dbReference type="GeneID" id="10546186"/>
    <dbReference type="KEGG" id="pgr:PGTG_15976"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15976"/>
    <dbReference type="eggNOG" id="KOG2950">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024456_1_0_1"/>
    <dbReference type="InParanoid" id="E3L0S4"/>
    <dbReference type="OMA" id="GENTNFY"/>
    <dbReference type="OrthoDB" id="331341at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039905">
        <property type="entry name" value="CD2BP2/Lin1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035445">
        <property type="entry name" value="GYF-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13138:SF3">
        <property type="entry name" value="CD2 ANTIGEN CYTOPLASMIC TAIL-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13138">
        <property type="entry name" value="PROTEIN LIN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55277">
        <property type="entry name" value="GYF domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="362"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="453"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="371"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="456"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="567" mass="62789" checksum="3818369914BF2731" modified="2011-01-11" version="1">MANKRSLDPPLDQSPSSLNQAKKVKFGTLPPNSGSSKARNKKPASSSSTFEPIDDSTDLQFDELNQVGKIKKGNVKVDGYDSDSSTENTEVRSSKKKKKQSKDEMDEDDIFGDEKEADGDGRKDKPKFEKIDVGLKVKKGAEAKDFLDLGDIEGQEFGKEEEEEEEEEDEEDEDGGGGGKKKNRARNQQQEEDDSANDYSEEEEDFVPGDEFANADDAPRARRKSKKGMGFLLSKFNMAEELQEGRMAADGSYVASAKDPEAVHDNWLEGVNSKKTIKQARDAKRLRDEQLRSKVEKEESMASLRTRKECYIALLGLLPVGDGRTVSQILCHLGNEKRALQGTEKKPKKKVSVRKSAIDSLVSDTMEVDEESKTNNGDHRERSADGKPEEENRSTSQQTKSEEEVKLDRRIGEITDLASMLMGTHGELDIYEMSHGSITGILKSEGLVPRDWLPPSSQDISITSSSGVQSDAGTSQPARKSLISRPSIASALAQSPQIYYKFIHSSPPTSNPSESEAPVYGPFDKSTMLSWAQQGFFGHDFERILVKLDSSLTGNATWGPWNQIFNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3L1Q0</accession>
    <name>E3L1Q0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Large subunit ribosomal protein L18Ae</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16062</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178333">
        <property type="protein sequence ID" value="EFP90475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334894.1">
        <property type="nucleotide sequence ID" value="XM_003334846.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L1Q0"/>
    <dbReference type="STRING" id="418459.E3L1Q0"/>
    <dbReference type="GeneID" id="10530371"/>
    <dbReference type="KEGG" id="pgr:PGTG_16062"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16062"/>
    <dbReference type="HOGENOM" id="CLU_080773_3_2_1"/>
    <dbReference type="InParanoid" id="E3L1Q0"/>
    <dbReference type="OrthoDB" id="1294322at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023573">
        <property type="entry name" value="Ribosomal_eL20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021138">
        <property type="entry name" value="Ribosomal_eL20_eukaryotes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10052">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01775">
        <property type="entry name" value="Ribosomal_L18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160374">
        <property type="entry name" value="RplX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL20" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="83" mass="9967" checksum="42418E23EA4142F9" modified="2011-01-11" version="1">MYKEFRELSRVDAAQACYQDMASRHRARFRSIQILRIAEIEKASDVRRPNIKQLLVPKLRFPLPHRVVKYRSKFLATRPSTFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3L337</accession>
    <name>E3L337_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_17234</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178339">
        <property type="protein sequence ID" value="EFP90962.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335381.2">
        <property type="nucleotide sequence ID" value="XM_003335333.2"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L337"/>
    <dbReference type="GeneID" id="10528377"/>
    <dbReference type="KEGG" id="pgr:PGTG_17234"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17234"/>
    <dbReference type="HOGENOM" id="CLU_000202_1_0_1"/>
    <dbReference type="InParanoid" id="E3L337"/>
    <dbReference type="OrthoDB" id="1562405at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019441">
        <property type="entry name" value="FMP27/BLTP2/Hobbit_GFWDK_RBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019415">
        <property type="entry name" value="FMP27_SW_RBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019449">
        <property type="entry name" value="FMP27_WPPW_RBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045167">
        <property type="entry name" value="Hobbit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15678">
        <property type="entry name" value="ANTIGEN MLAA-22-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15678:SF6">
        <property type="entry name" value="BRIDGE-LIKE LIPID TRANSFER PROTEIN FAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10344">
        <property type="entry name" value="Hobbit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01214">
        <property type="entry name" value="Fmp27_GFWDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01215">
        <property type="entry name" value="Fmp27_SW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01216">
        <property type="entry name" value="Fmp27_WPPW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="domain" description="FMP27 SW motif-containing RBG unit" evidence="4">
        <location>
            <begin position="1538"/>
            <end position="1642"/>
        </location>
    </feature>
    <feature type="domain" description="FMP27/BLTP2/Hobbit GFWDK motif-containing RBG unit" evidence="3">
        <location>
            <begin position="1660"/>
            <end position="1823"/>
        </location>
    </feature>
    <feature type="domain" description="FMP27 WPPW motif-containing RBG unit" evidence="5">
        <location>
            <begin position="2102"/>
            <end position="2633"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="676"/>
            <end position="706"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="815"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1002"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1459"/>
            <end position="1532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1892"/>
            <end position="1911"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2265"/>
            <end position="2285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2369"/>
            <end position="2388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="2491"/>
            <end position="2514"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3058"/>
            <end position="3091"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="3221"/>
            <end position="3416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="139"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1459"/>
            <end position="1472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1488"/>
            <end position="1514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1523"/>
            <end position="1532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1895"/>
            <end position="1910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2265"/>
            <end position="2277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="2372"/>
            <end position="2388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="3067"/>
            <end position="3081"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="3226"/>
            <end position="3244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="3245"/>
            <end position="3283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="3290"/>
            <end position="3309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="3369"/>
            <end position="3378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP90962.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="3416" mass="383642" checksum="631009545374DAA6" modified="2012-04-18" version="2">MSSEPSLSGNHNQTKISSSFGPFRRSLAFSLTLAVILTSLFYLLAPKLIRKYARRFKIGQIGFLSLKDLEWTSSHQNISTKHQDGFTTDEDQSDQNEGVKITVKSIGLRLGSNEGSRRQWIALRIDSPRIRIPKRRTRSNSFDSSSDSSEPEFSAAETSYRYGRSRKISGASSTSQLSGLSKSDAPPLQFLKTLSHKSKQATSSILRHLPAQAHPILRFLRKLLRLARLEIVRPLLNKLNRFGRRLSWIISVFGVEVNNIEVDVSKICRVTCTIKTGLQLSRGDNGKICCWIVVKKLQIHKLTTVDDTDPSEEDIKRDQAAQDTTAAFSFPGCIEISASAGLDPVIGLASIWAGSKAAGYTRRFPSSPYQNYPSNMPNGSWKRYIRPRSVNVTLKISEDLQASRRTSKKTSLSPNLKASSAFISIENALAITRALPNRTKTNESNGPSISILSDEPMFSGSNFSHLEDSQQSPSKVFSTLSIIRQFQISLPSLHCYYILAGPISTPISTSTSSPGTPATQNKKIAIDLQITRFIMNLDLSAEKTGKEAARSDRNLTTHMEWFGRGTPIPFRLNSQWKDISINATLVPESERSSELIPSGPTQLLRMSEVSFVLSSSWAPRTLREKIESENFSSHASQLSLNGDHNSHMIVAEFEIGKIRGQMPIDNLLIMHMLSQSHPSGSSKLKAVPPSNSQNNHRPRNNRREDTPKFVFGLSIQSMSYDFYGPPCPPTTPSSSSLGPTFNPINNIGNSSRGNVLSIGCSSFHFNSAGEYVDLVVKRSEATRRIARTRARKNEVYIPPTLRGIRISQAHEDSILSEKRPVDADSASTCREADSTSIKLSDESFDNLRDVGPHPQAYPLPNPSHSSTQWKSQVDVFREKSSVDFFYRLSFTWQTHSIDVVFQDDQLKLDKSRHPFASFDLPSRMSSSGPYDFFSLKTIEVTSEFCFAGRNIVCFDRKPVPTIDLTLREGTVQTFIEEVSFDLWRPNVLANLVAFTQTFQKFRRQSHPPETKNESDPDSASVPRTVPIPRDIAFAIAASRLSLRVAGFDPKRDSTIARGTQLVVSNALIECFRQSNAQPGLYGSPHRSRLDLQEDIRVQANAQLVHNPEFESCLLKCAMKKIEMFALPDLTGSLHSNRFSRFESPSHQRMFHYRSPSLWDDKARFGFRNHHDPQSPVDSTLHEPQDILSENKEVSILEVNRLACRTTLQILKQAKRGEDELILAVETGLITFHIDAFHIYCALMSISTLLNLAKKVSSNSPESAKRSAKSSTRLPIGIRAEISKIHFHMFLSHQVPIFIEIRRVSFQKSSKLGFDARWDILLLAGRSVTSPGRWDDLLKVKTCKVKLVQINPSNSPSDPALVNRGWHPFIVLIEGDAARLRIPFKFVIAEVIENVSLLVKTTKQLIYQFIHGGTGSVLQPEVEAPKRLPEIRIKFRIVCVQAEDDPFENKLNAIRRAGKEEVKDRLARDESFEQKVQGMNGPKNYPPTKETKPRQSWESHRHSERGSTDSPRNDDSSSFDSSSYLGRTGTQSPHVSIAQAAQRLNEYNGAAWIKRIRNALAEQERQEDAIQRQLYGHTATKLTDVMPVPMLPVTKATPLVRVVFNSIRIALYKADFEKDENGLMNYLHEVGKGLPKETKFSLIVPMHISWQMEGATMRLRDFPLYLFSLPRPQAQNGHQQERELSQYTWQFESDFVLAEEMCGVESIRAVQSVVIPSHHSSDGSVYTLDIPKSVMPVKSYADPFIKIKSTAPVQLGWGNSMQPTVQDMMRVLDSISKPPVDLSERVGFWDKVRLVLHWKVDVSFAGSKADVVLHLKGSRDPYELLGSGAGFAKVWRGNVKILLGHQNDEQEFLQIKSDQFIIGIPNLKDHINNAATGSAPMATATPAANYKRVYQRRSQGSGSDESDTETLSPRETEFIKIVGKLTNGVRWGMGIVLERACSDSLDKTCNCHGPAFHRKCRIFSFKPHYQVVTKTPQYGRAADGSPADSYEDFRSDFIHFSISLTSPTESGSRQKRPTGQNSLYFSAESFTHFWCWWKTFDSALALPIRQGNLFPSAQAPSKKFGRHVATIKYRFGISPLFLAHTYRYESAPDWVRGKNVVLGFKAKISTFNVDLHSRAVETTIRKPEMKEPKKLIRKAFYQAEIECQDVEIRALSAVFQTPRKSTYANDIGLTADQDESETNSIWEDSLEDESEFLVNPSGEEGGFSIYPSKLGKKDMEWIDLNDFSDIFFWPNSSGAQQPKIKMFDCLSCPRISFLRRPSSATSFQVSPTNNNASDGESIADGQPIERTKFGKENTHTCFIGKSEDPMKVQVGLIDARLKELSEKKYHCLVTAGSYHPQRPEDTMMLLQIEARMQTLRELKATMLRDKGNANRNSQSEDLGNQTNGLDCSESWADGERFLFEMAKQISDENSEWSNRLLVHNPSILISNSIRDILLKYYYSSSQRRAFMYHISARAVKFILDLAEQESSIPAPKPKQDFNKRRSRMRSFDYQKYDGRSNSGTGSKPQPKFPNEDCPVSPLRACIEEQAEQDVPDEFILDPADLVLLLRPQIAFRSEVDDISTVILTAFHAQFKSFEVLDPSHIDDPVNATVMKRNFGTVKGFQMFYPCNPSSSSSLHPAPGDQDFQVPIEVLVDLRLEPWGFDRLIGRCTVNIANDAFNQLRIKRRSAGADGSFSSATQPHLQTSTDLLRVEMADAVSVHATATHYRAIYNVITDLCLYIDPAQKKRNAALETMQFAYDVDDLEGMADQIQQQQARIRLYRNKLDEGYVDLNVFDEVRMAALTQCEYQLWIHASDLNLMVEAIRRSQASRLGRSKAEKLPGSQLLGHAKSITWHMLADSGEAIAKLSVTDTRFESYTLPDTSVSNRLYVQDMLALNISADSDRQFDEILSRYEPYNPLGPAVNSKQFLMVLWKTLPPIAGISIVESFLLEIHPIRLQVEHAIGVKVHDYLFAHKSTSVESENDSDEPSNTVETMNVPVVEGETKKLNKKRSMLLNLHQPKSRVVTPDLPDIKRRTGNFSTLPSQESLYDLHSVSGRSRTTESSGFLNADWRSSFGANQSTSGGTLSSRASRAVSVRSNSSDGRRQGASMGATPTVLMNWNKQKTEDATEMKKRAQLYKSFLFIDVAPTILCVSYSGPKYPDIFDLVVKVPPFHFESRTWSYSEFFDEIRRTCVSSLFKQSPSILGQIITTARKNKSVPKLMGQKMASGFKFKKRNLFERVNSQPTNTRHTSTSSLSSTLAGTTIDQGSSATSPDTNHSRSPYLSTDTSSSFEGYHSQSTQDATPRPAFFNQSPKQPYTYSSNGNSPTLGSLDLESCESINDFPEPDHSPPALDQRSRSYGGMDRPSPYAPQSRRQTHMRLSASDASHFHPHQPTRQRRQSEGPLSSHHHHHHLSSTPEPMPFNSMFFTAPSPSHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3E7</accession>
    <name>E3L3E7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16934</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178341">
        <property type="protein sequence ID" value="EFP91072.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335491.2">
        <property type="nucleotide sequence ID" value="XM_003335443.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L3E7"/>
    <dbReference type="GeneID" id="10528651"/>
    <dbReference type="KEGG" id="pgr:PGTG_16934"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16934"/>
    <dbReference type="HOGENOM" id="CLU_044243_2_1_1"/>
    <dbReference type="InParanoid" id="E3L3E7"/>
    <dbReference type="OrthoDB" id="2509372at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="212"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91072.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="243" mass="27660" checksum="014141C54B6ED243" modified="2012-04-18" version="2">MTFKSEGYYTTGPSNKQERASPTPSVDTNNGDSDHTTPSQIDQNSEAPSQLQRDLVAAKELRARVENLQMTTLKRKETATASREEPPQNMEKSSAMEIDEIDSECSEATPKRPITPEIRVLSKEDQIKLRVKEHVSLWRQCQVATREGPTEKLRALLTTAQESQKVLQKLIDNEEIESYVKGWNPWDEKKLHFPSPPKKNLMKFKRNEPGKKQSSSSTNFSDPAKWRKVTDLLQMASGLYQNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3K0</accession>
    <name>E3L3K0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_16870</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178342">
        <property type="protein sequence ID" value="EFP91125.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335544.2">
        <property type="nucleotide sequence ID" value="XM_003335496.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L3K0"/>
    <dbReference type="GeneID" id="10528705"/>
    <dbReference type="KEGG" id="pgr:PGTG_16870"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16870"/>
    <dbReference type="HOGENOM" id="CLU_2237942_0_0_1"/>
    <dbReference type="InParanoid" id="E3L3K0"/>
    <dbReference type="OrthoDB" id="10321928at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP91125.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="105" mass="11240" checksum="D75D6EF562471036" modified="2012-04-18" version="2">MAPFGRHTGGNIETVGKRKKVCKNLKGGEEQAVLDLGGLVIATLRKTGSTDRPDRLSDGSIGDAHSRQLSAFRIKCLVLVTHSLWQRNCESEDTRAAGLTKGPGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3L405</accession>
    <name>E3L405_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178344">
        <property type="protein sequence ID" value="EFP91280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335699.1">
        <property type="nucleotide sequence ID" value="XM_003335651.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L405"/>
    <dbReference type="GeneID" id="10529028"/>
    <dbReference type="KEGG" id="pgr:PGTG_17137"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17137"/>
    <dbReference type="HOGENOM" id="CLU_1886781_0_0_1"/>
    <dbReference type="InParanoid" id="E3L405"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="135" mass="14543" checksum="583580E13B2C1DC5" modified="2011-01-11" version="1">MSTTFGFEGMSKANSGGESASERRVQRIDVVFNLGRVRLRATSPTNRCRVQLGLNVALELEVGLGVGSDSRTGLSGVNFRNGVTTEVQAMPADNLRIACNIGIRSGNRCDADSPHRYSDADDAMRGRIAHTDAMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3L460</accession>
    <name>E3L460_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GH16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_16989</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178345">
        <property type="protein sequence ID" value="EFP91335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335754.1">
        <property type="nucleotide sequence ID" value="XM_003335706.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L460"/>
    <dbReference type="GeneID" id="10529165"/>
    <dbReference type="KEGG" id="pgr:PGTG_16989"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16989"/>
    <dbReference type="eggNOG" id="ENOG502S2A9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090399_0_0_1"/>
    <dbReference type="InParanoid" id="E3L460"/>
    <dbReference type="OMA" id="IWDASEH"/>
    <dbReference type="OrthoDB" id="4781at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006030">
        <property type="term" value="P:chitin metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050546">
        <property type="entry name" value="Glycosyl_Hydrlase_16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963:SF22">
        <property type="entry name" value="GLYCOSIDASE CRH2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10963">
        <property type="entry name" value="GLYCOSYL HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00722">
        <property type="entry name" value="Glyco_hydro_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173098" description="GH16 domain-containing protein" evidence="4">
        <location>
            <begin position="21"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="5">
        <location>
            <begin position="42"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP91335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="281" mass="29904" checksum="5AABCC17EBC98D20" modified="2011-01-11" version="1">MNRIQLSLLFLAVCSGLSQALGSPHKSHKSAPRKVVAPKSRTVSQCTPMSETFSSSGLPPSWVQLSTNPQAHKMNPGGGVNLALNPPVGPVLQSANGKTNDKLGDGSTLNSTYSFLYGKVSLTLAASDVPGAVVALVLFGTTTADEIDIEILGGDAKHWQTNVFRPAPGETEPLYGVFGGVHDYANGGTTGGIHTYTVDWSPSGITWSVDGRPVRTLTPDQTLHNGQRHYPSARSRIQLGLWDASSPEGTSDWAHGPVPWAKRQRKPVTALIKSIKVECPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3L462</accession>
    <name>E3L462_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16991</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178345">
        <property type="protein sequence ID" value="EFP91337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335756.1">
        <property type="nucleotide sequence ID" value="XM_003335708.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L462"/>
    <dbReference type="GeneID" id="10529168"/>
    <dbReference type="KEGG" id="pgr:PGTG_16991"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16991"/>
    <dbReference type="HOGENOM" id="CLU_704256_0_0_1"/>
    <dbReference type="InParanoid" id="E3L462"/>
    <dbReference type="OMA" id="WFNSASE"/>
    <dbReference type="OrthoDB" id="2501603at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="342"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="438" mass="47513" checksum="125095F5C1A6E3BA" modified="2011-01-11" version="1">MSSSKFNTVYESLPPPIPAAIEIIRHILVSPSSLFEAPLSRPVLERLQWIKPSLEDERESHLYFNNMVDHGASGRAHELRMELIHAGPSAWHFCAPSEVHYKADERCLDSDGLCEIFSRVTVWKARDSDAEAVQAREKLALILKLECDPPEPADPTKKGSWDHLSWKYFDLQDLVTPGLDTRPWFDSPRQAALRPPIHQNSGGPKDREQSGDEDADYWAGFSSRSDDSSMTSGSDGRGQARIPTDPALAARIRNTPLYRSKAPSVCSPAASDDHVQPQGTPSLGGPSGPIDLRARIARTPLFKRAPSVEDCGPLNSALPGNPSTAPDLLRLLDAARIVNLPSDPPVRPAPPPTLPLHPPLKSSLAPLPQPSGASSTSQNALQDKILDSLRATFHLYRLCLHNPASPSSSSSSALSAGESFVALAHRVVMQDAAGLNQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3L4L6</accession>
    <name>E3L4L6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17741</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178347">
        <property type="protein sequence ID" value="EFP91491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335910.1">
        <property type="nucleotide sequence ID" value="XM_003335862.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L4L6"/>
    <dbReference type="GeneID" id="10529492"/>
    <dbReference type="KEGG" id="pgr:PGTG_17741"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17741"/>
    <dbReference type="HOGENOM" id="CLU_489274_0_0_1"/>
    <dbReference type="InParanoid" id="E3L4L6"/>
    <dbReference type="OrthoDB" id="2503277at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="170"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="297"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="557" mass="60828" checksum="0D99909D55BCD0C2" modified="2011-01-11" version="1">MKPTTKNRRTTTTRLTSSPHPSSSSSASSSSSSLPRTPKPHNTLLDSILSSPRQSHTTKKTETQITPNKLNRALNNLLNQPRGHPSHKQTAMNQLIQTLSTLRSKHHIHDHLEQPYSKRIKIQAAADGSASLVLPSMQPTLDPSHYYYQHLHYPQPTTIAPATLFLSPSSSSSSNPLLASSSETNQHSPLVSATTQEYGRVIMKKDALINKLGLEAAQRAIRKARSQRKPYRPSANHPPSSSPPSSPQTSCWRTGRIGKWLESADDDSEDEGDRRTNLPRGRDLMGSLSKLAKEESSTSAQPSSSSTDQAGNDPQSLFWSPIKGKQKPPGMPATKRAPHSASIAAPIDSQQILERIGRMSALEMGIRWPLDRPSTPPNQQSSFFHIDELNAARGHRHPADRLAPFSQLSFGSQSGTALQTSMLKPGCNLSQAFLVRAASPTILLSAPAPADENRSPLVLAPQPHLSPSLPARPHQNDFSPCSPLRHDLLKLIDQHQGSARLDSPNPITAFSSLQSTATHEHRWLADLDTNSLANLNFDNPFSSPTPDPPLEDFLGYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3L7I4</accession>
    <name>E3L7I4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_18507</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP92509.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003336880.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L7I4"/>
    <dbReference type="GeneID" id="10538504"/>
    <dbReference type="KEGG" id="pgr:PGTG_18507"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18507"/>
    <dbReference type="HOGENOM" id="CLU_3015247_0_0_1"/>
    <dbReference type="InParanoid" id="E3L7I4"/>
    <dbReference type="OrthoDB" id="10274669at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP92509.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="56" mass="5938" checksum="7C57BAEB2E72F820" modified="2012-04-18" version="2">MNCLEADQKSSLESALRGLLHSGRLSATGANGLHSPNLMKPRSISIFLLTNTDKAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8D3</accession>
    <name>E3L8D3_PUCGT</name>
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        <recommendedName>
            <fullName evidence="6">Velvet domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_18587</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="protein sequence ID" value="EFP92808.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="XP_003337227.2">
        <property type="nucleotide sequence ID" value="XM_003337179.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L8D3"/>
    <dbReference type="STRING" id="418459.E3L8D3"/>
    <dbReference type="GeneID" id="10542521"/>
    <dbReference type="KEGG" id="pgr:PGTG_18587"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18587"/>
    <dbReference type="eggNOG" id="ENOG502S1B4">
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    <dbReference type="HOGENOM" id="CLU_022491_0_2_1"/>
    <dbReference type="InParanoid" id="E3L8D3"/>
    <dbReference type="OrthoDB" id="1746739at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005992">
        <property type="term" value="P:trehalose biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Velvet domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021740">
        <property type="entry name" value="Velvet"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037525">
        <property type="entry name" value="Velvet_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038491">
        <property type="entry name" value="Velvet_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33572">
        <property type="entry name" value="SPORE DEVELOPMENT REGULATOR VOSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33572:SF3">
        <property type="entry name" value="VELVET COMPLEX SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11754">
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    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
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        <location>
            <begin position="17"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP92808.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="247" mass="27416" checksum="83E3420039E33E8C" modified="2012-04-18" version="2">MYASTSNYNPPADRPRSSHERHYELRILQQPVRARMCGFGDKDRRLLTPPVIVELLVYDSHTGMHINADEVDTSFFVLNADLWDSRGTNELNIVMHPVFCNPESTANYQSSREDQAPVPGPSSKSAISNIIHPGGTSSGATPPYYPRPALDSSARPNGEMAYTRNLIGALAQSASKLKGMDGQPGIFFIFHDLSVRTEGIFCLRFTFVVLGNHIHNNDQPTDTLATTFSEPFQCPEGEVCSKSQFFF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8I0</accession>
    <name>E3L8I0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    <organism evidence="2 3">
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        <name type="common">Black stem rust fungus</name>
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        <lineage>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="E3L8I0"/>
    <dbReference type="GeneID" id="10542662"/>
    <dbReference type="KEGG" id="pgr:PGTG_18919"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18919"/>
    <dbReference type="HOGENOM" id="CLU_785592_0_0_1"/>
    <dbReference type="InParanoid" id="E3L8I0"/>
    <dbReference type="OrthoDB" id="2505454at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
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        </location>
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        <source>
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    </evidence>
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        <source>
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        </source>
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        <source>
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</entry>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP92864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="202" mass="23776" checksum="C9C4EB426915E65C" modified="2011-01-11" version="1">MLAAELTPGIIGYQDSISKASAKIPIELRAEEKLQQDELERFHQEREANKRDEYVFRLRLLALLDNENKEISDKISSIRYRGDLILICNHLEELHQLLKLKLYKKRFGFLAARDCRLPRKIKGPHLQMGTGERAELNQNKWDDVIDICIIRKFHQLQTMLNGFISNHNEQSYVFHWDMKLQAIETIFLMGPLKVCQNLHYMA</sequence>
</entry>
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    <accession>E3L8N0</accession>
    <name>E3L8N0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">NADP-dependent oxidoreductase domain-containing protein</fullName>
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    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
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            <taxon>Puccinia</taxon>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178376">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19034"/>
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    <dbReference type="GO" id="GO:0045290">
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    </dbReference>
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        <property type="entry name" value="NAD(P)_OxRdtase_dom_sf"/>
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    <dbReference type="PANTHER" id="PTHR42686:SF1">
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    </dbReference>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="142" mass="15431" checksum="C847B2BE61117367" modified="2012-04-18" version="2">MSYVGLTLQKSALSSYLPYFQAAGFVQIISASPLGNVLLTTQGPPDWQPAPQALCQVIKEVVKSIQTTHHLSIERISLLYSMYFPHTVIGFSSVDKVKAAFEVLGEIQNPKDASSISSAQASVQDALKSSGYLNWSWPLPSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3L905</accession>
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    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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    <gene>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178379">
        <property type="protein sequence ID" value="EFP93030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337449.1">
        <property type="nucleotide sequence ID" value="XM_003337401.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L905"/>
    <dbReference type="GeneID" id="10543006"/>
    <dbReference type="KEGG" id="pgr:PGTG_18871"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18871"/>
    <dbReference type="HOGENOM" id="CLU_094315_0_0_1"/>
    <dbReference type="InParanoid" id="E3L905"/>
    <dbReference type="OMA" id="MISCCTY"/>
    <dbReference type="OrthoDB" id="3349852at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051285">
        <property type="term" value="C:cell cortex of cell tip"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009571">
        <property type="entry name" value="SUR7/Rim9-like_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052413">
        <property type="entry name" value="SUR7_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28019">
        <property type="entry name" value="CELL MEMBRANE PROTEIN YLR413W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28019:SF2">
        <property type="entry name" value="CELL MEMBRANE PROTEIN YLR413W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06687">
        <property type="entry name" value="SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="208" mass="22615" checksum="6D53E5A4F7CF9B61" modified="2011-01-11" version="1">MANPVASAGGGLAFVAAILFIFVGIANFTLDQIVPRHIRSLCRLYWPLGPTVLHGERLGLQVRPTAAILEDVPNGLRSRATNLFNDQYNTDNYFGVWSRIGFYFLFIAACLVALILLFSLIQARGPGRLGSLNLSVISLLAFISNTIGTVIWTAIAGILKHQLSRDTIGMSASYGNAIWLLWAIDGLLLFASFGYLGGCIRGRRSQVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>E3NXP6</accession>
    <name>E3NXP6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="7">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_20299</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="7"/>
    <dbReference type="EMBL" id="DS989960">
        <property type="protein sequence ID" value="EFP94345.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338764.2">
        <property type="nucleotide sequence ID" value="XM_003338716.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NXP6"/>
    <dbReference type="STRING" id="418459.E3NXP6"/>
    <dbReference type="GeneID" id="10527475"/>
    <dbReference type="KEGG" id="pgr:PGTG_20299"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20299"/>
    <dbReference type="HOGENOM" id="CLU_011478_1_1_1"/>
    <dbReference type="InParanoid" id="E3NXP6"/>
    <dbReference type="OrthoDB" id="10261556at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="42"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="241"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="588"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="629"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="590"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="606"/>
            <end position="620"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP94345.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="682" mass="76512" checksum="647C8418AFD5B568" modified="2012-04-18" version="2">MVGVAVPQALEMASDTKLKKTISESGLTKYGQECKKLQVESVLSLLRGKNTFILAATGFGKSRIPELYLNLLTKDRLGNIAGVIIVLNPLDALGDNQVEEKIRAGFSAINATAENFDATVANNILNGVYNFVYVSPEIFLDNKRFNNLYLSPAFQQKLALVVVDEAHMIYSWGLVEDGEHHIKAHVRHCDRGPFRPSYGKLSTQLLTKHKAPLLLMSATCRPAAIRIIRVPMSYPLSSCEDLLQLYSTKEETPDESLVPSLVYSGTQNLTLKVMEVLNKARGTPGEHLIPRSSFSRRFHAVTGELDKQECLTDFANGKVPLISCTLALGMGQNWNLVRQVVHMGRGDPSLICQMIGRCGRDGRPGLAVLFMEPNRKGGKNHLDDFELGMIQSDDDRMDALALTPVCLRIAFSVDNRKGYIPLSFDDPEYRLELAREIKEGFAPCMCSNCMGHMAQVLLQEIPRLNQFNFTDYNLNKCALVAQAPIQLKQKYNVAKRRETKEPTHDNKALEVLKGLLLKDFEDFFYKRLGSGGSLIPSDLFGDTQAKAIADEIQYIKNENDLRSTIHGEVLAGQLTMLLNTINRFRADPTYQPSTPAQLASSTTNDTLRKRSHEGVEGQVEKKRRQPRCSLEVMREKAQAAEERKQAQAEKKRIQEIEKLKKAARRQAQDLIRFEVKRERGMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPI2</accession>
    <name>H6QPI2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20849</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EHS63867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890562.1">
        <property type="nucleotide sequence ID" value="XM_003890513.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPI2"/>
    <dbReference type="GeneID" id="13543224"/>
    <dbReference type="KEGG" id="pgr:PGTG_20849"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20849"/>
    <dbReference type="HOGENOM" id="CLU_2484422_0_0_1"/>
    <dbReference type="InParanoid" id="H6QPI2"/>
    <dbReference type="OrthoDB" id="10436775at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="87" mass="8929" checksum="73F1C6333D1D476E" modified="2012-04-18" version="1">MDHWQRTSKWASISSSKPMTLSGGSSANPAARMVGSASPFGGGVIGNVGQIFVGLVYQAVDFNHQPIILQTVSGADGQPTIALSLNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPT7</accession>
    <name>H6QPT7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BolA protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_20935</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the BolA/IbaG family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EHS64324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890480.1">
        <property type="nucleotide sequence ID" value="XM_003890431.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPT7"/>
    <dbReference type="FunCoup" id="H6QPT7">
        <property type="interactions" value="329"/>
    </dbReference>
    <dbReference type="STRING" id="418459.H6QPT7"/>
    <dbReference type="GeneID" id="13542604"/>
    <dbReference type="KEGG" id="pgr:PGTG_20935"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20935"/>
    <dbReference type="eggNOG" id="KOG3348">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109462_4_0_1"/>
    <dbReference type="InParanoid" id="H6QPT7"/>
    <dbReference type="OrthoDB" id="4983at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.90">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052275">
        <property type="entry name" value="Mt_Fe-S_assembly_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46188">
        <property type="entry name" value="BOLA-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46188:SF1">
        <property type="entry name" value="BOLA-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003113">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS64324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="92" mass="10452" checksum="7B64552F818F9C21" modified="2012-04-18" version="1">MPISQESMEQRLIECFPMAIERKVFDVSGGCGQSYEVLIVSPEFAGKTTLAKHRLVNERLKDEISELHAFSQKTFTPEQYATVKENEQTKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQ35</accession>
    <name>H6QQ35_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">U4/U6 snRNA-associated-splicing factor PRP24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_21014</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Functions as a recycling factor of the spliceosome, a machinery that forms on each precursor-messenger RNA (pre-mRNA) and catalyzes the removal of introns. Chaperones the re-annealing of U4 and U6 snRNAs (small nuclear RNAs) released from previous rounds of splicing, an initial step in reforming the U4/U6-U5 tri-snRNP (small nuclear ribonucleoprotein) that can reassemble into another spliceosome complex; this step involves binding U6 and facilitating the unwinding of the U6 internal stem loop, followed by base-pairing of U6 to U4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EHS64663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890369.1">
        <property type="nucleotide sequence ID" value="XM_003890320.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQ35"/>
    <dbReference type="FunCoup" id="H6QQ35">
        <property type="interactions" value="668"/>
    </dbReference>
    <dbReference type="STRING" id="418459.H6QQ35"/>
    <dbReference type="GeneID" id="13542779"/>
    <dbReference type="KEGG" id="pgr:PGTG_21014"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21014"/>
    <dbReference type="eggNOG" id="KOG0128">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003925_1_0_1"/>
    <dbReference type="InParanoid" id="H6QQ35"/>
    <dbReference type="OrthoDB" id="360390at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005688">
        <property type="term" value="C:U6 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
        <property type="term" value="P:RNA splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd12296">
        <property type="entry name" value="RRM1_Prp24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000365">
        <property type="entry name" value="U4/U6 snRNA-associated-splicing factor PRP24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:003607">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034397">
        <property type="entry name" value="Prp24_RRM1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23240">
        <property type="entry name" value="HAT_PRP39_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23085">
        <property type="entry name" value="RRM_PARP14_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="658"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="733"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="824"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="11">
        <location>
            <begin position="927"/>
            <end position="999"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="573"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1009"/>
            <end position="1087"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="580"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1063"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1078"/>
            <end position="1087"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00093374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00093627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS64663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1087" mass="123124" checksum="0DA655BAC6A73F72" modified="2012-04-18" version="1">MAEVEMENAEVPPPDTDEDSPDQILNQLLEVMSALQTNPFDYSLHERNVALCQKLGAGTIEELNQARTLMSEYFPVKEDFFTDWVSDVVSSHQSPYEVDCVQKVLEIYEQASSYSFFFKIETTRLRYMIDLFYYARGLPPPAGEVIERIAESSEIDTKDPDHVTERTEEQIALESFISEERLRTLAIDVMDKTGYHLLESSAIWNMWAIHEFDNLKRNWNTDGLAQFKNMVQARLRIPHTDSPKTFQMYSNLITKYENNNYEEEMVAVNTLYSPARKLMEDREAHEAQLKKSGYSCQEYLKYISWEISRKPSHFDLAQMLFERALADHPNSVELWLEFLRYLNHHRDQHALSAAIVKRATRTVPWSGEIWACAIRTFERNNQTIEAVEGLCARAIQSKFLEHDIEALISFTIGRADFHRRRLDAYIVAGGNENAEQVKSLIDMVANVLHHGIDTTKKVLAKTGDPSCRLEKYLATFFEHHDREDDAAKVWQKATKQYRNASSPWISAAECEARRGDLTRAREYYKKSSNIKLDYPEYLLEAWLTFEHHFGALDDLEYAISKVNNLMKGISARRKREAEQNSETLAQNSSLDNTVGDDAGGRAPAETDENSETRKRKAETIAETKGGDKKIKLNTLPTEATTSTNAGEVPELKRDRENSTVLVAGLTPETSEAKISQLFRDCGRIREIAIHKISSEDVVATVEFEDRASVLPAQTKDKKMLDGVEVAVSLAWRSTLYVTNFPEDANDEWIRSKFSQFGSIFDVRWPSKRFKSTRRFCYIQFTSPASAEAALQLHNLEVSPKQKMSVLISDPTRKQTRSDNHANEKELYITCLSKYVQEDDLRKLFSQFGEIKGVRVVLDQAGHSKGFAFVEFQNEMSAKAALSMNNVELKKRRIGVTISSAKGLSLARKNTTFKDETKLSSATDHRSRSVRVSNIAEGTQEALIQQAFEQFGKVLKTITYPEKNEALVEFALEKDAGRVFLHKEPIVINDQKVEVSVPTATLKSGMAASSSFTPLLPRSTVKGKKSRIGLGHSGARPSPKPQTTKEEVSPDPKQAGSAKDVQMADQSHSNSSANAGSKTQDDFRKMLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQL1</accession>
    <name>H6QQL1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21171</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EHS62663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890209.1">
        <property type="nucleotide sequence ID" value="XM_003890160.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQL1"/>
    <dbReference type="GeneID" id="13541241"/>
    <dbReference type="KEGG" id="pgr:PGTG_21171"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21171"/>
    <dbReference type="HOGENOM" id="CLU_1338087_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQL1"/>
    <dbReference type="OrthoDB" id="10333699at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS62663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="205" mass="22485" checksum="D5269D1E578DEFC0" modified="2012-04-18" version="1">MVVDEPVIKDIKPNISVAGGPDGQLVANPVQATDAHQSSIPTAVPRMSIHNDPHPFVCVAWNVHDPELPPGLPVPFLKTTALIDLFLTSSSPDPSSHSSSAPLTSSNTSSTASSTYTPILAYIGLNHQGVQRLLLDDHNPPSALCIYTKEDVMRIFEGIAPDYVHTCSEYPPSSLAESDRQVMFFENMQWHFQNKRSTYLSTRNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQN3</accession>
    <name>H6QQN3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21215</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EHS62751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890168.1">
        <property type="nucleotide sequence ID" value="XM_003890119.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQN3"/>
    <dbReference type="GeneID" id="13541322"/>
    <dbReference type="KEGG" id="pgr:PGTG_21215"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21215"/>
    <dbReference type="HOGENOM" id="CLU_2543653_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQN3"/>
    <dbReference type="OrthoDB" id="10331377at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS62751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="83" mass="9492" checksum="B21365294255BC15" modified="2012-04-18" version="1">MINSQVVLLAQQHQLCITHLHHQLEQDVWQRTLESLFSQDSANKNQEIEDAVMLFMAMAYFGTPMPGLPCGSQQLGFHHNHGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQS4</accession>
    <name>H6QQS4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21143</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EHS62822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890141.1">
        <property type="nucleotide sequence ID" value="XM_003890092.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQS4"/>
    <dbReference type="GeneID" id="13541420"/>
    <dbReference type="KEGG" id="pgr:PGTG_21143"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21143"/>
    <dbReference type="HOGENOM" id="CLU_054436_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQS4"/>
    <dbReference type="OrthoDB" id="15595at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018852">
        <property type="entry name" value="DUF2456"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28297">
        <property type="entry name" value="FUNGAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28297:SF1">
        <property type="entry name" value="FUNGAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10445">
        <property type="entry name" value="DUF2456"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="115"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="418" mass="46105" checksum="49E49127E76DC42A" modified="2012-04-18" version="1">MPYTLLVIGTGCDAPTTAEKGHRKSRKSCSSQVDRLECLRDLEYNVLLNASMTLLQTLPFSWQVTTWGPSCKAGSLIDRRPSERLIDHDFIKKQNSSKRIQYKREILSLGIISPSPSSSSLKRSRSQQSVSRNRTISPSARKSLEAVRYGSYSISGSPQISPSPSQSRSQDVIEIISLADQVSSDTSATQPDGLDAAEFVIAVEKCIDSDSGLVQNCTNVYLNVRKIQILNQQPTVVVAIWVDENLGNGNFTAPLIKAVYGFVYGLLMNPVCAMIVMVSEDSVRAHREERQQARFKNPEKEAMKTTAAERVAAVDPSKAMSPPAQDAKPSMAQILNNPPQRVQSLNLSWPPGLLNGHVEWANHTIIWIDALSLIEESKRRAAGLSTQEDGQVTRFVKELAGGQNLHIKFSLDEDENNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>H6QR08</accession>
    <name>H6QR08_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21353</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EHS62987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890070.1">
        <property type="nucleotide sequence ID" value="XM_003890021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QR08"/>
    <dbReference type="GeneID" id="13540922"/>
    <dbReference type="KEGG" id="pgr:PGTG_21353"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21353"/>
    <dbReference type="HOGENOM" id="CLU_2942852_0_0_1"/>
    <dbReference type="InParanoid" id="H6QR08"/>
    <dbReference type="OrthoDB" id="10512690at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003606377" evidence="1">
        <location>
            <begin position="25"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="67" mass="7113" checksum="BB7839A918654BEE" modified="2012-04-18" version="1">MFAFSTVTLVAIMVGLFQLQSGTASPTPQFVPGPYGPMPVGYGAAPIGRPVPVVQQFPGQPPMVRYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>H6QR40</accession>
    <name>H6QR40_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_21307</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EHS63015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890019.1">
        <property type="nucleotide sequence ID" value="XM_003889970.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QR40"/>
    <dbReference type="STRING" id="418459.H6QR40"/>
    <dbReference type="GeneID" id="13540947"/>
    <dbReference type="KEGG" id="pgr:PGTG_21307"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21307"/>
    <dbReference type="HOGENOM" id="CLU_1428632_0_0_1"/>
    <dbReference type="InParanoid" id="H6QR40"/>
    <dbReference type="OrthoDB" id="423559at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF22">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="4">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS63015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="190" mass="22259" checksum="3E1D8E923ADCF66C" modified="2012-04-18" version="1">MIGKTGNSSTSLPTIESLNICWFRIVLDEAHMIRNRSATRTQLIQRLDAKFFLCLTGTPLQNRLTDLQSLFQLLKMKPWSEEWIWSNFLIPNINFGSSQAIKSLNRLMDRICLRRTKDVLLNLPPKTERAVVVHLSSDWQKISHELHQTFVQSFGRLRTSADVWNSGEFFRQLTRIRQFCNHPLFAREEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRN2</accession>
    <name>H6QRN2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21629</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178284">
        <property type="protein sequence ID" value="EHS63326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889829.1">
        <property type="nucleotide sequence ID" value="XM_003889780.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRN2"/>
    <dbReference type="GeneID" id="13540739"/>
    <dbReference type="KEGG" id="pgr:PGTG_21629"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21629"/>
    <dbReference type="HOGENOM" id="CLU_083962_0_0_1"/>
    <dbReference type="InParanoid" id="H6QRN2"/>
    <dbReference type="OrthoDB" id="10584159at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="271" mass="30060" checksum="511C9ABBEBDF11F5" modified="2012-04-18" version="1">MTSNPASVATQYKAIVDELGDMGVFLSYDNLLPLVLHNSIPQGSTLRLEFDRRIDAEFALNGCQVISFEKTWHILSEAICQVRVVDSLDNLHGLPHVMAAEQDTTPREERSPSIVSHPDNVYAMASAPQHNRQIFFQCRSPNHLIGQCTAPDPARQSTAQPPRGQPPTGHFPPGFQAYYPIIAPSGAVPMYYPPCPNQLNPNSIPVQRGRMDSYRPQYGQPPNRPTAQPPEQRRLLLPRPTLPSTTLNAPQTLRATPTLRLHCLTLVPPTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRP4</accession>
    <name>H6QRP4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_21639</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="DS178284">
        <property type="protein sequence ID" value="EHS63338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889841.1">
        <property type="nucleotide sequence ID" value="XM_003889792.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRP4"/>
    <dbReference type="STRING" id="418459.H6QRP4"/>
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    <dbReference type="OrthoDB" id="2505871at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
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        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
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    </dbReference>
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    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
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            <begin position="9"/>
            <end position="99"/>
        </location>
    </feature>
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            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
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        <location>
            <begin position="119"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS63338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="163" mass="18485" checksum="87EDB361C3306359" modified="2012-04-18" version="1">MDLHTNASQFFDPGQYLIGDAAYSLTMTTIPPCKVPAANLPENVEFNYCLAKSQVRNKHAIGVLKARWSSLKEMRLHTYQASHMQAYIRWIYACIILHNMLAFLGDAWHDLASLDANEAETNDKDSEESSDEEDIPTVASENFQTKIKKKCCERHHELGTLPI</sequence>
</entry>
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        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21771</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EHS63685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889573.1">
        <property type="nucleotide sequence ID" value="XM_003889524.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSF3"/>
    <dbReference type="GeneID" id="13542706"/>
    <dbReference type="KEGG" id="pgr:PGTG_21771"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21771"/>
    <dbReference type="HOGENOM" id="CLU_168454_0_0_1"/>
    <dbReference type="InParanoid" id="H6QSF3"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="118" mass="13033" checksum="9E616675DF706077" modified="2012-04-18" version="1">MIPDQKLSHTPEFEIPPVPEAVSAPGSPHQTKTRHDVMAPTEDTGSFEEKPVVAPTEEPAKEAKPRKAVRKDSLEKQVRRSRCIFDQSHGDGSFSASAGYFLKNDGHQEGSSSKRQHK</sequence>
</entry>
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    <accession>H6QSU7</accession>
    <name>H6QSU7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21861</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178299">
        <property type="protein sequence ID" value="EHS63817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889416.1">
        <property type="nucleotide sequence ID" value="XM_003889367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSU7"/>
    <dbReference type="GeneID" id="13540578"/>
    <dbReference type="KEGG" id="pgr:PGTG_21861"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21861"/>
    <dbReference type="HOGENOM" id="CLU_2923742_0_0_1"/>
    <dbReference type="InParanoid" id="H6QSU7"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="61" mass="6222" checksum="A4EFC8ACFBAF4D56" modified="2012-04-18" version="1">MDPSPSLMTQQSFSHATNGSGILPSLGDVSLTQASPTQAPNMSQPFLAAMDDAGMDGVVNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSY2</accession>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21953</name>
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    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178302">
        <property type="protein sequence ID" value="EHS63936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889367.1">
        <property type="nucleotide sequence ID" value="XM_003889318.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSY2"/>
    <dbReference type="GeneID" id="13540692"/>
    <dbReference type="KEGG" id="pgr:PGTG_21953"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21953"/>
    <dbReference type="HOGENOM" id="CLU_2607151_0_0_1"/>
    <dbReference type="InParanoid" id="H6QSY2"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="79" mass="8883" checksum="DF078F61F51F7C37" modified="2012-04-18" version="1">MRIRIAASGLIGILTNAMPSLLDYNAVWAEIRGKSCYVSRPLGSRARRLRLVASGKYVSIEVRWKSIGEISRMPDMTWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTA1</accession>
    <name>H6QTA1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22101</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178309">
        <property type="protein sequence ID" value="EHS64046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889263.1">
        <property type="nucleotide sequence ID" value="XM_003889214.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTA1"/>
    <dbReference type="GeneID" id="13541655"/>
    <dbReference type="KEGG" id="pgr:PGTG_22101"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22101"/>
    <dbReference type="HOGENOM" id="CLU_2543695_0_0_1"/>
    <dbReference type="InParanoid" id="H6QTA1"/>
    <dbReference type="OrthoDB" id="10338536at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="83" mass="8721" checksum="14EB12E612D0129F" modified="2012-04-18" version="1">MRLGQVPLDSLYQLQLIARIGAAAAALIFQTADTLLNQPANGSMHMVQHTVDVVDALPVSEIQASAESPAPQDALVSDLVEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTM2</accession>
    <name>H6QTM2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22059</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178317">
        <property type="protein sequence ID" value="EHS64233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889136.1">
        <property type="nucleotide sequence ID" value="XM_003889087.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTM2"/>
    <dbReference type="GeneID" id="13541297"/>
    <dbReference type="KEGG" id="pgr:PGTG_22059"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22059"/>
    <dbReference type="HOGENOM" id="CLU_2813586_0_0_1"/>
    <dbReference type="InParanoid" id="H6QTM2"/>
    <dbReference type="OrthoDB" id="10396050at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="67" mass="7296" checksum="CB8379C27B367A9F" modified="2012-04-18" version="1">MALATEPNPSDGFISLLKRATIMAELDQLKIDWPASVKASGSDFTPRGPREGSDEDADKTEDRMEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTM5</accession>
    <name>H6QTM5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22062</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178317">
        <property type="protein sequence ID" value="EHS64236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889139.1">
        <property type="nucleotide sequence ID" value="XM_003889090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTM5"/>
    <dbReference type="GeneID" id="13541301"/>
    <dbReference type="KEGG" id="pgr:PGTG_22062"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22062"/>
    <dbReference type="HOGENOM" id="CLU_2997511_0_0_1"/>
    <dbReference type="InParanoid" id="H6QTM5"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="57" mass="6477" checksum="AB469CE33C3A0D39" modified="2012-04-18" version="1">MNEVCMQNLADPPKEHESDGKKTPTEVWRCCVPSATRDSFADSKVLKLRSAIMLRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUG0</accession>
    <name>H6QUG0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22439</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178339">
        <property type="protein sequence ID" value="EHS64623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888847.1">
        <property type="nucleotide sequence ID" value="XM_003888798.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUG0"/>
    <dbReference type="GeneID" id="13542434"/>
    <dbReference type="KEGG" id="pgr:PGTG_22439"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22439"/>
    <dbReference type="HOGENOM" id="CLU_1778368_0_0_1"/>
    <dbReference type="InParanoid" id="H6QUG0"/>
    <dbReference type="OrthoDB" id="10289471at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="153" mass="16799" checksum="97FAEDA7A730EF23" modified="2012-04-18" version="1">MGIGCCPAADSTQIPGRGYASGPPGMGAHRGPTNIPSKLRRNAPSSQTCHPLRFAYLYGCVQLAGFTKSARAHACWVYHGKHFYNVDAVLYDRPLCFFTHFSSRDKSGRPNPEDSYPPKVINDAKTEGKRPSYKPLRAEVRLGEAKCTAPESR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3JPS3</accession>
    <name>E3JPS3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Decapping nuclease</fullName>
            <ecNumber evidence="6">3.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_00219</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Decapping enzyme for NAD-capped RNAs: specifically hydrolyzes the nicotinamide adenine dinucleotide (NAD) cap from a subset of RNAs by removing the entire NAD moiety from the 5'-end of an NAD-capped RNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 5'-end (N(7)-methyl 5'-triphosphoguanosine)-ribonucleoside-ribonucleotide in mRNA + H2O = a (N(7)-methyl 5'-triphosphoguanosine)-nucleoside + a 5'-end phospho-ribonucleoside in mRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66928"/>
            <dbReference type="Rhea" id="RHEA-COMP:15692"/>
            <dbReference type="Rhea" id="RHEA-COMP:17313"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:172876"/>
            <dbReference type="ChEBI" id="CHEBI:172877"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:66929"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 5'-end NAD(+)-phospho-ribonucleoside in mRNA + H2O = a 5'-end phospho-ribonucleoside in mRNA + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:60880"/>
            <dbReference type="Rhea" id="RHEA-COMP:15692"/>
            <dbReference type="Rhea" id="RHEA-COMP:15698"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:144029"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:60881"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 5'-end triphospho-ribonucleoside in mRNA + H2O = a 5'-end phospho-ribonucleoside in mRNA + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:78683"/>
            <dbReference type="Rhea" id="RHEA-COMP:15692"/>
            <dbReference type="Rhea" id="RHEA-COMP:17164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:167618"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:78684"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the DXO/Dom3Z family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.-" evidence="6"/>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74263.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307269.2">
        <property type="nucleotide sequence ID" value="XM_003307221.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JPS3"/>
    <dbReference type="STRING" id="418459.E3JPS3"/>
    <dbReference type="GeneID" id="10527639"/>
    <dbReference type="KEGG" id="pgr:PGTG_00219"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00219"/>
    <dbReference type="eggNOG" id="KOG1982">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_460138_0_0_1"/>
    <dbReference type="InParanoid" id="E3JPS3"/>
    <dbReference type="OrthoDB" id="2498214at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034353">
        <property type="term" value="F:mRNA 5'-diphosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110155">
        <property type="term" value="P:NAD-cap decapping"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013961">
        <property type="entry name" value="RAI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039039">
        <property type="entry name" value="RAI1-like_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12395:SF9">
        <property type="entry name" value="DECAPPING AND EXORIBONUCLEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12395">
        <property type="entry name" value="DOM-3 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08652">
        <property type="entry name" value="RAI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RAI1-like" evidence="8">
        <location>
            <begin position="87"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00044676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU367113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP74263.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="609" mass="69013" checksum="9FCB8F087C0D2597" modified="2012-04-18" version="2">MRIDFNLLSCDPLTPPSSPKKTAKKKAREKKQAASPSHDHHQQTKKLARVVLEAIPISNPTRFILPKPVYNNEFPHQSARPFKQNYQTPTPITSFSYDQHGKIHCGAHDQFISLRPFLDNVDHTGFDLNTGIDEAVYVEDAVDRGIDGLLLSLADFLDRSQPADRLTKSREILSTELITWRSALAKLALTAYENRQIDQNASGVWTKRVMLIDGTIYLEDEPEQIEDLVEDLDELPAGYVEEDGIIRRSDEWAAHIYHGYSYESLMTNTEFQSVNTNSQWISVVSSTLNGIPIILGGEVDCVTLESFQKVHTPNHHHHHQESVDHNQHPLPKFVEPDQTIELKTSIVPSTIHELCNLHRYKMIKYWLQSFLMGTPKVHVGFRTREGILHSSKTYQTNEIPGLVKREAQPYCRWSSDVCLETGTKIIRFLKNKLARGPVNRKASRRADPSAREAWINTTTTTQGYSNRSQFFNDRLDAIVAEEYARQVANTRPQEDPCRLSDICKAQVMDLGRWPVYRLVFKPYTAPDLSPTQSSHAPLPTGIPAFIQLEELSFKEVSESILCHKPKTLFNLGYPSTTSTTAGSNDQDFNCHDRDLDSTIGPDAGRFIAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQ74</accession>
    <name>E3JQ74_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00320</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307370.1">
        <property type="nucleotide sequence ID" value="XM_003307322.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQ74"/>
    <dbReference type="GeneID" id="10546903"/>
    <dbReference type="KEGG" id="pgr:PGTG_00320"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00320"/>
    <dbReference type="HOGENOM" id="CLU_915675_0_0_1"/>
    <dbReference type="InParanoid" id="E3JQ74"/>
    <dbReference type="OrthoDB" id="2506296at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP74364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="304" mass="35333" checksum="CA929D7479FEB6DA" modified="2011-01-11" version="1">MHIFSSNPRILIIRLEAAWFTRSSDIFISIPATVLWAFLHQNPVGVSERPEFRAVCSAIHEPYPKVFIKITHHGQSHTSRIYSQLPSPYRPSIQIMRIHNHTKVNNQEFTGKAVSRDATDLPQNEQQPSDHTDLRTSKTMKTSTRFWDNKSLLCVRERNDDDLKTSTASEEKAMDFNPAVDAMNAFRIATLIVFGLTVLTIGGLSKRWELDGWEDWRIFIQSGHLGKLLIPENWSRSASQAIPDRLRPTPVPLSEQELLDELELISSWSWKKRVDREWEVERNRREAERLQWEAQRKALGKKIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQR3</accession>
    <name>E3JQR3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_00469</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307519.1">
        <property type="nucleotide sequence ID" value="XM_003307471.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQR3"/>
    <dbReference type="STRING" id="418459.E3JQR3"/>
    <dbReference type="GeneID" id="10527670"/>
    <dbReference type="KEGG" id="pgr:PGTG_00469"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00469"/>
    <dbReference type="eggNOG" id="KOG3069">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1422039_0_0_1"/>
    <dbReference type="InParanoid" id="E3JQR3"/>
    <dbReference type="OrthoDB" id="10260614at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010945">
        <property type="term" value="F:coenzyme A diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045121">
        <property type="entry name" value="CoAse"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992">
        <property type="entry name" value="NUDIX HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12992:SF45">
        <property type="entry name" value="NUDIX HYDROLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP74513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="191" mass="21442" checksum="79B4171ACB1AA492" modified="2011-01-11" version="1">MSAITLNRHKVRKLTTLAPFLSRGNLIVTPVVFFIADQTLMPRLNAKEVDVLFSHPLENFLTGPFSRSYQIPWFSTNVPYRLFEFPSKHSPIVGFTADVLIEVAVLGFGREPDFERKAAGQLPMTEIITIALREAPEFQSDDQAHQVASHDILEDGPRSPLARLRHALQLPSNLHVSETFSSLRKLIGAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQT7</accession>
    <name>E3JQT7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ubiquitin 3 binding protein But2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_00265</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74309.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307315.2">
        <property type="nucleotide sequence ID" value="XM_003307267.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQT7"/>
    <dbReference type="GeneID" id="10527974"/>
    <dbReference type="KEGG" id="pgr:PGTG_00265"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00265"/>
    <dbReference type="HOGENOM" id="CLU_1504130_0_0_1"/>
    <dbReference type="InParanoid" id="E3JQT7"/>
    <dbReference type="OrthoDB" id="2504656at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171462" description="Ubiquitin 3 binding protein But2 C-terminal domain-containing protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP74309.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="199" mass="21333" checksum="7671C663EAC52012" modified="2012-04-18" version="2">MRNFTKLFLSLSHILLAFCREDSKPLNANSSSPGEDQGSNLPTSPYASIAKTAGPSGILIQPKGGAVFKNHVGAVGNVEVIYKGVSDGTNDVGARTCTIDLHLIPVSNQSQTGSINLSSESVIHLSYGLRPHDSGSDQPIWANFVPPPGACGDFHLVVYERQLYKDTVIHFQSAAPIIRFECANMVAPKIYPPNSISEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3JSC0</accession>
    <name>E3JSC0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01438</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP74845.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319264.2">
        <property type="nucleotide sequence ID" value="XM_003319216.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JSC0"/>
    <dbReference type="GeneID" id="10546961"/>
    <dbReference type="KEGG" id="pgr:PGTG_01438"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01438"/>
    <dbReference type="HOGENOM" id="CLU_1540844_0_0_1"/>
    <dbReference type="InParanoid" id="E3JSC0"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP74845.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="174" mass="19946" checksum="8DFFD075C6664691" modified="2012-04-18" version="2">MSAAYRRATHIPPGGHGSTPRPRRPPGFKAANLTSADRKVWLGRRRSVRPSRYRAGCRRDAPVVDHINQLGIFLFGHISNSKPEDDMFAAVVVSTTIIAVAHLYFTRQETPRQEGHRREAEQKRQEAERKRQEAERKRQEAHAAMMKIQEEILLRVRELAKAVAELKITHDSRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>E3JT76</accession>
    <name>E3JT76_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Programmed cell death protein 2 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_01948</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75355.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319774.2">
        <property type="nucleotide sequence ID" value="XM_003319726.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JT76"/>
    <dbReference type="FunCoup" id="E3JT76">
        <property type="interactions" value="443"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JT76"/>
    <dbReference type="GeneID" id="10542902"/>
    <dbReference type="KEGG" id="pgr:PGTG_01948"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01948"/>
    <dbReference type="eggNOG" id="KOG2061">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_525931_0_0_1"/>
    <dbReference type="InParanoid" id="E3JT76"/>
    <dbReference type="OrthoDB" id="2499641at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007320">
        <property type="entry name" value="PDCD2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47524">
        <property type="entry name" value="20S RRNA ACCUMULATION PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47524:SF1">
        <property type="entry name" value="20S RRNA ACCUMULATION PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04194">
        <property type="entry name" value="PDCD2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Programmed cell death protein 2 C-terminal" evidence="2">
        <location>
            <begin position="310"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="171"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="284"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP75355.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="416" mass="46019" checksum="D55A1DBBB8FB2219" modified="2012-04-18" version="2">MNHLNARQDPSDSDSDSSQLAQYTLGFDDGKINKLSEVEQYSISRIGGIPVLPPFENTLVPEKDLECLICQKPIPMLVQVYCPSDQNPYQDRVIYLFGCTEQKCQTQGSSKCVRGFKSWKFNEEYYRQLSAQQTRSSLPQTALNNQSDLGDNPFKMNTHNLFGQSFDNPFSAPPAPTQDPPPAVAESSLLENPFSNLSLGEKESATTTTSSAQKHNAKLVARLPALCTPPHYLATDYEVKPKLSSSTTFKEFLDDQTQPPHPSSVTSSKKSEQNGHSSKKKNASSGPPTTPSGPSGAQESYEVQAVKGVDPAFLDFQDRINWNDQSTQIIRYNPNGRALPVHSTKPTPSIPALQCGHQSRFELQLMPAVLRLFSCPIDWTTVWINDCVQDCCRPASDGGCREGWSEVQCYVQSVLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>E3JVA6</accession>
    <name>E3JVA6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_01312</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320400.1">
        <property type="nucleotide sequence ID" value="XM_003320352.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JVA6"/>
    <dbReference type="STRING" id="418459.E3JVA6"/>
    <dbReference type="GeneID" id="10533061"/>
    <dbReference type="KEGG" id="pgr:PGTG_01312"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01312"/>
    <dbReference type="eggNOG" id="KOG0017">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0670">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_5_5_1"/>
    <dbReference type="InParanoid" id="E3JVA6"/>
    <dbReference type="OMA" id="FKAVHIY"/>
    <dbReference type="OrthoDB" id="9332038at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045292">
        <property type="term" value="P:mRNA cis splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14135">
        <property type="entry name" value="STKc_PRP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000078">
        <property type="entry name" value="Serine/threonine-protein kinase PRP4 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044092">
        <property type="entry name" value="STKc_PRP4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF103">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE PRP4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="624"/>
            <end position="952"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="350"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="411"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="474"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="12"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="38"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="94"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="134"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="150"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="239"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="299"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="350"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="373"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="417"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="445"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="474"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="493"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="538"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="661"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP75981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="955" mass="107674" checksum="1C17C6EBF73903B4" modified="2011-01-11" version="1">MIETPRKRTFGSIHSSSSPNSSSTRQRDQDNPWFRDPSQQDKKEYKARKNEHTSSPRSRPSHHRVRSPPPSGGDRSNQQSHDEKERVPTNGSRPSDENRDSTIRGLKEPGPAKKSTPVPSDDTVEDGEIAEGAPSSNHSPSPSRRSQSVKSDRDRHRDRARESDRERLRDLDRPSIRHRQHDSDRDRPRDRPRERRVDSREREYVRDSHRERERRKDRDRDHGRFAPPPSYSDHHRRSPDRDRDHPRRRFAQEERSSRHHREDYHRRNSERRSTDSTQLTGDHLRRPSTSHNHSTKQDDNDEDKVDDNDEATGVAEPTPQLTEDEILEQARRKRAEILAKHKQLCQTVNPSDVFTMTTDPSLRVVHNPPTDPPKSSTSSATSNPIPTKANHLPLLLDPTSKVADLNSPAARMRLGSDTPQPTRIGSEQSSDSAAGSPEPVAETNFDLTQDPNNQVSTTTIDDEESVAANHPLDQQTITGVGHQSISAADYNPDDDRRIDNERQNEKHVNGHRLGEQPPPPDTQPGGNLSEREGANGMDVDENGDDDGGDDDDDDDMFAIDSTEKKAKKKKATNGNNPKNPLNPSAPFLPVINRAGTDAEVVDNFDDADGYYRVILGELLDNGRYHVHANLGKGMFASVVRAKDMSSAKDGIQGSGSDVAIKVVRSQESMFKSSQKEAGILKKLQEADPAGKYHIIRLERTFEHRGHLCLVFEAMSMNLREVVKRFGKDVGINLRAVRAYAHQMFLALSLMKKCNIMHADLKPDNILVSETKSVLKVCDLGSASDVTENEITPYLVSRFYRAPEIILGLPYDCSIDIWSIGCTLYELYTGKILFPGRSNNHLLLLIMELKGRFNSKLLKKAKFSNVHFDDENGNFLSVEKNRITGTDIVKSINIPNKPTQGLQSRLMPNHVTKKMQDADVKLLSAFVDLLEKILTLEPSRRISPKEALNHPFIRGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3JVB4</accession>
    <name>E3JVB4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bms1-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_01320</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TRAFAC class translation factor GTPase superfamily. Bms1-like GTPase family. TSR1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75989.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320408.2">
        <property type="nucleotide sequence ID" value="XM_003320360.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JVB4"/>
    <dbReference type="FunCoup" id="E3JVB4">
        <property type="interactions" value="478"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JVB4"/>
    <dbReference type="GeneID" id="10533231"/>
    <dbReference type="KEGG" id="pgr:PGTG_01320"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01320"/>
    <dbReference type="eggNOG" id="KOG1980">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009858_1_0_1"/>
    <dbReference type="InParanoid" id="E3JVB4"/>
    <dbReference type="OrthoDB" id="2501453at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034511">
        <property type="term" value="F:U3 snoRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000479">
        <property type="term" value="P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012948">
        <property type="entry name" value="AARP2CN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039761">
        <property type="entry name" value="Bms1/Tsr1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007034">
        <property type="entry name" value="BMS1_TSR1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030387">
        <property type="entry name" value="G_Bms1/Tsr1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858:SF1">
        <property type="entry name" value="PRE-RRNA-PROCESSING PROTEIN TSR1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12858">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08142">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04950">
        <property type="entry name" value="RIBIOP_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22298">
        <property type="entry name" value="Tsr1_G-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00785">
        <property type="entry name" value="AARP2CN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01362">
        <property type="entry name" value="DUF663"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51714">
        <property type="entry name" value="G_BMS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Bms1-type G" evidence="6">
        <location>
            <begin position="115"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="67"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="390"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="500"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="422"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="457"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="500"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="513"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP75989.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="830" mass="92754" checksum="52780A9B340F413A" modified="2012-04-18" version="2">MTATKTAQQQHHHRSTLSQTNKPFKARHATKGSLKQVAKGWISTLGFNEQGFNILMIVLLSAPGRLEKDSSTNKSKHASQTGQKANRKNQAKQLQAHKAALQAGCQKVFSGPNPAPRVIAILALSEDVDPLVDVLNPIRSASNLTHPIDPSSRTISFTWPTSSRSSPSLLQFLLIPHRTPLGQVLSTVSASDFLITSLSAVEEVTAWGESTLRAIQALGGPTLGVLGLVSHLESLNGTRETQKTRESLTSFLRYFFPESLLLNRLVSVDRPEEILVAVRSILAKLPNRASNGLPLGWREGRARLVAEKIDWEEGTLKVTGHVRGGRFSANRLVHLPFFGDFRVSMMTMAEDGKVISVRTNEEADDLVSENCPSVGDELMAEQTWPTEEEIASAPAHQQKTAGNKKARRVPVGTSEYQAAWIVDEDGEGLEDDDDEEEEEEDGDIEGDIEFDKSPDQLEQDDDDEDEMEDLKVDNQAGSSKDVHFMDLSDEAEQADLAQYRADRQRERDRAAKEDEEFPDEIDTPLDIPARERFARYRGMKSLRTSEWDPYENLPIDYSKIFAFQGWKSMGRKLAHKANEQEAGAGPGMYITLHIEQFPQSGFEALVSSPQQTLVAFSLLKHEHKYSVMHFTTQRNTDRRSERATTYTSLSAICDPGKMNIGSVYMPVTFGSTTPVLVFKQEEDLDLPISFVGNGTLIGSEPQRIIAKRIILTGHPYKVHKKTATIRYMFFNREDIEYFKPIELKTKKGKVGHIKEPLGTHGYFKAKFDGMIDQMDTICLSLYKRCFPKWSTTKLINLDHHLSAVFFNSNHINHCSTGHPSLTSGTMEIDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>E3JVE6</accession>
    <name>E3JVE6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_01352</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP76021.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320440.2">
        <property type="nucleotide sequence ID" value="XM_003320392.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JVE6"/>
    <dbReference type="STRING" id="418459.E3JVE6"/>
    <dbReference type="GeneID" id="10533263"/>
    <dbReference type="KEGG" id="pgr:PGTG_01352"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01352"/>
    <dbReference type="eggNOG" id="KOG0586">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_504458_0_0_1"/>
    <dbReference type="InParanoid" id="E3JVE6"/>
    <dbReference type="OrthoDB" id="289250at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF572">
        <property type="entry name" value="FATTY ACYL-COA SYNTHETASE AND RNA PROCESSING-ASSOCIATED KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 12" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="144"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="73"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="411"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="461"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="9"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="73"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="411"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="464"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP76021.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="540" mass="60954" checksum="C5108A72F5C2B004" modified="2012-04-18" version="2">MIKPHQERTTSTSSNSSNSSTSTDLLQLSSSASSTSSSSSSSSSSSSSESQLKSITFSTARLLSYNQAQPINSAIIPNSPSDKQHPQSTPPALNYNQNYSLSQPQLSTTRQQQQQQQQPAECSTSQPQDPLIGTTIISPNRTHYYLNRILGQGSFSKVIQAHPLNQPNHSVALKLIRITTPTNSIPNNTPLQHHHLITQDQMPTDETENIRMSTLREIEILKSIDHPTIIKLIETFTITNDSTNLIKKNSSLQIIVLEELRGGELFELVSRSKSTLRPLIICRLMAELCISIHWLHQHDIVHRDLKLENILLVREIKNIQEEETEMLTNPNVYLKVTDFGLSVKLSKSEDPKHSTESGKKFLQSRCGSEEYAAPEIILGREYDGTKTDGWALGVIGYSLVMGFLPFKLPQRKEPGAEEDSQRTSLAHSNELPEDDSEQAYLKKRKQGLLKIIKSEYRWKPNHSTTHHHHHQHQQPHHHPTQNQIVADDHQLVQGQIQLVDRLLVRDPLKRSNDLLYGFRDPAWLGQFNWLKDCLLTSSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3JVK2</accession>
    <name>E3JVK2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_02518</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76077.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320496.2">
        <property type="nucleotide sequence ID" value="XM_003320448.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JVK2"/>
    <dbReference type="STRING" id="418459.E3JVK2"/>
    <dbReference type="GeneID" id="10533481"/>
    <dbReference type="KEGG" id="pgr:PGTG_02518"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02518"/>
    <dbReference type="eggNOG" id="KOG2273">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_329316_0_0_1"/>
    <dbReference type="InParanoid" id="E3JVK2"/>
    <dbReference type="OrthoDB" id="2414662at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047168">
        <property type="entry name" value="LEC1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024554">
        <property type="entry name" value="LEC1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024555">
        <property type="entry name" value="PX-associated"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47185">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN YPR097W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47185:SF1">
        <property type="entry name" value="PX DOMAIN-CONTAINING PROTEIN YPR097W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12825">
        <property type="entry name" value="DUF3818"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12828">
        <property type="entry name" value="PXB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PX-associated" evidence="3">
        <location>
            <begin position="3"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="PX" evidence="2">
        <location>
            <begin position="370"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="816"/>
            <end position="836"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP76077.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="872" mass="99769" checksum="C8258AB62B17DA11" modified="2012-04-18" version="2">MPEQSFTAFDLHSLNKILASFELQREWKELSRVNSLKAIGYPFDQSLQQPIQQPTPVLKKQPSRISTVLSFFGSPSAPPSARSVSEAESQLGSNLRERQAVRDRAKGDVDQLPLFRLMIQRVWGAFPGLIDLPIETWLGIERFILDFNSRNFSTSRERRQFTKRAVISIGFTKLLSSYITSIISFQNQLSLPTRPSQEDLDLVSQLPQLISTFGSRIKFVERKNDEFLVLYRKKTGRPKLPIDSSAGENLSLNVGISVIGWSALCDLLKTIDPSTGHHDRQTSISLLRRVFHIQSSQAEKLYQVLEAAHPNPSSPDDEIKNELDKFLADERGFQTDWISTGKRVSEAKEGYKNFLTKIVHHDGEIDKMYKLITEHERLETVLALDPMYEQAQTWTIMWIAYMLHFVFITGPDGDEVCELFIKIDKLVPYELIKQGLKIINPTLAIRTTVALILGQPFGSLSLFQRILDSIVRHEIRCYDKKIETVVQSSYPPVTHPSELSRMKSLAAIKKFVYLPEEEKRKTRAEFDQSDEDMIILILRWSKDFDEVELQSVQESEKIFNNSSGSLADANLFHDLIILLRLVSLKRDREQIIEMITEPNNPIIRTIKQILEIYYPTIYKVALASDLSKKFGQTEAFLKDLVQLLVRRKEEEVSVDKLVKLLDQHKQSYWSFLNDLVRCENLCDPMKEWIQSCFDLVKSGLNESEAASGSGRFGIELEELRSAAPIEALIAESRSLSTYNRLIKMIDNIQYRLVLCSSPFTLETHFSSQAFHILTELDQLPFARHLDQSARDARPPLPFGWAWWIDEQKVCGKGGDRVNDDGAEVQGSPIKDDQPLGPSRLVVNSDLHELKKRASVYLELLMKSVHLFDRPTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>E3JVZ0</accession>
    <name>E3JVZ0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02656</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JVZ0"/>
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    <dbReference type="KEGG" id="pgr:PGTG_02656"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02656"/>
    <dbReference type="HOGENOM" id="CLU_1876463_0_0_1"/>
    <dbReference type="InParanoid" id="E3JVZ0"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="31"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="136" mass="14781" checksum="68A02093E9773029" modified="2011-01-11" version="1">MASVTVTIEGTTAGSGSFLLRKRASEGRANKVTFTTPRLSTKVGRELSKSPLVARGEGGGQGLNLGKSKTRLREGWRRQYTTTTPSCKANQQTYHKTCLKGVHVPARKRTPFPPSNAAAQPRKLKNPCEENSSVRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3JW92</accession>
    <name>E3JW92_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_02758</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76317.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320736.2">
        <property type="nucleotide sequence ID" value="XM_003320688.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JW92"/>
    <dbReference type="GeneID" id="10534209"/>
    <dbReference type="KEGG" id="pgr:PGTG_02758"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02758"/>
    <dbReference type="HOGENOM" id="CLU_1611593_0_0_1"/>
    <dbReference type="InParanoid" id="E3JW92"/>
    <dbReference type="OrthoDB" id="10352185at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP76317.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="165" mass="18948" checksum="9A5DA95B0E00479B" modified="2012-04-18" version="2">MPRGYAQMRASAMNQVFELCQHLIEQAKVQYRDMVWGQLQINPAEHLGPLPSQPEFNVALKPEFNADWVRMAHIRLHMVHHKYALPESSLSEWSTLDQELHELAQKDQKYQIAHAQSILIRDHELFNGINTLASIPHANLRLPTADEISMRLELIAQGVNDAPWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWU5</accession>
    <name>E3JWU5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_02961</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76520.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320939.2">
        <property type="nucleotide sequence ID" value="XM_003320891.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JWU5"/>
    <dbReference type="GeneID" id="10528293"/>
    <dbReference type="KEGG" id="pgr:PGTG_02961"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02961"/>
    <dbReference type="HOGENOM" id="CLU_3015255_0_0_1"/>
    <dbReference type="InParanoid" id="E3JWU5"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP76520.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="56" mass="6091" checksum="3BFAF40EEB485CEA" modified="2012-04-18" version="2">MYSSAVRTPIPTINVKYPPTSSPVSDVISADPPNQYPVVSVNRAENEIPSCTICQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3JX24</accession>
    <name>E3JX24_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Oligopeptide transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_02060</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
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        <property type="protein sequence ID" value="EFP76599.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003320970.2"/>
    </dbReference>
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    <dbReference type="GeneID" id="10528456"/>
    <dbReference type="KEGG" id="pgr:PGTG_02060"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02060"/>
    <dbReference type="eggNOG" id="KOG2262">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004965_3_1_1"/>
    <dbReference type="InParanoid" id="E3JX24"/>
    <dbReference type="OrthoDB" id="9986677at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004648">
        <property type="entry name" value="Oligpept_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22601">
        <property type="entry name" value="ISP4 LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="222"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="253"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="291"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="335"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="402"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="434"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="481"/>
            <end position="502"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP76599.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="537" mass="59240" checksum="042F7B4AF9CC9381" modified="2012-04-18" version="2">MLYFRKASIYIALQVMFFSDILTFFSNLSETLPSISLLKSLFSVGEESKDQVKFFKKAFLTVIVYEIFPTYITPALQAVNVFCLTLPKDPIVTSIFGGAQPFQGMGIFSVSGDWSMVGGSGYNQHFPFLSVGLFTSEGKPYPYREAVNQDGTANEEYLEKSGLPFFTGTYYIVQILLTTSLTSSIAHAVLYNYRIFGAMFKKSKRSADIDPHRLVCQKYKDFPLWGFILLALGAIGLAFGMSAISNSGLSAPALIVALIVSFILTLGTGFIFALTGFVVRLSPGIQMLGGLLFPGNVFASMWFTVYGGTSAYQGLNILKNMKYGQYIHLPPRLVVYSQLIGCTVGSLTTLLVARAIVSHEREVLLSPHGNGIFSGAEIAAFQARAVSWGLFSRHLFLSGQRYSAVSWGMLIGFALPVPFFVAHKIWPRLKFNQVIVPLFLGIIPGLYNMAYAGELARMVIGLTSQLWARRYRAQWFNKYNYILSAALDGGTEVAVFVLAMAFQGGGGRQIKFPTYFLNPPSSTPRDYCWVDPNSRAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXA2</accession>
    <name>E3JXA2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76677.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003321048.2"/>
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    <dbReference type="AlphaFoldDB" id="E3JXA2"/>
    <dbReference type="GeneID" id="10528616"/>
    <dbReference type="KEGG" id="pgr:PGTG_02138"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02138"/>
    <dbReference type="HOGENOM" id="CLU_2347735_0_0_1"/>
    <dbReference type="InParanoid" id="E3JXA2"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172210" description="Secreted protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76677.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="97" mass="10916" checksum="777DEEE5E07CF4DD" modified="2012-04-18" version="2">MSGDGVLMVLLACRVSFAASDEEFVPPTTLNNNHHHHKETHYLDGNTLRIDLNKCGWTPKPLAQTTHLATITIKRNLILSKMGKPNQKIQKPIDQFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXD5</accession>
    <name>E3JXD5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02171</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76710.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321129.2">
        <property type="nucleotide sequence ID" value="XM_003321081.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXD5"/>
    <dbReference type="GeneID" id="10528730"/>
    <dbReference type="KEGG" id="pgr:PGTG_02171"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02171"/>
    <dbReference type="HOGENOM" id="CLU_336531_0_0_1"/>
    <dbReference type="InParanoid" id="E3JXD5"/>
    <dbReference type="OrthoDB" id="10301796at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="279"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="776"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="186"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="295"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="779"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="819"/>
            <end position="859"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="860"/>
            <end position="882"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="890"/>
            <end position="904"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76710.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="942" mass="102938" checksum="2D01F918F303B632" modified="2012-04-18" version="2">MTGQPVFGINTSFAGGRTYCEGNTNTYGHQTSNQQLSLPLTSGSDISYNQEPNHRSAHTSYNQGQNYRGATSTPHNQQYHTPDGLNSYQNSIQNPKTSYTHQHITQNSPPALQGRRIISGPNISQIQLQGQATTDRHLLNVQLQGQATTDRHLLNGQPMLTTQATSHSRAQAPGSHNNQIPRDTLPHLPSPLHSTPRFQHPCGNTTGQSQPNSSPIQVRTVLHPHTNVHPSGLNNQTRLPNSASTTHQQEDCVPPPRNYRLSVSQPQSVLVHQATIANRVPPPQTNTLAPLQARSHQPSQSADPSSASVHQTTISNRAPHSQLDSSATSQAQSHQPGQSANPSSASVHHTTISNRAPRSQLDSSATSQAQSHQPGQSAKSSHPSLDNDINLAGIMSGSMDSGKEVVRSLTEDEMMRFKDMNLSILRQIASDKPPRSRMTAEMKSELDDLYYEFQRELHKLSIKHQVGPHLCFAHLGQSKKVKGGSTWNNFQQYDPEALALFDEFGKDVGGDKVAELWKTKDDATKKLYKDIDFLISLTEENADAIAAAQDNVYSTNGSRLRSKTMVSSSHVSQKKTMTMVSNWVRKTEADLKSMAFFHQVEGFFVVASRHPKSTIFEKGGSALGYNFLEMMADKEEKDTAGHFHTWVAAKAIQIINGIRVDPSKSMKRKAITSTNDKTNVANEFDMGTVAKNVHAIREKLKEMIQSASGNELCCAWPATDSVNKLKELKLKLHVKNNDWNIVPKDLFQPLSRLHEGPAKGILACLNTKRILITYHGNEDSEDETTDSTETESDSDDETDTTEDESDSTKSSVEVDNPPPKKRAQKNSKTGSSKKKSGSSKKKSGSSKKKSVTSKKKPVTKSKSAVNKKSTSSKQKRTSAASTNQISDTVTTQNSNTLTTQNSDTLDPRSSLAGISGAQEGGTGLVGDSQIQSSIDPLLETLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3JY27</accession>
    <name>E3JY27_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_02413</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321371.1">
        <property type="nucleotide sequence ID" value="XM_003321323.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JY27"/>
    <dbReference type="GeneID" id="10529219"/>
    <dbReference type="KEGG" id="pgr:PGTG_02413"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02413"/>
    <dbReference type="HOGENOM" id="CLU_2198273_0_0_1"/>
    <dbReference type="InParanoid" id="E3JY27"/>
    <dbReference type="OrthoDB" id="10481809at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP76952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="108" mass="11917" checksum="15F345E1330CBCFC" modified="2011-01-11" version="1">MAKTRWIVAHWSWVAAGRSTRKSRELQCVCVFIPSRLGCPSVRPAKVEGARRLKSLTGLAVRGLYYFWQVLFGSDQAFPNQSQTPSPAAALVISGFPVSQSTHLRPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZK0</accession>
    <name>E3JZK0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_03431</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321894.1">
        <property type="nucleotide sequence ID" value="XM_003321846.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZK0"/>
    <dbReference type="GeneID" id="10538353"/>
    <dbReference type="KEGG" id="pgr:PGTG_03431"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03431"/>
    <dbReference type="HOGENOM" id="CLU_086121_0_0_1"/>
    <dbReference type="InParanoid" id="E3JZK0"/>
    <dbReference type="OMA" id="CIWASGN"/>
    <dbReference type="OrthoDB" id="2496053at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173095" description="Secreted protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP77475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="277" mass="30071" checksum="8B726B18CD46B877" modified="2011-01-11" version="1">MHITSQSILFAIGLLGFVVADGLLPQSTPMTDDHSSSNPTPSTTKPKKPKHSQCYNYFLNKDKCVFAAKKKSERCPVDSKDQCGHAIPTDMPIVFKSKHKHSSQSTMGLEIGPNGINTFASKSEEDEASTEFSAGVPSKKLVRRYDSELDSFPIAGGDGICGNYKDTAPGVCLWASAADVPGSLSGGWINGTFTKPCGRQVFIQRKGFPEHTVYAPVVDGCTFNATTEEPGCFRIAFTQATFNELNPTQKEIDDKIIHELVWDFDNALGTKPENAAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZT3</accession>
    <name>E3JZT3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03514</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003321929.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZT3"/>
    <dbReference type="GeneID" id="10538597"/>
    <dbReference type="KEGG" id="pgr:PGTG_03514"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03514"/>
    <dbReference type="HOGENOM" id="CLU_486732_0_0_1"/>
    <dbReference type="InParanoid" id="E3JZT3"/>
    <dbReference type="OrthoDB" id="2502602at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="74"/>
            <end position="105"/>
        </location>
    </feature>
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            <begin position="218"/>
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        </location>
    </feature>
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        <location>
            <begin position="363"/>
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        </location>
    </feature>
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        <location>
            <begin position="430"/>
            <end position="486"/>
        </location>
    </feature>
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        <location>
            <begin position="83"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="314"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="469"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="559" mass="61150" checksum="E0BF8FCD218A0DA5" modified="2011-01-11" version="1">MTVSRPQSSLSNHSDDQRLHFHRQLMGRANSSTATNQLELHQPLLPNRQSNLGLNTTSCSFIDPLRNSTTDLSPYHHAMFNHPSSTSTRPSSRSSNYSATSPLPIPPIAIGSPTASLSSRHSSSVRGDLYNAASLFPLPSTSFRPATAGSGQFFDPDVEAERVRLAKLEQRRSLALSRPNMNTARNSSEGSIRSVHSFRSVGPSHSKVLGPPGRFGTNAGSVYRPSPLRSASTPALATMEAEAEKKKQRKEMKEEYQKMRAARIEEEKRMSQQAGEKYPSQDSPGWFASRFKRSKELPSTRRIAGHGIDDRRSTSQSSATESSAPSSSPLDDGSHSTVPTSVEEDYQELGQRPKNEDLLEDLSDHMSSFDSPPIDPFRKSMLPGQVPPTPTKRPIATGQTPKTIGRTLPSRMNIIKLNKLLESFKIGGATAPPTGLNDDDSTTTPISITQKEDKRKPKISVVESRSPIPNNHPSSTLNNNNNPSLANEAQSGWFGSIKSIRHKAARLSRVDTRNSTIDPLNAGKRRKSIGGLFFNSSVVNNQAELIPPVPKLPVDLVHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3K0L0</accession>
    <name>E3K0L0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Bromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_03791</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003322206.1"/>
    </dbReference>
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    <dbReference type="STRING" id="418459.E3K0L0"/>
    <dbReference type="GeneID" id="10540819"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03791"/>
    <dbReference type="HOGENOM" id="CLU_735963_0_0_1"/>
    <dbReference type="InParanoid" id="E3K0L0"/>
    <dbReference type="OrthoDB" id="2505452at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016586">
        <property type="term" value="C:RSC-type complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Bromodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001487">
        <property type="entry name" value="Bromodomain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037382">
        <property type="entry name" value="Rsc/polybromo"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16062:SF19">
        <property type="entry name" value="PROTEIN POLYBROMO-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16062">
        <property type="entry name" value="SWI/SNF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00439">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00503">
        <property type="entry name" value="BROMODOMAIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50014">
        <property type="entry name" value="BROMODOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 8" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="3" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Bromo" evidence="10">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="59"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="117"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP77835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="376" mass="43119" checksum="2A03A67437753DDF" modified="2011-01-11" version="1">MDLPSAVDYPDYYQFIKHPIALNLIKSQLDSEHNPYLSFDKLLSDLKLVFSNAKKYNVGDCDHKNEAEAPEPKRKKSKLELHPSQRTHPIPTPSTTPANGIKPTPPSTFVIPPTPVNPVSHNQRSAQTQPQPPKHLRSLAQLNQVALPLEEGDPKSDHFKVCGEYPTDLGDCPEGSPEFRLTRKQRRNCNRNQTKERQRLEDLRNHADLSNFSVHRLCSNQPLRLVQKNEIITKNKRVGGIVRFTKFSDIDPLLLHQFEALSQHLLEQSRYLNPNGNNGNKLGGTMFNAGWRKAYTHNEILGISASVPKISGHEERYLELQEEMKTQEKFLATRFAHLSRLLYDTLRIQHKELKLLFRATRLLFCISSQFHPQQLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2A1</accession>
    <name>E3K2A1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04426</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78470.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322889.2">
        <property type="nucleotide sequence ID" value="XM_003322841.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2A1"/>
    <dbReference type="GeneID" id="10536150"/>
    <dbReference type="KEGG" id="pgr:PGTG_04426"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04426"/>
    <dbReference type="HOGENOM" id="CLU_532244_0_0_1"/>
    <dbReference type="InParanoid" id="E3K2A1"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="120"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78470.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="413" mass="43964" checksum="1F95EC0D245DDA5C" modified="2012-04-18" version="2">MEFAGVAGAIATGQAVHNVATAREGAAALAVSNTANDLGKATATVDAMGPAKLQRTTSSPGDLELFKSDIHRSDEIEFQDAHSEFPGHKKTLSLDNSLTQKPKTLESQGSAFEYAVNKNEDLVKPPKQLSTGHDTLDPNGASTSHSTQQKPNEVFSPPTETTPNPAPPQPELNKGDVQNPHAPGQPEISNAPKALDQSADPNRLQGLDSNPAKEYGKSKSALVTGEPTHPADFQRFNSLKPTNQYTPVNKVELANSAKALEKTPKHVGVPLNPKAATVKPSTRFQKFISLFVRKRTSKINGVEVAKLEVPRETPKSLTPGSAPRPPSTEQVPKPSTETPNDLSAAPSNHLSTETPKPVNPADPKAVSPEAEKAAPRTWKQYFSDKYKEFKSQIVAAKPATETASTASRAAPGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3Q4</accession>
    <name>E3K3Q4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_04662</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78706.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323125.2">
        <property type="nucleotide sequence ID" value="XM_003323077.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3Q4"/>
    <dbReference type="GeneID" id="10536643"/>
    <dbReference type="KEGG" id="pgr:PGTG_04662"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04662"/>
    <dbReference type="eggNOG" id="ENOG502QV5C">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033734_2_2_1"/>
    <dbReference type="InParanoid" id="E3K3Q4"/>
    <dbReference type="OrthoDB" id="19344at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051415">
        <property type="entry name" value="LAAT-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201:SF11">
        <property type="entry name" value="PQ-LOOP REPEAT-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16201">
        <property type="entry name" value="SEVEN TRANSMEMBRANE PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00679">
        <property type="entry name" value="CTNS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="249"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="304"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="334"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="367"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="174"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="400"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="195"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="5">
        <location>
            <begin position="416"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="443"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="467"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP78706.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="477" mass="52447" checksum="B64247A4DCEBD55D" modified="2012-04-18" version="2">MLLQLIKTFSTSRNLDQIPSRDNCHFEHDPTSMSLSIIIVLGILVSYLPQHYRIISSKSSHGISPWFLLLGSTSNTGSLVNAVTLQWGVVRCCQVLNPGECAESLLGIIQVFLQWACFNVILILSLIYFPRDLKYVRVVPVEIRERSDSPLMDRATNYSSQFLSRFFLKHNHRTTSSSSKQPRNPRESAPDQAVTSRSSHDSLASSQSTSSEHSTTFPSASSDSSAFDPSNVLPPSATRFTPLTLSKEYTISLVLAFVVFIHFLFSALVTLGLLLLLPKASLGEPVRTPPSHSAEHPTVRLLRIWASSSGILSLLLAACQYLPQIQETFQAKLVRALSIPMMLIQTPGSFLFVYSLAIRPGLNWTTWIVYLVTGILQGVLLILCVLWKIRQQANGVDDWGKPLARSTGSAKRTGDGLLGSEGYGEGQQEGPGRSERSPLLSALRPPSTFLSPSSSSTHRPASGKQRQIKDDSLDSSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3S8</accession>
    <name>E3K3S8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PFU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_04686</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78730.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323149.2">
        <property type="nucleotide sequence ID" value="XM_003323101.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3S8"/>
    <dbReference type="STRING" id="418459.E3K3S8"/>
    <dbReference type="GeneID" id="10536667"/>
    <dbReference type="KEGG" id="pgr:PGTG_04686"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04686"/>
    <dbReference type="HOGENOM" id="CLU_1210330_0_0_1"/>
    <dbReference type="InParanoid" id="E3K3S8"/>
    <dbReference type="OrthoDB" id="10265988at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.870">
        <property type="entry name" value="PFU (PLAA family ubiquitin binding), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015155">
        <property type="entry name" value="PFU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038122">
        <property type="entry name" value="PFU_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19849:SF0">
        <property type="entry name" value="PHOSPHOLIPASE A-2-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09070">
        <property type="entry name" value="PFU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51394">
        <property type="entry name" value="PFU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="PFU" evidence="5">
        <location>
            <begin position="75"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="174"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="175"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP78730.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="229" mass="24498" checksum="1F255735F093837F" modified="2012-04-18" version="2">MIRVFTRSEERLASSSELSEFEESVKTSSVPCATVGDVKKSNLPSVAVLLSCRGKKEGEVAMAKNESNGAVEAYQWDGMKGDWSMVGTVVNGIGSARKQLFEGKEYDYVFDFDIKDGEPPLKLPYNASDNPYTVAQKWLAKHELPSTYVNQVVAFINKNTSGVALGGPTAGSDPFTGSASYRPNPGQNQTQSNVGADPFTGAGSYRPNSGAPSVSKDIGLTIQLILQSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3Z2</accession>
    <name>E3K3Z2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78794.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323213.2">
        <property type="nucleotide sequence ID" value="XM_003323165.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3Z2"/>
    <dbReference type="GeneID" id="10536814"/>
    <dbReference type="KEGG" id="pgr:PGTG_04750"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04750"/>
    <dbReference type="HOGENOM" id="CLU_2134778_0_0_1"/>
    <dbReference type="InParanoid" id="E3K3Z2"/>
    <dbReference type="OrthoDB" id="10457931at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78794.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="113" mass="12671" checksum="FF9F58348F3C9BF7" modified="2012-04-18" version="2">MANTSYSNGRVPPLPFANSPEGLQESWLEPSLSADPNAEAFRLFHDNVCTESQFKNAYNQPTARSTGSESAPKPQLRAIYLDYHVYYQSVWNYPVGSDRVWKGKCAVNKAALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>E3K433</accession>
    <name>E3K433_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="9">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_04791</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. SKI2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="9"/>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78835.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323254.2">
        <property type="nucleotide sequence ID" value="XM_003323206.2"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K433"/>
    <dbReference type="GeneID" id="10536937"/>
    <dbReference type="KEGG" id="pgr:PGTG_04791"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04791"/>
    <dbReference type="eggNOG" id="KOG0952">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000335_0_4_1"/>
    <dbReference type="InParanoid" id="E3K433"/>
    <dbReference type="OrthoDB" id="5575at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000012">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052247">
        <property type="entry name" value="Meiotic_Crossover_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057842">
        <property type="entry name" value="WH_MER3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47835:SF3">
        <property type="entry name" value="HELICASE FOR MEIOSIS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47835">
        <property type="entry name" value="HFM1, ATP DEPENDENT DNA HELICASE HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23445">
        <property type="entry name" value="WHD_SNRNP200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-0469">Meiosis</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="162"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="382"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="59"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1001"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1087"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1171"/>
            <end position="1216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1241"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="59"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1188"/>
            <end position="1206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1269"/>
            <end position="1284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1295"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1319"/>
            <end position="1337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1379"/>
            <end position="1392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1400"/>
            <end position="1411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="1412"/>
            <end position="1424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EFP78835.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1444" mass="161896" checksum="5B7FDFB0048DA6EA" modified="2012-04-18" version="2">MDYSPARDGLQERYPLFKPPSPSQSESISDYFDDGLDEGSLNNLVDNIDRHAQYPSIQAQNLPFESNDQQSDRYYSPVPQMSRNSFNRNSSTEFPDQYESQSVYPDNPAVDSTYQDNHYQPVGPHRPRVSGPNLIPVTALPEPYTDFFSFSHFNSVQSECFPTVYGTNHNVLTNAPTGSGKTVIFELAILRMLEYNQVSKGVYMAPTKSLCAERFRDWSTRFGSLGVKCVELTGDSENAGLADAKLANLIITTPEKWDSMTRRWFEFSKLLNKIRLVCIDEVHMLNEERGSVLEVIVARMKTLGTNIRLIALSATVPNISDVAEWLGDGGVADQNEMALTGQAPAKTFIFGEEHRPVKLSKFVYGYTPCTNNSEMFNCLESRLMDHITTHSSGRPTLVFCGTRKSSLQAAETLSKSYQKMSSQGEKLPWEAPKSAGTFSDKKLTELGAQGIGVHHAGLDQSDRRQIESLFTLNKISVLCTTSTLSVGVNLPARCVIIRGTKTYRGGTSSRDGFEDYSELDLIQMMGRAGRPQFDDEGVAVVMTSQNDKMRIEKLVKSETMLESCLHLNLTEHINSEIYMGTIISRSSAIDWLENSFLSVRIKKNPKHYSISDDQVAPDQQLGKFAGAALDLLLEDGLIEEDEDQVIRPTELGEIMSKFCLRHKTFLGLARMKSSATMRNILEIVSGAEEYCTLRLRAGEGVAYKALNSHPEMKCPIPGKITQTWQKAVLGGIPLAEVKVENANPLMEVNIIWQHLPRICKCLVALSISRHDAAIKSCLEFLRSVTAKAWDDSPWVLRQLDSIGEKSVKRFVDAGIATIDKLQNTSNHRIEMILDRNPPFGTRIVRQARSMPKFFCTMETISETVVPEGVQVCVEITVGLSDTGETPVWKWKNYILMATVLVMTNDQEWIEFRTIQVKLLRETKQFNVECILVKPSQTIVTQVACTQLAGIGVSSRWKPRTSREHYPKPKTITDDQAASIEVLQGLNTKDLAASSTESECEFLEKPPKSMSKSSTQIARPVHKADKVCPKSQAPNGTAEPKRPVTEVRERLKNGRYKCAHRCKGACHHVCCRDGVSKPPKIREANKISLANSTDSQYSGSQKSLPLNPIRTTNITRTPEDWQKIRQAVPGSSFHANQPKLSGRIKPIKKIPEAIQVDRHDELPSLEELQEMSTIETEKTRPSKGIGGGNDRKAEVRSQHGNEKELDPVPKTAKRPKEVVGIEKFRNMKKIVEDFDERGIFHQQKEIPIPPPVNLPDVAVVEDTEPRDSKSLSLSPDISFPPCNNSHNDEERPQASEGEDETDELDEDWDIKIFESYLAPEESRKALEAKESAVTKRQIDQSYGNHSGPTPKKQKLTTTEYFKSLSPPDNEENWPQYPSRDNSKDPRENADDQSPRQPTFTREGEAQAESEKAPEDDEYDELDSDDSMTARMDEVLAWAESHVIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3K4T7</accession>
    <name>E3K4T7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_05573</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79252.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323671.2">
        <property type="nucleotide sequence ID" value="XM_003323623.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K4T7"/>
    <dbReference type="GeneID" id="10531076"/>
    <dbReference type="KEGG" id="pgr:PGTG_05573"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05573"/>
    <dbReference type="HOGENOM" id="CLU_043847_0_0_1"/>
    <dbReference type="InParanoid" id="E3K4T7"/>
    <dbReference type="OrthoDB" id="2507355at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP79252.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="395" mass="45921" checksum="C354A12930E9C571" modified="2012-04-18" version="2">MDEENTIDSVARMVSSFRSSNADGKTEEEYLEIIRNLIGHLHERVGLTKSLKIFDILPLESETLCRIEQIILPALKGKLRSLSLAFNSPNSQNDANSWYDVILNRLIEINNYLEQLETSMGPVRRDDVPKREIFPFRVIVISGQVFDLFSRGLCRLFAACQTFFDQLNFSNLSSSRINQGRDIARLTTVSVEKIDDLIPTLQKPLLRVAKEEWKAFVDGMNYQFKQPVQRPGLNVIDPDDWIWALGNEVRDKSFKAATPVSKLCRIFFNQLSRSTVNQPLIFDSPSMEMKEDQLNQFFHFSRKTDYTIHDFLEKSLSDPTDRQALQILTFRLEGRVVKLSQILERYWDGLWRKKDPQVDQDAIADAREWLECWSSLFFIATENFMQVTGCKELRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3K4Y7</accession>
    <name>E3K4Y7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05623</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79302.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323721.2">
        <property type="nucleotide sequence ID" value="XM_003323673.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K4Y7"/>
    <dbReference type="GeneID" id="10531126"/>
    <dbReference type="KEGG" id="pgr:PGTG_05623"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05623"/>
    <dbReference type="HOGENOM" id="CLU_011407_4_0_1"/>
    <dbReference type="InParanoid" id="E3K4Y7"/>
    <dbReference type="OrthoDB" id="2498701at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040521">
        <property type="entry name" value="KDZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18758">
        <property type="entry name" value="KDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79302.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="941" mass="108515" checksum="148C405819AA7BB1" modified="2012-04-18" version="2">MTLTVDQPPVSGTLAQRIRRRRNADRIAATRARFYGGQVANRNSVRRQQQHQEGVSNTIMNNDDHFNSTEDDTVDFTVHEDPTDDTDWITIPADDNDDFDRAAFADRERWRQEAKEVNWNTVLEPLHAYYMDLKARTKNWTDRANSYNDHTNCKCAPHLLTHRMVDLVDIHGAQPFTLALNEWLEPRSERLHARGKKHARELRKPFSATVDLYRTLENRTDELVFKALRLDPQEILASENCPACFGPRPPNAEDYPSQTRDKLIVCLDGNFQHRHHTKASRDYRPIETPRIFLPPSDFANMKTTIRDKEIELSPPARYSKMTQQADRCADSHKAADDKRNESTWKGCDDTGLIGCCCRHDSAIHMGNIYKSGEQRCLPIAIINKLLAECEGERQIGILYDIGCSLDKYMRARDLLGEHRSRLSFGTSVFHAYVHNWICQLDYHPRLNNGWGLSDGEGLERLWSYLSPLVSPLRYSTRNHRLASIAHRLKYHNWKGKRDLPAWLKRKFNAALKRRDEARLVLRGLQEIQNPYAASGVTYTKSFFEFQWQEQCAFQKQHTEVEQERQSKLVSLYKQEAVVELLRTRLRGPEIFLATEQQVLDLLASINEHSEQLRRQLDELTGEHGMQAVMNDEESKLLLLLWDAKADLFVQAVHIQAENRPITDSKTIGAHLGTKLKEKIFKASQARRPAVKKHIDHFNRCYLNYVTKFPDQTLSDAADYPLTYDNFVAFPLDHRFWNDGLYYHSKAPWAIDADVRAGITATLNLSRVQEEFQLLAQELCRAIGWGVSYYNRLTQTIDYLTGRVEAFNLDVEPRPDHFDSLSLYKFPRKHKYKVILRELRRRLASHGTLMDEWSEKVLWLWDRCQPAGNQGFILTWTDMMDKINRSNTINSDVLAGVDEEYEETVLEVDLDDGEDAEATLISSLGGDGASAGVNAGHPVDLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K5H3</accession>
    <name>E3K5H3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_06070</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324168.1">
        <property type="nucleotide sequence ID" value="XM_003324120.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K5H3"/>
    <dbReference type="GeneID" id="10533111"/>
    <dbReference type="KEGG" id="pgr:PGTG_06070"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06070"/>
    <dbReference type="HOGENOM" id="CLU_020173_0_0_1"/>
    <dbReference type="InParanoid" id="E3K5H3"/>
    <dbReference type="OMA" id="FAMSECA"/>
    <dbReference type="OrthoDB" id="2506864at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="77"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="508"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="15"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="77"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="122"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="517"/>
            <end position="583"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP79749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="583" mass="65365" checksum="C64A17B86294785C" modified="2011-01-11" version="1">MSMNTHPIDFEAVVSAALAKQARDLENDRNLALQQQAEQFKHSLAEIQNQMRSLSAAANLPSGGSLATISDSATVSATQTSNNTMPITPTQSSASKGSKKPKGKSKEATPLRGVRATSEPVATVKKTPTSASKTPKSGRVRQVTPSPLNRKRHPAQLVTSDIPKQYKTTKNSVPAKVDPHLSREFHQRFTSAEQVENAITHADSPQIISQEDILSLRQGRSGRFKLGRGMANIDEMQILYVHVVFSKVGIRVWGPNLEESHDSLFNSACRITALNTFRQLASSGAYHYMNINTSCLNELSFLVNAYNHYVHYVMESRFKKEMKEIGKHGKDEKKKSIQKNRERLCKSRHAYAVANKFPERYIRILNNTLAHSDDEFSPEHNIYIIKTLRYHSNNANIFFRRLDVEMLKADQLSGKQGRKRVRKLPKAPMASTFTKPPIGLPLDFYHRGWIKSLKETDQRLIPDSESVAFLPDAKLSLLPTRVPDEKLGDQVFCAKFRDLLVEPYGLNFLNGNKSNSSDDDSADGDNDDIEVDEDDTDQNEEEVEAEESLFLDEGDYGELYGADESDVENGVDEEGDQEDMDDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3K5R0</accession>
    <name>E3K5R0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05802</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79481.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323900.2">
        <property type="nucleotide sequence ID" value="XM_003323852.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K5R0"/>
    <dbReference type="GeneID" id="10531309"/>
    <dbReference type="KEGG" id="pgr:PGTG_05802"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05802"/>
    <dbReference type="HOGENOM" id="CLU_1886739_0_0_1"/>
    <dbReference type="InParanoid" id="E3K5R0"/>
    <dbReference type="OrthoDB" id="10268951at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172401" description="Secreted protein" evidence="1">
        <location>
            <begin position="29"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79481.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="154" mass="17923" checksum="96E0C2880B94168D" modified="2012-04-18" version="2">MLLTGFLQRSFIVLTVLTLITLPSGLFASSPQHCGLYFNGFSPVKDKYAVCENYSGVEYQCPNQNCNLRHVNWPDFKFLRCSEYSNGPDSPLTGYVIPWVFVSIMRAYKDQEYVDVKAYSHDTWYKCTWYHTDNYNKWKPFCTGCEPTNSIHRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3K5V1</accession>
    <name>E3K5V1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_05843</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        </source>
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    <dbReference type="OrthoDB" id="96345at2759"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
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    </evidence>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
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                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </reference>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="KEGG" id="pgr:PGTG_05979"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05979"/>
    <dbReference type="HOGENOM" id="CLU_1019898_0_0_1"/>
    <dbReference type="InParanoid" id="E3K687"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <source>
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    </evidence>
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            <dbReference type="EMBL" id="EFP79658.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
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                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
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                <person name="Brockman W."/>
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                <person name="DeCaprio D."/>
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                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
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                <person name="Yandava C."/>
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                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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            <dbReference type="PubMed" id="21536894"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP79935.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3K6W1</accession>
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            <fullName evidence="4">C2H2-type domain-containing protein</fullName>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
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                <person name="DeCaprio D."/>
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                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
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                <person name="White J."/>
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                <person name="Yandava C."/>
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                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EFP80060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324479.1">
        <property type="nucleotide sequence ID" value="XM_003324431.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K6W1"/>
    <dbReference type="GeneID" id="10534077"/>
    <dbReference type="KEGG" id="pgr:PGTG_05285"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05285"/>
    <dbReference type="HOGENOM" id="CLU_2147083_0_0_1"/>
    <dbReference type="InParanoid" id="E3K6W1"/>
    <dbReference type="OMA" id="PICCLEL"/>
    <dbReference type="OrthoDB" id="2505900at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP80060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="112" mass="12506" checksum="974E9B045ADEB6CA" modified="2011-01-11" version="1">MTKPYEEQREQKSRQGSDLTIYATPSLKRKPLEMARTEPSKEKKRAPAKLDLSSASGPVVEPPSPPPKPVTCPICTLQLPLLEAIKTHIREDHPQLYDPTLDQQQPAPQAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3K6Y2</accession>
    <name>E3K6Y2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PCI domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_05296</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit p55 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EFP80071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324490.1">
        <property type="nucleotide sequence ID" value="XM_003324442.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K6Y2"/>
    <dbReference type="FunCoup" id="E3K6Y2">
        <property type="interactions" value="709"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K6Y2"/>
    <dbReference type="GeneID" id="10534088"/>
    <dbReference type="KEGG" id="pgr:PGTG_05296"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05296"/>
    <dbReference type="eggNOG" id="KOG1498">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033860_2_0_1"/>
    <dbReference type="InParanoid" id="E3K6Y2"/>
    <dbReference type="OMA" id="AENEMFK"/>
    <dbReference type="OrthoDB" id="268763at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000070">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054559">
        <property type="entry name" value="PSMD12-CSN4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040134">
        <property type="entry name" value="PSMD12/CSN4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040896">
        <property type="entry name" value="RPN5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855:SF1">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10855">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 12/COP9 SIGNALOSOME COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01399">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22241">
        <property type="entry name" value="PSMD12-CSN4_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18098">
        <property type="entry name" value="RPN5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00088">
        <property type="entry name" value="PINT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="271"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP80071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="471" mass="53745" checksum="84A401AE4FF98995" modified="2011-01-11" version="1">MSPNSPKFGMLLGTGQVSCSVPRHTYSFGVIPSSPPISERGALRHRTRTRRSTTNDRHHHHYFDFNFKTSLTCKKNTVSTKMTETAPARKQEQDFTKEVDELIPEVDRLIKNGNLQQGLDKLLVLEKQTRNASDLSSTTRLLLEIVTRVFESKDIEGLCLQVHQLARKHGQLRQATTTMVEKVMSFIEKVDDHQQDKIKMIESLREVTEGKIYLEVQRARLTKQLAQIRESEGATKVANELMQELQVETFGSMERREKIDFILEQMRLLRIQQDWEKLAIVSKKINNKWLTEVENEDLNDLVKCFTTPELMRWPGIQELYGPILRKSKVFGPAGIAGLEGDLEDEEVDPTSNSGETRWQELHKRVVEHNIRAVSKYYTRLTIQRLSELLDLTIPESEATLAKLVSLKTVFAKIDRPSGIVRFSSGSSTPSHTTTSGESVLNQWNDDVGKLLGLVEKTVHLIQKEFALQGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3K8M1</accession>
    <name>E3K8M1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06728</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80772.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325191.2">
        <property type="nucleotide sequence ID" value="XM_003325143.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K8M1"/>
    <dbReference type="GeneID" id="10539792"/>
    <dbReference type="KEGG" id="pgr:PGTG_06728"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06728"/>
    <dbReference type="HOGENOM" id="CLU_045287_0_0_1"/>
    <dbReference type="InParanoid" id="E3K8M1"/>
    <dbReference type="OrthoDB" id="2498281at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="352"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP80772.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="543" mass="61241" checksum="6041A02033C4FB98" modified="2012-04-18" version="2">MDLPDFSDQLEKSETSEQFVDWLNEAFGFEPKTTDNPPSANPSTPTKGLAVTTVSPPSNQVNHQSRRLEESNSARRKDHVGSEQIKVSIEYRFYVLDTRPKPTPNTRVSNKRKAPPIEPEDKYIKITSDLGKVHIYWESSNTCLSDFKLAVIDTIAEKEGKRLGGHVRSQEEEGDILWYAIIPHGHMFVEKNKTTLEDDDIFEEFLAQAKGTRDGRKNTVNLLQRDPKVVAQVSTLTFLALETGPLALETDPDKKQALENLEKQDGGNEERTELNDHEPTAGASNSREIMNNIWMLRATHMPCERLTGSSELPVMICPSDSNRFIALTTDRLGLWARSMTKEEFLGISGRKREDAPDHLNHAVSDSAQPPGSRGTNQQFRSQSTEINPFPTTNHNHLATTTNFYPSQTSHFAMGPFFHPQMGWATQPWVWGPQQNIYQSHPVTPTAMAPNTLQTQTESVSSPACSEISVDINDYFHFCHVDPECEAVNKVLSEYGITHYSEFENFEAGELEALGVKKSNARSLVSRVQKFKQGLKKRHQQKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>E3KB08</accession>
    <name>E3KB08_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_07800</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81551.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325970.2">
        <property type="nucleotide sequence ID" value="XM_003325922.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KB08"/>
    <dbReference type="GeneID" id="10532932"/>
    <dbReference type="KEGG" id="pgr:PGTG_07800"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07800"/>
    <dbReference type="HOGENOM" id="CLU_054434_0_1_1"/>
    <dbReference type="InParanoid" id="E3KB08"/>
    <dbReference type="OrthoDB" id="2508351at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481:SF7">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="239"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="247"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="247"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="263"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP81551.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="303" mass="33240" checksum="2B020452DEB1135F" modified="2012-04-18" version="2">MPARTVQNPSALIGNVPVLDGNSVTFPAWRTRLEDLLAIQGVHDIVTGKLSRPETDYKDAKPVAQGSQRVYFAEESGTDWDSLSEVARATMKMTLSVDLAIRYKDLKPASLLFKTICDAYEKNTRARRMMLEDSFWCARHDPNQPIAKWIAKIRNAASDLKSVKLTPADQQICDRLLRGLDDTWKPIRDHLVYSPNEISLDDAIGALEAHEVSMQVTFDHSGENYAAAAVTKPKKRLGCWNCGQTGHHSSACPNPSLKNKSNATAKSAVARAGATSAAPLGNCGPSDDEDDDSFDKEIDVVWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3KB17</accession>
    <name>E3KB17_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Putative nuclease HARBI1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Harbinger transposase-derived nuclease</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGTG_07992</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Transposase-derived protein that may have nuclease activity. Does not have transposase activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HARBI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326162.1">
        <property type="nucleotide sequence ID" value="XM_003326114.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KB17"/>
    <dbReference type="GeneID" id="10533451"/>
    <dbReference type="KEGG" id="pgr:PGTG_07992"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07992"/>
    <dbReference type="eggNOG" id="KOG4585">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018552_2_0_1"/>
    <dbReference type="InParanoid" id="E3KB17"/>
    <dbReference type="OMA" id="RICAKWI"/>
    <dbReference type="OrthoDB" id="2687688at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026103">
        <property type="entry name" value="HARBI1_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF85">
        <property type="entry name" value="GH03217P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02086">
        <property type="entry name" value="PUTNUCHARBI1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="14">
        <location>
            <begin position="220"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="389"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="402"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EFP81743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="419" mass="47892" checksum="730CA1691E0A33FB" modified="2011-01-11" version="1">MRGLSERQQLIREVFLIMAYHHNEATEAILDGVMKLPSIPSFGAILFPGKPSLQFIWEMFFDDKAEVAEILEELLSHRFSSLRTTPTTHNDYDLAKLFDMRDNDFKQAVRTTKDGFLWLLDQIRHHPIFYSNSPRPQLPIPHQLALTLERLGSNGNGASVGRFSRNLSVGRGTVIKVSRRVIQAINEVSTNYIVWPNKERRAEISEVMTEEGFKGCIGFVDGTTIPLHQQPGLDGEVYWDRKKQYSINCQVICDCDRFITLFMTGWPGTCGDSLVFKNMKVHLEPDRFFDPGQYLIADSAYALSMTTIPAYKSPLSKINRNTEFNYCLAKSRVRNEHTIGILKGRWASLQQLRLSLYTPQHMVEIICWINCCVALHNMLAHLGDSWDELATPNDNDEPGDSEGNHEDNTEGHREEIQEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>E3KB30</accession>
    <name>E3KB30_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">VHS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_07811</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325981.1">
        <property type="nucleotide sequence ID" value="XM_003325933.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KB30"/>
    <dbReference type="FunCoup" id="E3KB30">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KB30"/>
    <dbReference type="GeneID" id="10532943"/>
    <dbReference type="KEGG" id="pgr:PGTG_07811"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07811"/>
    <dbReference type="eggNOG" id="KOG1087">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036827_3_1_1"/>
    <dbReference type="InParanoid" id="E3KB30"/>
    <dbReference type="OMA" id="YGSVHRQ"/>
    <dbReference type="OrthoDB" id="10068368at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007015">
        <property type="term" value="P:actin filament organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd14232">
        <property type="entry name" value="GAT_LSB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16980">
        <property type="entry name" value="VHS_Lsb5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008942">
        <property type="entry name" value="ENTH_VHS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044103">
        <property type="entry name" value="GAT_LSB5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045007">
        <property type="entry name" value="LSB5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002014">
        <property type="entry name" value="VHS_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47789">
        <property type="entry name" value="LAS SEVENTEEN-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47789:SF1">
        <property type="entry name" value="LAS SEVENTEEN-BINDING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00790">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00288">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48464">
        <property type="entry name" value="ENTH/VHS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89009">
        <property type="entry name" value="GAT-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50179">
        <property type="entry name" value="VHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VHS" evidence="2">
        <location>
            <begin position="45"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="405"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="163"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="441"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="457"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="475"/>
            <end position="485"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP81562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="519" mass="56643" checksum="139A432CD3D98A3A" modified="2011-01-11" version="1">MPSLFSDPTKAKELFTKPQTAVTDWIERICDPNRDSAQNDLNGFIIELVESINLQPTGPEEASRAIRKQLKHGRPTQQAKALTILEALVLNGGSRFKSTFADARLVECFKGLVSDPRTDSKVRRRLLIMMEDWEKAYGSDANMIVAASLFKTCGGRAKLEKLSPSSSSTQRKTSTQRSMITGIDKPPSTGLEPTPSISKAQLKMQKKQDKKRKEKLKSSPIDQRYRTELVLPASDSSSLLAFDLIKEKPRILQAVASASQSANNLVNRLQLIGPDQVARDLTVGGLVEKAKASQKALIRYIQIVSEHDSEGEYLGTLLNTNAQVVTALELYEDLSQSKPENSQVDRLADRLTKAKVTTPQPTSQSEPNLFDDQNYAVDTPAEHPASPAADLLGLDFNQTALMDGRDNLPAPLVPNSVGGTREAQPNYEPGTLSDYSDFDDSASSSDDTTEETTGSTKVSPPTTNNPFHQYLAPTSAETHPSLSSDLDQKEPLDPFADPFADTSASVATTHNSLITSRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>E3KB69</accession>
    <name>E3KB69_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Copper/zinc superoxide dismutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_07831</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326001.1">
        <property type="nucleotide sequence ID" value="XM_003325953.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KB69"/>
    <dbReference type="GeneID" id="10532963"/>
    <dbReference type="KEGG" id="pgr:PGTG_07831"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07831"/>
    <dbReference type="HOGENOM" id="CLU_116404_0_0_1"/>
    <dbReference type="InParanoid" id="E3KB69"/>
    <dbReference type="OMA" id="IFACVPA"/>
    <dbReference type="OrthoDB" id="159229at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024134">
        <property type="entry name" value="SOD_Cu/Zn_/chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001424">
        <property type="entry name" value="SOD_Cu_Zn_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10003">
        <property type="entry name" value="SUPEROXIDE DISMUTASE CU-ZN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00080">
        <property type="entry name" value="Sod_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173398" evidence="1">
        <location>
            <begin position="29"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="Superoxide dismutase copper/zinc binding" evidence="2">
        <location>
            <begin position="51"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP81582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="177" mass="19152" checksum="0290701F2C271069" modified="2011-01-11" version="1">MNPPTIKLVHFLGLLLFIFAYVPAIVRAAGRAQPTCPKGYSAQVIGIQGITGKVTFKPTTAGIEVTVVANGFERMEKHKCHIHVNRIQGGNCYTAGANYDLNSKQPNCPLRTPQDQCQTGDLTGKGGDLEPKKPGVEVSMRYTDKILKVQDLVGHSVVFHDQGGDFIGCGNIECVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBU2</accession>
    <name>E3KBU2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Uracil-DNA glycosylase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_07986</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81737.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326156.2">
        <property type="nucleotide sequence ID" value="XM_003326108.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KBU2"/>
    <dbReference type="STRING" id="418459.E3KBU2"/>
    <dbReference type="GeneID" id="10533445"/>
    <dbReference type="KEGG" id="pgr:PGTG_07986"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07986"/>
    <dbReference type="HOGENOM" id="CLU_608500_0_0_1"/>
    <dbReference type="InParanoid" id="E3KBU2"/>
    <dbReference type="OrthoDB" id="565731at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008263">
        <property type="term" value="F:pyrimidine-specific mismatch base pair DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004844">
        <property type="term" value="F:uracil DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd10028">
        <property type="entry name" value="UDG-F2_TDG_MUG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.470.10">
        <property type="entry name" value="Uracil-DNA glycosylase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015637">
        <property type="entry name" value="MUG/TDG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12159">
        <property type="entry name" value="G/T AND G/U MISMATCH-SPECIFIC DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12159:SF9">
        <property type="entry name" value="G_T MISMATCH-SPECIFIC THYMINE DNA GLYCOSYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP81737.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="450" mass="50233" checksum="86801FA12F2B0EE5" modified="2012-04-18" version="2">MKGQNPEPRGLYKPLAYPTASYSSCLHWKISIVRPTKSEYTYDLRDRTSEALAGPVDMSPGTLQSKYFASKASAKSKDDGSSGCHFSGNRTERVVTRALAKVGIPFKRNRSLSASSFSASIACEKDTCDDHEKVMISKHFKPGLPAHTPRKQKKARQYAAPAVYQHLNHLPDLIAPSLDVSVSLLLGNYRKPPSPRKTRNVQTNESLTHSDQLNKSWMSIGQNFALLCTSIQPFLEIFISECMSLLSKTHRFTPHRLSPHDDHLLPSEFGLGLTDLVCRPTAEANELSGSERLQGVPVLLGKIRRFQPRFLCFVGIKQYHDLCSYLKKKLRKSRDLLPSIWQSPSQKTVVGLQNVVFRSQKTSRINKGQSKSTAADADQYSHTFVFVCPSTSALVRNYPEKDRVACFRKLLELKNRASLNPDSSATPIASGSSRTKLCLEYIEIDDALLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCE0</accession>
    <name>E3KCE0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_08137</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EFP81888.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326307.2">
        <property type="nucleotide sequence ID" value="XM_003326259.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCE0"/>
    <dbReference type="STRING" id="418459.E3KCE0"/>
    <dbReference type="GeneID" id="10533925"/>
    <dbReference type="KEGG" id="pgr:PGTG_08137"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08137"/>
    <dbReference type="HOGENOM" id="CLU_1138480_0_0_1"/>
    <dbReference type="InParanoid" id="E3KCE0"/>
    <dbReference type="OrthoDB" id="2509423at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13948:SF3">
        <property type="entry name" value="FI21118P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13948">
        <property type="entry name" value="RNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP81888.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="244" mass="27777" checksum="EAE2E82EE0602BCA" modified="2012-04-18" version="2">MNRPEKASKPLPYCHHHPYHSSHSTRTPKEVVRRPSNPPSHENNHLTKPSNHPEQFAFNINQPKSKVSQSIILKGIPGLADNSSVFKFLESAGAAVCLVNVIYNPKTSRSRRYAYAHFFGLDRAKEFVKPRFPLMSWKEPDNSSHGDYYRTLKIQIDYSTEPPEVKQNRRNDGDDDVGQMPISILLLRGLNKKTTLQDIHTSLQMICDPPESIPIQNIMLVMDQATCTSCVYAFVHFPTVKKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCQ4</accession>
    <name>E3KCQ4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_07603</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82206.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326625.2">
        <property type="nucleotide sequence ID" value="XM_003326577.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCQ4"/>
    <dbReference type="GeneID" id="10529578"/>
    <dbReference type="KEGG" id="pgr:PGTG_07603"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07603"/>
    <dbReference type="HOGENOM" id="CLU_2428105_0_0_1"/>
    <dbReference type="InParanoid" id="E3KCQ4"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP82206.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="91" mass="11021" checksum="3E96F3DA027701DA" modified="2012-04-18" version="2">MIIRFYIKAVIRKSVHRTQQHWIHKLSLDPPSVSWCNQPYLFYHYPPPLKSKLLTHRLHNSNSNFGNRISKNETYIYWSRFDRFCFSFGGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDA7</accession>
    <name>E3KDA7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">tRNA ligase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_07519</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82122.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326541.2">
        <property type="nucleotide sequence ID" value="XM_003326493.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDA7"/>
    <dbReference type="FunCoup" id="E3KDA7">
        <property type="interactions" value="213"/>
    </dbReference>
    <dbReference type="GeneID" id="10534653"/>
    <dbReference type="KEGG" id="pgr:PGTG_07519"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07519"/>
    <dbReference type="HOGENOM" id="CLU_010316_0_0_1"/>
    <dbReference type="InParanoid" id="E3KDA7"/>
    <dbReference type="OrthoDB" id="276239at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003972">
        <property type="term" value="F:RNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019039">
        <property type="entry name" value="T4-Rnl1-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015966">
        <property type="entry name" value="tRNA_lig_kin_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015965">
        <property type="entry name" value="tRNA_lig_PDEase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32004">
        <property type="entry name" value="TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32004:SF1">
        <property type="entry name" value="TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09511">
        <property type="entry name" value="RNA_lig_T4_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08302">
        <property type="entry name" value="tRNA_lig_CPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08303">
        <property type="entry name" value="tRNA_lig_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="T4 RNA ligase 1-like N-terminal" evidence="4">
        <location>
            <begin position="122"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA ligase kinase" evidence="3">
        <location>
            <begin position="485"/>
            <end position="669"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA ligase phosphodiesterase" evidence="2">
        <location>
            <begin position="675"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="883"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="886"/>
            <end position="900"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF08303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP82122.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="933" mass="104429" checksum="D3262927AFEE0F2D" modified="2012-04-18" version="2">MATGASPLRKFQSQPTISQQTDLLIQTLLHFGLSNQNNNSNNRDSRTQIIELSVDELAVALEKLSAGQGDSNQPDDPKLKKLVRSRVYDIHSSHLDALVSVRSWTMTEHVYRRVPCPFPTMARGLFTRENLHPDRALGQGRHAIVARGYDKFFNIDELPSTKWEALASRSQAPYHLTLKTNGCIIFLAALTPSDLLVTSKHATGGSKEEDEDEPMTHSAVGERWVERHLAQVGLSRAELARELWEANATAVAELTDDDFEEHVIATPADQVGLNLHGVNLNTPELITYPPEIVAQCAKRWGMHPTPFTTVNDIEAVKEHCRMIQTDGWAGRQGHIEGVVVRGTPRDQQPSPGPNPAATSIFWKVKFEEPYLTYREWRELTRKMLHDLSDSEELAKVLKVNVKKIHKPETRLYVHWVLQELLNRPQTFDLWQKNRGIVATREAFFEWRNSQDALKIIDQLGSSASQKPSTGAGTEVEEDQHFDKTLLVPIGIPGCGKTTLAIALKHLTKCSHTQSDDITTKKTGPAFIENVKSLLLDKSGPSLVIADKNNHLKTHRTALVELIAKLKEGIQVKKGKGKGSRETELVRMKVRMVAVTWDLEAHSSGELHQIAAQRIVQRGENHQSLRPELTSGGLEAHDAILWRFLKTLEDFDPDINPEDQPFRHIIQLKFADSIENNLEDLCAQLSKICPGLLAPEDRIDSQKMKNALETARAYKPSIKKEMKVEAALQSPRYFGVKLDVDLKKLVGELLEAFDQTSQEPTQKAGHKMFTELVKNRRITATPHLTLVHQNQTEESEEMKQLWDEYQGRVLARKVAFGLLLGPKLIWNNRVMILEARVRTQSPGDSATDPAPTSIRYHVTIGTADESIRPVEGFDLLHHFFRSVPHASHSSTQPAPSPQTHATLDAGKQDADRAGEVLFLDVKTVSVGGTLKGLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDL6</accession>
    <name>E3KDL6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08408</name>
        <name evidence="3" type="ORF">PGTG_13733</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="protein sequence ID" value="EFP87929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003326823.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332348.1">
        <property type="nucleotide sequence ID" value="XM_003332300.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDL6"/>
    <dbReference type="GeneID" id="10529831"/>
    <dbReference type="GeneID" id="10534908"/>
    <dbReference type="KEGG" id="pgr:PGTG_08408"/>
    <dbReference type="KEGG" id="pgr:PGTG_13733"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08408"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13733"/>
    <dbReference type="HOGENOM" id="CLU_032241_1_0_1"/>
    <dbReference type="InParanoid" id="E3KDL6"/>
    <dbReference type="OrthoDB" id="2507728at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="250"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="269"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP87929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="450" mass="49805" checksum="F5AAB8E6D14FF2E4" modified="2011-01-11" version="1">MDIPLASAQDESTEPKRRRTKPEMIIHRAELAKKKEALEAAKLLKKKPHPKAVKTAAGTSGSKSTKPTGAADNNPQFIASDFELICTYLEDPHHYTEIYGNGPRTGVGQQVMTKSAAYDRFAIFMNDKTHKRLHLDGKLLRQRIEAYKKRFVAAKRWADNTGAGIEEGEDIVSIDVLLEGKCPCYERMSALFGEKPNVTPAAQYESQQGMSLYNRPVDNDNPDNPSNPEMFYPGWEPTQEVLDQTTHVNESPDLATSGQANQTSGVDQSNDNPDLPDSLLDAPPSHQPTPPLDSAPVSAMPSTSATPGHQLGSSQPRRTMASMLADLRANISREEEDEVDDAEPSGSAAPRGRFVNQVDRSIPAPRRETNNPKGKSTVAAAFGSSSDKKFAYLDKHMEMEKEKFEWEKSKYKSEQGDITTRENAKMRAAEKWISQGKSPSDIEILMRTVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDS4</accession>
    <name>E3KDS4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cell survival pathways protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_08466</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SVF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326929.1">
        <property type="nucleotide sequence ID" value="XM_003326881.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDS4"/>
    <dbReference type="FunCoup" id="E3KDS4">
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    </dbReference>
    <dbReference type="STRING" id="418459.E3KDS4"/>
    <dbReference type="GeneID" id="10529889"/>
    <dbReference type="KEGG" id="pgr:PGTG_08466"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08466"/>
    <dbReference type="eggNOG" id="ENOG502QQY3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030205_2_0_1"/>
    <dbReference type="InParanoid" id="E3KDS4"/>
    <dbReference type="OMA" id="AFWPRCV"/>
    <dbReference type="OrthoDB" id="2590239at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051385">
        <property type="entry name" value="Ceramide-binding_SVF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033394">
        <property type="entry name" value="Svf1-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013931">
        <property type="entry name" value="Svf1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47107:SF1">
        <property type="entry name" value="CERAMIDE-BINDING PROTEIN SVF1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47107">
        <property type="entry name" value="SVF1-LIKE PROTEIN YDR222W-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08622">
        <property type="entry name" value="Svf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17187">
        <property type="entry name" value="Svf1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159245">
        <property type="entry name" value="AttH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Svf1-like N-terminal" evidence="4">
        <location>
            <begin position="53"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Svf1-like C-terminal" evidence="5">
        <location>
            <begin position="242"/>
            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF17187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP82510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="475" mass="51835" checksum="B523E0ADE8F8387E" modified="2011-01-11" version="1">MSWFSVEPPQAQSPTQPAVLVHPTSSRYQPGNLLGELKDEDTSWLAQSSGFVTETQTFYITLPGNKLAMCQVIHSAIGLWYPQIQFTFRFVDQGSGAHHWKSTNVTNFKTPPASSGSSSVKYDRRSCKADQFSILLDPAQPDRYTITGKHDDSIHIQFSVTRMADVPGWKLGQDARGGLTYFGRWASKEPSKSGPDYGAGSDGYLVHRFWPRCSVSGRLLIDGKTVELDGSRAIFVHAIQGMRPNLIASRWNFCNFQSVRPLEPLSEGAEDTDPGVSLTLMEFTTVPGSYGPPQTITVGSIVVGDKLVGICCSPNPSELVDSKTTHQAPLLHDVDTGYQVPSSIKFEWMAPVLANALGSSAPSTDSSPLSATAHLVLDLEAKRISEKESYVTKGLIEKVDVMAQIPYLVKKVISAVAGARPYIYTWLNPVEAHITLPNSALPNGFNVKTEAVEGEASKFTIHGHLFNEATFISDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KE43</accession>
    <name>E3KE43_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_08989</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327212.1">
        <property type="nucleotide sequence ID" value="XM_003327164.2"/>
    </dbReference>
    <dbReference type="GeneID" id="10544500"/>
    <dbReference type="KEGG" id="pgr:PGTG_08989"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08989"/>
    <dbReference type="HOGENOM" id="CLU_256138_0_0_1"/>
    <dbReference type="InParanoid" id="E3KE43"/>
    <dbReference type="OrthoDB" id="2498893at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="993"/>
            <end position="1042"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="442"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="565"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="659"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="716"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="765"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="904"/>
            <end position="948"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="450"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="467"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="497"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="570"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="665"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="769"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="921"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="938"/>
            <end position="948"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP82793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1469" mass="164035" checksum="C22BA754BA4442D6" modified="2011-01-11" version="1">MGTLQAQAASLDSDPTSRLKRQLSECSKVRRPLDATPLGLTETYEEAKLNLQSLASGHSSNNFHPRAFTSKRATASLPYVTHLVPRSILTESLPQKPAENYLFKALPRPPTEGQLAQSEWTTDLGRSSSAPTRVRFADPMVQYNPTPAARESCEFSQFELSKNPHTGRKTDLMAFPKSCIKSTGQYHQVQGKHSLCAKRTDSSPPARLCDSPLEEEFRASISFMSELIFPEDPDQGAGSEPSVAAGAGGAKGLSSKAIPLSISSNVEKDVRSRSTDHELELRKLKQFKESIRNMPSGPARSALPIPAGVFQSSSAPSTPSNQGSYQRKQDKPLHVPRQLEKESSQYANDYRDKNTPKFSMSPKIKRIEVSNLQGTATQLQQGRKILPTSGKVTITGRSCPLEGSKGFAPTYPSQPPGHRKLDAIPDLPSPIVDDLTQCRRTIVTPQVQTEETRNLGKRSFRERTDRSPTVSPQQVSPLTLPPRGSRKQSTICPDSPSWKESRSPCKRLKDLQSVGRTGPRDINTIVTGDSEGPRSDLEPGEISPRLDSEYMLNTEMSLEDINADARFSNPPSVSSEAPSTSSRTDFSPVPSPPAPITTTLESWRHDLTPANLAEACHQYGQSETTKPCIIDAQDGTSQCQTPFQCVVVSETEELIPRALEPSIYDEAKSPRDLESKESFDRPSCRKEPEPVPRELLGMFGEDMIEDQIVEKAQETTMKQTEDTIETGSDRSEAAVTTSTRPQCFCTCSKRWENWNLYLRDKEDEPMKCSSQSSSAGRSSISSHTDDSFLWHSAPQIAIIKPEECLFSELKSPPIYLEGLRFSTSRTSVDCEPGADNQFIVETTNVENEEIIRLDADGLPEPIQTTRCVNSPPEDRKQMSHPSIQALQNDRLQNQTTDSNFTAIIMKPPPMKKRSLSCKGSETNDRPETPRRYRRRRDHCTSKTNGKECEDRAPVSTLEVSSSSTLKESINNSTCQSIPFDLGSGKSTIITKFEFSILSFPQTILHRILVELDYLDFFRLSQVSHVLRTGFSVGEVQEVILKVFLGDLEYGDSLSSSSRSRTITHRFLSPNPTVELDGGDVVLENKTTAVLLGDSRFNSAGGAIPDPFPSAMTLGKRNMHTSSPLEINLQELHAFHYFRQAGEKSFLELANRPTSHRPSALSAIQAYCRLHNKLVIRARLRFEDPSNPIHLQSHQFRSRKPHPEIFSPGKVVVFRLWIPSEKKPTSKAELLKCEMEMLRSGIQAFARRGDIFWNAALGSSDNTGRFIYDGQDLQELQVLWDSVGHLPDWLNMFLFPPSFYHHTIKSSNLNPIFYLDLTDFKEQLINSLSLVDHHQPHETAEDQSQQGMLNYLGLVEIRAGTKTGVLEGIECFSDTHHEYILPECIHPDWVGKLMVEIQVSSLEKLDQLLSKFNKAKPEDFFSLGFDLASSPEETRHRTVSRLKSAQLSPWRIIRQKSSPGMVWIRLPIRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3KES0</accession>
    <name>E3KES0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">glutathione-specific gamma-glutamylcyclotransferase</fullName>
            <ecNumber evidence="1">4.3.2.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_08970</name>
        <name evidence="4" type="ORF">PGTG_13866</name>
    </gene>
    <organism evidence="3 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <reference evidence="3" key="3">
        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <dbReference type="EC" id="4.3.2.7" evidence="1"/>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="DS178307">
        <property type="protein sequence ID" value="EFP87495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327193.1">
        <property type="nucleotide sequence ID" value="XM_003327145.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331914.1">
        <property type="nucleotide sequence ID" value="XM_003331866.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KES0"/>
    <dbReference type="FunCoup" id="E3KES0">
        <property type="interactions" value="329"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KES0"/>
    <dbReference type="GeneID" id="10530157"/>
    <dbReference type="GeneID" id="10538398"/>
    <dbReference type="KEGG" id="pgr:PGTG_08970"/>
    <dbReference type="KEGG" id="pgr:PGTG_13866"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08970"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13866"/>
    <dbReference type="HOGENOM" id="CLU_070703_0_1_1"/>
    <dbReference type="InParanoid" id="E3KES0"/>
    <dbReference type="OMA" id="DHREKDG"/>
    <dbReference type="OrthoDB" id="5894at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061928">
        <property type="term" value="F:glutathione specific gamma-glutamylcyclotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.490.10:FF:000032">
        <property type="entry name" value="Gamma-glutamylcyclotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.10">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006840">
        <property type="entry name" value="ChaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036568">
        <property type="entry name" value="GGCT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12192">
        <property type="entry name" value="CATION TRANSPORT PROTEIN CHAC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12192:SF2">
        <property type="entry name" value="GLUTATHIONE-SPECIFIC GAMMA-GLUTAMYLCYCLOTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04752">
        <property type="entry name" value="ChaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110857">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP82774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP87495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="202" mass="22899" checksum="46461443CF80EFE7" modified="2011-01-11" version="1">MAIDRIFGYGSLIWKPPPFVTGRTPCYVKGYTRRFAQFSHDHRGTPENPGVVVTLVPNSEWLNLPGADEYPSRLVWGVMYTIDPNHAEEVHAYLDEREKDGYELMEVDVFVIKDGQEECYGKSSIYVGHTTNPSFCRPTKLEELASRIHKSIGPSGPNKEYLYHLKREVEALCPEANDPYLSDLEGLVKQLDQTSCSSALRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KG53</accession>
    <name>E3KG53_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_09265</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EFP83312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327731.1">
        <property type="nucleotide sequence ID" value="XM_003327683.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KG53"/>
    <dbReference type="GeneID" id="10545027"/>
    <dbReference type="KEGG" id="pgr:PGTG_09265"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09265"/>
    <dbReference type="HOGENOM" id="CLU_625743_0_0_1"/>
    <dbReference type="InParanoid" id="E3KG53"/>
    <dbReference type="OrthoDB" id="2498256at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173854" evidence="2">
        <location>
            <begin position="22"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="301"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP83312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="364" mass="40438" checksum="E73E5D248FB8CBAE" modified="2011-01-11" version="1">MKIAIRHLLLVFLSLIASGASLDDLPRVTRDFLSRCTGTTGGGVTTPQDMDGIDLQAKSPTLQGTAKEIDFSSNLTSSPYGARQRGDLPSYSPPLPPAEYKEKDSFPGYLIGEVPRGSRLVPSKAHIRIAATRRKREDKCFVQSEIYPEAKKQLRTLDLLKGFGQYTPPDPSASLDEVSRQAGSFHRVAETDATQFQIDNEINVVPINPVRTNQELMKALENQIKIPAFKRTRKDKTSVRSSLCSTFNTYQDFDFPSRSVRGSTSEKLGLIPLMNGQEPASHQKDTLIRETDRNRQIHSPLESTGNLKASPTTTDELAPYQVEIGVETLKNPSKINALTSQAASTRDDRFLRLEFTLQVPYNEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>E3KH31</accession>
    <name>E3KH31_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">asparaginase</fullName>
            <ecNumber evidence="1">3.5.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_09319</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the asparaginase 1 family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.1" evidence="1"/>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83606.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328025.2">
        <property type="nucleotide sequence ID" value="XM_003327977.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KH31"/>
    <dbReference type="SMR" id="E3KH31"/>
    <dbReference type="STRING" id="418459.E3KH31"/>
    <dbReference type="GeneID" id="10532290"/>
    <dbReference type="KEGG" id="pgr:PGTG_09319"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09319"/>
    <dbReference type="eggNOG" id="KOG0503">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019134_3_0_1"/>
    <dbReference type="InParanoid" id="E3KH31"/>
    <dbReference type="OrthoDB" id="542841at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006528">
        <property type="term" value="P:asparagine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd08963">
        <property type="entry name" value="L-asparaginase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1170:FF:000003">
        <property type="entry name" value="60 kDa lysophospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:001156">
        <property type="entry name" value="L-asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.40:FF:000001">
        <property type="entry name" value="L-asparaginase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="entry name" value="L-asparaginase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020827">
        <property type="entry name" value="Asparaginase/glutaminase_AS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040919">
        <property type="entry name" value="Asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041725">
        <property type="entry name" value="L-asparaginase_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027474">
        <property type="entry name" value="L-asparaginase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707:SF28">
        <property type="entry name" value="60 KDA LYSOPHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00710">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17763">
        <property type="entry name" value="Asparaginase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500176">
        <property type="entry name" value="L_ASNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00139">
        <property type="entry name" value="ASNGLNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00057">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00144">
        <property type="entry name" value="ASN_GLN_ASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="L-asparaginase N-terminal" evidence="9">
        <location>
            <begin position="15"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Asparaginase/glutaminase C-terminal" evidence="10">
        <location>
            <begin position="309"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="580"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="6">
        <location>
            <begin position="613"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="81"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="89"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00061199"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP83606.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="652" mass="71503" checksum="CDB5A1EDE5DA8AAA" modified="2012-04-18" version="2">MMKHHSQIRDANESRVLVLYTGGTIGMLSGPKGYSPLPGYLSEHLRSQPRFHDPSSSSLCTNSQTVDAFRSWQSLVSLNSPSSSSTSPIYPPANNPPSPASAEAGPTTDETSRFNGNQLKVITENGSQWLDSLVMQESHGCPKIRYAILEYERLIDSSEIEISDYLRICNSIQSNYYLYDSFVILHGTDTMAYTASALSFLLENLGKNVIITGAQIPLSCPRNDAVENLLGALQISGTYLIPEVSLYFNHRLWRGNRSMKVSSVEFDAFKSFNLEPLVKLGCNVEVRWDLIYRPVERQAFRVHQAMCENVAVLRLFPSITPASVRAFLAPPIQGIVLETFGSGNAPRKPALLQAFKEAADRGVVIVNVTQCVVGTVSSDIYETGRALAAVGITGGRDMTTECALAKLAYLLSKPSLSPAKIRTLISQPLRGELTLPARVQFQPQPVEDRLNWLMKHITSLQLDNRDPPLSDGRKEEIGNLNIRESLPQIPATIISDDSAPLLQRSSTHLGRLAVYQDVGVELETSEMLQKEQDLTERSLLPIALTAASARDDSSLASLLAICSDQIQTQGLLNLRTATGLGQTALHLAAFYGRLKNVELLLDHGASVHLRDDTGHTALFYAINNQHKACVDLLVKAGAHLTNFEQDEDCSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHL4</accession>
    <name>E3KHL4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">acetyl-CoA C-acyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_09502</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>an acyl-CoA + acetyl-CoA = a 3-oxoacyl-CoA + CoA</text>
            <dbReference type="Rhea" id="RHEA:21564"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58342"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="2.3.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Lipid metabolism; fatty acid metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the thiolase-like superfamily. Thiolase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.16" evidence="5"/>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328208.1">
        <property type="nucleotide sequence ID" value="XM_003328160.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHL4"/>
    <dbReference type="STRING" id="418459.E3KHL4"/>
    <dbReference type="GeneID" id="10532476"/>
    <dbReference type="KEGG" id="pgr:PGTG_09502"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09502"/>
    <dbReference type="eggNOG" id="KOG1389">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031026_1_1_1"/>
    <dbReference type="InParanoid" id="E3KHL4"/>
    <dbReference type="OMA" id="DIIMGCV"/>
    <dbReference type="OrthoDB" id="5404651at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00751">
        <property type="entry name" value="thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050215">
        <property type="entry name" value="Thiolase-like_sf_Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020615">
        <property type="entry name" value="Thiolase_acyl_enz_int_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020610">
        <property type="entry name" value="Thiolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020617">
        <property type="entry name" value="Thiolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020613">
        <property type="entry name" value="Thiolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01930">
        <property type="entry name" value="AcCoA-C-Actrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43853">
        <property type="entry name" value="3-KETOACYL-COA THIOLASE, PEROXISOMAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43853:SF9">
        <property type="entry name" value="ACETYL-COA C-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02803">
        <property type="entry name" value="Thiolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00098">
        <property type="entry name" value="THIOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00737">
        <property type="entry name" value="THIOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00099">
        <property type="entry name" value="THIOLASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="9">
        <location>
            <begin position="18"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="10">
        <location>
            <begin position="285"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="7">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="364"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00024073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP83789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="408" mass="42907" checksum="9D8A49EE4DE3C5D9" modified="2011-01-11" version="1">MSLRHGLSAVTRKSPSDVVIITALRTAIARSGKGSFKDSYPEELLSHVLDASRQRLEGMSVRAGQVQDIAVGTVLMELGGAKSGRLAALHAGYPYQTTFRTVNRQCSSSLQALTDIAATIDSGAIDCGIAAGTESMTRNYGSRAIPTDLSPSLRESPVQDARDCLLPMGLTSEAVAEKYGIARTALDEFSLSSHLKASAAEQDGLFADEIVPIEVNYVTEDGSTVKKTVSKDEGIRHGLTLEKLNGLKPAFKPDGRSTAGNSSQISDGASAVTLMRRSMAEQLGLKPLAKFVGSNVVGVPPRIMGVGPAFSTPVLLKRFGLEVGDIDLFELNEAFASQALMTIGHLNLDITKVNPKGGAIAIGHPLGATGGRLVSSLIHELRRTEKKIGVAALCCGTGFGKSSLFVAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJE3</accession>
    <name>E3KJE3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">2-(3-amino-3-carboxypropyl)histidine synthase subunit 2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Diphthamide biosynthesis protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Diphtheria toxin resistance protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">S-adenosyl-L-methionine:L-histidine 3-amino-3-carboxypropyltransferase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_10138</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Required for the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2. DPH1 and DPH2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising DPH3 and a NADH-dependent reductase, predominantly CBR1. Facilitates the reduction of the catalytic iron-sulfur cluster found in the DPH1 subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the 2-(3-amino-3-carboxypropyl)histidine synthase complex composed of DPH1, DPH2, DPH3 and a NADH-dependent reductase, predominantly CBR1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the DPH1/DPH2 family. DPH2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178290">
        <property type="protein sequence ID" value="EFP84418.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328837.2">
        <property type="nucleotide sequence ID" value="XM_003328789.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJE3"/>
    <dbReference type="FunCoup" id="E3KJE3">
        <property type="interactions" value="734"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KJE3"/>
    <dbReference type="GeneID" id="10545846"/>
    <dbReference type="KEGG" id="pgr:PGTG_10138"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10138"/>
    <dbReference type="eggNOG" id="KOG2648">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015210_1_0_1"/>
    <dbReference type="InParanoid" id="E3KJE3"/>
    <dbReference type="OrthoDB" id="449241at2759"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090560">
        <property type="term" value="F:2-(3-amino-3-carboxypropyl)histidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11840:FF:000002">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11860:FF:000001">
        <property type="entry name" value="2-(3-amino-3-carboxypropyl)histidine synthase subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11840">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11860">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042263">
        <property type="entry name" value="DPH1/DPH2_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042265">
        <property type="entry name" value="DPH1/DPH2_3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00322">
        <property type="entry name" value="diphth2_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762:SF2">
        <property type="entry name" value="2-(3-AMINO-3-CARBOXYPROPYL)HISTIDINE SYNTHASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01866">
        <property type="entry name" value="Diphthamide_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01121">
        <property type="entry name" value="Diphthamide_biosynthesis"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00032">
        <property type="entry name" value="Radical_SAM_3-amino-3-carboxyp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="472"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="558"/>
            <end position="587"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00080784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EFP84418.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="587" mass="65721" checksum="FF6A8A447189AB0C" modified="2012-04-18" version="2">MATIDEGPSASKESQTDILSDYKILETVEWIARHSFTIVGLQFPDELLSISVPIFRSLRSRLDSDEIELYIMADTTYGSCCVDHVAAQHVHAQAVVHYGQACLSITPSLPVFYVLPKFPISDEMVERTAKRMVESLYSMDPTFDVSNDSNQILLMYDVGYHWKSESITNCLKTNFNLPIVRHELGLEETKNLLIPPHSSASVAPSINQCSEQTCCQSSCQDSKPCSNQPIACTTTTTTTTPTTNIPISQEAGSSVLPSNSRGNKTDFSSYQVVFYVGSESSRLTHFLVTHPHLPVIAVDPNQTEEDSNDHSRLQQNNRTKKLLMRRYATIQRARDADVFGILVGTLGVRHYLEMINRTKKLIEEVFKKKAYIISVGKLKPEKLMNFAEIEAWVLVDCPESSLLTLDGKTGERFDPKQFGAPIITPWELEIALNSCIEPSNDDQSENQKRVWDGKLVLDFEQILSVWQAEDSFDTSAPKNGPPSPHSGDSDQQEGPVFSMVTGGYKYRRNWNDNHESESQSKEVQKLEMKDKQELIESVKGAASQYALSHRTYFGLEPRFGLDPPSKVSAGRYGIPQAYQDDHPDSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3KKP2</accession>
    <name>E3KKP2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Catalase core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_10338</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the catalase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178292">
        <property type="protein sequence ID" value="EFP84867.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329286.2">
        <property type="nucleotide sequence ID" value="XM_003329238.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KKP2"/>
    <dbReference type="STRING" id="418459.E3KKP2"/>
    <dbReference type="GeneID" id="10526841"/>
    <dbReference type="KEGG" id="pgr:PGTG_10338"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10338"/>
    <dbReference type="eggNOG" id="KOG0047">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010645_2_0_1"/>
    <dbReference type="InParanoid" id="E3KKP2"/>
    <dbReference type="OrthoDB" id="6880011at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004096">
        <property type="term" value="F:catalase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042744">
        <property type="term" value="P:hydrogen peroxide catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.180.10:FF:000016">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.180.10">
        <property type="entry name" value="Catalase core domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018028">
        <property type="entry name" value="Catalase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024711">
        <property type="entry name" value="Catalase_clade1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011614">
        <property type="entry name" value="Catalase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010582">
        <property type="entry name" value="Catalase_immune_responsive"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020835">
        <property type="entry name" value="Catalase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11465">
        <property type="entry name" value="CATALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11465:SF9">
        <property type="entry name" value="CATALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00199">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06628">
        <property type="entry name" value="Catalase-rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038928">
        <property type="entry name" value="Catalase_clade1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00067">
        <property type="entry name" value="CATALASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01060">
        <property type="entry name" value="Catalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56634">
        <property type="entry name" value="Heme-dependent catalase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51402">
        <property type="entry name" value="CATALASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0349">Heme</keyword>
    <keyword evidence="7" id="KW-0376">Hydrogen peroxide</keyword>
    <keyword evidence="6 9" id="KW-0408">Iron</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172683" description="Catalase core domain-containing protein" evidence="10">
        <location>
            <begin position="25"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="domain" description="Catalase core" evidence="11">
        <location>
            <begin position="57"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="9">
        <location>
            <position position="384"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR038928-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR038928-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP84867.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="527" mass="57927" checksum="98A60F0C3463CCCF" modified="2012-04-18" version="2">MLQSARILLSVAHLLPCIIQRSESSSLPNETTSSSQQLPGNLNASSFQIPSLSSILPSANSTKALNGLNGLASPAPIAPLSPDALAKQSEFDRKNIPARPVHAAGAGAYGTFTVTTNFAQSQTSMDLFSNVGQTTPVTVRFSNALGEKGSFDTARNVRGFAMKFQTKQGAWDLVSNNAPVFFIRDPAKFPPLIQSQSRDPLNNLGNADTAFNYFPNNPETMNMFLRIFSSAGASRGWIHTNAWSINTYRWYKSDGTWSYVRINFETKQGVLNFTASEQAQISDPSYGARELYSSIQAGQFPRWTMFAQVLSPKDAENFRYNVLDDTKEWPTSLVVPQEIGVLELNKNPANYFTEVEKLAFSPANVIPGWAASQDPVLQMRLFAYSDSSRYRLGSGNPTKMKKRFLQNAQTLNNPAQILKNSAQMLNNPTQIPNGSTTTAPKYDSGHSLWINQALKSLNQISPIDFEQPSFFYGNLTQDQKSELIQNIAGGLSVVSSLDIQTNLINWLAKASPDLSQQVSSKLKMMTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KL50</accession>
    <name>E3KL50_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11194</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <dbReference type="KEGG" id="pgr:PGTG_11194"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11194"/>
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    <dbReference type="InParanoid" id="E3KL50"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
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            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
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            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85025.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="79" mass="8701" checksum="31994872D25AF099" modified="2012-04-18" version="2">MGNTRKRHKDRASSTTSISSSPAQSKGPSHKKRNNAVVDTDGDYEVEREDTDVDHTTSASAQNLEKDQDQLDAEELTLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3KM06</accession>
    <name>E3KM06_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">TFIIS N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_11524</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Transcription factor involved in RNA polymerase II transcription regulation. May function in both SPT15/TBP post-recruitment and recruitment steps of transcription.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the IWS1 family.</text>
    </comment>
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        <property type="protein sequence ID" value="EFP85355.2"/>
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    <dbReference type="FunCoup" id="E3KM06">
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    <dbReference type="STRING" id="418459.E3KM06"/>
    <dbReference type="GeneID" id="10543898"/>
    <dbReference type="KEGG" id="pgr:PGTG_11524"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11524"/>
    <dbReference type="eggNOG" id="KOG1793">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_045275_2_0_1"/>
    <dbReference type="InParanoid" id="E3KM06"/>
    <dbReference type="OrthoDB" id="21124at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.930.10">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051037">
        <property type="entry name" value="RNAPII_TF_IWS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035441">
        <property type="entry name" value="TFIIS/LEDGF_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017923">
        <property type="entry name" value="TFIIS_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46010">
        <property type="entry name" value="PROTEIN IWS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46010:SF1">
        <property type="entry name" value="PROTEIN IWS1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08711">
        <property type="entry name" value="Med26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47676">
        <property type="entry name" value="Conserved domain common to transcription factors TFIIS, elongin A, CRSP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51319">
        <property type="entry name" value="TFIIS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TFIIS N-terminal" evidence="5">
        <location>
            <begin position="205"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
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    </feature>
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            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
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    </feature>
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    </feature>
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    </feature>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00037349"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00037992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP85355.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="373" mass="42211" checksum="A56418C8EC68A11D" modified="2012-04-18" version="2">MPRDLEDEIFGGESDLSDFDQDERPTEDVQENLAEEGRQHEEDDGGLSHDPAPREGSHTYPKFRKDTTQRSSEQSSAPKKKNQQRNKRPDSADNGVPPDDGEAEVVIDPEEQRRQELYQRIDEAAGKNKKGKRRRRKGGDEDLEMMADEEVSRLRTEMLSAADTDIQANEERRPATGKLMLLPKVKSTIQKSHLETAILDNGVLEAVKKWLEPLPDRSLPALNIQRELLELLSKMSIDTQSLKSSELGKIVLFYTKCPRVDPTIKRMADQLVTRWIKPILRRSARSREPIPMTAPRPVRPPTGGAPNSQRARIPETVHQVFHNPARSQLDSTTDGPGGSQNPAMRNSTQKATAKKTREWARKIQDARKIQRMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3KMB1</accession>
    <name>E3KMB1_PUCGT</name>
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        <recommendedName>
            <fullName evidence="1">[acyl-carrier-protein] S-malonyltransferase</fullName>
            <ecNumber evidence="1">2.3.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_11792</name>
    </gene>
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>holo-[ACP] + malonyl-CoA = malonyl-[ACP] + CoA</text>
            <dbReference type="Rhea" id="RHEA:41792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9623"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57384"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78449"/>
            <dbReference type="EC" id="2.3.1.39"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.1.39" evidence="1"/>
    <dbReference type="EMBL" id="DS178295">
        <property type="protein sequence ID" value="EFP85436.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329855.2">
        <property type="nucleotide sequence ID" value="XM_003329807.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KMB1"/>
    <dbReference type="STRING" id="418459.E3KMB1"/>
    <dbReference type="GeneID" id="10543987"/>
    <dbReference type="KEGG" id="pgr:PGTG_11792"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11792"/>
    <dbReference type="eggNOG" id="KOG2926">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021759_0_0_1"/>
    <dbReference type="InParanoid" id="E3KMB1"/>
    <dbReference type="OrthoDB" id="1929172at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004314">
        <property type="term" value="F:[acyl-carrier-protein] S-malonyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006633">
        <property type="term" value="P:fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.366.10">
        <property type="entry name" value="Malonyl-Coenzyme A Acyl Carrier Protein, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001227">
        <property type="entry name" value="Ac_transferase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014043">
        <property type="entry name" value="Acyl_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050858">
        <property type="entry name" value="Mal-CoA-ACP_Trans/PKS_FabD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016036">
        <property type="entry name" value="Malonyl_transacylase_ACP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42681">
        <property type="entry name" value="MALONYL-COA-ACYL CARRIER PROTEIN TRANSACYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42681:SF1">
        <property type="entry name" value="MALONYL-COA-ACYL CARRIER PROTEIN TRANSACYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00827">
        <property type="entry name" value="PKS_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55048">
        <property type="entry name" value="Probable ACP-binding domain of malonyl-CoA ACP transacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Malonyl-CoA:ACP transacylase (MAT)" evidence="6">
        <location>
            <begin position="170"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="14"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="107"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="337"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="496"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="61"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="115"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="497"/>
            <end position="506"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013258"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00048462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP85436.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="658" mass="72582" checksum="D198D5AD9136D7B9" modified="2012-04-18" version="2">MAQLIITQTRRHIAARLQQPKQQPQTSLRGLSRSTRNLGPKKPTTDKSSSTEEAEDEYHHYPTTHTTATTDSSAGLLQNPFLHDPAYIKRYRDPSRHDSYEIRDHHHPYSFRIRPTATQQSPSTSISSPARKARLSMEQSWLSPSTCHHHPSSHPDPLGSNGRPDNLAICFPGSSSQYIGMGSFLIDHKEFNLVWTEALDHLASFEEWMKSLNLVDHFLTLGYSPQQAALLGSPPPASPTLDQPNLRDIVLHGPQNELSRCSNAQPAILITSMAYLRTLEVEGKVPVRKMAKVYAGHSSGEYTACVASNAIDFKSGIHLTRLYGLLSERSMRLAGLSASTTAPKLKTTKEGEQEEGEEGGGGTKSQMSALLINEKYQASLGQKTYDYRELIGLVERFNMLYATDERRRSAYRVQIASFNSSNQIVLSGTRPGVLAACAYLNELEIANRAADLPCSSPFHSEFMSPAAQGMKVAVNAVRFAVPDRPILVTRNCPLSACSPTRSSSVGSTESTERRKRRKTDSGEAVMETAYISTREDLGSLQTHLAESICRPVWWAETVNELLLPHHNLLLHPSSNSESESEPDSSHSNLQLLFVGPGKALSNLCKKQIAFNALHPPLPSLPTHNLLSAQSSHPQAQPSHKVITRSLATVDDFNAFRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3KS03</accession>
    <name>E3KS03_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_13297</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EFP87078.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331497.2">
        <property type="nucleotide sequence ID" value="XM_003331449.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KS03"/>
    <dbReference type="FunCoup" id="E3KS03">
        <property type="interactions" value="161"/>
    </dbReference>
    <dbReference type="GeneID" id="10542320"/>
    <dbReference type="KEGG" id="pgr:PGTG_13297"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13297"/>
    <dbReference type="HOGENOM" id="CLU_152718_0_0_1"/>
    <dbReference type="InParanoid" id="E3KS03"/>
    <dbReference type="OrthoDB" id="6159137at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="25"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP87078.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="111" mass="11880" checksum="F3EE4A176CD9972D" modified="2012-04-18" version="2">MSSPVVVYEDDASYCSQHLIDPLSRIVSVINLALGTSEEDVRAAFCEFGKIQHCFVPLSAPTTAIVVFSTVAEARASTRSMDGAIADNKKIRVQRKGVAARGGLSLLLRIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KSM0</accession>
    <name>E3KSM0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178305">
        <property type="protein sequence ID" value="EFP87306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331725.1">
        <property type="nucleotide sequence ID" value="XM_003331677.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KSM0"/>
    <dbReference type="GeneID" id="10537706"/>
    <dbReference type="KEGG" id="pgr:PGTG_12890"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12890"/>
    <dbReference type="eggNOG" id="ENOG502SRMZ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034021_1_0_1"/>
    <dbReference type="InParanoid" id="E3KSM0"/>
    <dbReference type="OMA" id="EERIMWW"/>
    <dbReference type="OrthoDB" id="2588793at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="506" mass="58782" checksum="C4F41AB038056B97" modified="2011-01-11" version="1">MHQDQGRNTYVQIESQSEYELPSELQEPQNPKTCSSKMNNFFQAALWCLAGGTLIGFAVSVLWWEPVCQSPTSSASNSVPQTPVLNYNNTQSRVYSSTRVFLKKEEECNPFHEKGFLHYNASEPGDNTWRPYAPDCKPSNLFQKLKQDLHSTRETFGASEKDEFAWLRNRTVVLIGDSIDRWHNVDFCNVLSWEPGSTERDPIISENRPTKTYYVGADSPLSPPPWHFHPEDPLKPPEDWPEDEHELFKFQTPIWDAGQINANTTRPRVCEIPKYGFRVVSLFSWGLDPKEGGRLYANISGYFPPAEYTSRIDHILKPILENLATQSNNTLVTKPDLIEMSSGFWDLRQWAEEDKKIMEKRIGKPIDYPTEYGIGFSDLDQDRLNWWSERQKKAIRFVSDAFPQSQTPILWRSLHHTQTHHWVGYDRVNQIDQLARYNIEMLKLEDSSLEPRLRIDEWGALMLGQEHHFRDVMHVRELPGGVLWGDVMLWELRRAVKKRGSTYYSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KTW5</accession>
    <name>E3KTW5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_13454</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178308">
        <property type="protein sequence ID" value="EFP87668.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332087.2">
        <property type="nucleotide sequence ID" value="XM_003332039.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KTW5"/>
    <dbReference type="GeneID" id="10538900"/>
    <dbReference type="KEGG" id="pgr:PGTG_13454"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13454"/>
    <dbReference type="eggNOG" id="ENOG502T5HV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074882_0_0_1"/>
    <dbReference type="InParanoid" id="E3KTW5"/>
    <dbReference type="OrthoDB" id="2506589at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173891" evidence="2">
        <location>
            <begin position="20"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP87668.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="316" mass="33682" checksum="31596DBF5BEDC6AE" modified="2012-04-18" version="2">MISKIFLAYILMALGAVPASLVVANHTAAADTEVPGTLAHSTNATSSPNVPNAAHAATSPTSTTSENTPASSRHSPEGETTNGVTSVASDHAAEHHAELPPSTICHSKHKSHGVESHHCRKALDKVAFAANQTLDKFSGLIFANYKTCNVHIHKPMNATLQKAQLSLMVETLTTACHSVGGVLSQSSKFAMRIERSTKENVHEVNKSICKKEKCPLTQSDCLSAFYQLPTNAKGVFVNGKGKNPFARVTSGNCTVTASTTDLSAFTIPRQFVLPTMKKLLNECDEHPGKIYFGGGTKGYNGDIWLSARPANKDLCN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3KU34</accession>
    <name>E3KU34_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">NAD-dependent epimerase/dehydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_14524</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Mutarotase converts alpha-aldose to the beta-anomer. It is active on D-glucose, L-arabinose, D-xylose, D-galactose, maltose and lactose.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>UDP-alpha-D-glucose = UDP-alpha-D-galactose</text>
            <dbReference type="Rhea" id="RHEA:22168"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="ChEBI" id="CHEBI:66914"/>
            <dbReference type="EC" id="5.1.3.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate metabolism; galactose metabolism.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate metabolism; hexose metabolism.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">In the C-terminal section; belongs to the aldose epimerase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the N-terminal section; belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178309">
        <property type="protein sequence ID" value="EFP87809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332228.1">
        <property type="nucleotide sequence ID" value="XM_003332180.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KU34"/>
    <dbReference type="STRING" id="418459.E3KU34"/>
    <dbReference type="GeneID" id="10534707"/>
    <dbReference type="KEGG" id="pgr:PGTG_14524"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14524"/>
    <dbReference type="eggNOG" id="KOG1371">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_1_10_1"/>
    <dbReference type="InParanoid" id="E3KU34"/>
    <dbReference type="OMA" id="RDYDTPD"/>
    <dbReference type="OrthoDB" id="9402762at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003978">
        <property type="term" value="F:UDP-glucose 4-epimerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006012">
        <property type="term" value="P:galactose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005996">
        <property type="term" value="P:monosaccharide metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43725">
        <property type="entry name" value="UDP-GLUCOSE 4-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43725:SF47">
        <property type="entry name" value="UDP-GLUCOSE 4-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0299">Galactose metabolism</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="11">
        <location>
            <begin position="54"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP87809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="451" mass="49224" checksum="0AB41F9B33A46BFF" modified="2011-01-11" version="1">MNKCIKPSSTAPSRSRLLGRRIKKKQSSRLGLGRSGTKQQVVKRAVVVEDWPLILVTGANGYIGSHVVLELLKQGEYGVIAIDSLENSSAESLRRVRELASQEKSLGAHHPPIYHHQTDIRDRRGMLAIFQGYRQRDGRNAIRHVIHFAALKSVEGSRLNPAGYYSTNVLGTAGLLEVMKASGVNSLVFSSSAVVYGSRAGRPGGPGHSSTDLLAESRCPVRAHQPEAERQANYARITNTYGKTKLMCEELIHKLCYEHGQSTNSEENFRAVILRYTNPSGNDPSGRIGDSPTYPENLMPIAAQVLQGTREQISIYGADYPTRDGTGVRDFIHVQDLAKGHLAALSAFQPKGNKFSRGMYPNNCQTYNLGTGKGASVMEVIQALTEASGRPIKTTIAPRRPGDLATVICDPSKAELELGWKAEKGVLEMATDLWKFCVSNPHGLLPLKASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KUC6</accession>
    <name>E3KUC6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_14616</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178309">
        <property type="protein sequence ID" value="EFP87901.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332320.2">
        <property type="nucleotide sequence ID" value="XM_003332272.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KUC6"/>
    <dbReference type="STRING" id="418459.E3KUC6"/>
    <dbReference type="GeneID" id="10534880"/>
    <dbReference type="KEGG" id="pgr:PGTG_14616"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14616"/>
    <dbReference type="HOGENOM" id="CLU_082489_0_0_1"/>
    <dbReference type="InParanoid" id="E3KUC6"/>
    <dbReference type="OrthoDB" id="2150628at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051667">
        <property type="entry name" value="Archaeal_ATPase_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37096:SF1">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37096">
        <property type="entry name" value="YALI0E33429P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP87901.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="220" mass="25373" checksum="465839A70B930CA4" modified="2012-04-18" version="2">MTFLDFAVRMTTKEKKMHIIMTSSDSFFLQWIGKCINPTYLDWFVLGDMTRDEAHRYFLHALETDCRLSEEKKAMLGSVDSDTIYRLTGGRPIFIESYIRQVHQSGFFVDPLRFQPVRQAYGCMFNSLGDEPKTYGKAETLAVSSLLVNSPGHHTSYGNLAIKLGLPVVEEMFERNFLQYRPPSTFSRDLDPSPYETVVTAQSQPCLRAMEWFVNSHRNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVA8</accession>
    <name>E3KVA8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Suppressor of G2 allele of SKP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_14287</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178312">
        <property type="protein sequence ID" value="EFP88203.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332622.2">
        <property type="nucleotide sequence ID" value="XM_003332574.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KVA8"/>
    <dbReference type="FunCoup" id="E3KVA8">
        <property type="interactions" value="540"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KVA8"/>
    <dbReference type="GeneID" id="10535511"/>
    <dbReference type="KEGG" id="pgr:PGTG_14287"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14287"/>
    <dbReference type="eggNOG" id="KOG1309">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039532_2_0_1"/>
    <dbReference type="InParanoid" id="E3KVA8"/>
    <dbReference type="OrthoDB" id="1898560at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06466">
        <property type="entry name" value="p23_CS_SGT1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000012">
        <property type="entry name" value="SGT1 homolog, MIS12 kinetochore complex assembly cochaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007699">
        <property type="entry name" value="SGS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044563">
        <property type="entry name" value="Sgt1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45862">
        <property type="entry name" value="PROTEIN SGT1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05002">
        <property type="entry name" value="SGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51048">
        <property type="entry name" value="SGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="3">
        <location>
            <begin position="14"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="SGS" evidence="2">
        <location>
            <begin position="132"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP88203.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="230" mass="25903" checksum="DAEB226D5805FD72" modified="2012-04-18" version="2">MTQTATDSPDPQSQPRIRHEWYQTDVEVVLSVFVKNTKSEDINCDFGPRSISLNYKLPTNGSEGCFDLDPLSYEIEPSQCSWKSLPSKIDIRLKKKVPGIKWLVIEGDQADLPAPTILQESSTATDVTRQPAYPSSARRKTNWDQLANSVEKEEEEVIKNLKDPNAGGDRAVNELFQKLYADATDEQKKAMMKSYVESNGTALSTDWSDVSKKKVETRPPDSMVAKTWKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVJ0</accession>
    <name>E3KVJ0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_14414</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <source>
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        <source>
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    </evidence>
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        <source>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
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    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
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                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
            <dbReference type="EMBL" id="EFP88715.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
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                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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            <strain>CRL 75-36-700-3</strain>
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    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
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        </source>
    </evidence>
    <sequence length="409" mass="47459" checksum="C2A97AE9B87A6575" modified="2012-04-18" version="2">MLFVQLFIALHFIQPYGVFAHTVPTFVKPLKEVFPASKPLGKLDTAFRDCYNLDGGRFHSPPRDQFPPILENLHSTGIPKRQNRDPKRRVTWDESPVKVFEIPKEEPEPGEEIPQYVKRYVQKRWQWRHIDDFTDDERKRKYGTRYLIGSNRQIHEYTEFPTKKVNFKGNFIRVPADFPPPSEEKILPPEFQEGNLERKLSPTEYRNSFEEEKDEGKAGGEELNNSGSYPMINEHSVNKKETDINENQEFDEDAKHRKAGYKKVSTSFRDQIIGRHKETHISETKKFDDPNQGKAGSEKLSTSVTDQIDTSYSPKIKSFKIGNDPLGEEKFSENKNGGLKEKEIQDPEPKYAKFQNPEDLKHKKAGYKKVSTSFRDQIIGRQKETHISETKKFDGVKALMTKSLNIYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>E3KYE9</accession>
    <name>E3KYE9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Vacuolar transporter chaperone complex subunit 4</fullName>
            <ecNumber evidence="3">2.7.4.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Polyphosphate kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">SPX-dependent polyphosphate polymerase VTC subunit 4</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Vacuolar membrane polyphosphate polymerase catalytic subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PGTG_15519</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>[phosphate](n) + ATP = [phosphate](n+1) + ADP</text>
            <dbReference type="Rhea" id="RHEA:19573"/>
            <dbReference type="Rhea" id="RHEA-COMP:9859"/>
            <dbReference type="Rhea" id="RHEA-COMP:14280"/>
            <dbReference type="ChEBI" id="CHEBI:16838"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:19574"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Vacuole membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the VTC4 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.1" evidence="3"/>
    <dbReference type="EMBL" id="DS178321">
        <property type="protein sequence ID" value="EFP89340.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333759.2">
        <property type="nucleotide sequence ID" value="XM_003333711.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KYE9"/>
    <dbReference type="SMR" id="E3KYE9"/>
    <dbReference type="FunCoup" id="E3KYE9">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KYE9"/>
    <dbReference type="GeneID" id="10547062"/>
    <dbReference type="KEGG" id="pgr:PGTG_15519"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15519"/>
    <dbReference type="eggNOG" id="KOG1161">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4580">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009308_0_1_1"/>
    <dbReference type="InParanoid" id="E3KYE9"/>
    <dbReference type="OrthoDB" id="6493944at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033254">
        <property type="term" value="C:vacuolar transporter chaperone complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008976">
        <property type="term" value="F:polyphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006799">
        <property type="term" value="P:polyphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07751">
        <property type="entry name" value="PolyPPase_VTC4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14480">
        <property type="entry name" value="SPX_VTC2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.100.30:FF:000001">
        <property type="entry name" value="Vacuolar transporter chaperone 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.100.30">
        <property type="entry name" value="VTC, catalytic tunnel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051572">
        <property type="entry name" value="VTC_Complex_Subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018966">
        <property type="entry name" value="VTC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042267">
        <property type="entry name" value="VTC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46140:SF1">
        <property type="entry name" value="VACUOLAR TRANSPORTER CHAPERONE COMPLEX SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09359">
        <property type="entry name" value="VTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="789"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="821"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="860"/>
            <end position="877"/>
        </location>
    </feature>
    <feature type="domain" description="SPX" evidence="18">
        <location>
            <begin position="1"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="85"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="529"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="690"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="58"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="96"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="544"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="16">
        <location>
            <begin position="715"/>
            <end position="726"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00075894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00080494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00081313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EFP89340.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="893" mass="101196" checksum="B5F17B91C8AB58C5" modified="2012-04-18" version="2">MKFGVTIKDALHEEWSKYYVDYAGLKKFIKSRQAKKQWDENDEQVFVRELDQELQKVANFQERKISDLHQKISLYEAEVKELIESNSGRRSKPASNEDENEEPPTDEERIEATLGSTSDDELDEEAEEHYATLEYELTHIIADVHDLGHFSHLNYTAFIKIVKKHDKKTGWELRREFIQHHLETRPFYKENYEALVVQLSRLFNLVRTRGQPPVGDSAAGGGQSAFVRQTTKYWVHRDNFVALKLLVLKHLPVLVFNPDKAFEPSDSAISSIYYDNEDLELYLGRLEKTEGAEAIRMRWYGDMSVKQIFVERKTHREDWTGEKSVKARFSIKEEQLNDYLAGKITMDEHFEAIRKKGKKSDKEVDSMIRLAKEVQERVRQKKLQPCVRTFYNRTAFQQPGDARVRISFDTDLTMVREDNWDGKTRRPDNNWRRTDAGIDFPFPTVEEDDKHIFPYGVLEVKLQTQLGQEPPEWVKELVSGPLVEAVPKFSKFIHGCATLMPNRVSLVPFWLPQMEIDIKKNEPLKLIDRPATLSRHPSEIRPSGNRNGKSNADQARPYSEPLSEDEEGIEGSGDRLQKASSPETEGQWAGLSADVATSATASRVAGANPDGAPSSTGNIVEEAKGLAPPGSSPEQLEGAMEVDLEANDNPSGTEQRPSEPAYFAKLTPGNLRKMLKAKAKMLNQSDRIFKNLRGPSQAVDSHPSRSLPSDRNPADEDDDEDDDEDDQGPKVVGATAPRSAAVHPNLPHSSSASRNAVEWIRYFNAPKGKKIAIPIRIEPKVYFATERTFFTWLEFSTILSGISLSLLNFSPINDRTGFRISVIFTILALLSIVYSTLIFVKRVHKIRTRQASGYYDPVGPSVLCACILIAVAVNFYLRFSDQNGLPIPSTPSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZR3</accession>
    <name>E3KZR3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_15444</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178325">
        <property type="protein sequence ID" value="EFP89788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334207.1">
        <property type="nucleotide sequence ID" value="XM_003334159.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZR3"/>
    <dbReference type="STRING" id="418459.E3KZR3"/>
    <dbReference type="GeneID" id="10545439"/>
    <dbReference type="KEGG" id="pgr:PGTG_15444"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15444"/>
    <dbReference type="HOGENOM" id="CLU_819240_0_0_1"/>
    <dbReference type="InParanoid" id="E3KZR3"/>
    <dbReference type="OrthoDB" id="10345842at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174272" evidence="2">
        <location>
            <begin position="25"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="225"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP89788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="340" mass="34560" checksum="C9CE5C176BC75364" modified="2011-01-11" version="1">MISSRFIQLVAIVFCAYACLDIQAQAVTGPSKPSQPSSNAPPHLGMTGDNKTNNPNGLHTAPRQSQAPSVPSSGAKQPAQNPSKTMNHSQTKTAEMAKPTTGLEDAKPVGPGPVGTEKAESGEEKIPAGAEGLTKRGGLRDVLWKRGDLPSIQGIGKKPTSLKEHNPTKKPDEGPGTNTGGGKTATGQSTMARESSGYVAGSSAPDAGKNKSGRNKLMLNGGSGSSEPSDTSPSSEGNAEKNKQGGVHASLNERDGESIQHLLRKRQDAGFYKTKFKAPKLGGGTTSGVAVGTFGGPPVPINGVMGPTGPIPLPTAIVPVTPVGGNGMGGMPPMLGGGYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3L062</accession>
    <name>E3L062_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16237</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178327">
        <property type="protein sequence ID" value="EFP89949.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334368.2">
        <property type="nucleotide sequence ID" value="XM_003334320.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L062"/>
    <dbReference type="STRING" id="418459.E3L062"/>
    <dbReference type="GeneID" id="10545764"/>
    <dbReference type="KEGG" id="pgr:PGTG_16237"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16237"/>
    <dbReference type="eggNOG" id="KOG1426">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017519_1_0_1"/>
    <dbReference type="InParanoid" id="E3L062"/>
    <dbReference type="OrthoDB" id="61110at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.30">
        <property type="entry name" value="Regulator of chromosome condensation 1/beta-lactamase-inhibitor protein II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036047">
        <property type="entry name" value="F-box-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051553">
        <property type="entry name" value="Ran_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009091">
        <property type="entry name" value="RCC1/BLIP-II"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982:SF3">
        <property type="entry name" value="F-BOX PROTEIN POF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45982">
        <property type="entry name" value="REGULATOR OF CHROMOSOME CONDENSATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13540">
        <property type="entry name" value="RCC1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81383">
        <property type="entry name" value="F-box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50985">
        <property type="entry name" value="RCC1/BLIP-II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="529"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="605"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="655"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="529"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="606"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="662"/>
            <end position="672"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89949.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="681" mass="76069" checksum="C589B2B16F9780C2" modified="2012-04-18" version="2">MAPHGTTGLISSLPLDLLIDSILVLLPTRDLIALSCTCRLLHSTIHQTELIWKRKIAIDYRFPTGSTGRITGFKTLYRKLRKPQVWVWGQNMNDRLGLNGEDEEDLLEAVLSHNVEVPTPLRLRKAESIGIIDLQAGGWSYHGLDVNGNVWCWGTLNGSDGWNPPPHRYSPKPSEVEPTMLEIKNLPKFKAISCGRSHACGISDEGGIYEWHSWNRVAKLTTVPWETHAGVTEEVAQICASWNFTALLSSDGVIYFWKEPTILEVAQALDSQFNGLPEHDTMETVYDLDSKDRIIRCPDLPGNDHDKDQITKIACGEDFIICLTTTGKIYKLDLSRIADTQRTAPQRVLRQLQQHDEELDYRESIAQAFRTNEREWEYLPMFCEPDKLRDGLANALTLQDSSNAAQPTITHISAQYRTFVAYSVESPSENEPIIKNNSFVFFGGKETTENSKPEILPNLQNRGVIQVTLGDYHYAALLSNGQILTWGSYSGGALGLGNSSNPHRQLCHDDSAAESPGDPVKERYELGTETPTVVQSFSGTTHHLADRIGTGDSSSSQKPRKVARRYVFSVTAAGWHTGCLAFSLDDEDDEDYGLAVLEKDVEFKQASRAAGSSSRQDQQDGGRTSWYGLPFINRTRFPSFRIGFAGRFMQGGARGGLLNHQGFRDQRERAEQDPPPTEEQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3L0M4</accession>
    <name>E3L0M4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178329">
        <property type="protein sequence ID" value="EFP90152.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334571.2">
        <property type="nucleotide sequence ID" value="XM_003334523.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L0M4"/>
    <dbReference type="GeneID" id="10546211"/>
    <dbReference type="KEGG" id="pgr:PGTG_16000"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16000"/>
    <dbReference type="HOGENOM" id="CLU_005726_6_0_1"/>
    <dbReference type="InParanoid" id="E3L0M4"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35871:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35871">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="586"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="595"/>
            <end position="605"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90152.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="815" mass="91927" checksum="C688B772A35401E5" modified="2012-04-18" version="2">MAKSIKASKAQIKRWAEEKNDPTRTRKKARHSQVDPIQIESSSDNENPSDQPLGTSNQPISVPDDNNALAEDGLINRFQEREIEEVDEAHDMIQFLHESLDDPTSDEGEDLDGNSDSDTEDTPSNFWPLFFGKNKPSITTSQRKLKSGRTGYQKPVSNPLSTSGKLVPRPLPRQTKHDYHKNFIKAVGKGNMMMENFITRTPGVPRPAASLASSTNLTPIPELPPIQSPNSDPTATNQNLVELRLELHFNRYHSAPQKKVVNKDEKLKSKAKHRWQQLNSAIVSAIKLSKSAIRRQLVSSSSINEPPPSSSGLNQARIISKQARHVLAHKEFLETQLGNKTVHQSLLNNPEVRKALFAWAAAQTPGEVTPATLHHHVNHTILPKCKIERTVTIKSVTQWMYKLGFSRQEYRKSQYFDGHERPDVVLSRKKYVDDYLLLRQRSRKYGGENFEISTEIDPEILGHMKETIFIFHDESTIHAKERPSKTWLLPGTSELRSKSSGRLIHISDFILESTGRLVLSKDELEKLKTKQPTGGVFVFDCSSAHGAFSPSALRVQKMNLGHGGKQARLRDTVIPSDDPLIPLHLHGQPQTLSYDDSHPDPERAGKQKGVKAVLEERGLWQHYTRQSQESGKPALTLSCKTCTTSNIWKDAISRAERLIQQAEDCGYFLSNAQSMSEIAETEAAKNQESEESTLDPVPNDAGSMCCWSRILSLQSDFANERPLLQTIIEDRGHICLFLPKISLRIKSNRTFLVIHQAGIPKEVAYNMKYFCRTDRQVNVYQQGYNGAQSQILMKKYKSHRCIPRNAAMRIDMLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3L1S7</accession>
    <name>E3L1S7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_16089</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178333">
        <property type="protein sequence ID" value="EFP90502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334921.1">
        <property type="nucleotide sequence ID" value="XM_003334873.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L1S7"/>
    <dbReference type="GeneID" id="10530399"/>
    <dbReference type="KEGG" id="pgr:PGTG_16089"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16089"/>
    <dbReference type="HOGENOM" id="CLU_026798_3_0_1"/>
    <dbReference type="InParanoid" id="E3L1S7"/>
    <dbReference type="OrthoDB" id="2508544at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="236"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="96"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP90502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="511" mass="58074" checksum="14A762E4F92B3549" modified="2011-01-11" version="1">MTKHNLNQAANSSELVSDLNPGNMIHRRSLSDLASTLTELSISSDSDDQPIASLKDQVSHEGTVAGQAPNRQTTSKKHLKTVVKKESKIKSSYQKSYRKQKSQGHRPQRRRNACVKAIQSHLANLPLQGPFVISRRTPKPFVLYPEVTQDFEIRKEERRIAQENFEKKIGPQPPATTIYERKPTPEEIKQAYDRVHDSFDLYDHGHVTVFDNSNPKVKDKIIAAIHFTDLTKLTPLQIGDINFLCKFLQDSKRFVNPVSSPSRLCAGKMWAIGWRKSMTNLEIVGMYRNWDAIKKNKKDYRLFLGDAERAGRIVWDLFHPVGNIALEKNQQFMIEHNIPGFYEAEFPNDKSKPSKEFFSSNLTFTSNGFFNHPHEDKNDHASLPFAFLLSLPISKETGLLAYGADEYDVTEGPFIFRDCQFGIKFRPNTMCQMIFSQREYSHCTLAPSEPSAFTRLGLSLQISAKMVNISDRITGGEFDNRKDMHIAGAEYNLNVTINKSKAPVQSSQLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3L2J2</accession>
    <name>E3L2J2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16851</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178337">
        <property type="protein sequence ID" value="EFP90825.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335244.2">
        <property type="nucleotide sequence ID" value="XM_003335196.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L2J2"/>
    <dbReference type="STRING" id="418459.E3L2J2"/>
    <dbReference type="GeneID" id="10530729"/>
    <dbReference type="KEGG" id="pgr:PGTG_16851"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16851"/>
    <dbReference type="HOGENOM" id="CLU_031560_1_0_1"/>
    <dbReference type="InParanoid" id="E3L2J2"/>
    <dbReference type="OrthoDB" id="2506424at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90825.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="353" mass="39138" checksum="D82D16781E1C7590" modified="2012-04-18" version="2">MPPNDDDAHHHNREIEMTSYEANDSNNNPISASTSNNPPLNKSRHNPDNQGVLKIISNLIMNPEADGSVIITPDKVQLLKSLIGVEEFINVKTLTMMDKITSRLDAIEKSISKSTPTTKTPTANWSTIVKKSHPVPNTTIARAPPSVRVINEFKPSFFTIRKTVPEAQAFAQKSPAQIIEKVNSVLSNMEAKTEDGTPITIKGAISLPLGDIKFFTPTRFATNWLLEHKHEWTHLCDPALVTPQTSFPVIIHTVPISFTPTNKATIADLCRENNGMDSHSIQSMRWLGNPQANKKAHGSIIMNLLDKEIVKKIEKGGLFFKGLYFKGAHYKKSPMQCSQCLEVGHTAQVLYSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>E3L302</accession>
    <name>E3L302_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_17199</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178339">
        <property type="protein sequence ID" value="EFP90927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335346.1">
        <property type="nucleotide sequence ID" value="XM_003335298.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L302"/>
    <dbReference type="GeneID" id="10528341"/>
    <dbReference type="KEGG" id="pgr:PGTG_17199"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17199"/>
    <dbReference type="HOGENOM" id="CLU_2795170_0_0_1"/>
    <dbReference type="InParanoid" id="E3L302"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP90927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="68" mass="7767" checksum="7EE735FD55FFE55F" modified="2011-01-11" version="1">MEIRSVLFPSAPDTVDMGVDALFCFERERGMRWDVWPPTSFRSLIFFLRGAVLGGWIIRGDRFVDNGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3M8</accession>
    <name>E3L3M8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Trafficking protein particle complex subunit 2-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_16898</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAPP small subunits family. Sedlin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178342">
        <property type="protein sequence ID" value="EFP91153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335572.1">
        <property type="nucleotide sequence ID" value="XM_003335524.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L3M8"/>
    <dbReference type="FunCoup" id="E3L3M8">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L3M8"/>
    <dbReference type="GeneID" id="10528815"/>
    <dbReference type="KEGG" id="pgr:PGTG_16898"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16898"/>
    <dbReference type="eggNOG" id="KOG3444">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085828_2_3_1"/>
    <dbReference type="InParanoid" id="E3L3M8"/>
    <dbReference type="OMA" id="FHYIVHC"/>
    <dbReference type="OrthoDB" id="18320at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14854">
        <property type="entry name" value="TRAPPC2L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006722">
        <property type="entry name" value="Sedlin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044760">
        <property type="entry name" value="TRAPPC2L"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12403">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04628">
        <property type="entry name" value="Sedlin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP91153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="155" mass="17447" checksum="34DB54DED8F481A9" modified="2011-01-11" version="1">MSSNTGRLPILALAIIGKKGQPLYVRNFASPSGGEADLKWHYAAHVSLDVFDERDALPTTLVDCYFGLLYAMEDYACYGYQSNTRIRFVLCLPMKDTLIKDTEVKLLFKSLHAAYIQHICNPFHQIPIDNPNPNAITPTIKSKTFDQQINRIVGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3X2</accession>
    <name>E3L3X2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_17104</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178344">
        <property type="protein sequence ID" value="EFP91247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335666.1">
        <property type="nucleotide sequence ID" value="XM_003335618.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L3X2"/>
    <dbReference type="GeneID" id="10529150"/>
    <dbReference type="KEGG" id="pgr:PGTG_17104"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17104"/>
    <dbReference type="HOGENOM" id="CLU_028357_0_0_1"/>
    <dbReference type="InParanoid" id="E3L3X2"/>
    <dbReference type="OrthoDB" id="2499850at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37471:SF1">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37471">
        <property type="entry name" value="UNNAMED PRODUCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="73"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="318"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP91247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="361" mass="41015" checksum="2A45CF083B9F191B" modified="2011-01-11" version="1">MSFIFNAPALAVDVGITLIYGFIGGCWLVVIYRMVSLGGQLALLSLPSSFPHYPSFSSASTAQEYWISLGGEFLFAISLLEVIFSIYCLYLIRCAQALPDSTPRSLELLKDLIVHALGSGLEPDPPSDPHTRTTDEKDLDPDLGIAPSTAFLNKPLPFDHPKAKDFRENHSIWFQNSRWEDIYRENHLEWLSAALLNKPLEKVKEEDKLKSKEEAVLPLLDELVCAYEKRVGTRLPDGYNEYLADKTIMLFKDPIRVSLRPLTLSYGVAWSVNEIIRQLLRYKGFKLKCCSNRKNGLKYFIRIPDSWRKLPSDQRPPAILFIHGIGTGFLLYSSLIKYLALSPWANERPVMILVQPHISWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3L458</accession>
    <name>E3L458_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16987</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178345">
        <property type="protein sequence ID" value="EFP91333.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335752.2">
        <property type="nucleotide sequence ID" value="XM_003335704.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L458"/>
    <dbReference type="GeneID" id="10529163"/>
    <dbReference type="KEGG" id="pgr:PGTG_16987"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16987"/>
    <dbReference type="HOGENOM" id="CLU_399622_0_0_1"/>
    <dbReference type="InParanoid" id="E3L458"/>
    <dbReference type="OrthoDB" id="2498862at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91333.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="672" mass="75523" checksum="E8F828032598425A" modified="2012-04-18" version="2">MIDTSDGALPRTEDAGHSAELLQRTDQEGFRAEVYWLPSFNRRRANGISESHSASPPAPPDSKSKRPVNEGRFRNLFKRLAFDCWGGPSGTLTDPALGPVILFEERYAEIVGRKRDDERLELVDEAFRYILLSISMSRTEAVGWVSIFSGLLAHYRGSQRLPTSLVYGLHSLLLKHLSDPTLSSSICDLLLEILTTINPRVRPLLIAKWSQGNLEKLVKIISAEPTLSVLLAGLQAKRVDEILGGFGVIGDIFSARAPGYFSSQTRVASIAVLFHLIGMGIPKITEEAISISKALLVNDEEYFLLGHERALLDKILSDYKEATSDREHTNILTGSQIFTVQSQELFLRTTEKGIWSKPYWFPGFDQFAELSLAEQMEDFHARLGGSGQTADRQSVIDTIEPESRRRAWGSQLSLNPSEEIPAGSIASNGELGVLKFFKERCQDLQRTSCPNEALLRLVQRALLYILQLPKLSSTSLKGWLTQLKALAINTSRDHELLTTIFYGFYSIHLKLSHDNQLENPVRPFLDLLISSIPINILREVQGEWSRELFNPTLGLSPKFRDWKELNKIIVNEPTLEKLRMCLNHQEHERIEQHMSSLERIFSGPESVGFSAETRVASIALLAHLIAIPEPRLAEFPLGLSTWLLAETRLLFPHERLLLASILQDVNHLTRSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3L4W6</accession>
    <name>E3L4W6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">succinate-semialdehyde dehydrogenase [NAD(P)(+)]</fullName>
            <ecNumber evidence="6">1.2.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_17645</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>succinate semialdehyde + NAD(+) + H2O = succinate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13217"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>succinate semialdehyde + NADP(+) + H2O = succinate + NADPH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13213"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; 4-aminobutanoate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.16" evidence="6"/>
    <dbReference type="EMBL" id="DS178349">
        <property type="protein sequence ID" value="EFP91591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336010.1">
        <property type="nucleotide sequence ID" value="XM_003335962.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L4W6"/>
    <dbReference type="FunCoup" id="E3L4W6">
        <property type="interactions" value="154"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L4W6"/>
    <dbReference type="GeneID" id="10531528"/>
    <dbReference type="KEGG" id="pgr:PGTG_17645"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17645"/>
    <dbReference type="eggNOG" id="KOG2451">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_1_0_1"/>
    <dbReference type="InParanoid" id="E3L4W6"/>
    <dbReference type="OMA" id="IGELFCK"/>
    <dbReference type="OrthoDB" id="310895at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004777">
        <property type="term" value="F:succinate-semialdehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07103">
        <property type="entry name" value="ALDH_F5_SSADH_GabD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000004">
        <property type="entry name" value="Succinate-semialdehyde dehydrogenase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000005">
        <property type="entry name" value="Succinate-semialdehyde dehydrogenase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050740">
        <property type="entry name" value="Aldehyde_DH_Superfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353:SF10">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE (NADP+)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43353">
        <property type="entry name" value="SUCCINATE-SEMIALDEHYDE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="9">
        <location>
            <begin position="54"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00050387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00052698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP91591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="519" mass="55841" checksum="220255512E524725" modified="2011-01-11" version="1">MLTNKLIRPSVSIHPINRSSASFSTYPINRSSVLSAKLKNHDLLKHQSYINGQWVGSSTSRTFAVKDPATGDEIGNCPDMNLVDLKLAIDAAKKSFSSYRYSTPASRQSYLRRFNQLILSNAEDLARLIVAENGKCWKDAMGEVNYAASFVDWFAEEALRTDGTIVPSSTPGIRHLVIKQPIGVVAALCPWNFPAAMITRKVAPALAAGCSVIIKAPAETPLTALALAELAHQAEFPAGVFNIVTTQSATPEIGKALCESEEIRKLSFTGSTGVGKLLMSQSSSTLKKLSLELGGNAPFIVFEDADVDRAVEGAIACKFRCAGQACVSANRFYVHKSIYAEFASKLTRKVSELKIGSGFEEGVLIGPLIDERSLEKVSRHVRLAKEAGAEVLIGGKKVDGVGHFFEPTVLSEVPSGVIDDEETFGPVAALYKFESESDVILQANKSEVGLAGYFYSRDIGRIWRVAEALEVGMVGANTGMVSNAVTPFGGIKHSGFGREGSKFGIEEFQIVKYIAMGGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>E3L562</accession>
    <name>E3L562_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3-hydroxyisobutyrate dehydrogenase</fullName>
            <ecNumber evidence="3">1.1.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_17543</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>3-hydroxy-2-methylpropanoate + NAD(+) = 2-methyl-3-oxopropanoate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17681"/>
            <dbReference type="ChEBI" id="CHEBI:11805"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57700"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid degradation; L-valine degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HIBADH-related family. 3-hydroxyisobutyrate dehydrogenase subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.31" evidence="3"/>
    <dbReference type="EMBL" id="DS178350">
        <property type="protein sequence ID" value="EFP91687.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336106.2">
        <property type="nucleotide sequence ID" value="XM_003336058.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L562"/>
    <dbReference type="STRING" id="418459.E3L562"/>
    <dbReference type="GeneID" id="10531626"/>
    <dbReference type="KEGG" id="pgr:PGTG_17543"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17543"/>
    <dbReference type="eggNOG" id="KOG0409">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035117_6_1_1"/>
    <dbReference type="InParanoid" id="E3L562"/>
    <dbReference type="OrthoDB" id="435038at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008442">
        <property type="term" value="F:3-hydroxyisobutyrate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006574">
        <property type="term" value="P:L-valine catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1040.10:FF:000006">
        <property type="entry name" value="3-hydroxyisobutyrate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000746">
        <property type="entry name" value="3-hydroxyisobutyrate dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1040.10">
        <property type="entry name" value="N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002204">
        <property type="entry name" value="3-OH-isobutyrate_DH-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013328">
        <property type="entry name" value="6PGD_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006115">
        <property type="entry name" value="6PGDH_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029154">
        <property type="entry name" value="HIBADH-like_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22981:SF7">
        <property type="entry name" value="3-HYDROXYISOBUTYRATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22981">
        <property type="entry name" value="3-HYDROXYISOBUTYRATE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14833">
        <property type="entry name" value="NAD_binding_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03446">
        <property type="entry name" value="NAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00895">
        <property type="entry name" value="3_HYDROXYISOBUT_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0101">Branched-chain amino acid catabolism</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="6-phosphogluconate dehydrogenase NADP-binding" evidence="8">
        <location>
            <begin position="66"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="3-hydroxyisobutyrate dehydrogenase-like NAD-binding" evidence="9">
        <location>
            <begin position="250"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP91687.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="389" mass="41906" checksum="A02B74D8D41E9BF9" modified="2012-04-18" version="2">MEIKDEGLIIRSSSKRMASINYRTMIRSRGYKIGTLAARRLRIVEQRYGFSTTGVHRMAEHRRENSIGFIGLGAMGQEMASNLLSKFPSPISAFVIHDHSQSSVARFVDRHPSLNADSTTRIVTATTPASVARQASTIVTMLPSSAEVSAVYLDPNTGILRGINEEEHSSSATLCIDSTTLDVGCATTVSEKLRAAGAVEMIDAPVSGGVVGAREGTLTFMCGGPEETFNKAQPFLNAMGKKTIYCGKSGSGLAAKLTNNMLLAVSMIATSEAMLLGQRLGLKPDLLARVINSSSGRCWSSEINNPAPSALLPSKRTPADDDWQAGFASRLMAKDLGLALQAEPALLPEHLPVSNLVKEIYLRLTSDPAFSNKDFSVVYDWLQNNHSQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3L649</accession>
    <name>E3L649_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">FAS1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_17855</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178356">
        <property type="protein sequence ID" value="EFP92024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336443.1">
        <property type="nucleotide sequence ID" value="XM_003336395.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L649"/>
    <dbReference type="FunCoup" id="E3L649">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L649"/>
    <dbReference type="GeneID" id="10531978"/>
    <dbReference type="KEGG" id="pgr:PGTG_17855"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17855"/>
    <dbReference type="eggNOG" id="KOG1437">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003373_0_0_1"/>
    <dbReference type="InParanoid" id="E3L649"/>
    <dbReference type="OMA" id="PPYPPWM"/>
    <dbReference type="OrthoDB" id="14252at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000077">
        <property type="entry name" value="Chromosome 1, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.180.10:FF:000062">
        <property type="entry name" value="Vacuolar membrane protein, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.180.10">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050904">
        <property type="entry name" value="Adhesion/Biosynth-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036378">
        <property type="entry name" value="FAS1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000782">
        <property type="entry name" value="FAS1_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10900:SF77">
        <property type="entry name" value="FI19380P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10900">
        <property type="entry name" value="PERIOSTIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02469">
        <property type="entry name" value="Fasciclin"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00554">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82153">
        <property type="entry name" value="FAS1 domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50213">
        <property type="entry name" value="FAS1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174060" description="FAS1 domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="994"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="943"/>
            <end position="963"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="4">
        <location>
            <begin position="37"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="4">
        <location>
            <begin position="202"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="4">
        <location>
            <begin position="358"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="domain" description="FAS1" evidence="4">
        <location>
            <begin position="521"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="708"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="831"/>
            <end position="852"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP92024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="994" mass="109786" checksum="91B06C70DF5EAB28" modified="2011-01-11" version="1">MVNRILLLLLPLLPLLISAEHHQQQQTSFELPRTSTSTTLIDLLSNSTEHTKLLHLIQISRLVPTINSLSSASLFAPTNQAIESAGLSSLSTTQDNLQLELRQTLLYHLLNYTLPDSTGPSDSPQLLDSLLFPEPINTGNTDDRRLLLGHQPQKLRIIQRDQDRFIGVDQRGQGGIRLPPPDQFQNATNGILIPIQSILKPPRSLKHLIKSTPTLSTYASILPDRLLDQLDLLPHLTIFAPQNQAWDHLNSIELGYLRSNFSADDSIKLFRQATADNVLGSEGIGYSDLLRREALKHQPAQSFVLFSVDGKPLSIGIDPKSNLPSINGSEIIENDILASNGVLHIVPNLLISSGDNPLQLTPEKYLLALNCTKFVSLFRQANLSSTYLDNRSSDKSYTILAVRDDVLRSTSSSSSLGRSSIDRLKTNDTESLKKSLRYHVIEGNYLIDDLEDGMLLRTELKLQGGSSSNKQRMPVSVSTNDRKGLSKSPIIGFGGANVVGGDPIQVGNTVIYILSQIVEPPSDLLQVALSDIRFSTGVASIFSAKLDHQLQALPELTYLMPTNKAFANLGLIMDYLLLEKSKPDLLQVLKYHAVTEVIYLNELKIGSSQRYPTLEGTELYISKLATNNVTLHGPTVGGVALNGEDKDSEVINKRDVLVENGSMQFISQVELPPGVNIDLRKLMVGAKATTMLELIQATNLTWILDSGVPPSSSKDDRSSSGQKKNKNKEDQINLSRGYTILCPSDSAFTKINLTYFLEDPIRLESLVRQHIIPVYPDESFPGTGDSLDHTHQPPALFEPLYLEDGLVYPTLLSTKEGGPSRFGDLSFRFNRPQEVNNHGDHRGGDDDDDDNDRNKFFGKWRVGIKGARGSDQSHDSARILNYGRATPKIISSSSSSWNSSFIDSSLRISHPDSISFAGGVLAIDMVLLPYYPSFWNRIGKFIFLFFFLTLIAVAIGFGGWKIYQIKYRRNNYAAVRVGPGGGIGGYQVVEPMEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3L692</accession>
    <name>E3L692_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GCM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18157</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178357">
        <property type="protein sequence ID" value="EFP92067.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336486.2">
        <property type="nucleotide sequence ID" value="XM_003336438.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L692"/>
    <dbReference type="GeneID" id="10532024"/>
    <dbReference type="KEGG" id="pgr:PGTG_18157"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18157"/>
    <dbReference type="HOGENOM" id="CLU_005992_0_1_1"/>
    <dbReference type="InParanoid" id="E3L692"/>
    <dbReference type="OrthoDB" id="2624269at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="527"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="605"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1127"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1157"/>
            <end position="1167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92067.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1167" mass="131427" checksum="F28AE0D2DCEB3D51" modified="2012-04-18" version="2">MDPPRFGEDAKWQLDEDFGSSDCQSGPEETAKVSIRLSRGKPKVNKERLDTESAGQNDKTFFLPTMNEETFINHNCVMDPQGYPILPNGNTVYVRPPGANISNFGNVGFSKRTGTEYRSKGAWKLKRIYCLGVLSCDLPGCRWAGAPPTGRVVMSEWLEKNSHCRGARGTCLGTVVHIPCKQTLVRVDEHVATTWAVLRHRGIHDHPWPEAKKPDQLARDALKSVIASNPKAGAFALRIRKPNASKDSYESVLDIHPSLGNADRLRYYRKLMLKELNITPDNISGEVDDKFITDLFKWVKRGMLIISSSYLEGEEHFTFQSDWMQKRLLARNIDNLVYSTGLVSDVTYRFFRNGYLLSTSMYCEELARWVPIQLTWIRGLAEKYYERHFATLFRQFLRPDFTKAERDILVCNIVDFSAAQREGFVAAYWEVFGVCEKKVVLDKLQGCHEHYRAQVSRVKKNRHVVMADEESTFQTMCMDLIKKPVEGDPTHEEQIDALRRRFPKAKRWLDWWTMADVEAMLFPSRRPLLEDTPDGQDRPTKTTNAQESLHRLEGKTSLMLGMVQLYSFIKVLEEDFDAVMRGVSIEYGSARKGIDDISQSLGWEKKRKRAPNPAKPAVDEKVRHASVNDGRPPDTTDGLLPELAKKKLGRPSGSVNIDRNPFSTYPSYRASTQRHLANRCWMAAALETLYALYSPLWHRGSSGKGTSLFTSLVKHFSARTTYELTQAGSIRGALTKAQTSLFTQAQNRSPGSFVEGEFASADLFIEMLLDPKANPNRALKGLFDLEEKRVLTCQSHPGETKNLTRVVHTINIRREMFDDNQIPHANVTQLLRKWNSTSLQGLSGLTCKSCLAEQVIRHQSFVPVASDVEMSTSQVDSSVKGLYKESSTLVVPFGKAPPHLYFFLDVAPIIDNDEQQLFMATTDWPMSLFVSNQKYSLFARGYWNGSHYWSKVLKSVGGMVGVWLHNDLQNDGYARLVNVDPSKLSGPAPHTSWLMYSRCWTGPEKEYVDEMMDKIHKDYPHATGHLAFEGLSNLLESIKTDKPNLEESIITNKPNLVESIITDQPISQLDKHPKVPDKPVASPLPATIDYKPTSSQGGTPVAAGSDMVINPETTTVSQKKLKLKIKWSTPSAPKEDKEAATSGAGELTGKLNGIGSRRSKRVTKNGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3L695</accession>
    <name>E3L695_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transcription factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_18160</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178357">
        <property type="protein sequence ID" value="EFP92070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336489.1">
        <property type="nucleotide sequence ID" value="XM_003336441.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L695"/>
    <dbReference type="GeneID" id="10532027"/>
    <dbReference type="KEGG" id="pgr:PGTG_18160"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18160"/>
    <dbReference type="HOGENOM" id="CLU_009810_0_0_1"/>
    <dbReference type="InParanoid" id="E3L695"/>
    <dbReference type="OrthoDB" id="4456959at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172489" description="Transcription factor domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="411"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="455"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="641"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="739"/>
            <end position="789"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="142"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="425"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="455"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="498"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="657"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="739"/>
            <end position="748"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP92070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="806" mass="89261" checksum="D35DB7E20DAD37A9" modified="2011-01-11" version="1">MSNPRHTFALVVEFLRTAVTSGAHLEESARWNTSFLNNQLRKRAFFALITVEKNTTSSLGYVNVLQSFCNLVSPPIFVEDATMSLLDKQDGMQPTPEARKQFREQLYSRPQTPGEAAFNSLWSVRNKLESKSRNMILNQLKAQSSPSAQTTSSSTTTQDDPNSKTKNNRHLFILNFGTALWEFIENEMNPMGRWNPSLTSKRDLFATSYCACLLWSILIRVNLEAVSVYPELVHRCLQAASSILDTLEDMKVLGHLDLLRGDIPYFLAPVGLFFLWAISQDNNPLVTADFKANAWVRITKCVEILNIPAPVSFVSEKLSLKFTAYMNHLKEEIHNPSLDPGTSTNISKKRPISEIMAKKVEQPTISNLLFSQHASPANSDSYVDPVQAPPSYADFNFSDFMSTTGTSQFPSWNYDANPPVAATGNPNPNNQQNPYDPSSYSHLQLQNLLLQSGNVPQQPLNYNAPNIPLSTNRPQQFNPEGSTNSSLHEGSDPSHLRSTFPSSTSSIPPEVTKDQQIYSGINNDGLTPTSSGTNYIKTAGNFVNNQSFDRNLFTVGNASNKNSDSSFPFNSNFIPMNFSNPADEIFNPPQRLASINHPDPNFLNPTHPIVPNQAYNLPSSRPPEANAYPNFIPQNPVFNHSSSTNFTNHKPAMTPKSYPSHSHSQSLNFHPSAGPSTQKVSHINNQTFSKAQPFPPHPASSTSASFSDITFDVAPPFEFETQIDPNQKMFVSPYQINNDTGTSSGNDNNYRRRFAEGSSSLGPLTNLPPAGVPPQPVPFNGSANLSDGLQTDDQQMFSAICDTWLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>E3L6D5</accession>
    <name>E3L6D5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Glucanase</fullName>
            <ecNumber evidence="9">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_17415</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of (1-&gt;4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains.</text>
            <dbReference type="EC" id="3.2.1.91"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 7 (cellulase C) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="9"/>
    <dbReference type="EMBL" id="DS178358">
        <property type="protein sequence ID" value="EFP92110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336529.1">
        <property type="nucleotide sequence ID" value="XM_003336481.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L6D5"/>
    <dbReference type="STRING" id="418459.E3L6D5"/>
    <dbReference type="GeneID" id="10532070"/>
    <dbReference type="KEGG" id="pgr:PGTG_17415"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17415"/>
    <dbReference type="HOGENOM" id="CLU_020817_0_1_1"/>
    <dbReference type="InParanoid" id="E3L6D5"/>
    <dbReference type="OMA" id="FSIWNDN"/>
    <dbReference type="OrthoDB" id="412382at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016162">
        <property type="term" value="F:cellulose 1,4-beta-cellobiosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.100.10">
        <property type="entry name" value="Glycoside hydrolase, family 7, domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001722">
        <property type="entry name" value="Glyco_hydro_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037019">
        <property type="entry name" value="Glyco_hydro_7_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33753">
        <property type="entry name" value="1,4-BETA-D-GLUCAN CELLOBIOHYDROLASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33753:SF2">
        <property type="entry name" value="GLYCOSIDE HYDROLASE FAMILY 7 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00840">
        <property type="entry name" value="Glyco_hydro_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00734">
        <property type="entry name" value="GLHYDRLASE7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5 9" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="7 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173153" description="Glucanase" evidence="10">
        <location>
            <begin position="22"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP92110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="471" mass="52222" checksum="E3B8D946E06158B3" modified="2011-01-11" version="1">MKTSLCYLLLYFFQFQQNLVSQQVGIHLLQESPPSLKIQNCESDGKCSEIRMSVTLDADIRPLQVLQGMESCITDEEKAWDRRYCADSERCAEQCSLGGVKYKAVHGITTDDHTLKLRLFTHGKLTESRVYLLADQKHYQLFHLKNQQFSFEVDASKVPCGVNAALYFTSMKADGGLSRTNKAGAMYGTGYCDSQCPTALRFINGQANIEASGEPGEQVFKSMGACCPELDLWEGNGMVQAYGAHPCVFPSMTTCEGEDCTSKGGLCDHSGCGLASQRVDQNTFYGPNKTIDTRHKFTVVTQFLTSNATTQGELVEIKQFYIQHGVLIKHRGIKLPGQDSSLNSITDAFCSIMSNTTGTGSAFRRTGGLEAMGRAMEKGLVLVMGLWTDEVTDSLGSIQKIPATRKNSLKRINQLVCPEETRTQPFIQKHHPHASVLFSNIKVGPITNFPYNDLQDYSYQHQVHANEHTII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>E3L6Z1</accession>
    <name>E3L6Z1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_17990</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178362">
        <property type="protein sequence ID" value="EFP92316.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336735.2">
        <property type="nucleotide sequence ID" value="XM_003336687.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L6Z1"/>
    <dbReference type="GeneID" id="10537977"/>
    <dbReference type="KEGG" id="pgr:PGTG_17990"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17990"/>
    <dbReference type="HOGENOM" id="CLU_1653000_0_0_1"/>
    <dbReference type="InParanoid" id="E3L6Z1"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP92316.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="160" mass="17074" checksum="D8016C90E66F4726" modified="2012-04-18" version="2">MALGELSTFQSSDGMDAQNSDIWPLNVLASKRVSVRLSSSMTMFAGSEVLCPPIEFCSGHDPDSSYLSLEGFEEVEAPSSSAFGIRKPVHGLFRASVANRDPCKTTCQSTTGNGLLFKICSFLEAGGQGWQNARTKLSAWAARRPGGGLPCLADLLARCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3L7G6</accession>
    <name>E3L7G6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18316</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178365">
        <property type="protein sequence ID" value="EFP92491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336910.1">
        <property type="nucleotide sequence ID" value="XM_003336862.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L7G6"/>
    <dbReference type="GeneID" id="10538485"/>
    <dbReference type="KEGG" id="pgr:PGTG_18316"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18316"/>
    <dbReference type="HOGENOM" id="CLU_121077_0_0_1"/>
    <dbReference type="InParanoid" id="E3L7G6"/>
    <dbReference type="OrthoDB" id="10277464at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="194" mass="21262" checksum="8DEC6BCED173094A" modified="2011-01-11" version="1">MPSAPYSKSQLFLHESVSEPKPPLVNHTDKTAITTRFSQISSIMLLAGTILIMFSIISLPGAFAAATLPPVSSSTKISRNVGELVDKRIESNIQEKWKAIRAGVITKDPGSPRATKVKVFKDLPQEGNKISRVAQVEALASFKLARGLSLTRDVKSSELVISRAKKEAFKAIEQVAKERLKARPGLKLSWKSWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3L7T0</accession>
    <name>E3L7T0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18604</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178368">
        <property type="protein sequence ID" value="EFP92605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337024.1">
        <property type="nucleotide sequence ID" value="XM_003336976.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L7T0"/>
    <dbReference type="GeneID" id="10538764"/>
    <dbReference type="KEGG" id="pgr:PGTG_18604"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18604"/>
    <dbReference type="HOGENOM" id="CLU_1001622_0_0_1"/>
    <dbReference type="InParanoid" id="E3L7T0"/>
    <dbReference type="OrthoDB" id="10325224at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172514" evidence="1">
        <location>
            <begin position="23"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="278" mass="30948" checksum="AFD00DC9D88B1915" modified="2011-01-11" version="1">MDSRGDFILLGCCLALILTVSAITEFPRTSGNSEWWLHLTNNEINLNKNNIAPPSSHPDIQEVNPADIQQIHELWTELQQGPGISVIESQMGYPLVHQGIQTFDGGSQMGQITSYNPIDNPEAWESFQNIITSSTNHNQFASNSHGQHVESDNFDSSCPRNVEQLFSQGKQVAQFLPRYSTSETVHPEALQNSEKLVHPESFQNSENFLFPPSQTGVGYSEQFPSYSTLPMSDWKELGNKSVEMRDDRAFPINNISLNDIPQAFANPISNAPSSPEKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3L7Y3</accession>
    <name>E3L7Y3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18836</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178369">
        <property type="protein sequence ID" value="EFP92658.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337077.2">
        <property type="nucleotide sequence ID" value="XM_003337029.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L7Y3"/>
    <dbReference type="GeneID" id="10538986"/>
    <dbReference type="KEGG" id="pgr:PGTG_18836"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18836"/>
    <dbReference type="HOGENOM" id="CLU_1180703_0_0_1"/>
    <dbReference type="InParanoid" id="E3L7Y3"/>
    <dbReference type="OrthoDB" id="10376844at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92658.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="235" mass="26109" checksum="2DBFC8933C813C64" modified="2012-04-18" version="2">MTVQVTNLLPRSRLGPGNLAVARWGPGMHRSDGGFLLKKLRGFRWSNSRLLNSKNEGVGPQCLEDCRCTRPDTGRRGTKKSSRPQALWELLYADPMVIDDDSSSNSRPESIPFGPTTEWGNTQLAAPPVNAANPNPSARDMMEFDYNTIQNRLVGANPYPFCTQVYRKEDLLFLFQNVANNFIHLSNKYPASTLGEIDIAEILEEVESNLLRIAAIFAAAEVAARSADTRKLNIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8B2</accession>
    <name>E3L8B2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18566</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178373">
        <property type="protein sequence ID" value="EFP92787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337206.1">
        <property type="nucleotide sequence ID" value="XM_003337158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L8B2"/>
    <dbReference type="GeneID" id="10542500"/>
    <dbReference type="KEGG" id="pgr:PGTG_18566"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18566"/>
    <dbReference type="HOGENOM" id="CLU_038852_0_0_1"/>
    <dbReference type="InParanoid" id="E3L8B2"/>
    <dbReference type="OrthoDB" id="2507145at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="340"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="358"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="397" mass="44494" checksum="1D1F3C34AEA813A6" modified="2011-01-11" version="1">MTNFQVAEGSKDKEPTSTSSSADNALNGATLKENEVVEVQLTKDTGAAYRDLLSRHESESEGPLLIVNKAPDLNTSRSRSHPPTDKEKLWEKIVESKLANDDANTYFLLRIYLSLPREDATKNLRVPVELSDLRSTSAEAAIPTQKSTSSERTVKFIKGSVPNHCDIGFTPFFDKNIREFKGPLPLTIFDKEWQEDAVNFHSGKKSKSDEKDGIYTGYEYPNEWSQSFSAWTNNFRSFLITYRDIYKIPEFAEWIVAHKANVDEIIANEGFLTGFRYDMNIRANAFAYRVETSDGASVVDISVMRKDVRENTWAITRKLDELDFKDNPYAQGGVKCGFDPKTGRPKIGKGHKSFADANPYPSGNSSGYGSSSRGRAGFRKKSFGRGGYGYENGPDDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8Y9</accession>
    <name>E3L8Y9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">LAG1-DNAbind-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_18855</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Su(H) family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178379">
        <property type="protein sequence ID" value="EFP93014.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337433.2">
        <property type="nucleotide sequence ID" value="XM_003337385.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L8Y9"/>
    <dbReference type="STRING" id="418459.E3L8Y9"/>
    <dbReference type="GeneID" id="10542990"/>
    <dbReference type="KEGG" id="pgr:PGTG_18855"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18855"/>
    <dbReference type="HOGENOM" id="CLU_270956_0_0_1"/>
    <dbReference type="InParanoid" id="E3L8Y9"/>
    <dbReference type="OrthoDB" id="5600360at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1450:FF:000003">
        <property type="entry name" value="Related to J kappa-recombination signal binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.80.10.50:FF:000074">
        <property type="entry name" value="Related to J kappa-recombination signal binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1450">
        <property type="entry name" value="LAG1, DNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015350">
        <property type="entry name" value="Beta-trefoil_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036358">
        <property type="entry name" value="BTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040159">
        <property type="entry name" value="CLS_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008967">
        <property type="entry name" value="p53-like_TF_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015351">
        <property type="entry name" value="RBP-J/Cbf11/Cbf12_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037095">
        <property type="entry name" value="RBP-J/Cbf11_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038007">
        <property type="entry name" value="RBP-Jkappa_IPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10665">
        <property type="entry name" value="RECOMBINING BINDING PROTEIN SUPPRESSOR OF HAIRLESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09271">
        <property type="entry name" value="LAG1-DNAbind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20144">
        <property type="entry name" value="TIG_SUH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01268">
        <property type="entry name" value="BTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01267">
        <property type="entry name" value="LAG1_DNAbind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110217">
        <property type="entry name" value="DNA-binding protein LAG-1 (CSL)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49417">
        <property type="entry name" value="p53-like transcription factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RBP-J/Cbf11/Cbf12 DNA binding" evidence="8">
        <location>
            <begin position="449"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-trefoil DNA-binding" evidence="9">
        <location>
            <begin position="610"/>
            <end position="933"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="8"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="72"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="158"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="835"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="880"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="23"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="158"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="197"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="218"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="252"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="295"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="330"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="880"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="892"/>
            <end position="901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="937"/>
            <end position="947"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP93014.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1112" mass="119232" checksum="7AD7AC6177E8A44B" modified="2012-04-18" version="2">MVDYLYVSNGDKPFEPTTDDVHWTQTNNGFHSSTSPGPSSWAASQTMRESSAMFPSSTFYDQDADLTQVLHQQDESENHQSNSRKAHLSHSRSFPGLHQSPNHADLSSFRAASASLELAQPGEFNAYSFESHMNSSSSLFPPSGAITQQFYSQDPYNQYQEHQQASSNGLQPTLAGMNNFRSRSDSEGSGSWAGINTPMTPSDMNVNNGGYKPDLGSHGSSGFPNNNSHLRLNTSDLSAPQHPSSAPADSAFHNSRYSTSAQSTLPPSDSADLQPHSAQHAYALSGFPAPPVLQNVRSGSTGRRPQVGEDSSNDATITLNHDLHHPHRSLSPNTHNQALQPMSAGPAYSRSLSLESNAMSRNRSTSSNSAEFSENLHEWNPAVHPSSLPMSAPIGHQFSNLHLDPSDQLFAAFMSPKNMISRAEREALLAPFIRLYLSSTNRFMLGERTVLVLSGKVAQKSYGAEKRFLCPPPSALLLGCSWWAAADADPRRPMPSSNRLALHPPTTIVSMSGEHSIPTEAYSEWMSMSGHVVGDQASLDDVVIAGRCVGKQLHISEVDEKTKKVEALVRVIAPGFGPPEARHIGTFPSKPIKVISKPSKKRQSIKNLDLCIHHGTTIALFNRLRSQTVSTKYLCVSGPSASFPAGDWRAMSGMEEKPFAPGEDATCFVARTSAWDPFIIYLVDPSKPATVPDNVAPAYPPPPGYPRAPSNALPVSPNGGPIPIYYNQPVVLQCLSTAVVSPVMIIRKVDKGSTAIGGASLEGNASSGQRDMPVAPGEVLGDPVSQLHKIALEVMTDPQSAYLAPVTSPYAGFPGSGSFLACLGENVGVHRAESGRQAVPPIMTPPPSASSARSFGGTNSDALAEANYLASHLNQPILSSAGNSRARSNTPSSALDLDLGSSDGGKVKRPRKASASLLHGRALSKNRKRGASISSCGHDEHSSESECSHSNSLYPHASKVWTIECGEPAVWTIYVERHTFWIPPVLINGKPSSPVSPGAVSDVFTTPIPAAPIGYDSATPIPTISKYLPPNPAASDPQELKMIIIYGANLSPHLHVWFGDIPSPHVEHKCAEVILAQPPHHPMNGQSPTARQIILVSDDGIVFPSKVYFTSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3L904</accession>
    <name>E3L904_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Complex I-ESSS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">NADH-ubiquinone oxidoreductase ESSS subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PGTG_18870</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subunit">
        <text evidence="16">Complex I is composed of 45 different subunits. Interacts with BCAP31.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the complex I NDUFB11 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178379">
        <property type="protein sequence ID" value="EFP93029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337448.1">
        <property type="nucleotide sequence ID" value="XM_003337400.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L904"/>
    <dbReference type="STRING" id="418459.E3L904"/>
    <dbReference type="GeneID" id="10543005"/>
    <dbReference type="KEGG" id="pgr:PGTG_18870"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18870"/>
    <dbReference type="eggNOG" id="ENOG502S7S9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_138649_2_0_1"/>
    <dbReference type="InParanoid" id="E3L904"/>
    <dbReference type="OMA" id="WETIFYY"/>
    <dbReference type="OrthoDB" id="2147978at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019329">
        <property type="entry name" value="NADH_UbQ_OxRdtase_ESSS_su"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40637">
        <property type="entry name" value="ESSS SUBUNIT OF NADH:UBIQUINONE OXIDOREDUCTASE (COMPLEX I) PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40637:SF1">
        <property type="entry name" value="ESSS SUBUNIT OF NADH:UBIQUINONE OXIDOREDUCTASE (COMPLEX I) PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10183">
        <property type="entry name" value="ESSS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="7 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 17" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00030753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00031387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00046528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EFP93029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="127" mass="14681" checksum="AFD546A08ECE8DFA" modified="2011-01-11" version="1">MASHHIPRAFNKLTQQSTSLSRLASHPRLHRTSLLQQNVSGSLASSVRFGSGEPHYNQPSGWLFGEKPLKPGQKRVREDWELIWYIGFWGTTFVGIIMQIYKPDRSITTWARHEAEQKMEKPTYEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3LBY7</accession>
    <name>E3LBY7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20048</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178434">
        <property type="protein sequence ID" value="EFP94062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338481.1">
        <property type="nucleotide sequence ID" value="XM_003338433.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LBY7"/>
    <dbReference type="GeneID" id="10535759"/>
    <dbReference type="KEGG" id="pgr:PGTG_20048"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20048"/>
    <dbReference type="HOGENOM" id="CLU_070137_0_0_1"/>
    <dbReference type="InParanoid" id="E3LBY7"/>
    <dbReference type="OMA" id="DGHWIEL"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP94062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="246" mass="27188" checksum="08123887E17FE1DA" modified="2011-01-11" version="1">MTPNSTSSDSESAADQPGPSPGGVLPVEESVEPQASALEPQLHEEHDEAPATPQRHLPPHAEHFGEGNWIQLPEELNGTIRALLANLQGPTEIELDEEGAPRLPDAQDEPAVAQNALALIRHLEAMPAIVAGEPHQIDANPWQQMLQTLTPEQRRVVENFENRTITVRRELANNSVQSNDPMAVLQGYFFHLEELRRTLARLPQETANMIEQFNSNAFQEIGRLMVNSDHIDVAALHIGYLLDSLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3NXI1</accession>
    <name>E3NXI1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20228</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS989941">
        <property type="protein sequence ID" value="EFP94280.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338699.2">
        <property type="nucleotide sequence ID" value="XM_003338651.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NXI1"/>
    <dbReference type="GeneID" id="10527409"/>
    <dbReference type="KEGG" id="pgr:PGTG_20228"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20228"/>
    <dbReference type="HOGENOM" id="CLU_040957_1_0_1"/>
    <dbReference type="InParanoid" id="E3NXI1"/>
    <dbReference type="OrthoDB" id="10309749at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="241"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP94280.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="320" mass="34496" checksum="650B887BF2DB9A74" modified="2012-04-18" version="2">MSDKDKVANRPLSFRSNFRPLEESVPTIKPGQIYGSVSTPSFVTCNANDAVDYRVSLITTGTAAHVLSPDSVYGLSGRVLLLNGPAPPIFNYYIELVTKICSAANQIEDSTDRTFTSGVGLVVGLLNSNVDADLEVQSGKKQDLHVQVNHRDWDPTNFNVKYIIPANPKLVNTHIMIRVGREFVFDGYLSGWDLKDHSAIITVLGFSPINMGFSSPGPKTNPSSPRTSPATKGRKFITFEESDSKVSQPSVQSANSNVDGNDFRTPVASTSGYSSRPGLLEAELASDPAQAVGLIEKGKTKAVDSPPVVKKRRRAAGIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3NXX0</accession>
    <name>E3NXX0_PUCGT</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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        <lineage>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS989987">
        <property type="protein sequence ID" value="EFP94419.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338838.2">
        <property type="nucleotide sequence ID" value="XM_003338790.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NXX0"/>
    <dbReference type="GeneID" id="10527631"/>
    <dbReference type="KEGG" id="pgr:PGTG_20375"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20375"/>
    <dbReference type="HOGENOM" id="CLU_2339289_0_0_1"/>
    <dbReference type="InParanoid" id="E3NXX0"/>
    <dbReference type="OrthoDB" id="10427895at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP94419.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="98" mass="10713" checksum="6C4ECD9E42603D3A" modified="2012-04-18" version="2" fragment="single">FPVKAHIIAICGKADIYPGGNVYQIDGDREDRMMELESPENFARCVCRTRNRGRPPPRCVAEQRLGGVFTLHGSHGVPGYAEVAPGSPGLLWISHVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>H6QNR9</accession>
    <name>H6QNR9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_20623</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EHS62501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890818.1">
        <property type="nucleotide sequence ID" value="XM_003890769.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QNR9"/>
    <dbReference type="STRING" id="418459.H6QNR9"/>
    <dbReference type="GeneID" id="13542536"/>
    <dbReference type="KEGG" id="pgr:PGTG_20623"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20623"/>
    <dbReference type="eggNOG" id="ENOG502S1WT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066701_1_0_1"/>
    <dbReference type="InParanoid" id="H6QNR9"/>
    <dbReference type="OrthoDB" id="5149081at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052055">
        <property type="entry name" value="Hepadnavirus_pol/RT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050:SF7">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS62501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="292" mass="33937" checksum="4CFACBD304B0FE0A" modified="2012-04-18" version="1">MKTIAERSVKLGVATSVEKCSEFAEEQKFIGFIWNGVSKTVRLTEIKLDKRKEQIKEFLHPKREFKFNEVKILAGRLNHVSLLLPQLRCYSRSIYRWMNEWKKKWATRAPPEDVIEDLSFWLSTLNDYSSTRLCPLTDPTKINWVGDASTGFGIGVLIGRRWSQLRLKENWAEASPRRTIAWLETVAIRIGILMLLSIGKNLKGRNFIVWTDNTTTESTLRSRKSKDFHSNNEWKQIQKLLIFEDLDITPLRVTSAENVSDGLSRGVQRPHVAENRVWFNIPDNLSNFMFHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPX5</accession>
    <name>H6QPX5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Magnesium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_20943</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Mitochondrion inner membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EHS64477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890391.1">
        <property type="nucleotide sequence ID" value="XM_003890342.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPX5"/>
    <dbReference type="FunCoup" id="H6QPX5">
        <property type="interactions" value="222"/>
    </dbReference>
    <dbReference type="STRING" id="418459.H6QPX5"/>
    <dbReference type="GeneID" id="13542650"/>
    <dbReference type="KEGG" id="pgr:PGTG_20943"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20943"/>
    <dbReference type="eggNOG" id="KOG2662">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_516929_0_0_1"/>
    <dbReference type="InParanoid" id="H6QPX5"/>
    <dbReference type="OrthoDB" id="10251508at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045016">
        <property type="term" value="P:mitochondrial magnesium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12823">
        <property type="entry name" value="Mrs2_Mfm1p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.340:FF:000031">
        <property type="entry name" value="Chromosome 12, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039204">
        <property type="entry name" value="MRS2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890:SF0">
        <property type="entry name" value="MAGNESIUM TRANSPORTER MRS2 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13890">
        <property type="entry name" value="RNA SPLICING PROTEIN MRS2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22099">
        <property type="entry name" value="MRS2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="179"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="239"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="508"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="179"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="208"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="242"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU366042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS64477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="527" mass="58510" checksum="1FD3B01342565E2A" modified="2012-04-18" version="1">MVNSRLGSIGTLRPFSTKKRLIPSFLPSNRTLEFRLNNNNNNNNHLKQNKHKQPSTILRFNQQNSTIKPPPDPIESIRDNIDKPFLASRNSLPRRDDLSAIRSILIDRNARILSSRALSKSEICAQNRLQPRDLRKIDSRISNVVPSILVRDEAIIFNVLNIRALIRADSILIFEDPSSPSLSHNHTSSPSTTSSSSTATSSEKSTSVNEKASPESSATTRYSIRSAFLHNLLNNLVDHHNPNQAENSHEQPNQCSPKSTELPYEFRALETMLGSVATTLESELGVLKTLVSSLLDGLEQNIEREKLKQLLLYSRRLSAFNSRALLVQRCLDEILENEQDMANAYLSEKILNKSPRQVHDHEEFEQLLESFSKYVEEIVHEGTSTLTNIKSTEEIIDLILDSNRNTLLALDLKVSIGTMGLAVGALTAGLFGMNLRTHMEADPYAFYVVTGLTLVGVMSTIGYGCRRLYRLRRVGLSSVTSPSSSAIAAAGCSRRIISTGSAAKNGGILDWDVPRHPSHSHSNSSSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQ55</accession>
    <name>H6QQ55_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21007</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EHS64656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890362.1">
        <property type="nucleotide sequence ID" value="XM_003890313.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQ55"/>
    <dbReference type="GeneID" id="13542079"/>
    <dbReference type="KEGG" id="pgr:PGTG_21007"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21007"/>
    <dbReference type="HOGENOM" id="CLU_1190383_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQ55"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="233" mass="26482" checksum="1E43024297FF779E" modified="2012-04-18" version="1">MQIRIGRQPRGLQLRNAGQYSKLEGGLMLRQARTDWIGATGEASVCAGLCWTDDKHNYGQQEERTKFKAEKSDLILQHTEQIKSMDSRHEDQINNLKAKHKEILVASLTELDLRRTRKFESNLSALQEKHLAEINEIKLDHQKQIKAITANPETSGGPKSGTPDGDSMITYPSELAKIKSSYEEKIEMLKLQHQTDLNALEEKFLMSSLSADLRKEQQANQRKINLLTDELGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQW4</accession>
    <name>H6QQW4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21280</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EHS62930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890090.1">
        <property type="nucleotide sequence ID" value="XM_003890041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQW4"/>
    <dbReference type="GeneID" id="13540871"/>
    <dbReference type="KEGG" id="pgr:PGTG_21280"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21280"/>
    <dbReference type="HOGENOM" id="CLU_2414335_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQW4"/>
    <dbReference type="OrthoDB" id="10270155at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003606519" evidence="1">
        <location>
            <begin position="24"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="92" mass="9781" checksum="A56528BCB2F15443" modified="2012-04-18" version="1">MMHSLQLIGAFMVSFSLIGSLVASSPEVKVECGNARVLNLKAPNRVCKSGTTFYDMIASVPCKPKEHSLCCVRRVDSGSFIPLGCTSMGGVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRB8</accession>
    <name>H6QRB8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ferric-chelate reductase (NADPH)</fullName>
            <ecNumber evidence="3">1.16.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_21438</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2 a Fe(II)-siderophore + NADP(+) + H(+) = 2 a Fe(III)-siderophore + NADPH</text>
            <dbReference type="Rhea" id="RHEA:28795"/>
            <dbReference type="Rhea" id="RHEA-COMP:11342"/>
            <dbReference type="Rhea" id="RHEA-COMP:11344"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.16.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ferric reductase (FRE) family.</text>
    </comment>
    <dbReference type="EC" id="1.16.1.9" evidence="3"/>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EHS63109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889970.1">
        <property type="nucleotide sequence ID" value="XM_003889921.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRB8"/>
    <dbReference type="FunCoup" id="H6QRB8">
        <property type="interactions" value="167"/>
    </dbReference>
    <dbReference type="GeneID" id="13540544"/>
    <dbReference type="KEGG" id="pgr:PGTG_21438"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21438"/>
    <dbReference type="eggNOG" id="KOG0039">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017408_0_0_1"/>
    <dbReference type="InParanoid" id="H6QRB8"/>
    <dbReference type="OrthoDB" id="17725at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000293">
        <property type="term" value="F:ferric-chelate reductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000116">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051410">
        <property type="entry name" value="Ferric/Cupric_Reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361:SF9">
        <property type="entry name" value="FERRIC REDUCTASE TRANSMEMBRANE COMPONENT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32361">
        <property type="entry name" value="FERRIC/CUPRIC REDUCTASE TRANSMEMBRANE COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0249">Electron transport</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="5"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="10">
        <location>
            <begin position="25"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS63109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="351" mass="37853" checksum="B7147D2A742DF8FC" modified="2012-04-18" version="1">MYQTFLLCHIFGFSAFLLALWFHVPALKPIVVLSASFVGFDYLLVYLKTSIRSAVFTSLPGGVTKVEIDRIGEGWTAGQHVYLRVFKGRHSFEKHPFTIANAPASLSPSGRNTLVLMAKAAGDFTSRIHRVGYAEAALVGGDLESRKASVDEKHFQALCEAASDCRLAVAVEGPYGASYLDMSDHETVVLVAGGSGFTYCMSTLEHIIGNAMKGVGLTKKIFVLWTLRDLDMVQVFASDLNRALECARQFRFEVVVRLYITTPLKHGDMNPVPNAQITPNRVDLKGVLSEALESTCWSIDTRGETKGCGLAVGVCGPEGLVVSARSALSAADPVLAAKAGGLQMHSEVFGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRS2</accession>
    <name>H6QRS2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_21544</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EHS63369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889807.1">
        <property type="nucleotide sequence ID" value="XM_003889758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRS2"/>
    <dbReference type="STRING" id="418459.H6QRS2"/>
    <dbReference type="GeneID" id="13543055"/>
    <dbReference type="KEGG" id="pgr:PGTG_21544"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21544"/>
    <dbReference type="eggNOG" id="ENOG502SBJ3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_559139_0_0_1"/>
    <dbReference type="InParanoid" id="H6QRS2"/>
    <dbReference type="OrthoDB" id="2100128at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39398:SF1">
        <property type="entry name" value="CSN8_PSMD8_EIF3K DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39398">
        <property type="entry name" value="YALI0F14311P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="89"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="257"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="9"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="24"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS63369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="488" mass="57414" checksum="FFAB0BDE81F81D3F" modified="2012-04-18" version="1">MSLTNSRWNQPEQEQEPNNNLKTNSKHSKYIPPHSKPKTTNKNIQNNSNNSIIITNGDRMVSKKFGNQHQQQQDTKLTGFMNSFNLSNPRLDPMSLVPSPSRGIYSSENQTKKSSTPEHDNQDLTNYDFQVEFFKWILNRIQEYKKAYRNRSDLFLYLSLKDSHEDPEKLKNQGEDDEGHYKTQIDRISSLVREIRKLREGIFASGRRDSFAIVVYELSAELALESLDFAQLNTLLNHLIMNLYRTLSISDHLIPDSNKQQQEKEDLETLINHHRRTKEEVLNRRLMFARVYLLLPIASRLDISRFIDQFAQITQILSDQTLHNLDHYAHSTAHKQPFSDISGPLHPKLLELKTFFKLVLRKNYFQLNQKFIKPKLKKIITTRHHQEEEEDESMTTEWDELLFLVFLSSIRLNLIWKVIAKSYYHLSLEDDRFIVDLLSLDFQSFNHFRNSSHHPSASSNNLNHHQLVNSWLLKLNIQRNPNGIIQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSH3</accession>
    <name>H6QSH3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21788</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178294">
        <property type="protein sequence ID" value="EHS63710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889532.1">
        <property type="nucleotide sequence ID" value="XM_003889483.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSH3"/>
    <dbReference type="GeneID" id="13542758"/>
    <dbReference type="KEGG" id="pgr:PGTG_21788"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21788"/>
    <dbReference type="eggNOG" id="ENOG502S7P8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005992_2_0_1"/>
    <dbReference type="InParanoid" id="H6QSH3"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="452"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="504"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="620"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="461"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="509"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="529"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="692" mass="76669" checksum="027C420298BD4B02" modified="2012-04-18" version="1">MHRVYYMLSTGKKSLMIGMIELYAFVTALEKDWVQVRRGIPISYGSLPPKQVNVSHSIGWNKATKRQKAAWNDGRAPDTTEQLLANAENLPKRAKLGRPKNSPNINRDPQSTPLWLQEPGGKQTDLFFLLVSHFTTRTTYEMKEGAQVRSILTRGSNKLFMEAQKLYPANFISGAFASCDLFLEIVLDPKRNKSKTLPKLFRVQEDRIFSCPLHPGAQEHPRGSRDLGVLSVTKSMFESNNINCANVAELFSQWTTTGLVGVSGLQCRQCNQENSQIQSPAITSNLLHEKSIISVINSTPPAHLYFQVDVALISSEDKQIDFMGNINWPFKVNFAGEEYTAVSRGFWGDSHYWGKVLRYVNGVTAVWMHDDRANGGFAQLVDRTPGSISGAQPHTSWMIYSRQWTPNEKAFIDTSIANIQRDNPVVKADNIPFVNMGAVLQATFDSALPHLTQGQAADPNPTMTITQLNGESGPQDGNNREKPVNSTGEGLKIRIRRIDKKAASNPLNSVSTSAPSVSSKELMSAAPPGSTNLPTESLPSLRLGSKLPFLDVKSKKTTGKCGRPKARKLASKTFIPKPLTDADWARISNTYWNNRWLEKEELASNSKPVAAIGSSTIQGDSQAPEIPQLHSPHGARTGSQALRRSSRKRGFKVVRLCMGVFASVDMCCVGEQFYKGFRKADLLQRMINCTNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTG9</accession>
    <name>H6QTG9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22093</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178313">
        <property type="protein sequence ID" value="EHS64184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889200.1">
        <property type="nucleotide sequence ID" value="XM_003889151.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTG9"/>
    <dbReference type="GeneID" id="13541182"/>
    <dbReference type="KEGG" id="pgr:PGTG_22093"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22093"/>
    <dbReference type="HOGENOM" id="CLU_2360745_0_0_1"/>
    <dbReference type="InParanoid" id="H6QTG9"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="54"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="96" mass="11520" checksum="AF22CB0BCFFAF9C4" modified="2012-04-18" version="1">MEGTCEIIPLSGNCQRCQEYRVRCSFQSRTTLIETLYQNIMELRTRLALREWAVKEREEEYNKLSEEYEKIKKNYAELFDIAYKNSPSEQAEQPEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTV8</accession>
    <name>H6QTV8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22229</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178323">
        <property type="protein sequence ID" value="EHS64372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889044.1">
        <property type="nucleotide sequence ID" value="XM_003888995.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTV8"/>
    <dbReference type="GeneID" id="13541805"/>
    <dbReference type="KEGG" id="pgr:PGTG_22229"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22229"/>
    <dbReference type="HOGENOM" id="CLU_694711_0_0_1"/>
    <dbReference type="InParanoid" id="H6QTV8"/>
    <dbReference type="OrthoDB" id="2506601at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="209"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="397" mass="42777" checksum="F4D5244D24FA1237" modified="2012-04-18" version="1">MSARSSTWSESAASELDPLFLSLKEPAGTPRRTRSRPSLNPLPSPSTSSDSPLPIPATVNFPLPPRRLRPGELIGIPDPAPTSSISPADRPDPFLAPVSLYGVAIGGNNPIKTNPAVNGRAEVKTAGLTSLDLRKMSLRNRSSASISHPPCPPPETISLRARNPSRMLAPHTPSLHSPPRSYSHNTQQQHPPRYQQAFSASTSNLITTPSVERSVRSSSRPSPQSSLPPRNLSLVETSSNPIPRLPPTRLAYQSLSYNLSTTPEPFDHSLSSIRQPLSESLSSDLNNFNPNPQLRRHPLLSASPIPSQNASDSLAIYSGMAPITDRSNGRIKFAPLPNKPGFAGPSVIPSLSLFESQNTQPKQQNSSRKAAWIQRWLMPATKPVVSQTQTGHLLAGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>H6QVL7</accession>
    <name>H6QVL7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22789</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS989954">
        <property type="protein sequence ID" value="EHS63311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890883.1">
        <property type="nucleotide sequence ID" value="XM_003890834.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QVL7"/>
    <dbReference type="GeneID" id="13541063"/>
    <dbReference type="KEGG" id="pgr:PGTG_22789"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22789"/>
    <dbReference type="eggNOG" id="ENOG502TEVT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2543688_0_0_1"/>
    <dbReference type="InParanoid" id="H6QVL7"/>
    <dbReference type="OrthoDB" id="2153847at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="83" mass="9638" checksum="65E25CA4A5FFA44B" modified="2012-04-18" version="1">MVKEIRVEKISKNSLKVLEELLFQQLPSKVMISVYKEKFKKLNAALIRACDRQSNRCADFFNKKGQGKTADCNAQLDRFQNKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>E3JPU7</accession>
    <name>E3JPU7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Iron-sulfur assembly protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_00516</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in the assembly of mitochondrial and cytoplasmic iron-sulfur proteins. Probably involved in the binding of an intermediate of Fe/S cluster assembly.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HesB/IscA family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74560.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307566.2">
        <property type="nucleotide sequence ID" value="XM_003307518.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JPU7"/>
    <dbReference type="STRING" id="418459.E3JPU7"/>
    <dbReference type="GeneID" id="10527521"/>
    <dbReference type="KEGG" id="pgr:PGTG_00516"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00516"/>
    <dbReference type="eggNOG" id="KOG1120">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069054_0_1_1"/>
    <dbReference type="InParanoid" id="E3JPU7"/>
    <dbReference type="OrthoDB" id="333486at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.300.12:FF:000001">
        <property type="entry name" value="Iron-binding protein IscA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.300.12">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016092">
        <property type="entry name" value="ATAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000361">
        <property type="entry name" value="ATAP_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050322">
        <property type="entry name" value="Fe-S_cluster_asmbl/transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017870">
        <property type="entry name" value="FeS_cluster_insertion_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035903">
        <property type="entry name" value="HesB-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00049">
        <property type="entry name" value="iron-sulfur cluster assembly accessory protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10072:SF41">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY 1 HOMOLOG, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10072">
        <property type="entry name" value="IRON-SULFUR CLUSTER ASSEMBLY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01521">
        <property type="entry name" value="Fe-S_biosyn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89360">
        <property type="entry name" value="HesB-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01152">
        <property type="entry name" value="HESB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core" evidence="5">
        <location>
            <begin position="194"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="99"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="104"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="153"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00054873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00071673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP74560.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="302" mass="32995" checksum="7D735A72BE371010" modified="2012-04-18" version="2">MSSSIPYSLIQKLTQPSSSSSSLLSRTNRLFHHQSPSTSIHPGQTGILLRRSISHRFSHSQPSSSHQEHPSISLNQTRNLEISNSNQVIHQSISSVNQNNLKLPRSSSSTISQPSEPSHEDILINSLPTSYNSSSTTTTASSSSSLEPPSFEPLQTQRETTSSAPNLTQQSPTKAPTSQVKRTTRTSKPAKQVINLTPRAIGHLSTLLDGPNPKLIRIGVKNKGCAGMAYNLDYVDQPGKFDEVVVTKNEEGKEIKVLIDSRALFSIIGSTMDWQESKLGNKFVFDNPNIKEECGCGESFLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3JR79</accession>
    <name>E3JR79_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycoside hydrolase family 71 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00147</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307197.1">
        <property type="nucleotide sequence ID" value="XM_003307149.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JR79"/>
    <dbReference type="STRING" id="418459.E3JR79"/>
    <dbReference type="GeneID" id="10528125"/>
    <dbReference type="KEGG" id="pgr:PGTG_00147"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00147"/>
    <dbReference type="eggNOG" id="ENOG502RTMK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019141_4_0_1"/>
    <dbReference type="InParanoid" id="E3JR79"/>
    <dbReference type="OMA" id="NDYERSD"/>
    <dbReference type="OrthoDB" id="3257981at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051118">
        <property type="term" value="F:glucan endo-1,3-alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11577">
        <property type="entry name" value="GH71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005197">
        <property type="entry name" value="Glyco_hydro_71"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03659">
        <property type="entry name" value="Glyco_hydro_71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172699" description="Glycoside hydrolase family 71 protein" evidence="1">
        <location>
            <begin position="17"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP74191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="450" mass="49738" checksum="5A704D77FF031B35" modified="2011-01-11" version="1">MPLSWCLLILLNYSRSDWSSQCNLAASHGIDAFALNVGLPDGWQLQQVKNAYEVADAIKTSTGKSFKLFLSLDMSVIQSAEDVTTWVTSFVPLSAQLLIDGRPLVSTFSGEANKLGGEDLSSGWQAALKAPLAQGNPPLDALFIPAWSSLDPSTAVSENPVVDGIMTWNSWPTGSEEINTLDDYQFQTNARDSKKLYMAGVSPCFFTHYKDKNWIYKSDDNLYISRWMKLISMPNAPDFIQIISWNDYGESHYIGPRLGTPPADTTWLDGFDHQSWLKMSDYFIKWYKAGARPTIEEDSVHFNYRPHSVNAIASADTLPPPTNASVSVDAIYASTYLSAASTAAQLRIRVGDMQQTFTNITKSEVNTFTAPWNGHTGRVEVALLDNTGKELMKKHGEVPISNDIKTYNFNYAADVLSKSASGTAPALLVLPRVHIVLSLFISQVAIYQMC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3JR97</accession>
    <name>E3JR97_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Thioesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_00394</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the lcsJ thioesterase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74438.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307444.2">
        <property type="nucleotide sequence ID" value="XM_003307396.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JR97"/>
    <dbReference type="GeneID" id="10546472"/>
    <dbReference type="KEGG" id="pgr:PGTG_00394"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00394"/>
    <dbReference type="eggNOG" id="KOG4366">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043860_0_0_1"/>
    <dbReference type="InParanoid" id="E3JR97"/>
    <dbReference type="OrthoDB" id="265761at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00586">
        <property type="entry name" value="4HBT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.129.10">
        <property type="entry name" value="Hotdog Thioesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029069">
        <property type="entry name" value="HotDog_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051490">
        <property type="entry name" value="THEM6_lcsJ_thioesterase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12475">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12475:SF4">
        <property type="entry name" value="PROTEIN THEM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13279">
        <property type="entry name" value="4HBT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54637">
        <property type="entry name" value="Thioesterase/thiol ester dehydrase-isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="79"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="283"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP74438.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="348" mass="39443" checksum="6E68C918B5127020" modified="2012-04-18" version="2">MTAGPTETEARGTEAGKSGTTSDGSPSAGRRERKRGTKMSEELARFRPRPSTILVSLLALRYGRPGPSRGAPRRLLHWFALWLAVVNWRILPFYWHAMTLLFPILKAKLRRWKLGSSTPAFVANLHVLGKSPFQARIVSKHCATWNTCDFMFHMSNSAYAIALDEARAIWQIQMIGAAMRADHERVRPMIASTHFTYFAEIPILADFEVEFRPVAWDHKWLYLLATFTTDPPKGSRTRPLNCLSITRMVNKIGRRTVRPEKLMALSGLGMDPSEWETLSRLRTPDPATRHPAPSSNTSHLNPAQEWLVSSDPFEPFQKYEALRTRNLEIIRAGLDGDLKIGAERLKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3JSM6</accession>
    <name>E3JSM6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01644</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319470.1">
        <property type="nucleotide sequence ID" value="XM_003319422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JSM6"/>
    <dbReference type="GeneID" id="10547413"/>
    <dbReference type="KEGG" id="pgr:PGTG_01644"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01644"/>
    <dbReference type="HOGENOM" id="CLU_128888_0_0_1"/>
    <dbReference type="InParanoid" id="E3JSM6"/>
    <dbReference type="OrthoDB" id="10304395at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173162" description="Secreted protein" evidence="1">
        <location>
            <begin position="32"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="143" mass="15657" checksum="F6E0D2E8E6D3E6EC" modified="2011-01-11" version="1">MFEMRSSMLNARVALASVIIAATLCVQLSEARHSLVPRVCLTAPGQSYSCSRCNSGDDNSIFSQMHWVHCSANQNPGGVGGSDVTIVETSYFNVNHDTKLIGVQGREEGGSSQTYYTCPMSSNLQRPKTERQALNLYKVGYRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUG8</accession>
    <name>E3JUG8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01024</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320112.1">
        <property type="nucleotide sequence ID" value="XM_003320064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JUG8"/>
    <dbReference type="GeneID" id="10543573"/>
    <dbReference type="KEGG" id="pgr:PGTG_01024"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01024"/>
    <dbReference type="HOGENOM" id="CLU_154039_0_0_1"/>
    <dbReference type="InParanoid" id="E3JUG8"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="1">
        <location>
            <begin position="18"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="114" mass="12926" checksum="7E4F2A1B83989A13" modified="2011-01-11" version="1">MGEILVICCPVLGHFYRSKKLDRPTGNKKAKFLNLLAVKDLEWKEDVASAHKKLATESTRLKDIFTNDLQSLKLMADNGETAAELTIMTQSLNGLDDEQQEYFKLKRAQILQSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUM6</accession>
    <name>E3JUM6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_01082</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="1"/>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75751.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320170.2">
        <property type="nucleotide sequence ID" value="XM_003320122.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JUM6"/>
    <dbReference type="STRING" id="418459.E3JUM6"/>
    <dbReference type="GeneID" id="10543743"/>
    <dbReference type="KEGG" id="pgr:PGTG_01082"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01082"/>
    <dbReference type="HOGENOM" id="CLU_642715_0_0_1"/>
    <dbReference type="InParanoid" id="E3JUM6"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:004188">
        <property type="entry name" value="Adenosinetriphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000145">
        <property type="entry name" value="probable ATP-dependent RNA helicase DHX40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.2130">
        <property type="entry name" value="DEAH helicase family, winged-helix domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042035">
        <property type="entry name" value="DEAH_win-hel_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056924">
        <property type="entry name" value="SH3_Tf2-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF83">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR ATP-DEPENDENT RNA HELICASE DHX16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24626">
        <property type="entry name" value="SH3_Tf2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="104"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP75751.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="465" mass="51990" checksum="1CAF7ECD5873AF9E" modified="2012-04-18" version="2">MTDGVGMLLREFTTKPNLAGYSAMIIDEAHERTLSTDILLGLVKDIARFRPDFQLLICSVTMNAVKFSEYFYDAPIFNILGKMYPVDIMYTPNPEANYLYAAVAIIFQIHTTKPKADILVFFTGQDEIRASQENLEETARALGNKIGELMICPIYANLPTNMQAKIFEPTPDRAQKFLLATNISKTLITINGVVYVIDPGFVKQNSYNPCTGMESLVFFLFCLPAAANQRAGRAGRVAPGKCFRLYTKSAYMKEIDKDTVVEIQQTNLANVVLLLKSLGINNLIGFDFLDPPPGMTAYIGRAGRHANLKGIPPAGEWRACLHCWIDQSSVEEQIKRIDEVQSELKLCLEAAQESMKAQFNKGVKDTPNWNVGEEVWLNSKNISTTRPSPKLDHQWLGPLQISKKISNSVYKLTLPLSMQGVHPVFHVSVLQKHNSDTITGRCHPSPEPVQVNNEEEWEVEGVLDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUN7</accession>
    <name>E3JUN7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_01093</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75762.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320181.2">
        <property type="nucleotide sequence ID" value="XM_003320133.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JUN7"/>
    <dbReference type="STRING" id="418459.E3JUN7"/>
    <dbReference type="GeneID" id="10543754"/>
    <dbReference type="KEGG" id="pgr:PGTG_01093"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01093"/>
    <dbReference type="HOGENOM" id="CLU_040058_6_0_1"/>
    <dbReference type="InParanoid" id="E3JUN7"/>
    <dbReference type="OrthoDB" id="2506498at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000675">
        <property type="entry name" value="Cutinase/axe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33630:SF9">
        <property type="entry name" value="CUTINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33630">
        <property type="entry name" value="CUTINASE RV1984C-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01083">
        <property type="entry name" value="Cutinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01110">
        <property type="entry name" value="Cutinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="159"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="188"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP75762.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="222" mass="21897" checksum="D2426726B51DD10F" modified="2012-04-18" version="2">MAKSVLAAVPGGGLMDIQYSSSAEYMKSPKDGAQTGLKYLKAQASKCPKMAMVQTQILAAKDLPPNKVVAVALFGNPYFTAGAPQNKCDAKTGKGIASSMGVKAPKQYTSIIWDCCLTGDMICQTSGGMTSHMKYGGKPASDAQAFIVGKLKAVKAGGSPGGAAGGNTNGKGGADSGGGAAGKSMGSKPGGDMGGGKMGGLGGKLGGLGARSLAWHAASRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3JW22</accession>
    <name>E3JW22_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02688</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
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            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="KEGG" id="pgr:PGTG_02688"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02688"/>
    <dbReference type="HOGENOM" id="CLU_1046397_0_0_1"/>
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    <dbReference type="OrthoDB" id="2498987at2759"/>
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    <feature type="compositionally biased region" description="Basic residues" evidence="1">
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    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP76247.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
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            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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            <end position="100"/>
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    <evidence type="ECO:0000256" key="1">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76352.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="134" mass="14864" checksum="317495741646990D" modified="2012-04-18" version="2">MFMSGELTQPNITQPNDWDPPYLQLLPAFQVPTASSSASTSQKTEALSKDPLGSIHPLDGYPTYSLRPEHEALNLEVAAPKPRPDAHHQPRPSLTTDSLHPEHEALNLLVAAPKPRPDAHLFIFVNFLFDNHCL</sequence>
</entry>
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    <accession>E3JXS8</accession>
    <name>E3JXS8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GTPase of the mitochondrial inner membrane that associates with the large ribosomal subunit</fullName>
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
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    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006169">
        <property type="entry name" value="GTP1_OBG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036726">
        <property type="entry name" value="GTP1_OBG_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045086">
        <property type="entry name" value="OBG_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702">
        <property type="entry name" value="DEVELOPMENTALLY REGULATED GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11702:SF31">
        <property type="entry name" value="MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01018">
        <property type="entry name" value="GTP1_OBG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82051">
        <property type="entry name" value="Obg GTP-binding protein N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51883">
        <property type="entry name" value="OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Obg" evidence="5">
        <location>
            <begin position="78"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="OBG-type G" evidence="4">
        <location>
            <begin position="337"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="391"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="10"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP76853.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="610" mass="67266" checksum="D8EE2C732C28F3E4" modified="2012-04-18" version="2">MIIRKPAKLQTRTAHQHQPFSSLSVLHRPSTNSDQQTRTGSSTDPQQGNQTEWKSSFDWEAELRRRQRVAKERGRKASKFVDQMKVLVRAGNGGDGGVAFHREKFVARGGPSGGNGGLGGSVYLRPSAEVQSLNRIPRILKAASGTNGGGQWMNGPAGKDLIVDVPIGTVIREIRSSQISAQRESALGFNLGHRPPKASDLVGEDIEVAKARRSKLFVHYPESEDSNQTNEALKNLEIDLLQEQWATERARSQSEPFQMDCAKFHEDHRSRTNIEHPSTQVNRSIKAEQDFFLIAKGGEGGLGNSNFSGNQTTLPRFATRGKKGEVIELELELKTLADIGLVGFPNSGKSTLIHTLTNSRAEIAPYPFTTLNPQIGTLIIFNDGSWDLDEPTDAINHSPSHREYLDANSATDLIRDRSNRQRSSSHSPRKCESIRLTIADCPGLLPKASENVGLGHAFLRHIERSRMLVVVVDLMAGLPTATTATTSSSSSSLTNQRLIEDENPQRCCQDVETLFKELESYQPGLSGRVGILIANKADLSSTDPRLQEIAQVRLQALQDYVHALSSFQVQQSIRNLGTPHISVLPLSAKFRLNVRKLVLVIKEFFEASKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>E3JYZ8</accession>
    <name>E3JYZ8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribosome assembly factor mrt4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_03229</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Component of the ribosome assembly machinery. Nuclear paralog of the ribosomal protein P0, it binds pre-60S subunits at an early stage of assembly in the nucleolus, and is replaced by P0 in cytoplasmic pre-60S subunits and mature 80S ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 6">Associates with the pre-60S ribosomal particle.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Nucleus</location>
            <location evidence="6">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77273.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321692.2">
        <property type="nucleotide sequence ID" value="XM_003321644.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JYZ8"/>
    <dbReference type="FunCoup" id="E3JYZ8">
        <property type="interactions" value="685"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JYZ8"/>
    <dbReference type="GeneID" id="10537818"/>
    <dbReference type="KEGG" id="pgr:PGTG_03229"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03229"/>
    <dbReference type="eggNOG" id="KOG0816">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071690_3_0_1"/>
    <dbReference type="InParanoid" id="E3JYZ8"/>
    <dbReference type="OrthoDB" id="10262308at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05796">
        <property type="entry name" value="Ribosomal_P0_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000003">
        <property type="entry name" value="Ribosome assembly factor mrt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1730:FF:000038">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033867">
        <property type="entry name" value="Mrt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051742">
        <property type="entry name" value="Ribosome_Assembly_uL10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45841:SF1">
        <property type="entry name" value="MRNA TURNOVER PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45841">
        <property type="entry name" value="MRNA TURNOVER PROTEIN 4 MRTO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="7">
        <location>
            <begin position="125"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU364039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP77273.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="235" mass="26383" checksum="77655EC8579C469C" modified="2012-04-18" version="2">MPKSKRQTIVHLSKTSKKTKEVKAKLIEQIKEASENFQFVWLFTVDHVRTAYLQDIRSSWKPSRIFMGRNAVMRLGLGSKEEDEHMPGLGAIGKLLEGDTGLLFTNEPPKVVVEWFDDYVKADYARKGNLATETVELPAGPVMIKEINDEPSVAAGALEPHLRALGLPTTLQSRIPTLSSPHVVCKEGEKLDTNQAGLLKTLGYQMAQFKIVLSHVWIKDRSTTFSIDQIRDQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3K0L3</accession>
    <name>E3K0L3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic elongation factor-2 kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03794</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77838.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322257.2">
        <property type="nucleotide sequence ID" value="XM_003322209.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K0L3"/>
    <dbReference type="GeneID" id="10540822"/>
    <dbReference type="KEGG" id="pgr:PGTG_03794"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03794"/>
    <dbReference type="HOGENOM" id="CLU_837121_0_0_1"/>
    <dbReference type="InParanoid" id="E3K0L3"/>
    <dbReference type="OrthoDB" id="2500644at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031037">
        <property type="term" value="P:myosin II filament disassembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051852">
        <property type="entry name" value="Alpha-type_PK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45992:SF2">
        <property type="entry name" value="EUKARYOTIC ELONGATION FACTOR 2 KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45992">
        <property type="entry name" value="EUKARYOTIC ELONGATION FACTOR 2 KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77838.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="332" mass="36882" checksum="63E6FD7561E45134" modified="2012-04-18" version="2">MFYKGLAKEARAQTKCDISTPKVRRASSSCTPDPKPSPEKRFVKAGVSVYIDDVLQKKTGIIPKVLEVDTKKPNFYEDFHHHLWGLFSDEILLKTEITYPPNPEQYTCLGTHNSKIEDPNILLDLARSGKTIKSASVIHLIYQHPMDAFPETEIPTSSRTNSKRKYSGDQPKASKAATWALGGKFSTKPARGATSLSSQVNTLSHPVGQSIVIKTDGWIPALQVHFYTVDEQPPSGESRQSFIINDYIRAVTFPLEIKVNTGKVLGEGSSRRSYAAQVCSLEQGKEVVNKWVAKLRYSDPTPNVANHASDAITYRGFALILAEFKDLITHHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3K0Y8</accession>
    <name>E3K0Y8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Muskelin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_03919</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP77963.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322382.2">
        <property type="nucleotide sequence ID" value="XM_003322334.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K0Y8"/>
    <dbReference type="FunCoup" id="E3K0Y8">
        <property type="interactions" value="493"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K0Y8"/>
    <dbReference type="GeneID" id="10541111"/>
    <dbReference type="KEGG" id="pgr:PGTG_03919"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03919"/>
    <dbReference type="eggNOG" id="KOG2437">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004210_1_0_1"/>
    <dbReference type="InParanoid" id="E3K0Y8"/>
    <dbReference type="OrthoDB" id="10052615at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052456">
        <property type="entry name" value="CTLH_complex_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010565">
        <property type="entry name" value="Muskelin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15526">
        <property type="entry name" value="MUSKELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15526:SF5">
        <property type="entry name" value="MUSKELIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06588">
        <property type="entry name" value="Muskelin_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Muskelin N-terminal" evidence="3">
        <location>
            <begin position="11"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="Muskelin N-terminal" evidence="3">
        <location>
            <begin position="235"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="130"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="899"/>
            <end position="937"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="139"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="170"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="223"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="916"/>
            <end position="925"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP77963.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="987" mass="111524" checksum="6BB3DD99EA6584B6" modified="2012-04-18" version="2">MDPSTFDHPHQLSYTIHSSSTCSPVYHPFNILEDAPTDPNSRWSGSGPATKSASAEATSLIKLPSKRKDYIIIDLGKPSIVRSITFGKFHRPHPCNLKLFRIWGSLAPGEGKGDFRALLGNQTIDQHLNPASCTNPLNSHHHHHLHHHQHHQANHHQSIFKNQPLAPLPSSSSSSSTTTTNQTAAAATTTTNNNLEQSHSINPIQIDPHDLIKPINQSPPHPIRARGPTHRTGPRMELLAQGELKNDTNPETVPLRWKNDRVTWFKSSEVHRASSDSTQTVIVPVRYIKIEPLSATGSNYNCSIWHISVTGHDEDHIMSPCLEIYEKWRASAALRLCLKHLRQTGHLEAFQALINSATNPDQSPLLSSDHLPFKIPPISDAEQIVINAALDNLFDPWALAEAPTVTCHQILPRSTPASGLPCPRGGHQMCIDSKRRKIWLFGGFDGVTDLGDLWTIDPPRPTVHSHGNTQWQLISRDVKLDNGPMNRSCHKMVLDEMTGDLYVLGRYTERGRPPTHQPSDFPQNPSSHELWDNNGRIGQWECLSQDTHLENGPQLIFDHQMVLDSDRRKIYVFGGKIIQPADSLSQETASSSSRVTLNQYSGLFEYDLTTRTWVQLMLIDLLDHQVHIPSRMGHSLILDKKRQCLWVLTGERDHHYYSDLWKYDITHRRAELIVADYTYGGKNQQTSDVFANSSGSAPEAGFSQRTTFDPEADEWHMFCGLCRDRGSGEAATKPNSKSEMMSSEFWRWRIDEGRWTKVTMKAQVDGTETPPPRFAHQMVFDDLTKTHYVFGGNPADNDNEPTRLGDFWKLQLIQPTRREALRRCLFALRRQHFIEMCEKETDTVSALLYLQNDLSSVTNHLDEREAKLFRACTSHLLAGPGPRVPLLEEDAAQEDDLEGRYGEEDGEDCGMTARQKSSEDQDHADVGPGAQNGNGLGTEIIDRYRKIRDQRTQLFQSLLKFFPPSMTEPEESLMDLIDVWGDRESFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3K1L0</accession>
    <name>E3K1L0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP78185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322604.1">
        <property type="nucleotide sequence ID" value="XM_003322556.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K1L0"/>
    <dbReference type="STRING" id="418459.E3K1L0"/>
    <dbReference type="GeneID" id="10541815"/>
    <dbReference type="KEGG" id="pgr:PGTG_04141"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04141"/>
    <dbReference type="HOGENOM" id="CLU_111676_0_0_1"/>
    <dbReference type="InParanoid" id="E3K1L0"/>
    <dbReference type="OMA" id="RIENTHR"/>
    <dbReference type="OrthoDB" id="76487at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023:SF4">
        <property type="entry name" value="GLYCINE-RICH PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="128" mass="14275" checksum="8A4CBFF203028D21" modified="2011-01-11" version="1">MPATATPNPSAPAPATSEGPADSNKPHRAPNFGHEEDEQLAKSWVVISEDAIRSNNQKLEDFWARVLEDFNKYTPGPRRDASGISIRWKALQKACLKFCAIYDQIKQNPASGSSPDDWILDARQAYYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K1N4</accession>
    <name>E3K1N4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP78209.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322628.2">
        <property type="nucleotide sequence ID" value="XM_003322580.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K1N4"/>
    <dbReference type="GeneID" id="10541999"/>
    <dbReference type="KEGG" id="pgr:PGTG_04165"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04165"/>
    <dbReference type="HOGENOM" id="CLU_033090_1_2_1"/>
    <dbReference type="InParanoid" id="E3K1N4"/>
    <dbReference type="OrthoDB" id="2508213at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="291"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78209.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="488" mass="56325" checksum="CA9FC9D90719A499" modified="2012-04-18" version="2">MNRGTWGSLQCCTIDQTTMLDHETRPNSVTDLIIELLNSIPRRYRRISTDIDDEESERPWDIDDDTVWLHANWRYARGEALSIGEIDNLNGLLLQMQNSYLPSLQQQLGDLLESLNLDDLPGRFPNPKLPDVLEILFRLVHTVDQINHFVQSIAPIVVDPLHSYKKNDRDYGGLKVYRATELIDEMNDLMHQHVQTLFNHLARFIRALPKNPLISSYVESELTLHSSNLINAATAQTLRAIDRLIEWSKKSDFSILQDDCRRFAIKLDPILEDLISRIIVKPRIEKLLEFESDDQAEPANEHEDIRSDIDSNDHHQDNRSSTSHLTDAYEWDIPLSVQVIRLVKAPRSFIKILRIFFNKLSDTPISKAPFTIGTQLCSDEITSIDHEIKCLDNGFQVFLKVIYEIDENPMQMAKVIHLGILHDNLCRRFDSSIALLSFHLIPLSETQYDLPKSVNLCKMWFLTLREHFCMASESFNSAINRVKCAIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3K1V2</accession>
    <name>E3K1V2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Sister chromatid cohesion protein DCC1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_04233</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DCC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP78277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322696.1">
        <property type="nucleotide sequence ID" value="XM_003322648.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K1V2"/>
    <dbReference type="FunCoup" id="E3K1V2">
        <property type="interactions" value="281"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K1V2"/>
    <dbReference type="GeneID" id="10542069"/>
    <dbReference type="KEGG" id="pgr:PGTG_04233"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04233"/>
    <dbReference type="eggNOG" id="KOG0798">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034504_0_0_1"/>
    <dbReference type="InParanoid" id="E3K1V2"/>
    <dbReference type="OMA" id="DSESWPF"/>
    <dbReference type="OrthoDB" id="276989at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000775">
        <property type="term" value="C:chromosome, centromeric region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034088">
        <property type="term" value="P:maintenance of mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019128">
        <property type="entry name" value="Dcc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13395:SF6">
        <property type="entry name" value="SISTER CHROMATID COHESION PROTEIN DCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13395">
        <property type="entry name" value="SISTER CHROMATID COHESION PROTEIN DCC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09724">
        <property type="entry name" value="Dcc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP78277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="449" mass="50548" checksum="1D570F38EA2C460D" modified="2011-01-11" version="1">MTLTSSSQEPHIIRFASPNHHQEIFNSNIHPTDDDELVNTSYLLLDLPPELLEPVKKQLQGHDTGNADLQMELRGRNDDEVVLTTPDKTFQLRTVQNSNSVMICGTGSLDDENQKLGLTVLKVIHETIELSDVTSFPGSRLDRIKELLQPHLYSGETNEAVRRAEDSDSTDAPIPVSLSFLSQQIRASDSEIQDYLRQLHVIELGGSLRMISLGYLSTILSGILKSLDELGVGPHEQFAVGPVVDIITSRLDIHQAALLQILHKFSIDFKIHEPYDSFDPNQNASLKLQEVVNSIGLAQLLCIKPTKIESNHNPKDHRYIQKMNVESFLATWTSKLPEGLSEHCSIESLSSHSILSNDQKNIIVIPIDKLSLEPKMRMSQLFEIQPKWKIDQIERFLTPITGGGMKKKFDELVLKFCRKIKEPSASQSKQNSKNSNAPETVWLTARNKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3K351</accession>
    <name>E3K351_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">U3 small nucleolar RNA-associated protein 10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_04864</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in nucleolar processing of pre-18S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the ribosomal small subunit (SSU) processome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
            <location evidence="1 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the HEATR1/UTP10 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78908.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323327.2">
        <property type="nucleotide sequence ID" value="XM_003323279.2"/>
    </dbReference>
    <dbReference type="FunCoup" id="E3K351">
        <property type="interactions" value="681"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K351"/>
    <dbReference type="GeneID" id="10537015"/>
    <dbReference type="KEGG" id="pgr:PGTG_04864"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04864"/>
    <dbReference type="eggNOG" id="KOG1837">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001128_0_0_1"/>
    <dbReference type="InParanoid" id="E3K351"/>
    <dbReference type="OrthoDB" id="31183at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034455">
        <property type="term" value="C:t-UTP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045943">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012954">
        <property type="entry name" value="BP28_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056473">
        <property type="entry name" value="HEAT_Utp10/HEAT1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040191">
        <property type="entry name" value="UTP10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13457">
        <property type="entry name" value="BAP28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13457:SF1">
        <property type="entry name" value="HEAT REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08146">
        <property type="entry name" value="BP28CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23243">
        <property type="entry name" value="HEAT_HEATR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01036">
        <property type="entry name" value="BP28CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 8" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="BP28 C-terminal" evidence="9">
        <location>
            <begin position="1526"/>
            <end position="1673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU367065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP78908.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1815" mass="199020" checksum="9682D680C15E44CD" modified="2012-04-18" version="2">MMQTLIVIFQNHTEEGEIRLSSKSCHAICRFFKVGELLRSMCETYEVSAFWKSLIQGLVKCTLKNEVVAEELVVKLAQIYDLPTGLVETFITESLSVLLPMEVPHRAALRPLGILFQRHPDLVEQIAQSLLSTSEGNAKESIPRILRLLSGDVPLTTDQCDPSVVGLSSSSAIVRQDSLKAIMEQMNANALDGSGSPSDLLNSALKTILKDPEPTTSELLLSFPQALLKAVKVDDIMSAATSILCCHQTPRSTLKHWLSFLMGPFLHQYPDMAIRVSEEIILARLFWTKSTAKSTSVFWTHVHDKNIWNGTILEGVWPAPVPGDSVESRITANESLLDGVGQNILKRGDSAVIGLLQKLRKDNKLESPTARLVPLLCLWSITSGLSGGVAGALMSALIDYMTVQTDSGGFNRWVNMDIGDAGTDRSILEMFYSKTSSEKLFSRLTASILTKAATQADKLKRPEYCWLEPGILKAMDPISSTSIVSQPSDIASFCFKLYLFSHSASHTTQQSLARMISGTLRHNILKQEWLTFLARVWTSSLFNQSFRVVALLDAEAEIKALTACSQNSDKVTLLDYQILIPSVMIALIDPCKPVRSAALSLTAALNTYLSSVDLAKKTSGLQPEQSIYAYDRFYGSRASADLQYLSIPDSTRLIALLHQSKTEISFDGLSRIRSILKNLEDSPTGASNRKRQAEGLRHRILCFFMKVIDCWNDFEGRVKLLKCISYVNDPVRIKSVAPLIDEMSSGAANSITDNNLPKEALAEYAALLFQTYDYPGKVFATGKDSTALDTLVSALCCSPANDAQLAIREAAKNKLQSGLIATLSSSQKQSILRRILVLASSSRDDVSFYVNTLRCLPLDSESVIATLSVISREISSNNVRSNKRTKTGKGSVGQATGGAPDLSELVTLLETISVEKLKHTFQLFAAFLDTLAILLATHTTHEVDIHFSAQLLISYLAIVAPDLDEAEFKSDGLPLSSIVDYMRISLDPHISHKIILLLADLARLCPELTCQSMMPIFTFVGAHVIQRDDAFSARVVDKAIQALSPPMVKASSASGSSRIELILSLRELLLMFVGARNHIPKHRRTRLFVRLIEVLGEYQFLGALLILMIDANIDEDNATSSFELPLAIWSSFPGPLGVAAIAHIVAEVETLLGLSASGEPGVLPAKTQQTEVDGDGDQIIEDESQELNTTRIQALMKFMSEALGSKSLKSKLDSARSTNNSDSDQVLAFLMMQILELCQPKPEQTESDQLQDARDIALRAAKLVSLEFFATAMLPKLQNHDSKLVFCTLDILRARLSSVKASQRPEIEMCVMEAIKICTNRIQGSFAISSGDAKVPLLDAIETLGDIAVDATASEQSLLSKAYGSLLSIPPSSNDSKVTSAALSALVKLCRLLGSRLIPSIGKTISMCVELVQKPQGFESDTAAAELRALAFRLVEATVKTLPAFLTPHMPIILRLITTPIMSKPGLQETVDVNQASLIRCLTKTVPLHNLGSVVSAYWSNLEGSSNVALALVEILLRAIKYAKVPEVIKESKAIFDFLLQIFDLRRQNGGNIPENDMMKIESLASSTFLSLVLKINDETLKPLLFRLIDWATITLTKEKNQPDVARSIALYKVFGALLRHLQTLAVSYFTHLISHTVTILNGFAEGKQSDFQLWTEVTSTLEQTLIHDTEGFWSATALTKITMPVINQIKLGSKFMVHQEYSSRVKSLIGKLAQKVSSHDTLLKVLNSGILQEIKARDDEEEDNTINVKLISLGVLEEIWKEIGAALVPFVPETIGGCLIEALEESHGGIDQAAKKVVKRIELEIGESIEGYLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3G8</accession>
    <name>E3K3G8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04981</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP79025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323444.1">
        <property type="nucleotide sequence ID" value="XM_003323396.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3G8"/>
    <dbReference type="GeneID" id="10530846"/>
    <dbReference type="KEGG" id="pgr:PGTG_04981"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04981"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043573_0_0_1"/>
    <dbReference type="InParanoid" id="E3K3G8"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="173"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="187"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="263"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="307"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="369" mass="39511" checksum="CA449724A1F04FF0" modified="2011-01-11" version="1">MSSSSVRRSTSSGQLNTINPIYHNQDYSLPTTPSLIVQFPSTFIFDHHQPQELDFLSLNNSPFTASNNKPHLNSNIDPSLINPSPKQTSKIHHHHHHHESDKHQSLRGFLKDPSSDPALHTPSPSLRSPLIDQDPFTGVVGTVSMSDYSSHLSSLDLFDRLTTDDWERGLTSSTPISASHLSGHHPSSSTSNHQRSSSLPPPAIPESPSSLSASARLLLPKSSDHPRFTSYLDGLDLHLSASSSFLFDDDAFLPAYVIPTSAATATSSSSSSSSSASSASSSSTFSSSPSSTPSTRLDNPLDSIHSTPDHHLELFPGLEEDKGLPIDPAIIDPFGPPSSSSTPLPFQKDGLIHPSLSPAGPIDLTKNST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3M1</accession>
    <name>E3K3M1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_05034</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP79078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323497.1">
        <property type="nucleotide sequence ID" value="XM_003323449.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3M1"/>
    <dbReference type="GeneID" id="10530899"/>
    <dbReference type="KEGG" id="pgr:PGTG_05034"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05034"/>
    <dbReference type="HOGENOM" id="CLU_1525911_0_0_1"/>
    <dbReference type="InParanoid" id="E3K3M1"/>
    <dbReference type="OrthoDB" id="10491120at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP79078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="176" mass="18555" checksum="765D603DEACCC9BC" modified="2011-01-11" version="1">MGPMETTTQALNLEQTSVQGLKPEGHHPRFEFWPMALKIPSWCWGSSPAPQPVSSSELGSVCLSSGLKFSSTNHQLTSLLASNQPKPTQPAHPEPLRDTQAPHGIALALREGSEPAVGLTCDAGLTVRDVVGLTSNAGLTVASDPNTIIPTCTSAEVGGHWSDIGWPKRSELGLLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3K486</accession>
    <name>E3K486_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Phosphoglucomutase-3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_05417</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79096.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323515.2">
        <property type="nucleotide sequence ID" value="XM_003323467.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K486"/>
    <dbReference type="FunCoup" id="E3K486">
        <property type="interactions" value="189"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K486"/>
    <dbReference type="GeneID" id="10530919"/>
    <dbReference type="KEGG" id="pgr:PGTG_05417"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05417"/>
    <dbReference type="eggNOG" id="KOG1220">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016950_0_1_1"/>
    <dbReference type="InParanoid" id="E3K486"/>
    <dbReference type="OrthoDB" id="8300170at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008973">
        <property type="term" value="F:phosphopentomutase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006006">
        <property type="term" value="P:glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05799">
        <property type="entry name" value="PGM2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000035">
        <property type="entry name" value="Pgm3p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45745:SF1">
        <property type="entry name" value="PHOSPHOGLUCOMUTASE 2B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45745">
        <property type="entry name" value="PHOSPHOMANNOMUTASE 45A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0313">Glucose metabolism</keyword>
    <keyword evidence="9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="11">
        <location>
            <begin position="50"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="12">
        <location>
            <begin position="219"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="13">
        <location>
            <begin position="343"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EFP79096.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="615" mass="68129" checksum="352BCA277E6E121E" modified="2012-04-18" version="2">MASSRSSVPLETLVQDWLRLDRNPTTRKEIEQLQQDKNHDELASRLCQRIKFGTAGLRAHMSAGFSRMNDVTVIQASQGLAAYLIKDIPNATQKGIIVGHDHRHNSHRFARLAVTAFLRRGFRCYLLDGSVATPMVPFGVKYLGAAAGVMVTASHNPAADNGYKLYYSNGVQIISPHDKGIAASIEQNLEIDEEAWSTEPTAQISEALLVNRTQEIQDAYFKAISGLVRHSKLENSKTPLKFIYTPMHGVGYPFATRACVDTAGFPSHAWLPVEAQKDPNPDFPTVKFPNPEEKGALDMAMSLGNKTVEADRAQKVMILANDPDADRFCAAEWTGSNWKTFSGDQIGAVFACWTLQNFKKLGTPLDRIAMLSSTVSSHLVAQIARQEGFKFKETLTGFKNIGNEALNLEKEGYKVLFAYEEALGYMFETGIFDKDGLASIVVWAELATELAQRGSSVAEYLENIYKTYGYFATNNGYFVCKDPSAVKAKFDSLRFGEQSEGKPDPSSRSMMLDQLRYPSALAGYPITRIRDLTIAYDDSYPPQCLPDLPSSSDEMITFYFGGEAGEVIATLRTSGTESWKLKYYVEGRAEDQLTAQKKVDQIVAALGEEWGFSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3K487</accession>
    <name>E3K487_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4219 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_05418</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
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    </dbReference>
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        <property type="term" value="P:phospholipid transport"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027532">
        <property type="entry name" value="Mdm12"/>
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        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
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    </dbReference>
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        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="204"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP79097.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="248" mass="27976" checksum="98081B708C0D6FC0" modified="2012-04-18" version="2">MSNTRNAPLLLNESNYTTWSFLMIAKLSKIGALDVVQGLIQKPVLVDIKKPTEEFVVYSETNRVAYIEIIEHLDTNHLAYIAQILNDDDSFDGHSVWQILKKKYAGDDYIAKDSALEKFLDLEYHGSTTEFISEIRAANHRLVTAKVGLDDLVKTSIILRKLPSEFKSFRTVVSVGCPLDSVATMLSRLEKHAAQNHLDRSSDSRSVPTATKPVTRSRSASKSTLNNDHQKHQSLRYHRNPSQLLKPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3K4C1</accession>
    <name>E3K4C1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05720</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
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            <taxon>Pucciniomycetes</taxon>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
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    <dbReference type="KEGG" id="pgr:PGTG_05720"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05720"/>
    <dbReference type="HOGENOM" id="CLU_2400766_0_0_1"/>
    <dbReference type="InParanoid" id="E3K4C1"/>
    <dbReference type="OrthoDB" id="10441728at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="93" mass="10392" checksum="D884F2B56491F475" modified="2011-01-11" version="1">MCPRVRLRFLEHSIGPIPRSLFNQRPRTLLNLDTMRFLCVLFCLFISMFSQAAEIPPAEPSSEKDPHTTENAPPTRQILGEGGRPAKYGPLGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3K530</accession>
    <name>E3K530_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_05666</name>
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    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003323716.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="10531169"/>
    <dbReference type="KEGG" id="pgr:PGTG_05666"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05666"/>
    <dbReference type="HOGENOM" id="CLU_2185250_0_0_1"/>
    <dbReference type="InParanoid" id="E3K530"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP79345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="109" mass="11993" checksum="0535926B0A382F45" modified="2011-01-11" version="1">MSCVRVPRKGMGVGDIIDTGGRRPAGTLRQAIESTAWRDLVKLAPFDGCVQEYWDVNESCQSNSTGFNCADLTKKHVLFPDSLRMKLVEACRNPTSAAWRAMGHTFAST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3K6N8</accession>
    <name>E3K6N8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_05259</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EFP80034.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324453.2">
        <property type="nucleotide sequence ID" value="XM_003324405.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K6N8"/>
    <dbReference type="GeneID" id="10534051"/>
    <dbReference type="KEGG" id="pgr:PGTG_05259"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05259"/>
    <dbReference type="eggNOG" id="ENOG502S8Y2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1448381_0_0_1"/>
    <dbReference type="InParanoid" id="E3K6N8"/>
    <dbReference type="OrthoDB" id="2442555at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48472">
        <property type="entry name" value="TC1-LIKE TRANSPOSASE DDE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48472:SF1">
        <property type="entry name" value="TC1-LIKE TRANSPOSASE DDE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP80034.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="187" mass="21208" checksum="B270523ADC450F5C" modified="2012-04-18" version="2">MCCDWAHAHRLDPNLYLFTAPNSTHNNTISTSLPHPNQPTTTSDDIFLNGLLVARAGYQDCNRLNGASGIETRCVIKDPETYKARSRPASLSRDECLFMVCLVQSEPGLFLDEIQERLYDSSGVFLSVEGVHRNLVEQLSITLKKPETKNCWKSLVEKYSFVEKMEFFPADFLVFTDESSFCDKDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>E3K7P9</accession>
    <name>E3K7P9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">NEDD8-conjugating enzyme UBC12</fullName>
            <ecNumber evidence="7">2.3.2.34</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">NEDD8-conjugating enzyme Ubc12</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">RUB1-conjugating enzyme</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Ubiquitin carrier protein 12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGTG_06303</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine + [E2 NEDD8-conjugating enzyme]-L-cysteine = [E1 NEDD8-activating enzyme]-L-cysteine + [E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]-yl-L-cysteine.</text>
            <dbReference type="EC" id="2.3.2.34"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein neddylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.34" evidence="7"/>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80347.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324766.2">
        <property type="nucleotide sequence ID" value="XM_003324718.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K7P9"/>
    <dbReference type="FunCoup" id="E3K7P9">
        <property type="interactions" value="882"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K7P9"/>
    <dbReference type="GeneID" id="10539355"/>
    <dbReference type="KEGG" id="pgr:PGTG_06303"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06303"/>
    <dbReference type="eggNOG" id="KOG0420">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_6_0_1"/>
    <dbReference type="InParanoid" id="E3K7P9"/>
    <dbReference type="OrthoDB" id="10249039at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061654">
        <property type="term" value="F:NEDD8 conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019788">
        <property type="term" value="F:NEDD8 transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045116">
        <property type="term" value="P:protein neddylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23794">
        <property type="entry name" value="UBCc_UBE2F_UBE2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000005">
        <property type="entry name" value="NEDD8-conjugating enzyme Ubc12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="14">
        <location>
            <begin position="105"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="12">
        <location>
            <position position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00043698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EFP80347.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="262" mass="29372" checksum="C9AABF723F37E8B6" modified="2012-04-18" version="2">MPDLSGSIPSPDPVCGVSGSCRICGVRIGTLGCLHSKSNIPYKKDTQDPPGELQHSPFPQAHSHPIRLVHLYDPYHPYSEPKMIKIWSLKKAESDAAKKKPKTSAAQIRVQKDLTELELPSTMTTHFPDPTDLLNFTLTIKPDEGMYKGGSFIFNFVINLNYPHEPPKVKCTQKIYHPNVDIEGNVCLNILREDWKPVLNLNAVIVGLQYLFLEPNADDPLNKEAANDLRNDRIAFEHNVRSSMNGRSIRGTSYDVVTTKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3K8Y5</accession>
    <name>E3K8Y5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_06760</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325223.1">
        <property type="nucleotide sequence ID" value="XM_003325175.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K8Y5"/>
    <dbReference type="GeneID" id="10539825"/>
    <dbReference type="KEGG" id="pgr:PGTG_06760"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06760"/>
    <dbReference type="HOGENOM" id="CLU_1769031_0_0_1"/>
    <dbReference type="InParanoid" id="E3K8Y5"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP80804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="147" mass="16605" checksum="39D7ED9C95B2917C" modified="2011-01-11" version="1">MVANGKEVDPLSDEEDSDLEIDYTDIKEQFRPKFKRGEVLARLLLDSKPRSQDKASPLKGLWSSDWWRLMMRLSEVSRLAEVGGMVDPDCLAELALQSVSRRTGSSGPVTGLPPDINIWEDTVHCYSENMCKNENRKFPQGKTAFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3K948</accession>
    <name>E3K948_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DUF202 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_06640</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325103.1">
        <property type="nucleotide sequence ID" value="XM_003325055.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K948"/>
    <dbReference type="GeneID" id="10539701"/>
    <dbReference type="KEGG" id="pgr:PGTG_06640"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06640"/>
    <dbReference type="HOGENOM" id="CLU_1603551_0_0_1"/>
    <dbReference type="InParanoid" id="E3K948"/>
    <dbReference type="OMA" id="HACCAPI"/>
    <dbReference type="OrthoDB" id="5525680at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003807">
        <property type="entry name" value="DUF202"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052053">
        <property type="entry name" value="IM_YidH-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34187:SF2">
        <property type="entry name" value="DUF202 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34187">
        <property type="entry name" value="FGR18P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02656">
        <property type="entry name" value="DUF202"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="domain" description="DUF202" evidence="7">
        <location>
            <begin position="46"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP80684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="166" mass="18668" checksum="02823E9E36A32AFE" modified="2011-01-11" version="1">MNREAQSHSAATHLNPSPVHPISASGIISSCFGSPLVIPNEGSTARDHLGRDRTWMQWIRLSTLLVLVSLATCISFPFDFDDHDDETQKNNHHHRQHLNPFAVTFLVLSVLLPLLGFFEYLSTHHAMSMKRGFVQTGFFSGFMITATCLVLSLFTFVLFFDGFLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>E3K9E4</accession>
    <name>E3K9E4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_07135</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP80883.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325302.2">
        <property type="nucleotide sequence ID" value="XM_003325254.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K9E4"/>
    <dbReference type="GeneID" id="10539907"/>
    <dbReference type="KEGG" id="pgr:PGTG_07135"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07135"/>
    <dbReference type="HOGENOM" id="CLU_2307417_0_0_1"/>
    <dbReference type="InParanoid" id="E3K9E4"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP80883.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="100" mass="11132" checksum="CFFAEA6366715B1E" modified="2012-04-18" version="2">MQEERVAGLEVAERQAPNADRWQLYSNFRLGRSPSPQTPKRLPIAVAVIVTDYLNPTAPDLPSVAGRNSAKDLSRAKRKSPWGRLWPGGANVRIESKVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAT2</accession>
    <name>E3KAT2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4219 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_06962</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP81341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003325712.2"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06962"/>
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    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
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        <property type="project" value="InterPro"/>
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        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
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    </dbReference>
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        <property type="term" value="P:phospholipid transport"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027532">
        <property type="entry name" value="Mdm12"/>
    </dbReference>
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        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
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    </dbReference>
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        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="254"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP81341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="311" mass="34744" checksum="6263EFA1A1AF931E" modified="2011-01-11" version="1">MTPSRTAPLLLNNSNYSTWSFLMTAKLSKIGSLGVVLGSVQKPVIEKDKPPSEKSIAYEETNRMAYIEIIEHLDDSHLAYVSQTLPEEASFCGYSVWHILKKKYAGDDYVSKDLALEKFLDLDYHGSPSEFITEVRAANHLLVSSKIGLDDQVKTSIILRKLPSEFQSFRTVLSVGCAGDSVALMLTRLEKYAAQNHLDRASTVPSQQALLTTDDKFYMCPHCKKGFTICSHCDKAGHREAICFEKHPDRRPTKSSPSTSSSKTTTSAQAHLAFSTPHGFTPEQVESERNFQAMLANPEFKKLNINAEYRL</sequence>
</entry>
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    <accession>E3KBI7</accession>
    <name>E3KBI7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_07934</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81685.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003326056.2"/>
    </dbReference>
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    <dbReference type="KEGG" id="pgr:PGTG_07934"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07934"/>
    <dbReference type="HOGENOM" id="CLU_837114_0_0_1"/>
    <dbReference type="InParanoid" id="E3KBI7"/>
    <dbReference type="OrthoDB" id="10292206at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173410" evidence="2">
        <location>
            <begin position="25"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP81685.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="332" mass="37157" checksum="0963DB011E0638FD" modified="2012-04-18" version="2">MYLPRECTMSRLKFFMGFALLILAQRYDAAMDKSWRDVVYNVAPEQHMMQIEQGPQLLPLFPTRFSRTQTTGDSAQTGDEHQSRKRPTGAIRESFKIGKVQGPYHPTAKRQQVDLELRLAVSRPTTAALSSLPPSEIGTNDKPIPSEIIGVSGSESEKNFRHPTSAIKNARPESSRAPSCLARKSPSSNVRSAPELIDVSSRDMGSFSKQSPINDRRLTTARLEVSNQLQSGKFATDSQGLINQDSTRNANDDLTRKRTDEVPREFLLENMTSKVWPWISLIGNRVHHLISSELKTYVDPLIPHLTQACLSHCARSPSGIADIRFIFKNNQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCC4</accession>
    <name>E3KCC4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_08280</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EFP82031.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326450.2">
        <property type="nucleotide sequence ID" value="XM_003326402.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCC4"/>
    <dbReference type="STRING" id="418459.E3KCC4"/>
    <dbReference type="GeneID" id="10534399"/>
    <dbReference type="KEGG" id="pgr:PGTG_08280"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08280"/>
    <dbReference type="HOGENOM" id="CLU_114370_0_0_1"/>
    <dbReference type="InParanoid" id="E3KCC4"/>
    <dbReference type="OrthoDB" id="2510161at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047499">
        <property type="term" value="F:calcium-independent phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019369">
        <property type="term" value="P:arachidonate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24185">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24185:SF1">
        <property type="entry name" value="CALCIUM-INDEPENDENT PHOSPHOLIPASE A2-GAMMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="2" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP82031.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="209" mass="22422" checksum="E74CDE3E0EE72567" modified="2012-04-18" version="2" fragment="single">GGRCSVAQARRATSAAPVFFKAARVDELSLNKPLMEAVKEAQTLCPGQRIDCLISLGAGKGLHNGSGSIAHACSKMGESSKRVARKVKARAAQEGWSASYFRASVKGLKNKDNQGEWENPDWVKAGTIKYLFIDARSQFVRHLDNLINGNPSRPQSSSLGGIPPEKLGWDEYPLVGRGGGYPRVSARIHARASGYPPADADVGADVRFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDF8</accession>
    <name>E3KDF8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_08350</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82394.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326813.2">
        <property type="nucleotide sequence ID" value="XM_003326765.2"/>
    </dbReference>
    <dbReference type="FunCoup" id="E3KDF8">
        <property type="interactions" value="465"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KDF8"/>
    <dbReference type="GeneID" id="10529771"/>
    <dbReference type="KEGG" id="pgr:PGTG_08350"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08350"/>
    <dbReference type="eggNOG" id="KOG1870">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001060_2_0_1"/>
    <dbReference type="InParanoid" id="E3KDF8"/>
    <dbReference type="OrthoDB" id="292964at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2230.10">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035927">
        <property type="entry name" value="DUSP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006615">
        <property type="entry name" value="Pept_C19_DUSP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050185">
        <property type="entry name" value="Ub_carboxyl-term_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21646:SF24">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06337">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00695">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143791">
        <property type="entry name" value="DUSP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51283">
        <property type="entry name" value="DUSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="DUSP" evidence="10">
        <location>
            <begin position="11"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="345"/>
            <end position="1184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="267"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="416"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="802"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="839"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1045"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1080"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1198"/>
            <end position="1410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="844"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1086"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1110"/>
            <end position="1121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1199"/>
            <end position="1218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1256"/>
            <end position="1267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1310"/>
            <end position="1336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1389"/>
            <end position="1402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP82394.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1410" mass="155926" checksum="8E39D77289A2D2AB" modified="2012-04-18" version="2">MVKEVEMDEIPSPQEQARLYHSFRSIRMATDQTWYLIHQQWFNTWSSSIGIATDDSTAAGTTTTTTDIPSIDNSDLLDHENQLRFGISIGIDCEIIPEEFWNLLVKWYGLKQPTHAIPRSVIAPSGPSSESVEFYPPSFIFHLILPSSNLEELESEQQYDLNLIRQLPNLVIQKRFSIGNSIGDLKRELTSNLLPAGLLTRSFKLWSIHSPPSNPNQLILSVSEFGNLETQLVEPSSGDAADLNEALLTDPVNVLAIEQQDQEGHWLINPKSAPPSTPPQLQDDDPTSSGPVFGGQEWLDRMEKMNQANLLDPKFSITSPNSDLSLVAFNSAQSAFKARGIRGLVGLTNLGNTCFMNSALQCLSNCPELKTYFLSRVYINELNRTNPLGMGGKVAETFGQLIERMWSSEYEVSRRIQGSTSGTNGPTSYGGSSHQSISPREFKSIVGRFNSLFLGYGQQDSQELLTFLLDALHEDLNRVKVKPFDEIPDYDDQNSLNDPLLEAQKILKLAKTCWNLYRRRNDSVIVDLFQGQYKSTLICPDCNKVAIKFDEIMYLTLQLPINKKFRGTIYFVPLDLSKPRIKVNYQINKDSTIRQLKQHIGQILCVDPDRMAAIEDWSGKPWKIWHDTDSLEGVMERDVINVFETPLPFAALKSGIYHTNGEEEEVPDLLIPVVSYRPAERMNMGSSTSIHKPSSTAFGVFFVARISAKDRLSTRAVYDAVAREYARYTSQAEELFERNEDEDVVMNEPTAEIPPSSSSQPTLHAVPNLFKISVPKTPCSTVFPVSNAMGNATIELEERVKLSASKAPPGPAPVSGVNEDANGSNGAVFEMEENEDLYEDKAAAPEPRSSSSPSHSSQTGPVVFEGDYFECEWTPSAMGHFFGLDKNLPNSKNWIDPPVIEDPELVASRVAEQNKKSGAAGELTIEECFKDFSKPEKLGSEDKWYCPRCKNHVQATKQMQIWKVPDILVVHFKRFSSARTSYGRSSKVDNFVDFPIQGLDLSNEVEGIKVVRELKKHQRNLKASSRDQPVADQDQDHKNLPQILVDDHLPSSSNPSDHNNNDKVGGEGKEIDAILEDQNTELDDGKEERQQQQQQDGDRERDGHENPQQEGEDDEDEEEEESLIYDLFAVDNHFGGLGGGHYTAFAKNEEDGKWHNYDDSHVTEVNSPERVKSSAAYLLFYRRRTTRKLGLKTHGIVSSAMQSRDISARSSSQGNSSHMADDDEDGSRAAIKTGAGARHAAMASSSSNGLERPTSQEEEEEVDELDEGVGAGVGAVGRGSSIGSVGREDGSLRSESPFSDLRPNLSRPISSSSSSPASHSPDASHSASPASHSSSSELVPSLPLGHHSLLDSSDPILSHHSANHRDSSNLPAVGPSPADDRHHVAPSAAPIPLPPVPPPPYKPHSSASSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3KED8</accession>
    <name>E3KED8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 14">Uroporphyrinogen decarboxylase</fullName>
            <ecNumber evidence="5 14">4.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PGTG_08867</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the sequential decarboxylation of the four acetate side chains of uroporphyrinogen to form coproporphyrinogen and participates in the fifth step in the heme biosynthetic pathway. Isomer I or isomer III of uroporphyrinogen may serve as substrate, but only coproporphyrinogen III can ultimately be converted to heme. In vitro also decarboxylates pentacarboxylate porphyrinogen I.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>uroporphyrinogen I + 4 H(+) = coproporphyrinogen I + 4 CO2</text>
            <dbReference type="Rhea" id="RHEA:31239"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:62626"/>
            <dbReference type="ChEBI" id="CHEBI:62631"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:31240"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>uroporphyrinogen III + 4 H(+) = coproporphyrinogen III + 4 CO2</text>
            <dbReference type="Rhea" id="RHEA:19865"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57308"/>
            <dbReference type="ChEBI" id="CHEBI:57309"/>
            <dbReference type="EC" id="4.1.1.37"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="13">
            <dbReference type="Rhea" id="RHEA:19866"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the uroporphyrinogen decarboxylase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.37" evidence="5 14"/>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327090.1">
        <property type="nucleotide sequence ID" value="XM_003327042.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KED8"/>
    <dbReference type="FunCoup" id="E3KED8">
        <property type="interactions" value="554"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KED8"/>
    <dbReference type="GeneID" id="10530054"/>
    <dbReference type="KEGG" id="pgr:PGTG_08867"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08867"/>
    <dbReference type="eggNOG" id="KOG2872">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040933_0_0_1"/>
    <dbReference type="InParanoid" id="E3KED8"/>
    <dbReference type="OMA" id="LWLMRQA"/>
    <dbReference type="OrthoDB" id="339900at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00321"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004853">
        <property type="term" value="F:uroporphyrinogen decarboxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00717">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.210:FF:000008">
        <property type="entry name" value="Uroporphyrinogen decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006361">
        <property type="entry name" value="Uroporphyrinogen_deCO2ase_HemE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000257">
        <property type="entry name" value="Uroporphyrinogen_deCOase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01464">
        <property type="entry name" value="hemE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21091">
        <property type="entry name" value="METHYLTETRAHYDROFOLATE:HOMOCYSTEINE METHYLTRANSFERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21091:SF169">
        <property type="entry name" value="UROPORPHYRINOGEN DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01208">
        <property type="entry name" value="URO-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="UROD/MetE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00906">
        <property type="entry name" value="UROD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 14" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="9 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="10 14" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uroporphyrinogen decarboxylase (URO-D)" evidence="16">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00014308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045708"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU000554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EFP82671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="387" mass="43136" checksum="69E501D914B080BA" modified="2011-01-11" version="1">MSKETVTNNSPTNDLLLRAARGEKTERSPVWVMRQAGRYLPEFRKLRESHGFFEICRTPELATEVTMQPIKRYEGLLDGAIIFSDILVIPQALEMEVIMNPGPIFPNPISSKEQMEEMINKKISPEELKEKLNYVYEAIKLTTLELQNRIPLFGFVGAPWTLFAYMTSGGGGSGLNASKATSFDTAKNWIQVHPELSARLLEKLALVCALHLVNQIEAGCQIVQVFDSWAGELSDYDFEHFSLPYLRRVSTLVKEGLSQANIHPVPMVVFAKGANLPHQLQQLASSGYSTVSLDWKVRPAYAIEALGSLSNSIALQGNLDPAVLHTDHQTIKKYVRLMFANQEAGGFLPHLPHITNLGHGITPGVDPEAMRTFLEAVHEESTKIRQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KFI2</accession>
    <name>E3KFI2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_09010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EFP83057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327476.1">
        <property type="nucleotide sequence ID" value="XM_003327428.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KFI2"/>
    <dbReference type="GeneID" id="10544769"/>
    <dbReference type="KEGG" id="pgr:PGTG_09010"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09010"/>
    <dbReference type="HOGENOM" id="CLU_1960660_0_0_1"/>
    <dbReference type="InParanoid" id="E3KFI2"/>
    <dbReference type="OrthoDB" id="10287874at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP83057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="128" mass="13938" checksum="AC14A6DA243DBB9F" modified="2011-01-11" version="1">MFLRCWKPPWVYLGPLDTFASAIVSSINTVLASVFPRFPHQPTSPASCINSPSNTHHVHRVVFLSCTSSFTTTTMSPETSNMSSPVEIPAPCSYQAISNPTPVDDVSSGRSFPARGNWSPLLCPTLRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3KFN2</accession>
    <name>E3KFN2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HPt domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_09049</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EFP83096.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327515.2">
        <property type="nucleotide sequence ID" value="XM_003327467.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KFN2"/>
    <dbReference type="GeneID" id="10544808"/>
    <dbReference type="KEGG" id="pgr:PGTG_09049"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09049"/>
    <dbReference type="HOGENOM" id="CLU_2050787_0_0_1"/>
    <dbReference type="InParanoid" id="E3KFN2"/>
    <dbReference type="OrthoDB" id="1673781at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.160">
        <property type="entry name" value="HPT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036641">
        <property type="entry name" value="HPT_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47226">
        <property type="entry name" value="Histidine-containing phosphotransfer domain, HPT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP83096.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="120" mass="14166" checksum="47EF96C25DCC00A9" modified="2012-04-18" version="2">MADIIDTRRLWRMMQLDVNNINVQDLMVRYIGQSIQRTTTMKDMLEIRNYDRLGQEARHLETSASALGLVEIGPICEYIITQCELSPTIHRHRDIEDRIRLLEGANRRAQTWVLTYLSHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHM0</accession>
    <name>E3KHM0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_09508</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328214.1">
        <property type="nucleotide sequence ID" value="XM_003328166.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHM0"/>
    <dbReference type="STRING" id="418459.E3KHM0"/>
    <dbReference type="GeneID" id="10532482"/>
    <dbReference type="KEGG" id="pgr:PGTG_09508"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09508"/>
    <dbReference type="HOGENOM" id="CLU_006838_0_0_1"/>
    <dbReference type="InParanoid" id="E3KHM0"/>
    <dbReference type="OMA" id="CEDFYGS"/>
    <dbReference type="OrthoDB" id="2501302at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158791">
        <property type="entry name" value="MgtE N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WW" evidence="2">
        <location>
            <begin position="229"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="437"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="598"/>
            <end position="908"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="960"/>
            <end position="1263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="327"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="362"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="378"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="389"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="437"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="520"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="535"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="608"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="740"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="798"/>
            <end position="810"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="866"/>
            <end position="875"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="968"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1078"/>
            <end position="1088"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1102"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1132"/>
            <end position="1152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1198"/>
            <end position="1208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP83795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1263" mass="140171" checksum="2B8487FB346F9FA2" modified="2011-01-11" version="1">MDPEDVLDFGEDDDGLDVISLGASDHDGRPVSNRSSSTTVESTTRSVRQPRPSSPTSNRHLRTTSHTQPNGSDRRRHGSVHRAANPPQAPLRDRNHQSALHPADRSAPHPDRRLLVQEASRHAEQSSVTAPPRERPPSDRPRPGERASYMPPERLHAVSASSRTSDPTTSKTDSRRPVASLSTRITTDHRDADQKPPNPLSSSKPDAEREKFEEAKDVEKPVEPSITSTELPEGWISRISKGSKKTYYVNALTRTSQWNRPTEPASGKPTKPAPKVASKAEVNQPVQEATESRTHPSRSNLSKIDSMDSSVDSPLLTRCQQERGPNIQASQSLDNQALNLRPSSTTVSHDRHQLATPQEPSRYYDTDKSTMDDHHRQSNLIGSGTAHASDSDIDGRSIHQEKLQPDPSESCVDHRIHPDRRQEIASAIHPDRRQEIASAIHPDRRQDIASTVHPDRRQEIASAMHPDRRAEIASPKPHRGRISAPEGSKATIIGSFHRDLPPHSDSNNRNSSRHAVTTRYPHDSGSHRSAEIPRPRSISPVPRRVASRRSGRRDHREHIQLSGANGIPTAPGRCFPDPIAPLQTHEMYIDPEVSRFGHPLAIPPKMTEPPDYRDVSYRRGPEQPYDRDTSSQDRRASSWTRHEAAPISPIHPLPRHSLALRPRSPSPPPRRMPPLMHVPRAPLQPDLYPPERTRSPPTSHRLLLEERSIRVPEPSESFRRNPAPRYDAYRPSPSFPSETKGPRPLDPRRGDAYYPRDQDPHRSEPSLLDIRRSQVAQHLPLLPEPPRIRSGMSQPDLYRPPPRPVHPPRLSPRRPEPNNYYPIIDLDEISNKNDPPPGPSAVRISQVPPPATYPVKETPQPPLKQPSDRSQDLNKIKLVSDIQRKPQANSTRELPIDDSKQPLTDDAAHQLLTDDAVHTPPDYPIQTPPRNARKLLVDDASKIHISDPSKTVMDTANVPAMATLGIPKHNDRSRNGRRGRSIEPDDLSEKDANASSDIIEIPPPPLTPVDSQTSGQPVSLAKRLGPVADCSGQSDKFSPPAKRSRRNPVDLTEACDVSNDFADNSKALPPSTEEKSSSKQNLPSSSHNSSEKIAVQSPVAERPAKVDQKPETDHSVHINQDRGISLRGRAGRKSEHTGVPEQHRSQVEKKGPSDPIPAGTGSLRDRIQDLAFSPVPPVQSRVPSQQPSPGPGPQRSQATHHHEVHHGGARPNQDPPVVRHISERISGFRVPLGHSSPSHGSHRARYAPRQSDSGWSGRKARYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHR1</accession>
    <name>E3KHR1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09549</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP83836.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003328207.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHR1"/>
    <dbReference type="GeneID" id="10532532"/>
    <dbReference type="KEGG" id="pgr:PGTG_09549"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09549"/>
    <dbReference type="HOGENOM" id="CLU_2321462_0_0_1"/>
    <dbReference type="InParanoid" id="E3KHR1"/>
    <dbReference type="OrthoDB" id="2508245at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173900" evidence="1">
        <location>
            <begin position="20"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83836.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="99" mass="10899" checksum="7564C52E86D46AD7" modified="2012-04-18" version="2">MNILLYLILLGTFVHLNLATPLFWEPLDFNFSSFGRLGALIKAVSQTNECFEHVPKSNPAIPILRSKSPGGIPHVSTVTAFRDDAGAPIRKSQLPMHNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
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    <name>E3KIT1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10584</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
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    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP84206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003328577.2"/>
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    <dbReference type="AlphaFoldDB" id="E3KIT1"/>
    <dbReference type="GeneID" id="10545466"/>
    <dbReference type="KEGG" id="pgr:PGTG_10584"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10584"/>
    <dbReference type="HOGENOM" id="CLU_2074301_0_0_1"/>
    <dbReference type="InParanoid" id="E3KIT1"/>
    <dbReference type="OrthoDB" id="10358436at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="63"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="118" mass="12677" checksum="39E2211D55C4E3F9" modified="2011-01-11" version="1">MLSWITSLATSSPDSSALHNHHRPVFNPYLVQISLIMHIKSVTAICCLASYALGMPMESKELAAGSKLGSESKGAKTNKPPDAERCHNCGCVLTEEDTAPPPKEKIKGSKSTKSKPKK</sequence>
</entry>
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    <accession>E3KJP4</accession>
    <name>E3KJP4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_10239</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
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    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178290">
        <property type="protein sequence ID" value="EFP84519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328938.1">
        <property type="nucleotide sequence ID" value="XM_003328890.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJP4"/>
    <dbReference type="GeneID" id="10546109"/>
    <dbReference type="KEGG" id="pgr:PGTG_10239"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10239"/>
    <dbReference type="HOGENOM" id="CLU_051119_2_0_1"/>
    <dbReference type="InParanoid" id="E3KJP4"/>
    <dbReference type="OrthoDB" id="2498930at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027532">
        <property type="entry name" value="Mdm12"/>
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    <dbReference type="PANTHER" id="PTHR28204">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204:SF1">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP84519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="255" mass="29288" checksum="BA0D1FF15075540C" modified="2011-01-11" version="1">MATEIVRESKILLNNDNYALWLLPMKAKLHKAKSLTIVTGIHTYAYAEIVQHLNQEVLAYVSSTQTETDEFNGYKLWQLLKQKYAGNDLTSRTTALKKYLAVEYDSFSAFLPLIRSANQKIVLSKLALDDQVKTILMLDKLPPEFHSFKTNISMNFETESFEEVLKKLEDFAAQNQLNELKKRSTSPISSQATMYTRSSDPDISGHTEDSCYQKHPEKRAPKSVASSSKQHSSHLTRYTQEDKEYLQQKYPDLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJU5</accession>
    <name>E3KJU5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Superoxide dismutase copper/zinc binding domain-containing protein</fullName>
        </recommendedName>
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    <gene>
        <name evidence="3" type="ORF">PGTG_10729</name>
    </gene>
    <organism evidence="3 4">
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        <lineage>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003328941.2"/>
    </dbReference>
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    <dbReference type="GeneID" id="10546159"/>
    <dbReference type="KEGG" id="pgr:PGTG_10729"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10729"/>
    <dbReference type="eggNOG" id="ENOG502QUBE">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_719892_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJU5"/>
    <dbReference type="OMA" id="NGDACIM"/>
    <dbReference type="OrthoDB" id="3241054at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021476">
        <property type="entry name" value="Egh16-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34618:SF1">
        <property type="entry name" value="SECRETED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34618">
        <property type="entry name" value="SURFACE PROTEIN MAS1, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11327">
        <property type="entry name" value="Egh16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173647" description="Superoxide dismutase copper/zinc binding domain-containing protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="300"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="332"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="373"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP84570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="389" mass="40662" checksum="8C9A71390FFA2A6E" modified="2011-01-11" version="1">MVKPLSILPVFSVFLPQVLSHSFIIALDGANGVQSSGFGTRLTTRGQVHQYTGIITDKEIKAGTVGPCGKIFGGDNFPPFVIDPHAELARAEANGVSTVHKDGSIVMGVFVHNPDGSGPFKCDYSPDASLSKFEPMNITVQIEGVDGVNPAAENYVYPLTAAFFPNATCTGGKGKDTCIVRCRNQLGFGSCAALKLGEAAKRAKPSHPSNSTHANHTAEGEKEGHKTGANNTAAKNQSNSAPLPPPHEPKPVNQTNTPPVADPPAPINTTVAPPPPAASPPITAAVVQPEPPQPAVNQPGAPQSSQLTDGPNPDAFRIYNSGAPPPEPPAVAPASPGNNPNVMSSSPPPHDPNYNALTADIPSSPAGVERRSLPKTKRLFLHKRPRRHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJZ4</accession>
    <name>E3KJZ4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Elongation factor EF-3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10778</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84619.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329038.2">
        <property type="nucleotide sequence ID" value="XM_003328990.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJZ4"/>
    <dbReference type="STRING" id="418459.E3KJZ4"/>
    <dbReference type="GeneID" id="10546288"/>
    <dbReference type="KEGG" id="pgr:PGTG_10778"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10778"/>
    <dbReference type="HOGENOM" id="CLU_1918108_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJZ4"/>
    <dbReference type="OrthoDB" id="2110130at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050611">
        <property type="entry name" value="ABCF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19211:SF5">
        <property type="entry name" value="ELONGATION FACTOR 3A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84619.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="132" mass="14901" checksum="D7D08FDC6704129C" modified="2012-04-18" version="2">MDYLKSLETCTYILASHSSGFFNTMRTLSGGQNVRVILAAATWRRPHDVVILDEPTDHSDRESLLALIESLKTFEGGVLVITHNREFSGSICSEFWAMIDGYQEASGHNWTEGQGRRRRALECFGEQDRSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3KK18</accession>
    <name>E3KK18_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10802</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84643.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329062.2">
        <property type="nucleotide sequence ID" value="XM_003329014.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KK18"/>
    <dbReference type="STRING" id="418459.E3KK18"/>
    <dbReference type="GeneID" id="10546312"/>
    <dbReference type="KEGG" id="pgr:PGTG_10802"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10802"/>
    <dbReference type="eggNOG" id="KOG1703">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_464711_0_0_1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
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            <begin position="87"/>
            <end position="103"/>
        </location>
    </feature>
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            <begin position="113"/>
            <end position="123"/>
        </location>
    </feature>
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        <location>
            <begin position="124"/>
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        </location>
    </feature>
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        </location>
    </feature>
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            <begin position="195"/>
            <end position="207"/>
        </location>
    </feature>
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            <begin position="208"/>
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        </location>
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        </location>
    </feature>
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        </location>
    </feature>
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            <begin position="269"/>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="316"/>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="561"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84643.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="587" mass="63114" checksum="249FB46D879E78BE" modified="2012-04-18" version="2">MALAFFSAPALPGLWGFGKAAEASDDSKPRPESMSAEAVIKCSDCGGDVVLAQLGEHICTPSDGQLATIPEDEQAEPDSENILAKPPELDKTSKPDLTEKPSKEAPVTPAPHTRNSTASIGKATSSSGLSTTSSSSLSTTSSSSLSTDSTSKSTAQTSSQSSTSTSSLDSLKEKLLNSMRPDLKSDQSSSTSPSKINMSSQKFKATASSEDSFSNSETSSSSNSSSGRMPFFERYAQLMPKTTNNPSSKATSRDAEREKDDVRFPAKKPLSPITSPESSPSPFQTSYQSSQLPQPKLPTSKSMPFSNLTALGAASQRREALTRNTTKEERKMPNSSSAASGLDRLIAREHEELSRTVQHTRNPRPLKDTDHHPRAQVPTTTSSKSRTRTNDPSVTGLEDLMGDLMAEMEKKAELSSEEYQKRNRQPVVKKILVPETTTPKSSSPGRFKHRDYETDTPRKYPSAYPDDTPRIHGRQPSDPSSESSPSSPGIRPSDSISQLGYTRSPSPRLAKPLAVLTGDGGYSGVRTMHGDADPTRQEPSDGRGPVLRGMLRRSVPVQVQEMREADRAGRSDRSGREGLGQVSCGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3KK72</accession>
    <name>E3KK72_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Translation machinery-associated protein 22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_10856</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2">Interacts with the 40S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DENR family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84697.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329116.2">
        <property type="nucleotide sequence ID" value="XM_003329068.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KK72"/>
    <dbReference type="FunCoup" id="E3KK72">
        <property type="interactions" value="674"/>
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    <dbReference type="STRING" id="418459.E3KK72"/>
    <dbReference type="GeneID" id="10526664"/>
    <dbReference type="KEGG" id="pgr:PGTG_10856"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10856"/>
    <dbReference type="HOGENOM" id="CLU_073511_0_1_1"/>
    <dbReference type="InParanoid" id="E3KK72"/>
    <dbReference type="OrthoDB" id="277199at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11607">
        <property type="entry name" value="DENR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050318">
        <property type="entry name" value="DENR/SUI1_TIF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046447">
        <property type="entry name" value="DENR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789:SF0">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUI1" evidence="5">
        <location>
            <begin position="39"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP84697.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="139" mass="15413" checksum="CADD9ED6BE2C89B1" modified="2012-04-18" version="2">MSVEEVEKKKKAETEALKKEAKEDAKASRIKEKSKTNKVTVKTVKRTKRKRITTIHGLDLFGVDLKKLAKLFASKFATGSSVTKNNQGEDEIVIQGDVSDEVLDLFDSTTGKFAEIIGNGIIPDDDIVFVDDTKKKKPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>E3KM67</accession>
    <name>E3KM67_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fork-head domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_11384</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178294">
        <property type="protein sequence ID" value="EFP85215.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329634.2">
        <property type="nucleotide sequence ID" value="XM_003329586.2"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KM67"/>
    <dbReference type="GeneID" id="10527203"/>
    <dbReference type="KEGG" id="pgr:PGTG_11384"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11384"/>
    <dbReference type="eggNOG" id="KOG2294">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006224_0_0_1"/>
    <dbReference type="InParanoid" id="E3KM67"/>
    <dbReference type="OrthoDB" id="5954824at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00059">
        <property type="entry name" value="FH_FOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000135">
        <property type="entry name" value="forkhead box protein G1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001766">
        <property type="entry name" value="Fork_head_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045912">
        <property type="entry name" value="FOXJ2/3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46078:SF2">
        <property type="entry name" value="FORK-HEAD DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46078">
        <property type="entry name" value="FORKHEAD BOX PROTEIN J2 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00250">
        <property type="entry name" value="Forkhead"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00053">
        <property type="entry name" value="FORKHEAD"/>
    </dbReference>
    <dbReference type="SMART" id="SM00339">
        <property type="entry name" value="FH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50039">
        <property type="entry name" value="FORK_HEAD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Fork-head" evidence="7">
        <location>
            <begin position="884"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Fork-head" evidence="5">
        <location>
            <begin position="884"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="94"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="183"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="276"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="368"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="434"/>
            <end position="702"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="721"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="772"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="836"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="968"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1034"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1101"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1144"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1182"/>
            <end position="1209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1231"/>
            <end position="1413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="95"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="106"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="230"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="285"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="310"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="436"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="476"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="493"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="520"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="538"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="617"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="649"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="721"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="978"/>
            <end position="991"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1062"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1101"/>
            <end position="1110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1111"/>
            <end position="1123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1306"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1339"/>
            <end position="1370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP85215.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1413" mass="150719" checksum="5DAA9CBCA48AB4DD" modified="2012-04-18" version="2">MTSTNRAKQIIPEKLRASIQLGVLFTTSLRAERRLFTARTSFLPSSINLKPTLPNSRIPSSTQTILILRRKEIIKLFVVVCSAYDRPTNYYCSLSKKSSSQPPSQTLRSPPLSTRPSKSNAIIETLSSKLTTSSFATLLPLTASSRLPLSSSSALALRSTTNTPPPTNSELCKSWIQFTNALTQQSSSHHHPSATIPHLTTPQPRAPPAPVNRPKSASLGQPNLSPPSPGDLSPSSTDSSADNTPLSPPLRLLSVADLHLNSPAISSQSFPQLTGPHLDHILPLNNNNNSSSNNNNNNNINNNNNSSSNLVHSSPQTNHRAITNHHRHPSLSRSNLPSPSLTSGYGFGELAGALGVRPEIRKVSDMTIHSADHPPSPVDPINLDQTRHHHPPPPPQSEPLRSDALLGHDHAEIVHQSHHQSTAVGVDSRLERAPNTLSDFQPPSSSSSSNLTTTAESTTEQSQQDTINETHTNLYASSPIITPSIPTTTAPARSNQTPLHSDTTNLHHSSSTTYPRLPLQYSTSSQSHASFQSQQGQINTSLGPNQSAYQSDCISSFEHTPHSTVGLNNPTSGYHPSPFIAHHRRSHSKPDGRSSMFVNPFLGAPSSHTNPTPPSPGASSSHSHSFYHSHAQPRCSLTAAPTPTRHRLSISSQPGGASSSPSLLSGANRHRSSSTATGTARGCDPVHRSAMGLASPDVGSSPSFGTAATAMGLLSHITSPNNFTKDSQSSSHSFEALGRKPDSRGSANQLEHSNQMEHTSHMEHLIKQTGQIEQTAGHMEHTTSHMEQPGQLEPTSQTDSSDQKPLHSLHPHSPLTPGFAGSASFSFGNSASNRVKCEIPPNSDMADLSTGPVGSSNSAMNPIPINMRPDLANLKDGPDGPNGKPGYPYVVLIRYAILGSPNGKLTLQELYETIMDRFPYYRTAGKGWMNSIRHNLSLNRCFVKQPRHILDPGKGSYWTVDLEAELSTSRARDRKRASGSSRSSIGSIRSLKGSRRDSLSGDPRSPTLDEIIGSSPSRMLYMSDEETDEVHVQPLGSFAPEHPTTFAEDGDGDTMMQGGEGSFQQHPSTPIKSPKSGRISAKRAPKLTKMKQQGLKVQVSGNSDLTSNHLPPSSCSPSTPVTPHQLYHQADPSSNILQSPFHPQAGYVSSPPDTIGRTIPRTGSPYRGSTLMRSPLTSIMSYSSSSHCPTTATTSDSNQIDPISPSSSAYGLNTGLSSNYHPSSTAMVNCREGGPPSQNPFSFGAHPHSAYQPGFNPFSPPLMHHPQSSPSPAMGDRHPASAGPWPSSFQASAYPTSAPIRGVGTSHHQPSQSHSGVYGFQPSSMAGIVPRPRFSVSAGGNSRPVSSESSSTNNPPHSTTGRQTPASNDSDASRRSSIGGGPGGSTNFVRIDGFGNGNPAVDSLGRTHSSQPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3KMN2</accession>
    <name>E3KMN2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11913</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178295">
        <property type="protein sequence ID" value="EFP85557.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329976.2">
        <property type="nucleotide sequence ID" value="XM_003329928.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KMN2"/>
    <dbReference type="STRING" id="418459.E3KMN2"/>
    <dbReference type="GeneID" id="10544117"/>
    <dbReference type="KEGG" id="pgr:PGTG_11913"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11913"/>
    <dbReference type="HOGENOM" id="CLU_008138_2_0_1"/>
    <dbReference type="InParanoid" id="E3KMN2"/>
    <dbReference type="OrthoDB" id="107110at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266">
        <property type="entry name" value="CHROMOSOME 15, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266:SF1">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173536" evidence="1">
        <location>
            <begin position="25"/>
            <end position="876"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85557.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="876" mass="99783" checksum="1C4C7A4F4D3A8781" modified="2012-04-18" version="2">MSKSSLHLLRFCLSYFCEIVGVLSSFHISRFQGLTDHTLLKILPIVSMNSSSPERKGVASDEDAETQLCLQYAGLHSNERYPDDQSFLRLLQESSASRVKEYLDELTEAYSRAEDQPTAFRNEIWHAFLLRKPRAWSTMRSIYKLEEDVDLDKEFIELAFFHQYPRWSQQGNVSYREMLDALLEGSVAAGEELLAAIEPESKHTQILKAGYRHPYLRHETILKPILKRLSEATQKWTRGEYLAPYTTLLGPNLVGKTRLLQQLSKNICVIYICLCPHNSTGLPSRSPLADEMVPAAKNFYELELVYTRFLAAVFHVVAEFFSKQPAQISEEKRLKKWFKFNSEYEDEFASRVRERMREVSNAKLDDHATLGTSLKKMHESTCFIQNPHLKVLLAIDEARELAKLETRDFLRESYFLILRQVLSTIPGSLGFFAIFTDIPLAAAEPNRTIFYDPTGRPDPGSIPKELFPPIYDIGTFDSKVPPGRPKTWAELLSPRRLFSYGLPFFRIYVEGAEEKGMTDHMIVRNIGQIATQKLLGFTKAHEPLSEGQVFALMGSTIQPRTTSATKLNSELISNHLAMCLYISPSRDRVISEYPSQFACSMAANHFLATDESRLIDCIDALTSALQPGVISSGNAEELASRIILLRAMHKAMSISEDQIDIPYGCSVRLVDFLCALTGSEEQDLELGDIESQHKKRLLTEARIFWNHFIQITYTPHASDLLEFMYRGMAVQCMPMQPGFDQLFTIYFDSGDPNSVLTKDHISFCGVRVKNEEFSFEREASEWTDEYAEIKINPENPYLSILFSFKTQSTQQQSLPKILNRGSLIFHGLTDIKCLTAGISEALDKLLAVEDDVRGFHKENQMKNFLETASPVVYPRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>E3KMS5</accession>
    <name>E3KMS5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_11956</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178295">
        <property type="protein sequence ID" value="EFP85600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330019.1">
        <property type="nucleotide sequence ID" value="XM_003329971.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KMS5"/>
    <dbReference type="GeneID" id="10544160"/>
    <dbReference type="KEGG" id="pgr:PGTG_11956"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11956"/>
    <dbReference type="HOGENOM" id="CLU_2062638_0_0_1"/>
    <dbReference type="InParanoid" id="E3KMS5"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP85600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="119" mass="12714" checksum="7D5344EB34D21054" modified="2011-01-11" version="1">MICYWLKIEERAPHEAKALATTTAKVQVVVTQLLAPQSLPSGSDQLSIPRSLSSDGLKLALVCEVVESILVHIHGIASAMWKRGGLNGCIQAVRSSQSASSFTRKPSSILGSKAINFLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNM8</accession>
    <name>E3KNM8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_11659</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178297">
        <property type="protein sequence ID" value="EFP85903.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330322.2">
        <property type="nucleotide sequence ID" value="XM_003330274.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNM8"/>
    <dbReference type="GeneID" id="10544485"/>
    <dbReference type="KEGG" id="pgr:PGTG_11659"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11659"/>
    <dbReference type="HOGENOM" id="CLU_157430_0_0_1"/>
    <dbReference type="InParanoid" id="E3KNM8"/>
    <dbReference type="OrthoDB" id="7763131at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="91"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP85903.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="133" mass="14354" checksum="ECEEB6EDC6149497" modified="2012-04-18" version="2">MATAQVEIAAQSKRQNDIYLSQIETMKTMANAAIMNKDISGLDDVTQEFYKLQREQIMLQLRERSKANAVEQAKQAEVERARSATSATILTQGSTCSQASGSTQTQSTTSSISQAGTRTPTQATTSTTSIDIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNP2</accession>
    <name>E3KNP2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Prolyl 4-hydroxylase alpha subunit Fe(2+) 2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11673</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178297">
        <property type="protein sequence ID" value="EFP85917.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330336.2">
        <property type="nucleotide sequence ID" value="XM_003330288.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNP2"/>
    <dbReference type="GeneID" id="10539916"/>
    <dbReference type="KEGG" id="pgr:PGTG_11673"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11673"/>
    <dbReference type="eggNOG" id="ENOG502QWAB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007520_1_0_1"/>
    <dbReference type="InParanoid" id="E3KNP2"/>
    <dbReference type="OrthoDB" id="124582at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.620">
        <property type="entry name" value="q2cbj1_9rhob like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044862">
        <property type="entry name" value="Pro_4_hyd_alph_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33099">
        <property type="entry name" value="FE2OG DIOXYGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33099:SF7">
        <property type="entry name" value="MYND-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13640">
        <property type="entry name" value="2OG-FeII_Oxy_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prolyl 4-hydroxylase alpha subunit Fe(2+) 2OG dioxygenase" evidence="1">
        <location>
            <begin position="130"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85917.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="849" mass="95865" checksum="082CB1866571722F" modified="2012-04-18" version="2">MARYSNYRSPDKFKKGLYEAFQAITTPGTYAAWEELATTPPAGLHVDGVGDIAMPLAEKRVRELIANAHQAPYGRRSETLVDLSVRNTWEIDGARLRFLDPAWKRYLKSLAKRVAVLLGVDGRIRADLYKLLIYEKGAMFKSHTDTERTPGMFGTLVICLPSAHAGGEVVVKHNGQTKTLRTSDATQSFACWYSDVTHEVLPVKSGYRCVLTYNLVTQPDRPRPKASVVESQKDPLRKMLGSWLEDLAANSSTPSYLYHTLEHSYTEASMSLEALKAQDFARVQALQDLANELPFQIYLALVERMEQGDTEVPFPRKRHRCVSKFESVETEDEDVDCPFFQVDLEWDRQVKSLHKLDGIIVARDYHLNVNNFVEEDPFRCVDGTEDNYKPYQGNWGPSGSPAIHWFRRSAFVILPRQSLGNYLAECCGRDQYSTPLNMPVTFFDWAEEWLNTLPEAERAEKYRTWIPSIIHRHQSVADRIDFVKRISNFINNSALPARSFPDKSWADGVVHDIIKNLLKVTTKGSAVPNAADGNAIVSAIFQLDETWPAKLELITTLFEAFPQSQAVGFLLGLLSQLKTLGTAPQLPIPDTTELRRRLSLRLFNNERTPSTIITHSSKRGGEKQIVVTPQAMVEFACDLHDLSTDTNNLLELFILQIKDHCPKFPGEGIRKAWIPFLCQLIPALVSRSISINTPPYQQLARKLVKYGVKKLGPCPQPDPNTPRPRIRCPCSDCVSLKRFLRDPNQVVGRFQLPQARRNHIYESLDEPGFDCIRKTEHIGRPHTLIVTKRLTLENKIKDWKDLRFEIYGPLAKNIQPELLEALLGVQGAAVVQSLGGIQPAPAVSTTRAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNU9</accession>
    <name>E3KNU9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Myotubularin phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_11730</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178297">
        <property type="protein sequence ID" value="EFP85974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330393.1">
        <property type="nucleotide sequence ID" value="XM_003330345.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNU9"/>
    <dbReference type="FunCoup" id="E3KNU9">
        <property type="interactions" value="178"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KNU9"/>
    <dbReference type="GeneID" id="10539973"/>
    <dbReference type="KEGG" id="pgr:PGTG_11730"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11730"/>
    <dbReference type="eggNOG" id="KOG1089">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001839_0_1_1"/>
    <dbReference type="InParanoid" id="E3KNU9"/>
    <dbReference type="OMA" id="RTMEGFM"/>
    <dbReference type="OrthoDB" id="271628at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004438">
        <property type="term" value="F:phosphatidylinositol-3-phosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030564">
        <property type="entry name" value="Myotubularin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010569">
        <property type="entry name" value="Myotubularin-like_Pase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016130">
        <property type="entry name" value="Tyr_Pase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807">
        <property type="entry name" value="MYOTUBULARIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10807:SF128">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-3,5-BISPHOSPHATE 3-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06602">
        <property type="entry name" value="Myotub-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51339">
        <property type="entry name" value="PPASE_MYOTUBULARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00383">
        <property type="entry name" value="TYR_PHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myotubularin phosphatase" evidence="5">
        <location>
            <begin position="177"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="18"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="56"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="650"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="689"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="787"/>
            <end position="958"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="56"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="161"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="703"/>
            <end position="733"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="734"/>
            <end position="758"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="804"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="815"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="911"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphocysteine intermediate" evidence="2">
        <location>
            <position position="418"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="329"/>
            <end position="332"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="354"/>
            <end position="355"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="418"/>
            <end position="424"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR630564-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP85974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="958" mass="105568" checksum="A37B03C6035889EA" modified="2011-01-11" version="1">MEVIKVPKVEHVRLAKLSHQFGSPSPSSSATQDSNDPPGLTGSLLITPHHLIWSHSLNSGPDETPSNQLNAESSKSCGTSPGSPGKAEDVWIAHSLLTAVEHLPRHREPTLLIRLATFVFYTLSFSSPNELDDVWDSLKALTSNRKPGEKEQLFAFVQGATSKSNPSSTDPSSTLPGWDSYDPHAEFTRMKVIGESSKKSENNPWRLTTINKNFNFCSTYPSVLLTPSKISDTTLTYAVKYRSKGRLPIGTYVHWANGSSITRSSQPMVGFKNARSIQDEKLIEAIFHSHSLHTGPVAVGDQPSQQVVYGATSSNLIIDARPTTNAMANTVKGAGTENMEYYKGCRKAYLGIDNIHVMRDSLGKVVSAIHESFVTGTPVSLDSLKRSGWLKHIGAILEGTSLITRTVHVYNSHVLIHCSDGWDRTSQLSALSQVCLDPYYRTFQGFAVLVEKDWLSFGHKFSDRSGIVISGRERVSFGDSPNHSNYSSNIARDDPDESISGSGAWVTSIQKQLNFGHSVGYSHAYKETSPVFHQFLDCVYQLQCQYPKRFEFNSCYLERLHEELHAGNYGTFLFDCEKERIEAKASSLTRSVWELFLPPNDISKYKNPQYDPSLDDPSNRLNTDQGVLYPEVKDVIFWWEIFGCPNEEMNPPKVPDPSPNLDFTHVEPVSSQTDEITECSETLENLTFETVASPPPGPLRASNGPQDPSTPKTLPSNLKTRSFVSSSSINDAATSTTSSQLSSSYSETQFSTSPNPAADQFQNAFQQAKIMGWSTWDRLKKGYEEVARGSGPATSPRVYHHQSPSRSPAPSTSTNNRRSLSPQHQQYPLNPDLNPWSAPDPRPSSSPLNSSPTPARPASHEHSHGALSLPINDPWQTSSEDEPRSILSTLEPRAHSPRHLPPISHDDIDTLLSSTNPAKDPNTQKAPLSFKSGSSLSQQETSCRAQQADIDPLGVGFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3KP19</accession>
    <name>E3KP19_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DUF726-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_12000</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178298">
        <property type="protein sequence ID" value="EFP86044.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330463.2">
        <property type="nucleotide sequence ID" value="XM_003330415.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KP19"/>
    <dbReference type="FunCoup" id="E3KP19">
        <property type="interactions" value="8"/>
    </dbReference>
    <dbReference type="GeneID" id="10540045"/>
    <dbReference type="KEGG" id="pgr:PGTG_12000"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12000"/>
    <dbReference type="eggNOG" id="KOG2385">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028425_0_0_1"/>
    <dbReference type="InParanoid" id="E3KP19"/>
    <dbReference type="OrthoDB" id="277931at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007941">
        <property type="entry name" value="DUF726"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17920:SF23">
        <property type="entry name" value="DUF726-DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17920">
        <property type="entry name" value="TRANSMEMBRANE AND COILED-COIL DOMAIN-CONTAINING PROTEIN 4 TMCO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05277">
        <property type="entry name" value="DUF726"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="317"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="353"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="43"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="99"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP86044.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="668" mass="72812" checksum="119393E0297B7A8C" modified="2012-04-18" version="2">MDNPTKVDLFYERWEPFHLQIVSIAVKYASEEVQANRLEESFQPLCSPSLPPTTPPTTTPTPLLVDSLPPPEPNTTAHSSDNHYPTLKELSSIDSTIEKEEKVDHPKTTTTIGYSRVPTRSEEEKIKEEEIAAEKAEELDHGVHQEWLETRKKGCEGWMKFILESVKVDPASLPEKPLRTDLTELSKEIPSTMHVEIIHDLVIFSLSASHISPTNAPLSSSKPTTNENLNYSALDRQLVFQAAEALGIEPTIVYGAEKLVAQELFFILQQQNEAASKQQGDGEVEDDQAETTPESKLTKSSKAAILAAAGKKKWLRYLGAGAGVIVGGVAIGLTGGLAAPVLAPFLVGLSGGALGFLATSGGAILIGTLFGLAGGGLVGYRAQRRLKGIDEFTFERLPDQDGEVDGDLPRIPSLHATIVSSGFLLTATEYKDMWMPTLSKTIDRRDVYALKVETGALLSAGKDLETYIRDTLLQHGATEIIRHTVLASVCAALLLPASIYKAAVMALDNEYQRTRDICEKAGILLADMIEQKAHGARPLTLIGSGMGSITIVRALLELSKRGGGAAEFQIDQVILICSPLSPDPIEWKTIRRMTSRRVVNVYSKNDWVLAILARLDSLLSARRVAHVAGLRDLNLPHISSVDVSDLVHGHLDLNSKIPLILDRIDIHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQF6</accession>
    <name>E3KQF6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_12487</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178300">
        <property type="protein sequence ID" value="EFP86531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330950.1">
        <property type="nucleotide sequence ID" value="XM_003330902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQF6"/>
    <dbReference type="STRING" id="418459.E3KQF6"/>
    <dbReference type="GeneID" id="10540548"/>
    <dbReference type="KEGG" id="pgr:PGTG_12487"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12487"/>
    <dbReference type="HOGENOM" id="CLU_055324_2_0_1"/>
    <dbReference type="InParanoid" id="E3KQF6"/>
    <dbReference type="OMA" id="TEDILLC"/>
    <dbReference type="OrthoDB" id="76487at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023:SF4">
        <property type="entry name" value="GLYCINE-RICH PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="3">
        <location>
            <begin position="130"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="148"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="239"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="177"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP86531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="312" mass="35594" checksum="4EC5EE22AB60EFBC" modified="2011-01-11" version="1">MATPSTPVEETTDASQPQTKQKRGPNWLPREEEQLAISWINISEQPEFATNQSGKTFYRKIEKDFNTHSKANYRNHAQIKTRWTAMNTATLKFSAIYNAIERNPPSGSSPDDWLEAAKMNYLDQTKGTAFSNLPAWQKLRYAPKWRADPRVDQPATPLPTTLSDSIDPESAHDENPGMSSPTVRSASSISRPIGGKAAKKRRIEGYQHEELLSQTSDLAVISQERLTAFHKGNEILINKNNIMKEKIEIERKKLQLEQEKLAVEKEYRQSETDMNDFKILRDAEDLGDEEAKDVIKIIKDQIKNKWRARAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQV9</accession>
    <name>E3KQV9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_13066</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178301">
        <property type="protein sequence ID" value="EFP86684.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331103.2">
        <property type="nucleotide sequence ID" value="XM_003331055.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQV9"/>
    <dbReference type="FunCoup" id="E3KQV9">
        <property type="interactions" value="110"/>
    </dbReference>
    <dbReference type="GeneID" id="10540946"/>
    <dbReference type="KEGG" id="pgr:PGTG_13066"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13066"/>
    <dbReference type="HOGENOM" id="CLU_906535_0_0_1"/>
    <dbReference type="InParanoid" id="E3KQV9"/>
    <dbReference type="OrthoDB" id="1747252at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_uL1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL1M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="225"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="296"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP86684.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="343" mass="37579" checksum="B1C6011CA60F8C44" modified="2012-04-18" version="2">MLPINQNAFNLFRSSSRTTRPRTCCKLLIFSRGAKMWVPVPQKAKTEPDNLAPLLDSLRLVKAVKIDQPDLPIELTIEPLMKKTVNANVLKGRFVLTNPVPSPASSSSGQNNESGKDVIAVILDDQSADDVRLAKELGVDHFGGQDLLDRILAEEVKPTKLLVHSKSPKIQNIFTSSESKKTLFKTLSQLNLIIPSEKRGTMSENLNELIMNSKNSIDWLVKKKPTTEKPIQPSDPTSSSPVKAAKVNNKNSKNQIDSFISIPVGSIQMNLIHLEQNISDFLSMVSNLTQFGTASTTTASKSSSNSDTLPKLKSNFKSGRPIKKPGIYKATLTVKGIPPITVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KR04</accession>
    <name>E3KR04_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_13111</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178302">
        <property type="protein sequence ID" value="EFP86729.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331148.2">
        <property type="nucleotide sequence ID" value="XM_003331100.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KR04"/>
    <dbReference type="STRING" id="418459.E3KR04"/>
    <dbReference type="GeneID" id="10541153"/>
    <dbReference type="KEGG" id="pgr:PGTG_13111"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13111"/>
    <dbReference type="HOGENOM" id="CLU_2278824_0_0_1"/>
    <dbReference type="InParanoid" id="E3KR04"/>
    <dbReference type="OrthoDB" id="2501332at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP86729.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="102" mass="11185" checksum="C6E41EF17314D687" modified="2012-04-18" version="2">MKAQMSALLINEKYQASLGQKTYDYRELIGLIDRPNALYVPDNVYDDRKRMASKRTSLGVQIGGWSSSNQIVLSGSRAGVLAACGYLNELEIANRAADLPCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3KRS1</accession>
    <name>E3KRS1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DUF159 domain protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_12737</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SOS response-associated peptidase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178303">
        <property type="protein sequence ID" value="EFP86996.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331415.2">
        <property type="nucleotide sequence ID" value="XM_003331367.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KRS1"/>
    <dbReference type="STRING" id="418459.E3KRS1"/>
    <dbReference type="GeneID" id="10541910"/>
    <dbReference type="KEGG" id="pgr:PGTG_12737"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12737"/>
    <dbReference type="eggNOG" id="KOG2618">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035990_0_0_1"/>
    <dbReference type="InParanoid" id="E3KRS1"/>
    <dbReference type="OrthoDB" id="2111841at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106300">
        <property type="term" value="P:protein-DNA covalent cross-linking repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1680.10">
        <property type="entry name" value="SOS response associated peptidase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003738">
        <property type="entry name" value="SRAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036590">
        <property type="entry name" value="SRAP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604:SF0">
        <property type="entry name" value="ABASIC SITE PROCESSING PROTEIN HMCES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13604">
        <property type="entry name" value="DC12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02586">
        <property type="entry name" value="SRAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143081">
        <property type="entry name" value="BB1717-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0190">Covalent protein-DNA linkage</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="178"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="184"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="223"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP86996.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="270" mass="29660" checksum="2CE75519027327CB" modified="2012-04-18" version="2">MPPTRYFQPTHGSLLNTINARDDKVVEPRGLWNTVKGKKRCIVLCEGFFEWLNKGKDKIPHFTKRTGGELMCLAGLWDSVTYKGTTEELHTFTIITTSSNNYLSFLHDRMPVILSDRDSIETWLDTSSGEWSSSLSKLLKPFSLDDGLVSYPVPKEVGKVGNQSADFLKRKGNIMSFFNKQTRPSGSHSSKQAASSSGPDSKQMEKKGLVGTAPAVLPGLEIEGPHDADTIKDEPKGSPAPSPKKTPIKRSAASVESDALPPEKKVRKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KTS2</accession>
    <name>E3KTS2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_13429</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178308">
        <property type="protein sequence ID" value="EFP87643.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332062.2">
        <property type="nucleotide sequence ID" value="XM_003332014.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KTS2"/>
    <dbReference type="GeneID" id="10538875"/>
    <dbReference type="KEGG" id="pgr:PGTG_13429"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13429"/>
    <dbReference type="HOGENOM" id="CLU_156039_1_0_1"/>
    <dbReference type="InParanoid" id="E3KTS2"/>
    <dbReference type="OrthoDB" id="2508053at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP87643.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="91" mass="9754" checksum="53C9409497694978" modified="2012-04-18" version="2">MAHAYAPLISSPLANSGPRPTTGIVFGAAACTPKTCIFINLAEARNSPHYTSSASHQYRFTPNGIALFAVNGTNFHIKPIAEDDEDDDETF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVH1</accession>
    <name>E3KVH1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cytoplasmic tRNA 2-thiolation protein 1</fullName>
            <ecNumber evidence="2">2.7.7.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">Cytoplasmic tRNA adenylyltransferase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="primary">NCS6</name>
        <name evidence="2" type="synonym">CTU1</name>
        <name evidence="6" type="ORF">PGTG_14395</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TtcA family. CTU1/NCS6/ATPBD3 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="2"/>
    <dbReference type="EMBL" id="DS178313">
        <property type="protein sequence ID" value="EFP88311.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332730.2">
        <property type="nucleotide sequence ID" value="XM_003332682.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KVH1"/>
    <dbReference type="FunCoup" id="E3KVH1">
        <property type="interactions" value="176"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KVH1"/>
    <dbReference type="GeneID" id="10535703"/>
    <dbReference type="KEGG" id="pgr:PGTG_14395"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14395"/>
    <dbReference type="eggNOG" id="KOG2840">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026481_1_2_1"/>
    <dbReference type="InParanoid" id="E3KVH1"/>
    <dbReference type="OrthoDB" id="198857at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002144">
        <property type="term" value="C:cytosolic tRNA wobble base thiouridylase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103016">
        <property type="term" value="F:tRNA-uridine 2-sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032447">
        <property type="term" value="P:protein urmylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01713">
        <property type="entry name" value="CTU1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03053">
        <property type="entry name" value="CTU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056369">
        <property type="entry name" value="CTU1-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000541">
        <property type="entry name" value="Ncs6/Tuc1/Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00269">
        <property type="entry name" value="TIGR00269 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807">
        <property type="entry name" value="ATPASES OF THE PP SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807:SF12">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="2" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal" evidence="5">
        <location>
            <begin position="92"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="68"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="96"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR004976-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP88311.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="372" mass="41374" checksum="B200792F0CD8209E" modified="2012-04-18" version="2">MAPCVLCSTLSAENRPKVPRRAILKRPKTGQAICRECFYLIFETEIHHTILGLGNQEKRRAEEKRTSLESTQQDQELSNTENGRMMFKKGEKVAIAASGGKDSTVLAHVMTTLNERYNYGLDLYLLSIDEGITGYRDDSLETVKRNSSQYSLPLKVLSYNELYQGWTMDKVVENVGRKSNCTFCGVFRRQALDKGADLLKVDHIVTGHNADDVAETVLMNVLRGDIARLERCTEITTGGTAKGTSGIPVKRSKPFKYAYEKEIVMYAYFKKLDYFSTECTYSPEAYRGHARALVKDLERLRPSAILDIIYSGESMASAVKSNVKRPTQQSCLRCGSLASQLLCQACALIDGLNASNNKLGSSEAKVHTCGAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3KW53</accession>
    <name>E3KW53_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ORC1/DEAH AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_14106</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP88528.1"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0016887">
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        <property type="project" value="InterPro"/>
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        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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    </dbReference>
    <dbReference type="InterPro" id="IPR049945">
        <property type="entry name" value="AAA_22"/>
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        <property type="entry name" value="AAA_ATPase_RarA/MGS1/WRNIP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13779:SF7">
        <property type="entry name" value="ATPASE WRNIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13779">
        <property type="entry name" value="WERNER HELICASE-INTERACTING PROTEIN 1 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13401">
        <property type="entry name" value="AAA_22"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="176"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP88528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="295" mass="32446" checksum="D669E4C395E51860" modified="2011-01-11" version="1">MVECPICAKPIASGDINRHLDHDHFELRSSNHSEPKPQPTVTGAQSTLNFQRTVAGTQRTDAQNKTTGGAPKAHKRPHATNNLTSLHNEHLSQSQSSSSPSSSAPTSSVLSLSDHRKKSRLNPPSDSFVKSSLPTPSDSDPSNSNKPLAERVRPKTINEFQGQGHLKSALQMIMTSKNSIILWGPSGSGKTTLARCLKDELNRREEEAESSSQKNKKKKYEFFELSATDFSTSEWKKIIDQNHLYSASSNTSNTSGNGSNQKISSFYKSLNSAVTSIPIIFIDEVQRLNKSQQDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KWJ5</accession>
    <name>E3KWJ5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_14628</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178316">
        <property type="protein sequence ID" value="EFP88662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003333033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KWJ5"/>
    <dbReference type="GeneID" id="10541453"/>
    <dbReference type="KEGG" id="pgr:PGTG_14628"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14628"/>
    <dbReference type="HOGENOM" id="CLU_042830_0_0_1"/>
    <dbReference type="InParanoid" id="E3KWJ5"/>
    <dbReference type="OrthoDB" id="10308296at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP88662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="379" mass="42085" checksum="72CF8CEB4DBC0E09" modified="2011-01-11" version="1">MSPSQPSDALAEALLDLSRKCQELRQQKGHKQEGKATDLKIRQDSLSQIQTTLLPAARNSVVELYKALDLALKKSASPQPQYNDALAIVPSLSKALVNLSLSANNLDPKDEKFGLNDQNSGPLKKFRIENLQQEISELISIFLSDVFEEISELLSSGDFTNSGHDGAPAQPHASIDMVEDSFNRIIHLVDLSDFGLLQSLWQEDEEEVGHLLVIINREVSIAQHPDLLDDQGFSHKVTPRLVKLLEQCNPLIKSCRGFYRKIASADLFTISEKKSTVELESIVHHSAGIKQSLQHIVHALTSAAVLQRVEQVRGTQSHLHQFKKSVDDTLETLASHMTPSDPSAKVDEVMDKLFGHFKSTLYPAISQFTSDFSEFESSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>E3KWU0</accession>
    <name>E3KWU0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Multifunctional fusion protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="8">Delta-1-pyrroline-5-carboxylate dehydrogenase</fullName>
                <shortName evidence="8">P5C dehydrogenase</shortName>
            </recommendedName>
            <alternativeName>
                <fullName evidence="8">L-glutamate gamma-semialdehyde dehydrogenase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="7">L-glutamate gamma-semialdehyde dehydrogenase</fullName>
                <ecNumber evidence="7">1.2.1.88</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_14723</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-glutamate 5-semialdehyde + NAD(+) + H2O = L-glutamate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:30235"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="EC" id="1.2.1.88"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.88" evidence="7"/>
    <dbReference type="EMBL" id="DS178316">
        <property type="protein sequence ID" value="EFP88757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333176.1">
        <property type="nucleotide sequence ID" value="XM_003333128.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KWU0"/>
    <dbReference type="FunCoup" id="E3KWU0">
        <property type="interactions" value="171"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KWU0"/>
    <dbReference type="GeneID" id="10541708"/>
    <dbReference type="KEGG" id="pgr:PGTG_14723"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14723"/>
    <dbReference type="eggNOG" id="KOG2455">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_4_1_1"/>
    <dbReference type="InParanoid" id="E3KWU0"/>
    <dbReference type="OMA" id="PNAVRNT"/>
    <dbReference type="OrthoDB" id="5322683at2759"/>
    <dbReference type="UniPathway" id="UPA00261">
        <property type="reaction ID" value="UER00374"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003842">
        <property type="term" value="F:L-glutamate gamma-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010133">
        <property type="term" value="P:L-proline catabolic process to L-glutamate"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07123">
        <property type="entry name" value="ALDH_F4-17_P5CDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000006">
        <property type="entry name" value="1-pyrroline-5-carboxylate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000005">
        <property type="entry name" value="1-pyrroline-5-carboxylate dehydrogenase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016160">
        <property type="entry name" value="Ald_DH_CS_CYS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005931">
        <property type="entry name" value="P5CDH/ALDH4A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050485">
        <property type="entry name" value="Proline_metab_enzyme"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01236">
        <property type="entry name" value="D1pyr5carbox1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42862">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42862:SF1">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE DEHYDROGENASE 2, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00070">
        <property type="entry name" value="ALDEHYDE_DEHYDR_CYS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0520">NAD</keyword>
    <keyword evidence="3 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5 7" id="KW-0642">Proline metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="9">
        <location>
            <begin position="64"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP88757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="555" mass="60295" checksum="B64621D6031C263C" modified="2011-01-11" version="1">MASNACPTLATFKLPEIPNEPNQHYEPGSSSRIALDAAIKEMRAGMPYSVGPVIDGVELHNGPTVAQVLPHDHKTVLCNFHTASPELITKSIEGCLKAKAQWEALPFNDRSAIFLKAADLLSSPRWRYKIMAATMLGQGKNAWQAEIDAAAEMCDFFRFGVKYAEEMYAQQPPKNSAGVWNRVEYRPLEGFVYAISPFNFTAIGGNLAAAPALLGNVAVWKPSPMAVYANYLTYKLLEEAGLPKGVIQFIPVAGADVEPACKQIFGHPEFASLHFTGSTAVFRKLWSDIAQGVSTATLKSYPRVVGETGGKNFHLIHSSAPVRPTVLQSIRAAFEYSGQKCSALSRLYVPESIWPEFSKVLKEEVAKLKVGSPEEWSTFLGPVISQGSFDKITGLIQSAKDAGDEVIIGGKADASKGYFIDPTVILTRNPRSITMTEELFGPVITTYVYPDAEFEQTCDLIDSSSAYALTGSIFAADRHAVVLASNKLRRAAGNFYINSKCTGAVVGQQPFGGSRASGTNDKAGSINLLYRFVNARSIKEEFLPIEEAHYPSNLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3KXG0</accession>
    <name>E3KXG0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_15121</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178318">
        <property type="protein sequence ID" value="EFP88918.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333337.2">
        <property type="nucleotide sequence ID" value="XM_003333289.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KXG0"/>
    <dbReference type="STRING" id="418459.E3KXG0"/>
    <dbReference type="GeneID" id="10542196"/>
    <dbReference type="KEGG" id="pgr:PGTG_15121"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15121"/>
    <dbReference type="eggNOG" id="KOG0713">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_487562_0_0_1"/>
    <dbReference type="InParanoid" id="E3KXG0"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43948">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43948:SF10">
        <property type="entry name" value="MRJ, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="14"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="294"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="135"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="192"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="306"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="364"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="390"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="417"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="431"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="453"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="501"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP88918.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="560" mass="62250" checksum="7EFC7A148BCAF7A8" modified="2012-04-18" version="2">MTNSNSSSSTQPRTYYTLLGIDQDANPKEIEKAYKRQALIHHPDRNPNDSQSQSTNMFQKLAEAYEVLRDPIKRRKYDLKVANTIALHSDPATAHSRPSPGNAFPNRPASQQSSRPTNQPKRSSTFDLFAYPGEVPRYVPSPSRRPASTAPAFRPRSGSSAQSAEPMTSASINPNPSQLHRHCSIPAASPAGRHHHHQHHHQSPAQHKPFTQKINLKDLDSLDFLGLHPQSQNSRPSNIPSSALRAQARPTPEAANSSFHPTHSRDISSSAPDPYLTFERAWGQSLDGIVDGIADRQPDHRRYSNRKSNTEPISLNSKPPSVHRSATVRSADSDYRSSSSSFEHSTRRPRSAGGNRHADWLPSPNCTTSPQRLSSRSVQEPRSSLDDDLLYLPSSAYSSQNRRMSTGNNESFYHQPTTHHAHQQPRLYRHASNTGITGSSIRGTHGVGVRGASTHSQTSSPTSSSTDCPASSHPMAPNSRRSRASTLMPDYPPLENKHDPQTQTQTQSHGLSREFREVIIRFERERDGSTRLSKEIKSRTIDLDGTVHDRYRRQRAAAVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>E3KXM8</accession>
    <name>E3KXM8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">aspartyl aminopeptidase</fullName>
            <ecNumber evidence="4">3.4.11.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_14931</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of an N-terminal aspartate or glutamate from a peptide, with a preference for aspartate.</text>
            <dbReference type="EC" id="3.4.11.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the peptidase M18 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.21" evidence="4"/>
    <dbReference type="EMBL" id="DS178319">
        <property type="protein sequence ID" value="EFP89090.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333509.2">
        <property type="nucleotide sequence ID" value="XM_003333461.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KXM8"/>
    <dbReference type="FunCoup" id="E3KXM8">
        <property type="interactions" value="320"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KXM8"/>
    <dbReference type="GeneID" id="10546559"/>
    <dbReference type="KEGG" id="pgr:PGTG_14931"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14931"/>
    <dbReference type="eggNOG" id="KOG2596">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019532_2_0_1"/>
    <dbReference type="InParanoid" id="E3KXM8"/>
    <dbReference type="OrthoDB" id="9880441at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05658">
        <property type="entry name" value="M18_DAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.250.10:FF:000001">
        <property type="entry name" value="Aspartyl aminopeptidase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.250.10">
        <property type="entry name" value="Aminopeptidase i, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001948">
        <property type="entry name" value="Peptidase_M18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023358">
        <property type="entry name" value="Peptidase_M18_dom2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002759">
        <property type="entry name" value="PRK02813.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28570">
        <property type="entry name" value="ASPARTYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28570:SF3">
        <property type="entry name" value="ASPARTYL AMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02127">
        <property type="entry name" value="Peptidase_M18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00932">
        <property type="entry name" value="AMINO1PTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101821">
        <property type="entry name" value="Aminopeptidase/glucanase lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="8 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="6 11" id="KW-0645">Protease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP89090.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="493" mass="53998" checksum="81627D5F83106640" modified="2012-04-18" version="2">MFHHPSPPTIVDKFLTFINQSPTPFHAVSNAIKRLESAGFKRLKERDQWNGLIQKGGKYFVTRNQSSIIAFAVGGAYEPGNGMAIVGCHTDSPCFKIRPVSKQTKMGYLQVGVETYGGGIWPTWLDRDLGIAGRVIVKDQPSGSTDSDSFVSHLVHIPRPILRFPTLAIHLERTQTDQLKYNPETQMIPILSMATEQLNETAKPTESASAISKSALSTQENHHPLLLSILAEALSEKLGVPVKESEIHDLELSLFDTSPSIVGGGRAEFIFSPRLDNLFSSFAAFEALAQSVEGGPNSKGLEQSALIRTIALWDNEEIGSVSHQGAESNFLEAILTRVSSSFMNQPSPALTEQTLAASFLLSCDMGHAVHPCYPEKHEQNHRPLINKGPAIKTNAKQRYASTAATTFLLRQVAAIAKVPLQEYEVRNDMACGSTIGPLVSKIGLRTVDIGCPQLSMHSIREQAGCQDLVYLTALFENFFEHFQTVDSRLSTGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3L0Y3</accession>
    <name>E3L0Y3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_16486</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178330">
        <property type="protein sequence ID" value="EFP90208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334627.1">
        <property type="nucleotide sequence ID" value="XM_003334579.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L0Y3"/>
    <dbReference type="STRING" id="418459.E3L0Y3"/>
    <dbReference type="GeneID" id="10546350"/>
    <dbReference type="KEGG" id="pgr:PGTG_16486"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16486"/>
    <dbReference type="HOGENOM" id="CLU_681748_0_0_1"/>
    <dbReference type="InParanoid" id="E3L0Y3"/>
    <dbReference type="OMA" id="MYTSIAV"/>
    <dbReference type="OrthoDB" id="10420250at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34785">
        <property type="entry name" value="ECA1 GAMETOGENESIS RELATED FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34785:SF3">
        <property type="entry name" value="ECA1 GAMETOGENESIS RELATED FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174298" evidence="2">
        <location>
            <begin position="23"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="343"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP90208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="404" mass="42081" checksum="94FF76BC8E757EDB" modified="2011-01-11" version="1">MTRISYLFVAVAISVLAGHSNCSPDEDSQGYGLFPDLYNTLEPLPLLGGLAGGLLGSLDSGPTPENPTPGEPVALKHNQPSVRANNRRRQRAIVLNILDDLSQPGYFPNPASRISSKSGRHKQKPIIGPPMGPALLPQPPSPRADDLAPLPVAASPETVPPPMDYPPVDQTPAMNDRPIPGPPVDDSPQPPAEDASPPVHVLPPFLAGSRMPGVSTIPLKKPAPKTKRDVSDAVTAVPPIMETPAIPSIPSHPEIPSIPAIPAIPSIPAIPAIPSIPAIPAIPSIPAIPSIPSLPSSSTTYPTFMGAMDAFRMLVANGMPLQRQIANGPVSAEHIPAGPRPALGAIHPPSSASAHTKRSVPDGLVNGSPPLSPLRFGRLFSSFPSLSMPSIPSIPSFSPFSNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3L1P2</accession>
    <name>E3L1P2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic elongation factor-2 kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178333">
        <property type="protein sequence ID" value="EFP90467.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334886.2">
        <property type="nucleotide sequence ID" value="XM_003334838.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L1P2"/>
    <dbReference type="GeneID" id="10530363"/>
    <dbReference type="KEGG" id="pgr:PGTG_16054"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16054"/>
    <dbReference type="HOGENOM" id="CLU_1482709_0_0_1"/>
    <dbReference type="InParanoid" id="E3L1P2"/>
    <dbReference type="OrthoDB" id="10270247at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90467.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="182" mass="20093" checksum="4D88B902F3228D15" modified="2012-04-18" version="2">MVTCLKCTRFVTQVEFGWCRECLNFVGLMPGNMANQLPSQPANHYNIIPPAPTSSILQVPPAIPTPPAASLAQCAGTNAYLRNVGKAKRTKNPAPTPYSRNPKPSTSAPPAESPIRMFKCGISFFQDEKLKKTGMMSYMQNIDTSIPKLYETLQHQLWLLFSPELLRKGLVESLPSIVVDKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3L217</accession>
    <name>E3L217_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_16618</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178335">
        <property type="protein sequence ID" value="EFP90592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335011.1">
        <property type="nucleotide sequence ID" value="XM_003334963.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L217"/>
    <dbReference type="GeneID" id="10530492"/>
    <dbReference type="KEGG" id="pgr:PGTG_16618"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16618"/>
    <dbReference type="eggNOG" id="KOG0800">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112996_0_0_1"/>
    <dbReference type="InParanoid" id="E3L217"/>
    <dbReference type="OMA" id="VIRCMLR"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:001781">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051834">
        <property type="entry name" value="RING_finger_E3_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931:SF3">
        <property type="entry name" value="RING ZINC FINGER-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931">
        <property type="entry name" value="SI:CH211-59O9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173009" description="RING-type domain-containing protein" evidence="5">
        <location>
            <begin position="27"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="63"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP90592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="141" mass="15440" checksum="D0E3CC901A549B02" modified="2011-01-11" version="1">MKLLRFTSKFLSIALVTLLFIKVSQEAAFPPGSSSGLENVSESEGKAIEVTGEPSFSSSETTCVVCLDDLHNNKEKGPIGTLEPCGHSFHRACADNWLQTKGTCPICRSEYAPWKFKYKEDPTAYQVNGQGSPLFVPHTPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3L2W8</accession>
    <name>E3L2W8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17092</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178338">
        <property type="protein sequence ID" value="EFP90893.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335312.2">
        <property type="nucleotide sequence ID" value="XM_003335264.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L2W8"/>
    <dbReference type="GeneID" id="10530801"/>
    <dbReference type="KEGG" id="pgr:PGTG_17092"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17092"/>
    <dbReference type="HOGENOM" id="CLU_621315_0_0_1"/>
    <dbReference type="InParanoid" id="E3L2W8"/>
    <dbReference type="OrthoDB" id="2505289at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="348"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="167"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="297"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="365"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90893.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="445" mass="48848" checksum="8AC3DCAB26ECED51" modified="2012-04-18" version="2">MAGTTTGESPRNETRIETDELIELEMTINNEEEDGDGDGDGGEGEEQDTSNQQLVKPPRRPPTFEAAFSQARSLTNHGRVNYQQALINNNHSSRGSLDSLALLPPPPPPPAQAENNLQSLRPTNSSIEQLHLTLPGQHDPDTHFLTSLVDSETTADQLIFSPHHQQLSLSPPTDSLLPIPRSPSPLLDEPNSSPPGLYGSSPPPDRSPSHESPPPSPPAPRTVRVSPPKNPTGPPRPPPLKLCLPPRPRRPAIETSQTPYNSPAGSNPSTPLISTSNLRPVSNQSNLLKRTLPSSSTDQHQNQNQNQNQTTTAKKQKINPLFLVSSTSKTPAENEKLKAWKRIQLLRAQRDQPQHLSSSGSTSKNSEELERLEKSTNSNSLGDTLSSAIQLRNQAQEYLDLIQKTQDALGEELVRVQLEESVLRHVRGIIADRLIKNQEWNNQNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3Q5</accession>
    <name>E3L3Q5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16371</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178343">
        <property type="protein sequence ID" value="EFP91180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335599.1">
        <property type="nucleotide sequence ID" value="XM_003335551.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L3Q5"/>
    <dbReference type="GeneID" id="10528844"/>
    <dbReference type="KEGG" id="pgr:PGTG_16371"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16371"/>
    <dbReference type="HOGENOM" id="CLU_018307_0_0_1"/>
    <dbReference type="InParanoid" id="E3L3Q5"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="237"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="344" mass="37666" checksum="536DFFCCC4232C74" modified="2011-01-11" version="1">MTTRRNTESHDLLPLTDPEAIIRSGNAERRRLSNLHAQPIAPLPFRPSPSEENMSDATATPGTQEQTTDSARTADATDISTAKDWFKAVLKIQHSTIAQAQEDRRQALDDRRADRQIFMAAQQANAARIGRLEDLLLAMNIKNEVATRPEQSAPGRINLQKFRTSDGPTYRGPFQETEPFLRWIHGVQIFFDTKDVSNSADRIKIIGNLIAETNLQSIPNYQAPTKPADYSAPRTWSSPSGPSSTPGKPTQPPAGRPSTRAASVAGVTDVTPLEAQVSALQLEAAIREDGRWDTYFDDKGCYPSMDLAAVAALEDLDKQLLANEIEKAEQADLADAIAGRPGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3T4</accession>
    <name>E3L3T4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_16400</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178343">
        <property type="protein sequence ID" value="EFP91209.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335628.2">
        <property type="nucleotide sequence ID" value="XM_003335580.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L3T4"/>
    <dbReference type="GeneID" id="10528872"/>
    <dbReference type="KEGG" id="pgr:PGTG_16400"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16400"/>
    <dbReference type="HOGENOM" id="CLU_1171110_0_0_1"/>
    <dbReference type="InParanoid" id="E3L3T4"/>
    <dbReference type="OrthoDB" id="10285058at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP91209.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="192" mass="22019" checksum="A164E5F46DA179C0" modified="2012-04-18" version="2">MTKLEQRLNESQELLEKLKKIPNPRQPAQTHLKVVSASLLENQPKIWTSPCYKRHLRLNENKMGLSKWCLLFYGSYSLTPTIPIILPQKHFVDRKDQRGRLDSIGISNTLNASIHEDWEEQSGFHTPTLSIHEDETDQSGHDQVEVTSTTATIATMQVPAEEEEGRRIRDSLLLPRVTALDEAINSWRKKIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>E3L472</accession>
    <name>E3L472_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178345">
        <property type="protein sequence ID" value="EFP91347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335766.1">
        <property type="nucleotide sequence ID" value="XM_003335718.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L472"/>
    <dbReference type="STRING" id="418459.E3L472"/>
    <dbReference type="GeneID" id="10529178"/>
    <dbReference type="KEGG" id="pgr:PGTG_17001"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17001"/>
    <dbReference type="eggNOG" id="ENOG502S6K1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008138_2_0_1"/>
    <dbReference type="InParanoid" id="E3L472"/>
    <dbReference type="OMA" id="CETAMIC"/>
    <dbReference type="OrthoDB" id="2498162at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266">
        <property type="entry name" value="CHROMOSOME 15, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266:SF1">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="804"/>
            <end position="878"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="805"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="837"/>
            <end position="848"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="878" mass="98636" checksum="7CC54C3F53817841" modified="2011-01-11" version="1">MVQEAKSELERFLKLHAQHNNAEKLFYHLEKAAGCLIPLEELTNQLGFTRKADGQKIKALIRRAHFVRFPERHASELTEAQMLAQLGLSSTSAEQIVDASPEVDWSTEIVQQGFDAEYQGHDLIVIPTLETLHHYAGQWREDAYKAPYTSIISPTMSGKTRLLKELAKHVCVVYICLRKEGSTGQPPRSDLARHFIPEKGQLNMIQHYSHLLIAILKTVYDFFKRPDISTKSLEDQLSEWFDYSFQIKSTSKVEFNTKVENELNNLKMASSSESKLTYDDIQIGDLPTRQKLATAVSDVDEIMKSQGRGNLKVLLAIDEASNLIDHVNKQLNIPYFRVFRRALSTIPSLMGFFSVFTDTTSRVANFNPALDRDSSTRFHGRGVDLFAPIYKISTLDVFVPKDKPSSWNDLLSPERLFSYGCPFYGLYFKGANKKNPKGAVQTTAMIAQAKLLFKTQSASPGVLSSPQCFAILGSIIQTRVSLHAPINSELVASHAAHCMFIDSKREMIVSDYPPQFVYASAANRVLASDDKRWIECIDALASAVRKGLVALGDAGEMATRLILIRAMQKTEPIPHKNLDSIPNGYSVRLKDFLQTLSGKNPDIMNFDCSDEEYRNKLLDHGQVFFNHFTRISYTPSATDFLELLYRGVAVQCKDRQPGLDELFTIYLAPPSTSHSSKLDLSNITFCGIQTKNRTGSVRMDESHHWSKSFAKIEGINNPYLILLFSLKATSSQVTWKPPELKDDTQRVAYQFLGLGEIDCLTSEMRAGLERLITAIPDDLLNLHDNCNEKTKDWIKQLTPVFYPSAPEPVPSQKPSKPPAPEPPQKPAQLPAAKKQSNRTQQVIKQTCVPSPKPSKRPAPEPPQKPYNLRAPKGQSSRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3L4B9</accession>
    <name>E3L4B9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Conidiation-specific protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17350</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178346">
        <property type="protein sequence ID" value="EFP91394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335813.1">
        <property type="nucleotide sequence ID" value="XM_003335765.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L4B9"/>
    <dbReference type="GeneID" id="10529308"/>
    <dbReference type="KEGG" id="pgr:PGTG_17350"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17350"/>
    <dbReference type="eggNOG" id="ENOG502S75W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107705_2_1_1"/>
    <dbReference type="InParanoid" id="E3L4B9"/>
    <dbReference type="OMA" id="NEMRGYK"/>
    <dbReference type="OrthoDB" id="5419162at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018824">
        <property type="entry name" value="Conidiation-specific_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052670">
        <property type="entry name" value="UPF0654_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36576">
        <property type="entry name" value="UPF0654 PROTEIN C11D3.01C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36576:SF1">
        <property type="entry name" value="UPF0654 PROTEIN C11D3.01C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10346">
        <property type="entry name" value="Con-6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="83" mass="9094" checksum="8BA0D68410D4F020" modified="2011-01-11" version="1">MANPGNVAGGLKATLNNPHVSDEAKEHAEHRLDTEGFKSGSAQETVHVKDPDNVKRGMKAALHNPNVSEGKKEELRHKLDEQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3L4D6</accession>
    <name>E3L4D6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17367</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178346">
        <property type="protein sequence ID" value="EFP91411.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335830.2">
        <property type="nucleotide sequence ID" value="XM_003335782.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L4D6"/>
    <dbReference type="GeneID" id="10529325"/>
    <dbReference type="KEGG" id="pgr:PGTG_17367"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17367"/>
    <dbReference type="HOGENOM" id="CLU_674616_0_0_1"/>
    <dbReference type="InParanoid" id="E3L4D6"/>
    <dbReference type="OrthoDB" id="10286211at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="42"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91411.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="408" mass="46889" checksum="2B1FAFDE2563C878" modified="2012-04-18" version="2">MRWPEEYSSDSSSSSEDDFVMHDWFPLGASGRSAPISIPRKASWSSTSSSEAASPREAGHSMSRSSSPIIIPKFTDRYPYHPNYDLSVGGRSQVSYDRDTPSPSGASQAEHTWDDDMQWTPTSKHRFANGHFSDAELSTVSSPASPSRDSMSSSSTNSGHEQDDVGSSRMKAIDYFEYFSKEKPQLDIDLHYRIDRSIIEQYSKNAGEYYELKQQGFSRVSIKHKDLPFGLATISDAQPGRASLVLYHKNHDILLKDEFQSMYKKLIIALSEKHAEDFNIEVFNDYEYQTEQRRLLSWLDKEIYAPYDSLPVIGLSRYSFESGPTDQFLLKPNQLHLIRYFSEALWHPTDEEFIEQTANPLIESYKSRPKIPQVETMHPPKYLEEQKSSHSKDELKESWNFIYGLWYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3L5D9</accession>
    <name>E3L5D9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17764</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178352">
        <property type="protein sequence ID" value="EFP91764.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336183.2">
        <property type="nucleotide sequence ID" value="XM_003336135.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L5D9"/>
    <dbReference type="GeneID" id="10531707"/>
    <dbReference type="KEGG" id="pgr:PGTG_17764"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17764"/>
    <dbReference type="HOGENOM" id="CLU_186282_0_0_1"/>
    <dbReference type="InParanoid" id="E3L5D9"/>
    <dbReference type="OrthoDB" id="2508506at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91764.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="93" mass="10310" checksum="5FC071B6C2F4C702" modified="2012-04-18" version="2">MTYTRTNQPHDRHIHEPIAKSTMSCHNTVEEVAGSPPTPTWPDGPSTITRPDGPSDHPKRSSVSQPSTVPHLQVNQQQQPHRMHLVIPATLPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3L5Z3</accession>
    <name>E3L5Z3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18419</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178355">
        <property type="protein sequence ID" value="EFP91968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336387.1">
        <property type="nucleotide sequence ID" value="XM_003336339.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L5Z3"/>
    <dbReference type="GeneID" id="10531920"/>
    <dbReference type="KEGG" id="pgr:PGTG_18419"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18419"/>
    <dbReference type="HOGENOM" id="CLU_2039218_0_0_1"/>
    <dbReference type="InParanoid" id="E3L5Z3"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="121" mass="13509" checksum="51171A675D418A6E" modified="2011-01-11" version="1">MHERPRRHVECVTLDTSQTPCSCPPPGSRTSPVNSTARQPLPTLTLSPPLLLPIPTNHAPFLLPLSSIEPPSSLSRTFSILRGRLMHLFNPLSHFTSPIQCILFLGLHLPNFLFRHLLPTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>E3L648</accession>
    <name>E3L648_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="9">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_17854</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">ATP-dependent DNA helicase involved in DNA damage repair by homologous recombination and in genome maintenance. Capable of unwinding D-loops. Plays a role in limiting crossover recombinants during mitotic DNA double-strand break (DSB) repair. Component of a FANCM-MHF complex which promotes gene conversion at blocked replication forks, probably by reversal of the stalled fork.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with the MHF histone-fold complex to form the FANCM-MHF complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the DEAD box helicase family. DEAH subfamily. FANCM sub-subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="9"/>
    <dbReference type="EMBL" id="DS178356">
        <property type="protein sequence ID" value="EFP92023.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336442.2">
        <property type="nucleotide sequence ID" value="XM_003336394.2"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L648"/>
    <dbReference type="GeneID" id="10531976"/>
    <dbReference type="KEGG" id="pgr:PGTG_17854"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17854"/>
    <dbReference type="eggNOG" id="KOG0354">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002513_5_2_1"/>
    <dbReference type="InParanoid" id="E3L648"/>
    <dbReference type="OrthoDB" id="164902at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000400">
        <property type="term" value="F:four-way junction DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045003">
        <property type="term" value="P:double-strand break repair via synthesis-dependent strand annealing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18033">
        <property type="entry name" value="DEXDc_FANCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12091">
        <property type="entry name" value="FANCM_ID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18801">
        <property type="entry name" value="SF2_C_FANCM_Hef"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000861">
        <property type="entry name" value="Fanconi anemia, complementation group M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039686">
        <property type="entry name" value="FANCM/Mph1-like_ID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044749">
        <property type="entry name" value="FANCM_DEXDc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14025">
        <property type="entry name" value="FANCONI ANEMIA GROUP M FANCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14025:SF20">
        <property type="entry name" value="FANCONI ANEMIA GROUP M PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
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    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="272"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="599"/>
            <end position="763"/>
        </location>
    </feature>
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            <begin position="25"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="144"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="199"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="792"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
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        </location>
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            <begin position="33"/>
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        </location>
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        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
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        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
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            <begin position="210"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="796"/>
            <end position="808"/>
        </location>
    </feature>
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        <location>
            <begin position="1062"/>
            <end position="1076"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1117"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="1214"/>
            <end position="1228"/>
        </location>
    </feature>
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        <location>
            <begin position="1229"/>
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        </location>
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        </location>
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        <location>
            <begin position="1317"/>
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        </location>
    </feature>
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        <location>
            <begin position="1416"/>
            <end position="1425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1426"/>
            <end position="1438"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00009889"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU367027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP92023.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1464" mass="162798" checksum="66E9F558EB8CA69A" modified="2012-04-18" version="2">MSDLDDYFDDDIDAAAFDAVLSAATSVPPPTRQPVVVPTTRQPASITRQPASTTRQPASTTRQSGPKDRQPALNIRHPTPPPVNREPIAASSSTIDDCFDDDIDAAAFDAVVNAATSAQPTTRQALGGSGLTQKKYTQTTIFGASQVPAKPSRPSKQPTSSQYQPPVPKATQQEPKVKAVKKWDNLRAIKDVLPDINQKGKKRKKSKFADDDDEEEEMMDDEEVDSDTDLLNQALQETNHKKVPMKLQSDSEAIKTWIYSINKPKRKYQYDIVARALYNNCLVSLPTGLGKTFIAAVVMLNFYRWYPRGKVIFLAPTRPLVQQQIEACHSIAGIPSVDCVSLTGTTSATKRAVAWENKRVIFATPQTVANDLRRGSLRPEDIICLVVDEAHRATGAYAYCIVVKLLMRFNPHFRVLALTATPGSDPERVQAVIDNLHIGHIEVRTEESMDIVPYTKNKEIVLEKIPLTPELTSIRDKWGKLMLPLIKQCGALFHGSKEPAYIHAFSITSAQMHIPKEKSYLRPNLAVLSKMATAMAKLLEYSITLAYEKMQELKEESGKCAAGVCSQVGYKSLMTEIRDLTQSPDFVPHPKMEKMKALTLEFFINAQDQGKSSRVMIFCHFRDMVTDIVNYLNQEKPIIKASAFVGQSNDVRGNRGMNQKTQNETIQRFKNNEFNVLVATSIGEEGLDIGALDFIICYEAQKSPLRMLQRLGRTGRNEDGKVIVLIAEGREDKNWEKAQDQYQHVQNALLSKKVLELYEDCERLVPEGVRPTAVDKEIDVQPYIADQVELAVSPSGRKKKDKPTKRKRPGAEDIPAGGIFGFVSAKDYAVPKSKADSLRQRTEAALLTAEEAAILKQQWSKNITVAPSPIDPENLKIEYFPTKSALGSRLESVPQNKRSARNITRSAKYTRIMNQCDSYVHDDAKYEAWREDTIARLESKNVTWWPPELRGMGAQKPLRLCSDGPKSIRRRWTNSKDAGASTPVPQPRSPTIAAFSSPRSSSPRLLDEFGEGAPRAISPKGKSKKASQLDEPPSSHDDLPDFEDFIPSMKTSERLLPPPSIASPSPLASTSANAPPHARPDSTRPGQSSSFQRLAAFKSSVSEPAPFQEAVPFRQEFPNNAPVTPSGPSNDRGVTKKRLVGPARFQDDDDHSPLLFRALTHNNKQAPPISPSEYENGDDFLRPEILPNTQMPRSKRIQKSVGFGGKQKRIIAPESDDDDDDHKDDDGDVMVGEDGPDGPQSHKKSERKSDRHVKTRKDGASSSKGKGKQRQQKSSHSTRGNEWALKTGLFDLEASESDTSSSSEEEGEEGGSGDGQRKKKKRRKKGKGGRKKKKRSSSEEIESENSIDRDFLVNTSSSKIQDDCSPDKSMTSFYRASILMMSQPLSVVKTKLGKRAPSPQPRPNNHPLHHHHRSNPPRQSSSRRPISSEHDHPDDRFSFDSFCVPDEETIEYEDEDCHGIPSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3L650</accession>
    <name>E3L650_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17856</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP92025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003336396.1"/>
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    <dbReference type="GeneID" id="10531979"/>
    <dbReference type="KEGG" id="pgr:PGTG_17856"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17856"/>
    <dbReference type="HOGENOM" id="CLU_1475842_0_0_1"/>
    <dbReference type="InParanoid" id="E3L650"/>
    <dbReference type="OrthoDB" id="10284653at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>E3L696</accession>
    <name>E3L696_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_18161</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
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        <property type="term" value="F:zinc ion binding"/>
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        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
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        <property type="entry name" value="Zn2Cys6_DnaBD"/>
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        <location>
            <begin position="30"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
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    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP92071.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
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    </protein>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="138" mass="15633" checksum="8E9D818D40CC2AFE" modified="2011-01-11" version="1">MTKIFRNSFGIISKSSFGVDFEIMASRNFFESISNNYREVFERFSESPSEHIAKCTWTKVQDVAPCGRVFRNISERASEMFRNAAPVVYKKLPEKFPDQASAAGPNGNLPSTSFDGQPTQIRRFALRQQGQPPKVKNC</sequence>
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    <accession>E3L8J4</accession>
    <name>E3L8J4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
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                <person name="Schein J.E."/>
                <person name="Zhong S."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18787"/>
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        <location>
            <begin position="1"/>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="10"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="117" mass="13580" checksum="0E77FA8D995128CE" modified="2011-01-11" version="1">MAQNLPQREEAEEGEEEELEVVIDKAPKAFTGEKPLSNNQLTLIDMLIASKKVYMNAKAAEEYEILPQLIEQSIKIFQTVTTFLGWKETRAKIDNWHPHKEKKVAEFWANHAKEQKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3L980</accession>
    <name>E3L980_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_19210</name>
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    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
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            <taxon>Puccinia</taxon>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178382">
        <property type="protein sequence ID" value="EFP93105.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337524.2">
        <property type="nucleotide sequence ID" value="XM_003337476.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L980"/>
    <dbReference type="GeneID" id="10543170"/>
    <dbReference type="KEGG" id="pgr:PGTG_19210"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19210"/>
    <dbReference type="HOGENOM" id="CLU_1587322_0_0_1"/>
    <dbReference type="InParanoid" id="E3L980"/>
    <dbReference type="OrthoDB" id="2496723at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP93105.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="168" mass="19690" checksum="3F78FC65C9F3D733" modified="2012-04-18" version="2">MLSLGLHPEDDLFLDEQSRVHNEFHSLLTCSDNPQTNSFYNNISSKSKSMIKHPTQQRILEALNFDPRGDEEQKSKMMTRAAVQVIIAYYFKSNTGKFLQVFPNESIFISSLVRITKRTGERFLHEYFMLNGFKEMANLKLFPWRNYFLKTSNNLVILKRLIQSQRGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3LAX0</accession>
    <name>E3LAX0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19664</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178404">
        <property type="protein sequence ID" value="EFP93695.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338114.2">
        <property type="nucleotide sequence ID" value="XM_003338066.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LAX0"/>
    <dbReference type="GeneID" id="10534986"/>
    <dbReference type="KEGG" id="pgr:PGTG_19664"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19664"/>
    <dbReference type="HOGENOM" id="CLU_043067_0_0_1"/>
    <dbReference type="InParanoid" id="E3LAX0"/>
    <dbReference type="OrthoDB" id="2503913at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93695.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="351" mass="40345" checksum="E425D0D9FBDADFF9" modified="2012-04-18" version="2">MSILFPDTLQPPSDTLQPPSDPTPAMSRGDSLVDFAKFMMGCHGVNFPLPPEPTVEEIHGREKYVAASLLLGACPNPSDYHQNQLLCHPKFTSIDKARCDHDFHSHGFNRITFEWRKPNWTDSDWNNCTADILCDNWGIWVVNERKVSTFSFLDAKAALLEWLVSRNVNFTRIRKQIEEGKDIVDDNFEQMDGWHMLYRKKVAQSRFKTALSIFPDQSELLAMFKDPDTVSDYEESEDITVLPVRIIPLWRSSMLTALVREIDLATIQLAAREKKTAIVRWISRSGERHVTDDEKPYQRIPLGLHIKAYGQDFLEKNSILVHHQLKICKDNQPYSLADVLGYLKKITRSAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3NXL2</accession>
    <name>E3NXL2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ribonuclease Z</fullName>
            <ecNumber evidence="4">3.1.26.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_20265</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.</text>
            <dbReference type="EC" id="3.1.26.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RNase Z family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.11" evidence="4"/>
    <dbReference type="EMBL" id="DS989949">
        <property type="protein sequence ID" value="EFP94311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338730.1">
        <property type="nucleotide sequence ID" value="XM_003338682.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NXL2"/>
    <dbReference type="STRING" id="418459.E3NXL2"/>
    <dbReference type="GeneID" id="10527438"/>
    <dbReference type="KEGG" id="pgr:PGTG_20265"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20265"/>
    <dbReference type="HOGENOM" id="CLU_940529_0_0_1"/>
    <dbReference type="InParanoid" id="E3NXL2"/>
    <dbReference type="OrthoDB" id="527344at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042781">
        <property type="term" value="F:3'-tRNA processing endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047151">
        <property type="entry name" value="RNZ2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12553">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12553:SF49">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="97"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP94311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="296" mass="32167" checksum="E0EDA8586B9CF27B" modified="2011-01-11" version="1">MGCDHLVGRDSSRRDGLYILSSGGIPPNEMGISSGGIPVDASDSSSPSLINRALFTRRQGMSVHVNEFKPTSTLQQVFQDHLIRVYALGNDRKDQTHLLMNRKRSPDSTAISDLSHKRTKLEPGNSKPPTGLQPVLNSHNALPEVIDDMFRASGTSRPLGKKNTTPAYSYQRLQEPDEPLTTDPVSYLVIGPRLRGKFLPEKAKQLGVKPGPKFAKLVNGESVQVNGETVVTSDMCVEGGSAGLAFFISSIGSMEQLGRTTLVDPACISRVSDGANLCAVYHLVHEDVVLDERYIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3NXY6</accession>
    <name>E3NXY6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20391</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS989996">
        <property type="protein sequence ID" value="EFP94435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338854.1">
        <property type="nucleotide sequence ID" value="XM_003338806.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NXY6"/>
    <dbReference type="GeneID" id="10527727"/>
    <dbReference type="KEGG" id="pgr:PGTG_20391"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20391"/>
    <dbReference type="HOGENOM" id="CLU_1595360_0_0_1"/>
    <dbReference type="InParanoid" id="E3NXY6"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP94435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="181" mass="20446" checksum="3965D3E0AA430ED1" modified="2011-01-11" version="1">MSFSRPLSIASSDATITGSGSDSDSVDESSSTDYGSVANYSPVDYDTVDKRSSALPADYSNFSPAFTRDSFSLEELHDFRILVTDELIRFARSISDSNETDRVQRLRLYSRIEDLFSTLHMHQLGPPMELRRALIQAKGKQEKKRDKEEMAVFHKEVEAQSEEVTEEYVSEIKALVEDQGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>H6QNT0</accession>
    <name>H6QNT0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_20638</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EHS62517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890834.1">
        <property type="nucleotide sequence ID" value="XM_003890785.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QNT0"/>
    <dbReference type="GeneID" id="13542511"/>
    <dbReference type="KEGG" id="pgr:PGTG_20638"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20638"/>
    <dbReference type="HOGENOM" id="CLU_1563634_0_0_1"/>
    <dbReference type="InParanoid" id="H6QNT0"/>
    <dbReference type="OrthoDB" id="10524451at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS62517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="171" mass="19100" checksum="FDF7E18188EE38D1" modified="2012-04-18" version="1">MTLVNPESYSTTDNQESRSKLSREPVFHLAKLAIPLIKISKLFFTKLSKCGLNKTRLPLFTEMASEQIESLANSLGQVTRDIIELGGLLPKADDGDATGQDFVKIADKLRSRYEAPLVALLLYVIPSIPDSDDGFPTQSYYRTWLVTWNTQRILATVNFINAAKLLDPNPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>H6QNW7</accession>
    <name>H6QNW7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_20628</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EHS62506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890823.1">
        <property type="nucleotide sequence ID" value="XM_003890774.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QNW7"/>
    <dbReference type="GeneID" id="13542531"/>
    <dbReference type="KEGG" id="pgr:PGTG_20628"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20628"/>
    <dbReference type="HOGENOM" id="CLU_3107457_0_0_1"/>
    <dbReference type="InParanoid" id="H6QNW7"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS62506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="51" mass="5790" checksum="2EB8B38BA103ACBA" modified="2012-04-18" version="1">MSQEVYCSDSKFFKRIKILLTVDEGRKGFSSSLSSFEPEKQYGKCGFNGKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPV2</accession>
    <name>H6QPV2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_20904</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EHS64290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890446.1">
        <property type="nucleotide sequence ID" value="XM_003890397.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPV2"/>
    <dbReference type="GeneID" id="13542997"/>
    <dbReference type="KEGG" id="pgr:PGTG_20904"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20904"/>
    <dbReference type="HOGENOM" id="CLU_1156878_0_0_1"/>
    <dbReference type="InParanoid" id="H6QPV2"/>
    <dbReference type="OrthoDB" id="6105938at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051865">
        <property type="term" value="P:protein autoubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047126">
        <property type="entry name" value="RNF141-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017907">
        <property type="entry name" value="Znf_RING_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109:SF3">
        <property type="entry name" value="RING FINGER PROTEIN 141"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109">
        <property type="entry name" value="RING FINGER PROTEIN 141-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00518">
        <property type="entry name" value="ZF_RING_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0732">Signal</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5012406961" description="RING-type domain-containing protein" evidence="7">
        <location>
            <begin position="16"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="8">
        <location>
            <begin position="130"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="80"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS64290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="240" mass="26449" checksum="C5B1E488A132508E" modified="2012-04-18" version="1">MDIVFLLLLAGVCFARELPDGSSLIEFREENTNFLPSLHSIDIPSAVIQRGSDGSSSCHVSAGNADLQGENSSLKEIVIQDGAGGRSPGKEVPTEISNSEDSPHYLKFDGGQVASHDLKLSGNAREAEMCSICLNSIPSTQDPVFYFGQECRHNFCNECIDNWLNQQRKQATCPVCRCPVEWTKELAAKLPAEELQFDQPCRSMMQIIRSLSREQVAILTALVLFFGILITIGQIVPNMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQ08</accession>
    <name>H6QQ08_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="6">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_20954</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="6"/>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EHS64491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890405.1">
        <property type="nucleotide sequence ID" value="XM_003890356.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQ08"/>
    <dbReference type="GeneID" id="13542626"/>
    <dbReference type="KEGG" id="pgr:PGTG_20954"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20954"/>
    <dbReference type="eggNOG" id="KOG2204">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_994445_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQ08"/>
    <dbReference type="OrthoDB" id="8118055at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000131">
        <property type="entry name" value="alpha-1,2-Mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050749">
        <property type="entry name" value="Glycosyl_Hydrolase_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742:SF103">
        <property type="entry name" value="ENDOPLASMIC RETICULUM MANNOSIDASE MNL2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS64491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="314" mass="35185" checksum="11918CED4410D187" modified="2012-04-18" version="1">MSQLPGMIAGGPAGNAFRTGANANAFGALIRSRLRALLSLVLASGLGLCFYLYFYRPILHDTPHLPVEEVMEPQIGKHDEMGGAVVPDLRAIGASPVENSENITVTQYAVFEPNKTYTDEELMGLPILHHTFEDALDPRNGFQPLRHSKYPWLTTTPTGTMLIDRLSRNRFPPPSSIDSSHGSSPPPIPIWPPRTILQEKHHSLQNPIPKDRIAGLNTSYIWKDRHRIPEGKMPKVQWSGFERPDWETPSDRDNRLERQGWVRRGFQHVWEGYKANAWGHDELKPLSGQFEDGFSGWGATLVDSLDTSGHSKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQ37</accession>
    <name>H6QQ37_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21016</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EHS64665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890371.1">
        <property type="nucleotide sequence ID" value="XM_003890322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQ37"/>
    <dbReference type="GeneID" id="13542739"/>
    <dbReference type="KEGG" id="pgr:PGTG_21016"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21016"/>
    <dbReference type="HOGENOM" id="CLU_2942865_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQ37"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="60" mass="6761" checksum="D4CCFE9DC7439CDB" modified="2012-04-18" version="1">MNKDISGLNEVTQEFYRLQREQIMLKLCKRSRAAAKQARVPSTQASCSTPIELMLSSSNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQ66</accession>
    <name>H6QQ66_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21028</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EHS64678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890384.1">
        <property type="nucleotide sequence ID" value="XM_003890335.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQ66"/>
    <dbReference type="STRING" id="418459.H6QQ66"/>
    <dbReference type="GeneID" id="13542052"/>
    <dbReference type="KEGG" id="pgr:PGTG_21028"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21028"/>
    <dbReference type="HOGENOM" id="CLU_2185256_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQ66"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053185">
        <property type="entry name" value="SET_domain_protein"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47332:SF6">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47332">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="111" mass="12842" checksum="328C8DF0F5E48D0D" modified="2012-04-18" version="1">MGAEHGKKSDTQIQRIEKLYQLSKESNLPLSEIEEFINLSEEETLPKFIAIAHLNAAKFYNSKKEMHKVREHAEKAKVMSEMSNEFKRLSHAVNDLETLLRDPEKHSSYGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRG6</accession>
    <name>H6QRG6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_21452</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EHS63255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889899.1">
        <property type="nucleotide sequence ID" value="XM_003889850.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRG6"/>
    <dbReference type="STRING" id="418459.H6QRG6"/>
    <dbReference type="GeneID" id="13540650"/>
    <dbReference type="KEGG" id="pgr:PGTG_21452"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21452"/>
    <dbReference type="eggNOG" id="KOG3624">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_590695_0_0_1"/>
    <dbReference type="InParanoid" id="H6QRG6"/>
    <dbReference type="OrthoDB" id="6475849at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000718">
        <property type="entry name" value="Peptidase_M13"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51885">
        <property type="entry name" value="NEPRILYSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS63255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="493" mass="54549" checksum="8A5C3CBA6B6FBDC8" modified="2012-04-18" version="1">MPSPDQQDPSLSGSPEQPPNEPVDEAEVELYGGGDDPEREALLAGHARILERKPTSVELRLAILAFFSLCAASIGFGLFAGELVKFHRAHPPDNGHPPVKIVTTTVFLSPTNAPSPPKNPNNVCASGPCVDAAAELRRTTQLSVDPCEDFAKFSTAGLRPAAREMISARVDSTIEHVLSGPTSPNPTLRKIQAFKSLCEREGSRPTYSEYSILLDEVTRLWMRKTGSEANRIGAVLAYLHAASLPALFKITVTSGRSRVVPQTISVEGLAEAFEAIYPANQVSQRVRSVQRFVATLSRLAADQEPGESDRLTVDQLQALSCPSPSLPCWIRWKDFYSALDPAQPLGLLTVHGLNYFAHIARAIAEQSEIGLESYFHWVVVLSMRRQNCQQETKLAFGPSLLQHLFLNTPDQLSNLHAHLRSARFILDSMSKRAIHSKLPRTIEGPPTESDQHTELSAIRSDSWVQTVYGLERIKTRREFSERRLRWNVLDTQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRN8</accession>
    <name>H6QRN8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21633</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178284">
        <property type="protein sequence ID" value="EHS63332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889835.1">
        <property type="nucleotide sequence ID" value="XM_003889786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRN8"/>
    <dbReference type="STRING" id="418459.H6QRN8"/>
    <dbReference type="GeneID" id="13540765"/>
    <dbReference type="KEGG" id="pgr:PGTG_21633"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21633"/>
    <dbReference type="HOGENOM" id="CLU_3107461_0_0_1"/>
    <dbReference type="InParanoid" id="H6QRN8"/>
    <dbReference type="OrthoDB" id="10250769at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="51" mass="5405" checksum="BA8BF78B93D9EE11" modified="2012-04-18" version="1">MGQSDGSNLSTNGVLDSITSITFRSESDTQMTVTSSSQGHLVIWNVNEGSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRR0</accession>
    <name>H6QRR0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_21533</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EHS63357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889795.1">
        <property type="nucleotide sequence ID" value="XM_003889746.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRR0"/>
    <dbReference type="STRING" id="418459.H6QRR0"/>
    <dbReference type="GeneID" id="13543040"/>
    <dbReference type="KEGG" id="pgr:PGTG_21533"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21533"/>
    <dbReference type="HOGENOM" id="CLU_012390_7_2_1"/>
    <dbReference type="InParanoid" id="H6QRR0"/>
    <dbReference type="OrthoDB" id="691997at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125">
        <property type="entry name" value="F21J9.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125:SF3">
        <property type="entry name" value="NO-APICAL-MERISTEM-ASSOCIATED CARBOXY-TERMINAL DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="2">
        <location>
            <begin position="135"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="181"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS63357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="310" mass="35085" checksum="88D81739EF27D29B" modified="2012-04-18" version="1">MARKRIQNVDPSLDDNDDEENNENTKKKKAPNYTEPEDFEVCRAWVQVSEDPLVGTNQDGNTFWSRVSTLYHEAVPDPIRPVNSLKKRWSNYLQPSINKFRGFVNLVESRNESGASAEDQLNRALRLYSQDQQSHFKYLRCYNLLAKSPKWHNYCRDNDKRGEKKRARSPSSEAPALTRLASTPAQSDPAAPSDPVSDFEGTSTDPKKLDRPIGNKKAKFLNQLAVKDLEWKEDVASAHKKLATESTRLNDIFANDSQSLKSMADNGETAAELTIMTQSLNGLDGEQQEYFKLKRAQILQSLRSGSTSNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSI0</accession>
    <name>H6QSI0_PUCGT</name>
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        <recommendedName>
            <fullName evidence="4">R3H domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21793</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178294">
        <property type="protein sequence ID" value="EHS63717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889539.1">
        <property type="nucleotide sequence ID" value="XM_003889490.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSI0"/>
    <dbReference type="STRING" id="418459.H6QSI0"/>
    <dbReference type="GeneID" id="13542802"/>
    <dbReference type="KEGG" id="pgr:PGTG_21793"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21793"/>
    <dbReference type="HOGENOM" id="CLU_2085961_0_0_1"/>
    <dbReference type="InParanoid" id="H6QSI0"/>
    <dbReference type="OrthoDB" id="278430at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.50">
        <property type="entry name" value="R3H-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036867">
        <property type="entry name" value="R3H_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82708">
        <property type="entry name" value="R3H domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="13073" checksum="B08AED442FB7011D" modified="2012-04-18" version="1">MEDSPPSTSSTSTTDQQQQQQQQQQPLNKNNNNLDPTLIEAINNGTIRDKQILLAAETEMGRFLSTDLQRQPLSSSLSNSLNSYQRMLVHRLGDSFGIKRTLESGQMYLERTATSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTA5</accession>
    <name>H6QTA5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22110</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178309">
        <property type="protein sequence ID" value="EHS64055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889272.1">
        <property type="nucleotide sequence ID" value="XM_003889223.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTA5"/>
    <dbReference type="GeneID" id="13541665"/>
    <dbReference type="KEGG" id="pgr:PGTG_22110"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22110"/>
    <dbReference type="HOGENOM" id="CLU_3107463_0_0_1"/>
    <dbReference type="InParanoid" id="H6QTA5"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="51" mass="5832" checksum="AF3CB74FA709F280" modified="2012-04-18" version="1">MPMQLSSLKHFLEVLDRLNVVGPGPPQSLILLHIFTYLRKFPPAAQHEHTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTJ0</accession>
    <name>H6QTJ0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22129</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178315">
        <property type="protein sequence ID" value="EHS64205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889164.1">
        <property type="nucleotide sequence ID" value="XM_003889115.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTJ0"/>
    <dbReference type="GeneID" id="13541221"/>
    <dbReference type="KEGG" id="pgr:PGTG_22129"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22129"/>
    <dbReference type="HOGENOM" id="CLU_2110176_0_0_1"/>
    <dbReference type="InParanoid" id="H6QTJ0"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="14"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="37"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="115" mass="11444" checksum="94008BD16263D541" modified="2012-04-18" version="1">MAGLVQQVATPVHRTAAQPAPQTAVPAARTAVLDARTAVPAAQTTAQTAPAARPAIPAAPAARSGKSADGAGSPDFRKTVLNILTSCYVSCSLASSQLSSLFSASSGRARLTVTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTN3</accession>
    <name>H6QTN3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22165</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178318">
        <property type="protein sequence ID" value="EHS64248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889127.1">
        <property type="nucleotide sequence ID" value="XM_003889078.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTN3"/>
    <dbReference type="STRING" id="418459.H6QTN3"/>
    <dbReference type="GeneID" id="13541346"/>
    <dbReference type="KEGG" id="pgr:PGTG_22165"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22165"/>
    <dbReference type="eggNOG" id="KOG0266">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1008794_0_0_1"/>
    <dbReference type="InParanoid" id="H6QTN3"/>
    <dbReference type="OrthoDB" id="10257301at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="276" mass="29896" checksum="32B8B72F0C449A78" modified="2012-04-18" version="1">MGSEPGLTDLPVITIQHDFPEVISDIKNEVCLAEDIWISCYRSSSSSSNTTSNTTESRRSIHGKVRVSSNVLDHPQQTQNNLNDLISLEGRDGVECRWADDASFSQLALIVTCEPLSIPPTTVSFPSQTLARIQPNGIECMDVAGEWLVTGGKNGQVRVDRWATQPADTLAHHDELLCRIGKGHVSDLTSCQFFPSGKVILTTSIDMSARIYSIDPVPSDDNEEEDSRTPTRKRMTMLNPRTFGPPHGRGVIGAGIIGKGREIVTGVGTGSCGYGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>H6QU04</accession>
    <name>H6QU04_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_22254</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178326">
        <property type="protein sequence ID" value="EHS64418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889017.1">
        <property type="nucleotide sequence ID" value="XM_003888968.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QU04"/>
    <dbReference type="STRING" id="418459.H6QU04"/>
    <dbReference type="GeneID" id="13540990"/>
    <dbReference type="KEGG" id="pgr:PGTG_22254"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22254"/>
    <dbReference type="HOGENOM" id="CLU_012390_7_0_1"/>
    <dbReference type="InParanoid" id="H6QU04"/>
    <dbReference type="OrthoDB" id="2506822at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023:SF4">
        <property type="entry name" value="GLYCINE-RICH PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="2">
        <location>
            <begin position="170"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="325"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="201"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="211"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="373"/>
            <end position="409"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS64418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="409" mass="46501" checksum="D2CB72D1C091E1C2" modified="2012-04-18" version="1">MDTPTRRSPRKKKGKPVPKKKPDAVATPKPPETSTTNNEDTPKPEEKKRGFPRWSVEEDKKLCVAWLNTSRDAIVGNGQKAATFWERIHEMHSELINEYNEEKKGAKGFKELPLRPVGAVECRWAHILKVLNKFSGCYSNVERRMKSGKTREDVLTEAKELYKTSSGSCFNLDHCWGILKDTPKWQATQQENDGRGKKAIQAQSNSQSTVAAPSSDMPSSTPAASSPTVVIDADEDDPENSRSVIGNVRLEGQKAAKRKRAEDNAIEKIISMQRDLVQISRERLSSMQASMRSSADEAVMSKDLSMLDEDSRTYYQKKKRAIIAREIQEEKEMEEKARKERDLKEKKEKEEKQRKEKEEKQAKEKAITAHNETEEEEDEDNAEDEAEDEAEPDVEDEAEATDDDVDEDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUF8</accession>
    <name>H6QUF8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22426</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178338">
        <property type="protein sequence ID" value="EHS64619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888861.1">
        <property type="nucleotide sequence ID" value="XM_003888812.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUF8"/>
    <dbReference type="GeneID" id="13542430"/>
    <dbReference type="KEGG" id="pgr:PGTG_22426"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22426"/>
    <dbReference type="HOGENOM" id="CLU_2050825_0_0_1"/>
    <dbReference type="InParanoid" id="H6QUF8"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="120" mass="13352" checksum="4BDFA57A83204F5A" modified="2012-04-18" version="1">MTDSHEATVAFDEKAGAKKMVVLTDDTFDSWRTSLINHLVLKNLEDLVLGGDPPAGVTDTIAQRKRIATTMIRMSLDKDNESRFVDRNKLRVLNLSFCLTTLSGIMSESPPKMQPPFGIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUI4</accession>
    <name>H6QUI4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178342">
        <property type="protein sequence ID" value="EHS64696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888831.1">
        <property type="nucleotide sequence ID" value="XM_003888782.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUI4"/>
    <dbReference type="GeneID" id="13542464"/>
    <dbReference type="KEGG" id="pgr:PGTG_22405"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22405"/>
    <dbReference type="HOGENOM" id="CLU_046890_0_0_1"/>
    <dbReference type="InParanoid" id="H6QUI4"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="313" mass="35064" checksum="A25DFBFC94EF1D62" modified="2012-04-18" version="1">MRGIEYGDKDIKTVMRQTNKWLLDLHKDSHVETVDFDILAVFIMQETDAKMHIRDLVATIIKTFAGSSTPQTNRQNQLLDAAFVVYETIYRYDQGVLLQPDFPKPFCIRHPSLLDVLKYSMKMEKKCKIIEEAKKMIILIDENGICVGDGLPPYPAPPKDSKHIAHDARALATLKEMVETPVCKLNLNQYPPLFVENPPSGPPQTPFSLNSKTKGDVRAPAKSLDASVSYQTYGFGLGGKKSAGVLDKKIACDGKSKSIKTEELQGHNDGWKEKKIKPELPDPLRDYSNTLDNQALAKLRNEIVEILTLSSHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>H6QVD3</accession>
    <name>H6QVD3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22709</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178397">
        <property type="protein sequence ID" value="EHS62862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888532.1">
        <property type="nucleotide sequence ID" value="XM_003888483.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QVD3"/>
    <dbReference type="GeneID" id="13540817"/>
    <dbReference type="KEGG" id="pgr:PGTG_22709"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22709"/>
    <dbReference type="HOGENOM" id="CLU_1441703_0_0_1"/>
    <dbReference type="InParanoid" id="H6QVD3"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="188" mass="20914" checksum="AE444A8907589C8C" modified="2012-04-18" version="1">MANQTWTGPEPPITHPDGTVEYVRPYDHAVRRELYGNRRSRRLRNRSPPITPPPPSLTLTRHIQTPANIFDTDEEADNEVMTHIAQELAPDTPEVTLQAAIDAIPTSLAEQLGLLDIAPERIELAIQLMNVPEEHQWPLTVIMLMRGDPNPEPAGATAAQSMPAPPELACHPRGRRNHTFAFPLALQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>H6QVH8</accession>
    <name>H6QVH8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_22748</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178424">
        <property type="protein sequence ID" value="EHS62993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888486.1">
        <property type="nucleotide sequence ID" value="XM_003888437.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QVH8"/>
    <dbReference type="STRING" id="418459.H6QVH8"/>
    <dbReference type="GeneID" id="13540974"/>
    <dbReference type="KEGG" id="pgr:PGTG_22748"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22748"/>
    <dbReference type="HOGENOM" id="CLU_012390_7_2_1"/>
    <dbReference type="InParanoid" id="H6QVH8"/>
    <dbReference type="OrthoDB" id="2503177at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125">
        <property type="entry name" value="F21J9.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125:SF3">
        <property type="entry name" value="NO-APICAL-MERISTEM-ASSOCIATED CARBOXY-TERMINAL DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="2">
        <location>
            <begin position="154"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS62993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="326" mass="36338" checksum="58CE0519BAC02BDB" modified="2012-04-18" version="1">MTRRPSCPVDLTTNDEDDNGDQNNDNGDNGDQNNDNDNANPENEGKKKAPNYTEPEDYELCRAWVQVSEDPAVGTNQDGGTFWQRISTVYHEAVPRPIRPVTSLKKRWSNNVQPPINKFRGIVHQVEALNQSGASTKDQLNRALRLYAADQLTHFKHLRCYNLLVKSPKWNSYCRDNDAKEAAKKKRARSPSSEAPPSNQLASTPAPSDPVTDFEGTGTDPSPMERPIGKKKAKTLIQIAAKEQVWKDEVASAHGKIASESKRLNDIFDNDSQSLKAIAQNSTTTSQLAIMNTNLEGLDDEQKEYFKLKRAAILKSLREEANSSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>H6QVI0</accession>
    <name>H6QVI0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP-dependent DNA helicase sgs1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22755</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178425">
        <property type="protein sequence ID" value="EHS62995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888484.1">
        <property type="nucleotide sequence ID" value="XM_003888435.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QVI0"/>
    <dbReference type="GeneID" id="13540975"/>
    <dbReference type="KEGG" id="pgr:PGTG_22755"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22755"/>
    <dbReference type="HOGENOM" id="CLU_011478_2_1_1"/>
    <dbReference type="InParanoid" id="H6QVI0"/>
    <dbReference type="OrthoDB" id="10261556at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS62995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="120" mass="13209" checksum="5F469E7437113A07" modified="2012-04-18" version="1">MEVPKKTQACNEDELKGLIERQGLKKYNQPCKPRQVETVASLIRGRNTFLLAATGFGKSRIPELYLDLLCKDRSGDIQGVVVVLNPLDALGDNQVAEKIQAGFTAINLTQLNFIVQGNPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQR0</accession>
    <name>E3JQR0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_00466</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307516.1">
        <property type="nucleotide sequence ID" value="XM_003307468.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQR0"/>
    <dbReference type="STRING" id="418459.E3JQR0"/>
    <dbReference type="GeneID" id="10527836"/>
    <dbReference type="KEGG" id="pgr:PGTG_00466"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00466"/>
    <dbReference type="eggNOG" id="ENOG502SDY1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003292_5_0_1"/>
    <dbReference type="InParanoid" id="E3JQR0"/>
    <dbReference type="OMA" id="SCTRIDA"/>
    <dbReference type="OrthoDB" id="3254696at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052925">
        <property type="entry name" value="Phage_Integrase-like_Recomb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34605:SF3">
        <property type="entry name" value="P CELL-TYPE AGGLUTINATION PROTEIN MAP4-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34605">
        <property type="entry name" value="PHAGE_INTEGRASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47823">
        <property type="entry name" value="lambda integrase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP74510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="361" mass="40763" checksum="A29C47272A1F6E41" modified="2011-01-11" version="1">MSLHKRNMIPFNKINEFLRNGTLVRSPSNVDLHILSGWQVSTLKSYNSAVIKYLKFSATAGKHTFVLPVSPEDIENFCLWAGRSLFSQDSSKISVCSIKKYLAGLRAWHVFHKEKFPDINKARIDLMLKASAKEDELVGKRAKKLPMMLWHMTHLWKELSHGSDFDKAVLDMAVVAFWGLARLSELSYDSELGTVNFTSSILMSDVVFDTENVGVASIAIRNAKTGAPGKPQLITLREQDHVLCPILALRRRLSSSLGTTTTLFGYMENGVRHHLTRRRAVTRVEQVLAEGGYAGLHGHSFRVGGASFRLALGMTTQDLCFLGRWKSDCYKLYIRPYDPDTKKKTLMLLSNLKLNWKRMGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRX4</accession>
    <name>E3JRX4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">A-factor receptor</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_01392</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the G-protein coupled receptor 4 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP74799.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319218.2">
        <property type="nucleotide sequence ID" value="XM_003319170.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JRX4"/>
    <dbReference type="FunCoup" id="E3JRX4">
        <property type="interactions" value="81"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JRX4"/>
    <dbReference type="GeneID" id="10546913"/>
    <dbReference type="KEGG" id="pgr:PGTG_01392"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01392"/>
    <dbReference type="eggNOG" id="ENOG502S44N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027592_5_1_1"/>
    <dbReference type="InParanoid" id="E3JRX4"/>
    <dbReference type="OrthoDB" id="2874149at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004932">
        <property type="term" value="F:mating-type factor pheromone receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14966">
        <property type="entry name" value="7tmD_STE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001499">
        <property type="entry name" value="GPCR_STE3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28097">
        <property type="entry name" value="PHEROMONE A FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28097:SF1">
        <property type="entry name" value="PHEROMONE A FACTOR RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02076">
        <property type="entry name" value="STE3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00899">
        <property type="entry name" value="GPCRSTE3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0297">G-protein coupled receptor</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0589">Pheromone response</keyword>
    <keyword evidence="8" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0807">Transducer</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="158"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="271"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP74799.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="379" mass="41879" checksum="8FAC712B2BF92646" modified="2012-04-18" version="2">MASGALINTYLSLSLLCGLLNIPPTILHISQGHSGPASFGLWATTLNILAFTNGVIWRNDALDRAPVLCDISSKISEVGPLGLLIANCCIIRYLALILTPNRTIEEPQQKRRRILLDYCLSLGFPSLIAAVSVIYQVGRYQINNLAGCSSASALVWPTFILSIVWPLVFCGISCCYSLYVLYCLIQRHRDIKKLVNCTGSTLNVSRFARMGALSVTYFCVATPCAVYGTLETIAATGPYVPWVSWSTVHNEDNKLSMVRQYPLYQYQLRDWLPIVAGLMVICFFSCGSESVSMYSKVGLACLDRLDAVKEKIFATRSPRRNHEPRSVFSDVMTSSSAEKLHTRKLINSGLFPSTTAPQTSLRPFQIHVSVVENSVPAEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3JSX8</accession>
    <name>E3JSX8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7872 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_01746</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75153.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319572.2">
        <property type="nucleotide sequence ID" value="XM_003319524.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JSX8"/>
    <dbReference type="GeneID" id="10547595"/>
    <dbReference type="KEGG" id="pgr:PGTG_01746"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01746"/>
    <dbReference type="HOGENOM" id="CLU_030195_1_0_1"/>
    <dbReference type="InParanoid" id="E3JSX8"/>
    <dbReference type="OrthoDB" id="2501761at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057194">
        <property type="entry name" value="DUF7872"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33339">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33339:SF1">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25278">
        <property type="entry name" value="DUF7872"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="97"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7872" evidence="2">
        <location>
            <begin position="154"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP75153.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="371" mass="40947" checksum="BC18B711663A504F" modified="2012-04-18" version="2">MGMDQHLQKYPGGLNLSLSDYADKVGATNFYCGIGNDCNPSQTCLGIKQQDWYALAAAHRFNTFVNQIYEATAYALGVVSDLSPTMVDDLIPDATNFWNFAAVYVGILTAWITGTPAALFGPGVGSVIWMVMMSTMFVSSAAVWVVANLYIPDPQKFTRWSEVIYRLQDFENHMQGALSNFTQSVIDSGISTQQGLYGISKDGNLFDDVQFRTESEIQSDLEQTLKLRSLSHILRIQNAFVTRGSQPCNGDGPNGALTEAGKLSYCDNEGIMMNIVLAGAKNREEPVYNAGMVAEKYGLSTELLTTTAWRCQQKYGVYRHPTVVNTTQPYDTSSDCLFNLPVCDLTDPTIRDNKHHMSVVEACRKLGKLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>E3JTJ0</accession>
    <name>E3JTJ0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Eukaryotic peptide chain release factor subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_01908</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the eukaryotic release factor 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75315.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319734.2">
        <property type="nucleotide sequence ID" value="XM_003319686.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JTJ0"/>
    <dbReference type="FunCoup" id="E3JTJ0">
        <property type="interactions" value="805"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JTJ0"/>
    <dbReference type="GeneID" id="10542781"/>
    <dbReference type="KEGG" id="pgr:PGTG_01908"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01908"/>
    <dbReference type="eggNOG" id="KOG0688">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035759_2_1_1"/>
    <dbReference type="InParanoid" id="E3JTJ0"/>
    <dbReference type="OrthoDB" id="10254527at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018444">
        <property type="term" value="C:translation release factor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016149">
        <property type="term" value="F:translation release factor activity, codon specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002184">
        <property type="term" value="P:cytoplasmic translational termination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.60:FF:000001">
        <property type="entry name" value="Eukaryotic peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.960.10:FF:000001">
        <property type="entry name" value="Eukaryotic peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000006">
        <property type="entry name" value="Peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.960.10">
        <property type="entry name" value="eRF1 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024049">
        <property type="entry name" value="eRF1_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004403">
        <property type="entry name" value="Peptide_chain-rel_eRF1/aRF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03676">
        <property type="entry name" value="aRF1_eRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10113">
        <property type="entry name" value="PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03463">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55481">
        <property type="entry name" value="N-terminal domain of eukaryotic peptide chain release factor subunit 1, ERF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="6">
        <location>
            <begin position="6"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="347"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP75315.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="468" mass="51553" checksum="A269398912E9EB1E" modified="2012-04-18" version="2">MSGQPQSEAEQNIQIWKIKKLVKSLEAARGAGTSMISLILPPKSQISQAANMLAQEYGTASNIKSRVNRLSVLAAITSTQQRLKLYNRIPPNGLIVYCGTILTDEGKEKKVNFDFEPFKPINTSLYLCDNKFHTEALSELLESDAKFGFIVMDGNGSLFGTLAGNTREVIHKFTVDLPKKHGRGGQSALRFARLREEKRHNYVRKVAEFAVQHFITNDKVNVQGIVMAGSADFKTELSQSDMFDPRLAAKVIKIVDVSYGGENGFNQAIELAAESLSQVKFIQEKKLIQKYFDEIALDTGKYCFGVEDTLRGLELGSVETLIVWENLDITRHTLRSSTGETIIIHTNAPPPTASNNTSAGQSGGNSGGTSLALAHLPEKEREKFIDKATGLEMEQAAEPQSLLEWLAEKYQEFGAALEFVTNRSTEGSQFVRGFGGIGGILRYKVDFGLIADAFEDGEEGEFMTDSDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3JTV6</accession>
    <name>E3JTV6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00784</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <dbReference type="AlphaFoldDB" id="E3JTV6"/>
    <dbReference type="GeneID" id="10543083"/>
    <dbReference type="KEGG" id="pgr:PGTG_00784"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00784"/>
    <dbReference type="HOGENOM" id="CLU_1156876_0_0_1"/>
    <dbReference type="InParanoid" id="E3JTV6"/>
    <dbReference type="OrthoDB" id="10685089at2759"/>
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    </evidence>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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            <dbReference type="EMBL" id="EFP75522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
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    </evidence>
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        <lineage>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="418459.E3JUI2"/>
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        <property type="entry name" value="CBM21_dom"/>
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        <property type="entry name" value="CBM21_dom_sf"/>
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        <property type="entry name" value="PP1_regulatory_subunit_3"/>
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        </source>
    </evidence>
    <sequence length="819" mass="87838" checksum="AA682ADA93874E27" modified="2012-04-18" version="2">MTSRMQISYSESQTALLSPPSSSASSDSIPQLTQSIHQLPPSSTGGTQTTPKNHHSLTINTNNCHQQPPADHHSNNRPSSNHTMSLTINTTATATHHNNNNNNNKMFSLETPPPSNSQSSTPSSAATSSTPTSTTPTHFIHQKSYSSSYSFPIVNFNSPTPPSTTADRSDYFDHNLPSPTRIHQQQQQQQQSPKPIRPVHQKAQSESVMRLELPLRFPSSSSLTSLDNIPMLRKKSGEVVRSSLKLSSNQGFASSSSSSICSSSRSSSYSNSRSAPSTPTQGPKAVHFDAHLEHVRHFLSQQRPIAVSRDGSPIETETEGEEEYPFPDLLNSDDHRHSDRSKLVIELPNMPLHPRLTLLNKVCLKSLELATDGKNLKGTVLVENLSFEKRVAVRFTFDSWQTVSEVTADYLSSCPPVPSPPKSTTTTSSSIKGGPSPAADLFGFTIKLQDVLARIHQREMEIAVRYLANGGEFWDNNSNANYHVKFKMLRHGGNNPHQASSNNQQQQQQQMGLSTSNGTTPTTTTTNGQPQNRKTRGDSSDPSGPWATTLRAELDRLVGDDLGLSSSSGIGGLPDGVIKKKKTHSSSFSSFGAAGDSKADFMGNATGNLGHFRAGSALSARYDFGLSLKQAAAVKHLQSSTPSNGMANGKIGVGNTHRFTPAIPLPISNVPVEWIGINSTNPHHRPSPSAASAGGFLNSFDLSDHRPPVHPLHYQPPESLTYHSTASNKPGLSKRWHSSAPAVHQPIVQIPLLPPSPPQEPSSSDAPTPTTDTSSSSSSNTTPTSSAASADPSSAAGFSAFLNSGIVTPQSPSLTDDSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWA8</accession>
    <name>E3JWA8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tetratricopeptide SHNi-TPR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_02774</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NASP family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320752.1">
        <property type="nucleotide sequence ID" value="XM_003320704.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JWA8"/>
    <dbReference type="FunCoup" id="E3JWA8">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JWA8"/>
    <dbReference type="GeneID" id="10534225"/>
    <dbReference type="KEGG" id="pgr:PGTG_02774"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02774"/>
    <dbReference type="eggNOG" id="KOG4563">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_604298_0_0_1"/>
    <dbReference type="InParanoid" id="E3JWA8"/>
    <dbReference type="OMA" id="IAECHYK"/>
    <dbReference type="OrthoDB" id="2505766at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034080">
        <property type="term" value="P:CENP-A containing chromatin assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006335">
        <property type="term" value="P:DNA replication-dependent chromatin assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051730">
        <property type="entry name" value="NASP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019544">
        <property type="entry name" value="Tetratricopeptide_SHNi-TPR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15081:SF1">
        <property type="entry name" value="NUCLEAR AUTOANTIGENIC SPERM PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15081">
        <property type="entry name" value="NUCLEAR AUTOANTIGENIC SPERM PROTEIN NASP -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10516">
        <property type="entry name" value="SHNi-TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 6" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="6">
        <location>
            <begin position="223"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Tetratricopeptide SHNi-TPR" evidence="8">
        <location>
            <begin position="224"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="104"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="306"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="405"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="123"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="159"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="412"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF10516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP76333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="453" mass="48095" checksum="8290F40F08DAD7B7" modified="2011-01-11" version="1">MTDSSSNQPSENPKAKEEPVAEAQNGEASDTDIDTIEAELAAGARAFAFKNYAIATECFSKACEKLTTHHPQNHSAIIDAHLSYGKALLRNAITLGSLSGGLNAHNTAATEPEDTPSEVAGNAPSSSKVGASAASASKPPQPALKGIKGNPQIHFSGDSSDEEGDEGEEGDEEIDQAEEPVTVEDELENAFQVLEFARQSILSQIDSSKDQPEQVEKLQNKLIDVYDLIAQVHQEGEQFEQAVESYKSSLEIKKSRTDITAGAIAETHLMIALALELIPDEGESMKQAIGHVDEAIELMRAQLAKLTQKSEGQTEGKGKGKSTGSSGEAEQDAGAADKLKLDREETQSLIAELEAKREELKTVPQAPAMSDADKALQVYLAAVNNNPSTTNGKAAINDLTNLVKKRARPVDSSSDPQPNTSSSSSAIPTITTTIPPPETPSKKLKTSDQTADL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3JYZ0</accession>
    <name>E3JYZ0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03221</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77265.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321684.2">
        <property type="nucleotide sequence ID" value="XM_003321636.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JYZ0"/>
    <dbReference type="GeneID" id="10537809"/>
    <dbReference type="KEGG" id="pgr:PGTG_03221"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03221"/>
    <dbReference type="HOGENOM" id="CLU_1971613_0_0_1"/>
    <dbReference type="InParanoid" id="E3JYZ0"/>
    <dbReference type="OrthoDB" id="10295185at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77265.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="127" mass="14326" checksum="96EA4ADDE39CC25A" modified="2012-04-18" version="2">MVYPSQQYPSLTSRYKQTRSDSLPDLEDYREMNNQSTRKAEVQVKPEVFGKSLPSQGNPKVHQRSIAISSSFPRSKSLLQAKSRGFDQVIQEYPKSSIAPGPLHRYPFFILAASSIQASQYSELIGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3K0R9</accession>
    <name>E3K0R9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_03850</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <dbReference type="eggNOG" id="KOG0191">
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        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A6"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN DISULFIDE-ISOMERASE A6"/>
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            <end position="72"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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    </feature>
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    </feature>
    <feature type="non-terminal residue" evidence="3">
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        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP78261.2"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04217"/>
    <dbReference type="HOGENOM" id="CLU_057374_0_0_1"/>
    <dbReference type="InParanoid" id="E3K1T6"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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    </feature>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78261.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="276" mass="28813" checksum="65AB2347C1F62A6E" modified="2012-04-18" version="2">MSIPPPSITIDPLGTSTPAATFTQTIPVAPSNPSSIPASTNAAQGTSPPPRPSKAVLSNLLSNPNAVETANQLRRPPSQLLETSNRVSKPSNRFPQDPDDTEPETSNPTKSPSNSSTTQTSTHKGHATKASPVHSFPVFGMPVGPHAQELISTKSTAENLNPDQELGHPPTINLPSQTSHPQNSNVPMQVGPPQNNDSTTQAPTIGLSIAPVSQAQRPSTHVLAPSSPPAGTATGPFNEARLETGLIPIVIESYDPEGQPFRSDGLLMISNIVPMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>E3K315</accession>
    <name>E3K315_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
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    <gene>
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    </gene>
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        <lineage>
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            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
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            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
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    </reference>
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            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
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            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDPK1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78872.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323291.2">
        <property type="nucleotide sequence ID" value="XM_003323243.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K315"/>
    <dbReference type="SMR" id="E3K315"/>
    <dbReference type="STRING" id="418459.E3K315"/>
    <dbReference type="GeneID" id="10536976"/>
    <dbReference type="KEGG" id="pgr:PGTG_04828"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04828"/>
    <dbReference type="eggNOG" id="KOG0592">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_370480_0_0_1"/>
    <dbReference type="InParanoid" id="E3K315"/>
    <dbReference type="OrthoDB" id="347657at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05581">
        <property type="entry name" value="STKc_PDK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000191">
        <property type="entry name" value="3-phosphoinositide-dependent protein kinase 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000534">
        <property type="entry name" value="Serine/threonine-protein kinase PKH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039046">
        <property type="entry name" value="PDPK1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF163">
        <property type="entry name" value="3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="177"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="130"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="331"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="504"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="621"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="719"/>
            <end position="772"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="8"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="60"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="87"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="505"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="621"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="725"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="207"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP78872.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="856" mass="94993" checksum="387E6C071369F5AA" modified="2012-04-18" version="2">MNNNQNIRPLTTLNNNNTRPTLHSLQPINNNSNNSSNSNTQEQPLSASSLSAPEHYSNPSSSVSRASSARSTSSVQAVPFRIPTRLTTTTTTTTTTTTTTTNTSNSPSNIDQLMITNINDQLIQPLQNLSIHQPPSSTSTQTTIIEQQKNLPRPELPRNPSTSSTNINSARKGIKDFIVGDILGEGSYSTVYHVKDKQVPDKEYALKVLDKRHIQKEKKTKYVAIERDTLNLLDHHPGCIRLYSTFQDECSLYYLLEYAPKGEILRSIKTLGSFSTDCARFYAAQILSAIEHMHSRGVIHRDIKPENILLDSEMRIKIADFGSAKILHPSSSTSTVTTTGKEPGSERSHSFVGTAEYVSPELLVQKVTSKSSDLWAFGCVLFQMITGVPPFRSRSEYLTFQKITHLEYEFPVGFPSDGQDLVSRLLVLEPEMRIGASMERGGIGEIKTHAFFDSVDWDRLWELLPPVLHPGLVVAAAPPPESLDQQPQPGPVYLPEFFQQDLLSPDPPLLPSPDPPFPPPEPHPPSAGLGLALLDSHEHLLTHPVDSDVDLVHQPESKASLGNALSKRKSWLLGAGRRKSKEDHFVYSSSGEMTWAEVYLPNELVIYSSVVNELWGIPGSSSSYSHSTTTSSGKPATASKMVESLRGKKKKKRQLILTDFPRLLCVKEDGKSSKVRVKFEVLFKPLPSPTTTTFTITSPVESRDGGEEGGVAHQRLAEGHCKTPSAASSSSHLTYSSSASHSTATRSHPQQPELASPRAPSVEPLHPPPHQHHLLPNQLLLFKKVEIENPRCFKIETVPIEYNNNSKNTHNAKQQQQQQQQKCCWFRFEDKSDAAQRWTDEINLAFHVANNKNHKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3K374</accession>
    <name>E3K374_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04887</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78931.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323350.2">
        <property type="nucleotide sequence ID" value="XM_003323302.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K374"/>
    <dbReference type="GeneID" id="10537119"/>
    <dbReference type="KEGG" id="pgr:PGTG_04887"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04887"/>
    <dbReference type="HOGENOM" id="CLU_101089_0_0_1"/>
    <dbReference type="InParanoid" id="E3K374"/>
    <dbReference type="OrthoDB" id="2157498at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029164">
        <property type="entry name" value="PIG-Y"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15159">
        <property type="entry name" value="PIG-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78931.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="188" mass="21527" checksum="B1ECFFCCDA8EA667" modified="2012-04-18" version="2">MPKRSRNALGIDPSQKEIGPALTPLIDEIDQDQGFNQAEIEKLIKATKSLASSLSDSDAKDIDQNLEETQSLNRSIVRNYHNQHQQQHQHTQGFSMMIWGLLIFFCSSFLWIIGIPLILFDLENFGDHLHISNQLDSPSNSSSSAWVKWILVKILDDHHYKFFIPLQITFGLVFVIINWGGLKIFRHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3C6</accession>
    <name>E3K3C6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">WW domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_04939</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <dbReference type="KEGG" id="pgr:PGTG_04939"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04939"/>
    <dbReference type="eggNOG" id="ENOG502RXNF">
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    <dbReference type="OrthoDB" id="2444812at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
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    </dbReference>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
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            <end position="91"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP78983.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="311" mass="33924" checksum="3D150401C08636C0" modified="2012-04-18" version="2">MESVQTPSKRTRSHSASSASDQASSHPTKAVKTESHHHPDQSPEQAGDSGPLHTSTTQLVVPLDDEHSQSQPEPSTGKSEKIQSKDSRDTSEAGLKAPPQEDPHPTASTSRESNAPASETGSQSELQTADPKPVVYSNGDWEAVYDTQTNAYYFVNSKTGETTWRNPLVPATEPAAVVVAAHPAPDLGGIDPELAHLDPHFARLIHGHASQTEPRFAARFNAKTGRFEGDPTRDPARVSEFARARTQAEAFFDVTGWEKTLEKHNGRVPGTSKETRAAALEQGKKLSKAQLNQFKQQKMEKKARNQRAWLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3K431</accession>
    <name>E3K431_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_04789</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78833.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003323204.2"/>
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    <dbReference type="AlphaFoldDB" id="E3K431"/>
    <dbReference type="GeneID" id="10536935"/>
    <dbReference type="KEGG" id="pgr:PGTG_04789"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04789"/>
    <dbReference type="HOGENOM" id="CLU_361358_0_0_1"/>
    <dbReference type="InParanoid" id="E3K431"/>
    <dbReference type="OrthoDB" id="2500434at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="115"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
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        </location>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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    </feature>
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        <location>
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        <location>
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            <begin position="805"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP78833.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="819" mass="91376" checksum="68ED50CA61CACBAF" modified="2012-04-18" version="2">MGSNLRPEEHSTSFYPIPPPRSNFHSDVIHSMTGVSRFRRFTRSHRKHHHHQENIETDSPSPTTPQTELISSQSSSLESRDIFHHTTPVKLDSAAGATGTSVAQLPGRHLIVPPWLAIVVWLAILFFVGLTVAGFSLSYQARKEFRMRVEEQRKAQEEAEQQTSECKIPITRKEFKSFTRKSIMVLNSNSPRGHHPQTNSVYSIDKSGELRPRNMFDRPPRDSILVAEITAPGQFSGDDPFYEFSRQMTREGLWDLAARKSKAISVVSLSSMMPKIGRRNSNRFTMMDLAEEELDEQNYHSSPLKENLARPSNSRAIRFLESSEGADLKIEDHMVQPNRFIPPVPKVPVQFVNERPDSGEKLSSFNDISFLSHKEDKQSSYSDQNSPLCTRIYPPQRTTSRRGREAEESTHLERANSQSTSVDVLSPPQRSTSLRKKGPRINHEQSGHHDHEDPETTDAIVRATTPFGDVSPDDAVAQYASTRAASVSPLPTLKHSKSSSMLGFILRSTTPTPHSLDGRESPAYTGHLESITEPLPMAGRSQESQDPPLARLSLSESVLDDYCKPFSVIFEDEDEDEDYFNPPRPHHTPYMPSNSTFWDNNPSGRSPDTENIPLPRRLPQAALARTSSHKSRLRSHTATSPELTSVAMTSTSSTPKTPSSSSFGARSRSASSGAQGSETSPHRSINHLSSSHDPPLTLKRNPSLSTNPYGRRRKQQDDSSLSRQLSGSTRRHTDRQDGPSGQQDDSYQQPSTSTRRHTERQDRSTKRHPPHSRTPRGSTQDPKSPDPSAPIRRAVSAQARIPLSGTMSSGGVRNSSFVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K552</accession>
    <name>E3K552_PUCGT</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05688</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79367.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323786.2">
        <property type="nucleotide sequence ID" value="XM_003323738.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K552"/>
    <dbReference type="GeneID" id="10531191"/>
    <dbReference type="KEGG" id="pgr:PGTG_05688"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05688"/>
    <dbReference type="HOGENOM" id="CLU_1644550_0_0_1"/>
    <dbReference type="InParanoid" id="E3K552"/>
    <dbReference type="OrthoDB" id="10301892at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79367.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="161" mass="17495" checksum="6EA562BBBA2FDC0C" modified="2012-04-18" version="2">MSTLERLDLYEKVQALATHPALKPNERGHIQALLNDIKADYENSDDKDMGRPEVTDIRRSPETPVGEEHTQTAVQSSALPGAQSSAQTTGTETHPASDSHPAKDVRTPNQSPTCAPRKGEFFILPNPIGAPRTTCPTFSQFSILSTRREGGVSSRKLGLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>E3K730</accession>
    <name>E3K730_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_05198</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>myo-inositol(out) + H(+)(out) = myo-inositol(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:60364"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EFP79973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324392.1">
        <property type="nucleotide sequence ID" value="XM_003324344.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K730"/>
    <dbReference type="GeneID" id="10533827"/>
    <dbReference type="KEGG" id="pgr:PGTG_05198"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05198"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_3_1"/>
    <dbReference type="InParanoid" id="E3K730"/>
    <dbReference type="OMA" id="WIFHYTM"/>
    <dbReference type="OrthoDB" id="2544694at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:001079">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF28">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00171">
        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00217">
        <property type="entry name" value="SUGAR_TRANSPORT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="53"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="171"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="300"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="333"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="364"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="404"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="435"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="11">
        <location>
            <begin position="14"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="487"/>
            <end position="512"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP79973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="512" mass="56711" checksum="2772468F8DC85E35" modified="2011-01-11" version="1">MWDDLHGSRLIFVISLLTALCFMLIGYDNGVIGGLTNAPAWNRTFNSPTGTELSTIVASYEIGCFAGAILAFVVGERLGRKRSIRLGAAWMVVGAVLQASAFGRPQLIVGRILSGIGMGCINSTAPVLQAEISPKATRGQYACAQLSTLNFGIFLSYWIDYGMTKHFSGSVSFRFPIALQLAMIIPIFMFTFVIPESPRWLFFHDRSSEGMEVLLRLEEADVDPEDPVLQAKFKEIEATVLYEKSIQVKGYRELFRNDGSSSRKRLLIACSVQFFQQLGGINGIIYYANFIFSSSLGFSANTASLVSGVLFTWFFLASFIPWFLIDTVGRRKLLISSVLFMGSIFAVLAGVVKQIEERPNDSFVYGVVAAIFCFLYLGAFTTGFQATVWVYPPEILPIRIRGKGTALATACNWIINFAVVQVFPPAVDKIGWKTYLIFMCFNFSFAPIMYIFYPETKGKSLEEIDLLFGDGVSADIRHQSISQLASPKPIESSLPMGYPESRKSTTLLDLEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>E3K8Q9</accession>
    <name>E3K8Q9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_06739</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325202.1">
        <property type="nucleotide sequence ID" value="XM_003325154.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K8Q9"/>
    <dbReference type="FunCoup" id="E3K8Q9">
        <property type="interactions" value="139"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K8Q9"/>
    <dbReference type="GeneID" id="10539803"/>
    <dbReference type="KEGG" id="pgr:PGTG_06739"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06739"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037639_1_0_1"/>
    <dbReference type="InParanoid" id="E3K8Q9"/>
    <dbReference type="OMA" id="LRFHHTM"/>
    <dbReference type="OrthoDB" id="167718at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043024">
        <property type="term" value="F:ribosomal small subunit binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033592">
        <property type="term" value="F:RNA strand annealing activity"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0034057">
        <property type="term" value="F:RNA strand-exchange activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097010">
        <property type="term" value="P:eukaryotic translation initiation factor 4F complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF11">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="124"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="28"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="258"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="99"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="260"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP80783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="317" mass="35261" checksum="C22119F013436EA8" modified="2011-01-11" version="1">MGEGKRLTKKQLKATAFRSKKKIKELIEEKSQALPEQDEDIDGEDDDNNTRKPSSSSSTSKKRKREKPSTASSSKPVVEDDQDEAQETSKTEIVNAAAFKRRKRKNAAKLKEKEEIQKRKSSKLILFIGNLPFDISVERLKSFFLEHCGEEPSVRLMTSKTSQSPGGTSVQPTTKGCGFIEFESSAALQKALRLHHTPLSAETDGPTNSRENVEKKARKINIELTAGGGGTSENRRKKILESHQRLAKQRDKILEKRIKSSTLDKSSNPANPPADPQFDVSIKLGSRPNPHKKHKSTPLNPTRFKPHFSGANAIKLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3K9X5</accession>
    <name>E3K9X5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">phosphoglycerate mutase (2,3-diphosphoglycerate-dependent)</fullName>
            <ecNumber evidence="2">5.4.2.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_07222</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.11" evidence="2"/>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP80970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325389.1">
        <property type="nucleotide sequence ID" value="XM_003325341.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K9X5"/>
    <dbReference type="STRING" id="418459.E3K9X5"/>
    <dbReference type="GeneID" id="10536209"/>
    <dbReference type="KEGG" id="pgr:PGTG_07222"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07222"/>
    <dbReference type="eggNOG" id="KOG0235">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033323_1_7_1"/>
    <dbReference type="InParanoid" id="E3K9X5"/>
    <dbReference type="OMA" id="NDCIIPQ"/>
    <dbReference type="OrthoDB" id="354304at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004619">
        <property type="term" value="F:phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061621">
        <property type="term" value="P:canonical glycolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07067">
        <property type="entry name" value="HP_PGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1240">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013078">
        <property type="entry name" value="His_Pase_superF_clade-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029033">
        <property type="entry name" value="His_PPase_superfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005952">
        <property type="entry name" value="Phosphogly_mut1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11931">
        <property type="entry name" value="PHOSPHOGLYCERATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00300">
        <property type="entry name" value="His_Phos_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53254">
        <property type="entry name" value="Phosphoglycerate mutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="6">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR613078-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP80970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="182" mass="20724" checksum="825BDE6929DB01F3" modified="2011-01-11" version="1">MGMFSWPEAKLTAKGRHQAELVGETLKSLGLKFDLAFTSGWIRAQESIEIVLEALDHKYIPLVKAPALNTRSYGSLGGRFKEDVRKEYGDQQVKYWDSTKFHNFPDNRPPEGETLKEGDERAKAYYNKQIKPEVLAGKTILILIHENPVLQGPREDYVLSFRILDMHMGQAPVEESQTCPFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAD0</accession>
    <name>E3KAD0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Double-strand break repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PGTG_06908</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Core component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The MRN complex is involved in the repair of DNA double-strand breaks (DSBs) via homologous recombination (HR), an error-free mechanism which primarily occurs during S and G2 phases. The complex (1) mediates the end resection of damaged DNA, which generates proper single-stranded DNA, a key initial steps in HR, and is (2) required for the recruitment of other repair factors and efficient activation of ATM and ATR upon DNA damage. Within the MRN complex, MRE11 possesses both single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity. MRE11 first endonucleolytically cleaves the 5' strand at DNA DSB ends to prevent non-homologous end joining (NHEJ) and licence HR. It then generates a single-stranded DNA gap via 3' to 5' exonucleolytic degradation, which is required for single-strand invasion and recombination.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2 16">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 16 18">Belongs to the MRE11/RAD32 family.</text>
    </comment>
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        <property type="protein sequence ID" value="EFP81287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003325658.2"/>
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    <dbReference type="AlphaFoldDB" id="E3KAD0"/>
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        <property type="interactions" value="661"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030870">
        <property type="term" value="C:Mre11 complex"/>
        <property type="evidence" value="ECO:0000318"/>
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    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
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        <property type="term" value="F:3'-5'-DNA exonuclease activity"/>
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    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
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        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="term" value="F:single-stranded DNA endodeoxyribonuclease activity"/>
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        <property type="term" value="P:double-strand break repair via homologous recombination"/>
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        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:meiotic DNA double-strand break formation"/>
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    </dbReference>
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        <property type="term" value="P:mitochondrial double-strand break repair via homologous recombination"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007095">
        <property type="term" value="P:mitotic G2 DNA damage checkpoint signaling"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00840">
        <property type="entry name" value="MPP_Mre11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.110.110:FF:000004">
        <property type="entry name" value="Double-strand break repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000011">
        <property type="entry name" value="Double-strand break repair protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.110">
        <property type="entry name" value="Mre11, capping domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003701">
        <property type="entry name" value="Mre11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038487">
        <property type="entry name" value="Mre11_capping_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007281">
        <property type="entry name" value="Mre11_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041796">
        <property type="entry name" value="Mre11_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00583">
        <property type="entry name" value="mre11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10139">
        <property type="entry name" value="DOUBLE-STRAND BREAK REPAIR PROTEIN MRE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10139:SF1">
        <property type="entry name" value="DOUBLE-STRAND BREAK REPAIR PROTEIN MRE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04152">
        <property type="entry name" value="Mre11_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000882">
        <property type="entry name" value="DSB_repair_MRE11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01347">
        <property type="entry name" value="Mre11_DNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="9 16 18" id="KW-0227">DNA damage</keyword>
    <keyword evidence="12 16 18" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8 16 18" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11 16 18" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="10 16 18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 16 18" id="KW-0464">Manganese</keyword>
    <keyword evidence="15 16 18" id="KW-0469">Meiosis</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 16 18" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14 16 18" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Mre11 DNA-binding" evidence="20">
        <location>
            <begin position="330"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="453"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="531"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="19">
        <location>
            <begin position="460"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="560"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="583"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="19">
        <location>
            <begin position="602"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="642"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="668"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="683"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="19">
        <location>
            <begin position="703"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="17">
        <location>
            <position position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
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        </source>
    </evidence>
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            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU003447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="SMART" id="SM01347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="EFP81287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="735" mass="81673" checksum="D795DB0A6AC4505F" modified="2011-01-11" version="1">MSDEEEQPLNGTLNLTNPCLPTPSITNKDDANTIKILVATDNHIGYAESDPIRGQDSINTFREILQLAVDHEVDMLLLAGDLFHENRPSRASLYSTIASLREYCLNDRPVRIELIGDSGIGIPHSFNFPPVNYEDRNLNVGLPVFSIHGNHDDPQGMGPEGALCALDVLSASGLINYFGRQELPGGAQRDEEALEEGLHIQPVLLQKGRTRLAMYGIGNIRDERFNYEMRSNRIRMSRPAEFKEQWFNLMLVHQNRVAHGPKNFVPEDGFGDDIDLVIWGHEHDCLITPQEIPGKGYFITQPGSSVATSLAKGESIKKHVGILEVQDKDFSLLPIPLKSVRPFIFDDIVLAEHEEEAKLKLDEKPKVVRYLKSLVEQLIKKATTDWNDEHDYDPDSPPMMLPLIRLRVEYSRADGLSAYDVGNPQRFGQDFLGKVANPKDLVQFYRRRKIGPRKPKNDIDLPEEEEIEDSQAKAPEKVQVATLVHQYLEAQNLGVLAENGMQRAVTLFVEKDDKDAIKEFYHNALEATQAHVKGSKADIGGSRQDRNPESEDEELLGELTKAKESYADTWEKNQKDELTTQKGKGKAVRKAKRNDDDSDESDFGKMSDVRYVDESDNGDPAGQSDSDISIRASVAPKKKAPAKAAPVKKAPAKKAAPAAKAPPKRAATKTAATSSRSRAAASQSQTTLDFSQQAPLRGASRRTAASQSNPQMVSDDSDDSIEVSTQRKTASRSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAN1</accession>
    <name>E3KAN1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07059</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81438.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325857.2">
        <property type="nucleotide sequence ID" value="XM_003325809.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KAN1"/>
    <dbReference type="GeneID" id="10537095"/>
    <dbReference type="KEGG" id="pgr:PGTG_07059"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07059"/>
    <dbReference type="HOGENOM" id="CLU_2741264_0_0_1"/>
    <dbReference type="InParanoid" id="E3KAN1"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81438.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="71" mass="7808" checksum="B37120A97AECB0E9" modified="2012-04-18" version="2">MSQFRHVYAGGEKTSGLRSRTMNTPARSRDIAQATNNPNSRHSVVTRLPRSPVSSGGRRNPRCGTKSAKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAX9</accession>
    <name>E3KAX9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_06834</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325632.1">
        <property type="nucleotide sequence ID" value="XM_003325584.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KAX9"/>
    <dbReference type="GeneID" id="10536698"/>
    <dbReference type="KEGG" id="pgr:PGTG_06834"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06834"/>
    <dbReference type="HOGENOM" id="CLU_049933_0_0_1"/>
    <dbReference type="InParanoid" id="E3KAX9"/>
    <dbReference type="OrthoDB" id="10280007at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP81213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="425" mass="48152" checksum="B12F3FF8CAFC958B" modified="2011-01-11" version="1">MAKESLPPFGGHRINPGINGKQINILGHNNSTPHYLNSEPEIPKYSGGRRKIVKIKPLDKNLCFDGSNMPIEEFISKYEEAAETVGASSQDLANQILPFIRGPDLQDEVEEMYGYENSNWKNFKEELMGRFSIKLTLEECAREELVDLVSYVANMGGISTPELFGQFILQVEHIAHHLIMNGYNSHMDEFSNLFWQSLSPNLEKAISDPLIWDGHLVLDENCHIAELPSYNIILEYIGMEFEKMDVLQEIPDQPEQKIDQTHQNYYKVNHMEEYSSEIQSPLTEKFPPVPSSTLGSCPLILENDPSLEQLEKEETKDFGSEIEILPAKIPPGTFSPCALIEENCPPVISVTLEFSPLISQNCEELEPLDNEEKENPGPEIATCEEIRPTEETLPVEEIPGSLGNLLRVNLEEKLGEKWMYHPQAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3KB54</accession>
    <name>E3KB54_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_07823</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325993.1">
        <property type="nucleotide sequence ID" value="XM_003325945.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KB54"/>
    <dbReference type="STRING" id="418459.E3KB54"/>
    <dbReference type="GeneID" id="10532955"/>
    <dbReference type="KEGG" id="pgr:PGTG_07823"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07823"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_407755_0_0_1"/>
    <dbReference type="InParanoid" id="E3KB54"/>
    <dbReference type="OMA" id="ISYTIHA"/>
    <dbReference type="OrthoDB" id="8117402at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050329">
        <property type="entry name" value="GLI_C2H2-zinc-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818:SF144">
        <property type="entry name" value="METALLOTHIONEIN EXPRESSION ACTIVATOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19818">
        <property type="entry name" value="ZINC FINGER PROTEIN ZIC AND GLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="582"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="7">
        <location>
            <begin position="612"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="459"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="519"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="26"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="489"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP81574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="674" mass="72308" checksum="DA112221D53BDDE9" modified="2011-01-11" version="1">MSTNSVISFSTTYPMSDASKSDSYRNDSSPPTSLISLESTDPFENSLSADTFLESESGSQGLSPAGSIHSSLYPDNLVSFQEYLMLDLFAYGLPTTEPDVAAKPTQMLPYPSITPLITLGTGEEQAQTLDETHQADVQEALNGLSQTAPDTYFHQAQAIKDWSGACSTALSTESAPSPNSSLPQEAHYMSTATCMDYPSSQTPASSSNDWSTKNKLNLTVNTNMTAFKAADQLSPNCQNPLTPYSESSASYISASAPSLYFEPQHSGHLSPLVPSPPSIVNWLHASLDAPQSNPGVSYCEGLPTPSSATFSAVPTPTGISFPSDYPHPLGKENSAMGINPAQTKFSLQLPVQSSGLLSPTNMDLTSGLGYQPVSASAPQVPHRQYQSNVYQLVNSPASYSSPLYSPTDYFTGLPQAPPNNYSSKFDHASTFGHMVSEPGFCAPLEKGPCSVPLALASPEDLDRSEQGKRALSQHTDSEDEHRPSKRRCSATSCDSANENVNPYQASGGVSSLLKRAPLVGSRLKPGPKSKSTLVQHPPPGANAIGLNPSGEACGIPKHVLKGLYDEISSPSKSGDHSSSKRFVCKLDDCRRSFARKTAIESHLQTHLEDKPFVCSTIDCNAAFVRQHDLRRHEQIHLKNKAHNCICGKGFARGDALMRHRQRGICAGSLVPRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBM5</accession>
    <name>E3KBM5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_07759</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81510.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325929.2">
        <property type="nucleotide sequence ID" value="XM_003325881.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KBM5"/>
    <dbReference type="GeneID" id="10537248"/>
    <dbReference type="KEGG" id="pgr:PGTG_07759"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07759"/>
    <dbReference type="HOGENOM" id="CLU_1619867_0_0_1"/>
    <dbReference type="InParanoid" id="E3KBM5"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP81510.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="164" mass="18236" checksum="08CA7E9056AB9BDB" modified="2012-04-18" version="2">MRQSFRKLYHHDQPISSVTCPRPPLYIGQQNGASCRVDQRFNTGGYASELYHIKLLTGSPGSLGAKSWCSEVALVKTYATGLAVVNEFEGVTLQAWPFDRGLGRKDLRTSSDDPSAAGKNRPSHTSLQFHYFLRLCTAWSSMCGTSLGVHRFRVKPSPFPKERA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBT1</accession>
    <name>E3KBT1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA repair metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_07975</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326145.1">
        <property type="nucleotide sequence ID" value="XM_003326097.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KBT1"/>
    <dbReference type="STRING" id="418459.E3KBT1"/>
    <dbReference type="GeneID" id="10533434"/>
    <dbReference type="KEGG" id="pgr:PGTG_07975"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07975"/>
    <dbReference type="eggNOG" id="KOG1361">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014428_0_0_1"/>
    <dbReference type="InParanoid" id="E3KBT1"/>
    <dbReference type="OrthoDB" id="5561659at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070419">
        <property type="term" value="C:nonhomologous end joining complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240">
        <property type="entry name" value="DNA CROSS-LINK REPAIR PROTEIN PSO2/SNM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240:SF8">
        <property type="entry name" value="PROTEIN ARTEMIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="188"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="523"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="762"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="622"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="646"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="657"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="680"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="792"/>
            <end position="801"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP81726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="848" mass="96101" checksum="34BFFEAEB8C89793" modified="2011-01-11" version="1">MSTFDGFIREFDGLVRVDNFQQSGWLPTGCRASLVYLLSHAHSDHMVGLETFAGATIWCSEVTKAIVLNIRPVQARVNEANGIGNGKMDQTYRNLLPKDSSEKPLLKTMKPYDPEEIPVINSKGGICRVTLIPANHCPGSAMFLIQMWNRNVLYTGDIRAEPWWVESLTKEPQLAPFVVPPQGINHRLVKPPQDKHSPKTIPSSEEETTRPFLQLNNIYLDTSSLLTKLDVLTKDEAIKLTIQMMSAYPKDTNFFINSWTWGYEELLQRIAVHFKTSIHVDDYKYSIYKLSDFRKQFPLLLARLTLDPDETRFHACERTDRCQKVLDKSREWQQSQSLDSGIKIGSTRSKEPLVVCVNPSEITTQKWNLYKAALDVKLATAARYTSENPQTEHDLWPQLLFCPLTRHSSFKEILNFISAFRPQCIFPNTTSADTGFVEFYAIPKLFGSLLSSDAAQKIQEQAIQFVKDYEKRHSQVRKKARPSVDLFDWKGIHFQKLEEVFQKFEELEFQSAKVQSMWKQSFPTINPNEKDEAAKFLGGPPAHENSNRKDSPEIIEIQSSDEDDSSDSETVPEPSETDQETQRNRSSEIYESDPLESSEMAESIREETPNEATRQLEEINWTPEQTSPTPEQVNHTLGDADCAPEEASRTPEEAKSPQHSSSDIPVTPSNPTPISRIPIASTSTNEGTSPFQDLHSSPAQPVSANSPESRPRSNGLDLSCERQTPSAWAAILQNIERQRYSEARALARHLRKYEQALDQLEDHDHSDGCHQSKKRKKAIDVGDTSDPENQSKRSSSIIIIDDSPKHIPPKDPLLVWIGVRDCIVTLTQLRAQTFHARQKGVEAIMRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCW4</accession>
    <name>E3KCW4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_07647</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82250.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326669.2">
        <property type="nucleotide sequence ID" value="XM_003326621.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCW4"/>
    <dbReference type="GeneID" id="10529623"/>
    <dbReference type="KEGG" id="pgr:PGTG_07647"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07647"/>
    <dbReference type="eggNOG" id="ENOG502RSDK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020819_0_0_1"/>
    <dbReference type="InParanoid" id="E3KCW4"/>
    <dbReference type="OrthoDB" id="2495627at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052980">
        <property type="entry name" value="Crinkler_effector"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33129:SF1">
        <property type="entry name" value="ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33129">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP82250.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="682" mass="76492" checksum="2A9E4DEAA3AB8459" modified="2012-04-18" version="2">MLPPQIWLSFKNKLVTVQTKELVYVINLAQAAKTEFPSSLRDFDTPDITLHTSEDAEALTSDSALAEIAPELSLCAASKTPLIVKVKLDVAPQTRLIAKGIPAIIWLSFKGNLVSVETKGLVFVNQLAKAARDEFPSSLGGFDTPDITLHTTEDAVALTSDSALAEIAAELSPCAVSKTPLIVKASKFIPDLHLHVHAEIIRFWKALPEATLVTNGEVQYLELKEGYVLGDKKLGSRFLVRPVYKELYDYIVDFNYSKVVVTGTPGIGKTIFSAYYMWIAACNQKTVVWEPCPSRKGGRATFLMTSGGVQRVNIDSHEVGEILVNDETVYIVDGQPPTKCEAQTLVVTSPLGENYVQYLKTSGTKLLYMPPWTYEELRNCKTIIYPDDQILPTALMDRLFEWYGGVPRYVVDLASVEFEKHGNEDEVVRDLVRNLTQAVYKGQVTDIKKAHDSGTREGEYSHRVLHICSHPSGRVNEFCVRFASLRVAQAVIERIEEELRTDLQNFLRTCSDSSGLRGLLFEPYTFKMLREGGRFPVRRLCKQDTPNEQDVSVEFAATERRTFRSYEEVDLRTNVFWQPVSKQLPSIDSLLGPANFFQATVSKTHPIKHQGLSKALELVDESECRPKLYFVVPSEIYPTYKSQPYHTKCGKVFMGNLGGVGKVEQWVLMIPMGGEWPVREEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDF5</accession>
    <name>E3KDF5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_08347</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82391.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326810.2">
        <property type="nucleotide sequence ID" value="XM_003326762.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDF5"/>
    <dbReference type="FunCoup" id="E3KDF5">
        <property type="interactions" value="615"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KDF5"/>
    <dbReference type="GeneID" id="10529768"/>
    <dbReference type="KEGG" id="pgr:PGTG_08347"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08347"/>
    <dbReference type="eggNOG" id="KOG2171">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003794_1_1_1"/>
    <dbReference type="InParanoid" id="E3KDF5"/>
    <dbReference type="OrthoDB" id="7862313at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057672">
        <property type="entry name" value="TPR_IPO4/5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25780">
        <property type="entry name" value="TPR_IPO5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00913">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50166">
        <property type="entry name" value="IMPORTIN_B_NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="9">
        <location>
            <begin position="28"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="8">
        <location>
            <begin position="391"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="8">
        <location>
            <begin position="899"/>
            <end position="937"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP82391.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1083" mass="118452" checksum="226E6F0E790C971D" modified="2012-04-18" version="2">MDPQFVQQLQSCLEQIATPSGTDGIKAATQALNEQFYRSPLAIPGLFEILTTCGTPAVRQLSAVELRKRVSAGKRKHWKKLESSMRDAIKARLLEIVVSEPVPITRHAIARVISEVAEYELPEKAWPQLLGFLIKATDSPVAHEREVAIFTLSSLMDTVVDSYAENLPQIYALFAKTLQDPESLEVRVTTVQALGRVAEYIEVDEEASIASFQAMIPQMLVVIGQTLEAGDENAAKKGFDTLETLLIIEVPLINAHFTQVVEFNATIGNNKSLDESQRIMALNCLLWTIKFKKSKIASMDLIKPIVDSLITIGAEDEPEDPEDDSVARTAFRCLDALSTSLSPQAVFPALYSRIQECFRSTDPTLRKAAVMALGVTVEGCSLFIQPHIEQLWPFIDTGLEDSDPRVRRAACTALSCICEMLVDECASRHQILVPRVSALLNDPACQRNAMTALDGLLEVLDDQTIGLYLHPLMERLVPMIDSAPPKLKGTVVGAIGSAAYAAKGAFEPYFDVCMQRITPFLSLKGEGDEQELRGVAQDTVGTLASAVGKEKFRPFLDGCLNIAFEAIELNSPSLRECSMIFFGTLAKVYESEFVAYLPRVMPAVFASLGQSEEDDGSVLPSEMIKGFKAADDDEDEAEEDSAFVDVEDVDLDDDDLMKTTTAVAVEKSVAADAVSELFEYTKTSFLPYLENSIKSLTPLLTHFYPTTRKAAATTLLSFITIAYEITDPPKFEPGMAHINMPNDVRKLVDLVIPEIMSVWRGEDECDVVSDLCSSLSSVISTVGAGVVAPTYLDETCTLILTILERKSTAQLDSDFEEASATGDLSEVESNLIGCAADLVGTFATVLGADFAQAFNQFLPCVSKYYDPCYSPTDRNNAIGSLAEVINGLGSAVGPFTEQLLPLGLKATKDEDVEVRSNAAFFLGSLAYWTTVDISSQYMSILECLQPLFTVPDDSSREKSERAKDNAAGAVARLILKNKAALPLDQLLPVFFEALPLKQDFAESSKCFEALFELIQQSHPLVQTHFDHILAVFAHVLQNSVPAVPEEKAMIPAETREKLVALLRQLNSQVPDQLAARGLTGYIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDU0</accession>
    <name>E3KDU0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_08481</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82525.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326944.2">
        <property type="nucleotide sequence ID" value="XM_003326896.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDU0"/>
    <dbReference type="GeneID" id="10529904"/>
    <dbReference type="KEGG" id="pgr:PGTG_08481"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08481"/>
    <dbReference type="HOGENOM" id="CLU_1759715_0_0_1"/>
    <dbReference type="InParanoid" id="E3KDU0"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP82525.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="148" mass="16373" checksum="52D8DEAA819C852B" modified="2012-04-18" version="2">MDGYEECDWDTLRASMIELWGDKYEIWLPPLWEHLTEAAGMETKLEELAKEIETSDGLHGASKRLYGTSNGLYGTSEGSYRASNGLYGTSDGSYGTSDGLHGASNILHKTSNRSYRASDGLYGNSKGLYGNRKGLYRARNKSYGSHHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3KEE4</accession>
    <name>E3KEE4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">FunK1A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08873</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82677.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327096.2">
        <property type="nucleotide sequence ID" value="XM_003327048.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KEE4"/>
    <dbReference type="GeneID" id="10530060"/>
    <dbReference type="KEGG" id="pgr:PGTG_08873"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08873"/>
    <dbReference type="eggNOG" id="ENOG502S5WB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005513_5_2_1"/>
    <dbReference type="InParanoid" id="E3KEE4"/>
    <dbReference type="OrthoDB" id="5592585at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040976">
        <property type="entry name" value="Pkinase_fungal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38248:SF2">
        <property type="entry name" value="FUNK1 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38248">
        <property type="entry name" value="FUNK1 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17667">
        <property type="entry name" value="Pkinase_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fungal-type protein kinase" evidence="1">
        <location>
            <begin position="179"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82677.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="633" mass="72452" checksum="5DEC1EDF2DA4A816" modified="2012-04-18" version="2">MLSLHHDGCFEPFKPLIAVTLADKAKSNQEMLKFIEEEFGSPPTPLPFIPTPPRTGTTSSLTNSSDLLRHFMPRLRQELQGLMYNNVPGLVERFIQQHTFDPALRLEPDESFIDEKYEETLKDTTEGSMLDWFTSFFDQVVKQSPQIRNSRTWKSQPTIPLKGLEAIRKLDGAIMSVNLENENRIEDVMIPLELKMNKSDASKAAMSLAKYVCHLFKAQPTRIFVIGFALCGTWMQLWQFDRSGAIGSEFFDIKANKKNLKKFFTLISWSLTCDKRLLGFDPTFIVDESPVIRIQTQAGEFVIDEPENPIFRASGICGRGTTCWKAHLAGDERQKFLIKDSWQPDHRREEGLMLRDVTQKNVHHVARYYHHEDVHVSGQRVDIESHVRGGTNFQSGQTIQINEMPDDAQVLNQFINRVHRRLVLKDVGKPISEANSPVELLEALEGCIKGHQALLNAGYLHRDISINNLMIDNQTADSDRKAFLIDLDVAIPYDVSNKDYTRTGTKVFLSISLLQKCNPHDFADDLESFFWVMIWICIHFPRKERKQTSVTSWNEQIPENLGSIKKDLLSEPSRLTNDFTARYKQSQPLIQCVHDFAKIMQDPAVRKKNSAHLYGEILNIFQQARGKLSQSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KEI0</accession>
    <name>E3KEI0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_08909</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82713.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327132.2">
        <property type="nucleotide sequence ID" value="XM_003327084.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KEI0"/>
    <dbReference type="GeneID" id="10530096"/>
    <dbReference type="KEGG" id="pgr:PGTG_08909"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08909"/>
    <dbReference type="HOGENOM" id="CLU_685383_0_0_1"/>
    <dbReference type="InParanoid" id="E3KEI0"/>
    <dbReference type="OrthoDB" id="10277996at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172570" evidence="2">
        <location>
            <begin position="27"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP82713.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="402" mass="45354" checksum="AFE8CEAEE114CA8A" modified="2012-04-18" version="2">MATSHWSHSFIFLLIFYLCLWTNVDGQEAKPGILDGGMTFAHKTQAEKSAREGCDVPMAPMKSSLMIETPKAEPVPSAKENPVSEKTNRSREPEEIHRNPAQLDTSENRKRRPRRTGFLAWTRHHLNRLWNWLCRLFNKSTSKDKRTEKWAPQADHEMLQNQKSDTLENIHENGPAPPDGANLENASAGHAAQVSTGEAHSEGDGSETVTPGPGKVKPDPGKNQPGPQKTPPESDPPKVEHHVEHVKNDPEKAKKIGISLNAERLALTKTVMDLLFEAIRHGIDSKTAILSSIDPIVREMGFIKKRMVPKGCARLCKKIEENIGRQKALIREIIGTDHHRSWAQLMQDTNKSAQFEDAFVSTFRECDGFIKSLQDWKYHNESPKRFLLHIEEYTSKLRAKAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>E3KEX7</accession>
    <name>E3KEX7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KEX7"/>
    <dbReference type="GeneID" id="10544734"/>
    <dbReference type="KEGG" id="pgr:PGTG_09990"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09990"/>
    <dbReference type="HOGENOM" id="CLU_2759015_0_0_1"/>
    <dbReference type="InParanoid" id="E3KEX7"/>
    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="70" mass="7697" checksum="A092A1161A8B5893" modified="2011-01-11" version="1">MEKRLLPRENIGLWRLMPSLPQVTSVKEDDRGDGGGSVNGTPHQLSYSLPVYSSYLSEYTSAATPGWPAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3KFB7</accession>
    <name>E3KFB7_PUCGT</name>
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        <recommendedName>
            <fullName evidence="7">CWH43-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
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    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    <dbReference type="FunCoup" id="E3KFB7">
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    </dbReference>
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    <dbReference type="GeneID" id="10544655"/>
    <dbReference type="KEGG" id="pgr:PGTG_09912"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09912"/>
    <dbReference type="eggNOG" id="ENOG502RZQS">
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    </dbReference>
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    <dbReference type="InParanoid" id="E3KFB7"/>
    <dbReference type="OrthoDB" id="10032492at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
    <dbReference type="GO" id="GO:0012505">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019402">
        <property type="entry name" value="CWH43_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050911">
        <property type="entry name" value="DRAM/TMEM150_Autophagy_Mod"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21324:SF2">
        <property type="entry name" value="EG:22E5.9 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21324">
        <property type="entry name" value="FASTING-INDUCIBLE INTEGRAL MEMBRANE PROTEIN TM6P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10277">
        <property type="entry name" value="Frag1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="9"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="153"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
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            <end position="232"/>
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    <feature type="domain" description="CWH43-like N-terminal" evidence="7">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
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            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="242"/>
            <end position="256"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP82944.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="293" mass="32988" checksum="F47BF039CC4A6182" modified="2012-04-18" version="2">MKLTEYGAYVWLPIITALGWFTTLAGLLSLRLFSAADIGKHRPDEASVLFISDVGAAHMIWFTPGCTLVALLYIATLLVERWLRHRERIPRVISKRHRYWGYSTAGHGILSGLSLMMLSFLSKFEHPTVHWRFTFMFAICLMISAYSQVIEVFCLKKEYPEQAHLRRNAMIKLWIVSIATVLAVVFGVLFLSCGGLVRSSRDNLLLSGAAVCEWAVSFLLTFYFLTFVLDFYPAAKTSSRLEPNKCHSQGKTDSRSAPKMVTPGVFDLKIDLEKSDRPDPLAIDPPHAQVAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3KFD3</accession>
    <name>E3KFD3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
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    <gene>
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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            <taxon>Puccinia</taxon>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178284">
        <property type="protein sequence ID" value="EFP82960.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="KEGG" id="pgr:PGTG_09928"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09928"/>
    <dbReference type="HOGENOM" id="CLU_358292_0_0_1"/>
    <dbReference type="InParanoid" id="E3KFD3"/>
    <dbReference type="OrthoDB" id="165382at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015693">
        <property type="term" value="P:magnesium ion transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.20">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="EmrE-like"/>
    </dbReference>
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        <property type="entry name" value="Mg_trans_NIPA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570:SF65">
        <property type="entry name" value="MAGNESIUM TRANSPORTER NIPA9-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05653">
        <property type="entry name" value="Mg_trans_NIPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="498"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="589"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="616"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="644"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="682"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="708"/>
            <end position="726"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="746"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="68"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="462"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="525"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="113"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="469"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="525"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="561"/>
            <end position="575"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP82960.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="782" mass="87306" checksum="A41AA2FBA79BBE35" modified="2012-04-18" version="2">MQNRWNLAVVINVALFHYRFLSTSEIQGLDLLANLACHAEDTKAGLNENHWTSLTQQGRIALERAQDKTKRVSDVHMFPSSSPDKASETVPSVEETNEDEFSQLDSKRRKLDPGVSVGSPVSGQSGWPSQRCHLSDSNRLTNAHVRESLKVEEQDSQFLVIRDVVFDTSTQDRSRDTNSESQPEKDELLPTRKVRQVIVDSKFWAWISITWSLLEHELSDQMKRHVESRHGKLIHHLPLDTALGADEEQTIEGFKYDLHLRKRLADLIVFLWAVNHQVLSILGTKKRFMIPHSSSDSCGKTILLQEDLDISRLAVMILGCYYKNQNIEKWSFLFKVDERFLIYLLNKSTELFHRDSLKAKKSKSFGSFKTYQIVPWKTQMKPELKLLNLELGHRNIADFKSWGSSCCTKNAADVAQAFSKAPYQAKAKVKNDPQSFESTSIRVQWTTAEQSVSLHHTQIYNDIHPRNPISDSRSTARTDASTLHTQTMPLNSDPSTSTLIGVGISVAGNLMISVALNVQKLAHQRLEPSEPRPAKKPSEATPLMIDEPQELHAPPTDLESPRSVSPASSSSSRAQSYQPNDLAYLSSPLWWLGFVIMSTGELGNFVSYGFAPASVVAPLGTVALVGNCVAAPVLLGERFKKRDWLGIGLVIIGTITIVLSSPRTSEALSPDQLARAIRQLGFILYAALCLSAILLLICLSSTQWANRFIGIDVGLCAISGGFTVLSTKAFSSLLNVLFLDCFHYSITWIMLAVMLVTAVLQIVFLNRALQRFDSKASLHQLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3KFW6</accession>
    <name>E3KFW6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09204</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EFP83251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327670.1">
        <property type="nucleotide sequence ID" value="XM_003327622.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KFW6"/>
    <dbReference type="GeneID" id="10544965"/>
    <dbReference type="KEGG" id="pgr:PGTG_09204"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09204"/>
    <dbReference type="HOGENOM" id="CLU_2122233_0_0_1"/>
    <dbReference type="InParanoid" id="E3KFW6"/>
    <dbReference type="OrthoDB" id="10268158at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173532" evidence="1">
        <location>
            <begin position="21"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="114" mass="12886" checksum="55CAEB7B74C77B5C" modified="2011-01-11" version="1">MARIWVFWGGLWLVCGLILASPNMGRQAGEMEVECARCKDTAFSSFNPGACNEPLVCSQHRVATGGRCTFWEEERFRIRCTHCAYTTYEHPNLPLHQHSVRCTRSCPSHGTPAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGB2</accession>
    <name>E3KGB2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dedicator of cytokinesis</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_08523</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DOCK family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83337.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327756.2">
        <property type="nucleotide sequence ID" value="XM_003327708.2"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KGB2"/>
    <dbReference type="GeneID" id="10545046"/>
    <dbReference type="KEGG" id="pgr:PGTG_08523"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08523"/>
    <dbReference type="eggNOG" id="KOG1998">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000595_3_0_1"/>
    <dbReference type="InParanoid" id="E3KGB2"/>
    <dbReference type="OrthoDB" id="18896at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08679">
        <property type="entry name" value="C2_DOCK180_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11684">
        <property type="entry name" value="DHR2_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.350">
        <property type="entry name" value="Dedicator of cytokinesis N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027007">
        <property type="entry name" value="C2_DOCK-type_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026791">
        <property type="entry name" value="DOCK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043161">
        <property type="entry name" value="DOCK_C_lobe_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043162">
        <property type="entry name" value="DOCK_C_lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032376">
        <property type="entry name" value="DOCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042455">
        <property type="entry name" value="DOCK_N_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027357">
        <property type="entry name" value="DOCKER_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046770">
        <property type="entry name" value="DOCKER_Lobe_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046773">
        <property type="entry name" value="DOCKER_Lobe_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056372">
        <property type="entry name" value="TPR_DOCK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653">
        <property type="entry name" value="DEDICATOR OF CYTOKINESIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45653:SF10">
        <property type="entry name" value="MYOBLAST CITY, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20422">
        <property type="entry name" value="DHR-2_Lobe_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20421">
        <property type="entry name" value="DHR-2_Lobe_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14429">
        <property type="entry name" value="DOCK-C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16172">
        <property type="entry name" value="DOCK_N"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23554">
        <property type="entry name" value="TPR_DOCK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51650">
        <property type="entry name" value="C2_DOCK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51651">
        <property type="entry name" value="DOCKER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2 DOCK-type" evidence="6">
        <location>
            <begin position="851"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="domain" description="DOCKER" evidence="7">
        <location>
            <begin position="1866"/>
            <end position="2281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="66"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="148"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="213"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="777"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1448"/>
            <end position="1474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1572"/>
            <end position="1597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1665"/>
            <end position="1693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2405"/>
            <end position="2431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="2490"/>
            <end position="2581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="148"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="216"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="250"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="778"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1448"/>
            <end position="1466"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1572"/>
            <end position="1592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1665"/>
            <end position="1682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="2405"/>
            <end position="2427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="2492"/>
            <end position="2508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="2509"/>
            <end position="2521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="2538"/>
            <end position="2557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP83337.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="2581" mass="288044" checksum="FE416F36ED948A69" modified="2012-04-18" version="2">MSQDQQQQLLLPLPLPPPLNNNYPNQEHSEQQQPLSFSGRWIPLPKILYGTTIYPFNPIQIDPRKVDIKGKGKQPTEEPSQDNLPNLVPANPNQVPLDVGDECYVFEQYLPASHPIDPSSIWYRGYVVTISHQPSSLSNLPVSQLNRADSQPFESTTNNTDHFHSPAHLPDEPQVFLGIFPASHLQIREHLDDSSHRSDRSLAQIRAASAAHLHSIQGQSNNSNNAHLLRSFSKDSGPMEPLPEEDESDLITTPLNQHPSQKANEPPDHEKLSTPLPSLKAGDETVSGRDEPLIDEIACALREWYTLLFVHLHRRRYQLFHLMKLQIEALHMGRRQLLAQTLSTEEAAKLRRDLVNRLVYGNYIQHLDIIVRHPDYGALVDSEVEIEAVDSRSWMSIIRMYVYQVKLAYLSPALNSPTGAQAILHLAEPSGSNALSSISANNQSKLCEPNQIPLNAHLPSLRNTRSNGPTSVDMLTSPHGTRAQNCSGSGYRPKFYHMMLEIKSFLASPCVEGEMMELYFSLYNKSESRFLTEEFCIILNHMGLPQNSNPVQSRHLQHMSTSYTSNPGGESHLGVGQDQKANPNELISLRTMFKDISPHDVQDSIFLVCRLVKNGAMKTAAGHSNHTATIQTMQETSHTLSVSPSGYEPDFNSLRNSFDVTSSDAPTLRQIGQPREAHGMTFTSLKSGSQSVRRPYGCAVVELAQFSSQSSQMLFGNTPNSAGMSRLTSQTTATPSNVFEPPTSRYTMNIFSPVSEASFPTLHEDIIASRTKEFFGSGNLTSNGPGHNTSSNKNHQPPQGLGPKNEAIEIQMKTYYGDTNTILKENSSMLSDVPVTSRLGFPDVVYPDDSRNEIYIKLWSGDFSNLSVGASKMISASGSGKNIEVAAELRTNTGQLLERMISRGSGEPKVTRYNSMVYRNNQTPTWGELMKLDISSETIEICHLFFTFRNRQEKSTRNNNGQTQMDKPFAFAYLPLFSSNRTFIPDGEHSLILFKYDEQSAMPEVYCRVRPTVIPGQAMPEITPALSKLLIPLKDSFIVRSFLCSTLHTQDEVLLRLMKWNTLLDDPDGLRQTLTKLKFASEVEICKFLRNIFDALFGVLVSRANAETKEYEELVFNALVTLLGIVSDRRFTNFKPVVDLYIDHHFSSTTASTHLLQSFQNLLINPTSPENAHPLRCSIKVWGYLMRFIVRSRELQRIKELNAPSSGLMCDAVESKFKVDISTLLGRINNLMTTDSSSIIGTQTLAVQHFASLMTELAKVFTPLELLEKANAFCESIKVTKGKIVMWKLLLQEHIVMSPIFDQADGRLQLVPKLTTWVKPHLGEFEDYLMFKPQDNDVIKHSARVTWLEGIRIAVAVIAVALDKLHEVLIDPAVCNDPGSLAQEHDNIEYLLGLLPKLLESFREFESPANVEIVQRLGTTASVISSVPIVFPATYPFSLLNSLPPKPNLNPSNSMSNPNDPNNNPSVGQQNNPPTVQNALGEIAVVMITMIMLAPSQMLKNHLELMLEVEGKINFGKFLSKIFKAFYSILKNASFPSNWLNINVISHKAILKLLEVVSKILQREFIPTTANEHDQSKEQMNLKRAGHVREEPSGSEEEERFDSNLWSDFFVLNHGLLSSKLLIIEEYPPQKRRVIWKLAGDIRDEGSKIFRAAWESIGDFSITIGSHNPADNSPNQDSQDNPDLNKEDTDQLTGPQHQINEVTRCGGYQVQFVPGFIEPLLELCLSHHDELRSNAVIVLYSVIVSEFNLNRDFTVIADEIIDKLDNLLGSSPNENLSNQMDEMSRASFVGQLRALFDHSPIIDRQEDPGQQGQQAGIEQALKTQIDLFLDSLTQFLDLLLSIRNLPPGDEFIDDRVIGTLKLMSFIRNIGRSGIYIHYVHKLVNFHAAHGNFVEAGLALRLHADLHEWDLNSIVDAMPGLSLPKQTVFARKEALYFRILDFLSKGKAWESGIQICKELQEQYEHVSFDYERLSEVLAHQSSLFLKIVKTDRYFSEYFRVAFYGQGFPPSVQNRQFVYRGYEWEKYAAFCDRMHNKHPNAQIIQSDAISTDELAYAEGQYLQITRVVAEPDRTTVVFKNPEVSSSVVSYYEHNATNTFSHSKPFNKDNVDPSDTVRMWVEKTFLVCEDVFPTVLKRSEILEIRVLEISPIENAIMITEQKTRELESLHRRYLALTKTSDAKLNTNPLSMALNSIVDTPAKTGIPAFRNVFLTPNYLEEHPQQVHPVQMLRQAIDHQTTVINASLQLHQYLCPPEMKAFHATLHKFFVKNFAEEIGRLPLSSSLNAELFGAQPEGSEDTQADLTQQPGRHDAYSLSRGNSFATGYDGNPMVNNGVYQPNGPARPSLSLSLGGLSPPITNPYGQHDSHAQPLIIGASPSIQTQFQQGYNFPSSSSSLNPSLSFNTMSGGQSSVGLSRSMTMTSSHQNPTGNYHEGQVMDKQSVMTVGGGGGEGYEFPGQQMEGANLVRTMSGPSSGFAESVYQSFNTSVHGNNRVGDLSTSGSSTLVSGSGPTNSGPGINQNPSGFGKLIRKGTGTILSHNSGSSRGSFQQNSSSISNQNLAVNQNPPSDRRSILGIRFGSRHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGK5</accession>
    <name>E3KGK5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08616</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83430.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327849.2">
        <property type="nucleotide sequence ID" value="XM_003327801.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KGK5"/>
    <dbReference type="GeneID" id="10532107"/>
    <dbReference type="KEGG" id="pgr:PGTG_08616"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08616"/>
    <dbReference type="HOGENOM" id="CLU_022063_0_0_1"/>
    <dbReference type="InParanoid" id="E3KGK5"/>
    <dbReference type="OrthoDB" id="2501108at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="310"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="378"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="438"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="624"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="262"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="330"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="625"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="646"/>
            <end position="660"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83430.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="740" mass="81516" checksum="CFECDFAB0EF7CCFC" modified="2012-04-18" version="2">MSTIFRLLGFNARQQRSQASSNGDSPSFDSGRWDSYQTALISENPGAEPLATVWLEASACKRRLALSSTASTLESNSALHHNKFISSSCTSTRYYSFEQPKPSSIFSEMSCSQRGPGQARNFSNASLPIVQRTGRVKLPQVVKPTRRKKSDPQADDSTRPPKPPTPSKLIRIPLKYPRAQLSTPTPPQSNHQLSRYRTQSLSCLKSTRDTNSNEGIDENPEMLSAAFFLPKLSMEPSIVSCTTTPGWKKPIDDNINTRLPDASRSLTTASSSSTRQPHSEGLHSPQAYTPPCPSGFQTWRPSLSEYHHSAVTHEDETIQDPCNPGNSNESQSSYGYRFSSSGGPDIGSATYSSTVPRSSHSSSGSQFSFISVYRSPESFNSQTRHNPCLPSEPPRHLRETQSNQINPQIVGIHSQTCSSTQQLELLCSTKKKYPSLSLSSAQPNPFPSFGRKPRSSSKSITYAPGDYSGFIDIEKPLKTSADMHTPSPYQREMLDIMAIHGESIFKPPVSTELMFKRHPSPCEEPSVHYSMNSSAEPSPKYRTLLKSSPRSDTFTQKRIERVAPLDSENLVGSGDDIQSGKTHLINQAEKSEPTTPLLPLFKSIAPSPRECRLKKIVLPIRPSTAGASTRSNGFPRRLSQSSRNKPPVEQHDPQETSSKKDKFEALLKASDASNGGTMKLSLSSKIDSIDESLHVPSTFLPQLRFSCESSSLTARRSSDCETRVTVNSSLKSKRSQQISF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGQ1</accession>
    <name>E3KGQ1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Spindle pole body component</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_08662</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
            <location evidence="5">Cytoskeleton</location>
            <location evidence="5">Microtubule organizing center</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the TUBGCP family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327895.1">
        <property type="nucleotide sequence ID" value="XM_003327847.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KGQ1"/>
    <dbReference type="STRING" id="418459.E3KGQ1"/>
    <dbReference type="GeneID" id="10532153"/>
    <dbReference type="KEGG" id="pgr:PGTG_08662"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08662"/>
    <dbReference type="eggNOG" id="KOG4344">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_289868_0_0_1"/>
    <dbReference type="InParanoid" id="E3KGQ1"/>
    <dbReference type="OMA" id="QHWEGAY"/>
    <dbReference type="OrthoDB" id="66546at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF33">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3 5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="9">
        <location>
            <begin position="115"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="8">
        <location>
            <begin position="511"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="824"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="776"/>
            <end position="803"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="19"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="37"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="828"/>
            <end position="841"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP83476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="955" mass="107785" checksum="64A2BE07E2976543" modified="2011-01-11" version="1">MEAEPLVGEHWQTDIYAGSSSSLSDSTPSSDWNSDSEEGKPDPASDPEERLSKSITTKEDLPKVPVDQLSEPVDLRDQESLNAGVRKLEEMRRNAYWLKSETEIANTPLPELIAIREVLAALLNTGGGLIYNVPSNTKDPMTLSAHVPTLLDKSRTSSLSLLSSFLPVLSSLRKLRQLTQPNEIHLSPGRKTLTEEAFADAVLGLLTHFDVFLTSIELELLNLPGRHTDSDESNVQFESVFSLMRLHYQLDQNGWIVLFTALADAVPDPLASAPDNQHSLTKLLMDNLYRIACQFDSHCKSANAVERVKVIFLRTCEPVWRWVGDWIIHGRLPASPGFVGEPKRQSNEEFLIQPTGSGGSNTAENWWEDRYVLKPSAIPEFLNDFVAHILEAGKATALSRILDILSDNHEIPDWPKLEELVGVSAPLDSHPSQPTDPQPVNILRLHLRHLAFSSRSSKTVPRVHSAADVVESEVSFHQDLYSQINQHLSPLLAQTHSRLHTRFSSPLKLSSYLKELNEFFLLGSQASVQFLKKTIEDASESSRSSLLTTAHLHLNSTNWWDDQVMNGNLSKALEYAPSSLLSASLPKLQISPSIRTEADPLKCLESIHFSLAIPTPLNYVLDNAHVMKTYNDCFVFLAQLARASWTLDSLIWIKPSIPFGSFAASKQFEQTPEAKEFCRLKNRLAWFVNLISDYAFNSVIFTWKIRFIQALDLQVGDLSQTIRNTRHWLARLAGLMFLSEQCQSLNKTILQVLQLCTRVHEVWTRFNRQVNTLKLIEDISETYVNTERRRRRIERRKRRMRNEQYSLLEEESRHIDPLDVDSMVPLDAGDEEHEEDPELDEQTGLAGNLSSTTSLDQTAQNLLHLVGSSVPDDDALEQLQSMNVEFEKLLLSFQRAIGKLCRTTLLKSNNYFPSTSSTHPDLRHQPLEDSGFGLEGSQDLDTLTLLECRLDEWIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGS4</accession>
    <name>E3KGS4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphate system positive regulatory protein pho81</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_08685</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83499.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327918.2">
        <property type="nucleotide sequence ID" value="XM_003327870.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KGS4"/>
    <dbReference type="STRING" id="418459.E3KGS4"/>
    <dbReference type="GeneID" id="10532177"/>
    <dbReference type="KEGG" id="pgr:PGTG_08685"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08685"/>
    <dbReference type="eggNOG" id="KOG0504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1161">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2421">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002842_0_1_1"/>
    <dbReference type="InParanoid" id="E3KGS4"/>
    <dbReference type="OrthoDB" id="1577640at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14483">
        <property type="entry name" value="SPX_PHO81_NUC-2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051631">
        <property type="entry name" value="Ankyrin-KH/SAM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057506">
        <property type="entry name" value="C2_GPCPD1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004331">
        <property type="entry name" value="SPX_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23206">
        <property type="entry name" value="MASK PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23206:SF7">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25329">
        <property type="entry name" value="C2_GDE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03105">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51382">
        <property type="entry name" value="SPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="SPX" evidence="5">
        <location>
            <begin position="38"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="416"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="449"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="482"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="547"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="domain" description="GP-PDE" evidence="6">
        <location>
            <begin position="877"/>
            <end position="1187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="649"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP83499.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1189" mass="131393" checksum="635F41FD82860A33" modified="2012-04-18" version="2">MNSTDEVASGWRNLTAVTEVIVSNYTFPYLFVATWLVMKFGKQLQAQQIPTWTAYYLDYKGLKKIINSLAKGRPADAALLAAGISPAIVTSTATNSHQQLAADQQLQLLPESYNDPRASPENNLKLHKAAFFFKLERELEKINEFYLQKESDLKVRLRTLIDKRKVVQCSRTRRLTKDNSSFATLYEGFRHFEEHLRKLQAFVDINQTGFRKILKKWDKRSKSSTKELYLSRQVEVQPVFNRECIAELNDAVAANILELEELLVDSEDRQPRSHASDPSADQISTDWKQVLDHPGSQRTAPQTAVEIDIIEFEELETNLIQAVKADNREQVAELLHLLPLPSSPLEQTTSVRSRLGRILWKAALSVEEPQAQVVSKSHSAQGSSNRITILTPSTAMVENDLIPSSRLDFSFVDDISGRSCLHEAASAGRTRLIRQAIEHKVSVSAPDVYGRQPLHYAAMNGFAQACQLLLTYNADPYCLDHDGYTPLIYSVTKGRVDCVRILLDSGISLEPVRHAGLLPLSLACQHGHGDIALLMLKRGSQVLANQEGMWPQHLAAREGHAALLRLLADAGANMDERDTFSQWTPLFHAASEGHAECVEALIASDCDCQVLDESRKCPIHYAAWQGHIGCVNLLLAKQRSTRLTGMVKQDSEMTAGPSTGTQRAPSAPNGALPSEGIKSGSLMTGSSTGIEPADADVDLIPDLSLPPPIIPFRIYGHNYLDKKALVQLYLGHPNTARSTRKAIQLYGQPELTSLKLVISNRSDVSSVVHSTILPLDYERESFSFHTDSVDHFALEFELFPTFGTKIMGKAVGLPSNFEGPCNQKPCVLPLLDKYLKVIGEVAFELNVIRPFRDVQLSFGGRVETYWKAMTNFSASTIQGDSPMGSPFGTPSINLASNAFVTASSLSGKYVQVAVQVTRDGVPVVYSGTTLNVHGFEIPMYNVTIAQFMKLANDLSRTFDSCSLPSDDESPHHWHEFLKNSMVTLESVLERLPISLGLDLEIGDPGISQHGSLDLNTFVDAILKTIYDSTAKTTPQNQSHRRRRRRLVLSSFEPLVCTALNWKQPNYAVFFASDCGVSGWNESTGQLLTPSGSAIETSTCHGNYELDRRRRSILEAAKVAKANNLLGVRLNATLLLRVPSLIQSTKEMGLLLTSFGPESKESIGLGVDGYMSSSGVFEYASHVEPDLIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>E3KI53</accession>
    <name>E3KI53_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_09691</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the RRM U1 A/B'' family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EFP83978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328397.1">
        <property type="nucleotide sequence ID" value="XM_003328349.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KI53"/>
    <dbReference type="FunCoup" id="E3KI53">
        <property type="interactions" value="658"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KI53"/>
    <dbReference type="GeneID" id="10532670"/>
    <dbReference type="KEGG" id="pgr:PGTG_09691"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09691"/>
    <dbReference type="eggNOG" id="KOG4206">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041869_0_0_1"/>
    <dbReference type="InParanoid" id="E3KI53"/>
    <dbReference type="OMA" id="VRMIPTK"/>
    <dbReference type="OrthoDB" id="266020at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030619">
        <property type="term" value="F:U1 snRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12246">
        <property type="entry name" value="RRM1_U1A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12247">
        <property type="entry name" value="RRM2_U1A_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000039">
        <property type="entry name" value="U1 small nuclear ribonucleoprotein A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000029">
        <property type="entry name" value="U2 small nuclear ribonucleoprotein B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10501">
        <property type="entry name" value="U1 SMALL NUCLEAR RIBONUCLEOPROTEIN A/U2 SMALL NUCLEAR RIBONUCLEOPROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="79"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="12">
        <location>
            <begin position="241"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="183"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="62"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="188"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="205"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP83978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="315" mass="34733" checksum="AE0CB58319081CA4" modified="2011-01-11" version="1">MEVINAQVPQPEAIQQDQEAKPEMEKTITTASASASATDLKPTENSQIPAGLTDTTIPTNSSDEKESEQVDKIPDDATETVYLNNLNERVKLPALKQTLKNLLKNFGPVLDVVAHRSVRMRGQAFVAFPDREMAAKAVKEVKGFPLYGKPIEIAFARSPADCVVKRKTPDDFEAHKAERLLKKKRSRRENPLRKKAVEAKEAARKAASTAASAGPMASSVPPASSNRKIVQMPDEYQPPNKILFIQNLPENAGKDALEVLFKQYPNLVEVRTIPGRSNIAFVEYVDATSSGVAKDALHNYKFDGEHKIKVTFAKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIL1</accession>
    <name>E3KIL1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_10514</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84136.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328555.2">
        <property type="nucleotide sequence ID" value="XM_003328507.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIL1"/>
    <dbReference type="FunCoup" id="E3KIL1">
        <property type="interactions" value="233"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KIL1"/>
    <dbReference type="GeneID" id="10545314"/>
    <dbReference type="KEGG" id="pgr:PGTG_10514"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10514"/>
    <dbReference type="eggNOG" id="KOG0257">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017584_4_0_1"/>
    <dbReference type="InParanoid" id="E3KIL1"/>
    <dbReference type="OrthoDB" id="2414662at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016212">
        <property type="term" value="F:kynurenine-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000024">
        <property type="entry name" value="Kynurenine--oxoglutarate transaminase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051326">
        <property type="entry name" value="Kynurenine-oxoglutarate_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43807">
        <property type="entry name" value="FI04487P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43807:SF20">
        <property type="entry name" value="FI04487P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="7">
        <location>
            <begin position="85"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="8"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP84136.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="477" mass="53634" checksum="C6CD629D6D0CCE66" modified="2012-04-18" version="2">MIRRSLLGSATTNTRSISNSQTQQHLLNQPAQHPRLRLLQLSRRRTSTGKRMSSITVSEKVQPELDKGLDCWSVFSPASGHMPPDSLNLGQGFMNWAPPEFMKEAAKNSIDEIAANHYSIPKGRVNLRKAISKHYSESFNLNRALDFESEIVISSGANFGIYASLLAFCSKDDEVILIEPFFDQYIRNVTFCGGKPVYVPLRPPKNAGTQNVSSKEWTLDIDELKAALTPKSKVIIINTPHNPVGKVFTKDELNAIGKIAEEHNLFIIADEVYDCLTFNEPHVRIASLSERLWKRTVTVGSAGKSFAATGWRIGWCIGPAEILRPITAAMTRITFCSVSPLQEALATGFEIAGKENFFETQRAQYLERRDLLLRFLDQLGLPYTVPDGSYFVLVETTKIKIPEEFEILELIKSRSRDWHMCWFIAKVAGVVCIPPSDFYSSDHVSIGQNFIRIAFCKDLDTLKEAGQRLQKLKPYIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJW2</accession>
    <name>E3KJW2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10746</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329006.1">
        <property type="nucleotide sequence ID" value="XM_003328958.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJW2"/>
    <dbReference type="GeneID" id="10546176"/>
    <dbReference type="KEGG" id="pgr:PGTG_10746"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10746"/>
    <dbReference type="HOGENOM" id="CLU_1278173_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJW2"/>
    <dbReference type="OrthoDB" id="10272405at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173653" evidence="1">
        <location>
            <begin position="23"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="208" mass="22258" checksum="F4A6064EE0100671" modified="2011-01-11" version="1">MNTTFFTSLLAVILIGLDSSAARPAFGRVGDLASHDVESLPRFLENGGLQGPMHEDSVFDSGYLSRYNLQSPTGGMKTVQKTSPNLLGEPNEAGGSALLENARLVLYRILYGTGEKTETDGAITADQIMRNMMSFEAIPTAGKPHSGNLVIRPSESKMTTTATDGDSPKSTLSTHQIIRNMMPFEAIPLEEEPHSGLSEGKVITSATN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KK33</accession>
    <name>E3KK33_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_10817</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329077.1">
        <property type="nucleotide sequence ID" value="XM_003329029.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KK33"/>
    <dbReference type="GeneID" id="10546327"/>
    <dbReference type="KEGG" id="pgr:PGTG_10817"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10817"/>
    <dbReference type="HOGENOM" id="CLU_1294974_0_0_1"/>
    <dbReference type="InParanoid" id="E3KK33"/>
    <dbReference type="OrthoDB" id="2505208at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="98"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP84658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="213" mass="22856" checksum="547B175A181115F9" modified="2011-01-11" version="1">MTTNQQQLPRTSSFNHLFGGAILDGLKLSSIVIPGGLLIHQLRQHMKLSQSLSTSAHTTASAPLPPSSSSTFSARSSPTSKINSLNYRLTHFLKWNGLLTFGFSLLYAAVLTTSSSLIQHNIKPLSTTTTRNDKPKSQRSSHSNDYSVIGGTLGGLITTTIFWNRRLFSLLSIIPGGIGLGVGAGLSTAYLHSLQSRSPNQPSSIADPEQQKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>E3KKY3</accession>
    <name>E3KKY3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">[RNA-polymerase]-subunit kinase</fullName>
            <ecNumber evidence="3">2.7.11.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_10429</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>[DNA-directed RNA polymerase] + ATP = phospho-[DNA-directed RNA polymerase] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10216"/>
            <dbReference type="Rhea" id="RHEA-COMP:11321"/>
            <dbReference type="Rhea" id="RHEA-COMP:11322"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43176"/>
            <dbReference type="ChEBI" id="CHEBI:68546"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.23"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.23" evidence="3"/>
    <dbReference type="EMBL" id="DS178292">
        <property type="protein sequence ID" value="EFP84958.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329377.2">
        <property type="nucleotide sequence ID" value="XM_003329329.2"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KKY3"/>
    <dbReference type="GeneID" id="10526933"/>
    <dbReference type="KEGG" id="pgr:PGTG_10429"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10429"/>
    <dbReference type="eggNOG" id="KOG0600">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005076_0_0_1"/>
    <dbReference type="InParanoid" id="E3KKY3"/>
    <dbReference type="OrthoDB" id="28397at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004693">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008353">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000415">
        <property type="entry name" value="CMGC/CDK/CRK7 protein kinase, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050108">
        <property type="entry name" value="CDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056">
        <property type="entry name" value="CELL DIVISION PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24056:SF233">
        <property type="entry name" value="CYCLIN-DEPENDENT KINASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="429"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="342"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="377"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="777"/>
            <end position="1315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="21"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="171"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="199"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="223"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="234"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="263"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="312"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="395"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="777"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="801"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="890"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1075"/>
            <end position="1094"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1117"/>
            <end position="1129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1151"/>
            <end position="1165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="1177"/>
            <end position="1193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1197"/>
            <end position="1207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1241"/>
            <end position="1250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1251"/>
            <end position="1262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP84958.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1315" mass="145169" checksum="389A49EF076E141B" modified="2012-04-18" version="2">MSSVQLEEGEVEDNRLNTRLSSSPTNNKNNNNSNNNNNNNTNNLTISPSSSTNSHHFNSLPPKPPTDLSKIKTAINPNAINALSRPIHRSPNNRNRSPVGRSNYRPTDYPSNNNTHNNNNRLNNHLSPSSSRSHHSPAHQSRPPESDSIKPTYLSPDQNRRQTRNPRSNRSHSPSLSPTHSASRSRQRNRSYSRESSINHHHHHPFKNRRSKSPRPSPSSHSISRHTRNHHRSPSISRSPSRSQQPPNKRQRPLSPSPLPSSHPTQTTDRPINPSQKELPSSPSKPQNEGSFDIQPSNRNHHDHILHPPPSTQAQSNPQLSSLPTTQLVPSSQELLENNLTRSYSNDPENSQDPSSNPSNHPRSIFQNYQPTFFFCQSPKSRVNPEPSLTKENGDSSQEITRTESSQVEPVSNGTTKKCFVGVSSIRSYELVEKLGEGTFGEVFKGIYRGRLYAQQQKLQASAQNTLRDGSESCDDDFETDDRLIETKSKVKSGMVVALKRIIVHNELDGLPITALREIRILKSLDHPNIVPVIDLAFSRGDKNLLKRGNTYMVFPYIDHDLAGLMENKSITFNVSQIKLYSKQLLLGTAYLHRNKILHRDLKAANLLISNEGQLMIADFGLARSIEQQHNNKKREYTNCVVTRWYRPPEILLGNRRYGTPVDLWGVGCVIAEMFKGGPILTGSTDVNQCELIFRLCGSPTSESMPGWENLPGCEGVRSWTSKPRRVREEYERISPELADLLDHLLVLDHSRRLTAEEALDHDWFWTDPMAIDPAKLPHYEPSHEYDRRKKQEQAKKTHQHQLASFTEIQGTSSMVPNKINGSQPAAKPHRLPLPASNSRPPPAFLNENPFPSDPFKHPIGPPNPGNRIVADPGPPPPFHRAGLNHAVNPYSSQGSSVMMNQNEGRGDGDHRQQPHSSSRRMRPEPNRGGPMGSHPYPGPPPNLPGGQAPPLGNSAPSIASYGHASLPSRPVTSFDRGPHDPMRLPKPGPPMSMIGGMNGAIGGMPPGPPYAHSHGLSGHPEPYALGPPPPMLPEHGSPDERGQRGEPFPKMGYSAPLSSGQPIRDTYGGGGGFRGRDGGRADGRFRSSHDRWTPEPNSQLGGPPPPGISPYSNGRSGDRRQAGRGGERRFRHGGGGPPAANGPHDGRENFQYNNNPATPRNSSSQYYGPQNGPSGGSPPNPYSSYTYPPPGEPMATPTSNPFSHPANNSNSNNNGRGAHQNGHPNQRWENKPNTSLDFRSPPSHNNNPYNHHHPHHPHHQPHLPLSPPNLPLHHHHPHPHSPPNGNGAAGGPSFYNGNGTAGGPSFSDEPRLEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLK7</accession>
    <name>E3KLK7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">CSC1/OSCA1-like N-terminal transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_11351</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP85182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329601.1">
        <property type="nucleotide sequence ID" value="XM_003329553.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLK7"/>
    <dbReference type="GeneID" id="10527165"/>
    <dbReference type="KEGG" id="pgr:PGTG_11351"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11351"/>
    <dbReference type="eggNOG" id="KOG1134">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_814176_0_0_1"/>
    <dbReference type="InParanoid" id="E3KLK7"/>
    <dbReference type="OrthoDB" id="1689567at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005227">
        <property type="term" value="F:calcium-activated cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045122">
        <property type="entry name" value="Csc1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027815">
        <property type="entry name" value="CSC1/OSCA1-like_cyt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032880">
        <property type="entry name" value="CSC1/OSCA1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018">
        <property type="entry name" value="PROBABLE MEMBRANE PROTEIN DUF221-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13018:SF5">
        <property type="entry name" value="RE44586P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14703">
        <property type="entry name" value="PHM7_cyt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13967">
        <property type="entry name" value="RSN1_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="199"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like N-terminal transmembrane" evidence="2">
        <location>
            <begin position="48"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="domain" description="CSC1/OSCA1-like cytosolic" evidence="3">
        <location>
            <begin position="245"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP85182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="341" mass="39045" checksum="D413E41E5777E4A2" modified="2011-01-11" version="1">MGSRMNSTSSSNPLMEFHPTHLFNQSDESAPPGSPVDRNNRLKHGNWFETQLVLSLAIGSLAFLTFCYSRARSTLTFAPRTHLSSFSPHPVHLKSGFFSWILPTLKTSEHTVLQIVGLDAVVLLYFFKLGFFYFLTCTIFAFLFLVPINIHENGTTEGVPPDPPSPKLLRSLGEQVAQPLNFLFSDDKYNWPDLTKNVTLYHCVHLGFTYFFSLLLLRFLKITYLRFIETKPIFSLAHHSSVPLRTLIIENLPLHLQNDEALFEYFNDLLRFKVESVNVVRDVSGLVPLLSRRTKALINLEEAWAKWLGNPVRGEAALNYHPEEELARIVYHPRDDQTPNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KM32</accession>
    <name>E3KM32_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178294">
        <property type="protein sequence ID" value="EFP85381.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329800.2">
        <property type="nucleotide sequence ID" value="XM_003329752.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KM32"/>
    <dbReference type="GeneID" id="10543929"/>
    <dbReference type="KEGG" id="pgr:PGTG_11550"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11550"/>
    <dbReference type="HOGENOM" id="CLU_1836120_0_0_1"/>
    <dbReference type="InParanoid" id="E3KM32"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85381.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="140" mass="15958" checksum="E7C9979D96C6F3EA" modified="2012-04-18" version="2">MSFSSEQQPKPTPQSKLTPTLPPAETLTELLLNSEIQEISEEEFLTSLIKKRDHALATSKSQFPKSIKEDFARIVLRMDCILKDYESNKCLPTDEAMHKTKHHSGFCFCTPKTKKDAEIRTKIASTRSNYFSHVNHTKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KMS8</accession>
    <name>E3KMS8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11106</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EFP85777.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330196.2">
        <property type="nucleotide sequence ID" value="XM_003330148.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KMS8"/>
    <dbReference type="GeneID" id="10544352"/>
    <dbReference type="KEGG" id="pgr:PGTG_11106"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11106"/>
    <dbReference type="HOGENOM" id="CLU_033090_2_0_1"/>
    <dbReference type="InParanoid" id="E3KMS8"/>
    <dbReference type="OrthoDB" id="2500816at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="441"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="444"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="458"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85777.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="469" mass="54689" checksum="D1972579819E47CE" modified="2012-04-18" version="2">MLLHWIVRSMDGDEASESESSSRGQQTATHRVIRALKELLDYYQFSLSQSFCHHVENISIDRLDWKRKILNDLQTSLLPLIKRQISVLLNSLDLSHMPIHACPNPELTLQIVSEIEHTLDETRSAVELIALRQPLPASSHDHHLEKFKSIRSLRLMRKTKTLIQENLCDLFEACALLIWEHENNDPEDLGYQAHASKRRQRITMMAADYQNRVDRLIEWSKLSDFGFLQHEWRLTSYKLSETLEVLGKMTNESYSTSSNNDKEDDQIAEDRSVEDEETKRLADHRERANQIAQSFIPFIKLGRIFYDKLSKTAINRLPFELYTEMSSDQLELLANRTSWIESSINDLMGELSRLHNSSEDFERRISWMRNEAIMTLYRLDRTLLDLSLNLIPVHTTTSTAYSSSPPQSHFKTWFLPLKKQFRAACVHFLRTINIGFGDLLGQDNESESEEDENENENENDRRVEEDPIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3KMZ8</accession>
    <name>E3KMZ8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_10963</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EFP85634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330053.1">
        <property type="nucleotide sequence ID" value="XM_003330005.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KMZ8"/>
    <dbReference type="FunCoup" id="E3KMZ8">
        <property type="interactions" value="140"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KMZ8"/>
    <dbReference type="GeneID" id="10544199"/>
    <dbReference type="KEGG" id="pgr:PGTG_10963"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10963"/>
    <dbReference type="eggNOG" id="KOG2645">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017594_4_1_1"/>
    <dbReference type="InParanoid" id="E3KMZ8"/>
    <dbReference type="OMA" id="DEYVSRD"/>
    <dbReference type="OrthoDB" id="415411at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047429">
        <property type="term" value="F:nucleoside triphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009141">
        <property type="term" value="P:nucleoside triphosphate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16018">
        <property type="entry name" value="Enpp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.180:FF:000003">
        <property type="entry name" value="Type I phosphodiesterase/nucleotide pyrophosphatase family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002591">
        <property type="entry name" value="Phosphodiest/P_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151:SF120">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10151">
        <property type="entry name" value="ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01663">
        <property type="entry name" value="Phosphodiest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP85634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="602" mass="68482" checksum="3BD0595B62BA907B" modified="2011-01-11" version="1">MVRSRKGSTVQRSNPLGPTNLQTGIRTDDDDDEEGYIQTQQQHQAETNQTASLEEPLLKSDNHQASRQKKNDLEPFPTSTFLISSLIALGLFLLFFCLAPSISPLFPRATTPHLSATTTTTGTATDQSNPPTSSKKPTSKFVKPSAPPGSITDNTDNWANQGGGWKDSDPDAEDKIAEIGAPLFNGTHRFKKTVIIVSLDGFRAEYLERGLTPRLTTIREKLGISADYLEPQFPTLTFPNHWSMLTGLYPESHGIVANDFRDSKLGKSFSNIDPHRSWDPVWWGGEPIWATATKHGIRTAISMWPGPPYTIDKTRPTYWRAFEDRYKWWKKIRQLENWLDLPVKDRPELIALYVPDVDQMGHQYGPTAEKFNQSLQSVDRFVGALYDSLQARNLSEIIDVVMVGDHGMMATSDQRIVYLDQVLGHTYFNQIQFQDGWPAAGLRFLPEVDTRPILERLIEQSKILPGFEVYTHQTMPQRWHYTANDRIAPIYVFPKPGWVISNTHEHQVVMNGTYDVKGVHGYDNEEESMRSIFIGYGPFANTIKNNQLRSIRQSTTHPLVVGRLPKFSNLEIFSLISKLLDLDRFIDFNQRNCSIGFWNQYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3KN87</accession>
    <name>E3KN87_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EFP85723.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330142.2">
        <property type="nucleotide sequence ID" value="XM_003330094.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KN87"/>
    <dbReference type="GeneID" id="10544289"/>
    <dbReference type="KEGG" id="pgr:PGTG_11052"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11052"/>
    <dbReference type="HOGENOM" id="CLU_009635_2_2_1"/>
    <dbReference type="InParanoid" id="E3KN87"/>
    <dbReference type="OrthoDB" id="2505003at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF5">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="440"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="459"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="474"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85723.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="570" mass="64404" checksum="7EB7FE748806015D" modified="2012-04-18" version="2">MSRKSYGLGLSSQPNGPGNELRRSSRVSSTTSRHPSIILPPSDSRRSVNRTLPATQKSKKKRTRSVSTSVTEASDQEVSTNKNSSKNQKKKKQKKKTITQVNDSDSGSIIDLREDSEPDNSKIPAAKPCAEKYDSPLEFFGKPFYPDKTTEEERKKKPITYNCKWCKKRVRGGHNSDANLQKHRDGSNQAGRDGSGCPNRSLAIQAGAKLPPTVNETKAQAEKNGTGNKKLTSFFGRTEKFDKKILNQNLMIWQTRNALPWSRIEDIDLQAGFHYCQPDAILFKRKWVAAEAKQLYISLQGAMLQTLKKSSSKFTLVHDAWTTKGNRYAFIGSSVAYINDEWEFNMMHLSLKLTTDSASSNNTMATRVYDILADKNGTYSFDWRPERMHIRCFCHKIALIVNAGLAELGMIAPPPAKIKESVLGTFPYSDAMETITEVEEEMEEDNNGPASCSVGEDNINIDDDSEPEHDEEELSKLLEQQEKDAGNLDEEQREHQATNRNESNSLDRLTKKLDFVVRKITATSAWRQVFIRKTKKKGLKLRSLIPGYGIRWNIKFESRERAYEAREVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNG2</accession>
    <name>E3KNG2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11593</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178297">
        <property type="protein sequence ID" value="EFP85837.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330256.2">
        <property type="nucleotide sequence ID" value="XM_003330208.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNG2"/>
    <dbReference type="GeneID" id="10544417"/>
    <dbReference type="KEGG" id="pgr:PGTG_11593"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11593"/>
    <dbReference type="HOGENOM" id="CLU_518888_0_0_1"/>
    <dbReference type="InParanoid" id="E3KNG2"/>
    <dbReference type="OrthoDB" id="10682236at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="484"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="486"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85837.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="526" mass="58110" checksum="FC9231C32541DD0D" modified="2012-04-18" version="2">MNPEPPDVEPTGQAGQSTSLGGGEQGGQSGTPGGGDGEREHSWISTIIDKIIPNLPGAGNRNTSAGNTATMDSQPSGTLNTDGMTDRSRLDPDIVIEHARGARASVEEQGNRIHEERRARGIAKKATLNEHCDKYSRGIQHFIKFFLGGPTIPHDYPPSPTSEELEALYWVDRRSTIIIEQLERLRSSLSARSTAEQDFFLAQAEKEIRKNIPLPTFHPAPRKGVLSGGRPISTQTKADVERALALAGISRFTFEWNVPHDEDSIWNSAVIEVMGKKSVEWLGRSIKINEEEARHAAAIIKRWLETKSREIQQYGGMNKEDYTTLKSQKATKALLQSWRKKIKEQRYSMADKVFRDIPQLAVVLEDNDCHSDIEEGSGGNNPVSLFPPWRSVSLTKILHNLDRMVQAQATHHKTIKTNKKMYARSARNHAKTVGGAIGVSRDWPIDCYDQAFWKGLSKFEQDTLSKTPAIKISDIDEVLAGLCRKGSNTQPSGPSNTSASTWGQKRLQPHEAAGSDAGPSRPMNVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNS7</accession>
    <name>E3KNS7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_11708</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178297">
        <property type="protein sequence ID" value="EFP85952.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330371.2">
        <property type="nucleotide sequence ID" value="XM_003330323.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNS7"/>
    <dbReference type="GeneID" id="10539951"/>
    <dbReference type="KEGG" id="pgr:PGTG_11708"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11708"/>
    <dbReference type="HOGENOM" id="CLU_461607_0_0_1"/>
    <dbReference type="InParanoid" id="E3KNS7"/>
    <dbReference type="OrthoDB" id="10275929at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="442"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP85952.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="604" mass="68843" checksum="FB15E2F77A3A2924" modified="2012-04-18" version="2">MRRPIFEVYSTMFLIGTISSRLIPRLRSEEIFNAAFVCPDLNAPPPVVDEVLPLRPIVHPDSVAIGIPSEARQINSSQAPNLNANLPAHPAGLEGDHGECSAQAKVKSFFEQAGCSAPKKRLSGIPGPSEPRMERVAETPAGTQPEILAQHVTDQAGSLKTVVIYHYSLGEPTLKPLSPSGPAVDVQSSSSRCEPEGSTPSRGRNTFVAQDLYSRSSSKRKGDHLETNGEAQKKFKQVEQTLTEIQKGSPLQSTQNLMTVYPTSFNTKGSSPKSKLQVEEEKALDIGQQTSNLDEEEEAASQISTGLFKVCNWDYLQDVLAQDEETTLEEPTEDYRAEWVFEQLNRCKTPSSREEVFWIPRSQSESFLEIFRRLEGIFPANFDDDIRREAVGEFFLSLAEKRLALSKNIILKDQIIKPMREKMLAIPSVKLTPRRMNRIDKIVRYIEIISTMVTFLIISYLTLFNEHEPGFLRTETVDNLLNFIKDFWTKVREQDSDFLDGHPWAKVNIEIMRMTSRIRGEKDYTAFVYQKRCQMAHAWHILEYWVEINKKSKSPHLCSRNSRNIYQIITIIIMSQNYRRIFNMVSEKKKIKALMQRTSYKQKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPY0</accession>
    <name>E3KPY0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">(2E,6E)-farnesyl diphosphate synthase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Dimethylallyltranstransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Farnesyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Farnesyltranstransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Geranylgeranyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Geranyltranstransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_12321</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178299">
        <property type="protein sequence ID" value="EFP86365.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330784.2">
        <property type="nucleotide sequence ID" value="XM_003330736.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KPY0"/>
    <dbReference type="STRING" id="418459.E3KPY0"/>
    <dbReference type="GeneID" id="10540379"/>
    <dbReference type="KEGG" id="pgr:PGTG_12321"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12321"/>
    <dbReference type="HOGENOM" id="CLU_014015_6_0_1"/>
    <dbReference type="InParanoid" id="E3KPY0"/>
    <dbReference type="OrthoDB" id="6921389at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004311">
        <property type="term" value="F:geranylgeranyl diphosphate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF44">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01017">
        <property type="entry name" value="Polyprenyl_Transferase_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00032380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP86365.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="348" mass="39459" checksum="621BB2D9A9631CD6" modified="2012-04-18" version="2">MAVSNGINNVEVDSVQINGIEERYSNFFERMKQNNPDWSEPQERILLEPFTYLTSTPGKEIRSMMIDAFNHWLSVPTEQLNIVKRIVGLLHTASLLMDDVEDGSELRRGIPVAHKIYGIPQTINSANYVYFLAYQELSMLHPSQDSSASNLWKLINEELLRLHRGQGMDLYWRDSLTCPTEEEYIQMVKNKTGGLFRIAIKLMMAMSPLQQIPDYVPLVDLIGIIFQIRDDLLNLSSDYTVNKGFCEDLTEGKFSFPIVHAIRADPSNHQLLNVLRQRPTDDGIKSYAVSYMKEKTKSFAYTRLVLGILEAQADKEVARLGDNPALRSIFSMMHVAPSPPQSTAPSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQ87</accession>
    <name>E3KQ87_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein BIG1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12418</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178300">
        <property type="protein sequence ID" value="EFP86462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330881.1">
        <property type="nucleotide sequence ID" value="XM_003330833.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQ87"/>
    <dbReference type="GeneID" id="10540477"/>
    <dbReference type="KEGG" id="pgr:PGTG_12418"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12418"/>
    <dbReference type="eggNOG" id="ENOG502RDP7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1034704_0_0_1"/>
    <dbReference type="InParanoid" id="E3KQ87"/>
    <dbReference type="OrthoDB" id="2499821at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="231"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="277" mass="30317" checksum="FB91D8442C53B0FF" modified="2011-01-11" version="1">MHNSNFRCTYARIFSTVLLFGLPNKNHAFMETSSFLAWSNAPGASLQEFFGQGLPDRGFQAATDQLTFASRSEPLCPTGASSLTIFDLSESDKRSELNNGFLSSQTITQEFNALPEPSRFWTTHFPISLADRWVAELPSIWSGLCHGQVSVIKVKTGEDLDAAFLKASQEGDQPRLVILTTSPPPLDASSRLIERRGLSENISSSSKPISSEALPDPKTGVFWRYNFLSDYLIVGVLVLILLFIPPVVLGAFALQSIESPKGLKSKMVGQVSEVKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQC1</accession>
    <name>E3KQC1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12452</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP86496.1"/>
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    <dbReference type="KEGG" id="pgr:PGTG_12452"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12452"/>
    <dbReference type="HOGENOM" id="CLU_2293062_0_0_1"/>
    <dbReference type="InParanoid" id="E3KQC1"/>
    <dbReference type="OrthoDB" id="2506420at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF5">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="21"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="101" mass="11512" checksum="14C50259FFB421FD" modified="2011-01-11" version="1">MAMQVGILAQAEQTPSETELSYQLPSKNKNIEKRQGQTLDKYVASATFDNHVLNQILVMWLVLSALPWTRIEDKLLRISFNYARPGIKLYSRTYAALEAPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3KTD4</accession>
    <name>E3KTD4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_13930</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178307">
        <property type="protein sequence ID" value="EFP87559.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003331930.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KTD4"/>
    <dbReference type="STRING" id="418459.E3KTD4"/>
    <dbReference type="GeneID" id="10538619"/>
    <dbReference type="KEGG" id="pgr:PGTG_13930"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13930"/>
    <dbReference type="eggNOG" id="ENOG502S4IY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_576373_0_0_1"/>
    <dbReference type="InParanoid" id="E3KTD4"/>
    <dbReference type="OrthoDB" id="15595at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018852">
        <property type="entry name" value="DUF2456"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28297">
        <property type="entry name" value="FUNGAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28297:SF1">
        <property type="entry name" value="FUNGAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10445">
        <property type="entry name" value="DUF2456"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="78"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="125"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="264"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="368"/>
            <end position="474"/>
        </location>
    </feature>
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        <location>
            <begin position="43"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="342"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="415"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP87559.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="474" mass="51839" checksum="6AED7286D3B792C9" modified="2012-04-18" version="2">MDRPFNAEDSDEIQTLGPPTLSSTDPDMVTALSLPTSPVDLEAQQSPPVSLSDPSSYPGRPAGLHPLAIPPLSLGQKLYIILPQMIGAAILDGAANFGIGCAMYLNAKNVRIWILKNNTVAGDMAVTIFIQGVLTFCIASGMVHVDLRKNKISAFQYPWPDTSWGAAKRLPPNEAQTRLGRLWRQFHNGQGFSRGLHFFSGSDVNDIFDSRLSRKQFTTRLCWSIWKGSVLSLIFFFLIWPISIAIVTPIWVDENLGNGNFTAPLIKAVYGFVYGLLMNPVCAMIAMGSEDSVRAHREERQQARFQNPEKEAMKTTAAERVAAVDPSKAMSPPAQDAKPSMAQISNNPPQRVQSLRRSLDTLGLRGTGTVEFEDSQGPRGPGHTGLGWRSADVHRRRSLDTARRTSRGRYANPSGDLNRSTSVGSARTKSQPDTFGLFGPSGQLATPHEASHRHSSHSNTPGRMRTLSEHSAQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3KTT6</accession>
    <name>E3KTT6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein BTN</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_13437</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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        <subcellularLocation>
            <location evidence="8">Vacuole membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the battenin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="protein sequence ID" value="EFP87651.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003332022.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KTT6"/>
    <dbReference type="FunCoup" id="E3KTT6">
        <property type="interactions" value="95"/>
    </dbReference>
    <dbReference type="GeneID" id="10538883"/>
    <dbReference type="KEGG" id="pgr:PGTG_13437"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13437"/>
    <dbReference type="HOGENOM" id="CLU_785589_0_0_1"/>
    <dbReference type="InParanoid" id="E3KTT6"/>
    <dbReference type="OrthoDB" id="5965864at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051453">
        <property type="term" value="P:regulation of intracellular pH"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003492">
        <property type="entry name" value="Battenin_disease_Cln3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10981">
        <property type="entry name" value="BATTENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10981:SF0">
        <property type="entry name" value="BATTENIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02487">
        <property type="entry name" value="CLN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01315">
        <property type="entry name" value="BATTENIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="8" id="KW-0926">Vacuole</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="74"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP87651.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="353" mass="38303" checksum="6A2D90C72D756AB5" modified="2012-04-18" version="2">MAYKLSPIHQYSRELTSHPAHSTPRSEDSDSDSEQPPQAPLVIAPATTTTCTTLEGSSDIELAAILDKSSNFRFCLSLFLFGLLNNVLYVIIISAALDLVPSDVPTGVILVADITPSLLVKIGWPYFVKGQIRYARRILSCSALSFAGMMVIVIFSSVPLRLFGIFLASLSSGLGEMTFLQLTTRYQGQPGVSWFASGTGAAGVFGAALWWLLRHLGVQIGLGLSSVLPICLSFTYFFLLPPPSLLPPVTIEARGGYAVVPETDQTGLSTELPALSVKSPELTFAEKLRLAKPLLIPYMLPLFAVYFAEYTINTGIAPTLLYPPPQPNSHPVFSLVFKNLRDYYPSLAINLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>E3KUN2</accession>
    <name>E3KUN2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Histidine biosynthesis trifunctional protein</fullName>
        </recommendedName>
        <domain>
            <recommendedName>
                <fullName evidence="19">Phosphoribosyl-AMP cyclohydrolase</fullName>
                <ecNumber evidence="19">3.5.4.19</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="19">Phosphoribosyl-ATP pyrophosphohydrolase</fullName>
                <ecNumber evidence="19">3.6.1.31</ecNumber>
            </recommendedName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="19">Histidinol dehydrogenase</fullName>
                <shortName evidence="19">HDH</shortName>
                <ecNumber evidence="19">1.1.1.23</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="22" type="ORF">PGTG_13786</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>1-(5-phospho-beta-D-ribosyl)-5'-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:20049"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="EC" id="3.5.4.19"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 19">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + H2O = 1-(5-phospho-beta-D-ribosyl)-5'-AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22828"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="3.6.1.31"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18 19">
            <text>L-histidinol + 2 NAD(+) + H2O = L-histidine + 2 NADH + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:20641"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/9.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 3/9.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 9/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7 19">In the C-terminal section; belongs to the histidinol dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.19" evidence="19"/>
    <dbReference type="EC" id="3.6.1.31" evidence="19"/>
    <dbReference type="EC" id="1.1.1.23" evidence="19"/>
    <dbReference type="EMBL" id="DS178310">
        <property type="protein sequence ID" value="EFP87982.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332401.2">
        <property type="nucleotide sequence ID" value="XM_003332353.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KUN2"/>
    <dbReference type="STRING" id="418459.E3KUN2"/>
    <dbReference type="GeneID" id="10535041"/>
    <dbReference type="KEGG" id="pgr:PGTG_13786"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13786"/>
    <dbReference type="eggNOG" id="KOG2697">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4311">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006732_0_0_1"/>
    <dbReference type="InParanoid" id="E3KUN2"/>
    <dbReference type="OrthoDB" id="1703565at2759"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00007"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004399">
        <property type="term" value="F:histidinol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004635">
        <property type="term" value="F:phosphoribosyl-AMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004636">
        <property type="term" value="F:phosphoribosyl-ATP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06572">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11546">
        <property type="entry name" value="NTP-PPase_His4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1080:FF:000002">
        <property type="entry name" value="Histidine biosynthesis bifunctional protein HisIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1980:FF:000050">
        <property type="entry name" value="Histidine biosynthesis trifunctional protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1980:FF:000001">
        <property type="entry name" value="Histidinol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.1300:FF:000002">
        <property type="entry name" value="Histidinol dehydrogenase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1080">
        <property type="entry name" value="MazG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.810">
        <property type="entry name" value="Phosphoribosyl-AMP cyclohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01024">
        <property type="entry name" value="HisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008179">
        <property type="entry name" value="HisE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016298">
        <property type="entry name" value="Histidine_synth_trifunct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001692">
        <property type="entry name" value="Histidinol_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012131">
        <property type="entry name" value="Hstdl_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021130">
        <property type="entry name" value="PRib-ATP_PPHydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002496">
        <property type="entry name" value="PRib_AMP_CycHydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038019">
        <property type="entry name" value="PRib_AMP_CycHydrolase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00069">
        <property type="entry name" value="hisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03188">
        <property type="entry name" value="histidine_hisI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256:SF2">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS TRIFUNCTIONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256">
        <property type="entry name" value="HISTIDINOL DEHYDROGENASE HDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00815">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01502">
        <property type="entry name" value="PRA-CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01503">
        <property type="entry name" value="PRA-PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001257">
        <property type="entry name" value="His_trifunctional"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00083">
        <property type="entry name" value="HOLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101386">
        <property type="entry name" value="all-alpha NTP pyrophosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141734">
        <property type="entry name" value="HisI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00611">
        <property type="entry name" value="HISOL_DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 19" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="13 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="16 19" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="11 19" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="15 19" id="KW-0520">NAD</keyword>
    <keyword evidence="10 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Phosphoribosyl-AMP cyclohydrolase" evidence="21">
        <location>
            <begin position="249"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="107"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="422"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="422"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00005204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00008260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR001257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF01502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="EFP87982.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="889" mass="97713" checksum="1E4DE2D2BE86DED8" modified="2012-04-18" version="2">MVLFAHIPFLPYLDDPTRIPSREILIQLQTIVPILISAVHAAEILALLPAQVETYIYDDLDPVDLPEADRLETLIMLLDRGSSKVISQASFVKPLLERGVPHNRIIPRFSSSSSPQNLDHLSDLSSSSSGNPQSVMLEIPHNSDSDLSTSTTTLEKFLDFIDHQSKDHPTFLLDPHRQLESLLSSSSSSKRTTEELNVVQLIGSTDLIKMDSEQLIEQFLKSLKTDRPDGLYTTSVVSDSPACSLLGQVYSSLESIKKSIQTRKATYYSRSRSQLWIKGETSGATQECLRIRTDCDRDSLEFHVIQKPLTGFCHTQEISCFGPLGGLKQLESTLLHRLQTSTPGSYTNRLFNDEKLLRAKIMEEAEELCDAESPSDLTGEMADLLYFALCRCISKGVSLAQVEQKLNQRALKVTRRKGDAKPKWEAKINDGAKEDQRANPAPKPTPAEGPLRCQMIDLKEVPEQERASLLKRPMTDSRTMTGLVTPIIEMVKTRGDQGLLEAVQKFDRCGFESADQLTIRAPFAESSMQISPEIKQAIDVAYNNIYKFHEKQLEKERTPMVVETMKGVVCSRFVRPIDRVGLYVPGGTAILPSTALMLAIPAQVAGCEAISIATPARADGTISAEIMYIAQKCGVQEIVKAGGAHAIAALAYGTQRVSKVDKIFGPGNQFVTVAKMLVASDVHAATAIDMPAGPSEVLLIADRSCEPVFVAADLLSQAEHGIDSQVILLAIDLKAEQLQEIQNQINLQAHRLSRIEIIKQSIPKSFIVQVPTLDAAFQLSNHYAPEHLILHIKDPNQASHLIRNAGSVFIGPFSPESCGDYASGTNHSLPTVGFAKQYSGVSTMSFMKHITTQELTKDGLKELGPTVIRLANLEGLDGHANAVQVRLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3KWW7</accession>
    <name>E3KWW7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_14199</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178317">
        <property type="protein sequence ID" value="EFP88860.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333279.2">
        <property type="nucleotide sequence ID" value="XM_003333231.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KWW7"/>
    <dbReference type="GeneID" id="10541976"/>
    <dbReference type="KEGG" id="pgr:PGTG_14199"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14199"/>
    <dbReference type="HOGENOM" id="CLU_061626_1_0_1"/>
    <dbReference type="InParanoid" id="E3KWW7"/>
    <dbReference type="OrthoDB" id="2507774at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24383:SF20">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24383">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="11" id="KW-0732">Signal</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173802" evidence="11">
        <location>
            <begin position="24"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="101"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="170"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="367"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="374"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="386"/>
            <end position="398"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP88860.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="398" mass="44776" checksum="6F9E8F880370F8AD" modified="2012-04-18" version="2">MDPSCGAYGRIWLFLADLNLVLGCLPAPTHYGPAAFNSFMSPQNPYQSSLDFHSQLSRKQPTHRLRAWKNTHSRFGRKRLEKACTTSKSCPVPIPSIQQSAHVYSRHKHQRPHEPSQQTSQSWSTNQHQTSQSPCKQTVKHAVYTYEQDPRWLEDLKGEPYTTECLDHEEPSAVDYPNDSAEDHSSLQLRPPGDSMIGDQETACGSDKLKDEVDQDEIEFVDRSDLDINADPDYLEQQPKPAFDLKNYSAEDVDNWASTLSAYEFEHLRVMGPNARTEFFRHDYSHYQPNDTDKNNSYQQQLNYQVDNSKSNFYDDLDAHNPQLEENLDGSVGFVAENGLNDGSGFDNGGFDNGGFDNGGFDNGGFDNGGFEDSGFDDGVFDDSSFDGGFDDGYGSTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZ84</accession>
    <name>E3KZ84_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GH18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15572</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178324">
        <property type="protein sequence ID" value="EFP89609.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334028.2">
        <property type="nucleotide sequence ID" value="XM_003333980.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZ84"/>
    <dbReference type="STRING" id="418459.E3KZ84"/>
    <dbReference type="GeneID" id="10547662"/>
    <dbReference type="KEGG" id="pgr:PGTG_15572"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15572"/>
    <dbReference type="eggNOG" id="KOG2806">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_713973_0_0_1"/>
    <dbReference type="InParanoid" id="E3KZ84"/>
    <dbReference type="OrthoDB" id="73875at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF317">
        <property type="entry name" value="CHITINASE 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH18" evidence="1">
        <location>
            <begin position="1"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89609.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="355" mass="38875" checksum="D8C6C68462704948" modified="2012-04-18" version="2">MVIPEADGTLSFDPSLTRSQGRKLVKEFVDEARKHKVNPLISTGGWTGSRHFSNLTSSDYSRKRFAQILVNFSKKHGFTGIEMDWEYPNGEGIGCNSRRPADVINFGLLAKEIRTLWPEVELTAAVGVTGLIGATGEPATPKETAFLTENLDFINLMAYDVYGSWSDSTGPIAPIKGTCAGAGADQSVETGLQVALKQGFKASQILLGIPGYAKRFQLLSPKLLPKTVGKQTSFYYQKKSDVTPAGGKFDDKPGKDVCGNPQTWGGSFLVTELIKNGWLSKDQTRGLDGYKRVVDECSGQPFLANEKYLISYDDKESTLAKAKFAKQNKMGGVYFFDTMGPTDETVRATRKFLSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZD7</accession>
    <name>E3KZD7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Guanylate kinase</fullName>
            <ecNumber evidence="2">2.7.4.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">GMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_15625</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the guanylate kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.8" evidence="2"/>
    <dbReference type="EMBL" id="DS178324">
        <property type="protein sequence ID" value="EFP89662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334081.1">
        <property type="nucleotide sequence ID" value="XM_003334033.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZD7"/>
    <dbReference type="FunCoup" id="E3KZD7">
        <property type="interactions" value="362"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KZD7"/>
    <dbReference type="GeneID" id="10545228"/>
    <dbReference type="KEGG" id="pgr:PGTG_15625"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15625"/>
    <dbReference type="eggNOG" id="KOG0707">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001715_0_1_1"/>
    <dbReference type="InParanoid" id="E3KZD7"/>
    <dbReference type="OMA" id="EWAVVHG"/>
    <dbReference type="OrthoDB" id="6334211at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004385">
        <property type="term" value="F:GMP kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00071">
        <property type="entry name" value="GMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.63.10:FF:000002">
        <property type="entry name" value="Guanylate kinase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000776">
        <property type="entry name" value="Guanylate kinase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.63.10">
        <property type="entry name" value="Guanylate Kinase phosphate binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008145">
        <property type="entry name" value="GK/Ca_channel_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008144">
        <property type="entry name" value="Guanylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017665">
        <property type="entry name" value="Guanylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020590">
        <property type="entry name" value="Guanylate_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03263">
        <property type="entry name" value="guanyl_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23117:SF13">
        <property type="entry name" value="GUANYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23117">
        <property type="entry name" value="GUANYLATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00625">
        <property type="entry name" value="Guanylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00072">
        <property type="entry name" value="GuKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00856">
        <property type="entry name" value="GUANYLATE_KINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50052">
        <property type="entry name" value="GUANYLATE_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Guanylate kinase-like" evidence="9">
        <location>
            <begin position="49"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP89662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="252" mass="28048" checksum="D14D41C4662928A1" modified="2011-01-11" version="1">MYKHCLHQLIQPIHQRLTKPSISSFINTPTKHPIRTTSTSMAPSASAQLQPIVVFGPSGSGKSTLIKRLQASQDWPQFGFSVSHTTRKPREGEADGVAYHFVSRESFQEMVTKGEFIEHAEFGGNCYGTSLKAVSEVSEHQKKRCILDIDAQGVKLILKNHPSLSPLIIFVCPPSIEELRSRLEARGTESEESLKSRLGMAQSEIDYAKSGPSDLIIVNKNLDEAYRKFHLACTVNKNENPSQFPQSDPIPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3L134</accession>
    <name>E3L134_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16537</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178330">
        <property type="protein sequence ID" value="EFP90259.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334678.2">
        <property type="nucleotide sequence ID" value="XM_003334630.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L134"/>
    <dbReference type="GeneID" id="10546402"/>
    <dbReference type="KEGG" id="pgr:PGTG_16537"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16537"/>
    <dbReference type="HOGENOM" id="CLU_060386_0_0_1"/>
    <dbReference type="InParanoid" id="E3L134"/>
    <dbReference type="OrthoDB" id="2511556at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="142"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="212"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90259.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="331" mass="36348" checksum="AFF7D67431F7501D" modified="2012-04-18" version="2">MFFEYGFPQDRREIAPDSPERFHERAVVGLVEGHWLDACNQPINQKLMMDSSNRPVPANHILLCEPSPVDNINSCLPRAGTAFERGLESQPEHEALRSIAENGWKVVYKVMRARFGVKGFEVVYAFDDNHPFGLDLPVGHRPCTRLSGQPPRRKKVKTSASGGTSEVGIADLTLSQSHNVSNGATPPAPRAQNHSYSTLTPASGSNIIRQYSQSTHPSSTNPPSSRLVSPTRPKQSQWHSAQSLLTWWLAHNKLSTAHLSAAQNSITEFLSSLSPALTPYGTHFKWLGVQSPVDLLGLIDSGGEEELEVFLDEIDVLVGSVLGVKGPRMKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
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    <name>E3L237_PUCGT</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16638</name>
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    <organism evidence="2 3">
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178335">
        <property type="protein sequence ID" value="EFP90612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16638"/>
    <dbReference type="HOGENOM" id="CLU_144321_0_0_1"/>
    <dbReference type="InParanoid" id="E3L237"/>
    <dbReference type="OMA" id="PGHGYHH"/>
    <dbReference type="Proteomes" id="UP000008783">
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174100" evidence="1">
        <location>
            <begin position="23"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="117" mass="10529" checksum="CA1A2021678B2CDE" modified="2011-01-11" version="1">MKFTFGVLSIVATILSTPTVTAVPTHGFLGRHGGYGGGYGGGYGGGYGGGYGGGYGGGYGGYGGGYGGLGGGYGGYGRGYGGGYGGGYGGGYGGGYGGGGGGLGFGLGLGAGVGIGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3L284</accession>
    <name>E3L284_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16685</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178335">
        <property type="protein sequence ID" value="EFP90659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335078.1">
        <property type="nucleotide sequence ID" value="XM_003335030.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L284"/>
    <dbReference type="GeneID" id="10530559"/>
    <dbReference type="KEGG" id="pgr:PGTG_16685"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16685"/>
    <dbReference type="HOGENOM" id="CLU_181706_0_0_1"/>
    <dbReference type="InParanoid" id="E3L284"/>
    <dbReference type="OMA" id="MEAHDHG"/>
    <dbReference type="OrthoDB" id="2499436at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="102" mass="11199" checksum="CBB8993AD1C701B2" modified="2011-01-11" version="1">MQPSHDHTSNLTHTTQATDHHHDPGLTGEAGLNLQNNPTHPHDNVTVHPEGNINKAEKTETQVPFKAKVEGYAKKFAGKTFGKEHEVAAGEARLRGEDQPKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>E3L324</accession>
    <name>E3L324_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Enhancer of polycomb-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_17221</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair. Involved in gene silencing by neighboring heterochromatin, blockage of the silencing spreading along the chromosome, and required for cell cycle progression through G2/M.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the enhancer of polycomb family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178339">
        <property type="protein sequence ID" value="EFP90949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335368.1">
        <property type="nucleotide sequence ID" value="XM_003335320.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L324"/>
    <dbReference type="FunCoup" id="E3L324">
        <property type="interactions" value="345"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L324"/>
    <dbReference type="GeneID" id="10528363"/>
    <dbReference type="KEGG" id="pgr:PGTG_17221"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17221"/>
    <dbReference type="eggNOG" id="KOG2261">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_336162_0_0_1"/>
    <dbReference type="InParanoid" id="E3L324"/>
    <dbReference type="OMA" id="HKSASIQ"/>
    <dbReference type="OrthoDB" id="435275at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032777">
        <property type="term" value="C:piccolo histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024943">
        <property type="entry name" value="Enhancer_polycomb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019542">
        <property type="entry name" value="Enhancer_polycomb-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14898">
        <property type="entry name" value="ENHANCER OF POLYCOMB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10513">
        <property type="entry name" value="EPL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Enhancer of polycomb-like N-terminal" evidence="9">
        <location>
            <begin position="9"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="21"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="194"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="416"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="707"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="225"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="730"/>
            <end position="768"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU361124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP90949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="774" mass="86660" checksum="FE656F4EBE8A5652" modified="2011-01-11" version="1">MPKVSARFRNRKINFRTRITIHTGPLDTPSDHEEDDYPFEDDRRQSIGGPATDRSHIVETGVDKEEESELHLQKVINASTAALMRSAVNQRKPASPGLIAELADTAAHSTLATQSSTTINSNNNTAPNDSNVPHIPIPDAAGLVDPATYEALYAHSKIFLPSSYIRFSDTVEDTLGGVVYTMDEEDEAWLNRFNSENNAPHSDHNHKGGSTPDENGKNRRTNSRKGKEKERSVTETDGPGHLAEDDFEAIMDHFEKTTEETVPGLHLDTNRLPSLADLEPSLESPLISQRLSSVKIFAKFVYSHWKERRLKRGGKPIMPAVDFDETNESNPYVCFRRREIKMVRKTRRTDTQNMDRLVRLRNDLYKAQELLNTVLARERTKREAVELEKAIFEGRCLIREMKRSLNEADGDEELLVSKKEKRRKREQSGNGTLKSPLRNAAGPSLSGTGSAGASVLEDVQFYRDQARASTKLMERDYNRCREEQNGWEDLTDSAHLPMPLSAAALRWRGAVSSDPHLSSKSNREATVNFEQGLSTAPSVMEHNQFRKRVGRGGRLFLDRIVAPRQRRCTRAWLDAELAGDSEESARDRRLTDRWRYDDDLRQDFPSTDYPHIIDDYNVTYSARRSQLLDQDDLQYLDPPGEVYLAKVAEYYDRAPQPEPTIVKVGKLPSKVVPNLYGAGLGCPTFPSSGPAVTMPEQLLAAHASAQVPNLRLNPTGKRATPNGTGPAVNRTPTSTQASTRTRKNPSVNGSPTANANAKSSSLQGQPPIQQMVWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3L395</accession>
    <name>E3L395_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17292</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178340">
        <property type="protein sequence ID" value="EFP91020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335439.1">
        <property type="nucleotide sequence ID" value="XM_003335391.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L395"/>
    <dbReference type="GeneID" id="10528518"/>
    <dbReference type="KEGG" id="pgr:PGTG_17292"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17292"/>
    <dbReference type="HOGENOM" id="CLU_003292_6_0_1"/>
    <dbReference type="InParanoid" id="E3L395"/>
    <dbReference type="OMA" id="DWNMKEL"/>
    <dbReference type="OrthoDB" id="2508565at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="227" mass="24920" checksum="CA3F8DAD49522CE8" modified="2011-01-11" version="1">MENNKSTRSKTYARKEPVEAISSVAAENIATIANPTQTKVAEFSTARNSSDPHDSDEEGSVDLIAKIPETAREVILNTSIPPAKDLAAERSLVWTKLKEAQASGDKLLSKILLKAYNELEDTAIDGPPKMTRSISALPVLTNMSEELSIAKTIVNATETELEDNLVYAVGTVTNHQDIGFTPYFDDNIKKLKAPLPLTIFDREWQKKALTAHLMLKLSKNSEATKLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3D5</accession>
    <name>E3L3D5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16922</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178341">
        <property type="protein sequence ID" value="EFP91060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335479.1">
        <property type="nucleotide sequence ID" value="XM_003335431.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L3D5"/>
    <dbReference type="GeneID" id="10528692"/>
    <dbReference type="KEGG" id="pgr:PGTG_16922"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16922"/>
    <dbReference type="HOGENOM" id="CLU_003292_5_1_1"/>
    <dbReference type="InParanoid" id="E3L3D5"/>
    <dbReference type="OrthoDB" id="2503678at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052925">
        <property type="entry name" value="Phage_Integrase-like_Recomb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34605:SF3">
        <property type="entry name" value="P CELL-TYPE AGGLUTINATION PROTEIN MAP4-LIKE-RELATED"/>
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    </dbReference>
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        <property type="entry name" value="PHAGE_INTEGRASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
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    </dbReference>
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    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="24192" checksum="7F97F64B7E49C843" modified="2011-01-11" version="1">MLKHLIWLSNVMFPSGDIDKAILDLAIVAFWGMARIGELTYEKPFGDLNKQDSVLTSDVRLIKSAIGEKVILTIRNAKKAQPGKPQQIILHSLKNMLCPVLAIKRRMKAADGNDTSLFGFYRDRDRKHITRSIAVNRIQLVLRTGGFEGLLGHSFRVGGASLRYALGTPVEEICLLGRWISNCYRLYIREYSAEDTTESKRIIDELNELWKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3L4A6</accession>
    <name>E3L4A6_PUCGT</name>
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        <recommendedName>
            <fullName evidence="4">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178346">
        <property type="protein sequence ID" value="EFP91381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335800.1">
        <property type="nucleotide sequence ID" value="XM_003335752.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L4A6"/>
    <dbReference type="GeneID" id="10529294"/>
    <dbReference type="KEGG" id="pgr:PGTG_17337"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17337"/>
    <dbReference type="HOGENOM" id="CLU_058279_1_0_1"/>
    <dbReference type="InParanoid" id="E3L4A6"/>
    <dbReference type="OrthoDB" id="76215at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47072">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47072:SF4">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="267" mass="29309" checksum="9AFA266E8DFA456F" modified="2011-01-11" version="1">MTSKSPAINSKSLQESAASNGNANAPHTWMTLEKCKLLELLYDEMSAGHATDNGNLKKEGWTGFLCEQSGFGWDDDKFTVTADQRTWDELIQAHPRRNFGKLKDKPFPIFELAERVFVGNFATGESVNKHVSPDEVPVKVIDDSNAEAALTAPTPTSKKRKNNKKKEVVVSSLSNTDTDVRVLKKQSESTSTKRIRETKGTDGLVGAINNASNTIASLHKESSPSKDNQPNKTSSTGTTSTHDSLNAQALKCLSLYFLNKVDDEQYI</sequence>
</entry>
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    <accession>E3L4H0</accession>
    <name>E3L4H0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178347">
        <property type="protein sequence ID" value="EFP91445.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335864.2">
        <property type="nucleotide sequence ID" value="XM_003335816.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L4H0"/>
    <dbReference type="GeneID" id="10529447"/>
    <dbReference type="KEGG" id="pgr:PGTG_17695"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17695"/>
    <dbReference type="HOGENOM" id="CLU_1518591_0_0_1"/>
    <dbReference type="InParanoid" id="E3L4H0"/>
    <dbReference type="Proteomes" id="UP000008783">
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="125"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="60"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="126"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91445.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="177" mass="19749" checksum="4A40C8FF22608800" modified="2012-04-18" version="2">MSQQQQHTSYCPCLTYSMSRSSITKPILFAHLTNQLQVHSKPISSILSAQPHTLKPLPTSPHQSPYNPSSSQSSSEEPEEEIPEVEEEEQEAFASAVQSPAIGEVNDLPLHQNVLMETKQITSLTRLKSSPESSLSRPSIESSHNQQKTRLSNWQKNLESVICDQSNQLIFKASTHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3L4Y6</accession>
    <name>E3L4Y6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178349">
        <property type="protein sequence ID" value="EFP91611.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336030.2">
        <property type="nucleotide sequence ID" value="XM_003335982.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L4Y6"/>
    <dbReference type="GeneID" id="10531548"/>
    <dbReference type="KEGG" id="pgr:PGTG_17665"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17665"/>
    <dbReference type="HOGENOM" id="CLU_086121_0_0_1"/>
    <dbReference type="InParanoid" id="E3L4Y6"/>
    <dbReference type="OrthoDB" id="2500318at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172458" description="Secreted protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91611.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="214" mass="23445" checksum="420796353104E02A" modified="2012-04-18" version="2">MNRFSVFLCLALSIAAVKATEHSTCFNFFLKKDGCVNSSAANSTRCQAPPKDDPAPVQQFALVSQKAKRSEPHRVDRRYNSQNPVFPIGSGTGNCGLYDSAKELGVCLWSGAEQTNPTVQTAGWVNSLKTSNCGKRIYIHRTGKPDTVQYAKLVDGCSFNTTVLDIGCTQIGLSIQLFKKFNPSEKEQTDQLLYGGLTWDFDNLHGQSTQQAPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>E3L5Y1</accession>
    <name>E3L5Y1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_18407</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178355">
        <property type="protein sequence ID" value="EFP91956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336375.1">
        <property type="nucleotide sequence ID" value="XM_003336327.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L5Y1"/>
    <dbReference type="GeneID" id="10531908"/>
    <dbReference type="KEGG" id="pgr:PGTG_18407"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18407"/>
    <dbReference type="HOGENOM" id="CLU_2706036_0_0_1"/>
    <dbReference type="InParanoid" id="E3L5Y1"/>
    <dbReference type="OrthoDB" id="10392430at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP91956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="73" mass="8294" checksum="D27C2A8B6878B2E6" modified="2011-01-11" version="1">MPYVTAVLANPTQNQIQVQAKPFVSPPALVMTFWYSVHNHFPNPSKAGNESIFGGHDLNRSWSYHLVDLRRQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>E3L6D0</accession>
    <name>E3L6D0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="7">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_17410</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="7"/>
    <dbReference type="EMBL" id="DS178358">
        <property type="protein sequence ID" value="EFP92105.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336524.2">
        <property type="nucleotide sequence ID" value="XM_003336476.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L6D0"/>
    <dbReference type="STRING" id="418459.E3L6D0"/>
    <dbReference type="GeneID" id="10532065"/>
    <dbReference type="KEGG" id="pgr:PGTG_17410"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17410"/>
    <dbReference type="HOGENOM" id="CLU_011478_1_1_1"/>
    <dbReference type="InParanoid" id="E3L6D0"/>
    <dbReference type="OrthoDB" id="2507066at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="123"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="350"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP92105.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="721" mass="81273" checksum="47DE2C74A2BD74AB" modified="2012-04-18" version="2">MNRSETERSTPIADDSSHEEDSDSGQKTAASNPIEDDSSNEEDSNSGQRITESEVIDEIAALNRGESLPDELASLKLQATSKKRVTLRQDILELNHQELKEHIRVESRNLYGDEAKDEQLEAVAALVHDRHTFVLAGTGFGKTRIAEMYHNLFQPYQKSIVLVLNPLDSLGDNQVEEKKKVGVNNRHIKAVNLTSNVLDAKMARKIISGEFEFVYLSPEALLNNEIFRDVYFHPKFQSRLSLIVVDEAHMVYVWGLVANGQSKGLTSHLKHGERGVFRPGYGELAARLMATNGTPLLMMSATCRPIAINSILESFKLTRQMVTFVEAELTRPEIRMLRIDMQKSLASSEDLADLYSTQEQTPDNEIIPTLIYSTTRNLTGQVLDVVNDAREANQEDDPFSTFARRYHSITGDMDKSDVTGDFTKGVFPVVSSTMALGLGQNWKRVRCVIHMGRGDPASICQMLGRCGRDGKKGLAIMFVEPHRKNGKNSVSDFTNVELQNDDDRMDALAITPVCLRIAFSLDNRLGYIPLSFDDPNYKREAKREVDAGFSECSCSNCKPESSKWVINNFKQANINNFDSFISNSPQDIAELFPIRQEKQVADNQINWVEESGKKPLHEILEAFAQALVRYFCDFFDSEMKGAAAYPPDAYFSIIHARKISRNIKHLTLDNIEQLIGNEMLTGQFKMLFDHITLFQSTDSYQYISHRQEELDAELDSKAARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3L781</accession>
    <name>E3L781_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18231</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178364">
        <property type="protein sequence ID" value="EFP92406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336825.1">
        <property type="nucleotide sequence ID" value="XM_003336777.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L781"/>
    <dbReference type="GeneID" id="10538231"/>
    <dbReference type="KEGG" id="pgr:PGTG_18231"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18231"/>
    <dbReference type="HOGENOM" id="CLU_032241_1_0_1"/>
    <dbReference type="InParanoid" id="E3L781"/>
    <dbReference type="OMA" id="DEANYTR"/>
    <dbReference type="OrthoDB" id="2507256at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="381"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="392"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="411"/>
            <end position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="529" mass="56728" checksum="BB333139540B036C" modified="2011-01-11" version="1">MSISQLDPTLLTLSPLSDRLAPPPPGSQASGPDVTGLTLASDLSIVSGAQSPHLNTSAEVAGGKKKRYRRTQKEMAAFLSEQANIKKIKALEKAKEKRAKSRGRGGTCRLTQTSASEQSQANLNQGSAPVTSQPLVPDPNPPFNSDDYENVCSYLEEEANYTRLYGDGSKTSVGKNKVTKGAAYDMFAIFINSSSNLRLRLSGSQLRQRINGYKKQFMKAKDFAKNTGAGIEEGDGLPTLAELLEKKCPCYERMYAIFGVKANVTPLAQFDSGVGANLYADASNVRDGEEPTLSPEVFYSGWEESEVDQPPSGANTPAAGQDLNRCQSAIAHLTSLNIPNSDAEGDLDDDLLPPPLDFSGTAMDPSPSLMTQRLIARATQGSANPAGYSGASVPSVSQLTTRDPGGPSPANGVSTSRRAFANQQSTEASPAGPVREGNGKQKTTLASAFESSNTEKFAYLKAHMEWEKQKEDNRLQWEKEQYNKEAQRATEGAQGLAKLAESKLNAAQMWINQGKSSAEVDLLLKAIYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8L3</accession>
    <name>E3L8L3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">KRR1 small subunit processome component</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">KRR-R motif-containing protein 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_18806</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Required for 40S ribosome biogenesis. Involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the ribosomal small subunit (SSU) processome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
            <location evidence="1 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the KRR1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178375">
        <property type="protein sequence ID" value="EFP92888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337307.1">
        <property type="nucleotide sequence ID" value="XM_003337259.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L8L3"/>
    <dbReference type="FunCoup" id="E3L8L3">
        <property type="interactions" value="677"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L8L3"/>
    <dbReference type="GeneID" id="10542697"/>
    <dbReference type="KEGG" id="pgr:PGTG_18806"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18806"/>
    <dbReference type="HOGENOM" id="CLU_040185_0_2_1"/>
    <dbReference type="InParanoid" id="E3L8L3"/>
    <dbReference type="OMA" id="DMKHEDW"/>
    <dbReference type="OrthoDB" id="441223at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22393">
        <property type="entry name" value="KH-I_KRR1_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22394">
        <property type="entry name" value="KH-I_KRR1_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000011">
        <property type="entry name" value="KRR1 small subunit processome component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000014">
        <property type="entry name" value="KRR1 small subunit processome component"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041174">
        <property type="entry name" value="KRR1-like_KH1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048550">
        <property type="entry name" value="KRR1-like_KH1_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048548">
        <property type="entry name" value="KRR1-like_KH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048549">
        <property type="entry name" value="KRR1-like_KH2_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024166">
        <property type="entry name" value="rRNA_assembly_KRR1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12581">
        <property type="entry name" value="HIV-1 REV BINDING PROTEIN 2, 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12581:SF0">
        <property type="entry name" value="KRR1 SMALL SUBUNIT PROCESSOME COMPONENT HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17903">
        <property type="entry name" value="KH_KRR1_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21800">
        <property type="entry name" value="KH_KRR1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006515">
        <property type="entry name" value="KRR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 8" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 8" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="KRR1 small subunit processome component first KH" evidence="10">
        <location>
            <begin position="63"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="KRR1 small subunit processome component second KH" evidence="11">
        <location>
            <begin position="145"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="14"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="258"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="266"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="288"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="313"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP92888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="393" mass="45251" checksum="0F9179DE57AADAF7" modified="2011-01-11" version="1">MAIAKPVQDITTSNINEEVNEIQKSKKQKHRKEKPWDTDDIDHWKIEPFELEKEKIKPLTEESSFATLFPKYREVYLKEIWSHLTKVLEQHGVACVLNLVEGSMTVKTTRKTVDPYIILKARDLIKLLARSVPITQAVKILDDNVACDVIKIGNVIRNKERFVKRRQRILGPSGSTLKAIELLTDCYLLVQGTTVSAMGPYKGLKVVRRIVIDCMKNIHPIYHIKELMIKRELAKNPKLAEENWDRFLPNFTKRKRAKQAKAAKVSEQQPSVGRSVQDTSQAAAFRSEQAKQKSMQEKKKKKYTPFPPPQMPRKIDLELESGEYFKKKARTKSERADESKKAEGREIVSNLRKLNKAQDQPPAEESRANPVLTKKLNEKKRKFVPDDPAPDNH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8P3</accession>
    <name>E3L8P3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19047</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178376">
        <property type="protein sequence ID" value="EFP92929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337348.1">
        <property type="nucleotide sequence ID" value="XM_003337300.2"/>
    </dbReference>
    <dbReference type="GeneID" id="10542820"/>
    <dbReference type="KEGG" id="pgr:PGTG_19047"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19047"/>
    <dbReference type="eggNOG" id="ENOG502RDJB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_238027_0_0_1"/>
    <dbReference type="InParanoid" id="E3L8P3"/>
    <dbReference type="OMA" id="GFAYFQW"/>
    <dbReference type="OrthoDB" id="2507336at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216">
        <property type="entry name" value="PAXILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="358"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="481"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="602"/>
            <end position="653"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="668"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="791"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="831"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1060"/>
            <end position="1146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1171"/>
            <end position="1273"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1299"/>
            <end position="1443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1570"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1706"/>
            <end position="1726"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1745"/>
            <end position="1771"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1820"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1934"/>
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        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="31"/>
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="164"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="210"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="240"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <begin position="265"/>
            <end position="283"/>
        </location>
    </feature>
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            <begin position="300"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="358"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="407"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="429"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="620"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="686"/>
            <end position="697"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="803"/>
            <end position="818"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="862"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="880"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1087"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1104"/>
            <end position="1115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1185"/>
            <end position="1194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1259"/>
            <end position="1269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1328"/>
            <end position="1341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1366"/>
            <end position="1375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1432"/>
            <end position="1441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1585"/>
            <end position="1594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1745"/>
            <end position="1758"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1989" mass="217094" checksum="2B9ACA78A2140878" modified="2011-01-11" version="1">MLNNDRSNLSNSINSSSSSSSSFIPSNNNSPRLTGSEGSSSRHTNHTSSLLPTRTFSPTTTDRTSLPIDPLSPPIPNEHQLSPPSNHHHKRHQTTISQCSEQSSILFGTSQTDESPENNHTMPSPQHTDSQLQPSSSRRRLPSFNYTPDDESIEELLKTLGYSPGQSRANSLSSPNGPPDPNRSTQQRDNESRGSMVMLDIDGREFSIHEHSLSPVSERTELETESSKTSSNKRRPLVQSVLSQRFQSGLQSTAPLALSPKPKHLSTPSSPSSPRSSSPSSLRATVPKKIDHPSHFCQPVHQNRPASFGSHSPNSLSPVLTKSSHLLSSPPVPGNSLAHPSLHRLPKKTSHLIQLFENSKNQSPSSIPMSTPPNKTTPTNQIQSAPNSNLSSRFPSENHARGKSPQVPVSPTQTREGSNFYLTDHSAGRLNSPQSKAPQSPAAGSTESLSSAALPGPSMTEQECLRLRRERRERIAMASKNHAAASASRLKNESQTVHLAPPSPMSDHFLKHRDQLHRRSPRESVMFSGCTSVESVRSKRSYGPDFNEPHLHILRIAWAEFDPRSNSRTEFEEFLGCSRATQRADWVGAIWQAAHDLGGFVDQPDIPLRGNPSPKSDQLNNPAASSPSAANTHQPNTASPALPSKQDCSPRLDPVSLVDRSVSVGPAEAPQLAHSPTSQLKPPPSSAVSSRTSEVSTGTSNLQSELDRDLVAVQQATNPQILSPACRLYSEGNDPIVQENFNGVHVVDPAYQDYPINPNQEENYTRSRSPELDIFAMLDRMSVKGSIHRNTAEFYDPPKPESLQPQQVQDMHNPQPISNSLHANITALEDTANQSHRSPSRHSYHTIRPTSATGVHEQLPDSARSSAVGSRSSHREQLSEVRSMSQATHASQNRHLSVTPTPITQRPAGSRPQTTPKPRSSEGLHEPTTARQARGAWSDTDRLSNQTDNILLGANPARAVQRDLRRILKTIERNDSYRAAESDSLGHYLDSIQNQLQNVAIKLRGHQVEREPNYPVNESDEPTIADKVDYMLSLCNILLESQHKVSSAVEKNLEAHGVSISNRSVHTRSMRSTANPSRRSSHVPTEPGMPGPAPLTPRPDSSLDNDRVTSPDRLDSPPLPPLPDANGGEENIVIDEGPGNPSELPPQVAIQEAGLSRIENLLVELLSRVDESTQRQNKEPAPPVPDKDVGDGHSTRSRRSHANTDSQSETLDLDADVALWKSRGNMSDPAGPWNDEKRHPLGQSVSSHAPSVYPPTEAETPSSSDQPTPQVIPDYNLAYEAEDQVARRIADERARAARRLEGVSTPFGYRQSIGQGYSASVKPRVPSHLSQPGPTIPPNQDQHAEDPYLPSSQNDPRLSRPDLEQSHWSSTTPTAEEQDDSTPPPPPPPKDDHFVAPPPSQPALSVRAPSRVPSRAISHQSAARPLSVASNPPAPIPPPPQLSTEELRNVVHEALQHHSTEQAPVLEEVVTLLRTQDQVNQASVLNQAEVSRYLTQLNTHLEGVLHKKNDEILQIGENVGRLEKQLAVLLEQSQQISSVIPLMNATASTSAPPPEQAPTSIDQPEEALNRDLDAGPSAEPHTDVDPGPDGPIDVSVHPQEGDALDPPLEGGEPHVTLMSKKPSVGYRITGPRARKISFNNKGLKGPRMPSGLATVGKLWGGPVPAADRAARWGTGAGGSLKRVPTKSSHVSENPATVEAALSAVNPDDPVGQEALSQMDHPDDDRAGTIALGVSHILKFLRENSEKEELRRQKKEQEKAAQPQPAIPDEMEQRRAKIEELQLRREATLAEDKAKQMEAMMQAMRQQAIEHDRLLKKISEELEAKKSKTSEPENEEEERKKRYEEAMSDVNNVLTNVESGVMAHLEDFKAQMMKEMKQTFEKVGELREQKQQIQADIADMLSYMSKVRGGGPDPRWQHPIPVPSVHGGPEGSVISGHAGPAGPAGTNYPFLSPIPESYPSPHADFPDRDGTEVGVARSNIGFGPRSPSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8U6</accession>
    <name>E3L8U6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_18984</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178377">
        <property type="protein sequence ID" value="EFP92971.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337390.2">
        <property type="nucleotide sequence ID" value="XM_003337342.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L8U6"/>
    <dbReference type="GeneID" id="10542864"/>
    <dbReference type="KEGG" id="pgr:PGTG_18984"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18984"/>
    <dbReference type="HOGENOM" id="CLU_2098025_0_0_1"/>
    <dbReference type="InParanoid" id="E3L8U6"/>
    <dbReference type="OrthoDB" id="2513827at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP92971.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="116" mass="13184" checksum="A959967B73458DBE" modified="2012-04-18" version="1">MIWAGFPQQPAGQKAQILASHVGTCSCQYRVQISRTDDSNAVDLTAENGRADSKARCKEFGDFGTLGRLVSLETLYLKTEVLFVRLFTLYVFVDCKQNERQETFVHDCQQKKFIVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3L9F3</accession>
    <name>E3L9F3_PUCGT</name>
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        <submittedName>
            <fullName evidence="8">Phosphatidylserine decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_19138</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0057 (PMP3) family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178384">
        <property type="protein sequence ID" value="EFP93178.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337597.2">
        <property type="nucleotide sequence ID" value="XM_003337549.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L9F3"/>
    <dbReference type="GeneID" id="10543328"/>
    <dbReference type="KEGG" id="pgr:PGTG_19138"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19138"/>
    <dbReference type="eggNOG" id="KOG1773">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107649_0_3_1"/>
    <dbReference type="InParanoid" id="E3L9F3"/>
    <dbReference type="OrthoDB" id="2802411at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000612">
        <property type="entry name" value="PMP3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659">
        <property type="entry name" value="HYDROPHOBIC PROTEIN RCI2 LOW TEMPERATURE AND SALT RESPONSIVE PROTEIN LTI6 -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21659:SF40">
        <property type="entry name" value="PHOSPHATIDYLSERINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01679">
        <property type="entry name" value="Pmp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="75"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="8"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP93178.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="149" mass="16263" checksum="34BB5937205ADAEA" modified="2012-04-18" version="2">MIPDRPEPNTTSLNSAYPPSKPNPLPHSLHKKNQSTRTTQIRGTMVKGSDIILILVAVIFPPAAALFVTGCGCDLLINILLTILGYLPGHLHAFYLIYKRMQAEERYGQEGYKYVGNATYEPIGYEPPANHPPPIVHQASAPPAYGSAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3L9X3</accession>
    <name>E3L9X3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19081</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178390">
        <property type="protein sequence ID" value="EFP93348.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337767.2">
        <property type="nucleotide sequence ID" value="XM_003337719.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L9X3"/>
    <dbReference type="GeneID" id="10543724"/>
    <dbReference type="KEGG" id="pgr:PGTG_19081"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19081"/>
    <dbReference type="HOGENOM" id="CLU_044243_2_1_1"/>
    <dbReference type="InParanoid" id="E3L9X3"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93348.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="176" mass="20447" checksum="26AD905AF8023FEE" modified="2012-04-18" version="2">MLQIKKKTAEATSSPITNDSQNKTREEAMDLDDPLTSLEDTPKRPNSPEIVVLSKKEKIRLLIKEHVAIWTRFEKEKSSVATNELRQILHQAQDSQKVLQRMITKEELEGYVKGWNPWTAKRELFPPPPKKEGKKRSSTSKRAQQYDDPRVWADVFEIGQAWRAAYNQRSRKALPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3LA09</accession>
    <name>E3LA09_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Membrane-bound alpha-1,6-mannosyltransferase Initiation-specific</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_19402</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 32 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178391">
        <property type="protein sequence ID" value="EFP93384.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337803.2">
        <property type="nucleotide sequence ID" value="XM_003337755.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LA09"/>
    <dbReference type="FunCoup" id="E3LA09">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="GeneID" id="10543730"/>
    <dbReference type="KEGG" id="pgr:PGTG_19402"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19402"/>
    <dbReference type="HOGENOM" id="CLU_524898_0_0_1"/>
    <dbReference type="InParanoid" id="E3LA09"/>
    <dbReference type="OrthoDB" id="3647at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000030">
        <property type="term" value="F:mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051999">
        <property type="term" value="P:mannosyl-inositol phosphorylceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.20:FF:000011">
        <property type="entry name" value="Mannosyl phosphorylinositol ceramide synthase SUR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051706">
        <property type="entry name" value="Glycosyltransferase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007577">
        <property type="entry name" value="GlycoTrfase_DXD_sugar-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32385:SF15">
        <property type="entry name" value="INOSITOL PHOSPHOCERAMIDE MANNOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32385">
        <property type="entry name" value="MANNOSYL PHOSPHORYLINOSITOL CERAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04488">
        <property type="entry name" value="Gly_transf_sug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="343"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="479"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="482"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP93384.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="519" mass="59362" checksum="37BBB79564964F29" modified="2012-04-18" version="2">MWKLPTSRRLWAFILVLSILVVIFWQHIFLLARILSVYPLWPIYSSSLSLSLDKDGLDTTFEKYPVEQWSVKWPPRSANGSWPAPDSSLLRLSDGSLARLPDETYGGGVSYSEPDESDLVPPILHHILLGMDRRRMPKTWTASRNSCLALHPESANYTHHFWDDKSAEEFMRKHYSFFLRDFLDYRHNIQRADALRYFVLHHFGGIFFDMDLECRRSLGPLRRFAVVNINASPVGVSNGFMMAQAQHPFLEQLIKNLKYYDRSWLGVPYFDIMLSTGPMFLSTEHLRFPASQRSTQLRVLGGRQHRLHGSVVTPLFRHLGASSWHQGDAKLFLKIRALVYQPFFSLMLLCGFIGGSVGWLVWKKQTTKQGVDRSWSQSSSSAGRLILWRFGFGSRRGRRRTAEEYERIEMDLESPTGMMNNRRLSPILEDDEDLEAKSNSDLEHKQSILNFGRKRPAYNTLKSPTTPSVFMSETENLCHNASSSGGSCPSTSSDSDDDGSSAEAVVVLIDRHPSKWRSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3LA25</accession>
    <name>E3LA25_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetylglucosaminylphosphatidylinositol deacetylase</fullName>
            <ecNumber evidence="2">3.5.1.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_19418</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PIGL family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.89" evidence="2"/>
    <dbReference type="EMBL" id="DS178391">
        <property type="protein sequence ID" value="EFP93400.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337819.2">
        <property type="nucleotide sequence ID" value="XM_003337771.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LA25"/>
    <dbReference type="FunCoup" id="E3LA25">
        <property type="interactions" value="263"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3LA25"/>
    <dbReference type="GeneID" id="10537267"/>
    <dbReference type="KEGG" id="pgr:PGTG_19418"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19418"/>
    <dbReference type="HOGENOM" id="CLU_1062222_0_0_1"/>
    <dbReference type="InParanoid" id="E3LA25"/>
    <dbReference type="OrthoDB" id="440160at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000225">
        <property type="term" value="F:N-acetylglucosaminylphosphatidylinositol deacetylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10320">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003737">
        <property type="entry name" value="GlcNAc_PI_deacetylase-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024078">
        <property type="entry name" value="LmbE-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993:SF11">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02585">
        <property type="entry name" value="PIG-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102588">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP93400.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="262" mass="29616" checksum="E7DE1CF2AEDA6456" modified="2012-04-18" version="2">MSASQSKNRTNQEEEDIPPISPLLEPSYQYPSRDNQIREFDYGLRKHRTKFRLLVVFLTLWPLLCWTGIFLLFSDHPALFPSSLRGSKSTLFVVAHPDDECLFFAPSILATVQRAKSHGALLVMSSGNHYGQGGLRRKELLGSCKQLGIREDRCDVLDISICLPKKFAFVHHTDVDLDPKEVQSFSWESSSSNKASKVILQTSQIPIRCHPIAPILNGDCYKWSLVESKWKHTSHSCLDCFTIAGQSSINHKKLLDKSHVPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3LB11</accession>
    <name>E3LB11_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_19720</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178406">
        <property type="protein sequence ID" value="EFP93736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338155.1">
        <property type="nucleotide sequence ID" value="XM_003338107.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LB11"/>
    <dbReference type="GeneID" id="10535106"/>
    <dbReference type="KEGG" id="pgr:PGTG_19720"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19720"/>
    <dbReference type="HOGENOM" id="CLU_1074157_0_0_1"/>
    <dbReference type="InParanoid" id="E3LB11"/>
    <dbReference type="OrthoDB" id="10307986at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="222"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="189"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="13"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="222"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP93736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="259" mass="28968" checksum="4C33F9D8D9E88872" modified="2011-01-11" version="1">MSGIEPQPPINNPTPESNRSLNNPTPESNRSLNNGMGALTSLVENLCTNVTSAAVTTLIKQCEIFEGLDEEEEASLYLAAGIPPPGPDAMPGTLASYELQIRTIVVRTLDGLDPEAWEDDDQFRSAFLAILAAEQHWAAHRNGLWWHRVCEFRRLIISDRITAQGEEAPARNRIVYNRNEVKLAHHRAIWVAEQDIARSQQNIQRAERTILTERQMLRTIAEEERRERENADDVGPGPNIDPSTPVPTVDEPVSSEDHV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3NY03</accession>
    <name>E3NY03_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="3">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_20408</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="3"/>
    <dbReference type="EMBL" id="DS990004">
        <property type="protein sequence ID" value="EFP94452.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338871.2">
        <property type="nucleotide sequence ID" value="XM_003338823.2"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3NY03"/>
    <dbReference type="GeneID" id="10527744"/>
    <dbReference type="KEGG" id="pgr:PGTG_20408"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20408"/>
    <dbReference type="HOGENOM" id="CLU_003526_0_0_1"/>
    <dbReference type="InParanoid" id="E3NY03"/>
    <dbReference type="OrthoDB" id="10261556at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF145">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="5">
        <location>
            <begin position="1057"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1238"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1238"/>
            <end position="1260"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP94452.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1528" mass="170159" checksum="EE83F5B6F351F249" modified="2012-04-18" version="2">MSDSNQHITPIFNVPCQYKEPISITTSPNDHALKGHKSTTMVRVEMSPLPHNHLTTVIPAGNQIRLTKEFPVLGFNTYAKTLVCLDCHCGVPTKEVTGHLSSSPHKINNINPDAIIQAFKSINVKVPLDNKRTPPKHPIPGSRGSICGHAVQKDSSMSNHFSEIHKDESHLTVLGGWASNSKRTYVQHLSNSTIRYFPVQYSAPEPEAIPVTNTPPSSNHPNSTLLAWDQLASALDNLDILGHKESISITAQDNLTTPESCFMNCSGIYTHITACTNLLQCSIIELYKKVHYPKLLANWKPWVEKWLINCMTELHSVHDAVRMEALRESIDNPPSRGLIQPLNHQTTIIQYASIAAEFIIFTLQTAGQPLATHFWSDGDTELATSVKKAASLLPLIQSADLNSEQEFAINLNVLFWQSVGFSCSILKRSKVQSALEQFIALSMIKPDGSFCPPSELTQTIAALQYTIRVATIFVCLRATELFAENPPDGIDPHNPHALECASIRTFFQYFHDDKLISPFFTISNWMRLASSTEFDEDLPISTYWSNSEMTQLVIGPTTITIEAVQRALRIAISSINGVIHTSLHGALFPDFPQYTLMDMAENTSVGFNYLVASENMQIRHFKLLQDWMLNHSSFGVAPPAWALAVSFKDGQFWVNLDDVCSKKHAWDWLESADLILEHIYFIYRVGCGLPDCKTEEAATLIRNLHSAPRNIYWRENRFLFQTNRHNRPSQVYLTTDLSMHLHNYLAYIRPVQMLLLRALNQHDTANTMFSFLWVGSRQGTWDMAQFDQILQKQCFLAGLEKIGLCAWRQASVSIAHAHLAAKIPASLFSTEGGLVNPTNYSQYADANFLNASQAWQAYWGIESHLACQKPLLPVPDTLAPAKKAQQALAIFTQDPDASFRDDFQGEWLSCLFMIQIQQISLVDQTVKDFKKLKTVSFQHFEKGNCIDLLAQVIIATTNQAADPHFCNQLRSCLPTRIIIDEAHSFLEDGYRGYMPGVTALTQLSTQLILTTASLPHSQEDDLLRITFGLRRLITKRQPTFRPELNIEVLRSPAKWEQLSSVIQPLINSFLLGPKDRAMIFIENKNAVELVGPQIEAVIHHSDLPDDVAAAAVEKWISGDVKSIVATSGFGTGINYLHVRLVCIYGIPNRETANKAYQQLGRAGRDGHQAHIFFIPEVSDSHGGIDDFKKNLMNPALCPANTFAQHQDNLNWSCRNFPPFLRCVCCSRLSANPALHPSPLSLSQPSSIPDWSPDPSAPSNSYTSSCTVSAKATLGAHVGSGLAQSRKRRRLEDTDPLAHKLATSNILAAESSLSITSLARSAEQARLQSMPNNRVNSNQNNHDNSLISHELVNLQRFIRKMKGACGDCYVWNNTITPAHPRQCKPMHGKCLRCRAGKGWANHAHSGTEHTTKNCSSTSFSASISEGSKGAHGFNICTRCGLPAGSQDRFNFHPNGNVGPNCESGFANIAEAICWYVYRAQRTELGKWFPTAPLDSEPAFSKWIGITSKDVSPAPWPNGIKLLLRYEENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3NY72</accession>
    <name>E3NY72_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CxC6 like cysteine cluster associated with KDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20477</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS990046">
        <property type="protein sequence ID" value="EFP94521.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338940.2">
        <property type="nucleotide sequence ID" value="XM_003338892.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NY72"/>
    <dbReference type="STRING" id="418459.E3NY72"/>
    <dbReference type="GeneID" id="10527901"/>
    <dbReference type="KEGG" id="pgr:PGTG_20477"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20477"/>
    <dbReference type="eggNOG" id="ENOG502S1HF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_088670_0_0_1"/>
    <dbReference type="InParanoid" id="E3NY72"/>
    <dbReference type="OrthoDB" id="3055037at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040898">
        <property type="entry name" value="CxC6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18721">
        <property type="entry name" value="CxC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxC6 like cysteine cluster associated with KDZ" evidence="1">
        <location>
            <begin position="109"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP94521.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="224" mass="24549" checksum="444568888AC6187D" modified="2012-04-18" version="2" fragment="single">CSVFSIANLYNSTFMGDDAQQSFFPRLSVEVCKDGMDIDTLIFNHASRDKVLMVPSSGIDQVRYNEAKKQCSSWIAAEGTAHKNHLCGLCTCITYGEETNNHSVVRAVVTDGLTIGHWRCSASAAQLENLAKELGHPAPDGPCRRTLDTVRNRYCSFHLPFLEGICQAQPCIQPTLSNKKTCGRQSHLDAAKTFGARVKSNFLLHSMLNRPGSNCPSVKRVGGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3NYE2</accession>
    <name>E3NYE2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20547</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS990095">
        <property type="protein sequence ID" value="EFP94591.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003339007.2">
        <property type="nucleotide sequence ID" value="XM_003338959.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NYE2"/>
    <dbReference type="GeneID" id="10528049"/>
    <dbReference type="KEGG" id="pgr:PGTG_20547"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20547"/>
    <dbReference type="HOGENOM" id="CLU_031407_0_0_1"/>
    <dbReference type="InParanoid" id="E3NYE2"/>
    <dbReference type="OrthoDB" id="10355652at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP94591.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="655" mass="75117" checksum="D4233C93C7DBD635" modified="2012-04-18" version="2">MSLPYSTPDLEEGFRTPFSSSKNRPFASSSSFNWNPEPTVPPPKVNKGKSAENQPRDPLDEDYDSDNSTPQQRRDPPEPPVSGYGLHNTANRNEPTFNRQQQEPEQQTSSRIIKHFKFAYDGNNFMKFLTRFERAAKACKATDYDKALQIGQFMKTEELLTQLEAMDGYDTYNWATLQKEMIERWGEFDDITSYTTTNLVNLVEEFSREDEILSYHEFQMYLQIFSEILNELVRNKDLKKRRDASLLFISAFPQRIQNNIRQTLIKNGQLPTGLDGISLPPLWKHVTEVAEMQIRLENDYLERILTCKAPNCHPKAEQSIEDLVKEVASLKKQIMELRPPVIYKHSPPVAPLEPVVDFTEPEIDVFEPATDIMGPGTYYEEPKMGITETTLKEATTTVLPDLVTEWKAASVNLMDTATELLHTVIDIPEMEVDKLDIFQSTKTSEVLDQSVQIIETLDQPTRINKNYVQFLLTENLTEDLEFPKAPKILWIQNINKHKPKLFNKQLFKPYLDSDQLSQDINNTPQNLESEKNQNNSVLLATKKFKTMGSVSPSQILTLPISNSTIVEETFVIPPKPSPPDITTTRITILEEQNQLTPLVEDSISRLSHFGFSNKLFLLFFLLIKLLFQLPQKIPPDHFGQLYATFKPHRILVGVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>H6QP24</accession>
    <name>H6QP24_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Midasin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_20723</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Nuclear chaperone required for maturation and nuclear export of pre-60S ribosome subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the midasin family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EHS63138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890693.1">
        <property type="nucleotide sequence ID" value="XM_003890644.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="H6QP24">
        <property type="interactions" value="376"/>
    </dbReference>
    <dbReference type="STRING" id="418459.H6QP24"/>
    <dbReference type="GeneID" id="13541381"/>
    <dbReference type="KEGG" id="pgr:PGTG_20723"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20723"/>
    <dbReference type="eggNOG" id="KOG1808">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000050_0_2_1"/>
    <dbReference type="InParanoid" id="H6QP24"/>
    <dbReference type="OrthoDB" id="2501245at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000142">
        <property type="entry name" value="Midasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000712">
        <property type="entry name" value="Midasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002472">
        <property type="entry name" value="Midasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003767">
        <property type="entry name" value="Midasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000148">
        <property type="entry name" value="Midasin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040848">
        <property type="entry name" value="AAA_lid_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048617">
        <property type="entry name" value="MDN1_AAA_lid_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012099">
        <property type="entry name" value="Midasin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041190">
        <property type="entry name" value="Midasin_AAA_lid_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48103:SF2">
        <property type="entry name" value="MIDASIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48103">
        <property type="entry name" value="MIDASIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17865">
        <property type="entry name" value="AAA_lid_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17867">
        <property type="entry name" value="AAA_lid_7"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21108">
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    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
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    </evidence>
    <sequence length="5214" mass="581326" checksum="5F77B606C2BE75DF" modified="2012-04-18" version="1">[encoded data, 5214 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>H6QP80</accession>
    <name>H6QP80_PUCGT</name>
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        <submittedName>
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
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                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
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                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    </dbReference>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPB5</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <lineage>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
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                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
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                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
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    </dbReference>
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    </dbReference>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20672"/>
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    <dbReference type="InParanoid" id="H6QPB5"/>
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    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="82" mass="8771" checksum="CCCBDD50B5B042CA" modified="2012-04-18" version="1">MDPMEISRGFPGTLNLLGHTAKRKSLSSVTDQHGFKTEGRAHIQDSRESQLNVKLSLGLPELGGSPHSSTLTASPTGQNKCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPL4</accession>
    <name>H6QPL4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">OPT family small oligopeptide transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_20881</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EHS63899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890594.1">
        <property type="nucleotide sequence ID" value="XM_003890545.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPL4"/>
    <dbReference type="STRING" id="418459.H6QPL4"/>
    <dbReference type="GeneID" id="13543215"/>
    <dbReference type="KEGG" id="pgr:PGTG_20881"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20881"/>
    <dbReference type="eggNOG" id="KOG2262">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004965_6_2_1"/>
    <dbReference type="InParanoid" id="H6QPL4"/>
    <dbReference type="OrthoDB" id="9986677at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004648">
        <property type="entry name" value="Oligpept_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22601">
        <property type="entry name" value="ISP4 LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="84"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="109"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="193"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="259"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="348"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="423"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="476"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS63899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="519" mass="57442" checksum="822A9A916F961495" modified="2012-04-18" version="1">MTVDEEEAIPLVLAGRPRKASTSSTSSSLSILLEDEEQEVIGTSSQTETIPLRHRSRSPPPNPIVANVVSLEDDSKATTLSFRVFLLGSFLGCTGAAIGQIFFFKSNGIGFNIFLIILVSYPMGKALERLLPNATISLTRRWSFRLNPGPFTLKEHLLIGVLASGASGSSYAGDIVAVQDLYYHRDIGHVGGLLLVLSTQLIGFGLAGMTYSLLVRPTNMVWPSSLVVMTMYNTLHSRSSGMTSFRPPAEDRLLMAKKFRFFSIVFVLITIYQYSSALSDQQPKHVHASPGIGLLLFYSIPTEEFILSIYLSLLSMSYSDTGVGMFSLSFDWAVITSISLQTPFYSSCNYFAGLVFSMWILVPILWYYDFWNYQSFGDNAVSSRLFNSKYEKYDVGAVVKPDLTLDIEKYNQMGPIQLTPYFALYYGIGFAVLTSAITTVILFHSDDIRMAMNARTKASTDAHVEMMEKSYAKVPASWYIMIGSSMTIAPINVVVAYPLQIPVWGQAVDLALVLLLNTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPQ4</accession>
    <name>H6QPQ4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrotransposon Copia-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_20783</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EHS64080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890489.1">
        <property type="nucleotide sequence ID" value="XM_003890440.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPQ4"/>
    <dbReference type="GeneID" id="13542978"/>
    <dbReference type="KEGG" id="pgr:PGTG_20783"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20783"/>
    <dbReference type="HOGENOM" id="CLU_156730_0_0_1"/>
    <dbReference type="InParanoid" id="H6QPQ4"/>
    <dbReference type="OrthoDB" id="2505547at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="134" mass="15298" checksum="BD0A1213CD04A92C" modified="2012-04-18" version="1">MLTSTSSDITPCAKFLQQPSIYKQDVEQLTANGSNFDKWKRGLTQIVLLTLSHPNFFDKLDNYSKLSDQENTCLLFLIQIMIHDELASLVDQYTKGTEAYDAVQTNFQGTVRFRQMEPIDKLLKFRVSGPSTEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPU2</accession>
    <name>H6QPU2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_20939</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EHS64329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890485.1">
        <property type="nucleotide sequence ID" value="XM_003890436.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPU2"/>
    <dbReference type="GeneID" id="13543018"/>
    <dbReference type="KEGG" id="pgr:PGTG_20939"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20939"/>
    <dbReference type="HOGENOM" id="CLU_2759022_0_0_1"/>
    <dbReference type="InParanoid" id="H6QPU2"/>
    <dbReference type="OrthoDB" id="9986677at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="27"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS64329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="70" mass="7882" checksum="38E9675E99EC0AAD" modified="2012-04-18" version="1">MDPKTQTLNVTAYEAYSPTYLSAGNGIQYGFFFAMYTALIVHVALYYHKELAEGFRSMWKSASGREGCKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQ04</accession>
    <name>H6QQ04_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EHS64487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890401.1">
        <property type="nucleotide sequence ID" value="XM_003890352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQ04"/>
    <dbReference type="GeneID" id="13542647"/>
    <dbReference type="KEGG" id="pgr:PGTG_20950"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20950"/>
    <dbReference type="HOGENOM" id="CLU_624270_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQ04"/>
    <dbReference type="OrthoDB" id="2498923at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="453" mass="50964" checksum="53E20EEB1389B088" modified="2012-04-18" version="1">MFKDRHIQSPACWIPKFHIFLDSRARLPANFHSQVIIFAGFLLQPSRMGPLFQGQRTPTLAPRSRHSRPAPYRSRTSIVHPNLALEADLITQTESRPRLLSTPTTRSPTAQAGLEANPLASEDDSDSEVEDDLYPNTTAFVAGLELGIQARDVAAELSSMPLVRREATIFSTLASLLQRMERLENRPVLLTPALPVPPPVTSNLAIPDPTGTEPSFTFGRRFKASMDFIRDSARRFMLRPDLDAYTQGTGANAPNPEQGLLSLVMGHINNQDARFHEQHLPPGAIRDLLIGDNDVVRLVKGITKHVRNKARNILLTGILQPAGLNSNNTIPNIHELSRLLWKHLTRNPRRLTELQIDGEIDPTLKVRFAYLRMATISNYMDPNMRNVSQWDTIDAQLALNRREPADYTAAWRHLISERDHELFADSPNFEDLDLECALCPTEEEINEAMILIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRA7</accession>
    <name>H6QRA7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21428</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EHS63098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889959.1">
        <property type="nucleotide sequence ID" value="XM_003889910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRA7"/>
    <dbReference type="GeneID" id="13540520"/>
    <dbReference type="KEGG" id="pgr:PGTG_21428"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21428"/>
    <dbReference type="HOGENOM" id="CLU_040957_0_0_1"/>
    <dbReference type="InParanoid" id="H6QRA7"/>
    <dbReference type="OrthoDB" id="2505029at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="214" mass="23765" checksum="8BA87BD4D64714C0" modified="2012-04-18" version="1">MSALRAPNHPATLNGHFQPITDSIANLVRANQYGYVKTPSFFQCGGFNYEKNEDFEVELVTNTALNNTLMADSIYALSGKLIALNDGSPPILSYFQDTVIRIGATGPDQPDFTNKSTVTSLGMVISRREVASNVSDSGSHLEVIVAHCNWDGQERVHRRFNIKYIVPGTKNLVKTHTLYQVGREISIIGRLVDFHMEDHMAVVVVRSHNTSYLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRI1</accession>
    <name>H6QRI1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dynein heavy chain, cytoplasmic</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Dynein heavy chain, cytosolic</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_21460</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="15" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the dynein heavy chain family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EHS63265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889909.1">
        <property type="nucleotide sequence ID" value="XM_003889860.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRI1"/>
    <dbReference type="STRING" id="418459.H6QRI1"/>
    <dbReference type="GeneID" id="13540640"/>
    <dbReference type="KEGG" id="pgr:PGTG_21460"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21460"/>
    <dbReference type="eggNOG" id="KOG3595">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041873_0_0_1"/>
    <dbReference type="InParanoid" id="H6QRI1"/>
    <dbReference type="OrthoDB" id="2506608at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000235">
        <property type="term" value="C:astral microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005868">
        <property type="term" value="C:cytoplasmic dynein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045505">
        <property type="term" value="F:dynein intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051959">
        <property type="term" value="F:dynein light intermediate chain binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008569">
        <property type="term" value="F:minus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902850">
        <property type="term" value="P:microtubule cytoskeleton organization involved in mitosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030473">
        <property type="term" value="P:nuclear migration along microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000996">
        <property type="entry name" value="Cytoplasmic dynein heavy chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035699">
        <property type="entry name" value="AAA_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026983">
        <property type="entry name" value="DHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043157">
        <property type="entry name" value="Dynein_AAA1S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46532:SF4">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46532">
        <property type="entry name" value="MALE FERTILITY FACTOR KL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12774">
        <property type="entry name" value="AAA_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Dynein heavy chain hydrolytic ATP-binding dynein motor region" evidence="13">
        <location>
            <begin position="9"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF12774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EHS63265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="263" mass="29093" checksum="C190193383B9CAF2" modified="2012-04-18" version="1">MLMQLLLPGKTESVKALGVKLGQFVLVFCCDEIFDFQAMGRIFVGLCQVGAWGCFDEANRLEERILSAVSQQIQSIQQGLKAISVDPKAEVELVGKTLKINSHTVIFITMNLGYAGQSNLPENLKKLFRSMAITRPDRELIAQVMFFSQGFRTAKTLASKVVPFFSLQSKQPHYNFGLQALKAVLTSSGHLKCGRLQIKSSMAATSNITNSSNSGAEQKILIQSVTETIFPKLVADDVPLLTRYVNFHCEILVLPTKQSNLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRS4</accession>
    <name>H6QRS4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Transmembrane 9 superfamily member</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_21546</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the nonaspanin (TM9SF) (TC 9.A.2) family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EHS63371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889809.1">
        <property type="nucleotide sequence ID" value="XM_003889760.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRS4"/>
    <dbReference type="FunCoup" id="H6QRS4">
        <property type="interactions" value="443"/>
    </dbReference>
    <dbReference type="STRING" id="418459.H6QRS4"/>
    <dbReference type="GeneID" id="13543039"/>
    <dbReference type="KEGG" id="pgr:PGTG_21546"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21546"/>
    <dbReference type="eggNOG" id="KOG1278">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010714_4_1_1"/>
    <dbReference type="InParanoid" id="H6QRS4"/>
    <dbReference type="OrthoDB" id="1666796at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072657">
        <property type="term" value="P:protein localization to membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007034">
        <property type="term" value="P:vacuolar transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004240">
        <property type="entry name" value="EMP70"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766:SF111">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY MEMBER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10766">
        <property type="entry name" value="TRANSMEMBRANE 9 SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02990">
        <property type="entry name" value="EMP70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5007362149" description="Transmembrane 9 superfamily member" evidence="7">
        <location>
            <begin position="23"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="261"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="322"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="357"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="395"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="424"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="480"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="515"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="553"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="583"/>
            <end position="612"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS63371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="622" mass="69733" checksum="FB017242B0DF43D1" modified="2012-04-18" version="1">MTRRKELRNALIFFQLLAPVWSFYLPGTAPRDYNEGDLVPLLVNALTPQISAHSKLKSVISYDYYHPQLHFCQPPEGPASQRESFGSVLFGDRLYDSPFQIQMRKNETCKKLCDSSIPATDAGFVNQAIQDRYALNWLIDGLPAAELKRDDGSGETFYSIGFSLGQVQEPVPILHNHFNIFLEYHVRNGNFRVVGALVWPASLDASASRTCDMESGQPMRLSENEDNTVPYTYSVIWTESATPWATRWDHYLHIFDPKIHWFSLVNSIVIVAFLCVMVGMILMRTVARDIGRYNAIDQIDDVQEDFGWKLLHGEVFRAPERLMLLSVAIGSGAQIVAMATVTLVFALFGFLSPANRGSLSTVMIVTWTLFSYVAGHVSTRMYQTYGGLSFKQNMILTACLFPTILFSVLNVLNFFLVASGAAGAVPFGTMVAIIAMWFLISLPLALAGSIMASRKGPLPIPVRVNQIPRQIPPTVWYMRFWPSALMAGILPFGAGFIECYFLLSSLFGNKVYYAAGFLFLTFTVVGLTTATVTVLMCYFHLCQEDYRWHERAFVTGGASAFWLVAYGLLYATRLSLHGFTSIALYLGYLMLLALLDFLMTGSIGYVATFFFVKKIYSRVRID</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>H6QS22</accession>
    <name>H6QS22_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine aminopeptidase S33 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21599</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EHS63468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889679.1">
        <property type="nucleotide sequence ID" value="XM_003889630.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QS22"/>
    <dbReference type="STRING" id="418459.H6QS22"/>
    <dbReference type="GeneID" id="13542844"/>
    <dbReference type="KEGG" id="pgr:PGTG_21599"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21599"/>
    <dbReference type="eggNOG" id="KOG1455">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1195385_0_0_1"/>
    <dbReference type="InParanoid" id="H6QS22"/>
    <dbReference type="OrthoDB" id="10249433at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="232" mass="26538" checksum="45BEAF774A99235F" modified="2012-04-18" version="1">MRTTEARKMIPPADEQEAARKQFWEKYPPTSKWPIPKDQEWYWTQTPRPVDLLPIWKSVFANPVLERLGIRSNDDPTYGRYALPFSPHEKLIREDQRYTCWDGRVFLEDGRTDGGAVAGPVDHKTSHWVWYQVWWDEEASKRTGVELDLLYCHGIGEYGGSFAKNARTFLDAGHGRSTGFHVYLTNLDCLAHAVHVVLGDIIKRDLAAHRPQRPVIVAGQSMGGFTTVLYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>H6QS50</accession>
    <name>H6QS50_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21697</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EHS63523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889668.1">
        <property type="nucleotide sequence ID" value="XM_003889619.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QS50"/>
    <dbReference type="GeneID" id="13542847"/>
    <dbReference type="KEGG" id="pgr:PGTG_21697"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21697"/>
    <dbReference type="HOGENOM" id="CLU_156039_0_0_1"/>
    <dbReference type="InParanoid" id="H6QS50"/>
    <dbReference type="OrthoDB" id="10269571at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="95" mass="10306" checksum="2DAE9957EA12A351" modified="2012-04-18" version="1">MATPHLPPVSSPLAESGRPPTHPASFGANACKPKCYTPLPISLAPTIPMMTASGQHSYRITPCGIELMAHHEKFSLLFRRTRLRDIVGSSNTRPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTP4</accession>
    <name>H6QTP4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178319">
        <property type="protein sequence ID" value="EHS64259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889113.1">
        <property type="nucleotide sequence ID" value="XM_003889064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTP4"/>
    <dbReference type="GeneID" id="13541357"/>
    <dbReference type="KEGG" id="pgr:PGTG_22140"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22140"/>
    <dbReference type="HOGENOM" id="CLU_143669_0_0_1"/>
    <dbReference type="InParanoid" id="H6QTP4"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="111" mass="12728" checksum="5E8C6BD5BD8244EB" modified="2012-04-18" version="1">MRVKVSHPSGLAETHRRRLNGVSTRRRRAQYDEFLMLHPDASVVSENQENLSPPSPSYIGIQFDPSGIHSFLIQHKNLQDLRLITTNKRTNLESFLSSWLCQADCAWTLLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUC4</accession>
    <name>H6QUC4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">OTU domain-containing protein 1</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_22376</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Deubiquitinating enzyme that specifically hydrolyzes 'Lys-63'-linked polyubiquitin to monoubiquitin. Required for the stability and translation of a subset mRNAs with a high abundance of rare codons by mediating deubiquitination of 40S ribosomal protein RPS10/eS10, thereby antagonizing ZNF598-mediated 40S ubiquitination. The abundance of rare codons in mRNAs can limit the translation rate and can lead to ribosome collisions that trigger activation of ribosome quality control (RQC) pathway by ZNF598. OTUD1-mediated deubiquitination prevents activation of the RQC and subsequent dissociation of ribosomes and stimulates formation of polysomes and translation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="EMBL" id="DS178335">
        <property type="protein sequence ID" value="EHS64587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888894.1">
        <property type="nucleotide sequence ID" value="XM_003888845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUC4"/>
    <dbReference type="STRING" id="418459.H6QUC4"/>
    <dbReference type="GeneID" id="13542393"/>
    <dbReference type="KEGG" id="pgr:PGTG_22376"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22376"/>
    <dbReference type="eggNOG" id="KOG2605">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1010308_0_0_1"/>
    <dbReference type="InParanoid" id="H6QUC4"/>
    <dbReference type="OrthoDB" id="2502444at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd22744">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.80:FF:000010">
        <property type="entry name" value="OTU domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050704">
        <property type="entry name" value="Peptidase_C85-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419:SF10">
        <property type="entry name" value="DEUBIQUITINASE OTUD6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419">
        <property type="entry name" value="OTU DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="OTU" evidence="9">
        <location>
            <begin position="101"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00057633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00074858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS64587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="230" mass="26252" checksum="06D416DEEC3A3CA6" modified="2012-04-18" version="1">MEGRYSIKTRWTPTGNAEHSENFYKVDNMAQAGIYKSQKVASGSDSYLTDLTSSPSTSHELRVPTPSNVETLRTLANIRIITQRPASATIEPIKYLPGYSTTEHYIKKDGNCLFRTFAHFKYGDQEKHIQVRQEMIKYIEANPFEFQDFVEGEDPEPEIRLKKYLYRIKNGAWGDELSIKALSEAYELDVVVVSVSDRSMYAQLHVGNKGTTGFNGAYLQNDHYELLFQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>H6QV06</accession>
    <name>H6QV06_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_22615</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178367">
        <property type="protein sequence ID" value="EHS62619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888656.1">
        <property type="nucleotide sequence ID" value="XM_003888607.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QV06"/>
    <dbReference type="GeneID" id="13542268"/>
    <dbReference type="KEGG" id="pgr:PGTG_22615"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22615"/>
    <dbReference type="HOGENOM" id="CLU_026798_3_0_1"/>
    <dbReference type="InParanoid" id="H6QV06"/>
    <dbReference type="OrthoDB" id="2505311at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="141"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="61"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS62619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="376" mass="42401" checksum="9A79A997E707A804" modified="2012-04-18" version="1">MRSSKNKARQKRRKTSRDNEFETLISDQTMPGGVLIRGKRVLKFDLFPEISKELAIEKEKRMKEEQEFKENSQNRPDEKIQKPAHHPLTPRAPTEEENATALKLVEEKFHHANRDYGKIYNSANEQIIGVFEILKIPNLEQTQREDLDFLCAFIHDCKQFVSPAISEGLSCDDISSAIGWSKDLTRLEILPQYRNEESIRKNQEAYSKLMEASKKAGGILWDIFSTLGNLAAANTRAKMKKLASDGLVSSLAFPSNGFHTDYQVDNEEESDPPLSFAMMIPTFRSTGKIALESEGHRVDNGQFVFPDIKQAISFPPDTIGLMIFRAQQYAHGILSSTEVGDSTRLGLCIQAPVVINRGSQTEQDTPHDSEPEKAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>H6QV76</accession>
    <name>H6QV76_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22613</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178380">
        <property type="protein sequence ID" value="EHS62737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888588.1">
        <property type="nucleotide sequence ID" value="XM_003888539.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QV76"/>
    <dbReference type="FunCoup" id="H6QV76">
        <property type="interactions" value="161"/>
    </dbReference>
    <dbReference type="GeneID" id="13542351"/>
    <dbReference type="KEGG" id="pgr:PGTG_22613"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22613"/>
    <dbReference type="HOGENOM" id="CLU_2777159_0_0_1"/>
    <dbReference type="InParanoid" id="H6QV76"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS62737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="69" mass="7407" checksum="42392D4A024AFF94" modified="2012-04-18" version="1">MIRLHFGSISHCFIALDQPSSALVYFLKPSDAGYQAESLNGAIADGQRISVKAIKETKLGGLALLCRIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>H6QVN2</accession>
    <name>H6QVN2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_22803</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS989999">
        <property type="protein sequence ID" value="EHS63493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890868.1">
        <property type="nucleotide sequence ID" value="XM_003890819.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QVN2"/>
    <dbReference type="STRING" id="418459.H6QVN2"/>
    <dbReference type="GeneID" id="13541090"/>
    <dbReference type="KEGG" id="pgr:PGTG_22803"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22803"/>
    <dbReference type="HOGENOM" id="CLU_012390_7_2_1"/>
    <dbReference type="InParanoid" id="H6QVN2"/>
    <dbReference type="OrthoDB" id="2507429at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125">
        <property type="entry name" value="F21J9.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125:SF3">
        <property type="entry name" value="NO-APICAL-MERISTEM-ASSOCIATED CARBOXY-TERMINAL DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="2">
        <location>
            <begin position="130"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="132"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS63493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="262" mass="29256" checksum="81ECDF487C6FAC17" modified="2012-04-18" version="1">MAPVALDPLLQELGTQQTNSQPSQTAEIEASQPTEADKVGTKRSASYSESEDDGTTFWKRVHHSYSKNLPESCRTPGSLKAHWVALQKPISKFRGFVNQVEQFGESGALAEDCLNRALELFSKDQKVSFNKKNQLPVQKKRLRSPSSDAPASSALTSEPVSDAKASSTNTLSVQRPIGKKKAKLLHELAKREDSWKDIVARAHESVANESKRQNDIFNMEARSLDELAQNSKTNTQVSIMDKDLSNLDDDSKEYFRLKKKNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>H6QVQ2</accession>
    <name>H6QVQ2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22823</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS990075">
        <property type="protein sequence ID" value="EHS63789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890849.1">
        <property type="nucleotide sequence ID" value="XM_003890800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QVQ2"/>
    <dbReference type="STRING" id="418459.H6QVQ2"/>
    <dbReference type="GeneID" id="13541125"/>
    <dbReference type="KEGG" id="pgr:PGTG_22823"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22823"/>
    <dbReference type="HOGENOM" id="CLU_2360776_0_0_1"/>
    <dbReference type="InParanoid" id="H6QVQ2"/>
    <dbReference type="OrthoDB" id="10616463at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052072">
        <property type="entry name" value="Vascular_dev_regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16027:SF6">
        <property type="entry name" value="DILUTE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16027">
        <property type="entry name" value="DILUTE DOMAIN-CONTAINING PROTEIN YPR089W"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="96" mass="11176" checksum="4E35A28969264FA0" modified="2012-04-18" version="1">MASFHDEWEQEQWAEEFEWERCQPDQMLVCSLEELEGVFEAVIKTIKPRQARSDHSVPANALFFCARFATHMGTVELLEEVLLGAVERNRCIAAYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQ79</accession>
    <name>E3JQ79_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00325</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74369.2"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="154"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP74369.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="382" mass="44566" checksum="0C8B262DEC21AB2C" modified="2012-04-18" version="2">MSTPPGISHFSGKRPSYDMVNHQLGIGSSGFAESERSMESMKHQSFVQTNSIHNSQQMGDIAFNKTDPPPPPQPRKSINEILKFDTEVFEHNGHQSVADKSHAEALRMLIRDMQPGMDRLCFSADNPREVHGIFKSFSIKATRIRMDQKHHNRSTPAFQINSVGHSEANRKRKSGVALEEERRLLKVYARNRRKSLEERAVDKLFEHRFLWYRFWENRTGIKVVEQNLRLQKRVILFLFYTDMIGTILRKYMDNDSMKTAKFTARFLLQNALDIVSEHYRVSFQDPAQRAVSELPKEGKSVAAPAPLQAPKNPKLHRQELVWSWMRRLIDQLNNDSLRAIFLHNHGWTISPESQVFFNHVFYHSIEKLNSRLSIYYQQLHDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQ81</accession>
    <name>E3JQ81_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_00327</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178262">
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        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    <dbReference type="STRING" id="418459.E3JQ81"/>
    <dbReference type="GeneID" id="10528176"/>
    <dbReference type="KEGG" id="pgr:PGTG_00327"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00327"/>
    <dbReference type="eggNOG" id="KOG0114">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_25_2_1"/>
    <dbReference type="InParanoid" id="E3JQ81"/>
    <dbReference type="OMA" id="HQPDKMV"/>
    <dbReference type="OrthoDB" id="275748at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008380">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="RRM_SF3B14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000286">
        <property type="entry name" value="Putative pre-mRNA branch site protein p14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050441">
        <property type="entry name" value="RBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034150">
        <property type="entry name" value="SF3B6_RRM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48034">
        <property type="entry name" value="TRANSFORMER-2 SEX-DETERMINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="4" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="7">
        <location>
            <begin position="17"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP74371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="122" mass="14006" checksum="88522ED7D84BC6E9" modified="2011-01-11" version="1">MALARSHPVKLSGDVNRILFVKNLNYKTKGEDLYDLFGKYGAIRQIRIGTDPKTRGTAFVVYEDILDAKNAFDHLNGFHLQERYLVVLYHQFQRQAAKADLAKREAELIALKKTHNISDETE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQZ0</accession>
    <name>E3JQZ0_PUCGT</name>
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        <recommendedName>
            <fullName evidence="2">GmrSD restriction endonucleases N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="3 4">
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        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74323.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307329.2">
        <property type="nucleotide sequence ID" value="XM_003307281.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQZ0"/>
    <dbReference type="STRING" id="418459.E3JQZ0"/>
    <dbReference type="GeneID" id="10527970"/>
    <dbReference type="KEGG" id="pgr:PGTG_00279"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00279"/>
    <dbReference type="eggNOG" id="ENOG502S5H2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013023_2_2_1"/>
    <dbReference type="InParanoid" id="E3JQZ0"/>
    <dbReference type="OrthoDB" id="5419821at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="entry name" value="GmrSD_N"/>
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    <dbReference type="PANTHER" id="PTHR39639">
        <property type="entry name" value="CHROMOSOME 16, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39639:SF1">
        <property type="entry name" value="DUF262 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03235">
        <property type="entry name" value="GmrSD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GmrSD restriction endonucleases N-terminal" evidence="2">
        <location>
            <begin position="45"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="408"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="451"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="484"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP74323.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="633" mass="70599" checksum="41C8DF424C41424E" modified="2012-04-18" version="2">MPIGYVTDSDNDTDQLDGGASESDCDDKIIPGALQSANCRVWSIRELYSRMNNNTIDVAPPYQRDVVWTKPSQSALIDSILKNKWVPTLLFSERPACVDRHGKKRKARWVCMDGKQRLTSIKLFLDGVIPWYIKPKQPLFFKQSNPPQPSRKLLTEEQQDVFLSRGLTAAMYNQLTELQERDIFRLVQEGKPLTAGEKMHASSGPWGVYVDELTERYMVRNEDHPNGWCGRIANLTRGSDFKTMAQIVIMIRDRIYNSPDPPRFLTTVKVQKELDILSQTEVPDKLKREIEQVLDYFMDLSTLAPPMTPYTIVLGTPDALFYPIQKGKKTMIAPVEMVWIPYLIAVHAKELTRGKVLEMVELYKIDVRKKYPNEVKSNAPLTAWTANWIENFDLARLQGKYQGWFVPRPTPQPVFQDETKPEGSGLRDMARRNLSIQTAAKRDAPTPDPTLASSPCTSTASLPKRPRRSIPGPAGQGAAKSPTIPFQPNRNSLPKDSTPSMRPVAQTRSSTNSSAGIPPTSDALKARALAQRAQAVVAWSPNLMGNGQQSTASSLPSQTIAYHATDSGAVRPGALPQNGPRLVPNYPSRPSNFLLPNKPMSPNMSAIPQQQTNGNTHVLQSASTTHPNIPNWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3JR33</accession>
    <name>E3JR33_PUCGT</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00387</name>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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            <begin position="12"/>
            <end position="30"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="75"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="95"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP74431.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="141" mass="15544" checksum="408F7FE67EB9195F" modified="2012-04-18" version="2">MPDLPPVFFSWWLISIVALVCHIFGCLPVYAPQKLSQHRFRSEKLFSATLWILGLGWTLGFSAVLSLACFVEGFGDEYNLFSADFVYNQASAGNIFVLLTIAMVAQVIIGLAVIVQISNAPNKGTEEFSSSWTDHLRVTGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRW0</accession>
    <name>E3JRW0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NOT2/NOT3/NOT5 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_00597</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CNOT2/3/5 family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="GeneID" id="10527980"/>
    <dbReference type="KEGG" id="pgr:PGTG_00597"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00597"/>
    <dbReference type="eggNOG" id="KOG2151">
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    <dbReference type="HOGENOM" id="CLU_028051_1_0_1"/>
    <dbReference type="InParanoid" id="E3JRW0"/>
    <dbReference type="OrthoDB" id="25391at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030015">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0000932">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000289">
        <property type="term" value="P:nuclear-transcribed mRNA poly(A) tail shortening"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.1020:FF:000009">
        <property type="entry name" value="Related to CDC36-transcription factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1020">
        <property type="entry name" value="CCR4-NOT complex subunit 2/3/5, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038635">
        <property type="entry name" value="CCR4-NOT_su2/3/5_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040168">
        <property type="entry name" value="Not2/3/5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007282">
        <property type="entry name" value="NOT2/3/5_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23326">
        <property type="entry name" value="CCR4 NOT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04153">
        <property type="entry name" value="NOT2_3_5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NOT2/NOT3/NOT5 C-terminal" evidence="5">
        <location>
            <begin position="553"/>
            <end position="676"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="35"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="146"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="279"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="331"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="390"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="440"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="700"/>
            <end position="771"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="51"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="196"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
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            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
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            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="711"/>
            <end position="751"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007682"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP74641.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="771" mass="83484" checksum="CA3E00FA3C8AFC09" modified="2012-04-18" version="2">MSYCYMCMILCVDHQSTDAGTTSLVPPYTLCQSLRPPVPADKPPADHRTHTWSTTAQDSFDQTTVPYSQQAQPSQSTKPLSVSVSNHHQPPSPPGQQASTLAPTVTSSSTRSSFTWFSTHVMAFNPPGQQRQGLLTNKPGFSLSHNRNPIHQNSPTPGSIHPIQSKTTGPAGRGGAALGLNHFGNTNGMAVGHLPSSHSQSQQQLSNQQEASLDPSDFPALGAGAPSSTNPSHNALSYASHVNVSTANGLPSDRTVAATLAGQRTDFTPDDFPALDATVNQHHQQQQQQQQQQLSGSEGTVREAREQASAAAALTAQMARLNVAQNKTAGMVDSDNRNFPNKPPPGSNAAFHGSVVGSSHLSNGIGNLNSHHPLQSQAPAHLSLQSGAITSSNSHLHSTLRSPIPPNLTSMQPHRPTNLGNYPSNIGLANVPTTMIHTPASQNQSIQESGEANTGVENRSGGSDPSSQPTPLLDHAGSMDSISQNANCVSSVVPFFAPQTPAEQVLFGPADRFGLMNLLQIIRHADADYSMLAMGKDLTHLGLDLGSSEALYPKFMSPWSDAKQVASHAVEPEFTLPACYHVQPQPVQTKLPQFHEETLFFIFYSQPRDLMQELAALELYKKNWRYHKELQLWLTKESGTGPMEKTPHYERGFYVFFDPMIWKRVTKEFVLQYDQLEAKPALATNPAALQAATQLSLVYTSNGPHGKNSLANAANNNSQQQQQQQQQQQQQQQQQQQQQQQASQQQQNASNVHPAHHMMIGNNLPSNLVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3JSW2</accession>
    <name>E3JSW2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01730</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75137.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319556.2">
        <property type="nucleotide sequence ID" value="XM_003319508.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JSW2"/>
    <dbReference type="GeneID" id="10547579"/>
    <dbReference type="KEGG" id="pgr:PGTG_01730"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01730"/>
    <dbReference type="HOGENOM" id="CLU_032241_0_0_1"/>
    <dbReference type="InParanoid" id="E3JSW2"/>
    <dbReference type="OrthoDB" id="2507728at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75137.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="321" mass="34684" checksum="9B2A9FE689C98D18" modified="2012-04-18" version="2">MAPGRPIRPRVGSDGFVGVQARTPIRTWRAPGGLACAYQPPKEWAENTGAGIEEGDNLPTLAEILEKKCPCYDRMYSIFGGKANVTPLAQFDSGVGADLYMNADDTENPQDRESSPEQESRRGSVLHEELPDLTLPTLDGDLSLADDVLPPPLNFSGAALTPSPGLATPRFLAGVNVRSSGAALGPPHVQTSTPDSPHPGQGDGILAGRRSFANQRSTDASPAGPPRGVNPRARSTLASAFENSNSEKFVYLKEHMQWEKEKEENRLKWEKERYNKEAAVAKDGLQGQMKLAESKLKAAQEWISQGKSHTEVEGLLKAIYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3JTC9</accession>
    <name>E3JTC9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_01796</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319622.1">
        <property type="nucleotide sequence ID" value="XM_003319574.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JTC9"/>
    <dbReference type="GeneID" id="10542576"/>
    <dbReference type="KEGG" id="pgr:PGTG_01796"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01796"/>
    <dbReference type="HOGENOM" id="CLU_1251189_0_0_1"/>
    <dbReference type="InParanoid" id="E3JTC9"/>
    <dbReference type="OrthoDB" id="10278644at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172105" evidence="2">
        <location>
            <begin position="20"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP75203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="220" mass="23479" checksum="5A9FB0DA2F788D65" modified="2011-01-11" version="1">MNSFAILAACILLSLKVNANVHNTYTAQPQEHPSYSLAAAGYSPSYPSHAVLPTSNPTSFQGDNSQLLNAPDGYLKPVISRHAGSQPALAPGVYTKPDGTAHSVHPVPSLPEAVPGVIESKHNPVLYSSGEPKIARESLGLPDYTSSSHPFRGVQGGPGSQEYGTPVQSTRNVIAGRDLQTRTYCIIPARCRAPVHCAHPASCRALARYCWKKASRCGYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>E3JTT1</accession>
    <name>E3JTT1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_00744</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the UPL family. TOM1/PTR1 subfamily.</text>
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        <property type="protein sequence ID" value="EFP75413.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003319784.2"/>
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    <dbReference type="STRING" id="418459.E3JTT1"/>
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        <property type="term" value="F:ubiquitin protein ligase activity"/>
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        <property type="project" value="UniProtKB-UniPathway"/>
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        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
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        <property type="entry name" value="E3 ubiquitin-protein ligase NEDD4-like"/>
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        <property type="entry name" value="E3 ubiquitin-protein ligase UPL1"/>
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        <property type="entry name" value="Probable E3 ubiquitin-protein ligase HECTD2"/>
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        <property type="entry name" value="Uncharacterized protein"/>
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        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
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    </dbReference>
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        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
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        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
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        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
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        <property type="entry name" value="ARM-type_fold"/>
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        <property type="entry name" value="E3_Ub_ligase_DUF908"/>
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        <property type="entry name" value="E3_Ub_ligase_DUF913"/>
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        <property type="entry name" value="E3_ubiq-protein_ligase"/>
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        <property type="entry name" value="HECT_dom"/>
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        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
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        <property type="entry name" value="HUWE1/Rev1_UBM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254:SF67">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HUWE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11254">
        <property type="entry name" value="HECT DOMAIN UBIQUITIN-PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="UBM"/>
        <property type="match status" value="3"/>
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    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
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    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
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    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 7" id="KW-0833">Ubl conjugation pathway</keyword>
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        <location>
            <begin position="1427"/>
            <end position="1467"/>
        </location>
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    <feature type="domain" description="HECT" evidence="10">
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    <feature type="region of interest" description="Disordered" evidence="8">
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    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1106"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1493"/>
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    <feature type="region of interest" description="Disordered" evidence="8">
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    <feature type="region of interest" description="Disordered" evidence="8">
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    <feature type="region of interest" description="Disordered" evidence="8">
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            <begin position="2376"/>
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        <location>
            <position position="4141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00034494"/>
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    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
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    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
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        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
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        <source>
            <dbReference type="PROSITE" id="PS50237"/>
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    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP75413.2"/>
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    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="4174" mass="452381" checksum="99056DA2E377C139" modified="2012-04-18" version="2">[encoded data, 4174 characters]</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUG1</accession>
    <name>E3JUG1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01017</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003320057.1"/>
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    <dbReference type="AlphaFoldDB" id="E3JUG1"/>
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    <dbReference type="InParanoid" id="E3JUG1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="139"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="294"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="374"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="414"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="425"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="439" mass="48078" checksum="D9B9DEED0C8F034C" modified="2011-01-11" version="1">MGAHLRNGRNISIEQQQRELQRREQSRRETKSPPESGRGDRPAGLQRGDDQRYVFSPAAAERLRKLREQSAVDYRHRYEATGGRSIAVGARNFTRLNQFQSDFFSARDDVQPIDSGTSAHRPTNFSGQYEYRDGPAGFDSDPSSKPSDPVSSPGTASSPTGRRHDNGQHGVGNSNQPVQRSVEHVCPSPREQQPAIDESSSHPSHLQPRGDPTTSRRSGDAPHQRELDSSGRTSRSREVPTSQPDPTGEPLGDNSDSPRAHSQPVPDAAAPFDTPTLGRHVPGYWPHTARKQPYASTPATVYSTPLGHSQAEHRTTSASTQPADSSSIQRRGLLPTTTSSRDDGSEPSAAVPAANRSRPELRAPTAPPPSTATKLERIWEELETPPRPDEQVTRSRGLSHESGEDCGQGVQNSRQRESERKGERTTPSTRRTPSTEPPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWR6</accession>
    <name>E3JWR6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">FAD/NAD(P)-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_02932</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FMO family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76491.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EHS63913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320910.2">
        <property type="nucleotide sequence ID" value="XM_003320862.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890608.1">
        <property type="nucleotide sequence ID" value="XM_003890559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JWR6"/>
    <dbReference type="GeneID" id="10528264"/>
    <dbReference type="KEGG" id="pgr:PGTG_02932"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02932"/>
    <dbReference type="eggNOG" id="KOG1399">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024886_0_0_1"/>
    <dbReference type="InParanoid" id="E3JWR6"/>
    <dbReference type="OrthoDB" id="66881at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004499">
        <property type="term" value="F:N,N-dimethylaniline monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000884">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020946">
        <property type="entry name" value="Flavin_mOase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050346">
        <property type="entry name" value="FMO-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23023">
        <property type="entry name" value="DIMETHYLANILINE MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00743">
        <property type="entry name" value="FMO-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="96"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="23"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="62"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP76491.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="591" mass="64712" checksum="E46787E0BC14AFAE" modified="2012-04-18" version="2">MVHQIPNVASTTPTTSSALIGFLPPDRSPSSPSSLLSEQTTARSSSTSLFSLSSPRSTLSSIPDTSNSDQPPSSSSSSSSSSSGASALKGAFLGPLLNLLSSIWVVLYGLIQNVIIYLFAPSTDAITLEPSSNPLGRIAIVGAGITGISTAAHFLAHGFEVVIFEQSETIGGIWSRVNSTSSLQLDSLMYRFHPSVKWSNQFPVRKGEILSELQRIWNSYRLNSRTRFNFTVQQVTRQQASEDQPSKWTINNGAEGVFDGIVVAVGTCGPPERVNFQGREEFQGKMIHSSELDQIDWPGKRVVVIGGGASAVEALELAVQSGCATPAVLVTRTDKWFIPRNVIIGTLLAMNPFGIPCFIDQIAESAIRAYHYGADLKWMSPAAYNGKPADRLYSKTPIVNGEFLKLVRENRADYVRAKIHRISPQGVEVHRFGLDESEVVSADVIVEATGYKRPYLGFLPTRKLFRGDKTPTQFAPPNLFLQHFSANDWSCLMTNAGYYEGLGTVGHNHIGILARMMMMFMLDSKTAPTTAEMHAWVDRVVKLKGSLTFYTYSELSIWLATFLISNPRRWSWLAFVGMGWGGTGAIKMKTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWS4</accession>
    <name>E3JWS4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Neprosin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_02940</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320918.1">
        <property type="nucleotide sequence ID" value="XM_003320870.2"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="160054at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="entry name" value="SMALL SECRETED PROTEIN"/>
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        <property type="entry name" value="SMALL SECRETED PROTEIN"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173314" description="Neprosin domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <end position="291"/>
        </location>
    </feature>
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        <location>
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            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP76499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="291" mass="31707" checksum="0F3E4757FAD0228C" modified="2011-01-11" version="1">MGFTITTPMFLFILSLLAALVSANWDPSTGYLHNHRPSPAWLKKFKPSVSTTGIQVSECSINTRLHYPNVQLFAWFEVNHVWDGNHGCPYGTCHAYVVNPTSAEMVPSFTDGHSFFWHNIGGLRGNGVKPISNPKNGGYGYEDSLSGKFVDGPANPRSKQIGHDSNYPGFNKNKLHVWTPAQVDSFKGTDQSQKHPKCGRPCENNKDPGSSSGAYGGYKPASGSAYKPPKGWQPGKGDACFKNGNQSTPKSEESNQRQSSPTTNANKPKGNNSKSSSAPKHRKRALRNIGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXT5</accession>
    <name>E3JXT5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02321</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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    <dbReference type="GeneID" id="10529045"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02321"/>
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    <dbReference type="InParanoid" id="E3JXT5"/>
    <dbReference type="OMA" id="EACADEM"/>
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    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
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        </location>
    </feature>
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        </location>
    </feature>
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            <begin position="557"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP76860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3JY45</accession>
    <name>E3JY45_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Cytochrome c oxidase polypeptide VIIA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_02431</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003321341.2"/>
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    <dbReference type="AlphaFoldDB" id="E3JY45"/>
    <dbReference type="STRING" id="418459.E3JY45"/>
    <dbReference type="GeneID" id="10529237"/>
    <dbReference type="KEGG" id="pgr:PGTG_02431"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02431"/>
    <dbReference type="HOGENOM" id="CLU_1866145_0_0_1"/>
    <dbReference type="InParanoid" id="E3JY45"/>
    <dbReference type="OrthoDB" id="2317211at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
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    <dbReference type="GO" id="GO:0006123">
        <property type="term" value="P:mitochondrial electron transport, cytochrome c to oxygen"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="CDD" id="cd22888">
        <property type="entry name" value="CcO_VIIa_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28264:SF1">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 6C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28264">
        <property type="entry name" value="CYTOCHROME C OXIDASE SUBUNIT 7A"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
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    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="45"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP76970.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="137" mass="15455" checksum="9B6A4F756C0240E7" modified="2012-04-18" version="2">MSPFLSTKSFKKFYQIIMIRFRVGFHFIQRRLGHVAVGLLRRGSPTQQPSTTAINSSSINKPDITQTGSKTPSPKFVMAIAPITGKLRKRILLDLSVSLGLGTAAAYTWWYGYHVPKMRHQQNVYAKINSTRAEQES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZK6</accession>
    <name>E3JZK6_PUCGT</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_03437</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77481.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321900.2">
        <property type="nucleotide sequence ID" value="XM_003321852.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZK6"/>
    <dbReference type="GeneID" id="10538359"/>
    <dbReference type="KEGG" id="pgr:PGTG_03437"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03437"/>
    <dbReference type="HOGENOM" id="CLU_1787768_0_0_1"/>
    <dbReference type="InParanoid" id="E3JZK6"/>
    <dbReference type="OrthoDB" id="2508713at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP77481.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="148" mass="17279" checksum="94EC182FC56C245F" modified="2012-04-18" version="2">MEVAEQLTLDTSDLGDAVEQRIQRRGMSSTMKEDIEHPRLVNVAPLLGQPPQKPPIDVAECAYLLIHVARMDLQARVASSVLRRERERADLNINHAQVRIRLNELLQHISQEDKKLRDAHYDEFQRENQQEEEYVDSYFTLFYNSTPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3K142</accession>
    <name>E3K142_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein mS41</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_03973</name>
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    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS41 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP78017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322436.1">
        <property type="nucleotide sequence ID" value="XM_003322388.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K142"/>
    <dbReference type="STRING" id="418459.E3K142"/>
    <dbReference type="GeneID" id="10541326"/>
    <dbReference type="KEGG" id="pgr:PGTG_03973"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03973"/>
    <dbReference type="eggNOG" id="ENOG502S7YM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_126679_0_0_1"/>
    <dbReference type="InParanoid" id="E3K142"/>
    <dbReference type="OMA" id="WGPAVQN"/>
    <dbReference type="OrthoDB" id="18595at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039603">
        <property type="entry name" value="Ribosomal_mS41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019083">
        <property type="entry name" value="SAM_Ribosomal_mS41"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28235">
        <property type="entry name" value="PROTEIN FYV4, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28235:SF1">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09597">
        <property type="entry name" value="SAM_Ribosomal_mS41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="72"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="162"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="166"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF09597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP78017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="181" mass="20481" checksum="93052A0B1870F336" modified="2011-01-11" version="1">MFRSRLLLNQLNLNLNRTTTASYYSTTTTTTTAAAATTAATAATSTTKQTTSKPTQQTPKFARFVHKPSPPRIPKPHHGIETVEAFLESLRRPSLLSLTNKFTEWDQLFSLEPKTDLVKDGTLSVPKERRYLLRSMELFRQGLDPKEFAVGVRKPKKFRGWGPRVQHGKRLRGRPAGRMTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3K1K0</accession>
    <name>E3K1K0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_04131</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP78175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322594.1">
        <property type="nucleotide sequence ID" value="XM_003322546.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K1K0"/>
    <dbReference type="STRING" id="418459.E3K1K0"/>
    <dbReference type="GeneID" id="10541805"/>
    <dbReference type="KEGG" id="pgr:PGTG_04131"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04131"/>
    <dbReference type="HOGENOM" id="CLU_004591_1_2_1"/>
    <dbReference type="InParanoid" id="E3K1K0"/>
    <dbReference type="OMA" id="TYQLHFL"/>
    <dbReference type="OrthoDB" id="2246127at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP78175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="821" mass="94621" checksum="01A706D116EC0827" modified="2011-01-11" version="1">MAEPGGNQDLEEADGEGDRTLGDVGWFPFREKTYLVGMLLMGHMHNLMSVQDFDKVRTLWRHFGTTIPHWTTLRRARATIRSMLNMNMIYRLSLFDNKCYSLSLQQTIAMDMSNPIVNKHLDFYPEDTYGFNIYKLSQSLKWREDFPADLRVQMVEVQSKHYYIYEPIELEDKLIVIPVYFYMFQGELYSKCVKPKTRVTRDKNNQPHNYLIIPQDLPFDSPDLMVIPCSRFAKIYSEIFMLGGVPCSTWCENAIWGTKMGKSKKIELPNPWRAKAKGRIIRHVPITLYADDTSGNRSKQWNKHISYYYTLSGLPPNMTNQQYNCHFLATSNTAGALELADQIVDELNVLATEGFNAYDAGLKEDVLVMTMVLCFLGDSPMHAEIANTPMPSVSLNPCRMCDLSAPCKDSKSTLEYVNNFLGKDHEGRMIPFNLRKWEDTITNTHALWKIGKTRSKKLFDSRSIEYGVRDGINRACLMIIKDRKASKTKKEQINQMHKDNSPKLFSPILRLKGFDGCKDTPVEILHVVLLGVVKYLLRDFMMTTIKPKHLPQLIASWDSFIVQPLELGQIQGKYFVKHYKSLVGKHFRIVMQMAPFVLFQFMTEEQRRLWTALCQLTPLVFQTSIPDMAKYLNTLKDRLDLFLYLLIQMSAQWVNKPKFHMLLHLPDSIKRFGPASLVATEKFESFNGILRNASVHSNRHCPGRDLAISFANYECMRALGSGAKLFNHIDNRYFYASPQVTSMFRDPMIQKSMGFNDRLINTSPTITATYMSKKAEKTAAYSIPPHLKEAYPNKTIKPVESLKLDPHKVLTRNTFVQVKQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K1Q8</accession>
    <name>E3K1Q8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04189</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP78233.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322652.2">
        <property type="nucleotide sequence ID" value="XM_003322604.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K1Q8"/>
    <dbReference type="GeneID" id="10542025"/>
    <dbReference type="KEGG" id="pgr:PGTG_04189"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04189"/>
    <dbReference type="HOGENOM" id="CLU_160170_0_0_1"/>
    <dbReference type="InParanoid" id="E3K1Q8"/>
    <dbReference type="OrthoDB" id="10416369at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78233.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="129" mass="14845" checksum="543FEB88DBA5B4F6" modified="2012-04-18" version="2">MEIDEVPSQSPPASEQSPSPEAAPRRTLATKTLEICNHLKSNYNMTPKQFIEAFLTADSDALSFRRRYWGSKDGWPSTLDIIQKIKKQVKKTQKGQARWCEFIKNEVDVFKVARKLNHLSLTMMLYSPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K1Y4</accession>
    <name>E3K1Y4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04309</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78353.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322772.2">
        <property type="nucleotide sequence ID" value="XM_003322724.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K1Y4"/>
    <dbReference type="GeneID" id="10542307"/>
    <dbReference type="KEGG" id="pgr:PGTG_04309"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04309"/>
    <dbReference type="HOGENOM" id="CLU_355702_0_0_1"/>
    <dbReference type="InParanoid" id="E3K1Y4"/>
    <dbReference type="OrthoDB" id="2497898at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="726"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="737"/>
            <end position="755"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78353.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="849" mass="97037" checksum="2FFCB3D016CDF0F9" modified="2012-04-18" version="2">MDVLITNPRILLKPPDHPTNQLIKATQELGQQAIQMSGQAEEASTSRGIYQDMSGHMKEEDRASTFTIDPQYHSMKFSELKEEQLDAVCFNGQYGKAHILQLQKLKSMIEGIDKAKKSWQDIYLKSYGSKEKFDEDRAKTEHLSDVIRGILADVYDKRNANREEYEWCQRKLFDICPGMEEPGSHQSSFIGSAKADRSSKPGSHQLGYPLRRIRVCLRNRSATKQERILLDLGRAEQAGFTFSQEVISLAEKIAGLAQLIVDPANNVLLSSGERKLLKRLESHTLSLRRQDHLIRMGDRFQELQKQDTLLGLTDPDDLGLILETLKELHSRERNSPFWTDEDEKLAKALKMISKDCGTNQWKSQQIVTEIRNQKAYKVLRGKGVATLTLEDVQLCRKLSKNLQNYHKSYQLDKVDELTEEETIKVKDLAKQYYWMKEKFWEPKNYDPIGTAIKAMENEVSSVEELSLRFLRTKETLDSSQKILSKKLERGIPIIRLQEDTATNTERGVYENEMKLPDNRKIKRVKKALQSADDLRLIERYTEADLARLFEETVYSTPLSEFWIWGNQRHKPSIIYPELHSLLSLAESSVLSFEERCKVVIYSGKIKAKLYWEADAPPVTVESLALKRLKFLHSQNMRTLDAHENLIYASLNGGLFGEIWHLKSKLKIKSLALEELLVQACKPTIEDQKLALKLSGPNVFLSELNHSEWATIVYLLPDEIMKLGRLCSTPSPEDANGKDTTQPHNIPSSNLQSSPSDPLLEKNDHASFFKNDRFSQTLVLIQNASDRMNLKPKATTFEDSNPAAFLLEESEKEASNLAKYSTYAAKILDLHAKLKSKVARTKISESGRTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3K265</accession>
    <name>E3K265_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_04390</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
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    </reference>
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        <text evidence="10">Plays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28.</text>
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        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04390"/>
    <dbReference type="eggNOG" id="ENOG502RZMJ">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PI31 proteasome regulator N-terminal" evidence="13">
        <location>
            <begin position="46"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="PI31 proteasome regulator C-terminal" evidence="12">
        <location>
            <begin position="232"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
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            <begin position="186"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
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            <begin position="328"/>
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    </feature>
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            <begin position="1"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
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            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
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        </location>
    </feature>
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            <begin position="328"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00006405"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00024805"/>
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    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF08577"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF11566"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP78434.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="371" mass="39900" checksum="ED3E008B1EEBB26B" modified="2011-01-11" version="1">MTQLQTANSSKQEQQQQHQNNQPADLSSIYRSLQQELLTNKQVVEENIPNPLSLIALITHTILRSHSFTIKNSSDNQLQSILILNNQTHSSLLSYSHPRSSLSFEFTLSKIGNRISIAGIAVEDDRLSTLDLSISEYLDPAEFPLTLTKEHQTSSIGLRSTTHLLDFLSLFVNKILNHLLPDLIPPTSPNHRPPPPASSSTSTSQPSQPAPERFESPQMNLQSSHHRSPLEIGRSDLDPIGTSNLHIPSLYNAPSGGEGLLGGHSGMLVGGNHPIFNRQHQAPGRIGGGGGLPGASLPPGAVPPGARFDPFGPAFPATQPGFGPDFDPMGGLGQGLPLQNPHHGFSSARPDFNNFRPSGGQGGSGYDDMFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04482"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="82"/>
            <end position="91"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78526.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="245" mass="27391" checksum="0743FFAE1D0B6214" modified="2012-04-18" version="2">MNINQVFILAIAGLSLFAYPTLGALTSAKSAQKAASTASEGDKAVGDFGDIGRSTNLLSRDPPLDSRQSLNGERNNLEIAQAASNTRTQPTPALAARPGENQNEETEALKIVQEKRIHIAANDILKALNDVKDLEKQSSQGTLSRILSHLKKILMRPWKKFIDKRLRKAIALILRNQPDWLQRWAHEVASYHQVDLEHRVVEFNKAIADKLRNSAKLIEKYHPELMATYKENHGNKYLKGAGIHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3K389</accession>
    <name>E3K389_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Translation initiation factor eIF-5</fullName>
        </submittedName>
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        <name evidence="8" type="ORF">PGTG_04902</name>
    </gene>
    <organism evidence="8 9">
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        <lineage>
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        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
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                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
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                <person name="Zhong S."/>
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                <person name="Martin F."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
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    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="PROSITE" id="PS51363"/>
        </source>
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        <source>
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    </evidence>
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        <source>
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</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K393</accession>
    <name>E3K393_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04906</name>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04906"/>
    <dbReference type="HOGENOM" id="CLU_2122256_0_0_1"/>
    <dbReference type="InParanoid" id="E3K393"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
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        <location>
            <begin position="44"/>
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    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78950.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="114" mass="12276" checksum="4D55C4B3B16A3682" modified="2012-04-18" version="2">MPLNAPQGFSGLLNASQGPRSTAKRPRTPQCAPRILKTPPSAPQGPQTTCQSAPEPSRGPSEHPAASSKPQNPKKRGVEDLQNPKPPCKTPDQPPRTQRRIPRPSEPSEPQNPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3H5</accession>
    <name>E3K3H5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoserine transaminase</fullName>
            <ecNumber evidence="4">2.6.1.52</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_04988</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>4-(phosphooxy)-L-threonine + 2-oxoglutarate = (R)-3-hydroxy-2-oxo-4-phosphooxybutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:16573"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58452"/>
            <dbReference type="ChEBI" id="CHEBI:58538"/>
            <dbReference type="EC" id="2.6.1.52"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>O-phospho-L-serine + 2-oxoglutarate = 3-phosphooxypyruvate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:14329"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:18110"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57524"/>
            <dbReference type="EC" id="2.6.1.52"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.52" evidence="4"/>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP79032.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323451.2">
        <property type="nucleotide sequence ID" value="XM_003323403.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3H5"/>
    <dbReference type="FunCoup" id="E3K3H5">
        <property type="interactions" value="397"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K3H5"/>
    <dbReference type="GeneID" id="10530853"/>
    <dbReference type="KEGG" id="pgr:PGTG_04988"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04988"/>
    <dbReference type="eggNOG" id="KOG2790">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034866_0_0_1"/>
    <dbReference type="InParanoid" id="E3K3H5"/>
    <dbReference type="OrthoDB" id="1703350at2759"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00197"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004648">
        <property type="term" value="F:O-phospho-L-serine:2-oxoglutarate aminotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000010">
        <property type="entry name" value="Phosphoserine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000006">
        <property type="entry name" value="Phosphoserine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00160">
        <property type="entry name" value="SerC_aminotrans_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020578">
        <property type="entry name" value="Aminotrans_V_PyrdxlP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022278">
        <property type="entry name" value="Pser_aminoTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003764">
        <property type="entry name" value="PRK05355.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43247">
        <property type="entry name" value="PHOSPHOSERINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43247:SF1">
        <property type="entry name" value="PHOSPHOSERINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000525">
        <property type="entry name" value="SerC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00595">
        <property type="entry name" value="AA_TRANSFER_CLASS_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0718">Serine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="13">
        <location>
            <begin position="13"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EFP79032.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="413" mass="45805" checksum="73893B716EF8E38B" modified="2012-04-18" version="2">MSPSSLRDRTINLGAGPCTLPTSILEVASQGLLDYEGTGMGLVELSHRSKDFQKLNSNTIDNLRTLLTVPENFEILFMQGGGLTQFSCVVMNLVNQFRLKNKSDPSDQVVVDYLITGSWSLKASKEAARLGCQVNVVADGRQFSADSKSFHSIPNPIDWKLSQQLDQPKSTPAFLYYCDNETVHGVEFQTPGFPIDQLPEAYQEKVPLVADVSSNILSRKISPQLWKRLGIVFAGAQKNMAPSGLTVVIVRKDLITNLDEAVPFGGLRVPDMLSYKNMADHQSLYNTPPMFSIYVCNLVLKDLLSKGGVEAIEEINKEKSKLVYQVIDNSQGFYINSIDQNARSRINIIFNCRGGPSIEDQFALEAEKSYGIKQIKGHRSIGGIRASLYNAVTLDQVKVLCNFMREFMSKNST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3K492</accession>
    <name>E3K492_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CBM1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05423</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79102.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323521.2">
        <property type="nucleotide sequence ID" value="XM_003323473.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K492"/>
    <dbReference type="GeneID" id="10530925"/>
    <dbReference type="KEGG" id="pgr:PGTG_05423"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05423"/>
    <dbReference type="HOGENOM" id="CLU_2360775_0_0_1"/>
    <dbReference type="InParanoid" id="E3K492"/>
    <dbReference type="OrthoDB" id="2507909at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173526" description="CBM1 domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79102.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="96" mass="10397" checksum="99B5A78A333A52FD" modified="2012-04-18" version="2">MNVIKFALLAFVFGVSNVRSKNLKPCGGNTPAYACADDPSRSPDPLIYIPPTDLDCTLTPSEVPWCCPQGAISPNHPSKLGYAIDHGCQKRQPRVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3K4U1</accession>
    <name>E3K4U1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_05577</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79256.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323675.2">
        <property type="nucleotide sequence ID" value="XM_003323627.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K4U1"/>
    <dbReference type="GeneID" id="10531080"/>
    <dbReference type="KEGG" id="pgr:PGTG_05577"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05577"/>
    <dbReference type="HOGENOM" id="CLU_040573_0_0_1"/>
    <dbReference type="InParanoid" id="E3K4U1"/>
    <dbReference type="OrthoDB" id="2499170at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040223">
        <property type="entry name" value="PAR_bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988:SF27">
        <property type="entry name" value="GH27708P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988">
        <property type="entry name" value="THYROTROPH EMBRYONIC FACTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP79256.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="505" mass="56824" checksum="1E409ABFB7BC2A1B" modified="2012-04-18" version="2">MAKLSDLPSELVYRIIDYILVRDDQVPDDWQGQPIDADHHHLLDHNGIGPIKPKPRPHLERFSRNPTPRTLPRHFRAAATSQPNASWPGAIPSNPLLPLSLVNRTFRRCAQELLFKNVPVLSRWRAISFLQTLTWHAIQNPSTPAGGWFNRKQKKNAQDNNVWMVRWFDIDYSRLSSLARHVRSLQLVCEGLCSVVKGSGHLFCGIIRSCPLLTNIAISTAVSMDCKDYMLEALSSRPLIKEFVILENLDHSRSIFQWNVDDVVGRLFSQWDHLETVELSGLRGPSGQLMGSAQRPIPILNCDVLTLILRNPELDEQELSRFLKCFRHSMRTLEISNPGGGIDVEGFGRILQGSTNPDLECLKVENDWRLDDSESIVSSAVGDVDHDIPHSNKSLLDGVFESPSALRRLKSLSFNCDTATNKLFQCLPDSIVKLAWEDCDISLTAFAEVLSSSTLDKPVLPNLKCCSVRHRWGLSPEEFRNAQAALEIRDACFHSDFTLEDGSPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3K577</accession>
    <name>E3K577_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05994</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79673.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324092.2">
        <property type="nucleotide sequence ID" value="XM_003324044.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K577"/>
    <dbReference type="GeneID" id="10532871"/>
    <dbReference type="KEGG" id="pgr:PGTG_05994"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05994"/>
    <dbReference type="HOGENOM" id="CLU_1826241_0_0_1"/>
    <dbReference type="InParanoid" id="E3K577"/>
    <dbReference type="OrthoDB" id="2511915at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171764" evidence="1">
        <location>
            <begin position="25"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79673.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="141" mass="15625" checksum="9A10810689715F05" modified="2012-04-18" version="2">MRSSRVDCWLLSGFLLISMNVVQSVRVDANHEPGCNAPDDIKSALNIPPRSQCISSSTGVEASGSTPFNKIEFPNDLDFHPNVKNPRTRSRPRFSDPNHFAVAASQIVEPANLTPPFYPKNIRPRDRRYGPALAIAESIRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>E3K7K9</accession>
    <name>E3K7K9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">histidine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Histidyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGTG_06265</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(His) + L-histidine + ATP = L-histidyl-tRNA(His) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.21" evidence="3"/>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324728.1">
        <property type="nucleotide sequence ID" value="XM_003324680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K7K9"/>
    <dbReference type="FunCoup" id="E3K7K9">
        <property type="interactions" value="553"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K7K9"/>
    <dbReference type="GeneID" id="10539316"/>
    <dbReference type="KEGG" id="pgr:PGTG_06265"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06265"/>
    <dbReference type="eggNOG" id="KOG1936">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_025113_4_0_1"/>
    <dbReference type="InParanoid" id="E3K7K9"/>
    <dbReference type="OMA" id="CGGGNFK"/>
    <dbReference type="OrthoDB" id="1906957at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
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        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
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    <feature type="domain" description="WHEP-TRS" evidence="14">
        <location>
            <begin position="9"/>
            <end position="68"/>
        </location>
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    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="13">
        <location>
            <begin position="96"/>
            <end position="469"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="55"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="384"/>
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        </location>
    </feature>
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            <begin position="29"/>
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        </location>
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        <location>
            <begin position="59"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00012815"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51185"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP80309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="592" mass="66031" checksum="C4245D6B5155F5A0" modified="2011-01-11" version="1">MASQSSEQQIEELNMKVKNQGDQVRSLKSAPSEQRDPTQIAQAIETLKNLKSELELLKGTSSTAQEPPKSKTSNQPHAKSTKKEVMTKFQLKVPKGTKDHHPKEMLIREKIFSTITRIFKLHGAVTIDTPVFELKEILSGKYGEDSKLIYDLQDQGGELCSLRYDLTVPFARFLAMNSKEYPSIKRYHIAKVYRRDQPAMTKGRMREFYQCDFDIAGATDPMIADAEVLTIACEVLEALEVGPFTIKLNHRKLLDGIFDLCGVPEDKFRAISSAVDKLDKMAWIDVRKEMTEEKGLDPQIADRIGEYVKLKGSEDLLAQLFEDQTLVANKTAKEGLEEMKLLMSYMNVFGIMPRISFDLSLARGLDYYTGVIYEAVVEGSAPPVKEATASAPMTTEEAVGGPSSKARSTKAKRDETGEAAEIDDESQIGVGSIAAGGRYDNLVGMFSAGKDKIPCVGISFGVERIFSILKSKAAQNRIDNLRGKDVDVFVMSVGDGLLVERMKLCKELWDAGLSAEFMYKSKPKTQKQFEIVDRDQIRFAIILGPEELKSGHVRIKEQVGKESNLHAQDKSGVVVERSQMISLLKERLYNPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3K9V0</accession>
    <name>E3K9V0_PUCGT</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07206</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP80954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003325325.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K9V0"/>
    <dbReference type="GeneID" id="10536112"/>
    <dbReference type="KEGG" id="pgr:PGTG_07206"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07206"/>
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    <dbReference type="InParanoid" id="E3K9V0"/>
    <dbReference type="OrthoDB" id="2497116at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP80954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="304" mass="34381" checksum="9010F9E3A0179B78" modified="2011-01-11" version="1">MISNKSAKQDKLARKLLPNQSIRSDQKKLSSQQCRPPAVCSLPGPVQTEIDWFDCSQNAEKKNEDCLNTLFEIIDSPPSPVTKSRNRGIKTSKGEGNVRQDPSSSDARIPSDNATSSTTGPSNKVLKFIEKNNSNYLKYKANPDFILGLNSTLRYLENLRLILVQLGELSSRSNRPEDEEEEHERGDAKLKNDKTIEMNRIENEEITKILRDLQTIKFIVVFKHHQHLPIINLLSTFVHEINQALHDLDGQQQSMNTRKSIRLVGFPVGLNPPLISKFNVRRLSCFAILDSATGIERLNDFFSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAN0</accession>
    <name>E3KAN0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07058</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP81437.2"/>
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    </dbReference>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07058"/>
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    <dbReference type="InParanoid" id="E3KAN0"/>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81437.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
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</entry>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="418459.E3KB73"/>
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    <dbReference type="KEGG" id="pgr:PGTG_07835"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07835"/>
    <dbReference type="eggNOG" id="ENOG502SG24">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="InParanoid" id="E3KB73"/>
    <dbReference type="OrthoDB" id="159229at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="SOD_Cu/Zn_/chaperone"/>
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        <property type="entry name" value="SOD_Cu_Zn_dom"/>
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    </feature>
    <feature type="chain" id="PRO_5003173188" evidence="1">
        <location>
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        </location>
    </feature>
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        <location>
            <begin position="43"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP81586.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="173" mass="18032" checksum="B587123DE4D74C63" modified="2012-04-18" version="2">MQFWQLLVLVSQLGMIAAVPNQHAVRNSNTAVAFMKGAGGLKGVFVFVPEGNTIKMSIEYSGVPEGQSFAYHIHEKAYTNGNCGSGGGFWNPTRSTMKAPGSCVAHAPGHCASGDLSGKHGKLVGHGAKSTSLRIFDPSLKITGSSQGIIGKTLMIHNAEGTPVACDIIAHKA</sequence>
</entry>
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    <accession>E3KBD4</accession>
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    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    <gene>
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    <organism evidence="2 3">
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07896"/>
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    <dbReference type="InParanoid" id="E3KBD4"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="114" mass="12315" checksum="6B5A77AA3A5E3C52" modified="2011-01-11" version="1">MAAQPTTNNNKTSPPNNNPTLAQANQLNPPAASNPGTTTTAPTTPVPPQFNLTECSKYLFSQKPELYKPPVPSGGWAAVKCKTISSFSSVCLLKTLYQIQQLIYFQLSDGLFLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBI1</accession>
    <name>E3KBI1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
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    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07736"/>
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    <dbReference type="InParanoid" id="E3KBI1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="bZIP_YAP"/>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="3">
        <location>
            <begin position="73"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="192"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="303"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="513"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="92"/>
            <end position="140"/>
        </location>
    </feature>
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        <location>
            <begin position="9"/>
            <end position="32"/>
        </location>
    </feature>
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        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="192"/>
            <end position="213"/>
        </location>
    </feature>
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        <location>
            <begin position="232"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="303"/>
            <end position="330"/>
        </location>
    </feature>
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            <begin position="340"/>
            <end position="372"/>
        </location>
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        <location>
            <begin position="513"/>
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        </location>
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            <begin position="525"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="562"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP81487.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="596" mass="65282" checksum="E43FD7F8FD17A76A" modified="2012-04-18" version="2">MVTYHGISHSHNTAQDLNQSPNLNHPMQHPNSLHTNLNQTHTTHPSSTSSTNHNHHHHHRKSSNDNDEEAIARKKKNADAQAAFRQRRQTYIKSLEDTVSELKGAVTEMEHIVKTTSQELKNQKQHLEHLQSKLSAYETGELKPGAFENNQHCKCCKFAPPEFNPALPTPTNGTHQVRSISTPTTSTMAINSNADCPTPSGPSIVTPTVTGHDNLTRDWPSRQGVPPMFPPDSSSRSTHHNSTQNSPHDVPHPTTSLDYPRNILHHQHHSNSSIHHSSYPRPGCDLALFTPFGAAESPPMANFYSSTEAHHGSSSQQHHPLGTAYSSLGHPQSFDAHHSGTLSSPRMWSHNSQTNPSTYTGTDPNPAANLNGSPKPGLYPIDGSLAEVDSSGFRLHRGMGERSMMRPADYLPSLELRPLPQSYPMVSTLTSPRADSLGVVGPKTRSSALNKRRWNDDRFASHHDNHEFDEAPNSGKTGVKLNGIPAKVARCEQVCTEVSKVVSQMKEEVKAVTTSKTSATPSTAHQSLSPNATSPNSFPSPHNASHNSLKSQRVKFHGPPSQLASSTPPLAGSQLQVQEEPQTEQDNCRYQSMISN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBW4</accession>
    <name>E3KBW4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08225</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EFP81976.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326395.2">
        <property type="nucleotide sequence ID" value="XM_003326347.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KBW4"/>
    <dbReference type="STRING" id="418459.E3KBW4"/>
    <dbReference type="GeneID" id="10534176"/>
    <dbReference type="KEGG" id="pgr:PGTG_08225"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08225"/>
    <dbReference type="HOGENOM" id="CLU_1428634_0_0_1"/>
    <dbReference type="InParanoid" id="E3KBW4"/>
    <dbReference type="OrthoDB" id="8191639at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="12"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81976.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="190" mass="21612" checksum="2DB51620E0C276E8" modified="2012-04-18" version="2">MFPTLKLFHRINCPDNRPPQSTCSRDNCLFNHSQPKPEPEPKPKPRENGTQKQKQSVPPTATSSQPKVEPSEPLKPQPPRIDISKTMTSSHTFPSVRQKLLETLYQEYLKLYQGLKMSDEQASEAARRDSLQEELDCLRASSQKTYRNAVISRVVKIRKRASESLSAFQSLTGTTTEFDQLKQAQQTPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>E3KC56</accession>
    <name>E3KC56_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">CDC20/Fizzy WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_08081</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat CDC20/Fizzy family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EFP81832.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326251.2">
        <property type="nucleotide sequence ID" value="XM_003326203.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KC56"/>
    <dbReference type="FunCoup" id="E3KC56">
        <property type="interactions" value="652"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KC56"/>
    <dbReference type="GeneID" id="10533704"/>
    <dbReference type="KEGG" id="pgr:PGTG_08081"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08081"/>
    <dbReference type="eggNOG" id="KOG0305">
        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="term" value="F:anaphase-promoting complex binding"/>
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        <property type="term" value="F:ubiquitin ligase activator activity"/>
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    </dbReference>
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        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
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    </dbReference>
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        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1905786">
        <property type="term" value="P:positive regulation of anaphase-promoting complex-dependent catabolic process"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cdc20/Fizzy"/>
    </dbReference>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056150">
        <property type="entry name" value="WD40_CDC20-Fz"/>
    </dbReference>
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        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918">
        <property type="entry name" value="CELL DIVISION CYCLE 20 CDC20 FIZZY -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19918:SF8">
        <property type="entry name" value="FI02843P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24807">
        <property type="entry name" value="WD40_CDC20-Fz"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
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        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 7" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="CDC20/Fizzy WD40" evidence="9">
        <location>
            <begin position="271"/>
            <end position="573"/>
        </location>
    </feature>
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        <location>
            <begin position="399"/>
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        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="7">
        <location>
            <begin position="542"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="26"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="222"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="595"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="105"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="121"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="164"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="8">
        <location>
            <begin position="174"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="228"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="251"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006445"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF24807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP81832.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="620" mass="66821" checksum="218C0217900F8E31" modified="2012-04-18" version="2">MAPSPNKRHTISTTVNPLLSNFSMMSLDHLAPPTSSTSTTSGRAKKSSNPSPLKGSKSSSCLRPSSYHHQQQQQQQQQQQQKSQSSTSLGQPLSSSLHKALSRPINRDDLLGRDLVRKDWSSSSSSNTSPSKKTTARKKATSVDRYITRDVDSCQLESSHPRETNSNGTTTGSSSGGGGGSGGGSGGGGGAGAGLESSHALELSTALGIDLNRRILSFAADVPSSSRVTRDRGDEAIGKEQRAAKNGSGNGLSSTASNPRRQVSAIPERVLDAPGLIDDYYLNLTDWSVDNILAIALGESLYLWNAQTGNVNQLCGLEEGSYYASVKFSGDGHYLALGTSEGAVHIYDIDEARLLRKMLGRECRVSSLSWSGTILSAGGQDGSIWNHDVQAARHKSSEMLGHRAEVCGLAWKPELDDLVTLNPSNSGLLASGANDNLVNVWDPRNPSAPRMTKNNHRAAVKAIAWCPWQPNMLATGGGTSDKMVHFWNVNTSSRLQSLETRSQVTSIIFNPYAREFLTTHGLPDMHFSIHTFPGFQLVADVSKAHETRILHSSLSPDGCIVVTASSDENLKFWRVFENKRAKVGLNPVFGKAYPNPLASKDTNQQDHHHPHSFKGGFELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCM4</accession>
    <name>E3KCM4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07573</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82176.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326595.2">
        <property type="nucleotide sequence ID" value="XM_003326547.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCM4"/>
    <dbReference type="STRING" id="418459.E3KCM4"/>
    <dbReference type="GeneID" id="10529548"/>
    <dbReference type="KEGG" id="pgr:PGTG_07573"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07573"/>
    <dbReference type="HOGENOM" id="CLU_548700_0_0_1"/>
    <dbReference type="InParanoid" id="E3KCM4"/>
    <dbReference type="OrthoDB" id="2431475at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40132">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR 38B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40132:SF1">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR 38B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="351"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="424"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="441"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82176.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="485" mass="56361" checksum="EFEEB6550FFCF14D" modified="2012-04-18" version="2">MDITLGRKLDNPQRLSSSADAKKHPYSDSEIAKLLLLESQSYQQSTTYNRLDNSILTGNGLKTNKRFLKSVIRNVNDHNRQLERRPERTLTPAREDEERYPTRPKTDSHSSRSRASRIPDPSDTHSRSSQRSHHPESRAARNDDNDNDDYFCVGGGTATSRRSQTLASSGRDKCKRSGSIVDDGEEKDYRCKEKQKGKERGRERDSDRTHSSSLRDSTTRITHKSGETEDRQAVEEQEEGRKFPSSSKMDKYFSATYDPRLDVNLEDITDPQTGLITGGNYEDWEQILGQMKAKRELKAHEKEHKLRLKLQAAQSKLRKVEKREQRKERKRIKKMEKMDKEKRRRERNEAEEQEVVDQELEDLDRERKRRKRKEKRMMIDQSSSLTRSNHRHQQQQQDDGKDEKDHRKKTKKRKHKAHSDSDSDSSSSSSSSSSSFSDDDDNRHSHDRSALHKKDKLSGVGLIGFNRYSKPGQLREWDLGKPCPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCS5</accession>
    <name>E3KCS5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_07624</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326646.1">
        <property type="nucleotide sequence ID" value="XM_003326598.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCS5"/>
    <dbReference type="STRING" id="418459.E3KCS5"/>
    <dbReference type="GeneID" id="10529600"/>
    <dbReference type="KEGG" id="pgr:PGTG_07624"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07624"/>
    <dbReference type="eggNOG" id="ENOG502S88Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012390_7_0_1"/>
    <dbReference type="InParanoid" id="E3KCS5"/>
    <dbReference type="OMA" id="NAPPRCE"/>
    <dbReference type="OrthoDB" id="2497879at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125">
        <property type="entry name" value="F21J9.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125:SF3">
        <property type="entry name" value="NO-APICAL-MERISTEM-ASSOCIATED CARBOXY-TERMINAL DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="2">
        <location>
            <begin position="176"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP82227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="310" mass="35372" checksum="F89E21CF7429D6F3" modified="2011-01-11" version="1">MLDPMLQQLATPPAQESSTLPVDPLQIQSKRPRASPIEETNRKNDKKQKKEDNAPKNPHWRIEEDKHLCISWLNTSKDTDIGTGQKLSAFWERIHEYFVKLNDSYTKTHKNDKHFKPLPNRAVGALECRWATVLHRVNKYCGAYGQVERRLGSGKTRDEIAVEAKELFKADQGINFTLDHCWAILHNSPKWQETMDEIEAKPKKDKKKIQIPTSEASSVKATTDDESIDAECLGSDRPEGRKAAKKRKHEESEALEGQKELIKISQQKMELMQTVADEAIMGRDLSNLDDLSLAYYAAKKKDIAKRNGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3KD98</accession>
    <name>E3KD98_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Large subunit ribosomal protein L11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_07709</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
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        <property type="protein sequence ID" value="EFP82312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003326683.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KD98"/>
    <dbReference type="FunCoup" id="E3KD98">
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    </dbReference>
    <dbReference type="STRING" id="418459.E3KD98"/>
    <dbReference type="GeneID" id="10529685"/>
    <dbReference type="KEGG" id="pgr:PGTG_07709"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07709"/>
    <dbReference type="eggNOG" id="KOG3257">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_074237_1_0_1"/>
    <dbReference type="InParanoid" id="E3KD98"/>
    <dbReference type="OMA" id="IMSFCKD"/>
    <dbReference type="OrthoDB" id="2496019at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="entry name" value="Ribosomal protein L11/L12, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_uL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_uL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_uL11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_uL11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_uL11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_uL11_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL11M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="Ribosomal L11/L12e N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL11 N-terminal" evidence="7">
        <location>
            <begin position="9"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL11 C-terminal" evidence="6">
        <location>
            <begin position="74"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="87"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP82312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="171" mass="18387" checksum="68B226FE44EE9954" modified="2011-01-11" version="1">MSAPTTNLVRLIVEAGKASPSPPVGPALGSRGIKAMDFCKLFNAQTSHIEPTIPIRVNMTINAKDKSYKFKLNSPPTTYFIKKHLNASSASSSPSSTQSSSSSSSSAKKSNTSSDSKMHSFIGSELSLKFIYHLALIKKSVDHEDLNHIPLESLTRSIAAQVKNMGIKLVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KEA7</accession>
    <name>E3KEA7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_08836</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327059.1">
        <property type="nucleotide sequence ID" value="XM_003327011.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KEA7"/>
    <dbReference type="GeneID" id="10530023"/>
    <dbReference type="KEGG" id="pgr:PGTG_08836"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08836"/>
    <dbReference type="HOGENOM" id="CLU_018638_0_0_1"/>
    <dbReference type="InParanoid" id="E3KEA7"/>
    <dbReference type="OrthoDB" id="2498304at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171935" evidence="2">
        <location>
            <begin position="23"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP82640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="835" mass="94822" checksum="A03A8355A72ADB5B" modified="2011-01-11" version="1">MRTHKPCLAILGFVCFLSSSHSLHISRLQSRALHHAETVLSDSEPLGEAMRNGLQRQPTTPTTGESKLLAPDTQGSGGHSETIKQPVSSEATREGLVPESHPPEPELTKFGKIKNKVMDSYQAFKTKPQFLAGLVKPFENLQKAKEARNQRTTAQRMENFIQIRNQLSKALVEDEKSNGENNLDLDKLAAQLEQKLSKASKPLKSDDNASPFTKAYLDDYLKKLKSHQTIVNQLKNVQKRWSSTEIISPDLTPTELALQSEILSFIREVNEIGFHNRFWEDLHVIQVSINQELKKWPAESFGYLRQHIPNLTKEYLIDKISSRPQKFVAARALQLKGKPLEELPGNSYLTVEQVIERLPSDLGDGEMEMVMYRYAVIKKFEALLQKIRQEATSLLEERLKLSVQPVSPTEANHQLKKLFSSPTKQDSMWLDQTFEKFYGTSPATVFVKLRGIPEDQILMRSVLDNNVESLAFLSEEKMEALRKASSDRQTFQKKLKSISSKLPPGIEKEAIETVRKEATISYPAVERPIVDKLHSQHIISDELKAKLNPPGGLSLEKYLKALGTQDELRKTLMSKFQSDLRKRLEAPQDDPKEILDRTVTLLAQSHIDQLDIRARVKYLKPDSLLSRRPPGFGEALGLYDPEKLTHLADGPQEPAILAKAYVEAHLPLTPLAEYKSDLDELLSEVKIPQIAPDAKFSDDLGFTDRLALFDQRLSAIYLEPFTEDDPRLSGTQYQRLTKESRESLNNLVHTIYSNRRKFDAISQEERLLSGQIEKIEAELAPQLPVVTKVMKATLKPEKPFKRLAKDFFSKKLYTPILRFLKKLMKTAKSKLQKAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KFT4</accession>
    <name>E3KFT4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09172</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EFP83219.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327638.2">
        <property type="nucleotide sequence ID" value="XM_003327590.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KFT4"/>
    <dbReference type="GeneID" id="10544932"/>
    <dbReference type="KEGG" id="pgr:PGTG_09172"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09172"/>
    <dbReference type="HOGENOM" id="CLU_1262089_0_0_1"/>
    <dbReference type="InParanoid" id="E3KFT4"/>
    <dbReference type="OrthoDB" id="2500879at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83219.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="219" mass="23628" checksum="C731A7691E5F6763" modified="2012-04-18" version="2">MALVHPHNPQLVMDVLMKDLSDWAEAMVANAPEVSAQLPPNSVKFLWVDRQKRPTPHPLEAPPGKRNTGPDFTTPPNRPTPASENHPPSEVSEDDDIEVIPISSGATNHATPAASSIPTQPVPPASPELESHDMETYLHVAHIPPNDKLTRAQLLTHGIVHWSFFRASSENELIGLGFPIGIARLLIEGAIEGAGRLARYDHNEGAYTGGMTPSPSPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGG0</accession>
    <name>E3KGG0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Porphobilinogen deaminase</fullName>
            <ecNumber evidence="5">2.5.1.61</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Hydroxymethylbilane synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Pre-uroporphyrinogen synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGTG_08571</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13185"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57845"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="EC" id="2.5.1.61"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>dipyrromethane</name>
            <dbReference type="ChEBI" id="CHEBI:60342"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HMBS family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.61" evidence="5"/>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327804.1">
        <property type="nucleotide sequence ID" value="XM_003327756.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KGG0"/>
    <dbReference type="FunCoup" id="E3KGG0">
        <property type="interactions" value="431"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KGG0"/>
    <dbReference type="GeneID" id="10545101"/>
    <dbReference type="KEGG" id="pgr:PGTG_08571"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08571"/>
    <dbReference type="eggNOG" id="KOG2892">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019704_0_0_1"/>
    <dbReference type="InParanoid" id="E3KGG0"/>
    <dbReference type="OMA" id="LWQANHI"/>
    <dbReference type="OrthoDB" id="564646at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00319"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004418">
        <property type="term" value="F:hydroxymethylbilane synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd13645">
        <property type="entry name" value="PBP2_HuPBGD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.40:FF:000002">
        <property type="entry name" value="Porphobilinogen deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000005">
        <property type="entry name" value="Porphobilinogen deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000086">
        <property type="entry name" value="Probable porphobilinogen deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.40">
        <property type="entry name" value="Porphobilinogen deaminase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00260">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000860">
        <property type="entry name" value="HemC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022419">
        <property type="entry name" value="Porphobilin_deaminase_cofac_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022417">
        <property type="entry name" value="Porphobilin_deaminase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022418">
        <property type="entry name" value="Porphobilinogen_deaminase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036803">
        <property type="entry name" value="Porphobilinogen_deaminase_C_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00212">
        <property type="entry name" value="hemC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557">
        <property type="entry name" value="PORPHOBILINOGEN DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557:SF0">
        <property type="entry name" value="PORPHOBILINOGEN DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01379">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03900">
        <property type="entry name" value="Porphobil_deamC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00151">
        <property type="entry name" value="PORPHBDMNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54782">
        <property type="entry name" value="Porphobilinogen deaminase (hydroxymethylbilane synthase), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00533">
        <property type="entry name" value="PORPHOBILINOGEN_DEAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="9" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Porphobilinogen deaminase N-terminal" evidence="14">
        <location>
            <begin position="67"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="domain" description="Porphobilinogen deaminase C-terminal" evidence="15">
        <location>
            <begin position="311"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="20"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00033064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF03900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EFP83385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="461" mass="50210" checksum="2801A9F10A9E43DA" modified="2011-01-11" version="1">MDQQNLQPTLESYPDLPHQPQRTHATSPSSGPKGSTRLIDLELRELSSSGNKSGLEINSTLSLTDNLIIGTRNSKLALVQAESVEREINKRFSSDQIKTEIKSMSTSGDENLLKPLYLLGGKSVWTKELEFNLLNGSIDLIVHSLKDVPTSLPAGCELASVMLREDPRDALIIRNGLPFTNLSQLPAGSIIGTSSVRRVAQLRRRFPNLAFQDIRGNLQTRFKKLDASDSPYTGIILAVAGLKRIGLAERITSYLEPPDLYHAVGQGAIGIEIRTPPEIKLNETVEETILRERATTVRRLVKSLEDWRTALSCEAERALLRRLEGGCSVPVGVTSSLVDLPMPCPWAPKARLTLTGVVTSVDGLEEVRKEAWLDVCGPKDAAHLGTALADELFRDGADKILDQLNSVKKLDLEEGAASESTSGSSNPSRIATLESQSHFVIAAPANSSRTDIGSNQDHVVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGV0</accession>
    <name>E3KGV0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">3'(2'),5'-bisphosphate nucleotidase</fullName>
            <ecNumber evidence="3">3.1.3.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_08711</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>3'-phosphoadenylyl sulfate + H2O = adenosine 5'-phosphosulfate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77639"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58243"/>
            <dbReference type="ChEBI" id="CHEBI:58339"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="9">
            <dbReference type="Rhea" id="RHEA:77640"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>adenosine 2',5'-bisphosphate + H2O = AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:77643"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:194156"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:77644"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>adenosine 3',5'-bisphosphate + H2O = AMP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58343"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="3.1.3.7"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:10041"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the inositol monophosphatase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.7" evidence="3"/>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327944.1">
        <property type="nucleotide sequence ID" value="XM_003327896.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KGV0"/>
    <dbReference type="FunCoup" id="E3KGV0">
        <property type="interactions" value="63"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KGV0"/>
    <dbReference type="GeneID" id="10532206"/>
    <dbReference type="KEGG" id="pgr:PGTG_08711"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08711"/>
    <dbReference type="eggNOG" id="KOG1528">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033446_1_1_1"/>
    <dbReference type="InParanoid" id="E3KGV0"/>
    <dbReference type="OMA" id="MSYQQER"/>
    <dbReference type="OrthoDB" id="411145at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008441">
        <property type="term" value="F:3'(2'),5'-bisphosphate nucleotidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000103">
        <property type="term" value="P:sulfate assimilation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01517">
        <property type="entry name" value="PAP_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.80:FF:000003">
        <property type="entry name" value="PAP-specific phosphatase HAL2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006239">
        <property type="entry name" value="DPNP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051090">
        <property type="entry name" value="Inositol_monoP_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01330">
        <property type="entry name" value="bisphos_HAL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43200:SF6">
        <property type="entry name" value="3'(2'),5'-BISPHOSPHATE NUCLEOTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43200">
        <property type="entry name" value="PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044479"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP83525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="427" mass="46896" checksum="9D9B9E6878F16A6B" modified="2011-01-11" version="1">MPAAFQIQRIGISSSRLFGCSRTLHSKPIPQLLDQRNSTKAMVIPIQSGYQLEREVAVAAVLQASLVTRRIFDKLIRPGLQTGDNNQASITKVDRSPVTVGDYTVQALLNFILSKYFPDDEIVGEEDSSELLKTTDKKHLQQIIDFTNEGLKEDRLSIPTDEEKWSKFRSQPALTEDELVKLIDLGKSAGGKPGENRRFWTLDPIDGTKGFLRGGQYAICLALIVDGEAVLGVIGTPNLPLKGIPSPTDTEPTGVLFLAEKGSGAFQRALGVDEYTEIKMKPHERGSLGREGTFCESFDAGHSNQLVTGDIARKLNMLNAQSPIRIDSQAKYCVLARGDSDVYLRFPTQADYQEKIWDHAAGSIIISEAGGKVVDLDGKPLDFSGGRTLSNNHPGFLACHNQVLVDVLKAAKAAHENQLKQQKLHKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJ34</accession>
    <name>E3KJ34_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10029</name>
        <name evidence="3" type="ORF">PGTG_12118</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178290">
        <property type="protein sequence ID" value="EFP84309.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="DS178298">
        <property type="protein sequence ID" value="EFP86162.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328728.2">
        <property type="nucleotide sequence ID" value="XM_003328680.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330581.2">
        <property type="nucleotide sequence ID" value="XM_003330533.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJ34"/>
    <dbReference type="GeneID" id="10540171"/>
    <dbReference type="GeneID" id="10545660"/>
    <dbReference type="KEGG" id="pgr:PGTG_10029"/>
    <dbReference type="KEGG" id="pgr:PGTG_12118"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10029"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12118"/>
    <dbReference type="HOGENOM" id="CLU_2373827_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJ34"/>
    <dbReference type="OrthoDB" id="2510100at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84309.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP86162.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="95" mass="10259" checksum="809FD47B7E7A0EBB" modified="2012-04-18" version="2">MSVGVFHGHPTSWEQQEQEQAITATGTSKGSAPTGQNWSDNSVQPLPGCPISSWRQTGQRHLYGHCPVRSARSDHQSMGAAVTFGKKNLGLQLVM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJQ3</accession>
    <name>E3KJQ3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chromo domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84528.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328947.2">
        <property type="nucleotide sequence ID" value="XM_003328899.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJQ3"/>
    <dbReference type="STRING" id="418459.E3KJQ3"/>
    <dbReference type="GeneID" id="10546121"/>
    <dbReference type="KEGG" id="pgr:PGTG_10687"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10687"/>
    <dbReference type="HOGENOM" id="CLU_1262062_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJQ3"/>
    <dbReference type="OrthoDB" id="3647690at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        <location>
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            <end position="96"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84528.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="219" mass="24638" checksum="19A39F9DD61CF060" modified="2012-04-18" version="2">MKKTNPRDKEWAAIQILEERGGKYLIEWAGIDPSTQKSWAPTWEPKKLANKALAREWQHKKRRQSQATLPNTKDKSSIPVDSPNTTLPGGDTQTEGSNDLAEVNIHIKASQEDELEDELIPLAEFVPAPITTPEQYTTIVDDLPLHVVQADPNLRGQQTAMFIVGDNWVEISGLGFIMDPRLTHPNCSQDRFVLSGEASPRDWPDHLKQFFSSQGITFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KL36</accession>
    <name>E3KL36_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_11180</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP85011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329430.1">
        <property type="nucleotide sequence ID" value="XM_003329382.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KL36"/>
    <dbReference type="GeneID" id="10526988"/>
    <dbReference type="KEGG" id="pgr:PGTG_11180"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11180"/>
    <dbReference type="HOGENOM" id="CLU_096176_0_0_1"/>
    <dbReference type="InParanoid" id="E3KL36"/>
    <dbReference type="OMA" id="VAYVACF"/>
    <dbReference type="OrthoDB" id="2509560at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173964" evidence="2">
        <location>
            <begin position="18"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP85011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="207" mass="22694" checksum="74E5216C2E2BBF3E" modified="2011-01-11" version="1">MRSFIAVAYILAAFAAATPVDLQARQISSSSVQAASGHDATHQSSTNSSPFGFSHQESDSEHSFNQYSSVTNSMAPVFGTIGQIQNLMSQGSFSESSAMSYMTRLVQQLQPAFDGIRGCNCLQDPTVVSSYNRMFSQLYQIFQFCQSQYGQNFNQLVAPFGGLFNSFSQFQSEAHQYQSFSQFSNSFNPLVSMLGSANPSFNTLGQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLN8</accession>
    <name>E3KLN8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_11406</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C15 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178294">
        <property type="protein sequence ID" value="EFP85237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329656.1">
        <property type="nucleotide sequence ID" value="XM_003329608.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLN8"/>
    <dbReference type="STRING" id="418459.E3KLN8"/>
    <dbReference type="GeneID" id="10527226"/>
    <dbReference type="KEGG" id="pgr:PGTG_11406"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11406"/>
    <dbReference type="eggNOG" id="KOG4755">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043960_0_0_1"/>
    <dbReference type="InParanoid" id="E3KLN8"/>
    <dbReference type="OMA" id="TWRSFAP"/>
    <dbReference type="OrthoDB" id="407146at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008234">
        <property type="term" value="F:cysteine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.20:FF:000015">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.20">
        <property type="entry name" value="Peptidase C15, pyroglutamyl peptidase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016125">
        <property type="entry name" value="Peptidase_C15-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036440">
        <property type="entry name" value="Peptidase_C15-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23402">
        <property type="entry name" value="PROTEASE FAMILY C15 PYROGLUTAMYL-PEPTIDASE I-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23402:SF1">
        <property type="entry name" value="PYROGLUTAMYL-PEPTIDASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53182">
        <property type="entry name" value="Pyrrolidone carboxyl peptidase (pyroglutamate aminopeptidase)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="130"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP85237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="276" mass="31686" checksum="904585CF192D1D2B" modified="2011-01-11" version="1">MPGGLYAACRDLDVRDMYIENINHHVQVYRNRHEKANDQHPKYAQYTDSTTHQDTFNRIWWFDSEDGNSAVELVVHPEPVRVSYRTVDELLSRLYQTHPDLDLVIHTGIAQLPRAEFLLEKIAHREHYKSPDVDGLLPDPSLSDSETRPGSLSSSVMLDQLVERVQDQISQPINLKVSENAGRFLCEYIYYSSLLRFENLTNSNPEPPSTSVFKKEEADRARPRPVLFLHVPPFLDQADITLGSHVLLDIIHQVQALHFSSSSDNLYKKFLGSSYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>E3KM05</accession>
    <name>E3KM05_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glutamate--tRNA ligase, mitochondrial</fullName>
            <ecNumber evidence="3">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glutamyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_11523</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="3"/>
    <dbReference type="EMBL" id="DS178294">
        <property type="protein sequence ID" value="EFP85354.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329773.2">
        <property type="nucleotide sequence ID" value="XM_003329725.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KM05"/>
    <dbReference type="FunCoup" id="E3KM05">
        <property type="interactions" value="386"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KM05"/>
    <dbReference type="GeneID" id="10543897"/>
    <dbReference type="KEGG" id="pgr:PGTG_11523"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11523"/>
    <dbReference type="eggNOG" id="KOG1149">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015768_6_3_1"/>
    <dbReference type="InParanoid" id="E3KM05"/>
    <dbReference type="OrthoDB" id="428822at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00808">
        <property type="entry name" value="GluRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000045">
        <property type="entry name" value="Glutamate--tRNA ligase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00022">
        <property type="entry name" value="Glu_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045462">
        <property type="entry name" value="aa-tRNA-synth_I_cd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004527">
        <property type="entry name" value="Glu-tRNA-ligase_bac/mito"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049940">
        <property type="entry name" value="GluQ/Sye"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033910">
        <property type="entry name" value="GluRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00464">
        <property type="entry name" value="gltX_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43311">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43311:SF2">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19269">
        <property type="entry name" value="Anticodon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="An anticodon-binding domain of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic" evidence="12">
        <location>
            <begin position="32"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class I anticodon-binding" evidence="13">
        <location>
            <begin position="376"/>
            <end position="530"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00072917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF19269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EFP85354.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="531" mass="60480" checksum="7FA3AA60D3D79C3B" modified="2012-04-18" version="2">MMRCRAILPLHRLSKQFFGTSTRRTAHSEQPVRLRFAPSPTGYLHLGGLRTALFNHLLARKLRGKWILRIEDTDQSRLVDGAVESIIETLEWAKLDYDEGPNRPGECGPYIQSQRQTIYQAHRDQLLQSGQAYRCFCSPDRLDQMRQEFRSSGSNLTYDRKCLQLSEAEVTEKMSRGLSHVIRLKNSDNPVSVDDLVYGQLTFKNNTQDDVILIKADGTPTYHFANVVDDHHMGISHVLRGEEWLSSTPKHILLYNAFGFPLPRFAHLPLLVNADGSKLSKRSGDVRVEDYIAKGYEPEALLNFVALMGMNHSRHTTDEGKNENTTTDVMTMEQMVSAFSIDVIGKHRSTMSQPKLVHLNQQHIARNLLSTEPDQVQPWITRARSILLCGPARPEVSDAYVHRVLLAMKDRLHVFNDIKYLAEYFFKLPDLSSSEAREMRKNIPDELYHRILDSSHQMLSEFPEEETGYEREKVAAGLKQVREADPELSSKQVMVALRHAITGRKIGPGMAETIGVLGKKRTLERIEAALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>E3KM69</accession>
    <name>E3KM69_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RanBD1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_11386</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178294">
        <property type="protein sequence ID" value="EFP85217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329636.1">
        <property type="nucleotide sequence ID" value="XM_003329588.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KM69"/>
    <dbReference type="FunCoup" id="E3KM69">
        <property type="interactions" value="643"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KM69"/>
    <dbReference type="GeneID" id="10527206"/>
    <dbReference type="KEGG" id="pgr:PGTG_11386"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11386"/>
    <dbReference type="eggNOG" id="KOG0864">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067861_1_1_1"/>
    <dbReference type="InParanoid" id="E3KM69"/>
    <dbReference type="OMA" id="NFKDSFM"/>
    <dbReference type="OrthoDB" id="2357150at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006913">
        <property type="term" value="P:nucleocytoplasmic transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13179">
        <property type="entry name" value="RanBD_RanBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.29.30:FF:000312">
        <property type="entry name" value="Ran binding protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045255">
        <property type="entry name" value="RanBP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045256">
        <property type="entry name" value="RanBP1_RanBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138:SF87">
        <property type="entry name" value="E3 SUMO-PROTEIN LIGASE RANBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23138">
        <property type="entry name" value="RAN BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00160">
        <property type="entry name" value="RanBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50196">
        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RanBD1" evidence="2">
        <location>
            <begin position="21"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="216"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP85217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="252" mass="27857" checksum="340A0FDEF5ECA17B" modified="2011-01-11" version="1">MSQPQDEQPVTSDATLEHDPDYQPLVHLTQIETKTHEENEDVFFKLRAKLFRFDKTASEWKERGTGELKLLQDNTTKRIRLVMRRDKTLKVCANHFITAEMVLAPNVGSDRSWVYNTTADVGDEGVTAETLAIRFGNSENANAFKAKFEEAQKINGGLTKEPSTTTSTDPAQPEESSTTKKEAKEEEAAAPGSKTEEKKSSEEVAESKESAPVVQSDATAQAVVETTTSETQKDAEPSDAAKVESKDDDKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3KMA4</accession>
    <name>E3KMA4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_11785</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178295">
        <property type="protein sequence ID" value="EFP85429.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11785"/>
    <dbReference type="HOGENOM" id="CLU_409461_0_0_1"/>
    <dbReference type="InParanoid" id="E3KMA4"/>
    <dbReference type="OrthoDB" id="2505741at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="295"/>
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        </location>
    </feature>
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        <location>
            <begin position="421"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="593"/>
            <end position="622"/>
        </location>
    </feature>
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        <location>
            <begin position="494"/>
            <end position="585"/>
        </location>
    </feature>
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        <location>
            <begin position="76"/>
            <end position="92"/>
        </location>
    </feature>
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        <location>
            <begin position="106"/>
            <end position="115"/>
        </location>
    </feature>
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        <location>
            <begin position="130"/>
            <end position="149"/>
        </location>
    </feature>
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        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
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        <location>
            <begin position="213"/>
            <end position="222"/>
        </location>
    </feature>
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        <location>
            <begin position="301"/>
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        </location>
    </feature>
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            <begin position="458"/>
            <end position="478"/>
        </location>
    </feature>
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        <location>
            <begin position="593"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP85429.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="673" mass="73206" checksum="2D16407638B41849" modified="2012-04-18" version="2">MSRSTTTLNGSRSNAQTIGRRPAQTQAATPNDSTRSILLKNNSSLSQLKPSRSRLTTQSKNLVEPRTPATLGRRVNNLNLLSGRNSPSQTPLVTIKRDGKSRLPRAAPTPTAQTPGISNPGTKKLRVKKSSTLQAQPATPARSSSSSLALTPEPPTALQTEVPKPGSSTHLTTVDPDATPKKPPSRSSFKQNAVLTSSEDQPSSKLPLPHTITRPTPQTSIVFPSIVEEQTPKRPMTRARSKQILDRSTSSLGVSPPEQTGISQIKRKPSRVMQIAQAFQQESQLLTDGHSKNMLGVTPQRRPSNGSNTSAVSPTGSLVGNSSPNISALAASLSKQHGQRDRLSKARSSVTDMLRYSTMSTLGGLHWEDGAGEGLLTDPDETEAMVADISMSNTPLKPAVQSAAIALASARANFLTIVQENEQDPPKKISHKQSESSLNTTSSRIKKRPSFGFIPASTPLSRSRISGTSFTSNSPIINQQQQQQQQMVIHETQLATLSASLERSQQREIELEKEIQKLEQRSEHENSLKKLSDQEREKALVEEIGRLEEEVSGLQELNIELENSLDSVQFDNLELRNKLDRLQIDHHRLLALSPSDSSSSSNLPPFSSITLNSQPHSTPNLNSNLPLNHSPRCSKFEAFELEIVAEIAAIKDQLKLIQFIKISLALGSDLVSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNB9</accession>
    <name>E3KNB9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EFP85755.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003330126.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNB9"/>
    <dbReference type="GeneID" id="10544321"/>
    <dbReference type="KEGG" id="pgr:PGTG_11084"/>
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    <dbReference type="InParanoid" id="E3KNB9"/>
    <dbReference type="OrthoDB" id="10292311at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="183"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85755.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="499" mass="56109" checksum="07C35611C7117680" modified="2012-04-18" version="2">MFHNKLQVTRTSLGISKLEGKGVASVTNLYTGKMLPFKYSTHLLLAHLGLSIAGKALERPAYDPFHQILDWGDVPPNLNSGLLDSTQAPAEAQRRLKVPTLAIPTSSGTELAQTGPFRNGLSMDNVPNDIDLGASLPPLLPNGLKEFNSGTSNQQLLQNFNAYSSVAGEVLEKSPRYIEGAPSCTTSRDGSENHISYTPQSERNEAVRCHQTWRRYKKTADNTVDPISTPSLQVQSSEPFPAWFANSPNLSFDSLSKDSLPPDHTSIYDSSINEINEPSSFSHLESPSYKRLRFTDHFMDPKTPDYNSKEWDILLDYLPIESEPTAQMEFRENHSNIGTSSHVANQVNQRHTNFPLETGGNYGRMVDFQTEPQSIMVEQADINLYLRAISHPNVDAKAALNPHEKKMKMFKFDMDVFENCDAIGLDALKIKRIRTLIEKSAKKELLVSDISYGEVHQNYFHKLSRFEPNDPRTPTDYEGLTVDEYSRMGHQRKRLESFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPL7</accession>
    <name>E3KPL7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RlpA-like protein double-psi beta-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_12208</name>
    </gene>
    <organism evidence="4 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <reference evidence="4" key="3">
        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178299">
        <property type="protein sequence ID" value="EFP86252.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="DS178299">
        <property type="protein sequence ID" value="EHS63827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330671.2">
        <property type="nucleotide sequence ID" value="XM_003330623.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889426.1">
        <property type="nucleotide sequence ID" value="XM_003889377.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KPL7"/>
    <dbReference type="STRING" id="418459.E3KPL7"/>
    <dbReference type="GeneID" id="10540263"/>
    <dbReference type="KEGG" id="pgr:PGTG_12208"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12208"/>
    <dbReference type="eggNOG" id="ENOG502S6X4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_963590_0_0_1"/>
    <dbReference type="InParanoid" id="E3KPL7"/>
    <dbReference type="OrthoDB" id="623670at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22191">
        <property type="entry name" value="DPBB_RlpA_EXP_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051477">
        <property type="entry name" value="Expansin_CellWall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836:SF27">
        <property type="entry name" value="RLPA-LIKE PROTEIN DOUBLE-PSI BETA-BARREL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5010830839" description="RlpA-like protein double-psi beta-barrel domain-containing protein" evidence="2">
        <location>
            <begin position="26"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="RlpA-like protein double-psi beta-barrel" evidence="3">
        <location>
            <begin position="190"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP86252.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS63827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="296" mass="32056" checksum="4D31B3BE04607959" modified="2012-04-18" version="2">MHSTRMSTLGCLVFTFSISAHYGHAADFQNFIPASTSPITSPTTEFPAQLDGRSMENLASHSTIFRKERRSGASSVTTNDTASLVVRPRWKKCSRGIKSRYCRSYHRKLKNQKFSLKTDASLKLGSVKAVSIPENNIPPTVSIPKIEVPSTPHVPKIEVPSTPYVPKIEVPSTPSVHKIEDSRKDSGPKSGDATYYGTGMGACGIVSNDNSMIAAASHLLFDSFPGATANPNLNPICGRKVKATYQGKSVVVELVDRCTGCALHDLDFSPAAFAQIGPMDRGRLHGMTCAWMLTLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQ45</accession>
    <name>E3KQ45_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cofilin</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Actin-depolymerizing factor 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_12376</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the actin-binding proteins ADF family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178300">
        <property type="protein sequence ID" value="EFP86420.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330839.2">
        <property type="nucleotide sequence ID" value="XM_003330791.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQ45"/>
    <dbReference type="FunCoup" id="E3KQ45">
        <property type="interactions" value="138"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KQ45"/>
    <dbReference type="GeneID" id="10540435"/>
    <dbReference type="KEGG" id="pgr:PGTG_12376"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12376"/>
    <dbReference type="eggNOG" id="KOG1735">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_094004_3_2_1"/>
    <dbReference type="InParanoid" id="E3KQ45"/>
    <dbReference type="OrthoDB" id="10249245at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015629">
        <property type="term" value="C:actin cytoskeleton"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016363">
        <property type="term" value="C:nuclear matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030042">
        <property type="term" value="P:actin filament depolymerization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051014">
        <property type="term" value="P:actin filament severing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11286">
        <property type="entry name" value="ADF_cofilin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.20.10">
        <property type="entry name" value="Severin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002108">
        <property type="entry name" value="ADF-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029006">
        <property type="entry name" value="ADF-H/Gelsolin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017904">
        <property type="entry name" value="ADF/Cofilin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11913">
        <property type="entry name" value="COFILIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00241">
        <property type="entry name" value="Cofilin_ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00102">
        <property type="entry name" value="ADF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55753">
        <property type="entry name" value="Actin depolymerizing proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51263">
        <property type="entry name" value="ADF_H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ADF-H" evidence="6">
        <location>
            <begin position="2"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP86420.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="138" mass="15665" checksum="7CE850EDF82E63A6" modified="2012-04-18" version="2">MSSGVTVHPQCMHDFIDLKLKKKSKYIIYAISDDSKEIVVEKVSEAQSYDDFLEDLPSGSCRYAVYDFEYCAEETEGKRNKLCFFTWSPDDAKIKNKMVYAASKRALREALVGIALEIQGTDASEVAYQTVLEKALKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KRC3</accession>
    <name>E3KRC3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13230</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178302">
        <property type="protein sequence ID" value="EFP86848.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331267.2">
        <property type="nucleotide sequence ID" value="XM_003331219.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KRC3"/>
    <dbReference type="GeneID" id="10541597"/>
    <dbReference type="KEGG" id="pgr:PGTG_13230"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13230"/>
    <dbReference type="HOGENOM" id="CLU_012385_0_0_1"/>
    <dbReference type="InParanoid" id="E3KRC3"/>
    <dbReference type="OrthoDB" id="2507457at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="388"/>
            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="514"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="540"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="559"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="599"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86848.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="647" mass="71830" checksum="4134029417C088B1" modified="2012-04-18" version="2">MSPHEFPQGHPHHIPLGQSPTCAANQNPSTGHTASQTPATAQFRPVLYTRSPLGNGTANQSECDPQLAPSKSNQPLSSGYVASSLNGSLPPTMSFEPGATSSKPAKKPRKKRAPRKKAPTQPLEISPPRNTVPSITHPKETIIDPTDPTQRPKRHRVDAETLDKIRGEPLDQLRARAVKYAEYERLTAEDKLALDEAYREYQTQVHLIAIERKLHPKPVLQYLGNKVRIRGPSNFNNFCVYDQVAGPIYHDKAMSISDRMSECARLWHLLDETTKNQWRDQDFLDSISPPDNIAPPAQDGAEAFARWKKHDKFKLTLWIRKIKRDLKNLSVSHQVEGFFVLASRDPDSPIIKTGGSLMAEEFLDLMAADTNPCSLFYRFVNGQQAIKDISGSYPKPTNKRKRRSGKDDDENCPHDLGSKKANIEAVREKLKGALRQATHGVWQGGWPGTKTESTLASLGVTLQVQQNDKCVLPIDFCARPSDMRNAQAQRILTAFAEGWVRLLGPPPPEVGVGMSNSIGHEIDDDDSDASPTRVTITAAKRPKVTKKQQAKKRASAAGDETRPTPEPEPIKQKRVKPKRAVAEKKSLSTQAVGIIRQPRSVTPPDSQSPTPSIDRLANSEDKSDVSEFVPRKRGRILIESDEESDDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KRY0</accession>
    <name>E3KRY0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13274</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EFP87055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331474.1">
        <property type="nucleotide sequence ID" value="XM_003331426.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KRY0"/>
    <dbReference type="GeneID" id="10542136"/>
    <dbReference type="KEGG" id="pgr:PGTG_13274"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13274"/>
    <dbReference type="HOGENOM" id="CLU_1928636_0_0_1"/>
    <dbReference type="InParanoid" id="E3KRY0"/>
    <dbReference type="OrthoDB" id="10329787at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173654" evidence="1">
        <location>
            <begin position="25"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="131" mass="14694" checksum="A27DE268FB332466" modified="2011-01-11" version="1">MLFSKTSLACIVLYALGASQLVLSTSIEGARRFICVNTHQELVDKYPQQNQHAALEMTSDFARVISTLTRPDMIDVADEQVRGLKSRIPQLTHQARQISPEFSADFTQVLANLARQYTEKSILLKSNARKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3KS84</accession>
    <name>E3KS84_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_13378</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EFP87159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331578.1">
        <property type="nucleotide sequence ID" value="XM_003331530.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KS84"/>
    <dbReference type="STRING" id="418459.E3KS84"/>
    <dbReference type="GeneID" id="10537389"/>
    <dbReference type="KEGG" id="pgr:PGTG_13378"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13378"/>
    <dbReference type="eggNOG" id="KOG1994">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046724_1_0_1"/>
    <dbReference type="InParanoid" id="E3KS84"/>
    <dbReference type="OMA" id="DYMNMVI"/>
    <dbReference type="OrthoDB" id="786951at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025239">
        <property type="entry name" value="DUF4187"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039249">
        <property type="entry name" value="GPATCH11"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21032">
        <property type="entry name" value="G PATCH DOMAIN-CONTAINING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21032:SF0">
        <property type="entry name" value="G PATCH DOMAIN-CONTAINING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13821">
        <property type="entry name" value="DUF4187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01173">
        <property type="entry name" value="DUF4187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G-patch" evidence="2">
        <location>
            <begin position="102"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP87159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="313" mass="35602" checksum="BC67ED83A2B46448" modified="2011-01-11" version="1">MGPCRSNSSNSTRAKPVNLHDGQEADEEEEDFMSDKFLKQITETSTENKTYSEKRKRRLIDASTNHQKRSRVQREIEAREEGLSRNLIQANVVQEEKAPPESHSKAFQMMLKMGFQPGNSLGASASNGSNEPINIVPRSGRAGIGIAPKPVSAAKLPRLLFSVKGKMDLTEEETAQREAFLATSRTRFDGRKVDAILGRACRTCEELDRRRGLESNIFWIIKPSPQDESREDAHLTRLDRLVSLDCQNDPDNILEDLEDEEKQNWLALDPSTRLSCTLGYLREEYFYCIWCGCQYESLEELNKECPGEEEDDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KSF6</accession>
    <name>E3KSF6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CBM1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12826</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178305">
        <property type="protein sequence ID" value="EFP87242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331661.1">
        <property type="nucleotide sequence ID" value="XM_003331613.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KSF6"/>
    <dbReference type="GeneID" id="10537641"/>
    <dbReference type="KEGG" id="pgr:PGTG_12826"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12826"/>
    <dbReference type="HOGENOM" id="CLU_2360775_0_0_1"/>
    <dbReference type="InParanoid" id="E3KSF6"/>
    <dbReference type="OrthoDB" id="2507909at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173848" description="CBM1 domain-containing protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="96" mass="10372" checksum="8FCF1B5A3322E95E" modified="2011-01-11" version="1">MNVIKFALLAFVFGVSNVMSKNLKPCGGNTPAYACADDPSRSPDPLIYIPPTDLDCTLTPSEVPWCCPQGAISPNHPSKLGYAIDHGCQKRQPRVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3KSV7</accession>
    <name>E3KSV7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13715</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178306">
        <property type="protein sequence ID" value="EFP87487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331906.1">
        <property type="nucleotide sequence ID" value="XM_003331858.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KSV7"/>
    <dbReference type="GeneID" id="10538385"/>
    <dbReference type="KEGG" id="pgr:PGTG_13715"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13715"/>
    <dbReference type="HOGENOM" id="CLU_1866143_0_0_1"/>
    <dbReference type="InParanoid" id="E3KSV7"/>
    <dbReference type="OrthoDB" id="2511760at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="137" mass="15327" checksum="585BCEB72D728CD3" modified="2011-01-11" version="1">MSHSLYSASNLTQTQRSVHHQSALLQPSTGVWQAGHPREQLFNHDRYSPSSPPSAFLPYLIPLEPIVHHSNNKTQTEGFFLRLNHLLTLGLVDSALSTPRLQSTLHAVYTKHTFICRSNLPLRVLNTALIQASRATP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3KTX6</accession>
    <name>E3KTX6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13457</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178308">
        <property type="protein sequence ID" value="EFP87671.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332090.2">
        <property type="nucleotide sequence ID" value="XM_003332042.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KTX6"/>
    <dbReference type="GeneID" id="10538903"/>
    <dbReference type="KEGG" id="pgr:PGTG_13457"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13457"/>
    <dbReference type="HOGENOM" id="CLU_3125689_0_0_1"/>
    <dbReference type="InParanoid" id="E3KTX6"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87671.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="50" mass="5204" checksum="E26B397F6097ECEA" modified="2012-04-18" version="2">MSGRTSAGGISPRRVAMRGAPGTPGAPCPVEVLPERRVHRAPSLWNNGVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KUH4</accession>
    <name>E3KUH4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13728</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178310">
        <property type="protein sequence ID" value="EFP87924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332343.1">
        <property type="nucleotide sequence ID" value="XM_003332295.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KUH4"/>
    <dbReference type="GeneID" id="10534903"/>
    <dbReference type="KEGG" id="pgr:PGTG_13728"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13728"/>
    <dbReference type="HOGENOM" id="CLU_138087_0_0_1"/>
    <dbReference type="InParanoid" id="E3KUH4"/>
    <dbReference type="OrthoDB" id="10571702at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="58"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="104" mass="11981" checksum="B6AD6284CD1F2A28" modified="2011-01-11" version="1">MVPDISKFKTDIWQICYSQSQKFQELGFTNNPYTKGGERENWDPLSGEERRPKTNHMYSQPSHYSSNSHNYHSSQGENPHQTNHQEMRGDTSSLRNSGNPSSGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KUN4</accession>
    <name>E3KUN4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13788</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178310">
        <property type="protein sequence ID" value="EFP87984.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003332355.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KUN4"/>
    <dbReference type="GeneID" id="10535043"/>
    <dbReference type="KEGG" id="pgr:PGTG_13788"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13788"/>
    <dbReference type="HOGENOM" id="CLU_2293067_0_0_1"/>
    <dbReference type="InParanoid" id="E3KUN4"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87984.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="101" mass="11055" checksum="8C30927A0A8E78B1" modified="2012-04-18" version="2">MTQTAKLSERRARLNWSPSILQLRHPHNQQTQFTMQFNVLPALMLIIAASAILEATAVPIANIMRFKGFTSKTLAKNTTTANATAAGQPVGTKQMAKGFGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>E3KV85</accession>
    <name>E3KV85_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="12">CDF family cation efflux system protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_14264</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family. SLC30A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178312">
        <property type="protein sequence ID" value="EFP88180.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332599.2">
        <property type="nucleotide sequence ID" value="XM_003332551.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KV85"/>
    <dbReference type="FunCoup" id="E3KV85">
        <property type="interactions" value="115"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KV85"/>
    <dbReference type="GeneID" id="10535488"/>
    <dbReference type="KEGG" id="pgr:PGTG_14264"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14264"/>
    <dbReference type="eggNOG" id="KOG1483">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013430_4_3_1"/>
    <dbReference type="InParanoid" id="E3KV85"/>
    <dbReference type="OrthoDB" id="2503918at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006882">
        <property type="term" value="P:intracellular zinc ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071577">
        <property type="term" value="P:zinc ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1510.10:FF:000061">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1510.10:FF:000026">
        <property type="entry name" value="Zinc/cadmium resistance protein-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45820">
        <property type="entry name" value="FI23527P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45820:SF4">
        <property type="entry name" value="ZINC TRANSPORTER 63C, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="Cation efflux protein cytoplasmic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="79"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="310"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="354"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="10">
        <location>
            <begin position="12"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="10">
        <location>
            <begin position="297"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein cytoplasmic" evidence="11">
        <location>
            <begin position="384"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="142"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="258"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="468"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="142"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="163"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="192"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="278"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="292"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP88180.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="531" mass="57428" checksum="F04D66149B76BF1E" modified="2012-04-18" version="2">MGLDSSRSLRIKVLLLIDTAFFFLELIVGYSVGSLALVADSFHMLNDVCSLLVALYAIKLAGNSKRSHEYSYGWQRAEVLGALINGVFLLALCFSIFLEAIQRVFDPINISSPPLVVLVGSLGLASNIVGLLLFHEHGHAHGGGHSHHAHSRSHSHPEHLPVSSHSENNHQHLPTNLDQRVSQPGPTDEHTPLLTPASAPSSSPMVRQPSSSSISSYDSSSTGTISPKPDKRQSLICVHPAQTRNNILRAAELQRDQLTHHHSSARTQPLPKADITDNLPTQPTSDPEASHPHHHSSHSHSHSHMNMRAIFLHALGDALGNVGVIVTGLLIWLVPTIDRHGNAGKNGWIVYADPTISLVITGIIFTSALPLVRSASLILLQGTPSHVNLGRVQKSLEAIKGVLQVHELHIWSLSELKLVASVHVLIKNQDDFVTISRHIRKCLHHYGIHSSTIQPEILLEEQVNSLRAMSTTSSASPPARSRPASTPTTDVANLIDVDGCLQQCDETCDQEACCPPGSVQPRIPAGDSTAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVE0</accession>
    <name>E3KVE0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14319</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178312">
        <property type="protein sequence ID" value="EFP88235.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332654.2">
        <property type="nucleotide sequence ID" value="XM_003332606.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KVE0"/>
    <dbReference type="GeneID" id="10535545"/>
    <dbReference type="KEGG" id="pgr:PGTG_14319"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14319"/>
    <dbReference type="HOGENOM" id="CLU_717935_0_0_1"/>
    <dbReference type="InParanoid" id="E3KVE0"/>
    <dbReference type="OrthoDB" id="2496660at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006044">
        <property type="term" value="P:N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022036">
        <property type="entry name" value="DUF3605"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35020">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-INDUCED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35020:SF2">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-INDUCED PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12239">
        <property type="entry name" value="DUF3605"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172233" evidence="1">
        <location>
            <begin position="25"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88235.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="389" mass="44369" checksum="B73212D9EE24E90F" modified="2012-04-18" version="2">MAFKLLSAFVALLFVFDRSLDVQAAIARHPSQVLSVAPAAEEAIPAGTVRMQALGITKRPFSDNIKVHDADEDKILEIANTPAKQLPSYEVPKTFDGLLSPVMQGNGVLFQRESKVQSKWHKFQTELRKKGISYEHHYAKQLKWDGPYPANKEPIMMDERQVKNEDWVKFERAQFPVKLPEDTEHVIAWLRAPLTTKECFKPVGKEIPPSDHEIFMANLEPYIEFINGHDVFGTNVDMKDEDKLQFAKDLMEVNDSLDLQEKLVHGEQEEKAAKAYRDAKNLENEQLLAGLEVDTSAAEKIDLPHVERAKEAMLWAVQNITRMIEAKYPGRRFVWFRVNRWIRTHHLNQIHIFLEKEPGTIEKAVRISGLKELKELANSHPGAGILAAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVZ9</accession>
    <name>E3KVZ9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">alpha-galactosidase</fullName>
            <ecNumber evidence="2">3.2.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_14052</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.</text>
            <dbReference type="EC" id="3.2.1.22"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.2.1.22" evidence="2"/>
    <dbReference type="EMBL" id="DS178314">
        <property type="protein sequence ID" value="EFP88474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332893.1">
        <property type="nucleotide sequence ID" value="XM_003332845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KVZ9"/>
    <dbReference type="GeneID" id="10540777"/>
    <dbReference type="KEGG" id="pgr:PGTG_14052"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14052"/>
    <dbReference type="HOGENOM" id="CLU_2211271_0_0_1"/>
    <dbReference type="InParanoid" id="E3KVZ9"/>
    <dbReference type="OrthoDB" id="2511907at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004557">
        <property type="term" value="F:alpha-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP88474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="107" mass="12205" checksum="747CC07E94634F95" modified="2011-01-11" version="1">MGWNSDFALDCHYELRDDDFTWQANQLAERGFNAAGYKTFVFDCGWEASSKDGTPGYLKRVFPNGPQEHASWLKWKGFNMGLGTWGGKINLTKDLIEHGSNEKLRLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KWD7</accession>
    <name>E3KWD7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_14817</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178315">
        <property type="protein sequence ID" value="EFP88612.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333031.2">
        <property type="nucleotide sequence ID" value="XM_003332983.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KWD7"/>
    <dbReference type="GeneID" id="10541240"/>
    <dbReference type="KEGG" id="pgr:PGTG_14817"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14817"/>
    <dbReference type="HOGENOM" id="CLU_2293069_0_0_1"/>
    <dbReference type="InParanoid" id="E3KWD7"/>
    <dbReference type="OrthoDB" id="2506773at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP88612.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="101" mass="10835" checksum="DFBD5938CD1EA69E" modified="2012-04-18" version="2">MASVPRLQIPFAIRKEGCANAEGLSKSGRTCSNDLGKVLLDMSLVAFWGMARLGKLVCDISHGQLEKDKGVWISDVRVASDAQSAWVKIHFAKTATGGETT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KYX4</accession>
    <name>E3KYX4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15241</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178322">
        <property type="protein sequence ID" value="EFP89399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333818.1">
        <property type="nucleotide sequence ID" value="XM_003333770.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KYX4"/>
    <dbReference type="GeneID" id="10547205"/>
    <dbReference type="KEGG" id="pgr:PGTG_15241"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15241"/>
    <dbReference type="HOGENOM" id="CLU_2224444_0_0_1"/>
    <dbReference type="InParanoid" id="E3KYX4"/>
    <dbReference type="OrthoDB" id="10288023at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173868" evidence="1">
        <location>
            <begin position="26"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="122" mass="13601" checksum="A9044F90CCE6BC5C" modified="2011-01-11" version="1">MKSTITQSLASLILLLLISCGFALCQVGTGYDAMCEKHNPPMLPYCFDYYKFLSITKQSFPDRNLLHSAGTFPSTERTADWEFSRGRPLFACPIGQWTLCCQRRNGHALLKTCDNAPKATGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZE8</accession>
    <name>E3KZE8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Amine oxidase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_15636</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178324">
        <property type="protein sequence ID" value="EFP89673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334092.1">
        <property type="nucleotide sequence ID" value="XM_003334044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZE8"/>
    <dbReference type="STRING" id="418459.E3KZE8"/>
    <dbReference type="GeneID" id="10545239"/>
    <dbReference type="KEGG" id="pgr:PGTG_15636"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15636"/>
    <dbReference type="eggNOG" id="ENOG502QSMW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028123_2_1_1"/>
    <dbReference type="InParanoid" id="E3KZE8"/>
    <dbReference type="OMA" id="RAWASWN"/>
    <dbReference type="OrthoDB" id="2503603at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.405.20:FF:000001">
        <property type="entry name" value="Amine oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1990:FF:000002">
        <property type="entry name" value="FAD-dependent oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.405.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050464">
        <property type="entry name" value="Zeta_carotene_desat/Oxidored"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923:SF17">
        <property type="entry name" value="AMINE OXIDASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42923">
        <property type="entry name" value="PROTOPORPHYRINOGEN OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP89673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="562" mass="63703" checksum="6F84B4939E4627ED" modified="2011-01-11" version="1">MYEFSENSGIFVTHIYRGFPGSCCNFLQLPPPFAILHTDSKTAGLVRMKVAVVGGGISGLTAVWLLNEYSDHEVELFEANEYVGGHTNTVTFKGTPVDTGFIVFNKLTYPNFVRFLEILNVDYIASNMSFSVKRTFAPAYEWSGNGISSLFNGISSWTDRISQLRLIWDLVRFNSLAVDVLAGPEGDLTIQEYLSKHGYSDCFKRNYLLPIISSIWSTPANKSAMHFPTKTLIRFMANHHLLQVFNQPQWLTIKNGSKTYVEKILSKLDPRKCFRSTPIESVSIDRSSKKVYLKASNSETLYGPYDQVIFASHADQTVKILKNSDCSDLFSSQIEILSRFQFNQNEAVLHNDLSMMPRNRSIWTSWNYLITDPSQNLDHPPPHPTKLATQEKAINESAVEKTVCLTYWMNLLQSIPEKTCGPVLVTLNPPEEIHPKWVFGRWKYDHPLYTSQSVKAQEEIATIQTKNGLSFVGAWTNYGFHEDGLTSTLRLLLVQHPSMFAVRSPFERVSVTEESIRPVGLGTRSLLGLIQASIQIFCRLVWLTRTIVYLLFPRTPVKPKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZN8</accession>
    <name>E3KZN8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Nucleolar protein 12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_15419</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
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    </reference>
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        <text evidence="1">Involved in pre-25S rRNA processing.</text>
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        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RRM RBM34 family.</text>
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        <property type="protein sequence ID" value="EFP89763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="GeneID" id="10545414"/>
    <dbReference type="KEGG" id="pgr:PGTG_15419"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15419"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
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        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4B/4H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23236:SF25">
        <property type="entry name" value="RNA-BINDING PROTEIN 34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RRM"/>
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    </dbReference>
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    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
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    <feature type="domain" description="RRM" evidence="9">
        <location>
            <begin position="278"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
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            <begin position="205"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
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            <end position="275"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="374"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="492"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
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            <begin position="11"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
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            <begin position="39"/>
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        </location>
    </feature>
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        </location>
    </feature>
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            <end position="521"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002475"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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        <source>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP89763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="521" mass="57286" checksum="01200B0394176259" modified="2011-01-11" version="1">MAKEPTPATMTDKKKTTKESEPTPKILHETHQKSKDKNAASSVGPSSSKSAESFEEKNRRTVFVGNVHIDCVKNKSASRALLEHLLNPSKEESGGLAPQARIDSIRYRGIPLATPIGSEPPKDQHGSKRSKAWQDSQAAGGTRPSFDDAAGGSAGRRGAKPLESSESTLPTVKFLTGGQKRKIGYVTGDIHPEAKSCLAYVVIAPPPPPPPSSSTTTSSSHPSAHLSAIELAQLIVAKSDGTSFMDRVIRCDMASQPPSKKTPNNPSSHTIDNKEQRRTLFIGGLDFVEEEDSIRKAVESKLVEEKKGLPEGAATWVERVRVVRDKATSLTKGFAYVLLRTQDAVEEMLALPEGSFKIGKRRVRLQKYLSSGQSSALKRVREPTTKNGEKRGGKPNPLNPSQDSTKRPRIDLTKEIQTTYQGPDQSQELALLDKSERKKIKSANQARVERRMLKKQNQLKIKILNSQKSSHKKQEFHNTLKPFKKSVVHKLASIKDTKNNNKNKAFKNKNKSKVESKSSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3L0C5</accession>
    <name>E3L0C5_PUCGT</name>
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        <recommendedName>
            <fullName evidence="1">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16192</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP89904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="KEGG" id="pgr:PGTG_16192"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16192"/>
    <dbReference type="HOGENOM" id="CLU_1482708_0_0_1"/>
    <dbReference type="InParanoid" id="E3L0C5"/>
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    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="3"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="182" mass="20812" checksum="16B98348B2B73D5E" modified="2011-01-11" version="1">MPPEEYEEIKFLTTTLVQLGHYYNNQSNSSLLEGVMKGFGWRKGYGQDMEHFGTYTPAKVKTEEDYRKWRALLLQLPMVESIISRRFQKLAPGAFHQSVEKMKSAKIPSFASPEFDQVPSLPFASNLTATWGEFYNRAHIDNNTGQTIGGFSARDAQVRRLIQGFLNPPFKLHYLPMGTLDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3L0D4</accession>
    <name>E3L0D4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15880</name>
    </gene>
    <organism evidence="2 3">
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        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178328">
        <property type="protein sequence ID" value="EFP90032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334451.1">
        <property type="nucleotide sequence ID" value="XM_003334403.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L0D4"/>
    <dbReference type="GeneID" id="10545929"/>
    <dbReference type="KEGG" id="pgr:PGTG_15880"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15880"/>
    <dbReference type="HOGENOM" id="CLU_044627_1_0_1"/>
    <dbReference type="InParanoid" id="E3L0D4"/>
    <dbReference type="OrthoDB" id="10278074at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="451" mass="49218" checksum="447641E914B0C693" modified="2011-01-11" version="1">MSQPHDRVTPSQSPAAVTPRNRGRLPLAHVPPNNRFHPHGGSANRSRGSTRGRVSNRGQASNRGHSVSQGGGLVPATPEGGRPQWPNNEGHESANSGWAGATFPPPDPVLDPALNWLGESQPSPTSENFINPGGSNSEGSSDHEDRSSYDPLPDAPALTWGAAPPPPNQETADTAVGGSLVGELQGRLHLSESNLALANRLFRATESEKWGLTVLMFVHLLGKTSDATVSNPLSAPTPEAKNFTFSTHLKAYVQSQSPSFHKDYLPPGYPRDISASAKVLKLMRGLLKTEKCLLRTLLLHNIKEHNHRPIDGAVPDLDGLVLIIDTFMAARKQARTVADILRSYPASVRTRLAFLRLYVVVHLIHRDPKENISQWELIDQQLEYVKGQSELYRIAYGRVVEAIDKELFGHGVKFEDMDHEDIRVPTDEDVEEEIRVMSASGGSTGESSPFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3L0P9</accession>
    <name>E3L0P9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15951</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178329">
        <property type="protein sequence ID" value="EFP90103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334522.1">
        <property type="nucleotide sequence ID" value="XM_003334474.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L0P9"/>
    <dbReference type="GeneID" id="10546081"/>
    <dbReference type="KEGG" id="pgr:PGTG_15951"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15951"/>
    <dbReference type="HOGENOM" id="CLU_1587321_0_0_1"/>
    <dbReference type="InParanoid" id="E3L0P9"/>
    <dbReference type="OrthoDB" id="2513615at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="178" mass="19212" checksum="916DE1A314C00FDE" modified="2011-01-11" version="1">MGHNPNRHAVVRPAPPNSAPAVVSTFPRSPPLSAFPAASPPFHLPSRLRPTAQVTRSNAIRRPVATHLNDIDINGDLVNKALGLTPTSPAPPLPLASIPPMKTSSVDYSTLPLPPLPLPPTPPPAAPLLPLLPFPRPPRRARVNSNRHAMIILVPQELPQELRDIQLDAWMTHDDDST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3L1F4</accession>
    <name>E3L1F4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Acyl-protein thioesterase 1</fullName>
            <ecNumber evidence="2">3.1.2.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Palmitoyl-protein hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_16139</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Hydrolyzes fatty acids from S-acylated cysteine residues in proteins with a strong preference for palmitoylated G-alpha proteins over other acyl substrates. Mediates the deacylation of G-alpha proteins such as GPA1 in vivo, but has weak or no activity toward palmitoylated Ras proteins. Has weak lysophospholipase activity in vitro; however such activity may not exist in vivo.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>S-hexadecanoyl-L-cysteinyl-[protein] + H2O = L-cysteinyl-[protein] + hexadecanoate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19233"/>
            <dbReference type="Rhea" id="RHEA-COMP:10131"/>
            <dbReference type="Rhea" id="RHEA-COMP:11032"/>
            <dbReference type="ChEBI" id="CHEBI:7896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:74151"/>
            <dbReference type="EC" id="3.1.2.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily. AB hydrolase 2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.2.22" evidence="2"/>
    <dbReference type="EMBL" id="DS178332">
        <property type="protein sequence ID" value="EFP90379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334798.1">
        <property type="nucleotide sequence ID" value="XM_003334750.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L1F4"/>
    <dbReference type="STRING" id="418459.E3L1F4"/>
    <dbReference type="GeneID" id="10530273"/>
    <dbReference type="KEGG" id="pgr:PGTG_16139"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16139"/>
    <dbReference type="HOGENOM" id="CLU_866363_0_0_1"/>
    <dbReference type="InParanoid" id="E3L1F4"/>
    <dbReference type="OMA" id="LMFRTYN"/>
    <dbReference type="OrthoDB" id="2506865at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052689">
        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008474">
        <property type="term" value="F:palmitoyl-(protein) hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000716">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050565">
        <property type="entry name" value="LYPA1-2/EST-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003140">
        <property type="entry name" value="PLipase/COase/thioEstase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655:SF17">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10655">
        <property type="entry name" value="LYSOPHOSPHOLIPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02230">
        <property type="entry name" value="Abhydrolase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0719">Serine esterase</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipase/carboxylesterase/thioesterase" evidence="11">
        <location>
            <begin position="93"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP90379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="321" mass="36200" checksum="6A993827C2413B21" modified="2011-01-11" version="1">MTPGIHTKPSLRATLIGAGVMASLMLFHLYLEYQPSLFLLNHDLRGGTRVIQDPRSPRTLLNESSPARGWLEVEYPAGPIEPLEYTRLPAIKGPNNWTCLLLHGLGDHQASDSFRLREALLTLRPALFEPMSFVIPAADALPVTVFPGLPRPAWFDIRNWSDIHRDEDVLHMENNVRRLVHIINLLQLDLSRTIIAGFSQGAVMSLLLGLTLDRPPAGVIMLSGFLPMPDQLEAIFSRPITETGIRRQTILHWLHGAEDPYFPLPFAQQGFRRLQKLALFPPHNLFFSHINRLDHRWSLEELGLLANSSLDRFFPTAPSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3L1H4</accession>
    <name>E3L1H4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Enterotoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_16159</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178332">
        <property type="protein sequence ID" value="EFP90399.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334818.2">
        <property type="nucleotide sequence ID" value="XM_003334770.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L1H4"/>
    <dbReference type="GeneID" id="10530293"/>
    <dbReference type="KEGG" id="pgr:PGTG_16159"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16159"/>
    <dbReference type="eggNOG" id="ENOG502QRJE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017366_1_0_1"/>
    <dbReference type="InParanoid" id="E3L1H4"/>
    <dbReference type="OrthoDB" id="10261782at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038921">
        <property type="entry name" value="YOR389W-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35204:SF1">
        <property type="entry name" value="ENTEROTOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35204">
        <property type="entry name" value="YALI0A21131P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173932" description="Enterotoxin" evidence="2">
        <location>
            <begin position="29"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="373"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP90399.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="625" mass="71158" checksum="B237B8555AA0F19C" modified="2012-04-18" version="2">MFAFEALTTRRLTLILLAGLTAFPRPNAIIADESDQLSFSPKNSETESSQLPPGRDHPNYIFASFAGLLQQWPNTFAYSGHSIIPGVVPRGTLLYHGTNHRTPPPSEGLEWLAFNPEYSFAIHSHRLGQVDLYKYTATRALRIIYFDGQSSSLGTPGFMDSQSVLIHGTVHKHFGDGGRYIKADYDRAAELCKIGKKWGFEGVVRMNTCFEVLWCDFTEGVELLGGTNTTDPFDTHYEPGEFTESMKVTELHATDSSSDSSEIFVQNRSDAVNPLFPDEVGKNFRQRIEVFGAINSTVRVKNDDDSSENENPEESLLQVLTRPRVMNSPFYSRSPLYYFRAASRQFFMPGEVRVNLDPSGFVSFYDRIESLSQKRRDDGTDDGPRHTHRLHGISPSDVKKIEDRLFNVLARKNAENWQTDSDRLDWRALVWTIVQTYSKPLAELDYLLKRNDLNAINQAAEVRGLTYGMILPHIDFSSWNISDPTWVDRNIKKCARGFTSGTYRVSDLTESIEVIIGAIEGTLDRLCSTIFSIFSQTIELVIPIDSLIRPDSKLERQAKSRIIEWQQKTEELVKWLQWSTRAHCDPPCKPDEMCLPPLWPAYWMKGLPQIEDQFPVCSKDSWKTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3L2A8</accession>
    <name>E3L2A8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16721</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178336">
        <property type="protein sequence ID" value="EFP90695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335114.1">
        <property type="nucleotide sequence ID" value="XM_003335066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L2A8"/>
    <dbReference type="GeneID" id="10530595"/>
    <dbReference type="KEGG" id="pgr:PGTG_16721"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16721"/>
    <dbReference type="HOGENOM" id="CLU_044243_0_0_1"/>
    <dbReference type="InParanoid" id="E3L2A8"/>
    <dbReference type="OrthoDB" id="2508363at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="91"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="202"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="355" mass="39883" checksum="0A3C0DF24AA04CB9" modified="2011-01-11" version="1">MVELEKTAKLIQYYLTLLASEKAQRAADYQPSDWELLTPDEQRARALKRVEIREEERIANLLLWNSVKRDHKAKHRLLLTELRDKRLARMSATSNSSAATTPAAGTTPVAETAANSPNTGRTIQTSTAPYPQPLDRGAANQLEERVNRLKLITIQKKMAPVLVKKQTEATLAENGSTPAVETPATARMTQTAAKTTTSSVEKAPDAMDIDPRASSSSADIQQRPKSPEVRILTKEERIQLLVKEHIVLWKKFEADLPLGASEGNRALLNRAQESQRTLQKLIPRAEVEEYVKGWNPWTAKRDLFPVLQQEGGGKKRSSSSRANFSYKDAKMNDPRRWKKLMKACHTLESGYMNMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3C9</accession>
    <name>E3L3C9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">DNA polymerase epsilon catalytic subunit</fullName>
            <ecNumber evidence="19">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">PGTG_17326</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="17 19">DNA polymerase II participates in chromosomal DNA replication.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 19">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="18">Heterotetramer. Consists of 4 subunits: POL2, DPB2, DPB3 and DPB4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 19">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="19"/>
    <dbReference type="EMBL" id="DS178340">
        <property type="protein sequence ID" value="EFP91054.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335473.2">
        <property type="nucleotide sequence ID" value="XM_003335425.2"/>
    </dbReference>
    <dbReference type="FunCoup" id="E3L3C9">
        <property type="interactions" value="344"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L3C9"/>
    <dbReference type="GeneID" id="10528552"/>
    <dbReference type="KEGG" id="pgr:PGTG_17326"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17326"/>
    <dbReference type="eggNOG" id="KOG1798">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000556_0_1_1"/>
    <dbReference type="InParanoid" id="E3L3C9"/>
    <dbReference type="OrthoDB" id="10060449at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008622">
        <property type="term" value="C:epsilon DNA polymerase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008310">
        <property type="term" value="F:single-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006287">
        <property type="term" value="P:base-excision repair, gap-filling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045004">
        <property type="term" value="P:DNA replication proofreading"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006297">
        <property type="term" value="P:nucleotide-excision repair, DNA gap filling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05779">
        <property type="entry name" value="DNA_polB_epsilon_exo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05535">
        <property type="entry name" value="POLBc_epsilon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000002">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.690:FF:000005">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.10:FF:000010">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1600.10:FF:000006">
        <property type="entry name" value="DNA polymerase epsilon catalytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013697">
        <property type="entry name" value="DNA_pol_e_suA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029703">
        <property type="entry name" value="POL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055191">
        <property type="entry name" value="POL2_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054475">
        <property type="entry name" value="Znf-DPOE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10670">
        <property type="entry name" value="DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10670:SF0">
        <property type="entry name" value="DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08490">
        <property type="entry name" value="DUF1744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22634">
        <property type="entry name" value="POL2_thumb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22912">
        <property type="entry name" value="zf-DPOE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23250">
        <property type="entry name" value="zf_DPOE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01159">
        <property type="entry name" value="DUF1744"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 19" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 19" id="KW-0235">DNA replication</keyword>
    <keyword evidence="14 19" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="11 19" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="12 19" id="KW-0408">Iron</keyword>
    <keyword evidence="13 19" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 19" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15 19" id="KW-0539">Nucleus</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 19" id="KW-0862">Zinc</keyword>
    <keyword evidence="9 19" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="DNA polymerase epsilon catalytic subunit A C-terminal" evidence="21">
        <location>
            <begin position="1546"/>
            <end position="1947"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="20">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="20">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="20">
        <location>
            <begin position="51"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00057054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00065544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU365029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="SMART" id="SM01159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="EFP91054.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="2267" mass="256897" checksum="295A1EAC4256AD5A" modified="2012-04-18" version="2">MAPFKPRGRGSYSSFRGRGRGGYKPGGGRGGGTKGPGGVNRTADGLVLENDQDREGTESLEKWNRAQQDREIDESLGFVRFDQGQPRVGWLINIQPTLVQEALGPQTDKAEGPSQPSAAVDLYFLQDDGSMFRSTLIYHPYFLIASRVGKEAEVEEWIRKKYDGQVMKVEGVIKEDLKLPNHLAGHKRRFLKLVFRNVDQLLRVRRDLLAVALSNNAKMDALDSYAEVVKAGRGEFDGMDIEDFDSLDFNGVQDTLGGTASRKGKLRSLVSKSNSLQPAATLPSEWIVDIREYDIPYYLRVAIDKNLRVGLWYTVAIEQNNVTCTIIPERVERAEPVVMAYDIETTKAPLKFPDSANDQIMMISYMIDGQGFLITNREIVADDIADFDYSPKPEYEGQFTIFNEENELALIRRWFDHIRDARPTVMATYNGDSFDFPFVYARAAIHGLDMFKEIGFNPDSESEFRSRTCIHMDCFKWVKRDSYLPQGSQGLKAVTVAKLGYNPIELDPELMTPYAVEQPQILAQYSVSDAVATYYLYMKYVHPFIFSLCNIIPLNPDEVLRKGSGTLCETLLMVEAFNSSIIMPNRHTDPHGTMYNGHLLESETYVGGHVEALEAGVFRSDILSDFKLVPSRCQQLIDELDSALKFSIVEEGQYALEDVVNYDDVRAAIQEALETMRDEPDRQEFPLIYHLDVAAMYPNIMLSNRLQPDSVVSEGMCAACDYNRPNKTCDRRMQWAWRGEYFPAKLDEYKMIRNAIESEVFPSKKPGQPPRKFGDLAPAEQTTLLHKRLGDYSRKVYRKTHETTIEEKTAIICQRENPFYIDTVRAFRDRRYTYKGLHKTWKKKADQAQQEGRSVGEIAEAKKMIVIYDSLQLAHKCILNSFYGYVMRKGARWHSMEMAGITCLTGARIIQAAREIIEEVGRPLELDTDGIWCMLPKIFPDNFTFKLTNGKTHAISYPCSILNHLVHQRFTNDAYHQWNPETKTYDVRNENSIFFELDGPYRAMILPSSKEEDKLLKKRYAVFNPDGSLAELKGFEVKRRGELQLIKIFQTSIFEKFLVGDDLKACYDAVAQVANRWLDVLATKAISMHDEELIDLISENRSMSKTLAEYGAQKSTSIRTAKRLSEFLGNQMVKDKGLSCRFIISAKPHGSPVTDRAVPVAIFSAEPSVQKHFLRKWLKDNSLVDLSLRSILDWGYYTERLGSVIQKLITIPAAMQKVLNPVPRIHHPDWLARRLATREDTFKQHKIDDMFARQAPLSAEDLEALRAVKQKKADALARKEPPSLPPLPKPSFHIDYSGWIAAMKPIWRAQRTAKKATSGSKFVPGSIASMLHVQNLKVVSHSSHLELVQLSPNPLKPGEFQAWVLFNDVLQPARITVLREFYVNLKHEPGPDFFPESCMAEPIVKTLPRSQARINLFKISVTEKSYIEDASYYQKMVTSPEVEGVYERQVPLLQRALIRLGATCSLSSQSRLSLVDGIDKGFDLSDLTSLEQQVSQRRYLDKGKAIRYVLVYHFQSGSRHAVGIFNTNAAAQIFITDRSSAPQVPNIQKYYKNVLNSRLAIMEKEPGVFEYDPNMLVEVTNVATETLALKAISRELALLKGQKTGPGYVLMIHSMKDLSYYQEKVLSVSKYPVVMVHSSQADNKFEAFGWQRFAAERMVQHYVRASSFLRERVAQAEQFDVPVANFEMDAPRSLMDLEFARRLTKADHLIWWSASRKPDLGGKEADLNTELVMDVLSNPEISVMGAYSDASVELTIQHLAMNSVLQSSWVYEISGGTGGSLGFQDASHNLDDYSKGSVASSLLGDSDLPVQTFTLFRTMVRAWIAESARAGTGHHYVGLIKHFWRWLSSSSSSMFDPALYRFVHGLMKKVFSQLLAEFRRLGTRIIYADFGRIFLLTSKPNSASALAYASYIVTAINGRDLFKFLELDIVRFWEQLLWVDATNFAGITCGDPLKASDSEVVENIDMNWNMVNFLPPALQQDFLHVVCLYVYDLHQCHGQASSNDQIQLEVDRKQIEAQLLRSLVNSKITRQTLKIVNQIIERQNKAAADDELRTTFDFPRLPGSHIAMKKPVLEFVKMVCFVMELATLVTAEVRIMKRVLLDLIGVREFSNEAVFKNPCIPFKIPDTICRHCSAVRSFDLSRDSDLLASASTRRVGQPPEWLCGHCGQSYDRLGIEAMLIEQLHKQVASFQLQDLRCSRCAQLKEDTMSLRCPCSGEYILAGDAGSQADRPSSAHLERRLAVARSVAEAHQLDVLLEFVQWLNSQIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3M9</accession>
    <name>E3L3M9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Acyl-CoA dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_16899</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178342">
        <property type="protein sequence ID" value="EFP91154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335573.1">
        <property type="nucleotide sequence ID" value="XM_003335525.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L3M9"/>
    <dbReference type="STRING" id="418459.E3L3M9"/>
    <dbReference type="GeneID" id="10528816"/>
    <dbReference type="KEGG" id="pgr:PGTG_16899"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16899"/>
    <dbReference type="eggNOG" id="KOG1469">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_1_2_1"/>
    <dbReference type="InParanoid" id="E3L3M9"/>
    <dbReference type="OMA" id="WNMFLSK"/>
    <dbReference type="OrthoDB" id="434771at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033539">
        <property type="term" value="P:fatty acid beta-oxidation using acyl-CoA dehydrogenase"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000002">
        <property type="entry name" value="Acyl-CoA dehydrogenase fadE12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050741">
        <property type="entry name" value="Acyl-CoA_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083:SF13">
        <property type="entry name" value="ACYL-COA DEHYDROGENASE FAMILY MEMBER 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083">
        <property type="entry name" value="MEDIUM-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0274">FAD</keyword>
    <keyword evidence="4 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="10">
        <location>
            <begin position="32"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="9">
        <location>
            <begin position="156"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="8">
        <location>
            <begin position="271"/>
            <end position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP91154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="455" mass="50812" checksum="20B273658D28909B" modified="2011-01-11" version="1">MTSHPITRFIPQSTWHLVEPHFTSFGKEKLEKVIEFVENECIPAESIYHAQISSDPQRRWSTVPTIMEDLKSKARSRGLWNLFLSKAHYPELGVPLTNLEYAVMAEVMGHAIRIAPESMNCSAPDTGNMEVLARYGTPYQKEKWLKPLMNGQTRSSFAMTEPAVASSDATNIQTSIMYDKKTQQIVINGRKWWISGAGDPRNAIHLVMGKSDTSAPKHSQQSIVIVPPNTPGVKLIRPMQVFGYDDAPEGHFEVLYEDVRVPIENLVYDWGKGFEIVQSRLGPGRIHHCMRSIGIAERAISLMLLRVTDPRKMTFGKQLYQHGATAKEIAYSRIELDGIRLLVYSAAHQIDLVKAKGAMKSIGMAKAQVPKVVNAIIDRAIQVHGGEGVSQDQPLAAMFAAVRTLRMADGPDEVHEAQVAQMELKRVPLLRQQLEAKTEAEKALRVQYQIGGSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>E3L508</accession>
    <name>E3L508_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Multifunctional tryptophan biosynthesis protein</fullName>
            <ecNumber evidence="8">4.1.1.48</ecNumber>
            <ecNumber evidence="7">4.1.3.27</ecNumber>
            <ecNumber evidence="9">5.3.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">PGTG_17687</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Trifunctional enzyme bearing the Gln amidotransferase (GATase) domain of anthranilate synthase, indole-glycerolphosphate synthase, and phosphoribosylanthranilate isomerase activities.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+) = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:23476"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="EC" id="4.1.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:21540"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="EC" id="5.3.1.24"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.48" evidence="8"/>
    <dbReference type="EC" id="4.1.3.27" evidence="7"/>
    <dbReference type="EC" id="5.3.1.24" evidence="9"/>
    <dbReference type="EMBL" id="DS178349">
        <property type="protein sequence ID" value="EFP91633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336052.1">
        <property type="nucleotide sequence ID" value="XM_003336004.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L508"/>
    <dbReference type="FunCoup" id="E3L508">
        <property type="interactions" value="92"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L508"/>
    <dbReference type="MEROPS" id="C26.959"/>
    <dbReference type="GeneID" id="10531570"/>
    <dbReference type="KEGG" id="pgr:PGTG_17687"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17687"/>
    <dbReference type="eggNOG" id="KOG0026">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4201">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4202">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007713_2_0_1"/>
    <dbReference type="InParanoid" id="E3L508"/>
    <dbReference type="OMA" id="EPIEWAN"/>
    <dbReference type="OrthoDB" id="524799at2759"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004425">
        <property type="term" value="F:indole-3-glycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00331">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00405">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000136">
        <property type="entry name" value="Multifunctional tryptophan biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000031">
        <property type="entry name" value="Multifunctional tryptophan biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00135">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050472">
        <property type="entry name" value="Anth_synth/Amidotransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013798">
        <property type="entry name" value="Indole-3-glycerol_P_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001468">
        <property type="entry name" value="Indole-3-GlycerolPSynthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001240">
        <property type="entry name" value="PRAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00566">
        <property type="entry name" value="trpG_papA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418:SF4">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00218">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00697">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00614">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="14" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="13" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="15" id="KW-0413">Isomerase</keyword>
    <keyword evidence="16" id="KW-0456">Lyase</keyword>
    <keyword evidence="17" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="19">
        <location>
            <begin position="14"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Indole-3-glycerol phosphate synthase" evidence="20">
        <location>
            <begin position="250"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="domain" description="N-(5'phosphoribosyl) anthranilate isomerase (PRAI)" evidence="21">
        <location>
            <begin position="618"/>
            <end position="806"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00004873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00012362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00012572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00018819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00047683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="Pfam" id="PF00218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF00697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="EFP91633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="813" mass="88268" checksum="E25A93DAEB3961C6" modified="2011-01-11" version="1">MTKTSLLAGEVKVLIIDNYDSFTWNLYQYLCLLGASVSVIRSQACSLDELVASYPKLTHLVISPGPGHPSTDSGISIPAIHHYAGKIPILGICMGLQCIYSAFGGEVTKVGEIVHGKTSLVSHDGKGLFKALPAHIQCTRYHSLAGRFGTLPSELEVTCWTNSSAELANDPGPFDDQSSIIMGVRHKLLTIESVQYHPESILSEEGKPFLLNFLNLRGGHWSENPDFQVSPTPKSVPIASAESSSKQTILETICLQRAKDLIEAKCIPGLSPTDLKRKLAANVPPVQIDFYERIRRPTTGKVALMAEIKRASPSKGSFMTPTTPSSPAIAVSYAAAGASVISVLTEPKWFKGTLLDMLEVRLSVENVPNRPAILRKDFIIDPYQIDEARCYGADTILLIVACLSLDQLRLLYNHAKSLDMEPLVEVANAAELELALQIKARVIGVNNRNLHDFRVDMNTTSKMLDELNARSSAQNGSSNEIIMCALSGISSRSDVDRYAAEGVGAVLVGEALMKAEDKVAFVHELLGIPPTPKVTTRKPLVKICGIKTVEAAVHATNAGADLLGLIFVPKSKRCVTPQQADAIIKAVRKSSESSPKPSLETEEIDLDTTDWFSLQSSRLLQKRRTQGKPLIVGVFQNSSLSEIEKVLNQLGDSIDLIQLHGSEDFRMSKFLSKPVIKAFHIEVERGTTDAGEEVFQEGFRKTLSMINEPGFHSFSILDSSNLSQCGGTGQSFNWNAVKAVLASSATSSSTTHRNLVIAGGLNAQNVATCIQTFNPFILDICSGVEVENCQNGVVENKDFDKISQVIKLIKSDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3L5T2</accession>
    <name>E3L5T2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17934</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178354">
        <property type="protein sequence ID" value="EFP91907.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336326.2">
        <property type="nucleotide sequence ID" value="XM_003336278.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L5T2"/>
    <dbReference type="GeneID" id="10531854"/>
    <dbReference type="KEGG" id="pgr:PGTG_17934"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17934"/>
    <dbReference type="HOGENOM" id="CLU_970231_0_0_1"/>
    <dbReference type="InParanoid" id="E3L5T2"/>
    <dbReference type="OrthoDB" id="10447004at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91907.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="287" mass="31549" checksum="53FE3BF13B2A784A" modified="2012-04-18" version="2">MNSTKFFDFFGKIREIIVILADIKSGTGNLITRLRKRPVHYLVVFLAGGAILDILLTQTSNSKSLLVVACGMAADYIAFWTLHSTNPILWPLWRTSKEAKEMPNEVTSPMMYLFNGKGDLPAKLGEETPTSNKAGKCHSDDDISQSSNSPSENLDTKPTSKPTQSPPPHPTNSNTDQLMQAIQKLLDETELPLASPLIAQLCSVSPTDGLTNEDLDTMFFMNVNSTMDALFGIEMGTTHITGGEGGLKLVLSWLDRARAHPSWAPAHDCLLDIKLERILDALKDYNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3L6K5</accession>
    <name>E3L6K5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178359">
        <property type="protein sequence ID" value="EFP92180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336599.1">
        <property type="nucleotide sequence ID" value="XM_003336551.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L6K5"/>
    <dbReference type="GeneID" id="10537507"/>
    <dbReference type="KEGG" id="pgr:PGTG_17910"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17910"/>
    <dbReference type="HOGENOM" id="CLU_1563207_0_0_1"/>
    <dbReference type="InParanoid" id="E3L6K5"/>
    <dbReference type="OrthoDB" id="2498139at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174432" description="Secreted protein" evidence="1">
        <location>
            <begin position="29"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="173" mass="18440" checksum="A84ED5C6FED6C4B7" modified="2011-01-11" version="1">MHLHTDSPFRIILSAFLAAFCCAISSWAATIMPNGAALGDMCDNSLACSVIDETWPKNKTFSCASKSITPTALGTATQALTIGKPRKIENGTSWPASASSGCANNGSDYSKHSYSNGLWTMYYTIRTVCKCKGKTRDAMAMPMCRAATSQDVSACNLAIFKFCSVEMNGSPCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3L6Z2</accession>
    <name>E3L6Z2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_17991</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP92317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003336688.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L6Z2"/>
    <dbReference type="STRING" id="418459.E3L6Z2"/>
    <dbReference type="GeneID" id="10537978"/>
    <dbReference type="KEGG" id="pgr:PGTG_17991"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17991"/>
    <dbReference type="eggNOG" id="KOG4225">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_1235572_0_0_1"/>
    <dbReference type="InParanoid" id="E3L6Z2"/>
    <dbReference type="OMA" id="IFNCAIC"/>
    <dbReference type="OrthoDB" id="2497747at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:002749">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051864">
        <property type="entry name" value="NCF2_NOXA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15175">
        <property type="entry name" value="NEUTROPHIL CYTOSOLIC FACTOR 2, NEUTROPHIL NADPH OXIDASE FACTOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15175:SF0">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING PROTEIN C23A1.17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="1">
        <location>
            <begin position="37"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="200"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="222"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP92317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="260" mass="29621" checksum="BD443C57E4B975EE" modified="2011-01-11" version="1">MNYQNHTYRTWMRALKYFDDQDLDRSLYVFEMISRSPKIFFNLGMIQTGLGDYQSAVDCFQAAVTDESASVVEYFQLGAILFSLEEYESAHKNFSIAFLLMGDRSHIDCQEAGLNYCLYSCEILFNRAMCLLHLGKFDEGTETLSDAREKQEYPSQIPCSLEVFSIPALREDGSYGSPRRADYWTVFQVSNGTLYCPVPQASDRMSQSSDYKLESIPSSPTESMPSITGESSSSESHSSARKSPFKLFKIVKRRSPHLFK</sequence>
</entry>
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    <accession>E3L9J6</accession>
    <name>E3L9J6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19167</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178386">
        <property type="protein sequence ID" value="EFP93221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L9J6"/>
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    <dbReference type="KEGG" id="pgr:PGTG_19167"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19167"/>
    <dbReference type="HOGENOM" id="CLU_2265037_0_0_1"/>
    <dbReference type="InParanoid" id="E3L9J6"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="103" mass="11377" checksum="093733777AE73319" modified="2011-01-11" version="1">MQCQLGIDELFAIDVGIQPAHSLSLHFALACPQNLIHIESSFLKLDQTTQALATFALVDPVVIGYLWILLHHHTVRTAAIGFPPGYRGIHNAYTPSPRCPHCH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3LAC9</accession>
    <name>E3LAC9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178395">
        <property type="protein sequence ID" value="EFP93504.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337923.2">
        <property type="nucleotide sequence ID" value="XM_003337875.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LAC9"/>
    <dbReference type="GeneID" id="10543136"/>
    <dbReference type="KEGG" id="pgr:PGTG_19480"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19480"/>
    <dbReference type="HOGENOM" id="CLU_046891_0_0_1"/>
    <dbReference type="InParanoid" id="E3LAC9"/>
    <dbReference type="OrthoDB" id="2506425at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93504.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="298" mass="33429" checksum="A396E00884805FA0" modified="2012-04-18" version="2">MSSTTQDSDVASHPTLKITGVEQLKPPGSESNYLDWSWILDIHFTATDVDYVIHDKPEDAKAKPNWARDNKAVCGVISRTIHPTNIRNVRHLKNDARGLWDALKRAHQDSSAGGVTYWLRKLTSSRMVNDDLSAHLEDMAKTFERLSSLVTSEAPLTPNDIYSSSILTSLPTDWLACVSSMMNEPRVDPDRVIDALKAEELRRKTRSSDQPIVESVSQASNKKSSKSKSAKQDQPTRHCTFCNLDGHDLNRCFNVARLIENHKLNQAPPKNKKVSRSELRSSRPPGTQDQNTYDIILA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3LAE9</accession>
    <name>E3LAE9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18938</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178396">
        <property type="protein sequence ID" value="EFP93524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337943.1">
        <property type="nucleotide sequence ID" value="XM_003337895.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LAE9"/>
    <dbReference type="GeneID" id="10543639"/>
    <dbReference type="KEGG" id="pgr:PGTG_18938"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18938"/>
    <dbReference type="HOGENOM" id="CLU_1046391_0_0_1"/>
    <dbReference type="InParanoid" id="E3LAE9"/>
    <dbReference type="OMA" id="MESKFIH"/>
    <dbReference type="OrthoDB" id="2516766at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="245"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="266" mass="29732" checksum="193125EBA389FD83" modified="2011-01-11" version="1">MSYQTSSAHDSAMDTGSSTPSTNAPSTVEITNTLRSLELCIAGLQERTEGAVQPIAELLHKIEEELGNIQEQLAHVTHPSQGSGTTERNAERNATNIFNPPEMPVERITTFNRYIPVFLAERVPAAQVHYIEALNNLSFEDKLIMSSVTIERNELYQDAYLALLQVESELLKESIASGAIAANITNRDSFLYELFHIFNDSPEHKRAQDSVLRLRLKRYRVKEIADTINTFYMATTNEEEAVVRDEEGAVAENESGTVARNEDGDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3LAS1</accession>
    <name>E3LAS1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hydrophobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19595</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178401">
        <property type="protein sequence ID" value="EFP93646.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338065.2">
        <property type="nucleotide sequence ID" value="XM_003338017.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LAS1"/>
    <dbReference type="GeneID" id="10534935"/>
    <dbReference type="KEGG" id="pgr:PGTG_19595"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19595"/>
    <dbReference type="HOGENOM" id="CLU_2400724_0_0_1"/>
    <dbReference type="InParanoid" id="E3LAS1"/>
    <dbReference type="OrthoDB" id="10284388at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173254" description="Hydrophobin" evidence="1">
        <location>
            <begin position="26"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93646.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="93" mass="10023" checksum="E68FBA09B1D366F5" modified="2012-04-18" version="2">MQSVIAILASSILMLLISGPMHAAAKNMICPNRDNSNGYCVQMDTGAPIIPKIRNIPGGMFPTFTCASDYLALCCPSIPVKDLKQCKFASSWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3LB44</accession>
    <name>E3LB44_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19796</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178408">
        <property type="protein sequence ID" value="EFP93769.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338188.2">
        <property type="nucleotide sequence ID" value="XM_003338140.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LB44"/>
    <dbReference type="GeneID" id="10535139"/>
    <dbReference type="KEGG" id="pgr:PGTG_19796"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19796"/>
    <dbReference type="HOGENOM" id="CLU_186283_0_0_1"/>
    <dbReference type="InParanoid" id="E3LB44"/>
    <dbReference type="OrthoDB" id="10276532at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93769.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="93" mass="9525" checksum="CD1F55594AF6F0FD" modified="2012-04-18" version="2">MTQALPNTMVCVALAPNASTDDKYSCDGVKIQGTTAQGTACCSPGTSPKKADPEKDPQSSPQSPLSGMTEEKYKSVCGKADDPAPSSTTEADP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3LC08</accession>
    <name>E3LC08_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19986</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178437">
        <property type="protein sequence ID" value="EFP94083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338502.1">
        <property type="nucleotide sequence ID" value="XM_003338454.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LC08"/>
    <dbReference type="GeneID" id="10535775"/>
    <dbReference type="KEGG" id="pgr:PGTG_19986"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19986"/>
    <dbReference type="HOGENOM" id="CLU_283440_0_0_1"/>
    <dbReference type="InParanoid" id="E3LC08"/>
    <dbReference type="OrthoDB" id="2507860at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="510"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="256"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="279"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="305"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="336"/>
            <end position="346"/>
        </location>
    </feature>
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        <location>
            <begin position="534"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="559"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="586"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="608"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="669"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="725"/>
            <end position="738"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP94083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1100" mass="119549" checksum="72EFEF3DF01CF342" modified="2011-01-11" version="1">MAYRNKQPRHLNFSGLLAKHRIDNPNIILESQRGEHHSQSAAVYPQLGGGATTNTTDTAPSNFESIAEDLYPNIIETPAIKDDPTATVLYSTPKPAGTLSVNTSSRNLAQSIISRRTKTNPPRQFSVDSNHSSILLSHSPSSIDPPFAVCQSPTSQRLAFPGNPKRGYSTATVKFATPDRDLDSKTIGHSPRGLRSKLSYSEHESNPLIDPTPSLSGSSPSNSRSYRSNHSSLSTPASDIRPSLYSISPQNYCKKSPTSDADSAISSPISYLAQRRPNLSSSHSQQQLAQSVSSVSTDLRPPTGSSSRSSLSRNSIDHNLQLVSPGLNSPWPVPPSSAHTHASSPSGWPKKSATYYPLRTWSNAPRPHPSILASARSPSSALAYLDLSPSPMPLGTDHSTIILPNPSLETSSSPSASDDVSFIPNKGTTLVGFDPTRTRIRPASLISHRSTASRETFYEDAPDKAEWMEIDSGDAYDDLEKTEDTVRKISQRLSLMNGIDAVSHAAKAKSRYHARSENGKSQDSAISIHPFSGPRSSYSPSKQLHRCTSPQSTEHTLISSNRDSSSSSKTTSSSSRSLHDLDPNLSRSSMSMDRASSAGVPLFTRTSFSREDREEERPASRLRRLDDEELAAIPEAPDLESAEPYGFRMTRSPTSMDGGNRRRLKPLILRSTTSNTSSARTRTSLLDPPGRNSQSSGSFSPPQPTYEANSLLTAHGDPWGSSQKSSDDVTLKDSDVLTDKQTVTEPQGLSLPRRIEIQSASTDVDLNDHKQSTNASQKRVELMTPISREAGDPAASPEIKLLNQVEMGSKLSLMVQKQPSSNTMNKESMVTLNIEDSAPIPSELHKSLPDAEAAEDSFARKNSLKSLNPINTQMLTSGRRRVTSHHSVQTGTCEDITLVNSPAKLDSSPTFRSDHPISYRKRCISNLCMPKPSPRSMDRGISAASYSAAKLAERKHSHSSVSSGPKTPDTLIITGPQVPSQQPLIAGPSPEPGSPYQAKEFPGLTEAKLTPPSRKSRLFLSLTRMQARDAKLLDLTTIRNHNVHSSEDCSALNQAQEQQARLIKGKLKRISRPTPLHGEDERLALWGMISNAKISPNSHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3NY15</accession>
    <name>E3NY15_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20420</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS990009">
        <property type="protein sequence ID" value="EFP94464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338883.1">
        <property type="nucleotide sequence ID" value="XM_003338835.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NY15"/>
    <dbReference type="GeneID" id="10527758"/>
    <dbReference type="KEGG" id="pgr:PGTG_20420"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20420"/>
    <dbReference type="HOGENOM" id="CLU_1986955_0_0_1"/>
    <dbReference type="InParanoid" id="E3NY15"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP94464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="134" mass="14136" checksum="61717D7F46A07C3C" modified="2011-01-11" version="1" fragment="single">MSTRSNNPNLHGPLPPATRPRRSKSAGGTGEPFKVDNDSKEDGSNRYEGDGDLGTQRLSPDRRSRYDHGEKNCSPSNESSDLGSGRHGLGSLGAKDSSPDNKSSDHSSGSDGRGSRSFNLFGGYPATPQITGET</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3NY77</accession>
    <name>E3NY77_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20482</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS990048">
        <property type="protein sequence ID" value="EFP94526.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338945.2">
        <property type="nucleotide sequence ID" value="XM_003338897.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NY77"/>
    <dbReference type="GeneID" id="10527905"/>
    <dbReference type="KEGG" id="pgr:PGTG_20482"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20482"/>
    <dbReference type="HOGENOM" id="CLU_2278822_0_0_1"/>
    <dbReference type="InParanoid" id="E3NY77"/>
    <dbReference type="OrthoDB" id="10279497at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP94526.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="102" mass="11398" checksum="4F1CC1BBA84AC13F" modified="2012-04-18" version="2">MVKGWRTDRPCCPISFTQQWKVLLGEQNLVDNVGGKGATHQQSNGPANGAPTQPFSQPGRMGWTTVHKRSYNMAVQPLWSTPSSNQWVVSRLEDRCTSRMVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3NY93</accession>
    <name>E3NY93_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">JmjC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_20498</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS990058">
        <property type="protein sequence ID" value="EFP94542.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338958.2">
        <property type="nucleotide sequence ID" value="XM_003338910.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NY93"/>
    <dbReference type="STRING" id="418459.E3NY93"/>
    <dbReference type="GeneID" id="10527918"/>
    <dbReference type="KEGG" id="pgr:PGTG_20498"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20498"/>
    <dbReference type="HOGENOM" id="CLU_016785_6_0_1"/>
    <dbReference type="InParanoid" id="E3NY93"/>
    <dbReference type="OrthoDB" id="424465at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000305">
        <property type="entry name" value="Clavaminate synthase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041667">
        <property type="entry name" value="Cupin_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461:SF99">
        <property type="entry name" value="BIFUNCTIONAL PEPTIDASE AND (3S)-LYSYL HYDROXYLASE JMJD7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12461">
        <property type="entry name" value="HYPOXIA-INDUCIBLE FACTOR 1 ALPHA INHIBITOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13621">
        <property type="entry name" value="Cupin_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjC" evidence="2">
        <location>
            <begin position="162"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="58"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP94542.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="382" mass="42970" checksum="8F6AB18ACBA7E780" modified="2012-04-18" version="2">MDPSEESLQKLVSEYQELNGSAIDQVSGRLPTALELSRSIARNRPLVIRSYAGLQRASSSSSSSSSSIEEDHHHHHLEGLEEWTDPKLVQRLADQPITVARTPFGNADSIVDGTYFVEPAYEKLTMADFLAELRGCSGTKSSQDGSTDAANQDRASSGSRGDVVYLQSQDGNLSKELRPLLPNVGTHVPIASQALGTNQPDAVNLWIGDDRSITSLHNDPYENFYLMIEGSKTFTLFAPVEYYCMHEGRYRSSQYHWSESDQKWTIIPKSSEEQIVPWIPIDPLEPDYNTYPRFRFARSMSVTLHQGDLLYLPSLWFHHVQAHRTTPNGLIIACNWWYDMNYEGAHYALFSYLRRQVLALDRSGLPNPIPKDVEFGDGSISP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPC1</accession>
    <name>H6QPC1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_20654</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EHS63556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890617.1">
        <property type="nucleotide sequence ID" value="XM_003890568.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPC1"/>
    <dbReference type="GeneID" id="13541503"/>
    <dbReference type="KEGG" id="pgr:PGTG_20654"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20654"/>
    <dbReference type="HOGENOM" id="CLU_1390850_0_0_1"/>
    <dbReference type="InParanoid" id="H6QPC1"/>
    <dbReference type="OrthoDB" id="9984778at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45969:SF71">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_5, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45969">
        <property type="entry name" value="RING ZINC FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003605697" description="RING-type domain-containing protein" evidence="5">
        <location>
            <begin position="23"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="61"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS63556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="196" mass="22365" checksum="9462888835C7680A" modified="2012-04-18" version="1">MLLVPRIMLLQVMVLVTPTINATEQTDLSNSVGPKAPLKSHHQHMKRMELAEHNQYSSELCTICREQLGANSREWPICKHLYHFNCIDEWRKGEDEENNQCPNCRSTDTTLAAQKEAQFRLRRDDRRGLSRVYVASTSSNQSRPPSLSDLSCRWCGGSMRRARVPPASGQWTFRVKLVCQRCNSPVMVHPSALSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPJ2</accession>
    <name>H6QPJ2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_20859</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EHS63877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890572.1">
        <property type="nucleotide sequence ID" value="XM_003890523.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPJ2"/>
    <dbReference type="STRING" id="418459.H6QPJ2"/>
    <dbReference type="GeneID" id="13543230"/>
    <dbReference type="KEGG" id="pgr:PGTG_20859"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20859"/>
    <dbReference type="eggNOG" id="ENOG502QR5Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012390_4_3_1"/>
    <dbReference type="InParanoid" id="H6QPJ2"/>
    <dbReference type="OrthoDB" id="2287304at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47150:SF5">
        <property type="entry name" value="OS07G0546750 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47150">
        <property type="entry name" value="OS12G0169200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="158" mass="18208" checksum="37C4F5478985A964" modified="2012-04-18" version="1">MLIQMRRAREDEELELRVHGGSRPGRMPNLPRDFEAGYNQLFTDYFAENPVYPDHLFRRRFRMRHSLFLSIVDDVQEHDNYFLQKADALGKPGLRPLQKITSAIRMLAYGGASDLNDEYLRLGESTSNESLLKFCQAVIHVYGVWSTGQVRQSLAAVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPP4</accession>
    <name>H6QPP4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Aquaporin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_20798</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>H2O(in) = H2O(out)</text>
            <dbReference type="Rhea" id="RHEA:29667"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EHS64096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890505.1">
        <property type="nucleotide sequence ID" value="XM_003890456.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPP4"/>
    <dbReference type="FunCoup" id="H6QPP4">
        <property type="interactions" value="37"/>
    </dbReference>
    <dbReference type="STRING" id="418459.H6QPP4"/>
    <dbReference type="GeneID" id="13542950"/>
    <dbReference type="KEGG" id="pgr:PGTG_20798"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20798"/>
    <dbReference type="eggNOG" id="KOG0224">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_484083_0_0_1"/>
    <dbReference type="InParanoid" id="H6QPP4"/>
    <dbReference type="OrthoDB" id="3222at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015254">
        <property type="term" value="F:glycerol channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015793">
        <property type="term" value="P:glycerol transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006833">
        <property type="term" value="P:water transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00333">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000027">
        <property type="entry name" value="MIP aquaporin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050363">
        <property type="entry name" value="MIP/Aquaporin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022357">
        <property type="entry name" value="MIP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43829">
        <property type="entry name" value="AQUAPORIN OR AQUAGLYCEROPORIN RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43829:SF9">
        <property type="entry name" value="AQUAPORIN-9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00221">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="290"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="326"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="433"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="462"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="520"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="69"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="177"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="226"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS64096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="586" mass="64818" checksum="ABE848A1E311D6F5" modified="2012-04-18" version="1">MQAENEAAETIDSATWNQPQPDPAAEPETRGTSLDNDRGRGNYVSSNMTHEATIGRPAHRLMRLQTFSLSELNPQKSPERDRPLYSLGRPFPEPTALRRRSPTKLIEDIAAQMNLQTGSLKNFPISTNRPPESRAELAAGSVTQTGMHILRLGDLSEDQYIVATPELLRSFLEETRNRDSRRVRTSFPVDDHRNSRGTQASQRRHRADEYQDPVARPEFIGRPDLSESTAVEQNEKDNVPVSPKSYRSSNVSFVRGAGGLPPVTIARDHTGNPTYSSRYRRFKSLIREPAAEALASFLLAVFSGGASTQVYLTANSAISSSPKGNYATQCVGSAIAVMIGVYVAGGISGGHLNPTVTISLAIFRDFPWQKVIPYCSAQVFGGLMGSLTIQLIYAGGLDVYEGGEGIRTISGPSSTAGFFLSYPAPYMSNFCSFFQQFLDSTIVLLFIMAIGDRTNSPPPDGISPIVFMCVVIGVAFALGSQTSYALNPALDLSSRIVVTLMGYGTSHWSFRDQYWIWNNWVSNLLGALFGCFLYDFFIYEGDDSPLNRPWSWHSPLNLSPRSWRVSFPSIRRRKTSQEKPAEIFKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPW3</accession>
    <name>H6QPW3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20914</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EHS64301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890457.1">
        <property type="nucleotide sequence ID" value="XM_003890408.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPW3"/>
    <dbReference type="GeneID" id="13542602"/>
    <dbReference type="KEGG" id="pgr:PGTG_20914"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20914"/>
    <dbReference type="HOGENOM" id="CLU_071366_0_0_1"/>
    <dbReference type="InParanoid" id="H6QPW3"/>
    <dbReference type="OrthoDB" id="2504017at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003606510" evidence="1">
        <location>
            <begin position="20"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="358" mass="41160" checksum="6FA50257AD605199" modified="2012-04-18" version="1">MRFQFILVCLITIGCGMDARPPFQLEPAEVQTGLVGTRRDKTIINPSWEDDLEERHPLVVFSDKVPQRTDNSQDPYQASLFEKLIKTSPLLKSLSSRERSTIIKNWEGNKENVISLCVASKKLWELHLKMEKLCAWFETITSRDVFSSAKKLLEQSKKHIFVIKQARLLELGQVDSYSNLRLIEEPVGLFNQQGTVNDLPGQQSKSLDSFHPDILRMIAGKFWQEYGKSMWREMKALSIKCASQFQGFLTSSDPAFLWNVPARIYLFQTLNLLFKNGFINREAVRGSFHDPVLIKEVIYYAISGFKDEYKSKDDFLGDYLSITKHPYWPLLNESFNGNLILVSLLSSSTFKISMYSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQ06</accession>
    <name>H6QQ06_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20952</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EHS64489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890403.1">
        <property type="nucleotide sequence ID" value="XM_003890354.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQ06"/>
    <dbReference type="GeneID" id="13542676"/>
    <dbReference type="KEGG" id="pgr:PGTG_20952"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20952"/>
    <dbReference type="HOGENOM" id="CLU_2759008_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQ06"/>
    <dbReference type="OrthoDB" id="10282796at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="74" mass="8707" checksum="FED4B9A8EE675E17" modified="2012-04-18" version="1">MPQKRGAEDQEPINVDSGSDIESVKAPFKAKSTRTSKKSWVWHPRLYPKSNGRRDPRRTYLPERLACRRHDQCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQD7</accession>
    <name>H6QQD7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EHS62536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890249.1">
        <property type="nucleotide sequence ID" value="XM_003890200.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQD7"/>
    <dbReference type="GeneID" id="13541204"/>
    <dbReference type="KEGG" id="pgr:PGTG_21085"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21085"/>
    <dbReference type="HOGENOM" id="CLU_2265032_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQD7"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="103" mass="9927" checksum="BD3AFFDF0A229F9F" modified="2012-04-18" version="1">MGSMPSLPGMSSLPSLPSVGSMPSLPGMSSLPSLPGTSSLPSIPSMSSLPSLPGMSSLPSLPGMSSSSSQSSLPSLPSMPSVPGINSLTSGGLPGLSSLTSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRR7</accession>
    <name>H6QRR7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_21539</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EHS63364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889802.1">
        <property type="nucleotide sequence ID" value="XM_003889753.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRR7"/>
    <dbReference type="GeneID" id="13543049"/>
    <dbReference type="KEGG" id="pgr:PGTG_21539"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21539"/>
    <dbReference type="eggNOG" id="ENOG502SCJN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032241_1_0_1"/>
    <dbReference type="InParanoid" id="H6QRR7"/>
    <dbReference type="OrthoDB" id="2507256at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="215"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="407"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="16"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="63"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="77"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="215"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="313"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="348"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS63364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="455" mass="50950" checksum="8779D6B935088279" modified="2012-04-18" version="1">MQSFEEVDPSLLAASQDPTFSVPSLNSIASKPPPNKKHTRRTKKQMALARGEDITNNEQTASQKRHKSSQRQRGSQKKGTQSTQKTITENDNNPQFILTDYENICSYLEDDEKFSELYGDTKTNVGPKVLTKTAAFNIFAIFINAHSNKRLNLTGRQLQQRVNYYIRKKYFPAKQWENQTGAGILEMDPHASIDEALEAKCPCYAKMDAIFAEFDSSNPTPTGPSQKNTQERLAEFDSSNPTPTGPRENNTQDRESSPEVFYPGWDESESPPAAQRSVGDNVVESMIPPLLGDGEESDVLPQSTPASVPASLVRTNPDCTQGQAQSTPTPAAPRRGFPNQANSNETGPPRDRADKSKSSLATAYQSVSDKKYEMLKSALKDKKTNDDRRFEWDKERYEKDQSETKLQRESEEKMQAARLEAEEKKQNARLAAAERWISQGKSVTEIDILLKAVFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>H6QS32</accession>
    <name>H6QS32_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21607</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EHS63478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889689.1">
        <property type="nucleotide sequence ID" value="XM_003889640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QS32"/>
    <dbReference type="GeneID" id="13542829"/>
    <dbReference type="KEGG" id="pgr:PGTG_21607"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21607"/>
    <dbReference type="HOGENOM" id="CLU_2886851_0_0_1"/>
    <dbReference type="InParanoid" id="H6QS32"/>
    <dbReference type="OrthoDB" id="2499534at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="63" mass="7343" checksum="2640721FA06DB1CE" modified="2012-04-18" version="1">MGNFKYNNDGICSYAPIYLFQHPDYCNFNLFLLAFIMVSGYGLLLLGNRLDAEKEKEKESKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>H6QS84</accession>
    <name>H6QS84_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_21651</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178290">
        <property type="protein sequence ID" value="EHS63548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889637.1">
        <property type="nucleotide sequence ID" value="XM_003889588.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QS84"/>
    <dbReference type="STRING" id="418459.H6QS84"/>
    <dbReference type="GeneID" id="13542893"/>
    <dbReference type="KEGG" id="pgr:PGTG_21651"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21651"/>
    <dbReference type="HOGENOM" id="CLU_012390_7_2_1"/>
    <dbReference type="InParanoid" id="H6QS84"/>
    <dbReference type="OrthoDB" id="2507429at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125">
        <property type="entry name" value="F21J9.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125:SF3">
        <property type="entry name" value="NO-APICAL-MERISTEM-ASSOCIATED CARBOXY-TERMINAL DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="2">
        <location>
            <begin position="202"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="240"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS63548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="376" mass="41499" checksum="293E66DE937EED58" modified="2012-04-18" version="1">MSTTDDTPSSTASTPKNPPKKTVKPKKLVQNKKKETPKANATPKANATPKAVQTPNVIDTPNAAETPKAIDPVPNQATQSEAGDDSDSEDEEGEKKSRAPNYQEHEDLQLCTSWLETTEDGRKGTDQTGKAFWGTVTSHYHSKITDPKRSIRSIKGRWGLIQHALNKFHGCVNQINHRNPSGTTATNRISMALTLFTQLHDKPFTYMCCYNLLSTSPKWHEYNISMDKKHEGRKATNVNPSSPLPGFLPSSTPADTTRGTSEASEDESTQQEPTRPIGRKKAKVAYQEEKLDASNHEHLKKMASAHVDIAEAVKNQKNAISAQQSALERLADEAIMSKDLTGADDDVRRYYSLQRKRILARLEKEVSESAPAAPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>H6QU34</accession>
    <name>H6QU34_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_22260</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178328">
        <property type="protein sequence ID" value="EHS64446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888973.1">
        <property type="nucleotide sequence ID" value="XM_003888924.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QU34"/>
    <dbReference type="GeneID" id="13541052"/>
    <dbReference type="KEGG" id="pgr:PGTG_22260"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22260"/>
    <dbReference type="HOGENOM" id="CLU_003292_0_1_1"/>
    <dbReference type="InParanoid" id="H6QU34"/>
    <dbReference type="OrthoDB" id="5149081at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052055">
        <property type="entry name" value="Hepadnavirus_pol/RT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050:SF7">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47823">
        <property type="entry name" value="lambda integrase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS64446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="820" mass="92428" checksum="5F2E3EF4B38AB584" modified="2012-04-18" version="1">MNVDKWRDALGANHLLPEYQDVIDGFTHGFDQGIPQHTIEKKRWFTPDNHKSSLLAKDKIQESIAKELAAGRMKGPFSHHQMEATFGFFRSNPLGAVVNGDGKIRPINDLSYPRNDTDVKSVNSYVNKLDFETTWDDFKTVSKFFAEDRRKFELALFDWEGAYRQIPTRRDQWKYLLVQDFDGNLLIDTRITFGGVAGCGSFGHPADAWKLIMKNHFDLVTVFRWVDDNLFVKEIGVDLPMKDIVLKSTELGVMTNIKKFSDFSTEQKFIGFVWDGVNKTVRLPEGKIEKRLSQIYPFQVPKASFDYEDAEVPSVLFISLVKIVDLEESKRPTPVDVLEDLQIWVETLGNFEHTRLIRWGPPLDVGWVGDASTSFGIGILIGKHWAQFKLVDPHSNKKRISLLETIAIRLGLIMLLKLRDQRGKSLIVWTDNTTTENSINNMKTKDKDANDEWKKIQKILLTESVNLIARRVTSKDNKADALSRGLRSGQPVKHQVVIQVPSDLQDLSTLKSYNSAVRKFLAFKKAQGLPTFLLPATEDDIYEFCYWAGKTDVDGGESGVLAVTLKKYLQGLKAWHIFHDSPYPVISERKAALLLKSSLKVETLTPKKEPKKPVLIKHLVTLANDLSSGSLEDSAILDLALVAFWSLARLGELTTDQATPRRKILKSHVSIDSAGTSATIELKQSKTALPGESQFLQLKGMPNILCPVRALTRRLATCGQSNHLFGYRNGDSHVSLTKYFVRKRLAAVWAAHGFTGLSGHSFRVGGASFHNAVGIAPAQICKVGRWASASYKLYIRDYSENDLLISLQLLARLDLQWRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUW7</accession>
    <name>H6QUW7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_22538</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178360">
        <property type="protein sequence ID" value="EHS64875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888701.1">
        <property type="nucleotide sequence ID" value="XM_003888652.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUW7"/>
    <dbReference type="GeneID" id="13542174"/>
    <dbReference type="KEGG" id="pgr:PGTG_22538"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22538"/>
    <dbReference type="eggNOG" id="ENOG502SMER">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011407_0_0_1"/>
    <dbReference type="InParanoid" id="H6QUW7"/>
    <dbReference type="OrthoDB" id="2500537at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041320">
        <property type="entry name" value="CxC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040521">
        <property type="entry name" value="KDZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18802">
        <property type="entry name" value="CxC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18758">
        <property type="entry name" value="KDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxC1-like cysteine cluster associated with KDZ transposases" evidence="2">
        <location>
            <begin position="160"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS64875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="442" mass="50701" checksum="F6700F66A0693757" modified="2012-04-18" version="1">MPLFEGDGGKRRKRIRKPKTEAGVSFSQAVQLEHQHILQTPLAQPANDIHPTDHPNEDSSAQENNNQTFDPTGQDDFDFGADHIGNNGLDPFQSAHQQDWLPSDHQDNDHLTNSRVQQLHRYFQSEHYKQKKILEEKNWEEAYQEMFSSFQQCAIKTFEWGNPNLWNHDWNEPCNCNNLRSRPVVLVDMYRRTQTEVSFCKCQSDQVRLIRMGYIGGSPKFPRTAFSIRLLRFHHIIWKHCAVSITPFSKALDEFLDSNNPLILVSPPTLQSESCYTTRQWRQTISAAIDAYREMIRRGQIILEELLQMGPMDKLANICPKCYGPPVPGKTPDEPDYIVCMDGNFQHRRHLAASQELHQSRKPNTLFISPAEVQEMELSMVSSRNTLAADETLDQCTEQHTAANDTRGAATWKACDDTGIFGLACRHDQILRLINIVQSGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>H6QVM0</accession>
    <name>H6QVM0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22791</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS989963">
        <property type="protein sequence ID" value="EHS63342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890880.1">
        <property type="nucleotide sequence ID" value="XM_003890831.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QVM0"/>
    <dbReference type="STRING" id="418459.H6QVM0"/>
    <dbReference type="GeneID" id="13541065"/>
    <dbReference type="KEGG" id="pgr:PGTG_22791"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22791"/>
    <dbReference type="HOGENOM" id="CLU_983984_0_0_1"/>
    <dbReference type="InParanoid" id="H6QVM0"/>
    <dbReference type="OrthoDB" id="2506242at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="249" mass="27705" checksum="88B11B2772FB6B30" modified="2012-04-18" version="1">MARLLPSLPSTNPSPGTDHSYPGRFSPPNTSSNGGINIDEAIARHLRPPPYHNLPRPPHLPFPPAVYSPMHGGRANRVFHPNGPAIGVPFGYGHGPVNPDQFLAGLVPQAQPAAQSHPASPNLPANVAREPSNTALVASSESFNTAEDFLTPQIRVASPTPAGMYDPDDYHHLARHQVESLLDRRAAPERFDRYEFTPQHFDDFYQSATTALVHHFTRHPAANANERQDRVRLFRFITRRYRTMTRRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>H6QVM6</accession>
    <name>H6QVM6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_22797</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS989973">
        <property type="protein sequence ID" value="EHS63382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890873.1">
        <property type="nucleotide sequence ID" value="XM_003890824.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QVM6"/>
    <dbReference type="STRING" id="418459.H6QVM6"/>
    <dbReference type="GeneID" id="13541073"/>
    <dbReference type="KEGG" id="pgr:PGTG_22797"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22797"/>
    <dbReference type="HOGENOM" id="CLU_111672_0_0_1"/>
    <dbReference type="InParanoid" id="H6QVM6"/>
    <dbReference type="OrthoDB" id="2505995at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="2">
        <location>
            <begin position="2"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS63382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="152" mass="16889" checksum="CB984B9BC3484609" modified="2012-04-18" version="1">MFNLDHCWGILKDTPKWLATKQENDLKAKKSKEPKEPSLTPATDGTNPSSPPPDGPGKEDDGYNHGVLGDDSRPDGNKAAKRKRNEDLMLENVLKMQEEMVKVSQEQSIAVKAGMKLAANDHIMSTDLTGMGNKTKAYWKKKRRAILDCPDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQ18</accession>
    <name>E3JQ18_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S ribosomal protein L36</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_00057</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eL36 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307107.1">
        <property type="nucleotide sequence ID" value="XM_003307059.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQ18"/>
    <dbReference type="FunCoup" id="E3JQ18">
        <property type="interactions" value="322"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JQ18"/>
    <dbReference type="GeneID" id="10527378"/>
    <dbReference type="KEGG" id="pgr:PGTG_00057"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00057"/>
    <dbReference type="eggNOG" id="KOG3452">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_140672_0_0_1"/>
    <dbReference type="InParanoid" id="E3JQ18"/>
    <dbReference type="OMA" id="NKGHKTE"/>
    <dbReference type="OrthoDB" id="9616667at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1760:FF:000003">
        <property type="entry name" value="60S ribosomal protein L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1760">
        <property type="entry name" value="60S ribosomal protein L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000509">
        <property type="entry name" value="Ribosomal_eL36"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038097">
        <property type="entry name" value="Ribosomal_eL36_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10114">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01158">
        <property type="entry name" value="Ribosomal_L36e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01190">
        <property type="entry name" value="RIBOSOMAL_L36E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP74101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="111" mass="12573" checksum="E4B46F7D129BD7A8" modified="2011-01-11" version="1">MARAQSTRVAATRTGLPRGLNSGHKTTPRVLPNKPSHFKGRASKRTTFVRSIVREVAGFAPYERRVMELIRNSKDKKARKLTKRRLGTLRRAKRKVDELTGVIAETRRAGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQ40</accession>
    <name>E3JQ40_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00079</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307129.1">
        <property type="nucleotide sequence ID" value="XM_003307081.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQ40"/>
    <dbReference type="GeneID" id="10527860"/>
    <dbReference type="KEGG" id="pgr:PGTG_00079"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00079"/>
    <dbReference type="HOGENOM" id="CLU_522886_0_0_1"/>
    <dbReference type="InParanoid" id="E3JQ40"/>
    <dbReference type="OrthoDB" id="2505496at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="188"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="234"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="348"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="95"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="188"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="253"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="283"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="348"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="382"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="491"/>
            <end position="503"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP74123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="521" mass="57727" checksum="4A8CC1B233A77EF0" modified="2011-01-11" version="1">MDTFPSRTTTNTTTAMTATTHHTNNTTTTTTLPTSIATITTNQNTLINRNQEEEEEEEEGQGQEQDEEDEEEQNYISDPLQKWLVFDPSSSPPIQEEEEEEELDQDHLSSSTSSFQSINHSSIYHDTSMPSHDGRGHFFSPPDHSLSPDGSEFEYHSIPHNLPLPITTQSLSSLSDLDSQEDDLLSIPSSLSITRSGSSRSSNLRTHTHQEMSPEDAELLLTPMAMSAGFSHHSTIVGSTHEPGRVSSRSSHHSTDQQRARRSEPTSHSRRTHSRSSRRSRITHISSSHRSSSRTKPPIVIIPSSSSASSDNKNINHLSQHRIAAISFLTSVASKLLDLDSDTMNMLSTPPENQTIKNSTNHLSSSASTTPTKTRPPIRFSIGDDSRTSLTHEQDMISSPLDLSGFMPPSSKSVWRTTEEREEEEEQDGTQNDGKEGSGRMKSGQYGATSTLKIPHSRSVDRLRSSPSSTITPHLHRRSSSTLLLQDSRSRSLPPNNSEIPSTDSMASVLLNKVLNEWTNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQY1</accession>
    <name>E3JQY1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">3-carboxymuconate cyclase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_00194</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cycloisomerase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307244.1">
        <property type="nucleotide sequence ID" value="XM_003307196.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQY1"/>
    <dbReference type="GeneID" id="10527534"/>
    <dbReference type="KEGG" id="pgr:PGTG_00194"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00194"/>
    <dbReference type="HOGENOM" id="CLU_038716_0_1_1"/>
    <dbReference type="InParanoid" id="E3JQY1"/>
    <dbReference type="OrthoDB" id="2508186at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017057">
        <property type="term" value="F:6-phosphogluconolactonase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050282">
        <property type="entry name" value="Cycloisomerase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019405">
        <property type="entry name" value="Lactonase_7-beta_prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30344:SF1">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30344">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10282">
        <property type="entry name" value="Lactonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172073" description="3-carboxymuconate cyclase" evidence="2">
        <location>
            <begin position="27"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP74238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="402" mass="42845" checksum="A8970A096E5A599F" modified="2011-01-11" version="1">MNPLSNNLNRLFSVACLASWSGMLTAEPTLTDTLPASLKVYIGGQDKVVNVFIYDTTKNTFTPKTSSDALGANTTWFQVDKTNQFIISASSGHPPDQAGIFAASVSADGTLKRTTSIPLNGNPHVSAAFDRDDTTVTITTGNVNAESDLNSYKLILDGTKKPEPEATVRLQAFPAPTDPKVFAYASQVKRNPSGNIYLIPFSNLDSIGSQTIAPFTSAQNSDIVVKSGCGPQSLTFPPGDVSTETNMIFYLLCQRSKEILLIEVPNPTGFPLAPILHNSISTLASGAKAEDFDATETLITPDGKFLYTISVPKVSNGKQENMFTVFARDAATGNLVQTGTFKTGGKGSRNFRFSPDKAASLIIVSNTDSNLVCVHKRDPQTGALTQVGSTKLNSPGFPLFVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRH5</accession>
    <name>E3JRH5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_00404</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74448.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307454.2">
        <property type="nucleotide sequence ID" value="XM_003307406.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JRH5"/>
    <dbReference type="GeneID" id="10528090"/>
    <dbReference type="KEGG" id="pgr:PGTG_00404"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00404"/>
    <dbReference type="HOGENOM" id="CLU_121075_0_0_1"/>
    <dbReference type="InParanoid" id="E3JRH5"/>
    <dbReference type="OrthoDB" id="10284997at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP74448.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="108" mass="12190" checksum="FF1469323978E573" modified="2012-04-18" version="2">MVTFLSMTPSHAFICDNAFDLHGPTTRGGEERAMPYLVFIRCVDKANPSQSISVAPVNYHIHNDEQLDVWGYEVYRDTNIKASKLGAYLCPRKNPWRPECDDDACVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRH6</accession>
    <name>E3JRH6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF1279 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_00405</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74449.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307455.2">
        <property type="nucleotide sequence ID" value="XM_003307407.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JRH6"/>
    <dbReference type="FunCoup" id="E3JRH6">
        <property type="interactions" value="157"/>
    </dbReference>
    <dbReference type="GeneID" id="10528034"/>
    <dbReference type="KEGG" id="pgr:PGTG_00405"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00405"/>
    <dbReference type="eggNOG" id="KOG4526">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_059211_1_0_1"/>
    <dbReference type="InParanoid" id="E3JRH6"/>
    <dbReference type="OrthoDB" id="426386at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045866">
        <property type="entry name" value="FAM210A/B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009688">
        <property type="entry name" value="FAM210A/B-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21377">
        <property type="entry name" value="PROTEIN FAM210B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21377:SF0">
        <property type="entry name" value="PROTEIN FAM210B, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06916">
        <property type="entry name" value="FAM210A-B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="150"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="DUF1279" evidence="3">
        <location>
            <begin position="143"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="200"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP74449.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="275" mass="30821" checksum="4DF4672198EEFC3A" modified="2012-04-18" version="2">MIRTGRIEWIVSKPLVRLYSNGISSSGAGLTKTWETRKGRRLLDRTEFPHSNHRSLRYLPFSKISSSPITAPSPSSSSASSSSSNPTPHSIDHYPKTTSSPYFSLRSFFSKLTDHHQQQEQQDQKSSEFRGEERADPVGLFGKLRQLTKLYGSAALVVYGLLGGIDFGLSFVTIYLVGAEHVRKAEDWLLDQLNWKRAHSTSPLPSPTATTNTTTDGEEATKRDDNNMLWTTAVVAYTIHKTILLPFRIGLTAWITPPIVRHLRKRGWKVGRNLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3JST7</accession>
    <name>E3JST7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01705</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75112.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319531.2">
        <property type="nucleotide sequence ID" value="XM_003319483.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JST7"/>
    <dbReference type="GeneID" id="10547554"/>
    <dbReference type="KEGG" id="pgr:PGTG_01705"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01705"/>
    <dbReference type="HOGENOM" id="CLU_146865_0_0_1"/>
    <dbReference type="InParanoid" id="E3JST7"/>
    <dbReference type="OrthoDB" id="2500274at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172920" evidence="1">
        <location>
            <begin position="21"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75112.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="149" mass="16885" checksum="BCC4621347795C0C" modified="2012-04-18" version="2">MLFLGSNFLLLSLPLEPSVAQPISVNSIIQRNLYRHAKVELLFLGSNPTPLEPSVAQPIAVKSIIQRNLYRHAKVELGIYLLLDSEPIDSNDSLSSLSSSGHQSSSSTEDYWNDCSETSISFDEFIPAHLYTPRRLPIAQLIKNWWRYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>E3JTH9</accession>
    <name>E3JTH9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ATP-dependent RNA helicase</fullName>
            <ecNumber evidence="10">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGTG_01897</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">RNA helicase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="10">The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="10"/>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319723.1">
        <property type="nucleotide sequence ID" value="XM_003319675.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JTH9"/>
    <dbReference type="FunCoup" id="E3JTH9">
        <property type="interactions" value="858"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JTH9"/>
    <dbReference type="GeneID" id="10542770"/>
    <dbReference type="KEGG" id="pgr:PGTG_01897"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01897"/>
    <dbReference type="eggNOG" id="KOG0343">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_26_1_1"/>
    <dbReference type="InParanoid" id="E3JTH9"/>
    <dbReference type="OMA" id="YDKMFER"/>
    <dbReference type="OrthoDB" id="10259640at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17941">
        <property type="entry name" value="DEADc_DDX10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025313">
        <property type="entry name" value="SPB4-like_CTE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24031">
        <property type="entry name" value="RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13959">
        <property type="entry name" value="CTE_SPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01178">
        <property type="entry name" value="DUF4217"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 10" id="KW-0347">Helicase</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="8 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="14">
        <location>
            <begin position="45"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="76"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="13">
        <location>
            <begin position="280"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="496"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="729"/>
            <end position="832"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="9">
        <location>
            <begin position="45"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="11">
        <location>
            <begin position="496"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="524"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="558"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="583"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="729"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="747"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="809"/>
            <end position="818"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EFP75304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="844" mass="94127" checksum="D9448723DF815798" modified="2011-01-11" version="1">MKASSQKKKSKQPIKPRKLKRQTEEEEIKKLASESISFDLAKPPTLFSDLPISRATLKGLQAAKFEKLTPIQALAIPRALKGRDVLGAARTGSGKTLAFLIPVLEMLYRQKWGAMDGLGALIISPTRELAVQIFEVLRSIGKFHAFSAGLVIGGKSLEDERDRLGRMNILVSTPGRLQQHLEQTTNFDSDNLQVLVLDEADRILDMGFANSVNAIISSLPNSRQSLLFSATQTKSVKDLARLSLTGDPEYVSARETGVERDLTTPKELVQSYMVTPLECKIDYLWGFLKTHLKTKMIVFLSSCKQVRFVHEIFRHLRPGIPLLHLHGKQKQVKRLEIYERFSSSPQVCLFATDIAARGLDFPSVDWVVQVDCPEDVDTYIHRVGRTARYQSGGKALLLLLPSEEEGMSKKWEARGIVVSKVKPNESKKQSIQNQIQAQMFKFPELKFLGQRAFISYVRSIHLQKNKEIFKLKELDLVKFAESMGLPGAPQVRFSGSGAGGAAGGTGESAKAKKNAPRDLETLKKLTLTQNTTSTDAVNKGDADENEEASDSNATSSEDGSDSDSDSDDDSDENDSNDGEEQTHSVTIAPHSSATHPTGVKPTVRTKYDRMFQRQNQDILSQHYAKLVDRSEDAAEGREKGDEEEDDFIQLKRADHDLDAAEAAIPDSAYLSKRKLKQATSKKAVAMSGIGLGKKVIFDDEGEAHQAYKILQEEEYKKNHDVGSEIKDFVTREKDKVQQADQTDKLVAKNKRAEKKRKRKLTSEANQNEGVQGARVAELASNDEDDGYVSPEFDLPPESDSDPDGDRDHHQHRPKKLKAFRPSDRDDDEEEDLEALALQKLQQRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3JTU7</accession>
    <name>E3JTU7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">PH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_00760</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the OSBP family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319848.1">
        <property type="nucleotide sequence ID" value="XM_003319800.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JTU7"/>
    <dbReference type="STRING" id="418459.E3JTU7"/>
    <dbReference type="GeneID" id="10543056"/>
    <dbReference type="KEGG" id="pgr:PGTG_00760"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00760"/>
    <dbReference type="eggNOG" id="KOG1737">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_771923_0_0_1"/>
    <dbReference type="InParanoid" id="E3JTU7"/>
    <dbReference type="OrthoDB" id="416222at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035621">
        <property type="term" value="P:ER to Golgi ceramide transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030011">
        <property type="term" value="P:maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13289">
        <property type="entry name" value="PH_Osh3p_yeast"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041680">
        <property type="entry name" value="PH_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15409">
        <property type="entry name" value="PH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PH" evidence="3">
        <location>
            <begin position="19"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="120"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="185"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="283"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="120"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="196"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="206"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="283"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="308"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="345"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP75429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="359" mass="40186" checksum="E1A1BC4EEDDA5E1C" modified="2011-01-11" version="1">MASSPTELISRSPSTLLGHPILEGWMLKKKRKKMQGFARRYFYLSQEPAVLSYSFSPNSRIRDSVLIKLASISISRKSRSIHIDSGGSLVMHIKTLSDPDFLRWTQTLQSLVTNGKLKECQRKPRQAPRDTSKALPRYSEFNRSDAGPSVRPVELSNLYHSVARMQAPLSMLQEVQNDLRELLFPPQSEPLSAPQPSTPGQPRSPPTMSQPAYNPTTSPKLQYSTPKGKKQHLFKHYSHNHVTSSVDAHKVYQTITQAVTQLKTEHLNLIDLINIHRTSMISDQPSKLSQTNPRTSPSVHITRNPRQSTSSYSHGASASDYVSSSSTVDSQLSSRRRSSTVTKASQRDRVGDGGKPRFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3JU29</accession>
    <name>E3JU29_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_00885</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319973.1">
        <property type="nucleotide sequence ID" value="XM_003319925.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JU29"/>
    <dbReference type="GeneID" id="10543270"/>
    <dbReference type="KEGG" id="pgr:PGTG_00885"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00885"/>
    <dbReference type="HOGENOM" id="CLU_059061_0_0_1"/>
    <dbReference type="InParanoid" id="E3JU29"/>
    <dbReference type="OrthoDB" id="10283741at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP75554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="379" mass="43500" checksum="FB019086520FBE07" modified="2011-01-11" version="1">MQEQEMSQQAHQPPYTPIIYDEIAAQFAQTLESSFSYLREDLDHLIILGAYGGDYMISPESGSHFNEIKTVVLPCLSRSLRQMQDLLTSPSYYQGGKDPDYQTGAKLLKEVEHTTDRLMHLNQSLLLQGQPRYFRGDWDMNAYRCRAMQLSIVDILRKTAEILRRTAQIPCYHRYLLSQSATLTMKKRDMEELSQITYHQTHLMLNSIDNIIRSFEPSDLELLGDRCRQVVQKIESEIAHQVKDSIARARMHIPSSQDALTIIKLTRLFLNKLSKATNGIPHPLPLMSSDQLLALIKATDHLDDSLRMFSIPTEQGPDPKKAYNLLTQVLSAIKILTDHSGRQGPSPESPYDLPRKCCEWYQLFSHQLSAVVTRLWLRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>E3JV40</accession>
    <name>E3JV40_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ubiquitin-conjugating enzyme E2 G1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_01246</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75915.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320334.2">
        <property type="nucleotide sequence ID" value="XM_003320286.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JV40"/>
    <dbReference type="STRING" id="418459.E3JV40"/>
    <dbReference type="GeneID" id="10532993"/>
    <dbReference type="KEGG" id="pgr:PGTG_01246"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01246"/>
    <dbReference type="HOGENOM" id="CLU_030988_10_3_1"/>
    <dbReference type="InParanoid" id="E3JV40"/>
    <dbReference type="OrthoDB" id="19692at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="5">
        <location>
            <begin position="1"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="3">
        <location>
            <position position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP75915.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="113" mass="12682" checksum="A117854D508617ED" modified="2012-04-18" version="2">MSFPAKFPLLPPKLRFTTQMWHPNIYPDGTVCISILHPPGNDEYGYEDAGEHWLPVHTIESILVSVTSLLASESPNTESPANIDAAKELRDDPASYKKKVRRLARKSAEDCFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3JVM9</accession>
    <name>E3JVM9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Large subunit ribosomal protein L26e</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_02545</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320523.1">
        <property type="nucleotide sequence ID" value="XM_003320475.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JVM9"/>
    <dbReference type="FunCoup" id="E3JVM9">
        <property type="interactions" value="426"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JVM9"/>
    <dbReference type="GeneID" id="10533508"/>
    <dbReference type="KEGG" id="pgr:PGTG_02545"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02545"/>
    <dbReference type="eggNOG" id="KOG3401">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093240_0_1_1"/>
    <dbReference type="InParanoid" id="E3JVM9"/>
    <dbReference type="OMA" id="VRIMRGD"/>
    <dbReference type="OrthoDB" id="1688503at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000009">
        <property type="entry name" value="60S ribosomal protein L26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01326_A">
        <property type="entry name" value="Ribosomal_uL24_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005825">
        <property type="entry name" value="Ribosomal_uL24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005756">
        <property type="entry name" value="Ribosomal_uL24_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="Ribosomal_uL24_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01080">
        <property type="entry name" value="rplX_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11143">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L26 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16906">
        <property type="entry name" value="Ribosomal_L26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01108">
        <property type="entry name" value="RIBOSOMAL_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="KOW" evidence="5">
        <location>
            <begin position="49"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP76104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="134" mass="15322" checksum="20BF2CBE0452ECB2" modified="2011-01-11" version="1">MAKFSKDVSSSRRKSRKAHFQAPSHERRIIMSSGLSKELRAKYNVRSMPIRKDDEVVVVRGAFKGREGKVLSVYRKKYVIHVDKVTRDKASGQTVQIGVHPSKVVISKLYLDKDRKAILARKDRGSPKEKMQVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>E3JW12</accession>
    <name>E3JW12_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Capsular associated protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_02678</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76237.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320656.2">
        <property type="nucleotide sequence ID" value="XM_003320608.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JW12"/>
    <dbReference type="STRING" id="418459.E3JW12"/>
    <dbReference type="GeneID" id="10533967"/>
    <dbReference type="KEGG" id="pgr:PGTG_02678"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02678"/>
    <dbReference type="eggNOG" id="ENOG502QQ3T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029715_0_0_1"/>
    <dbReference type="InParanoid" id="E3JW12"/>
    <dbReference type="OrthoDB" id="544608at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00229">
        <property type="entry name" value="SGNH_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34407">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34407:SF1">
        <property type="entry name" value="SGNH HYDROLASE-TYPE ESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="395"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP76237.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="595" mass="64258" checksum="77598722A967C675" modified="2012-04-18" version="2">MHKRHPSSSSIPTVNSRTSTSPSRATLISPRSPLHSRTSLLSRRRLLIVLAILSSIFILIHFISWSGSPIGPLPGFATRSAGSRRTDAYLISARRQRLLQGASNSPGGLGPANFSDLAPFTFCPTFGETDELGQVYGSQALLKTRTHVGSSDRIRRVIRRAMAGLPITIGILGGSISSCHGLDATAAHPLGNPIGPNCYPHRIFTWLNDVFPHPANELTNGALRRTGTAYYGFCSAMHLPDRVDLVIVEFDTEDPHEPETISTTDLLIRSLLLRPDQPAVILLGHFAPQFQNEHGFTGPEVWHSAVAHFYDVPHLTVKGLLYEEYLANPEKVSQSYFIDPVLANAKGHELLADTVISFLESEICVVWDEAALEADLAGLSPPVLGDASLMSGSQANTAPSLLGGKGLRKGSEPGAEGSDESSGTLGLSKTAKAGYSKVPAYRIHDKPHTLAGFREIKPSCASANDLINPLPQSVFINSGWSPVAPKLDAEDERHYWFSEKPGSLIKIPLKVSGGDIAVYYQKGPKKDGWGTVKCWVDDNVAGAVEIPGFWDQDHAKPVISQVDRGVGKGSHYVVCELQGKPGDQAKFKIYGIFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWV6</accession>
    <name>E3JWV6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ubiquitin-like protease family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_02972</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C48 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76531.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320950.2">
        <property type="nucleotide sequence ID" value="XM_003320902.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JWV6"/>
    <dbReference type="STRING" id="418459.E3JWV6"/>
    <dbReference type="GeneID" id="10528304"/>
    <dbReference type="KEGG" id="pgr:PGTG_02972"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02972"/>
    <dbReference type="eggNOG" id="KOG0778">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024324_5_2_1"/>
    <dbReference type="InParanoid" id="E3JWV6"/>
    <dbReference type="OrthoDB" id="2496817at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016929">
        <property type="term" value="F:deSUMOylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016926">
        <property type="term" value="P:protein desumoylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.395.10:FF:000024">
        <property type="entry name" value="Chromosome 1, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.395.10">
        <property type="entry name" value="Adenoviral Proteinase, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003653">
        <property type="entry name" value="Peptidase_C48_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606:SF141">
        <property type="entry name" value="GH15225P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12606">
        <property type="entry name" value="SENTRIN/SUMO-SPECIFIC PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02902">
        <property type="entry name" value="Peptidase_C48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50600">
        <property type="entry name" value="ULP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0788">Thiol protease</keyword>
    <feature type="domain" description="Ubiquitin-like protease family profile" evidence="6">
        <location>
            <begin position="122"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP76531.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="339" mass="38944" checksum="73382ABAB8A69E63" modified="2012-04-18" version="2">MAFRKRELRPDEPEDEDEDDPIDMLSSKSRTSSYMKAGPSRDPSQSLLNPITDLPPAEIEPVTPASPDLDLLFSRFGQLRLAKTKSKSHQRKWPTTLTPAQQIVVDEGLRPNNKTVYDLPGASCGIKELQKLSSPPQWLNDEIINFYGSLINLKSHDQISSKALNVHCFSSFFMSQFDLGGHSSVKRWTRKINLFEKDLILFPTNLSNLHWVLGVINNRKKRFEYYDSLAGRNPDVLSKLRRYYQDEWQAKKSEDVDLTEWSDYHPKVPLQSNSSDCGVFVCQFMYSLSQNLINITSQEGRDVSLFDFSAENMPYLRQKMVLEIIRKSFLPDITPESPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWW1</accession>
    <name>E3JWW1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_02977</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC12A transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76536.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320955.2">
        <property type="nucleotide sequence ID" value="XM_003320907.2"/>
    </dbReference>
    <dbReference type="FunCoup" id="E3JWW1">
        <property type="interactions" value="58"/>
    </dbReference>
    <dbReference type="GeneID" id="10528312"/>
    <dbReference type="KEGG" id="pgr:PGTG_02977"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02977"/>
    <dbReference type="eggNOG" id="KOG1288">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001883_4_0_1"/>
    <dbReference type="InParanoid" id="E3JWW1"/>
    <dbReference type="OrthoDB" id="2020542at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015379">
        <property type="term" value="F:potassium:chloride symporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006884">
        <property type="term" value="P:cell volume homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055064">
        <property type="term" value="P:chloride ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902476">
        <property type="term" value="P:chloride transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055075">
        <property type="term" value="P:potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034486">
        <property type="term" value="P:vacuolar transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000013">
        <property type="entry name" value="Solute carrier family 12 member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018491">
        <property type="entry name" value="SLC12_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004842">
        <property type="entry name" value="SLC12A_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827:SF72">
        <property type="entry name" value="GH08340P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11827">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03522">
        <property type="entry name" value="SLC12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="142"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="174"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="277"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="310"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="410"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="503"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="530"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="566"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="591"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="9">
        <location>
            <begin position="147"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="SLC12A transporter C-terminal" evidence="10">
        <location>
            <begin position="665"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="79"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="996"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1039"/>
            <end position="1120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1133"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1217"/>
            <end position="1277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="80"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1048"/>
            <end position="1065"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1066"/>
            <end position="1082"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1157"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1230"/>
            <end position="1239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1249"/>
            <end position="1268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010593"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP76536.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1369" mass="150132" checksum="9A497647489D5FF3" modified="2012-04-18" version="2">MPTNQQPPQSSSSNPVPQSQYGTLDSTQHAWIDKAPSSPTHFLSRASRRLPELQQQLQLNPELTANEQADGHLPTEREPLLSYHSSKPSSPLTSPSTQQAPPQAHSANQHRSGSNDSLSTSHSNSHQQALSTNSRPLPAKKLGTFDGVFLPTILSIFNVVYFMRFGYCIGQIGLLGTVCLLLIAYLINILTVFSLSAIATNGQVRGGGAYYLISRTLGPEFGGSIGILFCLSQAMTAAMNIIGFVEAMITITQLHLYRSHQDTVNGDPQIQNTSTSVIRYFLKTAALLGATFACIVLGKQKIFCSMTRSVSLLLLFTLLSMLFSFINKSPFSDPVQHLNYTSFSIATFKKNLYPDFQFLDDGSSTKIQVKFSDYQRVFGIIFPSLSGILAGSSLSGELGKPSKSLPSGMISALISIITVYLVTLVCLSLTCAREAFTVPNQLGFLNLIVLQISSYPALMSLGLLLCTLFSSIMGITVCGKILQAVSRDGLIPIMKPFFSQGSTIGDDPVYSILLCFIVCQIALFCNVSQLAIHITTISLLVFACINLACFTLRVAGSPNFRPSFRLFSEWTALLGLALALLSMYLVGPVSASTSIIAMITLFVVIHYSSPAKQWGEVTQSIIYHQVRKYLLRLDERKDNVKYWRPQILLFTNDPRHDWNQIVFCNSLKKGGLYVLAHIIKSEFSPEAIKELQEQQLNWLGLVDISNIKAFVDLTLAPDERVGARQLLLTAGLGGMRPNICILGFPTNLKWRGKGKLVDNQTGSPTPLLAKRRSDSSITVRGMVIESTADISIDCLGSLPTDSQRSETPIKPTDFVGIIEDTLSLNKSIGLTYNFQGMRLPESISRPQTVYSNDHMGPPADGSFQGSNPDKGKRWIDLWPILRAGPSAWETYTMVLQLGMILSMVPSWRHYHNLRVTIFCEYDQEIAEERRRMEKLMSDLRIRATLRVVVLANGQIESYECLVKGKTVDMVAWSKIERTLAGDPWWETLKFMRAQDYPPQSEASTPSTSRERSSSRSDSVCSASLDRNLLKKNAQTNNIRIKALHPLSRRTSNQTSQSAQSGSTATGDDDDDLILEENDGDDDTIAREAYNRNKKPVGLPDDPVGVQSKPATPRISIPNYGSTFEHQSLDLQSLSPSSLHNDIPSPPPQKRGNMSRAGSGSSVVSSSSSSSISVKLDNWIRSPGSFNPSHRNKSSQGLASVLHRKKLFSKAPPEMVVEETAGQAEQHQRKPQGSSSSTSGKMKDRTPYLSSSSGGASTRSSSSSSCPSDNLPPTPPPSFLPPVVELDFNSLPMHAQLICLNELIRFHSDHSNQHSSSTAIIFTSLPPPESGTSSSFEGSARYLDQLETFLKDLPPVLAIYAKQFTVTASL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWW6</accession>
    <name>E3JWW6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02982</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76541.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    <dbReference type="KEGG" id="pgr:PGTG_02982"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02982"/>
    <dbReference type="HOGENOM" id="CLU_150829_1_0_1"/>
    <dbReference type="InParanoid" id="E3JWW6"/>
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    <dbReference type="Proteomes" id="UP000008783">
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    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76541.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="105" mass="11250" checksum="59A23706CA23002F" modified="2012-04-18" version="2">MHCLFYLACFLAVLQSALAVPTIAPRADTTATKGHSDEKCWGVYSYSCFGGGMYSFNSLSYPWSLYGASAYLPYNSLLGYPGYPGYGGLWKKDASDAGKTPSSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXT1</accession>
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    </protein>
    <gene>
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76856.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="AlphaFoldDB" id="E3JXT1"/>
    <dbReference type="GeneID" id="10529041"/>
    <dbReference type="KEGG" id="pgr:PGTG_02317"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02317"/>
    <dbReference type="HOGENOM" id="CLU_154754_0_0_1"/>
    <dbReference type="InParanoid" id="E3JXT1"/>
    <dbReference type="OrthoDB" id="2508403at2759"/>
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            <begin position="20"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76856.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="102" mass="10298" checksum="13F591094490B831" modified="2012-04-18" version="2">MRCSLVIALPIALLSSVSAEFFLRRGLDSLPIVGGLLGGSPKSGDGDKNSCADQQSLLANVNVLSSVHCSSGAGGPDLMACGQSGNSYHDGAYHTSADCANN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXX3</accession>
    <name>E3JXX3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02359</name>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
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            <taxon>Puccinia</taxon>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76898.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003321269.2"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02359"/>
    <dbReference type="HOGENOM" id="CLU_095050_0_0_1"/>
    <dbReference type="InParanoid" id="E3JXX3"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        </location>
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    <feature type="chain" id="PRO_5003173346" evidence="1">
        <location>
            <begin position="24"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76898.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="255" mass="28496" checksum="4CB6DBF24EFC919A" modified="2012-04-18" version="2">MSAYPKLSMTIALVTLLAGKSLAIECYPGPVPHRPVLREECSKAISQIVYKPDQTLDPASKQVDYSYGDCNISVVNQFGIRINKAQVEHRFNEIFDKCPHNTGGIDVDQIVFYANKRSIDGYQSWDSDFPARLPVCALADRAPRLTQNDCTKAFSDIPTDSHGRIVDQDGRRTHSVQLTNKTCTLTVYTFDFSKLDVTKAQLEDDFLKTLEHCDRKCGVVRIQGGAEGPNGRVYLSFRHASADKSCTIPFLPFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3JY25</accession>
    <name>E3JY25_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_02411</name>
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    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
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    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <dbReference type="KEGG" id="pgr:PGTG_02411"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02411"/>
    <dbReference type="HOGENOM" id="CLU_1289511_0_0_1"/>
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    </feature>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77489.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
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</entry>
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    <name>E3JZS8_PUCGT</name>
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        <recommendedName>
            <fullName evidence="6">MARVEL domain-containing protein</fullName>
        </recommendedName>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
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                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
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                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
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                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
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                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
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        <source>
            <dbReference type="Pfam" id="PF01284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
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    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78993.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323412.2">
        <property type="nucleotide sequence ID" value="XM_003323364.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3D6"/>
    <dbReference type="GeneID" id="10530814"/>
    <dbReference type="KEGG" id="pgr:PGTG_04949"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04949"/>
    <dbReference type="HOGENOM" id="CLU_2122257_0_0_1"/>
    <dbReference type="InParanoid" id="E3K3D6"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP78993.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="114" mass="12925" checksum="9944FB2787BC87DC" modified="2012-04-18" version="2">MEGEAPAEGEPGLLDPLKRRAITRKLQFLIYKHRPTNPERKISITKIQWYLILWCVPAVYMYVDRKDDNLIKDAPLKGRPTVIVQQAQPMVTCALIGIQINMFTNQGVSFEAGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3Z9</accession>
    <name>E3K3Z9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">choline-phosphate cytidylyltransferase</fullName>
            <ecNumber evidence="8">2.7.7.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_04757</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cytidylyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.15" evidence="8"/>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78801.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323220.2">
        <property type="nucleotide sequence ID" value="XM_003323172.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3Z9"/>
    <dbReference type="STRING" id="418459.E3K3Z9"/>
    <dbReference type="GeneID" id="10536821"/>
    <dbReference type="KEGG" id="pgr:PGTG_04757"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04757"/>
    <dbReference type="eggNOG" id="KOG2804">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037448_0_0_1"/>
    <dbReference type="InParanoid" id="E3K3Z9"/>
    <dbReference type="OrthoDB" id="17102at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005635">
        <property type="term" value="C:nuclear envelope"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004105">
        <property type="term" value="F:choline-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031210">
        <property type="term" value="F:phosphatidylcholine binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02174">
        <property type="entry name" value="CCT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000172">
        <property type="entry name" value="Related to choline-phosphate cytidylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041723">
        <property type="entry name" value="CCT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045049">
        <property type="entry name" value="Pcy1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10739:SF13">
        <property type="entry name" value="CHOLINE-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10739">
        <property type="entry name" value="CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4 11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="6" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="10">
        <location>
            <begin position="277"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="126"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="440"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="42"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="59"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="127"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="199"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="468"/>
            <end position="477"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP78801.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="477" mass="51708" checksum="CE93C2C02498CE2C" modified="2012-04-18" version="2">MLQSPPKSTSSSAPVPPPVIVKRRRKSGKGQGESSREASAESNDETATDNDPMADDTDTASMTSPRNPSNQFRNRTAFEESHHSLAPPHRHKRPQTLAATATINNSIPLLGKDDTSPLRISAAGLSHQASLESGLDSSTSTYDGDVSSAPGRADKRGHTTVIKHPQAAQSDDASSESKAGSGPGSALSHMSWAHENHPQQHSHSQLAPPNKANTTPRSQSLTTKPLNPNLPPAPHGILSRIAPEDLQAEMKEAIQNSSKQRTYSINPPPTDRPVRIYADGVYDLFHYGHALQLRQCKLFFPEVYLMVGVCSDELVRKYKASPVLTSAERYESVANCKWVDQVIEDAPWQVDAAFFEKYQIDYVAHDEEPYVSVNSDDVYAYAKSIGKFLPTRRTDGVSTSELLQRIVGGYIEGTYDNKLEKIGAGDLCSNIAFSDTGSGAHRGAGCRTPMVPRSPVHQTDAEMDDGTTAGNDPAQQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3K5Z3</accession>
    <name>E3K5Z3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_05885</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP79564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003323935.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="10531395"/>
    <dbReference type="KEGG" id="pgr:PGTG_05885"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05885"/>
    <dbReference type="HOGENOM" id="CLU_124024_0_0_1"/>
    <dbReference type="InParanoid" id="E3K5Z3"/>
    <dbReference type="OrthoDB" id="2505965at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000612">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051834">
        <property type="entry name" value="RING_finger_E3_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931:SF3">
        <property type="entry name" value="RING ZINC FINGER-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931">
        <property type="entry name" value="SI:CH211-59O9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="125"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP79564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="181" mass="20953" checksum="F7ED641A8F98BD1B" modified="2011-01-11" version="1">MTDAALAPLGPEDRDLFKLFIASLQEAYGDLYRDPLRTRFNAEEQAHNSRFVDQVDDLLERLERKVAGPLFDVWLYWIRVIDELEESRVLSRRKRRILVEERLDALSDTTPATLPSNPDGEALDCTVCIDELSNPEKSLIQLPCHPSHLFHRDCIQQWLEGHLGCPICRVEVELPPWEYPC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K860</accession>
    <name>E3K860_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06390</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80434.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324853.2">
        <property type="nucleotide sequence ID" value="XM_003324805.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K860"/>
    <dbReference type="GeneID" id="10539443"/>
    <dbReference type="KEGG" id="pgr:PGTG_06390"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06390"/>
    <dbReference type="HOGENOM" id="CLU_095050_1_0_1"/>
    <dbReference type="InParanoid" id="E3K860"/>
    <dbReference type="OrthoDB" id="2498688at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171804" evidence="1">
        <location>
            <begin position="19"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP80434.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="257" mass="27868" checksum="C2D176D8267070C7" modified="2012-04-18" version="2">MALIQFLVIALLGLQVLAASPPKKPVKPMDPTVPQCYTEHELMGEVQKADCPAALAKIIYEKDTLSKLETRLAVVSGDCVIRLNNTKQVVVTKKQIEDTVTELTKSSCKSGHAKVKGNAEVSISITARGKPRAWFTPYDPDFPLGKPFCFRAAQGQVVKEDCLEAFNQLPVDEQGQLVSPADKKIGHTFNLKVKSCRVIVYTTDGSNVSVKKQDISAVVTGMINNCNSQWGTVNVKGTEGPNGQGPNGHVTIVTRSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3K8C4</accession>
    <name>E3K8C4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Oligopeptide transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_06690</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80734.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325153.2">
        <property type="nucleotide sequence ID" value="XM_003325105.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K8C4"/>
    <dbReference type="FunCoup" id="E3K8C4">
        <property type="interactions" value="70"/>
    </dbReference>
    <dbReference type="GeneID" id="10539753"/>
    <dbReference type="KEGG" id="pgr:PGTG_06690"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06690"/>
    <dbReference type="eggNOG" id="KOG2262">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004965_2_1_1"/>
    <dbReference type="InParanoid" id="E3K8C4"/>
    <dbReference type="OrthoDB" id="9986677at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035673">
        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004648">
        <property type="entry name" value="Oligpept_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00727">
        <property type="entry name" value="ISP4_OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22601">
        <property type="entry name" value="ISP4 LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
        <property type="entry name" value="OPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="373"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="472"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="504"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="528"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="567"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="603"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="641"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="708"/>
            <end position="730"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="768"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="795"/>
            <end position="817"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="844"/>
            <end position="864"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="876"/>
            <end position="895"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="948"/>
            <end position="966"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="1024"/>
            <end position="1045"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="249"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="13"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="36"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="69"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="79"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="96"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="113"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022856"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP80734.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1088" mass="122254" checksum="48F46A94105905CC" modified="2012-04-18" version="2">MAYGAGIPPGSNRPRTAASTIRRPTTAVSRNTNNQLDDDLYRDEEWDDGMTEGDQDDRRESEDDDENDDGRRRDSGRIEEEEEEEEDEDEGMFSFAPPPENSITNPLPPIQSPPSIYQPYQPQQSQQQQQQQQLHHQHQQQQQLPHLHHQPNSQSFIQNSQSYSASNSAPSNHPYPPTQQPTSITPAAAIIAQYHQNPETRLRKLTNGTPPSSLNPRAFSSAIPLKTANSVDEAPVIELRTGFTAASRRAAAVQDGDRDSVKSGSSHSLHKLDYYHSQSQPNSAYRPDKQFNSSIINPDDPNSFGLSSLGMKELDRGSMGEVKLYSDTQSHLSQGLDGYNMEDFEMDEEDSPYPEVRASVSNIDDPEMPCLTFRAWMLGLFFVVICGALNMFFQLRYPAPFITPVIVQIISYPSGKLCATILPDRVYRLPGGEGLKRSGLKGFLDRIWPRWLGAGAEWSLNPGPFNIKEHTVIAIMANAAVGPVYAVNMTLVMEKYYQRPPGPGFDICIALATQLLGFALAGVTRRFLIWPASMIWPSNLVVCTLLNTFHAEDDDGSDGSLTRFRFFMYVAVGSFVWYWIPGYLFTALSTFSWVCWLAPRNRLLNQMFGTVNGLGLSLLTFDWSQIAYIGSPLVVPWWAEVNVIIGFIFFFYILGPVLYYTNTWSQAYLPLGGAGVYDRYGQPYNTTRVVDLKTGTLNLTAYREYSPLFLPTTFTAVYSIAFALATSAIVHTALYHGHSIWDKIKNIKTEDEDVHAKLMRNYPEVPDWWYWSYFIVFAAFSMIIIEVFDTGLPIWGLLLALFIALAYVLPGGFIFAMTSQQISINLVAEIIPGYLLPGKPFSNMLFKTFSVQSLLVGLAFIQDLKLGHYMKIPPRVTFAVQIVAAILSAFVQIGVKKFLVATVPDLCDKHQANLLTCPNTSVFYSASVLWGLLGPHRQFGEGTIYRPIVYFTLIGAVLPIPFYLMTKRFPNSWLKYANLPVLLLGACWIPPATPINYTSWILVGFIFQYVLRRMKFRWWSKYNFVLSAGLDVGTVIGAIVIFFTLQLPRGGQLGTLNWWGNTVYLETDDFQGTPYLDPPAEGFGPTTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3K973</accession>
    <name>E3K973_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Cytochrome P450 52A3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_07100</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP80848.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325267.2">
        <property type="nucleotide sequence ID" value="XM_003325219.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K973"/>
    <dbReference type="STRING" id="418459.E3K973"/>
    <dbReference type="GeneID" id="10539878"/>
    <dbReference type="KEGG" id="pgr:PGTG_07100"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07100"/>
    <dbReference type="eggNOG" id="KOG0157">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_27_2_1"/>
    <dbReference type="InParanoid" id="E3K973"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24296">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP80848.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="474" mass="53908" checksum="350EB0CDBBD54317" modified="2012-04-18" version="2">MTLKLRPGFSITLPGARVIEISKPEWIEYVQKTNFENYVKGDMFQSLMADLFGKSILVTDGAAWKRSRLVTSRIFHINTFKIVVEPVVDQSMHEVLEVLQVAGDEGRDIDFCNLFNRFTLDLFAEMTFGTKLGLLEGNSAVKDEGGEGYSYPEAFCDAFNFSQKHMDSRFDVAVVWQWIQKMNFRATKKMESSCHTIHDHAYTAIDEKMSKLSTGDLTADDTLQKDFLGLIMLSHLQKGHTLTRDELRDDLLSLLFAGRDATAQSLSWCFFHLLMNKDIITRIREEAAEILGEDSENVGRVTPDNYRLFTCAYASLLEAFRLHPAVPKNVKFAKANDKIPEGPMIEAGDCLTWSDWQLARDPEVWGPDCGQFIPDRWIDETGNITHFGNFKFHSFNGGPRLCVGMNMAIYIEVKTIVETLQKFDLEFSQGWLENVPMSEEIPGVKTLYPTPQYQPSLTLPMKNPMMISATPRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>E3K9R0</accession>
    <name>E3K9R0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">YTH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_07186</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP80934.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325353.2">
        <property type="nucleotide sequence ID" value="XM_003325305.2"/>
    </dbReference>
    <dbReference type="SMR" id="E3K9R0"/>
    <dbReference type="STRING" id="418459.E3K9R0"/>
    <dbReference type="GeneID" id="10536092"/>
    <dbReference type="KEGG" id="pgr:PGTG_07186"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07186"/>
    <dbReference type="eggNOG" id="KOG1901">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_254230_0_0_1"/>
    <dbReference type="InParanoid" id="E3K9R0"/>
    <dbReference type="OrthoDB" id="306690at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990247">
        <property type="term" value="F:N6-methyladenosine-containing RNA reader activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061157">
        <property type="term" value="P:mRNA destabilization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="YTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.590.10">
        <property type="entry name" value="ph1033 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007275">
        <property type="entry name" value="YTH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045168">
        <property type="entry name" value="YTH_prot"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357:SF89">
        <property type="entry name" value="YTH DOMAIN-CONTAINING FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12357">
        <property type="entry name" value="YTH YT521-B HOMOLOGY DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1170"/>
            <end position="1305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="87"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="313"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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            <begin position="480"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="537"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="671"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="750"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="956"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1324"/>
            <end position="1379"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="998"/>
            <end position="1025"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="23"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="40"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="87"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="146"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
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            <end position="194"/>
        </location>
    </feature>
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            <begin position="332"/>
            <end position="342"/>
        </location>
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        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="2">
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            <begin position="537"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
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            <end position="780"/>
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    </feature>
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            <end position="984"/>
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            <begin position="1327"/>
            <end position="1339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1348"/>
            <end position="1364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP80934.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1405" mass="151458" checksum="9AC66064F3FF260B" modified="2012-04-18" version="2">MLKLPSVSLPFGKRSQTKPHNRSSPSSPNPTSQSAASLPNDASPSHNRDSSSQLSVATLPANSDHTSTPSVSLKRRYQLNFKHLQTSLTHTSPSTSNSNHQATASLQAAASSNSDSDINQPVSTNYYPSNNPPASSPHHSNLPKDNLQSNTLHHQNTNKRASSNLTQTNPTFSTTSTNTNNNNNNNNNNPNENNIHTAQSMPASHHLYPDHSASHLIPPSAISSPLSPIGSPAIAMSYNHQQLSLLRRHHTLSAGGPRLARLEKQQALANMAEKDRRFYGGGSSEAGSSLSGSESVESLSLDNRSNLCLTPLQSSQQHSPSVMPELSDHPLLHKTHHHHHPHQPSSGSNANNSPPSTRNALVATAPHSGFQLIRGYKGTAALRSLDLGSPIELEEVDEEHRLNNSTSHDTISYCNSLQSAPSQAHQLGNQLDPTTTVSVHTTNSRTPTIEARSGTRAADQIPHSGSFPLYSQVALHRNFSSPSKLQQSTTAFSNSDRSSNSTEQAHSFCPPPNWNKLSILNRAMKPNDSALQLLHTLQPTRSQTQGEPSRSLGLTTEEEDDQQILSSPVDFPQTSNLHRATSDRVSSTGNHLNASQPPSSNTLSHWAEQTNLRRHQSMKSDGRWGTSAVTNFEDSETRSANESNQLKESARNQLDTFYELSSSIAGQSASLLRSSSHHSNPSPAFSSSMSSHPSPSGNITQFSTQTGGVVTTMSSPVSVPGLSINPWSPSVEEAKKLREPMLRQASLDTLASNNSSNNSHPMKKSTNSDLSRFRSSSNDDTQGRGEMNLLHTSFGRINLSQAKNGEELSARPAMGLSHRISGVKSPLSTSTSLADLSEGTAADAEVANSVVVAPSSKKLALPPLVTDFSRETILAGGVQGRKPPGSIKRHPNGVSLLFQGPASAAAFVPPIGHSLSRNINDPFNFQEKVSMVASHNTAAPGIGASAWLTQKERLVGETQSRQQSGEWGSLPHSPSSQLQTATTSVPNLPQQIQQQIILNQQLQQLQQLQVQQHQLQQQQTQLLNSTMRLPGSSTSSINGFNSPAMANILQSNAFSYSNMSPSAPGSFFGASGNHHVGPTPPPNPSRLQTTITGLPGLGSLSGTSPHVLPSPVSGLGPMAAGPSMVNMAKPMSFMNPNPAGLPGSNNHNIHELARQKGYNPTHFDTSPPSARFFVIKSYTEEDVHKSLKYEIWASTDLGNKRLDRAFHESHESGPIYLLFSVNASGHFCGMAEMLTAVDYNTSSKVWAQDKWKGIFKVRWVFVKDIPNNALRHIKLTNTPENKPITSSRDTQEVPHDKGIQVLHIMSSYQSRTTLLQDYAWYEHNESQKSQQQQQGSESSPGAAANSGSESTNKSSPPNSSTGPTAPRQPRHSVQNTQNQNIRRFTQANGHPGQYAPVAVQVASGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KD07</accession>
    <name>E3KD07_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07668</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82271.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326690.2">
        <property type="nucleotide sequence ID" value="XM_003326642.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KD07"/>
    <dbReference type="GeneID" id="10529644"/>
    <dbReference type="KEGG" id="pgr:PGTG_07668"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07668"/>
    <dbReference type="HOGENOM" id="CLU_1548363_0_0_1"/>
    <dbReference type="InParanoid" id="E3KD07"/>
    <dbReference type="OrthoDB" id="2509557at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82271.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="173" mass="18856" checksum="95ADCDE42D442306" modified="2012-04-18" version="2">MVEANSTRATSKKIKESTTGKDKLEEIAITKALTPTGGNTDAMKSNERVEDTNKAPDGNDQALSGNSQFVARDNPRANDTSKSRRDQAPVAMNIPVPANAANNEIRKEQASTSFDRPIVNSRDGRIESGYPRGCGYPLTISAETHIRIRRRIPASVGGYPRGYQRGYPRIPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3KEA6</accession>
    <name>E3KEA6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="4">30S ribosomal protein S17</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_08835</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82639.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327058.2">
        <property type="nucleotide sequence ID" value="XM_003327010.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KEA6"/>
    <dbReference type="GeneID" id="10530022"/>
    <dbReference type="KEGG" id="pgr:PGTG_08835"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08835"/>
    <dbReference type="HOGENOM" id="CLU_073626_1_2_1"/>
    <dbReference type="InParanoid" id="E3KEA6"/>
    <dbReference type="OrthoDB" id="274752at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_uS17"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP82639.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="91" mass="10014" checksum="D429244072C8D7F6" modified="2012-04-18" version="2">MSLLAATKTIVKPVVGVVIQSGVQPKTVKVLRTTQVLHRVVPKAIKKTVVYTVHDEKNSARRGDKVEIIPAGRRISTNKTYALSRILPSTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>E3KF02</accession>
    <name>E3KF02_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">FYVE-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_10002</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178284">
        <property type="protein sequence ID" value="EFP83034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327453.1">
        <property type="nucleotide sequence ID" value="XM_003327405.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KF02"/>
    <dbReference type="STRING" id="418459.E3KF02"/>
    <dbReference type="GeneID" id="10544746"/>
    <dbReference type="KEGG" id="pgr:PGTG_10002"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10002"/>
    <dbReference type="eggNOG" id="KOG1842">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028545_0_0_1"/>
    <dbReference type="InParanoid" id="E3KF02"/>
    <dbReference type="OMA" id="HFEANHA"/>
    <dbReference type="OrthoDB" id="166134at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15737">
        <property type="entry name" value="FYVE2_Vac1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.20">
        <property type="entry name" value="Rabenosyn, Rab binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052727">
        <property type="entry name" value="Rab4/Rab5_effector"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021565">
        <property type="entry name" value="Rbsn_Rab-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036531">
        <property type="entry name" value="Rbsn_Rab-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13510">
        <property type="entry name" value="FYVE-FINGER-CONTAINING RAB5 EFFECTOR PROTEIN RABENOSYN-5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13510:SF44">
        <property type="entry name" value="RABENOSYN-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11464">
        <property type="entry name" value="Rbsn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140125">
        <property type="entry name" value="Rabenosyn-5 Rab-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="7">
        <location>
            <begin position="176"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="418"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="516"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="15"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="429"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP83034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="545" mass="61238" checksum="9CF670F0378AA667" modified="2011-01-11" version="1">MNQQQQQQHYRPYTPSSASASPSPSNIKRNNSPASRTTTTTPTDTPTNRSPSNLKLNQVPFRSGFQPKGVYRDRTEELITARNSRQESKRLEEARLQKRLEKLIQLHFPAENNQQSATQDGQQDQNQPSLINLSQKLIGIVKHVGHDRGEFGGINLVGQNGSIRAQEQRIVRWQEDGEVKECKYCQTAFGLRIRKHHCRLCGSVVCFSPPWTTGQRERCSTMIRFEWERATESRTEKGIHGRIIQLSEQEMGIVDDPHSVLDLISSSSTNPVPAHIPPKPRKGVRVCSHCLAIILRRQAMTYPPKIPDYIHLYASLKRIQDEIEKAIPDFQELLSSSKHPNSNLNPSSTSDLPIKLLTIRRKLLTNLSLFDAHSKKLIGQVARREGENGSEARLVSAITTRAGLFLRDHMSLIRSLGLLETQPTPQPKPSSSTTTTTMRATRPRPSSSSSLPTSLPQPNPLASTPPNKLHIKNLSISSETNHSIHSQEDTHHILVQLNVLLEQENRIEGFLALAQSKRQLEDVASLKSSLDDLREEIANLKKSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KF22</accession>
    <name>E3KF22_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_09821</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178284">
        <property type="protein sequence ID" value="EFP82853.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327272.2">
        <property type="nucleotide sequence ID" value="XM_003327224.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KF22"/>
    <dbReference type="GeneID" id="10544563"/>
    <dbReference type="KEGG" id="pgr:PGTG_09821"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09821"/>
    <dbReference type="HOGENOM" id="CLU_109042_0_0_1"/>
    <dbReference type="InParanoid" id="E3KF22"/>
    <dbReference type="OrthoDB" id="2507202at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP82853.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="145" mass="16848" checksum="63BFB23D1D7C93A6" modified="2012-04-18" version="2">MAYHHNEATEEILDGVMNLPSIPKFGMILFPSNPSLQFVWEMFFNDKAECADILEELLSRRYLSLRTAPTTHDDYDLAKLFNMRNDDFKQAVRTTKDGFLWLLNQIRTHPIFYSNSARPQLPIPHQLALTFERLGSNGVFNLDKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KFV1</accession>
    <name>E3KFV1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_09189</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EFP83236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327655.1">
        <property type="nucleotide sequence ID" value="XM_003327607.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KFV1"/>
    <dbReference type="GeneID" id="10544949"/>
    <dbReference type="KEGG" id="pgr:PGTG_09189"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09189"/>
    <dbReference type="HOGENOM" id="CLU_855647_0_0_1"/>
    <dbReference type="InParanoid" id="E3KFV1"/>
    <dbReference type="OMA" id="EENECFQ"/>
    <dbReference type="OrthoDB" id="2497107at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP83236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="325" mass="36531" checksum="B12BA550D0BE9307" modified="2011-01-11" version="1">MESFSMPPIPCMISLGSERALQRDDPQGDYLDRVNQHRGLPIYDSKKNDSFYEDSPAFEMPRFYSEEEGDDTQILPSQKYAPSMPVADKSGHVDTGLPCFSSESNLPVKPPAASLPARSTRKRSLTNLFKAKPETIILAMKTELNPPGIEPPVATSVPRTCSTPNETNSQISALIDEQASYLKRIVLAEPNVGTIPSSICQRLNFCQREEEIFYENEVLAGSSLLSTAQVIQILNQYKPSHEASQASISKAAHLSQARTNLKTRSVNRSITVGYQPCKEGFTAKDENIYYQRIWKHLENRVEAHDRMKCTLEMISEEEENRENLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KH35</accession>
    <name>E3KH35_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09323</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328029.1">
        <property type="nucleotide sequence ID" value="XM_003327981.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KH35"/>
    <dbReference type="GeneID" id="10532294"/>
    <dbReference type="KEGG" id="pgr:PGTG_09323"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09323"/>
    <dbReference type="HOGENOM" id="CLU_2098042_0_0_1"/>
    <dbReference type="InParanoid" id="E3KH35"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="100"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="116" mass="12510" checksum="EC10473470A28D97" modified="2011-01-11" version="1">MKDVGNVQTEPLSIQLDRIQDDENKWDWAPNHDPQGDRIVRIVGWIVLSGKMYSALFGESGSVHSVNRIELDFRCEMGVGCSFLTLPSSGTLACGDERASPAGSGSPAARATCSLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3KH43</accession>
    <name>E3KH43_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_09331</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328037.1">
        <property type="nucleotide sequence ID" value="XM_003327989.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KH43"/>
    <dbReference type="FunCoup" id="E3KH43">
        <property type="interactions" value="157"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KH43"/>
    <dbReference type="GeneID" id="10532304"/>
    <dbReference type="KEGG" id="pgr:PGTG_09331"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09331"/>
    <dbReference type="eggNOG" id="ENOG502QTX9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009665_1_1_1"/>
    <dbReference type="InParanoid" id="E3KH43"/>
    <dbReference type="OMA" id="FTMPNTF"/>
    <dbReference type="OrthoDB" id="655030at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051704">
        <property type="entry name" value="FAD_aromatic-hydroxylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46865:SF2">
        <property type="entry name" value="MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46865">
        <property type="entry name" value="OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="373"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding" evidence="5">
        <location>
            <begin position="10"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP83618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="420" mass="47010" checksum="556538F17F11FDE6" modified="2011-01-11" version="1">MTDTNPQKRLRVLISGAGIGGLVAAYWLGKAGASVTVLERAKDLRKEGHIVGIRKEALPIINYMGLEEDIRRQMSHEMGLKLVDNNNSTWAAFPPGDLGFSCGVELRRGDLVKSLYERTKDQAHFILGKTINTIEENNESICVTLGDNAEVLQFDVLLVAEGLRSKTRARIFKEKVDTPISSLGLLAASFSYKSEDNWAYWYNIPDHRALLLRPDRFGQTRTLAMCRKSEEYTTSTNLVSSKTPVEQQKDFFIRRFKQTGWEKTGKIMRAMNDADDFYVLEMVQVKCKKWSRGRTVLLGDSAYCPSPFGGMGTTAAIIGAYSLAAKLVQTPGDHRSAFESYENLLRPWAESIQKPVAMTIHFGLPGTRMGIRFFHLLGFFVSLPLVSTILPFLIPFLVQLGLPVNAIAPRNPKPSVFQRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KH52</accession>
    <name>E3KH52_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_09340</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83627.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328046.2">
        <property type="nucleotide sequence ID" value="XM_003327998.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KH52"/>
    <dbReference type="GeneID" id="10532313"/>
    <dbReference type="KEGG" id="pgr:PGTG_09340"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09340"/>
    <dbReference type="HOGENOM" id="CLU_138712_0_0_1"/>
    <dbReference type="InParanoid" id="E3KH52"/>
    <dbReference type="OrthoDB" id="10326738at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP83627.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="162" mass="16486" checksum="883B0E1C5956E0C5" modified="2012-04-18" version="2">MATDLPSNAHQSPLPTQLQYIHLTHTLHPQSQPPSQSLIMLCNTVIALSFAFLHSVFAESVSPRGFSDILGGLPTDNLLGGGNLPGGDLFNGLGFGGQPSETSCADQSGLLLNANVLGHTRCSGGGGAGSMSCNGQNGGKFGPVTLPSLANVQALTSVDCTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KH76</accession>
    <name>E3KH76_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_09364</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328070.1">
        <property type="nucleotide sequence ID" value="XM_003328022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KH76"/>
    <dbReference type="GeneID" id="10532337"/>
    <dbReference type="KEGG" id="pgr:PGTG_09364"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09364"/>
    <dbReference type="HOGENOM" id="CLU_1787769_0_0_1"/>
    <dbReference type="InParanoid" id="E3KH76"/>
    <dbReference type="OrthoDB" id="10358883at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="2">
        <location>
            <begin position="130"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP83651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="145" mass="15932" checksum="C20A98CA9B972E34" modified="2011-01-11" version="1">MSIFFAQNSFPLSQGSNFKESPRLRFTAVPRLEQTWPTDRYLTHQNALSFTPHITESASSSSLITLHCQPITSAQVPVFKPCHNQSIMKCFVFAVTLCATVMVAASVKPVKVDVGHPVTPGSDAPLEVTCPRCGAEGHSWRTCPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="85" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIT0</accession>
    <name>E3KIT0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="16">Ca2+-transporting ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGTG_10583</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type V subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84205.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328624.2">
        <property type="nucleotide sequence ID" value="XM_003328576.2"/>
    </dbReference>
    <dbReference type="FunCoup" id="E3KIT0">
        <property type="interactions" value="597"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KIT0"/>
    <dbReference type="GeneID" id="10545465"/>
    <dbReference type="KEGG" id="pgr:PGTG_10583"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10583"/>
    <dbReference type="eggNOG" id="KOG0209">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001828_4_1_1"/>
    <dbReference type="InParanoid" id="E3KIT0"/>
    <dbReference type="OrthoDB" id="48943at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019829">
        <property type="term" value="F:ATPase-coupled monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015662">
        <property type="term" value="F:P-type ion transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07543">
        <property type="entry name" value="P-type_ATPase_cation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.150.10:FF:000056">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1110.10:FF:000058">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000071">
        <property type="entry name" value="Cation-transporting ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1110.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.150.10">
        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057255">
        <property type="entry name" value="2TM_P5A-ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018303">
        <property type="entry name" value="ATPase_P-typ_P_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023298">
        <property type="entry name" value="ATPase_P-typ_TM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008250">
        <property type="entry name" value="ATPase_P-typ_transduc_dom_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059000">
        <property type="entry name" value="ATPase_P-type_domA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006544">
        <property type="entry name" value="P-type_TPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047820">
        <property type="entry name" value="P5A-type_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001757">
        <property type="entry name" value="P_typ_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044492">
        <property type="entry name" value="P_typ_ATPase_HD_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01494">
        <property type="entry name" value="ATPase_P-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01657">
        <property type="entry name" value="P-ATPase-V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630">
        <property type="entry name" value="CATION-TRANSPORTING ATPASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45630:SF7">
        <property type="entry name" value="ENDOPLASMIC RETICULUM TRANSMEMBRANE HELIX TRANSLOCASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23143">
        <property type="entry name" value="2TM_P5A-ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13246">
        <property type="entry name" value="Cation_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00122">
        <property type="entry name" value="E1-E2_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00002">
        <property type="entry name" value="C1.7:_P-type_atpase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00027">
        <property type="entry name" value="p-type_atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81653">
        <property type="entry name" value="Calcium ATPase, transduction domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81665">
        <property type="entry name" value="Calcium ATPase, transmembrane domain M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00154">
        <property type="entry name" value="ATPASE_E1_E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="78"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="111"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="267"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="290"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="472"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1112"/>
            <end position="1131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1190"/>
            <end position="1210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="1230"/>
            <end position="1252"/>
        </location>
    </feature>
    <feature type="domain" description="P5A-ATPase transmembrane helical hairpin" evidence="15">
        <location>
            <begin position="71"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="P-type ATPase A" evidence="14">
        <location>
            <begin position="323"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1285"/>
            <end position="1329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1285"/>
            <end position="1310"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1320"/>
            <end position="1329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF23143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EFP84205.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1329" mass="148065" checksum="BA1900D9635F36DE" modified="2012-04-18" version="2">MSHQDFSRKARLDTNIIIQRQDIDHQKQNTSTRKGPRLVASRARSNLEMSKSGGIKIVSPEIESIVLLRLRPLITHTYVFPFIFAYPLAAYAYFFEYDRYLRSVDGESQEWTFLLCVTVFGGHALSWLATRWNTTIRQAATSFRVNDISKADLVKVIPKPNKGTPAFCPILHSKRTVQNSKSEKNAPAQQETILYIEYQRDHYFYSPTSHSFGLLAYPSDSKPPMSTMTSSNGITSESELALAKEMYGKNTFDIPVPTFLELLAEHMQAPFFVFQMFSVGLWFLDEYWYYSLFTLFMLIVFECTTVFQRLRTLNEFRTMSIKPYQINVYRNGKWGEVISDELVPGDLVSVLRTKEDSAVPCDLLLLRGTCIASEAMLSGESTPLLKESVELRSGDDQLDFLGNDRNSCLFGGTKILQVTPSTSDAVGDKLKTPDGGCLAIVLRTGFGTTQGQLIRTMIFSTEQVTANNYESFLFLAFLMLFAIIASRYVWVKGVERNLKRSKLLLDCVIIITSVVPPELPMELSMAVNASLVALSKYAIFCTEPFRIPAAGRVDVCCFDKTGTITGEDLMVEGVVGVDDKDVLKLVPLNQTGMETTFTLASAHSLVLLDDGIIGDPMEKTTLEAVGWGVNQGDTILPSSNDHTHRAVITIKRRFQFSSLLKRMSTVSSVVTPDRQTKTMVSVKGAPEVIKTMITNVPEHYESTYKYYTRRGSRVLALAYKFINIQGANKINDLLREQAESELIFAGFLVFTCPLKPDAIETLKMLADSSHRCIMITGDNPLTAVHVAKEVEIVDRECLVLDVRENSTDEKDLVWRTVDDQMIIPVDVSKPIDPKILSDYDLCITGVALKQFVGMPSWVDLVQNVWVYARVSPAHKELILNSLRSLGYTTLMAGDGTNDVGALKAAHVGVALLDGSPDDLKAIAEHQRNERLKKVWQTQLNISQRFNQPPPPVPAALAQVYPELVDVHLKALKSGQDARKANPMEKFNLADITSKMADMDEDSEGPPKIKLGDASVAAPFTSKLSNVSAVSTIIRQGRCTLVATTQMYKILASNCLISAYSLSVQYLDGVKFGDYQMTIQGVCMSACFLCISRAKPVERLSKERPQGSIFNTYVVATVLGQFMCHLAALIYITGLCETTSPRTKEINLDAEFEPSLLNSAIYLLSTCQSVSTFAVNFQGRPFREDIKENKPLFYGLLGAAAVAFCGATNFVPEANGWLQLVDMPTSFRMQLCIVMCMDFGGAMLVELIAKFLFSDVRPKGMVIKGIERREARRKVEEKILLEQQNSTAELDSTASDQKASSSTALNSLQDQNHIRNRNPKKSSSQNKHQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJD7</accession>
    <name>E3KJD7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10132</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178290">
        <property type="protein sequence ID" value="EFP84412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328831.1">
        <property type="nucleotide sequence ID" value="XM_003328783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJD7"/>
    <dbReference type="GeneID" id="10545840"/>
    <dbReference type="KEGG" id="pgr:PGTG_10132"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10132"/>
    <dbReference type="HOGENOM" id="CLU_051119_2_0_1"/>
    <dbReference type="InParanoid" id="E3KJD7"/>
    <dbReference type="OrthoDB" id="2498930at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027532">
        <property type="entry name" value="Mdm12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204:SF1">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="275" mass="31390" checksum="1FF16FE5FFAD7455" modified="2011-01-11" version="1">MSTEVIRETKVLLTPNNYALWLLPMEAKLFRSKLLDILEGQEEPDEKEKESWIKRNNDAYAEIITYISQEVLGFVSSALPATPRFNGRGLWKLLKSQYAGDDLTSQTTALDQFLHLTNQKISMSGLILDDRVRTILMLKKLPSDYRSFRDIVSMNYGSESFESVIKKLESYVAQNDLDKKTSTISSTPLQSLLTRSNDQASSSPTLCDHCKKPGHQVNNCWVKYPEKAPKSHSAHMTVQENFNQLNDPTDFSLFRTADGVLHHIDEVKYEGVKYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLD0</accession>
    <name>E3KLD0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein YIF1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_11274</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Has a role in transport between endoplasmic reticulum and Golgi.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Endoplasmic reticulum membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Golgi apparatus membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the YIF1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP85105.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329524.2">
        <property type="nucleotide sequence ID" value="XM_003329476.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLD0"/>
    <dbReference type="FunCoup" id="E3KLD0">
        <property type="interactions" value="316"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KLD0"/>
    <dbReference type="GeneID" id="10527086"/>
    <dbReference type="KEGG" id="pgr:PGTG_11274"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11274"/>
    <dbReference type="eggNOG" id="KOG3094">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047877_2_0_1"/>
    <dbReference type="InParanoid" id="E3KLD0"/>
    <dbReference type="OrthoDB" id="337750at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005578">
        <property type="entry name" value="Yif1_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14083">
        <property type="entry name" value="YIP1 INTERACTING FACTOR HOMOLOG YIF1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14083:SF0">
        <property type="entry name" value="YIP1D-INTERACTING FACTOR 1, ISOFORM C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03878">
        <property type="entry name" value="YIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 9" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="260"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="290"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="17"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="57"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="85"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU368073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP85105.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="390" mass="43351" checksum="EA41A1EAFB91D1D0" modified="2012-04-18" version="2">MFNRNTHQSGPTLQHPKPVPPPFNLPASPPIPDSQTFTSNQNNNNPYGHPTNDRIRTSTASSSDYQRFSSPPATSNELDQPFFSNPSQAPQNNFNQFQNSPPNPHHQHHLQHQQQNPWPNPNPIGIGPSNNFSNPFGVNDMTAQMGMQFGQSAMKAGSEYVEKNLTRYLPLTHLKHSFNVSNLYVFNKIKLILFPWRHKPWSRLVQRSEVSGQVEGYQPPREDINCPDAYIPVMALMTYILLSGAVAGSKGRFDPELLSIAASQALGIIFLEFCCIKLGCYLLNIQGDGAVIDLVAYSGYKFVGIIVSLLASLAGLRSSICWAIFVYVFAANAFFLLRSLRYVILPDPSGQSFGSATTSTVNSTQKSRRIQFLFAISVSQLASMWLLSRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLQ7</accession>
    <name>E3KLQ7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11425</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178294">
        <property type="protein sequence ID" value="EFP85256.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329675.2">
        <property type="nucleotide sequence ID" value="XM_003329627.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLQ7"/>
    <dbReference type="GeneID" id="10527245"/>
    <dbReference type="KEGG" id="pgr:PGTG_11425"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11425"/>
    <dbReference type="HOGENOM" id="CLU_611298_0_0_1"/>
    <dbReference type="InParanoid" id="E3KLQ7"/>
    <dbReference type="OrthoDB" id="10292445at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="417"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="425"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="438"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85256.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="448" mass="52470" checksum="288A752E7D3FB0C7" modified="2012-04-18" version="2">MHPNVKSMLKYYPNFAGTLLIFNRLLGFSNQTPMIAESSTKATAIVRPKYWFPLPQRSDYMSDEIYDLSDWEAGTSLSHMPSHGETSEAKEIKQEERFSQLSFNIVFIPGALNLYWFQSPGDFQSADCVISSLVNHLDLTEKVYKHYKKIKLEPGSFRGNDRRILYYHQLQNSDGTADHVNYLIQKACQYFWEKPEARREEVRTWLEMFAYHSKRHRRHLFMPTQASLLYGLQNLRKVYAEDPLLAPHIDAVLKLIKRRIGITENGPAGEVSFNWPWIQELINPSMGLTDAMKSYREFQEMIKGEEKIISVLHFTRKKKENIYRNFLKRLSKARREKKRSEFTQAVRNVIQEFELSTSQSRLATLALFYHTLTQHKTSRLRREAWTIFQDFEYTHGPGFLSHEARLVEYIERETGFVKTPDHQGTEPSTSQTHFTDSDTTEEPAEDSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KMH9</accession>
    <name>E3KMH9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_11860</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178295">
        <property type="protein sequence ID" value="EFP85504.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329923.2">
        <property type="nucleotide sequence ID" value="XM_003329875.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KMH9"/>
    <dbReference type="STRING" id="418459.E3KMH9"/>
    <dbReference type="GeneID" id="10544056"/>
    <dbReference type="KEGG" id="pgr:PGTG_11860"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11860"/>
    <dbReference type="HOGENOM" id="CLU_1251216_0_0_1"/>
    <dbReference type="InParanoid" id="E3KMH9"/>
    <dbReference type="OrthoDB" id="2506588at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP85504.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="221" mass="24988" checksum="ABFAAB955D35B41A" modified="2012-04-18" version="2">MAQVGEASDKTAVGEEITKLGVRDVLNTSIIDWRYEILAKKDAATAEEVQFVQGLKNLDQGGKEALFSPFFLLKGFDGCADTPVEVLHVFLLGIVKYMLQSFMKSLATGVLPEVMARYKSFDTKGLNVPSLRPYYLTKHYRNLIGKDFKVALQAAPFVLFEYMLADKRLVWSALCQLAPFVFQTHIAEMDAYQISLQQLVRVFIYHLIKSTVQIIQNQEPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNR4</accession>
    <name>E3KNR4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_11695</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178297">
        <property type="protein sequence ID" value="EFP85939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330358.1">
        <property type="nucleotide sequence ID" value="XM_003330310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNR4"/>
    <dbReference type="GeneID" id="10539938"/>
    <dbReference type="KEGG" id="pgr:PGTG_11695"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11695"/>
    <dbReference type="HOGENOM" id="CLU_571252_0_0_1"/>
    <dbReference type="InParanoid" id="E3KNR4"/>
    <dbReference type="OrthoDB" id="2507691at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP85939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="478" mass="55225" checksum="8AD6F7C67EF00CFE" modified="2011-01-11" version="1">MKTLLISYSAFVLTKSTTLVMIPNSNQRTEELSALLTHKLFDLNEPATEEPSPEHILSSGSSAHPHVTRPTFDLNLPCRLSGEELPPRMCFQEHMGSKYRDRWAYLKRKGDHQDLGFDQSQSQFLSNQRQMIHLNTARHLPDSSVMNELTSARAVVSGNVKRPTHNLRNGFPVGNREQRLNGNLPEPFDVYDWDYVREVPDSEIRDGVGFRLGRPLHQLLMFLDSLRYKPTRDLRFSISDGEERSILRSYQQSKKLLYPPGLTRGARRGALWKIAMSVSNSKIAVDRYDVFSLEIIGSLRAGLTHLGETAADLEYPISPSRINAITGYVENLTKVATFFIIAYLSLFKKHPTERLSTQLVEEILIFLKKFWEDTQNSKSELRKNHEWSTLNAIVLKSEEIDRNPKSYKHWLNGNACYQMAWKMAMYWVKENGKWFGVGLEDEDLYENSIFDLLNKIIIYANYQNPKSFLHYWGDKKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNZ3</accession>
    <name>E3KNZ3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11974</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178298">
        <property type="protein sequence ID" value="EFP86018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330437.1">
        <property type="nucleotide sequence ID" value="XM_003330389.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNZ3"/>
    <dbReference type="GeneID" id="10540020"/>
    <dbReference type="KEGG" id="pgr:PGTG_11974"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11974"/>
    <dbReference type="HOGENOM" id="CLU_970229_0_0_1"/>
    <dbReference type="InParanoid" id="E3KNZ3"/>
    <dbReference type="OrthoDB" id="28947at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="80"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="231"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="261"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="287" mass="31271" checksum="0EE5EF6382358E72" modified="2011-01-11" version="1">MSSSSIDLGKSILAKLQQIQPPKKPNPKTNAGSKRAPPGAGPSSSSSLTKNRSSSDLGRSNTQKTSPRSSDVQKPDKSPSSRKNKKQQKRPSSEVKRDETQAKRPMTKLASPPPDDTSKDHQKSKSKSKHESVDDQEEDNGEEDSEQDDSDDHEILLAEIKNLGGTEDDLDLFEGAPWKEAVIQEEVVVDDAQLVNDVKSFMKGLDFEAALKTLNDRESSPHHPTPSSSVDDSKTKTKDKNTTHPSASSSSSSGVLKRKSSTTTTTNQSSVEPPSNINQKKKKKRNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPD5</accession>
    <name>E3KPD5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribonuclease P/MRP protein subunit POP5</fullName>
            <ecNumber evidence="5">3.1.26.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_12116</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the eukaryotic/archaeal RNase P protein component 2 family.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="5"/>
    <dbReference type="EMBL" id="DS178298">
        <property type="protein sequence ID" value="EFP86160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330579.1">
        <property type="nucleotide sequence ID" value="XM_003330531.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KPD5"/>
    <dbReference type="FunCoup" id="E3KPD5">
        <property type="interactions" value="150"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KPD5"/>
    <dbReference type="GeneID" id="10540169"/>
    <dbReference type="KEGG" id="pgr:PGTG_12116"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12116"/>
    <dbReference type="eggNOG" id="KOG4639">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086710_3_0_1"/>
    <dbReference type="InParanoid" id="E3KPD5"/>
    <dbReference type="OMA" id="IVRCPRA"/>
    <dbReference type="OrthoDB" id="24745at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030681">
        <property type="term" value="C:multimeric ribonuclease P complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033204">
        <property type="term" value="F:ribonuclease P RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3250:FF:000016">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3250">
        <property type="entry name" value="Ribonuclease P, Pop5 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002759">
        <property type="entry name" value="Pop5/Rpp14/Rnp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016819">
        <property type="entry name" value="RNase_P/MRP_POP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038085">
        <property type="entry name" value="Rnp2-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15441">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15441:SF2">
        <property type="entry name" value="RIBONUCLEASE P_MRP PROTEIN SUBUNIT POP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01900">
        <property type="entry name" value="RNase_P_Rpp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF023803">
        <property type="entry name" value="Ribonuclease_P_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160350">
        <property type="entry name" value="Rnp2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR023803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP86160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="136" mass="15310" checksum="F9147C2CD6D786A0" modified="2011-01-11" version="1">MVRFKNRYLLIQLIYGPHESSTIGSNHQSGPSGTKQVNEKSLIDLIRQSIQLNFGDLGAGEAGADLTVKYYSPTTSNLILRCKRDQVTKVRASLLFINQINPQTPVIFNVIHVSGTIRKTQSFLIEFDRQQILSFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPF4</accession>
    <name>E3KPF4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_12135</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178298">
        <property type="protein sequence ID" value="EFP86179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330598.1">
        <property type="nucleotide sequence ID" value="XM_003330550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KPF4"/>
    <dbReference type="GeneID" id="10540188"/>
    <dbReference type="KEGG" id="pgr:PGTG_12135"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12135"/>
    <dbReference type="HOGENOM" id="CLU_1750609_0_0_1"/>
    <dbReference type="InParanoid" id="E3KPF4"/>
    <dbReference type="OrthoDB" id="10341392at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP86179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="149" mass="16031" checksum="A80440D9B8488D1C" modified="2011-01-11" version="1">MEKGMIQSPDWYLSGIGLKCHPIPCIGIGLISAPIPIYQRIRISWSDPIQAAWELGIGIAPADPIKPRYCICIRGFNPRLGRSSFPTDPWSGLATYHLQVLTVTLSVLLPPTPSPCRQLKTPHHHPVALTLLGVGLSLPGPASLSSVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQQ9</accession>
    <name>E3KQQ9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sfi1 spindle body domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_13016</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178301">
        <property type="protein sequence ID" value="EFP86634.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331053.2">
        <property type="nucleotide sequence ID" value="XM_003331005.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQQ9"/>
    <dbReference type="STRING" id="418459.E3KQQ9"/>
    <dbReference type="GeneID" id="10540896"/>
    <dbReference type="KEGG" id="pgr:PGTG_13016"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13016"/>
    <dbReference type="HOGENOM" id="CLU_012263_0_0_1"/>
    <dbReference type="InParanoid" id="E3KQQ9"/>
    <dbReference type="OrthoDB" id="1933281at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP86634.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="766" mass="91399" checksum="4C4CF61FC0FA2716" modified="2012-04-18" version="2">MQAFNHEILSIATGPAAPLFPPLNQEEREILSQIIKLTPPNTNAFNALLSPYENTLRRYRIDPKIDDKYYTFLLKLSLVPGSDWKQKWARVLSDQAISSGLPPKQAIVPTATQVHPTAHDPRRIKQTVYSGTQDKEKWKAINSNVKPATHQTAAHSKSRPPKSVHFLPSKAEENDHNQIKEPPAARDLSLPVELQSEIIQSKMTLLIDPIDVKARKFRREQLLARCMNKWMNSIIYWNTLNSEAQSARRVLDINQWRKLTVSRTIQKKSLALRNAYRKTKGTRERNLGRQVLIWFQEALLRSKLRNFLHGRDSQVKHQVFINWKISLIQSRQSHFFLLKSRFHRLLKACSKFRARQRRLEFSETMAEAHGTRSPYSPAAGLQNQFTVQWTVNLANHHLQHWIQYHHALIQHSAQAQHFHKLHAIRESFLAWTFRHWRTQQLQTEASSTRIYFLRKRVLIVWKRRIQRIRVNLWRQEKTLAHLRDRFTTWRLLFHKRRRLSISLWTFDQRSAERQCMNAISQWRIRLTLTIRNEQTAVEFAQRNLIQRFWVTWTDRHQLIRVLIHESSLVVHRRCQTQKSTLLAYWLDRTRIKVAHNFKLAEFSNRRRRAIVMCVWEKWRDKIRSRELEPIEQTFNFMRDRNSKTQIMQVWIARSSLLFLIGQKKQNTLIDCFKLWYNLTQSHKLRNRFNLRKNRLILKFFFSLWCKKSIDLKTMKIISRFKNNLRIAQPTTRNTRRLCAREVTEEDYTTTTSLTNSSSNETYETSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQV3</accession>
    <name>E3KQV3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_13060</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178301">
        <property type="protein sequence ID" value="EFP86678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331097.1">
        <property type="nucleotide sequence ID" value="XM_003331049.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQV3"/>
    <dbReference type="STRING" id="418459.E3KQV3"/>
    <dbReference type="GeneID" id="10540940"/>
    <dbReference type="KEGG" id="pgr:PGTG_13060"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13060"/>
    <dbReference type="eggNOG" id="KOG3764">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_51_2_1"/>
    <dbReference type="InParanoid" id="E3KQV3"/>
    <dbReference type="OMA" id="TRTPEVW"/>
    <dbReference type="OrthoDB" id="440553at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17325">
        <property type="entry name" value="MFS_MdtG_SLC18_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050930">
        <property type="entry name" value="MFS_Vesicular_Transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506">
        <property type="entry name" value="GH10249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506:SF23">
        <property type="entry name" value="GH10249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="117"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="251"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="313"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="339"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="373"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="410"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="23"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP86678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="456" mass="48450" checksum="9E3A4400130F6695" modified="2011-01-11" version="1">MSPSIISRCYNYFNKVTSEIPYITFSVSFGLMVDMATYGIMVPVLPFRLESTGYKNVPATSSYLIAAFAFGLIISSIPVGIFGEIVRSRKAPLLACLTFLAASLIMFWLSSSFAVLMIARIIQGFSGTAIWTLGLALICDVVPEARIGVIMGYVMIGWSIGTVGGPLAGGLLYDSLGYNSIFVFALIITAIDFFLRSLVQDKKCIQNNQAAAAINVELSDKEVKSTAESSDGSKPPSAARALLSLLTNSRFWTLCAITLVLGFSFGGLLDTGMTMLVKERYGLSSRGAALIFIAAVVPSFISSPMAGHYADKYGAKWPIVICLILGTPFFGLLSMNTSLAALITFIAFTGIFIMGIAAPIMQDLAEVVKATPGLGFAHVYGIFNMVYSTGAMVGPLVVGGLLGRMGIQHGWQIVSLVITGLCAACIIPAWLFLGGKKNTPSQASIPTSDSKLDSPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3KR36</accession>
    <name>E3KR36_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ubiquitin fusion degradation protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_13143</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UFD1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178302">
        <property type="protein sequence ID" value="EFP86761.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331180.2">
        <property type="nucleotide sequence ID" value="XM_003331132.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KR36"/>
    <dbReference type="FunCoup" id="E3KR36">
        <property type="interactions" value="667"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KR36"/>
    <dbReference type="GeneID" id="10541186"/>
    <dbReference type="KEGG" id="pgr:PGTG_13143"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13143"/>
    <dbReference type="eggNOG" id="KOG1816">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037790_1_1_1"/>
    <dbReference type="InParanoid" id="E3KR36"/>
    <dbReference type="OrthoDB" id="422728at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034098">
        <property type="term" value="C:VCP-NPL4-UFD1 AAA ATPase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031593">
        <property type="term" value="F:polyubiquitin modification-dependent protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.50">
        <property type="entry name" value="Ubiquitin fusion degradation protein UFD1, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004854">
        <property type="entry name" value="Ufd1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042299">
        <property type="entry name" value="Ufd1-like_Nn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055417">
        <property type="entry name" value="UFD1_N1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055418">
        <property type="entry name" value="UFD1_N2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12555">
        <property type="entry name" value="UBIQUITIN FUSION DEGRADATON PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12555:SF13">
        <property type="entry name" value="UBIQUITIN RECOGNITION FACTOR IN ER-ASSOCIATED DEGRADATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03152">
        <property type="entry name" value="UFD1_N1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24842">
        <property type="entry name" value="UFD1_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="Ubiquitin fusion degradation protein UFD1 N-terminal subdomain 1" evidence="4">
        <location>
            <begin position="80"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin fusion degradation protein UFD1 N-terminal subdomain 2" evidence="5">
        <location>
            <begin position="190"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="293"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="398"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="414"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="425"/>
            <end position="450"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF24842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP86761.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="470" mass="50596" checksum="3387B064D53F67E7" modified="2012-04-18" version="2">MDYYDDEDPMYEAEDVVDPPAQPTNRNNNARRMGDGFGGMLNSFNGIERGFGIHTDWPHLGNQMMGYGGHPSMRPPARAFSKTYKAYSTAILEIKQGRGERTGGRSNVMHGGKIIMPQDALVQLTEMDMESPFMFEIRNSSPTKRDLFTHCGVLEFIADPGTVHLPQWMMTRLELNEGDSIKLTGARLPKGKFAKVQAQSTLFLELGDHKAVLETALRNFSCLTKGDIIEIYHNMMTFEILIMELKPDDAPGVSIFETDLEVDFAAPLGYVEPTPVPRAPPATMASKLNIDTSGTQSVDARGSAAKAPGAPHPGGGSASWEAFKGGGRTMGGKVVKGKGVQKKKIEQVDSSSKIFRTDVQRIVTADTQIGDRQVPARLDLPFGTLFFGFNVKPYEPAKADGEGPGAPAAPFAKVSSSGNTLSGRSRSIAEAPEPASSSSSSSQAAPPAFSGEGHSLSQGKPKNEVIVIDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3KSA8</accession>
    <name>E3KSA8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2423 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_13402</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EFP87183.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331602.2">
        <property type="nucleotide sequence ID" value="XM_003331554.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KSA8"/>
    <dbReference type="FunCoup" id="E3KSA8">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KSA8"/>
    <dbReference type="GeneID" id="10537413"/>
    <dbReference type="KEGG" id="pgr:PGTG_13402"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13402"/>
    <dbReference type="eggNOG" id="ENOG502RDH4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_125052_0_1_1"/>
    <dbReference type="InParanoid" id="E3KSA8"/>
    <dbReference type="OrthoDB" id="4087970at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019434">
        <property type="entry name" value="DUF2423"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28219">
        <property type="entry name" value="UPF0642 PROTEIN YBL028C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28219:SF1">
        <property type="entry name" value="UPF0642 PROTEIN YBL028C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10338">
        <property type="entry name" value="YBL028C_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2423" evidence="2">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP87183.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="119" mass="13321" checksum="F4E69EC2C7D0F4C7" modified="2012-04-18" version="2">MAKSLRSKSKRAFRATKRTSTSSDYQKTEAERLQRLSKKLTGSSAEGTEQAPDQEMQIAQGAENPDDSASKPAPKVTTHGPRMSGREVWKASKRGIQLRRAPSTTVWHQRSTGKPQRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3KSE8</accession>
    <name>E3KSE8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">OTU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_12818</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178305">
        <property type="protein sequence ID" value="EFP87234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331653.1">
        <property type="nucleotide sequence ID" value="XM_003331605.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KSE8"/>
    <dbReference type="GeneID" id="10537633"/>
    <dbReference type="KEGG" id="pgr:PGTG_12818"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12818"/>
    <dbReference type="HOGENOM" id="CLU_931088_0_0_1"/>
    <dbReference type="InParanoid" id="E3KSE8"/>
    <dbReference type="OMA" id="TERIAWY"/>
    <dbReference type="OrthoDB" id="2507501at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061578">
        <property type="term" value="F:K63-linked deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22744">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OTU" evidence="2">
        <location>
            <begin position="110"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP87234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="304" mass="35032" checksum="BD29C7F27F3CDA0F" modified="2011-01-11" version="1">MGKKTSLKPAKHINEKEKELIIIEDSGDKGGNTRVVKYPTTVVDTPNSFKPPGNKGIAEDDTRGDIEVPDILEKEHYDEEVQQRANYWHEFNQDHKHWELVPKDIRAVISSIRNVNGDGHCGFRAAAVSMGQGEDSWPQIRKAMLQEMKDQPIYRNQSYLEAVADSAPYSRLEATLNYFRSPAKFNNWIKFPLHGDLLADTFQRPVIYITESMKITFLPLSHGAGPSTNPPILLLYLEGHYHYNAFKFDGEIYPAPSVSSHWFKWRQNVAKDWEKSIQLNRDEWKRRIPQQPSELPVVVDVEYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3KSJ7</accession>
    <name>E3KSJ7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12867</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178305">
        <property type="protein sequence ID" value="EFP87283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="DS178305">
        <property type="protein sequence ID" value="EHS64001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331702.1">
        <property type="nucleotide sequence ID" value="XM_003331654.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889323.1">
        <property type="nucleotide sequence ID" value="XM_003889274.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KSJ7"/>
    <dbReference type="GeneID" id="10537682"/>
    <dbReference type="KEGG" id="pgr:PGTG_12867"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12867"/>
    <dbReference type="HOGENOM" id="CLU_047292_2_0_1"/>
    <dbReference type="InParanoid" id="E3KSJ7"/>
    <dbReference type="OrthoDB" id="2504760at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="250"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS64001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="335" mass="36048" checksum="A5B535D63E530F01" modified="2011-01-11" version="1">MQTKSNNSVAFRRICHPATLHGPFDIIASLGQIGGGDTTYGQFQYDTTIGFTDPTRGEEINIMIKANCYGSAPTALQADKVYVLHGRLVARNEDAPPILFCEQDVTLNIGDSSTYMSALANKVVIEGFGIVVKREEVPGFGVGNVAQTNLHVVLRHTDYDNMSRSKVEFEILYIVPGNKILGKTFGLFRLGRETAISGYISGYIPKDKMWEVKVTAVAPTSGDQAATVVVPQRGARSGNTHRRPGLIQLGTEQNNPTPTEQPANNGSLADAANVSNYQSPAGAGPSGSGTLPVIPEYEDGEVTEAAPEPQFVNTFQKRQTSKGMIADAKKRMKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KTA3</accession>
    <name>E3KTA3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_13899</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178307">
        <property type="protein sequence ID" value="EFP87528.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331947.2">
        <property type="nucleotide sequence ID" value="XM_003331899.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KTA3"/>
    <dbReference type="GeneID" id="10538427"/>
    <dbReference type="KEGG" id="pgr:PGTG_13899"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13899"/>
    <dbReference type="HOGENOM" id="CLU_2074305_0_0_1"/>
    <dbReference type="InParanoid" id="E3KTA3"/>
    <dbReference type="OrthoDB" id="10416911at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP87528.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="118" mass="13692" checksum="65ABF9AF2194F83B" modified="2012-04-18" version="2">MGLFNSIRWQRLTWYPNTRLYPTVPFDKATDKPTRHPTPAEVQKACKFADRNFYLMNAGRPVLLDPRNPESVIAIMDFTPWNRLTETDKANLEFVSTFLHQTKEFVNPVSSSTRSWGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KU94</accession>
    <name>E3KU94_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14584</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178309">
        <property type="protein sequence ID" value="EFP87869.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332288.2">
        <property type="nucleotide sequence ID" value="XM_003332240.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KU94"/>
    <dbReference type="GeneID" id="10534848"/>
    <dbReference type="KEGG" id="pgr:PGTG_14584"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14584"/>
    <dbReference type="HOGENOM" id="CLU_101479_0_0_1"/>
    <dbReference type="InParanoid" id="E3KU94"/>
    <dbReference type="OrthoDB" id="2507549at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172209" evidence="1">
        <location>
            <begin position="25"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87869.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="188" mass="21390" checksum="F31B700E6BA27B6E" modified="2012-04-18" version="2">MKASQHGCRLTLFILFSILQLNRAASSWNPLDLFRGSRAWSSAGEASSAEIALPRQKIMAKSNVNWIKLPDSELSLLSIRYRKEDEAWTVTNETGFPITFSMRKFDVEEMLAKHPRVDYDDEMETVEDTHGKVGAKEEVEGEEGVEGQKKVYRQLTVQPKEVLVEHLPKNVDALLVNIESWDRSAVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVR2</accession>
    <name>E3KVR2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">OTU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_14486</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178313">
        <property type="protein sequence ID" value="EFP88402.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332821.2">
        <property type="nucleotide sequence ID" value="XM_003332773.2"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KVR2"/>
    <dbReference type="GeneID" id="10535875"/>
    <dbReference type="KEGG" id="pgr:PGTG_14486"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14486"/>
    <dbReference type="eggNOG" id="KOG2605">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_261231_0_0_1"/>
    <dbReference type="InParanoid" id="E3KVR2"/>
    <dbReference type="OrthoDB" id="2564822at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22744">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.80:FF:000047">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34863">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34863:SF1">
        <property type="entry name" value="OTU DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OTU" evidence="2">
        <location>
            <begin position="650"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="497"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="562"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="817"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1157"/>
            <end position="1211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="513"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="527"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="562"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="590"/>
            <end position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP88402.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1304" mass="147682" checksum="C1125356A7C3B355" modified="2012-04-18" version="2">MYNLPIPDEEDYDLDSWAISIETHQLSTILGTMGLVTLAHHSLEIQDHQDFADYVFVAQGGCTIRIPPKFKAGVLKKLWKNYHIIAHPSKNPLVLNMGSPSCTVGMLQEILHQLCVPTLRLASYGMKCPFSLVFYNLGDGVRGFMKTEQKKNHFDIGFIHYPYYGTKHFRHEILLAKRPPGSRKCQSESYPTLLELGEGFGTINLKPDHEAITRLKCYSSLTHALKATSQKDMKYAPTMATTWNTRLDILEKKVDVLAANDPVHLCGLRFEATVYASTVDEAQEIVRSGYLHPLAYSHGPDHPNEQIALDFKAIPVLEYFAYVALMLKKLKQMKTEVSQKHETQRKITDIQKQAMVDVANAIGWNGGRGQRPTSVDRKKVPEPWWDNYGDSWEERRKKKGDVDPEELLKRSPLDLWQLRPRRVFPCAICNAPDNPNPPPLKKSALYPPGSITSIAGKRNIILKCNHCSMKTDSQKKVVIMLATWIEKLGLPLLSEDKRRTLPGGFSKDNPEKIPSPSPPPQTIPEESSDKSSPEPQRKKDQLIPVVVVPKIAMKKNLVETKTIPSKTRKNNSLADSVKTNIDLASQARTTTKEKLKTPQDRSHGRTLLGWTGFADFLHKNNLCNDSSSHIPSSEKIPTSSKKDILDGWKLSENFIKGDGNCLFRVVATWLLGHQDQHQKIRTPCYDELAKNQDHYAPFVTRDDLRQPGQTAQEGLLIQSLPEEEIYNFFLRKSQSDGFWGGDHHLSAVSHVFGIQLVVLSQNGGTYTFQPRTGELKRTIYLWYNGNNHYEIIWNPQLHSAQSANPVFQTPQRNTLPGKSVLKPSSFPRNPADKSSTTILFNPQAKNNKRVSFGTLVHEKIITTGNSLLHIDPRLQTPPSQLTKCSSTISNIRKSQHFNSAPPQPRNPVVVIPPISFFPWNQPLPPNLKRKRNRIAGLEKNHGLPIIDGYWNRVNERLKLETGKNKKTIPYGVIRFSMPEGLLEGWYISHNLIQTSKSIMDPDITGFYHAVNYWMSGTQDRHSIFQKDLAEHLGKQLPQMSKNSSRLVRVDNKKPFSVDEIKKAFHLVSDGKKPDQVIYSTAATMLRVVLVVVEMGSTSATWSQYSPFGSHNLAPFPSIYLFFNLNVNRFQIMWAPQIHQPVVCPENPELETRLLGNTNLLSNPQSPPSHDHTFSVQPSKNKTVADPNPDSLPPQKRFKTQPTSQIPHHTLEPNCMPTIEDLRMVDFGPALTRLHIYHHKDACNRKCRSISQAAYFELGDFRYVGWRLSIGFFPDDQNSIWRVLSLQPSGIPFLRDSTNIEDSIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3KWT8</accession>
    <name>E3KWT8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Vacuolar import/degradation Vid27 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_14721</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178316">
        <property type="protein sequence ID" value="EFP88755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333174.1">
        <property type="nucleotide sequence ID" value="XM_003333126.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KWT8"/>
    <dbReference type="GeneID" id="10541706"/>
    <dbReference type="KEGG" id="pgr:PGTG_14721"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14721"/>
    <dbReference type="eggNOG" id="KOG2395">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_561554_0_0_1"/>
    <dbReference type="InParanoid" id="E3KWT8"/>
    <dbReference type="OMA" id="EPENDHY"/>
    <dbReference type="OrthoDB" id="10251113at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002645">
        <property type="entry name" value="VID27-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040458">
        <property type="entry name" value="Vid27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013863">
        <property type="entry name" value="VID27_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31913">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31913:SF0">
        <property type="entry name" value="VACUOLAR IMPORT AND DEGRADATION PROTEIN 27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08553">
        <property type="entry name" value="VID27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Vacuolar import/degradation Vid27 C-terminal" evidence="2">
        <location>
            <begin position="240"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="211"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="227"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP88755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="486" mass="53242" checksum="8FC8531913C537E3" modified="2011-01-11" version="1">MGTYGPGPSGFSSPSESDDESTTRDDDIENLRAEKDGKIAEKDNQIAELLHKVKQLEFEAPNKSQASLAPADGQLNLPAAAISGGSLTIAANQNQTFLNRPNPFLSSPANPFTNSSARSHQQSGVASETRYGPSQLLVERSSTTTTTSVRIASTGGAFSGSSSNGITHATIEYRAESSFTRWSGLKNSTMVPERHQDKSDRHFDNNVQVEVIEEEEGSSSDDEEDYEPRVSSRSRRNEPTNDHYAMGYKGVSCVVGGNTVKTFDDNSNGSGKLKLLAKIPGLKTPDGKRDLFPSKIMLHREDSNLIIQDKRSANSLYNFDLEVGKVVEEFKLGRQGAPLEDFCPSLKFGQMTSETTFVGVSSDTLQTIDPRLNGYKAVTHHDYKTNPKFSCVVTTEFGWIAVGSHKGDIRLYDSITSYRAKTWFRGSHGPVLALDVSKNGAFLIATYQTYLVLYESGMGFIKSIANSPKGSGTRLQLTREHQMFIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3KXQ4</accession>
    <name>E3KXQ4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Small subunit ribosomal protein S19</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_14957</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178319">
        <property type="protein sequence ID" value="EFP89116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333535.1">
        <property type="nucleotide sequence ID" value="XM_003333487.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KXQ4"/>
    <dbReference type="STRING" id="418459.E3KXQ4"/>
    <dbReference type="GeneID" id="10546666"/>
    <dbReference type="KEGG" id="pgr:PGTG_14957"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14957"/>
    <dbReference type="eggNOG" id="KOG0118">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_28_1_1"/>
    <dbReference type="InParanoid" id="E3KXQ4"/>
    <dbReference type="OMA" id="GWEDRSY"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd21608">
        <property type="entry name" value="RRM2_NsCP33_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048289">
        <property type="entry name" value="RRM2_NsCP33-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052462">
        <property type="entry name" value="SLIRP/GR-RBP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48027">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN 87F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="32"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="108"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="164"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="171"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP89116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="206" mass="20500" checksum="785E63E71440D9F7" modified="2011-01-11" version="1">MLRALFKPSSRVLSSILHPRTLSSMSSPPGGNKLFVGNLAWTTDSGSLKSAFGQFGDVVDAIVMQDRETGRSRGFGFITFKEEAAAPGAIEALNGQELDGREIRVNYANSQTGGGGGGGGGQRQGGGGGYRGGYGQSRGGGGGGGGYNSSYGGGGYGGDQGGYGNSTQQSYGGGGGGYGNGGQGQGNWRGDQQNSYNAPSGGGGAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KY22</accession>
    <name>E3KY22_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15064</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178320">
        <property type="protein sequence ID" value="EFP89223.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333642.2">
        <property type="nucleotide sequence ID" value="XM_003333594.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KY22"/>
    <dbReference type="GeneID" id="10546860"/>
    <dbReference type="KEGG" id="pgr:PGTG_15064"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15064"/>
    <dbReference type="HOGENOM" id="CLU_1230421_0_0_1"/>
    <dbReference type="InParanoid" id="E3KY22"/>
    <dbReference type="OrthoDB" id="10277037at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89223.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="210" mass="23343" checksum="B870920153B1B4BF" modified="2012-04-18" version="2">MVPYSSSASLQTHARLLIKQNVGDPQLVSEASETGRKAMHPIEPVPHPVGAEPGSRKLSKRFLKCFCGYPAVDGATGARSVEATASKSRLPEVESTKISPGSSYKGRPRELIPKSGGRSRSEKERPGSVDLDPEKEDESMDGQDVNFRFVIDSLTPEQEQAAINWVQSQNKSRNLDRQNGFFYPPRYQVYGKHVDAQFPPGYQEGYARYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KY46</accession>
    <name>E3KY46_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15088</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178320">
        <property type="protein sequence ID" value="EFP89247.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333666.2">
        <property type="nucleotide sequence ID" value="XM_003333618.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KY46"/>
    <dbReference type="GeneID" id="10546884"/>
    <dbReference type="KEGG" id="pgr:PGTG_15088"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15088"/>
    <dbReference type="HOGENOM" id="CLU_468625_0_0_1"/>
    <dbReference type="InParanoid" id="E3KY46"/>
    <dbReference type="OrthoDB" id="2510287at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="529"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89247.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="582" mass="68217" checksum="A41EDFABBD2361D8" modified="2012-04-18" version="2">MAKTTAKLKKTFQTLEALDRISEDIHKKILLQEDNFSIYEIAFTDNIVKELKERKISKIDLNSWKSIAKEKTGRIRSGILKSLTSAERQSKQETWWSRLVDFFKYIFRKSKWKKEQLSLKLRKLRSNSMASHNESSDEERTAIYRLSIKDYNGFTFTNREVQLIERMAGGRSHLTSTDHDLITEISRCSMTGTQRKLLELKVYFSQIAREYPNTINSDIVKALQELEEKMKRLKKWSSGEVKLYNRLGTMKDIKAIRPHLGNRKRWILQRVIIQWKRSERMRFYMKAFVILGIKPEELFSKAEEAVATKLRRWYELYSEDFYSGSIGCPFDFSEEQWDIVRRYVEEYDSMLDSGRQLFKNAQLIDTAKSHVQGYQDSTVEKTPLHPIFRYSINGLKKLEELGAQLSVEENLLLKGLKSKRNPSLSQKELEKLAVEGIILETGGVPTYLQESVIVLDSTNTKHLSPLTTTDQKIIGILKGLGEDELEELKRLGSLSRNDPEFKSETKFIRMLRILIEVDIRSNFNVDKTRKLLQRLIQLKKNEIQRARSQFEKREQDMNDFQSIEALPGLEKVLYKFEDVSRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KY55</accession>
    <name>E3KY55_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_15097</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178320">
        <property type="protein sequence ID" value="EFP89256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333675.1">
        <property type="nucleotide sequence ID" value="XM_003333627.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KY55"/>
    <dbReference type="GeneID" id="10546893"/>
    <dbReference type="KEGG" id="pgr:PGTG_15097"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15097"/>
    <dbReference type="HOGENOM" id="CLU_070872_0_0_1"/>
    <dbReference type="InParanoid" id="E3KY55"/>
    <dbReference type="OrthoDB" id="10305548at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP89256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="262" mass="30143" checksum="2CCAD37B62E143AB" modified="2011-01-11" version="1">MSHSNSFFFGDASHKSYSKSSLKEVVRNKNDPSELTLDQLDKKGWRFPKGLAHQSQFDKMPVLMDLKKALQAMREFHRRGAHTEGSTVSSSIKGNKLKGNWDHLDHDMGKLEIWENAHKDKRTRFESGTTELIPPYKITLEETWDYIYKALQVTHFLPQDEKGHLFFANLQILLADALEIGTKQEQKTSFAQRFAANLRPLLAQPQFPRLLSEASGREILRPMDRLSKWLKQNAELEFSNCEEHTTHTDKVPQAIPSVLVQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KYN1</accession>
    <name>E3KYN1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15269</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178322">
        <property type="protein sequence ID" value="EFP89427.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333846.2">
        <property type="nucleotide sequence ID" value="XM_003333798.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KYN1"/>
    <dbReference type="GeneID" id="10547314"/>
    <dbReference type="KEGG" id="pgr:PGTG_15269"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15269"/>
    <dbReference type="HOGENOM" id="CLU_1180709_0_0_1"/>
    <dbReference type="InParanoid" id="E3KYN1"/>
    <dbReference type="OrthoDB" id="10320986at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89427.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="235" mass="26290" checksum="7DABBB841F3FA96F" modified="2012-04-18" version="2">MDPDQFFLHTHQSPSKKSLSIPEDTEENFKHILYSVTQNVWVPKSFNPISCSSLPPLDGPVYLNVAIELIGKLEDPQETIIESIIQTILTTLYQFSKGTTVCLTGITPEETQFGNATFALSFEPIQEPLKVLLAGQNIGRLSSLQLDVLFEARRHSHPLSFTESMKQLTFNLICDLMNHLATEIPLVFNSKWRKSLPNKLATIIYMSTYTASIADSAKDEEARSEVGKMPVVSES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>E3L060</accession>
    <name>E3L060_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">O-acyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGTG_16235</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Sterol O-acyltransferase that catalyzes the formation of stery esters.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Endoplasmic reticulum membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178327">
        <property type="protein sequence ID" value="EFP89947.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334366.2">
        <property type="nucleotide sequence ID" value="XM_003334318.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L060"/>
    <dbReference type="FunCoup" id="E3L060">
        <property type="interactions" value="164"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L060"/>
    <dbReference type="GeneID" id="10545762"/>
    <dbReference type="KEGG" id="pgr:PGTG_16235"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16235"/>
    <dbReference type="eggNOG" id="KOG0380">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018190_2_1_1"/>
    <dbReference type="InParanoid" id="E3L060"/>
    <dbReference type="OrthoDB" id="10039049at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034737">
        <property type="term" value="F:ergosterol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008204">
        <property type="term" value="P:ergosterol metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004299">
        <property type="entry name" value="MBOAT_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014371">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10408:SF9">
        <property type="entry name" value="STEROL O-ACYLTRANSFERASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03062">
        <property type="entry name" value="MBOAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000439">
        <property type="entry name" value="Oat_ACAT_DAG_ARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="115"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="151"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="354"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="409"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="540"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="256"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="200"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="260"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="499"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR000439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000439-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EFP89947.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="562" mass="65116" checksum="F4C5612F8FD84CE8" modified="2012-04-18" version="2">MTSLIQPEPQATNTKENQTTVKTTTNQITTVETKTTVAILKTNPKQQQQQQQLLVNFTPRLSTLDPHNQLLHQNPLRGLYALFWILLGFHIIATLHASGSLVIQSPLARLFSKHLPQLALVDLCLVASTFSALGFAKLLSNRYFDYCHTGLILQHLFQFSWLGAWILYIFYREWPWIQSGFLTLHSITMLMKIHSYCATNGEYSVQLKSLESKMKELKRSKVIEIKEDDQQHHKTLVSTVVKTGIYQKLQQFVDQKKEQPTTIDQSLGSNSQSDQGPEKPMNDEMMEQQVHETIDLLDQLGARNLRDSHDDDDQKEDQYSGQPWPENLTISNFTDYLLIPTLVYRLRYPRTKEIRIFFVVEKVFAVVGIFGLIYMITQYYIWDQVTNIKAFDRDHRSSALHLVDNVIRLVVPFTINYLLIFYIIFECICNAFAELTRFADREFYSDWWNSCSFDEFSRKWNKPVHHFLLKHVYASTISSYGVSRSAAAIMTLFLSSLVHELLMVIVTRKLRLYLFLAQMTQLPLTYIGRSKLFKTRPALANAFFWVGLISGFPLLAVWLVSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3L0D0</accession>
    <name>E3L0D0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15876</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178328">
        <property type="protein sequence ID" value="EFP90028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334447.1">
        <property type="nucleotide sequence ID" value="XM_003334399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L0D0"/>
    <dbReference type="GeneID" id="10545925"/>
    <dbReference type="KEGG" id="pgr:PGTG_15876"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15876"/>
    <dbReference type="HOGENOM" id="CLU_845046_0_0_1"/>
    <dbReference type="InParanoid" id="E3L0D0"/>
    <dbReference type="OrthoDB" id="10274318at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="329" mass="37533" checksum="31CCBE2EB62FC044" modified="2011-01-11" version="1">MDSSTPTGLHSPPEVETAPMKDTISQPAVDHSCFTPTMFLRPPGSPAARDLNIDSPSEQGVCRKRKDPEDNTKADSTASSMKTPERLPKLPRTETLIAWNHDLLLDLVTFFRMWQFHHESLTQTARPGDLIPTYVSEYQAIRAKFPHLAGICLTELTQKYKELVVRWTGITIKLETSLEDAHRGIRARIFQQHMSPSVFFLLKDAIERPLSLDLSGLVLPEKDFKPPTDVADKAGALVSISRAHHTARLTLNSLKTSREEREFEMEREKQDVIYSTLMRYMDGDQARMKRRHETISTNKANEEDRLTDAYISLYHGYRASEAEAPCYRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3L103</accession>
    <name>E3L103_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16506</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178330">
        <property type="protein sequence ID" value="EFP90228.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334647.2">
        <property type="nucleotide sequence ID" value="XM_003334599.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L103"/>
    <dbReference type="GeneID" id="10546370"/>
    <dbReference type="KEGG" id="pgr:PGTG_16506"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16506"/>
    <dbReference type="HOGENOM" id="CLU_035448_0_0_1"/>
    <dbReference type="InParanoid" id="E3L103"/>
    <dbReference type="OrthoDB" id="2498534at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="414"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="512"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="122"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="423"/>
            <end position="439"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90228.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="658" mass="74260" checksum="E2BA17083F6D698A" modified="2012-04-18" version="2">MLSAQFSCSCPACSWPVGPIPAGFTTHQAIHPSHPSRFRHLQPIHYHQPIHSFQHQEMNFDLDVLDPALDAAQTHDSTPASLQQSPYNGDLFLEDDEDDLSQSGVDSSKNTPAVSGRQASGRGRKKAKATKKSISEPTLHISLDYCLYVNVHNPHISVKQRAASTKSDWDKIVPSKDDLPYLNTNLMHRQWDELRDEILRIIGNSQRNLDVFLDQVRKTDNLKFHLYISNSHFFPFRRAYYCSSDEELRPFAEEVAKKPLQKVFIRVIMDDPSAQEKSHSAKDKSDKQKKKVEKILAMAVGSDKERVPLQREQARQLQNTRSDVSGDPIGPWVVRLRQHLEQKSNAKPSEVIFWPHKDDPTLVLRVNHNRLWAWANVLEAKSAGHITDANRHVDMDTPPNGSTFQWERRSGISPLKHRAAPGNSHNNNPRRLSVGNQGRTDALPRQFSGCSVADVNRMSPVSRCDTLNSDAAVEGREEALDLRNIGISNVVDLVSKSSNDSIDYLNDVSVDSSRSVNHLDQSSRNSGRPADAEHPRKMARSPSAYPTCDYRCISVHPRDTSPSRSPPRKSRSEPRLPLLPAGKNMTMNDFLRHCNIEVDDPMCQILIKGHDVKHWSFFRGKSDEQLMRIGFTRGLATHLSDGASELELTMVQQEITEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>E3L1I6</accession>
    <name>E3L1I6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_16171</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178332">
        <property type="protein sequence ID" value="EFP90411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334830.1">
        <property type="nucleotide sequence ID" value="XM_003334782.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L1I6"/>
    <dbReference type="STRING" id="418459.E3L1I6"/>
    <dbReference type="GeneID" id="10530305"/>
    <dbReference type="KEGG" id="pgr:PGTG_16171"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16171"/>
    <dbReference type="HOGENOM" id="CLU_055324_2_0_1"/>
    <dbReference type="InParanoid" id="E3L1I6"/>
    <dbReference type="OMA" id="QDGMETI"/>
    <dbReference type="OrthoDB" id="76487at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023">
        <property type="match status" value="1"/>
    </dbReference>
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    </feature>
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        <source>
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        </source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="97" mass="10750" checksum="9275FDF78BEFBC12" modified="2011-01-11" version="1">MHPWLKTKAAILLTSCIAFTKQLDCPRRRCNGVSNSTGSNYTEPPQCHPCTQLVPLEYHECTLCGHQWTAPMLNRCPKANTNHANQQCARPNTSAAA</sequence>
</entry>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16804"/>
    <dbReference type="HOGENOM" id="CLU_1518589_0_0_1"/>
    <dbReference type="InParanoid" id="E3L2M7"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="166"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90778.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="177" mass="19400" checksum="8C52F0DBDF4C870E" modified="2012-04-18" version="2">MLSAKRVEGAGPKAKPIGEYSSSADPEGEAQQTAIGREEAVGMVMIKAERPIKKRRSPSSVGHHQPEQLSESSYGGHWRQSSEDSSVPSVVELKSQYPLNPSGDHSRSLRRSGLSALPTNPTNNPFDDFEILDNKQLPEERNQTLLSSGEDKIDGLQDSSNGYFRPPDKADHHDCFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3L2Y8</accession>
    <name>E3L2Y8_PUCGT</name>
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        <recommendedName>
            <fullName evidence="14">Cation/H+ exchanger transmembrane domain-containing protein</fullName>
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    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Puccinia</taxon>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fungal Na(+)/H(+) exchanger family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178339">
        <property type="protein sequence ID" value="EFP90913.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335332.2">
        <property type="nucleotide sequence ID" value="XM_003335284.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L2Y8"/>
    <dbReference type="GeneID" id="10528326"/>
    <dbReference type="KEGG" id="pgr:PGTG_17185"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17185"/>
    <dbReference type="eggNOG" id="KOG4505">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_314519_0_0_1"/>
    <dbReference type="InParanoid" id="E3L2Y8"/>
    <dbReference type="OrthoDB" id="2500422at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015385">
        <property type="term" value="F:sodium:proton antiporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030007">
        <property type="term" value="P:intracellular potassium ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120029">
        <property type="term" value="P:proton export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042391">
        <property type="term" value="P:regulation of membrane potential"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036376">
        <property type="term" value="P:sodium ion export across plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035725">
        <property type="term" value="P:sodium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004712">
        <property type="entry name" value="Na+/H+_antiporter_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31382">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31382:SF4">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0050">Antiport</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0732">Signal</keyword>
    <keyword evidence="7" id="KW-0915">Sodium</keyword>
    <keyword evidence="10" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173042" description="Cation/H+ exchanger transmembrane domain-containing protein" evidence="13">
        <location>
            <begin position="28"/>
            <end position="984"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="197"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="224"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="254"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="295"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="326"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="416"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="444"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="484"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="525"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="14">
        <location>
            <begin position="149"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="596"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="694"/>
            <end position="769"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="789"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="704"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="724"/>
            <end position="735"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="11">
        <location>
            <begin position="745"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="757"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="789"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="821"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="845"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="866"/>
            <end position="883"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EFP90913.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="984" mass="108166" checksum="63445FDCA88C7071" modified="2012-04-18" version="2">MSPSTTRLKYWNAIIFFTLSLKPLVSISSPTSSLKGDQALPLTWLDLEIEPPFFKVDQQSFQALDYDFVSRDASAIIQNLRIDTAGRHLRKRDVDPSIVYNNSINAGVPQPLSKATKKLFKIYPDVRTVYIAMALIPAFIICYGTLSTFIRSKLHLGEAMLAVTFGILLGPYVAGIFDPRSWGGEASFDEVTLEITRIIVALDVFSVGVELPAAYILHHWRTMICLLGPVMLAGWFITGAFILIFVPALSYLEALAIAACICPTDVLLASSVVGKGKYAKNHVPAHLRHMLQAEAGANDGIAILLLYLTLFILLRKDYSVAHAIGSWVTITALYHIMVGIVMGVVAGILVREGLKFSEKHELIDTESMVPIYISLALLTAGVCVLAGTDDIVAAFACGTAFGWDGYFSEEVEASNFSAIISNLVNTFAFIYVGATIPFRAWNDAVLTLVAWKMFLLVLSILLLRRLPIMFLLRKLIPELRTNKEALFCAHFGPIGVSGLFISFLATKKLPTPNVPAESSLDILSLTIQPILYLLIFCSTFIHGLSIPFFTITKSPVSVAIEAAHLSDQVYLSNEEVCPCMKAVTLTPYLQPMSMHQQLGNRASPTSQPELESQFPGNQPNQSSNEVIITHATLNPIMCEDEATNKGQPLMPTKVEKTFLSPSDSHRAIQLTGSSPRNSVSWLDFSSEKVELSGLEGSTSKRTSAIREAEARDGGEDDMCLTEGCSREGVERDSQHSRGPVMMGEWESDQWEGEEVENNGSPRTTPPQATHELHACLPCITRPRSFSGRITRDLKHESRRISRVSNGNTIQEAIAERRRRSVSSSDLSSSPSLHCSPKFDSDGSPGKSNISGSSPGRSDTNELKSKVQGQWQSEPGGTVNPFQHQFVFTPNENSVDTDEIKAWPAGGSSIYPSPTPWQNLTKNSGSNAEVAVQSNYLDPTYYRMPGPSTGETVNVPLVRRESYSSSFEAAIHRDCQNYKKRYYAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3Z9</accession>
    <name>E3L3Z9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_17131</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178344">
        <property type="protein sequence ID" value="EFP91274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335693.1">
        <property type="nucleotide sequence ID" value="XM_003335645.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L3Z9"/>
    <dbReference type="GeneID" id="10529020"/>
    <dbReference type="KEGG" id="pgr:PGTG_17131"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17131"/>
    <dbReference type="HOGENOM" id="CLU_084549_0_0_1"/>
    <dbReference type="InParanoid" id="E3L3Z9"/>
    <dbReference type="OMA" id="IGNIGCR"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.20">
        <property type="entry name" value="Insect antifreeze protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173090" evidence="2">
        <location>
            <begin position="19"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="104"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="104"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="130"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP91274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="200" mass="20951" checksum="6F6DD67C1205238F" modified="2011-01-11" version="1">MQFTVLAAALAVFTGVLASPVVETAEAPAAPKAAETAEAAQTWPLYRGYGGYGGINANVGFNANAGFNNGYNGYNGQHGNGQFSNRQFGNGQFGNGQFGNSQFGNGQFGNSQFGNSQFGNGQFGNGQFGNGQFSNGQFGNSQFGNGQLGNGQFSNGQFGNGQFGNGQFGSNGWGRYGGNSGYSDYNSQQRQGYSNYYNNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>E3L499</accession>
    <name>E3L499_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent DNA helicase II subunit 2</fullName>
            <ecNumber evidence="4">3.6.4.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">ATP-dependent DNA helicase II subunit Ku80</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PGTG_17028</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
            <location evidence="2">Telomere</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ku80 family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="4"/>
    <dbReference type="EMBL" id="DS178345">
        <property type="protein sequence ID" value="EFP91374.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335793.2">
        <property type="nucleotide sequence ID" value="XM_003335745.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L499"/>
    <dbReference type="FunCoup" id="E3L499">
        <property type="interactions" value="451"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L499"/>
    <dbReference type="GeneID" id="10529287"/>
    <dbReference type="KEGG" id="pgr:PGTG_17028"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17028"/>
    <dbReference type="eggNOG" id="KOG2326">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010975_1_1_1"/>
    <dbReference type="InParanoid" id="E3L499"/>
    <dbReference type="OrthoDB" id="30826at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043564">
        <property type="term" value="C:Ku70:Ku80 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042162">
        <property type="term" value="F:telomeric DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00873">
        <property type="entry name" value="KU80"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.410:FF:000073">
        <property type="entry name" value="ATP-dependent DNA helicase II subunit 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1600.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.290.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006164">
        <property type="entry name" value="DNA_bd_Ku70/Ku80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024193">
        <property type="entry name" value="Ku80"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005161">
        <property type="entry name" value="Ku_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016194">
        <property type="entry name" value="SPOC-like_C_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604">
        <property type="entry name" value="KU AUTOANTIGEN DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12604:SF4">
        <property type="entry name" value="X-RAY REPAIR CROSS-COMPLEMENTING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02735">
        <property type="entry name" value="Ku"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03731">
        <property type="entry name" value="Ku_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00559">
        <property type="entry name" value="Ku78"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100939">
        <property type="entry name" value="SPOC domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="8" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="15" id="KW-0234">DNA repair</keyword>
    <keyword evidence="13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-0347">Helicase</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-0539">Nucleus</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0779">Telomere</keyword>
    <feature type="domain" description="Ku" evidence="19">
        <location>
            <begin position="374"/>
            <end position="512"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="340"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="352"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00031847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="SMART" id="SM00559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="EFP91374.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="568" mass="62857" checksum="0A865D42DBD48B7C" modified="2012-04-18" version="2">MAGGRELTIYAIDVSPSMSEKRTADDPSTGATTSVTNLEYGLEVVKHQVANMLLAGLKTVHCGVVLFGTERTDNSLASSGYEYIWEYVRPGQPSAITLLHINKIEQHLTTSEKPFKGDLLSAIILCNHHMGETLAAKKWTRKIVLVTDAQTETDWSGWKDQRDKMRADNVTLEVVGLDFDDPEVGYIQPNKPEIKATNEKILHKLCRSLANASSCSTALVAVMNAQSPQPRVVGSTPAPVLLSLGYPDQETAAAEGYDPQESLFMRCELRKCTSIIRPMSAKKMSKLGISSELDRRRKDLDAMDAGAEMQQYLPADKDNQDTDGILGLLQVNRKHVYAAKKSKDPPAKAAGPDDDSDDLGLEDSSDEEPGLLEAREVDRENLAKAYKYGASLVVVDKEDEAAIRQSFSECLEIRGFVNLKNIPRHHLLNNVYYLYPIATDYGSQISFSAIVRAMNDANRAALARYVGRSVVAEPKLVILIPVVEPTIRYFLFIQVPFVDDLRQYAFPPLPTGPILGNRTVRTQYVPTDEMQDAMDELVDKMDLSAEEGSKTAELSVIFRLAHAWSMSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>E3L519</accession>
    <name>E3L519_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Cytochrome b-c1 complex subunit Rieske, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGTG_17500</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Rieske iron-sulfur protein family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178350">
        <property type="protein sequence ID" value="EFP91644.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336063.2">
        <property type="nucleotide sequence ID" value="XM_003336015.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L519"/>
    <dbReference type="SMR" id="E3L519"/>
    <dbReference type="FunCoup" id="E3L519">
        <property type="interactions" value="266"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L519"/>
    <dbReference type="GeneID" id="10531582"/>
    <dbReference type="KEGG" id="pgr:PGTG_17500"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17500"/>
    <dbReference type="eggNOG" id="KOG1671">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055690_0_0_1"/>
    <dbReference type="InParanoid" id="E3L519"/>
    <dbReference type="OrthoDB" id="1637982at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045275">
        <property type="term" value="C:respiratory chain complex III"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008121">
        <property type="term" value="F:quinol-cytochrome-c reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006122">
        <property type="term" value="P:mitochondrial electron transport, ubiquinol to cytochrome c"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03470">
        <property type="entry name" value="Rieske_cytochrome_bc1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cytochrome b-c1 complex subunit Rieske, mitochondrial"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Ubiquinol cytochrome reductase, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037008">
        <property type="entry name" value="bc1_Rieske_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014349">
        <property type="entry name" value="Rieske_Fe-S_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005805">
        <property type="entry name" value="Rieske_Fe-S_prot_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004192">
        <property type="entry name" value="Rieske_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006317">
        <property type="entry name" value="Ubiquinol_cyt_c_Rdtase_Fe-S-su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01416">
        <property type="entry name" value="Rieske_proteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10134">
        <property type="entry name" value="CYTOCHROME B-C1 COMPLEX SUBUNIT RIESKE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02921">
        <property type="entry name" value="UCR_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00162">
        <property type="entry name" value="RIESKE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81502">
        <property type="entry name" value="ISP transmembrane anchor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Rieske" evidence="14">
        <location>
            <begin position="330"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="24"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="64"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="199"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00072517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EFP91644.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="401" mass="43527" checksum="2DBE6CE25D265232" modified="2012-04-18" version="2">MQKQKLKGLKILWKFTFNLIPKPDRVPGIGSPGRSAWGRGRSRCTGPGEARAQENRTHLTGHRTQPQETTQPTTSSDSTSKANNYTNQAKLSKMVLNNLTANWASLSRKSFTLPTTVAACPKGIPIAPRVNLAARSAHDAHGEHHDEAHGPSSIKDRHNFRIPSFAARVNVPTCSIAQLSSRTPINANPTLSHHRQFGTSTAKNSMIPKSSGSASFQDTREVPNYSSYKTVSEDSGRVFSYLMVGSMGLVTASGAKSLVSDFLTNLSASADVLALAKVEIDLANIPEGKNVIIKWRGKPVFIRHRTPAEIDEANSVDISSLRDPQADSDRAKRPEWLVMLGVCTHLGCVPIGEAGDFGGWYCPCHGSHYDISGRARRGPAPLNLEVPAYDFNDEEGKLIIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>E3L5X9</accession>
    <name>E3L5X9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_18405</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178355">
        <property type="protein sequence ID" value="EFP91954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336373.1">
        <property type="nucleotide sequence ID" value="XM_003336325.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L5X9"/>
    <dbReference type="GeneID" id="10531906"/>
    <dbReference type="KEGG" id="pgr:PGTG_18405"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18405"/>
    <dbReference type="HOGENOM" id="CLU_2027895_0_0_1"/>
    <dbReference type="InParanoid" id="E3L5X9"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP91954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="122" mass="14125" checksum="DAA3C612748A784C" modified="2011-01-11" version="1">MASRFGLLEKQFYKQSFNKFRAQESESLIELVEDFPSKSTFWVLIENWEGPRSDGKLSSKVSSTQFPSGPILPTPIETLWTTVNGHFYHGWFISNCQDKWGTRLSERHLRAVGAYNFERSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3L6P8</accession>
    <name>E3L6P8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_18120</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178360">
        <property type="protein sequence ID" value="EFP92223.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336642.2">
        <property type="nucleotide sequence ID" value="XM_003336594.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L6P8"/>
    <dbReference type="GeneID" id="10537716"/>
    <dbReference type="KEGG" id="pgr:PGTG_18120"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18120"/>
    <dbReference type="HOGENOM" id="CLU_025212_0_0_1"/>
    <dbReference type="InParanoid" id="E3L6P8"/>
    <dbReference type="OrthoDB" id="2499072at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033018">
        <property type="term" value="C:sarcoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051279">
        <property type="term" value="P:regulation of release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10033">
        <property type="entry name" value="CALSEQUESTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10033:SF0">
        <property type="entry name" value="CALSEQUESTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP92223.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="328" mass="36768" checksum="DC7B46C881279611" modified="2012-04-18" version="2">MLALIGNVAEKTVTKFLGENKPPRRKSGWNQFAAFSLESLKYPVPPKGASKGWEERNIEIGKAWKLLDVDERAVFSARIFQHFSGIPCDFEDEAEDDEEESDLTTDEIELYKPLYDKLVNIEKVNIVAAKGSECQGENTSQTYKKALSATLKLNSELYTISNVYNTTYYLLTATRSPGKNSFCKEYSNDAAWLALAKKKWSAKETFEAYSHAREIQGDLEKIVGGSAGQKQQKDSDLVKIKLRQALNDLLTDALGGDVEAVFPKTENPAGSLAVSHPELKLVQLAHSKLSQESLKKGFEKILTGDRRRWLADIKSGHFKIEHTETEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3L6U4</accession>
    <name>E3L6U4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Large subunit ribosomal protein L31e</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_18484</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL31 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178361">
        <property type="protein sequence ID" value="EFP92269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336688.1">
        <property type="nucleotide sequence ID" value="XM_003336640.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L6U4"/>
    <dbReference type="FunCoup" id="E3L6U4">
        <property type="interactions" value="361"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L6U4"/>
    <dbReference type="GeneID" id="10537763"/>
    <dbReference type="KEGG" id="pgr:PGTG_18484"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18484"/>
    <dbReference type="eggNOG" id="KOG0893">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112570_1_1_1"/>
    <dbReference type="InParanoid" id="E3L6U4"/>
    <dbReference type="OMA" id="EVWKQGI"/>
    <dbReference type="OrthoDB" id="9739313at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00463">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.440.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000054">
        <property type="entry name" value="Ribosomal_eL31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023621">
        <property type="entry name" value="Ribosomal_eL31_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10956">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10956:SF0">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01198">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01380">
        <property type="entry name" value="Ribosomal_L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54575">
        <property type="entry name" value="Ribosomal protein L31e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP92269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="125" mass="14311" checksum="8E078679978ADACF" modified="2011-01-11" version="1">MAPREKKERKARSALSDVVTREYTIHLHKHVFGHGFKKRAPKAVKAVTEFARKAMGTQDVRIDPGLNAAIWNHGIRNVPRRMRVRLARQRNDDEDSKEKLYTLASFVEVADFKGLDTQVVETDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3L890</accession>
    <name>E3L890_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">OTU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_18649</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178372">
        <property type="protein sequence ID" value="EFP92765.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337184.2">
        <property type="nucleotide sequence ID" value="XM_003337136.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L890"/>
    <dbReference type="STRING" id="418459.E3L890"/>
    <dbReference type="GeneID" id="10539261"/>
    <dbReference type="KEGG" id="pgr:PGTG_18649"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18649"/>
    <dbReference type="HOGENOM" id="CLU_1448379_0_0_1"/>
    <dbReference type="InParanoid" id="E3L890"/>
    <dbReference type="OrthoDB" id="1694816at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22744">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP92765.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="187" mass="21355" checksum="762AF602A8042CCB" modified="2012-04-18" version="2">METDKESEDNPSDGIGGFCENIFTDKEHLEEEPNRSTVKNPLRIRPIKVVLEKPKPTTKADEDDKTPLSKALTWSTHKNVEIDLHKDSIKPAGAIPHILHVHYFKGNGNCGFRAVVVSLGHQSDKWDSISEEMQKEFESNKAYSDQRFLDNVWGAGDNQKDITNSLAWRDKEHQAPLKYWMTFPAHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8C2</accession>
    <name>E3L8C2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Peptidase S8/S53 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_18576</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9 10">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178373">
        <property type="protein sequence ID" value="EFP92797.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337216.2">
        <property type="nucleotide sequence ID" value="XM_003337168.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L8C2"/>
    <dbReference type="GeneID" id="10542510"/>
    <dbReference type="KEGG" id="pgr:PGTG_18576"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18576"/>
    <dbReference type="eggNOG" id="KOG4266">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003559_1_2_1"/>
    <dbReference type="InParanoid" id="E3L8C2"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02124">
        <property type="entry name" value="PA_PoS1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07489">
        <property type="entry name" value="Peptidases_S8_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.10:FF:003882">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000036">
        <property type="entry name" value="Serin endopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000051">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010435">
        <property type="entry name" value="C5a/SBT2-like_Fn3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034187">
        <property type="entry name" value="Peptidases_S8_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF66">
        <property type="entry name" value="SERIN ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06280">
        <property type="entry name" value="fn3_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0134">Cell wall</keyword>
    <keyword evidence="6 9 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 9 10" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="7 9 10" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase S8/S53" evidence="11">
        <location>
            <begin position="152"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="PA" evidence="12">
        <location>
            <begin position="376"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="domain" description="C5a peptidase/Subtilisin-like protease SBT2-like Fn3-like" evidence="13">
        <location>
            <begin position="598"/>
            <end position="714"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8 9">
        <location>
            <position position="161"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8 9">
        <location>
            <position position="211"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="8 9">
        <location>
            <position position="524"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR615500-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF06280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EFP92797.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="893" mass="96901" checksum="0610F788F89D8DE5" modified="2012-04-18" version="2">MLILKARTFPWTVVCTTIALLFLPWIQCGRTGGVTSYPSRFIVTLSPEVVPSLSAFEVHLRNLNISYKILEDYTTLVPDVFYGVSLYLEHSEDVSLIQNSSHVERLNIVKDVFPLQTFKTRILDQPLLATPETYPPHVQTNITDLHRLGILGQGIKVAIIDSGIDCAHPALGGGFGYGKRISFGIDLVGDRFNGTNDPVRGHDPCTKCGLHGTHVAGIVAAQDVGYGFTGAAPKAEIGMYRIFGCGQESQTRDDIILAALLMAYKDGADVINLSLGGSAGWGEGAVLYDVINTLVQKKNAVIIASAGNEGAEGMFMASNPASSKNAISVGSVDATSSVVHYLRMSTGQLIPYRSAIPFPTGSFPVYFTSNSTRIQDDACNPLPESTPDLSKYVVIIRRGTCYFETKVEQAQAKGAKVILFYWNSTIPVGLPNQLSNSSVAVITAEDGKHLLEASHKNNGIRLNFTHSPSFYATSPNGGFMSNFSQFGPSYDFLSPQPALSGVGGDVVSTYPLDKGGYAASSGTSMAAPQIAGVAALILSARGKNLNGLSIRSRLASTARVVRTDFGKGVLESTVHQGGGLVNAFCAVFSNTSVSTSSIALNDSTNMNRSQTFTLVNEGTQPVTYRVEHLPAGSIAAFSPKSQHGRIDSKIPTPSANYASVDIQPDTFTVMPGQKQVIRTKFTPPSGLNPEDLHVYSGYISLDSNVPCETHTVPYYGVLGSMKEQKIIDRGPNVDIDKSRDSRYHFPHFIFSDNNRSSAVEKPLIWDLKKHNHTTFLFRFNFGSPYMRLDLAPHNAVLSNSTNCTNSAWKSKFRGIDLLGLIPTSDSELFSRSGMHEVQELDWNATVLESEYSKPKRIPGGKYKVLLRALKVTGDLENEGDFDRWLSPLIHLTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8H1</accession>
    <name>E3L8H1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">D-xylose 1-dehydrogenase (NADP(+), D-xylono-1,5-lactone-forming)</fullName>
            <ecNumber evidence="3">1.1.1.179</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">D-xylose-NADP dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_18910</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>D-xylose + NADP(+) = D-xylono-1,5-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22000"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15867"/>
            <dbReference type="ChEBI" id="CHEBI:53455"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.179"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfo/Idh/MocA family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.179" evidence="3"/>
    <dbReference type="EMBL" id="DS178374">
        <property type="protein sequence ID" value="EFP92846.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337265.2">
        <property type="nucleotide sequence ID" value="XM_003337217.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L8H1"/>
    <dbReference type="FunCoup" id="E3L8H1">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L8H1"/>
    <dbReference type="GeneID" id="10542652"/>
    <dbReference type="KEGG" id="pgr:PGTG_18910"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18910"/>
    <dbReference type="eggNOG" id="KOG2741">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023194_7_2_1"/>
    <dbReference type="InParanoid" id="E3L8H1"/>
    <dbReference type="OrthoDB" id="2129491at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047837">
        <property type="term" value="F:D-xylose 1-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050984">
        <property type="entry name" value="Gfo/Idh/MocA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055170">
        <property type="entry name" value="GFO_IDH_MocA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22604">
        <property type="entry name" value="OXIDOREDUCTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22604:SF105">
        <property type="entry name" value="TRANS-1,2-DIHYDROBENZENE-1,2-DIOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22725">
        <property type="entry name" value="GFO_IDH_MocA_C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="7">
        <location>
            <begin position="9"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="GFO/IDH/MocA-like oxidoreductase" evidence="8">
        <location>
            <begin position="152"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00042988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP92846.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="408" mass="45233" checksum="C61188DE0F921328" modified="2012-04-18" version="2">MAREQFTCRWGIMGAGAISEKFVRDLLIDPKTRQVSDVHHRIAAIGSRSFVKAEEFISRIEGLESHSTKAYGSYQDLVSDLEVDVIYVGTPHSSHFSDVMLCLGSNRNVLCEKPMTVNAAQARELCRAAKEKQCFLMEALWTRFLPITHSFQKVIASGAIGEIQRVDADLSVDFAPDSLPNDNRLVNPKLAGGALLDLGPYPWTMLALTLLHQNIKPESSSFHDNKHENPAPTDLSIPKISASGTMYTHRNAHPSSEPVDGSVIAVLEFPTPSGRKAQGLLISSMFQNTNEDRAVTIYGTKGRIRIPSLACCPYQFGVTVYPDQAEGDATPTSQGRDNHPQEKIQSFEIPGNAQGYMWEADEVARSIASRKLESKRMPHSESILMMEVFDEIRRQIGLKYPNSIESMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3L9Q5</accession>
    <name>E3L9Q5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">6-phosphogluconolactonase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_19327</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the cycloisomerase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178387">
        <property type="protein sequence ID" value="EFP93280.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337699.2">
        <property type="nucleotide sequence ID" value="XM_003337651.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L9Q5"/>
    <dbReference type="STRING" id="418459.E3L9Q5"/>
    <dbReference type="GeneID" id="10543512"/>
    <dbReference type="KEGG" id="pgr:PGTG_19327"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19327"/>
    <dbReference type="eggNOG" id="ENOG502RIAE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038716_0_1_1"/>
    <dbReference type="InParanoid" id="E3L9Q5"/>
    <dbReference type="OrthoDB" id="9972196at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017057">
        <property type="term" value="F:6-phosphogluconolactonase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001935">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050282">
        <property type="entry name" value="Cycloisomerase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011048">
        <property type="entry name" value="Haem_d1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019405">
        <property type="entry name" value="Lactonase_7-beta_prop"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30344:SF1">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30344">
        <property type="entry name" value="6-PHOSPHOGLUCONOLACTONASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10282">
        <property type="entry name" value="Lactonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51004">
        <property type="entry name" value="C-terminal (heme d1) domain of cytochrome cd1-nitrite reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172531" description="6-phosphogluconolactonase" evidence="2">
        <location>
            <begin position="30"/>
            <end position="430"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005564"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP93280.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="430" mass="46967" checksum="DDF73D5B7F565857" modified="2012-04-18" version="2">MRPSSVLSSSSIIVSLLCSLLGPISLTWARSDISAPMERVHDHSATPLKIVVGGYSGKIVTIEYDQNRKVFQGLKTETRESTSGKSPSFVGFSPDGRCLYATNEVSDFRGLNATGSISSFAITADQSLRHIATALTAADPVALAVSPDGKHLVVAEYTGGSWSKFTLNDQCGFQSENADQRVKYHGSGPNTSRQDHSYIHQVVYNHAGDLLFAVDLGGDSVYVHRVDRRTGDIEEKVVHEIKLPPGSGPRHLSMTGNRAEEYDIYLISELSNQLFTIRLSDHPHHGIKTTIRQETSTLPAGTAEGMRRTYGAGEVMMSGDGRYVYGSNRQTDLSKPLSDNSIVVFKRDPHSGRTSKPAWFPLPGPANTPRHFSFSNDPLQKFFIVGAQQANSLSIFLRNPATGALRFLANTPLQTPAVQLFFPHTQASYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3LA45</accession>
    <name>E3LA45_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19497</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178392">
        <property type="protein sequence ID" value="EFP93420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337839.1">
        <property type="nucleotide sequence ID" value="XM_003337791.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LA45"/>
    <dbReference type="GeneID" id="10537287"/>
    <dbReference type="KEGG" id="pgr:PGTG_19497"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19497"/>
    <dbReference type="HOGENOM" id="CLU_108601_1_0_1"/>
    <dbReference type="InParanoid" id="E3LA45"/>
    <dbReference type="OrthoDB" id="10271139at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172536" evidence="1">
        <location>
            <begin position="23"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="214" mass="23753" checksum="5BFDC38996B87191" modified="2011-01-11" version="1">MQFRSALAFIALLVSLPLTLDAQNLPNDYIVIENVNLAKGGLMRIMSANGMTAFRLIKDLNTPSNGKTTTTLTDGFQKKLFALVSSNDDCKSKTTFTQLEGPVSKDGLPKRAYSYDPRGFRSNVWRFNVYNDAAGARQYYKFIKNRTNKGGIIYRVARGQGDVMVGRLRGQSRQDSWLNVPGGISTFTLSCTEGAPLPELVTMLAYAHIRHDQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3LAB6</accession>
    <name>E3LAB6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19467</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
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                <person name="Szabo L."/>
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                <person name="Schein J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <recommendedName>
            <fullName evidence="4">Cell differentiation protein rcd1</fullName>
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        <lineage>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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            </authorList>
            <dbReference type="PubMed" id="21536894"/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <source>
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    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
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                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178403">
        <property type="protein sequence ID" value="EFP93678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338097.1">
        <property type="nucleotide sequence ID" value="XM_003338049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LAV3"/>
    <dbReference type="GeneID" id="10534967"/>
    <dbReference type="KEGG" id="pgr:PGTG_19339"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19339"/>
    <dbReference type="HOGENOM" id="CLU_128356_0_0_1"/>
    <dbReference type="InParanoid" id="E3LAV3"/>
    <dbReference type="OrthoDB" id="2506572at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP93678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="154" mass="17620" checksum="74201D968F45042B" modified="2011-01-11" version="1">MELFDNIKCSSETEALPGKIIKRIPLPWRSTDFNTLARQLDEIQIQKTHNTQGRQFLNSFSLENKRATSTFSASISLRNVPRGLPRNCYADEYLTMLSEIDVKLLDLKPPIDFPKLLSSTKVSLSIDMNLVCLAKAIKTIHSLIFHGWIPIWFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3LBH0</accession>
    <name>E3LBH0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">VASt domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_19923</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the YSP2 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178416">
        <property type="protein sequence ID" value="EFP93895.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338314.2">
        <property type="nucleotide sequence ID" value="XM_003338266.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LBH0"/>
    <dbReference type="STRING" id="418459.E3LBH0"/>
    <dbReference type="GeneID" id="10535427"/>
    <dbReference type="KEGG" id="pgr:PGTG_19923"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19923"/>
    <dbReference type="eggNOG" id="KOG1032">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_388893_0_0_1"/>
    <dbReference type="InParanoid" id="E3LBH0"/>
    <dbReference type="OrthoDB" id="2162691at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140268">
        <property type="term" value="C:endoplasmic reticulum-plasma membrane contact site"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032934">
        <property type="term" value="F:sterol binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120015">
        <property type="term" value="F:sterol transfer activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032366">
        <property type="term" value="P:intracellular sterol transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13220">
        <property type="entry name" value="PH-GRAM_GRAMDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051482">
        <property type="entry name" value="Cholesterol_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004182">
        <property type="entry name" value="GRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031968">
        <property type="entry name" value="VASt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23319:SF4">
        <property type="entry name" value="GRAM DOMAIN CONTAINING 1B, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02893">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16016">
        <property type="entry name" value="VASt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00568">
        <property type="entry name" value="GRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51778">
        <property type="entry name" value="VAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="513"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="domain" description="VASt" evidence="8">
        <location>
            <begin position="256"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="217"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="426"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="540"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="609"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="687"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="220"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="440"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="488"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="547"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="609"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="628"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="688"/>
            <end position="710"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP93895.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="710" mass="76905" checksum="D1C90700F8F06B9C" modified="2012-04-18" version="2">MQSSSTSSSSTGTAVSSIYGHQPFSSSSINHHPRSSPPDLLEKEDRTSVHTTDSRAYNLSALDDQLGAGYAVASRKRNVDFHAIFKSIPEDDYLIEDYGCALQRDILVQGRLYISEQHLCFNANIFGWVTTLVIPFSDVVTVEKRMTALIIPNAIQVITTQSRHTFASFLSRDATFDLMNSIWNIARPPGSIPTHGLTLDVTMPALDDLSDGTKVAGGYSEDSSASALSHSSATPAPPIKKIDHPPTQCECSPDEHYKELIWDATYPTSPEKLYNILFQSDFLKDFWVNEEHLTEIEVGDWTTSPEAQYPSRSVSYIRPVNAPVGPKTIKCLVSDEHRALDFDKYVSVLSTARTPDAPAGGSFCVRTLTCITWGPNNSSRWLVTAAVEWTKVNRFLKSIIESSAISGQKAYQVGVDQALRKHIQLSQPPEPMSERVESAGGAGNSGGGVGAGLGVGGVKAKGERGRPISMAQAGRSGTVKGTPESRPSDANRTSSKIGSGSGTAVVSLFGLELSWMMLGSIGLVFLLLVWNLLLSFSPPRHHHHHSSSSTTTTTSPSPVHFDDPSQQLLLPPQNQNSKHRHYDQQLSRDVAHTVREALHEYFRDHPILLHSSSPSPSSSPLDNPLSSFEGSTTEPSPLNQDAPGDGDGQSGTENAGVLSPDLEIAQIELLLQSLEIRIRNVRNSLVLNPNPSQPEHSSSSSHTSSPGAFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>E3LBP0</accession>
    <name>E3LBP0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_19737</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178422">
        <property type="protein sequence ID" value="EFP93965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338384.1">
        <property type="nucleotide sequence ID" value="XM_003338336.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LBP0"/>
    <dbReference type="STRING" id="418459.E3LBP0"/>
    <dbReference type="GeneID" id="10535576"/>
    <dbReference type="KEGG" id="pgr:PGTG_19737"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19737"/>
    <dbReference type="eggNOG" id="KOG1607">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1230428_0_0_1"/>
    <dbReference type="InParanoid" id="E3LBP0"/>
    <dbReference type="OMA" id="PWAILEQ"/>
    <dbReference type="OrthoDB" id="2498637at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="96"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="194"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP93965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="225" mass="26309" checksum="BD2EF9EDB20A47E8" modified="2011-01-11" version="1">MDQSQSSANPSTKTLRTRTSSVQLVLNHVTHTPPDHRFAANNFDHSTNLTPPNSSPTQTKSQAKKSKPIPHWEQVSQGFWHDLKTMRWMRHPGTSFRMILIILLPWAFLEQFRFLGWIDSNPLTSFVLISYRLPLQADESYHRYGKGTKDFLFLAFYIIVFSCIRQTVTEYLIRPYARYLALKGTKQDRFVDQGYAVFYWGSATLIGMRVMASQPTWWYNTAEFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3NXF0</accession>
    <name>E3NXF0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_20165</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS989934">
        <property type="protein sequence ID" value="EFP94249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003338620.1"/>
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    <dbReference type="KEGG" id="pgr:PGTG_20165"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20165"/>
    <dbReference type="eggNOG" id="ENOG502QR5Z">
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1321462_0_0_1"/>
    <dbReference type="InParanoid" id="E3NXF0"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47150:SF5">
        <property type="entry name" value="OS07G0546750 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47150">
        <property type="entry name" value="OS12G0169200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP94249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="208" mass="23753" checksum="533D95FD339AEEF1" modified="2011-01-11" version="1">MKDSNGISDFLEECKGLCEGCVLKITLVQQDPKVLAKLTNCILGYKQCPQVDISLNTWVRTAGAGTRLKNPIFWTPTPARSARAGTWVPPGVEDSMGFVEAGGNPPAFETNPQYPLQFPSISPISQTYLRPSPHHSATHIENMMDLRLIQSFEEGYQRLYKDYLAPEPVYVDYLFRRRFRKHCPLFMKIVDQVTAHDRYFQKKPDALW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3NY47</accession>
    <name>E3NY47_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20452</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS990028">
        <property type="protein sequence ID" value="EFP94496.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003338867.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NY47"/>
    <dbReference type="GeneID" id="10527790"/>
    <dbReference type="KEGG" id="pgr:PGTG_20452"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20452"/>
    <dbReference type="HOGENOM" id="CLU_2177120_0_0_1"/>
    <dbReference type="InParanoid" id="E3NY47"/>
    <dbReference type="OrthoDB" id="10462684at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP94496.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="110" mass="11775" checksum="D7893C77F0105E74" modified="2012-04-18" version="2" fragment="single">TNDVGLEVSGLKSCDIRGVTASDRAVRVRPICAHHELSSQRPICAHPHLSSTSDLKRAGIQTLPPFGFHQLAQESVQRQSDAPNRISSGPDGFYTDPTTTAGLSSRVGPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3NY56</accession>
    <name>E3NY56_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20461</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS990035">
        <property type="protein sequence ID" value="EFP94505.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338924.2">
        <property type="nucleotide sequence ID" value="XM_003338876.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NY56"/>
    <dbReference type="FunCoup" id="E3NY56">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3NY56"/>
    <dbReference type="GeneID" id="10527881"/>
    <dbReference type="KEGG" id="pgr:PGTG_20461"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20461"/>
    <dbReference type="HOGENOM" id="CLU_2016337_0_0_1"/>
    <dbReference type="InParanoid" id="E3NY56"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP94505.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="123" mass="13106" checksum="1181124856605279" modified="2012-04-18" version="2">MVLENEKVHPADWENPGRVKSTLCYVKIGLLLRPLETVGLKLPPPTAKDQNSTPLPNINHLPYNSPAVSHGFLAMAEAEAQKPTESSGTAIEDAPGSKNKGTKGPGDTTKNSKAAPKKKKGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPM0</accession>
    <name>H6QPM0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Concentrative nucleoside transporter C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_20793</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EHS64090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890499.1">
        <property type="nucleotide sequence ID" value="XM_003890450.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPM0"/>
    <dbReference type="GeneID" id="13542935"/>
    <dbReference type="KEGG" id="pgr:PGTG_20793"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20793"/>
    <dbReference type="eggNOG" id="KOG3747">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016813_1_1_1"/>
    <dbReference type="InParanoid" id="H6QPM0"/>
    <dbReference type="OrthoDB" id="6075923at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005337">
        <property type="term" value="F:nucleoside transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015293">
        <property type="term" value="F:symporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901642">
        <property type="term" value="P:nucleoside transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008276">
        <property type="entry name" value="C_nuclsd_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011657">
        <property type="entry name" value="CNT_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002668">
        <property type="entry name" value="CNT_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011642">
        <property type="entry name" value="Gate_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10590">
        <property type="entry name" value="SODIUM/NUCLEOSIDE COTRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10590:SF4">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 28 MEMBER 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07670">
        <property type="entry name" value="Gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07662">
        <property type="entry name" value="Nucleos_tra2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01773">
        <property type="entry name" value="Nucleos_tra2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="128"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="179"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="209"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="232"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="290"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="326"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="377"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="454"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="557"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="594"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="domain" description="Concentrative nucleoside transporter N-terminal" evidence="9">
        <location>
            <begin position="213"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoside transporter/FeoB GTPase Gate" evidence="11">
        <location>
            <begin position="296"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Concentrative nucleoside transporter C-terminal" evidence="10">
        <location>
            <begin position="397"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="29"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="50"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS64090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="615" mass="68421" checksum="9F0011EB12631748" modified="2012-04-18" version="1">MESSEQQDKSDLNALENQGQVNRTHHTGRSSAESITHSFPKSQEQIIIGETEKPISDSRDVENDNTSEKSQNDARTGKQPPQWWPSLIKWNMRESTGRYKRMAIHLFFVSILLGWWISGLVLKETRHKWVITTFWTWLILLIIFFRWVPTKYVTRPIGKTYMTLIGNPIMKSTNHRVRLSIGWLILLGLWLGSTFGIKLAQGSNYTGRLRSLVGVIVFQLGFWASSRTKSLIRWRTIIVGLILQQLLALVVLKSKAGYDFFNWIAKAATDLLDQGTKAGGFFFSSQVLEYHWYFVNTLAAIIFFIALVQLLYYFGVMQAIMIKFAWVFYTLMGVSGVEALTAAASPFIGQGGSFVLIRPFAAQMTAAEIHQVMTSGFSAISGAVLTAYVAMGIPAVYLINASTMSIPASLAISKLRFPEDSQPLTAGKVIIPEDEHTNRKDSNAIMVFSDGAWLGLRVAGTILANLLTILALSYTVDGLLTWIGQFWGMDPNGAHPLTLELIFQYLLYPAAWLMGTPNQDVLKVSRLLAIKLITNEYVAYRELSKLQPEMTEKGTLIAIYSLCGFANLGSLGIQIGVLNSLVPSKKHVISRVAISALLCGFFSTIQTAAIAGMIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPS6</accession>
    <name>H6QPS6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_20925</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EHS64313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890469.1">
        <property type="nucleotide sequence ID" value="XM_003890420.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPS6"/>
    <dbReference type="GeneID" id="13543012"/>
    <dbReference type="KEGG" id="pgr:PGTG_20925"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20925"/>
    <dbReference type="HOGENOM" id="CLU_3088332_0_0_1"/>
    <dbReference type="InParanoid" id="H6QPS6"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="52" mass="5965" checksum="4EF57001714CB53D" modified="2012-04-18" version="1">MYARFQDSFTCSANKNDSKPWKCCEATNFRPGTVRYDITESLKNCRTPTPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQB7</accession>
    <name>H6QQB7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21077</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EHS64819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890340.1">
        <property type="nucleotide sequence ID" value="XM_003890291.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQB7"/>
    <dbReference type="GeneID" id="13542137"/>
    <dbReference type="KEGG" id="pgr:PGTG_21077"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21077"/>
    <dbReference type="HOGENOM" id="CLU_2334671_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQB7"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="98" mass="10903" checksum="075A2BE743790FCB" modified="2012-04-18" version="1">MSLPTDFNLIINSNPSIHGWVVIDKTNPELIFCCDNRSANFSAVLIFPEGTPNTPPTNIEVGQKIWITGHIFKHEKDLVYVAATTVFFNSADTPPIFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQC3</accession>
    <name>H6QQC3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_21083</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EHS62534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890247.1">
        <property type="nucleotide sequence ID" value="XM_003890198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQC3"/>
    <dbReference type="GeneID" id="13542241"/>
    <dbReference type="KEGG" id="pgr:PGTG_21083"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21083"/>
    <dbReference type="HOGENOM" id="CLU_044887_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQC3"/>
    <dbReference type="OrthoDB" id="2498926at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="42"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="188"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="364"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="391"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="245"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS62534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="463" mass="51607" checksum="9BEFCE930E154A13" modified="2012-04-18" version="1">MASKESALSYERLLGIVPVGVDPYAFIKDFITTWSSVPLSRITLAFLMIFFGLHVLIAGICLIILILPHTRGANRSQWVLRKLYIQEQSGREAHQAPLYFANARVLLTISQLLSSVSTPAFIWFDTGITTPNEHSLRPQVMPAFGLMNLAEILAYWSLSHCFLVTVCYGHKSQGDNSTGLIRYISPRLINFLFICFPIAATAMTIVVVTRVIVVTNGVQSGMVQLFAGLDRGSSLWKQIAVKSQSSVAQAQLNQLNLELKGLANETALLLETSFERFRFGECTFLFFSSLSCPVFIVLFFVLLRNYQHQRRNLSTKLFRPGNASKHENTHSDSDTQCHSSAANMGYFEAIRTDRQFLHLNMRALGTFIAMLVNIIVHLIAIFRTVDTLAVPYWRGVVSCLATGGSTFSGIPIAWQCWRLYADQVHSSAQASKNDVSTLEELPKTTVFSGACDESASEIHVFQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQF3</accession>
    <name>H6QQF3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_21111</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EHS62564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890277.1">
        <property type="nucleotide sequence ID" value="XM_003890228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQF3"/>
    <dbReference type="GeneID" id="13542196"/>
    <dbReference type="KEGG" id="pgr:PGTG_21111"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21111"/>
    <dbReference type="HOGENOM" id="CLU_038852_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQF3"/>
    <dbReference type="OrthoDB" id="2507145at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003605726" evidence="2">
        <location>
            <begin position="20"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="443"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="453"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="468"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="505"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="531"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="572"/>
            <end position="592"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS62564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="592" mass="66283" checksum="B3C4229CF5FDD40C" modified="2012-04-18" version="1">MSCSLRLLKSLSHLYLVHSYYFFGGVSSVQADVAEVQEHEHQQEDGDMVKDMVSKATRRMKKIQPEPPNNNTSQPPNKNTSQGGSTGGADSASANNGQSALEELSVTQTEHLSIAEGSKDKGPTSASSSTEDAINDAASKDNEVVEVQLTKDKQSDTVTAYRNLLSRRESDGPLLVVNKAPDLHTPRSVLQSLTDREKLWEKIVESKLANDEANADFLLRIYLSLPKEEITKTLRVPVELSELRSTSADVAIPTQRANSVDKTVQFIKGSVPNHCDIGFTPFFDKNIREFKGPLPLTIFDREWQEDAVNFHSGKKSKSDEKDGVYTGYEYPNEWSQSFSAWSNNFRSFLMTYRDIYKIPEFAEWIVAHKANIDEIIANEGFLTGFRYDMIIQANTFAYRVETSNGASVVDISVMRKDVQEKAWAVTRKLDELDFKDNPYAQGGIKAGFDPKTGRPKIGKNHKSFTDASPYSSGPNSSGYGNSSRGRGGFRKKSFGRGGYGYENGPDDRRDEDFRGWGDRRFNNQHRFGGQDNYTQNRFSHNQGYSGNQAYNKDEGYKSHPAPAPNTFRNNHQNKDLAMKGSGKREQKDGKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQS2</accession>
    <name>H6QQS2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EHS62820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890139.1">
        <property type="nucleotide sequence ID" value="XM_003890090.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQS2"/>
    <dbReference type="GeneID" id="13541438"/>
    <dbReference type="KEGG" id="pgr:PGTG_21141"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21141"/>
    <dbReference type="HOGENOM" id="CLU_1540838_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQS2"/>
    <dbReference type="OrthoDB" id="4021778at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026749">
        <property type="entry name" value="Tmem135"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12459:SF15">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 135"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12459">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 135-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="174" mass="19013" checksum="E51626DD040AE28A" modified="2012-04-18" version="1">MILRGTGLGDPITGTDLDGRVAPQLGSLLAGFSILIENKKRRGEMALYVATRALCATVDEILPRWLRRRIIVNRWVSIWVERISFSVSIGIITCAIVHHPDYVRGIVQGILKYAIGPDWPKEPFHPSSPPPPPPPATAVASAIQNDPTLPVSSNQTHLDDSTEEVVSYDASLQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQX2</accession>
    <name>H6QQX2_PUCGT</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21284</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EHS62935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890095.1">
        <property type="nucleotide sequence ID" value="XM_003890046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQX2"/>
    <dbReference type="GeneID" id="13540865"/>
    <dbReference type="KEGG" id="pgr:PGTG_21284"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21284"/>
    <dbReference type="HOGENOM" id="CLU_3125682_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQX2"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS62935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="50" mass="5743" checksum="30708BA19CC9CA1C" modified="2012-04-18" version="1">MANARFVLFRVEWTRARRVSAVSDLTFLSHYNTDGVARGTTRSTIRPEET</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRS1</accession>
    <name>H6QRS1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21543</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EHS63368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889806.1">
        <property type="nucleotide sequence ID" value="XM_003889757.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRS1"/>
    <dbReference type="GeneID" id="13543054"/>
    <dbReference type="KEGG" id="pgr:PGTG_21543"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21543"/>
    <dbReference type="HOGENOM" id="CLU_1886771_0_0_1"/>
    <dbReference type="InParanoid" id="H6QRS1"/>
    <dbReference type="OrthoDB" id="2508359at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="135" mass="15227" checksum="9F704BEFF8ACA7FE" modified="2012-04-18" version="1">MIGCSEVYHGDELVDEWIEKPEGFALEGVPWVLYPFSAKKGPVNYLNMAVKPRWAMLRISGTGTEEHMRLFNIGLEPLVYAVKETDSNRWIWAKQLGPTQGHLIRTSAALVYLLPIPVVKIDDKSSELGQKQSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRX8</accession>
    <name>H6QRX8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21593</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EHS63462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889747.1">
        <property type="nucleotide sequence ID" value="XM_003889698.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRX8"/>
    <dbReference type="STRING" id="418459.H6QRX8"/>
    <dbReference type="GeneID" id="13543110"/>
    <dbReference type="KEGG" id="pgr:PGTG_21593"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21593"/>
    <dbReference type="eggNOG" id="ENOG502SBYH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004591_1_2_1"/>
    <dbReference type="InParanoid" id="H6QRX8"/>
    <dbReference type="OrthoDB" id="2506480at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="856" mass="98387" checksum="B506F4BEEE43C78E" modified="2012-04-18" version="1">MDLDPVSDQPMQAGLPEHLPQEQPMAYTIADGEDVGFGSDPSEVEEAPSDYSEEFHSWEVFGDEDVPEATQEDAQSSRSTRHDWEPFRSQEDFMACLMTGYLRNMLSRVTYLLLRLILAMKNLILPHWDSVRRTKQRIRTMLNLTTKESLSVWNNKLFTLSLKGILANELLNPLVTSNLEFYPHDPAGSQIDSLYQCFKWREDLSRDLRVQMIYIGNKHYYIFEPVRLTSGEVIVPIYFYKSNGGIYAKCLTPHYEPTKGGKGLKLVIPSDIKFCDQALQTIDIKEFDEMYSELYLEDNVSLADACGGRMYEDLNGVESEIVLPNPWRVKANGKIIRHMPITLYSDDTSGNTSKQWNKHISFYFTLAGLHPRMTNQEYHCHFLGTSNVAGVLELAEPIVEEMNELATNGHFAYDSLLKQDVLIMSVIMAFLADSPMHAEITSTPLPANANSPCRVCQLSVARKVDKSLKSYVMDLMGLSDGIRQAPPPRNWADTKSRLHSAWKDSKTNAKTSYDDKIREHGLKDNINVEFVEKYHQRNKPGQKESIDRIDKLTFDRLFSPFLNLKGFDGTKDTPVEILHVFQLGIIRYLLRDFMKGLTAGDKKLLEARWRAFNSESLNVPPIQSLFMVNHYKSFIGKHMKIVIQAAPFVFFPFMTTQQRELWRSLCLLCTYVYQTKIDDMDLYIADLQHHIDMFMYHVLSMTAQWVNKPKFHMLTHLADAVRRFGPPCLFASEKFESFNGILRNASIHSNRQSPGKDIAITFSNYQGMRLILSGAFLFDHQRMYHFQPSLNVTEVFRTNKGVQNMMGIDLSYEPKYPILIKGETSQAKLDQTPTPHYLMNAYPAARWKLIRKIQTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRY2</accession>
    <name>H6QRY2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21597</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EHS63466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889751.1">
        <property type="nucleotide sequence ID" value="XM_003889702.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRY2"/>
    <dbReference type="GeneID" id="13543128"/>
    <dbReference type="KEGG" id="pgr:PGTG_21597"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21597"/>
    <dbReference type="HOGENOM" id="CLU_069873_0_0_1"/>
    <dbReference type="InParanoid" id="H6QRY2"/>
    <dbReference type="OrthoDB" id="2506388at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="342"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="342" mass="39318" checksum="6CCF6F1CAD624891" modified="2012-04-18" version="1">MKYNASRTLRLAASDTGNPSSTWEEDSASLYNSAHQIAALTTFTELATTSAYSYLKIDRELAQDMTLLIPAYNHFVHYLQYNRYKREQKEKGKASQEAAKKKFNKNRERLRNERRDFAIINKFPKQYRDILEPITAHSNDEKVEGKGFYKIKTLPYRSNNTNRFFRRLDIMMKNAAEQDPIAKSSRRRVRRLPKNPEMSSYKIAPKGLPIDFYHPKWYHNLVPAQQQSIPNQKALAFLPDANESLLPKDDDEAAEEEQGGNDDDEGEGIDLTQPSPDNSDDEFHVEGDAGDLYEEEDDGFVVNDGEEQDGSDDDDDDYDKAEDGGVKEDCQMDDIPECEEDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSW4</accession>
    <name>H6QSW4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21937</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178301">
        <property type="protein sequence ID" value="EHS63918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889386.1">
        <property type="nucleotide sequence ID" value="XM_003889337.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSW4"/>
    <dbReference type="GeneID" id="13540689"/>
    <dbReference type="KEGG" id="pgr:PGTG_21937"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21937"/>
    <dbReference type="HOGENOM" id="CLU_2050812_0_0_1"/>
    <dbReference type="InParanoid" id="H6QSW4"/>
    <dbReference type="OrthoDB" id="10489800at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="120" mass="13412" checksum="156BECB8B9DDA479" modified="2012-04-18" version="1">MALWETYSSSAKITCLIFLSKEASSKAQSTPVLSAESSVFENKPFKENNRPVAICNQYLCYQQLRCTRRSFEIEVGGFQKSLDQLIPCNLKPKFVVEEVQEATGVGDEKFGVVGPDRDPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTH2</accession>
    <name>H6QTH2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22096</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178313">
        <property type="protein sequence ID" value="EHS64187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889203.1">
        <property type="nucleotide sequence ID" value="XM_003889154.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTH2"/>
    <dbReference type="STRING" id="418459.H6QTH2"/>
    <dbReference type="GeneID" id="13541185"/>
    <dbReference type="KEGG" id="pgr:PGTG_22096"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22096"/>
    <dbReference type="HOGENOM" id="CLU_2764887_0_0_1"/>
    <dbReference type="InParanoid" id="H6QTH2"/>
    <dbReference type="OrthoDB" id="1938138at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="70" mass="8231" checksum="9861A63A46AB1C48" modified="2012-04-18" version="1" fragment="single">MATPVFQRTALDPEEAYFIMTKIEELGFIPNKYVQGFRTLDTDKKFQDLEPNISTTLPIWMVIELHKKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTQ3</accession>
    <name>H6QTQ3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">REM-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_22148</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RICTOR family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178319">
        <property type="protein sequence ID" value="EHS64268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889122.1">
        <property type="nucleotide sequence ID" value="XM_003889073.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="H6QTQ3">
        <property type="interactions" value="260"/>
    </dbReference>
    <dbReference type="STRING" id="418459.H6QTQ3"/>
    <dbReference type="GeneID" id="13541744"/>
    <dbReference type="KEGG" id="pgr:PGTG_22148"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22148"/>
    <dbReference type="eggNOG" id="KOG3694">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001013_1_1_1"/>
    <dbReference type="InParanoid" id="H6QTQ3"/>
    <dbReference type="OrthoDB" id="271111at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028268">
        <property type="entry name" value="Pianissimo_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028267">
        <property type="entry name" value="Pianissimo_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029453">
        <property type="entry name" value="Rictor_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029451">
        <property type="entry name" value="RICTOR_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029452">
        <property type="entry name" value="RICTOR_V"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13298">
        <property type="entry name" value="CYTOSOLIC REGULATOR PIANISSIMO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13298:SF11">
        <property type="entry name" value="RAPAMYCIN-INSENSITIVE COMPANION OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14663">
        <property type="entry name" value="RasGEF_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RICTOR_V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="197"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="domain" description="Rapamycin-insensitive companion of mTOR middle" evidence="4">
        <location>
            <begin position="773"/>
            <end position="998"/>
        </location>
    </feature>
    <feature type="domain" description="Rapamycin-insensitive companion of mTOR" evidence="6">
        <location>
            <begin position="1166"/>
            <end position="1238"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="143"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="724"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1386"/>
            <end position="1427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1446"/>
            <end position="1503"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="49"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="114"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="9"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="156"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="736"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1391"/>
            <end position="1406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1407"/>
            <end position="1421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="1448"/>
            <end position="1463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1464"/>
            <end position="1487"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01307"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM01308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS64268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1517" mass="168843" checksum="AB0E71D9E997879C" modified="2012-04-18" version="1">MDRHQQQQPSTHSNSFSSLNHLNNSNNNNPSSASQLALDSPEPPITPSKDHLKEQLAALRIKLERAEAIQRGAENLLHQVGAGQGLKRKDNDLSSQLNNSIGGCSTTTTASTLHASVEAELDLANRNVKALVKEIEALEALSATSPRKGRRRLKTNDSAQSNPPSQFYADDNLRIQSEIEELIKQFVDEDSFSEPQLRKLSDLVKSQRGYSKLNRRKLIDIGLLGLSDSNPTEVRAESYRLLRHVVHTLVDMNVLRLSRIEFIIVKTLTRDHKYDYEKLQALILVRSVACYPRFLTLSVARAIVSIAETAEEKLRQLAIETLGELMVRDFALLSQADGIKVIMQSLTDTSGGLKELAPYMASLVMAMVDRPVCRQYLKPGVDFESALAAITEVNEPSSSQAQDERVRTSVKLIVLLLKSWTGLMFLCLNKKRSLRMYVQSMKVPSDSIRLILIDALFEIFQIKTPQWFSTFMSGKRLTYYQKPGQQPVQSSQSPINKDLPEKTSERATLADHYLAILLLVFFDVKLLETLIEITESRGTRDEPVPGSTTTKQANLNIASASKSSVVRKKASLLIAEILDLSNRILPLHYGTRIQSLPRLFELAASFQQGDHRSTGTAALREVDSLHRTKNRLASPFLAKQSNPGTSSSHWDDVSLFRSFGIGSMGLNTVGGVSGSATATSVNGLLSGVSNSEGASKVGGSGVMGSSASVNGLASGLSLITGGSSTTTGSGGGTSGLGSVSSVTGSANAGSDPPQNAKLLPHSDPRFRLHFMNIDDVSFRNLVIETQVLVTKDHTRWNIVKLLELIEGTLYMNSKRLEELTRSTKVMKRLLGFLHPFEGRYPYIKRTKHTSRYTKLACSTLQTLLSDPVGVKFLSEDKLVEQIGLGLRQLDQHAGAQSHEVLFSQSRVEDTLTHGYFEMLGVLSKYPEGIRLMEKFKIFTSFYALCEMRTRDDLVKQVIENVDYSINGHCRLVLSKALTSSYKHVRLFATHHLEKLISRAEFSVSASPYQHVNIAPANSPTAIKDAKSCFVREYSRAVLLDRSPRTVSNETFVEILMMFLSTSVGARYLKEIEWTEREMDDWFRERNRDYVIQLEVYLSKLLNADGTRDEEDDELIASVDGSPPPHFYGELVKTAEGREMLTSKGHFDEFVKTIRKHGREENDLEVINEVKTALWAIGNIGASVGGLTFLETECVVEDMVSIAESSNVFSVRGTCYFVLGLISSTTEGSELLEDAGWATVLTPLGMITGICVPTRLNEFIVNPTWKPKKKKLPDLSTRYAPFKSPIHRKILTSIANLSNHLLANNSSKTLTRLKTKYREAFQDLGLFHRALKILGNYHYRLVTRRYIMELFGDIKLDSTSARKIHEAGESMKISRADISRTITQDISSLQINKEEPTKPHEKHHDLEDRDEEEEDEDEEIETYESVRMEDLPVRSGSCLLEAYNKTDYCSDDDEDGDGEDEEGEANTTANGTSLVHPNQIQNSSTSRPATLVPPRASVGNLKPNDRLPIVTIRGFRVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>H6QU25</accession>
    <name>H6QU25_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22301</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178327">
        <property type="protein sequence ID" value="EHS64430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003888944.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QU25"/>
    <dbReference type="GeneID" id="13541019"/>
    <dbReference type="KEGG" id="pgr:PGTG_22301"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22301"/>
    <dbReference type="HOGENOM" id="CLU_2942863_0_0_1"/>
    <dbReference type="InParanoid" id="H6QU25"/>
    <dbReference type="OrthoDB" id="10274876at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="60" mass="6588" checksum="30323E89DE1BF531" modified="2012-04-18" version="1">MLLMLGEHNQLGDLGQVWDTPAATGSPVLLDMSAPEDLSLIDRIARCLETFYQSRVKAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQA6</accession>
    <name>E3JQA6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00240</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307290.1">
        <property type="nucleotide sequence ID" value="XM_003307242.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQA6"/>
    <dbReference type="GeneID" id="10528017"/>
    <dbReference type="KEGG" id="pgr:PGTG_00240"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00240"/>
    <dbReference type="HOGENOM" id="CLU_2265012_0_0_1"/>
    <dbReference type="InParanoid" id="E3JQA6"/>
    <dbReference type="OrthoDB" id="10268457at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172872" evidence="1">
        <location>
            <begin position="25"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP74284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="103" mass="11346" checksum="C38F111C4DB6B7CC" modified="2011-01-11" version="1">MSRFLRLIASVLVMASLGVTGGNSQTSYFGCKRDVDGVCSKILPSGLETRLVWAIRLHRKKRDYACYDGFYPQCCMQGKYQDIDKGPMTIPSGPVPYCDAGGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQN7</accession>
    <name>E3JQN7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_00642</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74686.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003307644.2"/>
    </dbReference>
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    <dbReference type="FunCoup" id="E3JQN7">
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    <dbReference type="STRING" id="418459.E3JQN7"/>
    <dbReference type="GeneID" id="10546488"/>
    <dbReference type="KEGG" id="pgr:PGTG_00642"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00642"/>
    <dbReference type="eggNOG" id="KOG1338">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_017135_0_0_1"/>
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    <dbReference type="OrthoDB" id="341421at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016279">
        <property type="term" value="F:protein-lysine N-methyltransferase activity"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
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        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="entry name" value="Ribosomal lysine N-methyltransferase 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1420.10">
        <property type="entry name" value="Rubisco LSMT, substrate-binding domain"/>
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    </dbReference>
    <dbReference type="Gene3D" id="3.90.1410.10">
        <property type="entry name" value="set domain protein methyltransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rubisco_LSMT_subst-bd"/>
    </dbReference>
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        <property type="entry name" value="Rubisco_LSMT_subst-bd_sf"/>
    </dbReference>
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        <property type="entry name" value="SET_dom"/>
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        <property type="entry name" value="SET_dom_sf"/>
    </dbReference>
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        <property type="entry name" value="SETD3_SETD6_MTase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271:SF34">
        <property type="entry name" value="N-LYSINE METHYLTRANSFERASE SETD6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13271">
        <property type="entry name" value="UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Rubis-subs-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81822">
        <property type="entry name" value="RuBisCo LSMT C-terminal, substrate-binding domain"/>
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    </dbReference>
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        <property type="entry name" value="SET domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50280">
        <property type="entry name" value="SET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SET" evidence="4">
        <location>
            <begin position="93"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP74686.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="622" mass="70585" checksum="2A8AF4D563877EC1" modified="2012-04-18" version="2">MRKTVLSLVWTDTFKTAPVSAWVGSENLLPRASRCRSGISREGQKADMFWPKRGPEFLKKFSVRMDISMDSNNSTGPADRRYEKFLEWLRKARGVELEETISAHEFRETGEGWGLIAETEIRKGTTLFSIPRPGPSANSPLLSVQTSRLMAVLSAQERARISRNWLPLLLVLLWERVQAILNPLDPLRWAPYFDTLPEEFDTLMFWNQDELAELTGSTILDKIGKEEVEKDYETVIKPMIESRADLFPVPEGTSWEENYGIAMYHRMGSLVLSRSFHVEASPSNGEDPDEGHDVSMESAHSLVPESVDNGHSTEPIQETLDHHSELGIEGEDEDEDEEREAVEDIAMVPLADLLNAKTGSENARLFYETDCLKMKATRNIKKGEQIYNTYGDPPNSDLLRRYGHVDDPNRFDVVEISIKTCVDVAQSHLSSQSAPMVSSEGLQHRLDWALEMGIDDVFEVATEVERKKNGSPLLPEELTCLLKILLLSGEEFEDHKSKEKLPKPKLNNPDVITLAQKVIDRRMKDYPTSIDEDERLLEGLRSTSRHSRAGDSLNRRIKAIIVRKSEKQILAQLRDSLVQKLGSYQQGQDQNGVHHLQKRKINCDTDLDLNQCQAAIKSRKTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQP9</accession>
    <name>E3JQP9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00654</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307704.1">
        <property type="nucleotide sequence ID" value="XM_003307656.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQP9"/>
    <dbReference type="GeneID" id="10527490"/>
    <dbReference type="KEGG" id="pgr:PGTG_00654"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00654"/>
    <dbReference type="HOGENOM" id="CLU_1411981_0_0_1"/>
    <dbReference type="InParanoid" id="E3JQP9"/>
    <dbReference type="OMA" id="HAPECRT"/>
    <dbReference type="OrthoDB" id="2502260at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP74698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="205" mass="22745" checksum="DA6D35D4282AF074" modified="2011-01-11" version="1">MSHVRRFSESSVSDIKSESSPANYYSLWVTKHPSGPRTVRVSRSSFDFGLRAGKGGLRSLGIGSFLCPTLEEEEMSGSGDETGLVESEKTDDTAHSDECLKSIDGDDQLRKADVTAPSPRLPIPHPRSTTGLKVLIKRLSLSLLKTRSFTRTRPEPELPIIHSLDHPGPSRLDPHALVFGDENPERIEQRSVEFYQAFFPSLILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3JSB8</accession>
    <name>E3JSB8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">ZP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01436</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP74843.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319262.2">
        <property type="nucleotide sequence ID" value="XM_003319214.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JSB8"/>
    <dbReference type="GeneID" id="10546959"/>
    <dbReference type="KEGG" id="pgr:PGTG_01436"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01436"/>
    <dbReference type="HOGENOM" id="CLU_095050_0_0_1"/>
    <dbReference type="InParanoid" id="E3JSB8"/>
    <dbReference type="OrthoDB" id="2495475at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173158" description="ZP domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP74843.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="251" mass="27817" checksum="2DD08097B5BA33C7" modified="2012-04-18" version="2">MVSHRFIFIAVICLLNAPSSLQLTKPVPLTDIKCSKKRQLRPGDCARAYQKIIYDTDLTLDQSEITLEKVSGSCVTRIDNPKFLNVPKEIIEDAFNQVVTKCDGVPGHANLTSFDGVRVLVRHHTRPSVYGYEDDTELNQSICFRDSDTQDIVEEDCMEAYRLLPTDTAGHFLSVEHHVQGNSIGLTFKTCLVSVWTSDGSKIIVLKGDMERLFGKLMQQCKVNGKGGTLITEGAQGKNGKVNLQVSTPKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3JSW3</accession>
    <name>E3JSW3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_01731</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319557.1">
        <property type="nucleotide sequence ID" value="XM_003319509.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JSW3"/>
    <dbReference type="GeneID" id="10547580"/>
    <dbReference type="KEGG" id="pgr:PGTG_01731"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01731"/>
    <dbReference type="HOGENOM" id="CLU_018552_2_0_1"/>
    <dbReference type="InParanoid" id="E3JSW3"/>
    <dbReference type="OMA" id="FSHAREY"/>
    <dbReference type="OrthoDB" id="3246760at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF85">
        <property type="entry name" value="GH03217P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="9">
        <location>
            <begin position="162"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="332"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP75138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="374" mass="42705" checksum="2CCDC77334B05D45" modified="2011-01-11" version="1">MLFEDEAMVSDLIHLILSKRYLNNRLPARTHNEYNLALLFDMRDEDFKQAVRTTKTGFTWLLEQIYFNAIFYSNSPRPQLPVPHQLALTLERLGSNGNGASVGRFSRNLSVGRGTVIKASRRVIRAINDLSPRYLLWPDKERRDEISKVMKAEGFEGCIGFVDGTTIPLYQRPSIDGEVFFDRKKRYSINCQVICDCDRFITAYMTGWPGSCGDSMVFKRMMVHKEPALFFDPGQYLIADSAYELGLHCIPAYKAPAAYIQENTEFNYCLARSRVRNEHTIGILKGRWASLQHLRLAIQKPSDMMEIIRWVNCCVTLHNMLAHLGDAWDSLEQSTEDGPATAARGSPEDTAKEHRELVQAHCLEKNYLLGVLPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3JTJ6</accession>
    <name>E3JTJ6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_01914</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319740.1">
        <property type="nucleotide sequence ID" value="XM_003319692.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JTJ6"/>
    <dbReference type="GeneID" id="10542787"/>
    <dbReference type="KEGG" id="pgr:PGTG_01914"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01914"/>
    <dbReference type="HOGENOM" id="CLU_1960629_0_0_1"/>
    <dbReference type="InParanoid" id="E3JTJ6"/>
    <dbReference type="OMA" id="APREIHA"/>
    <dbReference type="OrthoDB" id="2497011at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171518" evidence="2">
        <location>
            <begin position="17"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP75321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="120" mass="13559" checksum="CAB14B0F58B5A754" modified="2011-01-11" version="1">MLHLIRTLFSLTLVLAFTVVAYPSQSALLQNAPKGLGKRFQSIRPEASAPRPWVKTARDFLQEAPQDTTAQRLEKRSIQPRSFSKIRRRVEAGIATSRQTTGLSNPRPWVQKDEVISSMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUG4</accession>
    <name>E3JUG4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL3m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_01020</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
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</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
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        <lineage>
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            <taxon>Pucciniomycetes</taxon>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
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    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="PANTHER" id="PTHR48125:SF12">
        <property type="entry name" value="AT HOOK TRANSCRIPTION FACTOR FAMILY-RELATED"/>
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        <property type="match status" value="1"/>
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        </source>
    </evidence>
    <sequence length="1463" mass="157628" checksum="E5D3581922C9A7C3" modified="2012-04-18" version="2">MNQQEPQQPQQQHKIEYFDLNHHHFINNNNQQQQQQQQNSTTTISFPSTASTATTQTTATSAATSASSSASSTDQTHLILIELDQRFHAFRTALLQEPRQRVQLLKDELISTIDQATIKTNPPTTTTQLNHHHHQQQQPITYIEHKTNFPLKLERKRSRQRLDSDRTTHNPKAARSDGPINSSAGLLSRRTSQIGITSTPNGLTEFVGIPDLLSPPINTNTTTTIYSDQSSTTNSNQSSQFSTHPPPPQFHSLPLSQATRQLPPPKTPADSPNPTHHHQPLHLHHHHPPSSCYSASATPLISPHDLSLPPQNSENSLQAILSNPKPQTHQPPSDLSLPLTEHHGNLTGSHEAQDGGKSDPLHLQLSGIIPDLSYQQQLTDQKINNLSANSTDLSALNSPELVESGDRELDRLHMLPSDRPGKFTFTLPSQLSPPIITENTRFGRSRPLVRRHSKSFTSPRILGSDLHSCSRIVTEPNNSKLPQTPYTFSSPSLLPVGARIDLSSLPSPISGLPTTSTISLEPTQDEIMQDSMATDQMSIQPETLPPSEQIPNPLTDRPQTARSRAATMESQRPSSGADFTIGDPDTSLTIEDMAIDSVDPPTHPVLDHPLHSPPSPSAMPPPGSDPSLLLPSSSIPGPTHSEDRAQVLIPPTRVPSPPVNRQMGAASAGVSSHLAAQFEPIFTQWLGRLCSDLDMKDSKGEPIHQTLMARKMQRLEESADFRPFKFRIQAFTNSFFEELVRNGFQEKDLPMKKVRQYLWSQSCISRFNEEGKKAKSKGNHVWHIEAKKLMNGQWSFLHFQRKIIGTPSPEAYAGIKWTWAAKVWDPQCSAQNIKASFSSPELPNWLSWRGNLLTGVPTVDLIGRSYHIIVRAITNHNSKPSSLDTSFNLSIKSPDEMVTSRKNRKPTEVLASQSDSTLPQSSSEQISLNTTPSHSPPDLSALNSSALEPAPPTGMIISEGNRSTSYSQVPALSRTDSSESVPISSPSLEDPPRLGQAAGFDGILTENSLMHDFRAGALKEDESLEFDSAHAADVLNGMMGGTPAATTTTTTTTTTTASTSEAVVEAFGQLSENNSLQAGSLVVPKSQSIDLSSSLSSAHPLALHLDQQIGLRAAPTDQQARNLMGAGEGQLTSGMMMIASNGGMVVTSELEADGSSGGGPTTRGDTLESSGLTEAVGLLDRMMSPAQLMAPTMGGTNEMETTTGGSELVVGLSSGAEMCVPPASSELSTRMILDQPVQILDAVVHHHHQQQQQQQAVALHQARLQTVRENLMSSDAFISDSAGNLIRTAIVSNDPVARQFAADVLAPMTSNNSSSSTSSSTTTNVLTDPSNATSTLPSSTATNTQPLATSSSTASTTATTHVGGSTSSVPPPLASDLRPSSQAHNHPGLQGLHPHPPLQPLLLLPLNPPPLPPSTSSSSSSTVTIMNTDPIPVPTPFSDPSLTHQLASNPSSSHPSIVRLPLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3JVD3</accession>
    <name>E3JVD3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phospho-2-dehydro-3-deoxyheptonate aldolase</fullName>
            <ecNumber evidence="6">2.5.1.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_01339</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>D-erythrose 4-phosphate + phosphoenolpyruvate + H2O = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14717"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="2.5.1.54"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the class-I DAHP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.54" evidence="6"/>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP76008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320427.1">
        <property type="nucleotide sequence ID" value="XM_003320379.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JVD3"/>
    <dbReference type="STRING" id="418459.E3JVD3"/>
    <dbReference type="GeneID" id="10533250"/>
    <dbReference type="KEGG" id="pgr:PGTG_01339"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01339"/>
    <dbReference type="eggNOG" id="ENOG502QPSU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030903_0_1_1"/>
    <dbReference type="InParanoid" id="E3JVD3"/>
    <dbReference type="OMA" id="MNGSFQI"/>
    <dbReference type="OrthoDB" id="4699125at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003849">
        <property type="term" value="F:3-deoxy-7-phosphoheptulonate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000005">
        <property type="entry name" value="Phospho-2-dehydro-3-deoxyheptonate aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006218">
        <property type="entry name" value="DAHP1/KDSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006219">
        <property type="entry name" value="DAHP_synth_1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00034">
        <property type="entry name" value="aroFGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009395">
        <property type="entry name" value="PRK12755.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009396">
        <property type="entry name" value="PRK12756.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225:SF20">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE DAHP SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00793">
        <property type="entry name" value="DAHP_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001361">
        <property type="entry name" value="DAHP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 6" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAHP synthetase I/KDSA" evidence="7">
        <location>
            <begin position="61"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR001361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP76008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="382" mass="41059" checksum="C2993CEF86238F58" modified="2011-01-11" version="1">MLKPELTHSRSHSGSSVVNVLDDLRVKNVRPLIPPQILMEDYPVSQTAINTITAGRLGADAIVKGQDDRLLVIVGPCSIHDTKAGLEYAKLLKAYADGAKDHLHILMRTYFEKPRTTVGWKGLINDPCLDGSFQINKGLRIARGFLLECAELGIPVAVEFLDQISPQYIADTVSWGAIGARTTESQCHRELASGLSTPVGFKNGTNGSVGIAIDAIRAAKSGHVFLSVTKQGISAIVETNGNDACHVILRGSNTGPNFSSSHIKECVQELSKAGLKPLVMVDCSHGNSEKKHERQLVVTEDLANQLENPETQDFIMGVMIESNLVEGKQSLEALGPNKLAYGQSITDACLDWESTKKALDRLKAAVGQRRSQKLAAGSSGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3JVW5</accession>
    <name>E3JVW5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Succinyl-CoA:3-ketoacid-coenzyme A transferase</fullName>
            <ecNumber evidence="3">2.8.3.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_02631</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>a 3-oxo acid + succinyl-CoA = a 3-oxoacyl-CoA + succinate</text>
            <dbReference type="Rhea" id="RHEA:24564"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:35973"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:90726"/>
            <dbReference type="EC" id="2.8.3.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Ketone metabolism; succinyl-CoA degradation; acetoacetyl-CoA from succinyl-CoA: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the 3-oxoacid CoA-transferase family.</text>
    </comment>
    <dbReference type="EC" id="2.8.3.5" evidence="3"/>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320609.1">
        <property type="nucleotide sequence ID" value="XM_003320561.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JVW5"/>
    <dbReference type="STRING" id="418459.E3JVW5"/>
    <dbReference type="GeneID" id="10533758"/>
    <dbReference type="KEGG" id="pgr:PGTG_02631"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02631"/>
    <dbReference type="eggNOG" id="KOG3822">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019942_1_1_1"/>
    <dbReference type="InParanoid" id="E3JVW5"/>
    <dbReference type="OMA" id="GMGPHPT"/>
    <dbReference type="OrthoDB" id="1933379at2759"/>
    <dbReference type="UniPathway" id="UPA00929">
        <property type="reaction ID" value="UER00894"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008410">
        <property type="term" value="F:CoA-transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008260">
        <property type="term" value="F:succinyl-CoA:3-oxo-acid CoA-transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046952">
        <property type="term" value="P:ketone body catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1080.10:FF:000001">
        <property type="entry name" value="Succinyl-coa:3-ketoacid-coenzyme a transferase subunit b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1080.10">
        <property type="entry name" value="Glutaconate Coenzyme A-transferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012791">
        <property type="entry name" value="3-oxoacid_CoA-transf_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014388">
        <property type="entry name" value="3-oxoacid_CoA-transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004165">
        <property type="entry name" value="CoA_trans_fam_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004164">
        <property type="entry name" value="CoA_transf_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02428">
        <property type="entry name" value="pcaJ_scoB_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13707:SF60">
        <property type="entry name" value="ACETATE COA-TRANSFERASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13707">
        <property type="entry name" value="KETOACID-COENZYME A TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01144">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000858">
        <property type="entry name" value="SCOT-t"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00882">
        <property type="entry name" value="CoA_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01274">
        <property type="entry name" value="COA_TRANSF_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="294"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="302"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="312"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="active site" description="5-glutamyl coenzyme A thioester intermediate" evidence="4">
        <location>
            <position position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR000858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR000858-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP76190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="541" mass="58056" checksum="1F28C5060F9F8E9A" modified="2011-01-11" version="1">MVYLKQSMASLHGSFHWKKGISLALSTRPILTSRFLSIAINQPKPSNGKLFRSADEAIVDIKSNSLIASSGFGLCGAPDTLIQAVARRPEIKNLTVISNNAGVDKAGLGLILHTRQVSKLIGSYIGANKLLENLYLTGQISLELTPQGTLAEKCRAGAAGIPAFYTPTGYGTPVQTGLIPIRYSAQDPSKVEIPGEKREVREFDGKQYLLERAIKADYAFVHVWKADRFGNCVFRYSAQNFGGVMARNAKVTIVEAEEIVEPGEIDPDQVHLPGIFVNRIVPATAKKQIEKIVLRSGSQDQSPTGSSNKSESSTKSDDPKVAAKLKRELIAGRAAQELQDGMYVNLGIGMPMLVPEFLKPGTNVHLQSENGIIGMGPYPSAEEVDGDIVNAGKETVTLLPGASTFDSSESFAMIRGGHVDVSILGAMQVSRSGDLANFMIPGKSVKGMGGAMDLVSNPDSTKVMIVMDHCDKHGVSKLVNDCQLPLTGTRCVSQVITDLAVFDIDRNAGKMTLVDLQPGVTLQEVKDKTDAEFEIGGKLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3JW79</accession>
    <name>E3JW79_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02745</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320723.1">
        <property type="nucleotide sequence ID" value="XM_003320675.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JW79"/>
    <dbReference type="GeneID" id="10534034"/>
    <dbReference type="KEGG" id="pgr:PGTG_02745"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02745"/>
    <dbReference type="HOGENOM" id="CLU_025212_0_1_1"/>
    <dbReference type="InParanoid" id="E3JW79"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033018">
        <property type="term" value="C:sarcoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051279">
        <property type="term" value="P:regulation of release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10033">
        <property type="entry name" value="CALSEQUESTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10033:SF0">
        <property type="entry name" value="CALSEQUESTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="448" mass="50206" checksum="2E66E99FDA88948C" modified="2011-01-11" version="1">MAATRSTQAHQTRSSSKRTSSAAKTQATHKKGHAVRKQASSSRKKAKSGKRQPNHTDENTDENTDEDDHHDTTPEPTATQQHRSSATESGQCNSGSNNIQLTLDNFESQLDNWSILQLRQTLNKNKDTRSNRIPPEVQDRLTLLQKNYTKSKLMLGLIGNISEAFSVESANTPVPPPGCSIGWEERNVHLGEAWALLSEEEKDVFSPVIFQYFSKIPCQPDTGDDDEDTEIELTPEEIELYQPLYAKLVNQEKVNLIISEGVHIGINNSKAFEQVERSVDKLNGELFTMSTLYNTTYYLLAATRDSGVKSFCKEWSNDVAWLALAKKNWKAKEKFEAYSHGREIQEAVEDASGVSTSKQLREADQLKKDLRAALNQTLANTLGIPVKGCIFPKVPNPSACLLKKNRRLEIVQSEGSTLPKEELMKGFNKMENPFKKTGLMISDLEHLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZJ1</accession>
    <name>E3JZJ1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03422</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77466.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321885.2">
        <property type="nucleotide sequence ID" value="XM_003321837.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZJ1"/>
    <dbReference type="GeneID" id="10538339"/>
    <dbReference type="KEGG" id="pgr:PGTG_03422"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03422"/>
    <dbReference type="HOGENOM" id="CLU_152097_0_0_1"/>
    <dbReference type="InParanoid" id="E3JZJ1"/>
    <dbReference type="OrthoDB" id="10268286at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171649" description="Secreted protein" evidence="1">
        <location>
            <begin position="25"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77466.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="146" mass="16345" checksum="12CE4E04407DAD81" modified="2012-04-18" version="2">MSTRVKNCTIICSAILQLFLPASAMEGAGGLVVISNCLYEGCRKKCHVLPDTLVRQYRFPLEGECGPYFDGQSWKHCHNIRRKIYFRCGTCARLHYTNWTMVRVVTGEDCWIKPHLTLDKVPIKPPELDLIAHFSGQGSSSAGPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3K0Z0</accession>
    <name>E3K0Z0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_03921</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP77965.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322384.2">
        <property type="nucleotide sequence ID" value="XM_003322336.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K0Z0"/>
    <dbReference type="GeneID" id="10541113"/>
    <dbReference type="KEGG" id="pgr:PGTG_03921"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03921"/>
    <dbReference type="HOGENOM" id="CLU_050263_0_0_1"/>
    <dbReference type="InParanoid" id="E3K0Z0"/>
    <dbReference type="OrthoDB" id="2516277at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="186"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="83"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP77965.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="347" mass="39234" checksum="7379B3E0F363B885" modified="2012-04-18" version="2">MTRTKRQSQLRAQQRMGPFSPGKPGALSGRMALDHQEFKGRRVVFPPAVSQSSTHPPPSPLALGDPGLLNGCMALDHQDYKGSSVVSSSIRSSENPEAPKSRNPPPKHASGTAKKIARLQKKLGCDDNVWILPTSISLKKINSDKPIQILPNLNRPRNLVTSSGGVPHFILERHPFDIKPPTHEFQELSNVIKTLYQMAQNRPTNTMNTKRLGGEMRMIGFRCASDRGKRGGTYVRNDLTPEEEAKDDQLWEKLQSHNDFLARRMESLCSSAYQENQSIMCDYGIPNWSQEEWSEMRKEEDVEYKFASNVSVTFDNFYNKAHQDEGDLNGWTYDTLISLTLYIRMVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3K246</accession>
    <name>E3K246_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04371</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322834.1">
        <property type="nucleotide sequence ID" value="XM_003322786.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K246"/>
    <dbReference type="GeneID" id="10536013"/>
    <dbReference type="KEGG" id="pgr:PGTG_04371"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04371"/>
    <dbReference type="HOGENOM" id="CLU_761028_0_0_1"/>
    <dbReference type="InParanoid" id="E3K246"/>
    <dbReference type="OMA" id="HERHITY"/>
    <dbReference type="OrthoDB" id="2501673at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="340"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="364" mass="41347" checksum="F2CDFB1DBA785599" modified="2011-01-11" version="1">MKSIIKRIKRRASPTTASALLVPPVTTQTQHLPKHIRASSHEQSVRTRETFVTPSNNSSSQLSPDLAVPNNSRIGQSTSQDYKSPESATQVNPSHHIPENMESFISITDSSASGERSYTTRVSANSEERLSSHLPQGEEAQLESAKNDGLRSPSVNAPSIRTFGDHAHRQTQSNFVHVVPDDSERDSTTTYYEEKHTLKPVTRETIHKHVVEEVQKVKLHERHLTYVQHHVQPIINPSHEIEFQDYRIELPDQPGPQIDRINVEKKLKLDTIIDDLIRNNKKRYERDGHQKVTNSQSIELPDVEHQAMVVHNYHIIQPVILNPERVPEETVHLKLNKQTVVSSATEAKSTTLEDTYPQSPWHNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2I0</accession>
    <name>E3K2I0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_04505</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322968.1">
        <property type="nucleotide sequence ID" value="XM_003322920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2I0"/>
    <dbReference type="GeneID" id="10536313"/>
    <dbReference type="KEGG" id="pgr:PGTG_04505"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04505"/>
    <dbReference type="HOGENOM" id="CLU_678155_0_0_1"/>
    <dbReference type="InParanoid" id="E3K2I0"/>
    <dbReference type="OMA" id="QIYTHES"/>
    <dbReference type="OrthoDB" id="2508554at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP78549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="406" mass="45578" checksum="1DFFB683D590C61C" modified="2011-01-11" version="1">MAAHERSGTISYEELCEIYSKEIIDQATRPSIEDDEDVPVDPVNPAPAEESVLLQLTYSVWLSNPPATEATHGPRSQRRSYLWHEVHGPPTRPLYICIPPAAREWSWGGFQRGVIRSLVSHNLEVGHEIRCNHAAGELVWQMSRGSAAQPSPDPALSLICGRSWPASVRRILRTPHRFRLHIMAPDGPWQLDPEPSINRQPTPPHDVGPGRLDWTYLHCPTPSSGPRAPPMQPRTPPPGEIIVPRQVYVDYTLYVPIGRDPHGVIYTQIYQGKDHPSAPMSLLLQGLTPAKLTRLVFFHLSRERRSIHVDRVAQDADATKSLQWHFTIIEPGQAPALGSFMRGEGCFEDFVNAVGASSPLAKVVIRLYMQYDDRHLRPLPPKERRYLVIGPRSPRDDTTSRTQGTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>E3K4D6</accession>
    <name>E3K4D6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_05735</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79414.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323833.2">
        <property type="nucleotide sequence ID" value="XM_003323785.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K4D6"/>
    <dbReference type="FunCoup" id="E3K4D6">
        <property type="interactions" value="852"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K4D6"/>
    <dbReference type="GeneID" id="10531238"/>
    <dbReference type="KEGG" id="pgr:PGTG_05735"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05735"/>
    <dbReference type="HOGENOM" id="CLU_011751_0_1_1"/>
    <dbReference type="InParanoid" id="E3K4D6"/>
    <dbReference type="OrthoDB" id="10006270at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005680">
        <property type="term" value="C:anaphase-promoting complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031145">
        <property type="term" value="P:anaphase-promoting complex-dependent catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045842">
        <property type="term" value="P:positive regulation of mitotic metaphase/anaphase transition"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF9">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 16 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="1" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0498">Mitosis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7" id="KW-0802">TPR repeat</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="546"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="7">
        <location>
            <begin position="580"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="25"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="148"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="630"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="33"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="160"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="188"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="630"/>
            <end position="639"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP79414.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="704" mass="79532" checksum="04797B9B415B9E49" modified="2012-04-18" version="2">MNSNNTPPINRLKHLLINKLEPLSSTLNIHPKTQQQQQQQQQQQQQQQQQQQQNSFSSTNNQQSPSSQSESEPEESQPPTCFWDELLPDDSSLDPNNTTTLSEGEPEEGKTVLVRMTDYSTACRYLAAQCMIRQGKWDAALEMVGEENPFRSPLSGQQEHDDDDDDDEHLDLSILNPPSTTIPPEKPPPPEKQQDPTGKKPNDRHAQTRGRAAAAAMDGGIKFESSLCYLRGLIHLNHKALDRAKQAFLESLALDVKCYESFEALVGGNMLEPEEEWEFIQSLEYHSQTPEDALFIRSLYTVRLKKFEHKTHLFESLKVLKNQYGLSDDPDVAFGLADWLYTNYRFIDAYRKLMFVIVGILKLHYHHLPTLPLHLSAMTMIKKLLPALFLLAHELIDREPCSPISWYAAGLWYFSQKRWEESRRFFSKAALLDSRFPEAWFAFGHALAYEGEHDQAITAYSTASHNFQGSHLPLLFIGMQHIQLANPVLANDYLSAAAEISPCDPLVMHERGVVAYYQEQWESAVDLFNQTLTLVLKAQTDPLIWAPTYLNLAHCYRRLKRFAESLEAAEKAKALLPRSASVLSALGMAHLGLGQNLEAIKYYHESLAVLPADPMTTCLLQFSLDLETTTNATTSTTPRTRIRTGEEGPGEGEAGGPELGLVAQIVPRQIRQSWLDDFRLFDHKLEQQYGDQVDAAALLHPPGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3K4Q4</accession>
    <name>E3K4Q4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_05541</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323639.1">
        <property type="nucleotide sequence ID" value="XM_003323591.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K4Q4"/>
    <dbReference type="GeneID" id="10531044"/>
    <dbReference type="KEGG" id="pgr:PGTG_05541"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05541"/>
    <dbReference type="HOGENOM" id="CLU_1826240_0_0_1"/>
    <dbReference type="InParanoid" id="E3K4Q4"/>
    <dbReference type="OrthoDB" id="2510295at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="106"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="21"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP79220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="141" mass="16630" checksum="8D0D3CA1F4F54474" modified="2011-01-11" version="1">MQKAQEATASWAAEERRDALVEQAQQDALRVEERANRQRLEDAQADKRAAELKCQDELRADDRWAGEKRADAMEVPRRQEMQEAKEDRELLCQQAKLDREVANHQMKMEREAAQARAAEDWKRERQRDLDQKAKEKKEAQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3K5B2</accession>
    <name>E3K5B2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_06029</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79708.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324127.2">
        <property type="nucleotide sequence ID" value="XM_003324079.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K5B2"/>
    <dbReference type="GeneID" id="10533069"/>
    <dbReference type="KEGG" id="pgr:PGTG_06029"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06029"/>
    <dbReference type="HOGENOM" id="CLU_2085940_0_0_1"/>
    <dbReference type="InParanoid" id="E3K5B2"/>
    <dbReference type="OrthoDB" id="10278993at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP79708.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="122" mass="13947" checksum="9DF90CE79CF662E1" modified="2012-04-18" version="2">MPPNSEGLQQFAEEDVSLVRRIGKLMCHRLADTDRTPPSRTKPCHLAENISLPSWYGRYQLEDEDSSRSAETVLSQFAEKEIRWTTADGVIKLGAATPKNYRHGLWRRFIVNMCGILLQKES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3K5R5</accession>
    <name>E3K5R5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Importin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_05807</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79486.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323905.2">
        <property type="nucleotide sequence ID" value="XM_003323857.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K5R5"/>
    <dbReference type="FunCoup" id="E3K5R5">
        <property type="interactions" value="1008"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K5R5"/>
    <dbReference type="GeneID" id="10531314"/>
    <dbReference type="KEGG" id="pgr:PGTG_05807"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05807"/>
    <dbReference type="eggNOG" id="KOG2023">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008136_1_0_1"/>
    <dbReference type="InParanoid" id="E3K5R5"/>
    <dbReference type="OrthoDB" id="951172at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061608">
        <property type="term" value="F:nuclear import signal receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008139">
        <property type="term" value="F:nuclear localization sequence binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.10.10:FF:000313">
        <property type="entry name" value="Importin beta-2 subunit, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001494">
        <property type="entry name" value="Importin-beta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040122">
        <property type="entry name" value="Importin_beta"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10527">
        <property type="entry name" value="IMPORTIN BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13513">
        <property type="entry name" value="HEAT_EZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03810">
        <property type="entry name" value="IBN_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Importin N-terminal" evidence="7">
        <location>
            <begin position="33"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="82"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="377"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="383"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="412"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP79486.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="968" mass="106026" checksum="E2CB48981CED83F9" modified="2012-04-18" version="2">MSNWIPQPDGLAQLLTCLRNSDSADTTIQQSVTKHLDSFNEVPDYNSYLAYILSSMPQEDARVRSVAGLILKNNLRRLPYPNPILLQDPTRTNTTTSNQNSSNPPSLSGSQQTLEYVKSSVLSSGLLSDPVPMLRATAGTLISTIVMQFGPESWPQALQTLIELTEANEPLGKEGAFSTLSKICEDVPRKLEHMQINGTRVLDIMIPRLINHLKNPSDSRIRAYALICLNPFIQTGGDGSLTDNLQSYVEALFLSASDRSPEVRKNVCSALVSLLSSNPDVLIPNLSQTVDFMLYSTQETDEGVALEACEFWLAFGEDIRLKNYLAGYLEKIVPVLLKGMIYSEEDLLILDNDEEDEAVPDRQQDIKPRFYGAKDARTHTDDSAAASPSTTHPASNIDPNQSDDRQNGAATGEDDNEESELDEDGDSDEDDIYAEWNLRKCSAAALDVIAVNFENSLLEYLLPILKEYLFQPKWEHKEAAILALGAIAEGCLTGMEPHLSTLIPILLDCLKDRKALVRSITCWTLGRYSSWIISPGSTTAEHKQAVFLPVMEGNKRVQEAGCSAFATLEEEAGEELEPYLLPILSSLVTAFRKYQHKNLLILYDALGTLADSVGSSLAKPELLQVLMPSLIERWQKLADDEQDLIPLLECLSSVVIAIGPAFGPYAPAVFERCVRLVANNLEVYALSAKHPGEYEPPDKTFLIVALDLLSGLTQGLAQGMNAMSNPTNNPSNRHALPPILSLLGSCLSAEFPEPSVRQSAFALVGDCAISCYPLLESYLPQFMPQIIRQIDAEAPITLTSVCNNAAWAAGEISIKAGEKMAGFIEPLLSRLVPILNSTRVARSLTENAAVTIGRLSLSCPHLIAPHLDQFIRQWCQALAEIKDNDEKDSAFRGMCLAIEIYPHGLIRGVSASGSTPSADGADRLPNLSLKFKSLLHAFKNAMGENQWAVTLAALTPQTAQRLQVAYQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3K6K0</accession>
    <name>E3K6K0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05122</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EFP79897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324316.1">
        <property type="nucleotide sequence ID" value="XM_003324268.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K6K0"/>
    <dbReference type="GeneID" id="10533588"/>
    <dbReference type="KEGG" id="pgr:PGTG_05122"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05122"/>
    <dbReference type="HOGENOM" id="CLU_045924_0_0_1"/>
    <dbReference type="InParanoid" id="E3K6K0"/>
    <dbReference type="OrthoDB" id="10282771at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="286"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="355" mass="39420" checksum="1D3DA22BC4983B40" modified="2011-01-11" version="1">MSHPAEDQATEALPTAIIPVERRLRYPIIYLSYHVWVEHRVLFPDGTPSYARRSEAPRTPENWRYHHECITTTYDEVAPIPPCKVPFDHLSLRDFKRGVIELIGSTRSDFDFVGVLRAADSANKLQWRGYRTWPGGPTPRNMNVRYHFRDFARMATRAVLPMKIHLKLFMDDPAQIVGNRRYWTWIPHIWPHEVIAPFVPNGKLPPPPEDCLVLGGQLPLPHAGEQATTSRLSVDTPASPFRNADDDPTTTQLSMGPAPSLSSWVIETPSSTTPTPPLVQSPSVHSSSARARSSSYFEPPCSSDSDSDSNEPEIDAPLSSPVATEDVESTAVNAPPSSPHILVTDERVNSGPSGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3K755</accession>
    <name>E3K755_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="6">40S ribosomal protein S5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_05210</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
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    <dbReference type="KEGG" id="pgr:PGTG_05210"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05210"/>
    <dbReference type="eggNOG" id="KOG3291">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_063975_0_0_1"/>
    <dbReference type="InParanoid" id="E3K755"/>
    <dbReference type="OMA" id="KMNIVER"/>
    <dbReference type="OrthoDB" id="10264639at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0003735">
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    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000054">
        <property type="term" value="P:ribosomal subunit export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14867">
        <property type="entry name" value="uS7_Eukaryote"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.455.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="entry name" value="Ribosomal protein S7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_uS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020606">
        <property type="entry name" value="Ribosomal_uS7_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_uS7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_uS7_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005716">
        <property type="entry name" value="Ribosomal_uS7_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003106">
        <property type="entry name" value="PRK04027.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01028">
        <property type="entry name" value="uS7_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="RIBOSOMAL_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS7" evidence="5">
        <location>
            <begin position="45"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP79985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="204" mass="22657" checksum="23188C4DA7658AE0" modified="2011-01-11" version="1">MANCTVLPTEVLAANESGINLFGRWDSADVEVKDICLTDYIQIRNPVYVPHTAGRYATKQFRKAQMPIVERLVNSLMMKGRNNGKKQMAVRIVAHAFEIIHLLTDQNPIQILVDAIVNTGPREDSTRIGSQGTVRRQAVDVSPLRRVNQAIALLTIGVRESAFRNVKSISECLADELINAAKGSSNSYAIKKKDELERVAKSNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3K7H3</accession>
    <name>E3K7H3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF3295 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_06433</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324896.1">
        <property type="nucleotide sequence ID" value="XM_003324848.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K7H3"/>
    <dbReference type="STRING" id="418459.E3K7H3"/>
    <dbReference type="GeneID" id="10539488"/>
    <dbReference type="KEGG" id="pgr:PGTG_06433"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06433"/>
    <dbReference type="HOGENOM" id="CLU_987440_0_0_1"/>
    <dbReference type="InParanoid" id="E3K7H3"/>
    <dbReference type="OrthoDB" id="2507811at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021711">
        <property type="entry name" value="DUF3295"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053043">
        <property type="entry name" value="Ras-cAMP_regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28014">
        <property type="entry name" value="NEGATIVE REGULATOR OF RAS-CAMP PATHWAY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28014:SF1">
        <property type="entry name" value="NEGATIVE REGULATOR OF RAS-CAMP PATHWAY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11702">
        <property type="entry name" value="DUF3295"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF3295" evidence="2">
        <location>
            <begin position="134"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="193"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="24"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="201"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP80477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="282" mass="30614" checksum="B2F12DBA1EC2E5E6" modified="2011-01-11" version="1">MVQRSPSSLSGNSSLRHRPPTRRNSAAPTAGPSTTANPQPPTRSLPHPAHQRHPSRLGGKPENIEYSEDSESNSEEGEGGSGGRDEERGKRAEQKRADQERRLGRLERRTGTNTEPIALGNSALLLANRPEPVAPPLSPRTTRRNMLANEMTESVRRNLLWERQIQGLALGVVPSRVEVNRSESLALGFRHHQHASNLPNSSTTTSTADPSTSSSNNPIANPNQNPNNNNNNTGTTTATTTSTAAAAAATTSTQFKKTHLASLTNNNKEYRNFSKGFHRTGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3K7Q4</accession>
    <name>E3K7Q4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06308</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80352.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003324723.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K7Q4"/>
    <dbReference type="STRING" id="418459.E3K7Q4"/>
    <dbReference type="GeneID" id="10539360"/>
    <dbReference type="KEGG" id="pgr:PGTG_06308"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06308"/>
    <dbReference type="HOGENOM" id="CLU_2062629_0_0_1"/>
    <dbReference type="InParanoid" id="E3K7Q4"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP80352.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="119" mass="12875" checksum="47181EA0DE39549C" modified="2012-04-18" version="2">MYARPPPPAISEKVTEEPKKAPLKSTTQKSKPAIIDIDDDEIEVVKPGQTTQSLQTATTSRKRSGEYSNTLQTATTSRKRSGDYNNNATSLSSGGPSTSVAPESNPQAKRSRKQVIELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3K8Y0</accession>
    <name>E3K8Y0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_06618</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325081.1">
        <property type="nucleotide sequence ID" value="XM_003325033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K8Y0"/>
    <dbReference type="GeneID" id="10539678"/>
    <dbReference type="KEGG" id="pgr:PGTG_06618"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06618"/>
    <dbReference type="HOGENOM" id="CLU_410569_0_0_1"/>
    <dbReference type="InParanoid" id="E3K8Y0"/>
    <dbReference type="OrthoDB" id="2508220at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP80662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="640" mass="73517" checksum="9D5C1D1BCCFF20A2" modified="2011-01-11" version="1">MDQPLRCYFMHLFIWIMTAVSGTILPNLKPKVLEEANSGCEDIPHPLTRSQVLLDTISPCLTETFIPAHSLNPPERSYPHALSRSRLPDHAGPSSSPFPHPISTTHVFQEIPASSQAPAAKRKCQQISMNSSPSSSSTTSTSSDNSIRKRPKQEQFDPFELVNGKRAGYSSLNHLDAGQLATEQRGPKRGSYVLKLNRPKQPNSLYFTPGLTYLSFIKKAYDRFEAKLASLTLDVTQLRNKLQHPLEMINMHWSFDRPDKVVRVKHSSTEAVQSFTNWKTLYRVLIGWIYERHEKLMNNFNLPTYVQRFQHEKLISWLDQQIFSPPTGLPIMGRIDVPNELSWRNHRFPLIQLQLIKYFAHDVDMNLLAPTTALSVIAAFQTQYISDYVASRYFLEASTRISTSAEFQMLRSFLINSLGPEQRQTLGHHGSFGTKDPIFQSTFKRFHDNLTATSLRKLEVKSFHPKLPIAMYFVDKKSDRLPFRVLSRDDRSAIKNRVFNSMFKRLAKAMKIFHLKILSLLDIKTPETIRTRTEEMLQWLLKSIIEPKDSLCVIGHARINGDFAPWEDITNGALNLYGQAQLELIDYFSTRFSNPILESSAVFILCLWYKYYHPKEFNYLAAQSHEHHSSLATQEANQNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3K953</accession>
    <name>E3K953_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_06645</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80689.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325108.2">
        <property type="nucleotide sequence ID" value="XM_003325060.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K953"/>
    <dbReference type="GeneID" id="10539707"/>
    <dbReference type="KEGG" id="pgr:PGTG_06645"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06645"/>
    <dbReference type="HOGENOM" id="CLU_133971_0_0_1"/>
    <dbReference type="InParanoid" id="E3K953"/>
    <dbReference type="OrthoDB" id="2504051at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP80689.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="176" mass="20156" checksum="C408C02B30AAF5CA" modified="2012-04-18" version="2">MVKHERTHLRNLLLTHVRQDARPRELGPIPKIFDLIILIDRSFQPRNTLRSAAEIRGSLTSDQMVQFAMIRLLTIHHHLHRAPGDTRSQWDVIDDQFEAIREKSNVQLHALSILILRCDRALFPGDFMFADIPEEIIRMPSVLDCEIKTRAIVAELGEEPTDEALARLVDSEAGLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>E3KA27</accession>
    <name>E3KA27_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_07261</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP81009.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325428.2">
        <property type="nucleotide sequence ID" value="XM_003325380.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KA27"/>
    <dbReference type="GeneID" id="10536248"/>
    <dbReference type="KEGG" id="pgr:PGTG_07261"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07261"/>
    <dbReference type="HOGENOM" id="CLU_2005016_0_0_1"/>
    <dbReference type="InParanoid" id="E3KA27"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP81009.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="124" mass="13653" checksum="33DEA81BD0AF1A75" modified="2012-04-18" version="2">MLHLPKILFLRPGATRTRGCFRDRWHLAKTAVLPTGGPSRKGLLLRPDSGGTSRKSFFLQLDASRERASFRDRWVALQPGPPRERGSFGDLGHLPQQALLATGGTSPASQSAKRASFWRGAPFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCM0</accession>
    <name>E3KCM0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_07569</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82172.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326591.2">
        <property type="nucleotide sequence ID" value="XM_003326543.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCM0"/>
    <dbReference type="GeneID" id="10529544"/>
    <dbReference type="KEGG" id="pgr:PGTG_07569"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07569"/>
    <dbReference type="HOGENOM" id="CLU_047039_0_0_1"/>
    <dbReference type="InParanoid" id="E3KCM0"/>
    <dbReference type="OrthoDB" id="2505895at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="47"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="196"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="277"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="56"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP82172.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="345" mass="38956" checksum="2BC12259A13BFA36" modified="2012-04-18" version="2">MSLMFNRKSASFIEIEEKLREVEARIIADRKRREEELKTFVEQARRDAEAIRKTKSQQNPPNPSDTTGPQLQKPLASPLIPNISSSPRITRELPETSLFLLRDLWSHVGYSGESFVEMQMEISRELDRADFARFEREMICNEVIGTRAEINMLRDERTQLLKRLDLNRENTCQLIETQERTYKTLMSERVTSANVIRGLENEIRDRNAANQGARDRIATLSNELERAEQTRIATVLCAKGAEDRASVANATALAAVQVANQQREHVPPTNPTTPSTAQGNEALKNKLKEQILAVEEEKIASQLASVQEEREKLKNQTKGPSNAAAENKSPLLSAISQLVHNGLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDD9</accession>
    <name>E3KDD9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cysteine synthase 1</fullName>
            <ecNumber evidence="5">2.5.1.47</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">O-acetylserine (thiol)-lyase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="17">O-acetylserine sulfhydrylase 1</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="18">O-succinylserine sulfhydrylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PGTG_07545</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the conversion of O-succinyl-L-serine into cysteine, the last step in the cysteine biosynthesis pathway. Can also use O-acetyl-L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate</text>
            <dbReference type="Rhea" id="RHEA:14829"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:58340"/>
            <dbReference type="EC" id="2.5.1.47"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>O-succinyl-L-serine + hydrogen sulfide = L-cysteine + succinate</text>
            <dbReference type="Rhea" id="RHEA:53816"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:136856"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the cysteine synthase/cystathionine beta-synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.47" evidence="5"/>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82148.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326567.2">
        <property type="nucleotide sequence ID" value="XM_003326519.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDD9"/>
    <dbReference type="FunCoup" id="E3KDD9">
        <property type="interactions" value="378"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KDD9"/>
    <dbReference type="GeneID" id="10534680"/>
    <dbReference type="KEGG" id="pgr:PGTG_07545"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07545"/>
    <dbReference type="eggNOG" id="KOG1481">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021018_1_0_1"/>
    <dbReference type="InParanoid" id="E3KDD9"/>
    <dbReference type="OrthoDB" id="10259545at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004124">
        <property type="term" value="F:cysteine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141223">
        <property type="term" value="F:cysteine synthase activity, acting on O-succinyl-L-serine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006535">
        <property type="term" value="P:cysteine biosynthetic process from serine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01561">
        <property type="entry name" value="CBS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000011">
        <property type="entry name" value="Cysteine synthase (o-acetylserine)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050214">
        <property type="entry name" value="Cys_Synth/Cystath_Beta-Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001216">
        <property type="entry name" value="P-phosphate_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007989">
        <property type="entry name" value="PRK10717.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10314">
        <property type="entry name" value="CYSTATHIONINE BETA-SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00901">
        <property type="entry name" value="CYS_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0198">Cysteine biosynthesis</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="19">
        <location>
            <begin position="54"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00050981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00058228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00072087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00078262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00079147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00081847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="EFP82148.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="412" mass="45185" checksum="68E14F07C7DAED30" modified="2012-04-18" version="2">MSRRLISRSNGLVRVAPENIHRGLTLPIRRSERVDRKAYGSISSAYSQPVDGFTGAIGNTPLIHLKRLSEQTGCEIFGKAEFLNPGGSVKDRAALWIIKDAEEKGLLKRGGTVVEGTAGNTGIGLAHLCRSRGYRCIIYMPNTQSPEKINVLKKLGAEVYPVPAVPFDDPQNYNHQAARCAESMENAVWTNQFDNLANRQAHIETTGPEIWEQTLPVGGIDGFICSTGTGGTLAGVTRYLKSKSDGKVKCYLADPPGSVLYNYIQSNGKITDKREGNGGSVTEGIGQGRITNNLAPEIRDNLIDGSFYIPDQDSIKLFFELIDLDGFSLGLSSALNLLSTVKLAEKLKQEQQLASQQSNHPDGHPQKKLNLVTILCDYSTNYQSKLFSKSWLKSKGLFDSIPDHLRQYAILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDF2</accession>
    <name>E3KDF2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_08344</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82388.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326807.2">
        <property type="nucleotide sequence ID" value="XM_003326759.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDF2"/>
    <dbReference type="GeneID" id="10529765"/>
    <dbReference type="KEGG" id="pgr:PGTG_08344"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08344"/>
    <dbReference type="HOGENOM" id="CLU_1210335_0_0_1"/>
    <dbReference type="InParanoid" id="E3KDF2"/>
    <dbReference type="OrthoDB" id="2505948at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045469">
        <property type="entry name" value="Nis1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19271">
        <property type="entry name" value="Nis1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173249" evidence="2">
        <location>
            <begin position="21"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP82388.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="229" mass="23471" checksum="E5E9F56E3EC5683E" modified="2012-04-18" version="2">MRVTTISLFGFLLSPLGSLAQDISIASPTKDSNVTPGQKLKIVLQAQGTTSSVKHIAFSTGFRATSKHDDKSLGRPTVGSVSPGSPDVAFDASKGTYTYEITVPAAKDFLDGFSAPYELTVSDLYLLGATFTPTLKLTSVQVNVQNNNSTGGDSPPADPNNANNSTNPTPNSTDPNGGKGSNSSPTPSSDPNSGSSSNSNKPSSGAASHVHPSAMYSACFLLAACLLAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDL5</accession>
    <name>E3KDL5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Putative nuclease HARBI1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Harbinger transposase-derived nuclease</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGTG_08407</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Transposase-derived protein that may have nuclease activity. Does not have transposase activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HARBI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82451.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326870.2">
        <property type="nucleotide sequence ID" value="XM_003326822.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDL5"/>
    <dbReference type="GeneID" id="10529830"/>
    <dbReference type="KEGG" id="pgr:PGTG_08407"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08407"/>
    <dbReference type="HOGENOM" id="CLU_018552_2_0_1"/>
    <dbReference type="InParanoid" id="E3KDL5"/>
    <dbReference type="OrthoDB" id="2502344at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026103">
        <property type="entry name" value="HARBI1_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF85">
        <property type="entry name" value="GH03217P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02086">
        <property type="entry name" value="PUTNUCHARBI1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="14">
        <location>
            <begin position="203"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="372"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="385"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EFP82451.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="418" mass="47692" checksum="21EB3564D8933C1D" modified="2012-04-18" version="2">MAYHHNEATEAILDGVMKLPSIPSFGAILFPGKPSLQFIWEMFFDDEAEVAEILEELLSHRYLSLRTTPTTHDDYDLAKLFDMRDNDFKQAVRTTKDGFLWLLDRIRHHPIFYSNSPRPQLPIPHQLALTLERLGSNGNGASVGRFSRNLSVGRGTVIKVSRRVIQAINEVSTNYIVWPNKERRAEISEVMTEEGFEGCIGFVDGTTIPLHQRPGLDGEVYWDRKKQYSINCQVICDCDRFITSFMTGWPGTCGDSLVFKNMKVHLEPDRFFDPGQYLIADSAYALSMTTIPAYKSPLSKINRNTEFNYCLAKSRVRNEHTIGILKGRWASLQQLRLSLYTPQHMVEIIRWINCCVALHNMLAHLGDSWDELATPNDNDEPGDSEGNHENNTEGHREEIQEKCLLFNHLVGTLPIPSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDM7</accession>
    <name>E3KDM7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Zinc-ribbon 15 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08419</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82463.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326882.2">
        <property type="nucleotide sequence ID" value="XM_003326834.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDM7"/>
    <dbReference type="STRING" id="418459.E3KDM7"/>
    <dbReference type="GeneID" id="10529842"/>
    <dbReference type="KEGG" id="pgr:PGTG_08419"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08419"/>
    <dbReference type="HOGENOM" id="CLU_115926_2_1_1"/>
    <dbReference type="InParanoid" id="E3KDM7"/>
    <dbReference type="OrthoDB" id="5545479at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28139">
        <property type="entry name" value="UPF0768 PROTEIN YBL029C-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28139:SF1">
        <property type="entry name" value="UPF0768 PROTEIN YBL029C-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="96"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82463.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="14344" checksum="8DAA11143BFAFB39" modified="2012-04-18" version="2">MCSDIPIPICISVGCPVKVTSGAPDGPGRACPKCHNGSVFKSHRRRWIEICFVSLIPCSSRQVWHCNICNWQANVDDSFQPAFPAPPPPPPGAGAGAAQPQMVVPQMQMIPPTSFAPPSGPPPPTELQYPGGYGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3KE36</accession>
    <name>E3KE36_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08786</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327009.1">
        <property type="nucleotide sequence ID" value="XM_003326961.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KE36"/>
    <dbReference type="GeneID" id="10529973"/>
    <dbReference type="KEGG" id="pgr:PGTG_08786"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08786"/>
    <dbReference type="eggNOG" id="ENOG502S284">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_341041_0_0_1"/>
    <dbReference type="InParanoid" id="E3KE36"/>
    <dbReference type="OMA" id="EAWESTF"/>
    <dbReference type="OrthoDB" id="3353982at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="368"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="471"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="550"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="413"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="484"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="573"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="599"/>
            <end position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="834" mass="94679" checksum="6D040B5B2C3AF8AF" modified="2011-01-11" version="1">MEKLPTEILELILQQIPNSALETTTKNLRKTLPNNDSNDLLLLQHQFKHLTITNQEELSQLIRAADDPDHQPLIKNFTQSISSLAWRYRDNNLLVNLINQIAKASDSLRILRLRIGPLFAPEQLDELFLSNLPTLQFIDLRFNQNLSRRSYEPFLKGAYFDSSIDLISKWPETRTFRSLSFVQDPPPQREEGAYSNNRGIAQPIILFRFWCLTKLSTSAIGQHLRHLRVRIPGRNVATSFLDNPEASASDESIRVGRQTCKLPRPPFPSLKLLDLSTTILLPSSISVTLVGALLRRLPSLTHLLLDRSQLLIPPRFLADVERVKDELHNLGSVVAAAGVPRANEAQKVWNRLYKCYETELEALLVPPSRFASQPDTPPAPPILNPSTSSSSTTTNGTRRKGRSAYASAPRWKSTPTTAQTSTSTGSTTPASPFDSCSKRSIRRKLIAQRMPDKVTILPGLSNLISLSCGTDLPSNDPFDPDDQNAQHDQETGEQEEKQGYEERTRSEWKECFETGWALGCRKVQDALTEKLLSHSRALLVWEKKLEKIRRDGNRPKTQTQMKSIIKTKTRTRKKEDEAKGKQKEKPTKSKTNNQVGKENEDDDDEDRDSEDEDQDSEEDSSEEESHEQIVRRCEANRPVLLIHETDPLRGLLVRPTDTDRCLDLVEEEDEEAHTSIFGHFLRTQHLVEVTQTRNGQFLEESEMGVQIGEKATRPPKFCSINDCDEVGRVVYDDDRGWSGARVNLFDFNNPFARPDRPHEPLLHHLSLAHKEALQNSPVWPRFHNLALSRLPLDQLDALAPLDHHLLQDSHLPASSHPLNCGHLVARKIWDIDSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>E3KFI6</accession>
    <name>E3KFI6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">NADPH--cytochrome P450 reductase</fullName>
            <shortName evidence="19">CPR</shortName>
            <shortName evidence="19">P450R</shortName>
            <ecNumber evidence="19">1.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">PGTG_09014</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="23" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="19">This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Involved in ergosterol biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="19">
            <text>2 oxidized [cytochrome P450] + NADPH = 2 reduced [cytochrome P450] + NADP(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24040"/>
            <dbReference type="Rhea" id="RHEA-COMP:14627"/>
            <dbReference type="Rhea" id="RHEA-COMP:14628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:55376"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="EC" id="1.6.2.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="19">Binds 1 FAD per monomer.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="19">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="19">Binds 1 FMN per monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="19">Endoplasmic reticulum membrane</location>
            <topology evidence="19">Single-pass membrane protein</topology>
            <orientation evidence="19">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Mitochondrion outer membrane</location>
            <topology evidence="19">Single-pass membrane protein</topology>
            <orientation evidence="19">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="19">Cell membrane</location>
            <topology evidence="19">Single-pass membrane protein</topology>
            <orientation evidence="19">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the NADPH--cytochrome P450 reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="19">In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="19">In the N-terminal section; belongs to the flavodoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="1.6.2.4" evidence="19"/>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EFP83061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327480.1">
        <property type="nucleotide sequence ID" value="XM_003327432.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KFI6"/>
    <dbReference type="FunCoup" id="E3KFI6">
        <property type="interactions" value="504"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KFI6"/>
    <dbReference type="GeneID" id="10544773"/>
    <dbReference type="KEGG" id="pgr:PGTG_09014"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09014"/>
    <dbReference type="eggNOG" id="KOG1158">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_17_3_1"/>
    <dbReference type="InParanoid" id="E3KFI6"/>
    <dbReference type="OMA" id="MTPTRIH"/>
    <dbReference type="OrthoDB" id="1856718at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003958">
        <property type="term" value="F:NADPH-hemoprotein reductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000100">
        <property type="entry name" value="NADPH--cytochrome P450 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000109">
        <property type="entry name" value="NADPH--cytochrome P450 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.360:FF:000024">
        <property type="entry name" value="NADPH--cytochrome P450 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.80:FF:000018">
        <property type="entry name" value="NADPH--cytochrome P450 reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.990.10">
        <property type="entry name" value="NADPH-cytochrome p450 Reductase, Chain A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03212">
        <property type="entry name" value="NCPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003097">
        <property type="entry name" value="CysJ-like_FAD-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001094">
        <property type="entry name" value="Flavdoxin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023173">
        <property type="entry name" value="NADPH_Cyt_P450_Rdtase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001433">
        <property type="entry name" value="OxRdtase_FAD/NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023208">
        <property type="entry name" value="P450R"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384:SF17">
        <property type="entry name" value="NADPH--CYTOCHROME P450 REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19384">
        <property type="entry name" value="NITRIC OXIDE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00667">
        <property type="entry name" value="FAD_binding_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00175">
        <property type="entry name" value="NAD_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000208">
        <property type="entry name" value="P450R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00369">
        <property type="entry name" value="FLAVODOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 19" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 19" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 19" id="KW-0274">FAD</keyword>
    <keyword evidence="3 19" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4 19" id="KW-0288">FMN</keyword>
    <keyword evidence="2 19" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="14 19" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="16 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="15 19" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 19" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="9 19" id="KW-0521">NADP</keyword>
    <keyword evidence="12 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 19" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="18 19" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="13 19" id="KW-0756">Sterol biosynthesis</keyword>
    <keyword evidence="17 19" id="KW-1207">Sterol metabolism</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="20">
        <location>
            <begin position="82"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="21">
        <location>
            <begin position="288"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="88"/>
            <end position="93"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="139"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="483"/>
            <end position="486"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="501"/>
            <end position="503"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="507"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="595"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="674"/>
            <end position="675"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <begin position="683"/>
            <end position="687"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="19">
        <location>
            <position position="755"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="EFP83061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="756" mass="84680" checksum="FBF2D971B13EF6D5" modified="2011-01-11" version="1">MLSLSSTDLILLSVIGALTLYWLYARLFANPSSSTVHRTNPTHQQQLQGFNIHNGPRSDSNPELLAAGRDFVKKMDLQKKKAVVFYGSQTGTAEDYATRIAKEAKSKFGISSLVADPDEYDFDCLEKLRADQLAIFVLATYGEGEPTDNAVNMLEQLKEQQTEGNQLDHLNYVVFALGNRTYEQFCQMGIIVDELLTKRGAKRIGELGMGDDDKSMEEDYLAWKDPMWEQVQKVMGWEEGAGGDEPDFLVKEVEVNPGKEGSIDDGQVYRGELSGRALMGTRGIFDAKNPCPARVANARELFAAGDRNCVHMEFEIQGTGIRYQTGDHLAVWPVNPDSEVARLMKLLGLWEKRATAVEISSLDPMLAKVPFPTPASYEAIFRHYLDISCIASRQLLSGLARFAPTEAASAALKAIGTDKEHYQRVVAGPGLRLGECLMSVLPGGAEKLSVEDPLSESFGQGWFQTYQIPFDQIISGLPRLQPRFYSISSSPKMYPDQIHITAVVLKYPSGSRMIYGCGTNYILNAKMFLHGEKERMEHQPIDQRSSGAPHYTLSGPRGKYLEDGTKLKIPIHVRRSNFRLPTSPKIPVIMIGPGTGVAPFRAFVQERMMLARKAKAKAKEANGLQGEADALNDWAEIMLFYGCRKEEEDFLYKNEWPEYAEEFNGKFKMFTAISRRPELGGKKEYVQDLIKLQTERIYKMIIEQKGYIFICGDGKSMVKCVEESLKTIFESTGTKNGAAELKFLKDRNRLLLDVWS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGJ5</accession>
    <name>E3KGJ5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ubiquitin thioesterase OTU</fullName>
            <ecNumber evidence="11">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_08606</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Hydrolase that can remove conjugated ubiquitin from proteins and may therefore play an important regulatory role at the level of protein turnover by preventing degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 16 17">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="11"/>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83420.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327839.2">
        <property type="nucleotide sequence ID" value="XM_003327791.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KGJ5"/>
    <dbReference type="FunCoup" id="E3KGJ5">
        <property type="interactions" value="229"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KGJ5"/>
    <dbReference type="MEROPS" id="C85.007"/>
    <dbReference type="GeneID" id="10545137"/>
    <dbReference type="KEGG" id="pgr:PGTG_08606"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08606"/>
    <dbReference type="eggNOG" id="KOG3288">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049327_0_0_1"/>
    <dbReference type="InParanoid" id="E3KGJ5"/>
    <dbReference type="OrthoDB" id="65596at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030968">
        <property type="term" value="P:endoplasmic reticulum unfolded protein response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22745">
        <property type="entry name" value="OTU_OTU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.80:FF:000016">
        <property type="entry name" value="Putative ubiquitin thioesterase otu1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048857">
        <property type="entry name" value="OTU1_Ubl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057766">
        <property type="entry name" value="Znf-C2H2_OTU1-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13312">
        <property type="entry name" value="HIV-INDUCED PROTEIN-7-LIKE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13312:SF0">
        <property type="entry name" value="UBIQUITIN THIOESTERASE OTU1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21403">
        <property type="entry name" value="OTU1_UBXL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24560">
        <property type="entry name" value="zf-C2H2_OTU1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="7 11" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="OTU" evidence="13">
        <location>
            <begin position="161"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="77"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="79"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="120"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EFP83420.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="377" mass="40597" checksum="3E1BD73D3447C43D" modified="2012-04-18" version="2">MASSMLTLRIRGPEGSSTIKISPQATITELYHQISEQTNIKKENLQVRVGFPNPQSISASDTGATVESVNIKSGEVLQASSSQGSSSNGASPAPSTPGQSTPLSKPARVTNDVGRAPAKSSLSSLARVQDQSSASPSASKIENQDGLDSVSASVALEAGHLVLRVVPDDNSCLFRAAGILLDHGDEHSSSSDLSHRLRQIVADAVKNDPVTWCEPILGRDPDLYISKILDKDVWGGAIELAILADHFQTEICSIDVQTGRVDRFGQSHNYANRIFLVYSGIHYDAITLSPIPPEELSNQSTCFPPELDFDTTIFPSDEDSFLHAALQLVSQLRQMHYYTDTASFSLRCEICKVALVGEKDARSHAESTGHTQFGEYA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3KH84</accession>
    <name>E3KH84_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09372</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328078.1">
        <property type="nucleotide sequence ID" value="XM_003328030.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KH84"/>
    <dbReference type="STRING" id="418459.E3KH84"/>
    <dbReference type="GeneID" id="10532345"/>
    <dbReference type="KEGG" id="pgr:PGTG_09372"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09372"/>
    <dbReference type="eggNOG" id="ENOG502SBKV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_866535_0_0_1"/>
    <dbReference type="InParanoid" id="E3KH84"/>
    <dbReference type="OMA" id="KMATINR"/>
    <dbReference type="OrthoDB" id="6222486at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032299">
        <property type="term" value="C:ribonuclease H2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006401">
        <property type="term" value="P:RNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd09271">
        <property type="entry name" value="RNase_H2-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.680">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013924">
        <property type="entry name" value="RNase_H2_suC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47204">
        <property type="entry name" value="OS02G0168900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47204:SF1">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08615">
        <property type="entry name" value="RNase_H2_suC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="136"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="147"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="339" mass="37219" checksum="EE21CF7D11A436CE" modified="2011-01-11" version="1">MQTQPRPSILIRHTHELKHEPEEGGQAITGGDKLDLMPFSIDYHGPAEISKYFLTRPVSTNAPSSSSTTDNKPEEAGEQLECYEGSFRGRCLHGTRLKIPDGYSGLVLSSSDAHPAARSPDQTSISKPSLAPTHSTRNNTTTGTIRGRGRGRGRARGRRLGQAVVTQPPVTRIQRKRKQGKVGAFLDSDEDDDEDEDDDDHMKNKQNQAIIEISSDSHPEPSRSSDLDQQDVKEPRTDRETLVPKTEPPPSSPPLVALEKAVSSPPESKPNPIIHLSSDPATTSAQNTQSKVLTSIGHFEQITIWNPDHPLDLTEDVYARAINEWVGLSHLIHSLPPTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJM2</accession>
    <name>E3KJM2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Expansin-like EG45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10217</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178290">
        <property type="protein sequence ID" value="EFP84497.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328916.2">
        <property type="nucleotide sequence ID" value="XM_003328868.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJM2"/>
    <dbReference type="GeneID" id="10546007"/>
    <dbReference type="KEGG" id="pgr:PGTG_10217"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10217"/>
    <dbReference type="HOGENOM" id="CLU_083686_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJM2"/>
    <dbReference type="OrthoDB" id="5823761at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22278">
        <property type="entry name" value="DPBB_GH45_endoglucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007112">
        <property type="entry name" value="Expansin/allergen_DPBB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22514">
        <property type="entry name" value="EXPB1_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50842">
        <property type="entry name" value="EXPANSIN_EG45"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Expansin-like EG45" evidence="1">
        <location>
            <begin position="41"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84497.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="263" mass="28528" checksum="2CB9BAD36F58026C" modified="2012-04-18" version="2">MTWMIPAAGTASLTHYDLPLNYLASYVTRAQIATIRAKPHRCGCAYGASYHATAALSSLAYSGDERQSIGPGPGCGRCFRLTLRSAHGVDPPFVPADPKPSVVVKIVDKCPSPEFCAATRTHPNKIGQQIHFDLALPSPALNLSFFPSNVELYGYSDFGIWNIDYETVSCEEWAGWTNASSLGVERSYPGYEESCCPTNPPYTNEVCAKSIKAPPPATTYKSPAASSPPPPNPRLIFLCASAWLSLSLTDSFPLFLRLLDTHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLE0</accession>
    <name>E3KLE0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11284</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP85115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329534.1">
        <property type="nucleotide sequence ID" value="XM_003329486.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLE0"/>
    <dbReference type="GeneID" id="10527096"/>
    <dbReference type="KEGG" id="pgr:PGTG_11284"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11284"/>
    <dbReference type="HOGENOM" id="CLU_054616_0_0_1"/>
    <dbReference type="InParanoid" id="E3KLE0"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="377" mass="42585" checksum="D50A287C9A976C65" modified="2011-01-11" version="1">MLDLNELIQGDPDDPATQLEAGDDDHYDSSSEEHDSPPSTHMEITDPNNMFAALPAHDVTMTDQSSSSYPNTNIEQICSDLQSKLKLNPEHLEIALLESKATPEARHASLVFTNGAYHQINYQDSGSQKAGFAYGSTFRDFVRASARMFLLKPTLEAYSNNPHQNGEIPKTLFYLTLDAVEKQPDAWKTDHLPTSGLRANPDVLNHYREVIGDLLKYQRSNLRTLLCYFFLLADILVTKKKPVVGVIPNRGELLASIYEELPPKSSKLNKSEIKHQVRSNFAMRARMCYARLVMVHYYVHRSKKESQWVEIDERLGILRGSSFDFQKKHADVVLEKDAALFSHKKKFCDLSHTDLTVPTLEDVRNAIATAHSPGLNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLL1</accession>
    <name>E3KLL1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP85186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329605.1">
        <property type="nucleotide sequence ID" value="XM_003329557.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLL1"/>
    <dbReference type="GeneID" id="10527169"/>
    <dbReference type="KEGG" id="pgr:PGTG_11355"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11355"/>
    <dbReference type="HOGENOM" id="CLU_1918109_0_0_1"/>
    <dbReference type="InParanoid" id="E3KLL1"/>
    <dbReference type="OrthoDB" id="10277773at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172703" evidence="1">
        <location>
            <begin position="19"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="132" mass="14978" checksum="F2E7A69C6AAC0C40" modified="2011-01-11" version="1">MELLIKSTLALLLTSTLAGPLAVQGDDQTNAFYTCIAPNFPEGYCATKLAPDSQKKVYYDVRRANRYFDKPFHFDCLGSNKENRYCCRRELREINDPPPGKFIVAQDEFEHGRPYDNVPPDYVNCYAAPADS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLT7</accession>
    <name>E3KLT7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phospho-2-dehydro-3-deoxyheptonate aldolase</fullName>
            <ecNumber evidence="8">2.5.1.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_11455</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>D-erythrose 4-phosphate + phosphoenolpyruvate + H2O = 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:14717"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58394"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="2.5.1.54"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the class-I DAHP synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.54" evidence="8"/>
    <dbReference type="EMBL" id="DS178294">
        <property type="protein sequence ID" value="EFP85286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329705.1">
        <property type="nucleotide sequence ID" value="XM_003329657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLT7"/>
    <dbReference type="STRING" id="418459.E3KLT7"/>
    <dbReference type="GeneID" id="10527276"/>
    <dbReference type="KEGG" id="pgr:PGTG_11455"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11455"/>
    <dbReference type="eggNOG" id="ENOG502QPSU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030903_0_1_1"/>
    <dbReference type="InParanoid" id="E3KLT7"/>
    <dbReference type="OMA" id="DINTGLR"/>
    <dbReference type="OrthoDB" id="4699125at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003849">
        <property type="term" value="F:3-deoxy-7-phosphoheptulonate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006218">
        <property type="entry name" value="DAHP1/KDSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006219">
        <property type="entry name" value="DAHP_synth_1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00034">
        <property type="entry name" value="aroFGH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009395">
        <property type="entry name" value="PRK12755.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE DAHP SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21225:SF12">
        <property type="entry name" value="PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE, TYROSINE-INHIBITED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00793">
        <property type="entry name" value="DAHP_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001361">
        <property type="entry name" value="DAHP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAHP synthetase I/KDSA" evidence="9">
        <location>
            <begin position="78"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR001361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP85286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="388" mass="42596" checksum="26B669D8E6BE6FCF" modified="2011-01-11" version="1">MTVTTSGTKSWSISSAFKSSDKRVEGNKTDSSEKLLSPAAMCLEIPLSLKAEKSVYTARDVCAQIIVWEGRPLDRRYPEQALEYARKLKASLSLFPDLVILMRSYFEKPRTTVGWKGLINDPDLNGTFQINKGLWIARKLLRDLTELGLPLAVELLDTFSPQYLSDFISWGAIGARTTESQPHREMASGRPHPVGFKNGTDGSIGVAIDAIKSAAAPHAFVAMNHEGVASISRTPGNPDLHLILRGGNKGPNYAEIDVAEAIKSISKQMPLKNPSIMIDCSHGNSQKDHRNQHKVVHSICDQLKAGNPFINGVMLESNIHEGRQELPSKGPGGLKYGVSITDACIDFENTIQILCDLQRTVVHRRSLLDTLLADDNKTPFGLLPSIFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>E3KMN8</accession>
    <name>E3KMN8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Thioredoxin reductase</fullName>
            <ecNumber evidence="8">1.8.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_11919</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>[thioredoxin]-dithiol + NADP(+) = [thioredoxin]-disulfide + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20345"/>
            <dbReference type="Rhea" id="RHEA-COMP:10698"/>
            <dbReference type="Rhea" id="RHEA-COMP:10700"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:50058"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.8.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="9">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <dbReference type="EC" id="1.8.1.9" evidence="8"/>
    <dbReference type="EMBL" id="DS178295">
        <property type="protein sequence ID" value="EFP85563.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329982.2">
        <property type="nucleotide sequence ID" value="XM_003329934.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KMN8"/>
    <dbReference type="FunCoup" id="E3KMN8">
        <property type="interactions" value="229"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KMN8"/>
    <dbReference type="GeneID" id="10544123"/>
    <dbReference type="KEGG" id="pgr:PGTG_11919"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11919"/>
    <dbReference type="eggNOG" id="KOG0404">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031864_5_1_1"/>
    <dbReference type="InParanoid" id="E3KMN8"/>
    <dbReference type="OrthoDB" id="371245at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000064">
        <property type="entry name" value="Thioredoxin reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050097">
        <property type="entry name" value="Ferredoxin-NADP_redctase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008255">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_2_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005982">
        <property type="entry name" value="Thioredox_Rdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01292">
        <property type="entry name" value="TRX_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48105">
        <property type="entry name" value="THIOREDOXIN REDUCTASE 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00573">
        <property type="entry name" value="PYRIDINE_REDOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 8" id="KW-0274">FAD</keyword>
    <keyword evidence="2 8" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4 9" id="KW-0521">NADP</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7 8" id="KW-0676">Redox-active center</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="11">
        <location>
            <begin position="21"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="336"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP85563.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="356" mass="38192" checksum="664F619B195108D0" modified="2012-04-18" version="2">MAPTTSAAEGSTNPNGKSHNKVVIIGSGPAGHTAAIYLARANLEPVMFEGMLANGFAPGGQLTTTTDVENFPGFPEGIRGPEMMDLFRAQSIRFGTKILTETVSKIDLSNRPFKYWREGHESDESETADSIIIATGASARRLNLPGEDTYWQSGISACAVCDGAVPIFRDKPLAVIGGGDSACEEATYLTKYASKVFVLVRRDQLRASKVMAKRLMNHPKVTILWNTVPVEAKGDGNLLNSLLVKDTKTGEERTIDINGLFYAIGHEPATALVRGQLKTDEDGYICTKPGTSETSIPGVFAAGDVQDKKYRQAITSAGTGCIAALECERFLAEEEAMDRDGEPVVPQPGYLGTDKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>E3KN12</accession>
    <name>E3KN12_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_10977</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EFP85648.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330067.2">
        <property type="nucleotide sequence ID" value="XM_003330019.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KN12"/>
    <dbReference type="GeneID" id="10544214"/>
    <dbReference type="KEGG" id="pgr:PGTG_10977"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10977"/>
    <dbReference type="eggNOG" id="KOG1339">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013253_1_4_1"/>
    <dbReference type="InParanoid" id="E3KN12"/>
    <dbReference type="OrthoDB" id="15189at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05471">
        <property type="entry name" value="pepsin_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034164">
        <property type="entry name" value="Pepsin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF51">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174014" description="Peptidase A1 domain-containing protein" evidence="4">
        <location>
            <begin position="21"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="5">
        <location>
            <begin position="1"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP85648.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="411" mass="45313" checksum="7937D5BB1FF2C750" modified="2012-04-18" version="2">MWKFFSVIAILVSLPLSSITISSSIGADLNNAHPLRIPLEKKLFVRGGDGIFNFTAAQLSHQHTLRKYRGTCEKYKHARSFWPSLSKFSRPYRHHRRQIERLTNQGNQRGREIFGFRQSIAKITHAALRIFIMHLQAAHLQDFRIHLRLIFGSWLWRAGIDGDGSTTSGNQYRDSVKLAGITIANQTFAGVSKLSDEFQHDPTDGVLGLAYPAISHLKSPSFIENAFNQGLIPSKTFSFKLANEGGELYLGGVNPSRFVGTLEAHPVIRQAYWEIGNGSVNVNSINISQNGRMILDSGTTIIYGPKLAVEKLYSSVDGAKKYEKRDGLWKFPCVSAPSVSFSWNGGGQWAIDPKRMSLGKVEANSAECIGAISSQAVGLNDSWLLGDTFMSNVYSVFNPVGGGTVSFATPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNB0</accession>
    <name>E3KNB0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_11075</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EFP85746.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330165.2">
        <property type="nucleotide sequence ID" value="XM_003330117.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNB0"/>
    <dbReference type="GeneID" id="10544312"/>
    <dbReference type="KEGG" id="pgr:PGTG_11075"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11075"/>
    <dbReference type="HOGENOM" id="CLU_1759720_0_0_1"/>
    <dbReference type="InParanoid" id="E3KNB0"/>
    <dbReference type="OrthoDB" id="99432at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP85746.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="148" mass="17057" checksum="B16D9C4F0937191E" modified="2012-04-18" version="2">MIHHPSEVCDISCPTYIQLSKRAQKAIEIKTFTMSAERQKSCFEKINEDEAVDAPTPDASLEFYSNQIQEKQSCNDIKSDADTISYLEWSELNRGFNEHNNHDKMILRIKYDKIQVPTTKIQSGQPTTIDFKDISGKQQMYSDIPYLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNY5</accession>
    <name>E3KNY5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_11966</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178298">
        <property type="protein sequence ID" value="EFP86010.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330429.2">
        <property type="nucleotide sequence ID" value="XM_003330381.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNY5"/>
    <dbReference type="STRING" id="418459.E3KNY5"/>
    <dbReference type="GeneID" id="10540006"/>
    <dbReference type="KEGG" id="pgr:PGTG_11966"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11966"/>
    <dbReference type="HOGENOM" id="CLU_1120600_0_0_1"/>
    <dbReference type="InParanoid" id="E3KNY5"/>
    <dbReference type="OrthoDB" id="2506039at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP86010.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="248" mass="28280" checksum="AC58A8BB1A8EAD6D" modified="2012-04-18" version="2">MERHRHTYQAKASVKTIWKTLVQTKPPAILGPRQECFARCHAQLVRRRFTASLAYPPKNATHDDQLDDWEDTNSDTTEASLNFQDTTLLHIQKAVPNTNFVSLVMCTNIISRKSTSHADLEKFLQAYSLYTESSKLVFDSPKIVPNHHYALHTPDQMKWWGPLSNVSEFSGKKFNGLLQKMKTNAIIGQIEGTVLCEFCQMQRLNAQASSWSLQPDNKLEPKPKRMVEVHPDLYSALLQKLQSKDAAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KP42</accession>
    <name>E3KP42_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_12023</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178298">
        <property type="protein sequence ID" value="EFP86067.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330486.2">
        <property type="nucleotide sequence ID" value="XM_003330438.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KP42"/>
    <dbReference type="GeneID" id="10540072"/>
    <dbReference type="KEGG" id="pgr:PGTG_12023"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12023"/>
    <dbReference type="HOGENOM" id="CLU_2251402_0_0_1"/>
    <dbReference type="InParanoid" id="E3KP42"/>
    <dbReference type="OrthoDB" id="10548252at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP86067.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="104" mass="11521" checksum="9ACE3BB953B3C189" modified="2012-04-18" version="2">MHGLCDIWCIKPPWAQSQVLCGQVPQGLDWATCVGLGSWHILKSGLANPKLLANQIIDTPRSQCSPPHLVQHTNKNMSDSAVMNLNKLEIEADKKRLQALVQEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQ16</accession>
    <name>E3KQ16_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_12347</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178300">
        <property type="protein sequence ID" value="EFP86391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330810.1">
        <property type="nucleotide sequence ID" value="XM_003330762.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQ16"/>
    <dbReference type="GeneID" id="10540405"/>
    <dbReference type="KEGG" id="pgr:PGTG_12347"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12347"/>
    <dbReference type="eggNOG" id="ENOG502S5VT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078842_0_0_1"/>
    <dbReference type="InParanoid" id="E3KQ16"/>
    <dbReference type="OMA" id="IDAHPFF"/>
    <dbReference type="OrthoDB" id="2520628at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP86391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="282" mass="29865" checksum="D2CAF7D7C33C7DA6" modified="2011-01-11" version="1">MVHSAAPAFIQPVSALFDALATPIHPTFFPVGCFPLLHAFRISVAYRTLNTRAGGKTSAAADLAGFLLMAWGGSIISHILLNLPIPQLLSFSPFLVYATTHMSLPDLGSLPSLKTLDSLFPLVDGLLRTASIAAGVEACRNHGSPAIRESLSIQLFIGAVASSGGGISAQTLSVWEPSWRLNRPLFLQEGTLLAGTDVWSGALVAAIYGCLSASHPHYSSLIGLLYSSSFTNSPILSPTDAKATAASILTLIYCWRVYHVHYSKSSVVGTKKAGQKSKSKNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQN7</accession>
    <name>E3KQN7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12994</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178301">
        <property type="protein sequence ID" value="EFP86612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331031.1">
        <property type="nucleotide sequence ID" value="XM_003330983.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQN7"/>
    <dbReference type="GeneID" id="10540873"/>
    <dbReference type="KEGG" id="pgr:PGTG_12994"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12994"/>
    <dbReference type="HOGENOM" id="CLU_045572_0_0_1"/>
    <dbReference type="InParanoid" id="E3KQN7"/>
    <dbReference type="OMA" id="HEPMASK"/>
    <dbReference type="OrthoDB" id="2498923at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="419" mass="46913" checksum="96E9B28DB8B3C378" modified="2011-01-11" version="1">MVFLPPGTNQTPLSDPTGPNGSGTNHGSSTPGYRRQRSPSDPDDDPSPRQRARTRERASELVNPFLDIDGPDLDEQLPDPDTLDATRPPAENLTVALGAQVLTSGQLAMLAEVSHILSPLSPPPHFDLNQPLPTTIFRPQIPQPEHTTIILSTLAAVLKRLDTLTQQQSARTPLDPAPAANLSLAEQVRMFQFSPEAKEFLRQQARESMMHPDLEAYCEDTRDRGLFRMVLSGIIRQPATYLRAQFPPGFVAQDRLSMNHTHTEAKRQLKNVRHQLRNLLLTGVLTSNAEAPPIPNLTKLARDVWRFLMGTATRLSNEEVDTRVLPLLKIRIAYLRLATLENHFDPLARNVSQWDQIDSQLQANRERTVNFTNSWHKMIYLKDEQLFSTSPALADLDTSLCKCPTDREVLDRMASLGEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQX4</accession>
    <name>E3KQX4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="21">Dicer-like protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PGTG_13081</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Dicer-like endonuclease involved in cleaving double-stranded RNA in the RNA interference (RNAi) pathway. Produces 21 to 25 bp dsRNAs (siRNAs) which target the selective destruction of homologous RNAs leading to sequence-specific suppression of gene expression, called post-transcriptional gene silencing (PTGS). Part of a broad host defense response against viral infection and transposons.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="12 13">Belongs to the helicase family. Dicer subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178301">
        <property type="protein sequence ID" value="EFP86699.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331118.2">
        <property type="nucleotide sequence ID" value="XM_003331070.2"/>
    </dbReference>
    <dbReference type="FunCoup" id="E3KQX4">
        <property type="interactions" value="309"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KQX4"/>
    <dbReference type="GeneID" id="10541122"/>
    <dbReference type="KEGG" id="pgr:PGTG_13081"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13081"/>
    <dbReference type="eggNOG" id="KOG0701">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000907_4_6_1"/>
    <dbReference type="InParanoid" id="E3KQX4"/>
    <dbReference type="OrthoDB" id="416741at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004525">
        <property type="term" value="F:ribonuclease III activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050688">
        <property type="term" value="P:regulation of defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030422">
        <property type="term" value="P:siRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18034">
        <property type="entry name" value="DEXHc_dicer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00593">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.380:FF:000004">
        <property type="entry name" value="Dicer-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001669">
        <property type="entry name" value="Dicer-like protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000628">
        <property type="entry name" value="Endoribonuclease Dicer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1520.10:FF:000033">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1520.10:FF:000040">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.380">
        <property type="entry name" value="Dicer dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1520.10">
        <property type="entry name" value="Ribonuclease III domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038248">
        <property type="entry name" value="Dicer_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005034">
        <property type="entry name" value="Dicer_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000999">
        <property type="entry name" value="RNase_III_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036389">
        <property type="entry name" value="RNase_III_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950">
        <property type="entry name" value="DICER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14950:SF37">
        <property type="entry name" value="ENDORIBONUCLEASE DICER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03368">
        <property type="entry name" value="Dicer_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00636">
        <property type="entry name" value="Ribonuclease_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00535">
        <property type="entry name" value="RIBOc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69065">
        <property type="entry name" value="RNase III domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51327">
        <property type="entry name" value="DICER_DSRBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50142">
        <property type="entry name" value="RNASE_3_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="2" id="KW-0930">Antiviral protein</keyword>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0347">Helicase</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="13" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="16">
        <location>
            <begin position="28"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="17">
        <location>
            <begin position="346"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="domain" description="Dicer dsRNA-binding fold" evidence="18">
        <location>
            <begin position="540"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="domain" description="RNase III" evidence="15">
        <location>
            <begin position="946"/>
            <end position="1067"/>
        </location>
    </feature>
    <feature type="domain" description="RNase III" evidence="15">
        <location>
            <begin position="1113"/>
            <end position="1265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1393"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1393"/>
            <end position="1404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="1405"/>
            <end position="1419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00035116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EFP86699.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="21">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1431" mass="161492" checksum="C37CD008DAF71CDD" modified="2012-04-18" version="2">MDTKNPGPTGGLHRPDLIKIPRLYQTELLEEAKKRNIIIRADTGTGKTIVAVNLIAWTAVKTKANNKNHQIQAFLVPTRPLAHQQAEYIQKHCTLRIKAYTGDLQPELWNIRKWHSELNEVDVLVSTAQIFYDLISKGYWKLEDVSLLIFDEAHHCRKNHVYNQIMRSHYHRLAKDSTVRLPKILGLTASPIWNFKDLEKAESDIKNLQSALAAQIYEVKKHTEDVCRHNFKPNEQVVYFEPAPDFQQLSHPPWDQINKLLSLHASPKMIAAIQSVSLDLGTYAYSLAVLDWLKSLLTIGGSNQLMSGRLVDPNYQERIQSLIKEVEKLVDIDDIPENQLSSKVIALYKILVNYKEKDDHNNFLCIVFVERRQHAQLLPILLERNVQLKDFLRPAALTGHGSGNENDLIGIKMDSKTQNRTVAKFRTGEHNLTIATSVAEEGLDFRSCRVVIRFDLITTWKGYIQSRGRARARESDYIVMLPTGTANKYLAFSGKEEQLKAALYDRPQDELIEDGEEESTPQLICQLADGKESILTYSAATSLLNDVCQLIPPDEFLPVVAPEYEMMELGERFKCRVTLPPMAALPPSRRTFTGLEMGTKNGAKRSAAFEACKVLRELGVLNEHFLPQREDKVAQVRDADGREIETTQLADKVEAIISNVYGDFRTSSEVWLHKLSFPDDTPAGFSTMGLLCARHLTIPDDLQLFDHDPVSRPLSVKIKHSKKVEWEKDAAPFNLQRLERFTLLVMQVAVNRKPYEGKLYFLVAPLLNDAIEIDWNLVDTPMIPLSEAADSSRYQCTIVPSRRPRFRIFDTCESAGDLCAASPPSRVPACPSMQHFCKKIAKFHNLGHFYKTCYDLEDEHLKGGFVYLQTTFQVYNNLLQSESTLAHPYRTLLPLKLCQGTHIPRTIWKVFSYLPSLTRLLHDSIQATALFMRLDFPMTLLPRGIQALTPPGVGVPWDYQTLETVGDAFLKLATSVHVYLSHLRKGEGDMSHVRSKSVDNDYLRRKAIQANLPSSILGQRFRTDRFRDAQTEDGKELPNGNFSRNVPRRVLSDVVEALLGAGFLTGGIELGLKIGTALDLCFGGTTPWNERTLNIAFEGTAHDKLASPILLKCQALEEKIGYVFKEKLLLVQALTHRSANSFMTNCYEREEWLGDAVVDMWIVQHAYKRFDNATAEELTLARAKLVSNGSLGFLAIKKLGLHKMIMHKSESFEQACVEAIKAIEPFTSIEEFFSNLENLFVVFDPPKILNDVLEAIVGAVFIDSRFDLPTVYRTIDKIFEDVTPGLSRIVARDPLSTMLRLRDQYQCAELRRVSTTIKEPNPEGEDKDPISVKVCRIELHGQEIASGRHASSASVAEQRASLAALKVLQDPSGDSRAPKSAWSRCQCKARAVSTTAVASSTPAAESKEEIQKEEGRHASQDLPRQMSHLHM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3KR22</accession>
    <name>E3KR22_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glycolipid transfer protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_13129</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178302">
        <property type="protein sequence ID" value="EFP86747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331166.1">
        <property type="nucleotide sequence ID" value="XM_003331118.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KR22"/>
    <dbReference type="STRING" id="418459.E3KR22"/>
    <dbReference type="GeneID" id="10541171"/>
    <dbReference type="KEGG" id="pgr:PGTG_13129"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13129"/>
    <dbReference type="eggNOG" id="KOG3221">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079400_0_0_1"/>
    <dbReference type="InParanoid" id="E3KR22"/>
    <dbReference type="OMA" id="EMHGAEW"/>
    <dbReference type="OrthoDB" id="205255at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902387">
        <property type="term" value="F:ceramide 1-phosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902388">
        <property type="term" value="F:ceramide 1-phosphate transfer activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035627">
        <property type="term" value="P:ceramide transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120009">
        <property type="term" value="P:intermembrane lipid transfer"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3520.10:FF:000001">
        <property type="entry name" value="Pleckstrin domain-containing family A member 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3520.10">
        <property type="entry name" value="Glycolipid transfer protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036497">
        <property type="entry name" value="GLTP_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014830">
        <property type="entry name" value="Glycolipid_transfer_prot_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10219">
        <property type="entry name" value="GLYCOLIPID TRANSFER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10219:SF25">
        <property type="entry name" value="PLECKSTRIN HOMOLOGY DOMAIN-CONTAINING FAMILY A MEMBER 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08718">
        <property type="entry name" value="GLTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110004">
        <property type="entry name" value="Glycolipid transfer protein, GLTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Glycolipid transfer protein" evidence="2">
        <location>
            <begin position="26"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP86747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="200" mass="22813" checksum="1EE6439D90CFB90B" modified="2011-01-11" version="1">MAADNTCYFDQMKRSFADVPVTDEGVDTLAFLEACEDLVRLFDLFGSKAFTVVQNDLSGNIAKIRTRYDACPEKSKTLELLVENEKAEKKKDATQGLLWLTRGLHFTYEGLRLSQKNPGDELSVSFTKGYEVTLKQYHSFVVKPLFGLAMKACPYRADLYEKMGTKERVDTELERWLSALEMIVNRIQSFYEKGNYGKGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3KSG3</accession>
    <name>E3KSG3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_12833</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178305">
        <property type="protein sequence ID" value="EFP87249.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331668.2">
        <property type="nucleotide sequence ID" value="XM_003331620.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KSG3"/>
    <dbReference type="GeneID" id="10537648"/>
    <dbReference type="KEGG" id="pgr:PGTG_12833"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12833"/>
    <dbReference type="eggNOG" id="ENOG502SJ9D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_295459_0_0_1"/>
    <dbReference type="InParanoid" id="E3KSG3"/>
    <dbReference type="OrthoDB" id="3362851at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF89">
        <property type="entry name" value="ZN(2)-C6 FUNGAL-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="720"/>
            <end position="945"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="957"/>
            <end position="1008"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="757"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="782"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="826"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="837"/>
            <end position="847"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="884"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="962"/>
            <end position="1000"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP87249.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1008" mass="114142" checksum="082B5C7F7F66F265" modified="2012-04-18" version="2">MPKELAKPKQTRKRIPRTCAQVSYQAKDPYYIIVELFTDILHTTLISIAPVKRKQKCDSLKPCAVCKLRGEADLCDYSGAQPTRVLDEEDEISNQVSSEELKVLRQQVDDLEHAVTLVVNRPLGEASPTLGHNDVTHRLAIDMDELNLTNILSSFLVCRMVSSEFIQEFPIIQKVRHQCRLDQNLPPVPNTTIYASSGLSTYFSNQLNKSHYTELRNLLPTQQQSSVFLATYMKEFGWFHSCFNAETYTAALDNLYTSSPTGSEFDKDPQARFITIATCFAIARSAIIKLSSQSLIELNLPCEPSARLEQSRMWLNMSVACLKCADFEVNPLLESINCLIILLEVVWFDEIVGGIEDLSTLFDLKCKAVHLAFDLSLHRDPSHRDPTGMVHVNPAIARERRMAWWALLSIDGLYSGLTGRMSSIIGLEAVDVFLPALSTTSYPEEPHDGCEQGCTTSTPATAIKPRLLLGYVGHEIARLPHQRTPLPTINDIHQAHRDLVSLEVHLSDSYKLHIINGHCIDRTRLPPCSNARRDAIYFYMRYHYLFVKLHSPLHFIRKGEDIPGIETKHPYHRSAVVDHALLLLDLRVLGNFSPDYIYGNSMVIEASFSLALDYLYDPKSEVANVIKVELQNYCKFLQSSKVWLIKRGLNILQCLMATWGSPPPKGTVSWFSVNKPTLSPCSWIQGPYHQPLEITNFSGTGETQSRLEKLTQAEPSMQVFDSPDHVANGQQALAGPPWPLPGTLIGPHSPSGPLPAQSVSSTHYQQSQQHQPHPQLPEYNHSGISSENSNEFSQFTHQPGPPRSQTIVHPDPFHQFSILPTASTRGHPDGQYEHKNNNNQDPNNRPNTSAENGMNLDDFLSGVNGPPHPGSASGGPPRFENGHHHQLPATHHHHHHHHQSELASNLQPNGPPTQHHHLHGHHHQLDPHHLHQHHHYQQQQQLQHHQIDQFSHPALPTHHLHQQQQQQQQQQQQQQQQQQQQQHQQQQQQQQQQQQQQQHQYSPTHAKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3KT59</accession>
    <name>E3KT59_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_13673</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178306">
        <property type="protein sequence ID" value="EFP87445.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331864.2">
        <property type="nucleotide sequence ID" value="XM_003331816.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KT59"/>
    <dbReference type="STRING" id="418459.E3KT59"/>
    <dbReference type="GeneID" id="10538175"/>
    <dbReference type="KEGG" id="pgr:PGTG_13673"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13673"/>
    <dbReference type="HOGENOM" id="CLU_2414367_0_0_1"/>
    <dbReference type="InParanoid" id="E3KT59"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="2">
        <location>
            <begin position="6"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP87445.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="92" mass="9961" checksum="75FB8D50ACA70CCB" modified="2012-04-18" version="2">MMTVYPQAWNLLQHVEKFKSLAGQAKNGAPPLTQSQTPQVPGTPSPPNATDAASNASSRSKDWERPTGAHAAKQHVNKEEYKRKNQAHGGNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3KTN2</accession>
    <name>E3KTN2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_13415</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178308">
        <property type="protein sequence ID" value="EFP87629.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332048.2">
        <property type="nucleotide sequence ID" value="XM_003332000.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KTN2"/>
    <dbReference type="GeneID" id="10538860"/>
    <dbReference type="KEGG" id="pgr:PGTG_13415"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13415"/>
    <dbReference type="HOGENOM" id="CLU_2237930_0_0_1"/>
    <dbReference type="InParanoid" id="E3KTN2"/>
    <dbReference type="OrthoDB" id="10609188at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP87629.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="105" mass="11908" checksum="64DC405923F2BFAA" modified="2012-04-18" version="2">MDMFHSHQQRRATNSLQDRVLLVRPIPTASRFTWVTHFWKEVSEHLVTSQHSTFWCPGTAGSTASQDKRIVRRQVDVVIIKSHSTIQLSTTDSVALSPTGLCNFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KUI9</accession>
    <name>E3KUI9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6589 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_13743</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178310">
        <property type="protein sequence ID" value="EFP87939.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332358.2">
        <property type="nucleotide sequence ID" value="XM_003332310.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KUI9"/>
    <dbReference type="STRING" id="418459.E3KUI9"/>
    <dbReference type="GeneID" id="10534918"/>
    <dbReference type="KEGG" id="pgr:PGTG_13743"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13743"/>
    <dbReference type="eggNOG" id="ENOG502SCRC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009176_0_0_1"/>
    <dbReference type="InParanoid" id="E3KUI9"/>
    <dbReference type="OrthoDB" id="3207600at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046496">
        <property type="entry name" value="DUF6589"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20231">
        <property type="entry name" value="DUF6589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6589" evidence="2">
        <location>
            <begin position="251"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="720"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="114"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP87939.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="756" mass="85068" checksum="70B52B5A50E23EC6" modified="2012-04-18" version="2">MAFKQRFWATNQGWDSTKNLLLAIRRLAYRHLDTRQLWEGFILEQATELALSQKPTSGLAPEGSYHTSSTLSNAFFTKEGREAQNKSLTDWMLFLYQLLCSKIEGGKAVGGSDAPEDPVEENSDDKGEDNPLDDLGDYDGSVLKKSKDRASQRANRVHTMARTICAMVAFGGNCCHNGFQLSNALLFLAGGVTEREKVHMKSIGHSSQMFHGTWGYVHLIPPALKSRLDPSQLTIEALNTSLHLGQELEIRPEMFTPTHESTTHFEKTIKSQITRVILKYLATPMDRRVKLHKHPPEVNPITPEDPNITMLKLMVASDNSALGVGEVFTGVIQQSGLTPAKLHSRLQIIEGDLGSCNIFDSLRKQRNPGLGDHNTLDNILPIPGAAHTLWNLSQEIYLGHWGNEKLARDTGAWRTLHALGIPAEKPVTKKDFNLMLSHIEKIHEATLLYCVLVVANRAHEVLSKGLLKVTSETIDDWVERTYERFCSGDALQSELAANYCSHKNLLLRIQDFSTIVKANCAMKDGDYGRLMFMWQRWAVMSQGIGGMPHYSKHLPKLIILLKYILPKSLSDLVLNTLLMSPAGKAGHFVATDFYLEVQNYWLKYFFNHSGIGTDIERLKEIFSSDINLLQYMLQLIKLESGAEVVHQSHKNKLNLDSFNNFRCMALRERLGLIPLGGREPEEINDFYSMGMGKLQREYPQMGLSRFWPYSKGIMSMFEEDERNRGRTNGGDIATELEHQVVSDESSSSDVTDSDSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KW58</accession>
    <name>E3KW58_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ecp2 effector protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14111</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178314">
        <property type="protein sequence ID" value="EFP88533.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332952.2">
        <property type="nucleotide sequence ID" value="XM_003332904.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KW58"/>
    <dbReference type="GeneID" id="10540999"/>
    <dbReference type="KEGG" id="pgr:PGTG_14111"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14111"/>
    <dbReference type="HOGENOM" id="CLU_2050818_0_0_1"/>
    <dbReference type="InParanoid" id="E3KW58"/>
    <dbReference type="OrthoDB" id="2508422at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172882" description="Ecp2 effector protein domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88533.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="120" mass="12637" checksum="1659351918BCA4FA" modified="2012-04-18" version="2">MSVYKFLMISVGLLSYSPLSAAKSCYHALVDVNQCTDAASKIPYSGDDKSMMFTSDKPENVQSGRCSVTVVRETLTDTSAVAPKADIDAAIQTILGCAQHSGMTEVSTKGSSILVTIHAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KWJ3</accession>
    <name>E3KWJ3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14752</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178316">
        <property type="protein sequence ID" value="EFP88786.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333205.2">
        <property type="nucleotide sequence ID" value="XM_003333157.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KWJ3"/>
    <dbReference type="GeneID" id="10541738"/>
    <dbReference type="KEGG" id="pgr:PGTG_14752"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14752"/>
    <dbReference type="HOGENOM" id="CLU_1230418_0_0_1"/>
    <dbReference type="InParanoid" id="E3KWJ3"/>
    <dbReference type="OrthoDB" id="2509374at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="59"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88786.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="225" mass="24294" checksum="B86ED6DBF6E78D1C" modified="2012-04-18" version="2">MFLPKTAGAADALTGGVQGLRAGETLDSGVGAGKGLSNVVDSTNALRGTGTETLKPQDLMTMRGGNEAKSLPKTEDASNLDKTLTKAEDSSKLDQAKADEVEDLDEPRILGRQGKIEEIEKKIKWYAEHMGTSAPPELVKQLEGLKNGEPIKAIKATPAWKPGLQTIHSQKLLEEISETAHSLPAAQKLSMWKKLLTHLLEYFRNSEGILPALVMKLHLKTPPVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KXB4</accession>
    <name>E3KXB4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15190</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178318">
        <property type="protein sequence ID" value="EFP88987.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333406.2">
        <property type="nucleotide sequence ID" value="XM_003333358.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KXB4"/>
    <dbReference type="GeneID" id="10542429"/>
    <dbReference type="KEGG" id="pgr:PGTG_15190"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15190"/>
    <dbReference type="HOGENOM" id="CLU_621317_0_0_1"/>
    <dbReference type="InParanoid" id="E3KXB4"/>
    <dbReference type="OrthoDB" id="2501845at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172914" evidence="1">
        <location>
            <begin position="26"/>
            <end position="518"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88987.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="518" mass="58778" checksum="67E7B8B4681F7888" modified="2012-04-18" version="2">MKHMSLFLVLNLSICLLLFSPIGLALLIPHDRYSVDLRSDSVLGATQTERKSIASYSYLGVEPSKLIQHVIIRSGPYHICTPLSLPLAGANRAIGVQYHWSSIKGCRIHNRLSKDPMKYSRSTSAILTYFYSLSVLSYVQGRIGGLPKLHLTTYSLPEPPQQSKSIPSGAGYHLEYPIPKAVARSKDTASKSTHTSANSVHKNQEGRTLCCFGSVNPRVNSQEKGSASVPKRESREKLEKTLIIEHPAVDPSSHQLQADQMEKLKQWKSIAEVAQFMEKLELGITEIVNSLIYLEMQRFSSEDLERLLARFTENREFVRTPATFAFLVHTFIIDVYRSRVGSNQPNLVANAYLKNEYGIYKRYAREQIFMLAKIEIMDCLDGMISRASRYYEKAKLEIDTWKEQVDSVIEMLKLIQWEMSPFHSQEIQRTLLRNQLSQTKARSGDRGLFEFLETGARMNVSVESLQSFLLLNTKGTTPEHREKLFLHVQSIHDDLSEICDDLDSPKINHRDALRGAVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KXJ6</accession>
    <name>E3KXJ6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15157</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178318">
        <property type="protein sequence ID" value="EFP88954.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333373.2">
        <property type="nucleotide sequence ID" value="XM_003333325.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KXJ6"/>
    <dbReference type="GeneID" id="10542234"/>
    <dbReference type="KEGG" id="pgr:PGTG_15157"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15157"/>
    <dbReference type="HOGENOM" id="CLU_1993742_0_0_1"/>
    <dbReference type="InParanoid" id="E3KXJ6"/>
    <dbReference type="OrthoDB" id="10279456at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173821" description="Secreted protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88954.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="125" mass="13336" checksum="13B3BB2DC8776A67" modified="2012-04-18" version="2">MNMFSGSFVILSLLSLSHADGGREVISSGPGIAYPTSYTRAGPNDPFTVNGSQQCQHCGIYVAKNCHNFNTPNDPPTDQSCDLAFLPHMNHPQESTCKNNKGSFNCYSGRDSRAGKGGCTDCVIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>E3KXV3</accession>
    <name>E3KXV3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_14880</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178319">
        <property type="protein sequence ID" value="EFP89039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333458.1">
        <property type="nucleotide sequence ID" value="XM_003333410.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KXV3"/>
    <dbReference type="GeneID" id="10546504"/>
    <dbReference type="KEGG" id="pgr:PGTG_14880"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14880"/>
    <dbReference type="HOGENOM" id="CLU_2074307_0_0_1"/>
    <dbReference type="InParanoid" id="E3KXV3"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP89039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="118" mass="13214" checksum="C6D6E041C30774E0" modified="2011-01-11" version="1">MALKCTKLQSDGAAGQRSEQFGRLLIQIVVRLGWAEPDDKQGPNHKQSATELSSDRVDLFLFWRVSLKELIAILNTRLPGDVMEAKESSKSGERKKVLSTRFGSFGEEEENGEMCSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>E3KXX0</accession>
    <name>E3KXX0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_14897</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
            <location evidence="1">Centromere</location>
            <location evidence="1">Kinetochore</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mis12 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178319">
        <property type="protein sequence ID" value="EFP89056.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333475.2">
        <property type="nucleotide sequence ID" value="XM_003333427.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KXX0"/>
    <dbReference type="STRING" id="418459.E3KXX0"/>
    <dbReference type="GeneID" id="10546524"/>
    <dbReference type="KEGG" id="pgr:PGTG_14897"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14897"/>
    <dbReference type="eggNOG" id="ENOG502RXKF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_871944_0_0_1"/>
    <dbReference type="InParanoid" id="E3KXX0"/>
    <dbReference type="OrthoDB" id="1884855at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000444">
        <property type="term" value="C:MIS12/MIND type complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051382">
        <property type="term" value="P:kinetochore assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008685">
        <property type="entry name" value="Centromere_Mis12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14527">
        <property type="entry name" value="PROTEIN MIS12 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14527:SF2">
        <property type="entry name" value="PROTEIN MIS12 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05859">
        <property type="entry name" value="Mis12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="9" id="KW-0137">Centromere</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="5" id="KW-0498">Mitosis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP89056.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="375" mass="42259" checksum="87107F87893146C8" modified="2012-04-18" version="2">MATTNEHTTTTFSQDDRLEKLPPLNEQYAIDDLLTEHFGFAPKVFTGHIFNIINEAIYDTVSEVEKAVIESYFPGSAENGTEGLPSSTSTGYPSKDDIAKSLNQLETLLCSSIDNQFDLLEIWLHRNVFNFPNLREELMKHLRFKHMNLWDQFYQLAQPTTSSDAQASADQIVSPDWEKLKLEEEERWAKLEKFKSDYNQSKDDYHSLLAVSNVLDRKLAGLKAVSSQLTQISSEARTIYDPETNKPVDLIGQSTMILGDYQRLLKLDKVRETRERATNFAKSAPQHASHPMTAEEMIEDEQDPSCCAASATPFQTHARAINLLSKAKVDDFLAELADKGALDVDRDEGLVEVHNQLLGKGKTPAANTPLHQDRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZA1</accession>
    <name>E3KZA1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15589</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP89626.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003333997.2"/>
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    <dbReference type="KEGG" id="pgr:PGTG_15589"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15589"/>
    <dbReference type="HOGENOM" id="CLU_536525_0_0_1"/>
    <dbReference type="InParanoid" id="E3KZA1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="79"/>
            <end position="128"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="460"/>
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            <begin position="235"/>
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            <begin position="259"/>
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        <location>
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            <begin position="372"/>
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        <location>
            <begin position="410"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89626.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="575" mass="64703" checksum="706141929395567C" modified="2012-04-18" version="2">MSIENESESAACFLSLSQKLSDIFKLTSDRLNQISDSLSSIIDSQPSLLLQLNPVEFPNQSSRHAPLAAIRTEINHPSQLAAQPAPFPSATSEPLPGPRSDNDPPSLQKRDKHGPVSNSHSGFIPDLRSADLNGQKKLLPCTPPTLCEALPVVSDHVESEGLAEIRNPSSCTPPPPSITPRRLNPEGLVIQDDPTEDFDQAPSVHPNQTENGPVLLNLRERIASPPSPVAVGPALSNSSNLAQRIDPRSSQSAHSSPMDMRDSDPPKEVTPNDRTRPVSPATSSYRRLGDSSYDYSSRTGSSSRRRSRTPSSRSSNQRYRPDSRPSSLRRDSIPRQTSRRPNVYDSYKPGGPTRARRPRSRSLSRRSRSPSRRSPSPSRRSPSPSRRSSLPSRRSSLPRRRSPSPSRRSPSPRRRSPSPRRRSHSPRRRSPSPRRRSPSPSRLPSERLGSKRPSNARFIDRIESREDRDFAEYLDSSRGRPSYDRLKSLLRTLSGPNHSELVVSRHLWETFHRFRHRTDTREEQMLRHLHLYPELMHPFLTCARERHIVTYRPIENSLSKSTVKLDVSCRSPVPR</sequence>
</entry>
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    <name>E3KZE6_PUCGT</name>
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        <lineage>
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            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178324">
        <property type="protein sequence ID" value="EFP89671.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003334042.2"/>
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    <dbReference type="AlphaFoldDB" id="E3KZE6"/>
    <dbReference type="STRING" id="418459.E3KZE6"/>
    <dbReference type="GeneID" id="10545237"/>
    <dbReference type="KEGG" id="pgr:PGTG_15634"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15634"/>
    <dbReference type="HOGENOM" id="CLU_004591_1_1_1"/>
    <dbReference type="InParanoid" id="E3KZE6"/>
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    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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    </feature>
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            <begin position="928"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89671.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="967" mass="110137" checksum="212590D5B4DDFBAC" modified="2012-04-18" version="2">MSNPIVHKHMDFYPEDPKGYNIYKLSQSYKWRANFPEDIRVQMVVVRSKHFYVFEPIELASGKIFIPVYFYTYKGELYSKCVMPKTRTTRDDRGLTHNYLTIPHHMPFESADLVAIPCEQFAKIYSEIIMPGGIPCARWCENAIWGAKTGKTTKFPLPNPWHVRANGRIIRHVPITLYADDTSGNRSKQWNKHISYYYTLSGLPPNMTNQNYNCHFLATSNTAGALELADQIVDELNLLATEGFTAFDAGLNQEVLVMTMALCFLGDSPMHAEIANTPMPSISLNPCRMCTLSAPGKDCKSTHEYVNDFLGKDEHGDKVPFVPRRWEETIQNTHALWEIGMTRSKKLFDSQSIEYGVRDVVNRACLMIIKDRKASKTKKAVIRKMHKEKSLKLFNPFLRLKGFDGCKDTPVEILHVFLLGVVKYLLRDFMTTTIKPKHLPQLIASWDSFIVQPLELGQIQGKYFVKHYKSLVGKHFRIVLQVAPFVLFQFMNEDQRELWSAMCELSPLIFQTTILDMELYLKTLQEKLDNFMYLLIMMSAQWVNKPKFHMLLHLPDSIRRFGPASLVATEKFESFNGLLRNASVHSNRHSPGRDLAISFANYECMRALGSGGDLFNYIENRYFRPSPQVTEMFKDRMIQKAMGYNEQLANKSTTIIASYPTKKAPPISIPQYLQDTYPNKTFEVVITLHLDSHTVLKRNTFVQVKSSESSTTKRIARINSMWHVRSPTSSGYVISVTWFKLLGTSEFYKMRIIASTHVMADIFTKDIQATLNVQHNCHEAQCQFKKRERNIANYQEGDPGLSYETIHNNHNSFILNSTSLHAPVNHLYLADLEHYEPTPSQWVGAIQVGLDKWNVGPNKGKGVAAIQDMNPDHAMGGVEQHNTPEPQGSTQSHTWGSNSPGSHRGIGNTNQTTTTTPNLTNVNPNLQIGTNSQSNYASESFTPNYQSRGDLYQQQTPGAILHTSSSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZJ5</accession>
    <name>E3KZJ5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    <organism evidence="2 3">
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        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178325">
        <property type="protein sequence ID" value="EFP89720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334139.1">
        <property type="nucleotide sequence ID" value="XM_003334091.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZJ5"/>
    <dbReference type="GeneID" id="10545289"/>
    <dbReference type="KEGG" id="pgr:PGTG_15376"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15376"/>
    <dbReference type="HOGENOM" id="CLU_050896_0_0_1"/>
    <dbReference type="InParanoid" id="E3KZJ5"/>
    <dbReference type="OrthoDB" id="2511178at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="357"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="264"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="319"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="358"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="373"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="386" mass="43558" checksum="E3BB841D801895E4" modified="2011-01-11" version="1">MTRDTSSAPQQPSVFASLGSSLPSIAETAMRRPAAEVSQSERGISPALINSRATSLALETMSNRSTPVPWPIDRLPFPVNMNTEMDRQVSTPIPRRNHPTQFPVLEQPLVPQEEDTVMDNSELETVINLFNEQWNMFVQARENQNPRLMRVALIQAISSQEEIRVLAGGAEMLRICENWIAREELADLERAQLAHQNQQATRLAITQTAHEHTISPSPAPQHRSTRQSSDVTFLGAGPMQQGSNPTPPPPPPSTRPPSAIVRQNPAQQMQPYHQQTHPQHREGVYYQQQPQQETIVQNYAPQTRVVPNYAPQPPHHPPQQRSWRGSGRNWRRPQDQTSRLLKVGDYLMRAERIAGRVFRTRGRGRARGRGRRPPPQQEGPPAPNHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3L0C4</accession>
    <name>E3L0C4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16191</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178327">
        <property type="protein sequence ID" value="EFP89903.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334322.2">
        <property type="nucleotide sequence ID" value="XM_003334274.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L0C4"/>
    <dbReference type="GeneID" id="10545718"/>
    <dbReference type="KEGG" id="pgr:PGTG_16191"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16191"/>
    <dbReference type="HOGENOM" id="CLU_463911_0_0_1"/>
    <dbReference type="InParanoid" id="E3L0C4"/>
    <dbReference type="OrthoDB" id="2506555at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89903.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="588" mass="64864" checksum="33CB16690110DDC6" modified="2012-04-18" version="2">MTPEPDDPTIKNNPDFPAHSSPLTELSEATEYNNDDDFQENDASALPEPVWPEVSNESTSAQKPDPPPQAVTPGGYLNDPEVSIISAKEFLDNIKAKAQRLEVICKDTSIPLSARRLMRDAMKIMIDSSTTVQQGKSLPEDEEVRIGNKNLLGKRNKSSTTRKPSKKMRHRFTSGDSEKEGATEKDKTSNSEKDNPNNEVPDDPPSVPDQPNPNPVMETSEGNHQVTQSSVPVDSPSSLAPNPTQAVETSEENRQNPPSSTDGCDETDPIQQKPKSPSRTTGCNESTSSKKNNDHIHQNAPPLSRNEPENSNPDTNPTSILNSNQSVSRDSTRAEEVGIASTDESTPINLKYRTELSPIHELYAATISYNAYHTAFESINQLYSSRTNGLLPPTAYSSFLSSRIEFKPVNGTSLKIWLKNLRREGPDLISPGFNEPFYKTDVWCFPSKTSISSPPSQSEMGSELFTLFTHLCHPAEAVQCKWAAILFQSIALVAKDISSLPVYNKPTDPANELGPELAIIEYFIQCLSGPDASHEITDSTSSKEKENKISFSTLDKFIDNIHQMLIAFAVLKAKYFRKNISQDPIINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3L184</accession>
    <name>E3L184_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16305</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178331">
        <property type="protein sequence ID" value="EFP90279.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334698.2">
        <property type="nucleotide sequence ID" value="XM_003334650.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L184"/>
    <dbReference type="GeneID" id="10530173"/>
    <dbReference type="KEGG" id="pgr:PGTG_16305"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16305"/>
    <dbReference type="HOGENOM" id="CLU_410570_0_0_1"/>
    <dbReference type="InParanoid" id="E3L184"/>
    <dbReference type="OrthoDB" id="2504869at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="239"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="312"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="450"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="109"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="320"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="343"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="466"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90279.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="669" mass="75505" checksum="447154212EB05F94" modified="2012-04-18" version="2">MTTNSSSSDSDSEPEHNKKNEICYDSEPERIKYFNENKNKNKSQQTTETTTTETTATTATETQPTHGNHEQPQTIISSNSDTDSIEIQPVNRLTNLISKFRHQPTPKQSSSSHNSRKSSSSLSSVSSHKRRSTKPAAAEQDQEPPLSIDYSTLLRLNRCPACDNPWLTYKAPRSKLNHIQRCARQELIPPRHLTLRIENLLQDQDINKGLIDRHATHQNLQTSDLQNSGQPIQSAIVVPRTSSTSTSASIHNLEKSSPSPPPPPSSSSKLTPKSEVFRSSTLKVLHQQTLDRNAQINTKNDQRSYSLWELASGDDDYPYDRSSAPSSPRSQRRVNTDRRRRTSDKGKKKLEDLSLSSSSSEDDDFDRLNNSDLFDHEEEQGYIEMPRYEQWTRSQLENESERLGYSDGPELSTSALVSRAQTAWTHQMAYPPHKMEPVAQEFNTGRSDLMGLRDGSNSIGSHPVDREDDDVDDEGGEQVGGLDEQADLDPYYQELEWENNLLNPQDHELFVESESVINPNPPVRPFFESFSKAKLELEYEKLVAQIQKALDLSTASSSPLQPLTDLPADLDKIPKTKPALVKKLLRLWDALYSPAHPSPSDDLVLHSSGSVPQPFNSSVTIRKEKLDLVGLNQRMILLFHSLDPQLYCRILRFEVGLFLFFPSILYAVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3L1F5</accession>
    <name>E3L1F5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_16140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178332">
        <property type="protein sequence ID" value="EFP90380.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L1F5"/>
    <dbReference type="GeneID" id="10530274"/>
    <dbReference type="KEGG" id="pgr:PGTG_16140"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16140"/>
    <dbReference type="HOGENOM" id="CLU_2005027_0_0_1"/>
    <dbReference type="InParanoid" id="E3L1F5"/>
    <dbReference type="OrthoDB" id="10276059at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP90380.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="124" mass="13962" checksum="A2F4F65CD15F2019" modified="2012-04-18" version="2">MDTKLGVNLVHKRLRLFLVRMSTELRDVDDALTQGSHVIHTVPEAGRSEVLCGIVPPPTVMRASLPHWYLRLKDSIASGIDLPLPEDWVEDCRRSMYANFISLSSKLAMLTGAPEDHDLPSKEW</sequence>
</entry>
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    <name>E3L1H6_PUCGT</name>
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            <fullName evidence="2">Uncharacterized protein</fullName>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178332">
        <property type="protein sequence ID" value="EFP90401.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L1H6"/>
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    <dbReference type="KEGG" id="pgr:PGTG_16161"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16161"/>
    <dbReference type="eggNOG" id="ENOG502R0PJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072137_0_0_1"/>
    <dbReference type="InParanoid" id="E3L1H6"/>
    <dbReference type="OrthoDB" id="2506660at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="263"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="289"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="321"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90401.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="333" mass="37674" checksum="DBA8CC6FD93993E0" modified="2012-04-18" version="2">MADLDSDPNAEERGRFGHIFKSDKKVQQEVKLWAEGVQLKLKELGDQFGIEGFLVLAGQEHRKPFFFQGGSFYGDEYLRGLVDEGDPIREFAVWTAGSKKTIKRRKKNKSNPAAAQPGNIIADQPPKNNKKSASALANRDVCQGGLAQNHSYISQELGKMYIEVQENKVKKDTHWPGTNTATKLAFCNRKLVIHENPVGLSAEHLVNVPVKKMDIETTWLVLRGLKNHWIELVEHQFDVAPKKRRVCKQKAAAIESPTQEINADKTNVASKSTVPQKRKASKIKSATQEIDDETEEEEEEETDDEDEEDEDELISNSDFFDNSDNDEDEDEDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>E3L1H8</accession>
    <name>E3L1H8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_16163</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
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    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transferase hexapeptide repeat family.</text>
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    <dbReference type="GeneID" id="10530297"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16163"/>
    <dbReference type="eggNOG" id="KOG4750">
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    <dbReference type="InParanoid" id="E3L1H8"/>
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    <dbReference type="Proteomes" id="UP000008783">
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    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="CDD" id="cd03357">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001451">
        <property type="entry name" value="Hexapep"/>
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    <dbReference type="InterPro" id="IPR018357">
        <property type="entry name" value="Hexapep_transf_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051159">
        <property type="entry name" value="Hexapeptide_acetyltransf"/>
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    <dbReference type="InterPro" id="IPR024688">
        <property type="entry name" value="Mac_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23416:SF23">
        <property type="entry name" value="ACETYLTRANSFERASE C18B11.09C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23416">
        <property type="entry name" value="SIALIC ACID SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00132">
        <property type="entry name" value="Hexapep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12464">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01266">
        <property type="entry name" value="Mac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00101">
        <property type="entry name" value="HEXAPEP_TRANSFERASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RRM" evidence="5">
        <location>
            <begin position="291"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="221"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="481"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="225"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="240"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="534"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="562"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP90403.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="589" mass="64276" checksum="4F5E9E8197D7C455" modified="2012-04-18" version="2">MPPKHTPTGTRTNQSVATTMTENEKMLTGKAYLASDPQLVKARLRARALMQRYNGYPWPTYEADDPDPDYFGPDDRRALLAELFNLQLDDLKQKPLEIEPPFYCDYGTNITFKGPFYCNFNCHVLDCAPVVFGSRVICGPNVQIYAGTHSTEICERQKGLERAYPVTVGDDVWIGGGAILIGPCTIGNGTTIAAGAVVRGDVPANVVMAGVPARIIKHLNSNTNKSDHESNSDLKPGHPKSSTSNANQSNHSKSIAARSPGGGSKNLNPTSVATDDDQASGHSEETSDPFAECFLANIPSALSNGDLHNALSVFGEVRSLTMDRSRRLGFVRFGSAQAAEEAVEAGRGPDGLVVMMNQETNEKMVVGIERRRMSDTNRTFNPDLITSTRCLIRRLPNTVDFEELKRTIEEHAGSLVEWTVQDFTSPDPTKPSVDKFISLEFEQLCGAREAVRLSQNINGTLGGIDVPGTDGIKARIEARKQFSPQSAYRTSYRGHHQQPFHPHSGGGYHQTYPNHHQQHHPRSSRGSYGGYHSRGGSPNHNHHYLTTTTNPAPGTGGGPGNYQNNKAFNGSFTKYKPRRSVPGGMNGSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3L1M3</accession>
    <name>E3L1M3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178333">
        <property type="protein sequence ID" value="EFP90448.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334867.2">
        <property type="nucleotide sequence ID" value="XM_003334819.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L1M3"/>
    <dbReference type="STRING" id="418459.E3L1M3"/>
    <dbReference type="GeneID" id="10530342"/>
    <dbReference type="KEGG" id="pgr:PGTG_16035"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16035"/>
    <dbReference type="eggNOG" id="ENOG502T93X">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052858_0_0_1"/>
    <dbReference type="InParanoid" id="E3L1M3"/>
    <dbReference type="OrthoDB" id="2498386at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90448.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="400" mass="45288" checksum="C1A21970CD304BE1" modified="2012-04-18" version="2">MMTNSQESAISGLIDSFSSLRNEDSSEQVRHYETLVTEELRRVHDAYRVLWDGIINRPDPLALFNQVEIRTELLDQLQSKRLPTLQRQVVSLSNALLGRSDLQAKPVSKLQLILKSLSKLDATLGKIKFAIACISPSVDPEVIRDDKDFKKLKAFTCSRLALRIYEVTGRICKLLETSRRFFEKSGHAFENEPRKKTKVLAIRDACSDSIDKAIKFMNKSELNHIQDGWQSSINTVDVSLESFLDFVNRSPHSIEESHSIPDQEPPRSGKTSGQDHPGLSRATISVISIMKLIRLFLAKLIKASEDKETFRMVTNLSSRELEICITMPNNLSEAIKSLVQGLSEDPGDDRLEGIMVINQAIHHLISTPRAVLFMVDYVFVHLFNPANQPSPKIYYKAWVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>E3L204</accession>
    <name>E3L204_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">PNPLA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_16605</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PLPL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="DS178334">
        <property type="protein sequence ID" value="EFP90579.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334998.2">
        <property type="nucleotide sequence ID" value="XM_003334950.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L204"/>
    <dbReference type="STRING" id="418459.E3L204"/>
    <dbReference type="GeneID" id="10530479"/>
    <dbReference type="KEGG" id="pgr:PGTG_16605"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16605"/>
    <dbReference type="eggNOG" id="KOG2214">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009031_2_1_1"/>
    <dbReference type="InParanoid" id="E3L204"/>
    <dbReference type="OrthoDB" id="15478at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004806">
        <property type="term" value="F:triacylglycerol lipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006641">
        <property type="term" value="P:triglyceride metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07232">
        <property type="entry name" value="Pat_PLPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050301">
        <property type="entry name" value="NTE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002641">
        <property type="entry name" value="PNPLA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021771">
        <property type="entry name" value="Triacylglycerol_lipase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226">
        <property type="entry name" value="NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14226:SF66">
        <property type="entry name" value="TRIACYLGLYCEROL LIPASE PTL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11815">
        <property type="entry name" value="DUF3336"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01734">
        <property type="entry name" value="Patatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51635">
        <property type="entry name" value="PNPLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 5" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4 5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="212"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="525"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="domain" description="PNPLA" evidence="8">
        <location>
            <begin position="387"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="40"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="132"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="722"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="842"/>
            <end position="881"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="GXSXG" evidence="5">
        <location>
            <begin position="418"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="40"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="147"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="164"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="186"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="862"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="420"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP90579.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="881" mass="98999" checksum="4647369C319E7A35" modified="2012-04-18" version="2">MEKEHVLPDTRQPINKAYIHTTQSSRAHKHSFPKTHLKTVDGRETADIARDYNPREDRARLEEKREREIQRGMTTRGQQAPYGEIDPDRLSPPQGEAGWGCDEFKKKWYNDDDLLVFRQALHAQDVDLHHLPVGPHSFPARPPPGSSAPSSSQAGSTDPTRLHPSSSSSSGRPSSHADPAPPSSSSHKRASKNPKPRKDIVREGWAYHISRWPLLTLIGVAFMFEFLIYVSVRQIVRLFETLMSWRAQEGRLRRKLRAAETYEEWKAAALELDDLLGFGQWKRNASNAYYDSISVRKVVASLERSRLNNDPESLKGVLEVCLRANFAGIESIRLYSQTHLGTKTLIENYVDEVEKSLVYLRETTHLSAQEKTVFFRRAAKNMGTTALCLSGGATFGFYHFGVIRALLDARLIPTVITGTSAGALVAAFLCTHTDEELDRLLVPEIADMITACEDPISVWLPRMIKTGARFDTVLWAKKSSFFTMGSMTFLEAYERTGRILNVSVIPHDVHSPTTLLNYTTAPNCVIFSAILASAAVPLVLNPVVLLEKSKDGKVRPWQFQGKHKDGSLRVDVPLESLHLYFNTSFSIVSQVNPHIHLFFFQPRGAPGEPVVHRKGKGWRGGFFLSALEQYMKIELIKNLRVIRDLELLPLLGGQTFTAVFLQRFEGTVTIWPHSRFRDWFNILTDPDRKELARMINVGKRVTWPKIRMIENRLRIERQVFMGRQETKRSQEPTIPNPQGAAVSGDGGGGGTEGSQDEMGGISGVENERSQVYGTDSEVEMPQMGSPEGNLRQLRMLRERRNLHRRLSQSSKSVGLFLDQLVGDDTGGGGNSSAGEEIVARKSFRHRHSSASIRSARLVNHSDGSDERDRNESTAEELLIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3L225</accession>
    <name>E3L225_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Response regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_16626</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178335">
        <property type="protein sequence ID" value="EFP90600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335019.1">
        <property type="nucleotide sequence ID" value="XM_003334971.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L225"/>
    <dbReference type="STRING" id="418459.E3L225"/>
    <dbReference type="GeneID" id="10530500"/>
    <dbReference type="KEGG" id="pgr:PGTG_16626"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16626"/>
    <dbReference type="eggNOG" id="ENOG502SFN6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_490137_0_0_1"/>
    <dbReference type="InParanoid" id="E3L225"/>
    <dbReference type="OMA" id="THDESAY"/>
    <dbReference type="OrthoDB" id="2499189at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17546">
        <property type="entry name" value="REC_hyHK_CKI1_RcsC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050956">
        <property type="entry name" value="2C_system_His_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719:SF28">
        <property type="entry name" value="PEROXIDE STRESS-ACTIVATED HISTIDINE KINASE MAK1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43719">
        <property type="entry name" value="TWO-COMPONENT HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="4">
        <location>
            <begin position="22"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="395"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="481"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="410"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="421"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="481"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="2">
        <location>
            <position position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP90600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="556" mass="61650" checksum="618EA25DB6BCCEA8" modified="2011-01-11" version="1">MSPPTSIYTTIHQPTTQQVKLKVLIVDDNLLNLRVMKQLLKTKLADFLDLDSLQTADSGFEALDLLRNNDFHLLFLDISMPGISGLEVCRRLRNDSRYSSSKLHICAVTTDLADWQVQLYKSIGMDGVIGKPLKNLDLEFALRACLDSVLYQNHLETLLPPAFYFRRYGSQQLLPDILLSPIDAPSDPVVSLHSLSTITSTAPLLTSSIGLQPILSCDDQRKFRQQSVVSDISVAGVLAYEEPSTFRRIPRPCLGQLDLVDETLISACRPCFDSFDTSSASGSIITCSSSGPNSATAIDPLSPSYCSEDEPFEHSKRFPAFPTAPIITSTHVPLGLRSPRRWSSPLYSVGSPLADAQLTQEEKDVFAAGFQAAMNNMAIDQACSQWGENCLSATTPSANESRYGNLEPARSSTTQNMSNWADEDDEADEERLEEQEEEEDEQDSYRWARLAGPISCEDTKLSSVTRQLISRRSHPMLRLNNLDRTRTSEAEPRPVELRPTPEPDSPFMRKSKSAAGLPIRPSWQLVQLSAINYQSVLKSGTLLDEVLFGLDLEDSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3L339</accession>
    <name>E3L339_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17236</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178339">
        <property type="protein sequence ID" value="EFP90964.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335383.2">
        <property type="nucleotide sequence ID" value="XM_003335335.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L339"/>
    <dbReference type="STRING" id="418459.E3L339"/>
    <dbReference type="GeneID" id="10528379"/>
    <dbReference type="KEGG" id="pgr:PGTG_17236"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17236"/>
    <dbReference type="HOGENOM" id="CLU_070862_0_0_1"/>
    <dbReference type="InParanoid" id="E3L339"/>
    <dbReference type="OrthoDB" id="2571149at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="359"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90964.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="385" mass="45595" checksum="67B70C22DE329C85" modified="2012-04-18" version="2">MTILIELNAHQQTPWPSNMSTTTTATTSKFILPHFGPRKLTPLKSSIKPTDIPPETTRFLSQVYTIIQNRPREFQRTQVNRPRHWWRSSEHRTPTRWSLYRHLLKHSSWFDNQLISKSANPQPKSATFSSTPVLTNQIRSEFKRYKTLRTIPHTQEVLRQGYLLLTKLVNAKFGDLDDLNELKSYRHDLVQSRYRKIWGKTYKAQLNAQRPRTPIMTGRFLRPSVLNGPLPRLAHQPLHISMMMSSRRKKRERRVAEHRHLKDKLDTVRQEHQFDSAIIAHDQAHVFDQEHNREVKGILDRLNDISHSFTLDKERERKVYSRELLDKIEAARRRRPGVMAERNRWKRIKTRTAELDYVVEDDPEGGAETAEGKRFRLPEPSFWQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3L378</accession>
    <name>E3L378_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17275</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178340">
        <property type="protein sequence ID" value="EFP91003.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335422.2">
        <property type="nucleotide sequence ID" value="XM_003335374.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L378"/>
    <dbReference type="STRING" id="418459.E3L378"/>
    <dbReference type="GeneID" id="10528500"/>
    <dbReference type="KEGG" id="pgr:PGTG_17275"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17275"/>
    <dbReference type="HOGENOM" id="CLU_023058_0_0_1"/>
    <dbReference type="InParanoid" id="E3L378"/>
    <dbReference type="OrthoDB" id="2512510at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="373"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="443"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="255"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="443"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91003.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="588" mass="64592" checksum="D72189CF46FB89CC" modified="2012-04-18" version="2">MLSHHQTSPHDYPAGLVTRLIPSAPLHQVLPVNDQQPPIQPPADIPTPSPGGSSVPFTILANGGVRTWKPKRTAEEMQLANLIAAEKRARRFQNQADKAAEARQKHATIAANKILKAQKAELTTPWVTWTEEQTIELLNFVRMVKEDHSQVKGGFIPFGNYFAAYTGRPEAFPLLQSFSTATRLSKYRALMDKWKKVKDVVDRSGAGGLSLALEAGGVSQEVWDLILDMHGDNPAATGEGLSSSYADYETLLEESPPSVSSGNSGSLDVNEPNSLPTTPAPQKKRLTKYQRLSAGLTPAKLALDPDLESDTDLPAELVLGPPGTSTGLAGLQGTVPTAVVGGSVANIPGSPATTPLVPRKGTKIKVKLGAAKNTISRHPAEGSSIPQQRGRTKEKSHNDASGTGMLVMMQKAQEATASWAAEEHRDALVERARQDTLRAEERANRQRLKEAQADERAAELKRQDELRADDRRAAEKRADAMEVTHRQEMQEAKEDRELLRQQAKLDREVADHQMKMEREGAEDRKRERQRYLDQKAEEKKEAQERKDWAKRQQQSWQMFELAMLKAMGFAVANPDDAPPAGGAGSSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3L4M4</accession>
    <name>E3L4M4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_17749</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178347">
        <property type="protein sequence ID" value="EFP91499.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335918.2">
        <property type="nucleotide sequence ID" value="XM_003335870.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L4M4"/>
    <dbReference type="GeneID" id="10529500"/>
    <dbReference type="KEGG" id="pgr:PGTG_17749"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17749"/>
    <dbReference type="HOGENOM" id="CLU_162855_0_0_1"/>
    <dbReference type="InParanoid" id="E3L4M4"/>
    <dbReference type="OrthoDB" id="10277795at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP91499.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="115" mass="12987" checksum="18120003CFFE8532" modified="2012-04-18" version="2">MVAFLSITQSNAYNCDNAFQRIPNTPNSVVCQTGLVALSDWIFSDCVTTPAGWVKNHVRVKEFTVNIALHRIEIWGHKFWPGTFVPALDSEPYHCPMSHENLRRATCPNDQCVPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3L4R4</accession>
    <name>E3L4R4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">HORMA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_17593</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MAD2 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178348">
        <property type="protein sequence ID" value="EFP91539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335958.1">
        <property type="nucleotide sequence ID" value="XM_003335910.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L4R4"/>
    <dbReference type="FunCoup" id="E3L4R4">
        <property type="interactions" value="742"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L4R4"/>
    <dbReference type="GeneID" id="10531476"/>
    <dbReference type="KEGG" id="pgr:PGTG_17593"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17593"/>
    <dbReference type="eggNOG" id="KOG3285">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072097_0_0_1"/>
    <dbReference type="InParanoid" id="E3L4R4"/>
    <dbReference type="OMA" id="WQFDVEI"/>
    <dbReference type="OrthoDB" id="1806at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000776">
        <property type="term" value="C:kinetochore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007094">
        <property type="term" value="P:mitotic spindle assembly checkpoint signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.900.10">
        <property type="entry name" value="HORMA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003511">
        <property type="entry name" value="HORMA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036570">
        <property type="entry name" value="HORMA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045091">
        <property type="entry name" value="Mad2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11842:SF11">
        <property type="entry name" value="MITOTIC SPINDLE ASSEMBLY CHECKPOINT PROTEIN MAD2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02301">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56019">
        <property type="entry name" value="The spindle assembly checkpoint protein mad2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50815">
        <property type="entry name" value="HORMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HORMA" evidence="8">
        <location>
            <begin position="45"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="138"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="149"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP91539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="268" mass="29935" checksum="B170D8381BF302C0" modified="2011-01-11" version="1">MSSVPTSRRTAGGTATRVRPARTETANKTTATDTTTKTKKRINLAGSTAVISDYFHYAINCILFQRAIYQQSDFKMVKKFGLNMMIVEDESIAEYLKKIVDQVRVWLIEGKITRLVLVIQQKSDGETVERWEFDVTVEEPPIDDPNNVSQPSSQAPASSSSKKDKQAGSKTKSKSLAEVQLDIQEIIRQIISSVSFLPIPTEPRTFKVLAYTHSVGQDEATEWNDSDAFDITERAERVSPPPSTPASYSRLSEKLKLIIPDARFFVIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>E3L4Y2</accession>
    <name>E3L4Y2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_17661</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178349">
        <property type="protein sequence ID" value="EFP91607.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336026.2">
        <property type="nucleotide sequence ID" value="XM_003335978.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L4Y2"/>
    <dbReference type="GeneID" id="10531544"/>
    <dbReference type="KEGG" id="pgr:PGTG_17661"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17661"/>
    <dbReference type="eggNOG" id="ENOG502RKU7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1143027_0_0_1"/>
    <dbReference type="InParanoid" id="E3L4Y2"/>
    <dbReference type="OrthoDB" id="2495203at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051654">
        <property type="entry name" value="Meroterpenoid_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35897">
        <property type="entry name" value="METHYLTRANSFERASE AUSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35897:SF1">
        <property type="entry name" value="METHYLTRANSFERASE AUSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP91607.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="243" mass="27028" checksum="0344FC868BD730D6" modified="2012-04-18" version="2">MDQFGAGMLEMPASADGIGAAYPLDDPRMSEELKFYQAATGIQDPQELKALLFEIRAEACKTFPFPCVLSRHPFFPEVLKAIKEKLTTRRIFVDIGAGMGNDLRLLIHHGWKRDDVLGVDVNSEWSALGYKLFRDRNLPVPNFIGNILQPEVLDVSTPAEFGSAESIDLRSLKDLNPLKGKVNMMSLNQLFHFFDEADQKRLAERCALLLSDEVGSAIFGMQDFCPVSGGRSSLTIDETKTDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3L652</accession>
    <name>E3L652_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17858</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178356">
        <property type="protein sequence ID" value="EFP92027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336446.1">
        <property type="nucleotide sequence ID" value="XM_003336398.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L652"/>
    <dbReference type="GeneID" id="10531981"/>
    <dbReference type="KEGG" id="pgr:PGTG_17858"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17858"/>
    <dbReference type="HOGENOM" id="CLU_045287_0_0_1"/>
    <dbReference type="InParanoid" id="E3L652"/>
    <dbReference type="OrthoDB" id="2498281at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="314"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="514" mass="56741" checksum="BA31DC3B12B9CE9F" modified="2011-01-11" version="1">MEPSDYSDYLDGLQSLDQVSNWLDDQLQPGGASLGSQRRSESVANTSSNTQRGGIDPKDEADNDNDGKDFKVAIEYKLFLTLKKNKSKPTNTRAGNKQKAATADAPDKYTRLVSKSNKLSLTWNASNTSLAVLKEAVLEFLRVLEPNQVFQLADKQEREKPLLNQTVLDNSKIFARFLEVAEEKGEAKVYLVQNDPKTIAERNEALKKLSEQDKENSNDQSVQAEPTAGASFNKQVIENMWLLRATHMPSENLTGSMELPVMIDPEDRKRFIALSPDRITLWARAMATNPEVTVDNPPKSPAFRFQTKAEFKGIGAGANKEGDNSINVGARTTQDLPSLPRTPGSNRSPAGIALAGGSTNPTNVSPILMAMGAYPPAMSWPPASPWGWGPPKPHFYAPYGGMPLETPPVWQPNTFITHNPHLAAGTMNTAPVSSPPASDDTVDINDYLSFCHINHHCESVQKALVDYGITHYQEFENIKPEELELLGVKKSKARLLVSNTKKYGRTLKERRLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3L7R3</accession>
    <name>E3L7R3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="7">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_18720</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="7"/>
    <dbReference type="EMBL" id="DS178367">
        <property type="protein sequence ID" value="EFP92588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337007.1">
        <property type="nucleotide sequence ID" value="XM_003336959.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L7R3"/>
    <dbReference type="STRING" id="418459.E3L7R3"/>
    <dbReference type="GeneID" id="10538747"/>
    <dbReference type="KEGG" id="pgr:PGTG_18720"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18720"/>
    <dbReference type="HOGENOM" id="CLU_011478_1_1_1"/>
    <dbReference type="InParanoid" id="E3L7R3"/>
    <dbReference type="OrthoDB" id="2507066at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="10">
        <location>
            <begin position="121"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="11">
        <location>
            <begin position="348"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="788"/>
            <end position="822"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="705"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="798"/>
            <end position="810"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP92588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="822" mass="92805" checksum="A83C45874573CBB2" modified="2011-01-11" version="1">MNQSETEIPIEDDSSHEEDSDSGQRISASNPIEDDSSNEEDSNSGQRITESEVIDEIAALNRGESLPDELASLKLQATSKKRVTLRQDILELNHQELKEHIRVESRNLYGDEAKDEQLEAVAALVHDRHTFFLAGTGFGKTRIAEMYHNLFQPYQKSIVLVLNPLDSLGDNQVEEKKKVGVNNRHIKAVNLTTNVLDAKMARKIISGEFEFVYLSPEALLNNEIFRDVYFNPKFQSRLSLIVVDEAHMVYVWGLVANGQSKGLTSHLKHGERGVFRPGYGELAARLMATNGTPLLMMSATCRPIAINSILESFKLTRQMVTFVEAELTRPEIRMLRIDMQKSLASSEDLADLYSTQEQTPDNEIIPTLIYSTTRNLTGQVLDVVNDAREANQEDNPFSTFARRYHSITGDMDKSDVTGDFTKGVFPVVSSTMALGLGQNWKRVRCVIHMGRGDPASICEMLGRCGRDGKKGLAIMFVEPHRKNGKNSVSDFTNVELQNDNDRMDALAITPVCLRIAFSLDNRLGYIPLSFDDPNYKREAKREVDAGFSECSCSNCKPESSKWVINNFKRANINNFDSFISDSPQDITELFPIRQEKQVADNQINWVEESGKKPLHEILEAFAQALVRYFCDFFDSEMKGAAAYPPDAYFSIIHARKISRNIKHLTLDNIEQLIGNEMLTGQFKMLFDHITLFQSTNSYQYISHRQEELDAELDSKAARLRKEKEDEDNQRAETTRAIQEAAAQKKIDTAARVKANAEKKCTAKEAKFIESKKKKAKRESDMAFLHQLQAQAEIDRQNTRPNASDPETSKSVLVDESMDPALL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3L7Z4</accession>
    <name>E3L7Z4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_18184</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178370">
        <property type="protein sequence ID" value="EFP92669.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337088.2">
        <property type="nucleotide sequence ID" value="XM_003337040.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L7Z4"/>
    <dbReference type="STRING" id="418459.E3L7Z4"/>
    <dbReference type="GeneID" id="10539207"/>
    <dbReference type="KEGG" id="pgr:PGTG_18184"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18184"/>
    <dbReference type="eggNOG" id="ENOG502RZND">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_413394_0_0_1"/>
    <dbReference type="InParanoid" id="E3L7Z4"/>
    <dbReference type="OrthoDB" id="2587928at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036426">
        <property type="entry name" value="Bulb-type_lectin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51110">
        <property type="entry name" value="alpha-D-mannose-specific plant lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP92669.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="664" mass="71313" checksum="6D16F95A473BA157" modified="2012-04-18" version="2">MTNSPVVVRRAVRPEDLPPAFVNRPAAYLSSLFENGGPGTVVLLAQGSIWELEAILKIAVNDAELATEGYPTDPNLHAQVHSVGEGEATAIFFHNTSHVKLSHLTIDGRRPDKGWVDGGGPLIACGGREGKDPVVQYCVIRHPRGWSSLQVFDNCEGGRVIGNKIGPAGLPAPKGPWADGLSIACRNGLIANNEIVDATDGAIVLFCAPGTMCIGNTIIADKQNLLGGINMVDMGPYSCDYTDTRVFNNVIKSTGAHIKLGIGIGPLAWCPTWNENTFGGKVIDNTFGPGRFGYAIGMSGCRDFEVVGNRVTAGTTFTGDLSGMQEPLNAPPMAFLKASQPGLVENCVIQQDFIEGRAAFLIGVEDRPARKFRFQGSQLNLTSTDGPIVLDRARISLETTGELRVLCNATSRVLWTSGSAGSVIGARLSLEDNGHLTIREAGTGKLLWDPVQFLEGCFQVGNQAALTVSDESPYLSLWSECNSLVWASEYVFGKGSFELAPNQFICICPTRTRAQPPPIPPRIGAVLDNISHAVHHPPPMIPARPLPPPAYIFLDPVTSNLVIHRGPHPHQPHGHVLWASDLFGHLPKQIASRANPGCETRCAFQGGDGNLVIYANPHDHQPEERCAVWASGTCCEKLLITYEAEQGVQIHFLDPQGVILKSIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3L9R9</accession>
    <name>E3L9R9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19062</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178388">
        <property type="protein sequence ID" value="EFP93294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337713.1">
        <property type="nucleotide sequence ID" value="XM_003337665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L9R9"/>
    <dbReference type="GeneID" id="10543526"/>
    <dbReference type="KEGG" id="pgr:PGTG_19062"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19062"/>
    <dbReference type="HOGENOM" id="CLU_030899_0_0_1"/>
    <dbReference type="InParanoid" id="E3L9R9"/>
    <dbReference type="OrthoDB" id="10329732at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="444"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="186"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="471"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="499"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="552"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="628" mass="69880" checksum="FD020AADD23912C3" modified="2011-01-11" version="1">MIICGPVRSNRQFEPCLAILKGEGFPSRILPPRDYCHTNLLSLLKLSLLSSLATSLIRSDYYTSDHSSQLACVHSKFAAICSELSTHHTFLCVATEIATIGTPFLPKFSLQTRMSTPSNKSVSTTAQPKQAPTTEPISASHPSKAPKKPAARVHKKAKKPISPEIVPSEWDTSSAEETKSDKPKAKSSTAPELKKSSTTADPFKSKKPIDQLIDKEVLKNIHIQKSTHALKAPVPQQAPSVISNQPNLAAVSTENRSVDPVVSKTAVEHEESKSTRPEKNFFAPSFPKVSSDSTIQKKIENYFVPQGRTTRSISSASSILPSASFTTEPYEPADSSDLDVITGATAQLWSKPKTSKAPLADDILRRYRPSFHPLLQAVKLQQEYYRRALETNDEDQKIVALRAASTAQTDLLRVMNLEEFLTLFEKWNPVAEYQDYLTGQTGKDYWKKEGIPTTPSQAPEDVMRPPEENQEQPTINSSDQPPAAMNHPQGGYYYPPTNPQAQQPYWNQPSYPVTAQGYQYHPPANYHHPTSGQAHPASATGGPTTFQSNQNRNRRRNSNRRRNQSHRGPDWLPRPGPNRAPQNSNAGSSREARRRSHQLSIHREMIRLNRTTQAQFQALESMRDQNAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3LA42</accession>
    <name>E3LA42_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aldehyde dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_19494</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178392">
        <property type="protein sequence ID" value="EFP93417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337836.1">
        <property type="nucleotide sequence ID" value="XM_003337788.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LA42"/>
    <dbReference type="STRING" id="418459.E3LA42"/>
    <dbReference type="GeneID" id="10537284"/>
    <dbReference type="KEGG" id="pgr:PGTG_19494"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19494"/>
    <dbReference type="eggNOG" id="KOG2450">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005391_1_0_1"/>
    <dbReference type="InParanoid" id="E3LA42"/>
    <dbReference type="OMA" id="FPEGCFQ"/>
    <dbReference type="OrthoDB" id="2497729at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004029">
        <property type="term" value="F:aldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07102">
        <property type="entry name" value="ALDH_EDX86601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000009">
        <property type="entry name" value="Aldehyde dehydrogenase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.605.10:FF:000012">
        <property type="entry name" value="NAD-dependent succinate-semialdehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029510">
        <property type="entry name" value="Ald_DH_CS_GLU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11699">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00687">
        <property type="entry name" value="ALDEHYDE_DEHYDR_GLU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="6">
        <location>
            <begin position="4"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP93417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="470" mass="51931" checksum="81E4546094CD02F5" modified="2011-01-11" version="1">MTGDKIITISPNTNQPILERVEPTKAELEGLLNNSLNAFKEYSKTTDLSQRQTIVKKALGLLSSKQDELARELTEQMGRPIAYTAKEIQTAVNRSEYLLKISDKIFADTPGEPEQGFKRYIRKEPVGPVLIIFAWNYPYLILVNSLIPALLAGNSVILKPSPQTPTIAEHFRSIFIEAGLPKDVITFFHSGGMHSKIPQIAIRSPLVKLICFTGSVEGGLAVQTAAAGRVVPVGLELGGKDPAYIRQDVDLDWAASEIVDGALFNSGQSCCSVERVYVHESICQQFVLAIQTVLKSYRLGDPFDPKTHLGPVISSQSRKVIEEQIKDAVREGAKDVTPENDSFTNLPAHGNFIKPTLLVNVNHQMRVMKEETFGPVIPVMSVKDDQEAIELMNDSEFGLTASIWTKDLKLAEELTQEIEAGTVFVNRCDFPAADLAWTGWKNSGKGVTLSQFGFDQFTKLKSIHLKSYPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3LA44</accession>
    <name>E3LA44_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19496</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178392">
        <property type="protein sequence ID" value="EFP93419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337838.1">
        <property type="nucleotide sequence ID" value="XM_003337790.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LA44"/>
    <dbReference type="STRING" id="418459.E3LA44"/>
    <dbReference type="GeneID" id="10537286"/>
    <dbReference type="KEGG" id="pgr:PGTG_19496"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19496"/>
    <dbReference type="eggNOG" id="KOG1297">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061714_1_0_1"/>
    <dbReference type="InParanoid" id="E3LA44"/>
    <dbReference type="OMA" id="IRCTSAR"/>
    <dbReference type="OrthoDB" id="197967at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019129">
        <property type="entry name" value="Folate-sensitive_fs_Fra10Ac1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09725">
        <property type="entry name" value="Fra10Ac1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="194"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="243" mass="27746" checksum="9BAB0D3F7266FDFF" modified="2011-01-11" version="1">MPESSAAVRHRSKNFYGRTPPSLPTCKTELDILIERHQFIRDCDEQNLSWEDALAKKTYDQLFKELAVCDLSRWKEGKVAMRWRTANEVVDGKGHFTCANLRCIHHELGLKSPPRSLSKKAHPLGAFANFEAQHSFIPLEEFSRAGERSELTPQIPLQELQTSFGYLEHGVRKLAEVQLNLCKRCAKKMNSCSLSKRRTENRAPTRALVHASRKRDLPEGLHSSTEREPCPERPAKIRCTSGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3LAE4</accession>
    <name>E3LAE4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_18933</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178396">
        <property type="protein sequence ID" value="EFP93519.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337938.2">
        <property type="nucleotide sequence ID" value="XM_003337890.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LAE4"/>
    <dbReference type="GeneID" id="10543634"/>
    <dbReference type="KEGG" id="pgr:PGTG_18933"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18933"/>
    <dbReference type="HOGENOM" id="CLU_2543683_0_0_1"/>
    <dbReference type="InParanoid" id="E3LAE4"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP93519.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="83" mass="9453" checksum="AFD029319312AD8F" modified="2012-04-18" version="2">MEGEGHKDITTSNRWGIDLANANRIEQIQAQIASYCSKKKVLSSLVPDALVIWNIHRVRLVRANWDPTTGCMDPLMERMDTKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3LAJ9</accession>
    <name>E3LAJ9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_19528</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178398">
        <property type="protein sequence ID" value="EFP93574.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337993.2">
        <property type="nucleotide sequence ID" value="XM_003337945.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LAJ9"/>
    <dbReference type="GeneID" id="10534782"/>
    <dbReference type="KEGG" id="pgr:PGTG_19528"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19528"/>
    <dbReference type="HOGENOM" id="CLU_028052_0_0_1"/>
    <dbReference type="InParanoid" id="E3LAJ9"/>
    <dbReference type="OrthoDB" id="2505591at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="190"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="451"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP93574.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="471" mass="53326" checksum="0F197270966CBF50" modified="2012-04-18" version="2">MSDRTFRESQPSSELQGRFNHPNHPNRPLMNSQLPEGRFAAASFNEQILSSFVSTGSAFVTPNSGVSRKTARGTELKKKRVRPDEDIKRWNRLRHKANSPFVASQDPENPIQSPSSQQLGPLKKKPFYYFIPPARQHDPEPDVMHTSQETLASSYKGLDHGTVIITPRGQEAFCKAKFIPFSSMPSDELLGWEKLVCFFLSRTHYIAPVKNNGPHMGGTMWADGWRKCSKACEAFGRYCSVTRLVAMMRKSNYVAEDEAVAVREANDWISVHLQELAPGVFEDYRATLIKNNLPSMAHMEWPPSSYHALDFASFLTFTMYDFFNETHFDSDANNWTLVCWIPILNPRTSDKDDPILADDGFDMIGGQFTFRDFQVYLDLNKVIGVTMCVFRSKDHRHQTLAGSSPSDKYTRIGFSCQMSEAMSNAVVAYINGTATGDCIAGQKKQIDNAEKSIVKQDQKQAEKQREREKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3LBM4</accession>
    <name>E3LBM4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_19879</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178421">
        <property type="protein sequence ID" value="EFP93949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338368.1">
        <property type="nucleotide sequence ID" value="XM_003338320.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LBM4"/>
    <dbReference type="GeneID" id="10535481"/>
    <dbReference type="KEGG" id="pgr:PGTG_19879"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19879"/>
    <dbReference type="HOGENOM" id="CLU_072864_0_0_1"/>
    <dbReference type="InParanoid" id="E3LBM4"/>
    <dbReference type="OrthoDB" id="2505500at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="46"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="214"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP93949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="330" mass="38209" checksum="73BB2D24F9637663" modified="2011-01-11" version="1">MAKSHKRSHSNYRPALTILLQPMRFFVMSLVKILALLLAHPGQTCTFLVVILAAVYFFNMVDSLVAFVLPFKPFRLLYSHIKSSSKNILDGLCGVVYPHRSSNAIPRLLKNTKDPLQLTTSVLVLLNEPGNFFAETTDLLELSNSLLFMNMKLGNHTKISSQVRNLANMYSLFTDEIEKLDSAGIRVLEIISQEYYKVIYQNKHPKNQVGESLVKLSNDEVENLQANINKTLELAFNLLNEQDKTSILFHEETSFLQNVRNRRGLWGFKKRGDKKVEAEETLIYLNHHRENLKRSWTTLEKQRRQLILFLDKIQNMKASFSLIRIHNFIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3LBT6</accession>
    <name>E3LBT6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_19958</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178427">
        <property type="protein sequence ID" value="EFP94011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338430.1">
        <property type="nucleotide sequence ID" value="XM_003338382.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LBT6"/>
    <dbReference type="GeneID" id="10535624"/>
    <dbReference type="KEGG" id="pgr:PGTG_19958"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19958"/>
    <dbReference type="HOGENOM" id="CLU_1918099_0_0_1"/>
    <dbReference type="InParanoid" id="E3LBT6"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP94011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="132" mass="15800" checksum="24706224B675F926" modified="2011-01-11" version="1">MGGENWLGFGYYDELLRDDLSQENVEYREGHKFLFNLCGKYNIPRSVIESIYGNISRRHCRTWMAWNSHCTELINWSRKYFESEEINPHNILQEWSSLINLKIKNNCVRRDCCNKKSCNSWMLSKMNLLILP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3NXF7</accession>
    <name>E3NXF7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UDENN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_20185</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DENND6 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS989935">
        <property type="protein sequence ID" value="EFP94256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338675.1">
        <property type="nucleotide sequence ID" value="XM_003338627.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NXF7"/>
    <dbReference type="GeneID" id="10527346"/>
    <dbReference type="KEGG" id="pgr:PGTG_20185"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20185"/>
    <dbReference type="HOGENOM" id="CLU_423971_0_0_1"/>
    <dbReference type="InParanoid" id="E3NXF7"/>
    <dbReference type="OrthoDB" id="10265409at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055037">
        <property type="term" value="C:recycling endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024224">
        <property type="entry name" value="DENND6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037516">
        <property type="entry name" value="Tripartite_DENN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13677">
        <property type="entry name" value="LD41638P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13677:SF0">
        <property type="entry name" value="LD41638P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50211">
        <property type="entry name" value="DENN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDENN" evidence="3">
        <location>
            <begin position="1"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="407"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="413"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="428"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="446"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="489"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="513"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="567"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="605"/>
            <end position="614"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP94256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="646" mass="70075" checksum="5DC5139F71E86186" modified="2011-01-11" version="1">MSWPDPTPGAELELTFLGQDHKFIIPLPNQAQIPDPKTNFPLLKAASGSLTGPQSLQYMNPLDYHVLASNPTTYLSYILFGSNPNTSSSSSSPASSALNSSAVSTLSVSDLWLLWEILILGEPLVVFGATPDIVSDTILHLKNLIRPIPFHANWRPYITIHDPDFGLLFGKNKSRAVGLIGATNPIILTATHAWPNVLSLSKQGASLNHHIGLLTDRKRVLHKNKSVVKQLLLCLERKDYHHADIVISRHFSVLTEQFLQPLQRYFTTLLPTAEGTGTEPRRTGTFNSELFLKSLKEHTTALEFRSRLMTGFTASSVSAPGFYTAFLRSPNFSAWLHRQLVLGNRAVRDRFFSNSNQLNKQPKSRPVSFSHSEELNDIDIPSVNSLPPFVSNDPFQQVIKKLHNQLSKKPVPTGLPPIPPTGQPIEGPTERSKKEYPKVGEGSPSGGSQAHSHSYNNNQRSPSEGGVLLSSKKNTPGLGLQGLKKSRTIGGGARAGGEGGGGDGGGERKMRRSSSTMSNASSSNTTTSSLTSRRKPGGLDGQPADDGLSGHTNSRRSRRRRPDSLHSSASPTTGGSSSPTSLSRPELADSAAVHHLSYPNPAPFDHSHPSDNRPLRSSSHPYPNLPDHHPHPVAVNDSLISLSHPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3NY48</accession>
    <name>E3NY48_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_20453</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS990030">
        <property type="protein sequence ID" value="EFP94497.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338916.2">
        <property type="nucleotide sequence ID" value="XM_003338868.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NY48"/>
    <dbReference type="GeneID" id="10527792"/>
    <dbReference type="KEGG" id="pgr:PGTG_20453"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20453"/>
    <dbReference type="eggNOG" id="ENOG502S2AH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011407_4_0_1"/>
    <dbReference type="InParanoid" id="E3NY48"/>
    <dbReference type="OrthoDB" id="2504373at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041320">
        <property type="entry name" value="CxC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040521">
        <property type="entry name" value="KDZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18802">
        <property type="entry name" value="CxC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18758">
        <property type="entry name" value="KDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxC1-like cysteine cluster associated with KDZ transposases" evidence="2">
        <location>
            <begin position="137"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="942"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="945"/>
            <end position="966"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP94497.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="986" mass="114096" checksum="A74990FC738DA4D5" modified="2012-04-18" version="2">MTRRKTTKDPPGTRSERLRRSQNATLNSIAWARLRRGDRTGNQEIEQMEDTEMMDNPYDPNQESEWEDEGPDSSDEDQEDHTRWVTLDEFEDQEEGIDPTIPSAQEEYRRLAKEYNWNRLLKELHTWYVTLKLHTKNWSGPNSYEEHKSDSCRCSSRDQRSRPVDMVDIYAQRRRLILFCKCTHDAVRLLRQGYLAGSPIKPQTAFSLPLLIFHNSLWNNCHIGMLPFTVALTEFLEPRSERLCAKGKNHARDMRKPFSAAVDLFRLLQERSDDLMDSTLGLTEQDKLAGRSCPACFGPEPANSMDYPDSVRNRLIVCLDGNFQHRHHSKASRDYETLQTPSIFLPEGAVEMMTQEIRHMEVMNKTPKQADRCADAHKAADDKRNESTWKGCDDTGLMGLCCRHDSAVFLANIYKSGELRALPLALMKRLLRLDPDRPVGILYDIGCSLDKYINLRGLLPDLTKETTFGTSIFHSYVHNWACQLDYNPRLNTGWGLSDGEGLERMWSYLSPLVSPLRYATRNHRLSAIAHRLKYHNTRGIRQLPHWLSRKFKLASKRRIETRNELAKLLSAQNPFKRPGCKYTISYFQKQWNHQRTFRADHTDEEQERRDKLIKIYEHQLNIEILRQEVKKITDKIERVSKKLSKDAAEAEALGIGLPSGDENSDEQRLLLLLWNSKNELYIQAVQLRAERQPLLDANKLGTPLGTELKEKILKAMANRRPAVNKLLDKYNKLFAEYLEKFPDQQATDTSHYPVTYDDFSDWPLDHQFWNDGLYFQSKAPWAIEPDVRAGINYVLILNRVQEEFQLIAQELARAVGWAICYYIHVENTITALGERIKLLEDRPDDVELDRFDNLVLYGLNRRNKLRLIRKELRCRLTLHATLVEEWHPHVLWLANHCQPSEHCKVLLNDWDNIRKKIESEKGGVLASQPEVDVPLEEAVLGMGADDGEDADENMMSETEQANDDDASNNPIVVETAAGNNSDDDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPN7</accession>
    <name>H6QPN7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">OTU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_20836</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EHS64139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890548.1">
        <property type="nucleotide sequence ID" value="XM_003890499.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPN7"/>
    <dbReference type="STRING" id="418459.H6QPN7"/>
    <dbReference type="GeneID" id="13542962"/>
    <dbReference type="KEGG" id="pgr:PGTG_20836"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20836"/>
    <dbReference type="HOGENOM" id="CLU_809269_0_0_1"/>
    <dbReference type="InParanoid" id="H6QPN7"/>
    <dbReference type="OrthoDB" id="415023at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22744">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050704">
        <property type="entry name" value="Peptidase_C85-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419">
        <property type="entry name" value="OTU DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419:SF7">
        <property type="entry name" value="OTU DOMAIN-CONTAINING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OTU" evidence="2">
        <location>
            <begin position="206"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="91"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="110"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS64139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="343" mass="38130" checksum="62830EC53F0D7B7E" modified="2012-04-18" version="1">MASNIPTNLVAAGKPTLVNTGKQVRSEDQSAQKTPKESQVDSEAKDVPPCVVVDLTQVDVEVPRHMAIDLTMEDDVQAPAIRIVNSLMPLAHHPEGDGGGSTHGDVTHHSPSSNDIDLSSDSNTKEEPPQEAPIDLTGDSSSEEIEIEMEIEIISQGRRLSPQETWDRIKSDIATYVEQPNLYCIPTVGLKKAQEYIVESNLTIRYTTGFIGSDGNCLFRAFSQWQNGHQDDHDKLRDEIHKYSKSQRGLIKNYLGRDAQDEHVDRWINKMGQLGVWGDSMALQLLANRFGVILFVVSYKPPDLRGHAREYMPGNVPAGQPTPVYFIVNLPQHFEIIDPFPNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQ79</accession>
    <name>H6QQ79_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21053</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EHS64794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890315.1">
        <property type="nucleotide sequence ID" value="XM_003890266.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQ79"/>
    <dbReference type="GeneID" id="13542193"/>
    <dbReference type="KEGG" id="pgr:PGTG_21053"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21053"/>
    <dbReference type="HOGENOM" id="CLU_215067_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQ79"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="61" mass="6743" checksum="782CB6196887036D" modified="2012-04-18" version="1">MDSTDDNPSANTKEPDEPTYLNRSSLLERLSSPIAATLANKNPLSKPISEQLNMRISLLDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQE4</accession>
    <name>H6QQE4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EHS62556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890269.1">
        <property type="nucleotide sequence ID" value="XM_003890220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQE4"/>
    <dbReference type="GeneID" id="13541175"/>
    <dbReference type="KEGG" id="pgr:PGTG_21104"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21104"/>
    <dbReference type="HOGENOM" id="CLU_1338086_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQE4"/>
    <dbReference type="OrthoDB" id="2503851at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="205" mass="23845" checksum="F6460F062D0EB4C9" modified="2012-04-18" version="1">MPRPPKKKAKKNAENKDDFRSKSGKASDLEAESSDEMKNLASIVKLKRSSKTGNHSFEIFERKCSQLIENTTKEIELKIKTHEENLARIYESVEEINEEVEPGENEAVFERKLNKNFDAEINSLSRALTDMNTQKSCDRDELQVDSELLEELNLRPERLAEFSQLMTAECKRGFRMYCENNLLVSDARKFKERYKALMKEALWAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQP7</accession>
    <name>H6QQP7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21228</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EHS62765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890182.1">
        <property type="nucleotide sequence ID" value="XM_003890133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQP7"/>
    <dbReference type="GeneID" id="13541333"/>
    <dbReference type="KEGG" id="pgr:PGTG_21228"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21228"/>
    <dbReference type="HOGENOM" id="CLU_491006_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQP7"/>
    <dbReference type="OrthoDB" id="2508725at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="549" mass="62846" checksum="D8F584319B38F75D" modified="2012-04-18" version="1">MRQLVSTTLSTKWMRGWSGAAISPCSSTELYYLPKFEEETPNIIGLAADNQDHLSILPPAVENFDVHSASFTPKIHEYWFNDHELDRFLESIRPNSPSLTHQQGGHQLLNRDHSNTNSPGLTHGEVGHDQLATRDYSNPNPPSSTDTEVGHRIQHRDSFPSDSPTTHHSALGNIAATSHEDAETTPSDPIQNRKRKLDRLVSDLYKSNPVQNEVKAKLLVQDEVKTKRLIRNELNTMRSRPSKWKLKLFGWVSTLKYAPGGQKDNESRFKYDLEGSEVWRKLGGEHGTAPALLNWLIGSGEVSKQQAVRRLKLLLKEISTRHGELLSVFLPMTYGRPSLKRKAHLIGTEEQTNLLNWFLRELEMKNHYSSNIRLEAPELHHDLSRHLSGIIRYLRIDPDVTRNSSSRWEARLPATRNISPQRVNKHQAETTRAALNTLETYYKLTNPHKWKQVFLEEDKFLNLFISLKNAHHHCNFGKSLKKSKEWATLAIFPWHVPLGLERSTGTKSALDSIHTTLKHVNVASINKHFVDLERVNNPFIQPTEKMRNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQP9</accession>
    <name>H6QQP9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21229</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EHS62767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890184.1">
        <property type="nucleotide sequence ID" value="XM_003890135.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQP9"/>
    <dbReference type="GeneID" id="13541342"/>
    <dbReference type="KEGG" id="pgr:PGTG_21229"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21229"/>
    <dbReference type="HOGENOM" id="CLU_2027881_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQP9"/>
    <dbReference type="OrthoDB" id="10586183at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="122" mass="13774" checksum="6649869D563A3309" modified="2012-04-18" version="1">MPFLFQLIYRKLAIQANSNTDTNQREDDSDDGEDPIPAAHVETDAIGDDLNDVLPFDCNQRAYMVAKTICSMVAFVKNRRNNGHQLANSLTFLACGVTNRPSDLYQDWETTCADFGIFSMFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>H6QR31</accession>
    <name>H6QR31_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dcp1p-Dcp2p decapping enzyme complex alpha subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21298</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EHS63006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890010.1">
        <property type="nucleotide sequence ID" value="XM_003889961.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QR31"/>
    <dbReference type="GeneID" id="13540936"/>
    <dbReference type="KEGG" id="pgr:PGTG_21298"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21298"/>
    <dbReference type="HOGENOM" id="CLU_045437_0_0_1"/>
    <dbReference type="InParanoid" id="H6QR31"/>
    <dbReference type="OrthoDB" id="2506989at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="476" mass="53345" checksum="F67D80B0B59915F5" modified="2012-04-18" version="1">MAKYIGAAKYKGVARKIRPVNQPMPQDLNPPLERPPLSRDPYKTPLTPFPPTFQETSRVTEKRLKARKIDCVLGRRAGNIKSLLRPALQDPVVWPSRHLPHYPSRKLHLDNSAPIRLLMVHTRRSTKDTLFGPLPPPTRRRRPISILDRRRKIEYRQLSKLPPLPPLPPLTPPEIDNAHSPQTEQLTTLIDNLNNFTISAVATTGSELPNRTSPIVPDIDNRPPSPFLSPISHIYSQFLTRQPSPTVQPIPMSTPTTSDITPRAKFLQQPSIYKSDVEQLAADGSNFDKWKRGLTRIILLTLGHANFFDKSENYSKLSTQENTCLLFLIQITINDELSSLVDQYTKGTEAYDAVQTNFQGTVRFRQMELIDKLLEFRVTGPSTEPSQIPGLFNKLFETFSGLTKVGAGLSPLVESLILQAVVPSPASMSRSQLFQNISLQLGKKPDVTARDIQTIITSAYGESLRFDSSPSTNVSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRI8</accession>
    <name>H6QRI8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21512</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EHS63296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889869.1">
        <property type="nucleotide sequence ID" value="XM_003889820.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRI8"/>
    <dbReference type="GeneID" id="13540697"/>
    <dbReference type="KEGG" id="pgr:PGTG_21512"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21512"/>
    <dbReference type="HOGENOM" id="CLU_109039_0_0_1"/>
    <dbReference type="InParanoid" id="H6QRI8"/>
    <dbReference type="OrthoDB" id="2514905at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="221" mass="24132" checksum="4C94B9166A80067C" modified="2012-04-18" version="1">MYSPSFRTPTTDVKSTPTPRQLAAVPPSDPPSSLTVVAIDQCRDPHQRPRRCRRYFHRIRRTSVPPSQMLLSSVVPSLGSSSARSMPRSPPTSSPACNHASIASAKLFVSTPSPTVCRAVYRCNLPTSPYLIEAKIPQCPRRLVTVPPPAPPSSTPLDSSSFSSDNVIALWSCHQLSSSDGEIRIRQTPFFSPPFVRQAQQHDASLFGRSSSFLEAPPLDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRT5</accession>
    <name>H6QRT5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_21482</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EHS63408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889777.1">
        <property type="nucleotide sequence ID" value="XM_003889728.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRT5"/>
    <dbReference type="GeneID" id="13543094"/>
    <dbReference type="KEGG" id="pgr:PGTG_21482"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21482"/>
    <dbReference type="HOGENOM" id="CLU_2027885_0_0_1"/>
    <dbReference type="InParanoid" id="H6QRT5"/>
    <dbReference type="OrthoDB" id="2511951at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003605593" description="Secreted protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS63408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="13453" checksum="D3A8EF2342CC41B4" modified="2012-04-18" version="1">MRATSTGWVMCMLGLGPALTGSMEIKMRCKACSAPNLTPTSTWAVKTCAEFRGEPPGEGNCGSSISARRWACPSCPVEEWRRQTNTPFCTQHPSTPPSFFVSPRPHPNARFGRLNRDHARED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSI4</accession>
    <name>H6QSI4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21797</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178294">
        <property type="protein sequence ID" value="EHS63721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889543.1">
        <property type="nucleotide sequence ID" value="XM_003889494.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSI4"/>
    <dbReference type="GeneID" id="13542789"/>
    <dbReference type="KEGG" id="pgr:PGTG_21797"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21797"/>
    <dbReference type="HOGENOM" id="CLU_2655667_0_0_1"/>
    <dbReference type="InParanoid" id="H6QSI4"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="76" mass="8099" checksum="0DC1E3BECC86FC30" modified="2012-04-18" version="1">MLPRAAVWSAGPSFSLILKDGSCIGASADRPRYVLVMPNSKAFATGEPPQHCSRGERKLAGLCDFEDKEAGSSMLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSR1</accession>
    <name>H6QSR1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21818</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178297">
        <property type="protein sequence ID" value="EHS63801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889458.1">
        <property type="nucleotide sequence ID" value="XM_003889409.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSR1"/>
    <dbReference type="GeneID" id="13541981"/>
    <dbReference type="KEGG" id="pgr:PGTG_21818"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21818"/>
    <dbReference type="HOGENOM" id="CLU_2004992_0_0_1"/>
    <dbReference type="InParanoid" id="H6QSR1"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="146" mass="15605" checksum="57DBF261C486F7F1" modified="2012-04-18" version="1">MFMPGGELSQVDLHPRTKLQIVLMGHIQGQPNLTTKQKARIAGMVEARMSHGRVARIVGSGQTKVSSTVSWARTHGTVKTTVNSGQPLWNSKQDLMQLQRSLASHPKSTLAEVTNTMATLVSSSTVQQRAHNIGNGGPATLTVALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTU0</accession>
    <name>H6QTU0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22183</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178322">
        <property type="protein sequence ID" value="EHS64359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889069.1">
        <property type="nucleotide sequence ID" value="XM_003889020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTU0"/>
    <dbReference type="GeneID" id="13541792"/>
    <dbReference type="KEGG" id="pgr:PGTG_22183"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22183"/>
    <dbReference type="HOGENOM" id="CLU_1291709_0_0_1"/>
    <dbReference type="InParanoid" id="H6QTU0"/>
    <dbReference type="OrthoDB" id="3142841at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052979">
        <property type="entry name" value="Adenylate-forming_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33927">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33927:SF1">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="172" mass="19258" checksum="A864EDDAD646BF76" modified="2012-04-18" version="1">MPSNKASIIRVPGGLTSGLHTRVSRGGLREWHIFGSISEGKHADCHYIVMAVQGGFTRAMNEDQPETLLFHRGVAICTGSGIGAVGSTCIQHDDWFLIWIGADLESTYGTEFMDFIKSKIQPERLLIWDTRGPLGRPDVNVELERVYKQWNAQSIYVFFSMDNHSSTKTHAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>H6QU33</accession>
    <name>H6QU33_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Mitochondrial inner membrane protease subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_22270</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178328">
        <property type="protein sequence ID" value="EHS64457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888984.1">
        <property type="nucleotide sequence ID" value="XM_003888935.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QU33"/>
    <dbReference type="STRING" id="418459.H6QU33"/>
    <dbReference type="GeneID" id="13541080"/>
    <dbReference type="KEGG" id="pgr:PGTG_22270"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22270"/>
    <dbReference type="eggNOG" id="KOG1568">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2868695_0_0_1"/>
    <dbReference type="InParanoid" id="H6QU33"/>
    <dbReference type="OrthoDB" id="308440at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042720">
        <property type="term" value="C:mitochondrial inner membrane peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.10.109.10">
        <property type="entry name" value="Umud Fragment, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037730">
        <property type="entry name" value="IMP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46041">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46041:SF2">
        <property type="entry name" value="MITOCHONDRIAL INNER MEMBRANE PROTEASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS64457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="64" mass="7201" checksum="2991ABD82C163343" modified="2012-04-18" version="1">MGHCWVEGDDPFHSKDSNSFGPIPLGLANAKVAWIVWPISRFGAPNSQSDDQSHRVRRMTVTRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUM6</accession>
    <name>H6QUM6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">hAT-like transposase RNase-H fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_22490</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178347">
        <property type="protein sequence ID" value="EHS64738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888775.1">
        <property type="nucleotide sequence ID" value="XM_003888726.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUM6"/>
    <dbReference type="GeneID" id="13541614"/>
    <dbReference type="KEGG" id="pgr:PGTG_22490"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22490"/>
    <dbReference type="HOGENOM" id="CLU_1540814_0_0_1"/>
    <dbReference type="InParanoid" id="H6QUM6"/>
    <dbReference type="OrthoDB" id="2505994at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025525">
        <property type="entry name" value="hAT-like_transposase_RNase-H"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14372">
        <property type="entry name" value="hAT-like_RNase-H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="hAT-like transposase RNase-H fold" evidence="2">
        <location>
            <begin position="6"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="83"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS64738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="189" mass="21753" checksum="B735BAD07A04DF16" modified="2012-04-18" version="1">MEGDGPTGSAAIPEYLQMKNTLKKKLDSSNRNDPLHPMFVKMFEKTNTYLLEALACETLVISTILNPSFRLAIFEKHFPKEALDAKKRLVELFEERKNQMAEKIQQEKEREKDTALKEKETNEDDIYSFFSGPSNNSGQDEIEMYLGGVDCIIHGNKKDYMFSALNWWKQQMSVLVLVASCCQEQLKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUN5</accession>
    <name>H6QUN5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22499</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178347">
        <property type="protein sequence ID" value="EHS64747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888784.1">
        <property type="nucleotide sequence ID" value="XM_003888735.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUN5"/>
    <dbReference type="GeneID" id="13541623"/>
    <dbReference type="KEGG" id="pgr:PGTG_22499"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22499"/>
    <dbReference type="HOGENOM" id="CLU_1816750_0_0_1"/>
    <dbReference type="InParanoid" id="H6QUN5"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="142" mass="15223" checksum="24934EF83F518074" modified="2012-04-18" version="1">MEAIGLMGVFKTARPFEGAPEMAKSAPLAGVTVPDQRIIPTQSDQARKALSMHTEEFRPTSTFPRTGPEPEAQDAKLPKNFNPQDLGTPSSVHTPALIPKIAAEDTKTRGGVGLSPVRVWEALVGPKGISDPLIRKTKNMPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQG7</accession>
    <name>E3JQG7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ER lumen protein retaining receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_00340</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ERD2 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307390.1">
        <property type="nucleotide sequence ID" value="XM_003307342.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQG7"/>
    <dbReference type="FunCoup" id="E3JQG7">
        <property type="interactions" value="191"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JQG7"/>
    <dbReference type="GeneID" id="10546814"/>
    <dbReference type="KEGG" id="pgr:PGTG_00340"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00340"/>
    <dbReference type="eggNOG" id="KOG3106">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057784_0_0_1"/>
    <dbReference type="InParanoid" id="E3JQG7"/>
    <dbReference type="OMA" id="WKSRSCE"/>
    <dbReference type="OrthoDB" id="7694678at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046923">
        <property type="term" value="F:ER retention sequence binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045015">
        <property type="term" value="F:HDEL sequence binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006621">
        <property type="term" value="P:protein retention in ER lumen"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000133">
        <property type="entry name" value="ER_ret_rcpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10585">
        <property type="entry name" value="ER LUMEN PROTEIN RETAINING RECEPTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00810">
        <property type="entry name" value="ER_lumen_recept"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00660">
        <property type="entry name" value="ERLUMENR"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00951">
        <property type="entry name" value="ER_LUMEN_RECEPTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10 12" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="66"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="151"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP74384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="213" mass="24639" checksum="FF63258C4A335651" modified="2011-01-11" version="1">MNIFRLIGDLSHLASIFILIQKIIKSRSARGISFKTQVLYVVVFLTRYVDLVTGPFISIYNTAMKLFFIASSAYIVYLMHFKYKPTQDPAIDTFKVEYLLGPCALLALVFNYKFTVVEVLWAFSIYLEAVAVFPQLFMLHRTGEAETITTHYLFALGLYRAMYIPNWILRYTTENTLDPIAIFAGIVQTGLYADFFYIYFTRVMRGQKFELPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRY2</accession>
    <name>E3JRY2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_01400</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
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    </dbReference>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01400"/>
    <dbReference type="HOGENOM" id="CLU_1816738_0_0_1"/>
    <dbReference type="InParanoid" id="E3JRY2"/>
    <dbReference type="OrthoDB" id="2508489at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP74807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="142" mass="15367" checksum="884BD160406CEEB2" modified="2011-01-11" version="1">MVIRPACQRNWSHRGDEQIVTLDDHVRKYLVGQSNLGSLRKSAAGSCQAYVKVTLQLGQSCIEYVAGSGLRKAPRHSQTEGQGLSTRVDTCLFLCEPPGGAWRKAGRTKPAELLLGAPIRPAGHCQQYGALRLLPAVTCPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>E3JS45</accession>
    <name>E3JS45_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_01509</name>
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    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003319287.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JS45"/>
    <dbReference type="GeneID" id="10547118"/>
    <dbReference type="KEGG" id="pgr:PGTG_01509"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01509"/>
    <dbReference type="HOGENOM" id="CLU_3207849_0_0_1"/>
    <dbReference type="InParanoid" id="E3JS45"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP74916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="45" mass="4971" checksum="540B99A6B6085C8D" modified="2011-01-11" version="1">MGPLNRLALNAACVAILPPQKLVNSTNFCERLLQRRYATLFGPKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUB2</accession>
    <name>E3JUB2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chitin-binding type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00968</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75637.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320056.2">
        <property type="nucleotide sequence ID" value="XM_003320008.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JUB2"/>
    <dbReference type="GeneID" id="10543437"/>
    <dbReference type="KEGG" id="pgr:PGTG_00968"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00968"/>
    <dbReference type="HOGENOM" id="CLU_2085952_0_0_1"/>
    <dbReference type="InParanoid" id="E3JUB2"/>
    <dbReference type="OrthoDB" id="2508050at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171542" description="Chitin-binding type-2 domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75637.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="12795" checksum="C1CE90313F020E47" modified="2012-04-18" version="2">MPTIKSIFLFTFACLLSAPMVKGSPRMDPASSGCAHSIRSTASKDFTCQATYICYAGYSHPCGYGLTGEVEQCESCNHYSILSISPCPYGNHPSRSCRKHAVVNPRAGERRKYTDMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3JYG6</accession>
    <name>E3JYG6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_03047</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321510.1">
        <property type="nucleotide sequence ID" value="XM_003321462.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JYG6"/>
    <dbReference type="GeneID" id="10529439"/>
    <dbReference type="KEGG" id="pgr:PGTG_03047"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03047"/>
    <dbReference type="HOGENOM" id="CLU_305254_0_0_1"/>
    <dbReference type="InParanoid" id="E3JYG6"/>
    <dbReference type="OMA" id="DICNINT"/>
    <dbReference type="OrthoDB" id="2503462at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="138"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="181"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="477"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="593"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="676"/>
            <end position="748"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="791"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="8"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="109"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="187"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="209"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="247"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="285"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="340"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="380"/>
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        </location>
    </feature>
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        <location>
            <begin position="396"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="427"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
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            <begin position="497"/>
            <end position="507"/>
        </location>
    </feature>
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        <location>
            <begin position="516"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="558"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="602"/>
            <end position="614"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="677"/>
            <end position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP77091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1098" mass="121003" checksum="B61F52B68B6C631C" modified="2011-01-11" version="1">MASRPPHSNRFNYNNNNLNPTASTSSSTLVNELQDEKEKQQRLEYQATAGIHIFEAEGALPTHPLQPQTHLHHPQRKPFQPERDQKLFKTLINSPSKLSRTTAKRRSIGSLNQTTNNLQNHSETAPIMASLKQNQISLSSDQDNNHAPPEDREDQDPDATFNFDPNDGTKRMAALFDSVAHSDMPPNVNQTTKNNISPHLDSSKPVQHHQTEPKPKPRPPLEHLRGRFDKQNQETRRIPSDPPIDNVNRTNGLSTNVTSRSHASSKALSTQRVQAHNQLGHHPTKTSSSQNIAKQPQEPLLSQNTISKDGWRSGPLMTTRESTSDTDDSPEDTFKPPVSSDLNNPSRHSVHNNLPAQKSLGRGPSDAQKKVTFSRRLNDSNDLLTSGSSMDPADLDPARSDSTDPEDAQRQTEPNTSYVNPEEEGQPEQTLQNPRLEAGQTTLTTPKIAGFYPSTPATIRPTAVASRLLANEEASRRLQKLKNQTPRARLKRLPSALQDQPVQTWDAPNQIDYSAMTGNHLQPSTSQASKHTFQAPPLTRNGKGKEKQVEDPPPSSTEPPQTHPTGQVNRIVQESYDQTIITGGQTRASCFPTPHPPGWLVPGSPGEPSSSTGPADRPILHPTSALKHARQKASSRYGSAHDRVIYASNHAVDEPADEITPRPSKRPAHRTLVAQKSALNKDTQQISTSNPNFTLPSFDGFDHPRQAHLTSTPVVPPLPPHHSKNPNPGPPSTTTTQTTDGGGNGPGKTLKDLAAAVIEFIVDKKPIEVLADTFSMNSNHANNKVEKKKTIQAKKAELLTQEKSAKELEELMRSSERVEEERRRIQAKLDDLCPPHSLHSRPSRLLSVLKTFFFSNSGQQASPTRTTVNRREPRVADRHVPCWIRRHWKVSLLALLFQAACLWMVLHLSTIRSYNLVLQSTLSSTPTSVVVGGAGHPSTTSTTSTSMIVSGSLSYMSRDRLARVLRSQTGAHRLDDPEHGGIYEGVVELARFLADWAAGFPAFLHPFHHPHPHSSSSYSLLPSLLHPLRSSPLFRFFFPPSSASSSSSFISPVATPFNLSSQAPPGQQDLPLQFATAPDSLDPIQSLLNYQFVPFVPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3JYJ4</accession>
    <name>E3JYJ4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77119.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321538.2">
        <property type="nucleotide sequence ID" value="XM_003321490.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JYJ4"/>
    <dbReference type="STRING" id="418459.E3JYJ4"/>
    <dbReference type="GeneID" id="10537330"/>
    <dbReference type="KEGG" id="pgr:PGTG_03075"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03075"/>
    <dbReference type="HOGENOM" id="CLU_1090452_0_0_1"/>
    <dbReference type="InParanoid" id="E3JYJ4"/>
    <dbReference type="OrthoDB" id="10311898at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="243"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77119.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="255" mass="27972" checksum="C9BC6E562298D4A3" modified="2012-04-18" version="2">MRVIQQANVDNFDALLANPSAITKDPNLLKMTRKRNKTTPKGTLSYPSAVAQDLVHHLVHSFEVFYGELLGPQAKFPAAAFFGVEQATIIVGSMDQICSRGSQNMGLMGVQCFPGQLESLNNAIIQWMASKVYLSHLLQTANLTRFIKSEGLQVQEAMAAKPAALQSQAKARRQAKKNCQSSGQGPRGFKTKQDTYHLCVSGRPSNILAGLRTYRNSLDGYRQYPTAPPLCHLPPGGSLRNHQKLDAATKRALHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZ39</accession>
    <name>E3JZ39_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_03270</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77314.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321733.2">
        <property type="nucleotide sequence ID" value="XM_003321685.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZ39"/>
    <dbReference type="STRING" id="418459.E3JZ39"/>
    <dbReference type="GeneID" id="10537860"/>
    <dbReference type="KEGG" id="pgr:PGTG_03270"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03270"/>
    <dbReference type="HOGENOM" id="CLU_1220211_0_0_1"/>
    <dbReference type="InParanoid" id="E3JZ39"/>
    <dbReference type="OrthoDB" id="10268103at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.170">
        <property type="entry name" value="Formyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP77314.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="227" mass="25112" checksum="2D74FE3924D11F0F" modified="2012-04-18" version="2">MIGHILIPHCAVAIVRLACQMRVLSCGSDSFSPTILNRLIHGNRTLEMFSLIPASSQTRGGLKKVEDLNIPHHWPRTENRQRPLKHWSIPDEFTQPEDDELVPILLSALFPYRIPREMIEDNREIQASTGAERSSLTTMVPGCGADPMSVSRQGARAGVSMQEVSPLGFETSKILAQKPILLSPTICYPAAENALANLVADMLHQLIPNIDSYIDTATYLPLKAPLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZM8</accession>
    <name>E3JZM8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03459</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321922.1">
        <property type="nucleotide sequence ID" value="XM_003321874.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZM8"/>
    <dbReference type="GeneID" id="10538541"/>
    <dbReference type="KEGG" id="pgr:PGTG_03459"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03459"/>
    <dbReference type="HOGENOM" id="CLU_756805_0_0_1"/>
    <dbReference type="InParanoid" id="E3JZM8"/>
    <dbReference type="OrthoDB" id="2499550at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="195"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="366" mass="40720" checksum="E5EA7036FB5056E8" modified="2011-01-11" version="1">MSLNSSIGLSRKPSQRLLSAVRSIKQKVNRPKGQPKDANSLGTRPLSETILDINFQGGSSTSTTANCRPTCWPMLAPMMFEFEILPRFYRAAPYSSSHQETDTARSVPYGQNVQSTNVNHRNAQEKRNVGVFRVCQVSPTLSRCLPSTPPLGFGKLGDQNKPEQPLPRLFCPPPRCDRTDSLVQPPCSPYRSSEDSSSSQESQESDYASRVSIRLSVEIVQSFFDHTGPSSPCSESSDEELYAEKISARLENLCYDKPLEFIHRRSHAGKGNANKRISSFGVARIIISSPNALQKPLLSPSFKSWLDKFIESRLNQDESLNGFHGSFESMIPEETHSAIQEEVCEEMNPVPVLKANHDTRNSLDAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3K0W9</accession>
    <name>E3K0W9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Laccase, multicopper oxidase, benzenediol:oxygen oxidorectuctase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_03900</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the multicopper oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP77944.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322363.2">
        <property type="nucleotide sequence ID" value="XM_003322315.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K0W9"/>
    <dbReference type="STRING" id="418459.E3K0W9"/>
    <dbReference type="GeneID" id="10541092"/>
    <dbReference type="KEGG" id="pgr:PGTG_03900"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03900"/>
    <dbReference type="eggNOG" id="KOG1263">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_006504_7_1_1"/>
    <dbReference type="InParanoid" id="E3K0W9"/>
    <dbReference type="OrthoDB" id="2121828at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13904">
        <property type="entry name" value="CuRO_3_Diphenol_Ox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001117">
        <property type="entry name" value="Cu-oxidase_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011706">
        <property type="entry name" value="Cu-oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045087">
        <property type="entry name" value="Cu-oxidase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709:SF414">
        <property type="entry name" value="ADR239WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11709">
        <property type="entry name" value="MULTI-COPPER OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00394">
        <property type="entry name" value="Cu-oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07731">
        <property type="entry name" value="Cu-oxidase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Plastocyanin-like" evidence="2">
        <location>
            <begin position="194"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="Plastocyanin-like" evidence="3">
        <location>
            <begin position="477"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP77944.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="602" mass="66240" checksum="22E17569EF1F7CF7" modified="2012-04-18" version="2">MGFYDDRSRGPHIGTIITWILSFHLTYTISSSLSGSNFLPNSNLRTSSKFPKNTTSLPVRTAFAGSVKVNSPTLVLNAAHVVTSVPQIRRFDWAVAKKTAAFDGFKRSVFVINNQFPGPLIEANEAFTKGVPNGVVDCFVNLFDILFQFISMDDSLKLAKAHYGALLADGISGPLIIHSPRDPLVRGKDFDNDQILFLNDWYHDPSTTITRQLLSFKGYNGSLAAPSPNTALLNGIGFFNCEKYGDGKPCQTNNSPLEIQVPPRQRSRIRLIQAGSHALFKVSIDNHPLEIIEADATPVSSSTLFHRINLHNAERYSVILDTREDREGDSFYLRATMDTDCFAWLAPGMDTAEGNTALAIIRVSSKPLKSGRARSVAARPTTLDWKDTLNGPCIDLDQTKLSPRINPKLKTNSLGRVYFNTSFGTIIDKSLKVPNNTLGRFFVDNTTWISRDSKPLLPELLRGGSKSLDSSEVATQTITRPGIYDVVVNNLDQAIDHPIHLHGVDSCVVASGNGTLSESNLQLAHYELDNPLCRDVHVVPGGTFLVMRIRADNPGIWIIHCHIDWHLAAGFAGMLVIQPDVLEQTVLPQENQGLCLKGGKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3K1R9</accession>
    <name>E3K1R9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_04200</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP78244.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322663.2">
        <property type="nucleotide sequence ID" value="XM_003322615.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K1R9"/>
    <dbReference type="STRING" id="418459.E3K1R9"/>
    <dbReference type="GeneID" id="10542036"/>
    <dbReference type="KEGG" id="pgr:PGTG_04200"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04200"/>
    <dbReference type="eggNOG" id="ENOG502SXDT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_664171_0_0_1"/>
    <dbReference type="InParanoid" id="E3K1R9"/>
    <dbReference type="OrthoDB" id="2503018at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="207"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="327"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="135"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="328"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="363"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP78244.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="387" mass="41894" checksum="8BC78397F8A80AE3" modified="2012-04-18" version="2">MTTRSLSQPQLSSHSAAQKLILTKEPNALATSTTKFGPSTFHSRRQTARNLSNRHRRIARRGIFPDISNFASNTFGGFFGNSNNGLAPQSGPATQNSRQVTYPQDSQPFPSGDSRALMNGQPNAPLPHPTQPPNSAGGSSKSPSPSTTTTTSLKSQATAVSHHSPSSATEASTTSAASAAPTDEPAPRSALERFAALYKPTNRFEPLAVLLTIVFILAFLVTLMSILKCVAHKKRFANKKDYPTGFPWVDPAGSKTNRTSSRQGLVATKSYMSNRNSMTSSVYFSSGRQLGPEGELTPHQAEYQLNELGSDVYANYAWAATEMDLEKNLSSPSDEGPSSATTEEGNHTPNQQHDRWHTQSLDDDPRFTRGGKGELLEDSKVFKLDHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K231</accession>
    <name>E3K231_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_04356</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78400.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322819.2">
        <property type="nucleotide sequence ID" value="XM_003322771.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K231"/>
    <dbReference type="GeneID" id="10535998"/>
    <dbReference type="KEGG" id="pgr:PGTG_04356"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04356"/>
    <dbReference type="HOGENOM" id="CLU_1396951_0_0_1"/>
    <dbReference type="InParanoid" id="E3K231"/>
    <dbReference type="OrthoDB" id="10622335at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172314" evidence="2">
        <location>
            <begin position="21"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP78400.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="195" mass="21743" checksum="37BC4872770AE9BE" modified="2012-04-18" version="2">MKRGKYLVLLGLWGIAVTSTNFDFVSTHAGPLLEAYTPHFVVQNEDVNFNELHEYLTRLEGTNLSSSSLRGQAEASTTDHTPAQMLNESRRAYTKHLMESPGACPPAMSKSGDCGQSLNMRFDDGSKSEDSVTNKSSKILLATSTTDEYTPEIFKEMSACQVMDELIDYNLDDYFDFMESGARFQSIFKADNGQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>E3K375</accession>
    <name>E3K375_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_04888</name>
    </gene>
    <organism evidence="7 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <reference evidence="7" key="3">
        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC10 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78932.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EHS62560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323351.2">
        <property type="nucleotide sequence ID" value="XM_003323303.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890273.1">
        <property type="nucleotide sequence ID" value="XM_003890224.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K375"/>
    <dbReference type="FunCoup" id="E3K375">
        <property type="interactions" value="506"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K375"/>
    <dbReference type="GeneID" id="10537120"/>
    <dbReference type="KEGG" id="pgr:PGTG_04888"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04888"/>
    <dbReference type="eggNOG" id="KOG3745">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008002_1_0_1"/>
    <dbReference type="InParanoid" id="E3K375"/>
    <dbReference type="OrthoDB" id="125856at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009976">
        <property type="entry name" value="Sec10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048627">
        <property type="entry name" value="Sec10_HB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048625">
        <property type="entry name" value="Sec10_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12100:SF0">
        <property type="entry name" value="EXOCYST COMPLEX COMPONENT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12100">
        <property type="entry name" value="SEC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07393">
        <property type="entry name" value="Sec10_HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20667">
        <property type="entry name" value="Sec10_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0268">Exocytosis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Exocyst complex component Sec10 N-terminal" evidence="6">
        <location>
            <begin position="50"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="domain" description="Exocyst complex component Sec10-like alpha-helical bundle" evidence="5">
        <location>
            <begin position="173"/>
            <end position="826"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF20667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP78932.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS62560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="863" mass="95879" checksum="065EE7534230D32B" modified="2012-04-18" version="2">MADDPADQQSSSTLSLSTFEEPTFSVASLLAGLTEGLIKEDKQSGKAFNPDPFIATLEGAIEQLLPLQDQVDKKIKTLEKDVAQKERSYRTRIDDFKNGLNNVTQEFSGLNSKITEVGKTAIRIGEQLESIDRVRSRATEAHDIILYYNEFARGETTRLEALRKEGKDGRAKVAVIARRLLTVAREIEGVDGSEVTKATIEKYAERFEKDMLRLFEKAYLKGEPKAMAHCAQTLLEFNGGSSCIQIYVNQHDFFISRDRIEAVDYVAEAPMWESLADPNQAAPKTEPQLAALYSDIRDQISQEAQIIEVVFPHPVVVMQVFLQRVFAQVIQSHLEKLINAAQGSSTLAFLRVLALARFSTAQLVNDIKAHDFFRSSSSISSSTTETYTLARRGSDDADVDNKASVGVIQAISGGPGISALSSMLDQQLEELFGQHLDNSRYVERECKSLTELYASYLLQFARWHRATNKAKPTNTIFDRMVNQISSSANGTPGTTQTSGLKSLLKLSGISADDSANPLNEELPTTPELCESDGQLDLNVAEKLLTWHAESVGRMIELSSPSEVPKNVFSLLKTLAESFCKGYIETALETSLAQFSSYDFKAEPSLKPMTVIKTADMAMHLWQRYVTTAIVPLAAVSVNIRREMSIFNNHILVRIENKINSVAQKALECVLSWLSISLAKQKKLDFKPKNDELDFSRTHTEACAACTGFLNTTRTIVNQSLSGKNSEIFLSEVGVVFHSFLLEHLKKFPVSAMGGLMLTKDLTMYQDTIAKFNIPGLNERYEMIRELGNIFVVQPSILKSYLGESAMLGRIDGKLLRPFFLMRVDYGDHPKKFWDEVVGESKHHELGAAGAQVDRGLWLGITGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3H4</accession>
    <name>E3K3H4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_04987</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP79031.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323450.2">
        <property type="nucleotide sequence ID" value="XM_003323402.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3H4"/>
    <dbReference type="GeneID" id="10530852"/>
    <dbReference type="KEGG" id="pgr:PGTG_04987"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04987"/>
    <dbReference type="HOGENOM" id="CLU_067863_3_1_1"/>
    <dbReference type="InParanoid" id="E3K3H4"/>
    <dbReference type="OrthoDB" id="2495471at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021851">
        <property type="entry name" value="DUF3455"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35567:SF1">
        <property type="entry name" value="CONSERVED FUNGAL PROTEIN (AFU_ORTHOLOGUE AFUA_1G14230)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35567">
        <property type="entry name" value="MALATE DEHYDROGENASE (AFU_ORTHOLOGUE AFUA_2G13800)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11937">
        <property type="entry name" value="DUF3455"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172372" evidence="2">
        <location>
            <begin position="23"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP79031.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="243" mass="26211" checksum="BD06D879B983F90F" modified="2012-04-18" version="2">MLFTLNLRLLGFLTLLVAGAWCAFSVNDFPAGLPRPRGTPKYLALAFGTQHYTCQGGIWTPHGAQATLTAESLPRSPKSPRSTIPESDEKNTHRKKTGGKRTGGKRTGEKRTGGKRTGGKKTGGNNTGGTSNGERNNGGKNTGGRSDGKHRSTVGHHYFELPQQPTFQINGLGTIVAEKKKFVPSPYPEKHGVPWLKLDVVQGTFARTVYRTSTVGGSPPSTRCKATDQPLKVPYSAIYSFWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3K428</accession>
    <name>E3K428_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04786</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323249.1">
        <property type="nucleotide sequence ID" value="XM_003323201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K428"/>
    <dbReference type="GeneID" id="10536851"/>
    <dbReference type="KEGG" id="pgr:PGTG_04786"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04786"/>
    <dbReference type="HOGENOM" id="CLU_874745_0_0_1"/>
    <dbReference type="InParanoid" id="E3K428"/>
    <dbReference type="OrthoDB" id="10308694at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="318" mass="35018" checksum="EE93FC8779E0EF5B" modified="2011-01-11" version="1">MLHSDPVFIGKCRRFRQEQCTTQAAPFSQLGLRMCCTIEHSPYGPIDPIALFASLSNDPPSLNYLFHSTPTPQVQPSNLPNVATSPVTSRPENVENQPSRSRLGEYLDAIPGSINTAPPGHPVPPNGLTDFRTDFDRHTRGTHVEAHQAPVSNNRQMKEVAHTELPRIIPYQQAPSIDSHLHDPSAKRPKFNRPSPGALHRPGRVLVETSSDYLPIQSGVNIPLEENLGLRSDADTLGGDTFGGASTSHTRVNEYQNGNLPEMSMVPDSGTMPQMAQKAEIESTFGRTKSEFSGLIEKPFIVNMYHRNISPTQTQSSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K474</accession>
    <name>E3K474_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05405</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79084.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323503.2">
        <property type="nucleotide sequence ID" value="XM_003323455.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K474"/>
    <dbReference type="GeneID" id="10530914"/>
    <dbReference type="KEGG" id="pgr:PGTG_05405"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05405"/>
    <dbReference type="HOGENOM" id="CLU_018307_2_0_1"/>
    <dbReference type="InParanoid" id="E3K474"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="319"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79084.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="425" mass="46620" checksum="C5D5ECC22F996770" modified="2012-04-18" version="2">MSDKTAPAHETSGSTRTADASDMQTAKDWFKSVFKLQHAAIIEAHNDRRQAAEDANAARISRLEELILAMNVKNEADARPVQPSPGRVDLQKFRTSDGPIYRRPFQVVEPFLRWIHGVQIFFDTKDVSNSANRIKILGNLIAETNLQSFCANEASGFLTKSWNDFKIRMFDFALPTNWCLGLQQQTSKLGDLQLAQFLVYGLPDALQDRINERQLLEVVPFAYGTFEKQASASFLALHRPTVPPAPTRSTPSAPSNLARDEFVWRVHLYLDSLGLCHFCKKHCGNAAGACPGPIDRSRIDIPSGFATPEKPANYVAPRAWSRPTATPGKPTQPPAGRPSTRAASVAGVTDTTPLEAQVSVLTLDAAIREDNRWDTYFDDEGCFPSLEPAAVAALEDLNSQLLANEIAKAEQADLADAIAGHPGRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3K5K4</accession>
    <name>E3K5K4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_05782</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323880.1">
        <property type="nucleotide sequence ID" value="XM_003323832.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K5K4"/>
    <dbReference type="GeneID" id="10531289"/>
    <dbReference type="KEGG" id="pgr:PGTG_05782"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05782"/>
    <dbReference type="HOGENOM" id="CLU_694708_0_0_1"/>
    <dbReference type="InParanoid" id="E3K5K4"/>
    <dbReference type="OrthoDB" id="2508739at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173248" evidence="2">
        <location>
            <begin position="29"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP79461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="401" mass="46795" checksum="202564338C1E373E" modified="2011-01-11" version="1">MRSDCRGVFALFPLLFFSLLAAPPPLRAVPADITYETSAFEPHWTPQPSLLQKIPRRPDASPSQESHEVVDIGTVILRKANLVLDRLISAGERTRGLKVDKHITWGNINYEPRLTEQREQIARRTFRFRAVYRLASKSEKDRMDEILGKQPDEIVQTISRIDRLCDIVFHGDAKNPAVEQKILQETQWFQERFDGIFDAVHSEQIEEMILKETNSKRMIPSITIQNFHEKLAAIKELNQKTSILKLHRKLDQAIGRYYNSVRYDQPIKQWDQDKQSIEEWDQKALISSLYPKLDALFRQLAIERGYYLGQYHPPSAKWDQETLILKLPATLEDVYRGQHSDLQYFPVGYDPSIKNWDQEALIANFHIKLDEALSKLRINEEPDSAQKSDWKRIFDCLAWLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3K789</accession>
    <name>E3K789_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05224</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EFP79999.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324418.2">
        <property type="nucleotide sequence ID" value="XM_003324370.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K789"/>
    <dbReference type="GeneID" id="10533853"/>
    <dbReference type="KEGG" id="pgr:PGTG_05224"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05224"/>
    <dbReference type="HOGENOM" id="CLU_1050257_0_0_1"/>
    <dbReference type="InParanoid" id="E3K789"/>
    <dbReference type="OrthoDB" id="2510020at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79999.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="265" mass="30580" checksum="DCA2ED09716719E8" modified="2012-04-18" version="2">MSLFKGKDSVPEYERLVDESHELAEPSNEKASHQIRLLTSITTHTSSCGPLFALLVSSSFPGFLRFSKNKNKKTASSSQSEPPEERDARSTAIVEDPIDSEEAITSHEKALAEKIENITNYVDKLYKKKYAFSWLQGTNLDEDLFKAFDARYIYGEPTLRALKKHFEAEMRYEQVPNKLSPKMESLIKNALQELSKLTKKDDQTGIKIAYYARVFAYMSGYHRNSRTPFNCLIMSHIKKNIQTSVKCLEKKYRPIFNQLNRIFER</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>E3K7M4</accession>
    <name>E3K7M4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="3">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_06278</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="3"/>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80322.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324741.2">
        <property type="nucleotide sequence ID" value="XM_003324693.2"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K7M4"/>
    <dbReference type="GeneID" id="10539330"/>
    <dbReference type="KEGG" id="pgr:PGTG_06278"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06278"/>
    <dbReference type="eggNOG" id="KOG1864">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_262739_0_0_1"/>
    <dbReference type="InParanoid" id="E3K7M4"/>
    <dbReference type="OrthoDB" id="27652at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02663">
        <property type="entry name" value="Peptidase_C19G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000161">
        <property type="entry name" value="Related to deubiquitinating enzyme ubh1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF733">
        <property type="entry name" value="RE52890P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="USP" evidence="7">
        <location>
            <begin position="39"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="87"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="347"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="591"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="643"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1005"/>
            <end position="1044"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1077"/>
            <end position="1285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="10"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="87"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="131"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="147"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="178"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="208"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="256"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="363"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="591"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="643"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="659"/>
            <end position="681"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="682"/>
            <end position="743"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="748"/>
            <end position="762"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="778"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="835"/>
            <end position="857"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="858"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="951"/>
            <end position="988"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1005"/>
            <end position="1030"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1090"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1138"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1214"/>
            <end position="1233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1239"/>
            <end position="1248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP80322.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1285" mass="139751" checksum="32FD600DC0E478CA" modified="2012-04-18" version="2">MSLLRWMGVNSSGSQSNQNLNSNEAQNDQWWTGSDEKYFGMENFGNTCYANSVLQALYFCKPFRQLLELSSADSHFLLQANQQTAINQSTSSSPQSPNQQHTHILKTPTSSGLSSSSDNLKTHSRNHTRSKSTSLGLDTLQKNHLNQQQQPTSSSSTSPTTRPRSKSNNQLHLSKDLSSSSSSSAASSSSNSKPDQQLQHPNIHLKTDSNTLRLTQNQPSAHSKTPIPTSTSTNASHTKRNWGAADFDQGSLGRIQHTNHNQQNPTSSHLEPETDQDPTICSSLRDLFRHISTQPNSVGAVAPQAFITTLKRYNELFRSTMHQDAHEFLNYLVNSVAEDVFAEQEKKRLEDERSSLLEPTPPSLQNLNNTSTPSSKKGHPRSTWVHRLFEGTLTNETKCLTCETVTQRDESFLDLSINIHQNTSLTACLRQFSASEMLCQKNKFSCDQCCSLQEAEKRMKIKKLPNVLALHLKRFKYQENLQRYTKLTYRVVFPFELKLFNTTDDIQDPDRLYELWAIVVHIGAGPHHGHYVTILKSHGQWLLFDDNIVTRIEERDIQKYYGDTPGVGSGYVLFYQATDLDLMDLMGVPAEPEEAEEDSEVGTDDSDDPIPAIDDSTQGSLFTARSSPLSAEIKKELPQLSSWNGFQSSSVDNPVRKNSAPFSSGLSSSSSSNSHPLPSTSQLQATAGSSSISNRTLSTNDRSELPTSNHSNKPEFSTPPSSQMEDNNPQTSAKVQSPQTKISGTGVLSRSPSTSTKYSSLPRSGPHLAHNPIDVRFSPQSSPESGLLSPFQTGPSLPKNVLPNAPVRTIPNLPSPKTNDESSSRSAMAAFLPTSPLTAPSSNGNSSKPTSASTTPNLHRKFSAKFLRRKKSSASSSSPVQPPPKTADPSPRSSPTVGGNESVGGLGSRSSEQALRNPSVHHYHQPILSSPLGPDSTDKHPHHQQQHFFSPRTTNLGSYRHSLSTALDHSIPGTSSNAPTSLPPSNRNSIHSVFHSISHSSAFIGPSPHLSSSPIPSASGTTTTTTTTTTKGAQAPPATSILTEKQQREIKKASEKANKLAAKHKLKALKDRSNSKFISTTTPFDKDHSTTNSSSSNRLAAQFKFSRTLSPVESHTNNRSHPPLEPPSLSPHYKGIISPTNVTNQERQIITSPSSSASRKSFGSVVGTQAPPPSTASSHYLHHSLPKLGRPHSASLRSSLTHTPNPNPNPNPVSLPHSLPTSNPNQNSNLNSNVKANRISASLANRPSSAKRPSIIGPALAPTHKPAAGSLSDFFMGRKSSSASS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3K7Q0</accession>
    <name>E3K7Q0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_06304</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324767.1">
        <property type="nucleotide sequence ID" value="XM_003324719.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K7Q0"/>
    <dbReference type="GeneID" id="10539356"/>
    <dbReference type="KEGG" id="pgr:PGTG_06304"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06304"/>
    <dbReference type="HOGENOM" id="CLU_1571396_0_0_1"/>
    <dbReference type="InParanoid" id="E3K7Q0"/>
    <dbReference type="OrthoDB" id="10512612at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP80348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="170" mass="18387" checksum="1DB5DFFEBEF1702A" modified="2011-01-11" version="1">MTQSARKSLAGKYLTTIDAAREQLSGLGLELLIDKDSLFPRRSSHFQSLPQALLLSPDSTLPIRDFPSLPNGPRGNIFVTYTRKHVQSLELRSARFATQGSICLCVATTQISLRSMVLPQGQHRVLKGCLAGDADQLSLDSVHLGKGGAGFNIAHTPLSDATESEELESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3K7T1</accession>
    <name>E3K7T1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_06136</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324599.1">
        <property type="nucleotide sequence ID" value="XM_003324551.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K7T1"/>
    <dbReference type="GeneID" id="10534530"/>
    <dbReference type="KEGG" id="pgr:PGTG_06136"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06136"/>
    <dbReference type="HOGENOM" id="CLU_033090_3_1_1"/>
    <dbReference type="InParanoid" id="E3K7T1"/>
    <dbReference type="OrthoDB" id="10279179at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP80180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="375" mass="42531" checksum="F83D887C20FCB2AA" modified="2011-01-11" version="1">MKEFGRLGEYNFCWRTGAQTEETPIEQLRSKNTLRSRLDSDLLPSINEQCSRLAGLPRDPPHVGQDPALYLKIIAEIQANLHQTLPQTIQTINALFPNPMPKPDDETNDQGFNEFKIYRLYHLEASIRTDLQVGLLSLFSEFHQFLRNFKDGGDTNPCLIEFAVRDINQIIDWAIKLSRASELSVIWTLWKNEIPGCDEELEGMLIQMGPTRLLDGEHEALLSPPAVELGTSLIPVFKLSRLFFNKLSQERVTKEDVGSFTEMSSAQLSTLDGWIEQIFSSIASMHFSIKNADEDDPVNTSAAIIDELETLKAVFRSYQLLIDKYILPNLFPNSLDLSSRVCFKDWLDSWTTSFSKAIHNAIHAANTYESSTIIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3K8G1</accession>
    <name>E3K8G1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BolA-like protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_06557</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the BolA/IbaG family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80601.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325020.2">
        <property type="nucleotide sequence ID" value="XM_003324972.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K8G1"/>
    <dbReference type="STRING" id="418459.E3K8G1"/>
    <dbReference type="GeneID" id="10539614"/>
    <dbReference type="KEGG" id="pgr:PGTG_06557"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06557"/>
    <dbReference type="eggNOG" id="KOG3348">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109462_0_0_1"/>
    <dbReference type="InParanoid" id="E3K8G1"/>
    <dbReference type="OrthoDB" id="203381at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.90">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052275">
        <property type="entry name" value="Mt_Fe-S_assembly_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46188">
        <property type="entry name" value="BOLA-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46188:SF1">
        <property type="entry name" value="BOLA-LIKE PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="35"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="43"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP80601.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="158" mass="17015" checksum="C45BCFCD9F7220C9" modified="2012-04-18" version="2">MIRQSAFTRSLFSKPRAVLPSRVFVRETVAARPGHQRPFNTTLSSLSISSSSSANPPLDSSASSADQTPVSEASISAKLARKLPQAVKLRVEDVSGGCGSFFVVEVVDQTFEGLSMLKQHQLINRTLADDIDRIHGLQVRLLDPSSSTLAILPISLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>E3K8T9</accession>
    <name>E3K8T9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_06601</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">The C-terminal domain binds 2 Fe-S clusters but is otherwise mostly in an intrinsically disordered conformation.</text>
    </comment>
    <comment type="domain">
        <text evidence="9">The N-terminal domain has structural similarity with S-adenosyl-L-methionine-dependent methyltransferases, but does not bind S-adenosyl-L-methionine. It is required for correct assembly of the 2 Fe-S clusters.</text>
    </comment>
    <comment type="domain">
        <text evidence="9">The twin Cx2C motifs are involved in the recognition by the mitochondrial MIA40-ERV1 disulfide relay system. The formation of 2 disulfide bonds in the Cx2C motifs through dithiol/disulfide exchange reactions effectively traps the protein in the mitochondrial intermembrane space.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the anamorsin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80645.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325064.2">
        <property type="nucleotide sequence ID" value="XM_003325016.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K8T9"/>
    <dbReference type="FunCoup" id="E3K8T9">
        <property type="interactions" value="359"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K8T9"/>
    <dbReference type="GeneID" id="10539661"/>
    <dbReference type="KEGG" id="pgr:PGTG_06601"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06601"/>
    <dbReference type="eggNOG" id="KOG4020">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054098_0_0_1"/>
    <dbReference type="InParanoid" id="E3K8T9"/>
    <dbReference type="OrthoDB" id="311633at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11000">
        <property type="entry name" value="Fe-S cluster assembly protein Dre2, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03115">
        <property type="entry name" value="Anamorsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007785">
        <property type="entry name" value="Anamorsin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046408">
        <property type="entry name" value="CIAPIN1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031838">
        <property type="entry name" value="Dre2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13273">
        <property type="entry name" value="ANAMORSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13273:SF14">
        <property type="entry name" value="ANAMORSIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05093">
        <property type="entry name" value="CIAPIN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16803">
        <property type="entry name" value="DRE2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3 9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0408">Iron</keyword>
    <keyword evidence="7 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe-S cluster assembly protein Dre2 N-terminal" evidence="12">
        <location>
            <begin position="93"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Anamorsin C-terminal" evidence="11">
        <location>
            <begin position="250"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Fe-S binding site B" evidence="9">
        <location>
            <begin position="316"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Cx2C motif 1" evidence="9">
        <location>
            <begin position="316"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Cx2C motif 2" evidence="9">
        <location>
            <begin position="327"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="316"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF16803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP80645.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="356" mass="38087" checksum="074B122B16EFBA90" modified="2012-04-18" version="2">MTRFLELVSVSGSSSGVRFGTSRAFSPAKNKLWAVNSAGEQTTTTTTTNTPKAQAVKKDKMTITDNHPAQTVLLIGSPRSIKDGTYTELITRFDNQGPSTIIEKQLCDRISDGATSLSQSHFDAIHLVVDANDLSLANSSHSPASFLDLIVRALKPLGQLTWSTQSGLSEIESTLKQSSVMADVQVVSGPNESQLVRATKSASTTSNSVLISLPKKSIKASLWSFTTAQPEMIDDSSLLTEEDLKKPSTMTTEDCNPKKAKKACKNCTCGLRELELAQDNDLPAHLKAPGSELPSQPTDPSKLVVNGSAKTFTSSCGSCYLGDAFRCSSCPYLGMPAFEPGQQVKLTAEMGDDIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAA4</accession>
    <name>E3KAA4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06882</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325680.1">
        <property type="nucleotide sequence ID" value="XM_003325632.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KAA4"/>
    <dbReference type="GeneID" id="10536748"/>
    <dbReference type="KEGG" id="pgr:PGTG_06882"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06882"/>
    <dbReference type="HOGENOM" id="CLU_1078245_0_0_1"/>
    <dbReference type="InParanoid" id="E3KAA4"/>
    <dbReference type="OMA" id="GWPATIP"/>
    <dbReference type="OrthoDB" id="2507047at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173366" evidence="1">
        <location>
            <begin position="21"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="243" mass="23134" checksum="9F45A341E8536E3E" modified="2011-01-11" version="1">MRVTCIFVSVAVLFAGHAHSLPILGLPSLPLVGGLTNGLGGVPLVGGLGNAAAPLTSGLTSKLGGLSHTVSPVASGLTGDVAPVTSSLGGVTQNVAPVTSDLTGLTGGLPDLQNAASGLTGELAPAASGLTSKLNGLTDDVAPAASGLNGLTGGLPDIQNVSSGLPVGGLLSSLIKRDPQFGGGLPIIGSMGSLPGLSSLPSMDSLPIPSMDSLPGLSSLGSLPGLSSMGSGLPGLDSLSGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAG7</accession>
    <name>E3KAG7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_07018</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81397.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325816.2">
        <property type="nucleotide sequence ID" value="XM_003325768.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KAG7"/>
    <dbReference type="GeneID" id="10537051"/>
    <dbReference type="KEGG" id="pgr:PGTG_07018"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07018"/>
    <dbReference type="HOGENOM" id="CLU_154040_0_0_1"/>
    <dbReference type="InParanoid" id="E3KAG7"/>
    <dbReference type="OrthoDB" id="10338889at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173165" evidence="2">
        <location>
            <begin position="28"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="84"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="125"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP81397.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="138" mass="14712" checksum="511F1337BB48993E" modified="2012-04-18" version="2">MLTSLSFLRVACCLFLICVYVPPGVSASMWDSTGAEKIAEGAHIGRKRPASSVDESSSYQMARLDPSSQAQLAVSSFRRNDDMVFRDGFGTHQEPLAGQPPGSQNSNLHGSMENDLPGRAESIGLGTSSHTKHSGQSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAN2</accession>
    <name>E3KAN2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">proteasome endopeptidase complex</fullName>
            <ecNumber evidence="3">3.4.25.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_07060</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.4.25.1" evidence="3"/>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325858.1">
        <property type="nucleotide sequence ID" value="XM_003325810.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KAN2"/>
    <dbReference type="FunCoup" id="E3KAN2">
        <property type="interactions" value="391"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KAN2"/>
    <dbReference type="MEROPS" id="T01.A02"/>
    <dbReference type="GeneID" id="10537096"/>
    <dbReference type="KEGG" id="pgr:PGTG_07060"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07060"/>
    <dbReference type="eggNOG" id="KOG0173">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_3_0_1"/>
    <dbReference type="InParanoid" id="E3KAN2"/>
    <dbReference type="OMA" id="KQHLFRH"/>
    <dbReference type="OrthoDB" id="429533at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034515">
        <property type="term" value="C:proteasome storage granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010499">
        <property type="term" value="P:proteasomal ubiquitin-independent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03763">
        <property type="entry name" value="proteasome_beta_type_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000005">
        <property type="entry name" value="Proteasome subunit beta type-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024689">
        <property type="entry name" value="Proteasome_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF4">
        <property type="entry name" value="PROTEASOME SUBUNIT BETA TYPE-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12465">
        <property type="entry name" value="Pr_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="8 11" id="KW-0647">Proteasome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0888">Threonine protease</keyword>
    <feature type="domain" description="Proteasome beta subunit C-terminal" evidence="10">
        <location>
            <begin position="234"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF12465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP81439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="293" mass="30972" checksum="7D22D92409658225" modified="2011-01-11" version="1">MTVVDIEPNSNGFDFSNHLRNSHLTSASGPSKLSLPKATSTGTTIVGLVWEGGICLGADTRATEGPIVADKNCEKIHFISDNIRCCGAGTAADTEFVTAMISSNIQLHALSTGRQPRVVTAMTMLKQHLYKYQGHVGAALVIGGVDPTGPHLFTVAPHGSTDKLPYVTMGSGSLAAMAVFETKWKPKMARQEAIDLVVQAIYAGIFNDLGSGSNCDVCVIEASGTEMLRNYAMPNERVAKELNYTMRRGTTAWIKQDIKKLIVSESVTRIGGGVVEGGPGTGAGAAEGMELDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAP0</accession>
    <name>E3KAP0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Sister chromatid cohesion protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_07068</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the rad21 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81447.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325866.2">
        <property type="nucleotide sequence ID" value="XM_003325818.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KAP0"/>
    <dbReference type="FunCoup" id="E3KAP0">
        <property type="interactions" value="300"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KAP0"/>
    <dbReference type="GeneID" id="10537185"/>
    <dbReference type="KEGG" id="pgr:PGTG_07068"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07068"/>
    <dbReference type="eggNOG" id="KOG1213">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_431881_0_0_1"/>
    <dbReference type="InParanoid" id="E3KAP0"/>
    <dbReference type="OrthoDB" id="10071381at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030892">
        <property type="term" value="C:mitotic cohesin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990414">
        <property type="term" value="P:replication-born double-strand break repair via sister chromatid exchange"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.580">
        <property type="entry name" value="Structural maintenance of chromosome 1. Chain E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039781">
        <property type="entry name" value="Rad21/Rec8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006909">
        <property type="entry name" value="Rad21/Rec8_C_eu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006910">
        <property type="entry name" value="Rad21_Rec8_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023093">
        <property type="entry name" value="ScpA-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585:SF69">
        <property type="entry name" value="FI11703P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12585">
        <property type="entry name" value="SCC1 / RAD21 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04824">
        <property type="entry name" value="Rad21_Rec8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04825">
        <property type="entry name" value="Rad21_Rec8_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad21/Rec8-like protein N-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="Rad21/Rec8-like protein C-terminal eukaryotic" evidence="5">
        <location>
            <begin position="677"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="231"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="349"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="505"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="576"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="252"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="505"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="613"/>
            <end position="622"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP81447.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="737" mass="80970" checksum="68040296E30A9F63" modified="2012-04-18" version="2">MFFSSEMLSKRGPLAKVWLAAHVERKVSKAQTLQTSIPSTVTVILEPASTMVSAPPLALRLSGQLLLGIARIYSKQAKYLLEDCSEASDKIRSAFRSETIQSMIDEPAGEDHLILPAQPNVTGRDAINLRSAANRDLFDFELEFGEGFGYGFGDGWEDVNQANGTNDIVPIVSTSRSRIDGNLADITLTEHQSFMLGGDQSEERLDDLRSDDMNLMADADPLDLGLVSATEGLDSEARAHRRNRQTRNGSTIEEEGQLADETMEMEVGRDAPGTADRRSARDSLLPEQLGDLSVDKSYDVPSGMGDTSFDLGGPAPDFEPTGLDVEPGVDLNQDTGIQDITMADGQQDLGAGDGQMTPRATTPVPNPMLDQLDVTPRTSHHLAPHLTSPAVGAEGDDQQPPKKKKKHQLVDRVTELAAADHLPSSLNTGNDHQSTLDPPRFLPKDRYQLQHHRIMLDPSCHEFLPQLLDVDGERWLMAAPAGLCKELQELFKFPAKASAVSVRERGMKRLRDDEQEREELESEHGRRAPSAGIGEGDFSRIDTMGDETFDLMGHREDDQGAPFGADDGFQFELADKSHAEQESSAPNDLIQPNEEPRFDADLEQPLPPLPAEPEVRRQRAEGEVSKLDVFDDSGDTQSQSQAVESIELTGTANRTKWSKNTVKALTVLKTELADGPDVLKFKEVSDKASRRAGAAFFFELLVLGTRDCLKLSQSEPFGEIEVESQDRLWEVLEEQAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3KC90</accession>
    <name>E3KC90_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_08097</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="2"/>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EFP81848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326267.1">
        <property type="nucleotide sequence ID" value="XM_003326219.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KC90"/>
    <dbReference type="STRING" id="418459.E3KC90"/>
    <dbReference type="GeneID" id="10533885"/>
    <dbReference type="KEGG" id="pgr:PGTG_08097"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08097"/>
    <dbReference type="HOGENOM" id="CLU_966878_0_0_1"/>
    <dbReference type="InParanoid" id="E3KC90"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000002">
        <property type="entry name" value="Putative Ubiquitin-protein ligase E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044611">
        <property type="entry name" value="E3A/B/C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700:SF2">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="6">
        <location>
            <begin position="71"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP81848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="288" mass="33018" checksum="6519A67BDA379832" modified="2011-01-11" version="1">MSQRHSAEAVEKPLQLALDLEVCRFIWRERKRHWPDCYTALQRSGKKNLCAHCCTALSAAERLQRLHPHPHPLAGIHGYLPFKWAEIYPNPHSYSCQPLSLNWKKFLGRVLGKALYKGILININFADFFLNKCLGKQSYLDDLASLDPEFYQGLIFLKYHKGGIEADLLLNFTVTNNEFDVLETIELIPEGLKTSVTAQNRINYIYLMLNYKLKIQLESQLDHLWELGQVTSDDQNSLRATASVWVGKMEVQTLPFYTIRNKTPKLSLESFDNVIGLVGSGKTLFDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCS1</accession>
    <name>E3KCS1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_07620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326642.1">
        <property type="nucleotide sequence ID" value="XM_003326594.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCS1"/>
    <dbReference type="GeneID" id="10529595"/>
    <dbReference type="KEGG" id="pgr:PGTG_07620"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07620"/>
    <dbReference type="HOGENOM" id="CLU_1993730_0_0_1"/>
    <dbReference type="InParanoid" id="E3KCS1"/>
    <dbReference type="OrthoDB" id="10502625at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP82223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="125" mass="14764" checksum="B1DE80936032E286" modified="2011-01-11" version="1">MAELRWYIWKPGGKSNFYHPGFTTRGGDALMTHLLMVSKEERTTTKKVKNKPLDKWQEWRRKFWPRGLVKDHQVKKFGEIFHEFVEGSVSYGFWKRPKLVVFTTYTSIGQDEIMCFANKAGVKQC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDE9</accession>
    <name>E3KDE9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_07555</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82158.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326577.2">
        <property type="nucleotide sequence ID" value="XM_003326529.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDE9"/>
    <dbReference type="SMR" id="E3KDE9"/>
    <dbReference type="FunCoup" id="E3KDE9">
        <property type="interactions" value="446"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KDE9"/>
    <dbReference type="GeneID" id="10529530"/>
    <dbReference type="KEGG" id="pgr:PGTG_07555"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07555"/>
    <dbReference type="eggNOG" id="KOG0106">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_34_2_1"/>
    <dbReference type="InParanoid" id="E3KDE9"/>
    <dbReference type="OrthoDB" id="1099063at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000381">
        <property type="term" value="P:regulation of alternative mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12339">
        <property type="entry name" value="RRM2_SRSF1_4_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000533">
        <property type="entry name" value="Chromosome 16, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050374">
        <property type="entry name" value="RRT5_SRSF_SR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003">
        <property type="entry name" value="RNA RECOGNITION MOTIF RRM DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23003:SF51">
        <property type="entry name" value="SERINE-ARGININE PROTEIN 55"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="6"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="94"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="162"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="163"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP82158.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="258" mass="29621" checksum="39848737C4AEE30E" modified="2012-04-18" version="2">MASAGRRIYVGRIPPDASRTDVEKYFGRYGTLMDVRIMAGFGFLEYDSVRDAEDAVHDLNGRDFMGERLIVEFAKAPRGRDIHSGGHGPRRGGFRLLVKGLSHETSWQDLKDFARQAGNVTRADVDRNMPGEGLIEFASQDDADNAIRKLDGTELKGMVVTLIEDRPGGGGGGRRDRSRSPAYRDSRDRDRSPRDRSRDRERDRSPRDRRYDRERRRSPPPRDDRDRERDRGYDRKERDRSPRRDVRADSVSKKSPSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDM9</accession>
    <name>E3KDM9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_08421</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326884.1">
        <property type="nucleotide sequence ID" value="XM_003326836.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDM9"/>
    <dbReference type="STRING" id="418459.E3KDM9"/>
    <dbReference type="GeneID" id="10529844"/>
    <dbReference type="KEGG" id="pgr:PGTG_08421"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08421"/>
    <dbReference type="HOGENOM" id="CLU_140513_0_0_1"/>
    <dbReference type="InParanoid" id="E3KDM9"/>
    <dbReference type="OMA" id="VCERTDI"/>
    <dbReference type="OrthoDB" id="2512749at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171922" evidence="2">
        <location>
            <begin position="22"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="112"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP82465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="159" mass="16609" checksum="D0617186B2C80243" modified="2011-01-11" version="1">MLKSTLLASLLSIASLQTVSARQAPPLLAKRGVIGVDVNFCPHWDKLEACRGGSHACCRQFCASYRVSIETEAATQFCNPGNAPKKPDPSPPPPPTPPPPAPSPPPPPPPTVQVAPAPIVIGTAPPPPPPTVYYAQPAPQVLYSQPVPYAPAPVLVQSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDP3</accession>
    <name>E3KDP3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_08435</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82479.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326898.2">
        <property type="nucleotide sequence ID" value="XM_003326850.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDP3"/>
    <dbReference type="GeneID" id="10529858"/>
    <dbReference type="KEGG" id="pgr:PGTG_08435"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08435"/>
    <dbReference type="HOGENOM" id="CLU_108601_0_1_1"/>
    <dbReference type="InParanoid" id="E3KDP3"/>
    <dbReference type="OrthoDB" id="2496900at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP82479.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="207" mass="23432" checksum="ED232B320C20CBE2" modified="2012-04-18" version="2">MPIATSSHPANRAPLSKAYIMKQEMDYSKGSLRIYGPDGTVLYRFTRHIQDPITGYTTVVLMSPASDALIVLESSNNLCGHKTTYTEMKVPGSRKRQFTIDPEGLLKDQWTFNYVDGTNKQQNFKFDRNYANKEEPIPSGKVYQQIKGENGELVAEFEDQKRTDSWLTTSSTREVDTYTLFCTADSPQVELVLLMGLVMNRVHDCGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDQ2</accession>
    <name>E3KDQ2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dipeptidyl-peptidase V</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_08444</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S9C family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82488.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326907.2">
        <property type="nucleotide sequence ID" value="XM_003326859.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDQ2"/>
    <dbReference type="FunCoup" id="E3KDQ2">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KDQ2"/>
    <dbReference type="GeneID" id="10529867"/>
    <dbReference type="KEGG" id="pgr:PGTG_08444"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08444"/>
    <dbReference type="eggNOG" id="KOG2100">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008615_0_1_1"/>
    <dbReference type="InParanoid" id="E3KDQ2"/>
    <dbReference type="OrthoDB" id="416344at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001375">
        <property type="entry name" value="Peptidase_S9_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776:SF13">
        <property type="entry name" value="DIPEPTIDYL-PEPTIDASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42776">
        <property type="entry name" value="SERINE PEPTIDASE S9 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00326">
        <property type="entry name" value="Peptidase_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Peptidase S9 prolyl oligopeptidase catalytic" evidence="5">
        <location>
            <begin position="538"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00032829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP82488.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="613" mass="67499" checksum="77BBC13AC8A5A910" modified="2012-04-18" version="2">MVNNHSTNILEPVSLAELPKPGPYIPNPSAQSALSVIKTYNHARQSTVTSLITIDLTQVQDQQASSHTTVLTDLAYAEAVWLDSNSILYLRPPGAQEAGPDLDPLISTKLAREHLKKRLEELADDQGKGIELWAIRIGGKGDKKAYRVGSLPVPIGNIKFQSFSSSSAGVLGFSVDVYPPYDTEALKNLHKLERSFEEAAEGSTGQTYDSVYVRHWDEWIDQHGKEARLFVIDLEQKEDHTWFIPDSHQPRSLFPSLSVPVGPSGSSSDFDLSSSSAVVTAKDPDVNPAWHTRQNIYIMPLYPRTKLLPKPLQLTTGDQGGTSNPVFSPSAKNANGCDQGKVAWLEMNCDGYESDQNQVVVYNLESRTRSYFAKSWDRSPSKIIWGKDDDELYLLAEEHGRVKVFYLSLESSEGPECLTDENSASYLAYLGKKSAQDAEKPAARLLLTMSSLAFGPTPYILDLALPDHCTKCTKVGSLCDLSGTTLDEGEDFWFHGAENRSVHGILIRPPGFVADLGQKWPLMFLIHGGPQGAWDKTWSLRWNANTFAGAGYIVAMINPTGSTGYGQKFTDSIKQDWGGKPYKDLVAGYQYLLKTYAEIDGSRTAALGKSHAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDS2</accession>
    <name>E3KDS2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08464</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82508.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326927.2">
        <property type="nucleotide sequence ID" value="XM_003326879.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDS2"/>
    <dbReference type="GeneID" id="10529887"/>
    <dbReference type="KEGG" id="pgr:PGTG_08464"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08464"/>
    <dbReference type="HOGENOM" id="CLU_536523_0_0_1"/>
    <dbReference type="InParanoid" id="E3KDS2"/>
    <dbReference type="OrthoDB" id="2510938at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82508.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="508" mass="58766" checksum="930D4F22B4C0632F" modified="2012-04-18" version="2">MADDFGGWKFHDEIHDHVPNSAFKIASLDSDSSKEITPDSLEELEGKNTKPLHTGHKNSPGIFFHPLAHFDTPDLADQELLVLNKAQYFPAENTNSPATISHSPTGLASESTASLREEPCFPNSNANPPVVHEQTPQLTSSSHPRPHNKSKRPRTKRYRHPEVQVGNPPAPLDWNPKPRFEKTQHFSEKNDDYFKIFGMEDEEFDALGQNFLTTLQRKSTKLNLEVQAESSEFFKRLRGGKTMVWIFGNTVYRAEPEEQRLKSKYASTLSEFEKIFALKIESLINQGQSSTSEERNTRGVYQLVLQFSDILWINNLRLLDSLGLKDEKYYLIAHALVYRWLLRCTQDDLEHLVLEIFKTALTLDPSKERIYLILLLATYYKTVNEEKWELVLGNDSRFLSHIVKNQTEAFHSVTRTSRAKMRLLKLFPWVGNFHEFNTYELLSGLRNHRNHRVDLDSYVQVEPRLEKGQYEEIGEIPSELTETVMEKSLTIEFTQTMLDVEAFRMQYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KEQ3</accession>
    <name>E3KEQ3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08953</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82757.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327176.2">
        <property type="nucleotide sequence ID" value="XM_003327128.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KEQ3"/>
    <dbReference type="GeneID" id="10530140"/>
    <dbReference type="KEGG" id="pgr:PGTG_08953"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08953"/>
    <dbReference type="HOGENOM" id="CLU_900579_0_0_1"/>
    <dbReference type="InParanoid" id="E3KEQ3"/>
    <dbReference type="OrthoDB" id="2513320at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="252"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82757.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="309" mass="34857" checksum="13564BF6682CCB26" modified="2012-04-18" version="2">MLIKLEQGSTGACLVMEKQAHQTTRANLGLGLNPTNLPQSPLQFLRTPLITLATSNLGVIPIGMFIMLEKLRSLITQYPINILYINVQSIRKLFNNNLSINSLSINHWPINILPIHTGTRFLRPFSILQDMKPTDLLSLREINTAKENIHSTEVKNNGKARKYVEIGDDEKHPHYKLLPDDLHPEYVDHLSLPADLLEGIYDDIHKTDKVQNNEILKESPKNPVNNVENILESNQKNPEAMEGNAIHSEIQSKSDLDDHEQKSHIPEITSPSKQKDMSVEEKQDQSKGFLAHNGKLYIHNMAGGGVHMD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KFI0</accession>
    <name>E3KFI0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09975</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178284">
        <property type="protein sequence ID" value="EFP83007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327426.1">
        <property type="nucleotide sequence ID" value="XM_003327378.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KFI0"/>
    <dbReference type="GeneID" id="10544719"/>
    <dbReference type="KEGG" id="pgr:PGTG_09975"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09975"/>
    <dbReference type="HOGENOM" id="CLU_1409435_0_0_1"/>
    <dbReference type="InParanoid" id="E3KFI0"/>
    <dbReference type="OMA" id="FAQNMGQ"/>
    <dbReference type="OrthoDB" id="2498105at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173312" evidence="1">
        <location>
            <begin position="22"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="186" mass="20166" checksum="E5F1B57395322106" modified="2011-01-11" version="1">MVAFNFGLLATLFLVIALVVAHPNNEGLVARDMSPEAKTKGRVSDKLDSLGKNVKWLNDAVQQQNLNSRVARERVAGYYQLFRDSFQYANDCGRLCFQSGSVVNVSAYKAFTQLDQLAKSVASKYGSGASTVMSPFSAYDTLVARTINGFAQEGTPAYNLLPPQFADSMAQVGFPQTAAAAHQIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3KFU0</accession>
    <name>E3KFU0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_09178</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EFP83225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327644.1">
        <property type="nucleotide sequence ID" value="XM_003327596.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KFU0"/>
    <dbReference type="GeneID" id="10544938"/>
    <dbReference type="KEGG" id="pgr:PGTG_09178"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09178"/>
    <dbReference type="HOGENOM" id="CLU_1390849_0_0_1"/>
    <dbReference type="InParanoid" id="E3KFU0"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP83225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="196" mass="22396" checksum="02C1C80702EBD312" modified="2011-01-11" version="1">MGPGTRLYSSYAREPSVKECRSADFVRRVSRKGDPTVTWKRFDVISRDFKNFKLNADRFGGVRSLIDHIVRWTVSSLLAYVPHYWSKDGRRRSPKSTFQPSCGPSKKASIHTQLHYRYEIPIVRMCPIYAEVKPCRVSSGSFKSSVTPGRLSHRSSISSPKQFPAAKWNKYQLGVQRLITSDVHIGFIPKTQSEIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>E3KFV0</accession>
    <name>E3KFV0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_09188</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily.</text>
    </comment>
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        <property type="protein sequence ID" value="EFP83235.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327654.2">
        <property type="nucleotide sequence ID" value="XM_003327606.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KFV0"/>
    <dbReference type="GeneID" id="10544948"/>
    <dbReference type="KEGG" id="pgr:PGTG_09188"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09188"/>
    <dbReference type="eggNOG" id="ENOG502S21N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051542_1_0_1"/>
    <dbReference type="InParanoid" id="E3KFV0"/>
    <dbReference type="OrthoDB" id="2094832at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051654">
        <property type="entry name" value="Meroterpenoid_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35897">
        <property type="entry name" value="METHYLTRANSFERASE AUSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35897:SF1">
        <property type="entry name" value="METHYLTRANSFERASE AUSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP83235.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="312" mass="35525" checksum="6906D9836E40C610" modified="2012-04-18" version="2">MNLSDKDTSLPFSPSTPPDNCGIKMTDVSVPLKGVQAVYPLDDSVQAQDLKFFKKSTGIKDAADLKKHIMEIRETAYKSFPYPCIWGFEFLKGKTTSHPFYPQVKENQLSSKHQKFFIDLGTFVGVDLRQVVNDGWNPNNVLGVDIHPEWRDLGHQLFKDGNEALPFYLGDILQVKTLNDSPSSEARPVLDLRDLQDLNKLKGRVSFISANQLFHLFSKGDQKELAERCGRLLSHEPGAAIFGTQLGLPEEKLETYKLYLHSPESWERMWKEVLGENILVTVKLEEADTEHSYHSFSGVESLYWLYWSVART</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KFY1</accession>
    <name>E3KFY1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09097</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EFP83144.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327563.2">
        <property type="nucleotide sequence ID" value="XM_003327515.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KFY1"/>
    <dbReference type="GeneID" id="10544857"/>
    <dbReference type="KEGG" id="pgr:PGTG_09097"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09097"/>
    <dbReference type="HOGENOM" id="CLU_1619868_0_0_1"/>
    <dbReference type="InParanoid" id="E3KFY1"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83144.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="164" mass="16846" checksum="A1FC87E02E3FD2E2" modified="2012-04-18" version="2">MSDTMWNLPGDMHGNNAAAHTDGQDELEDPIKSLLDDPGATPLALPGNSASELPDDLIAETQSMNPLPNPSQSINPPASQLTNPPARLNCSNLPPLSDAQLAPLAQVDSPSPPPAYESIPAQATWPTPSRATRTASVGAIPAATGSGVMPSTWHPLAKSGAPAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3KFZ3</accession>
    <name>E3KFZ3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_09217</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EFP83264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327683.1">
        <property type="nucleotide sequence ID" value="XM_003327635.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KFZ3"/>
    <dbReference type="STRING" id="418459.E3KFZ3"/>
    <dbReference type="GeneID" id="10544978"/>
    <dbReference type="KEGG" id="pgr:PGTG_09217"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09217"/>
    <dbReference type="eggNOG" id="ENOG502T18T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1603567_0_0_1"/>
    <dbReference type="InParanoid" id="E3KFZ3"/>
    <dbReference type="OMA" id="ITACAMC"/>
    <dbReference type="OrthoDB" id="2495271at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:001065">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051826">
        <property type="entry name" value="E3_ubiquitin-ligase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765">
        <property type="entry name" value="RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="116"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="50"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="66"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP83264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="165" mass="18432" checksum="32934866B976BC41" modified="2011-01-11" version="1">MSRSPVASRNPFVPPPNTVTFSAASWSSQGLTFNVLFSIMDRSRGFPVEQDFQSTSPDSLWPISDQPWTSTLSDTASSPNGPEPTESQHNIDLVLNSIGQALSAFSLSLQHAITNCGMCLEDYKANDQVLVLPCHPSHFFHRHCLRDWFKEYLNCPMCRKKFGPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>E3KG82</accession>
    <name>E3KG82_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Isoleucine--tRNA ligase, cytoplasmic</fullName>
            <ecNumber evidence="2">6.1.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Isoleucyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_09131</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Ile) + L-isoleucine + ATP = L-isoleucyl-tRNA(Ile) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:9666"/>
            <dbReference type="Rhea" id="RHEA-COMP:9695"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78528"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.5" evidence="2"/>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EFP83178.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327597.2">
        <property type="nucleotide sequence ID" value="XM_003327549.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KG82"/>
    <dbReference type="FunCoup" id="E3KG82">
        <property type="interactions" value="615"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KG82"/>
    <dbReference type="GeneID" id="10544891"/>
    <dbReference type="KEGG" id="pgr:PGTG_09131"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09131"/>
    <dbReference type="eggNOG" id="KOG0434">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001493_1_0_1"/>
    <dbReference type="InParanoid" id="E3KG82"/>
    <dbReference type="OrthoDB" id="1706657at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004822">
        <property type="term" value="F:isoleucine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006428">
        <property type="term" value="P:isoleucyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07961">
        <property type="entry name" value="Anticodon_Ia_Ile_ABEc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00818">
        <property type="entry name" value="IleRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.740.10:FF:000044">
        <property type="entry name" value="Isoleucine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000176">
        <property type="entry name" value="Isoleucine-tRNA ligase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000004">
        <property type="entry name" value="Isoleucyl-tRNA synthetase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000023">
        <property type="entry name" value="Isoleucyl-tRNA synthetase,cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02003">
        <property type="entry name" value="Ile_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033709">
        <property type="entry name" value="Anticodon_Ile_ABEc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002301">
        <property type="entry name" value="Ile-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023586">
        <property type="entry name" value="Ile-tRNA-ligase_type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00392">
        <property type="entry name" value="ileS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42780:SF1">
        <property type="entry name" value="ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42780">
        <property type="entry name" value="SOLEUCYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19302">
        <property type="entry name" value="DUF5915"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00984">
        <property type="entry name" value="TRNASYNTHILE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="12">
        <location>
            <begin position="22"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="13">
        <location>
            <begin position="705"/>
            <end position="864"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00069879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EFP83178.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1100" mass="126905" checksum="C17F13B2D31C3ECB" modified="2012-04-18" version="2">MENFPPHDPNSPFSFPKEEEKVLEYWNSIDAFQTSITQSKLELEAGKRKRWNFYDGPPFATGLPHYGHLLMGTVKDIVTRFAHSDGNYVERRFGWDCHGLPVEHEIDKKLQIQSKQDVMDMGIPKYNAECRAIVMRYSKEWRATIERMGRWIDFDNDYKTLNVSYMESIWWVFKQLWEKDQVYRASRVMPYSTSCTTPLSNFEAGSDYRDVNDPAVTISFPLIDDPSTCLLAWTTTPWTLPSNLAVCVHPEMTYIKIFDEQHQKNFIICEKLLGTLYKDPKKAKFKKVATYLGKDLEGWKYEPLMDYFYDQYKDTAFKVVTDTYVTSDSGTGIVHQAPAFGADDHEVCKRHGVVPADVIPPCPIDDGGRFTSDVPDFQGQHVKEADPHITKFLKQKGRVIVQSQIKHSYPFCWRSGTPLIYRAIPAWFVRVQPVIDQLVENNRQTRWVPAAVGENRFQNWLANARDWNISRNRYWGTPLPIWVSDDYEEQICVGSIEELNELSGCGILKDLHRESVDNILIPSRMGKGMLKRVDEVFDCWFESGSMPYAQVHYPFENKEAFESAFPAQFIAEGMDQTRGWFYTLLVLSTHLFQKAPWQNLIVCGLVLAEDGKKMSKSLKNYPNPNVILDLYGADPLRLFLINSPVVRGENLRFREAGVKEVVSRVMLPWLNSYRFFLGQVALLKKVSGIDFEYDPKAKLSENVMDRWVLARCQGLIQYVRAEMEAYRLYTVVPQMLDLVDELTNWYIRFNRRRLKGENGPEDAVVALNTLFETLFTLCRTLSSFTPFLTENIYQGLRSFFPKGNLDLGYGDDLRSVHFLRFPEVRAEYFDPVIVRQVKHMQTVINLGRTIRERKTISLKTPLKELVVFHTDPEYHEDVKKLQNYVEDELNVHQITYSSDEAACGVKYKAGADWAVLGRRLKKDLAKVKRGLESTTSDQLKNYLESSRMTIEGIEIGPGDLTVSRYVDKEAGSSDESPTAPAYENNTDEDVVILLDCKLRPELESEGFARELMNRIQRLRKKAGAVQTDDLDVYYQFSEDSIGEANEFFEQVLATQGEVLCRVLRKVPEPDSLRDPSKPILAQELQERWGDQKVTLTLVRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGF2</accession>
    <name>E3KGF2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08563</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83377.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327796.2">
        <property type="nucleotide sequence ID" value="XM_003327748.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KGF2"/>
    <dbReference type="GeneID" id="10545093"/>
    <dbReference type="KEGG" id="pgr:PGTG_08563"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08563"/>
    <dbReference type="HOGENOM" id="CLU_095050_1_1_1"/>
    <dbReference type="InParanoid" id="E3KGF2"/>
    <dbReference type="OrthoDB" id="2498688at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173874" evidence="1">
        <location>
            <begin position="25"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83377.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="209" mass="22727" checksum="DCACAE1AEC1A9DE8" modified="2012-04-18" version="2">MIAYRCVLIVVISILLATTFPVDAKGCWNVNGDVNRKRNNKDCYAAMKKIIFDTSGKAPHLPTDEGVVKTISGGCSLIVKNPNHAIVTEPDIKVALEALFKKCPNIGGYVMFPNIPQASLEIRERAAAGSRYEAFNPDAELDKPYCYQAKGMPDIKANDDCIKAYEGLSTDSQGRISPTTNGAHVVFQSCYVSQTGQANIRTPLLLAND</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGN6</accession>
    <name>E3KGN6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08647</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83461.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327880.2">
        <property type="nucleotide sequence ID" value="XM_003327832.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KGN6"/>
    <dbReference type="STRING" id="418459.E3KGN6"/>
    <dbReference type="GeneID" id="10532138"/>
    <dbReference type="KEGG" id="pgr:PGTG_08647"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08647"/>
    <dbReference type="eggNOG" id="ENOG502R0EC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011151_1_0_1"/>
    <dbReference type="InParanoid" id="E3KGN6"/>
    <dbReference type="OrthoDB" id="2017782at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="12"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="511"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="308"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="522"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83461.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="646" mass="74677" checksum="DFBB135DB477C260" modified="2012-04-18" version="2">MRMAVEVAAVAQVAGKKRTQMEETVQDTLKPQFTNQNQNQNQNQNQNQNQNQNQNQKENGESESESEGEREERRETRSREGSGLCNSHLASAYFERLGLFRRMRTFASHPAPTHPDGRRSTDLEPYKDQRSFPQPGNQFMSLISRDLWLIYLMVLENDGRNWSQLLNSGNITGFLLNYSHYEINPSAISPGYPIERPEIAIALRLCHLFLDLQQEESESQPRSNSASEGAGYWTSEAGIETYLFALKPYTLAAHQYDVAYAPWSIRQLPIDFSSPDVSRVLQKKLTERELATLAPMRWGHSQTDTRRLNVTSNQTSINRSTSNGRASRIDNEPSSSSERGHLFQSPVQRYSELAYMGKLFRILPPLHSHVALLSFFWCNIRHNDSAETQGSEVEDQLEFFLRRMRSVLKMTSVEYDREFVRQTSCLDPFLSPGLQFDFYQGCFRGGWEGQFGFYDLSNYRLMLEGRIESVYEGRYGDQNQVFKVQEFIVRVRDDQPVEFLGDSREKYLEPRRSAGGFEAEEKDDRHHTRSEEPLESEIERLSQMKSLLYPTPIDSILVNYDPTEQYELQIHGAGHSSWGHFKVKGRVRAWDGMVTFLKIYDTGRNGRWLYRGYHTSGNMIVGRWRDTLTSVNLDGYEGAFVMFTRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHF0</accession>
    <name>E3KHF0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CTLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_09438</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FYV10 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83725.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328144.2">
        <property type="nucleotide sequence ID" value="XM_003328096.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHF0"/>
    <dbReference type="FunCoup" id="E3KHF0">
        <property type="interactions" value="466"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KHF0"/>
    <dbReference type="GeneID" id="10532412"/>
    <dbReference type="KEGG" id="pgr:PGTG_09438"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09438"/>
    <dbReference type="eggNOG" id="KOG0396">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027445_2_0_1"/>
    <dbReference type="InParanoid" id="E3KHF0"/>
    <dbReference type="OrthoDB" id="1933455at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034657">
        <property type="term" value="C:GID complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16659">
        <property type="entry name" value="RING-Ubox_Emp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013144">
        <property type="entry name" value="CRA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024964">
        <property type="entry name" value="CTLH/CRA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006595">
        <property type="entry name" value="CTLH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045098">
        <property type="entry name" value="Fyv10_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006594">
        <property type="entry name" value="LisH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12170:SF2">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN TRANSFERASE MAEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12170">
        <property type="entry name" value="MACROPHAGE ERYTHROBLAST ATTACHER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10607">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00757">
        <property type="entry name" value="CRA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00668">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50897">
        <property type="entry name" value="CTLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50896">
        <property type="entry name" value="LISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CTLH" evidence="3">
        <location>
            <begin position="176"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="322"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP83725.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="484" mass="53883" checksum="B889EE4BE4A5567C" modified="2012-04-18" version="2">MSSRIKHDDILLLEQPLLKVPTDALRSSLRSTQRVLERDFDRLNTSLKEAAGSSSRPIDGNDHQATVVDVEKGNETIKVIDGLLSKARGLKRKLDDTYNNPRTGMGPSLSRIKARIDHLQNLYSSEYSTDLAYKKFSQTRLDRHLVDFMLRSGHTQSAQSLSKVAGIEMLTDAPLFSELARIEKALTDHSCTEALAWCKENAAALKKTQSSLEFELRYQEFIELVKAKKFTEAISYSQKQLVPWQSTRLAEISQVMTLLAFDQRTRCPPYARLYDESRWVDLLTSFRSTLFALLSIPEQPFLHLSLSVGLASLKLPACYSDPKKPATSTTEDLEMTPPSFQTRLPNPGEFIGASSATTSLGNAAITTNSHLAPPSNYDPSLVQRMESDHRPSAQRNPDCPTCDPAGLGELAKECPWSHHVNSIIVCGLTGKVVNDGDGLAVLPNGRVYSRDGLERLACKDDGRVRCPRTGQVFNIEEMRRVFIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIG1</accession>
    <name>E3KIG1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_10464</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84086.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328505.2">
        <property type="nucleotide sequence ID" value="XM_003328457.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIG1"/>
    <dbReference type="STRING" id="418459.E3KIG1"/>
    <dbReference type="GeneID" id="10545182"/>
    <dbReference type="KEGG" id="pgr:PGTG_10464"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10464"/>
    <dbReference type="eggNOG" id="ENOG502R0XR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_934251_0_0_1"/>
    <dbReference type="InParanoid" id="E3KIG1"/>
    <dbReference type="OrthoDB" id="10394612at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173416" evidence="2">
        <location>
            <begin position="25"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="82"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP84086.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="296" mass="33401" checksum="0037FEFAC8AA0A41" modified="2012-04-18" version="2">MILPSAITLAIGLFFGQFAASTSANSLSARHEPVGPQALKGATLGRRNYDHHHSNSRKGGKDHVGYGYMSKGYSGNVNYNQDSGKGSYNSGKGSYDSGKGSYDSGKGSYNSGKSSYDSHQDYAIEKKSFNYESDKKESSSVKKKYEYKDNNYGSDKGVSNYSDKKEGYTKYHKRSYKDDGKYDMKNYKDEGKSDEKKDYKDEGKHEEKKDYKVEGKYNEKKYYKVDGKYNEKKSSEGHQESGKKNDSTEKHNSQNSKYDSSKHHGGSDYSEKKGDEDDRKHGGYSNDYKQDAKGHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIL2</accession>
    <name>E3KIL2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase S54 rhomboid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_10515</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84137.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328556.2">
        <property type="nucleotide sequence ID" value="XM_003328508.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIL2"/>
    <dbReference type="STRING" id="418459.E3KIL2"/>
    <dbReference type="GeneID" id="10545315"/>
    <dbReference type="KEGG" id="pgr:PGTG_10515"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10515"/>
    <dbReference type="eggNOG" id="KOG4463">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_057574_2_2_1"/>
    <dbReference type="InParanoid" id="E3KIL2"/>
    <dbReference type="OrthoDB" id="272778at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1540.10">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035952">
        <property type="entry name" value="Rhomboid-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43066">
        <property type="entry name" value="RHOMBOID-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43066:SF21">
        <property type="entry name" value="UBIQUITIN-ASSOCIATED DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144091">
        <property type="entry name" value="Rhomboid-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="51"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="161"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP84137.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="238" mass="26670" checksum="B2332A52DA96E751" modified="2012-04-18" version="2">MGASSTFAHAPLSRIIIFGTSILSLLLSGHKHYLDLPLTPHLTRDFQFWRILTHHTACSNSADLFLIVLILWNVSVTVERRFGTVKYASYLIVTVVVGSLLELMGLLISHSIMGYRAIPSGPFMVTFAIVYQHHAIVPSLYDYRIGRVHLDSHSLVPDILSLLLGLFNPLSSLVGILVGALYRTDQLGLARWRLRNSFLLSRLTSRSSDRRRKPRPTHASLEDDPRFEATIAPSRSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIW6</accession>
    <name>E3KIW6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10619</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328660.1">
        <property type="nucleotide sequence ID" value="XM_003328612.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIW6"/>
    <dbReference type="GeneID" id="10545502"/>
    <dbReference type="KEGG" id="pgr:PGTG_10619"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10619"/>
    <dbReference type="HOGENOM" id="CLU_1355225_0_0_1"/>
    <dbReference type="InParanoid" id="E3KIW6"/>
    <dbReference type="OrthoDB" id="10302575at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="202" mass="22207" checksum="254799EB8465A775" modified="2011-01-11" version="1">MSGSVERSTQEELSEMMTTRLSLSEPRLNNPFEPSGTSDEGMSDGASRMPMEIDDTEQAEAISADRSTTTKTEAEMMEIVDPASGPMDRRGAGSPAGKKAPRRSTRAGSVPARPRSQHGHPFRLQRTVPQLLRGSWIHPRLQHSFAPRPPLNPLPTIIGIDQMQLEPASSALLRGSTALRRSTRPALRHDSHITRDRANQAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJ87</accession>
    <name>E3KJ87_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_10082</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178290">
        <property type="protein sequence ID" value="EFP84362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328781.1">
        <property type="nucleotide sequence ID" value="XM_003328733.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJ87"/>
    <dbReference type="STRING" id="418459.E3KJ87"/>
    <dbReference type="GeneID" id="10545789"/>
    <dbReference type="KEGG" id="pgr:PGTG_10082"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10082"/>
    <dbReference type="eggNOG" id="ENOG502SC67">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_121574_4_1_1"/>
    <dbReference type="InParanoid" id="E3KJ87"/>
    <dbReference type="OMA" id="ENHLMHR"/>
    <dbReference type="OrthoDB" id="2505784at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.150.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043451">
        <property type="entry name" value="Myocardin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004018">
        <property type="entry name" value="RPEL_repeat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22793:SF12">
        <property type="entry name" value="MYOCARDIN-RELATED TRANSCRIPTION FACTOR, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22793">
        <property type="entry name" value="MYOCARDIN-RELATED TRANSCRIPTION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02755">
        <property type="entry name" value="RPEL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00707">
        <property type="entry name" value="RPEL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51073">
        <property type="entry name" value="RPEL"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="RPEL" evidence="4">
        <location>
            <begin position="11"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="repeat" description="RPEL" evidence="4">
        <location>
            <begin position="55"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP84362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="87" mass="9758" checksum="3333516BA1C39BAC" modified="2011-01-11" version="1">MDSLHGLQKTRKLEKLLAERPDKTELIEKNVLKPGNLDPSQQAKQAELSKNQLEDKLNAALAHRPEPEKLVQEGILTEEETAALKAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJS9</accession>
    <name>E3KJS9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10713</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84554.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328973.2">
        <property type="nucleotide sequence ID" value="XM_003328925.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJS9"/>
    <dbReference type="GeneID" id="10546143"/>
    <dbReference type="KEGG" id="pgr:PGTG_10713"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10713"/>
    <dbReference type="HOGENOM" id="CLU_056602_1_0_1"/>
    <dbReference type="InParanoid" id="E3KJS9"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="153"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84554.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="231" mass="25893" checksum="8E188C32BE6BE7C3" modified="2012-04-18" version="2">MPSEEPIPNLGSSPQSSHSEHEGSHEENITDDGQDVEVSVFFTIYVNMPPRQGRRGMVRVPPRCFKHHKGCSIQLHSRSATLDEVQHKISDILNARCGNLGNMVLADFESESPKITWKVYVSNGASGLFSQRNNMILTPGIFEEWKDEIRHSRRGQAKISIQQPKPSQESTADEEAAVERALQEMHGANGGPNENRDQPGNITESNERQETPPLNDFALSATIRELYNIHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KK35</accession>
    <name>E3KK35_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_10819</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP84660.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003329031.2"/>
    </dbReference>
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    <dbReference type="GeneID" id="10546329"/>
    <dbReference type="KEGG" id="pgr:PGTG_10819"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10819"/>
    <dbReference type="HOGENOM" id="CLU_159482_0_0_1"/>
    <dbReference type="InParanoid" id="E3KK35"/>
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    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
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        <source>
            <dbReference type="EMBL" id="EFP84660.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="90" mass="9506" checksum="671CA3866BE3E87D" modified="2012-04-18" version="2">MGQTNVSFCHQINSGKGIPIIMPNSTQGTYGAVQPATPLSLTPTKCSNFKCTKIPIFHPPVMSSCKTIVQTLLNNSTGTFIAPPSKPSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3KKB4</accession>
    <name>E3KKB4_PUCGT</name>
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        <recommendedName>
            <fullName evidence="10">FFD box profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_10898</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329158.1">
        <property type="nucleotide sequence ID" value="XM_003329110.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KKB4"/>
    <dbReference type="STRING" id="418459.E3KKB4"/>
    <dbReference type="GeneID" id="10526707"/>
    <dbReference type="KEGG" id="pgr:PGTG_10898"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10898"/>
    <dbReference type="eggNOG" id="KOG1073">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019221_4_0_1"/>
    <dbReference type="InParanoid" id="E3KKB4"/>
    <dbReference type="OMA" id="WYPPPGH"/>
    <dbReference type="OrthoDB" id="21539at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033962">
        <property type="term" value="P:P-body assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034063">
        <property type="term" value="P:stress granule assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01736">
        <property type="entry name" value="LSm14_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025762">
        <property type="entry name" value="DFDF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019050">
        <property type="entry name" value="FDF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025761">
        <property type="entry name" value="FFD_box"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025609">
        <property type="entry name" value="Lsm14-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
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        <property type="entry name" value="TFG_box"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13586">
        <property type="entry name" value="SCD6 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13586:SF0">
        <property type="entry name" value="TRAILER HITCH, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09532">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="LSM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01199">
        <property type="entry name" value="FDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01271">
        <property type="entry name" value="LSM14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51512">
        <property type="entry name" value="DFDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51513">
        <property type="entry name" value="FFD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51536">
        <property type="entry name" value="TFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sm" evidence="7">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="DFDF" evidence="4">
        <location>
            <begin position="383"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="FFD box profile" evidence="5">
        <location>
            <begin position="461"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="domain" description="TFG box profile" evidence="6">
        <location>
            <begin position="518"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="154"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="576"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="FFD box" evidence="1">
        <location>
            <begin position="461"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="TFG box" evidence="2">
        <location>
            <begin position="518"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="3">
        <location>
            <begin position="154"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="177"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="217"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="277"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="291"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="335"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="355"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="397"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="415"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="463"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
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            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
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            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="586"/>
            <end position="599"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51512"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51536"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP84739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="629" mass="65192" checksum="9A86F2A5942D41A2" modified="2011-01-11" version="1">MAQQYIGSRISLISKSDIRYRGILHSIDPAASTVSLEQVRSMGTEGRKSNPSEEIAATDQLYEFIVFRAADVKDLSIEAPAEAKPEPPKPSVPDDPAIMSTTAPPRAPPNPYMQQNIMGPPQGMYNNTMPPMMYPPGNVPPQWPPYGMPPPGQIHPMMGFPPQPMPPPNVPLAQQPNHQSNQTNVPPSTKPNPAASAGKAIPPHKVDATGSALNQKASPLASLSPNKPSSSLASNPLPPSSAQSSGPGGPVLPSSQPARGPAGVNGAAGSGKSVFGTTTAMDMMASIAAMSPQQGSPANQQASSTSQKFPTNSANIHPNQNGTQGANYAQSGALQPSTSQSQQQHQMRHPLPQVPNNNKAKAAPKAHGASQPNNSNRPNHHHQSSSTPIVPAQDFDFASANAKFNKAAPPSNSIKAEDGSSQKEDVEPGLGAEGPVDGAKKIGGESKEEDDFVIPEAPPGTYYDKKKSFFDDISSEVKERSALSSQDGPSKPNPGGPNGANAGRGARGGAGGRGGRGGKGWDRNAERQKNLDTFGEVALNPNLGALGSGYNGYGRGGAHRMGQIGGAVNGGYRGMPGRGRGGPGRGNHHHHHNHHHHNHPAAAAARGGFPVNLRAIHLAAGGGPSPVNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNC9</accession>
    <name>E3KNC9_PUCGT</name>
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        <recommendedName>
            <fullName evidence="3">mannan endo-1,4-beta-mannosidase</fullName>
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        </recommendedName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Random hydrolysis of (1-&gt;4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.</text>
            <dbReference type="EC" id="3.2.1.78"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.78" evidence="3"/>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EFP85765.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330184.2">
        <property type="nucleotide sequence ID" value="XM_003330136.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNC9"/>
    <dbReference type="STRING" id="418459.E3KNC9"/>
    <dbReference type="GeneID" id="10544338"/>
    <dbReference type="KEGG" id="pgr:PGTG_11094"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11094"/>
    <dbReference type="eggNOG" id="ENOG502QQFD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031603_0_0_1"/>
    <dbReference type="InParanoid" id="E3KNC9"/>
    <dbReference type="OrthoDB" id="2498309at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016985">
        <property type="term" value="F:mannan endo-1,4-beta-mannosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000299">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045053">
        <property type="entry name" value="MAN-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31451">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31451:SF40">
        <property type="entry name" value="GLYCOSIDE HYDROLASE FAMILY 5 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26410">
        <property type="entry name" value="GH5_mannosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173552" description="mannan endo-1,4-beta-mannosidase" evidence="6">
        <location>
            <begin position="28"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="7">
        <location>
            <begin position="34"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF26410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP85765.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="419" mass="47903" checksum="609CCA5391773AD3" modified="2012-04-18" version="2">MVFPVRRVAFTVLLTWILPICKLVVVANNVTSKRDFITVENGSFFKSCSRHYLVSMNYWSAMNLAADETAGGNLTRFKIKVKQLAEMGVNNVRIMAALEAAGHPPYRIYPALMYAPGRYNEEMFVGLDRALVEFSRYNISVVMTLNNFWDWSGGYAQYVSWATNNSQVPPASPWDPSLNPPYGNWSTSGIYGKYDPATGNWDGFVGYAGRFYNDSSITNLTQTWFKNHIHTVVNRQNTISGVVYKEDPTIMTWELTNEPQDPPVKWSVFISYTFISLKLDSKMLQLMLVRVEDTSTYIKSIAPNHLVTVGFEGKNGEWWFKRVHAPRSVDYTCGHLWVQNWGVYDPLDPTEKSLKAAKIFANGYLKDLSKWTLDLKKPLVLEEFGMARDNWLNVKKGAPKTHYLYERMKDGKELGKFSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNV5</accession>
    <name>E3KNV5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_11736</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TUBGCP family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178297">
        <property type="protein sequence ID" value="EFP85980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330399.1">
        <property type="nucleotide sequence ID" value="XM_003330351.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNV5"/>
    <dbReference type="FunCoup" id="E3KNV5">
        <property type="interactions" value="635"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KNV5"/>
    <dbReference type="GeneID" id="10539979"/>
    <dbReference type="KEGG" id="pgr:PGTG_11736"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11736"/>
    <dbReference type="eggNOG" id="KOG2000">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003736_2_0_1"/>
    <dbReference type="InParanoid" id="E3KNV5"/>
    <dbReference type="OMA" id="MRMMSVC"/>
    <dbReference type="OrthoDB" id="5860513at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000930">
        <property type="term" value="C:gamma-tubulin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000922">
        <property type="term" value="C:spindle pole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043015">
        <property type="term" value="F:gamma-tubulin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051225">
        <property type="term" value="P:spindle assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1900">
        <property type="entry name" value="Gamma-tubulin complex, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007259">
        <property type="entry name" value="GCP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040457">
        <property type="entry name" value="GCP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042241">
        <property type="entry name" value="GCP_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041470">
        <property type="entry name" value="GCP_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302">
        <property type="entry name" value="GAMMA TUBULIN COMPLEX PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19302:SF14">
        <property type="entry name" value="GAMMA-TUBULIN COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04130">
        <property type="entry name" value="GCP_C_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17681">
        <property type="entry name" value="GCP_N_terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma tubulin complex component protein N-terminal" evidence="9">
        <location>
            <begin position="162"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Gamma tubulin complex component C-terminal" evidence="8">
        <location>
            <begin position="488"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="742"/>
            <end position="787"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="843"/>
            <end position="880"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="9"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="755"/>
            <end position="766"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="856"/>
            <end position="876"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP85980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="897" mass="102601" checksum="A37F5EBD39DFB1E2" modified="2011-01-11" version="1">MENNNNKQLKHLKELIRRITNNNQQQQQQYSVYQQKLNNNNPTTTTTTTNQSLNTIAEQIKQHLQLDPNQPIQAIQHFTSLLTRLSPLSHPKEKTATLKFLLALAPKQQQQQQKQQQQEPPTIIERLPSTETTQPQPSAAQRLKQYRKQHAYPAHLPEHLLLRDMIYLLQGIDGRYIYFKSSNSEDESWEEGGIGFKEPTHHQLITLPQRDLILKLTELGWLYKKIERAIDQPEANIGTVQQAFAFALKEELGTYYRAIAIIQSHLSSDPSTTLSSKALLLHLTPTVLRLRMSAALISATKGIRGGQMISVLHSYTDHGDPVVHLFTSNLLEKVSVPWFKTLVSWMWDGELVDPIEEFFIILNPAFDRKTNNNHHLHNNLDQLDHLHQLPGDGSNVWQKKFSFRKEMIPSFISEGFARKIFSTGKSLNFIRHSCGDYEWHQTRSNIYQASSKASLHYKDITGLQQTIDSTYSIATRQLFNIFFVKLKLLDHFKAVKDYLLLGRGDFISLLIETLGPSLNKPANTLYRHNLTATLESAIRATSDERLLLNRLDVRMLEFSPAESGWDVFMLEYKTEKPLDVVLSASAMEKYMRMFRLLWKMKRLEYSLDRAWKVVILGVNRALKHLHCLRGDFHKARLVISEMIHFIRQLQSYCHLEVIDCGWQEFEKKLTTEGGDLDSLIEGHAAYLDRLVSKGLLLSTRVGKENTCLLLAEACFKVILEFKDSIDNLYAYGLSKSARLATDQQGTKSKRPKYEDSEEEGGGYDSDEERRAGEGDEEEQSSADRSSEIQLESIRIQLSNHSKRFTELVLDLISSLSTQQDSDMKFLAVRLNFSLFYMRTSKSSASSSLAVPPHPSGSKTSANPIPPTSRSSVNYDSRASAPLHVHRPLSYQIPKRTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPD2</accession>
    <name>E3KPD2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_12113</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178298">
        <property type="protein sequence ID" value="EFP86157.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330576.2">
        <property type="nucleotide sequence ID" value="XM_003330528.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KPD2"/>
    <dbReference type="GeneID" id="10540166"/>
    <dbReference type="KEGG" id="pgr:PGTG_12113"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12113"/>
    <dbReference type="HOGENOM" id="CLU_415680_0_0_1"/>
    <dbReference type="InParanoid" id="E3KPD2"/>
    <dbReference type="OrthoDB" id="2497191at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP86157.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="693" mass="79973" checksum="BDC1D1BB1E8AA9CB" modified="2012-04-18" version="2">MFVSSSLYTSTGGVMGSSSGPNTSYLPTYLGLCIPGMYASRPLWFSSVHPGGAYDQSVAIERGHRSSDLLEQISVKEGSIRGGLDRPSSQKNQIPERRKHAIRKSKTLLSDYKTEFPVYVVGPSTTFGRLIDKLAEEHSLTPQMEKITALNEDLMELLPYESHINQMFDFIKWNTDQKQNPNIYTASSIIQEFQRKVRIFAKLRLLKLGDYDSNESYWKIGQLLLPALQFPSTQRSRLEAEKQLQAVAGSVLDGVKELMTKYNTEAELMKWEYHELDWINTWRYTFKVVDLLLRNGFINQEKFLIFSQDQELAKLVVVSFINLIEKRPSWLQLKAKTDFGNLDNTSKEIDDDMRRVIEEPYSKRAMRHWKNSGSTDFKSFEESLVSGFSRKERSLRNGIERGSSDEYSAFKPQQHYIMKTEKSLSPGYRSHDNGVWNYDRLSQLTRVFRELMDRLTGDTSWSREIRSRIIGNWVVNSDKVMALHKNLVELSHYQVPLVGTFQDVKRTRNISQFPNIYMASNLIEECQKKIEILTKLRLLELGQHDSDVSYHRLIKSLLPTFKFPVDLSGESFQLEVKNNLEDVSTKILGNVKTLLTTDGLESELSKWTRSQRNWHLTCGFAFAVVDSLSENDFINPKKIKRFLQEKAIAKVVLDYSKSIWRQDTGARDYSQQGPWSELNTSFKSEQNCIFLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPQ2</accession>
    <name>E3KPQ2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_12243</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178299">
        <property type="protein sequence ID" value="EFP86287.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330706.2">
        <property type="nucleotide sequence ID" value="XM_003330658.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KPQ2"/>
    <dbReference type="GeneID" id="10540300"/>
    <dbReference type="KEGG" id="pgr:PGTG_12243"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12243"/>
    <dbReference type="HOGENOM" id="CLU_2147092_0_0_1"/>
    <dbReference type="InParanoid" id="E3KPQ2"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP86287.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="112" mass="11809" checksum="53927ABDF516507B" modified="2012-04-18" version="2">MGHSLGPVNSPKPATATWYQLAGSGGGNLGELIPAVKDNWVKVLEGSGPVLGELWYLNRFAGEASTRRAGHGVLIKYIPLVASIDVIHLSMPTMPPPSGTGVIQAIFNVYVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQ50</accession>
    <name>E3KQ50_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12381</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178300">
        <property type="protein sequence ID" value="EFP86425.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330844.2">
        <property type="nucleotide sequence ID" value="XM_003330796.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQ50"/>
    <dbReference type="GeneID" id="10540440"/>
    <dbReference type="KEGG" id="pgr:PGTG_12381"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12381"/>
    <dbReference type="HOGENOM" id="CLU_2428110_0_0_1"/>
    <dbReference type="InParanoid" id="E3KQ50"/>
    <dbReference type="OrthoDB" id="10514395at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="76"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86425.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="91" mass="9802" checksum="1BC46914DA0C665A" modified="2012-04-18" version="2">MTKATPSAPLTLGCDEFIWRVHAFLDSQGLCHFCKRHCGNAAGACPGPIDRSYLNIPNEFQTSTKPLNYVAPRAWSKPTTSPGQSSQPPAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3KSA5</accession>
    <name>E3KSA5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tyrosinase copper-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_13399</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EFP87180.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331599.2">
        <property type="nucleotide sequence ID" value="XM_003331551.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KSA5"/>
    <dbReference type="STRING" id="418459.E3KSA5"/>
    <dbReference type="GeneID" id="10537410"/>
    <dbReference type="KEGG" id="pgr:PGTG_13399"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13399"/>
    <dbReference type="eggNOG" id="ENOG502S31Y">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035914_2_0_1"/>
    <dbReference type="InParanoid" id="E3KSA5"/>
    <dbReference type="OrthoDB" id="6132182at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1280.10">
        <property type="entry name" value="Di-copper center containing domain from catechol oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008922">
        <property type="entry name" value="Di-copper_centre_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050316">
        <property type="entry name" value="Tyrosinase/Hemocyanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002227">
        <property type="entry name" value="Tyrosinase_Cu-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11474">
        <property type="entry name" value="TYROSINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11474:SF126">
        <property type="entry name" value="TYROSINASE-LIKE PROTEIN TYR-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00264">
        <property type="entry name" value="Tyrosinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00092">
        <property type="entry name" value="TYROSINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48056">
        <property type="entry name" value="Di-copper centre-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0186">Copper</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosinase copper-binding" evidence="3">
        <location>
            <begin position="127"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP87180.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="367" mass="41631" checksum="FCE9E5A19742683C" modified="2012-04-18" version="2">MEGMTTPPHLRTFAVTGDGVCITQSCHLQGLSENREDGCCNSFSVPKNVHPHEAQSLALSQWQFASWFHKSLFIVYLVRETSSALEIVPLAEILPTNLSASEKRAYQASVSCLLKAPSQMRYPGAVSRYDDLVYVHQRQSDYPNGRDEWHVTGQFLAVHRMYCYIFELMLRDECNYRGRMPYWDEQRDAGNFRNSEFLRDFGGPGDERGMVTQGPFANFEINLGPGFQNVRRRLHRQINETASAMAGPQYYQKLMAQPTFASFLNEIRQHSHIAGHNGVGGELGDVQTAAVDSIFFNHHLYIDWLWASWQEADPQARIFDLRGAGYETQANPTQETNLLTTLKFLGLAPDVPLYGSLDIQVLSPFEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KSK5</accession>
    <name>E3KSK5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_12875</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178305">
        <property type="protein sequence ID" value="EFP87291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331710.1">
        <property type="nucleotide sequence ID" value="XM_003331662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KSK5"/>
    <dbReference type="GeneID" id="10537690"/>
    <dbReference type="KEGG" id="pgr:PGTG_12875"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12875"/>
    <dbReference type="HOGENOM" id="CLU_2159671_0_0_1"/>
    <dbReference type="InParanoid" id="E3KSK5"/>
    <dbReference type="OrthoDB" id="2510290at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP87291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="111" mass="12769" checksum="D0B0C2DBB17874E2" modified="2011-01-11" version="1">MKPRHTLLSPDPLKSITVATLNSCKQNDKDDHLCMPNSCYTDKGGARWEAVFFIKCYKDQIAGAQIKRDTLKPAQYFRRESFAEVQEVGTQIWFTCPYSYDHNAQIMMEGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KSM4</accession>
    <name>E3KSM4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12894</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178305">
        <property type="protein sequence ID" value="EFP87310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331729.1">
        <property type="nucleotide sequence ID" value="XM_003331681.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KSM4"/>
    <dbReference type="GeneID" id="10537710"/>
    <dbReference type="KEGG" id="pgr:PGTG_12894"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12894"/>
    <dbReference type="HOGENOM" id="CLU_2387251_0_0_1"/>
    <dbReference type="InParanoid" id="E3KSM4"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="94" mass="10136" checksum="3BDAA0B25D78355D" modified="2011-01-11" version="1">MNKPGRPGPTKVSGPVVIVWSCRDGTGDVYPAGLSKIPLPTSNGGLHGDPTSRNHQPLVEPLSSTTTKERDRLFISLGKKCLMFLVSKYEDDGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3KU95</accession>
    <name>E3KU95_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF659 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14585</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <dbReference type="eggNOG" id="ENOG502SPG0">
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052717">
        <property type="entry name" value="Vacuolar_transposase_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46169">
        <property type="entry name" value="DNA REPLICATION-RELATED ELEMENT FACTOR, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46169:SF15">
        <property type="entry name" value="INNER CENTROMERE PROTEIN A-LIKE ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
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            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="382"/>
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        </location>
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            <begin position="382"/>
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        </location>
    </feature>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP87870.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="632" mass="71322" checksum="03D555454591BFAA" modified="2012-04-18" version="2">MSRKKSNPGPVLIDDDSEVKPSPSVDATTPGSLSKLSKAILTDEQELKKAQRVHANQQSACYAYFNPPILSDAKDKNGRLMIAYPCKMCGGKTNRPIYESSPTNLAKHVASCTKKQREANSNQKLADMGVSGTGDIDPREVPQLCAIWCAQSAQPFASLGETSHLGIVHPTVIKNLPKRRTVSNDIAKLYTAVQESLIESFKNTKGAMYLGLDAWQSPNGFDVLGTVIYRLVKEEEGGFHLEAMPLDFVRLQKSHTGLYLAETVQLIVEKFGLQDKIYGIVTDNASNNRSMIEELQTFRWPHFKGEATWVVLRPFGSHKRTKTVVSDNEESDEEETEDQDNQIRLATHDSADDGDDDEDDAGSIIDDSALAAELIDDDEVELEDDDVNELSDEDEDDQYTSNSCRKTLAKFRAIARKLNKSPNSKAHFVEICQDLECVRPHNIARDVRTRWNSTLTQLSSIVRCSSAILEWQKDRRHGPSQEYYINEDDIHLARDLIEVLEPFYEITLQVSMHGAARISDIVVFIDQITSHLSTIICDKKDKYPPALRNACRAGLQLTNKYYTLTDCSPLYRVAMVLHPSFKDEYFKLAKWKPEWIQEAIRLTREMWENHYKPSPQATNSQPANQAMQDILF</sequence>
</entry>
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    <name>E3KV07_PUCGT</name>
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            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12554</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
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    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178311">
        <property type="protein sequence ID" value="EFP88107.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="KEGG" id="pgr:PGTG_12554"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12554"/>
    <dbReference type="HOGENOM" id="CLU_136933_1_0_1"/>
    <dbReference type="InParanoid" id="E3KV07"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="39"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88107.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="130" mass="14229" checksum="8ADCDC707EEDE8E9" modified="2012-04-18" version="2">MTTRSNTNPDKLFPLTDPDAIIRSGNAEQRGLKQLKSNPTAPLPLLSKTTPPTAMSGEIAPAHERPGRLAQQTPQTCRQLKTGSSLSSRFNMCSSLKHRPTIDKLQRIAEKLRRIAALTDVELLFGGNRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVI3</accession>
    <name>E3KVI3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14407</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
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            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178313">
        <property type="protein sequence ID" value="EFP88323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332742.1">
        <property type="nucleotide sequence ID" value="XM_003332694.1"/>
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    <dbReference type="AlphaFoldDB" id="E3KVI3"/>
    <dbReference type="STRING" id="418459.E3KVI3"/>
    <dbReference type="GeneID" id="10535715"/>
    <dbReference type="KEGG" id="pgr:PGTG_14407"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14407"/>
    <dbReference type="HOGENOM" id="CLU_033090_3_0_1"/>
    <dbReference type="InParanoid" id="E3KVI3"/>
    <dbReference type="OrthoDB" id="2506958at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="425" mass="48637" checksum="879061D7DA21E46E" modified="2011-01-11" version="1">MTDSTNKPSEEPKKLSKEQQLRQQGDLVIQGYKDLADKTSSFLHKHNELLYLTRSRPASFQNAVSIDQAHLKKGLLVQLQSSLVPQLQQQIFTTLQLTNPYDLTKDPGLQFKCILEVQAELNPILDQIFSATDVLCPGPLPSPSIRTNDQYLKELKPFRIRALYDHLTGCFLNQIFKVCNDSYDLIQQLKLSTKKYEGEVDLASARYYLNAEGDYCLQLIERAINCLEGSEFDVVQVDWRYNQSVINGHLRELLRRINPTTPLDEQNSMPGPQTLSEPVVNLTKSVLPVIKLCRLFFNKLSERGMNRKRLPLFTTMCSYQLNLISRLAGDIAGKLDRILSILRAADTTLNENIIRSLTKTVKALETHFETPFLLILLHFLPIIPDTVGNPVQNYFKEWFAIWNTQFTLAIKNLLNVIESYKGDFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVY6</accession>
    <name>E3KVY6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178314">
        <property type="protein sequence ID" value="EFP88461.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332880.2">
        <property type="nucleotide sequence ID" value="XM_003332832.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KVY6"/>
    <dbReference type="GeneID" id="10540764"/>
    <dbReference type="KEGG" id="pgr:PGTG_14039"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14039"/>
    <dbReference type="HOGENOM" id="CLU_2016327_0_0_1"/>
    <dbReference type="InParanoid" id="E3KVY6"/>
    <dbReference type="OrthoDB" id="2508889at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173951" evidence="1">
        <location>
            <begin position="27"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88461.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="125" mass="13902" checksum="1EAD325DCBEBBFD5" modified="2012-04-18" version="2">MPGVRTIARSAFVCALLLKSFLVAPGIPGPPYDPNLYTDRCQRCWVEAGSPPEFINTMYKTNTFNSFPCSAFSHVYTCLERVGLQGYYCVRCRHFLNVSLGACSIHGQFTQCTPDPRRPQQGGTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>E3KW68</accession>
    <name>E3KW68_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">ubiquitinyl hydrolase 1</fullName>
            <ecNumber evidence="2">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_14121</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="2"/>
    <dbReference type="EMBL" id="DS178314">
        <property type="protein sequence ID" value="EFP88543.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332962.2">
        <property type="nucleotide sequence ID" value="XM_003332914.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KW68"/>
    <dbReference type="FunCoup" id="E3KW68">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KW68"/>
    <dbReference type="GeneID" id="10541009"/>
    <dbReference type="KEGG" id="pgr:PGTG_14121"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14121"/>
    <dbReference type="eggNOG" id="KOG1863">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_268739_0_0_1"/>
    <dbReference type="InParanoid" id="E3KW68"/>
    <dbReference type="OrthoDB" id="2420415at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02257">
        <property type="entry name" value="Peptidase_C19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000122">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044635">
        <property type="entry name" value="UBP14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025305">
        <property type="entry name" value="UCH_repeat_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43982">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43982:SF6">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13446">
        <property type="entry name" value="RPT"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="8">
        <location>
            <begin position="392"/>
            <end position="1095"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="22"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="495"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="930"/>
            <end position="1029"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1077"/>
            <end position="1151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1190"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="44"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="501"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="552"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="604"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="627"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="962"/>
            <end position="977"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1013"/>
            <end position="1024"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="1083"/>
            <end position="1098"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1190"/>
            <end position="1204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP88543.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1220" mass="134083" checksum="B2C53B24EE990541" modified="2012-04-18" version="2">MPWDPVSEKIWASLGVIRKEVSESLSDDSSPPPPVASATDHPKTSPSNPASSSVPSAQGPTPLLVLPSLNVKTPEGRKNRAIWCRALLEISVFLKWYKLQHPHIEESIKSNYIVPFNLVSAYPKIVQMLGGNSIPVFPTKSTWQTPNGKPVAYAAYAKLGIVPSVPDGVIISGYEVQVRTFPHLRYEFYEALREIQMIRESQSLAVFLGIEASRGVVGLTEARLAWNLICGDGKDPSFDNMDLTREDFMMNAYNAKEAAETNPAEKTKLRNALRTLVEANPTNELLRVISDTANSAAEEKANPMDLNKAYATLGDIIPEVDDEMLWMAYTIGVSDQPGQKDVLREALQVIARHRKSQLIESRLVAEIKGGNAESAMEIVPYSPPPVSYDLPAGLNNIGNTCYLNSLLQYFFSVRELREILLRFPEFEQDIDEHQEMKEKKRVGGRIVSASEILRSKQFASQLQGLFREMISTPLSAVTPERELAFLALVLSKDESPMSVPTTTTNANHNSSASTDATLVDDQPVILGPTFNATPSPPANVASAGSSTVLGKRKSESSGSDDGHSTSRNLMDVDSVPPATADKELIGPTKDLAMAGSSPILPTSEPDPPPPPKATAPLIIDLTDDSNNPPPPPPPPLPPRPNRSKSVTAIESGSHMMFGRQNDVSECMDNCLFQIQAALDQEKIRASGEFDGDGNLVKSLFYGKTRQSLMFENPKGKVSIKEEQFAYLLVDVAEEGRDLYDGLDKVFDESEVELENGKACRRVGLVELPPILQIQLQRVQFDRRTHTVFKSNAHLKFGQRLRMDRYLEPDPDDQVGQEKRTKTIGLRKEIECLRGRLGELTTNVSGDKRTASTVLRDLHGILSSSADPSSCPSRAGSSPGTRLDFFHGLLGPEELDYLDAEATAIDAEIAGNKVAIKRLKAHVEEMWKVDETNENAPASRSEEFSPPSNTTAHLPPSLDHNLPTKSSTTTTTTPTSTPVVPMNVEPSQSHLPPASGNLNKDKDRRLEQDDDDEPVIKDDRIDKHPSSSSSQTAEYILVGLFMHRGTAQGGHYWAIQRQLPDRLDRWLKYNDSQVSEIDPKVEVFNDDDEHADDDEEEGGETVGEQMVVEGPKARDEDDQTKTPPSTDLQLPHQIKPSSSGFPASKNRQKRTADNANPYWLTYVRKDQLNRFQMVHRNLHAFDPPVVISSSATPPVLSSCSSSSSAPLPPATLVPADPIVLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3KWC0</accession>
    <name>E3KWC0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein yippee-like</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_14800</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the yippee family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178315">
        <property type="protein sequence ID" value="EFP88595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333014.1">
        <property type="nucleotide sequence ID" value="XM_003332966.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KWC0"/>
    <dbReference type="FunCoup" id="E3KWC0">
        <property type="interactions" value="345"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KWC0"/>
    <dbReference type="GeneID" id="10541222"/>
    <dbReference type="KEGG" id="pgr:PGTG_14800"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14800"/>
    <dbReference type="eggNOG" id="KOG3399">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043857_3_2_1"/>
    <dbReference type="InParanoid" id="E3KWC0"/>
    <dbReference type="OMA" id="SSRIYGC"/>
    <dbReference type="OrthoDB" id="6407410at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000151">
        <property type="term" value="C:ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034751">
        <property type="entry name" value="Yippee"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004910">
        <property type="entry name" value="Yippee/Mis18/Cereblon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039058">
        <property type="entry name" value="Yippee_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13848">
        <property type="entry name" value="PROTEIN YIPPEE-LIKE CG15309-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03226">
        <property type="entry name" value="Yippee-Mis18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51792">
        <property type="entry name" value="YIPPEE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Yippee" evidence="5">
        <location>
            <begin position="13"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU110713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP88595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="110" mass="12461" checksum="AE5CF260D8DFECFC" modified="2011-01-11" version="1">MGLKHREFLSGSRIFGCSTCRTHLATIDNMISRQFNGQHGRAYLFTNAVNISLGEAEDRPMTTGLHTVRDIFCAKCGTTMGWKYERAYDQNQKYKEGKVILEKALLVDVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KWP0</accession>
    <name>E3KWP0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_14673</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178316">
        <property type="protein sequence ID" value="EFP88707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333126.1">
        <property type="nucleotide sequence ID" value="XM_003333078.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KWP0"/>
    <dbReference type="GeneID" id="10541497"/>
    <dbReference type="KEGG" id="pgr:PGTG_14673"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14673"/>
    <dbReference type="HOGENOM" id="CLU_1435079_0_0_1"/>
    <dbReference type="InParanoid" id="E3KWP0"/>
    <dbReference type="OrthoDB" id="10271144at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173794" evidence="2">
        <location>
            <begin position="20"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="166"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP88707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="189" mass="20646" checksum="2252984245EA8914" modified="2011-01-11" version="1">MLRLPIIILACFTPNYILAAPTELANLAPPPTVFGNAPGPFMETALYQDNLLRNRKLLLEAVSVPSPMSAPIPKQSMDRNEDVSSPTPVQAELIRWFMKTRYGGAFTLNDDFTFHERHKKCCEVEYCCGKTKIYNQAGALIKKTGCCHSPDHYSSRASFNCGECSVIFGSIAGGVLIAALAVCLTAKNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KX79</accession>
    <name>E3KX79_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_14188</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178317">
        <property type="protein sequence ID" value="EFP88849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333268.1">
        <property type="nucleotide sequence ID" value="XM_003333220.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KX79"/>
    <dbReference type="GeneID" id="10541964"/>
    <dbReference type="KEGG" id="pgr:PGTG_14188"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14188"/>
    <dbReference type="HOGENOM" id="CLU_1267448_0_0_1"/>
    <dbReference type="InParanoid" id="E3KX79"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP88849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="218" mass="25694" checksum="8D9E2B5F1FB60A71" modified="2011-01-11" version="1">MKFLMKISTKAPWDFESLVTSRKVKVSLDRLIPLVLKPFKEKFQEAPLRNHYLSIHPRVSIAVYFLKDEPNVGWIRVIKKPQIQILTKKKATNLLTKLAMAVTYIHVELQRSTSRQGKDFIQKRKAIFQWLITVIFEPKQGFPIYGKLKINPGLAPWEEERYRNTVIFTPVQLRLIQYFSEPLTSLTLRETAAFIITSWYHDHDDTEFCSWAKLPFQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3KXX5</accession>
    <name>E3KXX5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tc1-like transposase DDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_14902</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178319">
        <property type="protein sequence ID" value="EFP89061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333480.1">
        <property type="nucleotide sequence ID" value="XM_003333432.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KXX5"/>
    <dbReference type="GeneID" id="10546529"/>
    <dbReference type="KEGG" id="pgr:PGTG_14902"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14902"/>
    <dbReference type="HOGENOM" id="CLU_046169_0_0_1"/>
    <dbReference type="InParanoid" id="E3KXX5"/>
    <dbReference type="OrthoDB" id="2498125at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP89061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="403" mass="44825" checksum="63933C6DFD10E2F8" modified="2011-01-11" version="1">MLQPPARVWAQAPVPRVLAPRGPGPAPRGPDLAGRAGVGPWHWPKSGLAAPKLWAHQITKVQHRLPPPPSMSKSAVMDPTDLEIQANKERSREAIKKAKANVDETRTYKNDRLTHKERKPNAIIKPEVRDLIRKNVNGKGMKIGDAIKKFNVSRRQIQRIKGEDPNLIRTHKKRPGKFTDDMKTKLLMQLDQKSTTTLVKMVAFIKDRFDVKVSTQAISNLIHDMDISWKQVTNIHAAWNKPNLIEQRANFVNRRGLDLGQRVVFVDEAGFDLHSSRASGYAPSGQPAVLSLVPKAKQITLIGALLVEGFDYYELLNADNTKAKGVGADEFCLFLGSLGARLPKEAIIIIGGHHITRRLQIDQGGIFTTLLSVSQSDRIQLPFDQIICLIKGATQPNGVSRRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KYN0</accession>
    <name>E3KYN0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15268</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178322">
        <property type="protein sequence ID" value="EFP89426.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333845.2">
        <property type="nucleotide sequence ID" value="XM_003333797.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KYN0"/>
    <dbReference type="GeneID" id="10547313"/>
    <dbReference type="KEGG" id="pgr:PGTG_15268"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15268"/>
    <dbReference type="HOGENOM" id="CLU_096898_0_0_1"/>
    <dbReference type="InParanoid" id="E3KYN0"/>
    <dbReference type="OrthoDB" id="10496600at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="174"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89426.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="251" mass="28008" checksum="8AE88A8B67ED3DBB" modified="2012-04-18" version="2">MALASDSTQFLLHRVDVAADGLPAYHPNATKPIFRKFKEKGLSMAIEILETHGIRQFVLKLYIDTLLGQCSKATLPIGHVNLTRTLETLGHEDYGQKVIRTAYKEHPKGPLFLITFGTAKCNGSLIVSRWQGILKNVEDLDRLKRTLDPILGYGMKLHPPANQLINSSNQKSRAPSSSATATPIVHTSPTDPSSTLPHKIEILRKSQSMSSLDHLQPHEFHELMKRVNRSHSFDKAIEIVKAHKGHHVIQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3L066</accession>
    <name>E3L066_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6589 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_16241</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178327">
        <property type="protein sequence ID" value="EFP89953.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334372.2">
        <property type="nucleotide sequence ID" value="XM_003334324.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L066"/>
    <dbReference type="GeneID" id="10545768"/>
    <dbReference type="KEGG" id="pgr:PGTG_16241"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16241"/>
    <dbReference type="eggNOG" id="ENOG502SCRC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009176_0_0_1"/>
    <dbReference type="InParanoid" id="E3L066"/>
    <dbReference type="OrthoDB" id="2496743at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046496">
        <property type="entry name" value="DUF6589"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20231">
        <property type="entry name" value="DUF6589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6589" evidence="2">
        <location>
            <begin position="347"/>
            <end position="773"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="862"/>
            <end position="887"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP89953.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="887" mass="101515" checksum="A9AAA0915E024B26" modified="2012-04-18" version="2">MHKSDALVDKVIYICDLLQELDMTPKSFIMGFLQLDNSNLKLRRGYWGIWRGWPSTFTVVGAIRDELLRSAPGTLEWSNYIRDQAIWILRSQNPRSGIHPRGSYISSSAITPEIFNEHSKEEHRRRLTAHEMPFLYQMVLGMLSGPSNVNDTDNKDDQKVTSPVEKAALAPIDALEEELMEREGFRYTSGKTGVDHSLSRHERISYTVCSMLSFAKNRRHNPFQLENSIRFLACGISERVNEYLHHLGLTSSRQTAIDSLKTLSSYAQGQLTRVMALDVNPAFGPFICIDNLDMEERVHMASVGHRSMMFHGTWGYIHTPHKALLDSLDWSEINLNAFNQALQTVRTMSITPRDFLPDRQTEDHYCLVWKSQLASVMMKYIAEPATKAGAYPNEPPELEVLSPQAPNFHMLKLMEESDNSAEGIGQVIESVQRQTGLTPEEFAGRLQPMEGDLGTCQNFNTMRTLRNPNKRTNENMNNITIQLGASHTLWNIGQTIFTTHFGDVNSSENMGAWRTLNALGTPPSKVLQKKDFTGMIQHLERVHEATLFHCLRVVMKIDRQPITDDRPKIDTPKWNQIIDDCYQRYCSPQARRTAYEECSIPKLPKKKKLSEAEVELLNQRHRRSKLSNLLGRLHDFSTVVEANRAMKHGDIGRLINVWRMWSVMAQSLPGLTHYATYLPRLVLLLTKVLSPSLSKYFRHSMLVSPSGRPCHFVAKDFFLENHNYWLKFFFNRGGIGTQVDRLKNLFSMNIPLLRSLFLSLQVDSGKHRVYQSHKVTLSKSSLKSFQQMAHNSDILNQHNRTQYYRDLTIDSTYVEGLVSFQDIISHKDNDLSRLRSHIPSHYQHTDDEHVAEARVMLNSEDDALGSIDPKDTISNPEEVDPPSSDCM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3L198</accession>
    <name>E3L198_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16319</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178331">
        <property type="protein sequence ID" value="EFP90293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334712.1">
        <property type="nucleotide sequence ID" value="XM_003334664.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L198"/>
    <dbReference type="GeneID" id="10530187"/>
    <dbReference type="KEGG" id="pgr:PGTG_16319"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16319"/>
    <dbReference type="HOGENOM" id="CLU_078036_0_0_1"/>
    <dbReference type="InParanoid" id="E3L198"/>
    <dbReference type="OMA" id="ISEGWDD"/>
    <dbReference type="OrthoDB" id="2499072at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033018">
        <property type="term" value="C:sarcoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051279">
        <property type="term" value="P:regulation of release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10033">
        <property type="entry name" value="CALSEQUESTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10033:SF0">
        <property type="entry name" value="CALSEQUESTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="51"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="310" mass="34749" checksum="7C19E2AB37C13B54" modified="2011-01-11" version="1">MWAKIGPKAPPSSTKLPSATPETFHRVKSKVGTGQSQASKTKSSRKRPRISTNSNSDSSSDSTSEDEPENPTSSETEQANPTSKNQHTSNRPPQDEEVGFDLDNFESHLESWSLSELRATLQKKKHGTSNRISPEVQERLELLQQNYMKSKLMLALIGNVAEKTVTKFLGENKPPRRKSGWNRFAAFSLESLKYPVPPKGASKGWEEQNIEIGKAWKLLDVDERAVFSARIFQHFSGIPCNFEDEPEDNEEESDLTTDEIELNKPLYNKLVNIEKVNIFAAKGSECQGENTSQTYKKALSATLKLNSEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>E3L1X8</accession>
    <name>E3L1X8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="7">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_16579</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="7"/>
    <dbReference type="EMBL" id="DS178334">
        <property type="protein sequence ID" value="EFP90553.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334972.2">
        <property type="nucleotide sequence ID" value="XM_003334924.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L1X8"/>
    <dbReference type="STRING" id="418459.E3L1X8"/>
    <dbReference type="GeneID" id="10530452"/>
    <dbReference type="KEGG" id="pgr:PGTG_16579"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16579"/>
    <dbReference type="HOGENOM" id="CLU_011478_1_1_1"/>
    <dbReference type="InParanoid" id="E3L1X8"/>
    <dbReference type="OrthoDB" id="2503069at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
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    </dbReference>
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        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="term" value="P:DNA replication"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
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        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
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        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="122"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="354"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="752"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="764"/>
            <end position="781"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP90553.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="781" mass="87619" checksum="A72F00AE1028C7CD" modified="2012-04-18" version="2">MSVDPTAEETVLPFEDESHRKDHPLQGSNSSLVGDPTEDPNLSSFGDITENPLPFEDISDCHPRVDTPLEKSSRAIKNKAGQLTLGKDIVNKSREKSIEMLKQRSKMVYGGQEPKQIQLDSVIDLIHRRDTFVLAGTGIGKSRIAEMYWDLFPKYKKPIVLVLNPLDSLGDNQVEEKKNAKISAINLTKMNLNEEVEEKILKGAYSLIYLSPEVLLNNPIFTRIFFNETFQNRVSLVVVDEAHMIYVWGLVASGKSKKIFSHGRHQDRGVFRPSYGDIASRLLGINAPLLLLSATCRPQAIKAILESLKITEEAITYRNAELSRPEICIVRVPMKSSLKSCDDLLKLYGPRCCIPDDEIIPTLIYSTTQNLTMQVSKVVHEGRRIMGGHNDANSTFARRFHACSGDMSKEETTTKFAAGDFPVIACTMALGLGQNWKRVRSVIHVGRGDPASICQMIGRCGRGGSNGLAILFVEKNRRSGKNTVKEFTNPTCQTDDERMDALAVTPVCLRICFSIDNKVGYVPLSCDDPNVARERDHEIAMDFPACLCSNCKPKEAMSVIDNFRKFRTSNFDHYMETGEDLPDNPLNTPYIRMPQQPTYRLGSTKEPLDPVLEELANQLILEFGRLFNKTFDAETAEVPADHMFEIQQARVFALAVKKGLPLTEITRVIGGEMIEGQMEHLKHTVVDIYCTGSTYQVFMEGKNKRKRKHTAVDTTTPKKIPTKAEIEKERKRSKWLADQVFLEQYKLENAIRIQKRNTVPKPTEANSNAMPNQAVSSSSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3L290</accession>
    <name>E3L290_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_16691</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178335">
        <property type="protein sequence ID" value="EFP90665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335084.1">
        <property type="nucleotide sequence ID" value="XM_003335036.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L290"/>
    <dbReference type="STRING" id="418459.E3L290"/>
    <dbReference type="GeneID" id="10530565"/>
    <dbReference type="KEGG" id="pgr:PGTG_16691"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16691"/>
    <dbReference type="HOGENOM" id="CLU_063099_1_0_1"/>
    <dbReference type="InParanoid" id="E3L290"/>
    <dbReference type="OMA" id="IIECQPA"/>
    <dbReference type="OrthoDB" id="3362246at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37487">
        <property type="entry name" value="CHROMOSOME 1, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37487:SF2">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172399" evidence="2">
        <location>
            <begin position="19"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="124"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP90665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="209" mass="20749" checksum="92DE280C9C590784" modified="2011-01-11" version="1">MSLRFLVTLATIVNLASGQASPEPPQAGDGPPLTVNTPTSVQACLPVAISWTGGTPPVYVSLITGGQVSSPILKDFGPQKDNSLTWQVDQPAGSSLSVQIRDSKGKLNYSDKFTVQSSTQTCKTEAPGSSAPSGGSTPTTPGSNNTKPGSSPTPSSSSPPSTSSATAGSPNTAATTPGSPAGQAGKSDASRAIPGFFVLAMAGALAVFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3L2S7</accession>
    <name>E3L2S7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_17042</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178338">
        <property type="protein sequence ID" value="EFP90843.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335262.2">
        <property type="nucleotide sequence ID" value="XM_003335214.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L2S7"/>
    <dbReference type="STRING" id="418459.E3L2S7"/>
    <dbReference type="GeneID" id="10530748"/>
    <dbReference type="KEGG" id="pgr:PGTG_17042"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17042"/>
    <dbReference type="eggNOG" id="ENOG502S49E">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_045165_0_0_1"/>
    <dbReference type="InParanoid" id="E3L2S7"/>
    <dbReference type="OrthoDB" id="2218151at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="92"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="123"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="166"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP90843.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="355" mass="38963" checksum="7160DE87533559FB" modified="2012-04-18" version="2">MSGFLEKLRGADFTGLNRAFGRGKHSPKIPPIGGVAYESRGISGGPLSSQDNSPQARSGRGFGRIPAENGNPTNSLLDDEDTGPTQIAIRSLTIFFLFINLFIYIAIAVFQKKWGVGPSGLTGLAIFVLVQALLHALVFLLVPQIYERTDMLRGFARAIRLTRIQFIVYGTQTGLALVLSLIATISAYTGGCKDSKSDPNASNTDYISHIGGFCRNKRAAAAFWWFSFLSFGLSLGIVFKSWYSIRKLGPRHPAFEPPQGVGVRTIDDEDDDFRADEPYDRSPPQFPQEMTAVRPSNVGGYYGERPIFDSDRVPAGYAAPSGSVHEVDHTRSPPQMAAYKDPFDEIRAQIYQNGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3L2V1</accession>
    <name>E3L2V1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_17075</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178338">
        <property type="protein sequence ID" value="EFP90876.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335295.2">
        <property type="nucleotide sequence ID" value="XM_003335247.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L2V1"/>
    <dbReference type="GeneID" id="10530784"/>
    <dbReference type="KEGG" id="pgr:PGTG_17075"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17075"/>
    <dbReference type="HOGENOM" id="CLU_108601_0_1_1"/>
    <dbReference type="InParanoid" id="E3L2V1"/>
    <dbReference type="OrthoDB" id="2495186at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP90876.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="184" mass="20859" checksum="FEC879B6EB68F1D8" modified="2012-04-18" version="2">MKQQIDYSEGPLKIYAADGTVAYRFAKVSFDSSDQGLSTTELQTDSSKVVLTLQSANDLCAQKTTYEEAHSPRSRTRRVEIYPRGMLKDVWRLNYIDDSGLRHNFKFKRGFADSGGRIYTQVNGHDGELIAELKNEKRKDSWLTQGSKSKEVRVLTLSCTEDSPMEQLIAFMALVLSRVDRCGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3A3</accession>
    <name>E3L3A3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_17300</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178340">
        <property type="protein sequence ID" value="EFP91028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335447.1">
        <property type="nucleotide sequence ID" value="XM_003335399.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L3A3"/>
    <dbReference type="GeneID" id="10528526"/>
    <dbReference type="KEGG" id="pgr:PGTG_17300"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17300"/>
    <dbReference type="HOGENOM" id="CLU_046891_2_1_1"/>
    <dbReference type="InParanoid" id="E3L3A3"/>
    <dbReference type="OMA" id="KNNEWTW"/>
    <dbReference type="OrthoDB" id="2507040at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP91028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="172" mass="19295" checksum="122D7A2273B29A47" modified="2011-01-11" version="1">MLTSKETDVLGHPTLKLTGVEQLKPPGSDSNYLDWSWILEIHFAATDVDYIINDKPEVAKSKASFARKNKAVCGIILQTIHPANIPNVQHLKNNTRGLWDSLKKAHQDSSAGGVMYWLQKLTISRMVDNDLPTHLDEMAKTFERLSALITTKSPLTPDNIYPTSILDELDRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3Q2</accession>
    <name>E3L3Q2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16368</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178343">
        <property type="protein sequence ID" value="EFP91177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335596.1">
        <property type="nucleotide sequence ID" value="XM_003335548.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L3Q2"/>
    <dbReference type="GeneID" id="10528992"/>
    <dbReference type="KEGG" id="pgr:PGTG_16368"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16368"/>
    <dbReference type="HOGENOM" id="CLU_179495_0_0_1"/>
    <dbReference type="InParanoid" id="E3L3Q2"/>
    <dbReference type="OrthoDB" id="10652178at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
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        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP91177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="102" mass="11752" checksum="072A4B5722C07E2A" modified="2011-01-11" version="1">MTSALQDDQIKAMQTTTNFRRRTGTKYSTPETRIVLPPSSAKNFSYISILLFETLKIKRSPQPTSPDHYPSYLYFASALQLLPLVTEAEVFPYRLKIYSTRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3Y5</accession>
    <name>E3L3Y5_PUCGT</name>
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        <submittedName>
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        </submittedName>
    </protein>
    <gene>
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
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            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L3Y5"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP91260.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
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</entry>
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        <submittedName>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
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                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
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                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
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                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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                <person name="Martin F."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <dbReference type="KEGG" id="pgr:PGTG_17018"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17018"/>
    <dbReference type="HOGENOM" id="CLU_2997514_0_0_1"/>
    <dbReference type="InParanoid" id="E3L489"/>
    <dbReference type="OrthoDB" id="10285135at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
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    </feature>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91364.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="57" mass="6426" checksum="9333244FD0345547" modified="2012-04-18" version="2">MLQFLRLIALVSLIAPWGVTCEDSEDDAPQERYFGCRRNVDAICTASTEDAHQQNRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3L4F5</accession>
    <name>E3L4F5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
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    <gene>
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    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
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                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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                <person name="Martin F."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178346">
        <property type="protein sequence ID" value="EFP91430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="STRING" id="418459.E3L4F5"/>
    <dbReference type="GeneID" id="10529345"/>
    <dbReference type="KEGG" id="pgr:PGTG_17386"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17386"/>
    <dbReference type="eggNOG" id="KOG2595">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_250963_0_0_1"/>
    <dbReference type="InParanoid" id="E3L4F5"/>
    <dbReference type="OMA" id="ETIEWTD"/>
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    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="999"/>
            <end position="1050"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1081"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1149"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1211"/>
            <end position="1446"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="730"/>
            <end position="826"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="116"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="158"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="197"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="231"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="418"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="458"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="513"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="524"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="551"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="567"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="626"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="652"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="871"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1083"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1156"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1182"/>
            <end position="1197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1222"/>
            <end position="1231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1266"/>
            <end position="1277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1294"/>
            <end position="1307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1357"/>
            <end position="1385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1412"/>
            <end position="1424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP91430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1457" mass="159648" checksum="BD51AA8BCD6CB6AB" modified="2011-01-11" version="1">MTAHTVTLTTTTTQNQSQPTPTSTHPLQPVLPTNTRYPSRLFTRPGKPLSAERKHLFEQAKAEVEKRCQADPVWEAERLWLLGTPKDSKEPGHLGFLRALIIRGKGHDLPQIFRTLEKGVRRRRNEATERGEPHPGPPRREVTPLTTPPARKQAAAHINTNRSTGPSTEPTSAPSQAGKPTDQPEQSPSPLALKDGQQPTSSTDPRPDQSRSSTEGSHSSATISQPDRASTHNQRQRTSSRSSRVSTDQHGSKRTETDANELTGNLPLWTPQPGTDPAQIRPKLVALADFIALPPILFPSFEKRFYSYHVVLALVHLYKDHAQNPPDSLVVKSHKLKLKMDKPWETLKEKCIEALQLISNKQAGMARDENLRSFVVPFTDLKQAQEKATKLRERAQAHAEGIFSGLLPNTIDRRSSSDPESTGDQPSSNIDPTLDSVVPGNQIISNKQLEHPGRAQATIHQESAHSGLSAPADNQTQPANPPVTTKEQPEHPTSVQHQLQATSNQESLDLDPTDARDDRVESGNKAQVQSLSPIQDRCQLTAAQESIDVDPAGRREHGVESVDPIPNHTTLPLTPSSPGVLSTSVPAGLDISRTSAPPDRVVAPVFALPHVSTTGAIVQPGPFPATPSSSSTHLSTGLARNGDPLAPGTAHSPGPMGSSMISSAVVPQNEYQPRVPELANPRLPLNYLIHSAAANPTPSSSSANNPRALHRLSIPASAQDPNLTPQQQQLQQLQLQQQLYQQKQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQHHPQQQQQQQQLQQQYQQHHQQQQQQLQLQLYQQQQQQHRQQQLQLHQQQQQQQRGNYQIQQSPIAQGYRRHSQSIAINPNHESVSVGEQLRRPIQSTLGGSTMGTLTTRPENYPLASSTSPPAGAHSLPPEISALPLTDLQLQARHSQELATLEQQFFRCIQSLEQSYAAHMSREPDPALRLHVTQLRDQRLSVGKEHYTQFMNQTRARHRAEAQHNHALAQQLRQRAPPQPVPVPASLARHPQSPTTAYTLSPAHPHSSILHPQPPAHPNERSLTHNEEVQNIRALSALSPDGGDRKRALSHASLVPHPSSTCSPSPSSSSSSSLSPSDSFRNKNLAFSHNSHDPALPGSHKRVRRPEQMSRLLEVGRTLRIDWGSGDTPVVPASSSSASSRQQQPTGIAVPPPSLSSGDRNLSINDSGNQRVVDVDLMEIDVAVEDQPSTAQKCQTREATESPDRALTVGRPVEAEDQAIDKTTTNDDRPKDSTSALEPSGPQPQSSSLPVAPSSTEVVSAAPAPVEGRHPAVDEDRRQPSADQALAVPSGPLEPAQSSTLPPTPPPNLISTQRLPEARHPATSTTHLLLPPPLPPYPPPSLSPPPPPSSSEPPPPAPSINNNTRLVRARDDQDMDLNDDPFFTISLSPNHPSSRTLHTPDVGPRLPSDVHQPRGPLDSFDSDWLYIDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3L4U8</accession>
    <name>E3L4U8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Mitochondrial distribution and morphology protein 31, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_17627</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in the organization of the mitochondrial membranes and the global structure of the mitochondria. Also required for mitochondrial distribution and mobility as well as for the maintenance of mitochondrial DNA nucleoids structures.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MDM31/MDM32 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178349">
        <property type="protein sequence ID" value="EFP91573.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335992.2">
        <property type="nucleotide sequence ID" value="XM_003335944.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L4U8"/>
    <dbReference type="FunCoup" id="E3L4U8">
        <property type="interactions" value="36"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L4U8"/>
    <dbReference type="GeneID" id="10531510"/>
    <dbReference type="KEGG" id="pgr:PGTG_17627"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17627"/>
    <dbReference type="eggNOG" id="ENOG502QQJG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016236_0_1_1"/>
    <dbReference type="InParanoid" id="E3L4U8"/>
    <dbReference type="OrthoDB" id="17678at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000001">
        <property type="term" value="P:mitochondrion inheritance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012571">
        <property type="entry name" value="Mdm31/Mdm32"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31068">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31068:SF0">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08118">
        <property type="entry name" value="MDM31_MDM32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="144"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="43"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="248"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="527"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="54"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="527"/>
            <end position="538"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP91573.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="716" mass="80724" checksum="6ED8021C5EED6BBA" modified="2012-04-18" version="2">MISYGQLSRRFPLNRLRSSGLSTGFRRFPSVILSNHSSQFKYPPFSSHPHHLKSTPTSDQDPTRSTSKIQPPNPGGIQQHDHQSLIASYLPSIRSLASKLPSNLRHPPTASDLLPLTKSTLDRLHIRLKWLTIRSFHPYRTDDYSAFASLFVLINIILAAIGTTTFLGIFLFLFNKAGMEDLVARWLSNRLSNASGVEISFDSALVPRWTDGMIRFENVRVSRGGHSLGPDAARLMSLVLDSNIPNPTPRRTLDLSPLHPDQHPTASEQESHFPEFTTPSEPDPALANCTHFHLTIASIDVTLSLGRWLDGKGLLRQAEVRGVRGVIDRSHLPAWSGESATPIDRREFRKVATAGDFHLEQVMIEDLLVTIFQPSNFRPYTFSIFNATFHKLRKQWLFLDLLSAEQMTGQVDNCLFSLHKPQSITQSNAKAGLYDQTLSSRQLENGRESYKRYKTRLSRFRIDGVPIDHLQGNSTGPGSDGPLSWINSGRVDVIADIRLPLEIDELDLRTVVREIVDNFEKELEIHQRTGDENDDAAHHPSSKRNGLISERRNLIKPHLKSPNVEESLKVDLASHHENDELLLERNDQETEEQVAIELDLRFKDLKASVPIFSSSLSYVNNALVRPIVAFINANRTLIPIKCQVKLDLEEFNGSWTTYDIGLIEIISEQVYEALAYHVGSDKVAQSNRLHKVGRWGIQMTANAILNALKQHWDAAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>E3L5D2</accession>
    <name>E3L5D2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_18557</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178366">
        <property type="protein sequence ID" value="EFP92559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336978.1">
        <property type="nucleotide sequence ID" value="XM_003336930.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L5D2"/>
    <dbReference type="GeneID" id="10538717"/>
    <dbReference type="KEGG" id="pgr:PGTG_18557"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18557"/>
    <dbReference type="HOGENOM" id="CLU_2159648_0_0_1"/>
    <dbReference type="InParanoid" id="E3L5D2"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP92559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="111" mass="12298" checksum="223FB3533593FC46" modified="2011-01-11" version="1">MPYPARGGEVREPLSPLLPSLFPRPARCRSPYKRYGSLQYAGVGKAVQLEGQVFQTTSWTATHSASPQLELNCDDPCEMWSQAREHKSTVPRTARDGLTLHKKATVSATKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3L750</accession>
    <name>E3L750_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_18360</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178363">
        <property type="protein sequence ID" value="EFP92373.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336792.2">
        <property type="nucleotide sequence ID" value="XM_003336744.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L750"/>
    <dbReference type="GeneID" id="10538037"/>
    <dbReference type="KEGG" id="pgr:PGTG_18360"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18360"/>
    <dbReference type="HOGENOM" id="CLU_717928_0_0_1"/>
    <dbReference type="InParanoid" id="E3L750"/>
    <dbReference type="OrthoDB" id="10336353at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174256" evidence="2">
        <location>
            <begin position="29"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="253"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="262"/>
            <end position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP92373.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="395" mass="43449" checksum="0F44C8F8894DF704" modified="2012-04-18" version="2">MAHWSLSKNGQLVATLACVIHAIQWIQGFPTHPHLIPSLAANISPTNWEAADEAITKIGPGPKALLMTYSHGDGIVHIPGSNPFDDQYLVYPMTEIAFHRVPTHKSWYLDDESILVHTEVIPGHAIPVQNSDLALNYDVAQTSHQTLPPKAPPTKLRKEAEPFIPRLSNAVDVRTLRRNKTCEEKAAVKTLYEPIGQQESTNTKELSNAMGRTRKGAAVPTHMTGRTDSVANLSNIHDNMREELPPEAFVACPSDLRLYPPGSSNQGSPTDNFQSKNIGKQRLDDSALTTIEDTPSLSSDKEESSTTEAEEESGTPDLAPKFDNITVLDHRKKNRDNISISGTVRLVQEPTRGARLTKSEAGRKIPLGKDQFSTSTEIQNSDNILSSMEAENICW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3L9M0</accession>
    <name>E3L9M0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Carboxypeptidase D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_19191</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178386">
        <property type="protein sequence ID" value="EFP93245.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337664.2">
        <property type="nucleotide sequence ID" value="XM_003337616.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L9M0"/>
    <dbReference type="GeneID" id="10543477"/>
    <dbReference type="KEGG" id="pgr:PGTG_19191"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19191"/>
    <dbReference type="HOGENOM" id="CLU_008523_10_3_1"/>
    <dbReference type="InParanoid" id="E3L9M0"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.410:FF:000009">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF64">
        <property type="entry name" value="CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174312" description="Carboxypeptidase D" evidence="6">
        <location>
            <begin position="20"/>
            <end position="471"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP93245.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="471" mass="52937" checksum="496854DCBDD18C2A" modified="2012-04-18" version="2">MRLYLLAFLVTVLYPSAFGVSSDCAPRSPTKKHHGKLNGQGAYRLIADSNICETTPGVHTYSGYINISQNHSLFFWFFEARNNPQSAPFTLWLNGGPGCSSLIGAFQENGPCKVNAQGNATTLNPYSWNKYAHVLYLDQPIPAGFSYGVHDVYSTDQAAHRVWQFFQYLFSIPHFSTYESRELSFWTESYGGQYGPKFTEYFEHQNDLIKSKKIEGHIVHPASLGITTGWFDPYYHYPSYYDYAQAKYNHVEDLVEPDVLAEMKKALDSPNGCVAQIKQCASTGNNTICRQADDFCTRFVSNPAVGSRNPYYFENPKSDPFPSESYVKYLSLPQVTSAIGAESKFTECPDGPNDDFTTTGDAARSSTPALAKLLNKGLRTVIWAGDRDFICNTLGGYRSISSMNWQHGEEFRKAPWSKLHLKGQIVGIYKQAGPLTYITVYKAGHEIPAYKPELALEVFRQTIFHENIHST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3LB10</accession>
    <name>E3LB10_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_19719</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178406">
        <property type="protein sequence ID" value="EFP93735.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338154.2">
        <property type="nucleotide sequence ID" value="XM_003338106.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LB10"/>
    <dbReference type="GeneID" id="10535105"/>
    <dbReference type="KEGG" id="pgr:PGTG_19719"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19719"/>
    <dbReference type="HOGENOM" id="CLU_099944_0_0_1"/>
    <dbReference type="InParanoid" id="E3LB10"/>
    <dbReference type="OrthoDB" id="2505113at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP93735.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="195" mass="22382" checksum="E3CC64B1D9F50A0B" modified="2012-04-18" version="2">MALVQRNTHERILAFSKILQAIREDDLWTAAWCMSKFLKTPHVLSPSERKLLQDDYSYRMNRVRDATNLLESLAFKHAKEDHDDWLEKGNPFPAVEEASFRKHGGNPIDRLLDEICTYSRSAGYLRAIINMKVRLPPLTSAEDGVEVFELEAMGPSPGVKNVENLDVSELDWEIIRHEEVCQGAYQGQQWTTPNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3LBS8</accession>
    <name>E3LBS8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19891</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178426">
        <property type="protein sequence ID" value="EFP94003.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338422.2">
        <property type="nucleotide sequence ID" value="XM_003338374.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LBS8"/>
    <dbReference type="GeneID" id="10535618"/>
    <dbReference type="KEGG" id="pgr:PGTG_19891"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19891"/>
    <dbReference type="HOGENOM" id="CLU_044243_0_0_1"/>
    <dbReference type="InParanoid" id="E3LBS8"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="317"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="92"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP94003.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="364" mass="40923" checksum="BE4AFFB26E89C4A3" modified="2012-04-18" version="2" fragment="single">ELQETSRLIKYYLNFLASEKAQRAAAYQPTPWELLTPQEQRARALKRVEINKENRINNLFAFNSLKRQHKNRRREILAELKEKRLAKMSVISSSSAATTPAAHTTAHSPVSGPTTPTCTDPSLQRPDLAVASQLEERVNRLKLITIQKKLAPIATKATVTLDPNGLAPLTNLATTLAEKTAVPAMTSATIAQDRMDLDDAAQKTPVQNTNPATIASEAMDVDPIASTFSVDIQLLRKLPEKQVLSKEERIKLLVKEHIHLWKRCTVAQQSGAMEDLKILLHQAQDSQKVLQKLIPRKELEEFVKGWNPWTIKKELFPTAPKKNDGKKRSSSSKGAKELYNDPNRWKMVMRGCNTLEAAYRHMAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3NXJ6</accession>
    <name>E3NXJ6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_20243</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS989945">
        <property type="protein sequence ID" value="EFP94295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338714.1">
        <property type="nucleotide sequence ID" value="XM_003338666.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NXJ6"/>
    <dbReference type="GeneID" id="10527421"/>
    <dbReference type="KEGG" id="pgr:PGTG_20243"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20243"/>
    <dbReference type="HOGENOM" id="CLU_026798_3_0_1"/>
    <dbReference type="InParanoid" id="E3NXJ6"/>
    <dbReference type="OrthoDB" id="2505311at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="156"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP94295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="416" mass="47860" checksum="B0155FAABBE78CC9" modified="2011-01-11" version="1">MSTPPLARFDLHDISNLTPHGRKKRNARTTRRRRTKREKKCAEEFTTARPPTQFRIIQKKLRPIDLFPDITADFNQRKAEQKQLVEAHKKDPKNIPKPCEKQIFARNPTTEENNLALETVKDSFSFINSKYNKIYDENSSKLVALVEFLKFEDLTEEQWDDLNFLCLFLQDCKEFISPVASKSRKCAGIMWALGWRKDYDGIEILGRYRDKKAIENNPEGFEKVMNRSIRAGEVLWKTFHGFADVAVEKNQKFMEDFNIPSIADNNFPESPGEKYPFGFASNLAFSSHGFYNHHHLDSGDSSELPLAFALIIPTSKLTGKIANDDYDVTNGQFIFRDLRIALDFKPRTICRMIFRAQEYVHGTLLPTEPTVFTKLGLALQVATRTSNACKLYLNGTFNDDTDIYFGGVDALLEKNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPG4</accession>
    <name>H6QPG4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Capsule-associated protein CAP1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20844</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EHS63862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890557.1">
        <property type="nucleotide sequence ID" value="XM_003890508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPG4"/>
    <dbReference type="STRING" id="418459.H6QPG4"/>
    <dbReference type="GeneID" id="13543175"/>
    <dbReference type="KEGG" id="pgr:PGTG_20844"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20844"/>
    <dbReference type="HOGENOM" id="CLU_440143_0_0_1"/>
    <dbReference type="InParanoid" id="H6QPG4"/>
    <dbReference type="OrthoDB" id="202415at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="366"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="640" mass="73169" checksum="72A810472CD8B188" modified="2012-04-18" version="1">MSDIYLLSESSGSRKSSLVESLFKSDEPSTFPLSPKLRHDHLIGFSHRRLGSRGSSFSRVHSYLSQLANVEEEEKIATGLRVKTLRRRLRMPKYQALLLLVAGVLVYQCVFRSTSPKTTPSASPAAETTTPLVVLPMVSLDDPHPLRKIIQDAQESWKTIQKRQSKTYPEAVLEYQRRYKINPPNGFDKWFSFAKSHGHVLTDEYDSMMEQLSSFRKMDRNELHSRTNLIGKAQGFGLIVMKNGRINAQIPNNLSYSTYTNNDHENHEFNLQEGRIYTRWATSDKPAHKEIKQTQTWAFSKLIEKITRLDPTLFQDMSLAINEHFEPRVIIPWETKENSDEDLSQSWNHNRFEEHDNVWTSFRKACPPSSEARKQNDGPNFPEELLPSSSIEGKWEDGPQRAALEESLTANRSPLRFVTESPAPIKDIYCDQPQRHLEQGLFFSNTSKIEGLFPVFSQSTAQGFGDIVIPSYLYIRDPYKTREYANEPVSKRSRGTSFIAGGQASKMDPSLWKPWDVKWAQKKDQIYWRGLLRDGGDRPSGFQSNFGRHRLVKRFGSSVRSRSESLVWDSSPTKQSIFGSKDSGLNSLMGIGTLNPKMLSKQVLDRSFVNHKTMDVGYLAEDHCRAHEPCQELLKGKLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPL0</accession>
    <name>H6QPL0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20874</name>
        <name evidence="3" type="ORF">PGTG_20877</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EHS63892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EHS63895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890587.1">
        <property type="nucleotide sequence ID" value="XM_003890538.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890590.1">
        <property type="nucleotide sequence ID" value="XM_003890541.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPL0"/>
    <dbReference type="GeneID" id="13543180"/>
    <dbReference type="GeneID" id="13543208"/>
    <dbReference type="KEGG" id="pgr:PGTG_20874"/>
    <dbReference type="KEGG" id="pgr:PGTG_20877"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20874"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20877"/>
    <dbReference type="HOGENOM" id="CLU_1540853_0_0_1"/>
    <dbReference type="InParanoid" id="H6QPL0"/>
    <dbReference type="OrthoDB" id="2515405at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="126"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS63895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="174" mass="19619" checksum="FCE8E35A0ED02310" modified="2012-04-18" version="1">MEVRSNTFSHRITDDDVKNTIPDISQRKESVVEQCYNVCRNFGELDWEENLYAPGLSHCNHDPLTGYLRNVRNNQSFHNSFGNNAMIQNPLAQSHGWMYSSNGMQTNGMHNNTNQNSRQSSFNSYQSGPNGGNQNFNQGNKRPRGGYKGSNYSDNFVGRSDGGQSSGRKNNQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>H6QR74</accession>
    <name>H6QR74_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21396</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EHS63056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889986.1">
        <property type="nucleotide sequence ID" value="XM_003889937.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QR74"/>
    <dbReference type="GeneID" id="13540496"/>
    <dbReference type="KEGG" id="pgr:PGTG_21396"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21396"/>
    <dbReference type="HOGENOM" id="CLU_983983_0_0_1"/>
    <dbReference type="InParanoid" id="H6QR74"/>
    <dbReference type="OrthoDB" id="2506334at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="130"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="283" mass="31649" checksum="7147680436F48376" modified="2012-04-18" version="1">MAAPPTAKKSMPYIASSPSRRQMLDDSPNTDDGLPDTTNAQESMHRVYYMLSTGKKSLMIGMIELYAFVTALEKDWVQVQRGIPIAYGSLPAKQVNISQSVGWNKATKRQKAAWNDGRAPDTTEQLLAASEQPMKRAKLGRPKNSPNVNKDPQSTYVSYGASTSKLARNRCWLAAALESLYAVYSPLWLQEPGGKQNDLFFVLVSHFTTRSTYEMCKSPQIRSILTRGSNKIFIETQKLYPLNFISGAFSSCDLFLELVLDVKKNKSKTLPKLFCVQENRCGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>H6QS73</accession>
    <name>H6QS73_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Anthranilate synthase component I</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21683</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EHS63507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889652.1">
        <property type="nucleotide sequence ID" value="XM_003889603.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QS73"/>
    <dbReference type="STRING" id="418459.H6QS73"/>
    <dbReference type="GeneID" id="13542888"/>
    <dbReference type="KEGG" id="pgr:PGTG_21683"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21683"/>
    <dbReference type="HOGENOM" id="CLU_1240668_0_0_1"/>
    <dbReference type="InParanoid" id="H6QS73"/>
    <dbReference type="OrthoDB" id="1865897at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="entry name" value="Anthranilate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236">
        <property type="entry name" value="AMINOBENZOATE/ANTHRANILATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236:SF9">
        <property type="entry name" value="ANTHRANILATE SYNTHASE COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00095">
        <property type="entry name" value="ANTSNTHASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="ADC synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chorismate-utilising enzyme C-terminal" evidence="1">
        <location>
            <begin position="104"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="223" mass="24041" checksum="33018ED93E5123B1" modified="2012-04-18" version="1">MPRFESSRKTVFLMDRVAETPSPVRTPFFFSHGMRILTASGQQPDRITPESIISGSFSTLRQCTGVSAMLTADRLPAGQICQGRGWSVCPANTSPGCGRAAVVVYIFYINCGQAVQLIGASPECLYKLSPGGVVKNHAIAGTIRRGKSLAEDEVLAQELLGSIKDRAEHVMLVDLARNDVNRVCWPETVKVDSLMAVEKFSQVMHLTSQVSGVLREGLTSAAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSG2</accession>
    <name>H6QSG2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21778</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178294">
        <property type="protein sequence ID" value="EHS63699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889521.1">
        <property type="nucleotide sequence ID" value="XM_003889472.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSG2"/>
    <dbReference type="GeneID" id="13542761"/>
    <dbReference type="KEGG" id="pgr:PGTG_21778"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21778"/>
    <dbReference type="eggNOG" id="KOG4306">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_554470_0_0_1"/>
    <dbReference type="InParanoid" id="H6QSG2"/>
    <dbReference type="OrthoDB" id="1046782at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="492" mass="55244" checksum="FFACF197FACDA320" modified="2012-04-18" version="1">MNSDQQTISSSTSSASSSTTNSLRHHSNSSLSLSSLNKNKNKNTKKTIRPSQSIFNLASATLQFTTPFTPDNSSIKLKIDNQTTDASIRIAQWPTKFKKSNHIKQSSYHLSSYRGTCFNPNSIPPVMKEFTQRSITLAEIDQPSGLFESSRTSKTRLGWIYFDLIKPNGYVLHLQAYVKLGRGGTTISEGLLGRFDLDSSRERPMPSGLIGSVEILSSLNLVLFKLTQEEIEKEENTVDDTPLKPPIGLPTKRLSTFSYVSHPQIILSFRVGKDARYHQHQHVSPSSNLIPKFIRSDHTLGGLNSLRALLYIQHSNTIEPILHRSASINPLSGLVTEGDKIHRLASTADTKSVFLPELDLSISYGFHDSRMGKRHSMYAYATPNYSHWLGDLIDQDQRWLDVRFSKLVLQGSHDSGMFTSLHPGFVKMITKMKLDSDIGNFLIDHGISFVHILTKLLKTFNIDLELAICNIANTQKDVIFDQLRHGTRKFHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSW8</accession>
    <name>H6QSW8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21941</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178301">
        <property type="protein sequence ID" value="EHS63922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889390.1">
        <property type="nucleotide sequence ID" value="XM_003889341.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSW8"/>
    <dbReference type="GeneID" id="13540665"/>
    <dbReference type="KEGG" id="pgr:PGTG_21941"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21941"/>
    <dbReference type="HOGENOM" id="CLU_2639275_0_0_1"/>
    <dbReference type="InParanoid" id="H6QSW8"/>
    <dbReference type="OrthoDB" id="1607513at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="77" mass="8647" checksum="C04B5F831DD8D8BE" modified="2012-04-18" version="1">MPTQQQSQCSPSKTPSIDDSSESNPDKVTIIETSGPSAAQKTKMSTQDKQSSWVWQNFQTQAIDGKKWNTTKDLWKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>H6QT31</accession>
    <name>H6QT31_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="5">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_21988</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="5"/>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EHS63985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889347.1">
        <property type="nucleotide sequence ID" value="XM_003889298.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QT31"/>
    <dbReference type="STRING" id="418459.H6QT31"/>
    <dbReference type="GeneID" id="13541892"/>
    <dbReference type="KEGG" id="pgr:PGTG_21988"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21988"/>
    <dbReference type="HOGENOM" id="CLU_011478_1_0_1"/>
    <dbReference type="InParanoid" id="H6QT31"/>
    <dbReference type="OrthoDB" id="5409596at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="71"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="499"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="625"/>
            <end position="645"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="499"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="538"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS63985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="645" mass="71299" checksum="2E900B643B626161" modified="2012-04-18" version="1">MATNNIPVLLLSATCRPVAVSAITSSLMLQPSDIQMIDGELTRPEIRLIRVTMKSTLSSCDDLLRLFAPYTVTSARETVPMIIYSGTRNRTFQVMKVVNEARNTPHHEYDPNDQFIRRYHSVNSDEDKARVMDDFGGGKVPVISATMALGLGQNLKRVRCVVHMGRGDPSAIGQMVGRCGRDGNVGLGLLFMEPTRKNGRNSVAEFTPGLEQNDDMRMDALAVTSVCLRIALNLDNKCGYIPLDPNDPNVLQEQAREDRLGFQKCKCSCCAPQEAECLMNVIQQTTVGNFDSILNDPWSVPKDPRIVTMKRKLRSHIPKGTCCYPKDVAEDLVQHLLASFEVFYVDVLGPRPEFLPSVFFGIDEARAIVATIDQICAGDLLNQTLLERVIGGQSFKGQIESHSSAIMTWMNGELYKLHLHNITQLDMFIEAEGIRVRQAMADELSGLQEVAAARRHADLELKRLKKSEAKSQAAEARAAARLSKAADLAVEKARLLSEKAADKARREEEKLARLKSEAQTRAAEKATIKAQRAAENSNKAETKGGKKQRKPQDWASKKAARQLQLVRNLAARKTACLDTSKCVDEMSQEDDITMDNGIAGGINDGNAKGQEYNQVQQLFQPPVVFPGVDEDRSAGRAGRVAKPYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>H6QT77</accession>
    <name>H6QT77_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21996</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178308">
        <property type="protein sequence ID" value="EHS64031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889280.1">
        <property type="nucleotide sequence ID" value="XM_003889231.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QT77"/>
    <dbReference type="GeneID" id="13542030"/>
    <dbReference type="KEGG" id="pgr:PGTG_21996"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21996"/>
    <dbReference type="HOGENOM" id="CLU_556837_0_0_1"/>
    <dbReference type="InParanoid" id="H6QT77"/>
    <dbReference type="OrthoDB" id="2507050at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="426"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="453"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="473"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="485"/>
            <end position="497"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="515" mass="57287" checksum="D630C186240BFEA9" modified="2012-04-18" version="1">MSPNQTNVSPAAATQAPSTTNNNATDNTSVERLLVPETTQRDASEEADVVIPNDRANTADKNPTKRKSRAVKGKGRAPAPAAAKTRKGLAPPSKVLPSEASLRETVGEEAFLGLGDDEEETSDSDEDHPLSQVTIKMNHNEILRKNIEVATLRGDMESVEMLEGLLHDEIVEQSQPERNARPLPKFKRNPTKTGPNPYAIPESSAVAPLALSVVAKKQPDTPSIPITQGGLTFMAGVVTSHADIGFTPFFNKNIRELRSPLPLTIFDKDWQERALTYHVEKRSKSDDTSNERVSRYSGLPYPNEWSQTYASWSMNHKTFHELLRDVYDLSVFAGWITIHKQHVDNLHKHHGFMPAFRYNMMTRANTFSHRVYDRNGTEMVPDISKFKTDIWQICYSQSQKHQELGFTDNPYAKGGIRENWDPLTGEERRAKPNHSYNQPFHSASHYGGQPSRNDSRNDNPHPSNHAETRGDASSSRSSGYSNSGFRGGSSRGRGGGRSNWSGRGSDNVTEKKRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTA9</accession>
    <name>H6QTA9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22021</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178310">
        <property type="protein sequence ID" value="EHS64063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889242.1">
        <property type="nucleotide sequence ID" value="XM_003889193.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTA9"/>
    <dbReference type="GeneID" id="13541673"/>
    <dbReference type="KEGG" id="pgr:PGTG_22021"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22021"/>
    <dbReference type="HOGENOM" id="CLU_2832400_0_0_1"/>
    <dbReference type="InParanoid" id="H6QTA9"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="66" mass="7720" checksum="12C5A145AB310602" modified="2012-04-18" version="1">MKLTEWLRSNVELPLESFQFPQTSFINSIQNPSNSLRYQKQEENHIPGRKVESRTGQVQDQGSQRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTE9</accession>
    <name>H6QTE9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22074</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178312">
        <property type="protein sequence ID" value="EHS64164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889209.1">
        <property type="nucleotide sequence ID" value="XM_003889160.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTE9"/>
    <dbReference type="STRING" id="418459.H6QTE9"/>
    <dbReference type="GeneID" id="13541720"/>
    <dbReference type="KEGG" id="pgr:PGTG_22074"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22074"/>
    <dbReference type="HOGENOM" id="CLU_1125009_0_0_1"/>
    <dbReference type="InParanoid" id="H6QTE9"/>
    <dbReference type="OrthoDB" id="2150628at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051667">
        <property type="entry name" value="Archaeal_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37096:SF1">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37096">
        <property type="entry name" value="YALI0E33429P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase" evidence="1">
        <location>
            <begin position="54"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="247" mass="27668" checksum="5190A78D7CBA73C0" modified="2012-04-18" version="1">MYAVLRQASCFVSVCTGNNQRTFRRGPVALSPFPSRTSAQSFYNTYGSEHAARFYNRPREVKELVIRLKDEPTFTVLLGPPSSGKTALARAVTSLRRADQTPEFHPLTIDLRAADTMKNGSFLNAFLLQGQRHAIEGGWWNKWLSGLEVSAGGVSAKFGNKTVFPASAGNIFDDLATQLKPWDLVHGGRPPIIVVDEANFFKRIEDDAFLDFAVRITKQEMKMHIIFTSSDSFFVNWLQIFISQSNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUX6</accession>
    <name>H6QUX6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22527</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178362">
        <property type="protein sequence ID" value="EHS62589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888688.1">
        <property type="nucleotide sequence ID" value="XM_003888639.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUX6"/>
    <dbReference type="GeneID" id="13542205"/>
    <dbReference type="KEGG" id="pgr:PGTG_22527"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22527"/>
    <dbReference type="HOGENOM" id="CLU_2198220_0_0_1"/>
    <dbReference type="InParanoid" id="H6QUX6"/>
    <dbReference type="OrthoDB" id="2507887at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003606452" description="Secreted protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="100" mass="10994" checksum="BA72E032CFCAEC5D" modified="2012-04-18" version="1" fragment="single">MLLWMTMYTLALPGLLRAQGLQLPPADSPACIHLQREPVETTLPCPILYNCHDGHRHVCQIELNASGQRCTGCGDVDDEEYLETCRLGATTHPTVPCPGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>H6QV46</accession>
    <name>H6QV46_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_22610</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178372">
        <property type="protein sequence ID" value="EHS62707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888619.1">
        <property type="nucleotide sequence ID" value="XM_003888570.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QV46"/>
    <dbReference type="GeneID" id="13542311"/>
    <dbReference type="KEGG" id="pgr:PGTG_22610"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22610"/>
    <dbReference type="HOGENOM" id="CLU_167023_0_0_1"/>
    <dbReference type="InParanoid" id="H6QV46"/>
    <dbReference type="OrthoDB" id="2507259at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="64"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="99"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS62707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="119" mass="13082" checksum="5378CEB56D6FF96A" modified="2012-04-18" version="1">MARTEFLELADIATLALQIDNELHGLNPTAEPTKTPDPNTMDLSVMRGRLSDAEKTRMMRAGQCFRCENQGHISRDCPDRGLKGKGKEKSRIAKLEEEVKQLKAGGSRADLSKNGGAQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>H6QVI6</accession>
    <name>H6QVI6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Small EDRK-rich factor-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_22758</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178434">
        <property type="protein sequence ID" value="EHS63043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888479.1">
        <property type="nucleotide sequence ID" value="XM_003888430.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QVI6"/>
    <dbReference type="GeneID" id="13540998"/>
    <dbReference type="KEGG" id="pgr:PGTG_22758"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22758"/>
    <dbReference type="HOGENOM" id="CLU_176397_1_1_1"/>
    <dbReference type="InParanoid" id="H6QVI6"/>
    <dbReference type="OrthoDB" id="73348at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1050.10">
        <property type="entry name" value="At2g23090-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045230">
        <property type="entry name" value="MBS1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026939">
        <property type="entry name" value="ZNF706/At2g23090_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21213">
        <property type="entry name" value="GEO09665P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21213:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN 706"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF118359">
        <property type="entry name" value="Expressed protein At2g23090/F21P24.15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS63043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="73" mass="7840" checksum="7E59B9B2A1AEE3C1" modified="2012-04-18" version="1">MVRGHAKAQAQAKNAAKQGSQKGSQLGAQQAVTKVGCSICKAPTPSYKLLVTHYEAKHPKESVPTEETFKTMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>H6QVK7</accession>
    <name>H6QVK7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Terpene cyclase/mutase family member</fullName>
            <ecNumber evidence="7">5.4.99.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_22774</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the terpene cyclase/mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="7"/>
    <dbReference type="EMBL" id="DS989930">
        <property type="protein sequence ID" value="EHS63115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890894.1">
        <property type="nucleotide sequence ID" value="XM_003890845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QVK7"/>
    <dbReference type="FunCoup" id="H6QVK7">
        <property type="interactions" value="78"/>
    </dbReference>
    <dbReference type="STRING" id="418459.H6QVK7"/>
    <dbReference type="GeneID" id="13541033"/>
    <dbReference type="KEGG" id="pgr:PGTG_22774"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22774"/>
    <dbReference type="HOGENOM" id="CLU_009074_2_1_1"/>
    <dbReference type="InParanoid" id="H6QVK7"/>
    <dbReference type="OrthoDB" id="21502at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000250">
        <property type="term" value="F:lanosterol synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016104">
        <property type="term" value="P:triterpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02892">
        <property type="entry name" value="SQCY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.20:FF:000003">
        <property type="entry name" value="Terpene cyclase/mutase family member"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.120.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032696">
        <property type="entry name" value="SQ_cyclase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032697">
        <property type="entry name" value="SQ_cyclase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018333">
        <property type="entry name" value="Squalene_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002365">
        <property type="entry name" value="Terpene_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008930">
        <property type="entry name" value="Terpenoid_cyclase/PrenylTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01787">
        <property type="entry name" value="squalene_cyclas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11764:SF20">
        <property type="entry name" value="LANOSTEROL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11764">
        <property type="entry name" value="TERPENE CYCLASE/MUTASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13243">
        <property type="entry name" value="SQHop_cyclase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13249">
        <property type="entry name" value="SQHop_cyclase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01016">
        <property type="entry name" value="Prenyltransferase_Like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48239">
        <property type="entry name" value="Terpenoid cyclases/Protein prenyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01074">
        <property type="entry name" value="TERPENE_SYNTHASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0752">Steroid biosynthesis</keyword>
    <feature type="domain" description="Squalene cyclase N-terminal" evidence="9">
        <location>
            <begin position="144"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="domain" description="Squalene cyclase C-terminal" evidence="8">
        <location>
            <begin position="407"/>
            <end position="741"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS63115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="756" mass="86707" checksum="C6925D47C9F5308A" modified="2012-04-18" version="1">MLNRQDTAIEDTVIAELVETDLSRWHLDSSREGQVAWTYDTSMGREMGEQSFETKYWLSIQQQTPLPPDPKGNPLHAARNGFEFLKRIQSPDGHWSSEYGGMSAYFQGSIVNLYPSQHLKISLHRLIISCYVTKTVLPEEFKIELLRYLIHHQRQNHDVCDQGWGLNVSQKSTVLGTALNYVACRLLGLDAQVPMLARARNTLHALGGAACIPTWGKVWLSVLNVYDWEGINPTPPESWLLPSFLPFHPSRWWVHSRQVYIPMSYLAGRKAKAELDPLLKALRKELYIQPYEDIDWLSCRSLISETDLHSPHHPALKCAFIMLSYWEKICPQRLREMGLNHVHQLCRMEDEDTDFIHLGPINKLFNLLVCWDRDGPGSKAFVRHQRGLGDYLWMTNKGMFMMATNGSQLWDLSFITQAVVESGIAETEDKPSQETLVKALGWLDRCQILENPKHYHNSHRHRTRGAWPFSTKKQSYTVSDCTAEALKSVMLLQQELNYTPELVTKERLYMAIEVILGLQNSDGGFASYELTRGPKWLEWLNPSELFANAMIEYSYTECTTACLTAMSMFTKYHPEFRDSEIKQAIHSALKFVHSTQREDGSWHGSWGVCFTYATMFGLESLSVNQENYENSTRAQKACKFLLDRQMVDGGWSEMFESCAQKFYMQGTESQVVQTSWAILGLLAVKYPDHRPIKRACKLLMDKQKLSGDWEDHSIAGSSNETIIVTYPLFNFSWPICALGKAYKQFGDLLIDDGRTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQS6</accession>
    <name>E3JQS6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_00664</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74708.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307714.2">
        <property type="nucleotide sequence ID" value="XM_003307666.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQS6"/>
    <dbReference type="STRING" id="418459.E3JQS6"/>
    <dbReference type="GeneID" id="10546659"/>
    <dbReference type="KEGG" id="pgr:PGTG_00664"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00664"/>
    <dbReference type="eggNOG" id="KOG0017">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012390_7_0_1"/>
    <dbReference type="InParanoid" id="E3JQS6"/>
    <dbReference type="OrthoDB" id="2506822at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023:SF4">
        <property type="entry name" value="GLYCINE-RICH PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="2">
        <location>
            <begin position="181"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="334"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="380"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP74708.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="409" mass="45933" checksum="BBAB3C6F26978A76" modified="2012-04-18" version="2">MGDSTNPLDSNKTPKCSDNLATPKPAEKSTNNTDAAIDTTPNDTAIDTTPKADENTTMKKGFPCWSFEEDKRLCIAWLNTSRDAIVGKGQKATTFWEQIHETLSDLINEYKEEKKHTRFFKELPLRPVGAVECRWGIILKTVNKFSGCYSNVERCLKSGKTREDILTEAKELYKATFGSSFNLDHCWGILKDAPKWQATQQESEARGKKAKDVPSTASAPSSDPPASTPVVSSPAVIEVEEEDSENSRSALGNVCIGGQKSAKRKQAEECAIEKIVGMQKELVQISRDRLASMKSALQSTSDNAIMSANLNTMDDESRAYYQKKQRAIIARELQEEKEREEKEKKQKEEKDKKEKEEKEKKEKERKEKEEKQRKDKEVIEIDGEDEDKDNDSGTNEEKSGKDNSAAGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRI6</accession>
    <name>E3JRI6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00571</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74615.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307621.2">
        <property type="nucleotide sequence ID" value="XM_003307573.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JRI6"/>
    <dbReference type="GeneID" id="10527482"/>
    <dbReference type="KEGG" id="pgr:PGTG_00571"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00571"/>
    <dbReference type="HOGENOM" id="CLU_1134044_0_0_1"/>
    <dbReference type="InParanoid" id="E3JRI6"/>
    <dbReference type="OrthoDB" id="10279983at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172078" evidence="1">
        <location>
            <begin position="19"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP74615.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="245" mass="27644" checksum="C5B276B3A569AF42" modified="2012-04-18" version="2">MSLSHLLCFLLWLQLTIACLYPEDDWLAAFAERLAADIEVDSSGNLDQSFTYEEGTHLKRARPSDIYDCNELNPSCPEALSGCMQEFHPIIIPSDQPTQGSGSPQPKRACKDQSILLQKLPHNHHETAKGIGFWTDIKILSANELNDAFQKMLNIDRPRRGVSTLSGVFGLLRSELKASLNTNQDEEYGHVANQYQKYYQETINDIYLETQFLPVGTESLKIDIIGTPFEFDGGKIWTRNVSAEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3JS31</accession>
    <name>E3JS31_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_01495</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP74902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319321.1">
        <property type="nucleotide sequence ID" value="XM_003319273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JS31"/>
    <dbReference type="STRING" id="418459.E3JS31"/>
    <dbReference type="GeneID" id="10547100"/>
    <dbReference type="KEGG" id="pgr:PGTG_01495"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01495"/>
    <dbReference type="eggNOG" id="ENOG502SCIP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031560_0_1_1"/>
    <dbReference type="InParanoid" id="E3JS31"/>
    <dbReference type="OMA" id="HYESDEP"/>
    <dbReference type="OrthoDB" id="2506424at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="336"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP74902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="427" mass="47148" checksum="C1B6507B76E0F029" modified="2011-01-11" version="1">MPPSLLPQGGDSATSNDMSGMDMYVTEGSLPTVENTLAERLTKLLDTLIPRMESLERVVSGQASKPTGATRADSFNTLLVRIEEIAAKMTRLEKKVEALSSPLSSNMMAQTTAKPSILPPRPAPLYSEKAAGISKPGAAKSLPLVPPSLYINRFKLGQVVIRKKFDQPKPFEGLTAAAICQKINDALDFAKATIDNEVIKVKAVAQFPNGDVKIFTKDRRAAKWLLDNKHLWTEKADPVFVTSQVAHPVLLHSCPTSFEVDNKEHVELLCKQNNIEPKEIQKIRWLLNPKGTGKASSTLLIFFFNRRLALDIERAGLCYNSLHLKGQHYLQGPKQCYNCLAVGHLAHSCKEKPLCSRCGAGHNSASCDEMEDSSRNCQRCLQVDRKSSTTVDLSDPKYDHSPFSSACPIRAKEIEKLSKDKTPNPRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>E3JSH8</accession>
    <name>E3JSH8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_01544</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP74951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319370.1">
        <property type="nucleotide sequence ID" value="XM_003319322.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JSH8"/>
    <dbReference type="FunCoup" id="E3JSH8">
        <property type="interactions" value="405"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JSH8"/>
    <dbReference type="GeneID" id="10547233"/>
    <dbReference type="KEGG" id="pgr:PGTG_01544"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01544"/>
    <dbReference type="eggNOG" id="KOG0730">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_12_3_1"/>
    <dbReference type="InParanoid" id="E3JSH8"/>
    <dbReference type="OMA" id="DRHIYVA"/>
    <dbReference type="OrthoDB" id="27435at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19511">
        <property type="entry name" value="RecA-like_CDC48_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000661">
        <property type="entry name" value="calmodulin-interacting protein 111 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003455">
        <property type="entry name" value="Nuclear vcp-like-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF27">
        <property type="entry name" value="ATPASE FAMILY GENE 2 PROTEIN HOMOLOG A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00830">
        <property type="entry name" value="ENDOLAPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="203"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="478"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP74951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="718" mass="79023" checksum="6716289E0B83D8EE" modified="2011-01-11" version="1">MINIGIKPGELINITPLPPILPPNHIGPGRGIRLPEAKLIKLKQTKPPKEYQDLIPTTITISTTTTTQSSDRLWEGFIREVLVDRKYLAINHYIIIPYANQLRFYHIQDIQSTTTTTTTTNPSDSLELFFITRNTRLEIIKPSTPSKPSTTTTTTTQSLLTQSADQIGYEAIGGLQSQIEQIREMVELPLTRPELYSHFGIKPPKGILLYGPPGTGKTHLASIIAKSTNSTLLTLSTATISSAYHGEGEQKIFEIFAEAKEKSPSMIMIDEIDGLFPSREIGSEVDRRMVGALLTCMDGMEEKNDDPGLDQEENPRVIVIGTTNRPQTLDPALRRPGRFDRELEIGIPNGGARLEILQVLLRKTPHQLTAAEIKEVADKTHGFVGADLLALIREAGLGAIKRSLATHIALQDMHLQPQDLSQALLKIRPSAMRELLVETPTTRWTDIGGQTAIKQRLLETLKWPAEHPQTFKRLGISPVRGILLYGPPGCSKTLIAKALATESGLNFIAIKGSDVFNKYLGESEKSIRDLFKKARANSPSIIFIDEIDSIAMARAGSDDLGGGGGTGVGDRVLTSLLVEMDGIEELNGVLVLAATNRPEVIDPALMRPGRLDRILYVGPPDLDSRIEIFKINFRKMSVDPSLDIHKLATLTDGSTGAEIVAICQDAAINAIHRDFNVAFIVEEDFLKSIQKIAKKQAINSQLIQHYEKWRDLVGVQSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3JSX1</accession>
    <name>E3JSX1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_01739</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319565.1">
        <property type="nucleotide sequence ID" value="XM_003319517.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JSX1"/>
    <dbReference type="STRING" id="418459.E3JSX1"/>
    <dbReference type="GeneID" id="10547588"/>
    <dbReference type="KEGG" id="pgr:PGTG_01739"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01739"/>
    <dbReference type="eggNOG" id="ENOG502R2F4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_778750_0_0_1"/>
    <dbReference type="InParanoid" id="E3JSX1"/>
    <dbReference type="OMA" id="NEHQNEI"/>
    <dbReference type="OrthoDB" id="2507620at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="231"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="150"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="163"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="186"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP75146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="356" mass="39793" checksum="6A35A9B89C283A6B" modified="2011-01-11" version="1">MGKEIEEGENSKPSTGKQELDQHQSATSSPPELPPKTAIQSYPSIITTTNHSKQSNKILIRFLFSLSNFIFFGGTLAGLIVWFYQKYIFPKLRIRTEILKSLRLSTLKSYDKLHQSLKSLIQSNPRLYQSTLAKLSGKVDQVLSTSSDAKTGDGLDDAGKASDQIQVSEGSSTTDEKTQVPDIVPSSSSSTSSSSILDEKELQNEPHKPSSDREEFNQPVIDYLEKIAQGLRKRSDNRRPVQIEESDDEQMDESRAGTERSLGGIESLTKSLKSMGAELADEAQLNTRVHQKLSASSHPHRAFGLNHWTASFNATRYPDAPPPKNVDEDPYFIALMEFKNQIRNLKGVLLNRKKFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>E3JT42</accession>
    <name>E3JT42_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histone H3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_01862</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="8">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the histone H3 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319688.1">
        <property type="nucleotide sequence ID" value="XM_003319640.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JT42"/>
    <dbReference type="STRING" id="418459.E3JT42"/>
    <dbReference type="GeneID" id="10542734"/>
    <dbReference type="KEGG" id="pgr:PGTG_01862"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01862"/>
    <dbReference type="eggNOG" id="KOG1745">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078295_4_0_1"/>
    <dbReference type="InParanoid" id="E3JT42"/>
    <dbReference type="OMA" id="FLPMART"/>
    <dbReference type="OrthoDB" id="2502034at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000001">
        <property type="entry name" value="Histone H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11426">
        <property type="entry name" value="HISTONE H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00322">
        <property type="entry name" value="HISTONE_H3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00959">
        <property type="entry name" value="HISTONE_H3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="7 8" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="10">
        <location>
            <begin position="46"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="19"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP75269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="138" mass="15555" checksum="5BA7753738D976BB" modified="2011-01-11" version="1">MARTKQTARKSTGGKAPRKQLAAKAARKSAPSSVTAGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQESAEAYLVGLFEDTNLAAIHAKRVTIQPKDIQLARRLRGERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3JTL5</accession>
    <name>E3JTL5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01983</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319809.1">
        <property type="nucleotide sequence ID" value="XM_003319761.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JTL5"/>
    <dbReference type="STRING" id="418459.E3JTL5"/>
    <dbReference type="GeneID" id="10542937"/>
    <dbReference type="KEGG" id="pgr:PGTG_01983"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01983"/>
    <dbReference type="HOGENOM" id="CLU_785144_0_0_1"/>
    <dbReference type="InParanoid" id="E3JTL5"/>
    <dbReference type="OrthoDB" id="308861at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1730">
        <property type="entry name" value="tricorn interacting facor f3 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042097">
        <property type="entry name" value="Aminopeptidase_N-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037813">
        <property type="entry name" value="TAF2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15137">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15137:SF9">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR TFIID SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63737">
        <property type="entry name" value="Leukotriene A4 hydrolase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="381" mass="42678" checksum="5A28BBA3422D1469" modified="2011-01-11" version="1">MSSHGPQNRAFSVTHQRAILDIKLDGTINAITEITILPLSADLNTVHLHSSHCTISSIYHPSQHPNNNKQPLQFILNQPNLVKIPDPNSVRQFPEAKPRLFEQVNKKETGELAIQIDRHKINRTSNRQPATSLLPPSDQHQLPAQKDKDLEFDPIIITIEYQITQADLTLNPAICVVGGQHPHLFTNSISARSWVPCLDSLWERSPWELEFIVPRSSITNHAKPSPLTVVASGELIEQITHPTDPSKTVFYYAQAIPTSVQHIAWVVGPLTVNDLTPYRPPPTSSPHQDKVDQPVDQEIITDQHHQSSTEINLLAFCLPNRQSQLLHVVNFLPHALFFFTQEGLFLDMSAATHTICATFNSATLTLHPSDLLYPPQVIDQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3JTX3</accession>
    <name>E3JTX3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transmembrane protein 14C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_00858</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM14 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319946.1">
        <property type="nucleotide sequence ID" value="XM_003319898.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JTX3"/>
    <dbReference type="FunCoup" id="E3JTX3">
        <property type="interactions" value="21"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JTX3"/>
    <dbReference type="GeneID" id="10543243"/>
    <dbReference type="KEGG" id="pgr:PGTG_00858"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00858"/>
    <dbReference type="eggNOG" id="KOG4267">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_096652_4_0_1"/>
    <dbReference type="InParanoid" id="E3JTX3"/>
    <dbReference type="OMA" id="ANSHKIM"/>
    <dbReference type="OrthoDB" id="5620at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1740">
        <property type="entry name" value="Transmembrane protein 14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005349">
        <property type="entry name" value="TMEM14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044890">
        <property type="entry name" value="TMEM14_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12668:SF43">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 14 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12668">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 14, 15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03647">
        <property type="entry name" value="Tmemb_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP75527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="105" mass="11021" checksum="5D326A8C963789D5" modified="2011-01-11" version="1">MPSTSPDYLGFAYGFLVATGGIMGFVKASSVPSLVAGTVSGGLIALGAHRYQQKGKPDLIFGMSVMLTLLMGKRFLASRKMMPAGMTTTLSLVMAVRYGVKLLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3JU50</accession>
    <name>E3JU50_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00906</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319994.1">
        <property type="nucleotide sequence ID" value="XM_003319946.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JU50"/>
    <dbReference type="GeneID" id="10543291"/>
    <dbReference type="KEGG" id="pgr:PGTG_00906"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00906"/>
    <dbReference type="HOGENOM" id="CLU_136933_1_0_1"/>
    <dbReference type="InParanoid" id="E3JU50"/>
    <dbReference type="OMA" id="AKDWFKQ"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="122" mass="13418" checksum="2817882A1FDB9278" modified="2011-01-11" version="1">MTTRSNTDPDELLPLTDPEAIIRSGNAEQRRLKHLKSNPTIPHPPPSETTPAIAMSDDTAPAHETSGSTRTADAADMQTAKDWFKPNVDKLQRIADKLWRIAARTDRSLFLLIKPAPPALAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUD3</accession>
    <name>E3JUD3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cullin family profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_00989</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the cullin family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320077.1">
        <property type="nucleotide sequence ID" value="XM_003320029.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JUD3"/>
    <dbReference type="FunCoup" id="E3JUD3">
        <property type="interactions" value="579"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JUD3"/>
    <dbReference type="GeneID" id="10543458"/>
    <dbReference type="KEGG" id="pgr:PGTG_00989"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00989"/>
    <dbReference type="eggNOG" id="KOG2167">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004747_7_3_1"/>
    <dbReference type="InParanoid" id="E3JUD3"/>
    <dbReference type="OMA" id="NYQEQTW"/>
    <dbReference type="OrthoDB" id="27073at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080008">
        <property type="term" value="C:Cul4-RING E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006974">
        <property type="term" value="P:DNA damage response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000014">
        <property type="entry name" value="Cullin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.130:FF:000043">
        <property type="entry name" value="Cullin-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1310.10:FF:000037">
        <property type="entry name" value="Related to cullin 4A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1310.10">
        <property type="entry name" value="Cullin Repeats"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.130">
        <property type="entry name" value="Cullin, Chain C, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045093">
        <property type="entry name" value="Cullin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059120">
        <property type="entry name" value="Cullin-like_AB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016158">
        <property type="entry name" value="Cullin_homology"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036317">
        <property type="entry name" value="Cullin_homology_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001373">
        <property type="entry name" value="Cullin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019559">
        <property type="entry name" value="Cullin_neddylation_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016159">
        <property type="entry name" value="Cullin_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11932">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00888">
        <property type="entry name" value="Cullin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26557">
        <property type="entry name" value="Cullin_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10557">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00182">
        <property type="entry name" value="CULLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00884">
        <property type="entry name" value="Cullin_Nedd8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75632">
        <property type="entry name" value="Cullin homology domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74788">
        <property type="entry name" value="Cullin repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50069">
        <property type="entry name" value="CULLIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0832">Ubl conjugation</keyword>
    <feature type="domain" description="Cullin family profile" evidence="7">
        <location>
            <begin position="548"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="19"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="38"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP75658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="938" mass="106352" checksum="AAB03A8D4B63A573" modified="2011-01-11" version="1">MKRTNPESRPSFGQHHETTNTNNNNRSNPNNQRTNPQDNPQASLQTFRAATASKRNRIHHQPPSPIEVEEPPLTTLSNSFNPIKTELPTAFVLGLSSTGPQPIQPLLPQINSIFEISKISIRNLKKRPQDTCGAPLDGFYQNSLSNLRHALDDILHVPPKPTETSLTTLSEACQGLVLAGPRWCESLYKQVKDQLENKTLEIRAYLLEGMPTHPVMDALLSSNSVDIIKAIASFDQPPCHAWLEKTHAVWHDWYHQLRLIRSMLVHLDRFILARSHEMLPIWELGLDLFRKNVIGRPWNPISLSLSVVICQQVTLERSGQTIPVGLLQSLTQLVFTAFGATGFTNLISTSLQKATESFYTQEGMRLIQDVESNVLSVGGPAGYLSHIKNRLESEVELFNKIFTTPDRALNAQLLNSILRLVESNLILVHLETLLTHGLVRLLESFPDPIAATSLLTFYKLLTRLGEPPVRRLRKSFSVWIKATGARMVEKANGGEEEEAKRDAGMVERLIEFKTKLDGIVVGCFAEDREMFFAIKEAFETFINQRCNKPAELIAKYLDLKMRTASRSMNEAEIDTCLEHVLVLFRYSQAKDIFEEFYKRDLAKRLLLSRSSSIDLERNMVMKLKKECGPGFTAKLETMFRDLETSNDLNIAYESVLAREAGGEERMEEEEEEGDRVELTVTVLTSGSWPMSQASEPKALLPTHLQTHLSRFEKFYGSKYLGRRLTWAHSLGQVVLIASFPKNNNNMTNSRPPASFVHAATTRKELTVSTIQALVLLLFNVDTDNLSIDFQSIVQRTGIDEKTAARTLQSLACGKVRVLVKNPKSKEVSKTDRFTFNSNFKDEHFKIKINQIQSKETVEERSSTRDKVVTDRATLIQLSIVRIMKSRKKSKFNPLLFEVIEGLKSRFQVDVKDVKLAIENLISRDYLERLSVDEFHYLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUR5</accession>
    <name>E3JUR5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">UDP-glucuronate decarboxylase</fullName>
            <ecNumber evidence="5">4.1.1.35</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGTG_01121</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Nucleotide-sugar biosynthesis; UDP-alpha-D-xylose biosynthesis; UDP-alpha-D-xylose from UDP-alpha-D-glucuronate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">Golgi stack membrane</location>
            <topology evidence="2">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NAD(P)-dependent epimerase/dehydratase family. UDP-glucuronic acid decarboxylase subfamily.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.35" evidence="5"/>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75790.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320209.2">
        <property type="nucleotide sequence ID" value="XM_003320161.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JUR5"/>
    <dbReference type="STRING" id="418459.E3JUR5"/>
    <dbReference type="GeneID" id="10543782"/>
    <dbReference type="KEGG" id="pgr:PGTG_01121"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01121"/>
    <dbReference type="eggNOG" id="KOG1429">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_3_0_1"/>
    <dbReference type="InParanoid" id="E3JUR5"/>
    <dbReference type="OrthoDB" id="331544at2759"/>
    <dbReference type="UniPathway" id="UPA00796">
        <property type="reaction ID" value="UER00771"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032580">
        <property type="term" value="C:Golgi cisterna membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048040">
        <property type="term" value="F:UDP-glucuronate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042732">
        <property type="term" value="P:D-xylose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033320">
        <property type="term" value="P:UDP-D-xylose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044516">
        <property type="entry name" value="UXS-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43078:SF6">
        <property type="entry name" value="UDP-GLUCURONIC ACID DECARBOXYLASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43078">
        <property type="entry name" value="UDP-GLUCURONIC ACID DECARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="13" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0520">NAD</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="15">
        <location>
            <begin position="2"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="170"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="177"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EFP75790.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="241" mass="27088" checksum="2BA74A47158A53C4" modified="2012-04-18" version="2">MNMLGLAKRTKARFLLSSTSEVYGSPEQHPQKETYWGNVNPIGPRACYDEGKRVAEALTYGYARENGVEVRVARIFNTYGPRMSPSDGRLVSNFIMAAIRGEPLEVYGDGQQTRSLMYVFDLVDGLIKLMNSENHAEVHEGPVNLGSEDEQPVIEWARLIIDIVNNLKSHHPHHHSKASEEPSKSSVIVHKDPLPDDPPRRRPDSSKAKLFLDWTPKWSAKAGLEDTAKFFFDLVHSGYSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3JV19</accession>
    <name>E3JV19_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01225</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP75894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320313.1">
        <property type="nucleotide sequence ID" value="XM_003320265.1"/>
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    <dbReference type="AlphaFoldDB" id="E3JV19"/>
    <dbReference type="GeneID" id="10532809"/>
    <dbReference type="KEGG" id="pgr:PGTG_01225"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01225"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172147" evidence="1">
        <location>
            <begin position="20"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="111" mass="12378" checksum="E075D816744A2AB5" modified="2011-01-11" version="1">MHHTRLLSLIKVFIISSEANDPVPWFANQDYLAICIACHFAEWVTRSYATPIVFPSASITQTSYMDLRACSLMMPTTICGPLLYASEKLLEGTSAETRSCIQSTSDLRTNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>E3JV36</accession>
    <name>E3JV36_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 subunit J</fullName>
            <shortName evidence="4">eIF3j</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Eukaryotic translation initiation factor 3 30 kDa subunit homolog</fullName>
            <shortName evidence="4">eIF-3 30 kDa subunit homolog</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">HCR1</name>
        <name evidence="6" type="ORF">PGTG_01242</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the eukaryotic translation initiation factor 3 (eIF-3) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the eIF-3 subunit J family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320330.2">
        <property type="nucleotide sequence ID" value="XM_003320282.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JV36"/>
    <dbReference type="FunCoup" id="E3JV36">
        <property type="interactions" value="56"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JV36"/>
    <dbReference type="GeneID" id="10532989"/>
    <dbReference type="KEGG" id="pgr:PGTG_01242"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01242"/>
    <dbReference type="eggNOG" id="KOG4813">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_085806_0_0_1"/>
    <dbReference type="InParanoid" id="E3JV36"/>
    <dbReference type="OrthoDB" id="20381at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0033290">
        <property type="term" value="C:eukaryotic 48S preinitiation complex"/>
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        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005852">
        <property type="term" value="C:eukaryotic translation initiation factor 3 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001732">
        <property type="term" value="P:formation of cytoplasmic translation initiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.60">
        <property type="entry name" value="Eukaryotic translation initiation factor 3 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03009">
        <property type="entry name" value="eIF3j"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023194">
        <property type="entry name" value="eIF3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013906">
        <property type="entry name" value="eIF3j"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21681:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT J"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF08597">
        <property type="entry name" value="eIF3_subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="28"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="34"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP75911.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="254" mass="27679" checksum="6AC76D0ABF05BAAB" modified="2012-04-18" version="2">MSDWEASDHEEAANAGISVAAGVSAIKKNKWADEDADEDVKDDWEASESEDEKSKNEPAKPKGPVRNKGITKMKIAEKEAEEALRLAEQEALAQQAADPVLRKKLEQTRTIQSDMENARGLFGDAVVSNPNSSEDPMKMDPKTKADFESMSSALAQLIVSKHGNKPLYPLFVEHFFRQLSEPLSDVQTRKTASILTTIANEKQRAAKDAKKGKGKLKPTLGGGVVSGASKGGRGVNADLEVYDQALDDEFDDFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>E3JVP3</accession>
    <name>E3JVP3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="15">Saccharopepsin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGTG_02559</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Vacuole</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the peptidase A1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320537.1">
        <property type="nucleotide sequence ID" value="XM_003320489.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JVP3"/>
    <dbReference type="FunCoup" id="E3JVP3">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JVP3"/>
    <dbReference type="MEROPS" id="A01.018"/>
    <dbReference type="GeneID" id="10533522"/>
    <dbReference type="KEGG" id="pgr:PGTG_02559"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02559"/>
    <dbReference type="eggNOG" id="KOG1339">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013253_3_4_1"/>
    <dbReference type="InParanoid" id="E3JVP3"/>
    <dbReference type="OMA" id="KYDHDAS"/>
    <dbReference type="OrthoDB" id="771136at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000036">
        <property type="entry name" value="Vacuolar aspartic protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.70.10:FF:000002">
        <property type="entry name" value="Vacuolar aspartic proteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001969">
        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033121">
        <property type="entry name" value="PEPTIDASE_A1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF51">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00026">
        <property type="entry name" value="Asp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00792">
        <property type="entry name" value="PEPSIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00141">
        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51767">
        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="8 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 12" id="KW-0645">Protease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 13" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0926">Vacuole</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172183" evidence="13">
        <location>
            <begin position="25"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A1" evidence="14">
        <location>
            <begin position="114"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="319"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="145"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EFP76118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="430" mass="46832" checksum="FC259B49284A756D" modified="2011-01-11" version="1">MKSTSAVVAITALAAVASIPSATAGKHRMKLHKMPITSSANSQTILNNLQSQTAWVSQKYFGVDDTASEKKFRYGHALKQPKEGDDVSIQMIEEAELASAGHEVPLSNYLNAQYFSEISLGTPPQSFKVVLDTGSSNLWVPSTRCTSIACFLHSKYDCEASETYQANGTEFKIRYGSGSLEGVISNDVLTIGDLTVPDVDFAESTKEPGLAFAFGKFDGIFGLGYDTISVLHTVPPFYKMMENGMLDDPVFAFYLGSAQGNKADPNGGEVVFGGVDEAHYEGEIFYAPVRRRGYWEVELKSVKFGKEEMKLHNVGAAIDTGTSLIALPTDTAEIINAEIGATKSWSGQYTVDCSRIPELPDLTFNFGGKEFTITGEDYILQVSGTCVSAFTGLDMPPNIGELWIVGDVFLRKWYTVYDWGRDAVGFAKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3JW10</accession>
    <name>E3JW10_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">L domain-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_02676</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320654.1">
        <property type="nucleotide sequence ID" value="XM_003320606.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JW10"/>
    <dbReference type="STRING" id="418459.E3JW10"/>
    <dbReference type="GeneID" id="10533965"/>
    <dbReference type="KEGG" id="pgr:PGTG_02676"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02676"/>
    <dbReference type="eggNOG" id="KOG0619">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042564_0_0_1"/>
    <dbReference type="InParanoid" id="E3JW10"/>
    <dbReference type="OMA" id="GRICYFQ"/>
    <dbReference type="OrthoDB" id="2501785at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001611">
        <property type="entry name" value="Leu-rich_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025875">
        <property type="entry name" value="Leu-rich_rpt_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003591">
        <property type="entry name" value="Leu-rich_rpt_typical-subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050216">
        <property type="entry name" value="LRR_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48051:SF36">
        <property type="entry name" value="CASPASE FAMILY P20 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12799">
        <property type="entry name" value="LRR_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00369">
        <property type="entry name" value="LRR_TYP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52075">
        <property type="entry name" value="Outer arm dynein light chain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51450">
        <property type="entry name" value="LRR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0433">Leucine-rich repeat</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="30"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="186"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="466"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="48"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="108"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="469"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP76235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="604" mass="67278" checksum="926E2083AF4D36F1" modified="2011-01-11" version="1">MQAPSTHSNVVTDPFSAIYKARPGELPTSLSIESYHNKRVEQQEALLDQQSADHSSLASKTTEKSDHSSSNRLQNVFCPSLAGERDRGVRKRMLSNPESLLPPPPMNTSGSIRPSSKLSASTVVHDPDDVNITKDTQEEVDSKKKFQFPAETANPNPFESSFHTPPRRCGIFFSPPRSLDTLTSKRTRSENFTRSAHRLQPSEEETEATLRSESHHRFSSAFSRAIIRGDICLDLDSQDLHTIPDLEKLVEEQKTVVGHLQDKVKVMALIEKMVNPSGTSESGGVRQSLTRVKSAPSVSFAPRSTASLFLSNNQLTSSPFGQLDRICYFQGLEQLSLRSNRLTEIPEAIGKLKNLKVLNLAYNQLEFLPSSILKLTDCKLLLNGNPWLKPPLPLDSSSPLKTTLGNRTVQQFRWLSESQGVFYFSPPARTLVPIDDSLPAPLPSLLETCIRKICISKDLSGKAETLIGQDDDDDDDDDLVHDHLCPPSSSTESPHRSTELTTLLPARIQKIIKQPASYFYRCDLCTTKLVLKPGALPHPPSTQTSPHPRHAFQFRTLGLLGLLPFPQPQSHTPDQKIELDHNNFIPIRWNICSLNCCIEHLINT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWL1</accession>
    <name>E3JWL1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">glucan 1,3-beta-glucosidase</fullName>
            <ecNumber evidence="7">3.2.1.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_02877</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Successive hydrolysis of beta-D-glucose units from the non-reducing ends of (1-&gt;3)-beta-D-glucans, releasing alpha-glucose.</text>
            <dbReference type="EC" id="3.2.1.58"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.58" evidence="7"/>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320855.1">
        <property type="nucleotide sequence ID" value="XM_003320807.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JWL1"/>
    <dbReference type="FunCoup" id="E3JWL1">
        <property type="interactions" value="17"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JWL1"/>
    <dbReference type="GeneID" id="10534492"/>
    <dbReference type="KEGG" id="pgr:PGTG_02877"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02877"/>
    <dbReference type="eggNOG" id="ENOG502QRG8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004624_6_1_1"/>
    <dbReference type="InParanoid" id="E3JWL1"/>
    <dbReference type="OMA" id="PLSEPWN"/>
    <dbReference type="OrthoDB" id="62120at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004338">
        <property type="term" value="F:glucan exo-1,3-beta-glucosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000033">
        <property type="entry name" value="Glucan 1,3-beta-glucosidase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050386">
        <property type="entry name" value="Glycosyl_hydrolase_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297:SF34">
        <property type="entry name" value="GLUCAN 1,3-BETA-GLUCOSIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31297">
        <property type="entry name" value="GLUCAN ENDO-1,6-BETA-GLUCOSIDASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="168"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="745"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="804"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="88"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="822"/>
            <end position="838"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00036824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP76436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="838" mass="91796" checksum="7E17B537C44046EC" modified="2011-01-11" version="1">MGPSSPPPIGSLPAPPRLMTAPTPVDQHLHDSYPPRRSSVSPSPEPEELTHQLPPDSRPHSPYLPPLPHNPFAYDPPPLKDFPRRPSYQRNSSATWNTTSDSDFTRIGYDSWIHTSGSMVALDKLDETEIEGIGLVHPSNTKRVETENDSFYVSSAPPANKPSKRRNLLISLAGLALLVLAIIIGTVVSMRKSRLADLGKVNPGDGTLVTASGNVIKLWGKDGDKITTDNGTTFEYKNPLGGTWVAIPFNDTAKPQADSPALNQPWDYSNRRILGVNLGGWLVLEPFITPYMFEPFSSHDANGQAPTVVDEWTLSVALGDKLASTLEEHYRTFITEEDFMQIAAAGLNWIRLPVGWWMIETWDGEPFLEGVSFKYFLKALQWARKYGLRVNLDLHAVPGSQNGFNHSGKLGSINFLVGLMGVANAQRTLNYIRTLTQFISQPQYVNVVPMFSVLNEALVQKIGVPQMRSFYLQAYQMIRGITGYGAGKGPMMIIHDGFQGTGAGHTGWAGFLSGADRIGLDTHTYFAFDKQSNDSLGYNSYKPCTYWAKGFNQTNTNFGFNFAGEYSLAVNDCGLWLNNIGVGSRYDGTYPTANAPDTTNFPMVGSCDPWKDYRTWTPDMKKSIADLAATSQDAMQNSFFWTWKISRSIRTPDLKPNPMWDYQLGLQEGWIRPDARGSIGACGAVAAQQGATIPAQPWAGKFADWQVGAGGSDPRTIDPAQLQQYGQWPPSQIFVQQGQNAVYPDTSNLPQYVPTGSPVVLKPDAPNPHDSPASTSVDPGSGWANNADKAGWYVPVKDCPYPNPWAGGGLPVPSSPFCGGTPTPQLVSKTSSPSLRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3JX66</accession>
    <name>E3JX66_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02102</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76641.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321060.2">
        <property type="nucleotide sequence ID" value="XM_003321012.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JX66"/>
    <dbReference type="GeneID" id="10528579"/>
    <dbReference type="KEGG" id="pgr:PGTG_02102"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02102"/>
    <dbReference type="HOGENOM" id="CLU_1361015_0_0_1"/>
    <dbReference type="InParanoid" id="E3JX66"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="109"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="180"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76641.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="201" mass="21980" checksum="30C3C60E18334C30" modified="2012-04-18" version="2">MFIFYIGISHLIEKPIPQVLGSPMQTTMRHTSPLVPHDVTSPLVPHDVNGQAIIPKRLPGAESLELVRVDRGKLVFPGNLVDCDMAWYGPPKRVTKPYAPIKQAKSKPLSAAAPAFHPAPKTSSAGLLGPHPRNPETMEKLRIEGSQYYRSRGTPNVFAGANTRGYNQPRKNMDPKAEKSSSSGELPSPLGNRDFKQGRAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXK9</accession>
    <name>E3JXK9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02245</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76784.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321203.2">
        <property type="nucleotide sequence ID" value="XM_003321155.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXK9"/>
    <dbReference type="GeneID" id="10528886"/>
    <dbReference type="KEGG" id="pgr:PGTG_02245"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02245"/>
    <dbReference type="HOGENOM" id="CLU_1993739_0_0_1"/>
    <dbReference type="InParanoid" id="E3JXK9"/>
    <dbReference type="OrthoDB" id="10477994at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76784.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="125" mass="13940" checksum="1D63D3C85A004CC7" modified="2012-04-18" version="2">MSHPVVGDPEWDQLQCNYDPLGEHTPLTGRFSNTTVSDLQPFSLATPSTTSAPHHTNPPRGHPHPQTTTASNPPLSLLESVHRNRRWRLATRRPHSRLGHATLQLGHATLQHVAPMPKPMWNFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXS7</accession>
    <name>E3JXS7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HTH TFE/IIEalpha-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_02313</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TFIIE alpha subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321271.1">
        <property type="nucleotide sequence ID" value="XM_003321223.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXS7"/>
    <dbReference type="FunCoup" id="E3JXS7">
        <property type="interactions" value="361"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JXS7"/>
    <dbReference type="GeneID" id="10528957"/>
    <dbReference type="KEGG" id="pgr:PGTG_02313"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02313"/>
    <dbReference type="eggNOG" id="KOG2593">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035744_0_1_1"/>
    <dbReference type="InParanoid" id="E3JXS7"/>
    <dbReference type="OMA" id="DAIKWKV"/>
    <dbReference type="OrthoDB" id="361102at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005673">
        <property type="term" value="C:transcription factor TFIIE complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000269">
        <property type="entry name" value="Transcription initiation factor IIE subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039997">
        <property type="entry name" value="TFE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017919">
        <property type="entry name" value="TFIIE/TFIIEa_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002853">
        <property type="entry name" value="TFIIE_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024550">
        <property type="entry name" value="TFIIEa/SarR/Rpc3_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097:SF7">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE, ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02002">
        <property type="entry name" value="TFIIE_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00531">
        <property type="entry name" value="TFIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51344">
        <property type="entry name" value="HTH_TFE_IIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH TFE/IIEalpha-type" evidence="5">
        <location>
            <begin position="38"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="309"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="358"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="13"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="358"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="375"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="404"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP76852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="484" mass="53267" checksum="17AF590C1A08C15D" modified="2011-01-11" version="1">MTMPSSAASSPSLQPGFSSGNSTSPTSPEDQPDPLADLRLLLQFVARAFYEVRHILLFDQLIRKEAMKDEELSARLGMTPKDLAKAANPLIRDQLVSTYMRAEQKPGAYKATQRTYYFIDYKHCVDVIKWRMWKMNKVLDDKQRNVIDGQGYVCPRCKKTYSTLDISGLSMTATSFLCEICGTPLNDNENDIEVQKNKDRMQRLNSQTMTIKKNLQKADQIVIPPFDIAKWVAVNVPLENQVDSSDGLAVATGGAKEAAVTVQLAEEGDLEAKKRLKEQQAEAKRQANALPVWIAQSTVSGELTRAGMKDGPMMSNVQQSSGVSFHDPSAVVQSQSSENQIDDIDYDAYFAKLQTQTGSTACSSTGSNDSKRSPADSSAGSLPSPTHSRDSPIAAGLKRPRAADQSNSYYGSHSHNERASKKSRPGMPLIGADRTPPEHPPSVDVRMVMVAGKPIPLLEVTDDHEQLMSSEEYTQYAQLVLQDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3JYA4</accession>
    <name>E3JYA4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02490</name>
        <name evidence="3" type="ORF">PGTG_17036</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP77029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="DS178338">
        <property type="protein sequence ID" value="EFP90837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321448.1">
        <property type="nucleotide sequence ID" value="XM_003321400.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335256.1">
        <property type="nucleotide sequence ID" value="XM_003335208.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JYA4"/>
    <dbReference type="GeneID" id="10529376"/>
    <dbReference type="GeneID" id="10530742"/>
    <dbReference type="KEGG" id="pgr:PGTG_02490"/>
    <dbReference type="KEGG" id="pgr:PGTG_17036"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02490"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17036"/>
    <dbReference type="HOGENOM" id="CLU_2016373_0_0_1"/>
    <dbReference type="InParanoid" id="E3JYA4"/>
    <dbReference type="OrthoDB" id="2508532at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="57"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP90837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="123" mass="13643" checksum="C52D587FA198E99B" modified="2011-01-11" version="1">MLLMTLCKAAVLGRLTQVNQWVANQALKSSLTQIQTSESDDSDHPFRKVPKTLVVIQHDNKEEKPQIVSSKETSPEALIDAQSELPKDDDNGTPAETRPLLSPPIKPESEPETEHTTISMEQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3JYF5</accession>
    <name>E3JYF5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PPM-type phosphatase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_03036</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77080.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321499.2">
        <property type="nucleotide sequence ID" value="XM_003321451.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JYF5"/>
    <dbReference type="STRING" id="418459.E3JYF5"/>
    <dbReference type="GeneID" id="10529428"/>
    <dbReference type="KEGG" id="pgr:PGTG_03036"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03036"/>
    <dbReference type="HOGENOM" id="CLU_1525923_0_0_1"/>
    <dbReference type="InParanoid" id="E3JYF5"/>
    <dbReference type="OrthoDB" id="416093at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00481">
        <property type="entry name" value="PP2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="2">
        <location>
            <begin position="46"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="100"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP77080.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="176" mass="18568" checksum="346F8471609F7FFA" modified="2012-04-18" version="2">MGVDKRLLLSFLLADQQLISRCPKSGIEDAEQSGTIHPHFSSPLLSLVVAHLGNTSALLCSAIDGKVLPLTEYHHPDSRVESDRLRRIGTGLITDSFGESPWGTSSSGGAPPGSVASLGPGYPLGYPDIRQVWAEKQPSEAESAPAGGYPLALAGIRQRIADIRNGLSPPISRVHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>E3K023</accession>
    <name>E3K023_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Isocitrate lyase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_03604</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate</text>
            <dbReference type="Rhea" id="RHEA:16809"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:57429"/>
            <dbReference type="EC" id="4.1.3.30"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>D-threo-isocitrate = glyoxylate + succinate</text>
            <dbReference type="Rhea" id="RHEA:13245"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:36655"/>
            <dbReference type="EC" id="4.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="12">Can also use Mn(2+) ion.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322067.1">
        <property type="nucleotide sequence ID" value="XM_003322019.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K023"/>
    <dbReference type="FunCoup" id="E3K023">
        <property type="interactions" value="68"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K023"/>
    <dbReference type="GeneID" id="10538856"/>
    <dbReference type="KEGG" id="pgr:PGTG_03604"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03604"/>
    <dbReference type="eggNOG" id="KOG1260">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019214_2_2_1"/>
    <dbReference type="InParanoid" id="E3K023"/>
    <dbReference type="OMA" id="YVSGWQV"/>
    <dbReference type="OrthoDB" id="4078635at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004451">
        <property type="term" value="F:isocitrate lyase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046421">
        <property type="term" value="F:methylisocitrate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006097">
        <property type="term" value="P:glyoxylate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00377">
        <property type="entry name" value="ICL_PEPM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.850:FF:000001">
        <property type="entry name" value="Isocitrate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039556">
        <property type="entry name" value="ICL/PEPM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006254">
        <property type="entry name" value="Isocitrate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018523">
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    </dbReference>
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        <property type="entry name" value="BIFUNCTIONAL GLYOXYLATE CYCLE PROTEIN"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR21631">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001362">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00161">
        <property type="entry name" value="ISOCITRATE_LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0329">Glyoxylate bypass</keyword>
    <keyword evidence="7 9 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="204"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="95"/>
            <end position="97"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="205"/>
            <end position="206"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="241"/>
        </location>
        <ligand>
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        </ligand>
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    <feature type="binding site" evidence="11">
        <location>
            <begin position="419"/>
            <end position="423"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="454"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001050"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004793"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005704"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00017446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022435"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR001362"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PIRSR" id="PIRSR001362-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001362-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP77648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="536" mass="59412" checksum="E0EFA5DBEE130BC7" modified="2011-01-11" version="1">MADKEQQIFEQEVNSLKAKWASPRYKGLVRPYTAEQVVGKRGTLPISYPSEVVGKKLWNLLNQHYKNGTPSHTYGALDPIQVTQMAKYLETVYVSGWQSSSTASSTNEPGPDLADYPSNTVPNKVEHLFMAQLFHDRRQREARLRMTPEERKKTPPVDYIRPIIADADTGHGGLTAVMKLAKMFVEKGAAGIHIEDQAPGTKKCGHMAGKVLVPISEHINRLVAIRLQFDIMGVENIVVARTDAEAATLLSTNVDERDHGYILGTTNPNVGHLVDLMNRAQSEGKSGDALQSIEDEWTLKANLVLYSDAVAAELKVRGAPTNKIADWVKSSMGLSHTAAKALAKKSYGIEIFWDWDMPRTREGFYRYQGGTGCAINRAVAYAPYSDLLWMETKSPILAQAQEFAKGVRSVWPDQWLAYNLSPSFNWDAAGLSTEQMKSYVWELGKMGFCWQFITLGGLHSNAYISDLFAKSFAKEGMKAYVEEIQRKEREIGCEVLTHQKWSGADYADNLLKTCTGGVSSTAAMGKGVTESQFGGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3K1Y6</accession>
    <name>E3K1Y6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04311</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003322726.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K1Y6"/>
    <dbReference type="GeneID" id="10542309"/>
    <dbReference type="KEGG" id="pgr:PGTG_04311"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04311"/>
    <dbReference type="HOGENOM" id="CLU_863667_0_0_1"/>
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    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="283"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="284"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="327" mass="36564" checksum="B1C062A43BBC178A" modified="2011-01-11" version="1">MLENHQQQPIDCLLDHLISISTQLIDSSKNALESTINSRDCLSTFSRIENLLDRSIRIRQRELNRKIHSIQSHQLELDRIQEQVHSLWPQQQQQQQQQHHHLHFHQVINHYPSIHHQKQSSNNTLISIEEDQTLKPHQPTLRLSPSLKIKPIQLTRSNSCNNKEHYQSPTVQLLSPSVPSVRSDHSSILLDYHARRSFDQPAPLHPVFPPSQPTTHSNTSSSSSSSSSSLATFFSPLRIAKKASNILSSSFLSSTLNHPPTATTIVPSRLSIDSTLNELASRDPSSLLAHQSSPSSSPASSNLSLPPPVPFSSTTPQLTPSDLLHPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2D4</accession>
    <name>E3K2D4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">B30.2/SPRY domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_04459</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the cclA family.</text>
    </comment>
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        <property type="protein sequence ID" value="EFP78503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003322874.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2D4"/>
    <dbReference type="FunCoup" id="E3K2D4">
        <property type="interactions" value="439"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K2D4"/>
    <dbReference type="GeneID" id="10536184"/>
    <dbReference type="KEGG" id="pgr:PGTG_04459"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04459"/>
    <dbReference type="HOGENOM" id="CLU_014420_2_0_1"/>
    <dbReference type="InParanoid" id="E3K2D4"/>
    <dbReference type="OMA" id="DDSHAIE"/>
    <dbReference type="OrthoDB" id="10266026at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048188">
        <property type="term" value="C:Set1C/COMPASS complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd12872">
        <property type="entry name" value="SPRY_Ash2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.920:FF:000043">
        <property type="entry name" value="Protein TRAUCO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037353">
        <property type="entry name" value="ASH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001870">
        <property type="entry name" value="B30.2/SPRY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043136">
        <property type="entry name" value="B30.2/SPRY_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003877">
        <property type="entry name" value="SPRY_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10598">
        <property type="entry name" value="SET1/ASH2 HISTONE METHYLTRANSFERASE COMPLEX SUBUNIT ASH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10598:SF0">
        <property type="entry name" value="SET1_ASH2 HISTONE METHYLTRANSFERASE COMPLEX SUBUNIT ASH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00622">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00449">
        <property type="entry name" value="SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50188">
        <property type="entry name" value="B302_SPRY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B30.2/SPRY" evidence="5">
        <location>
            <begin position="120"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="314"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="535"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="24"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="349"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="546"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="597"/>
            <end position="617"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP78503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="617" mass="67389" checksum="060B3860BCA3E371" modified="2011-01-11" version="1">MSPNADVSEPLVPESIPVEEPIVVDERDEHSASERYETDGEQSTNVESIPPKAISGPPTGEAEAKPRLLSTHIDSATVAIPPVFQPLPSFFPPDFFATRSHPWNRSGFRYLACGPPVDQQHQDVDYPVHYTIETAPPGLVKWSWEDRSSYVYVTKDGLSVTTDRGFRSARANLPIREGQWYFEISIVRAGGEINQGARSEPEEAHVRVGIARRESGLNAPLGIDGYSYGIRDKTGEKVHLSRPQKYGEPFGSGDVIGILIDLPKMRQPRDGDHDDPARIERKRIPIRYRRQLYFESAEYPISKEMEELAESALPAKLKSKQEPHKATLTSETPKKKSLPGQKQVEDEPPTLPALPSGPPLRPLSVLKGSKVAFFKNGVCQGVAFRDLLDFLPLRVDPSPQNNGSMHQLGEDQTAADSNNVKSGGRLYDDGTLGYYPCVSVYGGGIARLNAGPEFKFAPPDSVNHLLDEEADLPEKDTSNDSTGTSEKTWKPLSELYPIHLAEQRRLDELDIIEAQKQLAESVQRASLAQVDVTVKPKATSKRKRVSSPSSSAVGSSGTAKGKKKGKVGSTSDGLLPQTFKPSPLGNMEIIEEPQPTPSTQSTGEPGTGTHSPAIGSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2K6</accession>
    <name>E3K2K6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04531</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78575.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322994.2">
        <property type="nucleotide sequence ID" value="XM_003322946.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2K6"/>
    <dbReference type="GeneID" id="10536340"/>
    <dbReference type="KEGG" id="pgr:PGTG_04531"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04531"/>
    <dbReference type="HOGENOM" id="CLU_011714_1_0_1"/>
    <dbReference type="InParanoid" id="E3K2K6"/>
    <dbReference type="OrthoDB" id="2509187at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="315"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="383"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78575.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="392" mass="42517" checksum="E095D9FC075A8733" modified="2012-04-18" version="2">MSTLERLDLYEKVQALAAHPALKPNERGHIQALLNDIKADYENSDDEDNTGGPEVTNIQRSPATPVGEERPQTAVHSFNLPAAKSPAGPTGSESYLAPEPQAAKDNRTPNQSPSRAPRIGEFFILPNPIGAPRTTCPTFSQFSSGNTTSPVPADINMDSHMQDGQHSENSRSTLNTLGQTTFLTRAGNEFLESIEQNHAVLNKRVLVTPASNTASTTSLTTAVLSNSSTSAPVGLHRIHPKKTVIHEPRSRPTQYRFPMVQLEGMDDDLAAKRARLERRGSHEDADGSTDDEFDHTDKQPSQGQVEVVLDNGLGRAADFRQGPNGPPPQASRPSEFLTDSMFRNYVIPRRPHEGTGANLNDTRPRADQSQNSSGGPTPLRSARSPSDLNLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>E3K352</accession>
    <name>E3K352_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Malate dehydrogenase</fullName>
            <ecNumber evidence="3 12">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGTG_04865</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 12">
            <text>(S)-malate + NAD(+) = oxaloacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. MDH type 1 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="3 12"/>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323328.1">
        <property type="nucleotide sequence ID" value="XM_003323280.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K352"/>
    <dbReference type="STRING" id="418459.E3K352"/>
    <dbReference type="GeneID" id="10537097"/>
    <dbReference type="KEGG" id="pgr:PGTG_04865"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04865"/>
    <dbReference type="eggNOG" id="KOG1494">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047181_1_0_1"/>
    <dbReference type="InParanoid" id="E3K352"/>
    <dbReference type="OMA" id="HINTPSI"/>
    <dbReference type="OrthoDB" id="4069699at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01337">
        <property type="entry name" value="MDH_glyoxysomal_mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000017">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.110.10:FF:000001">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001252">
        <property type="entry name" value="Malate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010097">
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        <location>
            <begin position="10"/>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:57540"/>
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            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
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            <position position="85"/>
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        <ligand>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
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        <location>
            <begin position="121"/>
            <end position="123"/>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:57540"/>
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        <ligand>
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        <ligand>
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            <dbReference type="ChEBI" id="CHEBI:57540"/>
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        <source>
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    </evidence>
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        </source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EFP78909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="332" mass="34988" checksum="E944BAEB6F9B8B59" modified="2011-01-11" version="1">MPSLKVAVLGAAGGIGQPLSLLLKQNPHITELALFDVVPVVKGVAVDISHINTPSTVTGHIPAEEGLAKALKGSDLVVIPAGVPRKPGMTRDDLFKINAGIVRDLATSMAQNCPKACILVISNPVNSTVPIVAEVFKKAGVFDPKKLFGVTTLDVVRASTFVAHVVGQPEKAHEYKIPVIGGHSGVTILPLLSQSKPPLPQSVLSDKSKVEELIKRIQFGGDEVVAAKDGAGSATLSMAYAGFRFAESLIKARLGHTGVVEMGYIYVADDKHISAHTDGLEYFSVPIELGAEGVGKLLPIGDINDHEKEMLKACVSELKESITKGSSFVNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3J1</accession>
    <name>E3K3J1_PUCGT</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
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    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_05004</name>
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    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP79048.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="444"/>
            <end position="460"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP79048.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="480" mass="54276" checksum="6756BFB30ACF13AC" modified="2012-04-18" version="2">MMKDHQRRFTDDNPWNSVPPGVNPFQFSIDRSAHQGFAPLSRLAKALLLVFFVFHLLIAIFCLIILVLPYIRGVKQSHWFFRKLYIQDQSRQKGLFELSLPLPLDCFNSYECFSSFALQVYKVPLLWVNGGVVMTMSQLMGSVATLAYILIQLAITQSINASLHVQVEPALAMMCMCEMLTYWSQMHCFVVAMFYHNHKTDSNGPKRWTPPPIFINCLFLGFPMVLVIATLPPVIGLSLNHNQITIGVTSIMNTLRQGASVWDQLKVPSTTIEEKHELTTQLNQIMSQLKDLGVHIGIHAERLKFFIVVFGVLVQKVQQAEEQSACGSSASKPFRRWFNLKNHATTSGVEPKDRSDTGEQTSIMNRQFFHLILRSICIIVAMCTSITLCILGIVKSHDGSIRSSYWAGVTTWLATLAGSWSAIPIVWQCWRLYNDDLGRTFQSSVNSRHAPSSGESNLKNSVHRKPSEERLDIRIELAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3Q9</accession>
    <name>E3K3Q9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04667</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78711.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323130.2">
        <property type="nucleotide sequence ID" value="XM_003323082.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3Q9"/>
    <dbReference type="STRING" id="418459.E3K3Q9"/>
    <dbReference type="GeneID" id="10536648"/>
    <dbReference type="KEGG" id="pgr:PGTG_04667"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04667"/>
    <dbReference type="HOGENOM" id="CLU_2198250_0_0_1"/>
    <dbReference type="InParanoid" id="E3K3Q9"/>
    <dbReference type="OrthoDB" id="10471360at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78711.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="108" mass="12382" checksum="3E4AA650A22417E9" modified="2012-04-18" version="2">MTYKPSPSDLVQMPALGSDWTKDKSKRDKDWEGKDTLIDKTKWLDRKQKIRSKKQVGKFEFNRKINLAMTAKSSSVQPKTSIITFIIKDLSSAGNPVNRRQRLELGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3V4</accession>
    <name>E3K3V4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04712</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323175.1">
        <property type="nucleotide sequence ID" value="XM_003323127.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3V4"/>
    <dbReference type="GeneID" id="10536774"/>
    <dbReference type="KEGG" id="pgr:PGTG_04712"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04712"/>
    <dbReference type="HOGENOM" id="CLU_044243_2_1_1"/>
    <dbReference type="InParanoid" id="E3K3V4"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="127"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="179" mass="20584" checksum="A0F55683F8BC2A83" modified="2011-01-11" version="1">MTTLTRKEPTTSQDKETTRGELPQNMEKTSDMEIDEIDPECSEATPIRPSTPEIKVLSKEDQVKLRVKEHVKLWKQCLVASREGPTARLRALLTTAQESQKTLQKLIDNEEIESYVKGWNPWEEKKIHFPSPPKKNLMKFKRNEPGKKQSSSSTNFSDPAKWKKVTDLLQMASGLYQNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3K6K4</accession>
    <name>E3K6K4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_05126</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EFP79901.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324320.2">
        <property type="nucleotide sequence ID" value="XM_003324272.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K6K4"/>
    <dbReference type="GeneID" id="10533592"/>
    <dbReference type="KEGG" id="pgr:PGTG_05126"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05126"/>
    <dbReference type="eggNOG" id="ENOG502S2AH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011407_4_0_1"/>
    <dbReference type="InParanoid" id="E3K6K4"/>
    <dbReference type="OrthoDB" id="3253684at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041320">
        <property type="entry name" value="CxC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040521">
        <property type="entry name" value="KDZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18802">
        <property type="entry name" value="CxC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18758">
        <property type="entry name" value="KDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxC1-like cysteine cluster associated with KDZ transposases" evidence="2">
        <location>
            <begin position="141"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="951"/>
            <end position="980"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="951"/>
            <end position="961"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP79901.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="980" mass="112775" checksum="A5B12F4F86CEDB32" modified="2012-04-18" version="2">MAPGKRPKNLYQFHYRSQLPNTETRSEKKARLACQKRTKAEIGDSIRRQINRRDTQDQNDQESTNPDNPDTETHAQESLGFNENSLWEDTGEYMNDEDSNSLARIRSLHEEFIQKQKHQHWKDVMDSMFPVYLHLKNQTADWTLPCALDNFSSLLCKCTPDRYKIREVDLIDLMGQKREKFRFCPCTPDPVLLLANGYLSCTPIVPQTAFSIRLLNFYDLLWNFCNTHATPFCKVLQTWNKAISVRLFAKNSKRPQDLRRNLVGSINAYQTLKSMQRNLVQTVTSITKQEVLAQQSCPACFGVALPNDPTFNPDSHSTQPSDTNKIFICLDGNFQHRHHERASKNYLGLKNEPLFVTPEEIALSNVEIREGEIAKRVSQKAKDRCTQQHKAADDRQNASSWKGCDDTGLFGCCCRHDSVISFCNIHKTGEGRGLPMSIIKKLFSEINPDIKLGILYDIGCTLKKFFQARGLLSNYLPRMLFATAVFHSYVHDWPCQLQFNPRYNDGWGLTDGEGLERLWSYLSPLVSPLRYATRNHRITAINHRILTLKRKFIHAIATKIESQKIVQRMLKDQNPHKPGQCFTEDFFRAEWKKQRDFEINRNETDRLKKEEQAQFFERGEALKSLVESFVASLASSSAHSDPTHALSMLEEIRDLQQKQDEEIERLGSHFAVDTGAERNREQEKRLALLWSAKSALYKYAVQIQGEMQPLRDSKSHGERLGTVLKEKIFKALGRRKNTVTRVLKTFCDRRTDYLKNHAPNQLSLPENKAITYDDFTKLKLDDPFWNDAYLCLSKDPWAVDPTVRTGIHALLRLDRAKEELIQLNNELRRCLSWGIQHRKQIKLRIDQCVFDTVDQHLKAVLEDSFGQVSYQTRKILSDELELVQRKHKHLLLSWQPAVQEILALGTVSRSALPAEWFALVEFLKQSDAVNGLETADVDLLLEETILNLQDSDGESANDDINEGAIGVEHVPPEDDDGELS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3K735</accession>
    <name>E3K735_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Alpha-amylase</fullName>
            <ecNumber evidence="4 9">3.2.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_05203</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Endohydrolysis of (1-&gt;4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1-&gt;4)-alpha-linked D-glucose units.</text>
            <dbReference type="EC" id="3.2.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the glycosyl hydrolase 13 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.1" evidence="4 9"/>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EFP79978.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324397.2">
        <property type="nucleotide sequence ID" value="XM_003324349.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K735"/>
    <dbReference type="STRING" id="418459.E3K735"/>
    <dbReference type="GeneID" id="10533832"/>
    <dbReference type="KEGG" id="pgr:PGTG_05203"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05203"/>
    <dbReference type="eggNOG" id="KOG2212">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013336_0_1_1"/>
    <dbReference type="InParanoid" id="E3K735"/>
    <dbReference type="OrthoDB" id="550577at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004556">
        <property type="term" value="F:alpha-amylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043169">
        <property type="term" value="F:cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11317">
        <property type="entry name" value="AmyAc_bac_euk_AmyA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006046">
        <property type="entry name" value="Alpha_amylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006047">
        <property type="entry name" value="GH13_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43447">
        <property type="entry name" value="ALPHA-AMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00128">
        <property type="entry name" value="Alpha-amylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00110">
        <property type="entry name" value="ALPHAAMYLASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00642">
        <property type="entry name" value="Aamy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173587" description="Alpha-amylase" evidence="10">
        <location>
            <begin position="24"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 13 catalytic" evidence="11">
        <location>
            <begin position="79"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP79978.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="524" mass="58705" checksum="71ACE1D403097BD8" modified="2012-04-18" version="2">MQLPKRHKSSWVSLLALATLACSAKSPASLGSLTQNHLLFPLAGLKNWLVRRRSHRVDEQLVASCTRKRLDDDPPSTDRVTVQLFQWRYSDVGDECEAFLGPNGYRYVQVSPPHEHIEGPQWWTDYQPVSYRIGSKRGSEAEFDRMVQRCGGAGVGVIVDVVLNHMSAGSTTEYRRGSSGSSYRKYEYPDLYTRNNFHKCRKRGNLEITNYMNRYEVQNCELVGLADLKTSQRHVQVQLKSYLTRLVDKGVAGFRIDAAKHIPASELLDILKLIPIRVVQEVMYTGGEPIRPEEYSAIGRVHIFQAAYDLKRMFLSDGIAYLGHPVPWGPQWGDGYLPSSHSQIFVTNWDLERNEHGIRIDKDPGAYLLCQVFILTWGYGQVDVFSGYHFSAYDDGPQDPVAQCGRFGWRCEHRHPMVVGAIQLGAAASDQPVHNIITSGAHRLAFSRGSKVFVAINNDDSAWVLRDVVVGLPLPASPEDAYRDRLHPAGEGQLVHLQPDPSSSLARLCPLTIAPRSAIGIMVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3K8C8</accession>
    <name>E3K8C8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_06694</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80738.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325157.2">
        <property type="nucleotide sequence ID" value="XM_003325109.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K8C8"/>
    <dbReference type="FunCoup" id="E3K8C8">
        <property type="interactions" value="73"/>
    </dbReference>
    <dbReference type="GeneID" id="10539757"/>
    <dbReference type="KEGG" id="pgr:PGTG_06694"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06694"/>
    <dbReference type="eggNOG" id="KOG0255">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008455_11_4_1"/>
    <dbReference type="InParanoid" id="E3K8C8"/>
    <dbReference type="OrthoDB" id="9986881at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17323">
        <property type="entry name" value="MFS_Tpo1_MDR_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000082">
        <property type="entry name" value="MFS multidrug transporter, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23502:SF74">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="81"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="152"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="175"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="212"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="240"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="313"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="357"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="397"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="425"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="453"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="488"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="80"/>
            <end position="515"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP80738.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="551" mass="60097" checksum="D11DEB64A68FCBB9" modified="2012-04-18" version="2">MDGELWRKDVDMGKYGGDPLASVDVGCTTIEESLNIPSASPPSTIVSPSPADSPANGLPEGAVLVVWAPGDPDNPQNWSIAWKWTITIFCSVLTLNVTIASSGPSSATEAIMSEFQVSEVVATLVTSLFLCGYVAGPIIWAPLSEMIGRRDVFIGTMGFFFILQVPIAAPDSNLATIMTFRFLTGLFASAPVSNSGGVVADIWDAVHRNDAISLFTASVFLGPVIGPVVASFIVESSLGYRWIFWVLMIASGATWLGSLFLLPETYSPILLSRKAKKLRKETGDSKYYSAHEKSDYSVKAIFRRTILRPFEMLFTESILLFVTVYLALVYGLLYGLFEAFPVIWGEIRGFTPWQTSLVFIGVGIGSLLGALLNIYLARPMVRVVEKWHGHPPCEMNLYGAMASGPLLVIGILWLGWTGAYAAVPWWVPALPTVLLGITFTSVFISFQSYLVDVYLMYAASALAATTIFRSAVGAAFPLFMRQMLHGMGTQWACTLIGCFSLVLLPIPFIFYKHGAKLRVGSHFAPALDVKMRARVAEEEAEENSKTIEKQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>E3K8N7</accession>
    <name>E3K8N7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_06574</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80618.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325037.2">
        <property type="nucleotide sequence ID" value="XM_003324989.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K8N7"/>
    <dbReference type="FunCoup" id="E3K8N7">
        <property type="interactions" value="2"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K8N7"/>
    <dbReference type="GeneID" id="10539632"/>
    <dbReference type="KEGG" id="pgr:PGTG_06574"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06574"/>
    <dbReference type="eggNOG" id="KOG0998">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006395_0_0_1"/>
    <dbReference type="InParanoid" id="E3K8N7"/>
    <dbReference type="OrthoDB" id="524326at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016197">
        <property type="term" value="P:endosomal transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00052">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000261">
        <property type="entry name" value="EH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015940">
        <property type="entry name" value="UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216:SF170">
        <property type="entry name" value="DYNAMIN ASSOCIATED PROTEIN 160, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11216">
        <property type="entry name" value="EH DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12763">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00627">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00027">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00165">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50031">
        <property type="entry name" value="EH"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50030">
        <property type="entry name" value="UBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EH" evidence="4">
        <location>
            <begin position="12"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="45"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="4">
        <location>
            <begin position="142"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="domain" description="EH" evidence="4">
        <location>
            <begin position="308"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="5">
        <location>
            <begin position="341"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="domain" description="UBA" evidence="3">
        <location>
            <begin position="1127"/>
            <end position="1167"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="109"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="247"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="389"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="674"/>
            <end position="746"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="770"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="973"/>
            <end position="1134"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="571"/>
            <end position="633"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="109"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="409"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="425"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="442"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="468"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="725"/>
            <end position="736"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="773"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="813"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="828"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="871"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="923"/>
            <end position="942"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="983"/>
            <end position="996"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1001"/>
            <end position="1010"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1023"/>
            <end position="1032"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1051"/>
            <end position="1064"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1076"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1092"/>
            <end position="1106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1123"/>
            <end position="1132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP80618.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1167" mass="124074" checksum="5D7CC8BC061206F1" modified="2012-04-18" version="2">MAPTALQLSSEERLAYPYLFSKADTDQIGVLVGEKAVAFFAHSNLPPTILGEIWQLADQDNAGFLTRQQFDIALRLIGKAQRGLPVNEQAISSPGPLCVLKGFSVPGLPSPSTPPAPRPPNSTPLGISEPVQDPLYTIGNEDKLKYSSMFIKAGPRDGLLDGDKARDIFIRSKLSFEKLGQIWTLADTQSRGALSVSDFCIAMHLIQLSMSGRLSTFPTSLPSALLDSATAPVAGAVLPISRQVTGQQQSIPGSLPPSSPLRKSSTGSAIRPQYTGRSASGVPSSLPTGINSIIGSPTGLAWDISSAELAQSNIYFDSLDTNRLGYITGDRAVPFMMESKLPGEILARIWDLADIRGEGKLNREEFAVAMRLIQDTLAGGVESLTAKLSPGMVPPSLREPAGLPAIPETSTTQQDLLSLLDDPPNVTQPSQNDANKPKHSAPPNQQPSLPVQLTGTHSMTPAARVASPLSSSQGYIGAQTTGQFGQSSSSRGLGEDDGAKLGNLVNQLSSTENALSTLHDERSRLERHTRSTVEQIKTLELKLSTARQMHQNELPLVEELRRKQVEQRSTMSKLTSDVITAESELSSLKLEKEQIEGSILRDKEEIRDMKKRMAQLEEETQRLKSSLETLKKDSRLQKGLIAIGKKQVLTVEGQKADISKEMDDLTQQIHSIALPPEGSRAPSPGVPPQSVPSQKSTNPFERMMPAASLTPQASAIHSPEASGSRDLNQSPHSSATRLGVSTDPFLGGSDGPAQFLTALSPNTLSFGDAFGLDESKTEDNRSPADHGGFHDARETTSPDPPPSAKFSPPIAQRSEETKKTEDPKKDESDESSDEEEIEDPVARHLAPSSRPGKAASFASDKFPALEDVEASVQSKATASSPVTSQDTGLQGGKGESSMTQAGTSLGTGAGGATPGSTIKASPIKSSATSNKSVEQNKPSINSGDELEVSDFVADFGLIGSSSSAAGATLEDFDSAFENLPSPESKRNPTRNGEHKPGNPHLTGTTTTNSIGFDDGFDDAFVVMDRESSKKDQSSPLGFDESFSDFDKILAGGNSSQNTSSGDSSKVQKKEQDDPFGSPPPSSPTEPPPLPTRPTNSSSLTSTTSTSAAGPAQQSQQDMSAKNEPAKPTDSDAVKQICGMGFDRKVVIQALERDEWDVDRALNHLLGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>E3K9E2</accession>
    <name>E3K9E2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_07133</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP80881.1"/>
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        <property type="nucleotide sequence ID" value="XM_003325252.2"/>
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    <dbReference type="FunCoup" id="E3K9E2">
        <property type="interactions" value="59"/>
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    <dbReference type="GeneID" id="10539905"/>
    <dbReference type="KEGG" id="pgr:PGTG_07133"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07133"/>
    <dbReference type="eggNOG" id="KOG0111">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_27_1_1"/>
    <dbReference type="InParanoid" id="E3K9E2"/>
    <dbReference type="OMA" id="YKPVWID"/>
    <dbReference type="OrthoDB" id="407442at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005654">
        <property type="term" value="C:nucleoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48037">
        <property type="entry name" value="ATPASE E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48037:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="2">
        <location>
            <begin position="9"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP80881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="141" mass="15772" checksum="026194B7AAC418D6" modified="2011-01-11" version="1">MPKEASDNKTIFVGNIPPNINAEALHSSFSPFGNIRDIQIPTDPQSRNNRNFAFITFQDQIEALDAIDNMHLNLLSSDSEKILKVNLARNSFHNNNQKADAKGFSNPNNRAIWDDETWIKENALKEDSKGDPVVQDANPAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3K9K8</accession>
    <name>E3K9K8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_07159</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP80907.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325326.2">
        <property type="nucleotide sequence ID" value="XM_003325278.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K9K8"/>
    <dbReference type="GeneID" id="10536065"/>
    <dbReference type="KEGG" id="pgr:PGTG_07159"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07159"/>
    <dbReference type="HOGENOM" id="CLU_491867_0_0_1"/>
    <dbReference type="InParanoid" id="E3K9K8"/>
    <dbReference type="OrthoDB" id="2504686at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="147"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="392"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="471"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="509"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="23"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP80907.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="554" mass="62657" checksum="6F0CC13E83B0B888" modified="2012-04-18" version="2">MARNAHGRNRHNTTADFLITGTSRRSRTQAAQIRNQARELEEHRAFLEEVSIAQQLGIVDASYAQQLAGPPHEDDEQERSQFEPLIGSYVGEDFENDPSGGTPPQNMIKFVSSLPPSTNPYVALAGKIRDQTRIAKPVLWGIIVRRIITFQFCVLCAQAITVLWLRKKANKLRFFRCNKLGLIHVEVLNEIVLLMLVFSVLAVIDLVTQEFVEQGSMVFSNKLILRTCKFPIAADVCWCMSSSQLDLPLPSKRVSLMKPVSGMNPIDFRSVSVDLRDRECDVILLFKSTGTRRSIHQTSGVHPFQRQWLTPRRSHRSWGEALLLGTSIQGTLSIRGIEQAAREIIETLLRAAPSYRPETFNYLSMFGLILEPGQRILSGTKRFAHEVRLIQILYIVQHLLIATLYMPTSIFALRGLRNQVTPECALKRGPAEEEESLPNYQLQIMTLTMKTPGQEMANQEKVRKRLIKQTLMLYLDTILYCPPLMYMLSYKGVSFFNDPTWVLIEQLGTHGHTAIIGNIILALLIKNAIYTNKRAATSTLAPPDIPYLMRLSGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>E3K9U6</accession>
    <name>E3K9U6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">UmuC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_07406</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA polymerase type-Y family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP81154.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325573.2">
        <property type="nucleotide sequence ID" value="XM_003325525.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K9U6"/>
    <dbReference type="FunCoup" id="E3K9U6">
        <property type="interactions" value="373"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K9U6"/>
    <dbReference type="GeneID" id="10536558"/>
    <dbReference type="KEGG" id="pgr:PGTG_07406"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07406"/>
    <dbReference type="eggNOG" id="KOG2093">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_330134_0_0_1"/>
    <dbReference type="InParanoid" id="E3K9U6"/>
    <dbReference type="OrthoDB" id="2505578at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.100">
        <property type="entry name" value="DNA polymerase, Y-family, little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036775">
        <property type="entry name" value="DNA_pol_Y-fam_lit_finger_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017961">
        <property type="entry name" value="DNA_pol_Y-fam_little_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053848">
        <property type="entry name" value="IMS_HHH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001126">
        <property type="entry name" value="UmuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45990">
        <property type="entry name" value="DNA REPAIR PROTEIN REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45990:SF1">
        <property type="entry name" value="DNA REPAIR PROTEIN REV1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00817">
        <property type="entry name" value="IMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11799">
        <property type="entry name" value="IMS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21999">
        <property type="entry name" value="IMS_HHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100879">
        <property type="entry name" value="Lesion bypass DNA polymerase (Y-family), little finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50173">
        <property type="entry name" value="UMUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0237">DNA synthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UmuC" evidence="4">
        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="376"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="537"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="619"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="380"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="555"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="619"/>
            <end position="629"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP81154.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="869" mass="97496" checksum="72002D3C802C84CB" modified="2012-04-18" version="2">MHVDFDCFFISAGLIARPELKGKPLAVCHAKATNSAKPNGSTSEIASCSYEARAFGVRNGQTLGRAKELCPEIQTIAYDFKLYEDLSFKFYNILLSYADELQAVSVDECLIDVSSRFINQYGPDGFNSIESSALKLAEEIRTTVRSATQCEVSIGISYNVLLAKLATKKAKPCGAFFLDTGLAQAILKDLSIDDLPGFGWAQKNEIDNKLSVDNVGDLQTFPLSRLVEVLGPSRGKTLYQYAHGLDDRTLKSSNHERKSVSAVINYGIRFKKLDEGGNDDVRIFLRQLGQEVSRRLTELGVSGLQLTLKIMKRAPKCTESSKTTKLSGPGGVGVSDGTIIGNEAWKLLQSMNIPPEDLRGIGIQINRLEKLSKNIHSDKPQGQSTLMFQTTKPMEPPARRQDSRRQSIHSSNKIITNHGDRTPVASTSKKIFKTPEALNSTPFAIPPASQLDLEVVEALPTPLKSHILKSIACHQKPPNTSKLDNHQDTIDLVTPDSKMRENPNQFDLPSVSQVDWKMLAELPSSVRKPIEAIYAQRQSRRQLPPPTPPNFTRGLKGHRKEVNHRIPRSSQKKDRLASLHLAFQDQRVSRAKSTIKHRVAPLGPSQKLINKKTFKLITRNQPAKTPSKNEQGDAEEEATELLPTPNKISDEQLEALEIDVRFFRELKGNSTRAFQLDLIWNQFQIHSSRFKEVTRRTDRWKKWRKNFDRPAKVLQICLPEPPCLASRGGARGRKGLSKLEDVLELIEQWMLSSTDLPTHPVGISGKQPSTISLVEIDQRDLEIIQKFLIDLIDPPPPPPPKLSPLDGQQQSSLGQDFEKVFKIIQWWDHLILKLFAHNHPAFIYWKNILDNLKLKINSIACRDFGDPIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KA96</accession>
    <name>E3KA96_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06874</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81253.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325672.2">
        <property type="nucleotide sequence ID" value="XM_003325624.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KA96"/>
    <dbReference type="GeneID" id="10536740"/>
    <dbReference type="KEGG" id="pgr:PGTG_06874"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06874"/>
    <dbReference type="HOGENOM" id="CLU_086121_0_0_1"/>
    <dbReference type="InParanoid" id="E3KA96"/>
    <dbReference type="OrthoDB" id="2508185at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172499" description="Secreted protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81253.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="225" mass="24870" checksum="1E96CAB36E390610" modified="2012-04-18" version="2">MKATTSLFFLAAVAMSAVMADFDTTPPHTTCYNHFLTTGKKCVYSSPNDMERCPAPPDPPKTSQSVPLFQHQISMNKRSEDHTLGRRYDTTKPVTTAVKMVFTCPEPQNPDPNDDSSGLGVCLWVGANCNSTGWVNKDNFSNCGKQLYIQRKGDIMNPKYPRVIGGCDFGPDLPNEVGCFNLAVNQKLFDAFNPTEEERSTGVLSNIMTWDFNNLKGSKKQNGPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAR2</accession>
    <name>E3KAR2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_06802</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325600.1">
        <property type="nucleotide sequence ID" value="XM_003325552.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KAR2"/>
    <dbReference type="GeneID" id="10536594"/>
    <dbReference type="KEGG" id="pgr:PGTG_06802"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06802"/>
    <dbReference type="HOGENOM" id="CLU_025212_0_0_1"/>
    <dbReference type="InParanoid" id="E3KAR2"/>
    <dbReference type="OrthoDB" id="2506374at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033018">
        <property type="term" value="C:sarcoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051279">
        <property type="term" value="P:regulation of release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP81181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="264" mass="30020" checksum="B09E84AC26DDDD29" modified="2011-01-11" version="1">MELSGIEKEVFSPPIFQFFSKIPCSYDNDDEDEDELAEITLTQEEEELYRPLYDRLVNHEKVKMVVCEGPISQNKTSTALKQAESNLQRLNSEIFTVSKFYNSTYYLLISSRVPGAHSFCHEYSNDAGWLAITASRWTLKAQFEAYSQARELQEVLEKSVGVSIVKKKKKPSDELKEKLREALNTTLASARGLPVKSKECVFPKTKDPAAGLKKLDSRLEIVRSEQSTLSLELLKLGFEGLKLEQKRLWLADIQSGAFKVQLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3KB71</accession>
    <name>E3KB71_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Superoxide dismutase copper/zinc binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_07833</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81584.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326003.2">
        <property type="nucleotide sequence ID" value="XM_003325955.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KB71"/>
    <dbReference type="STRING" id="418459.E3KB71"/>
    <dbReference type="GeneID" id="10532965"/>
    <dbReference type="KEGG" id="pgr:PGTG_07833"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07833"/>
    <dbReference type="HOGENOM" id="CLU_116404_0_0_1"/>
    <dbReference type="InParanoid" id="E3KB71"/>
    <dbReference type="OrthoDB" id="159229at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.200:FF:000015">
        <property type="entry name" value="Copper/zinc superoxide dismutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024134">
        <property type="entry name" value="SOD_Cu/Zn_/chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001424">
        <property type="entry name" value="SOD_Cu_Zn_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10003">
        <property type="entry name" value="SUPEROXIDE DISMUTASE CU-ZN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00080">
        <property type="entry name" value="Sod_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171877" description="Superoxide dismutase copper/zinc binding domain-containing protein" evidence="1">
        <location>
            <begin position="29"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="domain" description="Superoxide dismutase copper/zinc binding" evidence="2">
        <location>
            <begin position="61"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP81584.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="191" mass="20242" checksum="8B86F3BD54CEDCFD" modified="2012-04-18" version="2">MNLRIPNSANFMGLLLFGIAFVPDILRAQPPLPPTKPPGRPPTRFPCTEASVLINGLQGITGTAFFKPNVGRNRGIDFKISLNGLQRVENHKYHVHEFALTPGGGCKSAGKNFNPTKKPIPCPANSPQSACAIGDLSAKGGSLKPATAGATVGTEYLDTVLKFSDIQGRSLVIHDQGGDYIACGENSFKLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBE5</accession>
    <name>E3KBE5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF1740-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_07907</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NRDE2 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81658.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326077.2">
        <property type="nucleotide sequence ID" value="XM_003326029.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KBE5"/>
    <dbReference type="FunCoup" id="E3KBE5">
        <property type="interactions" value="268"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KBE5"/>
    <dbReference type="GeneID" id="10533203"/>
    <dbReference type="KEGG" id="pgr:PGTG_07907"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07907"/>
    <dbReference type="eggNOG" id="KOG1972">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007550_1_0_1"/>
    <dbReference type="InParanoid" id="E3KBE5"/>
    <dbReference type="OrthoDB" id="297219at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902369">
        <property type="term" value="P:negative regulation of RNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031048">
        <property type="term" value="P:regulatory ncRNA-mediated heterochromatin formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003107">
        <property type="entry name" value="HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013633">
        <property type="entry name" value="NRDE-2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13471:SF0">
        <property type="entry name" value="NUCLEAR EXOSOME REGULATOR NRDE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13471">
        <property type="entry name" value="TETRATRICOPEPTIDE-LIKE HELICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08424">
        <property type="entry name" value="NRDE-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00386">
        <property type="entry name" value="HAT"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="307"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="75"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="117"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="308"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP81658.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1213" mass="139014" checksum="98684216B1004335" modified="2012-04-18" version="2">MTTVPSFDSFPSPTHSPSENKPVVAIPSFDSFPEPEAVTKNRRKRRTNEDNEDSSRSSSTRRRTKQRITTDHSTLDQADQFLNSLSHQIDSAHQRNSQESPDHQAQIQGSNRRRKERRSDHQSRQDSPVRKDKKAKKKLQAITPPALRALPGLSATSTIQPKLDSNQFRDQLGKIIFIIDLREDNFNLHHGRPDRGKVPRYRRAAAGRVLGLDPHLRIVFSSAYRGDGLKLSTEGRTKPVSISDPAVLQLLNDKNLKRISLSRLPHGGGEKMGDGPSIPFIPLLESDRPAPGVEQERIPVGINLYGEPESEEEDLEDYVPPEEDGGESFLDRLNHRKGELERRTTDHPEDVEAWLALVSLQDEMKELGLVGLRARVTDADQEAISRHIQGTSDLKIAYLKRALREPANQNNEQLLLAYMKAYCDQPDLDLAKEWQKMLEAHPAVTGLWIAYVDWKQTEAGSMNVLGMIEVYEELIDRLVRRSENSNTPDKERTLFEQSIVYLFHRCCVMLKQAGYNERATAAFQAIMELNFFKPRQKVESLNELADDLETFWDSEVPRIGEDGAKGWMNMNLEFEQLEPQPGQTLPNESKELEPNPTEEIFNRWQVQEMKARRPLRTTDLQDNDDPFGCVLFDDIRNLLFLLSTDDSLQALLYSFLSFVGVSIPPPDIDTNVPFFTDPFLSTELIDVPERQSTFWPQLEKKLSFDPYGPTERSSGIKNPQMIPFRIFPCDIRQLFADPSRWFVNFEPPKDSINFIRNSFQALKTTGMLKQDTYFKLCELSFEARVDVSSAVKLAKQSLSLDQRNFVLWDAYARLCLLQGKPKQARDVYVKTLEMSEQGAEGLMSLWYSWAEMEFQLGHIRLVISILARTVNCLDESPSGNSLLRLAQPFHCYQKTALADLGFRNARARPQYFPVQVSRLVASCSTRSFQDDLPLSLLRCRVSAAVGYGTFQYLTEGFPAGCEAYERSLKALESLDSPAEEEELWLNYCRLIYQQIQDHQRFKPIEIRATLQRAIVKFPNNSVFLALFAFNESKMKIENQTRRLLDAILLKIPTLVTVNRWLFAIWVELHLNSSTYNQAAVRGLFERALTNQSIGSCLQVWVLYIEFELRNENYVRARSLVTRSVAHCPWAKELYLLPFTSRFAGAYETSAGRAVELNRMVRLVDEKCVRIRDRSVLARLQNAEVDRLPRVLGEEDLDSGEQREFDERTKLMPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCW3</accession>
    <name>E3KCW3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_07646</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82249.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326668.2">
        <property type="nucleotide sequence ID" value="XM_003326620.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCW3"/>
    <dbReference type="GeneID" id="10529622"/>
    <dbReference type="KEGG" id="pgr:PGTG_07646"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07646"/>
    <dbReference type="eggNOG" id="ENOG502T0GQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054434_0_1_1"/>
    <dbReference type="InParanoid" id="E3KCW3"/>
    <dbReference type="OrthoDB" id="8026949at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="3">
        <location>
            <begin position="277"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP82249.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="322" mass="35769" checksum="670511C937096F09" modified="2012-04-18" version="2">MAGWKETLLAGEVLVPCRLEGCPSSPCTSSTGRASFQPARYLYLVDWKETLPAVERVRMRIRMLRWPEKAAGYPDANRPSLIVTGKLLRPETDYKDKDSKPVTQGSHRLYYAEESASDWDVLLDIARATLKMTLSVDLAMRYKDTKPVSLLFKTICDAYEKNTRARRMMLQDAFWTACHDPSNPIATWIARVRNLASDLKSVRLTPADQQICDRLLRGLDKSWKPIRDHLVYSPNEISLDDAIGALEAHEVSMQAPFDQPQDTFAALAVARKKKPGCWNCGEFGHHSSLCPNLSVKNKSKAKFGTRAGAASAVALGGGGPSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KEJ1</accession>
    <name>E3KEJ1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_08754</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326977.1">
        <property type="nucleotide sequence ID" value="XM_003326929.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KEJ1"/>
    <dbReference type="GeneID" id="10529945"/>
    <dbReference type="KEGG" id="pgr:PGTG_08754"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08754"/>
    <dbReference type="HOGENOM" id="CLU_1907708_0_0_1"/>
    <dbReference type="InParanoid" id="E3KEJ1"/>
    <dbReference type="OrthoDB" id="2502132at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP82558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="133" mass="15323" checksum="4886CCCB7E98A9E8" modified="2011-01-11" version="1">MTYLDLFLNAHTQLTELLKQKIDEFSRATARREEKKVTISEFQNIVDGIKTILAGSMSAKVHRLCDLLSDSIYLDKIDTPRLQDGIKVLKELEESVEQLSTLIALLRVPNHYQFFRNYEGGLVYLVFLLMIST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3KFF7</accession>
    <name>E3KFF7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09952</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178284">
        <property type="protein sequence ID" value="EFP82984.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327403.2">
        <property type="nucleotide sequence ID" value="XM_003327355.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KFF7"/>
    <dbReference type="GeneID" id="10544695"/>
    <dbReference type="KEGG" id="pgr:PGTG_09952"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09952"/>
    <dbReference type="HOGENOM" id="CLU_032241_1_0_1"/>
    <dbReference type="InParanoid" id="E3KFF7"/>
    <dbReference type="OrthoDB" id="2507256at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="200"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="236"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="309"/>
            <end position="318"/>
        </location>
    </feature>
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        <location>
            <begin position="319"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="341"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82984.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="413" mass="45937" checksum="641CC909E02C5EF9" modified="2012-04-18" version="2">MLSFQAVDPALLPALLAPASSVSSLNPTSSVPPLNTIPSNTTTKKKRTRRTKKQMALARDNDITNNGETAKTDNNPQFIHSDYENICAYVEDDEKFSELYGDTKTNVGPKVLTKTAAFNIFAIHINAHSNKRLNLTGRQLQQRVNYYIQKKYFPAKQWENQTGAGILDQDPHASLDEALEAKCPCYAKMDAIFGQRPNITPLAEFDSSNPTPIGPSQFEHQDQDSSPEVFYPGWDVSQSPPANQSPHLDADVRDETLQLMIPPLLCDGEDSDALPQSTLASFPALPVGANPTPGGVQFTPTPTAPCRTFPNQANSNETRPPKERVDKSKSSLATAYQSDKQSNEDRRFNWEKQQYDDERADVQKRLEAEEKMQQARLDAEEKKQSAQIEAAKRWISQGKSTTEVDVLLKAVFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGD0</accession>
    <name>E3KGD0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08541</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003327726.2"/>
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    <dbReference type="GeneID" id="10545071"/>
    <dbReference type="KEGG" id="pgr:PGTG_08541"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08541"/>
    <dbReference type="HOGENOM" id="CLU_717933_0_0_1"/>
    <dbReference type="InParanoid" id="E3KGD0"/>
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    <dbReference type="OrthoDB" id="2504060at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <end position="155"/>
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        </location>
    </feature>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="390" mass="43703" checksum="6762BD8D80DDE6EC" modified="2011-01-11" version="1">MRLFTENITASISRRQRPSIYSETLKSSETTTTTTTTNNKQAQQLPRTPFKNISRNSSSTSPTQSTHTNNNNNNNNNNPLNLASSAASSYSASFPHNPSFLTIPHSQATPHHHPNPSSVRSESVPRKSSACSSTRSTIENEPSRSHGRHTPLPGFRSASALSQISFRSLRSINSRIKVRIQSLGQSLEARKPKLPELDWSALRAEYELHLARGSIIERCLVQVDLGPSVTERGRWWIDTVWTLADKLSPPLPAALAQYSASGLIPLLTPLRHTLHRLAVELPYNHLEDTPDLDKFEELGRILRRAIWLAGLIENVIELVSQVGLVRFELAGLRRPFEDLKLNDDDELKFDDSLQLDQLLDRSIRLISHWNGEIRGRTGSNNPWKGSIKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHF1</accession>
    <name>E3KHF1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_09439</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83726.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328145.2">
        <property type="nucleotide sequence ID" value="XM_003328097.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHF1"/>
    <dbReference type="GeneID" id="10532413"/>
    <dbReference type="KEGG" id="pgr:PGTG_09439"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09439"/>
    <dbReference type="eggNOG" id="ENOG502QQGC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010748_0_1_1"/>
    <dbReference type="InParanoid" id="E3KHF1"/>
    <dbReference type="OrthoDB" id="65716at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056751">
        <property type="entry name" value="PAS_13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053045">
        <property type="entry name" value="Zinc_cluster_trans_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31986">
        <property type="entry name" value="REGULATOR OF DRUG SENSITIVITY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31986:SF7">
        <property type="entry name" value="REGULATOR OF DRUG SENSITIVITY 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24990">
        <property type="entry name" value="PAS_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="9">
        <location>
            <begin position="70"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="119"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="537"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="44"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="119"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="163"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="537"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="567"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="579"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="600"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP83726.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="664" mass="74427" checksum="91A99491710A6A1A" modified="2012-04-18" version="2">MSSSTSHHQSSQQLNQLHQHQQQQQNDNQQQTTNQFNLPPQQTSDKPQLNQQQANKTTKPITKRRKVAKACLFCKRSHMTCDDSRPCRRCIKREIGHLCCDEPPSASERSNLLATIKNHSQPTQTQQPSQNQSASASASHHQQQQQQQQPHPTSQNQFKKQTDQQPSISKSSLIDPSISAPNQLNPLTPAIDSLNNPINQLSHFTTLFSPRLLLSPSSTDSPLTNPLDQSLAFFRGSSGLKLFEEPAESTHDDPEQYLKNGLLEDSKGTPDSKAEGSMIDLSEFLNTYPGLFSENIGNLNQSLHRSSATNQELLETTGISPAAINYDQLISSANEPSSLTFLSHLDSVQPLDLQQNTEDRLGQIIRSSGLEAFDYSESERETTQWIDTKIPEPSKSKMQALWNESQNLFSSITSQQSAEDSIEAEYQFQRNLVTCLPILDTLPIPGCIWRRSGEIYKTNEQFNHLVGIHSFPCFSLLSSFDSHHHKKSGEFIKIHQLWDIDSGLNFLEKFNRIALDEGQKAVLTSCVLYRFKEKEEVQPTTRKAEEPRPPSRSSSSSRSTTHEAKQNPDQPSVSNQRQPEGENGHQKTDELDGPKLISATSSDPLIDPTPPLLLLKETTEQPYEFVDAGKTFVVADRVNCTFSFTVRRDSFGLPCLIIGNFLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3KI63</accession>
    <name>E3KI63_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome P450-dit2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_09701</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EFP83988.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328407.2">
        <property type="nucleotide sequence ID" value="XM_003328359.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KI63"/>
    <dbReference type="STRING" id="418459.E3KI63"/>
    <dbReference type="GeneID" id="10532681"/>
    <dbReference type="KEGG" id="pgr:PGTG_09701"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09701"/>
    <dbReference type="eggNOG" id="KOG0157">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_27_2_1"/>
    <dbReference type="InParanoid" id="E3KI63"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002401">
        <property type="entry name" value="Cyt_P450_E_grp-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24296">
        <property type="entry name" value="CYTOCHROME P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00463">
        <property type="entry name" value="EP450I"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0349">Heme</keyword>
    <keyword evidence="4 5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="5">
        <location>
            <position position="463"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR602401-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP83988.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="533" mass="60298" checksum="D2FB2893219794F3" modified="2012-04-18" version="2">MSSYVSGKLVVCGLLYLIYLLIKYRDRAIGTSKRKDPSFTELPGWPLLGQLPNMLKSRSRILEASTMTSLKVGPGFSFTIPGNRVIDISKPEWLEYVQKTNVKNYAKGPLRKDIMLDVLGRGIFVVDDALWKRARHATVSIFTPSTFKTIISPSVNQSIDGLTQVLCSAHKENRTVDICDLFFRFTLDSFIRMTFGKDLGILGAEYGSHSESSAPEFARAFDVAQDQLDFRLAMVVGWELIERLNVRSMGQRMKESCRILDEFVYSLIDERLANLSHKSDFQDQDPSHPDLLSLFITTRDERGGGLTRTELRDTAMNLIIAGRDTTAQALSWAFFHLLMNKELVSKIRKETIEVLGGDQESVTYDNYKRFVWPQAVLHETLRLHPSVPKNAIVALSDDKIPGGPTIEAGNLIRWSDWQMGRDPSVWGADCGEFKPERWIDEAGSIRQFGQFKFHAFNGGPRVCLGINLAIFEAVKVMVEVLRDFELEFAEGWLENVPKSVTIDGLTSQYQTPMYKSSITLPMENPMMIAVKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KI95</accession>
    <name>E3KI95_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09733</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EFP84020.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328439.2">
        <property type="nucleotide sequence ID" value="XM_003328391.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KI95"/>
    <dbReference type="GeneID" id="10532715"/>
    <dbReference type="KEGG" id="pgr:PGTG_09733"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09733"/>
    <dbReference type="HOGENOM" id="CLU_193192_0_0_1"/>
    <dbReference type="InParanoid" id="E3KI95"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="10"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84020.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="84" mass="9401" checksum="A7FA8FAEEDE8CDF8" modified="2012-04-18" version="2">MDASQDNAMSQSESLSNSQQQSSSVQPGQPTTSTDKSKDPPAPKRGRRTKKEMAEWRDQQKRIRLPKEQDREMAKADQGARKAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJL8</accession>
    <name>E3KJL8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ornithine decarboxylase antizyme</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_10213</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Ornithine decarboxylase (ODC) antizyme protein that negatively regulates ODC activity and intracellular polyamine biosynthesis in response to increased intracellular polyamine levels. Binds to ODC monomers, inhibiting the assembly of the functional ODC homodimer, and targets the monomers for ubiquitin-independent proteolytic destruction by the 26S proteasome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Interacts with ODC and thereby sterically blocks ODC homodimerization.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ODC antizyme family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178290">
        <property type="protein sequence ID" value="EFP84493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328912.1">
        <property type="nucleotide sequence ID" value="XM_003328864.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJL8"/>
    <dbReference type="GeneID" id="10546003"/>
    <dbReference type="KEGG" id="pgr:PGTG_10213"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10213"/>
    <dbReference type="HOGENOM" id="CLU_071661_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJL8"/>
    <dbReference type="OMA" id="VKNTQHQ"/>
    <dbReference type="OrthoDB" id="5959761at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008073">
        <property type="term" value="F:ornithine decarboxylase inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0075523">
        <property type="term" value="P:viral translational frameshifting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.60:FF:000008">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002993">
        <property type="entry name" value="ODC_AZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038581">
        <property type="entry name" value="ODC_AZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10279">
        <property type="entry name" value="ORNITHINE DECARBOXYLASE ANTIZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10279:SF10">
        <property type="entry name" value="ORNITHINE DECARBOXYLASE ANTIZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02100">
        <property type="entry name" value="ODC_AZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0688">Ribosomal frameshifting</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022758"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP84493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="254" mass="27752" checksum="9039C30DF87971E8" modified="2011-01-11" version="1">MEEMPPDDYSEGGRDRHVRDVTAPNFPNPSSGPVFVKFPSSTTVSPITPSSSPTGIDCHSLAPTPPSTQNRLNNNNNNNNNKMMMASLDFKTRRPSVHNARPNPTPLLTPCSSSDSLSLSSNGFNNTRHSTPAILTKIFPNGIPDVEAVHDISTQLPNALKGAIVDRDSGIRSLYILGLTSSVTIDRQVRDIVVRVLDLADEEIEADQVFFALEKDHPMFRELLQGLLYVGGNVVKNAQHQPVNKHLVLVGIEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3KK89</accession>
    <name>E3KK89_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Coenzyme Q-binding protein COQ10 START domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_10873</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Required for the function of coenzyme Q in the respiratory chain. May serve as a chaperone or may be involved in the transport of Q6 from its site of synthesis to the catalytic sites of the respiratory complexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Interacts with coenzyme Q.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the COQ10 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329133.1">
        <property type="nucleotide sequence ID" value="XM_003329085.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KK89"/>
    <dbReference type="FunCoup" id="E3KK89">
        <property type="interactions" value="175"/>
    </dbReference>
    <dbReference type="GeneID" id="10526681"/>
    <dbReference type="KEGG" id="pgr:PGTG_10873"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10873"/>
    <dbReference type="HOGENOM" id="CLU_079653_1_1_1"/>
    <dbReference type="InParanoid" id="E3KK89"/>
    <dbReference type="OMA" id="IDGPFKY"/>
    <dbReference type="OrthoDB" id="292693at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048039">
        <property type="term" value="F:ubiquinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044996">
        <property type="entry name" value="COQ10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005031">
        <property type="entry name" value="COQ10_START"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023393">
        <property type="entry name" value="START-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12901:SF10">
        <property type="entry name" value="COENZYME Q-BINDING PROTEIN COQ10, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12901">
        <property type="entry name" value="SPERM PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03364">
        <property type="entry name" value="Polyketide_cyc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55961">
        <property type="entry name" value="Bet v1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Coenzyme Q-binding protein COQ10 START" evidence="4">
        <location>
            <begin position="38"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF03364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP84714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="207" mass="23695" checksum="BAEA12B0040F760C" modified="2011-01-11" version="1">MFHSRALKLSSQIANNSPRKFSTSTIRTGSHQLLKNLPYERSKIYSVIVDVQSYPEFIPYCLSTRIIEPVTQLDQKKENHKEQDSQSTRSNLINHQFEVFTRIGYKGFETDYHSQVICEPFESVRVIAKPSSTFTNLTTTWTLSDPPCSSTSSTTTSNSKPVTQVGLDLSFKFKNPIHEYLLTDQAIWKKFSTLIIKSFEDRLASLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3KKM2</accession>
    <name>E3KKM2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_10318</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178292">
        <property type="protein sequence ID" value="EFP84847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329266.1">
        <property type="nucleotide sequence ID" value="XM_003329218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KKM2"/>
    <dbReference type="GeneID" id="10526821"/>
    <dbReference type="KEGG" id="pgr:PGTG_10318"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10318"/>
    <dbReference type="HOGENOM" id="CLU_3107462_0_0_1"/>
    <dbReference type="InParanoid" id="E3KKM2"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP84847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="51" mass="5920" checksum="72B0BFA8E4EEE3C1" modified="2011-01-11" version="1">MTHKHLSLKVYVDVQSDAQSAQKCLKYSRLQTSPKIRWARDLSHARNHQGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KL21</accession>
    <name>E3KL21_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11165</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP84996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329415.1">
        <property type="nucleotide sequence ID" value="XM_003329367.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KL21"/>
    <dbReference type="GeneID" id="10526973"/>
    <dbReference type="KEGG" id="pgr:PGTG_11165"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11165"/>
    <dbReference type="HOGENOM" id="CLU_031648_0_0_1"/>
    <dbReference type="InParanoid" id="E3KL21"/>
    <dbReference type="OrthoDB" id="2500347at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="543" mass="62083" checksum="47B40B6D68E92BFA" modified="2011-01-11" version="1">MNPEQPTGAGRHKDDKEGLFRPHPSSSWPHVWKLDWTNPSFITQVSSHDGSGRKTLVATDSNSGISQLAPDLLHSQDDGSVSVSSERVSDMEEWTAREPKGKKARHNLIRLDTSSLKSFYYSSKNQILIAVIKDAQSSFEKRVNSEDYHVNTFRNEDIHPKLRFAMVNGSKGRIFRVLGKSRDVVQSAYTLMAHYKRLIAYIYELHQTLLSRLNLSPPAQRLQHARLFKWLETEIFKPADHRLPVIGIVPKKLSSWADIPEERLGETWIKLITYFCEKNSEQGQQELTTKTAGYIVETYQDQHEDEYIAASRPSTVNKSSLDRLLQDERFQKARSVLTSLMVDNSEIEFVIGQPLRQSARSDQLISSYCSQLQGIWGKESLNRNGSRSVHPTVRLAAYFLENMPDYAHLRIVTAQSVLLPMEMNVSMFKSFLKLVHHLHFKFLKSIGNLEPEKNIMLKDIYEWIIGMVLKPLKPSSSLPIIGPIVVNGKIAPWEEFSNEGLELFEPVQIKLIEYFSLANMPDEDIKNTAAFVLAKFYQEHGQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLQ9</accession>
    <name>E3KLQ9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_11427</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178294">
        <property type="protein sequence ID" value="EFP85258.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329677.2">
        <property type="nucleotide sequence ID" value="XM_003329629.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLQ9"/>
    <dbReference type="GeneID" id="10527247"/>
    <dbReference type="KEGG" id="pgr:PGTG_11427"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11427"/>
    <dbReference type="HOGENOM" id="CLU_029800_0_0_1"/>
    <dbReference type="InParanoid" id="E3KLQ9"/>
    <dbReference type="OrthoDB" id="2508157at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP85258.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="393" mass="44994" checksum="D4EFBA9D0DF537EB" modified="2012-04-18" version="2">MVTVLDLPEKVLELVFDNLASDIGAFRSQAACLRLVCSKWEPFLYNRYLYRVMTFRSFDRAISFIRQIDQNSQSRPVPICRYLYIYRLDTIDDSTNFMPDRKTKVENLDALVELFSDSIEMLSIEVDKFLTLPRATIERIGRIKNLRHLTIGIYSKAAPSKILHSRQNLRVDSVPLGLPTDTECLHELLAAAQGLVTLSLEDFRPVCSPETIRPTLSNHKFSSITRLDLEIPPKPYTNGDEMIGIFHALPNLKKLKIRGLENGQSVLPLFEIVRETLEEIYIYHQEVLMSILGLEFPNLRVFKIGDWTTSLSQCLRRPMFSKAPLEVLALMIPFDETFLVLPFESLPCLKKLVFGNSPMSHKNALQDSRDLLKSCRENGVKVVLIPSQKIADY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KMQ3</accession>
    <name>E3KMQ3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_11934</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178295">
        <property type="protein sequence ID" value="EFP85578.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329997.2">
        <property type="nucleotide sequence ID" value="XM_003329949.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KMQ3"/>
    <dbReference type="GeneID" id="10544138"/>
    <dbReference type="KEGG" id="pgr:PGTG_11934"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11934"/>
    <dbReference type="HOGENOM" id="CLU_2321511_0_0_1"/>
    <dbReference type="InParanoid" id="E3KMQ3"/>
    <dbReference type="OrthoDB" id="10272637at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP85578.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="99" mass="11246" checksum="F670AECC475559DF" modified="2012-04-18" version="2">MLAIKQLLDLLKTAKNHKAFARTSLVATMLQFRKLCHSISRTVEEQCTGVRLSATTNGSGMAKISGLSWDSFEALMERWQKLGWYAVKQTHVLLKEKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNW1</accession>
    <name>E3KNW1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar-sorting protein SNF7</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Vacuolar protein-sorting-associated protein 32</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_11742</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SNF7 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178297">
        <property type="protein sequence ID" value="EFP85986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330405.1">
        <property type="nucleotide sequence ID" value="XM_003330357.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNW1"/>
    <dbReference type="FunCoup" id="E3KNW1">
        <property type="interactions" value="290"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KNW1"/>
    <dbReference type="GeneID" id="10539985"/>
    <dbReference type="KEGG" id="pgr:PGTG_11742"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11742"/>
    <dbReference type="eggNOG" id="KOG1656">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_071097_1_1_1"/>
    <dbReference type="InParanoid" id="E3KNW1"/>
    <dbReference type="OMA" id="MKQIHGG"/>
    <dbReference type="OrthoDB" id="5592979at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000815">
        <property type="term" value="C:ESCRT III complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005771">
        <property type="term" value="C:multivesicular body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032511">
        <property type="term" value="P:late endosome to vacuole transport via multivesicular body sorting pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006900">
        <property type="term" value="P:vesicle budding from membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.1710">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1060">
        <property type="entry name" value="ESAT-6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005024">
        <property type="entry name" value="Snf7_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761">
        <property type="entry name" value="CHARGED MULTIVESICULAR BODY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22761:SF10">
        <property type="entry name" value="GH13992P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03357">
        <property type="entry name" value="Snf7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0967">Endosome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="180"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="19"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00040017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00042586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP85986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="225" mass="25228" checksum="2E7B35B0ABD7DDDD" modified="2011-01-11" version="1">MSSWASWLLGRGASSGNQRDKARETIVELRQHLIMLEKKEDHLNKKIEDELRKAKANASTNKRAATAALRQKKLYEQELDRLAGRRMTLETQVNAIESANMNLETMQAMRRGADVLKGIHGNLNIDKVDATMDSIREQMDLTNEISDAISNPVGMGQELDEDEIKAELEELEQDELKSRLVGADSVPLHSPGQTERPVAARTVAQRQQEEDEDAELKELQAALAM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQU7</accession>
    <name>E3KQU7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7143 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_13054</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178301">
        <property type="protein sequence ID" value="EFP86672.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331091.2">
        <property type="nucleotide sequence ID" value="XM_003331043.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQU7"/>
    <dbReference type="GeneID" id="10540934"/>
    <dbReference type="KEGG" id="pgr:PGTG_13054"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13054"/>
    <dbReference type="HOGENOM" id="CLU_831910_0_0_1"/>
    <dbReference type="InParanoid" id="E3KQU7"/>
    <dbReference type="OrthoDB" id="2497581at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055567">
        <property type="entry name" value="DUF7143"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37592">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37592:SF1">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23631">
        <property type="entry name" value="DUF7143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="228"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7143" evidence="2">
        <location>
            <begin position="12"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP86672.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="272" mass="30582" checksum="5F913AD01D2B253C" modified="2012-04-18" version="2">MKTSSLSIDRFSEVSCLDHAIQPVPGIPDLTLNGITYSSIDFQVSLDHKSSVGFAIDKFIFELEHKAAFERVSNTEVGSLVEHREDDTKEPKMEAEERKQRARSEILLLTTYWKLYGAMDIALRVLNNQSAAKILSLLKGPGFVLNFQRARLEGKVKEIGFYLIQILKNCFNCTGIERKRLILLARASGVRNQSISEAEALANSSNKTQSPTALTSGSSQSQRLLKGLWHTHAYFIFTFSLLLILYFHSINDAILHVCSMNPYQLLAEQRGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3KRH4</accession>
    <name>E3KRH4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pterin-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12640</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178303">
        <property type="protein sequence ID" value="EFP86899.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331318.2">
        <property type="nucleotide sequence ID" value="XM_003331270.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KRH4"/>
    <dbReference type="FunCoup" id="E3KRH4">
        <property type="interactions" value="176"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KRH4"/>
    <dbReference type="GeneID" id="10541651"/>
    <dbReference type="KEGG" id="pgr:PGTG_12640"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12640"/>
    <dbReference type="HOGENOM" id="CLU_739955_0_0_1"/>
    <dbReference type="InParanoid" id="E3KRH4"/>
    <dbReference type="OrthoDB" id="615426at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005740">
        <property type="term" value="C:mitochondrial envelope"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004156">
        <property type="term" value="F:dihydropteroate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046654">
        <property type="term" value="P:tetrahydrofolate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.560">
        <property type="entry name" value="7,8-Dihydro-6-hydroxymethylpterin-pyrophosphokinase HPPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="entry name" value="Dihydropteroate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045031">
        <property type="entry name" value="DHP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035907">
        <property type="entry name" value="Hppk_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941">
        <property type="entry name" value="FOLATE SYNTHESIS PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20941:SF1">
        <property type="entry name" value="FOLIC ACID SYNTHESIS PROTEIN FOL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="Dihydropteroate synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pterin-binding" evidence="1">
        <location>
            <begin position="80"/>
            <end position="363"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86899.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="374" mass="40575" checksum="EF74E92EE627C9BA" modified="2012-04-18" version="2">MDSPSPRLKWLKPRWIIHPGLGRIATKKYSSGKHSNGSKISSNALQSLSHHDPSTRVFDSSFDNLTIPHIGIIERQFVLDPLIESVDPSREFVATQTIHPETGTPIEALVADLKQKTSSSAETTGRGSVRKTHLIRGPGCQTLDLISRTHLMSIVNCTPDSFLDGGASLAVEDAVRNSLDGRDGDRTDRTRDQAATIAGATIVNDVSGGEADEAMLATVARFDVPYMLMRMRGKPRTMTQLTSYQDRDVVAGVRRELAAKVSNAGSWNLIIDPGFGFDKDLAGNYQDCLRGFPILVGLSRKQFLAKLLTLPPPGTTTTTTPPPPLLRPDQQLIPAIVASTIAICNGAHLTRAHDTKIIKKVINIVGGIRAFNSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>E3KSB3</accession>
    <name>E3KSB3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 6">Phosphoinositide phospholipase C</fullName>
            <ecNumber evidence="1 6">3.1.4.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_13407</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-4,5-bisphosphate) + H2O = 1D-myo-inositol 1,4,5-trisphosphate + a 1,2-diacyl-sn-glycerol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:33179"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17815"/>
            <dbReference type="ChEBI" id="CHEBI:58456"/>
            <dbReference type="ChEBI" id="CHEBI:203600"/>
            <dbReference type="EC" id="3.1.4.11"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="3.1.4.11" evidence="1 6"/>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EFP87188.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331607.2">
        <property type="nucleotide sequence ID" value="XM_003331559.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KSB3"/>
    <dbReference type="FunCoup" id="E3KSB3">
        <property type="interactions" value="123"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KSB3"/>
    <dbReference type="GeneID" id="10537418"/>
    <dbReference type="KEGG" id="pgr:PGTG_13407"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13407"/>
    <dbReference type="eggNOG" id="KOG0169">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002738_1_2_1"/>
    <dbReference type="InParanoid" id="E3KSB3"/>
    <dbReference type="OrthoDB" id="269822at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004435">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate phospholipase C activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048015">
        <property type="term" value="P:phosphatidylinositol-mediated signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00275">
        <property type="entry name" value="C2_PLC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16207">
        <property type="entry name" value="EFh_ScPlc1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13360">
        <property type="entry name" value="PH_PLC_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd08558">
        <property type="entry name" value="PI-PLCc_eukaryota"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000404">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.190:FF:000069">
        <property type="entry name" value="Phosphoinositide phospholipase C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000008">
        <property type="entry name" value="C2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001192">
        <property type="entry name" value="PI-PLC_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037755">
        <property type="entry name" value="Plc1_PH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015359">
        <property type="entry name" value="PLC_EF-hand-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000909">
        <property type="entry name" value="PLipase_C_PInositol-sp_X_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001711">
        <property type="entry name" value="PLipase_C_Pinositol-sp_Y"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336:SF36">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 4,5-BISPHOSPHATE PHOSPHODIESTERASE BETA-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10336">
        <property type="entry name" value="PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00168">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09279">
        <property type="entry name" value="EF-hand_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00388">
        <property type="entry name" value="PI-PLC-X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00387">
        <property type="entry name" value="PI-PLC-Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00390">
        <property type="entry name" value="PHPHLIPASEC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00239">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00148">
        <property type="entry name" value="PLCXc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00149">
        <property type="entry name" value="PLCYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49562">
        <property type="entry name" value="C2 domain (Calcium/lipid-binding domain, CaLB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50004">
        <property type="entry name" value="C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50008">
        <property type="entry name" value="PIPLC_Y_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="4 6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0807">Transducer</keyword>
    <feature type="domain" description="PI-PLC Y-box" evidence="9">
        <location>
            <begin position="560"/>
            <end position="678"/>
        </location>
    </feature>
    <feature type="domain" description="C2" evidence="8">
        <location>
            <begin position="673"/>
            <end position="820"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="461"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="506"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="465"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="516"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP87188.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="841" mass="93937" checksum="0526A2A7B175C404" modified="2012-04-18" version="2">MSRSMIDHVQVPAQLKAGTLMLKVSAKKTIKTNVSLEYERGQIVWETKKGGSLNIESVREIRFGQDASYYRTQFKLSPELEGRWLTIIYLSKKSTYKMLHLIACDPDTFALWKETLLRILAFRRELLGGLNHMRKRQDYWLKQNWSQGDSTEDAKLDFQEIVNLCRKLNISVPQSDIQAHFDIADSQKRGYLDFPAFQHFVKLMKRRPDLESLFQQFTSGRAVMSVDEFTRFMQNVQHSELTPSTIHAIYTKFLDDDNDDDREREARELNPTKQMTIEGFASFLQSADNAALHEKHTAVHQDMSLPLHDYFISTSHNTYLVGNQLKGESTVEGYIRALQHGCRSVELDCWDGDAGEPVIYHGRTLTRKLQLSEALKAISKHAFVASPYPLILSLEVHCQPAQQERLAELLKTCLGEALVDRPLENLSSESLPSPKDLMYRILVKAKKPIVGGDGPRSTEIYSNFDSSATSTTSGSSTSSDSDLKRRISNASTVLGERFKRIASAMSIPSPPVERSLPTTTDQSPTLTGQSGSAALIPGPSVGSLAPPPSKIPTAPLTNSLSSLIVYTVGVKARGFNKLTTYEKEHILSLSERTVNKFLKAGPLRMLDLIQHNRSHLTRAYPAATRVFSSNFLPHRFWAVGLQLVACNWQTHDLAMELNSAFFDQNGRSGYLLKPLALRVKGKEKDAATKMHKYQLDITIISIQQLPKLTIEDDNAGIDPCVEVQLISPVPNSSCTSPPQSTKRRTKVVKGNGFNPVYHEEFKIQFQTPGEMVDLCFLRLLVIDAGQGSGNGAKEEDGLVGKVCVCLGGLQPGYRHLPLHDDSGQQLLFASIFINSSLSKLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KT31</accession>
    <name>E3KT31_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13645</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178306">
        <property type="protein sequence ID" value="EFP87417.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331836.2">
        <property type="nucleotide sequence ID" value="XM_003331788.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KT31"/>
    <dbReference type="GeneID" id="10538146"/>
    <dbReference type="KEGG" id="pgr:PGTG_13645"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13645"/>
    <dbReference type="HOGENOM" id="CLU_1094736_0_0_1"/>
    <dbReference type="InParanoid" id="E3KT31"/>
    <dbReference type="OrthoDB" id="2510222at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87417.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="254" mass="27834" checksum="A157AD34F7C19974" modified="2012-04-18" version="2">MTSSRNCTPTGNEQQFPEAHARPTFSIAEYSTIADVTNHNGLESEHEHESNGEAALTRGSDDSWESKEDRTKYTTESNTDVTLGSKDTPDSKEYKAEDTSDSEEDFDDTSAADVTLGSDNTESEESKAEDTLDNKEGFDNTSNGDVEDTMESKDDETIASRGDLTLGSEDAVENKEEENVIGSGSNAKIVGREDTVDEDEDKLMSNTNTVEGTIDEDKEDELGSNLEVEQDAQEDELDSNHKMEKEKLASNLNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3KU27</accession>
    <name>E3KU27_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Beta-glucuronidase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14517</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178309">
        <property type="protein sequence ID" value="EFP87802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332221.1">
        <property type="nucleotide sequence ID" value="XM_003332173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KU27"/>
    <dbReference type="STRING" id="418459.E3KU27"/>
    <dbReference type="GeneID" id="10534700"/>
    <dbReference type="KEGG" id="pgr:PGTG_14517"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14517"/>
    <dbReference type="eggNOG" id="ENOG502R0IR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022148_4_0_1"/>
    <dbReference type="InParanoid" id="E3KU27"/>
    <dbReference type="OMA" id="TGTWPPI"/>
    <dbReference type="OrthoDB" id="2831684at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052974">
        <property type="entry name" value="GH79_Enzymes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36183">
        <property type="entry name" value="BETA-GLUCURONIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36183:SF2">
        <property type="entry name" value="BETA-GLUCURONIDASE C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174128" description="Beta-glucuronidase C-terminal domain-containing protein" evidence="1">
        <location>
            <begin position="28"/>
            <end position="526"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="526" mass="57422" checksum="65569D7319C580A9" modified="2011-01-11" version="1">MVRSAGWCLLLSFVGLLVVPFMDRVGGAEYDDDDDLGVAEPPKLIVVDVVEHLNASHVPVPIGDTNPLGISQFFAFPAYATRLSQTAQCLKNLEQAYGMPSPIRIGGTTQDRARYDREESQPVRYQLPSGGGAAQVPARLSFGPEFIRLANQLSPGPLTFGLNRQSGNLLNAGIAARDLVDHLSNLYAIELGNEPEFWAPGSPERKGLKNWTPDADARSQKAWQREISTRVAKKALVQAGVFLSPPRWSVSELAPQEGPDLQYVKSFGGHAYPQSACGNSKTSLAGLMNHTQIVSFVGRFEGEVRAAKKLGKPYHFSETNSATCGGHGISGTFGAALWLIDYVFQALLLGVNRLYFHQGTINHSPYSFWNATQVSPTYYGAYFVSLALRGATELSRLPSSRPEVAVYGLWKCGRLVRLVIYHSEFRGPDSPARSPEPVEIHGLPPDVLRVRLLRLAAKHAFVSATGMLSPDHVSFGNTFFDNRDCTFRSPPLYQTLPVPSGPLQLHIAPSQAIIIELVSEIPAPFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVJ3</accession>
    <name>E3KVJ3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glyoxal oxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_14417</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178313">
        <property type="protein sequence ID" value="EFP88333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332752.1">
        <property type="nucleotide sequence ID" value="XM_003332704.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KVJ3"/>
    <dbReference type="STRING" id="418459.E3KVJ3"/>
    <dbReference type="GeneID" id="10535805"/>
    <dbReference type="KEGG" id="pgr:PGTG_14417"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14417"/>
    <dbReference type="eggNOG" id="ENOG502QPS4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009630_3_0_1"/>
    <dbReference type="InParanoid" id="E3KVJ3"/>
    <dbReference type="OMA" id="NKPNPGQ"/>
    <dbReference type="OrthoDB" id="2019572at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02851">
        <property type="entry name" value="E_set_GO_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.80">
        <property type="entry name" value="Galactose oxidase/kelch, beta-propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011043">
        <property type="entry name" value="Gal_Oxase/kelch_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037293">
        <property type="entry name" value="Gal_Oxidase_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009880">
        <property type="entry name" value="Glyoxal_oxidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015202">
        <property type="entry name" value="GO-like_E_set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32208:SF96">
        <property type="entry name" value="GLYOXAL OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32208">
        <property type="entry name" value="SECRETED PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07250">
        <property type="entry name" value="Glyoxal_oxid_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09118">
        <property type="entry name" value="GO-like_E_set"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50965">
        <property type="entry name" value="Galactose oxidase, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172866" description="Glyoxal oxidase" evidence="2">
        <location>
            <begin position="23"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="domain" description="Glyoxal oxidase N-terminal" evidence="3">
        <location>
            <begin position="76"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="domain" description="Galactose oxidase-like Early set" evidence="4">
        <location>
            <begin position="457"/>
            <end position="564"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF07250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF09118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP88333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="637" mass="68442" checksum="58A938E99333E72D" modified="2011-01-11" version="1">MALHSLLSTLLLSLSLSKLISTQAIRTGKTPNTFEVVGHSGASAQMMFIGTPTKTYVVDKVENNELKLNGLPVWGTSYDTESNTATPMPINSNTFCAGGNVLGNGTWLNVGGNLAVSYGGLNVANNFDPYKNKDGGKSMRLLNPCNDNSCQWAESTPMTTRRWYPTLETLEDGSIIIIGGDDWGGYVNDKGQNNPTYEFFPSKGNVTGLNLLAISLPANLYPLTWLLPSGNLFINSNWNNAILDYKTNTEFQIPNVPHAVRTYPGSAANAMLPLTPANNYTASLLFCGGTNLQPDQWKLDWNIAAYPADATCVRITPDVDTNWRDDDSMPEGRSMGNFIFLPDGRLFLLNGIAKGTAGYGNTSWALGQSFGDGPIYEPAYFDPNAPQGSRWSRPSDLKPSTVARMYHSVALLLPDGSIQSSGSNPNADYVAPGTPGYPYFTEYKVERFYPDYYNKPRPKPTGLPTTISYGGNFFDLKLPKEDISANDALEQTKVVIIRPGFSTHAINMGQRYVQLSSTYETNSDGSATLHVSQLPPNPAVLAPGPAFIYVVVKGVPSIGAMVMVGNGQLGAQPTAAQPTLPGKTPAIVQKTETAAEMGSVAGGKIAAKKGASTRSASISITNLLALVGLATLFNWLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVL7</accession>
    <name>E3KVL7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14441</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178313">
        <property type="protein sequence ID" value="EFP88357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332776.1">
        <property type="nucleotide sequence ID" value="XM_003332728.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KVL7"/>
    <dbReference type="GeneID" id="10535829"/>
    <dbReference type="KEGG" id="pgr:PGTG_14441"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14441"/>
    <dbReference type="HOGENOM" id="CLU_034873_0_0_1"/>
    <dbReference type="InParanoid" id="E3KVL7"/>
    <dbReference type="OMA" id="HIDPLQQ"/>
    <dbReference type="OrthoDB" id="2500432at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174147" description="Secreted protein" evidence="1">
        <location>
            <begin position="21"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="558" mass="59554" checksum="3C7B3554236C1E4B" modified="2011-01-11" version="1">MRHFGLNFLVVSLSLSQAHGNQYQPSDASSYQTAAPATVSGVSTAFVSGQKAVTAQLKSDAHAAIQDFSDAITTAAGNSSTQSSAETAQLFNTIVKGAMSAPINQNIGSSATPSQDRLNSMAASINATLNSINDAANKILSKSNGALHYQDLKDCIGGMVQQGGLHDGESCTMNGMSITGVSNIMTNVLKNFGNNAIPPAVIHEATKALDPSFTAIIPGEKTFYDSVNNAITSVQASVSGQLIAALNDAQNCFESAMKSEGSYEQKMQMTQECNRSPTGKSVYNDLKTLYLSVTNQFVGYLQPNVIDSVHDIAEHYLDKNDTASDEAYFRQAVRNTTSSLVASNAGNQVTYAYSLANCLDSVIGTTDPHAASNIANTKDCLSKPDGPPASVKQIVYGYIQQIYGVLPAQIAAKIEASGVDKLDPADPQYQSKVAALHDTFEKTNVGPEYVTCYEAFVDCLFNERNGALEKPGNTQSVCLLGDACQKAPDGKANLAVPFGRRSLTSNAAHLLRRLAEMPTKTSPHPVQKARIVRKYTITLPHRSYLLNSYHQQFNSSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3KWQ3</accession>
    <name>E3KWQ3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">40S ribosomal protein S30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_14686</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS30 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178316">
        <property type="protein sequence ID" value="EFP88720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333139.1">
        <property type="nucleotide sequence ID" value="XM_003333091.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KWQ3"/>
    <dbReference type="FunCoup" id="E3KWQ3">
        <property type="interactions" value="180"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KWQ3"/>
    <dbReference type="GeneID" id="10541510"/>
    <dbReference type="KEGG" id="pgr:PGTG_14686"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14686"/>
    <dbReference type="HOGENOM" id="CLU_010412_5_1_1"/>
    <dbReference type="InParanoid" id="E3KWQ3"/>
    <dbReference type="OMA" id="YNTQNVP"/>
    <dbReference type="OrthoDB" id="199599at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006846">
        <property type="entry name" value="Ribosomal_eS30"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12650">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S30/UBIQUITIN-LIKE PROTEIN FUBI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12650:SF15">
        <property type="entry name" value="RIBOSOMAL PROTEIN S30, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04758">
        <property type="entry name" value="Ribosomal_S30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="26"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP88720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="66" mass="7279" checksum="C6B2E39902A26496" modified="2011-01-11" version="1">MGKVHGSLARAGKVRSQCPKVAKQERTKKKLQGRAKKRCLYNNRFAITTPQGGRRKMNPAPAGKMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3KWU6</accession>
    <name>E3KWU6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14729</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178316">
        <property type="protein sequence ID" value="EFP88763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333182.1">
        <property type="nucleotide sequence ID" value="XM_003333134.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KWU6"/>
    <dbReference type="GeneID" id="10541714"/>
    <dbReference type="KEGG" id="pgr:PGTG_14729"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14729"/>
    <dbReference type="HOGENOM" id="CLU_894699_0_0_1"/>
    <dbReference type="InParanoid" id="E3KWU6"/>
    <dbReference type="OMA" id="FEFWNIS"/>
    <dbReference type="OrthoDB" id="2506772at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="311" mass="35910" checksum="A6A6C363F3FDD933" modified="2011-01-11" version="1">MTPSTSQPIMLDLASDDSSSDEKVNDAPMAEDAQARKGKTPAKSKKPKPPETQLPHRLSSLTRSIVEFAKFMMGTTGRDNYHLPDPPTSEELKQYEAWGIDYVQAVDAFHQEQASRNDIEDEAEREHLKKKELKELELSLQSTRYEPAPIPPNYEISKHFKDQCDQSFRLKGFPRITFQWNKPSLEGSSWNFATADILANQWHSWYIKDRRYSPSTKLTVDAKGVIGRWLKTAAMNQAQKPKEESSENSTRTTQEMNTLRRHREQRRIQIARHRHASAMRSVPKQHKEYLSELLSANDMSSDYEDDEDPEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KX03</accession>
    <name>E3KX03_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178317">
        <property type="protein sequence ID" value="EFP88896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333315.1">
        <property type="nucleotide sequence ID" value="XM_003333267.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KX03"/>
    <dbReference type="GeneID" id="10542174"/>
    <dbReference type="KEGG" id="pgr:PGTG_14235"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14235"/>
    <dbReference type="HOGENOM" id="CLU_100694_0_0_1"/>
    <dbReference type="InParanoid" id="E3KX03"/>
    <dbReference type="OrthoDB" id="2508085at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="215" mass="24111" checksum="800517540D4CF29C" modified="2011-01-11" version="1">MHPNTDEQDVSITSADGNKTTIDEPITDQGTSSSKKIFSVPFVYLDRNLATGTPGEAHGFTHEHGNQSTNHNEQVASAGGTAVEADELFTDEELADVFRVLFHPGGSAGIFRCSLTPGKIDQVIQELVATYDFTSPFNMHYIDTPEGKMPADYILEMMLKQWQATGYFSRWKYYYPDYLAFSSMEYDNDNFKALVGFIAGTESDNWTWTIIHQAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KXL5</accession>
    <name>E3KXL5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14918</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178319">
        <property type="protein sequence ID" value="EFP89077.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333496.2">
        <property type="nucleotide sequence ID" value="XM_003333448.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KXL5"/>
    <dbReference type="GeneID" id="10546546"/>
    <dbReference type="KEGG" id="pgr:PGTG_14918"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14918"/>
    <dbReference type="HOGENOM" id="CLU_1971612_0_0_1"/>
    <dbReference type="InParanoid" id="E3KXL5"/>
    <dbReference type="OrthoDB" id="2500779at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89077.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="127" mass="14143" checksum="3E4B21A3443C7138" modified="2012-04-18" version="2">MKTFKTRLIEVGWSLPADSIGHLVMDKFPASLDNIANVITHTGKEITLDTVVDHLRLHAHNLESRAIGNGTKSDPITLFTDSSRRCKRNAHNTLSSHSESNCWMLHPELRPAKKDSANKTEATNTKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KXZ9</accession>
    <name>E3KXZ9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_15041</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178320">
        <property type="protein sequence ID" value="EFP89200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333619.1">
        <property type="nucleotide sequence ID" value="XM_003333571.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KXZ9"/>
    <dbReference type="GeneID" id="10546837"/>
    <dbReference type="KEGG" id="pgr:PGTG_15041"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15041"/>
    <dbReference type="HOGENOM" id="CLU_1205272_0_0_1"/>
    <dbReference type="InParanoid" id="E3KXZ9"/>
    <dbReference type="OMA" id="HPSQFRS"/>
    <dbReference type="OrthoDB" id="2505803at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP89200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="230" mass="26194" checksum="2796300B7ACDDAFB" modified="2011-01-11" version="1">MTSIVRTACRSVAQISRNAIANQLLRTQSPTLTAQIRSYATAKKAVRVNVAPTVIPPKDGNKNSPDDKVLMLDVKKKVLDLLEKEGPMSRRKMYDDHLSSIYPTAPFVPNPPPRPLWLRTQYSPEEERVYLTMSKFKRQLIRSLTADGTVSVITVRKVRHLLAEVDESDTSEELAHRKKKMESVVASETEKTFVWILDSWITTLKQKSLERCQREAKLLGHTPPTRFKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3KY59</accession>
    <name>E3KY59_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Queuosine 5'-phosphate N-glycosylase/hydrolase</fullName>
            <ecNumber evidence="1">3.2.2.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Queuosine-nucleotide N-glycosylase/hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15101</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the hydrolysis of queuosine 5'-phosphate, releasing the nucleobase queuine (q). Is required for salvage of queuine from exogenous queuosine (Q) that is imported and then converted to queuosine 5'-phosphate intracellularly.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>queuosine 5'-phosphate + H2O = queuine + D-ribose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:75387"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17433"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:194371"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:75388"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the QNG1 protein family.</text>
    </comment>
    <dbReference type="EC" id="3.2.2.-" evidence="1"/>
    <dbReference type="EMBL" id="DS178320">
        <property type="protein sequence ID" value="EFP89260.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333679.2">
        <property type="nucleotide sequence ID" value="XM_003333631.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KY59"/>
    <dbReference type="GeneID" id="10546899"/>
    <dbReference type="KEGG" id="pgr:PGTG_15101"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15101"/>
    <dbReference type="eggNOG" id="ENOG502S4MU">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053189_0_0_1"/>
    <dbReference type="InParanoid" id="E3KY59"/>
    <dbReference type="OrthoDB" id="416777at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006400">
        <property type="term" value="P:tRNA modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019438">
        <property type="entry name" value="Q_salvage"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21314">
        <property type="entry name" value="QUEUOSINE 5'-PHOSPHATE N-GLYCOSYLASE_HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21314:SF1">
        <property type="entry name" value="QUEUOSINE SALVAGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10343">
        <property type="entry name" value="Q_salvage"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU365002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89260.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="366" mass="40943" checksum="A071DB9994933B6E" modified="2012-04-18" version="2">MTSSQLETAPLPADDITIADAIKASCEKCLDNYSISINKTACEQFFLNLDRKDYEKRVDAEPANLKFPLQFSSQLDELNVVAMVCLLEGFHSYEDLLKEKTGRGVWETMRYLVLAAYLSPESQSTSILTTRGMIEATPEYISTTLSLPLHIERPIAENSPISLAEPDRPVTRFVEDLLSLLHSTGKILQQSGLPNLASFLATRSNLNDDEMIRQLAAAFSPFRDMYTIDGKNVYLMQKVLFLIHKIQNTPISFPKLKSPRPILADNILPVFLVQAGLLDISKSTDSSLRNLNLSDSHLKISSRSATILRASSVACCQFLIDLVQGLSQTNKSLEYVEQDGLVRYLSNLSRERSSSCAFREEETVVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3KYC5</accession>
    <name>E3KYC5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_15495</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178321">
        <property type="protein sequence ID" value="EFP89316.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333735.2">
        <property type="nucleotide sequence ID" value="XM_003333687.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KYC5"/>
    <dbReference type="STRING" id="418459.E3KYC5"/>
    <dbReference type="GeneID" id="10547037"/>
    <dbReference type="KEGG" id="pgr:PGTG_15495"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15495"/>
    <dbReference type="HOGENOM" id="CLU_032336_0_0_1"/>
    <dbReference type="InParanoid" id="E3KYC5"/>
    <dbReference type="OrthoDB" id="2504961at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="62"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="318"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="379"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="550"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="241"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="63"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="555"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="568"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="586"/>
            <end position="596"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP89316.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="596" mass="66337" checksum="9CC4B75077CFB467" modified="2012-04-18" version="2">MPSADDDLPDPALDHSPIGGGGKTFLKPFVKGVASAGRSVGAGIYHAGKVVDAITDLTERISLPRNNNGSPNLSSPSHAEFGPPLDTSEVARVPEDRREKPKRFKAFKKAGSSGPGNSSDAGSEGYVSQAGISRLNSLELSRDEFPHLDKHMSRMRYSRAHTDHIGLQKCDDEELETSMVINSDDELNGPTDRATAVYNYEAVFPKTRAQSVCKMTFAKRRHTIQIPQDYNHVPVRHSVRKQNLEVQLVWKYRQLKKMEQEQRSVLEAFEILERQYDSTAKEAEIKSREVKDRLEGYVEQNKLATELLSFLPSDPLLPSHSTTVPLTGPEHRPLHSSGDPFHQHSHQQRQLSVSARAFSWDKVDPQTWELFKQLTSSGEVLRQRRHEVSNKRRTMEHHANELIKQAGLHRPAPRRDHPSRRRLAQDSATEQPEQLSPVLKKEKRGSKSSTTEGEDAEEDEGRGAGSTDAKLVIAMVEAVVSTIERDPSLQVAEFIETVLLASLARLLNALVASFGSLLRNLLRSLLVFFVRQIHRIARCLDLDDHQLGLRHPHPPHPPSAHIPPHSPLPSSSSSSSSSSSSSGRDGLIRPESHHPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3L068</accession>
    <name>E3L068_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="5">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_16243</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="5"/>
    <dbReference type="EMBL" id="DS178327">
        <property type="protein sequence ID" value="EFP89955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334374.1">
        <property type="nucleotide sequence ID" value="XM_003334326.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L068"/>
    <dbReference type="STRING" id="418459.E3L068"/>
    <dbReference type="GeneID" id="10545770"/>
    <dbReference type="KEGG" id="pgr:PGTG_16243"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16243"/>
    <dbReference type="HOGENOM" id="CLU_877549_0_0_1"/>
    <dbReference type="InParanoid" id="E3L068"/>
    <dbReference type="OrthoDB" id="6086888at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="124"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="40"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="40"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP89955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="317" mass="36342" checksum="CDE93F56AF1E76C4" modified="2011-01-11" version="1">MSLHQAMPRYWKIVIADFQYRGKHPAEVHAPDSQVLQDSRELHPDSATKHNRPESQTRSQLSMNRVSKHKPPPCSPPQAAWASVGITVLKKIAEKNDQSLRNEISTLSLKHYNQEAKPRQIDAVFNLVRGRNTFLLAGTGFGKSRIPELYFRTLPIKEKAVILVLNPLDSLGDNQVLEKTRSGFTAINLTKLTICPEECEKIADGDYNFVYLSPEIFLNSKLWDRIYFSDKFQQRLGLVVVDEAHMVYEPSYGNLGGHLMTRNNMPILLMSATCRPVAIKAIRKSLKLPEETLWMLRGELTRPEIRFIRVTMDWSMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3L0Q4</accession>
    <name>E3L0Q4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15956</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178329">
        <property type="protein sequence ID" value="EFP90108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334527.1">
        <property type="nucleotide sequence ID" value="XM_003334479.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L0Q4"/>
    <dbReference type="GeneID" id="10546086"/>
    <dbReference type="KEGG" id="pgr:PGTG_15956"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15956"/>
    <dbReference type="HOGENOM" id="CLU_1321467_0_0_1"/>
    <dbReference type="InParanoid" id="E3L0Q4"/>
    <dbReference type="OrthoDB" id="2512966at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="208" mass="23121" checksum="D2242291C33BAB44" modified="2011-01-11" version="1">MRRPTTPKAFDLSPLQSFEQTRSEDQEPKDADEELQLSKRPRPTEPLEEYHWHAEQHLLSKERPSIPGLTTSSSISATVRNDEFDKADRLLHGRKEALRADPLNQKETPALHSAFGNVASHSKHLTDSQPIKISSGESKATASQNRPHLESSSAQSFRGEITALATGREGKEIQTASHTVSPVEDLQVTAWSLFPGNEQTTDETFDNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>E3L0X8</accession>
    <name>E3L0X8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">L-2-hydroxyglutarate dehydrogenase, mitochondrial</fullName>
            <ecNumber evidence="7">1.1.99.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_16481</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(S)-2-hydroxyglutarate + A = 2-oxoglutarate + AH2</text>
            <dbReference type="Rhea" id="RHEA:21252"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:16782"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="EC" id="1.1.99.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the L2HGDH family.</text>
    </comment>
    <dbReference type="EC" id="1.1.99.2" evidence="7"/>
    <dbReference type="EMBL" id="DS178330">
        <property type="protein sequence ID" value="EFP90203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334622.1">
        <property type="nucleotide sequence ID" value="XM_003334574.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L0X8"/>
    <dbReference type="STRING" id="418459.E3L0X8"/>
    <dbReference type="GeneID" id="10546345"/>
    <dbReference type="KEGG" id="pgr:PGTG_16481"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16481"/>
    <dbReference type="eggNOG" id="KOG2665">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024775_1_0_1"/>
    <dbReference type="InParanoid" id="E3L0X8"/>
    <dbReference type="OMA" id="GVHFTRM"/>
    <dbReference type="OrthoDB" id="498204at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047545">
        <property type="term" value="F:(S)-2-hydroxyglutarate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006076">
        <property type="entry name" value="FAD-dep_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43104">
        <property type="entry name" value="L-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43104:SF4">
        <property type="entry name" value="L-2-HYDROXYGLUTARATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01266">
        <property type="entry name" value="DAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD dependent oxidoreductase" evidence="9">
        <location>
            <begin position="23"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00036066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00041137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP90203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="508" mass="56609" checksum="648BF2750862E92C" modified="2011-01-11" version="1">MGSKTLSRLRQNFPYIPPEATVDHLVIGAGVVGLAIGRELVHRFRSRSTFVVERNHQPGQETSSRNSEVIHAGIYYPQDSLKTALCLRGRKLMYDYCRKHEIPHKKIGKLIISRSREETEYLQKLYQKCQSLNGSQDLRRGVMFGRMEDREEELVPLRVIDQAEAHALEPDLSPDVSFGLFSPETGVLDSHAFISSLENSIENSENGELVYGTHVVRIDRSSTDQGWVVQTQTNADQPGEGGGERNSVLAKCLVNCAGLNAHNIYNHLLYPRSRQLQLGFCKGSYYSYSSPKGVDSVKHLIYPTPIQQPTQKSFVGLGTHLTLDMNQKIKFGPDVEWLTTKMIEANKGLFGKCGPDGLAAGALEECQDFWTELLVPNDQRLETTYQSVRSYLPGVDRDHFSPDYSGIRPKLRTTDSSQNESNQEVLLNGNEGMSGSQLPNLDDFYINQSEPGFVNLLGIESPGLTSSLAIAEHVAMLIQQLPSGLSLKSRQSTAHERFSAVGHLDDWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3L1Z0</accession>
    <name>E3L1Z0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_16591</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178334">
        <property type="protein sequence ID" value="EFP90565.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334984.2">
        <property type="nucleotide sequence ID" value="XM_003334936.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L1Z0"/>
    <dbReference type="FunCoup" id="E3L1Z0">
        <property type="interactions" value="215"/>
    </dbReference>
    <dbReference type="GeneID" id="10530465"/>
    <dbReference type="KEGG" id="pgr:PGTG_16591"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16591"/>
    <dbReference type="eggNOG" id="KOG1286">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007946_12_1_1"/>
    <dbReference type="InParanoid" id="E3L1Z0"/>
    <dbReference type="OrthoDB" id="10062876at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015171">
        <property type="term" value="F:amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003333">
        <property type="term" value="P:amino acid transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1740.10:FF:000167">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF4">
        <property type="entry name" value="ARGININE PERMEASE CAN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006060">
        <property type="entry name" value="AA_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="64"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="196"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="235"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="305"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="339"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="382"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="412"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="7">
        <location>
            <begin position="33"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="7">
        <location>
            <begin position="124"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP90565.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="486" mass="53219" checksum="CF9F60B485437A2C" modified="2012-04-18" version="2">MTPSLEGNVSLESKITDDDTPSCNGRTTLQTRHVYMMAIGGTIGTGLFSGSGEALRNGGPVGLLLGHLVVGVVVYSMIVALGEMVTVFPVGGSAPKLPSPVPEDKALIHYPARFVDPALAFACGRPDHDRIGFRYWISPGPFNQFNEIPGFIGRFLAFWSVLSQAAFTHQGTEIVALAALETENPRKSIPIAIKTVLYRILIFYLGGMTVVGLLVPYTSSSLGTGARSGALSPFVIAFNSVHIPTLSMGVSIIAVMIISVFPVVNSHIYAGSKILHGLSSDDLAPLLFSRAPKWFSRYTSNGDQVAGIAITAVFGLLAYTSVAVGISQADTVYERFSKMSSISVLMTWWSILLTYIQFYHGVSRKVGIDRGMFAYQAPFQPWLSYFGFSMTTLIIIFNGFEVFLSHNWSTSKFISAYISLLIFPLGFIWSKISRKTSFVKLDQMKLEEGRSELNPLDVMEGENDVNSVGKFHKFLHWILKFISVTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3L2B7</accession>
    <name>E3L2B7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_16730</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178336">
        <property type="protein sequence ID" value="EFP90704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335123.1">
        <property type="nucleotide sequence ID" value="XM_003335075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L2B7"/>
    <dbReference type="GeneID" id="10530604"/>
    <dbReference type="KEGG" id="pgr:PGTG_16730"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16730"/>
    <dbReference type="HOGENOM" id="CLU_1807180_0_0_1"/>
    <dbReference type="InParanoid" id="E3L2B7"/>
    <dbReference type="OrthoDB" id="2536450at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34862">
        <property type="entry name" value="SPARK DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34862:SF1">
        <property type="entry name" value="SPARK DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP90704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="143" mass="15621" checksum="C87972EA7352DBF8" modified="2011-01-11" version="1">MYSILTHYDVTRDMFCTQYKQNSTYCLPSVLGDVESQSGEKITLGEVVSLITGKLTKADRAFMSVPKETYCTPCGHAIVTKSATMIDAIRKDPVGIEFNYSSSSTVHQISEICGAAFEDHQIPDSVQIAPPPKSGNPIEKKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3L2N0</accession>
    <name>E3L2N0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_16807</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178337">
        <property type="protein sequence ID" value="EFP90781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335200.1">
        <property type="nucleotide sequence ID" value="XM_003335152.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L2N0"/>
    <dbReference type="GeneID" id="10530686"/>
    <dbReference type="KEGG" id="pgr:PGTG_16807"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16807"/>
    <dbReference type="HOGENOM" id="CLU_821683_0_0_1"/>
    <dbReference type="InParanoid" id="E3L2N0"/>
    <dbReference type="OrthoDB" id="10517159at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP90781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="338" mass="36896" checksum="DA785BDD2CDFC624" modified="2011-01-11" version="1">MPVALPNLASCVCCMYICTTSPPKRKTESTELALPSGLPPSWPGSWARHFNDAFEVTTVVIPADLGTSSRTAILVIVDIASFKALTLAPRFSNMPNLHDVDTILGIQISRTQTNQQVPTRKKLDEEIVEDVGTPSTPKLQLVEASDEEHEVFLENGLNYQQCWASEPHCKHDEAGCDVCEFESVAVHGEARDEPLVGALAMLEIPQGEGAEGRPTTFDLEVWSDPNWAGDPKPISPPTTICCPAAKNAHADLAADMLHQLMPNIDSYIVSATYLPLKVISCCILGTFTCREHQGLQAPLLAAPFFAAPIDRPLCGNLSKASDTMMILKLTTLRSWWLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>E3L5B6</accession>
    <name>E3L5B6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclin-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17478</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178351">
        <property type="protein sequence ID" value="EFP91741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336160.1">
        <property type="nucleotide sequence ID" value="XM_003336112.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L5B6"/>
    <dbReference type="STRING" id="418459.E3L5B6"/>
    <dbReference type="GeneID" id="10531681"/>
    <dbReference type="KEGG" id="pgr:PGTG_17478"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17478"/>
    <dbReference type="HOGENOM" id="CLU_433551_0_0_1"/>
    <dbReference type="InParanoid" id="E3L5B6"/>
    <dbReference type="OrthoDB" id="1060854at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF94">
        <property type="entry name" value="PHO85 CYCLIN-6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08613">
        <property type="entry name" value="Cyclin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="485"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="494"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="514"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="563"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="585"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="604"/>
            <end position="625"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="625" mass="69869" checksum="EBD04A73F3A8B793" modified="2011-01-11" version="1">MNHSLTTTTTTTNQQQQTHQDGQQQQQQQQQLPLKANQHRMNCNNQLRTNQLQIQHHPTTTVDRIQLPNNNHSSNSLKESIKLNETRHTNNNKKKKIDLESFPQETLLKNLANLFDRITSRNDHLNDHTRRRPATPSSELGRKSQDLLRRRRRRRPGAETEERASTHQEDSFMTENLNYYDNHPRRDNDEDCEDDDDDDETEDEEDEDEDEGRRLNTRPTQSPTVIHFQKLNQQNTRSPYSSPLAEPSCSPNIHHSSTNKEQQLSTASLELLSKPNSILTFHAKIVPQISIEAYLLRILKYCPTSNGVFLSTLIYLDRLCTFNHCHPSSTASSSSSSQTTTTTTPLSSLSSSSSITSTTTSSTTTPTSSSSLSSSATNFNEIAKKKKKKNKWNLSPNHLTQFNFHFVIDSWNVHRFLIASITAASKLLSDVFYTNSRYAKVGGLPLEELEELEIKFLLMSDFRLMISASEFEDYTERLLGSSSSITTPTRCKLPSTSTSPPATTTTRRTIIVTEETDEESEEGMDGEGSDSDSDTDTEQDRLRSGHLNGILTPHSGLIHHESSSASSVSSCNSSERSSCSTITPHSPPPPQTPTHPPKSPPVTLPTTPRSAFASSSSSSASPSIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3L6H8</accession>
    <name>E3L6H8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ornithine aminotransferase</fullName>
            <ecNumber evidence="4">2.6.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_17883</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <reaction evidence="4">
            <text>a 2-oxocarboxylate + L-ornithine = L-glutamate 5-semialdehyde + an L-alpha-amino acid</text>
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            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="ChEBI" id="CHEBI:59869"/>
            <dbReference type="EC" id="2.6.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>pyridoxal 5'-phosphate</name>
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        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-ornithine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
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    </dbReference>
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    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="P:L-arginine catabolic process to L-glutamate"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0010121">
        <property type="term" value="P:L-arginine catabolic process to proline via ornithine"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000185">
        <property type="entry name" value="Ornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050103">
        <property type="entry name" value="Class-III_PLP-dep_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986">
        <property type="entry name" value="AMINOTRANSFERASE CLASS III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF18">
        <property type="entry name" value="ORNITHINE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU365036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP92153.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="206" mass="21806" checksum="9CC2101B7168BF8F" modified="2012-04-18" version="2">MNAGVKAVETALKLAQKWAYVEKGVLDGKAIILSVGENFHGQTLGVMGMSTDPDCQNSFGPFLTSIGSSCPINEPANSALNAHGKNTAALLLEPIQGKSEIIVPYDDYIPKVKALCESHNVLLIMHEVQTGLGQTGKLFYPVSAVLADDHILGVIKPGEHSSTFGGNPLGCAVAIAALDVLIEENLCERAEEMGKLMRSGLLFLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3L765</accession>
    <name>E3L765_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18375</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178363">
        <property type="protein sequence ID" value="EFP92388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336807.1">
        <property type="nucleotide sequence ID" value="XM_003336759.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L765"/>
    <dbReference type="GeneID" id="10538213"/>
    <dbReference type="KEGG" id="pgr:PGTG_18375"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18375"/>
    <dbReference type="HOGENOM" id="CLU_1120593_0_0_1"/>
    <dbReference type="InParanoid" id="E3L765"/>
    <dbReference type="OrthoDB" id="2508484at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="248" mass="27308" checksum="44EF8F4CFDFFE58E" modified="2011-01-11" version="1">MRRGDILASFLGLLLCPSSRNLLRFSIAVPLEDHTRALTNVHSIVKRATPSQPSAPTDSVPDVLEKPAPEEDLVPDVPTDLFPQDIASLQFAWNHLLEDDHHGVGLALPETEIDNKIGQLNADLVQALKGDDKPMSPEAITDELEHAPKETTRPKMSRQLPSAESFESGIEFNRLAQRRKMIPESSNSQADRHLSSTKISQVGPNTKILEPSTGSEKNLILDRPTPSWNPEPSESSGVTRGLKFPHFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3L799</accession>
    <name>E3L799_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BED-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18249</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178364">
        <property type="protein sequence ID" value="EFP92424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336843.1">
        <property type="nucleotide sequence ID" value="XM_003336795.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L799"/>
    <dbReference type="GeneID" id="10538249"/>
    <dbReference type="KEGG" id="pgr:PGTG_18249"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18249"/>
    <dbReference type="HOGENOM" id="CLU_050093_0_0_1"/>
    <dbReference type="InParanoid" id="E3L799"/>
    <dbReference type="OMA" id="CFENHTS"/>
    <dbReference type="OrthoDB" id="2504876at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF5">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="12"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="49"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="294" mass="33323" checksum="B8357E13806E58FD" modified="2011-01-11" version="1">MAHSTPRHSPTRSRTPSVPPSRRSTRIVTPLRTNARYVRTNSDCRKSLSQDPRSSSDSESSISGIPKPSRSNANPRQHQKKKNQSNEQTNTNRTQSAMDLTQDSDNENFKVTKKRKKCARKDSEFGLDEIELYFHEPFYEEGETKEGPPMHYKCKWCGIPYKKGDGSRGNLVKHRDGAANRSACNQRAKAIRSGAKLPLTAKEIASKKRSEESGTANFIHSGKFDPKILNQLIVLWIVQSSLPWSRIEDKLLRLAFRYARPGLKLNSRVWAASEAHSLYCNLQEKVVSMLQTPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3L7K9</accession>
    <name>E3L7K9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">G protein-coupled receptor GPR1 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_18532</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178366">
        <property type="protein sequence ID" value="EFP92534.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336953.2">
        <property type="nucleotide sequence ID" value="XM_003336905.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L7K9"/>
    <dbReference type="GeneID" id="10538692"/>
    <dbReference type="KEGG" id="pgr:PGTG_18532"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18532"/>
    <dbReference type="HOGENOM" id="CLU_1200327_0_0_1"/>
    <dbReference type="InParanoid" id="E3L7K9"/>
    <dbReference type="OrthoDB" id="100006at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004930">
        <property type="term" value="F:G protein-coupled receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007189">
        <property type="term" value="P:adenylate cyclase-activating G protein-coupled receptor signaling pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR 157-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23112:SF37">
        <property type="entry name" value="G PROTEIN-COUPLED RECEPTOR GPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="209"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP92534.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="231" mass="25782" checksum="F22D339C2783F908" modified="2012-04-18" version="1">METLKLGPETQMEPLHVFSHLCPSESDLPAQDVWIWLAATGLIALYGTLFTRIRTHYKGEGHILRRSSIVIPAPSTPSTMSSMSGSLSNLSKAAKRQKVGNKGLRVVARTMLVYPISFVAYSIPLTVISLIENYRRSTLNPVVALVLSTLFALRGAIDVLAFGMTRNVFILRTPPTPVYDEEQLTPTSPNSIHKTRQISLDLDKIQEIKESRDPNHSQRSHESVDVKFYHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8M8</accession>
    <name>E3L8M8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19032</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178376">
        <property type="protein sequence ID" value="EFP92914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337333.1">
        <property type="nucleotide sequence ID" value="XM_003337285.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L8M8"/>
    <dbReference type="GeneID" id="10542723"/>
    <dbReference type="KEGG" id="pgr:PGTG_19032"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19032"/>
    <dbReference type="HOGENOM" id="CLU_126146_0_0_1"/>
    <dbReference type="InParanoid" id="E3L8M8"/>
    <dbReference type="OrthoDB" id="10442106at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172523" evidence="1">
        <location>
            <begin position="21"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="184" mass="21716" checksum="04775F078D3C70CD" modified="2011-01-11" version="1">MIAPLNILFIFFALINVGGSKPALESLKKCKPDKAVGNGLLQSYFDLPESQNSRKFDYSKPNDNAARIDWSRSSNFVEISELRQGWLEIRAAIQDFECWCNPEHYELFYKLKDLLHFLKRMVQVRLYDIPKQEQIELHGDLLLDVRKKVSKEFPEYWDSLETKLVTFQIDDHFKDYFESEIEAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3L9F0</accession>
    <name>E3L9F0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_19135</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178384">
        <property type="protein sequence ID" value="EFP93175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337594.1">
        <property type="nucleotide sequence ID" value="XM_003337546.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L9F0"/>
    <dbReference type="GeneID" id="10543325"/>
    <dbReference type="KEGG" id="pgr:PGTG_19135"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19135"/>
    <dbReference type="HOGENOM" id="CLU_059061_0_0_1"/>
    <dbReference type="InParanoid" id="E3L9F0"/>
    <dbReference type="OrthoDB" id="2506670at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP93175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="381" mass="44569" checksum="0A9656CD086F0C8E" modified="2011-01-11" version="1">MEVEPHTVQILSTVDLDLLAKALARLILVLKQRIRKFDRTVTWDRKKVSSQFESEVHRIKTILLPSIPRKLHRLCDLLFDSVYLQKLEAPQLQDGIKVLKELENIVHKISSSIDSFVVDEYQFFSNRDAERWKQYRSERAQSKLADSFFNLRCLLLLYSDLLPQLGGMTITRHEAKQKLRELIDYIHFEEEREDDRIAWFDMSELSAVQEHCGEMAEEQGKLLQELVEFPTLHKDIHVQPLREFIPILKLSRLFFNKISKPTSDESHPITSMNTDQRLNLINLVASIPTKLTKFYDEMECNYEPTQNIDLSKVSDLGKLFQAPLNLLTNCLSDQGTNPNSPQDSISKLILWYANWQCHFFVAVRRFYIAYTDAYSNFLIVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3L9P5</accession>
    <name>E3L9P5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_19317</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178387">
        <property type="protein sequence ID" value="EFP93270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337689.1">
        <property type="nucleotide sequence ID" value="XM_003337641.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L9P5"/>
    <dbReference type="GeneID" id="10543502"/>
    <dbReference type="KEGG" id="pgr:PGTG_19317"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19317"/>
    <dbReference type="HOGENOM" id="CLU_020033_0_0_1"/>
    <dbReference type="InParanoid" id="E3L9P5"/>
    <dbReference type="OrthoDB" id="2510218at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP93270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="751" mass="84567" checksum="346B9EC6184A7419" modified="2011-01-11" version="1">MRIIASELSFIGFLCLVASYGALHIHNLQPRSMIFEGAESISGTAKAHEVAHEQRVQHMGTGVSSASTEIGGHSSSSTLTQDSKELMNSDHAKPVTTSPIKEGKWQKTVTRVKSKLIVMKKSFRRYMSILRSTKSIFKILMRRPNLKVNAQIVKFEDTRDSLAKVLVSPGLDRSPDKPLNVENLAKSLTREFPILPEISQEASTTSFYAEYLENLKRYQLIVSENGGVRSTWIAKIPSEEAAGTKEAVTEVSDLLQVHPHLVDEFQGEHKQVVSGIANFDQLKLSEEDIRQHFAIDLVMEEAQNSIKNNDFIKARAVQLKKMTPGDLEVLDMPHIFQHLPADLDFKTELFRYSMIERFESLLKDIRPTVTRNLENRAQAEISDVLKKSSMPDTGSESIFKIAAKEDDAWRDNAFAKLYEIPMSPSEVFQKLNKPPTPQEINQHVINKQLPSLQFLTPEGRENLRYTVSSKSFNKHLSTLETLVKKSTDLDKEKILPTLKQMRYEAAQSHSVNLPLVDKLLDKGIIDQAQRELLISKAQSPKEFAAALGTEEDFTKKLKGKFEGDLANHLQSMDQVTKEKTLIKTARGVAQSRLNQLDQDAKATYLTTDAFEKPNSFRFNDALKAYDEAGSKFIQQPADSKTLARAYIDASFSTRPVDPAAEKYLGTVAIKDELRMNLDQKLYEMENMFYEHMPKMGDFFENILKDNRKPGTPYLTSGDLSSKQVKVPYGNVNSGYLIGEVLGSKSLPKDVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3LBQ6</accession>
    <name>E3LBQ6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">alpha-galactosidase</fullName>
            <ecNumber evidence="3">3.2.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_19581</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.</text>
            <dbReference type="EC" id="3.2.1.22"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyl hydrolase 27 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.22" evidence="3"/>
    <dbReference type="EMBL" id="DS178423">
        <property type="protein sequence ID" value="EFP93981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338400.1">
        <property type="nucleotide sequence ID" value="XM_003338352.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LBQ6"/>
    <dbReference type="STRING" id="418459.E3LBQ6"/>
    <dbReference type="GeneID" id="10535594"/>
    <dbReference type="KEGG" id="pgr:PGTG_19581"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19581"/>
    <dbReference type="eggNOG" id="KOG2366">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032238_0_0_1"/>
    <dbReference type="InParanoid" id="E3LBQ6"/>
    <dbReference type="OMA" id="QEDYQIT"/>
    <dbReference type="OrthoDB" id="5795902at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004557">
        <property type="term" value="F:alpha-galactosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14792">
        <property type="entry name" value="GH27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.1180:FF:000097">
        <property type="entry name" value="Alpha-galactosidase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000400">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1180">
        <property type="entry name" value="Golgi alpha-mannosidase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002241">
        <property type="entry name" value="Glyco_hydro_27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013780">
        <property type="entry name" value="Glyco_hydro_b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041233">
        <property type="entry name" value="Melibiase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11452:SF75">
        <property type="entry name" value="ALPHA-GALACTOSIDASE MEL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11452">
        <property type="entry name" value="ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16499">
        <property type="entry name" value="Melibiase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17801">
        <property type="entry name" value="Melibiase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="7">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174151" description="alpha-galactosidase" evidence="7">
        <location>
            <begin position="27"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha galactosidase C-terminal" evidence="8">
        <location>
            <begin position="499"/>
            <end position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP93981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="596" mass="66096" checksum="9A2E97810619B41C" modified="2011-01-11" version="1">MLTIFQGSSLAILLAVAINHLGEVSAEVPTPSPTKPGPTSPASPNQVSGTYFGWSSWSLQAYKGKGYGSDWLTENNIKAQADVLSKEFSQLGYTRINLDSGWQDAELDKYGRTILNRKTFPSGIDNLSSYLAQKKLKLGLFYLPGIDSRAVNSKSVVMDTKYTADQIIQCPDKDALGCNRNTVNAFNAGMALNYSHPGAQMYINSVVDGLYSWGVSYVKLAGIVPGSMVDPPEYWKYNTTADLMAWRKAINELYEQKWQKQGRERIWLGASWKIPTSAGATMDKYVDSFRVEQDIEAYSETQMTTFDRVIRNAKTAALWSSVDPNRKWKGVRDLDSILISDMTLAECKTMVTIWAMFGSVLYLGDDLTKLPEVRKALVKNPEVLKLAILASTNEARLDGFNPSPSSAGSSSLKSSTMSLKSFEGLESKAIEEVQSVSSRDCDEMAEKRRLVVEKGLFQKVDDSDQLHETCMKKSKSAKSRLQARAFPSGAEDAQADQWSLQYWVLEQGQGDIYVAVVNAGQQTPDDKPVKVEISLSKLQTLQKSPRNKQMTGMKYTVRDLWNSKDLSSVAFEGKIESTLELHGTVLLKFSPVSHPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3LC01</accession>
    <name>E3LC01_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_19979</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178436">
        <property type="protein sequence ID" value="EFP94076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338495.1">
        <property type="nucleotide sequence ID" value="XM_003338447.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LC01"/>
    <dbReference type="GeneID" id="10535772"/>
    <dbReference type="KEGG" id="pgr:PGTG_19979"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19979"/>
    <dbReference type="HOGENOM" id="CLU_2134783_0_0_1"/>
    <dbReference type="InParanoid" id="E3LC01"/>
    <dbReference type="OrthoDB" id="10551742at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP94076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="113" mass="12997" checksum="3681E19351E610C3" modified="2011-01-11" version="1">MYLADRALVLDYLIKKIIPDRELFMRPWIVDYQVTSVVKQISNEKQKGKSGLPETFRPLNTKVSIHAIVMLERQRDLYLKNRDPRGVELLSCQAVGSWLKGWCFEGCLQVGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3NX97</accession>
    <name>E3NX97_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_20096</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS989924">
        <property type="protein sequence ID" value="EFP94196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338615.1">
        <property type="nucleotide sequence ID" value="XM_003338567.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NX97"/>
    <dbReference type="GeneID" id="10527288"/>
    <dbReference type="KEGG" id="pgr:PGTG_20096"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20096"/>
    <dbReference type="HOGENOM" id="CLU_054434_0_1_1"/>
    <dbReference type="InParanoid" id="E3NX97"/>
    <dbReference type="OMA" id="MQKIELM"/>
    <dbReference type="OrthoDB" id="2508351at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481:SF7">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="239"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="247"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="247"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="286"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP94196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="303" mass="33145" checksum="5AC27F4048608044" modified="2011-01-11" version="1">MPAWTVQNPSALIGNVPVLDGNSVTFPAWRTQLEDLLAIQGVHDIVTGKLPRPETDQKEAKPIAQGSQRVYFAEESGTDWDSLSEVARATMKMTLSVDLAIRYKDLKPASLLFKTICDAYEKNTRARRMMLEDSFWCARHDSSQPIAKWIAKIRNAASDLKSVKLTPADQQICDRLLRGLDNTWKPIRDHLVYSPNEISLDDAIGALEAHKVSMQVTFDHSGDNYAAAAVAKPKKRLGCWNCGQTGHHSSACPNPSLKNKSNATAKSAVVRAGATSAAPLGNGGPSDDEDDDSFDEEIDVVWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3NY85</accession>
    <name>E3NY85_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="5">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_20490</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="5"/>
    <dbReference type="EMBL" id="DS990054">
        <property type="protein sequence ID" value="EFP94534.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338950.2">
        <property type="nucleotide sequence ID" value="XM_003338902.2"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3NY85"/>
    <dbReference type="GeneID" id="10527910"/>
    <dbReference type="KEGG" id="pgr:PGTG_20490"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20490"/>
    <dbReference type="eggNOG" id="KOG0351">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003526_0_0_1"/>
    <dbReference type="InParanoid" id="E3NY85"/>
    <dbReference type="OrthoDB" id="10261556at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF145">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="701"/>
            <end position="856"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="889"/>
            <end position="1034"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="11"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP94534.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1332" mass="147646" checksum="683B41A8C3719CC6" modified="2012-04-18" version="2" fragment="single">YSAPEPEAIPVTNTPPSSNHPNSTLLAWDQLASALDNLDILGHKESISVTAQDNHTTPESCFMNCSGIYAHITACTNLLQCSIIELYRKVHYPMLLANWKPWVEKWLINCMTELHSVHDAVRMEALRGFIDHPPLRGLIQPLNHQSTVIQYASIAAEFIIFTLQTACQPLATHFWSDGDSQLATSVKKAASLLPLIQCADLNSEQEFSIILNTLFWQSVGFSCSIRKRSKVQSALEQFIALSTIKPDGSFCPPSDLTQTIAALQYTIRVATIFVCLRATELFAENPPDGFDPHNPHALDCASIHVFFRYFHDEKLISPFFTISNWMRLASSSEFDEDLPISSYWANSEMTQLVIGPTTITIAAVQRALRIAISSINGVIHTTLHGALFPAFPHSKLMDMAENTSVGFNYLVAPVNMPIHHFKLLQDWMLNHSSYGIAPPAWALAVSFKDGQFSVNVDNTCSRKHAWDWLERADLILEHIYFIYRVGCGLPDCRTEEAATLIRNLHSAPRNIYWRENRFLFQTYHHNRPSQVYLTTDLSMHLHNYLAYIRPVQMALNQHETANSMLSSLWVGSRQGTWELAQFDQILQNQFFLAGLKRIGLCAWRQASVSIAHAHLATKIPASLISTEDGLVKPTNANFLNASQAWQAYWGIESHLGFQKALLPVPNTLAPAKKAQQALAIFTQDPNASFRDDFQGEWLSCLFNDSNPADLLVVAKTGGGKSLAYLLPPLINPKERLVIIQPLKALVDQTVNDFKKLKTVTVQHFEKGNFIDPLAQVIIVTTNQAADSHFCNQLRSCLPTRIIIDEAHSFLEDGYRGYMPGVTALTQLSTQLILTTASLPHSQEDDLLRITFGLRRLITKRQPTFRPELNIEVLRSPAKWEQLSSVIQPLIDSFLVGPNDRAMIFIENKHAVELVGPQIGAVIHHSDLPDNVAASAVEKWTSGGARSIVATSGFGTGINYLHVRLVCIYGIPNRATANKAYQQLGRAGRDGHQAHIFFIPTVSDPHGDIDDFKKNLMNPAVCPANTFAQHQDNLNWSCRNFPTFLSCVCCSRLSANPALLPSTLSLSQSSSIPDWSPDPSAPSNSYNSSSTVSALATLGAHVGSGLAQSRKRRRLEDTDPLADKLATSNTLAAESSLSITSLARSAEQARLQSMPDNRVNSNQINCDNSLISHELVILQKFIRKMKDACGDCYVWNNTITPAHPRQCKPMHGKCLRCRAGKGWANHTHSGTEHTTKNCSSTSFSDSISEGSKGAHGFNICTRCGLPAGTQDQVNFHPNGNVGPNCDSGFANIAEAICWYVYRAQRPELGKWFSSAPLDSEPAFSKWIGITSKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPS8</accession>
    <name>H6QPS8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_20927</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EHS64315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890471.1">
        <property type="nucleotide sequence ID" value="XM_003890422.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPS8"/>
    <dbReference type="GeneID" id="13542592"/>
    <dbReference type="KEGG" id="pgr:PGTG_20927"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20927"/>
    <dbReference type="HOGENOM" id="CLU_174213_0_0_1"/>
    <dbReference type="InParanoid" id="H6QPS8"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="57" mass="5923" checksum="073D45971FB07E82" modified="2012-04-18" version="1">MLLTANVDHLLYQLASQASSSLLPAPMARKQFTQPCLQAFPAKPPIGVVPSDGGSAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQQ7</accession>
    <name>H6QQQ7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21237</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EHS62775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890192.1">
        <property type="nucleotide sequence ID" value="XM_003890143.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQQ7"/>
    <dbReference type="STRING" id="418459.H6QQQ7"/>
    <dbReference type="GeneID" id="13541313"/>
    <dbReference type="KEGG" id="pgr:PGTG_21237"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21237"/>
    <dbReference type="HOGENOM" id="CLU_2892193_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQQ7"/>
    <dbReference type="OrthoDB" id="2510873at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.50">
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS62775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="63" mass="6903" checksum="56E40DFB5F6161B0" modified="2012-04-18" version="1" fragment="single">MSIQPAFMNDSSTSTFVAAFCLQFFNAFNHDCASLMDVYATQYSFSLCASPYIPARAKMAGLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>H6QR00</accession>
    <name>H6QR00_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21343</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EHS62976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890059.1">
        <property type="nucleotide sequence ID" value="XM_003890010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QR00"/>
    <dbReference type="GeneID" id="13540909"/>
    <dbReference type="KEGG" id="pgr:PGTG_21343"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21343"/>
    <dbReference type="HOGENOM" id="CLU_2590905_0_0_1"/>
    <dbReference type="InParanoid" id="H6QR00"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS62976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="80" mass="9012" checksum="63FD8F0B4E5FD39A" modified="2012-04-18" version="1">MAQSLPQHTEAEEGDKEELEVAIKKAPKAFNGEEPLSNNQLNLINMLIFSKKVYMNANESIQKQILFGSGREFHVMVPLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>H6QR85</accession>
    <name>H6QR85_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21407</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EHS63067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889997.1">
        <property type="nucleotide sequence ID" value="XM_003889948.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QR85"/>
    <dbReference type="GeneID" id="13540507"/>
    <dbReference type="KEGG" id="pgr:PGTG_21407"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21407"/>
    <dbReference type="HOGENOM" id="CLU_1636228_0_0_1"/>
    <dbReference type="InParanoid" id="H6QR85"/>
    <dbReference type="OrthoDB" id="10268798at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="162" mass="17758" checksum="3399879C2F58AFD0" modified="2012-04-18" version="1">MINRSFKSQMDDPHPKAEKSGRIGGSSVRIESVKLYGVPEVGETGHENGSVMCCTHHRKGEHTVGGIHHPPDGQPNRKTSGWRNVRAWGSYRSSNRTRRPYHVVGPSAEEILPTNNTGSTFQKLADKSYRLNSPKSSATLKNLDPKNHTGPTFYLADGGIPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRE0</accession>
    <name>H6QRE0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21357</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EHS63206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889913.1">
        <property type="nucleotide sequence ID" value="XM_003889864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRE0"/>
    <dbReference type="GeneID" id="13540563"/>
    <dbReference type="KEGG" id="pgr:PGTG_21357"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21357"/>
    <dbReference type="HOGENOM" id="CLU_1143022_0_0_1"/>
    <dbReference type="InParanoid" id="H6QRE0"/>
    <dbReference type="OrthoDB" id="10659853at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="116"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="243" mass="25849" checksum="F6DDDF7C976DFD95" modified="2012-04-18" version="1">MSVVVSSDSESEYRDSDHSPLSAPDPPGPNTRVTTHSSNRDSRTGAVRPTVRRPNGGYSDQSPSPAPDTPGRHTRVTTRSNTREAQTGPIRPTARQTHGNGGRVPARPQRGASTTSAAPPGRGASRPRPANLPAVPSRARAPASSTQQATASQAPGNEADPQGAWPPDTVHLTLDDEPTNDEFVSEVDAIFQLGGPYADLGEQLAYLCTFAKAICVSHRDAKMVNLLATRGAKLKMLEFELWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRW0</accession>
    <name>H6QRW0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21476</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EHS63402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889771.1">
        <property type="nucleotide sequence ID" value="XM_003889722.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRW0"/>
    <dbReference type="STRING" id="418459.H6QRW0"/>
    <dbReference type="GeneID" id="13543066"/>
    <dbReference type="KEGG" id="pgr:PGTG_21476"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21476"/>
    <dbReference type="HOGENOM" id="CLU_1548364_0_0_1"/>
    <dbReference type="InParanoid" id="H6QRW0"/>
    <dbReference type="OrthoDB" id="444135at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008220">
        <property type="entry name" value="HAT_MetX-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268">
        <property type="entry name" value="HOMOSERINE O-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32268:SF16">
        <property type="entry name" value="SERINE O-SUCCINYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="173" mass="19191" checksum="6CCFDDBA4DC20223" modified="2012-04-18" version="1">MCAGFRRLPSPALSLPVSRPSTPSFFESNNSRLYVPGRSPIRRAIHVQSTAKRFQLDAKDSPVTVYRSYSKHPSPRAPDGPEPTYSKLVSGYQIYHHSTPFKLDYGHSLPSFDLAYEVWGKLSDDKDNVILLHTGLSASSHAKSHQENRKSRNVGKIPLATAQAIRSTSTVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>H6QS06</accession>
    <name>H6QS06_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_21573</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EHS63441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889726.1">
        <property type="nucleotide sequence ID" value="XM_003889677.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QS06"/>
    <dbReference type="GeneID" id="13543103"/>
    <dbReference type="KEGG" id="pgr:PGTG_21573"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21573"/>
    <dbReference type="HOGENOM" id="CLU_659115_0_0_1"/>
    <dbReference type="InParanoid" id="H6QS06"/>
    <dbReference type="OrthoDB" id="6513042at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003605764" evidence="2">
        <location>
            <begin position="21"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS63441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="417" mass="45080" checksum="C1C147BD13AFDF2C" modified="2012-04-18" version="1">MISIYVSAFVICLFQLGVSAGPPTPSENVLSISVTPIYANSSALLPQSTLGPADGIFRRQTTTPRLLTQNMNNPSTVVSALNNTNNRPANSTGARANDNNRNPKTFTETVRISGGLVRTQASWIDGLKIDAISATPHTLSIKKKTNPAASAKFVTSADPSRNILGGEWRELTSFSYDVSFEGEYPDDLSFKISAPYDKLQAPITVGLDDEVYLGLYDPNRSGWVVDTERMENRRKDKRVDLIGIPAPTGEYRLLARSNRDPLGSMALSFGTGTDGQFNVLAPSGGLSSTPPLQVATWQDGSKIIIRSLQAMQIKMETSNTTTSAIPPGYEAATRYGFTITTNTPAAQVIMNLQLPYVPTQLEARGLFPQDLVTAGRPLRANQQYQILSATTISGNGALMNVPMNIVEGEYLLLARSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>H6QS09</accession>
    <name>H6QS09_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21576</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EHS63444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889729.1">
        <property type="nucleotide sequence ID" value="XM_003889680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QS09"/>
    <dbReference type="GeneID" id="13542811"/>
    <dbReference type="KEGG" id="pgr:PGTG_21576"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21576"/>
    <dbReference type="HOGENOM" id="CLU_2528522_0_0_1"/>
    <dbReference type="InParanoid" id="H6QS09"/>
    <dbReference type="OrthoDB" id="2509327at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="84" mass="9341" checksum="AE4A17E9550051C0" modified="2012-04-18" version="1">MARSGTRQCFLPTLRATMAKSGREKYRDNTTAEERSTKLESGTSDFTATMYEQPTPGSESPVWPGLARRRLQKASSLTSVDRPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>H6QT11</accession>
    <name>H6QT11_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21916</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178303">
        <property type="protein sequence ID" value="EHS63962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889359.1">
        <property type="nucleotide sequence ID" value="XM_003889310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QT11"/>
    <dbReference type="GeneID" id="13540751"/>
    <dbReference type="KEGG" id="pgr:PGTG_21916"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21916"/>
    <dbReference type="HOGENOM" id="CLU_040957_2_0_1"/>
    <dbReference type="InParanoid" id="H6QT11"/>
    <dbReference type="OrthoDB" id="2505029at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="212"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="212"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="246"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="260"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="309" mass="33752" checksum="E7806628E63A2991" modified="2012-04-18" version="1">MAATRAPNHPAFFNGHFQPISESVADSVRANQYGYVKTPSFLQCAGSNGDKNEDFEVQLITNTALNNTLNSESIYALSGKFIALNNGSTPVLSYIQETVIRIGTPGPNQPDFTNKTVVTSLGMVVSRREVATQTSDSATQLEVIIAHCDWDGEDRVHRRFNIKYIVPGTKNLVKTHTLYQVGREVYIIGRLVDFHMDENIAVVVFLPKAGLSPSSPTTASLPKPKLTKSPATQIPSVTNGPPPKQTLDKDNKGKAKAVDESETDDDDGSNDNSDEESEVDTEAGPNIKAKRGCPRKTIIQEALKRMKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTB4</accession>
    <name>H6QTB4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22026</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178310">
        <property type="protein sequence ID" value="EHS64068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889247.1">
        <property type="nucleotide sequence ID" value="XM_003889198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTB4"/>
    <dbReference type="GeneID" id="13541679"/>
    <dbReference type="KEGG" id="pgr:PGTG_22026"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22026"/>
    <dbReference type="HOGENOM" id="CLU_3033447_0_0_1"/>
    <dbReference type="InParanoid" id="H6QTB4"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="55" mass="6043" checksum="001461AF68DF8D8B" modified="2012-04-18" version="1">MEVSVMDRSEVSGQGNYLSSLLKKVDWTAEYVVAEGSLWVDGMESGHRSNHSIGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTG3</accession>
    <name>H6QTG3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22088</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178313">
        <property type="protein sequence ID" value="EHS64178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889194.1">
        <property type="nucleotide sequence ID" value="XM_003889145.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTG3"/>
    <dbReference type="GeneID" id="13541164"/>
    <dbReference type="KEGG" id="pgr:PGTG_22088"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22088"/>
    <dbReference type="HOGENOM" id="CLU_045723_0_0_1"/>
    <dbReference type="InParanoid" id="H6QTG3"/>
    <dbReference type="OrthoDB" id="2498658at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="348" mass="40857" checksum="8900C8FE3A26C65D" modified="2012-04-18" version="1">MEDLNSREIETIGIEYIEQLTKLLASIQANFIPEYEFNFLNLERLTCETIYYFYKHELITRPNLGILLTNEDASKLFIKHILQSYLYDQKKTRNWVPLNAKNILNHWLHFSWGNMFEGLPEVFKNIFSYNLNKAVFEAYQAYPQERKNERLKWMMDGIKNLVFSKIPTRPENLLNASGNSRTPIVTVHTTSLMELVKAFITIHKDPKSPSELSHLFQAIEYYSKANPNLFAIPQRTSSTFKRKRDLMSKSGEILAEIENLQLYLSNKFNQSRWLNSIFVQPSNNFKSVSHLEELRILACYIKRIEEDYERRIGVMLQSNQFHQYCQVQLVTNSLNAVKAMLKTIAESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTV4</accession>
    <name>H6QTV4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22188</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178322">
        <property type="protein sequence ID" value="EHS64364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889074.1">
        <property type="nucleotide sequence ID" value="XM_003889025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTV4"/>
    <dbReference type="GeneID" id="13541797"/>
    <dbReference type="KEGG" id="pgr:PGTG_22188"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22188"/>
    <dbReference type="HOGENOM" id="CLU_2759023_0_0_1"/>
    <dbReference type="InParanoid" id="H6QTV4"/>
    <dbReference type="OrthoDB" id="10374982at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="70" mass="7946" checksum="F87F54B3D09E1CBB" modified="2012-04-18" version="1">MDIAAHYNLFRTVYDCRECRSELTRSQVSEEAILALKLVRPSSQASHIRDLALIWGTQDTTAISAMMRGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUT4</accession>
    <name>H6QUT4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22521</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178354">
        <property type="protein sequence ID" value="EHS64842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888729.1">
        <property type="nucleotide sequence ID" value="XM_003888680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUT4"/>
    <dbReference type="GeneID" id="13542095"/>
    <dbReference type="KEGG" id="pgr:PGTG_22521"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22521"/>
    <dbReference type="HOGENOM" id="CLU_047861_0_0_1"/>
    <dbReference type="InParanoid" id="H6QUT4"/>
    <dbReference type="OrthoDB" id="3046222at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="331" mass="38804" checksum="5A5B11531424D604" modified="2012-04-18" version="1">MKNPKAGAFKLKIGQPNGSEDPGQSVSKIHPSLMNSDRLRYYRKLVLTEMGYVPERHGAGVGDKFITDMFHWTERGLKIISCSFLENKEHFTFQTNKMHDRLLARGKDREVYQGGLLSDVTYRFFENGCLLTTPIFCEQLTRWIPVQLSWIRGLSENYYQIHFTILFRQFMIPSLLPSKRETLARQVVDFSMAQKEGFVGAFMEVFGKADWTRALRNLKGCHEHFQAQITRIRRNRTIVSVEQEANFERMCSALLERSQPGGTSHEEKIDKLRRRFPKAKRWLDWWTATDVEAMLFPSRRAMLDDYPEGEDNLPDTKNAQESMHRLYYMIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>H6QVF2</accession>
    <name>H6QVF2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_22731</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178407">
        <property type="protein sequence ID" value="EHS62906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888513.1">
        <property type="nucleotide sequence ID" value="XM_003888464.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QVF2"/>
    <dbReference type="GeneID" id="13540835"/>
    <dbReference type="KEGG" id="pgr:PGTG_22731"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22731"/>
    <dbReference type="eggNOG" id="ENOG502T1DR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054434_1_0_1"/>
    <dbReference type="InParanoid" id="H6QVF2"/>
    <dbReference type="OrthoDB" id="2503412at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003605834" description="CCHC-type domain-containing protein" evidence="4">
        <location>
            <begin position="24"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="5">
        <location>
            <begin position="140"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="147"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS62906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="205" mass="22784" checksum="92D389E1B12E48D2" modified="2012-04-18" version="1">MPCIWFLFFVAQLVSVQVHRSEAYKKNTRARRLMLEDAFWSAKHDPNVPIAKWIARIRNAAFDLTSVKLTPNDQQVCDRLLRGLDNSWKPIRDHIVYSPNEVSLDDAIGALESHEVSTQVSTDHFDASAAATKAKPKLGCWNCGQKGHHSSKCSNPSLKKKPTAQASSTEARAGAVSYATLGNYSEDEENDDNSYDDDGCDVVWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQJ1</accession>
    <name>E3JQJ1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phytocyanin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_00638</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307688.1">
        <property type="nucleotide sequence ID" value="XM_003307640.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQJ1"/>
    <dbReference type="STRING" id="418459.E3JQJ1"/>
    <dbReference type="GeneID" id="10527984"/>
    <dbReference type="KEGG" id="pgr:PGTG_00638"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00638"/>
    <dbReference type="HOGENOM" id="CLU_1180460_0_0_1"/>
    <dbReference type="InParanoid" id="E3JQJ1"/>
    <dbReference type="OMA" id="SEDPIWY"/>
    <dbReference type="OrthoDB" id="1921208at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00920">
        <property type="entry name" value="Cupredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052953">
        <property type="entry name" value="Ser-rich/MCO-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34883:SF15">
        <property type="entry name" value="EXTRACELLULAR SERINE-RICH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34883">
        <property type="entry name" value="SERINE-RICH PROTEIN, PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172878" description="Phytocyanin domain-containing protein" evidence="2">
        <location>
            <begin position="18"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="152"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP74682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="236" mass="23717" checksum="4CBA0746EF2B167A" modified="2011-01-11" version="1">MNTQLLTVFALVGVAMASDFRVTVGISQITGQVGTGLDPNRIVPAAGDTITFSWLIPSYIQNPATGTYSVTQGTYDAPCKPMAGGFDSGPKTTGPESAGQAPTMTFQVKDDKPLYFFSSVDDQCKKGMVLGVNSPASGPGSVESYILAAGGNPNADTTTNATTTPAAPSSTNTTSASNTSASKNNTSPSSTTASKQPANSGSTNSSSAKPQGSTANTVAPQSLLTAAGVLSAALFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRD8</accession>
    <name>E3JRD8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00558</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307608.1">
        <property type="nucleotide sequence ID" value="XM_003307560.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JRD8"/>
    <dbReference type="STRING" id="418459.E3JRD8"/>
    <dbReference type="GeneID" id="10528076"/>
    <dbReference type="KEGG" id="pgr:PGTG_00558"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00558"/>
    <dbReference type="HOGENOM" id="CLU_012390_4_0_1"/>
    <dbReference type="InParanoid" id="E3JRD8"/>
    <dbReference type="OMA" id="YTETRFR"/>
    <dbReference type="OrthoDB" id="124998at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47150:SF5">
        <property type="entry name" value="OS07G0546750 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47150">
        <property type="entry name" value="OS12G0169200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP74602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="143" mass="17048" checksum="98708F5E4127FD47" modified="2011-01-11" version="1">MSNHIGNRRRRMEELYDWDMEDEDEDSINIFKELETEEEEEKSRAQRRPNKERSHLEGHQRLLQDYFVQGSTYSDTDFKRRFQMRRELFLKIVVDVEAACPYFVQKPDCTGKLGLSSLQKATSAVVIIPVFLRQKYKNLLLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRQ0</accession>
    <name>E3JRQ0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="8">ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_00499</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="6">Belongs to the Mrp/NBP35 ATP-binding proteins family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307549.1">
        <property type="nucleotide sequence ID" value="XM_003307501.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JRQ0"/>
    <dbReference type="STRING" id="418459.E3JRQ0"/>
    <dbReference type="GeneID" id="10527805"/>
    <dbReference type="KEGG" id="pgr:PGTG_00499"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00499"/>
    <dbReference type="eggNOG" id="KOG3022">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024839_0_2_1"/>
    <dbReference type="InParanoid" id="E3JRQ0"/>
    <dbReference type="OMA" id="CNHESHI"/>
    <dbReference type="OrthoDB" id="1741334at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032981">
        <property type="term" value="P:mitochondrial respiratory chain complex I assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001278">
        <property type="entry name" value="Iron-sulfur cluster carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044304">
        <property type="entry name" value="NUBPL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961">
        <property type="entry name" value="IRON-SULFUR PROTEIN NUBPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42961:SF2">
        <property type="entry name" value="IRON-SULFUR PROTEIN NUBPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="13"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="314"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="32"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00024036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP74543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="362" mass="38932" checksum="C13DDABFC68CB0D9" modified="2011-01-11" version="1">MLKLPSSAFLRRPRLQPGLLGRTSRGSRDLQTHTFCSSGPSRSHDNPLGLPAQQAGLPRSGSPPKLNISRSLPTKRDIPNVRKVLVVASGKGGVGKSTVAVNLAIAIRNQSALNVGLLDLDIFGPSVPKLMGLDEGLSPELTDQNALVPLRNHGISCMSIGFLIPPSESPDSVVAWRGMMVMKAVQQLLFDVDWRGRAGEEEGPGLDILVIDMPPGTGDVALSLGQLVNIHGAVIVTTPQDIALIDVTKGVNMFRKLNIPIIGSVLNMSSFKCTKCETKHEIFGPMTSFKRVLERNNVELLGQVPLDLQISKSSDAGQPISISKRPPPEHPEPPDANTPTSASFDDSPFIEISRKCLDHLLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3JSC7</accession>
    <name>E3JSC7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_01445</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP74852.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319271.2">
        <property type="nucleotide sequence ID" value="XM_003319223.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JSC7"/>
    <dbReference type="GeneID" id="10546968"/>
    <dbReference type="KEGG" id="pgr:PGTG_01445"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01445"/>
    <dbReference type="HOGENOM" id="CLU_015326_0_0_1"/>
    <dbReference type="InParanoid" id="E3JSC7"/>
    <dbReference type="OrthoDB" id="10680768at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171494" evidence="2">
        <location>
            <begin position="26"/>
            <end position="747"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP74852.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="747" mass="85829" checksum="3E9C43D8928A01A1" modified="2012-04-18" version="2">MSSAHHLWLLRVNFLLIVYLKLSICSPPTPIPAELEVPTIGALEHRPDSSVDRSESWELLAFQSARKSRKVSSDYDSVGGRAMKNFDLNEEYRGQWDESELDSSGNGSRHHDTVSSAIGDGANSKDKTFPSQSDSPTASNSLDSQHGRQVEISTVSKKRSRDDQLANSEFRHPTQTMQEGKMTAQPESHSPSLLNHADQGQQPIPSSSGHSPIKFEMLPYDERWHPFFDWVKRYFFQRNSELQSESIKRFLRRLEKEQVTLNEMLPNRKYYKKVSSKDISLEIIHVDEDPDIEDFHDFFFLPKGIRGAQARRGKCLINIRVRVTKILEAAELFIGLASLQEIDPVIFGPQWENTRQEFMEWIEKLFFEATEDSLPIFGQYSNTWTEAERAANRFGDAQKALAMVLVHQKKHTDVQQTKQRHNEIYSVSLSLLEYWYKISALKLGRSDPKGHPENLWIFAESVSFWKDDLSRLMKSGIGASKNQSRPQDNVVTTDHQIESPELDFFSAKIKEHFGNSGDIQHKESLFTVVSRHQKKGESMITQGIRAFIRSELEKSVLALQDHFSRINEPIKVTDLPIYLIPAYHHDHTLETRHVFINLSDGRDGGRTRTDQVTLFESRILKILESLSILCQIASVNLGVDDPQKTHQILIDWYLASLFKHSAQDGLPLFGWVQIVFPPIHPPEELFGIPQKFLSKELTTSRPLHRKQAHKLAFLLFSFWYEEVAVKRGLFGPIDDQLLSFGELWQHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3JSQ5</accession>
    <name>E3JSQ5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01673</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75080.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319499.2">
        <property type="nucleotide sequence ID" value="XM_003319451.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JSQ5"/>
    <dbReference type="GeneID" id="10547442"/>
    <dbReference type="KEGG" id="pgr:PGTG_01673"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01673"/>
    <dbReference type="eggNOG" id="ENOG502S2AH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042437_0_0_1"/>
    <dbReference type="InParanoid" id="E3JSQ5"/>
    <dbReference type="OrthoDB" id="2506566at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="270"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75080.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="329" mass="37740" checksum="493263488F7F98E9" modified="2012-04-18" version="2">MPFHPVKLTAVQLHGERQPYLDSKRIGTRLGTELKERIMKAMQNRQSAVKSLLDSFNELLGEFIEKFPNQQACDSSLYPLEYRTFSQWPLDHHFWNDGLYMRCKEPWGIDPNVRAGINCVLILSRVQEEFQLIAQELARAIGWAVAYYSRISDRITYISRRIGLLANETNTSEDIELDYIDNLELCGLSRLDKLRVIRKILRHHRSTHATLVGEWHSHVMWLCSHCQPANNRIALQQQWQQMRHEIRRDQAESVVVNTGEVDAALEEGVLEVGQDDGEDADGNWMTDEELEDNQEEQDQDLNTEESPAHTGDAGVSAEVGDLEQSSSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3JTF4</accession>
    <name>E3JTF4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADH dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01821</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319647.1">
        <property type="nucleotide sequence ID" value="XM_003319599.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JTF4"/>
    <dbReference type="STRING" id="418459.E3JTF4"/>
    <dbReference type="GeneID" id="10542602"/>
    <dbReference type="KEGG" id="pgr:PGTG_01821"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01821"/>
    <dbReference type="HOGENOM" id="CLU_166990_0_1_1"/>
    <dbReference type="InParanoid" id="E3JTF4"/>
    <dbReference type="OMA" id="EKPAIFW"/>
    <dbReference type="OrthoDB" id="2093409at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22903">
        <property type="entry name" value="NI9M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039961">
        <property type="entry name" value="Nuo9.5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38488">
        <property type="entry name" value="OXIDOREDUCTASE 9.5 KDA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G08980)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38488:SF1">
        <property type="entry name" value="OXIDOREDUCTASE 9.5 KDA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_5G08980)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="28"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="82" mass="9348" checksum="7F0A6F0DC0B73207" modified="2011-01-11" version="1">MASASVVRLGRFQKVRRYLQYQAHENPAIFWSVALGTAGPVLLATVPPIRRNYFGYVTPEPIPMSYPLPQRKRNPDLKGYDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3JTQ6</accession>
    <name>E3JTQ6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00788</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75457.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319876.2">
        <property type="nucleotide sequence ID" value="XM_003319828.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JTQ6"/>
    <dbReference type="GeneID" id="10543087"/>
    <dbReference type="KEGG" id="pgr:PGTG_00788"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00788"/>
    <dbReference type="HOGENOM" id="CLU_046891_0_0_1"/>
    <dbReference type="InParanoid" id="E3JTQ6"/>
    <dbReference type="OrthoDB" id="2503565at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="278"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75457.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="309" mass="34580" checksum="65B6F8716F905CD9" modified="2012-04-18" version="2">MSTPSDKTSKPSSKSHDPPPHLSPAMSTDLTGHPTLKITSIEKLKPPGPDSNYLDWSWVLDIHFDSTGVSYILDPKEANPEAKPTFARDNKAICSVISQTINSANIRVVRQFNKDTRKMWNGLRAAHQDSSSGGVMYWLRKLTMFRMENDDIETHLEEMAKVFERLEALADPARPLTVDDFYSSSIITSLSAEWMPCVSSLLNEPHVPSHKVVAALKQEGLRRKARIEDLQAPESVSKTTTSLSNRQRRTARHCTYCGNDGHDLNYCHKLARFIEDRNSTGKERVPAPSGSLIANPPSSRGRYNKTHQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3JTX5</accession>
    <name>E3JTX5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">sterol 3beta-glucosyltransferase</fullName>
            <ecNumber evidence="2">2.4.1.173</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Autophagy-related protein 26</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_00860</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a sterol + UDP-alpha-D-glucose = a sterol 3-beta-D-glucoside + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22724"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15889"/>
            <dbReference type="ChEBI" id="CHEBI:37424"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.173"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:22725"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ergosterol + UDP-alpha-D-glucose = ergosteryl 3-beta-D-glucoside + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61836"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16933"/>
            <dbReference type="ChEBI" id="CHEBI:52973"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:61837"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 28 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.173" evidence="2"/>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75529.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319948.2">
        <property type="nucleotide sequence ID" value="XM_003319900.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JTX5"/>
    <dbReference type="GeneID" id="10543245"/>
    <dbReference type="KEGG" id="pgr:PGTG_00860"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00860"/>
    <dbReference type="eggNOG" id="KOG1192">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000537_8_5_1"/>
    <dbReference type="InParanoid" id="E3JTX5"/>
    <dbReference type="OrthoDB" id="10261837at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016906">
        <property type="term" value="F:sterol 3-beta-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000029">
        <property type="entry name" value="Sterol 3-beta-glucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000009">
        <property type="entry name" value="Sterol 3-beta-glucosyltransferase UGT80A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010610">
        <property type="entry name" value="EryCIII-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050426">
        <property type="entry name" value="Glycosyltransferase_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004276">
        <property type="entry name" value="GlycoTrans_28_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48050">
        <property type="entry name" value="STEROL 3-BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48050:SF25">
        <property type="entry name" value="STEROL 3-BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06722">
        <property type="entry name" value="EryCIII-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03033">
        <property type="entry name" value="Glyco_transf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase family 28 N-terminal" evidence="8">
        <location>
            <begin position="32"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="Erythromycin biosynthesis protein CIII-like C-terminal" evidence="9">
        <location>
            <begin position="340"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP75529.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="513" mass="58245" checksum="34E9023F2325A58F" modified="2012-04-18" version="2">MLPPIQDIKMPIELSMPLAMGFQTRSPRSMKITCLTIGTRGDVQPYIALCQRLNQLGHICTIATHKNFRDWIESSGILFAEIEGDPEELIEHCVEFGVLSPFFWWNGLSRFTPWFDGLLTSSWKACQGSDLLIESPSALIGVHIAQSLNIPYYRAFTMPWTKTGVYFHAMLAMSDTWGSWINRMSYILFDLLLWAILSSRINAWRESVLGLEPTTFSQLRLDQVPFLYNFSSFVVPRPDDWQPWVKTTGYWFLNSQENTSSEKYPENSIPEDLKKVIHQVKESGRKIVIVPDPEKIMNAIIGAVKESNVFAIVSGGWTAQQAERIHESSAMRAYINQHLDSMYYVDSIPHGWLFDQIDAALHHGGAGTTAASIKAGIPTMIKPFFGDQNFWARRVEELGVGTHLKTFEKQDILQAIRRATTDENQINQARILGEKIRRENGVDNAIKAIYEYLDYSQCLIETTRSLSSQSQPNQFSQVASAVKSKFSSTIKAVYFYRPATLSRSNYDAYSSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3JTY2</accession>
    <name>E3JTY2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_00834</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75503.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319922.2">
        <property type="nucleotide sequence ID" value="XM_003319874.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JTY2"/>
    <dbReference type="GeneID" id="10543133"/>
    <dbReference type="KEGG" id="pgr:PGTG_00834"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00834"/>
    <dbReference type="HOGENOM" id="CLU_627203_0_0_1"/>
    <dbReference type="InParanoid" id="E3JTY2"/>
    <dbReference type="OrthoDB" id="2496481at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="158"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="402"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="347"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="405"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP75503.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="437" mass="46952" checksum="267BF48B214C3E25" modified="2012-04-18" version="2">MPPSAPADKDKDTTNAASGQDNSPCHILTSYSLSTAIYYRPVPQAQVHTFIVPEAPTQAGPAPSQETAPPSILQKRNPWPDGPDDDSTVGTNKPKTRPASAAAASFPWKDVYAVPHVTSLGNCPTTATSPPQITLPSATAHPTSDHELNAAHKVNIHLAILVGGLTVGMIVITILIAVAGCIICTRRRKRRAHKGNQLSDVDDTLTCQSNNTAGSSRTTMSERHLKCQTGWQMKEFMHQGMHSPFSSTAMLPSPCDKVEILARTGTISDPFHDRSSNWGRSTHPAIYGNHPESLSDTCSTDAPSYFPGDDIRYTLPQGSTTALTEASCAATIDSTSNSVCPNRLNHPASQPSDSSSSPTVTANLNSLIKHKAFVSSMMSEPPFLQSFHSDALHPLYLSPPDPVITSKSRPQSSQSFADSVYSDSTHESMYWINRPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3JU15</accession>
    <name>E3JU15_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00871</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319959.1">
        <property type="nucleotide sequence ID" value="XM_003319911.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JU15"/>
    <dbReference type="GeneID" id="10543256"/>
    <dbReference type="KEGG" id="pgr:PGTG_00871"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00871"/>
    <dbReference type="HOGENOM" id="CLU_175738_1_0_1"/>
    <dbReference type="InParanoid" id="E3JU15"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="102" mass="11582" checksum="B30D708A3690FAC2" modified="2011-01-11" version="1">MNSTDHNPSANTKEPEDPTWQNRSLIDRLSSPIASTLSNKNLASEPTDKVATTKTSNNQNIRQHLKTDRGKNFTELSRRKETKTDQEPSSEQKLTILFNNEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>E3JU39</accession>
    <name>E3JU39_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Histone-lysine N-methyltransferase, H3 lysine-79 specific</fullName>
            <ecNumber evidence="2 11">2.1.1.360</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 11">Histone H3-K79 methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_00895</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Histone methyltransferase that specifically trimethylates histone H3 to form H3K79me3. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-lysyl(79)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(79)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60328"/>
            <dbReference type="Rhea" id="RHEA-COMP:15549"/>
            <dbReference type="Rhea" id="RHEA-COMP:15552"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.360"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="11">Ubiquitination of histone H2B to form H2BK123ub1 is required for efficient DOT1 methyltransferase activity on histone H3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="11">In contrast to other lysine histone methyltransferases, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the class I-like SAM-binding methyltransferase superfamily. DOT1 family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.360" evidence="2 11"/>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75564.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319983.2">
        <property type="nucleotide sequence ID" value="XM_003319935.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JU39"/>
    <dbReference type="STRING" id="418459.E3JU39"/>
    <dbReference type="GeneID" id="10543280"/>
    <dbReference type="KEGG" id="pgr:PGTG_00895"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00895"/>
    <dbReference type="HOGENOM" id="CLU_027817_0_0_1"/>
    <dbReference type="InParanoid" id="E3JU39"/>
    <dbReference type="OrthoDB" id="443402at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031151">
        <property type="term" value="F:histone H3K79 methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140956">
        <property type="term" value="F:histone H3K79 trimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000033">
        <property type="entry name" value="Histone-lysine N-methyltransferase, H3 lysine-79 specific"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025789">
        <property type="entry name" value="DOT1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030445">
        <property type="entry name" value="H3-K79_meTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21451">
        <property type="entry name" value="HISTONE H3 METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21451:SF0">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE, H3 LYSINE-79 SPECIFIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08123">
        <property type="entry name" value="DOT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51569">
        <property type="entry name" value="DOT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DOT1" evidence="13">
        <location>
            <begin position="316"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="35"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="329"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="50"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="70"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="105"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="160"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00029821"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00047770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU271113"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EFP75564.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="653" mass="73988" checksum="9E507B632D463477" modified="2012-04-18" version="2">MDCFFKDPRLLKQQQQQKQTTTQQQQQQIRIKTLQTNQQPLPKRIKLNHQQQTATGTNQTTTTTKKTIHSKPQLNNTQQPDQLSKTRETKPLVNQTEDNHHKTQDINNNNKTQDINQTQDINKTQDINKTQDINTTQNNNKTQDIKKTQDINKTQNNNKTQDKQHQDDQNQTKKNPTTNHQDYQLHPIIPRSVDKTITAYSSNHPLNVEPAERKLTSSAKLVNANRSQFKPWFTNQDPQRWPFKKNQDPQIRVVYPTGAHELFPLLVPKNDLDEYRPLDDLLNVITTTLTYYLTPDQSLHFFSEPHPITSPSASVPHSQLNDFSFLNRRSQERSSPEPGRSTTPKIPLSDASLDLSSKPLLKELAKTIRRKNGPEFLGLVGWYNRVIKYLVEGKEIGRQIGSFVGMPQGVWETILGQAYDRQVGPQLELLQGYETWSSNVYGELKPRFVSEIIRLVGLRPGMVFLDLGSGIGNIVLQVALEVGCVAVGFEIMDGCAKLANLQRSELVGRAHSLWGVNLGAPLLFQADFTKDPRVGQWLQQADVVLVNNQVFTPSLNESLSLLFLELKDTAQIVSLKPFISSSFKLNQRNLDSPLAILSRPALRPTPPSTLVANSPGVFSYPSNSVSWTDNPGDFFVSVVNRDKLIRFQESCQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3JU46</accession>
    <name>E3JU46_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00908</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00908"/>
    <dbReference type="HOGENOM" id="CLU_1778390_0_0_1"/>
    <dbReference type="InParanoid" id="E3JU46"/>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="67"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="146" mass="16226" checksum="22E8D356D263ABF5" modified="2011-01-11" version="1">MSTKGYGLIGSRLSIAFQKDLNLPNKFTTPPVHLDVQVAGSRGMAPGQPPPQIRASQARAPPKIKGQPWDVLVERPCDVPPKHPTDGQEVFEGRLLQNSKKLLQVTQSRQQDRDNCMVVLQCLQFSYRYEWGSGVLIPHSPPAMDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUC5</accession>
    <name>E3JUC5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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        <name evidence="2" type="ORF">PGTG_00981</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75650.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320069.2">
        <property type="nucleotide sequence ID" value="XM_003320021.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JUC5"/>
    <dbReference type="GeneID" id="10543450"/>
    <dbReference type="KEGG" id="pgr:PGTG_00981"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00981"/>
    <dbReference type="HOGENOM" id="CLU_032241_0_0_1"/>
    <dbReference type="InParanoid" id="E3JUC5"/>
    <dbReference type="OrthoDB" id="2424990at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="30"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
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            <begin position="86"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="107"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="274"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="355"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="372"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="417"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75650.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="575" mass="64417" checksum="A014390A62E14877" modified="2012-04-18" version="2">MVPNQSSGSTIETEPTQPHPGPVSSIGAPSVGNLSLGSGASSGFISSAANNSASQKKATARKRSTGRSQESRGIEDEMRIAEQRARIKRLKAGQKKRAGGSKNQNNSTATSSRSRKTTSQSRGRTARSESQSSDGRDTNPQFVQEDYENLCCYLEEEKNYNQLYGIQDKTEVGGTFLKKSAAYDVFAIYINDRSNNRLKLTGKQLRQRLDRYKARFARTKEWAENTGAGIEESDGANTLAAILEKRCPCYERMYAIFGAKHNVTPMAQYESTKGTGLYNSNSEQDSDMSDQAGDNETIARNMNNINSPEENHQADNNCLEGCMETNEGNECAMDVEHDVQSTRNGTHLESPDIELNDRDGEGVGSNDRDGEANELNDQDVEANPDQGLGEPDIRIESASERNQSPSTSPLRRGPLGSSMNANSADDSSQKSKSSVAGAFQDSTNKRFGFLESHLSWEKEQFRYMRAKEHRRIVAEEERDKQHLGWEREKYDRQEQQQQTKDYRSFQLEVVKLHQHSNEVQNRMALEKEHADLQRMKFDQEVKEKQERVDLAKQMMNDGKSAAEIESLIRVIYGTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUW0</accession>
    <name>E3JUW0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_01166</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01166"/>
    <dbReference type="HOGENOM" id="CLU_035530_0_0_1"/>
    <dbReference type="InParanoid" id="E3JUW0"/>
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        <location>
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        <location>
            <begin position="199"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
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    </evidence>
    <sequence length="528" mass="61419" checksum="E66714D9E91AFEEA" modified="2012-04-18" version="2">MPMMRYQAHCFLFVYAAIIAASGALDDMFNGHHFQSLGFGEQHTALDDLQHSISTLPPDQLNQPDDPSRYSSSSNSQPSFFDASDDFLQTEFLPHDPFETAWPPSPGSIDQHQAWRTEFGHSEPSTHSPQPIDHLNEEPSFLFSSIPPPKVKPISSESVAGTNISSRKETLDSSQLSMKASKQFKKTSQQSQITVSQRSTKSSLKFTKSKQPTPGTRKTYLPSDAIETARIKIDRVMKRRKLNDSEDERNIPLLVSQIYQAVGVQTEKDGLPRFEFDQDAFCDAQKDERLETHVLAPIFQSQYTFESTRIEVAEMHFQQFLLRVYTSCKSRMRGRSSDLVWIEKMRTFTKHQELLEKYWYRINLKFCTEPDQIVLNKFREMFPLYLFYVEMINTVVPRPAGEELSYEAELIQASAIYINYVRKIDEFLMDFSQRWLRSKAGMEEEKAEARYLRELKRRDENATAMKNHLWAFLEVWMEKQRPSLFYENCTHTGLFLYTGRAFFNDIFIYSAENFVQFIQKNSVERHVG</sequence>
</entry>
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            <taxon>Pucciniomycetes</taxon>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
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    </dbReference>
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        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
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        <source>
            <dbReference type="EMBL" id="EFP75881.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="208" mass="24154" checksum="3F1828A8A13CA39E" modified="2012-04-18" version="2">MALPWETDGGVNGRPNSIEVLLEWLSAPGNYDRWIQADLKAPLTQEIHREMIRKGIHHQTPHGISLKIYMLHEGYHHACHYRRQTPEDLRRQFPNGYLSFAGYADMICRDWARLENIFVPPDDLAPDATDPDPLARERIMFNTEAASQIVGFVDHIQAQYHETMEQIDGYVPWSTYFLNNQEDRANYPLLHGLDPELILRRYANLTMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>E3JV84</accession>
    <name>E3JV84_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    <gene>
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    <organism evidence="1 2">
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
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                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01290"/>
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    <dbReference type="InParanoid" id="E3JV84"/>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="151" mass="16969" checksum="4364004D68D00060" modified="2011-01-11" version="1">MAQHSSVPRTQTVREKLTLSRLHIIGVISPAQTLAIQHIFGHLIILDHQDCSNYISISPISTRRIQALENHSLSSPHRFSSERRAPLNLLWSVHLSPSVFCKSSSCKFSRDWVSPFDLLLLLLLLFQAVSHSSPSAFMHCFLVELDDSVVA</sequence>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP77077.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003321448.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JYF2"/>
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    <dbReference type="KEGG" id="pgr:PGTG_03033"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03033"/>
    <dbReference type="HOGENOM" id="CLU_1960672_0_0_1"/>
    <dbReference type="InParanoid" id="E3JYF2"/>
    <dbReference type="OrthoDB" id="10640686at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77077.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="128" mass="14262" checksum="1D8D12999E51155C" modified="2012-04-18" version="2">MDKAKELECNFGLVDDSQPKDDPVERARNQTEAEIKKKCKYFYELEPIMVDQPAAIPLHIHEQGDKRGTNLAAALILTRPEETEMIPLNGADKLVDARAETHSNVNLDNTDNAGLTLSQVIPQKQHSA</sequence>
</entry>
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    <name>E3JYW9_PUCGT</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
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    </protein>
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        <name evidence="3" type="ORF">PGTG_03200</name>
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    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
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    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JYW9"/>
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    <dbReference type="InParanoid" id="E3JYW9"/>
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        <location>
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            <end position="25"/>
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            <end position="684"/>
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            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP77244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="684" mass="78616" checksum="28F204ABE6B54C34" modified="2011-01-11" version="1">MIQKKNPSFFGIGFLLLACLPQGSSIFAHPLEEIFQGHDVLQAGQDNPHGLLTNHLSTEPRDSQQVPSSIEPQRSEQDPSIQKQLIVDNEWLNQSGFRHSHPGLPAGNLPDIIEAPVSDQEPNGLHNQHSAGMISSAISSPSNHQIRVEDYLEQGLVEFAANEYPSQRQHFLFQSPSIPPNSPTLTTLASESNPSWENQHFGSASSFLGGHASMDRSPKMHEEPNQYDRVPRKRQSFDHERLTPQDPKKSKKTSMKKDGSITQEPSFSIINSADNYLTPSSTLQRLERKPAIKYEKIIGANRQSWKLVDLTQTKAILDVPEHSTIMNGKPEWERVTRLDIFGLDLKPYGYVHTLKFPGERRWKKNDFLETDVLHKSGFWRSLATTNRDLPVLVQEAINPSVSPLHKTLEKSRNLLEEIFFRDGQFLLALTSKSQSKNDLQTQKQQKEEGTVDIKDELKRSQLRSHQQHQLLEWLIKLFGIGMGSPTLLFELFELDQPYDSDHQLIRFQEMLYEYLRIEEPKSEYMICNLNPISPHEQRKVSHAGALKTQVALNALGIYYKYSNRAQWEMMFESDADFVNFFGFLKIYEINRMAHRVFGYFSQWAALSFFPWELRKLDFGRMVVGGGDRDSRAIDNGLQRFRRVAQNPRWKNLEKHFVELRLVHHDQLLIQAITPATNLMFCSQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3K098</accession>
    <name>E3K098_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
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    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
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    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP77723.1"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03679"/>
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    <dbReference type="OMA" id="RYKWLVG"/>
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        <property type="entry name" value="ANL_N_sf"/>
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    <dbReference type="PANTHER" id="PTHR24096:SF295">
        <property type="entry name" value="ACETYL-COA SYNTHETASE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24096">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
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        <property type="entry name" value="AMP_BINDING"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="123"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="319"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="2">
        <location>
            <begin position="122"/>
            <end position="481"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP77723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="663" mass="73071" checksum="07651F4814066C1C" modified="2011-01-11" version="1">MTHPDQLLASTSRPVGDEIPCCPLHEFLFSNPFRQDSPIITRAPSPVTRAHHIPLIPPQKPIFMDSDPDSIEHPLTWLQLRQRSAAVGYQLNHQLGLNPSALVTSQASDHPPGRSSGLLSPVVLLYLANGIPFVTILLGALAAGLTFTTVNPSYTCHELVDVLRASKPALIVTSPFGLTTMLEALSLLQEPQLRRWLMEHIYIVDSPTTDRLPSCFFKDSKTSQTKAPNGEIATFADWKTLLQPPPLGTDYQPFQFSKPARESRLRIAAIIWSSGTSGKSKGVLLSHYAMVHSIISFWHQKLDYGPDERTIGLVPFCHVMGLQAIVLFSIAAGSTVYIMQKFEPRRYLEEITKNRITSLQIAPPIAAFLAKSPLLDDGRYDLRSVTNSMSGGAPLAPDIVKAVFKRCGFLVKSGYGLSEAGHVANQLGATISDLLPQLGTVGEVMYGVELKILSVRTKQLAARNEEGEIVIRSPSLMSAYLDNPLETSQTLDREGWLYSGDLGFLDSHGRLSITGRLKEIIMVKGYQVSPSDLEMLISKIDAVGEVAVTSFYCDDQATEFPRAYIVPQGAELTALCEIVDNSHRSPALHPRYNDLTKLALQIKEFVESHMVHYKWLRGNIILTSHIPKSPSGKVLKRILSTIQGVEIPLYTSPKESDRPSSKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3K0L1</accession>
    <name>E3K0L1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_03792</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77836.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322255.2">
        <property type="nucleotide sequence ID" value="XM_003322207.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K0L1"/>
    <dbReference type="GeneID" id="10540820"/>
    <dbReference type="KEGG" id="pgr:PGTG_03792"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03792"/>
    <dbReference type="HOGENOM" id="CLU_038374_3_0_1"/>
    <dbReference type="InParanoid" id="E3K0L1"/>
    <dbReference type="OrthoDB" id="2504287at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058913">
        <property type="entry name" value="Integrase_dom_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177:SF1">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24764">
        <property type="entry name" value="rva_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="108"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP77836.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="191" mass="22109" checksum="733D01FC9C8F85B5" modified="2012-04-18" version="2">MRSQLLSELGVNVCHRTVERYLRRLKLKQRQNDLANGKITREEVVELVRHARDALLANTAGYRRMRQILVTNYGVHLPRQVVYDILREVDPEGMQLRLKKTCKRRVFHTTGPNHIWSADGHNKLNRFGITVYGFIDAWSRKILGIFVHVTNNDPRHIGAYFLHLVRAIGGIPWKLTTDRGTETGKMGSFQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3K0S8</accession>
    <name>E3K0S8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03859</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP77903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322322.1">
        <property type="nucleotide sequence ID" value="XM_003322274.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K0S8"/>
    <dbReference type="GeneID" id="10541044"/>
    <dbReference type="KEGG" id="pgr:PGTG_03859"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03859"/>
    <dbReference type="HOGENOM" id="CLU_1611598_0_0_1"/>
    <dbReference type="InParanoid" id="E3K0S8"/>
    <dbReference type="OrthoDB" id="2495293at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171670" description="Secreted protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="165" mass="17391" checksum="33D1D535F0823E4D" modified="2011-01-11" version="1">MKLIILAVFIATVACLVAGDPLPLTKRDGFPNSAMKPMQCPSGNTEVSCLVTDSSWPTNDFPCGQDTIRNNVIKAITNGILKKTAADDVEGSPPFELGPACQGGPLYKFQHNTVTAFYVVKTECDCKAGVVPSCSNVVEKSQDACNISKIVYCGASSGQSHCAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2N8</accession>
    <name>E3K2N8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hydrophobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04563</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78607.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003322978.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2N8"/>
    <dbReference type="GeneID" id="10536459"/>
    <dbReference type="KEGG" id="pgr:PGTG_04563"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04563"/>
    <dbReference type="HOGENOM" id="CLU_1866157_0_0_1"/>
    <dbReference type="InParanoid" id="E3K2N8"/>
    <dbReference type="OrthoDB" id="2508389at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173493" description="Hydrophobin" evidence="1">
        <location>
            <begin position="26"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78607.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="137" mass="14785" checksum="CCA74DF32BF39406" modified="2012-04-18" version="2">MDTFKQILVLCFLVACELTHHSARAVGLGAAVLGKIMRPDDACNKVETSETKIHFCFKDNRKKTDGFVTIAQPVQRIAQGVTTSADCTKLGNGFQVTKCCSVGSFSLAPKAGKPLFNPNFFVNVTPASFDLACNKQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2R7</accession>
    <name>E3K2R7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04592</name>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78636.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323055.2">
        <property type="nucleotide sequence ID" value="XM_003323007.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2R7"/>
    <dbReference type="GeneID" id="10536489"/>
    <dbReference type="KEGG" id="pgr:PGTG_04592"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04592"/>
    <dbReference type="HOGENOM" id="CLU_1422053_0_0_1"/>
    <dbReference type="InParanoid" id="E3K2R7"/>
    <dbReference type="OrthoDB" id="2505938at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78636.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="191" mass="19803" checksum="4882AF2FDE3FCE38" modified="2012-04-18" version="2">MVVEGLAVTSRVTGLPGPLLDPTATTSGLSTVKNTAIPITPQQLRKENSIVYRDSSEIMPPSLPPTLTTVTLKFDKTTVLLPATKKTRVSELKTGFLDALKGTKTSPSNGSLEDSTCFQIFRASKTAANLTEGGPAGGAGGGPADSWDALADDHATMSDLALEEAETLGVGFKNSKGPSLSHPISSLDHPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2T8</accession>
    <name>E3K2T8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04613</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
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    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323076.1">
        <property type="nucleotide sequence ID" value="XM_003323028.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="10536510"/>
    <dbReference type="KEGG" id="pgr:PGTG_04613"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04613"/>
    <dbReference type="HOGENOM" id="CLU_1496960_0_0_1"/>
    <dbReference type="InParanoid" id="E3K2T8"/>
    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="180" mass="20318" checksum="22430DB95831DEC9" modified="2011-01-11" version="1">MTQCWSATIDAGRVDTTGIPTQGFLHPVFDSQPTISRPRPNCSRADRRAAQPGHLQRQIHTIRRKNWTLRINPPLVTTEASLEHIWINSYPTMDYIAQGFHPSLHITKPNYNPQATQTPSLKIPHVIAQQKGSYNHFLSLTPADKPDSCLAEAIIFFQDLIGQDDWCATEMPMSKLKFAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3F0</accession>
    <name>E3K3F0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04963</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP79007.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323426.2">
        <property type="nucleotide sequence ID" value="XM_003323378.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3F0"/>
    <dbReference type="GeneID" id="10530828"/>
    <dbReference type="KEGG" id="pgr:PGTG_04963"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04963"/>
    <dbReference type="HOGENOM" id="CLU_1321444_0_0_1"/>
    <dbReference type="InParanoid" id="E3K3F0"/>
    <dbReference type="OrthoDB" id="2496336at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79007.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="208" mass="23599" checksum="567CE8EA41B9CD55" modified="2012-04-18" version="2">MEKNIASDTRTGLDYLLKLRTTICLLTLTAGLAVCALGFKANQLLGNDRNRVSRATSGIQLESKALLNLTRTSLVMSASLIVNTLFLAVFLSLRWHHPNPSKHECFLQKSRAMIKLIMEGQFFAVCAVFSFLVTLLSNSNTKFEPTPTNLQDFRLALGIPGRYIDNHYIMALVATAWIFTFGYLFGFTLEAWTIYRFKRFQKLIFIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3K4U4</accession>
    <name>E3K4U4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_05580</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323678.1">
        <property type="nucleotide sequence ID" value="XM_003323630.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K4U4"/>
    <dbReference type="STRING" id="418459.E3K4U4"/>
    <dbReference type="GeneID" id="10531083"/>
    <dbReference type="KEGG" id="pgr:PGTG_05580"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05580"/>
    <dbReference type="HOGENOM" id="CLU_1504159_0_0_1"/>
    <dbReference type="InParanoid" id="E3K4U4"/>
    <dbReference type="OrthoDB" id="264917at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047126">
        <property type="entry name" value="RNF141-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12109">
        <property type="entry name" value="RING FINGER PROTEIN 141-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="124"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="32"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP79259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="179" mass="20125" checksum="5CCBBFBA2E51DE8D" modified="2011-01-11" version="1">MKNTSGMSSSLVDPKNADESLDGGSCFKAEDCAICSQRCTGYRDLPCGHSIHRSCAKKWLSINPTCPFCRQPVPDEGVEKCAQVTRVPRAERSPITIDAQQSMWSISHSAQRDHEIVIRRRGRVCLIMFFFLSWIILESLMSALRSNLMESSILTLSSREDLKADLQSKTTRMCLVIHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3K531</accession>
    <name>E3K531_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Copper/zinc superoxide dismutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_05667</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323765.1">
        <property type="nucleotide sequence ID" value="XM_003323717.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K531"/>
    <dbReference type="STRING" id="418459.E3K531"/>
    <dbReference type="GeneID" id="10531170"/>
    <dbReference type="KEGG" id="pgr:PGTG_05667"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05667"/>
    <dbReference type="HOGENOM" id="CLU_1455098_0_0_1"/>
    <dbReference type="InParanoid" id="E3K531"/>
    <dbReference type="OMA" id="HVHEHAV"/>
    <dbReference type="OrthoDB" id="2015551at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004784">
        <property type="term" value="F:superoxide dismutase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.200">
        <property type="entry name" value="Superoxide dismutase, copper/zinc binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036423">
        <property type="entry name" value="SOD-like_Cu/Zn_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024134">
        <property type="entry name" value="SOD_Cu/Zn_/chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001424">
        <property type="entry name" value="SOD_Cu_Zn_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10003">
        <property type="entry name" value="SUPEROXIDE DISMUTASE CU-ZN -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00080">
        <property type="entry name" value="Sod_Cu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49329">
        <property type="entry name" value="Cu,Zn superoxide dismutase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171761" evidence="1">
        <location>
            <begin position="22"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Superoxide dismutase copper/zinc binding" evidence="2">
        <location>
            <begin position="50"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP79346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="186" mass="20123" checksum="C812E4D7875563F6" modified="2011-01-11" version="1">MAHTIATYIFWSLLIVGQAFSLSVTSPNLNLKATKATQGGIAHLSGKKNVSGLFQFSSPEDSEWVNIVISMRGLLQFNLTEGFAYHVHEHPVVDGNCSSALLHYKVPTPKPSCTSEIKNYCQPAEFPTRHGNLPGLVDVFTLRINDNLIDLTGPNSIIGKSIVVHGANLTRLACGNIEPWTPGAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K5N4</accession>
    <name>E3K5N4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79787.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324206.2">
        <property type="nucleotide sequence ID" value="XM_003324158.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K5N4"/>
    <dbReference type="GeneID" id="10533312"/>
    <dbReference type="KEGG" id="pgr:PGTG_06108"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06108"/>
    <dbReference type="HOGENOM" id="CLU_029209_0_0_1"/>
    <dbReference type="InParanoid" id="E3K5N4"/>
    <dbReference type="OrthoDB" id="10306160at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="184"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79787.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="634" mass="72661" checksum="5FBE17F8DEBE11D1" modified="2012-04-18" version="2">MMVDLAYFSLTTSLWFLVLFPVGLVSIKADSSLGSLGRSIEEHSGDRAERNAGPLSAIPVKRKSRNRMPSLVPCVRIPGPGKRRTSDPAAAERRARVTDNWDLNRDQVLLLQQYKQEFPAYQEELERQLRWLERHSKKISPAELQMARKVFEGSKQQISSLIQTRLLELGKVDERATAIDDPNDPVSSSSSSSSLSDPPTKSPSLGLKLAQLAYIPAELRTQITEQSALLLWEDAKKMLTTQRKIYEAEELKSQRKPRWPSASFSTLFTSSTTTNNDHSRFDPLMPSKYAFKILDFLVQNQLITLQSSRAFFHDLPFVQTVISYSLARIKAEVGASYWKSIDHLTAHWYWPSLNKVFSALGKKEESIIDFVFHVGKLKALVHLSNGIESTDYFYSAQWAEFSKQFPWRKCFERLGEDVGHQSGTEASLSRLEHGDHQIALHDPPIISRKHSQECPAAQGTEEEEMERLIRLLVTTRHSQGITIPKFGLRLYRTETINLFEFMNNALYPGIVSKLVKNEEFERIMHDSIGLVDQKHERAESLFYLSLLYSRYHSLVIDTQSYLNLLATTLHARKSKAPHGSFAQLVEQDPVLAVYSDRFDWILEHYKKLYNSIHSRNPLLIHHLDPKFRELYLSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3K6N7</accession>
    <name>E3K6N7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05258</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EFP80033.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324452.2">
        <property type="nucleotide sequence ID" value="XM_003324404.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K6N7"/>
    <dbReference type="GeneID" id="10534050"/>
    <dbReference type="KEGG" id="pgr:PGTG_05258"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05258"/>
    <dbReference type="HOGENOM" id="CLU_2159652_0_0_1"/>
    <dbReference type="InParanoid" id="E3K6N7"/>
    <dbReference type="OrthoDB" id="10599031at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP80033.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="111" mass="12510" checksum="B85D6B9B7A826FA8" modified="2012-04-18" version="2">MKSKKLSSAKKRKLNEGSSHQTKSPEPDSKSDSVIDLGVNLLNEYLEFVKIPPGDSDGILHILTENKATNPQLFQSKNITREVMKEWGLHDVFIAQLQDNVLKFEKQTSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3K7F5</accession>
    <name>E3K7F5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_06235</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324698.1">
        <property type="nucleotide sequence ID" value="XM_003324650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K7F5"/>
    <dbReference type="GeneID" id="10539285"/>
    <dbReference type="KEGG" id="pgr:PGTG_06235"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06235"/>
    <dbReference type="HOGENOM" id="CLU_674610_0_0_1"/>
    <dbReference type="InParanoid" id="E3K7F5"/>
    <dbReference type="OrthoDB" id="10275195at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003903">
        <property type="entry name" value="UIM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02809">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00726">
        <property type="entry name" value="UIM"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="363"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="219"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="292"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP80279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="387" mass="43558" checksum="46183D957721C842" modified="2011-01-11" version="1">MPSLGPGAGLADRGSSEVLGASHHQSQQLQANHEENSEDDLARALEASREEYERHEPDIYQSLEEALVRIRSITDIHESNERNSKDVLAHVLEASKEEYEQREADIDQPLEEALVRSLMDESHGNNSEDDLARALKASKEEHEQREPDIDQLLEEALARSLMDESQSSSRESDAFLLDALSDAELEKFQESTRGNHCGLGIEEQNLARVLEMSKKEALDRELENPLSQALQFSPKDRNQASTSHDASGPNKTLHVEFLGSPEPSDTERPVVSRVLRSPMSAKEYLGRQQNLMDEPLTRHTSPSSVLANKSPGSEFQENYHPNGYSGQPDNPDWRPRYAERSTSQGGPGNYIQKTSFKKKSVDFLLSVPKFVWVLVIAHLLWYYLSKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>E3K7M1</accession>
    <name>E3K7M1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">E2 ubiquitin-conjugating enzyme</fullName>
            <ecNumber evidence="1">2.3.2.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_06275</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="7">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="1"/>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80319.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324738.2">
        <property type="nucleotide sequence ID" value="XM_003324690.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K7M1"/>
    <dbReference type="STRING" id="418459.E3K7M1"/>
    <dbReference type="GeneID" id="10539327"/>
    <dbReference type="KEGG" id="pgr:PGTG_06275"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06275"/>
    <dbReference type="eggNOG" id="KOG0417">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_2_3_1"/>
    <dbReference type="InParanoid" id="E3K7M1"/>
    <dbReference type="OrthoDB" id="7851174at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000060">
        <property type="entry name" value="Ubiquitin conjugating enzyme (UbcB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24068">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="9">
        <location>
            <begin position="8"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="157"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="201"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="218"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="235"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP80319.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="271" mass="30096" checksum="9EE0366E937BF2FB" modified="2012-04-18" version="2">MPPTIPPQTLKRINRELLTIQSELPEGILSVGPKSLANIYDWQGSIAGPSGSCYEHGVFHFNILLPHDYPFHPPQVSFKTRIFHPNINPQGGICLDILTHKWSPALSIQKVLLSIICLLTDPNPQDPLARDIARLYISNRREFEATAKRWVDMYARPPPPAISEKVTEEPKKAPLKSTTQKSKPAIIDIDDDEIEVVKPGQTTQSLQTATTSRKRSGEYSNTLQTATTSRKRSGDYNNNATSLSSGGPSTPVESESNPQAKRPRKQVIELD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3K846</accession>
    <name>E3K846_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06198</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80242.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324661.2">
        <property type="nucleotide sequence ID" value="XM_003324613.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K846"/>
    <dbReference type="GeneID" id="10534584"/>
    <dbReference type="KEGG" id="pgr:PGTG_06198"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06198"/>
    <dbReference type="eggNOG" id="ENOG502QUY7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038374_5_0_1"/>
    <dbReference type="InParanoid" id="E3K846"/>
    <dbReference type="OrthoDB" id="2505297at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058913">
        <property type="entry name" value="Integrase_dom_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177:SF1">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24764">
        <property type="entry name" value="rva_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase core" evidence="1">
        <location>
            <begin position="31"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP80242.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="179" mass="20647" checksum="455366E654749434" modified="2012-04-18" version="2">MACTSTLAMINRTLDPDGVQNRAKRVLKRRVFKTPGPNFIWSADGHDKLKKFGITIYGFIDAWSRKILGLFVHVTNNDPRHIGYYYLQLVKREGGIPRRTTTDKGTETIQMAGHQINLTEEFNLECADPSQSHLFTKSAHNQKIECLWSQMMKQYNCQLINHLFEAIEKDCYDPNDPVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3K9C3</accession>
    <name>E3K9C3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Dyp-type peroxidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_07310</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the DyP-type peroxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP81058.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325477.2">
        <property type="nucleotide sequence ID" value="XM_003325429.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K9C3"/>
    <dbReference type="STRING" id="418459.E3K9C3"/>
    <dbReference type="GeneID" id="10536378"/>
    <dbReference type="KEGG" id="pgr:PGTG_07310"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07310"/>
    <dbReference type="eggNOG" id="ENOG502RUI2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015125_2_0_1"/>
    <dbReference type="InParanoid" id="E3K9C3"/>
    <dbReference type="OrthoDB" id="3207336at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049509">
        <property type="entry name" value="DyP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048328">
        <property type="entry name" value="Dyp_perox_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006314">
        <property type="entry name" value="Dyp_peroxidase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01413">
        <property type="entry name" value="Dyp_perox_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30521:SF4">
        <property type="entry name" value="DEFERROCHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30521">
        <property type="entry name" value="DEFERROCHELATASE/PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21105">
        <property type="entry name" value="DyP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20628">
        <property type="entry name" value="Dyp_perox_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51404">
        <property type="entry name" value="DYP_PEROXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0349">Heme</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0575">Peroxidase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="DyP dimeric alpha+beta barrel" evidence="11">
        <location>
            <begin position="99"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="domain" description="Dyp-type peroxidase C-terminal" evidence="10">
        <location>
            <begin position="385"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="526"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="526"/>
            <end position="537"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF20628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF21105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP81058.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="579" mass="64509" checksum="F6C4AF8AE9CB38CA" modified="2012-04-18" version="2">MISIRMTDCPSSFTCYILSNVSIAIVVECLTSDLLQRPKFDEQSLLKDINSKTASFARKPYLKGTDIIFPFTFGKYLDLIRQFLQLNQAEYDLMVDLKNVQGDVIIGLQKRQEAFWFGTLRSDPSSIEGFRKNLKTHLLPLITTTQGVIDAQKVIDDHQRKQKKGEAKQWLPITHVNLGFAYNGLKKLGLKPEEIPTGRDGVFSKGQKLDAVKNLGDPMIQATKKLNTWSRDFLGEVNTIDFVVLITAPDSNLLNQKLHQVRRMFNGFLSHSFLRQGNVRPGDQYANEHFGFPDAISMAKIKGVNARAEDRKAGIVEPGVILLGYPSANGNSTAPDANQAWLKDGSFMAFRELRQLVPEFHHFCDETAKNMKNLNVSGDFIGARIVGRWKSGAPLTLAPKHDNPALSTAQDFDYSDELKQERCPYAAHIRKSNPRNGIQGADPQKTTLPHLMIRNSIPYGPELTGEEKYFKRTIENRGLLFVAYQSDIVAGFQFVQKAWCNNPSFPPQTALNVSAGLDLLIGQHSDGSPRTAQNITPSVPGGNADPRNTVTAFQPFVVPLGGEYFLMPSIKAINEKLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3K9J5</accession>
    <name>E3K9J5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07355</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP81103.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325522.2">
        <property type="nucleotide sequence ID" value="XM_003325474.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K9J5"/>
    <dbReference type="GeneID" id="10536425"/>
    <dbReference type="KEGG" id="pgr:PGTG_07355"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07355"/>
    <dbReference type="HOGENOM" id="CLU_012972_6_0_1"/>
    <dbReference type="InParanoid" id="E3K9J5"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="367"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="405"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81103.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="476" mass="53586" checksum="7285745C10720F4A" modified="2012-04-18" version="2">MAFSQNQDLEGALPTTNPPSTGIFSGNQNGQNMNIDPPPPPMSHNRIRHNRTYGINRHTQSANTSTGNRPGPGGNLGNGTYGIPAENEEPQEMDEPRPRPAVIIKEADLKYDGENFEDFLDRFELAAEIYGAGGFDKARQVCRFVKSKELKKELESMDGYDTRNWTILRASMMELWGGRSLDQKKGDGKKREKMVEENPPEMLQKQVDNLAKAIETLTGKLENQPTTEYSRGNERTTGRLYDPRPCAYCHREGHSTYTCYEAQKDEKEGIVKRDGRDFCLPNGDKIPWNPSRPIRTVVATNAAKPKPQINSVFRINYPASSEIRRETQEEIECITSLHKIEWEPPRVGSSSSKKIKLESNAATTRAEALRGRRKPPAEKDNSAMDVDQTDELEEIIQDIPATRKTPGQISDPQGKSTAQRGDKTVESELLSELDNLRIPTTFSQLTSISPTYAQEVIKKLQKRLPEHQNSKLTYVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAB8</accession>
    <name>E3KAB8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06896</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81275.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325694.2">
        <property type="nucleotide sequence ID" value="XM_003325646.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KAB8"/>
    <dbReference type="GeneID" id="10536762"/>
    <dbReference type="KEGG" id="pgr:PGTG_06896"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06896"/>
    <dbReference type="HOGENOM" id="CLU_2929138_0_0_1"/>
    <dbReference type="InParanoid" id="E3KAB8"/>
    <dbReference type="OrthoDB" id="2509876at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172501" evidence="1">
        <location>
            <begin position="23"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81275.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="61" mass="6850" checksum="B0D59B8DD6C59530" modified="2012-04-18" version="2" fragment="single">MLLKWHSIILSLYCFQLACVTAFIPPQLPGKCPTCKSDYVSMQIKDIGTCNAIFTCTHARQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAR1</accession>
    <name>E3KAR1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06801</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81180.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325599.2">
        <property type="nucleotide sequence ID" value="XM_003325551.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KAR1"/>
    <dbReference type="GeneID" id="10536593"/>
    <dbReference type="KEGG" id="pgr:PGTG_06801"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06801"/>
    <dbReference type="HOGENOM" id="CLU_046890_0_0_1"/>
    <dbReference type="InParanoid" id="E3KAR1"/>
    <dbReference type="OrthoDB" id="2505020at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81180.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="558" mass="63030" checksum="F567C067CC7574D0" modified="2012-04-18" version="2">MGSPWDHQMRGMDFNAKQEARMIRILCKTLNQQHSEACVEKVDFDLLAVFLMQEMHWNIHIRQLVDMVLDQFGPKAHPLGEPLPEPTDNHKKPIAPRTPLQSQIHHAALHVNQTINQHTQELQTNPNAPQPFCLRYPTYEKFVEYSISMEKKCKLLPKDKMVVLLDEAGRCVGVAVRPDHTKEDGIYLSPPDRALAGLNDAIKNCNWNEAKDKILYSEIRDPKPKSTPEELPSQNSATPAQQSKLPHQPPNRKPKAALPPEASETPAPKRKFNPDPPNPKPTSGKAKKKCKTSKDVEDNNNTNHHPNPLPPPKPQLNTKGKNTISYQPYGYGLGDKKSTGMQDLKIQFTKSAPPQIEESLLQGRGIGWRDEPSVEPNLPDPQKESSNCSECLEMVNMRNELRYQFALSEWINKAFLPQSVEVAEKAVEYLLANGSGLVRETLRCESNKILASRTISLNTQVTTHRDKKNALLFDADFFFGNHLGGELLLPSLGVACHGLHGYSFHGPLRILFHGVAKFYFPPDLKEKPRRYSVAFWSRESSFSAVGRYTAYKDKEKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBG4</accession>
    <name>E3KBG4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ino eighty subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81676.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326095.2">
        <property type="nucleotide sequence ID" value="XM_003326047.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KBG4"/>
    <dbReference type="STRING" id="418459.E3KBG4"/>
    <dbReference type="GeneID" id="10533221"/>
    <dbReference type="KEGG" id="pgr:PGTG_07925"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07925"/>
    <dbReference type="eggNOG" id="ENOG502QVDM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_450655_0_0_1"/>
    <dbReference type="InParanoid" id="E3KBG4"/>
    <dbReference type="OrthoDB" id="5413003at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031011">
        <property type="term" value="C:Ino80 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038014">
        <property type="entry name" value="Ies1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37287">
        <property type="entry name" value="INO EIGHTY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37287:SF1">
        <property type="entry name" value="INO EIGHTY SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="392"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="550"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="421"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="563"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81676.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="644" mass="71767" checksum="B243F995C1AB8194" modified="2012-04-18" version="2">MAPPKKNRANQPTNQATPSTSKDNPGWALKKLDGEMFTRADIQYDLMANIFNDRTFQFTSPLDQRAISFKELYVETLSRFSKAAKSTVRQLDDNPQWATNFCIASFLINIGRINTTVACKSSITLYPELRAYMRTYHPIPCLQKDEYCQTNLQDAPRIKTMLKSCQLPSEFGNEPESLPDIAKRSALGYRPPTTVVNLIFELFSVEPYVSMKYFPEGFVLHDLFSPTTIPTKLRAYAFLWLMHHFLEDPSALTDFQHESKVEAFQLARLEDIQPDSTLGDSPENIDTEEEIQWGKDMQTYRREFLKKWRGEEGIGFVDDLGTGFIAPLIEDCSSHLTSLFLFQAIVDLIFVTRFLLTECDVIRFSKSAQLTKKQKIMVQNYAQSSAIEGDEGQTSQAQLAAKGSTPGSSIPPSALIGSAKFENRKTTTKEPEKKSKTVSKARMTHIERLQSALDAIVKYGDDSDLDGPDDMLTATQIAWRRYQTDVTEDFDVAYDSDDLIRQPNPTWKPKELKHRLLGFAFPVPTANTITPTEEEMKDKVIRYVGVKRRTPASNVPAGGTTGGSSKKGSSATPGVGGNEAGLAATGAPPNPASNPPKLKRKRKANQPPPDPASITCLPKPDPDQPAQLNTQGELPIHMLSRPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>E3KC93</accession>
    <name>E3KC93_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Iron-sulfur clusters transporter ATM1, mitochondrial</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Iron-sulfur clusters transporter atm1, mitochondrial</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PGTG_08100</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Performs an essential function in the generation of cytoplasmic iron-sulfur proteins by mediating the ATP-dependent export of Fe/S cluster precursors synthesized by NFS1 and other mitochondrial proteins. Hydrolyzes ATP. Binds glutathione and may function by transporting a glutathione-conjugated iron-sulfur compound.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the ABC transporter superfamily. ABCB family. Heavy Metal importer (TC 3.A.1.210) subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EFP81851.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326270.2">
        <property type="nucleotide sequence ID" value="XM_003326222.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KC93"/>
    <dbReference type="FunCoup" id="E3KC93">
        <property type="interactions" value="355"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KC93"/>
    <dbReference type="GeneID" id="10533888"/>
    <dbReference type="KEGG" id="pgr:PGTG_08100"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08100"/>
    <dbReference type="eggNOG" id="KOG0057">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000604_84_1_1"/>
    <dbReference type="InParanoid" id="E3KC93"/>
    <dbReference type="OrthoDB" id="6500128at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140359">
        <property type="term" value="F:ABC-type transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140466">
        <property type="term" value="P:iron-sulfur cluster export from the mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18582">
        <property type="entry name" value="ABC_6TM_ATM1_ABCB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1560.10:FF:000004">
        <property type="entry name" value="ATP-binding cassette sub-family B member 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000287">
        <property type="entry name" value="Multidrug ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1560.10">
        <property type="entry name" value="ABC transporter type 1, transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011527">
        <property type="entry name" value="ABC1_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036640">
        <property type="entry name" value="ABC1_TM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039421">
        <property type="entry name" value="Type_1_exporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221">
        <property type="entry name" value="ATP-BINDING CASSETTE SUB-FAMILY B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24221:SF402">
        <property type="entry name" value="IRON-SULFUR CLUSTERS TRANSPORTER ABCB7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00664">
        <property type="entry name" value="ABC_membrane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF90123">
        <property type="entry name" value="ABC transporter transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50929">
        <property type="entry name" value="ABC_TM1F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="9" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="138"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="177"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="253"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="286"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="367"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="20">
        <location>
            <begin position="139"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="19">
        <location>
            <begin position="464"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="80"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="701"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="91"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="701"/>
            <end position="713"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00039906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00040792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00045666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="EFP81851.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="720" mass="79917" checksum="74EE31B49B23C21A" modified="2012-04-18" version="2">MLRTSNPAIIRHLPRIGCQCGIPNLSSPNRFSSHSNASSYKTSASSSGSIRKFEGNGNCRVFNSITNIIHQRTPFLSIGTRRKFSDKNDRGSTSINPASPKATNPNSSTPTHKEDWKIIRHLLPNIWPKDDRTTKIRVVTALVLLAGGKILNVQVPFMFKYIIDTLSIPLDPNTVQGAWTVVGTVIAGYGLARISASVFSELRSAIFTNVAQSAIRRVARSVFIHLLNVDVGFHLRNQTGGLTRAIDRGTKGVSFMLSSIVFHVVPTALEISIVCGVLTYNFGANYAGIALATMLAYTWFTIRTTAWRLQFRKRANEADNRAASVLIDSLSNYETVKHFNNEAYEIEQYDRALEGYEKATIGIYKSLAYLNIGQSVIFSLSLTAMMYMAAQGVLNGLMTVGDLVMINQLVFQLSLPLNFLGSVYRDLRQSLIDMQTLFNLQQTGLIIKDKPDAKPLQITQGGEIRFENVSFQYSSSGAIFKDLSFVIPAGKKVALVGPSGCGKSTLFKLCFRFYDIDQGRILIDSQDIRDVSLASLRSHIGIVPQDSSLFNSDVMHNIRYGRLGARDEEVQKVARMAKLDRSIGKWPSGWDTLVGERGLMISGGERQRLAIARSMLKDPSILFFDEATSALDVYTERELIRNIGENLLNKHRTSVFIAHRLKTIADADLIIVLNDGKVVEQGNHRELMNIENGTYRGMWLSQSATPPENSKVNVDNKIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCJ6</accession>
    <name>E3KCJ6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_08193</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EFP81944.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326363.2">
        <property type="nucleotide sequence ID" value="XM_003326315.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCJ6"/>
    <dbReference type="GeneID" id="10534144"/>
    <dbReference type="KEGG" id="pgr:PGTG_08193"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08193"/>
    <dbReference type="eggNOG" id="KOG0017">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2559392_0_0_1"/>
    <dbReference type="InParanoid" id="E3KCJ6"/>
    <dbReference type="OrthoDB" id="2506496at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48472">
        <property type="entry name" value="TC1-LIKE TRANSPOSASE DDE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48472:SF1">
        <property type="entry name" value="TC1-LIKE TRANSPOSASE DDE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP81944.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="82" mass="9867" checksum="47515C9A54692939" modified="2012-04-18" version="2">MPRRYINPYVKLLAVDSIYRWAEVFERTGCVIRDPDEYQPFGPNLKIPEDVQDSLQEHLQENPTAYLDEIQEWLYEQHEILT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>E3KD15</accession>
    <name>E3KD15_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_07472</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326494.1">
        <property type="nucleotide sequence ID" value="XM_003326446.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KD15"/>
    <dbReference type="GeneID" id="10534606"/>
    <dbReference type="KEGG" id="pgr:PGTG_07472"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07472"/>
    <dbReference type="HOGENOM" id="CLU_2062632_0_0_1"/>
    <dbReference type="InParanoid" id="E3KD15"/>
    <dbReference type="OrthoDB" id="10330654at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP82075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="119" mass="13527" checksum="1558DB241B00EDB5" modified="2011-01-11" version="1">MESQEKQLACRSPDHKAESLHGYSHLDVPNQIHPIGVCPSSIFPPPPHQVYPYLRLGLSRFGTIIRKRWSSAVLHRKIFRHTIPQLTTSLGGHESSNIQLTLDPLLNWTLHLQLLVQVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDG3</accession>
    <name>E3KDG3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_08355</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82399.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326818.2">
        <property type="nucleotide sequence ID" value="XM_003326770.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDG3"/>
    <dbReference type="GeneID" id="10529777"/>
    <dbReference type="KEGG" id="pgr:PGTG_08355"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08355"/>
    <dbReference type="eggNOG" id="KOG2533">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_0_3_1"/>
    <dbReference type="InParanoid" id="E3KDG3"/>
    <dbReference type="OrthoDB" id="9971669at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000013">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF85">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G00710)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="142"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP82399.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="242" mass="26728" checksum="0C1E76788A7C81A9" modified="2012-04-18" version="2">MTEEKFSWYQVACAFKSPHVLLAAASLFMIGSNLYGLAYFEPSNKFGYSARMTQLVSVPPFAVGFVAMLVSSYLSDHYGARGATAIAMASLAFAGYLMFMISNSTVVRYFSLFLSTAGIYSASPTLYAWIANNSAPHYRKATAIALGPIAANTGGIVSTWLFSSSQNTHYQAAPIVNICFSAGIAILCSINLIWLRSCNQKREAQKEETDVLTEKGQSIVKNMDYEEWLRLGDKYQTFRYAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDV1</accession>
    <name>E3KDV1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="3">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_08291</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPL family. K-HECT subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="3"/>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326754.1">
        <property type="nucleotide sequence ID" value="XM_003326706.2"/>
    </dbReference>
    <dbReference type="FunCoup" id="E3KDV1">
        <property type="interactions" value="647"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KDV1"/>
    <dbReference type="GeneID" id="10529702"/>
    <dbReference type="KEGG" id="pgr:PGTG_08291"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08291"/>
    <dbReference type="eggNOG" id="KOG0168">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0170">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000366_0_1_1"/>
    <dbReference type="InParanoid" id="E3KDV1"/>
    <dbReference type="OMA" id="AEPLSQF"/>
    <dbReference type="OrthoDB" id="2537092at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016607">
        <property type="term" value="C:nuclear speck"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045322">
        <property type="entry name" value="HECTD1/TRIP12-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057948">
        <property type="entry name" value="TPR_TRIP12_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE HECTD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45670">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRIP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25579">
        <property type="entry name" value="TPR_TRIP12_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 6" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="8">
        <location>
            <begin position="1575"/>
            <end position="1955"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="858"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1159"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="41"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="63"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="89"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="169"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="225"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="859"/>
            <end position="890"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="907"/>
            <end position="924"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1176"/>
            <end position="1195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1231"/>
            <end position="1244"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="6">
        <location>
            <position position="1922"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP82335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1955" mass="211099" checksum="6AC773CE519C5F2E" modified="2011-01-11" version="1">MSSYTRASGKRRARSASPSPAFPEPTPKPSNKRLRGAKPDTQSSSTTTTPSSSSYNLRHRHPSQASRVVSSSTATASVDRSNKAESRLRPSSSPKSSTTMPRKHRSSKTVISAGNLKEKANSSSHTAPLASTSNPTSAPATAKSSEQDVEMNVQHSISSDRPGNDGTDHTSALSTSDHGANDESQDEQDSPSNSEADDPDLTEEPGGAHDDFGDEMDPEHASLHDEDDDDDMDESEMAALRASEALFGFDRASTTARGIGGGSGGALAGLDAMGISGLRGLGGIMAGFTHRLKNILQSLKARGSGSSTSRLAALQELAEILAVSTEDTLAGYFQTDSFARELVGILRGESGHNESEDEEDEVALVAALTATGSSSGSYHHPSSGTEVEQMLLASRCLANLMEALPGSAHTVVHHGAVPVLCAKLLEINFIDLAEQSLSTLEKISEELPSSIVREGGLTALLQYLDFFSTNVQRTAVTAAANCCSSISSDSFEMVRDVFPILRNILSYSDQRVSEQAVLAITRVTDSYRHHPDKLQQLLTPDVLSSLTALLSPVGGTKISDNIFSAILKSFTNIGRSSPEVATNLLDAGLADTVYGILVGQTPPEIPDEHDLALELTSNSSVITQALMRKDRNQIQQAIDLVVEVLPPLPKSGIFDPKLAKKPINPQENVAMKDEEAYSTSVPEPSALPNEAINSFPGVRSAMDEDVKPDISARTLAALAYSSSSQVKESNKEAAIAHRIELLQSTSREQVVRRFNALLLPTLLEVYGASVSFTIRLKALVAVLKIAYFSKAEYLTRTLRPIPLASFLASILANRDQNSLVMYALQMVDLLLAKLPDDYHFIFRREGVMHEVNRLAESAPSGSHSSIKTRISSATAGGSRSESHISTTSNPLDEDLLQVPSSLGRHGSSSIHPFSTASTSSSSTSTKDQLIYRARHLKAKCVMAETTASTKAQDILDNIRDVVDALGTVPTPEEAKSALLKLAALFSRENDPVTSFELLESGLVEGLLRFATESRSFGPSLSVRSEILSETFFSPSEGTPAFVPLVKRLQESLGRLEGFEVLTAVASTPDDSRRSTASMLARQLKIRLVAAEGTDVPKNCANVVVSIHAIATFQAFNDYLRPRIIKAQDDERLGRPSGSSSSRLSGMLAAFAAVAGLPASSSSSPSSHLEESGFTLPSRAEAAESSQSSSLPAVAPEVPLSTHAPRRSSRLSGRGLTAEDLNREAADAPMSKPSSSAILSTSAPTDSMEVDTPRRDGMEVDDEQPTEGSPIDQEMSSRPSNVDEPPVNVQPAQDGSKMEAHTPHGTRIATPLGSRPASALRKHSNSSLAGLVASGAASRSSKPSYAAAVKAEPKDWHLAFSLGGQPIPLDTTVYGAVYAHETQPGLQSRNMWTNVYTVTFRKVDGPVPASNEDASSVDPESREEGLLPASILEGSQQASILRLLRALHGLNLDWHELKNSAEQAASSLAFAESVFINNKLTAKLNRQLEEPMIVASACLPDWACELPQQFPFLFPFETRFSFLQSTSFGYARLIQKWVGQARSDSSRRDENLGLLGRLQRQKVRISRQKILESLIKVFELYSSCRAMLEVEYFDEVGTGLGPTLEFFSLASKAFAERQYQMWRDHESDSQALHVFSRTGLFPSPMDDRTAESEKGIERLRKFKVLGQFMAKALMDSRIVDISLSRSFARLVLDYPLPLTIASVGLVDKSLAASLEHLNKYVIAKHAIEADSSLSESETHAAIQQIRIDDATVDDLALEFILPGYDVEMKPDSSATLVTIDNIEEFIELVIYWTLSKGVTRQIEMFKAGFSMVFPIRDLKSFTPDEIVNIFGNAESEDWSPEALTSAMRADHGYNMDSAAIRGLINIMSTYDVPSRRQFLQFITGAPKLPIGGFRGLHPALTVVRKAAEPGHSPDEYLPSVMTCVNYLKLPEYSSQDVAAEKLTIAIKEGADSFHLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KE57</accession>
    <name>E3KE57_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08795</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82599.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327018.2">
        <property type="nucleotide sequence ID" value="XM_003326970.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KE57"/>
    <dbReference type="GeneID" id="10529982"/>
    <dbReference type="KEGG" id="pgr:PGTG_08795"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08795"/>
    <dbReference type="HOGENOM" id="CLU_136935_0_0_1"/>
    <dbReference type="InParanoid" id="E3KE57"/>
    <dbReference type="OrthoDB" id="10281160at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82599.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="158" mass="17582" checksum="94078D7441053BDF" modified="2012-04-18" version="2">MAQSLGPEAIQALGEGSNLAQGQSFFKNYSDILTSPTPPTCIFVELIDKHRRASKNELYRQVIRRESEIALFVDTKSKLATLREIVQRDLGYPVIVKHPRLAYLRNNTVGAPSKNLGTPGTPLPLAQGQLPRPESRVLLILIGLSYAFLFCLLLSYLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KFN6</accession>
    <name>E3KFN6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09053</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EFP83100.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327519.2">
        <property type="nucleotide sequence ID" value="XM_003327471.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KFN6"/>
    <dbReference type="GeneID" id="10544812"/>
    <dbReference type="KEGG" id="pgr:PGTG_09053"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09053"/>
    <dbReference type="HOGENOM" id="CLU_1390848_0_0_1"/>
    <dbReference type="InParanoid" id="E3KFN6"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="174"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83100.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="196" mass="21967" checksum="6F5570CD2A9DD404" modified="2012-04-18" version="2">MDGSTGGSSRSVMNMQYYCSYRIPNGAHRVVEQDTGTRGGDATHKCERSDGQLRNPRGYATEPQTRYENIFVEVAPVPRQCDVRRPNMLFSWDCDIGRTLIELIPNQQHSFILRMLTGSHLIEALKAHAYISRSNCVCEVVSWGVVKSKMSKHGWLHMLRRPERPSPGLRHSRAAASKVASAVEVSKTSTRPSSTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHL2</accession>
    <name>E3KHL2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09500</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328206.1">
        <property type="nucleotide sequence ID" value="XM_003328158.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHL2"/>
    <dbReference type="GeneID" id="10532474"/>
    <dbReference type="KEGG" id="pgr:PGTG_09500"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09500"/>
    <dbReference type="HOGENOM" id="CLU_039964_2_0_1"/>
    <dbReference type="InParanoid" id="E3KHL2"/>
    <dbReference type="OrthoDB" id="10381321at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="370"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="414"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="507" mass="56300" checksum="C53C8CE5536AA8CC" modified="2011-01-11" version="1">MTRRARLWPSRRWFIAQAWDPRPNTLDPANWSTNPPVFHSTTAFSSEMAFPNSIVDYSGQFIDYSGQGNFLNHSVLQPIHVEEPVISTTTESLHTSLAKSLPKPGDLVKMKLKEIVIKTRLRLFLASKNKRQERVWLTLNSMDDFPVQIKASGLSFVDFRRDIIRACNTEIANSATIIQAALDSQKYDITWTGSIDRVAGWKKADKIKIDTPLVFDAWMNAIRTTKKPEVALLIKMVNPNTSIQQGKKVDLLAKRAAQQAAIDKDRALSLKRKSTGDDDSEGGSPPPDGDLEPEDWDNINFHMKRIYDKYPIKSDYDPKIPVFVNAGETDKYILLTNKACQEWGIALAGIADGVDMDTPPRSLPYESLQANRRKKARVVPPVGGHAPGQAPGQMPSMMEIIALADALNARRGKGDIEAIPSSPGHSHDPDEDPPMADYLAFLGKKLVNTDAVLEILISNEMMSHKSFAGGLKRSDVCGLGLSMGAVTCLFDNVARYDRYLAAKKIRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHR5</accession>
    <name>E3KHR5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_09553</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EFP83840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328259.1">
        <property type="nucleotide sequence ID" value="XM_003328211.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHR5"/>
    <dbReference type="GeneID" id="10532522"/>
    <dbReference type="KEGG" id="pgr:PGTG_09553"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09553"/>
    <dbReference type="HOGENOM" id="CLU_030979_1_0_1"/>
    <dbReference type="InParanoid" id="E3KHR5"/>
    <dbReference type="OMA" id="PLCHGIQ"/>
    <dbReference type="OrthoDB" id="2513234at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="346"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="291"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="428"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="461"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="491"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP83840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="511" mass="55896" checksum="6101ACC5CA377FF5" modified="2011-01-11" version="1">MAPLISRSPVGTRSTTSNVTTRPATSTAAGKRVARSPSPPGDDGVGDSNGNQGSSGGGANRGGTPPPLRDPAPHFFRDAPHPPHPADDPRTRAMIINAASNQFRDEYKLREDGSNFRDWLREIRELALLCFNREDWYDADRSHDRFDILAKSALLISVCRPIKADLYDVGSSHAIMQQLRRRFTTFSRAAQLNKWIDLFKVPCDKNSEPSSIATLYRHRLADLRDAGVCITADSILSIILHCSIAHGTKLRHEFDMAIDRELSVDQDDPLSFNNHIDILTACREKVRARESDRHRNPLPSGFSAGFHQSQPPARSASVESHPDNVYVLAGRPANFKSPAPGTTRTCFRCGATGHLISNCTAPPTFPAAHRDFHGVPPPPPPPGLMGPQYQAYYPILAPPFPPFPPFLPPPPPPAPAPLRPADSYRPVYKQPSGLGNRPAAREAEAGSEEPAPAGHVFDTNPDMQPSFSDLSFTGTDTRPETLFDTGATHHLTGDSERDPDFSWAQLNSYPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIB4</accession>
    <name>E3KIB4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_09752</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EFP84039.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328458.2">
        <property type="nucleotide sequence ID" value="XM_003328410.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIB4"/>
    <dbReference type="GeneID" id="10532734"/>
    <dbReference type="KEGG" id="pgr:PGTG_09752"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09752"/>
    <dbReference type="HOGENOM" id="CLU_018552_2_2_1"/>
    <dbReference type="InParanoid" id="E3KIB4"/>
    <dbReference type="OrthoDB" id="2505664at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF85">
        <property type="entry name" value="GH03217P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="8">
        <location>
            <begin position="197"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP84039.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="371" mass="42071" checksum="A0DF3205E087432A" modified="2012-04-18" version="2">MPRSSQRAESIRNMRRSLCLHVTQAAFEARYKDTDDESSASEDSEIEGLVMAIISIKKRRYLAERVRLERAPDITEYLFRLDTGRFKQEFRMSQKSFHQLLSLIKNNSVFHNNSNVPQRPVRDQLMVTLRQMGMSGNGSSIGILACFFRISEGSVILYCSRVVEAILALESTYVVWPNHEGREAIASDIANSTGFERIIYYLTGWPGCSHDTRLWENCDLNQQKARFFSPGQYLIGDSGFPAESNLVPAFKKPPHGPMPRLKKKFNQHLASLQVCNEHCIGILKGRFQSLRGIRMELTSVETMKRITQWVSACVVLHNFLLSDESPAIDHDLIDPTAAIDDDILPRRGANSPGNQLREQVYQGVLDYLGVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIW7</accession>
    <name>E3KIW7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_10620</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Dynamin-Rho_GEF"/>
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        <property type="entry name" value="NUCLEAR FUSION PROTEIN FUS2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22834:SF20">
        <property type="entry name" value="SH3 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="DBL homology domain (DH-domain)"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DH" evidence="2">
        <location>
            <begin position="154"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="50"/>
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        </location>
    </feature>
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        <location>
            <begin position="268"/>
            <end position="298"/>
        </location>
    </feature>
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        <location>
            <begin position="540"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
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            <begin position="282"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="576"/>
            <end position="624"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP84242.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="690" mass="75992" checksum="3DE709EB535870B4" modified="2012-04-18" version="2">MVNKPPTRRTFNNNNNNRMNTPTTTTRKRLLDQTQDQSILWVDRIQNHSNNLTTTNKPSPSPSSPSTNSSNSSFHSTSSQPNQQQQQQQYPPSPSASSSTSTSSYSPSSSAFPSSASSSFTSPANTTRQTILSTNSIINTNNNNNNNNNNNTFKRTRAIEELVETEAIYASDLVIIRDIYLYRASGPPPSPPTLSTNDRQIIFSNITQLTQLALSLSEALTQANTQNQIGSCFIEFLPKIEKLFTVYCSKYGPASKRLEEFTRKLDRKQKKLDKSSSSNSGHHNHNHHHHHHHLHKPREEQLGIKYLNDCKKLCEGRTSAWDLGSLLIKPVQRCLKYSLLLDQIIKYTESDDPDLSSLIKARDGMVRVADSINETKRRHEVVGEMISRSGPLGTRYGVRLGSGSSNNSRPTGGKCWPSPPGLAELIDQVRRSAKALDQLPLVLIGSQNSIVDWIVAADRLVDAFKHTFALSSSPSAPPPPGILLSDLQAYQHLVLRGSLNGPLKFLRERIKKEIIPRIEELKGLYTKPLMLIEKYEKRRQMIGSPSHHPASPTGSTSRTRRTSEGGGGSGTDQPIFPLSSASSFSSHPLLPSSHTTTPISIPSSSSSSSTAATSTTTAATSTTTTTAATLATEDGMDYQLKEIQRFLLDQLPKLIHLSNSALNRILANLFRSAQLCHSDLIVLINKALNI</sequence>
</entry>
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    <name>E3KJG4_PUCGT</name>
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            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
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        <lineage>
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            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178290">
        <property type="protein sequence ID" value="EFP84439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003328810.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="10545949"/>
    <dbReference type="KEGG" id="pgr:PGTG_10159"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10159"/>
    <dbReference type="HOGENOM" id="CLU_011079_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJG4"/>
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    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173448" evidence="2">
        <location>
            <begin position="27"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP84439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="623" mass="71693" checksum="73A306FAEF32387A" modified="2011-01-11" version="1">MQHMSRRVQFFCVVWYAIGAVQVVLSSLNAQAHTGAYVNPFAEVDTVESGIESGAHSLIDSTNLRNEHGNIPQGYEERKTEIQSIASDRNELKREIDQLIPVGKFVTVRSELKKLHGAFRKPSQERVRQLGGLELHLDPHSFTSNADSFEPATTHASNAYYFPQAGSQGNSFTNLNIGQTQVRRRKAKISNLFNPTPTSRGTSSRLPPVLEYTDHELTPLTSSSDEQQFLAHKPSDALELNKSSSSSSTPTVLEAFLKEKNIPIGDLLKTKAGEDLPASEKIKTLNALHNLLLEFKSSDRLPNPAEAEFQLNFLRSFFLLGDYIMRYELLPSEVIGTVEIFKSKNLVEMVEWHTQLLFHKLGRDFFESPESVVPELEFLTSGRAVKHFHRSIKALPDEGQKQVVHAVLGTILSRWPNQFLSDQEVSPSARFSQIRQSFFDAKLLEGAGSHSSAITRLPDTKNHIEQDDRLRLIRLTEDLVTFFEGPEMTKALNQRIEYQLVWYMLDFISHYHLPILQEVVRKRDQNWLPRQFIFMTSHFRKYRNRNVDNTPGAKEYDMGPFKEILSHQIKKNRTFIKWINTYIFKIFPYTGWTYPHESDKYLRLNLWMGFFDHNKFPARVHRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KK71</accession>
    <name>E3KK71_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_10855</name>
    </gene>
    <organism evidence="3 4">
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        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84696.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329115.2">
        <property type="nucleotide sequence ID" value="XM_003329067.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KK71"/>
    <dbReference type="GeneID" id="10526663"/>
    <dbReference type="KEGG" id="pgr:PGTG_10855"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10855"/>
    <dbReference type="HOGENOM" id="CLU_072384_0_0_1"/>
    <dbReference type="InParanoid" id="E3KK71"/>
    <dbReference type="OrthoDB" id="2506814at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041320">
        <property type="entry name" value="CxC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18802">
        <property type="entry name" value="CxC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxC1-like cysteine cluster associated with KDZ transposases" evidence="2">
        <location>
            <begin position="143"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP84696.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="332" mass="38553" checksum="7DA9ED5989847DE8" modified="2012-04-18" version="2">MTIKRIVVCGTDRTQQVRRRIYAGRIASNLSRLHSAENADRQRAQRPMSEPNENVMDHPTGSFHVFQDNEQEDREDINEDEHEDKTEQAPVWVDLIEEQPDDIDDLIAANKERHRQQARDFNWAVLINYLLPVYMRLRIVTKNWSSTNSYDTFSSCLTTCSKKYNRLVDLQRKQVTFCECTMDGVRLLQQGYLAGSPVKPQTAFSLPLLIFHNSLWNHCHIGAQPFTLALNDWLEPRSQRLCARKGKHPRQLRKPFSAAVDMFRQLEEKRKKSIFSVLGLSNQEFVLMATSSTVTKSKLVAITKNYAHLNFSSARRMSILSHKKFETKKLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLB0</accession>
    <name>E3KLB0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Electron transfer flavoprotein-ubiquinone oxidoreductase</fullName>
            <shortName evidence="5">ETF-QO</shortName>
            <ecNumber evidence="5">1.5.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_11254</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Accepts electrons from ETF and reduces ubiquinone.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a ubiquinone + reduced [electron-transfer flavoprotein] = a ubiquinol + oxidized [electron-transfer flavoprotein] + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24052"/>
            <dbReference type="Rhea" id="RHEA-COMP:9565"/>
            <dbReference type="Rhea" id="RHEA-COMP:9566"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16389"/>
            <dbReference type="ChEBI" id="CHEBI:17976"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.5.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="5">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <dbReference type="EC" id="1.5.5.1" evidence="5"/>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP85085.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329504.2">
        <property type="nucleotide sequence ID" value="XM_003329456.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLB0"/>
    <dbReference type="STRING" id="418459.E3KLB0"/>
    <dbReference type="GeneID" id="10527065"/>
    <dbReference type="KEGG" id="pgr:PGTG_11254"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11254"/>
    <dbReference type="HOGENOM" id="CLU_068105_0_0_1"/>
    <dbReference type="InParanoid" id="E3KLB0"/>
    <dbReference type="OrthoDB" id="437331at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004174">
        <property type="term" value="F:electron-transferring-flavoprotein dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022900">
        <property type="term" value="P:electron transport chain"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:001000">
        <property type="entry name" value="Electron-transferring-flavoprotein dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040156">
        <property type="entry name" value="ETF-QO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049398">
        <property type="entry name" value="ETF-QO/FixC_UQ-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10617">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN-UBIQUINONE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10617:SF107">
        <property type="entry name" value="ELECTRON TRANSFER FLAVOPROTEIN-UBIQUINONE OXIDOREDUCTASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21162">
        <property type="entry name" value="ETFQO_UQ-bd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54373">
        <property type="entry name" value="FAD-linked reductases, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="3 5" id="KW-0274">FAD</keyword>
    <keyword evidence="2 5" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="5" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="ETF-QO/FixC ubiquinone-binding" evidence="6">
        <location>
            <begin position="120"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU366068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF21162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP85085.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="241" mass="27322" checksum="34C93002205F33C2" modified="2012-04-18" version="2">MKASSKSMKFLTARLAFYPPQMNNKGNYIASLSKVRWWLAKQAEALGVEIYSGFAGAKLLHSDDGKAVQGVITNDISLDRSRKPKDPEWSSMLTGSCWPRAAMTAKTPRPTRDGKDPQTYGLRIKEVWRVKDDVYEPGKVLHTLGWLLQRDTYGGSWIYHLEDNMVWLLVWIIQTHTCLLIRNFNLEDELGKTGNGSDLKAGLITGLGLFAWKQFNWTFGELVVRKFCNPGNLDLAKEMVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLK3</accession>
    <name>E3KLK3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11347</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP85178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329597.1">
        <property type="nucleotide sequence ID" value="XM_003329549.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLK3"/>
    <dbReference type="STRING" id="418459.E3KLK3"/>
    <dbReference type="GeneID" id="10527161"/>
    <dbReference type="KEGG" id="pgr:PGTG_11347"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11347"/>
    <dbReference type="HOGENOM" id="CLU_1161625_0_0_1"/>
    <dbReference type="InParanoid" id="E3KLK3"/>
    <dbReference type="OMA" id="RCTEVQV"/>
    <dbReference type="OrthoDB" id="2499957at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173969" evidence="1">
        <location>
            <begin position="20"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="239" mass="24360" checksum="22BC68C2F33746E9" modified="2011-01-11" version="1">MSPSQSIIAFLVLSACALAVPTPLAILKTNAVAQAGVTAAICEALDCEAPGAKVVQDACVAAGLSVGTKASAVAVSPQSPSHIAASAFAGVMNGGNSVAASLYHVVSGVDGAATSVFNTLSAGLIIPALTDVSNKLNGVVATVGEVKHCTEVQLEAATYQGFVTLAMALQSVFWRISHLPTLYVRAESRASLHASLLQLRAALTIFVDHLFNFISADLDKINLRAALFLSLDGAIQACA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLM5</accession>
    <name>E3KLM5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_11369</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP85200.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329619.2">
        <property type="nucleotide sequence ID" value="XM_003329571.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLM5"/>
    <dbReference type="GeneID" id="10527183"/>
    <dbReference type="KEGG" id="pgr:PGTG_11369"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11369"/>
    <dbReference type="HOGENOM" id="CLU_318606_0_0_1"/>
    <dbReference type="InParanoid" id="E3KLM5"/>
    <dbReference type="OrthoDB" id="2511772at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="146"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="250"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="322"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="459"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="654"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="815"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="768"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="165"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="322"/>
            <end position="331"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="459"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="830"/>
            <end position="839"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="886"/>
            <end position="896"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP85200.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="913" mass="97453" checksum="4611DD7FB36A57A8" modified="2012-04-18" version="2">MSSSPPSEPREELPMIGGPTQDLTSPVASAPATVRKPVVVVKKTPIGKTSTGKDLFDTIELMKGLKRVEVKQSEGSRTGAGGLEKSGDLGDSVLVTSDKWTKIQAKRITAPKEQSTITPTKALTFGEKKLAAMKKNLTAVQYRTTPQQGIALDPQLSGDEDGNKGKKKKASPAKKKKATAISLPTDVDALEPVGSSAVPVEEKAPAAKKKKAPCLPLVQDAQGPVAGAAVTGKKNLDLVFQPASLAKTKKTTGNCSAPVEKAAGSVGQPGGPGGKDIPKKSLAPQPDLPGSADLASKDTAQVGEHAEEPCLATVNKRLDVDGGRDTLERTRTGVAVGESGAPAKTTISKGPPDDVGQSTHLASQLAVPAVPLSQPALEKFTGVGLDNSTQDGLDTVGWSAGVSVGLAGMVGNNAAMVDRPDQPEPSMDLEDFTATQDGLMRMLSGLETLTPLPANQLHARAAHAASGDDVQDQFPDQDGGDPLDPSWSKRVEQALARKRAQEYCALAKAEQEEQEVQNQKAWRMMVERYLGIRPVGEESGPGVEFLVGLVPPLDNDQILVLQEVRSWDKINGLELGFMRWEWEGGMDDDLMRPVPTWLIKDGVEVKCFIDDAAHERLARFMGNQPWDFYQLPICYGSAFASRDEWEKAVKNDTLLQPDSPIPDEDIETTATASPDTAVANGSLTLNELYQKIFNPTKGRNVHRFRYESTGPGTPCRRDDGLTSLEDLKLLLGIWSRTKFGLKIDLKMLKDKGLHTASPKLFAENLKRLDDISTYIDDLKAQIRDCEANKTTAVKKVVDLKRAEVLQPTKRKHVDVEVQDVTAPPKKKTKAAPAVAVQTADPPLKKKTKAPSEVATPKVAAAAKKKTTKAPGGSEPKKLASKKTNGRAGGLESGLGLQGTPQGQGSNILPSYIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLQ1</accession>
    <name>E3KLQ1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="8">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_11419</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="8"/>
    <dbReference type="EMBL" id="DS178294">
        <property type="protein sequence ID" value="EFP85250.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329669.2">
        <property type="nucleotide sequence ID" value="XM_003329621.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLQ1"/>
    <dbReference type="GeneID" id="10527239"/>
    <dbReference type="KEGG" id="pgr:PGTG_11419"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11419"/>
    <dbReference type="eggNOG" id="KOG2204">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013086_0_0_1"/>
    <dbReference type="InParanoid" id="E3KLQ1"/>
    <dbReference type="OrthoDB" id="8118055at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000130">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050749">
        <property type="entry name" value="Glycosyl_Hydrolase_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742:SF103">
        <property type="entry name" value="ENDOPLASMIC RETICULUM MANNOSIDASE MNL2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="8" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="24"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="140"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="322"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="456"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6">
        <location>
            <position position="542"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="588"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="674"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP85250.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="747" mass="85159" checksum="2FDE59DAF97D1C79" modified="2012-04-18" version="2">MTTKSGRNLISSGIGAYNPVRSRFRAFLALILTSGLVLYYYLNSHWPLAHESARRPVDQGVGHSPKGTHGLGPSMVEEDDQARIDQNKTYTPEELMKLPVLHHTFEDPFGPRNGFKALRDSKAPWQATTPTGAVLINHLSRNRFPPPDSIDSSRGSSPPPVPIWPSSTLLLEKEESLKSPIPTHKIAGLNASYVWEGRDRIKEGELPKVQWPGFEKPGWEAPADRTIRLERQGWVRRGFQHVWEGYKARAWAHDELKPVSGSFQNPFGGWGATLVDCLDTLLIMNLTLEYNYARTHVKAIDWAHTIDKDPLISNHPKISFFETVIRYLGGLLSAYDLSGDKLMLQRAEDLAEWLLPAFGTSSGVPETHYQMGFNPLGGKVGNVMTSDIGSLSLEFTRLSQLTSKGVYYDVAHKIIDLLDGDQWVSDSRLGTLFPTVINPDSPKSLGNKYTFGAMVDSYYEYLIKQYQLLRGTDLQYRKMYTSALMSARQHLIRTYDLETSGGAMIALGAQLLHQKEDLDLALRHADACVWAYESTKTGLGPEEMWISKGNEHTQWQPVLYKGKIFRELKPEPVRGAWIGDPSYFGRPETIESVYYMWRITGDRQWQDRGWRMFTSWMEACVTKFGFANLEDVNSWPPKTTDKQESFVLAETFKYYYLLFSEPDLISLDDYVFNTEAHPFRIEIPGKPAKRYWTKPDESLHKMYDPPELKDDEQGYGTFLQQWARVDLDKLSQADRQVYNRVMGIKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLW5</accession>
    <name>E3KLW5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Chitin-binding type-4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11483</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178294">
        <property type="protein sequence ID" value="EFP85314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329733.1">
        <property type="nucleotide sequence ID" value="XM_003329685.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLW5"/>
    <dbReference type="GeneID" id="10543857"/>
    <dbReference type="KEGG" id="pgr:PGTG_11483"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11483"/>
    <dbReference type="HOGENOM" id="CLU_1435075_0_0_1"/>
    <dbReference type="InParanoid" id="E3KLW5"/>
    <dbReference type="OrthoDB" id="2496825at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172715" description="Chitin-binding type-4 domain-containing protein" evidence="1">
        <location>
            <begin position="26"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="189" mass="20762" checksum="B2858E16FFC1C2C2" modified="2011-01-11" version="1">MLLATSIRWFTLAVLAVVDLRSVQLAEPHPHNLLPKRDGGADPVTAPPQFSHANAVRCSQNFQIGGRDGIAQGKTRCENDQGWRYECPTGHCHGGGPNNHPNDHPLSGFLFRGCKLQSDYHKPGPGQITVNPDSFGIVPGMKQLMVWGYAVPAGSTTPSKKRVGYTCDWNLDTDNNARRPWCNFCIKIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPJ2</accession>
    <name>E3KPJ2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Peptidase M12B domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_12183</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="DS178299">
        <property type="protein sequence ID" value="EFP86227.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330646.2">
        <property type="nucleotide sequence ID" value="XM_003330598.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KPJ2"/>
    <dbReference type="FunCoup" id="E3KPJ2">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KPJ2"/>
    <dbReference type="GeneID" id="10540238"/>
    <dbReference type="KEGG" id="pgr:PGTG_12183"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12183"/>
    <dbReference type="eggNOG" id="KOG3607">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012383_2_0_1"/>
    <dbReference type="InParanoid" id="E3KPJ2"/>
    <dbReference type="OrthoDB" id="5951731at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.390.10">
        <property type="entry name" value="Collagenase (Catalytic Domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.70.10">
        <property type="entry name" value="Disintegrin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001762">
        <property type="entry name" value="Disintegrin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036436">
        <property type="entry name" value="Disintegrin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024079">
        <property type="entry name" value="MetalloPept_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001590">
        <property type="entry name" value="Peptidase_M12B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905">
        <property type="entry name" value="ADAM A DISINTEGRIN AND METALLOPROTEASE DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11905:SF159">
        <property type="entry name" value="ADAM METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00200">
        <property type="entry name" value="Disintegrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13688">
        <property type="entry name" value="Reprolysin_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00050">
        <property type="entry name" value="DISIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57552">
        <property type="entry name" value="Blood coagulation inhibitor (disintegrin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55486">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50215">
        <property type="entry name" value="ADAM_MEPRO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50214">
        <property type="entry name" value="DISINTEGRIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172123" description="Peptidase M12B domain-containing protein" evidence="3">
        <location>
            <begin position="26"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M12B" evidence="5">
        <location>
            <begin position="336"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="Disintegrin" evidence="4">
        <location>
            <begin position="579"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="229"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="640"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="494"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="493"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="497"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="503"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00276"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50214"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50215"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP86227.2"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="670" mass="72236" checksum="35A2802484ED72CB" modified="2012-04-18" version="2">MVNFFKFSILLQSICICLIIRSSEGSSDRKPIGRVVYASDFEIQVIPTWSLGSYNDEQMNFIGPTRGMAMDQETSSICSGNSILLKFSIPRSSPQSSKVGQQEDGHEDSKHRFALYLEPDSTQFLHPNATIITYQSSNGSSTHSNQQLPISRPIIADEVLAYSGTAIDDNDPKSLDDSYHLRFNNWANVLIHHPYGTKCVRNSDDLILEGAFQVNGRLWHIKSLDSYQKSKGPYDSQPNELSSAPELKHNQVREGTDFSYQKESIRQQASREKLSSRVISNSTETSSLQITYTESSTQHYLQKRQTMTSDIAGLTGDKVNYASTIGSTDGCPKSPKMVYIGIAVDCTYLRKYGSTDAVRLAVLNNMHLVSSVYQRTFNISLGIVEINMPSESTCPSQPSSDRPWNVDCPDASSQGLDINERLNKFSKWRSDKGGSDGAGLWHLLTDCPNGSQVGVSWLGSLCKVNAEQGANGGTTSGTGVTASTPNEWETMAHEIGHNFGAVHDCVSGCSMEDSCCPASTSSCNNGDQNIMAPSSTSGSTGTFSACSIGNICSNFKPGNSQRMDSSCVADLGQHYTISLNQCGNGILEPGEDCDPGQTFSPCCQWGVCKFSPGSVCMPSANSPCCSTSCQFAPASTPCNSSESLSATCSGDSPNCPSGTGSENPSFSSSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPP7</accession>
    <name>E3KPP7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_12238</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003330653.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KPP7"/>
    <dbReference type="GeneID" id="10540295"/>
    <dbReference type="KEGG" id="pgr:PGTG_12238"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12238"/>
    <dbReference type="HOGENOM" id="CLU_1215311_0_0_1"/>
    <dbReference type="InParanoid" id="E3KPP7"/>
    <dbReference type="OrthoDB" id="10549638at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP86282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="228" mass="25468" checksum="229FA8144868CE31" modified="2011-01-11" version="1">MCSSINYKAIQGIILAGPIAELNPDVIRQHFPPHIIRLMVDQLQEQFCPSKTFKRKRAERVGQKVRHVLLVKDNGDVMEGLVLLNKQEYVDLIQATENAMEKLGVKKPGQEDMAAESDDDHGVLLTQGEHEAFMEKLSAVGYRLGELNQNQVVRVQTSLTYSSGRRERVTFGAYQGPAGLLIAHDQFSKFFINSWSGYSMAKSINETSTSLMDSNQLHNPKFCLGTAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQ85</accession>
    <name>E3KQ85_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_12416</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178300">
        <property type="protein sequence ID" value="EFP86460.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330879.2">
        <property type="nucleotide sequence ID" value="XM_003330831.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQ85"/>
    <dbReference type="GeneID" id="10540475"/>
    <dbReference type="KEGG" id="pgr:PGTG_12416"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12416"/>
    <dbReference type="HOGENOM" id="CLU_141179_0_0_1"/>
    <dbReference type="InParanoid" id="E3KQ85"/>
    <dbReference type="OrthoDB" id="10376559at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP86460.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="158" mass="18218" checksum="D9D786D8D26574D4" modified="2012-04-18" version="2">MNVIQTQHLCPNHLVSSTLELKKPKRPAAHRVLDIVPATMGSSRMVMRRFTNSSRESECHLNDPDPDIHWTSEFDRLFPGRLFMGRRDPSVLRTMKVPTILVGYNVNKWRLLDDCYTTDGETLTFPCGPSGCWDAGFLPEFWERRGISRSLLWCILLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQC4</accession>
    <name>E3KQC4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12455</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178300">
        <property type="protein sequence ID" value="EFP86499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330918.1">
        <property type="nucleotide sequence ID" value="XM_003330870.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQC4"/>
    <dbReference type="GeneID" id="10540514"/>
    <dbReference type="KEGG" id="pgr:PGTG_12455"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12455"/>
    <dbReference type="eggNOG" id="ENOG502SCJN">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032241_1_0_1"/>
    <dbReference type="InParanoid" id="E3KQC4"/>
    <dbReference type="OrthoDB" id="2507256at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="268"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="446" mass="48665" checksum="B51D0FDDE1FAD950" modified="2011-01-11" version="1">MIAYRAEQVQLKKMKAQEKAKQKRLRSRGVGQARRTTGQRSSANSSTPGINPGSTQMASQPLDSDSNPQFGSEDYENVCGYLEEEVNYTRLYGDGSKTSVGTTKVTKAAAYEMFAIFINDNSNRRLNLTGSQLRQRIDGYKKRFMKAKEWAENTGAGIEEGDNLPTLAEILEKKCPCYDRMYGIFGGKANVTPLAQFDSGVGADLYMTADDTDNPQDPASSPEVFFPGWDETQEEPPPSGLTTPAEQQESPRGPGLHEGAADLSLPTLDGDLSLPDDILPPPLEFSGTPLTPSPGLNSPRSLAGVNGRSSGAAFGHPNGQTTTPDSPHPVQPGRRAFPNQRSTDASPAGPARGVNPRARSTLASAFENSNSEKFVYLKEHMEWEKQKEEKRLTWEKERYNKEAALAKDGRQGQLRLAESKMKAAREWISQGKTAAEVEGLLKAVYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQQ0</accession>
    <name>E3KQQ0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_13007</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178301">
        <property type="protein sequence ID" value="EFP86625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003330996.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQQ0"/>
    <dbReference type="GeneID" id="10540886"/>
    <dbReference type="KEGG" id="pgr:PGTG_13007"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13007"/>
    <dbReference type="HOGENOM" id="CLU_012972_0_0_1"/>
    <dbReference type="InParanoid" id="E3KQQ0"/>
    <dbReference type="OrthoDB" id="2501372at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP86625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="464" mass="52444" checksum="C578EA512716AD2F" modified="2011-01-11" version="1">MSSLVSGPLALFQDLFFRQSCSCQSRLSAAIRIVKIGLAVQKLSASKVNTRIFAYLVSGALYRVTCSFSSIKLSSFWVILFYTLRGVYCGGLRTKKSINRDRMADNEVGAAMVKIKPQNKSLAFNGSNIECFLSQYQLAARLDGASEKDMAQQLGFFIAKDELLDVVETLEGYEPPDWPKLKASMIAYWGNVDTAKFTSRDLESLVEEWKSKGGISSVVDYQEFRKTWEPIQSYLLAKAHIDSVEEIHKWYYQSFSTGVQERIRDQLIRDKTMITTLDKRFKLPTFEVLKNAVEEVMKGQTALTFEDSRSTVPVPSTLFKDSNDVMKKMEADRRPKEVSDASKPPATIDEISKMLQSFEQRLKKELSTPQASQTTPSGTRPPMVCYYCHYEGHGTGRCQKLQEDKENKLVEQQGNNFFLPNGALIPFDRTQPIRHVVASYQPPIPSASFVSTEFKTSCGTLQPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQX9</accession>
    <name>E3KQX9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13086</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178301">
        <property type="protein sequence ID" value="EFP86704.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331123.2">
        <property type="nucleotide sequence ID" value="XM_003331075.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQX9"/>
    <dbReference type="GeneID" id="10541127"/>
    <dbReference type="KEGG" id="pgr:PGTG_13086"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13086"/>
    <dbReference type="eggNOG" id="ENOG502SPG0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013565_2_0_1"/>
    <dbReference type="InParanoid" id="E3KQX9"/>
    <dbReference type="OrthoDB" id="3252425at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052717">
        <property type="entry name" value="Vacuolar_transposase_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46169">
        <property type="entry name" value="DNA REPLICATION-RELATED ELEMENT FACTOR, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46169:SF15">
        <property type="entry name" value="INNER CENTROMERE PROTEIN A-LIKE ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86704.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="236" mass="27051" checksum="120C5DF40F29E2C6" modified="2012-04-18" version="2">MHFPTDSDRLARGDKTSPEEYEYSSDGESEVEPAQEDHDTLSDTDIDNASDEGDKDRYTSTSCKETLAKFWAIAKKLRYSPNSKAEFVETCRDKECATPHMVERDVRTQWNSTAAQLRSVIRCEAAILEWQRHKRHGMKRKYFVDQNDFDLARDLVEVLDLFHEITLQISTAGSTQISNVVVFIDQITDHLSTAIINPDYPPALRNACRSGLKITNKYYSLTDASPLYRIAIFRDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>E3KRD0</accession>
    <name>E3KRD0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_13237</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
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    </reference>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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            <dbReference type="PubMed" id="21536894"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP87286.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
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</entry>
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    <accession>E3KSL3</accession>
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        <recommendedName>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="655"/>
            <end position="675"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="687"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="domain" description="Cas1p 10 TM acyl transferase" evidence="10">
        <location>
            <begin position="314"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="764"/>
            <end position="800"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="786"/>
            <end position="797"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP87299.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="893" mass="102244" checksum="DDF43C9E24B26EA4" modified="2012-04-18" version="2">MLSTLRSISQSTNLTLFHLNCSLIAALLGLATYRLFIKDWSDPNHCQALLHTGDWLDGSRHTNWQPTGCMLHTYKPKSVGQCLAGRSVVFIGDSITRVLFYGALRTMDPSVTPDGQSKHSDRTLKIGGIEWRYVWDPFLNSTTWPEIISGNYLSSLPSPTSNSKEDLALKNRPALLVVGSGLWYLGLPSGIDTWKQKVDHLFQITSPRLAHKQARIADEIVLLPVEYPAFEKLNQDRKQRMHPDQVQKMNDYTLKKSNSSPSSPIVIPTVMNLISQQAPSETQDGLHYSNKLSDIQARILFNLHCNDQILKKFPLDKTCCFQYPPPNWIQIILLLFLLVWAPLGIYINHYPNSPLAVHSYLFPSNDRLNSMAIFGGSIVLIFLSDRTSMFNKEQKQFDRLQFGTLNLLALVAGIWSAVTSEKGDMGLLNREQTDEWKGWMQIAILIYHYLAASQVSGIYNPIRICVASYLFMTGYGHFTYYYLKKDFSFPRILSVLVRLNLLTLVLAYVMDTDYLSYYFSPLVSMWFLIIWATMFIGHQWNDRMVFLLPKLACSVVLIVWFFKADKPLGWVFGFINLVFGTEWNANEWRFRVTLDMFIVYWGMLTSLIYLKVKEHKWLERDHSNRFDQLRKIAIWISALGLVWFFWFEISRPNKLIYNLYHPYISIIPISSFIILRNSTEFLRATNSKFFVFIGQCSLETFIIQFHFWLGADTKGILKVLPFRGHRLLNLIVSTVVFIFVSHQVAQASGTLSSWLCYKPKPSVSTSVGPNDGGSSLEEARAKPETSHQQPPHPGSSSVNQHDSYLPLSTNDIDKDVHLSLSDSPRSSQQPSIFLQAIDSLRNLYRSQLPFRALVWLACFWILNLLTPSKVNSHGHIRLSLSRSLSLLFLDVPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3KTL1</accession>
    <name>E3KTL1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Translocon-associated protein subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_13504</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Is probably involved in a pathway contributing to genomic integrity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the IRC22 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178308">
        <property type="protein sequence ID" value="EFP87718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332137.1">
        <property type="nucleotide sequence ID" value="XM_003332089.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KTL1"/>
    <dbReference type="GeneID" id="10539116"/>
    <dbReference type="KEGG" id="pgr:PGTG_13504"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13504"/>
    <dbReference type="eggNOG" id="ENOG502S7BF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_077471_0_0_1"/>
    <dbReference type="InParanoid" id="E3KTL1"/>
    <dbReference type="OMA" id="NNPFNIV"/>
    <dbReference type="OrthoDB" id="1926781at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005595">
        <property type="entry name" value="TRAP_alpha"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12924:SF0">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12924">
        <property type="entry name" value="TRANSLOCON-ASSOCIATED PROTEIN, ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03896">
        <property type="entry name" value="TRAP_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0732">Signal</keyword>
    <keyword evidence="2 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173875" description="Translocon-associated protein subunit alpha" evidence="11">
        <location>
            <begin position="23"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="161"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="194"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="200"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP87718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="254" mass="28090" checksum="74D75E25ADE73882" modified="2011-01-11" version="1">MNFLLSCKNLAALTVLIGSVSSLLTPEKLEISAAFPTNNQFGIVFNGQPNKILLKVLNLGKDPVEDVMRIHQVWNEFREIGGKERLLRKGAPVPSKRTLPPKIEPYIIPYMFSAEEREGNIGLRIWVEWSGPKGKKHQSIAYDSTVTIQEPPTRWFDPQLILLYIILASTFGGIGYMVYSSYVVPVKPARSLKSKRSAEGSLQSSSKVSTPTGGAYEEEWIPAGHSVRSSKKTGTETGGEESSGAEGKARRRKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KTV4</accession>
    <name>E3KTV4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_13443</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178308">
        <property type="protein sequence ID" value="EFP87657.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332076.2">
        <property type="nucleotide sequence ID" value="XM_003332028.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KTV4"/>
    <dbReference type="GeneID" id="10538889"/>
    <dbReference type="KEGG" id="pgr:PGTG_13443"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13443"/>
    <dbReference type="HOGENOM" id="CLU_915677_0_0_1"/>
    <dbReference type="InParanoid" id="E3KTV4"/>
    <dbReference type="OrthoDB" id="10308944at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173887" evidence="2">
        <location>
            <begin position="26"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP87657.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="304" mass="34108" checksum="671158647C2565AD" modified="2012-04-18" version="2">MKAITSLAYLFVIVLVEYNTPWSAAIGLNEICEHSVKLKTDLQGPGEIQPTSLQPQAFLEHSFETKVRIKDDFVYINNPFPGKCYASVKLDDQVLAPLTTLNDINGLNVIPKKELDAIHCVARLESSKPPDSEIKVRYHIDDQLIASLLDGTAGFSRSDRVISPTNSPEHSLSQSSLGSTGTRYIDAPYSWRNGEPSWRSDGEVQGDNTAYPQHSDSLSRDSDFGSHSSEDRFHHHDSESEDSESSSKRNSYYFPEEKDPASEPEEEETASLLHRDASQDGPAKKLKPWSFRRLLKLQNKKENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KW01</accession>
    <name>E3KW01_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_14054</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178314">
        <property type="protein sequence ID" value="EFP88476.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332895.2">
        <property type="nucleotide sequence ID" value="XM_003332847.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KW01"/>
    <dbReference type="GeneID" id="10540779"/>
    <dbReference type="KEGG" id="pgr:PGTG_14054"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14054"/>
    <dbReference type="eggNOG" id="KOG2366">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1525922_0_0_1"/>
    <dbReference type="InParanoid" id="E3KW01"/>
    <dbReference type="OrthoDB" id="2497659at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP88476.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="176" mass="19277" checksum="F8C31F168774C4F4" modified="2012-04-18" version="2">MDSFEQEIAGHGSLIVQLTETKEAPKRDFQRIPAAQAELISPAHFRQVGELKVATSIKAEGQGAVVWHDVPGSQDGHVLLSFDYINAELPSDDEDHAKLNFKRAAITINDDPHRKFQVHFPITGMTWSDVYPGYLVSLPLPNPKNKVRIEGLDGAAPDFVALSVAIQPAPTTTKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>E3KWI4</accession>
    <name>E3KWI4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CSD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_14864</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178315">
        <property type="protein sequence ID" value="EFP88659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333078.1">
        <property type="nucleotide sequence ID" value="XM_003333030.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KWI4"/>
    <dbReference type="STRING" id="418459.E3KWI4"/>
    <dbReference type="GeneID" id="10541448"/>
    <dbReference type="KEGG" id="pgr:PGTG_14864"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14864"/>
    <dbReference type="eggNOG" id="KOG3070">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_754665_0_0_1"/>
    <dbReference type="InParanoid" id="E3KWI4"/>
    <dbReference type="OrthoDB" id="422005at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031054">
        <property type="term" value="P:pre-miRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04458">
        <property type="entry name" value="CSP_CDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011129">
        <property type="entry name" value="CSD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002059">
        <property type="entry name" value="CSP_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051373">
        <property type="entry name" value="Lin-28_RNA-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46109">
        <property type="entry name" value="PROTEIN LIN-28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46109:SF1">
        <property type="entry name" value="PROTEIN LIN-28 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00313">
        <property type="entry name" value="CSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00357">
        <property type="entry name" value="CSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51857">
        <property type="entry name" value="CSD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CSD" evidence="4">
        <location>
            <begin position="70"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="304"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP88659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="367" mass="39094" checksum="AFBF2CB2F608BA8D" modified="2011-01-11" version="1">MSYENTYLTVNSPSFGRLPVSFPSASAMTPPMMPIIGYPGQQMMMASLSPHPQSPMASAPVIDKLNPPPRRTGVCKFFNVGKGFGFIKDSRPEELPVLTGDPGTDIFVHYSCIVSCDAPYKSLLDGEVVEYYLGRSNKGLAALEITGPGGVNVKGCMNSRSPTVLQPMPNFSGPIRMISAPVTFGPNHSPNPSPMASPAFDYNTSLGWSMPMSGAQTLGVHSNTAGYSLAVGMPLALNQHLRRETTESLGVPNNDASAKMYRVSSTPISAPGFGSTHIKGFRESANMANLGVGIGIGVHWEPKSRGKPVQWSPPGAPGEAIGQELSQSHGRRVGQIAATDRTKSNWRGEDIKHRIVSLEINGSRASP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KX47</accession>
    <name>E3KX47_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14156</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178317">
        <property type="protein sequence ID" value="EFP88817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333236.1">
        <property type="nucleotide sequence ID" value="XM_003333188.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KX47"/>
    <dbReference type="GeneID" id="10541931"/>
    <dbReference type="KEGG" id="pgr:PGTG_14156"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14156"/>
    <dbReference type="HOGENOM" id="CLU_785592_0_0_1"/>
    <dbReference type="InParanoid" id="E3KX47"/>
    <dbReference type="OrthoDB" id="10453170at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="72"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="149"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="228"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="322" mass="35258" checksum="A002D7D6F9408CE5" modified="2011-01-11" version="1">MGYSPPALHYGQLAVAAPRSVTQTFRVKPNPHATSAAATTQSMKQQITLLESWLQLWSHHKLHHHCLLNSPLRSTPESSSSSATAARQAPPSTSPLGSTKKQPIEDYNQFALLVSSTYDPNQTVQLPETPEEKTGSYKEQRAAKIDHQSSSISPSTSALSSGGDSSGSSSPGLARSSSFYRRLLYSFDKTLTQLVLSHHHQSFPSLLVSPSEAPALIHPRPRPPSPSSSPSAPAQRRQAPRPLEPDKHNPLFPPDLDGPICPTLKRKSILKPTTTLDHHGFVFNIQLNHLPSILHPHLPSDHIRLIEDHVLSNFVYSVKTRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3KXP1</accession>
    <name>E3KXP1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_14944</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178319">
        <property type="protein sequence ID" value="EFP89103.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333522.2">
        <property type="nucleotide sequence ID" value="XM_003333474.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KXP1"/>
    <dbReference type="STRING" id="418459.E3KXP1"/>
    <dbReference type="GeneID" id="10546572"/>
    <dbReference type="KEGG" id="pgr:PGTG_14944"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14944"/>
    <dbReference type="HOGENOM" id="CLU_1289502_0_0_1"/>
    <dbReference type="InParanoid" id="E3KXP1"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050886">
        <property type="entry name" value="RNA-binding_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48024">
        <property type="entry name" value="GEO13361P1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48024:SF56">
        <property type="entry name" value="HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN A0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="RRM" evidence="4">
        <location>
            <begin position="21"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="167"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="201"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP89103.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="214" mass="22405" checksum="BFC7C31927698A58" modified="2012-04-18" version="2">MNSNRPENYSAEGQDAAQMSCKIYVGNLAWATDENALSSHFASVGEVKGAVVMRSEGRSRGFGFVTFANPEQAKQAIDTMYDSELCMSLPCVDGRRLRVNVANQLIRGGTGGPGDYPMSSGSAPGGYQYPNAPYPGGSIPYGGYSHPPPPAHGGYMGGGYMNPTSSYPGNETGGYPTGGPTSHPMPDGRKGNNQPGGFYPNGGSYPYPQGGYGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZ83</accession>
    <name>E3KZ83_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">G-patch domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_15571</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TFP11/STIP family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178324">
        <property type="protein sequence ID" value="EFP89608.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334027.2">
        <property type="nucleotide sequence ID" value="XM_003333979.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZ83"/>
    <dbReference type="FunCoup" id="E3KZ83">
        <property type="interactions" value="675"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KZ83"/>
    <dbReference type="GeneID" id="10547657"/>
    <dbReference type="KEGG" id="pgr:PGTG_15571"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15571"/>
    <dbReference type="eggNOG" id="KOG2184">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007977_2_1_1"/>
    <dbReference type="InParanoid" id="E3KZ83"/>
    <dbReference type="OrthoDB" id="4822at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071008">
        <property type="term" value="C:U2-type post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000390">
        <property type="term" value="P:spliceosomal complex disassembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000467">
        <property type="entry name" value="G_patch_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022783">
        <property type="entry name" value="GCFC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022159">
        <property type="entry name" value="STIP/TFIP11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045211">
        <property type="entry name" value="TFP11/STIP/Ntr1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23329:SF1">
        <property type="entry name" value="TUFTELIN-INTERACTING PROTEIN 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23329">
        <property type="entry name" value="TUFTELIN-INTERACTING PROTEIN 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01585">
        <property type="entry name" value="G-patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07842">
        <property type="entry name" value="GCFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12457">
        <property type="entry name" value="TIP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00443">
        <property type="entry name" value="G_patch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50174">
        <property type="entry name" value="G_PATCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="G-patch" evidence="8">
        <location>
            <begin position="268"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="316"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1003"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="91"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="121"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="155"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="193"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP89608.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1042" mass="117102" checksum="17626DB205BC4026" modified="2012-04-18" version="2">MARKREVIMDDSDLDSNSDSSGNEQDQIEFDDRDLQDEHSLFDDPYRNKGNKRRRMNDGKADAIYGVFAAEDDQPTKKTNKPDKRLHRTQAFVQASSSSSNLPNQTENRSDGEQEPSQDSSTDHDSGSDSSDSKSDSIGETENPNPPALSETATSGLGSRHGLGSSASNSDFRAMLGATGGLGSRGLGSQAANRSSSTTSGHNGLGSQPSDETGNESRNSDQNTPEPTVPRRSFLGAQQANPSIQPVKKKPLSKAEQQHFAKLASNKTSSIGLKMLQNMGWKSGTGLGTQGEGIVTPLETKARPKGMGLSYKGFQERTAQAKEEDRRNGKIIDEDDDAKKFGKKKDKKGKEIVREAWKAKPKTPKKAKVVHRTYEQIISELDPDQTSIDPGVGEIIDLTGRKLPSLNTTLTHHSKGPTEEEQEHRLPELMHNLSLICDISKGSLIHLAKEGQAIKQKSQQLTKDQARSEQILKLQQQKLSKIKRIVELTRLTKGTYTNIISQITENSSALEICQMIDQFDELFFELLEFLSDNPKSDQESLRLDEIVVSALAPILRQVWSRWDVLRQPGLSVKELKRFRKCFRTDQEGSHSSSPTGDEESFQHPLFPTLSRGSAMQSNRKMTAYESLIWNDWLPKIRSAVNVWSPYQSEEMITIYTSWKSILPPFVHDNFLDQLIVPKLLTWIGNWDFREQTEAFDSTRPDTKRVSLHLFVFPWLELVGAYRSELILAEVKLKLSAWLKSWKYTSVESAEEGLMIQDLLVWKKDVLKRRDWDKMILKTVAPHLASYLRSNLSINPKNQNLRPFQVMLNWCQLITNQEILDQLLTNEFFSKWIDTLWIWLTSPSPNGDQIGEWYNWWKSLFPEYLLSDRSIVSLGFATGQELMNQAKSLSSLGGDLKAQLPKPDLATITAALASSSGSRRKSKKEDHQQSKLKKSVGNRLEEITFKSIVEEEVQKKNLFLIPLGKTLDSNGRSLFKVSNQFAPHSSGLIISISDDVVFVKNHSKKSSLEHSHPPNVDGDGEDWEPMLLEDMLEKALVVINPST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZQ4</accession>
    <name>E3KZQ4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_15435</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178325">
        <property type="protein sequence ID" value="EFP89779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334198.1">
        <property type="nucleotide sequence ID" value="XM_003334150.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZQ4"/>
    <dbReference type="GeneID" id="10545430"/>
    <dbReference type="KEGG" id="pgr:PGTG_15435"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15435"/>
    <dbReference type="HOGENOM" id="CLU_2211273_0_0_1"/>
    <dbReference type="InParanoid" id="E3KZQ4"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP89779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="107" mass="12130" checksum="1B3470AA4AF4A935" modified="2011-01-11" version="1">MLLPSHKGLINRRRERDLNNLFGIHKEPVIESQALLQNPVILVHVKKVNRFVGFGVLGAKPQGQIGVAGIEREENYWAGISICKNWTMQQLAETLPRRNTRLGAIYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZV3</accession>
    <name>E3KZV3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15790</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178326">
        <property type="protein sequence ID" value="EFP89834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334253.1">
        <property type="nucleotide sequence ID" value="XM_003334205.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZV3"/>
    <dbReference type="GeneID" id="10545566"/>
    <dbReference type="KEGG" id="pgr:PGTG_15790"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15790"/>
    <dbReference type="HOGENOM" id="CLU_039761_2_2_1"/>
    <dbReference type="InParanoid" id="E3KZV3"/>
    <dbReference type="OMA" id="LECARYC"/>
    <dbReference type="OrthoDB" id="2496728at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058913">
        <property type="entry name" value="Integrase_dom_put"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177:SF1">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24764">
        <property type="entry name" value="rva_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="179"/>
            <end position="370"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="473" mass="54861" checksum="C5FCED6207C9100A" modified="2011-01-11" version="1">MRITLQNFGHEFQSIVSELINAGHNDNEIRQFLQENHSIIVSQHTLTRRKEDWGLILHASQQMANTEEHIKKYFDQGLTYSQIHHALTTSHNYTHSKRTLQRKITAMQLSRRLDDLDTARVTIEAVVSCVMHLHLTPEGRNVGYRRMRQLLQTKFGITLHYITVALINPKRVLKRRVFKTPGPNYIWSADGHDKLKKFGITLYGFIDAWSRKILGIYVHITNNDPRHIGYYYLQLVKETGGIPRRTSTDKGTETIHLAGHQINLTQQYNEESIDPTQSHLFTKSTHNQKIECLWSQLMKQYNSELINKLFTAIEESFYDPQDPLEQLLFIYLWVPLVQRSLDDWTNNYNTYKRRLDKKSSLPTRCSADWCFNYPEEQGGEQGLIKVPSEAADALEKEFYPEGDELLRTTPKWFSDIIAELQAAFDLAIPIVTVHNVWEVFTVLNKAIQAYDTAWLSDPSNDPSLSIAARCLET</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3L120</accession>
    <name>E3L120_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_16523</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178330">
        <property type="protein sequence ID" value="EFP90245.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334664.2">
        <property type="nucleotide sequence ID" value="XM_003334616.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L120"/>
    <dbReference type="STRING" id="418459.E3L120"/>
    <dbReference type="GeneID" id="10546387"/>
    <dbReference type="KEGG" id="pgr:PGTG_16523"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16523"/>
    <dbReference type="eggNOG" id="KOG3010">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049344_1_0_1"/>
    <dbReference type="InParanoid" id="E3L120"/>
    <dbReference type="OrthoDB" id="10027013at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000852">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051052">
        <property type="entry name" value="Diverse_substrate_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942">
        <property type="entry name" value="METHYLTRANSF_11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44942:SF4">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 11 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="4">
        <location>
            <begin position="43"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP90245.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="287" mass="32529" checksum="ACE00647F8559F3A" modified="2012-04-18" version="2">MAETFATPSYSVENYASYRPSYPPELYQAIMDYHQASGDLAIDLGCGTGIVSAELVRQGRFDRVVGVDPSKPMLEYAKKMITNAEFVHGRAESLPWITNNSVDLLVAGTSAHWFDTTWWKEAGRVLKPQGTLAVFIYGGLWPDPGHQCAEELRATMFSFAEELGFSSIGNRICHHMYDELPLPDLTDKGNLGDFQRIDWNRHGLGDRLVMADRIKLRAWRERARTYGPAHRWQIENPTKIGDKILDPVEKTLTTMMSILETEDEEMEIVCGHSLSLLLFRRRAVCTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3L1V7</accession>
    <name>E3L1V7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="5">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_16558</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="5"/>
    <dbReference type="EMBL" id="DS178334">
        <property type="protein sequence ID" value="EFP90532.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334951.2">
        <property type="nucleotide sequence ID" value="XM_003334903.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L1V7"/>
    <dbReference type="STRING" id="418459.E3L1V7"/>
    <dbReference type="GeneID" id="10530429"/>
    <dbReference type="KEGG" id="pgr:PGTG_16558"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16558"/>
    <dbReference type="HOGENOM" id="CLU_011478_0_1_1"/>
    <dbReference type="InParanoid" id="E3L1V7"/>
    <dbReference type="OrthoDB" id="2503239at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="117"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="483"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="546"/>
            <end position="556"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP90532.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="580" mass="64369" checksum="54ACFE627F8D62AD" modified="2012-04-18" version="2">MNRSETEHSTPIADDSSHEEDSDSGQSIAASNPIEDDSDYGERITESEVIDEIAALNCGESLPDELASLKLQATSKKRVTLRQDILELNHHELKEHIRVESRNLYGDEAKDEQLKAVAALVHDRHTFVLAGTGFGKTRITEMYHNLFQPYQKSIVLVLNPLDSLGDNQVEEKRKVGVNNRHIKAVNLTSKVLNAKTARKIISGKFEFVYLSPEAFLNSEIFRDVYFNPKFQSRLSLIVVDEAHMVYVWGLVANGQSKGLTSHLKHGERGVFRPGYGELAARLMATNGTPLLMMSATCRPIAINSILESFKLTRQMVTFVEAELTRPEIRMLRINMQKSLVSSEDLADLYSTQEQTPDNEIIPTLIYSTTRNLTGQVLDVIHNAREANQKDDPFSTFARRYHSITGDMVSDSRDSETRSRVSSSMALSFDMSDARTCWRDGRKGVAIMFVEPHRQNGKNSVSDFTNVELQNDDDRMDAVVTGARLGQSFGRGKSSFPRRGKAGKNRPNAKGSEKLTAGNPGTGDHGRLRNGGSGYTAEGLRGAGSTEGRKRGLERTRGGSGGGRPAYKRPEEYLDQQALEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3L238</accession>
    <name>E3L238_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_16639</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178335">
        <property type="protein sequence ID" value="EFP90613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335032.1">
        <property type="nucleotide sequence ID" value="XM_003334984.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L238"/>
    <dbReference type="GeneID" id="10530513"/>
    <dbReference type="KEGG" id="pgr:PGTG_16639"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16639"/>
    <dbReference type="eggNOG" id="KOG4585">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018552_2_0_1"/>
    <dbReference type="InParanoid" id="E3L238"/>
    <dbReference type="OMA" id="FNTEHIT"/>
    <dbReference type="OrthoDB" id="2502344at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF85">
        <property type="entry name" value="GH03217P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="8">
        <location>
            <begin position="221"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP90613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="332" mass="38029" checksum="503D3DF977A3B360" modified="2011-01-11" version="1">MRGYSERQSLIRDMFMIIVWMEHNEVEDMVDAAILRRKPFPTDGEIAFPRNRLLQLMYDLLLSDRALFEEQLQIILRSRYLSPRPTPKTRDEFDLERLFNMPDMDFRQAARTTKAGFVEVLDIICMNPVFHCGGIRPQLPIAHQLALTLERLGSNGNAASVGRFSRNLNVSRSTVIKATQQVIEALVSLGRQYVMWPNTTRWAEISEVMKNEGFVGCVGFVDGTTIPLFQRPGYDGEVFYDRKRRYSINAQIICDCDKYITSFITGWPGTCGDSLVYKRMQLHLNPSQYFDDGQYLLADSAYEVSKTVVPAYKNPSAKIPINADFNYCLAKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3L4D7</accession>
    <name>E3L4D7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17368</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178346">
        <property type="protein sequence ID" value="EFP91412.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335831.2">
        <property type="nucleotide sequence ID" value="XM_003335783.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L4D7"/>
    <dbReference type="GeneID" id="10529326"/>
    <dbReference type="KEGG" id="pgr:PGTG_17368"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17368"/>
    <dbReference type="HOGENOM" id="CLU_070875_0_0_1"/>
    <dbReference type="InParanoid" id="E3L4D7"/>
    <dbReference type="OrthoDB" id="2501750at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91412.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="289" mass="34088" checksum="B88AAD4738BB90EE" modified="2012-04-18" version="2">MIEGPSSRKRKFAEDYLSDSDSSDSQASAPHFHDAMVSMTKPGSDESDHEWKERRIDYFEKFAKEKRPLTLRHDYHHPIDKNIVEKHIRGFSQLLELKKPDYSKRFIFHKKLPFRLVYHDDGRPGRQSEVLQAGSNLKQTDWAWKSLYETLVTSISEKHSEDYKMELFKKDIYISEQEGLLRWLDKEIFAPYNSLPILGLSRYSFGSEGIDEALLQPNQEKLINYFSQDAKKINTEPTVSELLKSYKHRPLKEPPETMKLPDYYSSEKNHESWVKLIEDPVRGYGLYYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3L546</accession>
    <name>E3L546_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_17527</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178350">
        <property type="protein sequence ID" value="EFP91671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336090.1">
        <property type="nucleotide sequence ID" value="XM_003336042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L546"/>
    <dbReference type="STRING" id="418459.E3L546"/>
    <dbReference type="GeneID" id="10531610"/>
    <dbReference type="KEGG" id="pgr:PGTG_17527"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17527"/>
    <dbReference type="HOGENOM" id="CLU_012390_7_2_1"/>
    <dbReference type="InParanoid" id="E3L546"/>
    <dbReference type="OMA" id="AWVEISE"/>
    <dbReference type="OrthoDB" id="674687at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125">
        <property type="entry name" value="F21J9.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125:SF3">
        <property type="entry name" value="NO-APICAL-MERISTEM-ASSOCIATED CARBOXY-TERMINAL DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="2">
        <location>
            <begin position="141"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP91671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="312" mass="35188" checksum="95317DEFA9102980" modified="2011-01-11" version="1">MANNNEVRDDDENIEIVTPGAPKESQDKSGEEKKKAPNYTEPEDYELCRAWVQVSEDPAVGTNQEGSTFWQRVWTVYREAVPSPKRPVSSLKKRWSNNLQPAINKFRGIVHQVEGFNESGASVEDQLNRALRLYTQDQETHFKHLRCYNLLVKSPKWNSYCRDSNQKETGKKKRARSPSSEAPQSQLASTLAPSDPVSDFEGTGSTPSMLERPIGKKKAKMINQLAAKDQTWKDEVSAAHKQIALESKRLNDIFAHDSTSIDRISGNGTTASELAIMTTDLTGLDDEQKEYFRLKRASILQSLRNQQNSSNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>E3L6A3</accession>
    <name>E3L6A3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="7">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_18168</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="7"/>
    <dbReference type="EMBL" id="DS178357">
        <property type="protein sequence ID" value="EFP92078.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336497.2">
        <property type="nucleotide sequence ID" value="XM_003336449.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L6A3"/>
    <dbReference type="STRING" id="418459.E3L6A3"/>
    <dbReference type="GeneID" id="10532035"/>
    <dbReference type="KEGG" id="pgr:PGTG_18168"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18168"/>
    <dbReference type="HOGENOM" id="CLU_011478_1_1_1"/>
    <dbReference type="InParanoid" id="E3L6A3"/>
    <dbReference type="OrthoDB" id="5409596at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="62"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="288"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP92078.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="632" mass="71218" checksum="A1FEB5A1226BDE28" modified="2012-04-18" version="2">MNRASKNPPPTIKPPPARWASLGITVLKKIAEKKDDALRREILEISRSLYGQDAKTLQIDAVVNLVRGRNTFLLAGTGYGKSRIPELYFRTLPISEKPVIIVLNPLDSLGDNQVLEKKAAKFTAINLTSLTFNKDEAELIANGFYNFVYLSPEIFLNSKMWDRVYFSDMFQRRLGLVVLDEAHMVYEWGIVEKTRGRKRSSALGRHEDRGIFRPSYGNLGGHLMTRNNMPMLLMSATCRPVAIEAIKTSLKLSDHTLRMLNGELTRPGIRFIRVTMDYSLSSCEDLLDLYARKTTTPDEKVVPTIIYSGSRHRTMKVLDVLDRARGSADSVNNSESTFARRFHSITGDLCKIDVADDFAAGKFPIVSATMALGLGQNWSRVRSVIHMGRGDPAAICQMLGRCGRDGRPGVAIMFVEKTRIGGKNRVDQILDGVEQSDDDRMDGLAITPVCLRIAFSLDTKLGYIPMSIKDQGYQNEKSREERLGFAQCRCSNCMPMEANTFIENMKLMTVDNIDRMITTDLNAYETGALVPKHPKLKIQRPWMSNKRALADPEDLRNFKRQMRETVDELHQNEHGDNTFFTPSQLFQDKKIDLIAHHADKLLTLKDIREKAHINAKEDSNKLASGGRNPSTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3L754</accession>
    <name>E3L754_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS989928">
        <property type="protein sequence ID" value="EFP94221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338640.1">
        <property type="nucleotide sequence ID" value="XM_003338592.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L754"/>
    <dbReference type="GeneID" id="10527312"/>
    <dbReference type="KEGG" id="pgr:PGTG_20121"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20121"/>
    <dbReference type="HOGENOM" id="CLU_2224499_0_0_1"/>
    <dbReference type="InParanoid" id="E3L754"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP94221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="106" mass="11670" checksum="050CBACE88CD0C7F" modified="2011-01-11" version="1">MPDEGLFMNFIAEYHRVRAEDARWTTQTASVLLKKQDGRGRDRLQSMEGLDNSDEESVQQVDILDFSTGVADSGPPCSDREAQSSGKINLLAYQVLISCTASPLSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>E3L885</accession>
    <name>E3L885_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_18644</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178372">
        <property type="protein sequence ID" value="EFP92760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337179.1">
        <property type="nucleotide sequence ID" value="XM_003337131.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L885"/>
    <dbReference type="GeneID" id="10539256"/>
    <dbReference type="KEGG" id="pgr:PGTG_18644"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18644"/>
    <dbReference type="HOGENOM" id="CLU_1504161_0_0_1"/>
    <dbReference type="InParanoid" id="E3L885"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP92760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="179" mass="19346" checksum="463E1B5D3B16DB91" modified="2011-01-11" version="1">MSASGSLCTPLLTKVHRLKRNCGRSRERRVAGASSCVRHSDPSSHIQQPTSTLISTTDILDRLRLPISSAVFDRICDASMTASSGVLGCVASCSRFLKSQVLVVLIPPPEVPEPVEAFRTESQEGANTKWSPEVVFKDQLENVDSDSWSDPFCVREVPSHGCLEPPSPNLAGCPQPSSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8Z2</accession>
    <name>E3L8Z2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">N-alpha-acetyltransferase 60</fullName>
            <ecNumber evidence="7">2.3.1.259</ecNumber>
            <ecNumber evidence="1">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_18858</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N-terminal L-methionyl-[transmembrane protein] + acetyl-CoA = N-terminal N(alpha)-acetyl-L-methionyl-[transmembrane protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:50604"/>
            <dbReference type="Rhea" id="RHEA-COMP:12745"/>
            <dbReference type="Rhea" id="RHEA-COMP:12746"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:64731"/>
            <dbReference type="ChEBI" id="CHEBI:133414"/>
            <dbReference type="EC" id="2.3.1.259"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the acetyltransferase family. NAA60 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.259" evidence="7"/>
    <dbReference type="EC" id="2.3.1.48" evidence="1"/>
    <dbReference type="EMBL" id="DS178379">
        <property type="protein sequence ID" value="EFP93017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337436.1">
        <property type="nucleotide sequence ID" value="XM_003337388.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L8Z2"/>
    <dbReference type="STRING" id="418459.E3L8Z2"/>
    <dbReference type="GeneID" id="10542993"/>
    <dbReference type="KEGG" id="pgr:PGTG_18858"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18858"/>
    <dbReference type="HOGENOM" id="CLU_051722_0_0_1"/>
    <dbReference type="InParanoid" id="E3L8Z2"/>
    <dbReference type="OMA" id="DIDAWFL"/>
    <dbReference type="OrthoDB" id="47374at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004343">
        <property type="term" value="F:glucosamine 6-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120518">
        <property type="term" value="F:protein N-terminal-methionine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061733">
        <property type="term" value="F:protein-lysine-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007059">
        <property type="term" value="P:chromosome segregation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045141">
        <property type="entry name" value="NAA60-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14744">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14744:SF15">
        <property type="entry name" value="N-ALPHA-ACETYLTRANSFERASE 60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="4" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="3" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="12">
        <location>
            <begin position="156"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="13"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="75"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="226"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="382"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="19"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="112"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="233"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="382"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="392"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="408"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00026111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00026144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP93017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="434" mass="47272" checksum="754799CD7BFDCCF4" modified="2011-01-11" version="1">MPLGCWLSLVPTTDQVPCSSSPVSPSSSSSSSTTTTSPTRSSDSYESTSSSTNSLTTPTSTTFHPSSCFIPFKKASTPHQQHKHDHHPTPNSFLKPMVLESPNSCRPRRNKPSPSSSSSSSPSLTSFHPLPLLENSHNQTSNLINNLNQKHTRSCFEVRSLQVCDIEKVRELHCATLPVTYPSSFFYNLLRSDSQEKISLVATLPSTSVQGYFEVLSSLGNHPASQLVSSSTHHGHLSHHHVTSPLSGRKPISLDVVGTITARIATSPTGPSVRILTLCVSPSYRRFGVGRLLLDSLLKQIKNRFSRLLPLNNSPNQDRIIVNLHVQATNKVAYAFYLRAGFSPVCFKPAYYSDNELKPLDPALKKLSSSSSLSSLHKSSSLDFSSPLPSSNTSTHQQTLLSEPDPPSTTGTSDSSDQTVDVDAWFLELSLDRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3L9E1</accession>
    <name>E3L9E1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_19126</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L9E1"/>
    <dbReference type="GeneID" id="10543315"/>
    <dbReference type="KEGG" id="pgr:PGTG_19126"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19126"/>
    <dbReference type="HOGENOM" id="CLU_1750563_0_0_1"/>
    <dbReference type="InParanoid" id="E3L9E1"/>
    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP93166.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="156" mass="18041" checksum="07311608C057AD26" modified="2012-04-18" version="2">MAGELEDLLGELVEWLDLNIDIHTQPLREFILILKFSRLFFNKISRVTSEDSYPILDMNTAQLLNLIDLTVSIPPKLMSFYDNIECNYAPRRNINLQKVSDLAKLFQAPLNLLSNHLSDQAANPNCPQNVHVKFIQWIRVLVIYLISIRCNNMQSL</sequence>
</entry>
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    <accession>E3L9M9</accession>
    <name>E3L9M9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178386">
        <property type="protein sequence ID" value="EFP93254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337673.1">
        <property type="nucleotide sequence ID" value="XM_003337625.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L9M9"/>
    <dbReference type="GeneID" id="10543486"/>
    <dbReference type="KEGG" id="pgr:PGTG_19200"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19200"/>
    <dbReference type="HOGENOM" id="CLU_393367_0_0_1"/>
    <dbReference type="InParanoid" id="E3L9M9"/>
    <dbReference type="OrthoDB" id="630188at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="543"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="71"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="543"/>
            <end position="558"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="701" mass="79046" checksum="78C7E80F27F95632" modified="2011-01-11" version="1">MVRSMKVFKIIVLPVSLLALLLGHFFFGETQLKDGLLHLLRNFNQQSINGHHQAAYTGSLMGWLGSQPSDSSSLFKQHSQSSLAVSPPPIKLSASDRPPQLGGRIRSGHLIAKAYRNALKDIELTVFTLVNRDPIFSTAEFKAGTLGVPGTGIRINDERRALQAWLDCTSGEGEWRWQPTNDGPEPRTMTVHKQGPLEAKCERAFYEGHQLAANRSRSPDDWDVRASLKFKWYPSSRCEALRPGKLHSSPRLTRRALCRSLSRKSTLIVGDVSQYALHDLLLDWTTTKQASCAGNLYCKIHPICSDELDPRWIGNSSQLEAGELDAHVYFKIPPQPFNQSYFVSNVTPIDHPSLAPLRSSIRYPNGSRTFLRYRRSDSLWGSSSPSHARFQPIWLHPNNGIRDINNFWYGDVRKSDLIILSRRPLGLPRRSQMSQAFIHNKHLPLSHLEYLRKHSRSQVWRPDVRLAAEEGSDGVTAAVSLIRMAIKMVMEVWLPEVLLTLKQLQKSHGRASKLIVWRGEWRIHGDCTSTSLPQKANDGFDWDRWWKDQPSRPGDGNRPHPSPPDLFSILFPNHHPPTTTTSSSSSNDPQTTQEKSKIEDLKLKNPRLVFHNLQTIFQNQLMRKLGPEFGIPFLDLESQASVWRTGLVASPSMASRPHSVAHSDCFDYCFPSPGLTLEESFLGGLLKILSVSGWTNSTGNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3LB09</accession>
    <name>E3LB09_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19718</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178406">
        <property type="protein sequence ID" value="EFP93734.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338153.2">
        <property type="nucleotide sequence ID" value="XM_003338105.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LB09"/>
    <dbReference type="GeneID" id="10535104"/>
    <dbReference type="KEGG" id="pgr:PGTG_19718"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19718"/>
    <dbReference type="HOGENOM" id="CLU_1496950_0_0_1"/>
    <dbReference type="InParanoid" id="E3LB09"/>
    <dbReference type="OrthoDB" id="10583639at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="160"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93734.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="180" mass="20204" checksum="4106C6B00C47507D" modified="2012-04-18" version="2">MISRGNPHKGNDEQQLPSAHQGPTPPTFVAIENGRVDDEQRHDKEDEEDSEYDSETLGEDDTDESDSDSEMEETSDDEQVESESNIEIEENGMIDDDDEDELSSETQMSIQHEHNHDELASDVELEENNMITDEDVDPEENNVLDDEDFDGVFTDDETISETSAASTCTESNMNAADCDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3LB84</accession>
    <name>E3LB84_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Sulphur transport domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_19820</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178410">
        <property type="protein sequence ID" value="EFP93809.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338228.2">
        <property type="nucleotide sequence ID" value="XM_003338180.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LB84"/>
    <dbReference type="GeneID" id="10535263"/>
    <dbReference type="KEGG" id="pgr:PGTG_19820"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19820"/>
    <dbReference type="eggNOG" id="ENOG502S27S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037802_1_1_1"/>
    <dbReference type="InParanoid" id="E3LB84"/>
    <dbReference type="OrthoDB" id="10254418at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046513">
        <property type="entry name" value="DUF6691"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007272">
        <property type="entry name" value="Sulf_transp_TsuA/YedE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30574">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YEDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30574:SF1">
        <property type="entry name" value="SULPHUR TRANSPORT DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20398">
        <property type="entry name" value="DUF6691"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0997">Cell inner membrane</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="76"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="131"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="168"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="209"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="283"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="336"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="363"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP93809.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="385" mass="41889" checksum="A04283CA3C8113D9" modified="2012-04-18" version="2">MMDSATLLESLKLPLIGGALLSLSSMNLMLDFSKVFGCSQALKSLQESYKLIRFHYFQNSNGQSNNNHILFNHQNLFIAAGLALSGSFLRLFMDFIERNLTHNERSIFDSSLLSNHVHHSRSDDHQVLKNVLLFTLAGLLSGAGTTLASGCTSGHMLCGMARGSKRSIVATCVFFPVAILTHRVLKPLLAQIGVFSQSDSMYQEYSESVSTIGSDQVICGAIFAAPFIAYLVLKLVVKPERWIPLRLGLLGATFGSGLTLSGMTKPSVVLGFFNFPLPTWDPSLLGVAIAGLGLNTLVYWLERPLLDKLDRLAPPCATPPTWQLPEMTNNEITTRLVLGSVLFGLGWGLAGVCPGPALVVFGAYPLSFVMWGWLAGFFAGSHFIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3LBF1</accession>
    <name>E3LBF1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_19915</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178414">
        <property type="protein sequence ID" value="EFP93876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338295.1">
        <property type="nucleotide sequence ID" value="XM_003338247.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LBF1"/>
    <dbReference type="GeneID" id="10535407"/>
    <dbReference type="KEGG" id="pgr:PGTG_19915"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19915"/>
    <dbReference type="HOGENOM" id="CLU_994446_0_0_1"/>
    <dbReference type="InParanoid" id="E3LBF1"/>
    <dbReference type="OMA" id="GEWPIAF"/>
    <dbReference type="OrthoDB" id="2496879at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="85"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="119"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="154"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="194"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="219"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP93876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="280" mass="30624" checksum="12875D78F2A11DDE" modified="2011-01-11" version="1">MNSTKDQPAYFGAPSPQRPLSMLPPKPAQLTQRQKNLVVHLKATEGKTSLVAPAYFLFSCFGKLIFGFGASMAVKGVCTNDTPSIGAGGGLCAFGFICILIVAFGFGAACHRIDKFRGILFKCISILAAILIAVGYAVNQYYVSTHHELLDIRLSFFPLFLGAALLVISISNFFAYTELVRGPTPGLPRERRPLIAMCFFGPGVGTVFGGWITGEKGEWPVAFNAAVLTLAMACLYFGICLFWSDHLLREAEWAEKSVMLEAYRLSTNEITDRLEEKEQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>E3LBU4</accession>
    <name>E3LBU4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_19766</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178428">
        <property type="protein sequence ID" value="EFP94019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338438.1">
        <property type="nucleotide sequence ID" value="XM_003338390.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LBU4"/>
    <dbReference type="GeneID" id="10535632"/>
    <dbReference type="KEGG" id="pgr:PGTG_19766"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19766"/>
    <dbReference type="HOGENOM" id="CLU_3260773_0_0_1"/>
    <dbReference type="InParanoid" id="E3LBU4"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP94019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="42" mass="4689" checksum="443D5911DC799ED7" modified="2011-01-11" version="1">MHSCPLRVYASPKPSDGFGGSTPKYWEIWGFGKKQLPLDIHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3LBV6</accession>
    <name>E3LBV6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_19949</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178429">
        <property type="protein sequence ID" value="EFP94031.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338450.2">
        <property type="nucleotide sequence ID" value="XM_003338402.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LBV6"/>
    <dbReference type="STRING" id="418459.E3LBV6"/>
    <dbReference type="GeneID" id="10535642"/>
    <dbReference type="KEGG" id="pgr:PGTG_19949"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19949"/>
    <dbReference type="HOGENOM" id="CLU_000315_2_4_1"/>
    <dbReference type="InParanoid" id="E3LBV6"/>
    <dbReference type="OrthoDB" id="448448at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050628">
        <property type="entry name" value="SNF2_RAD54_helicase_TF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626:SF22">
        <property type="entry name" value="DNA REPAIR PROTEIN RAD5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45626">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="4">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP94031.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="222" mass="25681" checksum="AB9575804E4A83AC" modified="2012-04-18" version="2">MGLDFSSIKWFRIVLDEAHYLKDPRTNRSSVILGLEAERRLCLTGTPLQNQLGDLHSLIKFIRIEPWTENSIWKNCIESPVEMCDPRGISTLQTIMNRISMRRLKTTILSLPEKIETIINLALKTPWNETYERNHKAFSDQFGKNRKGGQGWNSSSFFADIMDLRQLCNHPALTEKGIGTKKYSWNESSKIVHLVQDLKLFLASGAQFRAVIFSEFKRFLEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>H6QP47</accession>
    <name>H6QP47_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20760</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EHS63177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890732.1">
        <property type="nucleotide sequence ID" value="XM_003890683.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QP47"/>
    <dbReference type="STRING" id="418459.H6QP47"/>
    <dbReference type="GeneID" id="13542578"/>
    <dbReference type="KEGG" id="pgr:PGTG_20760"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20760"/>
    <dbReference type="eggNOG" id="ENOG502SBYH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004591_1_2_1"/>
    <dbReference type="InParanoid" id="H6QP47"/>
    <dbReference type="OrthoDB" id="2506799at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="849" mass="98450" checksum="29CE3798861ADA70" modified="2012-04-18" version="1">MDAESRKTDCLTTVSRTDPFLKHRWLTLQLPSPRLARLSQKKRLRQTIGVFQMMEGFTRVPNTHLFTCDTCGLRRMSEKRIRIHRSLESHQRKLTQARSRNHRNRQTSTIHATVTEPSTPIQEEDPRSDGMDNHNRDNEVMEDEMENPVDVANGNQEAQEVHARELRRALDLIRGMTSEDFFDLTEERAGVPEEYDRMDWLYDEMDAECMDGPGEHPNDDDEDDEANSTNDNEWYPFKNKMELVGSLLIGYTRHILSRDVFDEIRVIFRILCQIKIPAWSTIRRAQERIRKHLEMEVQFSNSVWGTPCASVGIKKILKNELANPLVAPHLEFYPQESNGCGIFKLSQSQKWLKYLPKEFRPQMCTNNDHHFYIFEPMQTFENLIAVPIFFYTINLQLYSKCYVPQITFLNQEAVRLFIPPDISFNDPNLLSIPVNQFKNDHSHLSFGSSTHSSNISTIQLFETNIGDLPCTKVNLPNPWRIKANADSPMHAEITATPNPGTSLNPCRMCNLHAPSKLDKRSLSYLLQFLQLDSDGFHSPNVPRQWEKTIENTYNLFNTYLTVNITEVKRLRLIYGVTDSINNKFIDGIRSKSPVVTKKAGELIKTDPTDMFNPFFKFQGFNGCTDTPVEILHVFLLGVVKYLIRDFMQRLKKGKNKKKLAELTGRLQSFNTSSLNIPLLKPFYLINHSKSLVGKDFKIFLQAVPFIFFPLMDQEERSLWLALCRLSSYIFQTHISHMESYLYELKQHIQIFMSHLIKFTAQWINKPKFHMLFHLPESIERFGVAPLFATEKFESFNGVLRNASTHSNKQAPGRDIANSFSNYQCLRFLLSGGIMYNKITKTISQSVNIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQR2</accession>
    <name>H6QQR2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">SMP-LTD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_21131</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="13" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EHS62810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890129.1">
        <property type="nucleotide sequence ID" value="XM_003890080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQR2"/>
    <dbReference type="FunCoup" id="H6QQR2">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="GeneID" id="13541415"/>
    <dbReference type="KEGG" id="pgr:PGTG_21131"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21131"/>
    <dbReference type="eggNOG" id="KOG2238">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005129_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQR2"/>
    <dbReference type="OrthoDB" id="26740at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033116">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035621">
        <property type="term" value="P:ER to Golgi ceramide transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd21675">
        <property type="entry name" value="SMP_TEX2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019411">
        <property type="entry name" value="MMM1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031468">
        <property type="entry name" value="SMP_LBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13466:SF19">
        <property type="entry name" value="NUCLEUS-VACUOLE JUNCTION PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13466">
        <property type="entry name" value="TEX2 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10296">
        <property type="entry name" value="MMM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51847">
        <property type="entry name" value="SMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="6" id="KW-0445">Lipid transport</keyword>
    <keyword evidence="7" id="KW-0446">Lipid-binding</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="7"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="SMP-LTD" evidence="11">
        <location>
            <begin position="271"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="41"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="182"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="478"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="821"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="884"/>
            <end position="1019"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="41"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="193"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="519"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="536"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="574"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="603"/>
            <end position="612"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="646"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="677"/>
            <end position="694"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="695"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="9">
        <location>
            <begin position="742"/>
            <end position="760"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="761"/>
            <end position="785"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="826"/>
            <end position="849"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="859"/>
            <end position="870"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="886"/>
            <end position="896"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="929"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="956"/>
            <end position="973"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="999"/>
            <end position="1009"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EHS62810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1019" mass="113011" checksum="259C554A916FCDFF" modified="2012-04-18" version="1">MFYLILFVYLLGGLTFIPIIIITIGAGLYYFSPPVKQNVQSLPSSESTPLNPQEDQDHNTKYEPDSELPRLYRAGWLTVRQTYEPSQNSESTYMGLITNSYRNFMDQRSKDSKRIKPKDRFYTVLKQNVLFLYQDEDQVDCTAAIQVSLYHVDLYPTGCPDGELFVKRKAICLQPIIQNHHHHHTNKESNNQDSERFEQPDRESNHHQPGRPAAWFLFTKNNLEKEDWYHILLASSKLNGPDSKATFQKDASLFDSEDMAKLLEGIDQQPDPIPMRWMNAMLGRLFLSSYRTHALENWIIDRIMKKLAKVQTPTFLSGIKVREVNVGSTTPFFSKPMLKELTIDGDASMEVLVSYKGEFRITIETVATINLGARFKPYVVNLVLAVVLKELSGTLLLKIKRPPTNRLWFGFTAMPHLVLDLEPVVSTRQIKWALVLKPIESRIREVVMESIVYPNLDDLVFFDTQPYAHHGGIWGDAARKEKSTNDPQDDQDSSDGSASAEDAKLKTESPSSHKKSYSEPHQQPARQTTKASLNTAREDNNLSKETMRQRAVNKNPDGSEESPEAVLPTADGSIDSSPTLSLNQPENKRSSWFSATRRQDTISTQSSQASSSRNHNKTRPVQQPSSDLEDGDAVAKLQKFLRSHPESTSSHPSTPNLDGKTIAATQLPRVSEPAEIDSTSSHPNNPSANLSEELQQTPTQPIQSSSPKPADDLTELENAVGSPLARAASTHSSEENPAPLRRLPPPPRQTLPSTFLPPPTRNISNPRSSNPTTPQAVTNQSTSTNSILNQLRTRATDKEALAASVTQARDVVIKWGAAWASKRKSPQASHSHPYNSGFNPISIDQIQNPSKDKSPSIEAHSNNNRLNSLTEPVDMDFPVSLHATDESSTVHASSSDPDLVDGDEAQKAKSYRAHRARVGMGKAKSVDLSSPQSNSHPSRSTSPVLDHPTAQLRPKPSNSKLLASPASNTTNPTADEALASVGKRNRIFSSKGQFIPAAPQSTTGLSLPSAQHDGRSNHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRR2</accession>
    <name>H6QRR2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21535</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EHS63359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889797.1">
        <property type="nucleotide sequence ID" value="XM_003889748.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRR2"/>
    <dbReference type="GeneID" id="13543042"/>
    <dbReference type="KEGG" id="pgr:PGTG_21535"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21535"/>
    <dbReference type="eggNOG" id="ENOG502SZ6Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1448386_0_0_1"/>
    <dbReference type="InParanoid" id="H6QRR2"/>
    <dbReference type="OrthoDB" id="2496918at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="187" mass="22203" checksum="E5B00DA3FB50A34A" modified="2012-04-18" version="1">MRNLNQLKAQKKRLPTNIKTGDRSRFIHEKKIKKIIKYVKESTQIATFMVIVHLSLFKEHDQEFLTPQAIRKILGFFQNLWLKLESGQSSLIEKYPWAKRNWSLLTLENPHRLHVDFIRNPEDVYRMAFNFVGYWAKKNGKGVKGYGSSDHFTNVMATMDWMVYYSNYEIISGSLKRRIKRTQQGED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRY4</accession>
    <name>H6QRY4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21552</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EHS63419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889704.1">
        <property type="nucleotide sequence ID" value="XM_003889655.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRY4"/>
    <dbReference type="GeneID" id="13542818"/>
    <dbReference type="KEGG" id="pgr:PGTG_21552"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21552"/>
    <dbReference type="HOGENOM" id="CLU_809267_0_0_1"/>
    <dbReference type="InParanoid" id="H6QRY4"/>
    <dbReference type="OrthoDB" id="2506799at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="207"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="124"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="343" mass="39939" checksum="2FFBCBEC2B29CA9D" modified="2012-04-18" version="1">MDAESRKTDCLTTVSRTDPFLKHRWLTLQLPSPRLARLSQKKRLRQTIGVFQMMEGFTRVPNTHLFTCDTCGLRRMSEKRIRIHRSLESHQRKLTQARSRNHRNRQTSTIHATVTEPSTPIQEEDPRSDGMDNHNRDNEVMEDEMENPVDVANGNQEAQEVHARELRRALDLIRGMTSEDFFDLTEERAGVPEEYDRMDWLYDEMDAECMDGPGEHPNDDDEDDEANSTNDNEWYPFKNKMELVGSLLIGYTRHILSRDVFDEIRVIFRILCQIKIPAWSTIRRAQERIRKHLEMEVQFSNSVWGTPCASVGIKKILKNELANPLVAPHLEFYPQESNGCASQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTC8</accession>
    <name>H6QTC8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21897</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178311">
        <property type="protein sequence ID" value="EHS64147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889227.1">
        <property type="nucleotide sequence ID" value="XM_003889178.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTC8"/>
    <dbReference type="GeneID" id="13541698"/>
    <dbReference type="KEGG" id="pgr:PGTG_21897"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21897"/>
    <dbReference type="eggNOG" id="ENOG502SBYH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_3015259_0_0_1"/>
    <dbReference type="InParanoid" id="H6QTC8"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="56" mass="6745" checksum="D555A45899A191CC" modified="2012-04-18" version="1">MSYMQDFFGCPDLPEKQIWSETVKHTKELWVTSQSSTQKEYERKQQLYGIKDRINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTS6</accession>
    <name>H6QTS6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6589 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_22211</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178321">
        <property type="protein sequence ID" value="EHS64340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889087.1">
        <property type="nucleotide sequence ID" value="XM_003889038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTS6"/>
    <dbReference type="GeneID" id="13541771"/>
    <dbReference type="KEGG" id="pgr:PGTG_22211"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22211"/>
    <dbReference type="HOGENOM" id="CLU_009176_0_0_1"/>
    <dbReference type="InParanoid" id="H6QTS6"/>
    <dbReference type="OrthoDB" id="2509783at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046496">
        <property type="entry name" value="DUF6589"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20231">
        <property type="entry name" value="DUF6589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6589" evidence="2">
        <location>
            <begin position="189"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="653"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="674"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS64340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="691" mass="77969" checksum="6E8B6703E3DB80C6" modified="2012-04-18" version="1">MEPEVSLNSCSGSLEDVMADYGGFVYSTDGNGPDARKKQRCHIASVICSMLSFACNRRHNGLQLENSIRFYACGVSETVNEYLHYLGLTSHRKTAVHALRSLACEAQDKIASCMAISTDVAPILCIDNLDMEERIQIATVGKQNRMFHGTWGYINIPSSDLMNSLNPNDLTLSAYHNALKNVSSLVIDPNFFLPNDDDQEHHKLVVKSQIAQVMYKYVARPSHPKGRHPLHPPEVELISHKKPEIHMLKLMNESDNSAKGIGQVMDALQHQTRLDPADFFGRLQLIDGDLGTAQIFNAIRVLRSPSEHCDHSLNNISFTLGAAHTLWNIAHTILTYHFGNSGSMDNLGVWRYLEALGIPPEKVAQKKDFTKMLQYMEQVHEATLWYCLRDVMGHHDTVIQEELPIIPTAKWNDMVDKCYDRFFSHEARRAARGFPKLSNLLTRLQDFSTVIEANRAMKRGDIGRLINVWKMWSIMTQSLPGLTHYSAYLPRLILLLTKILPPSLGKLICHSILVSPSGRPNHFVAKDFLLETHNYWLKYFYTRGGIGTQIDRLQAYFLSNIPTLRTMFDSLRLDSGAKHIQQSHKPLLTLRGLERFQQMAQPNGILTINSSSGAPAPVEIKNTYIQGLILLQEEISTKANELGRFTMHLPFDEQCESYPSDPEDQAEPKGEPNKSPSIRSGSPSQYSMDED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTT1</accession>
    <name>H6QTT1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_22215</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178321">
        <property type="protein sequence ID" value="EHS64345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889092.1">
        <property type="nucleotide sequence ID" value="XM_003889043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTT1"/>
    <dbReference type="GeneID" id="13541778"/>
    <dbReference type="KEGG" id="pgr:PGTG_22215"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22215"/>
    <dbReference type="HOGENOM" id="CLU_689139_0_0_1"/>
    <dbReference type="InParanoid" id="H6QTT1"/>
    <dbReference type="OrthoDB" id="2504815at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003606569" evidence="2">
        <location>
            <begin position="25"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="218"/>
            <end position="230"/>
        </location>
    </feature>
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            <begin position="231"/>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="329"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS64345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="398" mass="41902" checksum="055B03A72AB8FA11" modified="2012-04-18" version="1">MPSSRSLGAAILLGVMPLIQDSYSSPVGGRWESSRALGISSSVHHGMSANPIVTRKKRSHLIRPRSSQAETISQRVNQILGSSDIVPPDALPEPKEKPDAPPTGASGPEKAAPPTAEHKPQDKPTPDHEGNPFSQALGPNPPAKKDNGPGHEELIPHPIDDPSAPGPNPQPDGKPHEPHGDDHNGHEGAGGPPPKKDGPEEHPPPPPPPADSHQPVGHPEPNPLNPPHPPNGDEHPKTGPHGPEKAKTQTTEIAVIAGIRIERVDFPAPDSTPTHPPAPADANAHHGPKENPLDEKDHPPPPPVASGHDDPKSHPSEKDHPPPPAPADAHAEPKDHPPAPAAEHGHDHPAEKDHPEPKQVEIPGVIMITNDSKAGKTTEAGPRAIRITTDKLKLELLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUA3</accession>
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
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    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178334">
        <property type="protein sequence ID" value="EHS64566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUA3"/>
    <dbReference type="GeneID" id="13542372"/>
    <dbReference type="KEGG" id="pgr:PGTG_22358"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22358"/>
    <dbReference type="HOGENOM" id="CLU_2832404_0_0_1"/>
    <dbReference type="InParanoid" id="H6QUA3"/>
    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
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        <location>
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            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="40"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="66" mass="7518" checksum="8CAF449DF03AEFB5" modified="2012-04-18" version="1">MRIGVEGQQDWSRCSTANNEQANDKQADNKQANDKRADNEQADNEWAEDEQADNERANDTEKGESF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>H6QVD0</accession>
    <name>H6QVD0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22651</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QVD0"/>
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    <dbReference type="KEGG" id="pgr:PGTG_22651"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22651"/>
    <dbReference type="HOGENOM" id="CLU_2838277_0_0_1"/>
    <dbReference type="InParanoid" id="H6QVD0"/>
    <dbReference type="OrthoDB" id="2511800at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="66" mass="7328" checksum="E5DAF3E863F4DA71" modified="2012-04-18" version="1" fragment="single">MAAGHSNHTATIQTMQETSHTLRVNPPGYEINFNLLRNSFDVTSSDALTLRQLLSAADQAQIWKSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>H6QVF7</accession>
    <name>H6QVF7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DASH complex subunit DAD2</fullName>
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        <alternativeName>
            <fullName evidence="17">Outer kinetochore protein DAD2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PGTG_22737</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="20" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
            <location evidence="3">Centromere</location>
            <location evidence="3">Kinetochore</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytoskeleton</location>
            <location evidence="2">Spindle</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DASH complex DAD2 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178409">
        <property type="protein sequence ID" value="EHS62911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888509.1">
        <property type="nucleotide sequence ID" value="XM_003888460.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QVF7"/>
    <dbReference type="STRING" id="418459.H6QVF7"/>
    <dbReference type="GeneID" id="13540839"/>
    <dbReference type="KEGG" id="pgr:PGTG_22737"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22737"/>
    <dbReference type="HOGENOM" id="CLU_2347743_0_0_1"/>
    <dbReference type="InParanoid" id="H6QVF7"/>
    <dbReference type="OrthoDB" id="3230169at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042729">
        <property type="term" value="C:DASH complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990023">
        <property type="term" value="C:mitotic spindle midzone"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044732">
        <property type="term" value="C:mitotic spindle pole body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008608">
        <property type="term" value="P:attachment of spindle microtubules to kinetochore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013963">
        <property type="entry name" value="DASH_Dad2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28036">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28036:SF1">
        <property type="entry name" value="DASH COMPLEX SUBUNIT DAD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08654">
        <property type="entry name" value="DASH_Dad2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="8" id="KW-0132">Cell division</keyword>
    <keyword evidence="16" id="KW-0137">Centromere</keyword>
    <keyword evidence="6" id="KW-0158">Chromosome</keyword>
    <keyword evidence="11" id="KW-0159">Chromosome partition</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="12" id="KW-0995">Kinetochore</keyword>
    <keyword evidence="9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="10" id="KW-0498">Mitosis</keyword>
    <keyword evidence="14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00023328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00030568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EHS62911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="120" mass="13333" checksum="696BBCA7BD684FBC" modified="2012-04-18" version="1">MYTANQWPRTNQNKNKMSNNRLSPTALRLIEKQHELEALVQLNQLSHGMSVQLEQLSNQFAQGADGALITESVIQRWQNVFRAAHLAIATRANAINNAAQNPVDMLVRIPVGSAQEDDSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>H6QVK1</accession>
    <name>H6QVK1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NET domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_22770</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS989926">
        <property type="protein sequence ID" value="EHS63080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890899.1">
        <property type="nucleotide sequence ID" value="XM_003890850.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QVK1"/>
    <dbReference type="STRING" id="418459.H6QVK1"/>
    <dbReference type="GeneID" id="13541029"/>
    <dbReference type="KEGG" id="pgr:PGTG_22770"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22770"/>
    <dbReference type="HOGENOM" id="CLU_1384751_0_0_1"/>
    <dbReference type="InParanoid" id="H6QVK1"/>
    <dbReference type="OrthoDB" id="2537748at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027353">
        <property type="entry name" value="NET_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038336">
        <property type="entry name" value="NET_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17035">
        <property type="entry name" value="BET"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NET" evidence="2">
        <location>
            <begin position="31"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="NET" evidence="2">
        <location>
            <begin position="110"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS63080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="197" mass="22817" checksum="690A9500340E9C0C" modified="2012-04-18" version="1">MEQSLHSARANFVDARSVTERFPVSEYYEELDYYKKRELAQQITRATEPMQLAAVDLLRTNRPDLIADENGNIELDIDTLDERTLYKLYQLLCAPSFQSSQPARVYYQTLNFEQKRELSMRLLNATESVKTAGINLIRNMRPDLATVGNEDTVIDVEILDDYTTYHLYQIFYGPPSQSAKPERPKLAAARRNRRDGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQS8</accession>
    <name>E3JQS8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_00106</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307156.1">
        <property type="nucleotide sequence ID" value="XM_003307108.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQS8"/>
    <dbReference type="GeneID" id="10527872"/>
    <dbReference type="KEGG" id="pgr:PGTG_00106"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00106"/>
    <dbReference type="HOGENOM" id="CLU_026798_3_0_1"/>
    <dbReference type="InParanoid" id="E3JQS8"/>
    <dbReference type="OrthoDB" id="2505311at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="133"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP74150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="335" mass="38217" checksum="355550BFED24280D" modified="2011-01-11" version="1">MRNARLKERRKIKREKDSNENVTNAPPPSGIHIIRGRVQPIDLFPEITADLILRVNKYKGLVKAHEENPRKHAKPPKKQIFPRNPTNEENAAALKKVRDTFAQVNYGYTKIYDETTNQLVAMVHYLPLKTMDQQRLEDLNFLCLYLHRCKEFISRVASKNRTCGGVMWAIGWRKGYDGLEILGRYRCQKSIDKNPQGYEDLMSDSSRAGEILWDIFHGFGNVAVEKNKAHMDSYGIPSIADNNFPKNPNDKSPFGFASNLAFSSHGFYNHAHKDKGDLTELPLAFAMIVPTFKKTGKIAFASDGYNVQNGQFIFRDIKACHYKSPLEQAMPAKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3JR10</accession>
    <name>E3JR10_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00128</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307178.1">
        <property type="nucleotide sequence ID" value="XM_003307130.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JR10"/>
    <dbReference type="GeneID" id="10527369"/>
    <dbReference type="KEGG" id="pgr:PGTG_00128"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00128"/>
    <dbReference type="HOGENOM" id="CLU_894685_0_0_1"/>
    <dbReference type="InParanoid" id="E3JR10"/>
    <dbReference type="OrthoDB" id="2503819at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="288"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP74172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="311" mass="33107" checksum="07E76FD2EDA6E49D" modified="2011-01-11" version="1">MGFTKSSSKSSNAHNAFMGRNRRDIDSHHFLGPIKSRVTYFNGSMTWMKGKALVKNGEEDLFCEFEGDPNFIERRFGGNVKRPREKTMDEDHVSEEEPNGTQPEPKAQPTPETAVNNQPNKENKVAAAGDVPTPKRSRVSDPLGLGQRTLADAILQGPNEGPSRNTRSRAGSSLRGKGGHRTPLVTGTSRKGSGSTARKMSTGKAVGRKGKGKGKATDPDQLAEITAGIDGFSVFSNHQIEEDPANPNNLAVAGPSTSKMAGMDAGEWAHPVTHSAKVGVWLERTAEAGSQAPVDAPRSRAPSSIAGIVMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRC2</accession>
    <name>E3JRC2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Core Histone H2A/H2B/H3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_00542</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the histone H3 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74586.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307592.2">
        <property type="nucleotide sequence ID" value="XM_003307544.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JRC2"/>
    <dbReference type="STRING" id="418459.E3JRC2"/>
    <dbReference type="GeneID" id="10527557"/>
    <dbReference type="KEGG" id="pgr:PGTG_00542"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00542"/>
    <dbReference type="eggNOG" id="KOG1745">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_117300_0_0_1"/>
    <dbReference type="InParanoid" id="E3JRC2"/>
    <dbReference type="OrthoDB" id="842664at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11426">
        <property type="entry name" value="HISTONE H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="6">
        <location>
            <begin position="95"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="34"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="127"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP74586.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="202" mass="21753" checksum="88B1B77C1E916C74" modified="2012-04-18" version="2">MSGGKPVTGPALEPAQHYIQKGGRLKSQLSQTSASANTYPSPARVTHRSTSIPTPVNIHPDCRQPRPSLTTDSSTVFLGGIESTGSLAETLPRQAYLVSLFQDTNLAAIHAKHVTIQPKDIQLARRLRGGTESSGSRTPRCQPRPSLRTDSSAVFSGGFESSSSRTETSPRRTPRRQPRPSLTTDSSAVSAPKPSQLVWWAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRK7</accession>
    <name>E3JRK7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00411</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74455.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307461.2">
        <property type="nucleotide sequence ID" value="XM_003307413.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JRK7"/>
    <dbReference type="GeneID" id="10527799"/>
    <dbReference type="KEGG" id="pgr:PGTG_00411"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00411"/>
    <dbReference type="HOGENOM" id="CLU_003292_5_1_1"/>
    <dbReference type="InParanoid" id="E3JRK7"/>
    <dbReference type="OrthoDB" id="2506750at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052925">
        <property type="entry name" value="Phage_Integrase-like_Recomb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34605:SF3">
        <property type="entry name" value="P CELL-TYPE AGGLUTINATION PROTEIN MAP4-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34605">
        <property type="entry name" value="PHAGE_INTEGRASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP74455.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="212" mass="23426" checksum="20CDE0A811EC0BA1" modified="2012-04-18" version="2">MLKHLISLTESLLPGNSYQKAVLDLAIVAFWGMARLSELTCEREDGILRWSATLFTSDVSFHRSSLGLSAALEIRGAKTCAPGDSQTIHLQSLNHMLCPVAAVKRRLADARPCNVPLFGYNSPAGWVNLVKAKVVRTLGQVWDSHGYQGITGHSFRVGGTSLRYAIGVPVEEICALGRWTSNCYKLYLRDYSQLDLEESLSLIGNLEKAWMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRN9</accession>
    <name>E3JRN9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycoside hydrolase family 71 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00156</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74200.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307206.2">
        <property type="nucleotide sequence ID" value="XM_003307158.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JRN9"/>
    <dbReference type="STRING" id="418459.E3JRN9"/>
    <dbReference type="GeneID" id="10527819"/>
    <dbReference type="KEGG" id="pgr:PGTG_00156"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00156"/>
    <dbReference type="eggNOG" id="ENOG502RTMK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019141_0_0_1"/>
    <dbReference type="InParanoid" id="E3JRN9"/>
    <dbReference type="OrthoDB" id="3257981at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051118">
        <property type="term" value="F:glucan endo-1,3-alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11577">
        <property type="entry name" value="GH71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005197">
        <property type="entry name" value="Glyco_hydro_71"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03659">
        <property type="entry name" value="Glyco_hydro_71"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173142" description="Glycoside hydrolase family 71 protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP74200.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="508" mass="54682" checksum="3D426A38D83A5D8C" modified="2012-04-18" version="2">MLAAKWPLPFLHILLSNAVFQSLGDKQHFQLSTRAPPAAAPKLVFAHFIAGLTIDYTRDDWKNQIGLAASHGIDAFALNVGAPDNWQVDQVATAYDVASQITRTNGQPFQLFLSLDMSVIKTATEVSSWVTKFAPMKSQLLIGGKAFVSTFAGESNSFGANNASIGWQAAVKAPLAALSPPMDATFVPVWSSLNPSTAVSTNPVVDGIMTWKAWPTGNETTSTSTDLQFQADAKKNGKLYMASVSPCFYTHYSDKNFMFKSDNNLYINRWEELIAMPIQPDFIQIVSWNDYGESHYIGPIAGSPPAGTSWISGFDHQAWLDMTDYFIKWYKTGSPPAITEDKVYYNYRPHAAAAVATADPFGPPANATVSKDAVYAAVFLTSDSTAQQLRITIGAASQIFNHLKPGSISTFSAPWSGNGGDVRVELLDGSGKPLLSGTGQRPISNAIQAYNFNYASEVLMKGGGGSGPGYNSVSSQSYAKSDSSNIEILPFSRMSALAGMVALSLCSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRZ7</accession>
    <name>E3JRZ7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_01415</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP74822.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319241.2">
        <property type="nucleotide sequence ID" value="XM_003319193.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JRZ7"/>
    <dbReference type="STRING" id="418459.E3JRZ7"/>
    <dbReference type="GeneID" id="10546939"/>
    <dbReference type="KEGG" id="pgr:PGTG_01415"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01415"/>
    <dbReference type="eggNOG" id="KOG3745">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_321362_0_0_1"/>
    <dbReference type="InParanoid" id="E3JRZ7"/>
    <dbReference type="OrthoDB" id="5554140at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000145">
        <property type="term" value="C:exocyst"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009976">
        <property type="entry name" value="Sec10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048627">
        <property type="entry name" value="Sec10_HB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12100:SF1">
        <property type="entry name" value="RECYCLIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12100">
        <property type="entry name" value="SEC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07393">
        <property type="entry name" value="Sec10_HB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="2">
        <location>
            <begin position="59"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="502"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="624"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="751"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="867"/>
            <end position="909"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="516"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="624"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="656"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="886"/>
            <end position="903"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP74822.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1085" mass="119894" checksum="9554E1E3AFEC598F" modified="2012-04-18" version="2">MNSSKKQNMNDHHPLRRAAPPARPHPIAGYRAQHHHHSQRAIQAAGIDPLELQRGLHPTNAFARLDHNTLSRILSFISPIQLLTTLNRTSKTLNRLINQHDNHLLWNTKLDWLTNNPLTSHTLPSIININPDNLENLFSHPLLGPPFQQDGKEEEDLLSFDPAPSSQYPSIYPGKITNTTKARILTYKPHHHHSKATTTRKTTDYRGRFLAYYRSLLPYLDSLIEHSTDSLVFLIAGLSPLQRAELLAVLSRLLRNPALAPPRLLLNKTLHDNLNKALCAFHRALYNQFSLALNTNRLSDLNTLACVNAALPPLLLPSSGLGIPDIARCFIDKRVIFEHQPFNPLDNLRKREGLKGEQIEGVDFSAMARFFEYISETIEREGGLIQRIFPRQMNVLERLISRIIDEVIEGYVARLLTAVQSLPSPLFQITNVEVYRHLKGLVEATKKVDEDGTGRTTGRVEEMIQRMFGAHLAVYLEEELSFVKGALEQACSRWQEAGELMEGSQVGGSRGAGLTVPSTRTTDGSLGTKTKTAVLRSFKNALLLPVSVVPKTVSYSFNALSNVGVEAFHSVTHALILPSTTSIHTHGPLSTDYRHAKILRDQPSTLDSELESWLDCSPPNPSLLLPTPSSSSSSNPITHPSLINPKSTLTNRDQKQEEDELDDRDGTEPDDELSSLLSLDLVLQLITINQQAIQRLHSFLPFAPLQSLNLQIVNTVHQVFVLLLHSLTHHHIQPGFEKAFRIMSSYASPSKKEVSEKSKKKGRVGKEEGKKKNSGGGGGGGVANFFGMMDVADTIQEMVEVYSEQALGADVGGHQQTQDFLSPIGREKRAFERAIDGWVAHGMSLTIDAIVMQLDLRLRTLQLPSDFCPPPNSSSSSSSCEDDDGLSSSSATTTTTTRTTTATEKQTKPTPACTAVVDRLLAICNALQGRSDRQVLHVFYQELGLRIHALLCKHIKRTKISIEGAHQLETDIARYAQVLGSMAGESKEGADEGLHGSMDALRRVGRLFGCKTPAQLVRFIRHLGLGMVSSGSTHLQLIGSAVLPMSIDELYQFVQARDDWNRIQDVVEAELFGFGGGGGESCVLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3JT92</accession>
    <name>E3JT92_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_01964</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319790.1">
        <property type="nucleotide sequence ID" value="XM_003319742.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JT92"/>
    <dbReference type="GeneID" id="10542918"/>
    <dbReference type="KEGG" id="pgr:PGTG_01964"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01964"/>
    <dbReference type="HOGENOM" id="CLU_026798_3_0_1"/>
    <dbReference type="InParanoid" id="E3JT92"/>
    <dbReference type="OrthoDB" id="2499742at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="133"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP75371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="394" mass="44652" checksum="D8D6E3E8154E5CCC" modified="2011-01-11" version="1">MPPSLSGSSLSELEESSLSELESSLTDLESTLSTLSLQSQTENGPLINKYQQKKDARAVANERFKWCGGPKPSTKTIYKRNPTPKEIEDAYRYVHDPSKFKLYDHGHVRAFDATQNDRLIADIHFIDLNKISESTKDDIQFLCLFLHKAKQFVNPVMSTGRSCGGVMFAIGWRKSMAKLEILGIYRNQKAIDKNPEAYAEHVKDSTRAGQILWYLFHPIANVALQHNQDFMTEHCIPAFFQPAFSTKHPSSPDSFFSSNLTFTTNGFYNHPHVDDKDEPSLPFAFLLSIPTCKRTGNLAFKADGYDVKDGHFIFPECGFGIKFQPDMMCLATFRQREYIHGTLPPRGRSPFARLGMSMQIAAKVTRVADRVVHEDFEEKTDMHFGDVEFLLTKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUR9</accession>
    <name>E3JUR9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_01125</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75794.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320213.2">
        <property type="nucleotide sequence ID" value="XM_003320165.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JUR9"/>
    <dbReference type="GeneID" id="10543787"/>
    <dbReference type="KEGG" id="pgr:PGTG_01125"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01125"/>
    <dbReference type="HOGENOM" id="CLU_173493_0_0_1"/>
    <dbReference type="InParanoid" id="E3JUR9"/>
    <dbReference type="OrthoDB" id="2509477at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP75794.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="110" mass="12839" checksum="C862CDDF34A8D403" modified="2012-04-18" version="2">MLKTPQKVTATEEPAEQPISLNSIGIIVESAMTALRAEFENSLKKYSDDRCESHQRMMDKVIGLQDEVAGFDEGIKLSYRRLEGPVYRSTNNRRRRLLLLHRRARHRREQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3JVW3</accession>
    <name>E3JVW3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glutamine synthetase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_02629</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the glutamine synthetase family.</text>
    </comment>
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        <property type="protein sequence ID" value="EFP76188.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003320559.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JVW3"/>
    <dbReference type="STRING" id="418459.E3JVW3"/>
    <dbReference type="GeneID" id="10533756"/>
    <dbReference type="KEGG" id="pgr:PGTG_02629"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02629"/>
    <dbReference type="eggNOG" id="KOG0683">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_858258_0_0_1"/>
    <dbReference type="InParanoid" id="E3JVW3"/>
    <dbReference type="OrthoDB" id="3364440at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
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    </dbReference>
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        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Gln_synt_N_sf"/>
    </dbReference>
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        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43785">
        <property type="entry name" value="GAMMA-GLUTAMYLPUTRESCINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43785:SF2">
        <property type="entry name" value="TYPE-1 GLUTAMINE SYNTHETASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="126"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="273"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="283"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00021364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP76188.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="324" mass="35782" checksum="9A3457458D693020" modified="2012-04-18" version="2">MPHASEAATDAAQPKFVRLCWIDLTGRVRCRIVHRTRYEQMIQSPPESILSLPACVMGLGHQDELAPGFGPSGDLYLQPDKISYRVLTYFPTHAMVMCSLLRRREPDSHQASENHSSHFSPYPLCPRFILSSTLHSGIAESGVSYLIGFEIEVVLLKSLQPLEAVDSGPHFWSGASSFRNGAAGLACIEKVVGYLEEANITVEQWHAGSAPGQFELVLSPHAPFQSCDELIYAKELIYSVAHEMGLKATFSPKPFQAARGTRSPMHISLHKKNKQLPEGQLQSRNSSSSPPSPTARSSTTINFIESHWLAGMQHTPSSLSYYFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWA7</accession>
    <name>E3JWA7_PUCGT</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_02773</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320751.1">
        <property type="nucleotide sequence ID" value="XM_003320703.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JWA7"/>
    <dbReference type="GeneID" id="10534224"/>
    <dbReference type="KEGG" id="pgr:PGTG_02773"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02773"/>
    <dbReference type="HOGENOM" id="CLU_1960667_0_0_1"/>
    <dbReference type="InParanoid" id="E3JWA7"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP76332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="128" mass="14146" checksum="39CD2C8C79E91BB6" modified="2011-01-11" version="1">MADDKRLVPSLQKNALLSVQKEAYPYQWPITVVQRILQVEMIAKVPKLQCIAAVSSAFKSSVAEAPQSSCCVLNGLSVLETRLVGPSQIDFPGYEIVELLAEMWKGRLHRYAHSDSKQSLLGSELESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWC3</accession>
    <name>E3JWC3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02789</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76348.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320767.2">
        <property type="nucleotide sequence ID" value="XM_003320719.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JWC3"/>
    <dbReference type="GeneID" id="10534241"/>
    <dbReference type="KEGG" id="pgr:PGTG_02789"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02789"/>
    <dbReference type="HOGENOM" id="CLU_031649_1_0_1"/>
    <dbReference type="InParanoid" id="E3JWC3"/>
    <dbReference type="OrthoDB" id="2500057at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="272"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="388"/>
            <end position="406"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76348.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="455" mass="51178" checksum="25D41FF93383A39B" modified="2012-04-18" version="2">MPADSAQEFLSFVLHLPPSLNPYEATAEKMRQLTEIHAPPLWARIIRRIITIQFIILCAQCFTVLWLRKKANQLKFFRFNKLGLIHIEVLNEIVLSMFIFSALCAFDLGTEELVELRLLDFSNKFVVRTSKFLVTGNVFCCLLIGYCHWYFTLVRMFLWISGIEAAMAFTSLRARARAGSSIRQSLFVRCAANGSLLAILTIPSAVILWLSVRAALNLRAIEIATKKIISGLLKAAPTYQPHTYQLMKLLEILKPAEGFAIHMQHLLYDTRVIVLMFLVFHLVVTTLHLPTSVLALRGIRNHISPARAPKKGAQEDPALGYQTSMMNRMNDGPAQERAALESVRKRLVIYSGLVYLDTVLYCPLLAYMYSYKGAEFLDNATWMVVEQIGTHAHTAIVGNIILAVLIQNTMYTTGNTVTGKASEHHIVVEQGACKVGFVCQPTKSEEFDLQVRSVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3JY80</accession>
    <name>E3JY80_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_02466</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP77005.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321424.2">
        <property type="nucleotide sequence ID" value="XM_003321376.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JY80"/>
    <dbReference type="GeneID" id="10529272"/>
    <dbReference type="KEGG" id="pgr:PGTG_02466"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02466"/>
    <dbReference type="HOGENOM" id="CLU_1791534_0_0_1"/>
    <dbReference type="InParanoid" id="E3JY80"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP77005.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="176" mass="18611" checksum="F72E90AE9251D3F2" modified="2012-04-18" version="2">MVVQFVGTQGNREFLLMPMGVGNGEGAPQANVALQEAVQDLPGEKRTNVVEEGASKSQANAMIGASNFSPEEVIQILGRPIKVEKGVPAPKQLITGVKGGSGQDIVTARSPVTSEESDWGPMIDNLLGQKACLEADDVTEEESVDEWWDAVEQESQVSVECGISASKNNSDLGPMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3JYF7</accession>
    <name>E3JYF7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_03038</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77082.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321501.2">
        <property type="nucleotide sequence ID" value="XM_003321453.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JYF7"/>
    <dbReference type="STRING" id="418459.E3JYF7"/>
    <dbReference type="GeneID" id="10529430"/>
    <dbReference type="KEGG" id="pgr:PGTG_03038"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03038"/>
    <dbReference type="HOGENOM" id="CLU_654052_0_0_1"/>
    <dbReference type="InParanoid" id="E3JYF7"/>
    <dbReference type="OrthoDB" id="2503998at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="111"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="371"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="403"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP77082.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="420" mass="47409" checksum="7C0BA1EDF5D5EC67" modified="2012-04-18" version="2">MQHNNQASSSARERHPNSQIPLPQSNYAKHESGATRDSRRRKLKKQKVRPEKRKAGNLEYTNERNIAAHVSRGNKDRPIHKFKNSFVCYSNNARTKAVAVVKFYPFEGMDPERKAHYERLSTNLIAHTRFQNANKSNENTLGGTMYSLGWRKGYEGKSHLGITGIASKVAKDQEGWIKLFMETPWINDFLAQRFRDISLGMCREVKEQHDQQKAPSLTPFYMADPESFCGHLSFTFNNFYNKAHKDEDGSPFRFAMWIPINKQTGKLVEENLQVEGGEFVFPEDGCAIDFSGFNGVVECVWKASEHPHHTLPSSTAIGSSDDRLGLSCLLPDSAERAIERMMAVEDGEKLDWTIQDLGHLHNDSLKYDSKGKPFETNDGVDLQASPPSACVLDEDSSTEPDKSLSSQLKVATPTQLHRST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3JYP0</accession>
    <name>E3JYP0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCZ1/INTU/HSP4 first Longin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03121</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77165.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321584.2">
        <property type="nucleotide sequence ID" value="XM_003321536.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JYP0"/>
    <dbReference type="STRING" id="418459.E3JYP0"/>
    <dbReference type="GeneID" id="10537376"/>
    <dbReference type="KEGG" id="pgr:PGTG_03121"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03121"/>
    <dbReference type="eggNOG" id="KOG2622">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018258_0_0_1"/>
    <dbReference type="InParanoid" id="E3JYP0"/>
    <dbReference type="OrthoDB" id="240546at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="344"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="426"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="558"/>
            <end position="588"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="684"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="291"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="379"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="435"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="454"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="473"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="558"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="578"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="684"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="707"/>
            <end position="716"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77165.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="761" mass="84043" checksum="AB590DA6ADE74085" modified="2012-04-18" version="2">MTSKLSDTFNILSQQQGPSSNYEPSESVLLNSLSSAAQEYHLLHGSMSLLLPNKLHALRERLEKFWSVWLWRWNVGKTGCGAVDFNELFGGLQSPFLESPKMPRAPVDLFARFVNDVCPAFSVLPILIHDRKVLHIPCPIGMIKQDDIQTLVRYLLSLLEGSEAKRVQVRPVAKVKVVEPEQNVIMKRLSGFDFGTLASLQPKLASTTNLAIQPASAWAHKAFKWASTSLVLPSNLFENIENESESLDQLLRADIGLPPRTSPPQLVRIKSSLSDPKVVAQSVPSPTRLGSQRSPSSTVSSRTKLGYPHNLLAIYPQAHSSHSRGIHYPDSLLNLYGSIAVSDSPEEETGIHSLPTVPQDPARSSEPPTKADSTGEKVTPSSLGQSKHNPRSPSFNVEHALADAMSERSIGSIAALEFVRSQSFTEFPRSDSSDPSRPSSELPPSDTLILEPDQPNSVSPDTDTPVNAPVNRGSATIRSPSTSDTTALKQLQVYVNTGKSTSSVSWLQIDGWTLAWLAQPSDPLDSSSQLDEGSDVVEKEPLLKQARYVLDSLIQSKWPEESEPERPEQQRTAERVTRGTQMTSGKVSRQRFMIKDGHSSTGLFIQQAGPQSGWLSKNEIWPGKTIPPSEVEVHLGLLRLLDDSLFGRVGGSSELVSHSGISLFEEGFLRTKSGQWIISKRIPHHHHHHLQRRQKQKTEGAEDAQEQPHSIPTSQVKTDTFDRFIEAFLVLPAGFGSLIEADNEMRILERQSEMLNMTIFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZW6</accession>
    <name>E3JZW6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03547</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322010.1">
        <property type="nucleotide sequence ID" value="XM_003321962.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZW6"/>
    <dbReference type="GeneID" id="10538799"/>
    <dbReference type="KEGG" id="pgr:PGTG_03547"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03547"/>
    <dbReference type="HOGENOM" id="CLU_2211276_0_0_1"/>
    <dbReference type="InParanoid" id="E3JZW6"/>
    <dbReference type="OrthoDB" id="2511075at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173411" evidence="1">
        <location>
            <begin position="23"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="107" mass="11773" checksum="2903EFD19F132650" modified="2011-01-11" version="1">MCLIAAIIHLTLVNLLMTLCNALKMHAYGSCPGHVSLYVKDSKCECAINFIQYIETYCSTCHKSNNELATICQVCNNQNIDRSTGVYSATAAIVSTHPAHPEKTWHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3K0Y4</accession>
    <name>E3K0Y4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_03915</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP77959.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322378.2">
        <property type="nucleotide sequence ID" value="XM_003322330.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K0Y4"/>
    <dbReference type="STRING" id="418459.E3K0Y4"/>
    <dbReference type="GeneID" id="10541107"/>
    <dbReference type="KEGG" id="pgr:PGTG_03915"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03915"/>
    <dbReference type="eggNOG" id="ENOG502S617">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056787_0_0_1"/>
    <dbReference type="InParanoid" id="E3K0Y4"/>
    <dbReference type="OrthoDB" id="2150628at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051667">
        <property type="entry name" value="Archaeal_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37096:SF1">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37096">
        <property type="entry name" value="YALI0E33429P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172918" description="AAA+ ATPase domain-containing protein" evidence="1">
        <location>
            <begin position="29"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="2">
        <location>
            <begin position="44"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP77959.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="324" mass="36385" checksum="F292F462F0B25BFE" modified="2012-04-18" version="2">MAGWKGFLPAGKVLPVAIFAVATPCARSSKTQQVEHLTTQLKAPPQFTVLLGPPSSGKTALARHVATQNRLNNTPEFHPLIIDLREVDTSEKGSFLNAFIDDGLIRTAVRNTFWGFMSNVAEVAALGVSANLRDSHPSPQKAATVFRELGSRLKPFSLSHHGERPPVLIIDEANAFKQIDDKATGAFLSFAVRITKQESKMHVIFTSSDSFFENWLKKRINPTHFNTLVVGDLPREEAYKYLLHLVETHPYLSSQNKNRLKSVNFDVPFRITGGRMFFIKQYVEQVHMSGYFEDPMDFEPTLTSCATLEDDFRGKAKTYKKKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3K240</accession>
    <name>E3K240_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Carnitine O-acetyltransferase, mitochondrial</fullName>
            <ecNumber evidence="16">2.3.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PGTG_04365</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Carnitine acetylase is specific for short chain fatty acids. Carnitine acetylase seems to affect the flux through the pyruvate dehydrogenase complex. It may be involved as well in the transport of acetyl-CoA into mitochondria.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(R)-carnitine + acetyl-CoA = O-acetyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:21136"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57589"/>
            <dbReference type="EC" id="2.3.1.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.7" evidence="16"/>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78409.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322828.2">
        <property type="nucleotide sequence ID" value="XM_003322780.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K240"/>
    <dbReference type="STRING" id="418459.E3K240"/>
    <dbReference type="GeneID" id="10536007"/>
    <dbReference type="KEGG" id="pgr:PGTG_04365"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04365"/>
    <dbReference type="eggNOG" id="KOG3717">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013513_5_1_1"/>
    <dbReference type="InParanoid" id="E3K240"/>
    <dbReference type="OrthoDB" id="240216at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004092">
        <property type="term" value="F:carnitine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009437">
        <property type="term" value="P:carnitine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.70:FF:000007">
        <property type="entry name" value="Carnitine O-acetyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF103">
        <property type="entry name" value="CARNITINE O-ACETYL-TRANSFERASE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00439">
        <property type="entry name" value="ACYLTRANSF_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="19">
        <location>
            <begin position="29"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="18">
        <location>
            <position position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00053195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00066910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00073438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR600542-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="EFP78409.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="622" mass="69998" checksum="8077CB7973C1BD4A" modified="2012-04-18" version="2">MRSQLSHFGMPSHRSKAITYQHQDSLPHLPVPELQSTAHKYFRSILPLVSPQEPNTPSASDLTPTPSYEHTKACIEDFLKSPLVKELQARLKKRAEDEGHDNWLSDLYNQWAYMGAREPLIPFSSYYIAHKPDHRWTTAAKRASGLVRAILEFRNLLEAEQLEPELARSVPLCMAGYRWLFNSCRYPSKPSDTAKKFDPQVNNHLVVIRKGQFFEFSVTKPDGSWLSTTELELQFEKVIKLAGPTETPFPVGVLTTQDRDAWADTRVELCNTNCHNIKSLERIESSILCVALDDTSPITRDELGTSLWSGGGKNRFFDKQQLIVCENGESGFNAEHSCMDGTPVARMNDWIVDVLAKEKIDLGTSSQSNLPTPSPIEFVLSDTNKQNILKAITKFESLMYPHTLEVLDYAGYGSRVIKSQFKSSPDAVAQMIFQLGYYKLFGRVPVTWEPSHTRKFKLGRTEVIRSCSIEALEWCKAMENDGADWNGRLERFKIAVKAHLSYSQQASEGQAVDRHLLGLRLSLNPGEEIPALFRDPVYKESTSWTLATSPMPSENFNGFGYGAVVPDGFGLGYAVNKESIRFTVTTPTENGARLKHCLQEAADDILKMMKFEKGQSSISAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3K414</accession>
    <name>E3K414_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04772</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323235.1">
        <property type="nucleotide sequence ID" value="XM_003323187.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K414"/>
    <dbReference type="GeneID" id="10536836"/>
    <dbReference type="KEGG" id="pgr:PGTG_04772"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04772"/>
    <dbReference type="HOGENOM" id="CLU_344556_0_0_1"/>
    <dbReference type="InParanoid" id="E3K414"/>
    <dbReference type="OMA" id="QDANTWE"/>
    <dbReference type="OrthoDB" id="2510388at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="577"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="143"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="227"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="299"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="357"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="439"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="583"/>
            <end position="595"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="870" mass="94398" checksum="7CE8350632FE393D" modified="2011-01-11" version="1">MAELLEAHIVQRMTELMELDGMAGTHIRVLSNLRSFYVSKYITRFRAVHPQIPLPNWIVTLASPGDLRPSDTEVRVTNMRAELDGETNRPPSPQQGNVISNEQEAMDDDPPRNDDNPLLPQAPEMTDIRPPQEDVVPVQGPDTTKDAIHHDKEMDLTSSDAEGEVDPEISFDVGNGHVAQSGKTKGATDGSNTEGTNMDKPLFLPESNPPSEDDSPLWGSNPKVGKSNRSCSPLSSPPSASLSEPDNPQPSPPAPNNPPLGLGNVVTLGQKRPITRSLTGDATRKHRPRLDSPSLASSDSDDSSSESEEEESQGGKSDLTHPPPLPTFGHIRQPRDQTPADSDEVDTDGPNSPKPLPKTAVVTTPNPTPATTPLVPAPTSSNTVNAGAPNHRPAGTGTTLGGGGPDLTARTKSTGPTAPTRGMTPTGPSRGPNSPPLNSPTTNSGGTPGTAAKHPTPGTLGTDPTSRQVPLREPSPDLFVRLPVGDLKCLEYWEQHKESRVDPVKTARFIEKTQCIIGLLPGLLGYKSPARPAIESPMLKNVNLVMDRCKRDNVLLGHDRDCFIGVEFVNRACLGPRKKPGQSSSMSLRSAASVSPEPEHPLFPIINSTADPVRGAWKRLNDTIHDIIMLLACQRANMVMDDLKDVDGHSDAALAKVMEFFHDITTRGADDPKPSRLRVPDGSGDLSSTGMGHIYTNIALAVTALINLHFDKKSQEKPVILKKDTHEKMNRTSTKAIRSLSHLLVFGPVSMFSCPTDRKYSAQWQSCMLVEWGAMVVTERAQNAANFPEPPWSNFGNTRLHIINALSRMGFGNNEPMDWKHVMKVFEEDFSTKKLAEVLWEDIVLMTDYDVQKGATVFWADGPPYQVQQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K5D6</accession>
    <name>E3K5D6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06053</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79732.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324151.2">
        <property type="nucleotide sequence ID" value="XM_003324103.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K5D6"/>
    <dbReference type="GeneID" id="10533094"/>
    <dbReference type="KEGG" id="pgr:PGTG_06053"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06053"/>
    <dbReference type="HOGENOM" id="CLU_009635_1_1_1"/>
    <dbReference type="InParanoid" id="E3K5D6"/>
    <dbReference type="OrthoDB" id="10332044at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79732.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="122" mass="13639" checksum="72E3C4D93957E18B" modified="2012-04-18" version="2">MIIRCFCHKIALTVNAGLANLGMIAPPPSKVKESILQMFLYSNVMEPIAEVEEDNNDQAGSLAHETNPSIDDNEISEPEHDEEELARLGNQQDEEEEDTNQVEYQAANCNESISLDHLTRKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3K5Q6</accession>
    <name>E3K5Q6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_06130</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324228.1">
        <property type="nucleotide sequence ID" value="XM_003324180.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K5Q6"/>
    <dbReference type="STRING" id="418459.E3K5Q6"/>
    <dbReference type="GeneID" id="10533334"/>
    <dbReference type="KEGG" id="pgr:PGTG_06130"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06130"/>
    <dbReference type="HOGENOM" id="CLU_063508_0_0_1"/>
    <dbReference type="InParanoid" id="E3K5Q6"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051834">
        <property type="entry name" value="RING_finger_E3_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931:SF3">
        <property type="entry name" value="RING ZINC FINGER-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931">
        <property type="entry name" value="SI:CH211-59O9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="266"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP79809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="330" mass="36645" checksum="C2F9E4713E3A9EB3" modified="2011-01-11" version="1">MDQQSTTTNPIPIPIAGEEGPGGPNAAHEVAPSQLATDSYHVSGIVQCVQFASDITECPGAGALAEVEEVEFTIDIPPSHADTCPDSCSPSMCHLDWPACEDPQCACSGADSDLQDFYMLAECHQRYREMQGPHEDDFPSTYPIGPALETLPPHNQTLIKDYFTRLSAAYAELWDEKPSRVPMTEEELSELFGPQGSLDTLENIGDFHRERIESFFQSFERVFKRKNASLGPQLPTLPQLVDSLTKPAGYLKLESNEPDRQAAPSCSVCLDAYLPDEPVVTLPCDPSHHFHLHCAKGWLEAQEDCPVCRIPLDIFKEEDYSFLDDELEWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>E3K779</accession>
    <name>E3K779_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05041</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP79816.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="E3K779"/>
    <dbReference type="STRING" id="418459.E3K779"/>
    <dbReference type="GeneID" id="10533349"/>
    <dbReference type="KEGG" id="pgr:PGTG_05041"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05041"/>
    <dbReference type="HOGENOM" id="CLU_1008790_0_0_1"/>
    <dbReference type="InParanoid" id="E3K779"/>
    <dbReference type="OrthoDB" id="3352408at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="entry name" value="Calcium-transporting ATPase, cytoplasmic domain N"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR023299">
        <property type="entry name" value="ATPase_P-typ_cyto_dom_N"/>
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    <dbReference type="SUPFAM" id="SSF81660">
        <property type="entry name" value="Metal cation-transporting ATPase, ATP-binding domain N"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79816.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="276" mass="29983" checksum="1168CA8E7BD75773" modified="2012-04-18" version="2">MPPGGIPPGELVHRPARLFSSTHGPATRLSDLISSCHISYQQRGTTTSSNRPRPDGSLKLLPGGRDSSRRAGVHTSLSGGVPPGEPDFKPARQEESLPASWYKDQLVRRDSFRRAGARRGFDRRSLSASLTIPTLPRLIAQLKLLTTINLRLDFLVDQARIQQNLTPGLIQLFNESIAINSTAFEAKTGGGQLEFIGSKTETALSQRKAMGVVVRLPESGRYRLFLKGASEVLTKLTSHYVCVRGPSSEGQPINPELEDVSPTSCASEYPQVDADA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3K7A6</accession>
    <name>E3K7A6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_06211</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80255.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003324626.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K7A6"/>
    <dbReference type="FunCoup" id="E3K7A6">
        <property type="interactions" value="753"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K7A6"/>
    <dbReference type="GeneID" id="10534598"/>
    <dbReference type="KEGG" id="pgr:PGTG_06211"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06211"/>
    <dbReference type="eggNOG" id="KOG1672">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072378_0_1_1"/>
    <dbReference type="InParanoid" id="E3K7A6"/>
    <dbReference type="OrthoDB" id="10257948at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02989">
        <property type="entry name" value="Phd_like_TxnDC9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21148">
        <property type="entry name" value="THIOREDOXIN DOMAIN-CONTAINING PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="2">
        <location>
            <begin position="84"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP80255.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="221" mass="25301" checksum="BF7A4E74486A8E99" modified="2012-04-18" version="2">MATSTTGKIGSITEQRRNESEDDHEEVEDDEKLLAQLDDDFELNGIRERRLEELRKEITKNQQMSEDNHGRYVEIKLEKKLIQITAKAKTSVVHFFHPDFERCKTMDKKLEELASKYFSTRFLKVNVANVPWLVEKLQIKVLPCVVGFLDGISKERIVGFEGITGESSKEINAIALELRLKQADLIKDEVNIGSAPAHHRREIFGSAIRQDSDGDSSDWDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3K7E9</accession>
    <name>E3K7E9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_06229</name>
    </gene>
    <organism evidence="4 5">
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        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="XP_003324692.1">
        <property type="nucleotide sequence ID" value="XM_003324644.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K7E9"/>
    <dbReference type="GeneID" id="10539279"/>
    <dbReference type="KEGG" id="pgr:PGTG_06229"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06229"/>
    <dbReference type="HOGENOM" id="CLU_078863_0_0_1"/>
    <dbReference type="InParanoid" id="E3K7E9"/>
    <dbReference type="OMA" id="QCKISNC"/>
    <dbReference type="OrthoDB" id="2505513at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018828">
        <property type="entry name" value="RRG7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28133">
        <property type="entry name" value="REQUIRED FOR RESPIRATORY GROWTH PROTEIN 7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28133:SF1">
        <property type="entry name" value="REQUIRED FOR RESPIRATORY GROWTH PROTEIN 7, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10356">
        <property type="entry name" value="RRG7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="38"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP80273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="209" mass="23300" checksum="FF6101B0AE0F9DE6" modified="2011-01-11" version="1">MEEVLLMQDLVHVGKRGSQDGGIDIRGWLYSNHRTGIQAQPQDRPVEADKPNQAQQSEDADHGKQERRFIRAIAQCKCARVELKIVRELEGLVATSSTYNNPLDSKPTIGLLFASRGFTSASLTRAERSPFPLVLVSLDLPPTFLSLSDVESEGVDQMNHPCKISHCYPNPVAVRLIHPFEFIRVSKLTSDGLPAEQSWILVNRTINES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3K7L2</accession>
    <name>E3K7L2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Lysophospholipase</fullName>
            <ecNumber evidence="2 9">3.1.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_06268</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the lysophospholipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="2 9"/>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80312.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324731.2">
        <property type="nucleotide sequence ID" value="XM_003324683.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K7L2"/>
    <dbReference type="FunCoup" id="E3K7L2">
        <property type="interactions" value="216"/>
    </dbReference>
    <dbReference type="GeneID" id="10539320"/>
    <dbReference type="KEGG" id="pgr:PGTG_06268"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06268"/>
    <dbReference type="eggNOG" id="KOG1325">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014602_1_0_1"/>
    <dbReference type="InParanoid" id="E3K7L2"/>
    <dbReference type="OrthoDB" id="4084751at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000030">
        <property type="entry name" value="Lysophospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF33">
        <property type="entry name" value="LYSOPHOSPHOLIPASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8 9" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6 8 9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005127845" description="Lysophospholipase" evidence="9">
        <location>
            <begin position="24"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="domain" description="PLA2c" evidence="10">
        <location>
            <begin position="52"/>
            <end position="573"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP80312.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="573" mass="64246" checksum="EEF02A557368096C" modified="2012-04-18" version="2">MNLTHHVIYLWCLFHFTVTPSTSYNLNRQHLTRRDAAISNSPSGNYAPVVDKCPTDFFTRQPNDAEAKLSKSESDYINTRAGHSIPSWEEYLNRVQLKDFDVNSFLHKAKQNGGKAGETLPNLGFSLSGGGARALCVTASILDAFDSRNEKANEGQVGGILQLANYAAGVSGSSWLLGSWATSNFPRISTLDWRLDEENDLWDWNVAKHFLKVWKIVKKKKKAGFHVSIDPQTGKAVLWSSIRQTSGYKERSAPYVMAIATSRPEKKTAFTPETPIYEFTPEEFGVWHPYLNVSIPMEYLGSPPETYKSPSTSSCVKGFDNAGFVMGMSSNIYSAADSPDTSDLPSFIRMLDKFVDDDDWEGKLPNTFQGLGKNGHFQDDKRDTLLMADCALTMENVPIFPFLQPSRKIDVIIAVDSSADGVKPSDPIQYGYPNGTALYTIYTKTLQPHFSGYRMPKIPNPYDGSFTKAGYHQRPTFFGCDSKPKTPLIIYLPNYYMIGKTNVPTKETTYSKERMDEFFENGFAIATQNTGFKADTEWPACLACALIDHQIQRNSQARTKQCQRCFDSYCARV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBD3</accession>
    <name>E3KBD3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_07895</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326065.1">
        <property type="nucleotide sequence ID" value="XM_003326017.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KBD3"/>
    <dbReference type="GeneID" id="10533190"/>
    <dbReference type="KEGG" id="pgr:PGTG_07895"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07895"/>
    <dbReference type="HOGENOM" id="CLU_523917_0_0_1"/>
    <dbReference type="InParanoid" id="E3KBD3"/>
    <dbReference type="OMA" id="QIETHDV"/>
    <dbReference type="OrthoDB" id="10366534at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173715" evidence="2">
        <location>
            <begin position="25"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="170"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="268"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="322"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="340"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="387"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="416"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP81646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="446" mass="45283" checksum="0C70E12FBB110D09" modified="2011-01-11" version="1">MVSFKLVQFISIVVFASTCMDTQAGLVTRAAPAPKANDVSLDPTDVPGGKNANINSKNPKLPASAVPVGSPKNPKSATPSEGKTDPKNPKSAAGMPGGNSKTSEPLKNHSPEEVKALMDKLKLKPNSTGAPGAGSGTPDQNPKSKTKAGGGSGTPPAGPGKTSKPNGMTRRAEAATKSAEDKKASFMEKLKAKSSASGRPAGTPPTELTKRADAPGGGSDDKKAAFLEKLKAKKAGGGGLSKGGEGGAGAMGAKSPMSKRADSPSGAPEEKKGGGLLDKLKSKLSAADHSKGDVSHSKGDAVHSNGDAGHAKGDAKPSNGDAGHDTGAVEKTAKPLGISKRAEHLTWKREDAAPASPEKNDKASAEPETKKPKESKPAKEHKHKAPKEKANPASKDSKPKEQKKPATPEAEGTSEAPKEKANPTSKDSKPKEQKKPAAPEAEGTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBL5</accession>
    <name>E3KBL5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_07946</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Secondary metabolite biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class I-like SAM-binding methyltransferase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326116.1">
        <property type="nucleotide sequence ID" value="XM_003326068.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KBL5"/>
    <dbReference type="GeneID" id="10533404"/>
    <dbReference type="KEGG" id="pgr:PGTG_07946"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07946"/>
    <dbReference type="eggNOG" id="ENOG502S21N">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051542_1_1_1"/>
    <dbReference type="InParanoid" id="E3KBL5"/>
    <dbReference type="OMA" id="FIGRWKF"/>
    <dbReference type="OrthoDB" id="2497308at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051654">
        <property type="entry name" value="Meroterpenoid_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35897">
        <property type="entry name" value="METHYLTRANSFERASE AUSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35897:SF1">
        <property type="entry name" value="METHYLTRANSFERASE AUSD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00038314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP81697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="315" mass="35725" checksum="DBB2AC4561F481B4" modified="2011-01-11" version="1">MAALTEASSLPSAPANPAGTPGGMTAENVPKPFEGAEAIYPLDDARYVEDLKFFQKSTGIEDPLALKKHIFKIRDEAYKTFPYPCIWGFDFLKRKTLTHPFYPQVKASKQSDKQQKVFMELGSFVGVDLRQVVQDGWSPNNVLGVDIRREWTDLGHQLFNDGDRGLPFFLGDIFQSEVLDVAPAEAARPVLDLKNLKDLNPLKERVMYICGHQVFHLFPEPVQRDLLERCWRLLSPEPGAAIFGTQVGTPEGQTMKGSQAYMHSPKTMEKLVKEVLGENVKLTMEVEKYDAEHNYHKFPLGLEGMFWLHWSVERI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBS1</accession>
    <name>E3KBS1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MARVEL domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07783</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81534.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325953.2">
        <property type="nucleotide sequence ID" value="XM_003325905.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KBS1"/>
    <dbReference type="STRING" id="418459.E3KBS1"/>
    <dbReference type="GeneID" id="10532914"/>
    <dbReference type="KEGG" id="pgr:PGTG_07783"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07783"/>
    <dbReference type="eggNOG" id="KOG1775">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1147666_0_0_1"/>
    <dbReference type="InParanoid" id="E3KBS1"/>
    <dbReference type="OrthoDB" id="3365245at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035838">
        <property type="term" value="C:growing cell tip"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051380">
        <property type="entry name" value="pH-response_reg_palI/RIM9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009571">
        <property type="entry name" value="SUR7/Rim9-like_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28013:SF4">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28013">
        <property type="entry name" value="PROTEIN DCV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06687">
        <property type="entry name" value="SUR7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="144"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81534.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="242" mass="26394" checksum="E7FE5D3F0295AF16" modified="2012-04-18" version="2">MPIFGPNTLGTLVILLATIALIVVLASAPHHPNLYFLAATVNSKASNLNQAGIIKLGVFGYCISTEQGTEVCPPPSIGYALDPKVMFGLPELPEMIKLSDNIIRNMTKVLVLHVLATILAGLAFISGLVSHIEEFSKTCWTSCFASMSASVTLLVTIFDLIFFSIIKARINAMKSSDQSISAQFGNALGLTIVAWILLAFSGCFFCAGRCLCSFRRHKRSNEHHRLEPFTSEKSGRQYPPYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCE4</accession>
    <name>E3KCE4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EFP81892.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326311.2">
        <property type="nucleotide sequence ID" value="XM_003326263.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCE4"/>
    <dbReference type="GeneID" id="10533929"/>
    <dbReference type="KEGG" id="pgr:PGTG_08141"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08141"/>
    <dbReference type="HOGENOM" id="CLU_2027882_0_0_1"/>
    <dbReference type="InParanoid" id="E3KCE4"/>
    <dbReference type="OrthoDB" id="10383737at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="73"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="103"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81892.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="122" mass="13288" checksum="14DE5E1DDDF6AC88" modified="2012-04-18" version="2">MKQHRQGRASKSVLEIDCACESYVDWPIPVTVASKVSLGQLRISLVAGEARLRSALRFVSPFPNQGHALSISMTQQSSSLDNQNSIILHNDHPPTQETHDTVSESPSGHSQSVEEMQGKLPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCI4</accession>
    <name>E3KCI4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_08181</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EFP81932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326351.1">
        <property type="nucleotide sequence ID" value="XM_003326303.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCI4"/>
    <dbReference type="GeneID" id="10534132"/>
    <dbReference type="KEGG" id="pgr:PGTG_08181"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08181"/>
    <dbReference type="HOGENOM" id="CLU_2172286_0_0_1"/>
    <dbReference type="InParanoid" id="E3KCI4"/>
    <dbReference type="OrthoDB" id="10268694at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP81932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="110" mass="12143" checksum="0CCEF5E36357CB34" modified="2011-01-11" version="1">MIQAKGDKQQHYDSFPCSDPAKPHGVCTIESAHNSWTLAAPSVVKPYLFVCKEENWPIWPSGNALPGQRVRACCTEAAYEPVVERYGKQTYKAHYNIVCKPVDGAGPLPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3KEY9</accession>
    <name>E3KEY9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_09809</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178284">
        <property type="protein sequence ID" value="EFP82841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327260.1">
        <property type="nucleotide sequence ID" value="XM_003327212.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KEY9"/>
    <dbReference type="STRING" id="418459.E3KEY9"/>
    <dbReference type="GeneID" id="10544550"/>
    <dbReference type="KEGG" id="pgr:PGTG_09809"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09809"/>
    <dbReference type="HOGENOM" id="CLU_1225300_0_0_1"/>
    <dbReference type="InParanoid" id="E3KEY9"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="196"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="131"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP82841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="226" mass="26314" checksum="481D258950C7DED1" modified="2011-01-11" version="1">MNKKYDLDQQQKLILFPIQWMSHEYNFVDKKPCMSIEEIEKEDVKVDYNNATGSFFNFFAFKEDLYELHSHLLELHSKAFEQVFPLANLDPTCCLSSPCIIIYFSFESLYAGVIEANYNSFAVSEDEEHEEDNKYKEASEEKEDSKDKEDSKDKQDNKGDNEYEADNNLNQVADLESDSNNKDVPRKKTFKVPAHPGIHTLFSLILFGVRILFYLFLFGVEFSISC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KFB1</accession>
    <name>E3KFB1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_09906</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178284">
        <property type="protein sequence ID" value="EFP82938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327357.1">
        <property type="nucleotide sequence ID" value="XM_003327309.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KFB1"/>
    <dbReference type="GeneID" id="10544649"/>
    <dbReference type="KEGG" id="pgr:PGTG_09906"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09906"/>
    <dbReference type="HOGENOM" id="CLU_046892_0_0_1"/>
    <dbReference type="InParanoid" id="E3KFB1"/>
    <dbReference type="OrthoDB" id="2495597at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173812" evidence="2">
        <location>
            <begin position="20"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="179"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP82938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="542" mass="61796" checksum="5D4C05532645D255" modified="2011-01-11" version="1">MRTPFIVSFLILLLIGSASLTLPTSPGEKTIIIANDGNELPDLNGPSPGVELSPGPAFLWGPNLYNTKVPKSSSIPSWEQMRPAASRIPGFLQETISLGLNPSGSRKRATSSVLVSDRPRKRPNNKQDLVQVSIEAQASKLRQSGTGGLERLETYPEEREQMRPAAFRIPGFLQETISLGLNPSGSRKRATSSVLVSDRPKKRPNNKQDLVQVAIEAQASKLRESGPGGLERLETYPEEILESGKQLKEDDIGRGKQVTEPLPPMFKVYDWDFLKARGTDGIMGNHKTGLSYQDRKLEYLFRTFEFQETDGFFWVRPTPRELSNIKKIFNKQRYIFRRNYRNGPKTRLLSELVLNLSDEKLALDRYPTFIDDMLDFERRMKARLNNIHNGASVTSDIAPLPRKVAAVSKYVSNMTKIVTFLTIFHLSLFKQHVGEKLTQKAVEEILVFVRDFWLQLELPNRDLLGENPWVTQISQMLRLEDEPATDYGERYRTELWYHKAWKIVQYWAEQNKKPLISKDGPESFPVELVNNMLMLSNPSYFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KFG0</accession>
    <name>E3KFG0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_09955</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178284">
        <property type="protein sequence ID" value="EFP82987.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327406.2">
        <property type="nucleotide sequence ID" value="XM_003327358.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KFG0"/>
    <dbReference type="GeneID" id="10544698"/>
    <dbReference type="KEGG" id="pgr:PGTG_09955"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09955"/>
    <dbReference type="HOGENOM" id="CLU_2672275_0_0_1"/>
    <dbReference type="InParanoid" id="E3KFG0"/>
    <dbReference type="OrthoDB" id="2498268at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP82987.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="75" mass="8652" checksum="3C1366446D20082A" modified="2012-04-18" version="2">MEASSTIHQLAHAILGAHEPCERLSGCPYRPVFINPENDQEFFVITLDRAHAWAREIQRRPHEVTIHIPPRSPLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KG32</accession>
    <name>E3KG32_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09099</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EFP83146.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327565.2">
        <property type="nucleotide sequence ID" value="XM_003327517.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KG32"/>
    <dbReference type="GeneID" id="10544859"/>
    <dbReference type="KEGG" id="pgr:PGTG_09099"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09099"/>
    <dbReference type="HOGENOM" id="CLU_698567_0_0_1"/>
    <dbReference type="InParanoid" id="E3KG32"/>
    <dbReference type="OrthoDB" id="2507672at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="73"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83146.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="395" mass="43994" checksum="1CF34856A8F7ECCD" modified="2012-04-18" version="2">MLHVRKVLKPGSANGKPGALMGNMASKIGRLSKSHDHNTSRNLSTHLPTSAREYYSAPQSYPDHRSEYDQPNSSSRFSNSTSAPEYIPAAESRRGSHLEYYFPNSPSRFSTPISAREYIPEAESHPDRYPAYHLPNLRPLSAPSSFSNAVFQAFSRSQMDIPQRAEHRVKQNVLIIQRPCWSRWFKRNPSQESAFYMESSNRESTLHNVEPPDEPHGRISLSRFPDPPSQNSNANFYKSNAISTAGNTPPSEILMCGGGNSRANSLVFFPIMDGPPESPEVSVPSSVYGQSFSTLPTSPSASLSPKSFNPEYHEELISPQSRATVISNSNSRHHRSIQVEISVLPQEGQSLTLGQISLVPQEYQSVTLDHPSTAIKSRIRTSWQPYNHSIVEEVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGF6</accession>
    <name>E3KGF6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Rieske domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_08567</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FAD-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83381.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327800.2">
        <property type="nucleotide sequence ID" value="XM_003327752.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KGF6"/>
    <dbReference type="FunCoup" id="E3KGF6">
        <property type="interactions" value="300"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KGF6"/>
    <dbReference type="GeneID" id="10545097"/>
    <dbReference type="KEGG" id="pgr:PGTG_08567"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08567"/>
    <dbReference type="eggNOG" id="KOG1336">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003291_4_2_1"/>
    <dbReference type="InParanoid" id="E3KGF6"/>
    <dbReference type="OrthoDB" id="6029at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.10.10">
        <property type="entry name" value="Rieske [2Fe-2S] iron-sulphur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050446">
        <property type="entry name" value="FAD-oxidoreductase/Apoptosis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028202">
        <property type="entry name" value="Reductase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017941">
        <property type="entry name" value="Rieske_2Fe-2S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036922">
        <property type="entry name" value="Rieske_2Fe-2S_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43557">
        <property type="entry name" value="APOPTOSIS-INDUCING FACTOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43557:SF2">
        <property type="entry name" value="RIESKE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14759">
        <property type="entry name" value="Reductase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00355">
        <property type="entry name" value="Rieske"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50022">
        <property type="entry name" value="ISP domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51296">
        <property type="entry name" value="RIESKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="6" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rieske" evidence="10">
        <location>
            <begin position="68"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP83381.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="613" mass="66667" checksum="C31F9025F1B9AE1F" modified="2012-04-18" version="2">MYPKNWKNIASYHFRPPTVRLKVVQPPPTRQVLLKSTFYLKTSSSPFSTAIPTLAPNMRLEAGNVSEWQDGMMKEIPLDPKADKSPKILVTKVMGKIHATSSQCTHYGASLINGVLASDGCLTCPWHGAKFSVNKDGDIEDAPGLNALQSFTVLIEDERVFVEVDPELVKATQFGRTPAKCKSSGQKAKDQPGVVVIGGGSGGHYFVEEIRRLNYNGNVTLISQEPNLPIDRTKLSKALITDPKKIAFRDEAFYRDTEIDLQIGKAVNKIDVEGQSVSLSDGSTIKYSKLVVATGGIPRTLPLDGADLEGISIMRAIPDAQKIQSILSGASPDKKKTIALIGSSFISMELAIFAAKKENVQVHVIGMDKIPLSKILGDKIGNAIKKSHEKNGVVFHLEANLEKFLPSESNPSHVGGVALKGGEVVKCDGVVLGVGVMPNTDLLKAAGIALEKDGSVVVDEYLRIKDSKARNTIYAIGDIAKYPDLLTDGALVRIEHWNVAGNHARAAAKNIAQEQKSEPLVAFTKPAIFWSAQGEQLRYVGTGKASQWQDVIIEGNADELNFVAYYYKDDEVVAIATMKKDPMMVQAAELFARKKFPSVSEIRKGMNLMSLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHP6</accession>
    <name>E3KHP6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_09534</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>myo-inositol(out) + H(+)(out) = myo-inositol(in) + H(+)(in)</text>
            <dbReference type="Rhea" id="RHEA:60364"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17268"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83821.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328240.2">
        <property type="nucleotide sequence ID" value="XM_003328192.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHP6"/>
    <dbReference type="GeneID" id="10532508"/>
    <dbReference type="KEGG" id="pgr:PGTG_09534"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09534"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_30_3_1"/>
    <dbReference type="InParanoid" id="E3KHP6"/>
    <dbReference type="OrthoDB" id="2544694at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005351">
        <property type="term" value="F:carbohydrate:proton symporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008643">
        <property type="term" value="P:carbohydrate transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000061">
        <property type="entry name" value="MFS sugar transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005828">
        <property type="entry name" value="MFS_sugar_transport-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050360">
        <property type="entry name" value="MFS_Sugar_Transporters"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003663">
        <property type="entry name" value="Sugar/inositol_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005829">
        <property type="entry name" value="Sugar_transporter_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00879">
        <property type="entry name" value="SP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022:SF68">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY (MFS) PROFILE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48022">
        <property type="entry name" value="PLASTIDIC GLUCOSE TRANSPORTER 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00083">
        <property type="entry name" value="Sugar_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="SUGRTRNSPORT"/>
    </dbReference>
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        <property type="entry name" value="MFS general substrate transporter"/>
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    </dbReference>
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    </dbReference>
    <dbReference type="PROSITE" id="PS00216">
        <property type="entry name" value="SUGAR_TRANSPORT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="74"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="125"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="188"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="346"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="380"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="427"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="10">
        <location>
            <begin position="17"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00049119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP83821.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="454" mass="49621" checksum="EE3FE0C7607CC476" modified="2012-04-18" version="2">MGQLYWGLTGDRLHLAVATLAGLGFLLFGYDQGVMGGLLTLPTFVKTFRSIDTTSVTLSPAQKKKNSTLQGTAVALYEIGCMIGALSCLYLGDRYGRRKVIFYGALIMICGATLQATAYHLAHFIIGRVVTGYGNGFITATVPTWQAECSKAHQRGKLVMIEGALITGGICLSYWVDFGMYFAQQSSASWRFPIALQIIFALIISLTVLSLPESPRWLIKQGRVTEAREVFSALQDSNKVDYFLVEKEIEDVQKSLALTGNSGLQDLFKMGRGRNFHRLVLGAVNQCFQQISGINLISFYAATIYQNYLGLSPMMSRIVAACNGTEYFLASWIAVYTIESYGRRKLMLLGSAGMGISMVGLAIATWAADPVSGKGSPGAAVVAAIFMFVFNSFFAIGWLGMTWLYPAEITPLEIRAASNGISTASNWIFNFIIVLITPIAFENIGYKTYSQPKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJ70</accession>
    <name>E3KJ70_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10065</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178290">
        <property type="protein sequence ID" value="EFP84345.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328764.2">
        <property type="nucleotide sequence ID" value="XM_003328716.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJ70"/>
    <dbReference type="GeneID" id="10545692"/>
    <dbReference type="KEGG" id="pgr:PGTG_10065"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10065"/>
    <dbReference type="HOGENOM" id="CLU_756802_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJ70"/>
    <dbReference type="OrthoDB" id="2503727at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="342"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="226"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="305"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="353"/>
            <end position="366"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84345.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="366" mass="40176" checksum="87848BC236ACC589" modified="2012-04-18" version="2">MSPTSECTATSSLPPPKSSFKTTSPGHSPIPNLSQTDNLRGHQRSNRVPSGGNRIPGGEPTQRSHMVSGGTQVRHSHYSIHINPKGESIRSGGSPAPLQVSRADQVGRKHVSTHLTQVQPSQLLKGVIPQQNRLSGLFGRSGGTSDIKPKENTSRFWQKKTKAVHNPCHSRAPLAPCLSFPVMMLSSISRLSSVESIKILPKFHRPTFLQNKGAPTKETKESINLSASSSSSSRSPKIHKVVHKYRTQCHKDDKEFLRAFLRDDGDDLDGEDDEDEDQSPNPLKATLKFFKRIPRALDLPHPTRSSSFGSSGSSSSNDSSQQSYSQSAASLITSSVASSNHYSWNRAQRATRNRTERECRLESLEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJQ4</accession>
    <name>E3KJQ4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10688</name>
    </gene>
    <organism evidence="2 3">
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        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328948.1">
        <property type="nucleotide sequence ID" value="XM_003328900.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJQ4"/>
    <dbReference type="GeneID" id="10546122"/>
    <dbReference type="KEGG" id="pgr:PGTG_10688"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10688"/>
    <dbReference type="HOGENOM" id="CLU_1482699_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJQ4"/>
    <dbReference type="OrthoDB" id="2511521at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="182" mass="20226" checksum="25B532379BE09776" modified="2011-01-11" version="1">MTSPTKQERPPLPFSTPPNVTSPVDSPPLRYNLPGFGIVEEIQTKKLNRQDIGEKSGPLGKGPNRPTGKAKPSLGTEWTHVPKRHSARIKREKQTNKPKVKATRPNAKKKPTAEDFRTTHHDAMDIDPQLTKNGPADDEPEPSKEVTKRQDQTEPPKGVTKSPDENDPKKPLSPKVIKIPSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJW1</accession>
    <name>E3KJW1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10745</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329005.1">
        <property type="nucleotide sequence ID" value="XM_003328957.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJW1"/>
    <dbReference type="GeneID" id="10546175"/>
    <dbReference type="KEGG" id="pgr:PGTG_10745"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10745"/>
    <dbReference type="eggNOG" id="ENOG502SEHV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1661673_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJW1"/>
    <dbReference type="OrthoDB" id="10378838at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="34"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="159" mass="17366" checksum="9E08BBC2EA13D6D1" modified="2011-01-11" version="1">MAHPQRPRETLSAAESVHSHRPGGTLSADEHLMSPASSSSHKSVPPRSHTVQPTTNNISFPTMNPSSAGPSAAQRPKRAPTPNFPSRDGADHSPVTPVACRSRSRTPSASTWIKRVVSRSSSRASLRKQYEQIPMSEEERIEAEQVKARKAKEALAEWN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>E3KK64</accession>
    <name>E3KK64_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="20">Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial</fullName>
            <ecNumber evidence="20">1.3.5.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="24" type="ORF">PGTG_10848</name>
    </gene>
    <organism evidence="24 25">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="25" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="25">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15 20">Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 20">
            <text>a quinone + succinate = fumarate + a quinol</text>
            <dbReference type="Rhea" id="RHEA:40523"/>
            <dbReference type="ChEBI" id="CHEBI:24646"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:132124"/>
            <dbReference type="EC" id="1.3.5.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="18">Flavinylated by SdhE, about 5% flavinylation occurs in the absence of SdhE.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 20">Carbohydrate metabolism; tricarboxylic acid cycle; fumarate from succinate (eukaryal route): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 20">Mitochondrion inner membrane</location>
            <topology evidence="1 20">Peripheral membrane protein</topology>
            <orientation evidence="1 20">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 20">Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="20"/>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84689.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329108.2">
        <property type="nucleotide sequence ID" value="XM_003329060.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KK64"/>
    <dbReference type="SMR" id="E3KK64"/>
    <dbReference type="FunCoup" id="E3KK64">
        <property type="interactions" value="208"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KK64"/>
    <dbReference type="GeneID" id="10526656"/>
    <dbReference type="KEGG" id="pgr:PGTG_10848"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10848"/>
    <dbReference type="eggNOG" id="KOG2403">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014312_6_1_1"/>
    <dbReference type="InParanoid" id="E3KK64"/>
    <dbReference type="OrthoDB" id="71672at2759"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER01006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045273">
        <property type="term" value="C:respiratory chain complex II (succinate dehydrogenase)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006121">
        <property type="term" value="P:mitochondrial electron transport, succinate to ubiquinone"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.700.10:FF:000001">
        <property type="entry name" value="Mitochondrial succinate dehydrogenase flavoprotein subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.80.40:FF:000002">
        <property type="entry name" value="Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.50.60:FF:000482">
        <property type="entry name" value="Succinate dehydrogenase complex, subunit A, flavoprotein (Fp)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.100:FF:000001">
        <property type="entry name" value="Succinate dehydrogenase flavoprotein subunit (SdhA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.100">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.80.40">
        <property type="entry name" value="succinate dehydrogenase protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003952">
        <property type="entry name" value="FRD_SDH_FAD_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011281">
        <property type="entry name" value="Succ_DH_flav_su_fwd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014006">
        <property type="entry name" value="Succ_Dhase_FrdA_Gneg"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01816">
        <property type="entry name" value="sdhA_forward"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01812">
        <property type="entry name" value="sdhA_frdA_Gneg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE 2 FLAVOPROTEIN SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632:SF51">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000171">
        <property type="entry name" value="SDHA_APRA_LASPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00504">
        <property type="entry name" value="FRD_SDH_FAD_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 20" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8 18 20" id="KW-0274">FAD</keyword>
    <keyword evidence="6 18 20" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="13 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="7" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="11 20" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="25" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 20" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4 20" id="KW-0813">Transport</keyword>
    <keyword evidence="5 20" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="22">
        <location>
            <begin position="62"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarate reductase/succinate dehydrogenase flavoprotein-like C-terminal" evidence="23">
        <location>
            <begin position="514"/>
            <end position="650"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="21">
        <location>
            <begin position="583"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="21">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="21">
        <location>
            <begin position="585"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="16">
        <location>
            <position position="340"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="67"/>
            <end position="72"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="90"/>
            <end position="105"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="308"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="407"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="442"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="453"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="458"/>
            <end position="459"/>
        </location>
        <ligand>
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Tele-8alpha-FAD histidine" evidence="19">
        <location>
            <position position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00059077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000171-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR611281-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU362051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="21">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="23">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="EMBL" id="EFP84689.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="25">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="650" mass="70877" checksum="DA2F12E700C083ED" modified="2012-04-18" version="2">MLRSKATSLLSRVKPSPTPLANRTFSSSSSRSRIISTQPVKASETSAKFSNGKYPIIDHEYDAIVVGAGGAGLRAAFGLAEAGLNTACITKLFPTRSHTVAAQGGVNAALGNMTEDDWRWHMYDTVKGSDWLGDQDAIHYMCKEAPQAVIELEHFGVPFSRTKEGKIYQRAFGGQSLKYGKGGQAYRCAAAADRTGHAILHTLYGQSLRHNTNFFVEFFALDLIMDDAGNCVGVTAYNQEDGTLHRFRAHQTVLATGGYGRAYFSCTSAHTCSGDGNAMVTRAGLPLQDLEFVQFHPTGIYGAGCLITEGSRGEGGYLLNSQGERFMERYAPTAKDLASRDVVSRSMTIEIREGRGVGPDKDHCYLQLSHLPPEVLHERLPGISETAAIFAGVDVTKEPIPVLPTVHYNMGGIPTNYHGQVLTQDPKTGADKIVPGLYAAGEAACVSVHGANRLGANSLLDIVVFGRACAKHISANMEPGKPHKAMSEDAGMKSIEDLDKLRNASGPKPTAQIRNDMQRVMQNDAAVFRTQSSLEEGVQKIHKVVDSFKDVGVTDRSMIWNTDLVETLELRNLLTCAAQTMSSAEARKESRGAHAREDFPEREDDSWMKHTLSWHNPESGEPVKLTYRDVISSTLDENECKSVPPFKRTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KK90</accession>
    <name>E3KK90_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_10874</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84715.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329134.2">
        <property type="nucleotide sequence ID" value="XM_003329086.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KK90"/>
    <dbReference type="GeneID" id="10526682"/>
    <dbReference type="KEGG" id="pgr:PGTG_10874"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10874"/>
    <dbReference type="HOGENOM" id="CLU_522891_0_0_1"/>
    <dbReference type="InParanoid" id="E3KK90"/>
    <dbReference type="OrthoDB" id="2500720at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="544"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP84715.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="572" mass="64526" checksum="FA4E0883D563FE52" modified="2012-04-18" version="2">MARERIISLRHLSQEYHGITPDGSPAQDDPRRESRLQAASRGEEEGNTIDEEAERLLSPDPRRSGSRPPKLVVEDYSLPPTDHRRRPGMTGGEREEKKSRHSPLIKSSGGPDIHEHPSIVTQHEVDEDEDDRRTLATADDQYDHPLLKHVWADPDGPIVSAAGQPDIDAEKAARFPLEAGDMGHFMNQASKISTRIDGLEAQLLENSPSFSNDPAFRTALYTQIDHHVVQQILLLQATHRLDSSPSFSPDIGDDNDHHHHDWKTVEKRRLTPSTLEIEHALKVEQIVALKTKAKSLVSGMIGRLKDEKQITTNQIEYEWNKELSQGSASSRHSRSVSTIVVPDARNPSIDSSPVSPDLQLFEKLQQIDHLPRASKSYSEEVQKILNLAVRSSTSYPEARKSSLFIRSKVDSVLQIHAEVDQLNRYLVQLSSDCSRQYGHEGTSSEEDEEDDDLSSISISLDGLEDEDKGRLEPQISGRKTNKIRFMLGDRPKKNPQSRSSFVEDLENNRVGNKEPQCHRLSYGSNSSYPISFHDNRSWLVRYRLTVCCSILAFVLLISMACLIYFNLKSHND</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3KL77</accession>
    <name>E3KL77_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_11221</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP85052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329471.1">
        <property type="nucleotide sequence ID" value="XM_003329423.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KL77"/>
    <dbReference type="STRING" id="418459.E3KL77"/>
    <dbReference type="GeneID" id="10527029"/>
    <dbReference type="KEGG" id="pgr:PGTG_11221"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11221"/>
    <dbReference type="HOGENOM" id="CLU_055324_1_1_1"/>
    <dbReference type="InParanoid" id="E3KL77"/>
    <dbReference type="OMA" id="CEQREST"/>
    <dbReference type="OrthoDB" id="76487at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023:SF4">
        <property type="entry name" value="GLYCINE-RICH PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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            <begin position="108"/>
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            <begin position="274"/>
            <end position="335"/>
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    </feature>
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        </location>
    </feature>
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            <begin position="317"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP85052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="335" mass="37885" checksum="22FFC9653E3DFF9C" modified="2011-01-11" version="1">MPTRSANWLPTEDQQLAKSWLKISEDPIISTGQKRAEFFKRVAEDYNDYSGGIERDGTMAMHRWGHIQKACLKFSGIYHKLKNNRPSGSVLSNLLPDTKKAFYEQEGKQFIFELAWMVVKDSSKFNSLAQTRGDQSKQATTPAPTPTPSTPAAQSDAALGNSQPEDIAQIRSNNSWNRPAGIHATKRAMKQEHFNAKKIKVMSERSSNYRERTIAMKKTNKIRQQVAKAEVNQVNMEIMSRKEDELPDDVSREFLRLQKGEILEDLREQIRERQKLAAAKEKQTEASTNEALAASSERDSTFEDDPSSNAEDSQYIGDEDEDDIADLDPLLDTLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KMI1</accession>
    <name>E3KMI1_PUCGT</name>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178295">
        <property type="protein sequence ID" value="EFP85506.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329925.2">
        <property type="nucleotide sequence ID" value="XM_003329877.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KMI1"/>
    <dbReference type="GeneID" id="10544058"/>
    <dbReference type="KEGG" id="pgr:PGTG_11862"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11862"/>
    <dbReference type="HOGENOM" id="CLU_041944_0_0_1"/>
    <dbReference type="InParanoid" id="E3KMI1"/>
    <dbReference type="OrthoDB" id="2512529at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="199"/>
        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
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        </location>
    </feature>
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        <location>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="145"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85506.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="466" mass="50096" checksum="3337EE1B7199ACF5" modified="2012-04-18" version="2">MSVVVSSASESEYQVSNQSPSPAPDTPGPRSRVTTQSSTRESQTGALRPSARHTQGDSDQSPSPPPDTPGPHTRVTARSNNHTRVTAQSNNREAQTGPIRPAARRTHGNGGRLPARPQRGASTTSAAPPARGASRPRPANLPAVPSRARPAASSSSTRRATAIQAPGNEADPQAVRAPGNEADPQAVQAPGNEADPQGAWPPATVHLTLDDEPINDEFISEVDAIFQLGGPYADLGEQLAYVPLPRQYVVGMYGLLSVRQAIERLRHDILNAPRGRGAQSAAVSTVFQAENFHYVAVFSEKINEQSDEFKIRYLPRGYSDPDPAAQRSVTTYVRGKLRNSRGKMRDLLLTNINMRSELPVPTISSLLTLMRSSLIPPLPNNAPATDTTAQGRRDYALLKARAANTDQHSFYQLVTRYDRELFGGEVVFSNIDRDSIALPTHEDIEALVASNAGVDIEAVADPGEDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KMS9</accession>
    <name>E3KMS9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    <organism evidence="2 3">
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EFP85778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="AlphaFoldDB" id="E3KMS9"/>
    <dbReference type="GeneID" id="10544353"/>
    <dbReference type="KEGG" id="pgr:PGTG_11107"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11107"/>
    <dbReference type="HOGENOM" id="CLU_033090_2_1_1"/>
    <dbReference type="InParanoid" id="E3KMS9"/>
    <dbReference type="OrthoDB" id="2496543at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
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    </dbReference>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="430" mass="48779" checksum="89672149A5111973" modified="2011-01-11" version="1">MSDSNSETGNSSTSDDEWGASQTSETGPKSKTDLVVEALEYMDRKYMFSSEREADADKHLTIDDMGEKLKRLDELETSLLPSMKELIDQLLDALDIKGSSDYPGPQPDLDLTLEILSKLDKNLKRTVSSGVFLALESPLPDEDHDHHLKKAKIFRCTHLLSEIECLVVDEIYDLLVCCMSVVQWCESLSDCSDEALLQSEVLLSRREALMSATQCQHEIDRLIKFSQKSDLAILQVTWLPPADLLDKVLVNFTNLTNPRFERNAWIVEDQSQEREAPREEIVQVAKSTIPIIKLARILLGKLSQLSRKEVPFTLHSAIDSKSVDQLTSAQHSIAVQLDRIGFTFLKNHRTNQALEPIFDQAHAVVKKISWIVESHLVDIAFHLVPLPHLLLNGVNSPTRHFRAQFYPWKQLWDVAVSRLLDLLCSFETEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KN49</accession>
    <name>E3KN49_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_11014</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EFP85685.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330104.2">
        <property type="nucleotide sequence ID" value="XM_003330056.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KN49"/>
    <dbReference type="STRING" id="418459.E3KN49"/>
    <dbReference type="GeneID" id="10544251"/>
    <dbReference type="KEGG" id="pgr:PGTG_11014"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11014"/>
    <dbReference type="HOGENOM" id="CLU_1627894_0_0_1"/>
    <dbReference type="InParanoid" id="E3KN49"/>
    <dbReference type="OrthoDB" id="2502666at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023:SF4">
        <property type="entry name" value="GLYCINE-RICH PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP85685.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="163" mass="17717" checksum="A7188AFFBAEEEA63" modified="2012-04-18" version="2">MAKGEIEIVPTNNKTTKKKRAPNWLPLEEEQLAISWVHVSEQPEFSNNQTGTMWTSLNTATLKFAAIYNAIERNPPSGSSPEDWMATAMTVYAGQTKGIAFGSVAAWQKVQYCPKCKAPATPTGICTPSLRDGSLISLPIGGKAAKKRRIEGYKDDEMLASGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3KN55</accession>
    <name>E3KN55_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rhamnolipids biosynthesis 3-oxoacyl-[acyl-carrier-protein] reductase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_11020</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EFP85691.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330110.2">
        <property type="nucleotide sequence ID" value="XM_003330062.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KN55"/>
    <dbReference type="STRING" id="418459.E3KN55"/>
    <dbReference type="GeneID" id="10544257"/>
    <dbReference type="KEGG" id="pgr:PGTG_11020"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11020"/>
    <dbReference type="eggNOG" id="KOG0725">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_1_1"/>
    <dbReference type="InParanoid" id="E3KN55"/>
    <dbReference type="OrthoDB" id="2898618at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052178">
        <property type="entry name" value="Sec_Metab_Biosynth_SDR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43618">
        <property type="entry name" value="7-ALPHA-HYDROXYSTEROID DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43618:SF8">
        <property type="entry name" value="7ALPHA-HYDROXYSTEROID DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00106">
        <property type="entry name" value="adh_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP85691.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="283" mass="29548" checksum="A1C6C45CDF6D68D9" modified="2012-04-18" version="2">MASRDDPLSINNLLGVKGRVAVITGGGSGLGEMMAKAWVVNSGHVIIASRKEQALSKVCQELNALGTGKADYVVGDVSSKAGCLALCAEIKKKVNKIHVLVNNSGVSWGAPYDNVPEKEGWDKVLSMNVKSVFYMTSELTSLLAKDATNLDPGRVIVVSSVAGLTSKAESSGLGAEGHGLWSYNASKAAAISLSNNLAVTLAPKFITVNAICPGVYPSRMTAYGLKKQSDLLNSQHPMGRIGTPEDMAGVFLFLTTRAGAHITANYILTDGGSLASTSGYTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNC2</accession>
    <name>E3KNC2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11087</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EFP85758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330177.1">
        <property type="nucleotide sequence ID" value="XM_003330129.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNC2"/>
    <dbReference type="GeneID" id="10544324"/>
    <dbReference type="KEGG" id="pgr:PGTG_11087"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11087"/>
    <dbReference type="HOGENOM" id="CLU_3069765_0_0_1"/>
    <dbReference type="InParanoid" id="E3KNC2"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="53" mass="5735" checksum="60D2271231E036A6" modified="2011-01-11" version="1">MMSWRSSWDGSGGSCAHQRNPKTSKLQTHPIKGPGWPSGNHHPRKLGVSPATT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNK6</accession>
    <name>E3KNK6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_11637</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178297">
        <property type="protein sequence ID" value="EFP85881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330300.1">
        <property type="nucleotide sequence ID" value="XM_003330252.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNK6"/>
    <dbReference type="GeneID" id="10544463"/>
    <dbReference type="KEGG" id="pgr:PGTG_11637"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11637"/>
    <dbReference type="HOGENOM" id="CLU_058865_0_0_1"/>
    <dbReference type="InParanoid" id="E3KNK6"/>
    <dbReference type="OrthoDB" id="2498244at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP85881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="340" mass="39398" checksum="D5360E25D8D80879" modified="2011-01-11" version="1">MVKNLKNGKAPSNIQTLNKALSSIIPPSNSHFSRCIASFCRLVLNIKKLEEQHWQNSPSAEQLQHANNLPLTITSHPIQSRIKLSPVKPHVLHGISLDCRELFLADLKAANFPHPTFAWHEPWDSHWNQLFSAFLLKHWNHCYKYGAFANFPFNSKHNNSVNSMAVLKRWFVGKSNDIRKNKYSPVSVMKKAIQVKKSKWRKQTDIFSEPTCCSDTEQTDDGNFHRVQCPWRSTLFTELAYHLDKFFEKNKAEKLGKKYYTNTTSIWLLRQTSELREKIINVPSGLPRNCYHPNFLASLNETDINVLRIKDKIDLQAIIKEVSTQRYTQPDTIKEVSLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="86" xmlns="https://uniprot.org/uniprot">
    <accession>E3KP08</accession>
    <name>E3KP08_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 15">DNA replication licensing factor MCM4</fullName>
            <ecNumber evidence="3 15">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PGTG_11989</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 20">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.12"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the MCM2-7 complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the MCM family.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="3 15"/>
    <dbReference type="EMBL" id="DS178298">
        <property type="protein sequence ID" value="EFP86033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330452.1">
        <property type="nucleotide sequence ID" value="XM_003330404.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KP08"/>
    <dbReference type="FunCoup" id="E3KP08">
        <property type="interactions" value="596"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KP08"/>
    <dbReference type="GeneID" id="10540035"/>
    <dbReference type="KEGG" id="pgr:PGTG_11989"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11989"/>
    <dbReference type="eggNOG" id="KOG0478">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000995_7_1_1"/>
    <dbReference type="InParanoid" id="E3KP08"/>
    <dbReference type="OMA" id="AFFKCNV"/>
    <dbReference type="OrthoDB" id="10251574at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071162">
        <property type="term" value="C:CMG complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097373">
        <property type="term" value="C:MCM core complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005656">
        <property type="term" value="C:nuclear pre-replicative complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030875">
        <property type="term" value="C:rDNA protrusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033679">
        <property type="term" value="F:3'-5' DNA/RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990518">
        <property type="term" value="F:single-stranded 3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003727">
        <property type="term" value="F:single-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902975">
        <property type="term" value="P:mitotic DNA replication initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006267">
        <property type="term" value="P:pre-replicative complex assembly involved in nuclear cell cycle DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006279">
        <property type="term" value="P:premeiotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17755">
        <property type="entry name" value="MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000003">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1640.10:FF:000011">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000217">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1640.10">
        <property type="entry name" value="mini-chromosome maintenance (MCM) complex, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008047">
        <property type="entry name" value="MCM_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027925">
        <property type="entry name" value="MCM_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF66">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14551">
        <property type="entry name" value="MCM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21128">
        <property type="entry name" value="WHD_MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01660">
        <property type="entry name" value="MCMPROTEIN4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 15" id="KW-0235">DNA replication</keyword>
    <keyword evidence="10 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8 15" id="KW-0347">Helicase</keyword>
    <keyword evidence="7 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="17">
        <location>
            <begin position="567"/>
            <end position="782"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="503"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="60"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="84"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="135"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00073498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU368062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EFP86033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="996" mass="108962" checksum="CD4FC3EE5B248EB8" modified="2011-01-11" version="1">MSTPSNNEERMRLQLSSVPETSSSPMVFPSSSAGHPTSDAAPHSASRVQGIRRTAQSHINSSSPLRFPSSSNSTITGRRSGRANPNPSSEPLFFPPSSGEGTPRASRPSNRRGDIHSTVPSLSSPSAHPRLHAQVPSSSAGLGQTPNVPGSDLPSQHDTSSQAVPAEPETQTVIWGTLVSLQDSMQTFRQFLQGFKRKYRLAFDLKLSLDDASMLSNGDQLVYVDYMRKMRLTSQTNLNLDLINLLAFPPTKNLYNQILNYPQELIPICDQVLKDCVIELAEEDRERGDLESEAVPGDEEIAEMEGRIYKIRPFGYAGQSPESTVTTAAGQPVHRGVNMRLLNPGDIDKIVAIKGLVIRATPVIPDMKLAFFRCLSCGHVTTVEIDRGKIAEPQRCPREVCNQPGGMSLIHNRCEFADRQVIRLQETPDEVPDGQTPHSVSLCVYDELVDSAKPGDRVEITGIFRSVAVRVNPKQRVIKSLFKTYLDVLHIKRSDSRRLGVDLSTRSTDGRTNEGVVGVGGDDEEEQTSGGGVSSSEDNLFRTGADNPSNRAELEEKLLELSRRPEIYEILSRSLAPSVWEMDDVKKGILLQLFGGTNKTIGGSAASRNGSGGPRYRGDINVLLVGDPGVSKSQILQYVHKIAPRGVYTSGKGSSAVGLTAYVTRDPDSRQLVLESGALVLSDGGVCCIDEFDKMSDATRSVLHEVMEQQTVSIAKAGIITTLNARTSVLAAANPVGSKYNLAWPITKNIDLPPTLISRFDLLYLVLDKVDEASDRKLAKHLVALYLEDRPETGGLDILPVQTLTAYISFARNHIHPVLTEDACDALVRAYVTMRKAGEDSRTSERRITATTRQLESMVRLSEAHARMRFSETVDVADVEEANRLIREALKESATDPITGLIDLDLLQTGQGLHQRKLQEDLKREILKLLDSAQTQSTGGVRWPILFELLANQSSLPIDPSEFADVLKALADEGLVSISGERDKRLIFRTSSLNPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPF8</accession>
    <name>E3KPF8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12139</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178298">
        <property type="protein sequence ID" value="EFP86183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330602.1">
        <property type="nucleotide sequence ID" value="XM_003330554.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KPF8"/>
    <dbReference type="FunCoup" id="E3KPF8">
        <property type="interactions" value="161"/>
    </dbReference>
    <dbReference type="GeneID" id="10540192"/>
    <dbReference type="KEGG" id="pgr:PGTG_12139"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12139"/>
    <dbReference type="HOGENOM" id="CLU_152718_0_0_1"/>
    <dbReference type="InParanoid" id="E3KPF8"/>
    <dbReference type="OrthoDB" id="439808at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00590">
        <property type="entry name" value="RRM_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RRM" evidence="1">
        <location>
            <begin position="29"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="105" mass="11165" checksum="816E53237C7884D8" modified="2011-01-11" version="1">MSSPEIVYDDTATFCSQHTANQPSRIVGVANLILGTSEADIRAAFCEFGRIQFCFIPLSAPTTAIIVFSTDAEAAAGVDGMDGAIADGNSLLFFYSLGYMIKQDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQ84</accession>
    <name>E3KQ84_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Protein BIG1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12415</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178300">
        <property type="protein sequence ID" value="EFP86459.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330878.2">
        <property type="nucleotide sequence ID" value="XM_003330830.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQ84"/>
    <dbReference type="GeneID" id="10540474"/>
    <dbReference type="KEGG" id="pgr:PGTG_12415"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12415"/>
    <dbReference type="HOGENOM" id="CLU_1034704_0_0_1"/>
    <dbReference type="InParanoid" id="E3KQ84"/>
    <dbReference type="OrthoDB" id="2499821at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="231"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86459.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="277" mass="30257" checksum="E1207EFEF1F8D1EF" modified="2012-04-18" version="2">MHNSNFRCTYARIFSTVLLFGLPNKNHAFMETSSFLAWSNAPGASLQEFFGQGLPDRGFQAATDQLTFASRSEPLCPTGASSLTIFDLSESDKRSELNNGFLSSQTITQEFNALPEPSRFWTTHFPISLADRWVAELPSIWSGLCHGQVSVIKVKTGEDLDAAFLKASQEGDQPRLVILTTSPAPLDASSRLIERRGLSENISSSSKPISSEALPDPKTGVFWRYNLLSDYLIVGVLVLILLFIPPVVLGAFALQSIESPKGLKSKMVGQVSEVKGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQL2</accession>
    <name>E3KQL2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycosyl transferase CAP10 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_12969</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178301">
        <property type="protein sequence ID" value="EFP86587.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331006.2">
        <property type="nucleotide sequence ID" value="XM_003330958.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQL2"/>
    <dbReference type="GeneID" id="10540687"/>
    <dbReference type="KEGG" id="pgr:PGTG_12969"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12969"/>
    <dbReference type="HOGENOM" id="CLU_005027_3_1_1"/>
    <dbReference type="InParanoid" id="E3KQL2"/>
    <dbReference type="OrthoDB" id="541052at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006598">
        <property type="entry name" value="CAP10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051091">
        <property type="entry name" value="O-Glucosyltr/Glycosyltrsf_90"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203:SF118">
        <property type="entry name" value="BETA-1,2-XYLOSYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12203">
        <property type="entry name" value="KDEL LYS-ASP-GLU-LEU CONTAINING - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05686">
        <property type="entry name" value="Glyco_transf_90"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00672">
        <property type="entry name" value="CAP10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase CAP10" evidence="3">
        <location>
            <begin position="284"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP86587.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="573" mass="67056" checksum="0ED3B3D5A1B03651" modified="2012-04-18" version="2">MARYHLIVPPRPHTLLTCLIFVGTLGLLFYLPFIKIQANTCTPTFRLPTRQTFFATENGLIVFATPIRNNTKIKHPIESLIANAKTEWDQMLQRQSKTLAMAVQEYQRRNNQRLPPKGFERWWQFAVDNQVGLVDEYDQISKDLEPFWAIEPSRLRKLSNQLQTKQNDRRLIFQVDSEGKVNRTGAFGASARAKDLSNLMESYQKYSVNAMKPFTMVIGADDQAEIKTTWRERSRLLELSKRHEFIEEAEDVQLSETHSPYDDCPPDSSPRSLGKGYSSFVFDHLQSMDICSSLEQVPIHGSLVNRHHSPFQPITPLFSFSKTSLDSDIRVTPLEQYQSTYVHVYDWDDPRRIGKAFWRGSTTGFNENPSWKESHRLRLHQLTRPAKERKQLIDGSHVDLAKVNSEWFDIEFVGKPIQCEPPACQELKDMINFQEWVGQEQANLYKYALDVDGNGWSGRFHKLLSSNRVVIKNTIFPEWYADRIQPWYHYVPVKTDYSDLHDIIHYLKTRDDLAKQIAQNGLDYSQRYWRTQDMAAYMFRLSLEWSRLYNRETCDPSMENHHCESHDFDLNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3KTZ6</accession>
    <name>E3KTZ6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein YAE1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Protein yae1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_13477</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the YAE1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178308">
        <property type="protein sequence ID" value="EFP87691.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332110.2">
        <property type="nucleotide sequence ID" value="XM_003332062.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KTZ6"/>
    <dbReference type="STRING" id="418459.E3KTZ6"/>
    <dbReference type="GeneID" id="10538924"/>
    <dbReference type="KEGG" id="pgr:PGTG_13477"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13477"/>
    <dbReference type="eggNOG" id="KOG4774">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1299960_0_0_1"/>
    <dbReference type="InParanoid" id="E3KTZ6"/>
    <dbReference type="OrthoDB" id="20086at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019191">
        <property type="entry name" value="Essential_protein_Yae1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038881">
        <property type="entry name" value="Yae1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18829">
        <property type="entry name" value="PROTEIN YAE1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18829:SF0">
        <property type="entry name" value="PROTEIN YAE1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09811">
        <property type="entry name" value="Yae1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Essential protein Yae1 N-terminal" evidence="9">
        <location>
            <begin position="41"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF09811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP87691.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="180" mass="20468" checksum="8A1B2F0691B1F7B9" modified="2012-04-18" version="2">MESVSSEDWLEESLQVPQHQGHQQISEVEYTRLAEQFHTTGYREGIHKGKELSIQEGFNHGFAAGAQRGKRLGSLRGRASATLNLLLKQMEEDARVDRVRKLIRDLNQYGIVQDPNQSSRANELEDLTSRMDGLMTPSNPTQPQSVTTQYQKEQDDQLLDRCQFELDAILASMNIWLPGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KV39</accession>
    <name>E3KV39_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_12586</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178311">
        <property type="protein sequence ID" value="EFP88139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332558.1">
        <property type="nucleotide sequence ID" value="XM_003332510.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KV39"/>
    <dbReference type="STRING" id="418459.E3KV39"/>
    <dbReference type="GeneID" id="10535362"/>
    <dbReference type="KEGG" id="pgr:PGTG_12586"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12586"/>
    <dbReference type="eggNOG" id="ENOG502SPIA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1321465_0_0_1"/>
    <dbReference type="InParanoid" id="E3KV39"/>
    <dbReference type="OrthoDB" id="2507701at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP88139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="208" mass="23283" checksum="D03AD7AFB8976E36" modified="2011-01-11" version="1">MCQMQHSRLDPKLRDLTTNKPSQLLFLLTLIAQPTQLIPQHSLTTMLQAAKLSLVTRKFLKKVGKEVQYCNAKLMDVERTGKKITKIIPIRTLTFQPLKAWLMHKLWLPGFEALLDSNLKHKNSGSGTMGDVWDGSVWKNLVGPQDSDEIFTSRPGHLVFSLYVDWFNPYGNKSGGKSAPVSPENLGFKPKINVSSSSVWDRIHAGAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3KW63</accession>
    <name>E3KW63_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Surfeit locus protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_14116</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SURF4 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178314">
        <property type="protein sequence ID" value="EFP88538.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332957.2">
        <property type="nucleotide sequence ID" value="XM_003332909.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KW63"/>
    <dbReference type="STRING" id="418459.E3KW63"/>
    <dbReference type="GeneID" id="10541004"/>
    <dbReference type="KEGG" id="pgr:PGTG_14116"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14116"/>
    <dbReference type="eggNOG" id="KOG3998">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056195_0_0_1"/>
    <dbReference type="InParanoid" id="E3KW63"/>
    <dbReference type="OrthoDB" id="7859621at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005793">
        <property type="term" value="C:endoplasmic reticulum-Golgi intermediate compartment"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002995">
        <property type="entry name" value="Surf4"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02077">
        <property type="entry name" value="SURF4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="112"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="265"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="303"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP88538.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="36768" checksum="F0628DF5CC1B6E7E" modified="2012-04-18" version="2">MSTYTPVPLKDLDHNNPYHSNAGGPLPSTGPSRPPFDDQGESRLTRATQLLRKYSAPIEALIENLSRPVKPHLNKLARFLLVSTFLEDALRISNQFYEQKEYLIDDQEYHDWFVVGFLAANVILMVVCSILIIFKKWALFSVLGLVGVIVAQGWVYDLLTDGTFVCLNLSIMGALMLAISDSIVSKSVGGSVRGMAGLPDISEDVARKRRTYLQLSGRILLVVFFVGHALAAYLELAFDSFSFLHLAAAALAVLACLLVAVGFKAAWSATFLVILTSVVNVLSNDWWSKPKDHPERDFRRFDFFQALSVVGGMILLINQGPGGISFDAKRKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3KWV4</accession>
    <name>E3KWV4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Enoyl-CoA hydratase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_14737</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178316">
        <property type="protein sequence ID" value="EFP88771.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333190.2">
        <property type="nucleotide sequence ID" value="XM_003333142.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KWV4"/>
    <dbReference type="STRING" id="418459.E3KWV4"/>
    <dbReference type="GeneID" id="10541722"/>
    <dbReference type="KEGG" id="pgr:PGTG_14737"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14737"/>
    <dbReference type="eggNOG" id="KOG1679">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1245893_0_0_1"/>
    <dbReference type="InParanoid" id="E3KWV4"/>
    <dbReference type="OrthoDB" id="410701at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF171">
        <property type="entry name" value="SD19268P"/>
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    </dbReference>
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        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ClpP/crotonase"/>
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    </dbReference>
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        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP88771.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="187" mass="20313" checksum="B6A8A862AF36FE14" modified="2012-04-18" version="2">MIRSSSTAVFKLRLNFALFNARPAVYNSSLPGLYRRRYSTSKPVVYLDHPPESAGLEGITYLNLNRPEAKNAISLELLDQMFTCLGKVRFDGTRVLILKSSVPGSFCAGADLKERRRMSKVEVSKFLHDLRNTLHELDTLPMPTIAAIDGPALGGGLELALACDLRVAGPGATKLGLTETKLGIIPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3KWW3</accession>
    <name>E3KWW3_PUCGT</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14195</name>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178317">
        <property type="protein sequence ID" value="EFP88856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003333227.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KWW3"/>
    <dbReference type="STRING" id="418459.E3KWW3"/>
    <dbReference type="GeneID" id="10541972"/>
    <dbReference type="KEGG" id="pgr:PGTG_14195"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14195"/>
    <dbReference type="HOGENOM" id="CLU_421579_0_0_1"/>
    <dbReference type="InParanoid" id="E3KWW3"/>
    <dbReference type="OMA" id="NHANAGH"/>
    <dbReference type="OrthoDB" id="10546552at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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        <location>
            <begin position="21"/>
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        </location>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="74"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
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            <begin position="93"/>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
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        <location>
            <begin position="298"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="650" mass="72400" checksum="324FFEFD880BCF5A" modified="2011-01-11" version="1">MAPRNIRRSTPTPQLPPSRGLQSSNLASRLNRSRRASAQRDLDSLLQDSQRHLRLSPGPASTRSQLTLPGESIASVNLNPSTTSRFNLDSNLPLPPSPSPPPASSKGPPARPMAFQSDPGDPSMSDLFGYSDPYQPNGLAEQATYYDPLQHYEAPYQHHQHPHNQRSQHHNLQQSHQQPHLLHAQQPQIYQQLAQPHQSGQQSMYQPPPPKPVQSQRPVQHQHHSSGLPLAPLQGKQLPSWQQLNADAEASLAKQTAPVTRKKREPRKKKNDQTQPKQAATSVPTVSASLNPTPAKESQTASTVKTSTSTQTQTASLQAAATASDTEHLPSPATTTLPATTTLPATTTLPATTTLPATSSIIPQHRSHSGFPLNSTRVPASKRPLEDDVVAEFEKENLDELRRIARRIAENHRLTAEQKLEFDQVYDRYQRDLHKTAIKYKLDPAVPLSYVGNKTRIRGPSSYNNFCVYNPEASPIHHDYTKPVNERAVLTAALWAKLDKQAKAKYRDPDYLETFPNPYIGIRKAAEACANQEDQTDRVGPKKSRKQYVNMKPDRWAKKVLLDLKRIGEAYQVEGFLVFVTRHRKRTAIIEGGSTLGQHYLDITADDKDVIGPAQDFADFVNGCKVIKKHTAKEAKEREKGVGRWEIRQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KX26</accession>
    <name>E3KX26_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14135</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178317">
        <property type="protein sequence ID" value="EFP88796.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003333167.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KX26"/>
    <dbReference type="GeneID" id="10541748"/>
    <dbReference type="KEGG" id="pgr:PGTG_14135"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14135"/>
    <dbReference type="HOGENOM" id="CLU_1611596_0_0_1"/>
    <dbReference type="InParanoid" id="E3KX26"/>
    <dbReference type="OrthoDB" id="10287862at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88796.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="165" mass="17902" checksum="4416E10F9470B2C2" modified="2012-04-18" version="2">MGDASHWGALHPQSREALLSQSRDGILLMVLRRQAHPLRQLPDDGGAGSGDLGVSPQNESSLLTSQRHQRVRTPDEHHSPTIGCTFLVITPRVGSLAVAEAIIYVAQAELADGKTSYCNDHGLDCALHGSSSRESAIISLRQLAPIKHLYEHVLSRSRNSMNEPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KY72</accession>
    <name>E3KY72_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14993</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178320">
        <property type="protein sequence ID" value="EFP89152.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333571.2">
        <property type="nucleotide sequence ID" value="XM_003333523.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KY72"/>
    <dbReference type="GeneID" id="10546705"/>
    <dbReference type="KEGG" id="pgr:PGTG_14993"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14993"/>
    <dbReference type="HOGENOM" id="CLU_128888_0_0_1"/>
    <dbReference type="InParanoid" id="E3KY72"/>
    <dbReference type="OrthoDB" id="10293722at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172311" evidence="1">
        <location>
            <begin position="25"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89152.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="160" mass="17380" checksum="116D93108410E40F" modified="2012-04-18" version="2">MLRVCAGTAITLLTLSVQLVVLEARHTSLVPRDGFVKNCVRFLGEHYDDSSKMICEDNHTGYWCYKDQCTSGDLPGSHAAAPFSTMTWSGCKRISLGDILGAEGVTASHAISVHFLPGQKVAVHGSQNGLSGIFDFECHWNSPSDHNARTPCESIGNHFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>E3KYU1</accession>
    <name>E3KYU1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 13">Phosphomevalonate kinase</fullName>
            <ecNumber evidence="3 13">2.7.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGTG_15329</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(R)-5-phosphomevalonate + ATP = (R)-5-diphosphomevalonate + ADP</text>
            <dbReference type="Rhea" id="RHEA:16341"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57557"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.2"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:16342"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 13">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the GHMP kinase family. Mevalonate kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.2" evidence="3 13"/>
    <dbReference type="EMBL" id="DS178322">
        <property type="protein sequence ID" value="EFP89487.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333906.2">
        <property type="nucleotide sequence ID" value="XM_003333858.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KYU1"/>
    <dbReference type="FunCoup" id="E3KYU1">
        <property type="interactions" value="96"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KYU1"/>
    <dbReference type="GeneID" id="10547374"/>
    <dbReference type="KEGG" id="pgr:PGTG_15329"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15329"/>
    <dbReference type="eggNOG" id="KOG4519">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022059_1_0_1"/>
    <dbReference type="InParanoid" id="E3KYU1"/>
    <dbReference type="OrthoDB" id="10262935at2759"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00099"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004631">
        <property type="term" value="F:phosphomevalonate kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006696">
        <property type="term" value="P:ergosterol biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000121">
        <property type="entry name" value="Phosphomevalonate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016005">
        <property type="entry name" value="Erg8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035102">
        <property type="entry name" value="Phosphomevalonate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31814">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31814:SF2">
        <property type="entry name" value="PHOSPHOMEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017288">
        <property type="entry name" value="PMK_GHMP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 13" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10 13" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-0752">Steroid biosynthesis</keyword>
    <keyword evidence="11 13" id="KW-0753">Steroid metabolism</keyword>
    <keyword evidence="5 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="14">
        <location>
            <begin position="183"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR017288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EFP89487.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="549" mass="60538" checksum="A1593A4B5ECAE5BF" modified="2012-04-18" version="2">MFDSRETIVSCPGKVLIAGGYLVLDREYPGIVVGTSSRFYTVIRPAQSPLGRSPEQQGSLKYQQIRLSIRSPQFIDAEWSYLISLSPQADLIIKPEASSSQNKFVELAVRESLKLSLALQNPDQFILQPPSEKPHQNGHSANQPYPMTITILGDNDFYSQPRQDHAPVPAFNRLNTTLKDVHKTGLGSSAAMVTSLCSAILIYFAPSIKPLSRSTKLLLHNLSQYVHSLAQGKVGSGFDVSAAVYGTHVYRRFSPACLDGLLGSSGDQAHLTPNQIWRTLDPKINESWVDASTAPVIERFSIPKFTTLLLADVDAGSHTPSMVGQVFKWKNSESESANQIWKELSIQNNQLKVAFERLERLSSLNEQNCLSEVLKLSSDSNFYLQNDPSPVTETDDVRTLFIKVSSITKSIRQLMKKMGTQSNVPIEPDSQTQLLDECEKRHGVIGSGVPGAGGYDAIWVLIFTPPSLPECSTAEKVDGRSGNIQHVIEFLNHWPHSSVRVLSSDSWVVGGSTNALRESSDDGHEDDGIKIFNDLQLVDCLKSQLNLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZY8</accession>
    <name>E3KZY8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15825</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP89869.2"/>
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        <property type="nucleotide sequence ID" value="XM_003334240.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZY8"/>
    <dbReference type="GeneID" id="10545601"/>
    <dbReference type="KEGG" id="pgr:PGTG_15825"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15825"/>
    <dbReference type="HOGENOM" id="CLU_044887_0_0_1"/>
    <dbReference type="InParanoid" id="E3KZY8"/>
    <dbReference type="OrthoDB" id="2509728at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="201"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="286"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="323"/>
            <end position="341"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89869.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="408" mass="45666" checksum="F8A388756686F19A" modified="2012-04-18" version="2">MMDFAFVAVLVYETPLLFVNTGILMAISQFIGSIAAQAFIFIQIKTSHSSDYALKSPLASPMGVMFMCEVLIYWSLSHCFLVTIYYDRKTHGEITQGTRTWISPPAVINFVFLSFPICVVAATIALFTWLSSGFTHFMVAVVKVLDALRQGSSAWDQLKVPSTSSEEKLHLTTQLIQVVSEAKNFTTDVNIRSENLINCFHLVQCVLLIIICTTSLVFIVSFWVLVHKFRRRHRQSDKASSRSIYLRRWGKRNNPRNASGEQSTTSSNLNPSFIDVVKSDRQFLHLALRAISIIIAMLTTISLLLLGIIRTADVVKYPYWRGLSTWLTTVSGTWAAIPIAWQCWRLYQEQSGGASQSLSNSNDTALIGVPGSKNADPGRPTQDGAETIEIKIELDPKAIHPTETYEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
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    <name>E3L003_PUCGT</name>
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        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="ORF">PGTG_15840</name>
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178326">
        <property type="protein sequence ID" value="EFP89884.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="GeneID" id="10545618"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15840"/>
    <dbReference type="HOGENOM" id="CLU_030979_2_1_1"/>
    <dbReference type="InParanoid" id="E3L003"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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            <end position="210"/>
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    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
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            <end position="263"/>
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    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="297"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89884.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="361" mass="41498" checksum="7D92951E700BF6D2" modified="2012-04-18" version="2">MHGATGAVSFFNKPARNLLHERVGRALLVNTVHRSLRRGVSRLPSTHAMWNDLFARFHLVSRAAQLNLFRRLISFNVVDHSTTAQMSTHIANILDEMTDARMLFTREYLAGLVLQNGLHSEPALQEEFNRQIEINFQTATPERPAMSFESMVRLIDIIRRQQQFQSINRDSQRPTPLVMQAEAQQPEQPFPDAQTELPQFPAHPDNQPDAHDFMAMQAGLCWQCRSHKHFLRNCPFCARPMSTRNCFRTQPQQQGSQAQHGPGFQSFYPIVTPPGSGAVYPQPQPTPRQPHGLSNNIPPPTNPTPPRPADYYCPPQYRLQRVSCRPHVEINLDKIGSSNKIYYYKTRYKEIGEKRREENKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>E3L018</accession>
    <name>E3L018_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_16193</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <dbReference type="STRING" id="418459.E3L018"/>
    <dbReference type="GeneID" id="10545720"/>
    <dbReference type="KEGG" id="pgr:PGTG_16193"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16193"/>
    <dbReference type="eggNOG" id="KOG1471">
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    <dbReference type="InParanoid" id="E3L018"/>
    <dbReference type="OrthoDB" id="1434354at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008526">
        <property type="term" value="F:phosphatidylinositol transfer activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0006892">
        <property type="term" value="P:post-Golgi vesicle-mediated transport"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
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    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.20">
        <property type="entry name" value="N-terminal domain of phosphatidylinositol transfer protein sec14p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001251">
        <property type="entry name" value="CRAL-TRIO_dom"/>
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    <dbReference type="InterPro" id="IPR036865">
        <property type="entry name" value="CRAL-TRIO_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036273">
        <property type="entry name" value="CRAL/TRIO_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051026">
        <property type="entry name" value="PI/PC_transfer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45657:SF1">
        <property type="entry name" value="CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00650">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00516">
        <property type="entry name" value="SEC14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52087">
        <property type="entry name" value="CRAL/TRIO domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46938">
        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="109"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="164"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="175"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP89905.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="358" mass="39945" checksum="DAF1FF5A20B04F97" modified="2012-04-18" version="2">MGKAISSDKGDQTAVQPARVRLHAIGHEPVKTSVCHQTGARQLLFQPARDPSSSQSQETLTPNQIEILKAFRSELEEEGHLKPSETLGTDDETLIMNSRFLKARKFDLGRPVNIQMFGSLDLSKLYSVIDKQSHFKVLVANCEALTREILPACSHRNQMINLQNSSQSDHDHHSQANSSSSHSSASPKITNAFCIVDLKGFTLTQLWQIKNIARTCFSISQDYYPETMGYLAIINAPKSFATIFKAVTPWLSKETISKINILGEDYKATLLEHIDDGNLPSFLGEKCQCDNQFSCSKNDANFDRSPWLKERNWKSQSWKKFNLQTPAQPNCLAIQSLIFLTGATPTTVALGTLSSRLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3L250</accession>
    <name>E3L250_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Protein CPL1-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_16651</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178335">
        <property type="protein sequence ID" value="EFP90625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335044.1">
        <property type="nucleotide sequence ID" value="XM_003334996.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L250"/>
    <dbReference type="GeneID" id="10530525"/>
    <dbReference type="KEGG" id="pgr:PGTG_16651"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16651"/>
    <dbReference type="HOGENOM" id="CLU_150190_0_0_1"/>
    <dbReference type="InParanoid" id="E3L250"/>
    <dbReference type="OMA" id="DSGACKI"/>
    <dbReference type="OrthoDB" id="439917at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048661">
        <property type="entry name" value="CPL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038955">
        <property type="entry name" value="PriA/CPL1_fungi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35192:SF2">
        <property type="entry name" value="APPLE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35192">
        <property type="entry name" value="PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21671">
        <property type="entry name" value="CPL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174322" description="Protein CPL1-like domain-containing protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="domain" description="Protein CPL1-like" evidence="2">
        <location>
            <begin position="82"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP90625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="144" mass="15672" checksum="A798F71F118BF9B0" modified="2011-01-11" version="1">MKNLTRIVVLAVAMGLASASQLSSSTALTRRTRQSFNLREIEIQLERQGCPAPLRACPILRSDISLDLNVALPKRTISKRNWECFNLQTDVNSCGSCDNDCMEIPNVARAKCDSGSCKIVSCRSGLQPKATVNHVTGSKSMKCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3L277</accession>
    <name>E3L277_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16678</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178335">
        <property type="protein sequence ID" value="EFP90652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335071.1">
        <property type="nucleotide sequence ID" value="XM_003335023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L277"/>
    <dbReference type="GeneID" id="10530552"/>
    <dbReference type="KEGG" id="pgr:PGTG_16678"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16678"/>
    <dbReference type="HOGENOM" id="CLU_611300_0_0_1"/>
    <dbReference type="InParanoid" id="E3L277"/>
    <dbReference type="OrthoDB" id="2497483at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="448" mass="50960" checksum="A16704C2959C66CB" modified="2011-01-11" version="1">MSRTRMALAPDPMDNSGYIGRFEAATGAEFPSHRAKRVRYEDPVFFPSDGPTTTAIHLQQPDSEWTPVNRQEQDQTLSHGITRRSSPPGETSGRPKRARDESQEIGPSVVGKQDKLSRPFGSFSEQAFEKILKAHNSRSFPYLKLADISPASVQLDSSIRLGQPIMGDYSQEINAYTGRYEVLIEDILETAHKLPNAYVGRKTRGWPATVVRPRHAEIEGEIILVLPTKDGDWRDAKSLVKQFKSLHRWIVYVHHLLLKNNNNPTSSSLQASYHQEMLLWLFGEVFNPKVGLPVIGRLDQASSNRQVFGPPQRWIMEFLVDKKTACTVSLALLGIWYLNAKSEEWQKEFRTDENFWSRMTTLINNDAKAGTEATGIHLRLKKRKKYEVKRNSNNMVEPQPRKTIAQIDNFRLLTPVQPDSKDTQVGLDKKSSELPGKVLKIRILSIEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3L293</accession>
    <name>E3L293_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAM50A/XAP5 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_16694</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178335">
        <property type="protein sequence ID" value="EFP90668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335087.1">
        <property type="nucleotide sequence ID" value="XM_003335039.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L293"/>
    <dbReference type="FunCoup" id="E3L293">
        <property type="interactions" value="338"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L293"/>
    <dbReference type="GeneID" id="10530568"/>
    <dbReference type="KEGG" id="pgr:PGTG_16694"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16694"/>
    <dbReference type="eggNOG" id="KOG2894">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037985_1_0_1"/>
    <dbReference type="InParanoid" id="E3L293"/>
    <dbReference type="OMA" id="DFIWVFL"/>
    <dbReference type="OrthoDB" id="1562195at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006325">
        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048337">
        <property type="entry name" value="FAM50A/XAP5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007005">
        <property type="entry name" value="XAP5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12722:SF0">
        <property type="entry name" value="PROTEIN FAM50A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12722">
        <property type="entry name" value="XAP-5 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04921">
        <property type="entry name" value="XAP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAM50A/XAP5 C-terminal" evidence="2">
        <location>
            <begin position="181"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="128"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP90668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="320" mass="37001" checksum="E7740601A4583483" modified="2011-01-11" version="1">MASSTSEKHRIAVHEKKRKEMMDEFERQKVEMTRETDRNRTGADRFVGKSDSMEESLKMQTVGLVKLEDFQQKRQALEEEKLREAARSNELKEDEPKKKKKKKSKYNLSFAMDDEDEGAGGIDKASADKQSTSSSKKGQFGKNPAVDTSFLPDRDREELDRKEREELRQKWLKMQESIKQEDIEITYSYWDGTGHRKEVTCKKGDSIAAFLEKARGQWPELRGVSVADILYVKEDLIIPHHHTFYDFIVNKARGKSGPLFNFDAHDDVRLVADATVEKDESHAGKVVTRAWYNRSKHIFPASRWEPYVPGKDYGAYKIKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3L341</accession>
    <name>E3L341_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178339">
        <property type="protein sequence ID" value="EFP90966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335385.1">
        <property type="nucleotide sequence ID" value="XM_003335337.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L341"/>
    <dbReference type="GeneID" id="10528381"/>
    <dbReference type="KEGG" id="pgr:PGTG_17238"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17238"/>
    <dbReference type="HOGENOM" id="CLU_398026_0_0_1"/>
    <dbReference type="InParanoid" id="E3L341"/>
    <dbReference type="OrthoDB" id="2510732at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="567"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="197"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="230"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="573"/>
            <end position="582"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="584"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="599"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="692" mass="76777" checksum="FB33E550C371CC2D" modified="2011-01-11" version="1">MTNNTHSPSNNSHTTTNHSPSPPPPTTEEPTEQPKPSEPEKPSEPSKPEKTKTNYTQQLCQQIHQDINHFNQSLNSLTTTTTPATTTTTETETETTIIHQIIKLIQANNQHTLSLFQSSVQTIFSEVLDSVHTHFSLDRNQQQLIEIDRQIIANAQNSDQETRERLQAILIQQKHQLTKQITELQSHLTHSINKTHQQQQQQQQQQPSAAASSSSSSSSSINPNIHPNLQQEQEQTHPSSSSHPPQNSINSPTPTRTSSALLLTTKRKRAEQPTSTIEYDEALLDEFSIPDHLSYSQYLKRSQHRTLNLHHSISTNPTQPPISNDISVLPITPSSNNNNSPTGALNGIPSNSDPALTPPSFLPNPALLENYFKPCSSGSKSSRRQSAILRNLVSAALGKLISHVTNNQQMHWPGSDTEERLAAIGLQIELLGGSKLTTEVIHKGPKNLQLQQCKRILLELELGLIRLKQIKAVPRPLRGAKKKFKPLDSPQLSLVHPLHQSQLDNQTAATPISVPDILAIAPSSSSSSAELAYTNTAPSLAEQHSPNVLGKNFENCDPALLSLSPAFPGLVPSDQSGAQPTGSEEPRSTDHRDLVHRYMSSNVHPNNNPKHSYIDDFPNGDHEAFMDCDDDQEPLHHHQQQHSHQNHEQNQNQNHEGQSDVDLGVQESDLDDPADRHLHQNESQLRRKLLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3L4V5</accession>
    <name>E3L4V5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17634</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178349">
        <property type="protein sequence ID" value="EFP91580.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335999.2">
        <property type="nucleotide sequence ID" value="XM_003335951.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L4V5"/>
    <dbReference type="GeneID" id="10531517"/>
    <dbReference type="KEGG" id="pgr:PGTG_17634"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17634"/>
    <dbReference type="HOGENOM" id="CLU_102646_0_0_1"/>
    <dbReference type="InParanoid" id="E3L4V5"/>
    <dbReference type="OrthoDB" id="2511357at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="174"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="176"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91580.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="205" mass="22894" checksum="5448DB131F156DB4" modified="2012-04-18" version="1">MSSICGASDNTIHITSVISSPEIVHHSQYSELSKFYIILQSNSSEPPPFIHEPETEIFFTGHLIASLFGSYPVIKVGAGKWVFHRGQHYELVEEFSKARVWGSGYVTQVKHNRSAPRRSYLTVLDPTPTSGACIRLLAWDSEILGQMSRIKRGNWIKFSGKAGIDAYKDSHIAETTDGQNQPNISSGLIPKPFESYPWTNGPRRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>E3L502</accession>
    <name>E3L502_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transporter domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_17681</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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    <dbReference type="KEGG" id="pgr:PGTG_17681"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17681"/>
    <dbReference type="eggNOG" id="KOG0065">
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    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
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        </location>
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        </location>
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            <end position="126"/>
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    </feature>
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            <end position="162"/>
        </location>
    </feature>
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        </source>
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            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3L590</accession>
    <name>E3L590_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_17452</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178351">
        <property type="protein sequence ID" value="EFP91715.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003336086.2"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17452"/>
    <dbReference type="HOGENOM" id="CLU_1315964_0_0_1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172469" evidence="2">
        <location>
            <begin position="21"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP91715.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="209" mass="22423" checksum="D57E713765652426" modified="2012-04-18" version="2">MRAFIYSALLTLVQLHLVLAAIDKVIIDLDPPTAVDLDPPTIGDPVSERSTEPEKSSPVPLAESRLDNLPPLSCEKEEPLRVSPSPLPPLSFFPTSADSASKKTHISPEKPTSSGTVLPATENHLGDCGYSSKKRKSLRMEQPASQGEAANSLYYSSSEDAAHVGLILSINPQSCEKVILAGPSRSTIEEQERNGKLGLSKLQAPNMTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3L693</accession>
    <name>E3L693_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18158</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178357">
        <property type="protein sequence ID" value="EFP92068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336487.1">
        <property type="nucleotide sequence ID" value="XM_003336439.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L693"/>
    <dbReference type="GeneID" id="10532025"/>
    <dbReference type="KEGG" id="pgr:PGTG_18158"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18158"/>
    <dbReference type="HOGENOM" id="CLU_148838_0_0_1"/>
    <dbReference type="InParanoid" id="E3L693"/>
    <dbReference type="OrthoDB" id="10335537at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174232" evidence="1">
        <location>
            <begin position="29"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="110" mass="12321" checksum="7C22EC9845B8F3A5" modified="2011-01-11" version="1">MQNLTLTTRLGLALALLMCILVAAPCSSKPFGQREIRARICNKCAEKYLNEGQVLKEKTLKLAIDPTKPDEEEIKKPCWGTGHVCTSMLRVKLLQCNQCHSFACTCPQRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3L6L5</accession>
    <name>E3L6L5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18087</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178360">
        <property type="protein sequence ID" value="EFP92190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336609.1">
        <property type="nucleotide sequence ID" value="XM_003336561.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L6L5"/>
    <dbReference type="GeneID" id="10537518"/>
    <dbReference type="KEGG" id="pgr:PGTG_18087"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18087"/>
    <dbReference type="HOGENOM" id="CLU_1611601_0_0_1"/>
    <dbReference type="InParanoid" id="E3L6L5"/>
    <dbReference type="OrthoDB" id="76215at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46929">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46929:SF3">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="165" mass="18523" checksum="A59A68FD2D8C1E28" modified="2011-01-11" version="1">MKRVIGYHADELATPGGSRKIETSEAIKKIEKFGKFNHTKWSRDDYEIVPDKFSNAFRPRGRLSTASGFGWNKAELMVTALEDFWNVFLVSHPASKKFKNKPFPEYSNHQIIFKGHTTQGDMQQLLGMEIADENSSTVPGDTGNLPETSDSTRAPAQHSGVRPPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3L6X0</accession>
    <name>E3L6X0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_17969</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178362">
        <property type="protein sequence ID" value="EFP92295.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336714.2">
        <property type="nucleotide sequence ID" value="XM_003336666.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L6X0"/>
    <dbReference type="GeneID" id="10537788"/>
    <dbReference type="KEGG" id="pgr:PGTG_17969"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17969"/>
    <dbReference type="HOGENOM" id="CLU_029800_0_0_1"/>
    <dbReference type="InParanoid" id="E3L6X0"/>
    <dbReference type="OrthoDB" id="2496402at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP92295.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="392" mass="43886" checksum="98F0773EDC8F19EE" modified="2012-04-18" version="2">MSKILDLPEELFEVIFTLLITTPTRWAHALHRASFVACTAARLRLVCRQWADWLYQHHLYRTLTFDSASRSLAFIESLRGRSRLLPRAKCQYLEVDFLWPSQNPPRSGQRDMITSDILESLVGLFSDTILTLDLKFVDFFTLPTRTIEAIGRIESLDSLQLDLSRWPSGDLGLPPPDPICFNSLLLEAQGLKSLHLALPVFLPTLEPGRMVGTSQYPAITHLDVEVTCLSPDVILGLSIALKPSLKLLSINDFGRSTDARLLLPVYETLRGTIEGLSVTSTSSLTPILDLTFPKLRVFAVHDSRDSLSDLLIRHLFSHAPIEAIAISSDSAEGSKPPFALDPFANLPQLQKLVFMNTPCDYSPAPMYLNACQVHHVRCIYLDHNDVSLIMKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3L7B5</accession>
    <name>E3L7B5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_18265</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178364">
        <property type="protein sequence ID" value="EFP92440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336859.1">
        <property type="nucleotide sequence ID" value="XM_003336811.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L7B5"/>
    <dbReference type="STRING" id="418459.E3L7B5"/>
    <dbReference type="GeneID" id="10538265"/>
    <dbReference type="KEGG" id="pgr:PGTG_18265"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18265"/>
    <dbReference type="HOGENOM" id="CLU_785586_0_0_1"/>
    <dbReference type="InParanoid" id="E3L7B5"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04818">
        <property type="entry name" value="PA_subtilisin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046450">
        <property type="entry name" value="PA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003137">
        <property type="entry name" value="PA_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02225">
        <property type="entry name" value="PA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52025">
        <property type="entry name" value="PA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PA" evidence="2">
        <location>
            <begin position="150"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="287"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="229"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="246"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP92440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="353" mass="38515" checksum="70429BEA12BDEB23" modified="2011-01-11" version="1">MPSGSDAFVLVKNLTGLTFSLKKRFDQDSYEIVKGKRLFSSFGSYELAPKTQVVLSDPYAVKVLKPQSESEKLSPRDRDHLTGIRVYYEDSSDWPSNRDSSSLEEEEGELDSISRPVMLATFGPDVRIPGSTGLSIGNGALEVVGLNPPNEYGCEPITQEPVVVHKKERPEDVNGKVVLVKRGQCTFADKVQNALLAGAKALIVANQDDTLLIPTSDRIHTRDPKEDLSSTSSSASDSAADDQESSSESNHRSSLGSDGDQDLLNLKLQSIPLLFCTLETGRSIGQLLANRSSPPPPTPSSSQHPRSKKLLLEIVGNPLHLLDHHISQTSTMTHPQFVVINGYKLANLQLKFV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3L7Z1</accession>
    <name>E3L7Z1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_18844</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178369">
        <property type="protein sequence ID" value="EFP92666.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337085.2">
        <property type="nucleotide sequence ID" value="XM_003337037.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L7Z1"/>
    <dbReference type="GeneID" id="10538994"/>
    <dbReference type="KEGG" id="pgr:PGTG_18844"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18844"/>
    <dbReference type="HOGENOM" id="CLU_061626_1_0_1"/>
    <dbReference type="InParanoid" id="E3L7Z1"/>
    <dbReference type="OrthoDB" id="10306342at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24383:SF20">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24383">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="242"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="11"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="97"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="254"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP92666.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="385" mass="44174" checksum="8F9217CEE594EF5E" modified="2012-04-18" version="2">MRHKHQRPHKSSQQTGQSWSTNQHQSAKTPRKQAVKYVVYPYKQDQRLLEDSQGEHYTVERLDHEETSAVDYPSNSAKDHLSLEPPSDTMFEDKEDDCNSKNSKNEKHKLDQIDLDQIEWDNRSDLDINAAPYQVQPEQSSTFKAFLEDGVVHFFPESNSTQQSEPDFDLKNYRAEDVDNWASTLLADEFEHLCVMGTNARTKSFQQYAISNLNQLTQTLTQQSSSTHEHLEHLVSTSNGCQDYRNYHHHQPDDISSNNFDQQRLASPDDESRSAFYNHSGNCNSHLEAFDDIGFDDSTFDNGGFDNDDFEDGGFNNGGFDNGGFDDCGFDDGCWNFDSSLLHLFHFSILQYLFLTIIPLSTNNYIVCLVASHEFKHQILVVKEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3L807</accession>
    <name>E3L807_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18197</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178370">
        <property type="protein sequence ID" value="EFP92682.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L807"/>
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    <dbReference type="KEGG" id="pgr:PGTG_18197"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18197"/>
    <dbReference type="HOGENOM" id="CLU_2237904_0_0_1"/>
    <dbReference type="InParanoid" id="E3L807"/>
    <dbReference type="OrthoDB" id="10518721at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92682.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="105" mass="11847" checksum="0BB73D5297D5D232" modified="2012-04-18" version="2">MVGPPTEDGQTTDRHTLQGDLKTLDHRRSASQNAWYDARSVFGWANIGIARRCTVRGKHRLIFRTNILDPKAKKKQTNIHDQAVEIVCLCGSPNSLFGTFVRVEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178370">
        <property type="protein sequence ID" value="EFP92692.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003337063.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L817"/>
    <dbReference type="GeneID" id="10539189"/>
    <dbReference type="KEGG" id="pgr:PGTG_18207"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18207"/>
    <dbReference type="HOGENOM" id="CLU_2623162_0_0_1"/>
    <dbReference type="InParanoid" id="E3L817"/>
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            <begin position="1"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92692.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="78" mass="8271" checksum="7B8C8ACB3959B76A" modified="2012-04-18" version="2">MTSLSVTAWPSPGVDEGLSQLSCPGNALTKQPRRKNDVNFVNEFPVRLFVAQVTMAHGGYIPDLAITACNIGLAGAVR</sequence>
</entry>
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    <accession>E3L8F0</accession>
    <name>E3L8F0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    <gene>
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    <organism evidence="2 3">
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        <lineage>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178374">
        <property type="protein sequence ID" value="EFP92825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003337196.1"/>
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    <dbReference type="AlphaFoldDB" id="E3L8F0"/>
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    <dbReference type="KEGG" id="pgr:PGTG_18889"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18889"/>
    <dbReference type="HOGENOM" id="CLU_044243_2_1_1"/>
    <dbReference type="InParanoid" id="E3L8F0"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="180" mass="20579" checksum="42D86FA201042408" modified="2011-01-11" version="1">MTTIIRKETPATSQDKETSKEKQPLNKEKSSAMEIDEIDPECSEVTPTRPSTPEIRILSKEDQVKLRVKEHVGLWKQCLVASREGPTARLRALLTTAQESQKALQKLIDNEEIESYVKGWNPWEEKKIHFPSPPKKNLMKFKRNEPGKKQSSSSTNFSDPAKWKKVTDLLQMASGLYQNV</sequence>
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<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8Q6</accession>
    <name>E3L8Q6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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        <lineage>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178376">
        <property type="protein sequence ID" value="EFP92905.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337324.2">
        <property type="nucleotide sequence ID" value="XM_003337276.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L8Q6"/>
    <dbReference type="GeneID" id="10542714"/>
    <dbReference type="KEGG" id="pgr:PGTG_19023"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19023"/>
    <dbReference type="HOGENOM" id="CLU_033090_1_2_1"/>
    <dbReference type="InParanoid" id="E3L8Q6"/>
    <dbReference type="OrthoDB" id="10276938at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP92905.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="477" mass="54612" checksum="41DA6F3329BC2D79" modified="2012-04-18" version="2">MLNHEPNQNDIIDQIVKGLSILLTKWNCRDLLAPKSSLPLNADDSTRDHDDVRLGRWKGLSPDKIKDQKALLLQLETRILPSLKHQLADLLKSLDLADPSKEPRPNLLDVLEIVSQIGQSFNQINGFLYSIAGNRNSSEHDHDYGGVKYFRYRPLMEKINRLMSHHIWILFQRHIPGILKRSKDHPIWLNDRSDLITHREQLIEGIARSSDYIDTIIKSSKRSDFNILQDEWRNYSNDLNNLLKSITVKINPADEEECLLIDVGRPESKYYKATVDQNHSINTDSAEMDFNSNNHEDTQSDANSLIDHEDNQSDTNCSTCSSEESFLRFMKLTQSIIPVIKLVRILLNKLSNPISRIPFTIDSKMSSMDIQSVQSNLSSLNYTIRSILNSLHYGYHSDQIMDQLENVKMLQERLSKDFDSSIALLCLYLVPSTDIELDLPASGDLIKTWSLGLSEQFSLATKIFDKALSEFEKESNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8T8</accession>
    <name>E3L8T8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mediator of RNA polymerase II transcription subunit 19</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Mediator complex subunit 19</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_18976</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the Mediator complex subunit 19 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178377">
        <property type="protein sequence ID" value="EFP92963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337382.1">
        <property type="nucleotide sequence ID" value="XM_003337334.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L8T8"/>
    <dbReference type="STRING" id="418459.E3L8T8"/>
    <dbReference type="GeneID" id="10542855"/>
    <dbReference type="KEGG" id="pgr:PGTG_18976"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18976"/>
    <dbReference type="eggNOG" id="ENOG502R766">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_771932_0_0_1"/>
    <dbReference type="InParanoid" id="E3L8T8"/>
    <dbReference type="OMA" id="GIFCRED"/>
    <dbReference type="OrthoDB" id="2160599at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070847">
        <property type="term" value="C:core mediator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013942">
        <property type="entry name" value="Mediator_Med19_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28270">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28270:SF1">
        <property type="entry name" value="MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0010">Activator</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="102"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="246"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="23"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="251"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="279"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="300"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP92963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="375" mass="41261" checksum="24DA95E79D140ADB" modified="2011-01-11" version="1">MQNLQPNSSALHTQDHYHHHQKQQQQQHPSQETTSTPQAGTSSTSNRQLHPNPKTLYFPVIDQGHLPAAPISGSHDLLSLFNVSSMYDWFVKPYLKPLDQTNKLGPTNNHHHHHFPNTNGDLKGKGKADIDLTGLSPAQLENLGQKRIKMEKSYGHFVADVGGRNSIKKDHQLETWISDPNFQEASVPNFLKPFSNEALSQAFTLQPGILPGFDSSVWVAEEGGPKKKRKKRKQGEMLAGSNSTLAAAHLQQPHQSPTQQTTMTTTTTTTTTSGTPTQYAVSSTTQAQPTRPLRASMVDPSSHLNSHPHSSHHPAAQASHLHLHQNQQQQHNSPGMFCREDDYRKKKRVKIGNPAAGKTSGSGSSGPNHQVALQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3L991</accession>
    <name>E3L991_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19221</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
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    </feature>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP93116.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
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    </evidence>
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
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        </location>
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        <location>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="125" mass="14117" checksum="EFA147537D164E87" modified="2011-01-11" version="1">MRFSILITLVALLLIQSEAAHSKFVCNDGSKKDAKYGICVRKIVEQIDSHDQSLPPLKNDQLLVIAASPMGNGFTCAKLDIAKRPVEARYCCDFPPEQHKVLPHTKKYIDDHCYTRDAKRKPHRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3L9H7</accession>
    <name>E3L9H7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="7" type="ORF">PGTG_19441</name>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
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            </authorList>
            <dbReference type="PubMed" id="21536894"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the THADA family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ARM-like"/>
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        <property type="entry name" value="ARM-type_fold"/>
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        <property type="entry name" value="THADA-like_TPR_C"/>
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        <property type="entry name" value="THADA/TRM732_DUF2428"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051954">
        <property type="entry name" value="tRNA_methyltransferase_THADA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387:SF0">
        <property type="entry name" value="DUF2428 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14387">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10350">
        <property type="entry name" value="DUF2428"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="TPR_Trm732"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF25151">
        <property type="entry name" value="TPR_Trm732_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA (32-2'-O)-methyltransferase regulator THADA-like TPR repeats region" evidence="5">
        <location>
            <begin position="186"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2428" evidence="4">
        <location>
            <begin position="651"/>
            <end position="913"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA (32-2'-O)-methyltransferase regulator THADA-like C-terminal TPR repeats region" evidence="6">
        <location>
            <begin position="916"/>
            <end position="1073"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="26"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF25150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF25151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP93202.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1633" mass="183245" checksum="4F9F3004E0AB7F98" modified="2012-04-18" version="2">MRKSPWHSRSSISSSGKLKSTKRNHLQGNQAANPNPSILPPENLRQISQAIWAHWNAPSPTISYKIKNALKNLFEYMNHSRINLPQSSSVQFFLESVKQSILLEKRSLSILSYLLPHLSLEDALQAFQLFAAGPAEFLGTILGSIGDDKLASVIGQLAVHWTTKVEGWDSKDSKTTADSANLSSDWISTVFSILFSSEERKRQYLCQYYLLPLFKTRPVCFMELAERLSLISGPRFQKISIAKNAFDHLSGIIALARLNPRQSGPHQKDRNHVDPWLPDDLLKVALSHPSVSLRSSALAIICQSTAPTSPLPLPHLDLIFQFFVWNFGEVEAELKQNIFSNLNALFNRLRDSSTSSNKKLGELINKKSNLNQSNNHVGAIETTTAVNGLEATQLQILIVEAEGYLQAVKTFIEKFMRLCRSNLTISSPYRCQMASLTYLQILLNTGLDPRYQPLTKSSLPSLHHNDQASLLKKPSSSFPFEIRVVDRALFHKLILSLTSTYVDVREAAFTLLEKTGCSALDSPAEQGSQSVIRFMPFAVQCCNSSREAENSTAALLFRLVLKSSLIHNFELPSFLLPRNNNHSETTNPLAIFLVGRLDDLEDRVLQSELNLGKACEERPMAGSIMLMSELFKCLSKDMIGSLVASNDLIVIMNRTRKLILRVWSSSAIVLCQSSDRQSDVVPDHEEARAFEFISTEEDGDELEDDADRNPGNRHKTILSACWRSMKEASALLMQTVKLSLIAEEQSAGDNRNTFLSYQDLTEIGELFEQWLLEIRHRGAFGAIHASYSGLCDALCALPKESASSQLPVLWLQAHISAITSRKISTTRRSAGMPYCILSACQALSRSNPNQLRESLSTILSLAESQEISSEVQVHILNTLKILLTDGKVSAQFSSVLIERSYNLAIKTFVSPDWRVRNGALILFSGLTNRVFGTRSLTLDRSYSNLCKRESLSDFSRRLPTMPSILLQELRRSMEAGIHLVSTSHSHGPMFAVLTLLALLQNPDDALTATEFIPLVRACLLSKVSKVRSIGADAMTGLVPCSQVPGLICDLLDQAVKSHCHNEVHGLLLLINRLIQLPSALLRDQKSNIEVALARSAPKFLHSMTIPLVSQSTFLEILESLESRFMIKVDDHDCLFSPGMMTDKIQELRRIRAPALSSFETQATRNVLGRNQARETVLKLLEVGSTSTRVTVFRFLEKEENRETLKDPEIIDAVFRIGRQSTHPISVRLPALKLLTTIGSGLKTTLWPVEELFNECNSTLAIPMRDALLVLSACFFVNSLVDRNSKIDSSFVRAWADKILNKIQQAAHEDQSTETRLSAVQGLTEFMKVFKRNGVLSTSVKLRILELLISLIQDDDDEIRSSLIKSLHQTKFFSSLVDSQPNQEDQHCFQLELVPNLILEKLLHLAIGLNAFFGFKTLLNRDNFQADLDQLLQPPDDLFITERLNLYKDDLMEFELIQTITSKRLIQLDKLDDDDQQQPNERLGTQLNCKKIDADFQDLVDLNPELRTFKYLLSFLVPKSIKTLKGFEIFGDDLFINHLNRNPGPNADDHDAHFNVISFKAWFSILLRLFNSITIFNRFPPNIQSSILASISDLPPSFPVPQGHLPHQDPVLDALALEIDHQFTLIQNFLTAFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3L9J5</accession>
    <name>E3L9J5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19459</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178385">
        <property type="protein sequence ID" value="EFP93220.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337639.2">
        <property type="nucleotide sequence ID" value="XM_003337591.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L9J5"/>
    <dbReference type="STRING" id="418459.E3L9J5"/>
    <dbReference type="GeneID" id="10543371"/>
    <dbReference type="KEGG" id="pgr:PGTG_19459"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19459"/>
    <dbReference type="HOGENOM" id="CLU_1781965_0_0_1"/>
    <dbReference type="InParanoid" id="E3L9J5"/>
    <dbReference type="OrthoDB" id="10549371at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="122"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93220.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="146" mass="16148" checksum="C65D0284B9BEBF65" modified="2012-04-18" version="2" fragment="single">PFFLILSPQYAGYDLETHYNQLSRYLDEAMGGGGGRGGGGALENGKEWRRTMEHPMNWRLMLPKRRKKNKGKKNLPPRQETNRQTRWLAPATNGGPKSLAPRRQCATPKQRGSRGGSKGCTQPPSASNPQPPSASNQVQTNHEGAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3L9K0</accession>
    <name>E3L9K0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_19171</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178386">
        <property type="protein sequence ID" value="EFP93225.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337644.2">
        <property type="nucleotide sequence ID" value="XM_003337596.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L9K0"/>
    <dbReference type="GeneID" id="10543376"/>
    <dbReference type="KEGG" id="pgr:PGTG_19171"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19171"/>
    <dbReference type="HOGENOM" id="CLU_2868686_0_0_1"/>
    <dbReference type="InParanoid" id="E3L9K0"/>
    <dbReference type="OrthoDB" id="10457591at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP93225.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="64" mass="6929" checksum="D84B2E919CCDA07F" modified="2012-04-18" version="2">MVVRREVGLACPSRSFRPSTARTSQFDDSSDGYVRELLGQAGPMTCWTGMSEQFAEAVSSGRPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3LA41</accession>
    <name>E3LA41_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Catalase immune-responsive domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19493</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178392">
        <property type="protein sequence ID" value="EFP93416.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337835.2">
        <property type="nucleotide sequence ID" value="XM_003337787.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LA41"/>
    <dbReference type="STRING" id="418459.E3LA41"/>
    <dbReference type="GeneID" id="10537283"/>
    <dbReference type="KEGG" id="pgr:PGTG_19493"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19493"/>
    <dbReference type="HOGENOM" id="CLU_1982642_0_0_1"/>
    <dbReference type="InParanoid" id="E3LA41"/>
    <dbReference type="OrthoDB" id="2512064at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1370.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010582">
        <property type="entry name" value="Catalase_immune_responsive"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020835">
        <property type="entry name" value="Catalase_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06628">
        <property type="entry name" value="Catalase-rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56634">
        <property type="entry name" value="Heme-dependent catalase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Catalase immune-responsive" evidence="1">
        <location>
            <begin position="29"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93416.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="126" mass="14011" checksum="D1070CC5E78B6276" modified="2012-04-18" version="2">MPRMIFSYHAQIELGIKYTQLPVNKSTLDFKPARALWSKVFEDGAKEYFVKNVSGHLGNVSLDRIKTDQVAIFLAVDKDSGNRVAKAISLKDTPEPYKPEPPAQATKFAPNLKSSGQMIFENSIPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3LBB0</accession>
    <name>E3LBB0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ser-Thr-rich glycosyl-phosphatidyl-inositol-anchored membrane family-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_19870</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178411">
        <property type="protein sequence ID" value="EFP93835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338254.1">
        <property type="nucleotide sequence ID" value="XM_003338206.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LBB0"/>
    <dbReference type="STRING" id="418459.E3LBB0"/>
    <dbReference type="GeneID" id="10535286"/>
    <dbReference type="KEGG" id="pgr:PGTG_19870"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19870"/>
    <dbReference type="eggNOG" id="ENOG502S56Q">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078127_3_0_1"/>
    <dbReference type="InParanoid" id="E3LBB0"/>
    <dbReference type="OMA" id="IYFFQFT"/>
    <dbReference type="OrthoDB" id="2432613at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052982">
        <property type="entry name" value="SRP1/TIP1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40633:SF1">
        <property type="entry name" value="GPI ANCHORED SERINE-THREONINE RICH PROTEIN (AFU_ORTHOLOGUE AFUA_1G03630)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40633">
        <property type="entry name" value="MATRIX PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_8G05410)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173281" description="Ser-Thr-rich glycosyl-phosphatidyl-inositol-anchored membrane family-domain-containing protein" evidence="2">
        <location>
            <begin position="17"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="116"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="146"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP93835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="270" mass="26833" checksum="C50D8DF9F3C35369" modified="2011-01-11" version="1">MISAVVLSLLVASASCATGVVPTFPGPGQAFKEGGDCPISWNLDTTGKWTNFSVDLMSGSNFAMQTVSNVFKDRDGTKGETTYTWKCPTVTPTSAIYFYQFTQAGADTTWTTRFAITSPSGESTPPANQTQPGGAQIPWGVGHLAGAGAEPAPASPATTAPSATANITALNTPSPATANATNSTTGALAGANNTSSVTGTPAATSPGSTSSGSSSSGSYSKNPASASPSNSRPTNPSSNTTSTPSAGAHTVTVCQNVAAVLTLAAVLIVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3LBC7</accession>
    <name>E3LBC7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_19706</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178412">
        <property type="protein sequence ID" value="EFP93852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338271.1">
        <property type="nucleotide sequence ID" value="XM_003338223.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LBC7"/>
    <dbReference type="STRING" id="418459.E3LBC7"/>
    <dbReference type="GeneID" id="10535303"/>
    <dbReference type="KEGG" id="pgr:PGTG_19706"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19706"/>
    <dbReference type="eggNOG" id="ENOG502RDJ5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033090_3_1_1"/>
    <dbReference type="InParanoid" id="E3LBC7"/>
    <dbReference type="OMA" id="IDRTIEW"/>
    <dbReference type="OrthoDB" id="2500854at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1410.10">
        <property type="entry name" value="I/LWEQ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="249"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="104"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="264"/>
            <end position="281"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP93852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="435" mass="48375" checksum="3EA591D547F1412D" modified="2011-01-11" version="1">MVVPQETSIASLANSFKSLQPSEKGQDMEKQKCLVVQAFRSLIAKCPLICLEPFSSSHEENLPGLFEQIELRAELLKRLQSGLLPSLRHRITALSLSLDPCDLRKNATGQLKQVLEILGELEQTLDQIKASVASIAPVLRPSLVSNDSHLKNLKAFRCVRVRLKVNDLAMLVSELFQTCVRFIENLTYSSSNEPRGRAEVLSLTSLSWNLIDTTIEWFDRSEFSLLQDQWQSEVGLVDDALDELTKLINHTGPEGESDSAANSSDEDGDHDHDDDDDDDDMSFGANDSRPSEHVIQLAKSAVPIIKLSRLFLNKLSKTTTQKEPFGLVTEMSSSQLESLLASTGSISRIVDEIVCTLSDADDDDHALATEDLIQSGNNLSEAFREIQLVLDRYLIPLISEEDSSSSSKVNIKTWFSEWKQAIPLATQNYIPNENS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3NXA4</accession>
    <name>E3NXA4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20103</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS989925">
        <property type="protein sequence ID" value="EFP94203.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338622.2">
        <property type="nucleotide sequence ID" value="XM_003338574.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NXA4"/>
    <dbReference type="STRING" id="418459.E3NXA4"/>
    <dbReference type="GeneID" id="10527290"/>
    <dbReference type="KEGG" id="pgr:PGTG_20103"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20103"/>
    <dbReference type="eggNOG" id="ENOG502S8TF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030585_0_0_1"/>
    <dbReference type="InParanoid" id="E3NXA4"/>
    <dbReference type="OrthoDB" id="5778525at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="256"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="365"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="176"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="305"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="377"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP94203.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="453" mass="49598" checksum="1E95FC61CA335C15" modified="2012-04-18" version="2">MSPASRDQLPSPHLTQDLSLNNFLHDALPNQQSNPSASIRPPPSNAPIESNSRTPNQIYSSNQYFHEPSCVSAHNLINYPPPNPPTPPPLFDASEQDLLSSFLNILGDSNGECDFDPQGMPEGMPVLGELKRRLEECGQDGPVPYFGKRETDEMGREVESRLKISEPEYSHMRRPSLQSFQTTSGSLSSSSDSHIYQEQGALSRKKAKTSNTPTLLDPLRPVHIPEVPHHHGVELEPDVEMHHAWNRNPCGNSGEWPSQLLLGHDPHARQPSYPHHGQTIDPIPTQAPSVSPVFPPDANGPLDGHPVETKDREDQQGSRRVSKRNGKPGKSTHMVSEQRRRNAIQGGFGSLVEILRAGEAQSGISIATPDLPSHPPGSAGSTTTASNTTSTNHNKKPKTRGRGRRGEIETGASKSVVLERAAEFVKWMADGNLALFNEINRVQSILQNHGIQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>H6QP39</accession>
    <name>H6QP39_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20753</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EHS63169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003890675.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QP39"/>
    <dbReference type="GeneID" id="13541501"/>
    <dbReference type="KEGG" id="pgr:PGTG_20753"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20753"/>
    <dbReference type="HOGENOM" id="CLU_2607144_0_0_1"/>
    <dbReference type="InParanoid" id="H6QP39"/>
    <dbReference type="OrthoDB" id="10277331at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="79" mass="8974" checksum="577C1868F74C5DC1" modified="2012-04-18" version="1">MVEVSLRDCDARRTQHLIKALSTDSRHVQQAQPELSKSQAQAKEKRITPTNPLIKKTPQIFKMIPIVKPILRMMNGQPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPJ4</accession>
    <name>H6QPJ4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20861</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EHS63879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890574.1">
        <property type="nucleotide sequence ID" value="XM_003890525.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPJ4"/>
    <dbReference type="GeneID" id="13543198"/>
    <dbReference type="KEGG" id="pgr:PGTG_20861"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20861"/>
    <dbReference type="HOGENOM" id="CLU_1971607_0_0_1"/>
    <dbReference type="InParanoid" id="H6QPJ4"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003605707" evidence="1">
        <location>
            <begin position="23"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="128" mass="11234" checksum="66C0945886255C93" modified="2012-04-18" version="1">MQFNTLCLSVSAAIFTLSTVLAVPTPTIGLGLGGGIGLGIGGGLGGGYGGGYGGGYGGGYGGGYGGGYGGGYGGGLGYGGGLGYGGGLGYGGGLGYGGGYGGGLGYGGGLGYGGGLGYGGGLGYGGGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPQ8</accession>
    <name>H6QPQ8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Crossover junction endonuclease MUS81</fullName>
            <ecNumber evidence="14">3.1.22.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PGTG_20787</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="18" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Interacts with EME1 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, D-loops, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication fork intermediates. May be required in meiosis for the repair of meiosis-specific double strand breaks subsequent to single-end invasion (SEI).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="14">Interacts with EME1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 14">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the XPF family.</text>
    </comment>
    <dbReference type="EC" id="3.1.22.-" evidence="14"/>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EHS64084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890493.1">
        <property type="nucleotide sequence ID" value="XM_003890444.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPQ8"/>
    <dbReference type="FunCoup" id="H6QPQ8">
        <property type="interactions" value="118"/>
    </dbReference>
    <dbReference type="STRING" id="418459.H6QPQ8"/>
    <dbReference type="GeneID" id="13542922"/>
    <dbReference type="KEGG" id="pgr:PGTG_20787"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20787"/>
    <dbReference type="eggNOG" id="KOG2379">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014329_1_0_1"/>
    <dbReference type="InParanoid" id="H6QPQ8"/>
    <dbReference type="OrthoDB" id="5963188at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048476">
        <property type="term" value="C:Holliday junction resolvase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048257">
        <property type="term" value="F:3'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000727">
        <property type="term" value="P:double-strand break repair via break-induced replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd21036">
        <property type="entry name" value="WH_MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20074">
        <property type="entry name" value="XPF_nuclease_Mus81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000371">
        <property type="entry name" value="Crossover junction endonuclease MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10130:FF:000003">
        <property type="entry name" value="Crossover junction endonuclease MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.110:FF:000001">
        <property type="entry name" value="Putative Crossover junction endonuclease MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.670">
        <property type="entry name" value="Crossover junction endonuclease EME1, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.110">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027421">
        <property type="entry name" value="DNA_pol_lamdba_lyase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042530">
        <property type="entry name" value="EME1/EME2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010996">
        <property type="entry name" value="HHH_MUS81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033309">
        <property type="entry name" value="Mus81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047417">
        <property type="entry name" value="WHD_MUS81"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047416">
        <property type="entry name" value="XPF_nuclease_Mus81"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13451">
        <property type="entry name" value="CLASS II CROSSOVER JUNCTION ENDONUCLEASE MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13451:SF0">
        <property type="entry name" value="CROSSOVER JUNCTION ENDONUCLEASE MUS81"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14716">
        <property type="entry name" value="HHH_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47802">
        <property type="entry name" value="DNA polymerase beta, N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10 14" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="11 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 14" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8 14" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13" id="KW-0469">Meiosis</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 14" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12 14" id="KW-0539">Nucleus</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ERCC4" evidence="16">
        <location>
            <begin position="597"/>
            <end position="701"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="84"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="273"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="488"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="488"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU369042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EHS64084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="899" mass="100200" checksum="29AA560AB2F5DCCD" modified="2012-04-18" version="1">MPPSKKYPNAEWAGWIQEVANQATENNAKVSQTYCKAAKAIKNHPIKLNHPRDALSLPGVGPKVVKIITDRLEIWCQENQVSFPSQVPLNKDGSDSGLSSDDATRPRPRPAELVDEEDHNPKPRSASNCQKSSAHKAHPTAPSKPRAKKPYQPKPRDPAWGILAAMYTICEPTDLKKFSSREIIAERAARYSDCTYVDLREDTSNRYGSHWQSGIKALIKYEFVITHATLRPAKYALTRTGYEFATVIAAQEDIPLLDVNMGPNTLCFEKSASSSVSRDPIPRETAKPSGNAHSEVSREVNFNEKSLPGNLGSETGNSLDAVTGAPSAFRFSYLNNANQRVLQLGEAATRLNSKTKQSFYKIEYPCAQQLHQFRRNHVLVDEKESCITLPRQETMIGWLKSEKSIPTCPGLCPTRAPRKPDLAKTDRASTVNFLLMSEKNSSKPASNIYKPPEILQAASEFAVAADAHPEPRLAKCPSQVPAPMITSVTSEHAQPQSSMVQPKQAQPKSNTNSSGSRPRASFATADHVPGGVPVVQPKQALPKPNVNSSGSRPRASFATADHVPRGVPKPRASIGTIGSIANQVRAEVWKARTYSVMLVIDNREVQSQNNRDGIFHACLSRARQTMGDQVKVQQRPLAVGDAIWIAVHNETGNEVVLDSVVERKRLDDLCKSIVDARFHEQKARLKNSGLKDRIYLVESYYSHNNRDLYEQQIQTSQVEIMLLDDCHLQSTADWKESVEYLVMRTNVLNQLHQTIDLSVIPDKHIDRNSYLSFINELRRIRPDDYWVTSYNVYESLNGKSGNLTVQELWGRMIHQISGMSVEKVGEFVTRWPTPRSFTDEFFHQYYRSEGAASSAHGAPKDCLSKWIEDQFCSGAAHSRKKLGPALSKKIAELFSLSSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPX2</accession>
    <name>H6QPX2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20922</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EHS64310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890466.1">
        <property type="nucleotide sequence ID" value="XM_003890417.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPX2"/>
    <dbReference type="GeneID" id="13542990"/>
    <dbReference type="KEGG" id="pgr:PGTG_20922"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20922"/>
    <dbReference type="HOGENOM" id="CLU_003292_3_2_1"/>
    <dbReference type="InParanoid" id="H6QPX2"/>
    <dbReference type="OrthoDB" id="3248529at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052055">
        <property type="entry name" value="Hepadnavirus_pol/RT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050:SF7">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="385"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="484"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="390"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="411"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1054" mass="119815" checksum="5E47A25AB4888785" modified="2012-04-18" version="1">MENTQQKITRSKTGTRKEADPASQTATQSATLNLPNAIDQGRSATKGPPSKEVPSKDILPHGGSAAELPSSDAHDSDGEESLDLITKVPQTVKEVIDTTSVPPEASVGDQRSHVWEKIKEAQTAGDAILTKILLAAYKDLDDLADTLTPPKITRSSSALPVLSTVESKTEIATSATVTELEDNLVYAVGTVTSHQDIGFTPYFDENIRKLKAPLPLTIFDREWQKKAIAAHLTIKPSKSSEDKAYRGLAYHDEWTQSHSSWTNNHRSFYITLQDVYNKKLFAEKLRIHKENCDEIADVYGFMTAFRYDMQTRMNAFAHRVPSKDGAAIPDISVKQMVVVEQCYSTVRSFGEASWKDNNYAPGFSHAGYDPDTGLRRPDFHKSASYQSTFNKEKQHQPNHQERRREKRWFNENAQHNTSNKPYGFENKHHYQNFNNNHNQFDYVNYNQHPYNTQHQHPYNTHPYNAPYQNNQGGSNPNRGFVGGSKRFKGANSGFESGGRGGGSKDEQDNNKQVIWPTGVKCEMDVPKWQLALKSAGLLPSLNHVLDGFKFGFDQGIPKHRLGTLRWFTPDNHSSAENSREKIQSSIKEEVSNGRMFGPFTHEEVARKYEFFRTSPLGSVVNADGKMRPINNLSFPKRNSETPSVNSFVNKLDFSTTWDDFKIVSEFFRNSESQYELALFDWAKAYRQIPTLESQWPFLMVKDLNGGLYIDTRITFGGVAGCGSFGIPADAWKKIMEHEFNVVKVFRWVDDNLFVKNLSSLCDMKSITARSVSLGVATSEEKSTMFANEQKFIGFVWNGIEKTVRLTDKKLEERKNQIAVFLEERATFTFNEAEVLAGRLNHVSLLLPQLRCYIRSVYRWMNQWKKRWANREIPQDVRDDLKFWTDTLNNYQHTRLCPLTTPIEIKWVGDASTSYGIGVLVGDNWAQLKLKPGWEQAAPPRSIAWLETVAIRIGILMLQVIKPDLKGNNFIVYTDNTTTQSTLRSRKSKDHHSNLEWKEIQRLLIEMELDITPKRVVSKENTADGLSRGVVAPHTPRNRVIFPIPADLEKFMYHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQK7</accession>
    <name>H6QQK7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21167</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EHS62659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890205.1">
        <property type="nucleotide sequence ID" value="XM_003890156.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQK7"/>
    <dbReference type="GeneID" id="13541285"/>
    <dbReference type="KEGG" id="pgr:PGTG_21167"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21167"/>
    <dbReference type="HOGENOM" id="CLU_003292_6_3_1"/>
    <dbReference type="InParanoid" id="H6QQK7"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="415"/>
            <end position="564"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="68"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="440"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="474"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="564" mass="62969" checksum="B133CE3203D52F76" modified="2012-04-18" version="1">MAIQTLCWAKLARGDRNGLCLSASSYRPFKPIFLPTEPESYQVGKAPFRRLSYPPSKRQKSRIIPTSERPSNMESTLRSAPKVTRAKSAKNPVVNNENTSSPSAETETPVNPTITLDASQDSGGDESVDLISKNPVLSTETLNEIASKTSVPPITEQIDEREHIWAKIKDAQTAGDEILARALLIAYESMDIQKPNAPARSTSAHPILVTANPGGQPSSVPTETQLEDNLIYAVGAVTSHQDIGFTPYFDENIKKLRAPIPLTIFDKDWQKKALAAHMLLKPAKSSEDKAYRGLAYYDEWTQTHSAWTNNHRSFFITLRDVYNKRLFAEKLLIHKANCDSIADVYGFMTAFRYDMQIRMNAFAHRLPSKDGAAIPDISVKQDIVIEQCYSIVRSHGESGWKDNFYAPGGSHAGFDPDTGLKRPDFSRSNSAPTPYGGRNSFINNNSHFSYSNHQGRRRENNRNRFEGNFNQENPIQHYNYNPGYNYNQHFNAQQHRGNFGHSDHQNQNHHGGHPYHQNNSQGGSQGGGSYSQSGSDSRKRFRSGKADQNERVGGNDVNASNPKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQY0</accession>
    <name>H6QQY0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21267</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EHS62917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890077.1">
        <property type="nucleotide sequence ID" value="XM_003890028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQY0"/>
    <dbReference type="GeneID" id="13540879"/>
    <dbReference type="KEGG" id="pgr:PGTG_21267"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21267"/>
    <dbReference type="eggNOG" id="ENOG502SARS">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013843_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQY0"/>
    <dbReference type="OrthoDB" id="2505264at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="352"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="406"/>
            <end position="461"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="489"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="533"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="606"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="648"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="683"/>
            <end position="693"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="693" mass="78196" checksum="D8F35FEED69A9061" modified="2012-04-18" version="1">MPNLKSFEVRMGTLTANEPSLRRQQQRQQRFVLGGTDFNEITPGLRSAQLIPCTIERLHLEGICFSPNLLILPQLFPNLKFIILKSLTWGRMIYELIRRSPGLEILKMIDFCFDQEMEEDLPSDWNFNCWEEDISETGSDEGGMTLKAPPINAMNLIELHLLGEGTPHIWSVLGECIQNPIIKMPKLEKLVCESLDLEEEEQALIDLADLAPNLREISIRNCILRDEVDLYHCIRCLPDLELLDCRLTENITSNLINALALSVPNIRHLDVRGCPYVHVTSVARLAETIRDSSDSERRIEYIGVDKPLEPTFALESAQEDSVKWEIWQLWQAWNWLDFTHVLLDQEEWKARNRGRKDKMPVRPDPPVHLPVHRPQELQQTSSHGPRIRLTCRSQQEGQRPGHSQEADEEEEEEIDEEEDEEEDEEIDEEDCEEEDEQGELEEEPVDEEEEDQQQQQEEEDSPSQQARQPRPGPFQNHHPQAHNRIPYPSHHPPSQPAPTPSAPVASSNHDRPAPAPPSVAPPAASSHTRESHQTAGQTSSLDPNLKQNLVENDSNSHRPHDPTIQTAAPHPIIKQDETSNGKLLPIESTNHHHHQKVDSNPGVNDSKSDLNELRSDESESQINKGVDGDGEARNGISANGSDSRTAHVPPPQSEHPLRPPSSSPSPAPLLPSSALPPPPPLLPRDLPQSLPPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRB2</accession>
    <name>H6QRB2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21433</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EHS63103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889964.1">
        <property type="nucleotide sequence ID" value="XM_003889915.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRB2"/>
    <dbReference type="GeneID" id="13540540"/>
    <dbReference type="KEGG" id="pgr:PGTG_21433"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21433"/>
    <dbReference type="HOGENOM" id="CLU_2672260_0_0_1"/>
    <dbReference type="InParanoid" id="H6QRB2"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="75" mass="8453" checksum="3B1BD2A73458FA27" modified="2012-04-18" version="1">MPSEEPIPNLGSSHSEHEGSHEENITDDGQDVKVAVFFTIYVNMPPRQGHRGMVRVPPRCFKHHKGCLIQMCQAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSI5</accession>
    <name>H6QSI5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetylglucosaminylphosphatidylinositol deacetylase</fullName>
            <ecNumber evidence="2">3.5.1.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_21798</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PIGL family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.89" evidence="2"/>
    <dbReference type="EMBL" id="DS178294">
        <property type="protein sequence ID" value="EHS63722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889544.1">
        <property type="nucleotide sequence ID" value="XM_003889495.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSI5"/>
    <dbReference type="STRING" id="418459.H6QSI5"/>
    <dbReference type="GeneID" id="13542790"/>
    <dbReference type="KEGG" id="pgr:PGTG_21798"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21798"/>
    <dbReference type="eggNOG" id="KOG3332">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2134790_0_0_1"/>
    <dbReference type="InParanoid" id="H6QSI5"/>
    <dbReference type="OrthoDB" id="440160at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000225">
        <property type="term" value="F:N-acetylglucosaminylphosphatidylinositol deacetylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10320">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003737">
        <property type="entry name" value="GlcNAc_PI_deacetylase-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024078">
        <property type="entry name" value="LmbE-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993:SF11">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102588">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS63722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="113" mass="13017" checksum="5127448A1AAA0075" modified="2012-04-18" version="1">MINEFVWVYVDLWNIDLIITFNHHGVSGHPNHRAIASALSQSVHTDPKFPTTMMLRSPGIIEKYSSLLGVPYTLYRHHRDRKSLLPQLARPPGHPSRDKKRLAELAHFKRSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTH0</accession>
    <name>H6QTH0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Golgi to ER traffic-protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22094</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178313">
        <property type="protein sequence ID" value="EHS64185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889201.1">
        <property type="nucleotide sequence ID" value="XM_003889152.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTH0"/>
    <dbReference type="GeneID" id="13541183"/>
    <dbReference type="KEGG" id="pgr:PGTG_22094"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22094"/>
    <dbReference type="HOGENOM" id="CLU_698571_0_0_1"/>
    <dbReference type="InParanoid" id="H6QTH0"/>
    <dbReference type="OrthoDB" id="10403103at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053094">
        <property type="entry name" value="Zinc_metalloprotease_ZmpB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193:SF2">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="395" mass="45205" checksum="9611E4A746FC2160" modified="2012-04-18" version="1">MCRSTETGPMNKYNFQKILSSGLIRLQKTIKDSLQHIDPYDNNCGNLNEKYTKQEHETKGLGFAEDWLITKVRFLQLNEDPRKSQATPTQSDGLGFIIKRVWEMLLAISLIYDGRDTCVRMLQLMKSGKQMSEIKNLDGDAGVRKTSFKGNATVEFTPDDWYTFRLKAYSSLTVFLLFGVAGWFHCQNDRRRFNIRDLYSMYALVHQMSLKKATITSKITRSLNNVDSESINPGWERMNQHIVNLLVKSGVGERTLDWFSGLEMWKKQLNPSVLAPLIIKDFFSEVLDPGLTLSSTGSEAPQNIDHQKVLENWQARIPEIFCPPQTAIEKRTLRLHKLGLITVDNSGQDEGCEGRTNEPAEDYTEDIAQLIKDHCTPGSKNHKLYEKMMQVEHTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTH1</accession>
    <name>H6QTH1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22095</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178313">
        <property type="protein sequence ID" value="EHS64186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889202.1">
        <property type="nucleotide sequence ID" value="XM_003889153.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTH1"/>
    <dbReference type="GeneID" id="13541184"/>
    <dbReference type="KEGG" id="pgr:PGTG_22095"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22095"/>
    <dbReference type="HOGENOM" id="CLU_561553_0_0_1"/>
    <dbReference type="InParanoid" id="H6QTH1"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="73"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="157"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="233"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="335"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="377"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="424"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="473"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="486" mass="53101" checksum="F8994CE5F27FE4F5" modified="2012-04-18" version="1">MSKSESSQPTLLSVPALELSQRIAVEVPKSDPPSKTQEPSHLDPLPVKATDLMESEMTPPNHLENVLTEENLAATQTSDTSASSEHVPVTDPTFDPPALLKKSSAANSTSDEPAPIGSNSDTPAPNKNVLVTDPAFDPPAPLKEFSATNPMSEEPASLDSTSDTSASSEDVPVTDPTFDPPSLLKKFSAANSTSDEPAPLENATNPELEPKCLEYQPDQNPILDPLGGIPNAEASNQSNTPQSTAEIRKDDLPPFTYLSGSPQPTEPHPWSQQRHISTSPDRTPFNSPLPYDTPHQSKHATVDSLEPQTVNPLEIFFPEKNHSFPPVDEIPLGDDTSNPSNTSKSLSPRVRQSKGAAENTSQTLDVVVDPLIHSKTQKTSTFRSENMLSNKQLISRKKRRAKDTSEDHTQQASGKRPRNLREESVDDPQDDEEELVDDQQDHEKELVDNQPDDEETTNYGSPVDIKIRHRNQRPQPSKTSTSITLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTP1</accession>
    <name>H6QTP1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22172</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178318">
        <property type="protein sequence ID" value="EHS64256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889135.1">
        <property type="nucleotide sequence ID" value="XM_003889086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTP1"/>
    <dbReference type="GeneID" id="13541355"/>
    <dbReference type="KEGG" id="pgr:PGTG_22172"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22172"/>
    <dbReference type="HOGENOM" id="CLU_2777169_0_0_1"/>
    <dbReference type="InParanoid" id="H6QTP1"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="69" mass="7629" checksum="6050D36984568399" modified="2012-04-18" version="1">MIPTSQPVPNGHKHVYKNLCPPMLYPSTPVPSTTTTSLLASLSQITSPPFTFQSTLLSTRWHFLFLIAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>H6QU36</accession>
    <name>H6QU36_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22262</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178328">
        <property type="protein sequence ID" value="EHS64448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888975.1">
        <property type="nucleotide sequence ID" value="XM_003888926.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QU36"/>
    <dbReference type="GeneID" id="13541054"/>
    <dbReference type="KEGG" id="pgr:PGTG_22262"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22262"/>
    <dbReference type="HOGENOM" id="CLU_3125691_0_0_1"/>
    <dbReference type="InParanoid" id="H6QU36"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="50" mass="5901" checksum="23930315F6F76E98" modified="2012-04-18" version="1">MLPLNQADHLQEVWGVHERDLREVGSTKGAMWMLKYSRRLKCSCRGDELR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQB7</accession>
    <name>E3JQB7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL2m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_00437</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307487.1">
        <property type="nucleotide sequence ID" value="XM_003307439.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQB7"/>
    <dbReference type="FunCoup" id="E3JQB7">
        <property type="interactions" value="95"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JQB7"/>
    <dbReference type="GeneID" id="10528021"/>
    <dbReference type="KEGG" id="pgr:PGTG_00437"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00437"/>
    <dbReference type="eggNOG" id="KOG0438">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036235_3_1_1"/>
    <dbReference type="InParanoid" id="E3JQB7"/>
    <dbReference type="OMA" id="TAQRGNC"/>
    <dbReference type="OrthoDB" id="268576at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000128">
        <property type="entry name" value="50S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.950.10:FF:000001">
        <property type="entry name" value="50S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="entry name" value="Ribosomal protein L2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_uL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_uL2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022671">
        <property type="entry name" value="Ribosomal_uL2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_uL2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Ribosomal_uL2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL2M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="RIBOSOMAL PROTEIN L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00181">
        <property type="entry name" value="Ribosomal_L2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00467">
        <property type="entry name" value="RIBOSOMAL_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL2 RNA-binding" evidence="10">
        <location>
            <begin position="89"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL2 C-terminal" evidence="9">
        <location>
            <begin position="227"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="270"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="377"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="280"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="423"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00037226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00069872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP74481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="432" mass="47537" checksum="9D41EE414BF63909" modified="2011-01-11" version="1">MAFLRTILGRTTTTSKLLNPLNQQQANQFSIGQQLFVWGQRKKNQVDKNFKIYKPVTPAIRHLRLPRNDHLHKGRCYLPLTLPKKKTGGRNNSGRITTRHIGGGHKQRLRMVDFHRNLSGPQLVVRIEYDPGRSAHIALIKHVQSGALSYILAPLNLRPGQIVQSFKTGIPKDLLNSSNSILDQPTPEANPAGQPSTVASQGVGELISAKEVSNSTLTLGLLRTLTIKPGNYLPIRLIPAGTTIHAISLKPDGKASLVRSAGTFAKVVSLGHHHRKTKASSKQASSVQQQQQQQQQQQSTGAQEQKTDQRDLHDLQDHQGDYAQIKLQSGEIRFVHMDACAAIGSVSNPHWQGRKLGKAGRVRWLGRRPRVRGVAMNKVDHPLGGGRGKSKSNKQPVSPAGLKAKGLRTRRPGPRGNQMVVKQRPKGKHVGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRQ2</accession>
    <name>E3JRQ2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_00501</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307551.1">
        <property type="nucleotide sequence ID" value="XM_003307503.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JRQ2"/>
    <dbReference type="GeneID" id="10546449"/>
    <dbReference type="KEGG" id="pgr:PGTG_00501"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00501"/>
    <dbReference type="HOGENOM" id="CLU_1468866_0_0_1"/>
    <dbReference type="InParanoid" id="E3JRQ2"/>
    <dbReference type="OrthoDB" id="10311410at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171481" evidence="2">
        <location>
            <begin position="17"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP74545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="221" mass="23532" checksum="7A479DAB19C30724" modified="2011-01-11" version="1">MHSRFMLAGLCSISTTFDGVIGPAIEGGAERGGIGLSHTPPVALARQDPAGDGEQLTSGVAQFDFPETSTTAALADEDQKRRFLFGKPIPLETPIKTNKMKTTGDGKERPSNSAWVDVPETFTSAVAVPDGRQGQELPDNPIQLYTPVEANKLTTINDGENQSSNVAPIDISRTSTSVGRSDGYQERELLFEDLIHLDTPVAGNSGGPLRYTTLRAHVALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRS4</accession>
    <name>E3JRS4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_00164</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307214.1">
        <property type="nucleotide sequence ID" value="XM_003307166.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JRS4"/>
    <dbReference type="GeneID" id="10528195"/>
    <dbReference type="KEGG" id="pgr:PGTG_00164"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00164"/>
    <dbReference type="HOGENOM" id="CLU_1960629_0_0_1"/>
    <dbReference type="InParanoid" id="E3JRS4"/>
    <dbReference type="OMA" id="RDLHTET"/>
    <dbReference type="OrthoDB" id="2509735at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171484" evidence="2">
        <location>
            <begin position="17"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP74208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="118" mass="13273" checksum="0C220447E498400F" modified="2011-01-11" version="1">MLHLIRTLFSLNLVLAFTVAAYPSQSALLQNVPHGLGKRFQTVHAEASAPRPWVKTARDFIQEAPQDMTAERAEKRSSQPIPRSFSRIRRRPEAGIATSRQTTGISNPRPWVQKDEAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3JSM7</accession>
    <name>E3JSM7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01645</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319471.1">
        <property type="nucleotide sequence ID" value="XM_003319423.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JSM7"/>
    <dbReference type="STRING" id="418459.E3JSM7"/>
    <dbReference type="GeneID" id="10547414"/>
    <dbReference type="KEGG" id="pgr:PGTG_01645"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01645"/>
    <dbReference type="HOGENOM" id="CLU_1180696_0_0_1"/>
    <dbReference type="InParanoid" id="E3JSM7"/>
    <dbReference type="OMA" id="CHNPSII"/>
    <dbReference type="OrthoDB" id="2499382at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="235" mass="26567" checksum="3FF82E415363D497" modified="2011-01-11" version="1">MTMFMLSWCQNPSIIMMHKVRLFCHIGLAIMAVLMLSSTSVILAYSIKMYHGTFLHSILEILIASAVSFIIVGLAWLPSNEHLGSYGLSSMRAVWQELLWSSILTTGIAASLASMHESTPGLMTSCGRYFICRGYIFIFIVTWLGWAMIALPTILLLVSALYQMKRSPIGGSVWQVDIDRFEYFWPGQTHLKSRRVTMINELPRVQPDLADLKSHHDGFPFENEKRATGASFHNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3JT61</accession>
    <name>E3JT61_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="10">NADPH oxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_01933</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75340.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319759.2">
        <property type="nucleotide sequence ID" value="XM_003319711.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JT61"/>
    <dbReference type="STRING" id="418459.E3JT61"/>
    <dbReference type="GeneID" id="10542806"/>
    <dbReference type="KEGG" id="pgr:PGTG_01933"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01933"/>
    <dbReference type="eggNOG" id="KOG0039">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005646_3_0_1"/>
    <dbReference type="InParanoid" id="E3JT61"/>
    <dbReference type="OrthoDB" id="167398at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043020">
        <property type="term" value="C:NADPH oxidase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016175">
        <property type="term" value="F:superoxide-generating NAD(P)H oxidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006952">
        <property type="term" value="P:defense response"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006811">
        <property type="term" value="P:monoatomic ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042554">
        <property type="term" value="P:superoxide anion generation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06186">
        <property type="entry name" value="NOX_Duox_like_FAD_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000091">
        <property type="entry name" value="NADPH oxidase (NoxA), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000778">
        <property type="entry name" value="Cyt_b245_heavy_chain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013112">
        <property type="entry name" value="FAD-bd_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013130">
        <property type="entry name" value="Fe3_Rdtase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013121">
        <property type="entry name" value="Fe_red_NAD-bd_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050369">
        <property type="entry name" value="RBOH/FRE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972:SF39">
        <property type="entry name" value="FAD-BINDING FR-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11972">
        <property type="entry name" value="NADPH OXIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08022">
        <property type="entry name" value="FAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01794">
        <property type="entry name" value="Ferric_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08030">
        <property type="entry name" value="NAD_binding_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00466">
        <property type="entry name" value="GP91PHOX"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00052">
        <property type="entry name" value="Ferric_Reductase_Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01168">
        <property type="entry name" value="Ferric_reductase_subgroup_(FRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01169">
        <property type="entry name" value="NADPH_oxidase_subgroup_(NOX)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="45"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="197"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding FR-type" evidence="9">
        <location>
            <begin position="217"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP75340.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="532" mass="61259" checksum="8A7C9857A5D483BC" modified="2012-04-18" version="2">MLSRRTTFYIIWGAIHLFIFIYGWWKQETDQRLAALNKLEYSVWISRGAGLCLCLDGFALFIPVCRNLMHLLRPRIGWLVSVDSNIWFHRQVAYTTLFFTAIHTTAHYVNMFHVETTQIRPEKAIEIMYSETGPLTGHIMLFIMVLMYTTASTKIRTQCFEAFWYTHHLAFFWALCLYTHAAGCFVRGALPDHKAQCLGYNSVYVTVWSGLAYFCDRVFREIRGRGRSEISAVLIHPSGTVEIRMLKAGFKYVPGQWIFFQMPEVSRFQWHPFTISSAPDDPYISIHVRQVGDFTKAVGTRLGATPQLMATLNQPSEFSVEDCGEFHDITTIRSRDLPLVRIDGPYGSPAQDVFKCEVAILIGAGIGVTPFSSILKNIYYMQAQGKLGLLRKVQFIWINKEITSFSWFKTLLKNLEDIQHDYSFLRMDMYLTGSMDEDTISNVVLNTGAHKFDSLTGLKSQTHFGKPHWKKDVFDPIRKAIHSGDWYERDISGTTKVGCFYCGPRPLAKTLEQECRQATTDKVKFEFHKERF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3JTF5</accession>
    <name>E3JTF5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_01822</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75229.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319648.2">
        <property type="nucleotide sequence ID" value="XM_003319600.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JTF5"/>
    <dbReference type="GeneID" id="10542603"/>
    <dbReference type="KEGG" id="pgr:PGTG_01822"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01822"/>
    <dbReference type="HOGENOM" id="CLU_1107568_0_0_1"/>
    <dbReference type="InParanoid" id="E3JTF5"/>
    <dbReference type="OrthoDB" id="10313854at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173201" evidence="2">
        <location>
            <begin position="28"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP75229.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="251" mass="28150" checksum="3981FE43FE851634" modified="2012-04-18" version="2">MLLTHRFSLPLPHLVLLVCLFKLSGTSLPIGIHAGSKVQTISPSEHSSSECNLNLIKDINASEIQPNGKRRKTTPSDSSDKTGLTDFYNFDPEGLEGEWWETIMTEFPAINGLEHGAVTPITIDNADPSEFEANNVQYAPPDKMDAQRQTQRMKEPQDITNQQGVSGTHPEMLSHGNRRHELPKPKPTYNVGNEELEIIRFSSTQKSKSLYGLANKALTTYGAKSETLYKIKMNFERKIFKNITALRLIQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUL1</accession>
    <name>E3JUL1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Vacuolar protein sorting-associated protein 54 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_01067</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
            <location evidence="1">trans-Golgi network</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the VPS54 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75736.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320155.2">
        <property type="nucleotide sequence ID" value="XM_003320107.2"/>
    </dbReference>
    <dbReference type="FunCoup" id="E3JUL1">
        <property type="interactions" value="256"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JUL1"/>
    <dbReference type="GeneID" id="10543616"/>
    <dbReference type="KEGG" id="pgr:PGTG_01067"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01067"/>
    <dbReference type="eggNOG" id="KOG2115">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003094_2_0_1"/>
    <dbReference type="InParanoid" id="E3JUL1"/>
    <dbReference type="OrthoDB" id="10259024at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000938">
        <property type="term" value="C:GARP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042147">
        <property type="term" value="P:retrograde transport, endosome to Golgi"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.860">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039745">
        <property type="entry name" value="Vps54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012501">
        <property type="entry name" value="Vps54_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12965">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12965:SF0">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07928">
        <property type="entry name" value="Vps54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Vacuolar protein sorting-associated protein 54 C-terminal" evidence="9">
        <location>
            <begin position="817"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="157"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="211"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="458"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="619"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1157"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1311"/>
            <end position="1644"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="293"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="17"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="157"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="172"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="211"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="234"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="466"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1169"/>
            <end position="1185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1226"/>
            <end position="1258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1259"/>
            <end position="1275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1338"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1399"/>
            <end position="1417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1418"/>
            <end position="1434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1435"/>
            <end position="1459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1484"/>
            <end position="1526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="1542"/>
            <end position="1557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1573"/>
            <end position="1594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1600"/>
            <end position="1618"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="8">
        <location>
            <begin position="1634"/>
            <end position="1644"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP75736.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1644" mass="178611" checksum="D5452863A3314388" modified="2012-04-18" version="2">MSTRPSIIRNNQNEIGNEMEDENENENENENGNDEGKPFIFDQNTHYHPNYPISTVMNQVSSQNITVAAFNAIRSSIPINLKPPPLSSFVKLPYGKQHSVLAPSNKRSAPSPIKLSRSPTIPLSDFDSYLEQVQVHYDRWQQNLATNSKAIKSLHPNHSTDLLVNPTNQSDHSSAQALHPSPSLPSLSSVPHVFFDQKFSLNDPLTFDLLTQQPSQSSPITSPTKSDLLDSTHPKPSTSSTSSNTDLTTDHILLDKLSHYLDLVELHLVQEISLRSSDFFSALGNLQALHAQTSSSVAQIEALKNQLDQLKQTVSDKALKIIRYGIRRRNITSIELALTKLNDVWQTVHGIEELIQSGEWQSALGLIQDLEQLWHSSTVLSSASSHQSTDSNQSSVPPPAPPIKLRLSKLKALAALPKRLSVFRSTISKALVAELSSILSHDLKINLAEFASSHSLDQPQSFDLKSPMSSPLSSSHPNGSQTRDRLKSLTQVRLKERVRSTFDGLVRAGGMDKALGAWKESVIKQIREIVKNALSDVIEGDLDDKDDSSDISQFNSKDKRNALSDKTISLARNLKALKHEDFLKIAEKTYRDLLGCIELVDTQSQALLELTEEFKTQTSPVTSDLPVDPPADSNPAINPSGLEPIATDADQSSTVSAIDVEAFSLKLNEVVQSSAELANARFAKVIGVRTEVHAQLSFTDFFAIFDASWRFVVQCEVISRRMIIALRGVMVNQAKAFLQSFHQNKITESAKIVEQEQWTPVEVPNQVQQIVNSIIRSAMEDPKSLVINRNLSNLPAEDEIAEPNSSTKLLEIEGTFYHPVSASLQTMKTLADYLQVVVNCSLLTTDLMGKIIEFLKAFNSRTCQVVLGAGAIRSAGLKNITAKHLALASQALSTMISLIPYIRECLRRHLNMKQAVMLVDFDKLKRDYQEHQNEVHAKLISIMADRLGVHKQTLEAMDWENIETTSSDQVLGPNAYLESLLKEVGTLHRVLGRYLSASTLQHILSQVFMSIHGALHEEFSKVELKSTQAKERLLRDAQYFSTKLVELKASEDCQASGKSLEELVQTRFFPKAGVPSINGNDLKGPDAAQYPLPPSPLAERSSNFRAKFRFLSPRGRAVDLSASLLAEVTNAATSPTSGEATRIVSPVGSVLEAEIDIDGSKPSDGSAIAHRDTSQERSEAVKDGSGEPDPSAQELPALQTNPSSAPRTPEPKDEVPAVAEPSSHEASVPSPSTSAASRPLKLSLSQRLAALASSRRGSQPSIVTSPLQSGLSTTPEPLASEDTHKNNETADSSASLLAGMTNAAISPTIGEAARTVSPGGSVVEAETNIDGSKPFDSSPITHQDTTQETSEGAKEASEQPVPSDPSAQELPVLETNPSSAPRTPEAKDEAPAGAEPSPHDPSVPVPPTAVAPRPPKPSLSQRLAALASSRRGSQPSIVTSAPQTDLSSTPEALTSEPTSKNIETIEKNNETIEEDSEAIGKNNETIDKNDETIEKNDETIDKNNETIDKNDETIKLLKEKEKEARNAEANPGLLDPVNSKGEDEDEGDDGIETEEVNLIDNSLISVHEESKIEANSSSDFPTDSHENPSATSDPSGLIPTAPPPPPPPPAPLLPPAPRIPLNQRLAALASSRRNPPPPPSESLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUM5</accession>
    <name>E3JUM5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_01081</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320169.1">
        <property type="nucleotide sequence ID" value="XM_003320121.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JUM5"/>
    <dbReference type="GeneID" id="10543742"/>
    <dbReference type="KEGG" id="pgr:PGTG_01081"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01081"/>
    <dbReference type="HOGENOM" id="CLU_045572_1_0_1"/>
    <dbReference type="InParanoid" id="E3JUM5"/>
    <dbReference type="OrthoDB" id="10278074at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP75750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="291" mass="32950" checksum="005A8EED9B200412" modified="2011-01-11" version="1">MSDCRIPLLSLGIKTKLAQPDLETSIAQGSNNSSPTPTSAVPFAFTDHVKRFMQDHLRHILAKGNVDAYTRTMSIVDSAPIRRTPLLLLKAHVQSQDPLFHRDYLPPGYPTDIGASCKVVELMKRLLKSEKGLLRNLLLTNIRAQKDRLISGAVPCLDDLVVCIDRYMAARKQLRAVEVILRSYPSTTRTRLAFLRLYIVDHLIHRDPADNTSHWELVDQQLEYVRGQSKLYRIAYGRVVKAIDWELFGQKRKFEEIPHNDIRVPTEDDVQEQIALMSAGGGSVPLENAFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWS3</accession>
    <name>E3JWS3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_02939</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76498.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320917.2">
        <property type="nucleotide sequence ID" value="XM_003320869.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JWS3"/>
    <dbReference type="STRING" id="418459.E3JWS3"/>
    <dbReference type="GeneID" id="10528271"/>
    <dbReference type="KEGG" id="pgr:PGTG_02939"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02939"/>
    <dbReference type="eggNOG" id="ENOG502T2J0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073655_0_0_1"/>
    <dbReference type="InParanoid" id="E3JWS3"/>
    <dbReference type="OrthoDB" id="160054at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35396">
        <property type="entry name" value="SMALL SECRETED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35396:SF1">
        <property type="entry name" value="SMALL SECRETED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172199" evidence="2">
        <location>
            <begin position="22"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="186"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="279"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="305"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="316"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP76498.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="327" mass="35657" checksum="D2EFF56CB9DBA4DF" modified="2012-04-18" version="2">MGFSCLTAFFISFFLAGLVSANWDPSTGYLHNYRPSPAWLNKFKPSESSPHVQVSECSINTRLHYPKVQVFAWFEVNHVWDGNHGCPYGTCHAYVVHPAAADMVPSFTEGHSFFWHKTGGLPGNGVKPISNPRHGGFGYEVSLTGKFIDGPANTQSQQLDHDGKYPGFNKKKLQLWSQAEMNAFKGTDQSQKHPKCGTPCGPNKDPGSAPGAYGGYKPASASTYKPPKGWKPNKGDTCFKNGPDLLPKPDPSKPGKGSGNLNKPKDEDSTTDPKHKNADNSTTKPKNQQQDSSTTDPSNKKDGSSTKTTKSSSNPTHRKKSLRKKCT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZ79</accession>
    <name>E3JZ79_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_03310</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the Nudix hydrolase family. DCP2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321773.1">
        <property type="nucleotide sequence ID" value="XM_003321725.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZ79"/>
    <dbReference type="SMR" id="E3JZ79"/>
    <dbReference type="STRING" id="418459.E3JZ79"/>
    <dbReference type="GeneID" id="10538062"/>
    <dbReference type="KEGG" id="pgr:PGTG_03310"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03310"/>
    <dbReference type="eggNOG" id="KOG2937">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_491869_0_0_1"/>
    <dbReference type="InParanoid" id="E3JZ79"/>
    <dbReference type="OMA" id="HTRFENE"/>
    <dbReference type="OrthoDB" id="18996at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000932">
        <property type="term" value="C:P-body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140933">
        <property type="term" value="F:5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000290">
        <property type="term" value="P:deadenylation-dependent decapping of nuclear-transcribed mRNA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000184">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03672">
        <property type="entry name" value="NUDIX_Dcp2p_Nudt20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1050">
        <property type="entry name" value="Dcp2, box A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007722">
        <property type="entry name" value="DCP2_BoxA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036189">
        <property type="entry name" value="DCP2_BoxA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044099">
        <property type="entry name" value="Dcp2_NUDIX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23114">
        <property type="entry name" value="M7GPPPN-MRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23114:SF17">
        <property type="entry name" value="M7GPPPN-MRNA HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05026">
        <property type="entry name" value="DCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01125">
        <property type="entry name" value="DCP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140586">
        <property type="entry name" value="Dcp2 domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0464">Manganese</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="10">
        <location>
            <begin position="136"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="215"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="365"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="412"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="222"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="365"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="413"/>
            <end position="430"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="446"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="456"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP77354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="554" mass="62649" checksum="5ACB5F469C65388A" modified="2011-01-11" version="1">MPSSPSSSNNNHQHQHQQPRQSSSATLPATSIASSASVFSRMPLEDVLEDLASRFILNLPIVELSHIERVCFQVEQAHWFYEDFVRPNSLLNLPSYHLKTFTGLFFEKCDFLTVDGAPLAGWDPKTAYEKFMRYKERVPVCGAIMFNEDATQVLLVRGFKSNSSWSFPRGKINENELPKDCAIREVLEETGFNIEPYLSLPSSWSDDSKLSAIRKIRPTNPTPNGSNSQSHKPHQNCTLHSEGDDHFIEITIREQRLRMYLVTGIPNDTKFVTQTRQEIGRIAWIPLSDLPTFSASNGTLGSKQLSKMINVSCPSTVESQRAHAKFYMVVPFVNDMRTWLTNNHLLAPIGQSLPTPREHHQAYVQTNHPPTNQSYGGNPIGHPPSHHIRFENEAAAVHERVSRLQAFDFFQEEPSSTEQVNQDNRLNPSTNRKKNRRKQPQQPRKPPKEANHQRLTDNPSYHSDPQTSTDDEDITKVNNDTPWNDLAEGTRALQNFFFGSRNEELEGGGDRFGLLSDGPTALLDLHLRHDAANNGVSPLIASQLHATPDRLVHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3K0B7</accession>
    <name>E3K0B7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_03698</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322161.1">
        <property type="nucleotide sequence ID" value="XM_003322113.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K0B7"/>
    <dbReference type="STRING" id="418459.E3K0B7"/>
    <dbReference type="GeneID" id="10540564"/>
    <dbReference type="KEGG" id="pgr:PGTG_03698"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03698"/>
    <dbReference type="eggNOG" id="ENOG502QPKG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044506_0_0_1"/>
    <dbReference type="InParanoid" id="E3K0B7"/>
    <dbReference type="OMA" id="YECMANA"/>
    <dbReference type="OrthoDB" id="2496699at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024752">
        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46929">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46929:SF3">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12776">
        <property type="entry name" value="Myb_DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like" evidence="2">
        <location>
            <begin position="105"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="243"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP77742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="352" mass="38583" checksum="7739A60347E7F681" modified="2011-01-11" version="1">MPRGRKEKNSQPILATQVSQQSSAAHTKLLSLGSGTTGTPARTTHGLQPSLATPSVIDQPENTPPNSKTGISSQNQPPNSETRPEGTNSQNQPEGDNSHRKNLVWTPAMEKSALDLYVKAVEEGKRCNNGFKPETHRWVATELSNEFPGSEFTGPKVKSKLNQTFKKWYDAFVACKEASGFGWNEDQCMVTASEEVWSSFLVSHPTAKRFKNTPFPEFHELNVIFSGNAAVGALCKGTNAIEAESNQESPPDAINNGQNLPSTASQEVRSARPGVRPPRKHRISSGDRFENTVERLIDAFISQDDNTAQTSTIHRAIEKFQDHFAEGLDMDDMVAGFSILENEAKANTFLAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3K191</accession>
    <name>E3K191_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GCM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP78066.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322485.2">
        <property type="nucleotide sequence ID" value="XM_003322437.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K191"/>
    <dbReference type="GeneID" id="10541536"/>
    <dbReference type="KEGG" id="pgr:PGTG_04022"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04022"/>
    <dbReference type="HOGENOM" id="CLU_016062_2_0_1"/>
    <dbReference type="InParanoid" id="E3K191"/>
    <dbReference type="OrthoDB" id="3046222at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78066.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="652" mass="73497" checksum="C232A3D7B6DE651B" modified="2012-04-18" version="2">MAQVRLKSRGTTLPDETPAGNDPNDDTAKSDDSEYFDPHNSKGDDPNDADDPNDADNPNIASGEDELSAAKKKSNPKKWFLPDMNEKYTTYIDHGCILDKQGYPLYPNGNTTFLRLPEDDITNFGMVGYSRTSSSNRRGRNNCWKVTRYFCLGAMTCDNPDCNWAGAPSTNKQKLEELNQKKMPMKCPGFSGNCLGNVSHSKCNLAQVRFDEHASGWGLLRHSGDHSHPWPEAKKPDPLSQLELAREIQKNPQAGAFKLKLRKGVDPNAPSQSVVTIHPAYGHKDRTAYHRRKMLVASGLKPDELGGGIGDKFILDMFAWAAQGLLVILSSFLPNSEHFTFQTPWMADRLLARDKNNALYSGGFLSDVTYRSFANGYLLTTSMYCEELGRWIPVQFTWLRGLSEEYYKIHFTTFFRQFFNRSMTHLERDALACQVVDFSAAQAKGFVSAYIEEPFRQACLALMDLEEPDGPTHEEKVDELYRRYPKVKKWLDWWTHADVEGMIFCRRRDKLPDTTNAQESMHRLYYKITEGKVCLYVGMVELFSFVKTLEEDWTAVMKGVAISYGSQKVKDVGVSMGFPAKRKRKHESANDGRAPDTVAQPMLLYTTATKSCFGPASPMFIGSWGLSPTFNIDLGFLGVVVSPVKRNKPPTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3K1M3</accession>
    <name>E3K1M3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_04154</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. COT1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP78198.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322617.2">
        <property type="nucleotide sequence ID" value="XM_003322569.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K1M3"/>
    <dbReference type="STRING" id="418459.E3K1M3"/>
    <dbReference type="GeneID" id="10541828"/>
    <dbReference type="KEGG" id="pgr:PGTG_04154"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04154"/>
    <dbReference type="HOGENOM" id="CLU_000288_67_2_1"/>
    <dbReference type="InParanoid" id="E3K1M3"/>
    <dbReference type="OrthoDB" id="3638488at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007163">
        <property type="term" value="P:establishment or maintenance of cell polarity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000024">
        <property type="entry name" value="Probable serine/threonine-protein kinase cot-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017892">
        <property type="entry name" value="Pkinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050839">
        <property type="entry name" value="Rho-assoc_Ser/Thr_Kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988:SF76">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_135, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22988">
        <property type="entry name" value="MYOTONIC DYSTROPHY S/T KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00433">
        <property type="entry name" value="Pkinase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00133">
        <property type="entry name" value="S_TK_X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="11">
        <location>
            <begin position="1"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="12">
        <location>
            <begin position="271"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP78198.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="388" mass="43942" checksum="3884A0A8989BF8F9" modified="2012-04-18" version="2">MLRCPCRPYEPEKHYVYFSGNPSQFSKPILEKTTACKLKIEHVSRKAVEINESSFLNQIRIDRIQKGQSDPGRAPLYDTISEDVTRFYMSECVLALEAVHKLGFIHRDIKPGKILIDKDGHVKLSDFGLSTGFHKQHDSARDFLDQSDGVLEKDQIATCKANRRKLAFSTVGTPDYIAPEIFLQQGYNKECDWWSLGAIMFECLVGYPPFCSPSAHETYRKIIDWRHELYFPDDVHLSRESEDLIRRLITSADHRLGKKGAEEIKDHVFFSGVDWTTIRNIEAPCVPHLKSVTDTSYFPTEDSDDLPTEPVGADTDTSSRDLAFLGYTFRRYENSGAVSQAMVRVLQINLHLESCSLPASISIHTTHQTQISALNASPNIYPAGDAQH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3K1Y3</accession>
    <name>E3K1Y3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Calcineurin subunit B</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Calcineurin regulatory subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Protein phosphatase 2B regulatory subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_04308</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity. Plays a central role in virulence and antifungal drug action.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Composed of a catalytic subunit (A) and a regulatory subunit (B).</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the calcineurin regulatory subunit family.</text>
    </comment>
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        <property type="protein sequence ID" value="EFP78352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003322723.2"/>
    </dbReference>
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    <dbReference type="FunCoup" id="E3K1Y3">
        <property type="interactions" value="219"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K1Y3"/>
    <dbReference type="GeneID" id="10542306"/>
    <dbReference type="KEGG" id="pgr:PGTG_04308"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04308"/>
    <dbReference type="eggNOG" id="KOG0034">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061288_10_1_1"/>
    <dbReference type="InParanoid" id="E3K1Y3"/>
    <dbReference type="OMA" id="DTNFDRD"/>
    <dbReference type="OrthoDB" id="191686at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005955">
        <property type="term" value="C:calcineurin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004723">
        <property type="term" value="F:calcium-dependent protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008597">
        <property type="term" value="F:calcium-dependent protein serine/threonine phosphatase regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019902">
        <property type="term" value="F:phosphatase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097720">
        <property type="term" value="P:calcineurin-mediated signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071444">
        <property type="term" value="P:cellular response to pheromone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000747">
        <property type="term" value="P:conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006873">
        <property type="term" value="P:intracellular monoatomic ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
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        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000047">
        <property type="entry name" value="Calcineurin subunit B type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45942">
        <property type="entry name" value="PROTEIN PHOSPATASE 3 REGULATORY SUBUNIT B ALPHA ISOFORM TYPE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13499">
        <property type="entry name" value="EF-hand_7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="21"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="60"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="90"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="10">
        <location>
            <begin position="131"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00053864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP78352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="175" mass="19757" checksum="E39338E72A2476C4" modified="2011-01-11" version="1">MGQTGSQLLQEIEKSSNFTGDEIQRLKKRFMKLDRDQSGSIDKDEFLQIPAIANNPLASRMIAIFDEDGGGTVDFQEFVDGLSAFSSRGDREQKLKFAFKVYDMDRDGFISNGELFLVLKMMVGNNLKDQQLQQIVDKTIMEADTDCDGKLSFDEFKQVVANTDIAKQMTLEALF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3K1Y5</accession>
    <name>E3K1Y5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322773.1">
        <property type="nucleotide sequence ID" value="XM_003322725.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K1Y5"/>
    <dbReference type="GeneID" id="10542308"/>
    <dbReference type="KEGG" id="pgr:PGTG_04310"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04310"/>
    <dbReference type="HOGENOM" id="CLU_462419_0_0_1"/>
    <dbReference type="InParanoid" id="E3K1Y5"/>
    <dbReference type="OMA" id="QHQYESI"/>
    <dbReference type="OrthoDB" id="2507189at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="214"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="522"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="574"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="214"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="243"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="309"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="347"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="522"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="541"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="650" mass="70586" checksum="97B93BE0A3614B4E" modified="2011-01-11" version="1">MEHQQQQQQQQQQHHHQQQQYQQHPYQQQLQHQYQQQQQYQQPPQQYQQQQQQHYLQQPQHYQQQQQQPRSEVILTDQIDQLDQQLQATFRSIANIINYNLQLRKLNITPFIQSNQNHLPPKELLEQLKQNLNGFDGTLLQIEKIATVAIAITKRDLDHAIQSQPPPAPPPTTTVTDQNQMEIELISNQHQQTPSTQQQPIIIEQEPEVEILNNPKTHPQTSLTQPIDLTSSQSPSPQPPNPIEQTTTPTQDPRPSEQPSGADKLIPPPDQPEIDNQTKPEAQNQGPIEIDLERDSSPETALNNVSPVKATSKEGSSSSSSAACSPTSANPSNERPGTATMDLKSASDLTDGDLFGDGSLFGSSHHGSVVPSPLNQHAHVTIPPTATEKDPNNELQNVQTTVPSQGSNETQTDRPPAIPIMASQQQQSSTTDPSAQLSVPAQDQNLLSTTTSLTNTGSLDSSSSMDMITTQADLSAFLSSFSHSPSLPHSGGPSQTASPAKRTLEQVPGLIGLGIDLAFPSSSSSNTHNNNNRLSTEPVSNNPLFSSLTNNNNNNTHLNINPLVSNAHHLALPTTSFNNTTTPSSSSTSPAAAAASSASSADPAALPTPSPLAHLPTPSSNNNNNGAVTNLNPVDFEAMFANIQSHLPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3K235</accession>
    <name>E3K235_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UmuC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_04360</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78404.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322823.2">
        <property type="nucleotide sequence ID" value="XM_003322775.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K235"/>
    <dbReference type="STRING" id="418459.E3K235"/>
    <dbReference type="GeneID" id="10536002"/>
    <dbReference type="KEGG" id="pgr:PGTG_04360"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04360"/>
    <dbReference type="eggNOG" id="KOG2095">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_064553_0_0_1"/>
    <dbReference type="InParanoid" id="E3K235"/>
    <dbReference type="OrthoDB" id="5723at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005657">
        <property type="term" value="C:replication fork"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009314">
        <property type="term" value="P:response to radiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000014">
        <property type="entry name" value="Polymerase (DNA directed), eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052230">
        <property type="entry name" value="DNA_polymerase_eta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001126">
        <property type="entry name" value="UmuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45873">
        <property type="entry name" value="DNA POLYMERASE ETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45873:SF1">
        <property type="entry name" value="DNA POLYMERASE ETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00817">
        <property type="entry name" value="IMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21704">
        <property type="entry name" value="POLH-Rev1_HhH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50173">
        <property type="entry name" value="UMUC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="5" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="UmuC" evidence="7">
        <location>
            <begin position="37"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP78404.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="366" mass="41223" checksum="5A6406694C390355" modified="2012-04-18" version="2">MAHPQRPRETLSAAESVHSHRLGETLLADESVHSHWPRETLLAAKVSLDPYRRESVKILKIFSEICPTIEKASIDEAFLDFSIPDSSSEISLDDPLPNPPPLDLEDLLRSSQSNLVPLDADSDYPTNTCTDIALLIGTELMARCRQQVFDRLGYTCSAGIATNKMLAKLCSAYKKPNAQTVLRAGAVRDFLRPFELSKLRFLGGKLGQSVSELVDSTCDKLCQLSKVWKVPLSQLQASLGEQTGMWVWESVHDVNRSEVETKTLVKSMMSSKNLRPSISSRPMKVYTLISRERFPWPMRVYTLISQECSSRCFSRPMRVYTLISQECFSRPMRVYTLSGQECFSQPLRVCHQRPGGILPAVAGYFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2Q7</accession>
    <name>E3K2Q7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">arginine--tRNA ligase</fullName>
            <ecNumber evidence="2">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Arginyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_04582</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>tRNA(Arg) + L-arginine + ATP = L-arginyl-tRNA(Arg) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="2"/>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323045.1">
        <property type="nucleotide sequence ID" value="XM_003322997.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2Q7"/>
    <dbReference type="FunCoup" id="E3K2Q7">
        <property type="interactions" value="608"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K2Q7"/>
    <dbReference type="GeneID" id="10536479"/>
    <dbReference type="KEGG" id="pgr:PGTG_04582"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04582"/>
    <dbReference type="eggNOG" id="KOG1195">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006406_6_2_1"/>
    <dbReference type="InParanoid" id="E3K2Q7"/>
    <dbReference type="OMA" id="YEFKWER"/>
    <dbReference type="OrthoDB" id="68056at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07956">
        <property type="entry name" value="Anticodon_Ia_Arg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00671">
        <property type="entry name" value="ArgRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000006">
        <property type="entry name" value="Arginyl-tRNA synthetase 2, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000058">
        <property type="entry name" value="Mitochondrial arginyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.70">
        <property type="entry name" value="Arginyl tRNA synthetase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036695">
        <property type="entry name" value="Arg-tRNA-synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00456">
        <property type="entry name" value="argS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956:SF11">
        <property type="entry name" value="ARGININE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="ARGINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55190">
        <property type="entry name" value="Arginyl-tRNA synthetase (ArgRS), N-terminal 'additional' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DALR anticodon binding" evidence="12">
        <location>
            <begin position="502"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP78626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="626" mass="70323" checksum="50A9EFC942AE44D4" modified="2011-01-11" version="1">MDPPSGPKSTSDVPLLASLPPLPIPTGAEPSRNVLDAFRTAVAVQVAEILNLPHSDPADLERIWAGVESGKKEGGDLTIAVPRFRIKGSDPKQLAKKIQDEFQTNEWIQSANAEGAFVKFQMDSKTLCRTVLHQVNELTHLSNTGGGYGTNSSGIDPSTGKKKKLVLEFSSPNIAKKFHAGHLRSTIIGAFLGNLHEANGWDVKRINYLGDWGRQFGVLAVGFERYGSEQELSEQPIQHLLDVYTKINKDMEGEEKEALEQKARDFFKLMEEGDSTAVALWKRFRDYSIKQYEQDYARLNIKFDVYSGESQVTHESMDVALKVLREKGIAIEDKGTLICDLDAYKLGRTVIRKSDGTNLYITRDIGEAIQRYDKYQFDKMIYVVASQQDLHLAQFFKVLDLMGFPWAKKLQHINFGMVAGMSTRKGTSVHLEDMMNEARNTMHEQMKTNVDKYNEIPDPLRTADEVGISGIKIQDMAGKRINGYEFKWDRVLSFEGDTGPYLQYAHVRLCSVERKAAPTYVLPEPTKIAAEIETSILSEEAKARELVVLLSLYPDAVKTAFSKLEPSSIVTYCWRLTHAVSSAWETLIVKGQDQPTALARLWLFRSCKDVLASAMKLLTLEPLDRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3K382</accession>
    <name>E3K382_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_04895</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78939.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323358.2">
        <property type="nucleotide sequence ID" value="XM_003323310.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K382"/>
    <dbReference type="STRING" id="418459.E3K382"/>
    <dbReference type="GeneID" id="10537127"/>
    <dbReference type="KEGG" id="pgr:PGTG_04895"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04895"/>
    <dbReference type="eggNOG" id="KOG0244">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007266_0_0_1"/>
    <dbReference type="InParanoid" id="E3K382"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23159">
        <property type="entry name" value="CENTROSOMAL PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23159:SF31">
        <property type="entry name" value="CENTROSOME-ASSOCIATED PROTEIN CEP250 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="236"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="363"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="475"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="604"/>
            <end position="642"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="569"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="862"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="949"/>
            <end position="1001"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1107"/>
            <end position="1215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="246"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="373"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="631"/>
            <end position="642"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP78939.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1234" mass="138859" checksum="5CD368BDEAD9CE43" modified="2012-04-18" version="2">MNSDSFLTSDDGPYRNAPFHLGAQVTSYSDCPVDSDAGVGSSIAPSSPHLNFKSIGTTQAILHYDDPARSAPQIASFDGPQVQAQTAYLETTVEKLRTELARVRDSNSITDLSNSRLSLSSIERDLASKFDSPSSKYRHVSRHFAQLQGVYGKAFSDIFKVVSAEAGPRSNLNSIQSTLESSIDPIAEEYKSFIGSLESQLDTLAGTPTKRNTRTEAAPTSTVLTSSASRFDPFATQKEESDVKTTRLQTPSSEAAANDICMTERSPTFHQDGRSAVAEVHEGEGKRLPGLKTEMEQPEIVKTKAFVDANEAHLEPASATQVDGRLLGSFQELQIRHATTVDELEHVRTQYLTTLRELDEMTAQHEEARRRVQSSSRLHQSSLASLTTSYSTPSTTSAASPSSPISFSPRRFPVHNGSSLLTNRRSRWQDYAVRPKKDVAEKEVLLQPIQVGDQAEQPRAQEEIYRNALQLSLEDSSHRSIPTRNSRNVPSTGSLKRGYSNSGTPSLQPLNLPLIRSSFSSIPQYSVLRGLMRSPNSPAPVSSVAEFSSPVPVNFANDQYTPHNAERSHESLEREVLQLQQVLKEREEEIRELELTIRQAPSLSELVDSPKSDHSVDPSGSNGYAHVSIPKISQNSNDRGCQLTASSRSHIRANKDDHHLSPALPCEVIETSQKSFSDINRNQPANFDNTESLNVLMRSMAKKESNFLETIGRLKSQLNSTERKHQDLVKLSADQVANMSSEIEALHERLKMSQAPNREVNPELLREQDLHAEQDRQAEIQALKDLQQLELEKVKMERSENFNQLFNFTNDKLMAKEEELRQLEIYWQAKLKAELTAQADLIWAQSDTQQSNLVRTQIKSFGAKASREREAFDREIEQLNKKHAEEIETLREGMNAKLKSAESDSANRIQELELMHAQEIAQVTSSLPSSMQKVEESSDGVSQAYVDEFQTWHTERNQLESRIHELENEKLYLISEHEMRLEQLKAEHATLITELQAENDDTLIGALSELDQRRTIKFQAQLKMAQENHVLKINEIKLFYEQKIEMINSEATQAYQSQSESKQTYPDLARSVVSPDVYSSTDPSNISVRVDSTTDTAILNPRMLKKIEDQESAISKLTKQLEQCEGNLRANISAIAQLEDALNDTERNLRKSRLQMNALVQERDKMSILNQRLTMGLNRSTEEVEHLKQSLQDEKLNLERRLSEEKLAKETARAQLETRMLEVQKSQEKKNKFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3C0</accession>
    <name>E3K3C0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_04933</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323396.1">
        <property type="nucleotide sequence ID" value="XM_003323348.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3C0"/>
    <dbReference type="STRING" id="418459.E3K3C0"/>
    <dbReference type="GeneID" id="10537165"/>
    <dbReference type="KEGG" id="pgr:PGTG_04933"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04933"/>
    <dbReference type="eggNOG" id="ENOG502S6K1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008138_2_0_1"/>
    <dbReference type="InParanoid" id="E3K3C0"/>
    <dbReference type="OMA" id="HENNYRA"/>
    <dbReference type="OrthoDB" id="107110at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266">
        <property type="entry name" value="CHROMOSOME 15, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266:SF1">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="814"/>
            <end position="841"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP78977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="862" mass="97620" checksum="831DD0E6802E46C1" modified="2011-01-11" version="1">MDSDREVVVSGPRSMAMSEAPESTTERTRFARLNPEIEQDIADRLFSTFVDIYGLTGMQKFNNQLETILGRLFQAYGEYGVDLWQDAIERLGDPTQAKRVKRVGIAQPAVYDELMRLAYSLRYPNEYAGNSLDEIFEALKLPIPPANTLNNHSKESLDFEWSAKYAQQGFQSDYLNSDNILDPVLETLRENATNWARDDYKAPFTAVIGPTMSGKTRLIMELAKKVPVVYICLRPEESTGLPSRSKLADYLLPEKRPTDLKLHYTNVLIAIFQAVADYFKSPEIVKRDQESQLTSWYEHSFPTDGKTDTGEFAAQVQVKMDTLQKLDGNKGFPALKRALADYSELRITQDDRLKIILAIDEARSMLKLKDGSEDSPFCVFRRVLSQIPSAHGFFALFTDTTSRVANFCPSLKYDQSARLHDLGWDLFAPIYQLQTLDLFAYKPKTWKEFVSPTRLMSYGAPFYGPYFRQAEMELGTIAAISQVIMIAQKKLLLTKSPDNMSQTQIFALLGPIIQPPLYTAWDLNAELLSSHAAHCMYISPARDRVVCQYPSQPIYATAAHRFLASDDKKWVTCINGLTLGVLQGLVSIGDAGELATNLILMRAINQTMEKVADKSTKRKNLPDTEDSILCDEKRNLIPYGHPVRLKDFLEVLTGKEADQIDLGSTKDELEMKNKLLNEGIIFFNHLILIKYTPNANDFLEYLHRGVAVQCKPRQPGFDQLFTIYLKPNSDTSNTSSLDVKNVSFCGIQVKNHEGSIKWAESSKWTTQYANIKDIKNPYLVILFNLSGNIPRRPKVVKQNDRKRVLLQIHGLGNIGCLTTEIASALDELKNTEADLLRMHKKDDQLQNFLKIINPHAYRTNTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3K463</accession>
    <name>E3K463_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_05705</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323803.1">
        <property type="nucleotide sequence ID" value="XM_003323755.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K463"/>
    <dbReference type="GeneID" id="10531208"/>
    <dbReference type="KEGG" id="pgr:PGTG_05705"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05705"/>
    <dbReference type="HOGENOM" id="CLU_2097988_0_0_1"/>
    <dbReference type="InParanoid" id="E3K463"/>
    <dbReference type="OrthoDB" id="2495499at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP79384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="135" mass="15088" checksum="26C58795B6885857" modified="2011-01-11" version="1">MNPPRYHVSHVWGLGRYSWMVVKPSGETIDVHFSMSSPAEASEAVGHRTLNLNKVCRALEATSGEGKEASKQLQRHARTTLSGNHQFLVDHPMRPLETQTSWMSFCKNSESFGALDSPIAKVINKVCTQQIHDRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3K4J6</accession>
    <name>E3K4J6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_05744</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79423.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323842.2">
        <property type="nucleotide sequence ID" value="XM_003323794.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K4J6"/>
    <dbReference type="GeneID" id="10531247"/>
    <dbReference type="KEGG" id="pgr:PGTG_05744"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05744"/>
    <dbReference type="HOGENOM" id="CLU_1504160_0_0_1"/>
    <dbReference type="InParanoid" id="E3K4J6"/>
    <dbReference type="OrthoDB" id="10471155at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP79423.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="179" mass="19553" checksum="FCA5F3B00091169A" modified="2012-04-18" version="2">MAIYVVGPCVAEVPDSLPVSSPGKPSDETARLSLKIISSAREWTAPNVPRKGKHALYDVHQGNARSSSPLDRDFRALGRPGFRLGIDQLQPWIEEALNVGSSGNRKRPLENEHILQQPHPSAVPVNRHVGPLQASSIDNSEKRHKAELLGSDGSCTTPDISRDHFDSNLSHTFDKNLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3K503</accession>
    <name>E3K503_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="5">AKL13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_05639</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323737.1">
        <property type="nucleotide sequence ID" value="XM_003323689.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K503"/>
    <dbReference type="GeneID" id="10531142"/>
    <dbReference type="KEGG" id="pgr:PGTG_05639"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05639"/>
    <dbReference type="HOGENOM" id="CLU_006433_0_0_1"/>
    <dbReference type="InParanoid" id="E3K503"/>
    <dbReference type="OrthoDB" id="301415at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.200.10">
        <property type="entry name" value="MHCK/EF2 kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004166">
        <property type="entry name" value="a-kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02816">
        <property type="entry name" value="Alpha_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51158">
        <property type="entry name" value="ALPHA_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Alpha-type protein kinase" evidence="4">
        <location>
            <begin position="390"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP79318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="689" mass="77070" checksum="5486BA25D6DD08CF" modified="2011-01-11" version="1">MTCIQCGNTPTVLGEYCLECVRSVKVKQQPAFQPTISPTPTSDAVTEAMRIKREEAINLARGLYGIPTPSAGSLSIPSSMKPPPPPPVHSNLVLPQNPDFPYASARAQRNSACKPYSTTRPRLVIKQSASGKGAIPLENPPYRVSVQCGFEVRHKNKWVSNQAPMRVATWIFPGDPNAHQALLLELTKRHEGKMQLQIKNYDGRLFFSEKHFPFCRLGTQKGSVIDQEGFIQFLKENKAIDLLVDYDEFEDHEEDEEEACGMETQSRQYALRSATSASFNSRANTPSVGSTLAISNDREDSTFSLPTITNKPPVITRENSPDIVVATSAHQIGLGSIKTPVDQAVAPVVIYKKSPPWLAPAILDLKDLSWDRFGTPEHSSTFRGHDDTGWANGLRSQLDPDGLYITLRICSYPEYTLVRKNLRQANESYETEPNYAMRTYAVSWQFMALFCKVIGSSKASSDERQLASTLRIIDGFPVHDDVECGKTNECFDYFQENDMIDGPGSGDGNGNNTSNIKEPLDNEKPRRVFFFQEKIKGYRTLLPPMANDYGISNASSPIDKILHAFQHWVYVSTKGQMIITNLKGNPPLITKPKVIDVNPRSYWYQCQDTKAMMAHFLKKHTCTNLCQVLGLPPLVEIVWTPTAKSLRPHQEYDLNNINHNSARPQMTSDQLELLAVVRQGQKALVSKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3K5L7</accession>
    <name>E3K5L7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="4">40S ribosomal protein S19-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_05795</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eS19 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323893.1">
        <property type="nucleotide sequence ID" value="XM_003323845.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K5L7"/>
    <dbReference type="FunCoup" id="E3K5L7">
        <property type="interactions" value="386"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K5L7"/>
    <dbReference type="GeneID" id="10531302"/>
    <dbReference type="KEGG" id="pgr:PGTG_05795"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05795"/>
    <dbReference type="eggNOG" id="KOG3411">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108559_0_0_1"/>
    <dbReference type="InParanoid" id="E3K5L7"/>
    <dbReference type="OMA" id="YYTRTAS"/>
    <dbReference type="OrthoDB" id="428974at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000118">
        <property type="entry name" value="40S ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001266">
        <property type="entry name" value="Ribosomal_eS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018277">
        <property type="entry name" value="Ribosomal_eS19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01090">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01413">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00628">
        <property type="entry name" value="RIBOSOMAL_S19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP79474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="150" mass="16716" checksum="61280D52F46B33A7" modified="2011-01-11" version="1">MPSVRDIAADAFIREYAGHLKRSGKIEVPTWVDIVKTGPCKELAPYDPDWYYVRAAGVARHIYLRKHVGVGALAKLHGTAKNRGNRPSHHVDASRSIERKILQGLEKIGVLEKDPRGGRRVSQDGQRDLDRIASNIAEQLRAGADEDDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3K5W8</accession>
    <name>E3K5W8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_05860</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79539.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323958.2">
        <property type="nucleotide sequence ID" value="XM_003323910.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K5W8"/>
    <dbReference type="GeneID" id="10531367"/>
    <dbReference type="KEGG" id="pgr:PGTG_05860"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05860"/>
    <dbReference type="HOGENOM" id="CLU_029800_0_0_1"/>
    <dbReference type="InParanoid" id="E3K5W8"/>
    <dbReference type="OrthoDB" id="2508994at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP79539.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="392" mass="45265" checksum="ECAFBA61B0C6717A" modified="2012-04-18" version="2">MPTITDLPMELLQHIFTDLVILHKRWPRSPGPPPTTTLRLVCRQWANWLYEKELYRTLYFTTRTKTLAFIGHIRQRSKLQLPRAKCAHLYAYLTYHESWHGLPEDEHEYRITSEMLESLVDLFSDTIVTLSLNFYEYVSLSTRTVAAIGRIENLLNLRLENELWHLPDERVTPFPRVTECFNSLLVTTQQLRSLRLGMPFSLPLGYGPAAGSQYPPITHLHLRLGAVKITDITTLSALLKHSLRVVTLCDLRVLEGFEPQLLLPFYENIRETIEGIGVSSPIALEPILGLRFPKLRVVAVQDWRDFLTDFLSRDLFLHSPIEVLALDSDAKRQVDPDPACNPFQNLLKLKRLVFMIIDYMSEISPRLRRAGEARGVKCVYLHHADMSVIMKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3K5Y8</accession>
    <name>E3K5Y8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_05880</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP79559.1"/>
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    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
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        <location>
            <begin position="107"/>
            <end position="136"/>
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    </feature>
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        <location>
            <begin position="207"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP79559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="288" mass="33550" checksum="7A60DD5A2BF77CD8" modified="2011-01-11" version="1">MITLCRYECEACHQHFGQDPSIYERHLLESGSHHYCKPCQREFGSAKALQAHLDQSQKHMCCRWCQSDVDNIRSHNQKYHTQCPRCKVWCANMEGLKEHCLQTHSDVYCAPCQTLFTQTNNLIMHLRSSLHQPKTVRCPHASCGKDFVSTSALIGHFEAGTCPSGVELEDVDEYFGHHCDPQQWFVRKELIFPQRVWQIPSDHPGPFQCPLCKKQFDHPGQIDYHLKSPKHKNYGRKPYICPSKRCDHATFYSLSSLLLHRETRTCEMGYRHELPKLLQRLFNIVAQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="2">Uncharacterized protein</fullName>
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        <name evidence="2" type="ORF">PGTG_05106</name>
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EFP79881.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003324252.2"/>
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    <dbReference type="KEGG" id="pgr:PGTG_05106"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05106"/>
    <dbReference type="HOGENOM" id="CLU_032241_1_1_1"/>
    <dbReference type="InParanoid" id="E3K6F9"/>
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    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        </location>
    </feature>
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        <location>
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        <location>
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        </location>
    </feature>
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            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="375"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79881.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="455" mass="50472" checksum="74B90C510C11677C" modified="2012-04-18" version="2">MSQEISEVRPDLPDLTIPAPLNQAADSSTTPKPKRNRRTNAEMEIYRASQGIEKSTSKKTNNEKEFKYWVPTARRLAALKKNSNAHRSAAKPATSTSRTADSNPQFIATDYQLICSYLEDPQHYTQIYGNSTRTSVGPQSLTKGAAYDVFAIYMNDQTNKRLHLDGKLLRQRIEAYKKRFTSAKHWADTTGAGIDICDNGITIKAKLESMCPCYERMDQIFGGKPNVTPAAQYESQGGMELYDQPMDDDRLRSTELFCSNREVNENQETLSAQDQPEDQSHIAAAGEETTMGGNAELPEEPNTRSTPALQAETRLSVQSQLLPHQLSIQTGTNQPRRTMASILGNLQRSVALDEAEDAADEVEDPPIEPGPSQGRFPNQNADNQSVPGPRREPTGNQKGKSTVAAAFKSSADKKFSYLDKHMAMEEPDAKLRMAEKLMSEGRSAAEIEIIMRCAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3K6G5</accession>
    <name>E3K6G5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05112</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EFP79887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324306.1">
        <property type="nucleotide sequence ID" value="XM_003324258.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K6G5"/>
    <dbReference type="GeneID" id="10533578"/>
    <dbReference type="KEGG" id="pgr:PGTG_05112"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05112"/>
    <dbReference type="HOGENOM" id="CLU_1797415_0_0_1"/>
    <dbReference type="InParanoid" id="E3K6G5"/>
    <dbReference type="OrthoDB" id="10468504at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="144" mass="16535" checksum="A25C7560D7FC347A" modified="2011-01-11" version="1">MRSERPRDALNKKASSGRLGNYPVDSFPENVRKLSGRVVSNGHSLPCKFWTPQMMSRRVSMGHQNSPVVYISVAWMMMTGEKKKKSANYEGRLPRRLCCSLGHSSGRTRHPFWTQHTEPRRSFPPREISWGRQFDKGRKDEGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAT0</accession>
    <name>E3KAT0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_06960</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325758.1">
        <property type="nucleotide sequence ID" value="XM_003325710.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KAT0"/>
    <dbReference type="GeneID" id="10536911"/>
    <dbReference type="KEGG" id="pgr:PGTG_06960"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06960"/>
    <dbReference type="eggNOG" id="ENOG502SUWC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040573_0_0_1"/>
    <dbReference type="InParanoid" id="E3KAT0"/>
    <dbReference type="OMA" id="CDIHEPA"/>
    <dbReference type="OrthoDB" id="2495729at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040223">
        <property type="entry name" value="PAR_bZIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988:SF27">
        <property type="entry name" value="GH27708P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11988">
        <property type="entry name" value="THYROTROPH EMBRYONIC FACTOR RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP81339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="524" mass="58927" checksum="95D69ECC48B2FF22" modified="2011-01-11" version="1">MAQLSDLPSAVVLRIINHLIRTPPDRRLNEVDHHDLDYKQIAPLLYEQRLRRPLEPGYIGTSRFLYDQGLPHPPSPPAGATFPDGLPKNPLLPSSLINRTFQRCAQQVLFRDVTLQTPWDAMLFLRSLDCPSSLDKPDLLAQNVRSIQFMWGGPCSLGKGGGYLICEILQRCPKIERIAIGTTLLVRCKDPVLDALASCSLVKEFVVLKNPIKGIPGWLANDISTRLLPNWDRLETIDFTRLCSRYDERKEPIPQPIPVINCALRKIILVQPDVYAEQLSLILKSSRESMQTLKIIDPHFKLTRAGLCQVLIDCAGPNLESLTIEINVGVNMTWDQIHYKDIIQGSDDPSKNRNLLDIVFRTSYAMSNLKSLTFAGSLASSDLFALLPPSIVKLAWERCHITGAAFAAALSSYQYYPSDDDLDDPYGPAQQVPLLPNLKCCSVRHAHDWEGDYKRAIQASFRARGACYHNKKYPSWCPYYDEGEPMELNNAYGLDHGDDYGCAGCVPSVDSDNGEFYDNQYEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>E3KB86</accession>
    <name>E3KB86_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_07848</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81599.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326018.2">
        <property type="nucleotide sequence ID" value="XM_003325970.2"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KB86"/>
    <dbReference type="GeneID" id="10532980"/>
    <dbReference type="KEGG" id="pgr:PGTG_07848"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07848"/>
    <dbReference type="eggNOG" id="ENOG502QT6K">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_257176_0_0_1"/>
    <dbReference type="InParanoid" id="E3KB86"/>
    <dbReference type="OrthoDB" id="10255630at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110085">
        <property type="term" value="C:mitotic actomyosin contractile ring"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032982">
        <property type="term" value="C:myosin filament"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016460">
        <property type="term" value="C:myosin II complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000146">
        <property type="term" value="F:microfilament motor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902404">
        <property type="term" value="P:mitotic actomyosin contractile ring contraction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1490">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18937">
        <property type="entry name" value="STRUCTURAL MAINTENANCE OF CHROMOSOMES SMC FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="221"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="324"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="405"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="627"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1283"/>
            <end position="1350"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="522"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="32"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="80"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="102"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="221"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="258"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="285"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="326"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="635"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1311"/>
            <end position="1350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP81599.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1418" mass="160686" checksum="2746A173428606DB" modified="2012-04-18" version="2">MADGVANDRAEKAARAKDRLKKFQAKKKAGEPSQTATTESNVTSEVASPSIEPVPNNQLHSTLPNGEQKIETIPPIPQPLDSQTGPSISEDTNGNHQITPDSLPSTSQLSESQQQTIALLVSEKSNLANQAEDYRQEISNLRNALLNAEQQSAEQGLKLNQDLEENRAALTRAQTDLEESTTSLSRLRTELEQARTQIEELSQSNESSKDEIIQEFESKLRMKQHHSDAVESELSRVKNQLKSQELRQQEQDQTIEQLQKDRTIAEERLKESTEKSREITASFDALQTNHQQLTAEMSSAQSQLKTSSEKFDALKSHADSLETRLSEFQTSHESLSSNVTQLEEKLEQSTQRKSALQEENQNLLQNLEELKTKIVELTNQKVQSSERCEEIRTIVRERENELVTMREEISSQRQSHEHEKERLEGDKKLLEEQLAESRRESDQVGLALKEEQSLLTHQLSSLTEQLEQQQSESARLSQQLQESQVLLEQSKEQLGLLADKQTQTDSELAEKIKFLEASAVLEQDLKAQLESLSSALSLEKNQKNELEQRVQQSQAELDQMQQHIKSLEGKLKDAAQQVEVHQERYGTQATQLKSLVSQNQEISDRLSLVTTESTSLKEQVVESQARISETEGRLQSKTSECDQLSQSMGHERSRVIELETLLSESKSKVESLMGRLEEARTGHDALIKEHAQQLANVENEGSRRLLEIQELQSQYADSQKRLKEIEDFRDGLAQQVSDLESELSRSESSAETLRASLESAKIAQEELRTESDVERKKLENLVSDYESKNLEQKDSLKKLEESVAELHLRLETAEQNLASSLKERQQVERELEESRSDLSEQRQNLASTLHDLVTAKNSLEDLENQLCAAKAENEEVVNSKEAVELELNDYREKMTTTLSKLQAELSSHKKQIDSQEERISQSEQQNAELQEEIGALEQEKNVLIKEMEAGKEGENRILEKSRSEVLELKQKIHQLKEEAERTKQDLSQTKAAFEQNDHVKEDEIHRLNNELKALKRTETELNKSREESLVEREEGIKKISELNQALESLQEEKKKVEKQFEERTQKSEGRLEDLTAQVSDLSAQLTENGHQQKEASSQALVAQEAAHQELARTTKQLEDLRAEFDTCKVEHQSNLDRSTRRTAELEEELEAAKMAQTQIRTEAENEQALLVESKTAELQRKLDKAEAELRGLSESQSATVSELRRESEGLRAELSATLEERKTAVDQTYVDSIIQQHQIDLSTARSQIRALESRVFEEESRSLEMLTRVENLQDELRGAEEALRQRKNTPLMTDSPNPAIPSSSSSTSSSAVPQTMVGSSSKTRPQSTSKKTNFSKMIDESNLSEQTKQTRTESLNLLKTRMENELGLLNLSSNALSHHPPDPSDAHSLKTSHKIIPRSKLGDDMVWCGHCVGDLFMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBF6</accession>
    <name>E3KBF6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07917</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326087.1">
        <property type="nucleotide sequence ID" value="XM_003326039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KBF6"/>
    <dbReference type="GeneID" id="10533213"/>
    <dbReference type="KEGG" id="pgr:PGTG_07917"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07917"/>
    <dbReference type="HOGENOM" id="CLU_1054251_0_0_1"/>
    <dbReference type="InParanoid" id="E3KBF6"/>
    <dbReference type="OrthoDB" id="10355598at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="75"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="264" mass="28721" checksum="8E77639194EECA24" modified="2011-01-11" version="1">MITSLLIIVHFRGSCKGVFHSPKEVISDGIAEIPMARDIAEQLTTPVHLTNPNPSLPVFPIPEVESQTATLDLASRRFSRSTSKDDRKRKISSDPAPDVRWNPPPQEPLILSVDEPRPHQVVKLMGIFLGPQVVPSSSEGNGIVEQSRLQITAPLPVTMKGINSVNQDVLRVSQAPLGSATASNLPGIPCAISASNPIRLGTHVLSIEDSSANAIKENSQGRNQGPSDSSRLTFDKELFTFFARSKKSSKLLWKLKTIENPNLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBF8</accession>
    <name>E3KBF8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_07919</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81670.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326089.2">
        <property type="nucleotide sequence ID" value="XM_003326041.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KBF8"/>
    <dbReference type="GeneID" id="10533215"/>
    <dbReference type="KEGG" id="pgr:PGTG_07919"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07919"/>
    <dbReference type="HOGENOM" id="CLU_061626_1_0_1"/>
    <dbReference type="InParanoid" id="E3KBF8"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24383:SF20">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24383">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="199"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="284"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="45"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="199"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="303"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP81670.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="329" mass="36695" checksum="BC421B6CB0CBD522" modified="2012-04-18" version="2">MNQLMRQRPVNNTDMPYMNYQPPNKPGHHRQTQTNNQNQTPLHSPEADHPSIEAVKHNKPDLQGPEDYNECPNDLDEHVNNLSEQEQAYKTISDNHDPEVLPEIVPESDDPQVTEPAFSMDNYSARDVNCWARTLSDDQFEQLRILGPASRIESFRQYAITNLDQPTQTFTQIMSSTPQSNQEPLSQIPSEVADNHNQYVDSTSNETNNPFLHSHHVNHQPYQSTHSSYYDCSNYNITDLDENPYDTVVHEDGVLDDGSFGDGGPDNCGFDTGKFDDGGFDDGGINDGGFDDGGFDNGGFDDGGFDDGGFDDGGFDEGGFDDGYGSPYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBN2</accession>
    <name>E3KBN2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07952</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326122.1">
        <property type="nucleotide sequence ID" value="XM_003326074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KBN2"/>
    <dbReference type="GeneID" id="10533410"/>
    <dbReference type="KEGG" id="pgr:PGTG_07952"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07952"/>
    <dbReference type="HOGENOM" id="CLU_1750600_0_0_1"/>
    <dbReference type="InParanoid" id="E3KBN2"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="149" mass="16565" checksum="13136FA48EB18CAC" modified="2011-01-11" version="1">MCTVGAPIFLARGNLGYKLVMHWLVIFVVIGVVNKVNHSNQSSSPHKTSLPPWNNQPDHHQSIWSEPQMQAPLVTQAEGKAERSTIATAPRSGQLRMTNDQDLCELEKVLNKNPWMKMAEVKDILTTPISTKTTLCNSTGHLTHMELLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KC46</accession>
    <name>E3KC46_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_08071</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EFP81822.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326241.2">
        <property type="nucleotide sequence ID" value="XM_003326193.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KC46"/>
    <dbReference type="GeneID" id="10533693"/>
    <dbReference type="KEGG" id="pgr:PGTG_08071"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08071"/>
    <dbReference type="HOGENOM" id="CLU_1230408_0_0_1"/>
    <dbReference type="InParanoid" id="E3KC46"/>
    <dbReference type="OrthoDB" id="2507674at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171898" evidence="2">
        <location>
            <begin position="33"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="108"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP81822.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="207" mass="20787" checksum="87B62FC5FE7F62EA" modified="2012-04-18" version="2">MTMSFGNTLTKRGGISLLFLCLCISISGPVQTQDVTDPATSATIPATSPVGPATSSPAASSVVPTGATQDPTATTAGKTADTKPTLDSPTTLPQPGLTPPVTGGATVTMPTTSAGTSLNSTNTSPVPAGPGGFTAPLQDGNGKKIVFPITDTNGRVIFQSATDMHISGMKGANSTSPSTKTPAGSSDSKGDKNVRWSLIYEIERSFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCH6</accession>
    <name>E3KCH6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_08173</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EFP81924.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326343.2">
        <property type="nucleotide sequence ID" value="XM_003326295.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCH6"/>
    <dbReference type="STRING" id="418459.E3KCH6"/>
    <dbReference type="GeneID" id="10534124"/>
    <dbReference type="KEGG" id="pgr:PGTG_08173"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08173"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_650764_0_0_1"/>
    <dbReference type="InParanoid" id="E3KCH6"/>
    <dbReference type="OrthoDB" id="8922241at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="entry name" value="GDNF-inducible zinc finger protein 1"/>
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        <property type="entry name" value="Zinc finger protein 574"/>
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        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24394:SF44">
        <property type="entry name" value="ZINC FINGER PROTEIN 271-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="253"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="12">
        <location>
            <begin position="281"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="93"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="324"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="25"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP81924.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="422" mass="47030" checksum="9864ECD2CC05E579" modified="2012-04-18" version="2">MASAARQANFTEENLQYADDIKPTLQPTDSNPQSFLYPAYPALSPTPTKPEPSPVKYEDIQPKHEVKYEPQVEYEPSDNYPFLAFASSSITAAPSSPPVETASPHSAHGSHITPSSESFSCPLPAQVQLPPLLSSRPEYPQSTWLYQPTYDQSRSPTTEVFYQRERGDLSHCLRLNHRTSSHAHRLSGPYATYAPEYGYPAASDYHSLNEFGYNECRPNTHRILYPQAIYPGNGTSPYSHPVPHSTTAPDRPFVCEKCGARFNRNHDLKRHTRIHLEVKPFPCGWCEKAFSRKDALKRHLMVIACSGSKEVSVEESVRRAEQVRQRKFESPSPYTANTPPAPLLDKKANSPCNTKLEKPFHSISPTPKSHHGSATVHSQGPQKIHASPIQDSHFHHYVARDRKCSLSADSVSTNGSATTCSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCQ7</accession>
    <name>E3KCQ7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_07606</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326628.1">
        <property type="nucleotide sequence ID" value="XM_003326580.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCQ7"/>
    <dbReference type="GeneID" id="10529581"/>
    <dbReference type="KEGG" id="pgr:PGTG_07606"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07606"/>
    <dbReference type="HOGENOM" id="CLU_053739_0_0_1"/>
    <dbReference type="InParanoid" id="E3KCQ7"/>
    <dbReference type="OrthoDB" id="2507984at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172534" evidence="2">
        <location>
            <begin position="22"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP82209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="422" mass="48011" checksum="2642D073E3D510CF" modified="2011-01-11" version="1">MPSTLLSFITIAVYSCTLCQAPPPLNPFAGEGGVGHTFAQGPGPFYNGIGLPIEYGNLAPGHHLNNVVPRFPVEDFPEHLAPSPSQAPHNYYYNRDREDPDGFRRSHYTNGQSSGNPPRLPFPFQQVPPIHSPQPWIKFGGSHVHPGAPNSIKNAHDHFHGRPSLERVAIEHSSAITTGRRTPLSSETRQTWKRQGESYRALNKDDRQEVNFWLGQWFLNTHPSTEDWNEISAYVWSLQESVRTTSRPTAVWWKQSLIDGNKPNNQFIQLVQIGDRLKFFDPSPALQLNGVEAEEITKDITTILYLGKDRQLRQNALNVEALTKYRDAGSPQVSMYEARVGQLMRHRLRDFDSQNDVEQTRRLRELYLFYGVTSTKADRLSPKALSKKGHKSFGKLFEFQAYDYPGQTIYNNIQTQLFKLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCV9</accession>
    <name>E3KCV9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07642</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326664.1">
        <property type="nucleotide sequence ID" value="XM_003326616.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCV9"/>
    <dbReference type="GeneID" id="10529618"/>
    <dbReference type="KEGG" id="pgr:PGTG_07642"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07642"/>
    <dbReference type="HOGENOM" id="CLU_044507_0_0_1"/>
    <dbReference type="InParanoid" id="E3KCV9"/>
    <dbReference type="OrthoDB" id="2509616at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="483" mass="53668" checksum="DB1439B5E3DB9C2F" modified="2011-01-11" version="1">MSSPHDMDTSEEDERPAGDGDASTSQGGRGGAANADDDEFDRMLGGGGEDHEDDEDSDSGIEDPTAALKHQVEREKRKRAHENSQANTRKVRKAVVGQQLPSQSGGLSRSIRNFIKFMMGMPASLSAYPAPPTKKEWDTWDNWTKNRYDKVTAHLKSFTEKNKEVAKPTLKKMYRDEMDRIRKHLTPPSFKPSGDVLNSAINIPIHVKKSCEQEIQLAGFGRLTFEWTARSFTASPWNGTLGTILIKHYYQWAKSQAGTLWDETSNMEKILDRWVQGQGKLMKKGDRSDGKSLDDLKREKARKASISRGKTKIAEKRRDVAMKKMGNQPSLALLFTKETISDWEDQPAPAKAKRIALQWRSAAFTMCAIKLDEMALTICKTTAEKAATRKLLERDPVSIQGVAVEFDMVPRKLPLDAYDPTFLLGLSPIEKEHLDAKEAIDLDAKVQTLNEMTQRGGTGMAQMARQKFGDPGLGGVPGPSSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDB1</accession>
    <name>E3KDB1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_07523</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326545.1">
        <property type="nucleotide sequence ID" value="XM_003326497.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDB1"/>
    <dbReference type="GeneID" id="10534657"/>
    <dbReference type="KEGG" id="pgr:PGTG_07523"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07523"/>
    <dbReference type="HOGENOM" id="CLU_514009_0_0_1"/>
    <dbReference type="InParanoid" id="E3KDB1"/>
    <dbReference type="OMA" id="RTERIHG"/>
    <dbReference type="OrthoDB" id="2503055at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="261"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="485"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="516"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP82126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="565" mass="61784" checksum="842AE822133E6E5D" modified="2011-01-11" version="1">MDDSTTVTTFDDELDHPPTKTSAKHLSSTDDDPGAATIVSAASLDPANMGTPSSTPTGLGARPAWKSKPTGGSAGIPKESFSPPPVAVSSKLAASSNVSVNSNVSVSPSVAVSSVSPLPSPETLPIRSIEVEAPPRQHNSVGVVVAAILSVSVVLVLVLVVGYLTSQRYRRWRESRRARYTETKWKISRPLPEGESLRSSMREVPQIEISTNELVYAPLDPFASSIPRQSRMPFNLGSSDPVEEIDEERGWLWGTQTSKKKGTDRLVTPGLGVGKYRSKNSRGQRQDSLNSRQSHLQTDDIDELMEELMYADEAGDAYKYDNGPEEPSTRFATTGVSSLLGRLKDSFSDRSGFGSLSSSAGRLGIDSKRGRWNEVGRLVIDDEKDITTQDDDDHEKTPGFQVRFENNVSFRSASPLSEILSTSTWDNHSDHKVAKINRARAPSLEGEIDDAQAGYISTGHPFRQPDACPRTSGTNSVRQLVNRLEENEGKFTHLPSPSSRQPRRKRKEALPSRYDTQPDQHTHASKIRTEKLGGLVKVKSKHLYPGGYVGRSPTKKLRKKDSPDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDE8</accession>
    <name>E3KDE8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">sphingolipid 4-desaturase</fullName>
            <ecNumber evidence="3">1.14.19.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_07554</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the fatty acid desaturase type 1 family. DEGS subfamily.</text>
    </comment>
    <dbReference type="EC" id="1.14.19.17" evidence="3"/>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82157.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326576.2">
        <property type="nucleotide sequence ID" value="XM_003326528.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDE8"/>
    <dbReference type="FunCoup" id="E3KDE8">
        <property type="interactions" value="84"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KDE8"/>
    <dbReference type="GeneID" id="10529529"/>
    <dbReference type="KEGG" id="pgr:PGTG_07554"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07554"/>
    <dbReference type="eggNOG" id="KOG2987">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_032156_1_0_1"/>
    <dbReference type="InParanoid" id="E3KDE8"/>
    <dbReference type="OrthoDB" id="200948at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042284">
        <property type="term" value="F:sphingolipid delta-4 desaturase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046513">
        <property type="term" value="P:ceramide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03508">
        <property type="entry name" value="Delta4-sphingolipid-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011388">
        <property type="entry name" value="DES1/DES2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013866">
        <property type="entry name" value="Sphingolipid_d4-desaturase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12879">
        <property type="entry name" value="SPHINGOLIPID DELTA 4 DESATURASE/C-4 HYDROXYLASE PROTEIN DES2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12879:SF8">
        <property type="entry name" value="SPHINGOLIPID DELTA(4)-DESATURASE DES1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08557">
        <property type="entry name" value="Lipid_DES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01269">
        <property type="entry name" value="Lipid_DES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="159"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="189"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="276"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="327"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Sphingolipid delta4-desaturase N-terminal" evidence="11">
        <location>
            <begin position="119"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="26"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="63"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="75"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="88"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP82157.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="464" mass="52499" checksum="04C02E0AABF9336B" modified="2012-04-18" version="2">MDSEDYRVWSRSEIVGDEVDWRTARNIHPRTTIKKEEMTAPSKPDTSINWSLLAGAETTKPPSSSSSSSSSRSSADLSTPNTSVLADASPPSSFASVSSEKLAPSKAQPWMPKSQGLSQDPNDWLWQMTEEPHRSRRRAILKDHPEVRKLMGYTWTTKWVTLFVVSLQLSVAMYFGSRSKEGLESAWNWKFFVAAYVIGGTANQNLFLAIHEITHNLAFKKILHNRLLAIFANLPIGIPYAMVFKRYHLEHHKALGEDGIDTDLPTKLELLCLRNVLGKTFFATFQILFYALRPGFVRSQKLTGWIVASFAIQLGFDYVLYKHFGLASVMYLIVSSFMAGSLHPCAAHFIAEHYVWTADGGDQETWSYYGPLNMLAYNVGYHNEHHDFPSIPWTNLPELNKIAHEYYDPLPKHSSWPMVIIRFIFDNNVGLFSRVKRVPGGPHLPLVPAPDSSVNHTVEDRKDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDQ3</accession>
    <name>E3KDQ3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82489.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326908.2">
        <property type="nucleotide sequence ID" value="XM_003326860.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDQ3"/>
    <dbReference type="GeneID" id="10529868"/>
    <dbReference type="KEGG" id="pgr:PGTG_08445"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08445"/>
    <dbReference type="HOGENOM" id="CLU_1272821_0_0_1"/>
    <dbReference type="InParanoid" id="E3KDQ3"/>
    <dbReference type="OrthoDB" id="10294752at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172558" evidence="1">
        <location>
            <begin position="23"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82489.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="217" mass="23481" checksum="82FFBC740D331BD5" modified="2012-04-18" version="2">MDACKFFLILISLLAHSFLVSSQASCTNQYHLSLADCTTARNSIKFVNGDNGKGVVGKGELEIAVGRGTCMIQILNSVEGVKQADIYSAIDDILKTCAPGYDGTSQPPTFVARVTKNLIGTDPINQPKCNNTTPSVIKTEDCKVAIDRIGLDKSTGLLTQDKNFGINSVYKTCQVRVNSAFTTIPINVNKDVLKTSVQKITTVCQNKLSRKMEAEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3KFX6</accession>
    <name>E3KFX6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_09092</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EFP83139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327558.1">
        <property type="nucleotide sequence ID" value="XM_003327510.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KFX6"/>
    <dbReference type="GeneID" id="10544852"/>
    <dbReference type="KEGG" id="pgr:PGTG_09092"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09092"/>
    <dbReference type="HOGENOM" id="CLU_1949873_0_0_1"/>
    <dbReference type="InParanoid" id="E3KFX6"/>
    <dbReference type="OrthoDB" id="10286533at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP83139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="129" mass="13948" checksum="5B4645C7802F156B" modified="2011-01-11" version="1">MNITPPPRKLRGIVVLKLTALLFGALMVLAMIAISQVSADQLFDCSDLPGKSPFCGTAHRNRDLVTVIPAPPVPSPSTPGQPYSCQDNSKKISNGTPVCCVPKTARQISKAEFRQKCSRETKFIPDGIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHP4</accession>
    <name>E3KHP4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eukaryotic translation initiation factor 4E type 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_09532</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic initiation factor 4E family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83819.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328238.2">
        <property type="nucleotide sequence ID" value="XM_003328190.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHP4"/>
    <dbReference type="STRING" id="418459.E3KHP4"/>
    <dbReference type="GeneID" id="10532506"/>
    <dbReference type="KEGG" id="pgr:PGTG_09532"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09532"/>
    <dbReference type="eggNOG" id="KOG1670">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023472_0_0_1"/>
    <dbReference type="InParanoid" id="E3KHP4"/>
    <dbReference type="OrthoDB" id="2505571at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016281">
        <property type="term" value="C:eukaryotic translation initiation factor 4F complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000340">
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        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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    </dbReference>
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        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Chromosome 7, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.760.10">
        <property type="entry name" value="RNA Cap, Translation Initiation Factor Eif4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023398">
        <property type="entry name" value="TIF_eIF4e-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001040">
        <property type="entry name" value="TIF_eIF_4E"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 4E RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11960:SF73">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 4E, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01652">
        <property type="entry name" value="IF4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55418">
        <property type="entry name" value="eIF4e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="1" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="170"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="470"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="553"/>
            <end position="603"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="656"/>
            <end position="719"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="51"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="93"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="227"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="473"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="553"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="672"/>
            <end position="701"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU004374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP83819.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="719" mass="75068" checksum="C67E35CD97BF9946" modified="2012-04-18" version="2">MDRASPPSSADGRIGKQTLTPPSLLELTEAMRSNPERWQQLKIETDSERLTTNTTNTTTTNSASTPTPSTATGTGRLRLPLSTLRTSLDRRSSISASTPTTTTNSPPDPQPQTNSNITTTTTSTTDPNQSADSQHHQLLLQRRGSASDINIGINRARAPSLAAIQERIARMKPDALPSKTEAKPTSSEKPASATDPPPALVLTSTSSSSSNQIIDSQPKDAQPKPPSQTVGEAVQQQPISSNDQAVNQQPNTDHQPSQPLQELHPLQHSWTLYFDTRLSKRSSTSGNHATGGQAYEAGLQPIGTFQSVEQFCRFFNWTVIPSQMDMNSSVQLFKAHIKPMWEDPANSKGGKWTITMKSNSNLALLDKLWTYLVLGLVGEQIETSSTSPQQQQQQQDEDLVCGAIVATRPRGNRIQIWVKEKDNVEKINSLGKRLINVLEINDHSGVSVEFSAHSGGSHGASKFISIQGHGGHVSNTSSPQTRGGNHSQTPLIRPAIEGMVIGGMGEKGLGGGGGIGLMRRSTSAEVSQLAAGRTGGTMGVIPAGGPAGFGGAGSFRGIPGGPTAGGRGGSQLSSLDPPIQNGVWRPAPAGQPPSTLARPSAVQAGPPGMMAAFIANNRRPQSALNLPTIGSLSSSSSSSAANSASPAALPAFATLNPASKSRHPSPAPGLEPTSNNTTTTASTTPNPTTTTSSSSSSNPTRNPSPAPPAPLGLGILSGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHP7</accession>
    <name>E3KHP7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09535</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83822.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328241.2">
        <property type="nucleotide sequence ID" value="XM_003328193.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHP7"/>
    <dbReference type="GeneID" id="10532509"/>
    <dbReference type="KEGG" id="pgr:PGTG_09535"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09535"/>
    <dbReference type="HOGENOM" id="CLU_159481_0_0_1"/>
    <dbReference type="InParanoid" id="E3KHP7"/>
    <dbReference type="OrthoDB" id="2507923at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173400" evidence="1">
        <location>
            <begin position="22"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83822.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="102" mass="11223" checksum="69664C953162A9DD" modified="2012-04-18" version="2">MHVFKVALMMMAISVDRLATAYDCGKDAPQNVCEVSHGDLAIYVRADKIPVRLDKNLSFPAGGKNCVEYGPHTRRCCPLATIPAGSWISSDRYKQAKCHKAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHT9</accession>
    <name>E3KHT9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_09577</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EFP83864.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328283.2">
        <property type="nucleotide sequence ID" value="XM_003328235.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHT9"/>
    <dbReference type="STRING" id="418459.E3KHT9"/>
    <dbReference type="GeneID" id="10532553"/>
    <dbReference type="KEGG" id="pgr:PGTG_09577"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09577"/>
    <dbReference type="eggNOG" id="KOG1305">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009020_4_1_1"/>
    <dbReference type="InParanoid" id="E3KHT9"/>
    <dbReference type="OrthoDB" id="2496167at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015179">
        <property type="term" value="F:L-amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003333">
        <property type="term" value="P:amino acid transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF458">
        <property type="entry name" value="SODIUM-COUPLED NEUTRAL AMINO ACID TRANSPORTER 11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="178"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="221"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="300"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="334"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="376"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="416"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="460"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="482"/>
            <end position="499"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="520"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="10">
        <location>
            <begin position="148"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="100"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="66"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP83864.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="555" mass="60423" checksum="D442AC0BCD391668" modified="2012-04-18" version="2">MDEPTDRECLLSSEDRSFEAPDIPSPEPTSSQRAQQKAGLSYPPRLASTIASRESAFEADSDTVSEFDSTRNTPTRGEGLDPSSELRMTGLLEAGQRRGSVMTTGDDRPSYMFSDDIEEPLLPQDRSKIGGPSRQESDDYSHLLDSGTGMMAGVANMANSILGAGIIGLPYALRNAGFLTGTVMVIVLGIVTDWSIRLIALNSKMTGQRSYIGILEQCFGFPGKAAVSFFQFIFAFGGMCAFGVIVGDTIPPVLSTLFPFVPKSQWFSFLFSRSFVITFFTITVSYPLSLYRDIGKLSKASAMALMSMVMIVFSVALGGPSVDPKLKGNPSARWTFIQPGIVEAIGVISFAFVCHHNSLLIYGSLRTPTLDRFAQVTHVSTALSVFACLVMSFSGFLTFTDRTEANILNNFPRDDLVINIARVCFGLNMFTTLPLECFVCRETIDTFFYPDEMFNLRRHVIHTTLLVGIGMLLSLWTCDLGVVLELTGGLAASALAYVFPAACQLKLSSKTGSIFERENWAGLLTVAFGLAVMLISTITSLSKALDPHRVPKVCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHU1</accession>
    <name>E3KHU1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09579</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EFP83866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328285.1">
        <property type="nucleotide sequence ID" value="XM_003328237.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHU1"/>
    <dbReference type="GeneID" id="10532555"/>
    <dbReference type="KEGG" id="pgr:PGTG_09579"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09579"/>
    <dbReference type="HOGENOM" id="CLU_2005012_0_0_1"/>
    <dbReference type="InParanoid" id="E3KHU1"/>
    <dbReference type="OrthoDB" id="2495146at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172630" evidence="1">
        <location>
            <begin position="23"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="123" mass="13684" checksum="5B7AFC03BD38EDB9" modified="2011-01-11" version="1">MQFSSFFKLCTLLLIQSGAVYSTFVCNDNNIEDAKVGICLRKINTAQDPTDPTVSPLLDKKDFLARDARGTDIQYHFTCEGVTIGKGPVEGRFCCKIETSTAIRGLTHEEVNRLCYSRDGKPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KI69</accession>
    <name>E3KI69_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09707</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EFP83994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328413.1">
        <property type="nucleotide sequence ID" value="XM_003328365.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KI69"/>
    <dbReference type="GeneID" id="10532687"/>
    <dbReference type="KEGG" id="pgr:PGTG_09707"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09707"/>
    <dbReference type="HOGENOM" id="CLU_1074159_0_0_1"/>
    <dbReference type="InParanoid" id="E3KI69"/>
    <dbReference type="OrthoDB" id="10274338at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172019" evidence="1">
        <location>
            <begin position="19"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="259" mass="30134" checksum="231D41E2F4546E1E" modified="2011-01-11" version="1">MSFRFLILMSQIISVTYALEMLRMRISSPQHEMYRVDYATSDQVNNKEYDSMWLETEKIHDTFGRSEIGQQALDLLKTLKTHDGDSPTRILVVDKIADWEKFWLERERPAATFHIFDSADNDLRKFINKAFSKHLPNGIPYMRILPLKDRIWIDYNTEIPNSMGYLTDWIPLETALESFKGISDQGEKAIQLIEHYAKLNTENAKSKTKIDFSAILVDLGDNGWEELDRFNSERGAYYSHVSVEDPKVVTLINKTLCQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KI77</accession>
    <name>E3KI77_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09715</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EFP84002.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328421.2">
        <property type="nucleotide sequence ID" value="XM_003328373.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KI77"/>
    <dbReference type="GeneID" id="10532697"/>
    <dbReference type="KEGG" id="pgr:PGTG_09715"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09715"/>
    <dbReference type="HOGENOM" id="CLU_091094_0_0_1"/>
    <dbReference type="InParanoid" id="E3KI77"/>
    <dbReference type="OrthoDB" id="10549055at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="165"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="113"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84002.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="281" mass="31159" checksum="D73A91AD915FD488" modified="2012-04-18" version="2">MTFALPPDWRKGIKRSIRQLKMQESQSFLEFSTQARTLRLMLNFDDEDAFGEFELAEYVTFGLSDELQGKGSDFRLLRIKPFDYGDFEERVAGFFEHLPKRSSGRPRPVTGASQDRSTSPQSSSLWRLHSYLESLGRCHFCKKNCGSENGACPGQIDKSYVEIPSSFKTPPKPADYKPPKATTSQNTTAGKPTHQPAGRPLQRAANVAGVEEINYFPELSTAAIAALDEHLLGLEASDTADNIQPAQVDGITLEDLGPEELEALAAIDEELQLAKDERSTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJJ7</accession>
    <name>E3KJJ7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10897</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329157.1">
        <property type="nucleotide sequence ID" value="XM_003329109.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJJ7"/>
    <dbReference type="STRING" id="418459.E3KJJ7"/>
    <dbReference type="GeneID" id="10526706"/>
    <dbReference type="KEGG" id="pgr:PGTG_10897"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10897"/>
    <dbReference type="HOGENOM" id="CLU_1611589_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJJ7"/>
    <dbReference type="OrthoDB" id="548867at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036871">
        <property type="entry name" value="PX_dom_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="65"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="165" mass="18493" checksum="D2DC3387B5425275" modified="2011-01-11" version="1">MGQKGTQTTLRLRCFSKKIFILCLMRKLVGHITFTLGSWHGAGGDPSAYNHSKQSSANTPWNAPTNSSSNSQQSLTQQGQGCQDKPYPPQSGPKGDGYATVEELREQHGVVVHASVESFHFDYWFHLRAHFSRISEDGRDGQTTILALYCLYEDFYDFQATLIDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>E3KKG0</accession>
    <name>E3KKG0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_10256</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="DS178292">
        <property type="protein sequence ID" value="EFP84785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329204.1">
        <property type="nucleotide sequence ID" value="XM_003329156.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KKG0"/>
    <dbReference type="FunCoup" id="E3KKG0">
        <property type="interactions" value="55"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KKG0"/>
    <dbReference type="GeneID" id="10526757"/>
    <dbReference type="KEGG" id="pgr:PGTG_10256"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10256"/>
    <dbReference type="eggNOG" id="KOG0605">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_67_4_1"/>
    <dbReference type="InParanoid" id="E3KKG0"/>
    <dbReference type="OMA" id="KLRVDQF"/>
    <dbReference type="OrthoDB" id="18472at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034973">
        <property type="term" value="C:Sid2-Mob1 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007096">
        <property type="term" value="P:regulation of exit from mitosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd21776">
        <property type="entry name" value="MobB_Sid2p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000141">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000319">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000109">
        <property type="entry name" value="Non-specific serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000961">
        <property type="entry name" value="AGC-kinase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050236">
        <property type="entry name" value="Ser_Thr_kinase_AGC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356:SF417">
        <property type="entry name" value="CELL CYCLE PROTEIN KINASE DBF2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24356">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51285">
        <property type="entry name" value="AGC_KINASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="243"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="domain" description="AGC-kinase C-terminal" evidence="13">
        <location>
            <begin position="546"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="87"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EFP84785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="667" mass="75122" checksum="CA3E099AB10784A6" modified="2011-01-11" version="1">MSSSSSTPQSQQTTTTTTTTQQKPPPPPTDQDYQSRETAKMVPFPINTPQLFTPSSKPNTTSTNEPANPRNALTPLKDTNNRLERMQQQQPAAGQSQPAGHKPFVHPPPSVEPTMSELLAASANIPGIPAFNNDGAGFMSDVRGDNGLVSWRKGQGFRAWESAILANPEVQRKANVAQLYFYDHYFDLLTYLNGRKKRLALFRETSTRPEIDPGQAGMEWRSYCGRERALLRKRRTKLKLDSFHIIVQVGQGGYGEVYLARHKLSKEVVALKKMKKKTLLKMDEVRHVLTERDILTATSKSDWLVKLLYAFQDPDYIYLAMEFVPGGDVRTLLNNSGVLKEEHAKFYATEMFVAVDSLHRLGYIHRDLKPENFLIDRSGHIKLTDFGLAAGSINPGKIQSLKHRLDEAKDENLQLWRSTVEMKTIYKTLRLQEPRYADSIVGSPDYMAIEVLRGQSYSFSVDYWSLGCILFEFLSGYAPFAGSSPEETWANLKNWSKVLRRPHYDKPEDQIFNLSDEAWLAIVALINAKEKRCSSIEQVKKLGFFADLQWDDMRQVGPPFIPSLDSEIDAGYFDDFGNPEEMKKYGEVFEKARAVELVQERAEAGGPHKNNCRGVWVGFTFKGGKTAELLRGAVPSDLPPNLAPSSAASLANPFREFGDDDVLECMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLZ8</accession>
    <name>E3KLZ8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hydrophobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11516</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178294">
        <property type="protein sequence ID" value="EFP85347.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329766.2">
        <property type="nucleotide sequence ID" value="XM_003329718.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLZ8"/>
    <dbReference type="GeneID" id="10543890"/>
    <dbReference type="KEGG" id="pgr:PGTG_11516"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11516"/>
    <dbReference type="HOGENOM" id="CLU_1338094_0_0_1"/>
    <dbReference type="InParanoid" id="E3KLZ8"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172074" description="Hydrophobin" evidence="1">
        <location>
            <begin position="22"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85347.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="22949" checksum="DB4795C759AF43B0" modified="2012-04-18" version="2">MNLIKLTTALIINTACGLVTSEVINFYTNEIRSFGCDPSEVALCIVTRYTRPNDRNSRPDYYLLDTAVRVKPGTYNCGVTTAKCCGTNLKYEKWQHVKDSQWMKDLTETFCPNKYACDPDTPTKRSQPGYLVVYFIVELVVVHTDLHSIVQSKGAGIGLMLDKNQDSFLCDDNRTRPGGNLPVVETLLSLTTRSNPPGLKERGRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3KMU3</accession>
    <name>E3KMU3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_11121</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal/bacterial/fungal opsin family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EFP85792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330211.1">
        <property type="nucleotide sequence ID" value="XM_003330163.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KMU3"/>
    <dbReference type="STRING" id="418459.E3KMU3"/>
    <dbReference type="GeneID" id="10544367"/>
    <dbReference type="KEGG" id="pgr:PGTG_11121"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11121"/>
    <dbReference type="HOGENOM" id="CLU_1147656_0_0_1"/>
    <dbReference type="InParanoid" id="E3KMU3"/>
    <dbReference type="OMA" id="ILWTAYP"/>
    <dbReference type="OrthoDB" id="10261467at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009881">
        <property type="term" value="F:photoreceptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007602">
        <property type="term" value="P:phototransduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1070.10:FF:000965">
        <property type="entry name" value="Gll0198 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1070.10">
        <property type="entry name" value="Rhodopsin 7-helix transmembrane proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001425">
        <property type="entry name" value="Arc/bac/fun_rhodopsins"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28286">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28286:SF2">
        <property type="entry name" value="BACTERIORHODOPSIN _OPSIN, NOPA (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01036">
        <property type="entry name" value="Bac_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00251">
        <property type="entry name" value="BACTRLOPSIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01021">
        <property type="entry name" value="Bac_rhodopsin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81321">
        <property type="entry name" value="Family A G protein-coupled receptor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0157">Chromophore</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0600">Photoreceptor protein</keyword>
    <keyword evidence="10" id="KW-0675">Receptor</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0681">Retinal protein</keyword>
    <keyword evidence="4" id="KW-0716">Sensory transduction</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="37"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="72"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="121"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="188"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP85792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="242" mass="27213" checksum="46067BFB93A4BE05" modified="2011-01-11" version="1">MSSVPTTKILWVTFGLMTISTAAQWVLTTRQQPNTKLLHHISLTLSTLTLVTYYFILTDINVTGSSIWIRDLHWILTLPLVLVELGLISGVSIQDLVIMALNGIGMGVTGFLGTIQRSNRLRWPLVLMAWIFWLSIVREILTSGRRSSARNSVRVARFFNYLAAYIVLLVSFYPIIWSAPVDPFLEIALFSILDMLTRPIFATWILIGVSIIPESNVVGANRYIEARIWNSIGGNIEEPPPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KN94</accession>
    <name>E3KN94_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11059</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EFP85730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330149.1">
        <property type="nucleotide sequence ID" value="XM_003330101.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KN94"/>
    <dbReference type="GeneID" id="10544296"/>
    <dbReference type="KEGG" id="pgr:PGTG_11059"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11059"/>
    <dbReference type="HOGENOM" id="CLU_877548_0_0_1"/>
    <dbReference type="InParanoid" id="E3KN94"/>
    <dbReference type="OrthoDB" id="2507848at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="248"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="317" mass="33901" checksum="7BACDBBBA6082977" modified="2011-01-11" version="1">MSASSIRDIGGSSSNNTPGALPPWTTDPSPRIYRVDPANNSPLNGSDFKPPSHDDLILPTVARQIEKQTKLLTISPTEHPTFSSVSSNFSQWEALGPQLLLRKASHKRIDGSFAQSSTLSVDEPLSSPPTHDEELVEPVSPTSGPRVGPTGGAGPGRRMSRGMSGSQGSEGGRGGGSSRRRSSYLSPTSPGRRGSQQSFSSHTNSSRASPSAANRLVLGPAPAPSSHQPSAPDQALDHPFASPVLLRPLSFQQQQQQQQPQPQQSLPPPDQLDPSFPPHRATSPNTHNQLDDDNLHRFDQVRHLDPEPEGCCKCIIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNJ5</accession>
    <name>E3KNJ5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_11626</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178297">
        <property type="protein sequence ID" value="EFP85870.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330289.2">
        <property type="nucleotide sequence ID" value="XM_003330241.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNJ5"/>
    <dbReference type="GeneID" id="10544452"/>
    <dbReference type="KEGG" id="pgr:PGTG_11626"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11626"/>
    <dbReference type="HOGENOM" id="CLU_1027246_0_0_1"/>
    <dbReference type="InParanoid" id="E3KNJ5"/>
    <dbReference type="OrthoDB" id="2504034at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="158"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="183"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP85870.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="271" mass="30085" checksum="191652D711263BB3" modified="2012-04-18" version="2">MSLTSYIPAYKNLREMESIINDLTSTLREEANAYDSEKLRGFSASSESSLLQSAQKLGPLRVELAKNLRRGLFIYCIDTCLLVVVGQTLAQFQGLYEGSAPPENVEAAPSLIKRLKKNPLRRKLNRDRRRLVCHALCVYASTALHLPPLIILGLNDPLAVLANVILLILNINSRQLIRARMEASRLNPQPPARSPDPNTEQRKAISNSLNDRSSQRCSPPPPDIHIALNNLEENVPYNEKTASWCTCAAPGDLDRKSPLVISDPHANETDC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPI2</accession>
    <name>E3KPI2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12173</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178299">
        <property type="protein sequence ID" value="EFP86217.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330636.2">
        <property type="nucleotide sequence ID" value="XM_003330588.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KPI2"/>
    <dbReference type="GeneID" id="10540227"/>
    <dbReference type="KEGG" id="pgr:PGTG_12173"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12173"/>
    <dbReference type="HOGENOM" id="CLU_434847_0_0_1"/>
    <dbReference type="InParanoid" id="E3KPI2"/>
    <dbReference type="OrthoDB" id="2499263at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="519"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="538"/>
            <end position="554"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86217.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="629" mass="70864" checksum="17F6EFA91D26DFFD" modified="2012-04-18" version="2">MDLNRAGSAISHGLNDFSAPSETEPSMSSLGSVFHSGEKSASPSQGNEPISEIGAIKSETEDFKDKQSNALDNWKKLSSKILDFKTRAEHTGASFQEYLREQQGITTYLGRIDVLVSEIFEDTIPHPTQDPDRAHILITKPIFELKTFLEKDFLPFLQLKHDESVSKVLHRHELWLQEHQSKLNLKLQQLQECLHLLREEGGMEEIEIKLVAAVLGVLDMSYQHALIDKEEVQNFFNDERIIKIVGEHLVYLYGEKYPNFSNPANFKPDMDYLLRDPRTSHLHACLEMLNRPTQGKIIYRSIQTAFADTRPNEGASDGNLFFMNACTGFLDDSFLEEFEWRESSESSEIKHDLGPKLEIVYTHLARIYNELPPDINQLEFLTVLHFLNFFMTYNANNIEKLIYSNFKGFKDKYDLISRGLKIHELRNVIFSKDRPAASNALIGFTSPTISHAILERSIGAVSPAEYDQQLKKMKSDAQAKTWLESEIVSSVLEQIHQSRMGLISPEEVKKNEWLQDFKKDPSNALTSSPSETNEDRVQSSNHADVPTESSSDSHVSGFGAGRIMSNVYQGGASVIKSVSEGGSGTISFVARGGVHKSVLNGAKYVLQSEVGSTALAVPRDYENHSHCFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQ83</accession>
    <name>E3KQ83_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="3">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_12414</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclophilin-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="3"/>
    <dbReference type="EMBL" id="DS178300">
        <property type="protein sequence ID" value="EFP86458.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330877.2">
        <property type="nucleotide sequence ID" value="XM_003330829.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQ83"/>
    <dbReference type="FunCoup" id="E3KQ83">
        <property type="interactions" value="813"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KQ83"/>
    <dbReference type="GeneID" id="10540473"/>
    <dbReference type="KEGG" id="pgr:PGTG_12414"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12414"/>
    <dbReference type="HOGENOM" id="CLU_012062_31_2_1"/>
    <dbReference type="InParanoid" id="E3KQ83"/>
    <dbReference type="OrthoDB" id="10264753at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01927">
        <property type="entry name" value="cyclophilin_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000003">
        <property type="entry name" value="Peptidylprolyl isomerase domain and WD repeat-containing 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000450">
        <property type="entry name" value="Peptidylprolyl isomerase domain and WD-repeat protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF4">
        <property type="entry name" value="PEPTIDYLPROLYL ISOMERASE DOMAIN AND WD REPEAT-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0697">Rotamase</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="9">
        <location>
            <begin position="513"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="13"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP86458.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="659" mass="73296" checksum="86472BA7772A8A03" modified="2012-04-18" version="2">MSEETARKRSRTESLSPKPTDPNPTTNEESDEEVGPLPMGNDEKGLKKMEKTRKKARAAHQHERLYLKNLPSAERYHRSFMHRDVVSWITVTKTLFIITASVDGHIKFWTKKDPKEGPGIEFVKHYRAHLSPIVSVSASVDGMMYASVSAEGEGGSIKIFDVKNFDMINMFKINFVPQACCWLHDIGQAATILAVSDAQSPAIHFFDGRGDGKPFMSTSTLHQAPVHLFAFNPRFNCVISADTSGMLEYWEPSEGFALPEGLKWSLKSQTDLYSFKKSKSVPSSLTVSDDGSYFATTTLNSTEHSICVFNFSSGKLLRKYDESITAITEMQQAGTAAYSLDDMEFGRRLAVERELGKTVESGLMPGACGSAVFDPSSTFLLYPSLLGIKIVNIQLNKMVRILGKDENHRFLHLSLLPSLLRKEEIMTVAMAISDNPLLADEGRTEPSLFCTAFKRPRFYIFNQTEPESDSGTKNTDRDIFNEKPTREEQAVAAVAPAAKVLLGTSAVIHTTRGDIHCRLNPELVPKTVENFVGHARNGYYDGVIFHRIIRKFMIQTGDPRGDGTGGESIWGGNFEDEFVPNLKHDRPYCLSMANAGPGTNGSQFFITTVPTPWLDNKHTIFGRCVSGFDVVHEIENVRCDKTDKPLPDHEVKITSITVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQH2</accession>
    <name>E3KQH2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Helicase ATP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_12929</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178301">
        <property type="protein sequence ID" value="EFP86547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330966.1">
        <property type="nucleotide sequence ID" value="XM_003330918.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQH2"/>
    <dbReference type="STRING" id="418459.E3KQH2"/>
    <dbReference type="GeneID" id="10541132"/>
    <dbReference type="KEGG" id="pgr:PGTG_12929"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12929"/>
    <dbReference type="HOGENOM" id="CLU_839735_0_0_1"/>
    <dbReference type="InParanoid" id="E3KQH2"/>
    <dbReference type="OrthoDB" id="10261556at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003000">
        <property type="entry name" value="ATP-dependent DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF153">
        <property type="entry name" value="RECQ-LIKE DNA HELICASE BLM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="60"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP86547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="331" mass="36679" checksum="5C30A23FA62EA74C" modified="2011-01-11" version="1">MGALNPDIRADADIRSQFQRKYTSASAGGYPPALAGIRADTPPSEWAARLFQEVDPALCLVYVTPERIMRSSVLSNILTDLKNRKLLARFVFDEAHCVSQWGHDFRPDYKGIGPMLRIEFKNIPFIALTATANHRVQQDVTSNLKITGCRVLTQSFNRINLRYEVRPKTKDVLNDIIQIITVDHKGEIGIIYCLSKKQFEEVATHLSAKNRIKAHHYHATIGTSSSGGVPPDELVLNQLAGRKRSQRVGLWTLAGRNPSRRAGLKTTGGIPPGGVASWHRVSQGIPGYPPVFQGEGCIRTPIRTRWQVSAGTGGYPPADSGYPRQIIRDHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQJ3</accession>
    <name>E3KQJ3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12950</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178301">
        <property type="protein sequence ID" value="EFP86568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330987.1">
        <property type="nucleotide sequence ID" value="XM_003330939.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQJ3"/>
    <dbReference type="GeneID" id="10540667"/>
    <dbReference type="KEGG" id="pgr:PGTG_12950"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12950"/>
    <dbReference type="HOGENOM" id="CLU_903550_0_0_1"/>
    <dbReference type="InParanoid" id="E3KQJ3"/>
    <dbReference type="OrthoDB" id="2506969at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="251"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="251"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="277"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="308" mass="33977" checksum="C9E6EF6CA3C83B39" modified="2011-01-11" version="1">MTSVTRTHPIPSPSIDHNPLKQAVTKTWNGFGLDKQVIKNLEKLSLVPEEIHKRIILSILKADHLEIIVPPDSHTTTAIQAALISYLITSSSNDHTPAPTQTSPMVLILCMNTHSVERHTLVCKQLGTDIGALDLEDINHSECLSTDLEIARRSVDSPIWITTLPRAGLLLQQQALSLNRLSILLLDLRHSNHLNHHTLAELEPLINSLGPHVQLIFLISLNTRVSPALSQSITQKMESSIRINLSNQNTPALTPTSLSPTPSHSVSQIPSPPSYVTSTNSIKVNTQDDYQAMTTFNDQLKTHHKPNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQW1</accession>
    <name>E3KQW1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Polyubiquitin-A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_13068</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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    <dbReference type="EMBL" id="DS178301">
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13068"/>
    <dbReference type="eggNOG" id="KOG0001">
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    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR10666">
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    </dbReference>
    <dbReference type="Pfam" id="PF00240">
        <property type="entry name" value="ubiquitin"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="UBQ"/>
        <property type="match status" value="8"/>
    </dbReference>
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        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00299">
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    </dbReference>
    <dbReference type="PROSITE" id="PS50053">
        <property type="entry name" value="UBIQUITIN_2"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="6">
        <location>
            <begin position="77"/>
            <end position="152"/>
        </location>
    </feature>
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        <location>
            <begin position="153"/>
            <end position="228"/>
        </location>
    </feature>
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        <location>
            <begin position="229"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="6">
        <location>
            <begin position="305"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="6">
        <location>
            <begin position="381"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="6">
        <location>
            <begin position="457"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="domain" description="Ubiquitin-like" evidence="6">
        <location>
            <begin position="533"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP86686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="609" mass="68185" checksum="F8299190058FAC05" modified="2011-01-11" version="1">MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3KRJ5</accession>
    <name>E3KRJ5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_12661</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178303">
        <property type="protein sequence ID" value="EFP86920.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331339.2">
        <property type="nucleotide sequence ID" value="XM_003331291.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KRJ5"/>
    <dbReference type="GeneID" id="10541672"/>
    <dbReference type="KEGG" id="pgr:PGTG_12661"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12661"/>
    <dbReference type="HOGENOM" id="CLU_055675_0_0_1"/>
    <dbReference type="InParanoid" id="E3KRJ5"/>
    <dbReference type="OrthoDB" id="2507634at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="277"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="46"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="277"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="298"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP86920.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="334" mass="33925" checksum="357AAA8FCC691C7D" modified="2012-04-18" version="2">MASPQAASPALASSQAASPAATASPKVSTPTKSTAPAPVVPPISNPPVTSAPAPSSPQATTVPIPISPPNTDSSIQHASVSQAAKSVPTSVASPNSVQPAPSISTSRPKSITGTPANAPVADANVDTPSSPDTLSDAPSGSKSVVGPVIGSIFAVLLVIIAVVGGVWYLQRRREKARVRHEAKRMRSSNFGLYETTGDVVQEKQASIEATLEADLARLRSLDANIPIIVDDYDSKYPERSSISQQPQPVPSLQIARKPSYLAKNSYKVASSNIAGSRYAADSSPSAPAYVESTESKDYGGGGAGGAGGGGGVCHSTTGAFPSFDTPASSLAEKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3KRZ3</accession>
    <name>E3KRZ3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRAL-TRIO domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_13287</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
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    <dbReference type="KEGG" id="pgr:PGTG_13287"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13287"/>
    <dbReference type="eggNOG" id="KOG1470">
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    <dbReference type="OrthoDB" id="43460at2759"/>
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        <property type="entry name" value="SEC14"/>
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    </dbReference>
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        <property type="entry name" value="CRAL-TRIO lipid binding domain"/>
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        <property type="entry name" value="CRAL-TRIO_dom"/>
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        <property type="entry name" value="PHOSPHATIDYLINOSITOL TRANSFER PROTEIN CSR1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="CRAL/TRIO N-terminal domain"/>
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    </dbReference>
    <dbReference type="PROSITE" id="PS50191">
        <property type="entry name" value="CRAL_TRIO"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
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    <feature type="domain" description="CRAL-TRIO" evidence="2">
        <location>
            <begin position="192"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="84"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP87068.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="528" mass="59897" checksum="C4B80F9B48861EA2" modified="2012-04-18" version="2">MSGTTQQGNPLPENGLVGKLTASQYGALKSFWREFFRLVDSAPEKGSAEETLNDDSQEPLPSSSSGLVDQQEPELDLKDDPQLSNNRSAGPSSNPGSSGNTKDEHLKEKLRAEQELRDAKAALERYGKTRFLDTFWNMIMMDDPDVIVLKFLRARKWNVAAGVAMMAACMKWRIEYGVDSIIAKGEEGFQNQDGFLHQLKIGKTFVQGTDRQGRPVVYINVRFHKASDQSPKTLEEFIVFSMESVRLMLTPPLIEKVTIIIDMSGFGLANMDWKSLAFIVKCLESYYPESLNVLVVHNPPWVFQGLWKIIAPMLDPVVRAKIQITKSTEELKEHIEEGHLLSSLGGTNGWRWEYEGVSPDENVLMADTVVRNHELQNRKKLIKAHLDITRKWADEAGPAEVLRDDLLVPDDSSPSALIRKFITYHLRVHYFVLDPYIRGRSVYHRKGRVIGNGLITFEYPQLRLSKQTDHPIDPTPLSNNNSHKEGDDTVLVDSWEVLGYPMSRENLLIQIEKMKRSFALNNIDFPVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KSR8</accession>
    <name>E3KSR8_PUCGT</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12785</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178305">
        <property type="protein sequence ID" value="EFP87201.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003331572.2"/>
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    <dbReference type="KEGG" id="pgr:PGTG_12785"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12785"/>
    <dbReference type="HOGENOM" id="CLU_004591_4_2_1"/>
    <dbReference type="InParanoid" id="E3KSR8"/>
    <dbReference type="OrthoDB" id="10321256at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87201.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="155" mass="17405" checksum="F19896E57976B91E" modified="2012-04-18" version="2">MKYECWLCKGRTTTNQKRHTMNQRHQNLVQMNKDQVAQRASQRAAVEASTNETLIMDGVSNLLEPVIGMNEGQPDQVHADESELDEESEEIPPGTFVEEESDDKEAELSWMDFVGVAIGQIDSEPEDLDLPSSSFVAKKKKSRRSDPTTPPGTHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>E3KU88</accession>
    <name>E3KU88_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Kinase</fullName>
            <ecNumber evidence="4">2.7.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_14578</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the inositol phosphokinase (IPK) family.</text>
    </comment>
    <dbReference type="EC" id="2.7.-.-" evidence="4"/>
    <dbReference type="EMBL" id="DS178309">
        <property type="protein sequence ID" value="EFP87863.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332282.2">
        <property type="nucleotide sequence ID" value="XM_003332234.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KU88"/>
    <dbReference type="FunCoup" id="E3KU88">
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    </dbReference>
    <dbReference type="STRING" id="418459.E3KU88"/>
    <dbReference type="GeneID" id="10534762"/>
    <dbReference type="KEGG" id="pgr:PGTG_14578"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14578"/>
    <dbReference type="eggNOG" id="KOG1620">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_290542_0_0_1"/>
    <dbReference type="InParanoid" id="E3KU88"/>
    <dbReference type="OrthoDB" id="2573163at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000824">
        <property type="term" value="F:inositol-1,4,5,6-tetrakisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008440">
        <property type="term" value="F:inositol-1,4,5-trisphosphate 3-kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0032958">
        <property type="term" value="P:inositol phosphate biosynthetic process"/>
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        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
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    </dbReference>
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        <property type="entry name" value="Inositol polyphosphate kinase"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="IPK"/>
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        <property type="entry name" value="IPK_sf"/>
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    <dbReference type="PANTHER" id="PTHR12400">
        <property type="entry name" value="INOSITOL POLYPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="KINASE"/>
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    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
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        </location>
    </feature>
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        </location>
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        </location>
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        </location>
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    </feature>
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        </location>
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            <begin position="1"/>
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        </location>
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            <begin position="953"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007374"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU363090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP87863.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1053" mass="118511" checksum="DC91F772AC9A4C31" modified="2012-04-18" version="2">MTTNSPSTTTTSRKASVSLQLFKETNNNNNNSSPPQSPKNNEQLNPITTSPSSSSSFNPISSSPSSTRPSSSSTHNNKNKRTQSENNNHHIQPSPHRTCSTSIRLIARNQLTPLDLESSDHHEIGRQELTSTSRNHSSNNEYNNESRSQLSSTISNHHHHHHHHHHQFIPFNTTTLQSNSSSFVSSPNQANQFELHSDSELTHPTLPPKINQPSRNSSLSSSTSSLSSCEDDNHFKDSDNIRLVNDHISHTVPLEPFDNQVGGHHSIFRFSRRAVCKPLVSRENTFYEAVERDHPELLAFVPQYLGVLNVTYRRINHSPQSASSTDDNSHRPPIQSRSSNLSQRQVFRNKDGRNRLGDANQDEIPEVTLSQNRHILPDSSLWNRMDFLSSQPFDSHPSSDHHPERSFHHHHPSSSTHSPGLLDEIGFPSDHTTSSLVLSDHHPHPLDIVSANKNLRNSPLTPHSTPVGSPHGCSIWNENGQLGTVPNEKDPLSSYFRPPGSGSSSLSAIRGRGSTQVNRKLCEQVLREVFSSPKLPLNGQLHKPPWRNGRRTSRLKDSIDSTRETTTEISTPPRSPDCLRRRSYLLPHDPHPEDSPVSSLDHQDSLGRPEVRRTMSDSQVNRERFSVVHHENPSNHHPQTTIDDQYHQHPPASSSQSAPHCSMENDSQYSDLPHLVEPPRSNFDSQEHPSELVDTLQFTSIVSSTNLHPTKKSQELHPPSTKHHKQPHKHHHQQQQQQLQQRQQLQQQLQAQETIQYPLRQEQFLLMEDLTGNLLCPCVLDLKMGTRQYGIDASPEKKISQTSKCKQTTSGNLGVRICGMQVYKVSENRYTFQDKYFGRKVTTADFVGTLTEFFHDGERILSHQIPGILRKLYQLARIVSKLNRYRFYASSLLFIYDGDKHTQLEYERGLINQQQGKEKGEKLEGHRGEVNIRLIDFAHCTTGDDFISPSSPTHEDDPERPRASFPPSHPNQPDCGFLLGLKSLCAALKEIWELQRNSDGDDGLEHGEGGKHSLPPLDVSGADVWEVIFGPGADNCGVGQEFEMHAFASLLTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3KWX2</accession>
    <name>E3KWX2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_14204</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178317">
        <property type="protein sequence ID" value="EFP88865.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333284.2">
        <property type="nucleotide sequence ID" value="XM_003333236.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KWX2"/>
    <dbReference type="GeneID" id="10541981"/>
    <dbReference type="KEGG" id="pgr:PGTG_14204"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14204"/>
    <dbReference type="eggNOG" id="ENOG502S45T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067862_2_0_1"/>
    <dbReference type="InParanoid" id="E3KWX2"/>
    <dbReference type="OrthoDB" id="2103474at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025187">
        <property type="entry name" value="DUF4112"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35519">
        <property type="entry name" value="MEMBRANE PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35519:SF2">
        <property type="entry name" value="PH DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13430">
        <property type="entry name" value="DUF4112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="122"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="208"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP88865.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="244" mass="26732" checksum="E33B720540C295D6" modified="2012-04-18" version="2">MTDKIAAKVARSFFKGQATAFEPRDPLYETSVNPQTGKRKRTQRQLPPGLSKHEEKILKKIRKRAHYLDKGFSICGFRFGWTFLIGFIPVVGDAADAALSYYLVIRPARTCNLPALLVQRMFLNQIVATSIGLVPIVGDIIMAIWKVNSRNAALFEDFITLRAQHAVDASQPNGVTPSETAALAENSTRTAYIDGITGDREPLASGLNPGQSSSAGPSQAANNSENTDANKIRKPSQGWFKLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3KXY0</accession>
    <name>E3KXY0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ribophorin II</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Ribophorin-2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGTG_15022</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endoplasmic reticulum membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the SWP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178320">
        <property type="protein sequence ID" value="EFP89181.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333600.2">
        <property type="nucleotide sequence ID" value="XM_003333552.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KXY0"/>
    <dbReference type="STRING" id="418459.E3KXY0"/>
    <dbReference type="GeneID" id="10546736"/>
    <dbReference type="KEGG" id="pgr:PGTG_15022"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15022"/>
    <dbReference type="eggNOG" id="KOG2447">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051361_1_0_1"/>
    <dbReference type="InParanoid" id="E3KXY0"/>
    <dbReference type="OrthoDB" id="432292at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055374">
        <property type="entry name" value="Ribophorin_II_3rd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056790">
        <property type="entry name" value="Ribophorin_II_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008814">
        <property type="entry name" value="Swp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12640:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12640">
        <property type="entry name" value="RIBOPHORIN II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23860">
        <property type="entry name" value="Ribophorin_II_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25147">
        <property type="entry name" value="Ribophorin_II_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0732">Signal</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="13">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044264945" description="Ribophorin II" evidence="13">
        <location>
            <begin position="29"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="257"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="281"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Ribophorin II third" evidence="14">
        <location>
            <begin position="39"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="Ribophorin II C-terminal" evidence="15">
        <location>
            <begin position="205"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF23860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF25147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EFP89181.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="328" mass="36220" checksum="4515A99CA1213B0D" modified="2012-04-18" version="2">MARITRTVGQPFLLALAILALWLPSGSAELVVDNGQLVVLDVNGMTKKSFEISTNAPSPLAPPFELNDENTLKATFEILEKSSSDQAGSLFSPHQVTLLATGVDTKLHWAAAVKTRSKGKAKWELDLARAPTDFLSLSRTGEVRLELIIGDISAVHAPLKLNLATFKIPKSLLLEYPYWDTKDGKPPSTLELEKHYIQPELKWTFQPPRKKDNPIFSLAFVGIVASPWLFLLTAWAKISQNTTGGFKLFGAPKLSTLLFILTMVSQEALILTYWARLKLYQYIPLAFLMSFPLILCGRTALSELRLRRKISPISAARVSSSRGSAGDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KYA3</accession>
    <name>E3KYA3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15473</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178321">
        <property type="protein sequence ID" value="EFP89294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333713.1">
        <property type="nucleotide sequence ID" value="XM_003333665.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KYA3"/>
    <dbReference type="GeneID" id="10547014"/>
    <dbReference type="KEGG" id="pgr:PGTG_15473"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15473"/>
    <dbReference type="HOGENOM" id="CLU_152095_0_0_1"/>
    <dbReference type="InParanoid" id="E3KYA3"/>
    <dbReference type="OrthoDB" id="10346601at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173847" evidence="1">
        <location>
            <begin position="22"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="102" mass="11260" checksum="2F8E96061EED081C" modified="2011-01-11" version="1">MYPIPSLVFLILTTSLGLITAVEEQRDARHPFKCTGIHPVGYCGQKQGYKVYSKHHLTCADTGMKTNWCCKNGLEFMPGTSSVSQNHIYGDKGSCVYGRTAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3L0B8</accession>
    <name>E3L0B8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16185</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178327">
        <property type="protein sequence ID" value="EFP89897.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334316.2">
        <property type="nucleotide sequence ID" value="XM_003334268.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L0B8"/>
    <dbReference type="STRING" id="418459.E3L0B8"/>
    <dbReference type="GeneID" id="10545711"/>
    <dbReference type="KEGG" id="pgr:PGTG_16185"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16185"/>
    <dbReference type="HOGENOM" id="CLU_860897_0_0_1"/>
    <dbReference type="InParanoid" id="E3L0B8"/>
    <dbReference type="OrthoDB" id="336088at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.50.10">
        <property type="entry name" value="Erythroid Transcription Factor GATA-1, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029617">
        <property type="entry name" value="Snt2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013088">
        <property type="entry name" value="Znf_NHR/GATA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47672">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SNT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47672:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE SNT2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89897.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="323" mass="35497" checksum="7D2ACD5D1D364DC0" modified="2012-04-18" version="2">MVTALVQPGNGPDGVLRTVDGWPFRYYGRFTEPASVLDPMDSPHIYTAPRVGSRHQTMVPVNPSIETGNASSSIQSAGRPVKADKRSRDGTPVNVSCFASTEVDTPRGGDETIDVISTPDLLEKYQDSDLNDYIRDAAQLPVARHVGVTILDGALKTLSEVSSLEEARLKLSEKTIKNLGFVNWTEGDSDKVDKVVSQLGDDLRAIRKAFPKKSGGDITKFFYMFKGHKFPEVSAHDARIDASISHSPDESSCLLPTPSSKRPYTCVVCEVRSAPTWRRCPEALMAGATQAKKSICDECYVRWKKYGLQHVPVAEQEDNHRGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>E3L256</accession>
    <name>E3L256_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Helicase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_16657</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178335">
        <property type="protein sequence ID" value="EFP90631.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335050.2">
        <property type="nucleotide sequence ID" value="XM_003335002.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L256"/>
    <dbReference type="STRING" id="418459.E3L256"/>
    <dbReference type="GeneID" id="10530531"/>
    <dbReference type="KEGG" id="pgr:PGTG_16657"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16657"/>
    <dbReference type="eggNOG" id="KOG0385">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000315_17_3_1"/>
    <dbReference type="InParanoid" id="E3L256"/>
    <dbReference type="OrthoDB" id="5857104at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140750">
        <property type="term" value="F:nucleosome array spacer activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031507">
        <property type="term" value="P:heterochromatin formation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18009">
        <property type="entry name" value="DEXHc_HELLS_SMARCA6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10810:FF:000063">
        <property type="entry name" value="Chromosome 12, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001315">
        <property type="entry name" value="SNF2 family helicase/ATPase PasG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044753">
        <property type="entry name" value="HELLS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10799">
        <property type="entry name" value="SNF2/RAD54 HELICASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="228"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="712"/>
            <end position="872"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="86"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="304"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="536"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="117"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="304"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP90631.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="966" mass="108346" checksum="566ECE121E9693C6" modified="2012-04-18" version="2">MATPSIESSTLDSGLPTQTTSVEGTPTMQTDTQTKDPAAPPFQEITLEGNEKPESREKLARLNYLLKASGVYSKIIADNIERERASRAAALKKQADHLKKQESESKKQDLESKKPDPTQTKQGSSPDQKTAERRSSRRTKADCPAPKPNHPTKAKSSGTQSRRKKQVIADENEDDTEAEIVKLLENVEPDNSEATIPTEKHDPQSGQPALITGATLRSYQLAGMHWLATLYENGLNGILADEMGLGKTLQTIAFLCYLRERGVWGPFLIVCPLSTVANWVNEFERFCPTVPVVLYHGTKAEREELRSSRMAPPRDDPKAWLGKAAGSSRRPSSKDKRGGRAATTKWTNHNTKDTFPIVVTSYEIVMNDCQFLRQYAWSYIVVDESHRLKNLECKLVQELKSYQSANRLLLTGTPLHNNLKELWSLLNFILPEVFDDYSSFEQWFDLSSVTAASDSAAALSTAQAQHIVTSLHAILKPFLLRRVKSEVETNLPKKKEYLLTAPLTAQQKELYDAVINRDLRTYLVHQKTQAGLLNKAENLPDPELRKKRSRTSSPELENDTEVQKVKRSRTVETLADDCPSEEDTPIANLAKGPKVRKLARRAAATRKSVGYTEKSDSQFFKEVEAGECDEIDYTWDESRPKSKADGAENITQEEQVGLLAQKSVNQMKLLNMVMQLRKVCNHPWLFDWPIDPKTGEQLVGEGLVSASGKMLLLDRLLKGLFDRGHKVLVFSQFTSMLDIIQDWATELKGWRVSRIDGVTRQESRREQMKEFNEGEGPDACRLFLLSTRAGGVGINLVAADTVILFDSDWNPQQDLQAQDRVHRIGQTKPVLVFRFVSGNTIESKMLEKAGQKRKLETLVIGNGFDLTQKAEDDDANSDDDIQNILLGKQSNRRKQKTMKELAEALLKTDGERITLIEQGDQILGDDQLDALLDRSDEAMERKLGWKTEASSAVPTGVEVYQTKAPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3L2Q2</accession>
    <name>E3L2Q2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycerophosphoryl diester phosphodiesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_16829</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178337">
        <property type="protein sequence ID" value="EFP90803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335222.1">
        <property type="nucleotide sequence ID" value="XM_003335174.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L2Q2"/>
    <dbReference type="FunCoup" id="E3L2Q2">
        <property type="interactions" value="97"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L2Q2"/>
    <dbReference type="GeneID" id="10530707"/>
    <dbReference type="KEGG" id="pgr:PGTG_16829"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16829"/>
    <dbReference type="eggNOG" id="KOG2258">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030006_1_0_1"/>
    <dbReference type="InParanoid" id="E3L2Q2"/>
    <dbReference type="OMA" id="ECSWEYL"/>
    <dbReference type="OrthoDB" id="1058301at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030395">
        <property type="entry name" value="GP_PDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43805">
        <property type="entry name" value="GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43805:SF1">
        <property type="entry name" value="GP-PDE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03009">
        <property type="entry name" value="GDPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51704">
        <property type="entry name" value="GP_PDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GP-PDE" evidence="2">
        <location>
            <begin position="40"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP90803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="335" mass="37489" checksum="086AAC6137A7A133" modified="2011-01-11" version="1">MPATQHQPLSDDSLQSSGLPITDDQDTGTAAVNKPNNGLPLCWGHRGASATFPENTLCSFEAAIKDGAEGIESDVHVTADDVVLMFHDPSLERTTDGQGMIGSLPYVDGIDAFRTLKAPHQKIPTFDETMDLLMLKENQHVVFNIDCKPNNDPERLFRLIKASIERFEDHATLLSPRLVLGLWHPKFLDPSARHLPDLRKVYIGSSPLTARKYFWEACQGFSMKFSCLVGWEGLSFREDCQKAGKSLLVWTVNDRQEMIEACKWGVDAILTDKTADYLALRQQMKDDWSAVNAETSALFPYSSIHYNGLVSWLRGRVESYVLSRSVGPFHPMTTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3L350</accession>
    <name>E3L350_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17247</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178339">
        <property type="protein sequence ID" value="EFP90975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335394.1">
        <property type="nucleotide sequence ID" value="XM_003335346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L350"/>
    <dbReference type="STRING" id="418459.E3L350"/>
    <dbReference type="GeneID" id="10528390"/>
    <dbReference type="KEGG" id="pgr:PGTG_17247"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17247"/>
    <dbReference type="eggNOG" id="KOG4178">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020336_20_1_1"/>
    <dbReference type="InParanoid" id="E3L350"/>
    <dbReference type="OMA" id="MSLMEMI"/>
    <dbReference type="OrthoDB" id="19657at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050471">
        <property type="entry name" value="AB_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43433:SF5">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43433">
        <property type="entry name" value="HYDROLASE, ALPHA/BETA FOLD FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="33"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="299" mass="33615" checksum="7B45182F998EE29B" modified="2011-01-11" version="1">MPTKSSHQPRHIYYEVHGDLKASQKIVLVMGMNFTCSAWASQVQYFSKKKDHVVLVFDNRGTGNSNAGAIEAYRTSDMAQDTVTLLNWLGWNQDRSLHLFGMSLGGMIAQELCLIVPKRFKSATFISTRCGSELDWPSKGAWEVLVKTSAKKANGDEGLDLYLNVLFPKEYMETVVEQDTMLKSDLREKLRNCHRLPREQAPIPFMGHFYAATMHHCPHPKLHRIAADLKPAKMLILTGGSDELIPTKRSLELHKNLPGSELVVLENAGHALIFQLSEEVNTIMERAIVEGNQAFKLVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3G6</accession>
    <name>E3L3G6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16953</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178341">
        <property type="protein sequence ID" value="EFP91091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335510.1">
        <property type="nucleotide sequence ID" value="XM_003335462.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L3G6"/>
    <dbReference type="GeneID" id="10528670"/>
    <dbReference type="KEGG" id="pgr:PGTG_16953"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16953"/>
    <dbReference type="HOGENOM" id="CLU_046021_0_0_1"/>
    <dbReference type="InParanoid" id="E3L3G6"/>
    <dbReference type="OrthoDB" id="2506077at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="149"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="289"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="318" mass="36329" checksum="2301618EC9A4EABC" modified="2011-01-11" version="1">MATIHFFQKDFNKSYLSTTTIALSLSTYPFMLCTVWNVLRALPLTKYGLVNAHSTKTYFKPRIINLLSIFFYAFPFVFAGPQVFLITLYTTEITQIYNEHNNSFLAIISGNLEAATIYQLNIEASARIASIQERGEKVLILSRLISFGYWLYILALFLMMLFGHLFVLQAVRYQLRTFRQVLQPQIPLTLSILQLAEARPELRPTPASAPFKTPSGAYTIETQRKTPPALGSFSRWLPSLRHDQNFLEQSVSSNSFDDSGLKNQQPWQISNHMVIQSQCKALKTYQVNLVWQVIGNSTIMVVIAVLDMIIYSDTTHDF</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-05-03" modified="2025-06-18" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3Q8</accession>
    <name>MAP2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methionine aminopeptidase 2</fullName>
            <shortName evidence="1">MAP 2</shortName>
            <shortName evidence="1">MetAP 2</shortName>
            <ecNumber evidence="1">3.4.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Peptidase M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ORF">PGTG_16374</name>
    </gene>
    <organism>
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>GENOME REANNOTATION</scope>
        <source>
            <strain>CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="1">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="1"/>
    <dbReference type="EMBL" id="DS178343">
        <property type="protein sequence ID" value="EFP91183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335602.1">
        <property type="nucleotide sequence ID" value="XM_003335554.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L3Q8"/>
    <dbReference type="SMR" id="E3L3Q8"/>
    <dbReference type="FunCoup" id="E3L3Q8">
        <property type="interactions" value="772"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L3Q8"/>
    <dbReference type="EnsemblFungi" id="EFP91183">
        <property type="protein sequence ID" value="EFP91183"/>
        <property type="gene ID" value="PGTG_16374"/>
    </dbReference>
    <dbReference type="GeneID" id="10528845"/>
    <dbReference type="KEGG" id="pgr:PGTG_16374"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16374"/>
    <dbReference type="eggNOG" id="KOG2775">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015857_7_1_1"/>
    <dbReference type="InParanoid" id="E3L3Q8"/>
    <dbReference type="OMA" id="PFAKRWL"/>
    <dbReference type="OrthoDB" id="7848262at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004239">
        <property type="term" value="F:initiator methionyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008235">
        <property type="term" value="F:metalloexopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01088">
        <property type="entry name" value="MetAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03175">
        <property type="entry name" value="MetAP_2_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050247">
        <property type="entry name" value="Met_Aminopeptidase_Type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002468">
        <property type="entry name" value="Pept_M24A_MAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00501">
        <property type="entry name" value="met_pdase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777:SF2">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0031">Aminopeptidase</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0645">Protease</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000407666" description="Methionine aminopeptidase 2">
        <location>
            <begin position="1"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="31"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="66"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="209"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="343"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="437"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="437"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <sequence length="456" mass="49884" checksum="3E186ACE45D67CED" modified="2011-01-11" version="1">MTIPVPKPAHPAAPDTNTDGLPNPKVVVPVEADEEEDDDDEEGKEDADGAVPAASHFSTHALDGTKKKKKKKKKPKKKKTGEAGAGGQSEPPRVPVSKLFPNGNYPSGEESAYLGENSYRTTSSEKRELERLAAQEDPESAENYNSIRRAAEVHRQVRRYVQQTVKPGMSMTEIAEMVEDGTRALVEVDGLQRGIGFPTGVSLNHCAAHYTPNAGDTIVLSADDVLKVDFGVQIGGRIVDSAFTMTFNNKYDKLLEAVRAATNTGIREAGIDARLSDIGASIQETMESYEVEVDGKVHKVKSIRNLTGHNILPYHIHGGKSVPIVANSDESAIMEEGDHFAVETFGSTGRGYVMDDGECSHYAKNPDVNKPIRLARAKTLLNTINKHFDTLPFCKRYLDRLGESRYYAALDNLVNLGIVQAYPPLSDIQGCMTAQYEHTIILRPTCKEVVSRGDDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3R9</accession>
    <name>E3L3R9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_16385</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178343">
        <property type="protein sequence ID" value="EFP91194.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335613.2">
        <property type="nucleotide sequence ID" value="XM_003335565.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L3R9"/>
    <dbReference type="GeneID" id="10528856"/>
    <dbReference type="KEGG" id="pgr:PGTG_16385"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16385"/>
    <dbReference type="HOGENOM" id="CLU_148184_0_0_1"/>
    <dbReference type="InParanoid" id="E3L3R9"/>
    <dbReference type="OrthoDB" id="10383255at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP91194.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="108" mass="12146" checksum="CCC960F28C785137" modified="2012-04-18" version="2">MSAGNVYSLDDFSMYLMPKRVAPVCPPCQFDILPSVFEDDDDDEIEIEQEMTVLSKANKVFRKMIVRAKHPKQSSPKFFSASRLRSITLSRRSSKSPVISVSPSNSSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3X6</accession>
    <name>E3L3X6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17108</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178344">
        <property type="protein sequence ID" value="EFP91251.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335670.2">
        <property type="nucleotide sequence ID" value="XM_003335622.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L3X6"/>
    <dbReference type="GeneID" id="10528996"/>
    <dbReference type="KEGG" id="pgr:PGTG_17108"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17108"/>
    <dbReference type="HOGENOM" id="CLU_174214_0_0_1"/>
    <dbReference type="InParanoid" id="E3L3X6"/>
    <dbReference type="OrthoDB" id="2513946at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91251.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="80" mass="7768" checksum="CACD213FD728ED18" modified="2012-04-18" version="2">MEGTGRGVGKPAQGGKVFVPPRTSTVRSSEIRGSKDDSAASDFSSVVVDPPTNTGNSGNQSGGRGGSGSVGEGASGRGDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3Z2</accession>
    <name>E3L3Z2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_17124</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178344">
        <property type="protein sequence ID" value="EFP91267.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335686.2">
        <property type="nucleotide sequence ID" value="XM_003335638.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L3Z2"/>
    <dbReference type="GeneID" id="10529013"/>
    <dbReference type="KEGG" id="pgr:PGTG_17124"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17124"/>
    <dbReference type="HOGENOM" id="CLU_437501_0_0_1"/>
    <dbReference type="InParanoid" id="E3L3Z2"/>
    <dbReference type="OrthoDB" id="2507691at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172439" evidence="2">
        <location>
            <begin position="23"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="241"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="269"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP91267.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="625" mass="71493" checksum="29E009DAA8D08A1D" modified="2012-04-18" version="2">MKLGVQFYPIFILEWAALPITASLPLAKDINMAAPSHPKYRLPDLNESLQDDEQLTDQASCFLDTRPSPVQIPTTHLGSEHGQASSQFSASTIQAEAINMSIRTFQAGFLEHRPPSTPVPEGTQAGSKEKAVLSEPWKYNPAAEAPSMKRLKRLDGKQTTGSREEPKKALRMLLPKPDFGAKEAPVFSRFPATIQTYPIPSTSSNFFQSAPLKRKKNLNPAVDQAQHSSFIQEKFEGNISGSEAKRLQQPRILPEKKKVYHPRKIAPEATSSKSKRLQSNVDGQTKRKRGRPKKQASALDDSDQIAVEKQKRIRDDDELFDLHDWNFIRETGQSELEKNEAVHINPQTGFPKGSQPNRVLQTLRDAISFQFKPGEFYIPLNHENQFFSKWRTWIRNEFVFPKRYSDHFIRKGLFWTMYDKINSQVKFDGYPIYSNGIIGLVSSNIERFSQTSRNPLPRERIEAIINIVGESTKAATLLILVYLSIFREIKGGIVRSDTIEEILTTLAEVWRSVGDENPWKFIQEFEWASGLLELLSTGKTYDNSTYKGSSAKVMNYRVAWDLVEYWTKRTTRVGKDGVTKKKRYFSRHVMELINKMVFYLNHDTIIKRVEAYKQRREKKLASGLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3L5Y3</accession>
    <name>E3L5Y3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">GH16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_18409</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SKN1/KRE6 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178355">
        <property type="protein sequence ID" value="EFP91958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336377.1">
        <property type="nucleotide sequence ID" value="XM_003336329.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L5Y3"/>
    <dbReference type="FunCoup" id="E3L5Y3">
        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="GeneID" id="10531910"/>
    <dbReference type="KEGG" id="pgr:PGTG_18409"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18409"/>
    <dbReference type="eggNOG" id="ENOG502QR13">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010811_3_0_1"/>
    <dbReference type="InParanoid" id="E3L5Y3"/>
    <dbReference type="OMA" id="TAPFDDY"/>
    <dbReference type="OrthoDB" id="412647at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015926">
        <property type="term" value="F:glucosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006078">
        <property type="term" value="P:(1-&gt;6)-beta-D-glucan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031505">
        <property type="term" value="P:fungal-type cell wall organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02180">
        <property type="entry name" value="GH16_fungal_KRE6_glucanase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000140">
        <property type="entry name" value="Beta-glucan synthesis-associated protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.200:FF:000135">
        <property type="entry name" value="Related to KRE6-glucan synthase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.200">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000757">
        <property type="entry name" value="Beta-glucanase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005629">
        <property type="entry name" value="Skn1/Kre6/Sbg1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31361">
        <property type="entry name" value="BETA-GLUCAN SYNTHESIS-ASSOCIATED PROTEIN KRE6-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31361:SF15">
        <property type="entry name" value="GH16 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03935">
        <property type="entry name" value="SKN1_KRE6_Sbg1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51762">
        <property type="entry name" value="GH16_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="144"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="domain" description="GH16" evidence="11">
        <location>
            <begin position="214"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="69"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP91958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="623" mass="67256" checksum="4C51DFB6298571C9" modified="2011-01-11" version="1">MPSDDIHLTSKASHIDEASESSFAGELSDEKANWAPADNRASVMNAIYSDQNYLSSASYLLGANQRPDSAASSYPSFRGSQIDPGKASRRSYMEDYQDFDAELEDELHNDDGDDDEDNLTGSPKKSKLACGNLFPMRGILNMGAVMLLALALVTIFGILPILTYYGAHRATKQLAVNSGGFNLGGINASGQVPLIHNLAGVIDPATPQSVMSRKGFDGTQYNLVFSDEFNTDGRTFWPGDDPYWEAVDLHYWATGNLEWFDPDAITTNNGNLNITITKELIHDLNYRSGMLQSWNKFCFTGGYIEVSISLPGSPRISGFWPGAWTMGNLGRAGYGATTDGTWPYSYNSCDLGTLKNQTNVAGTGPAAALRAPGGGPLSYLPGQKLSSCTCPGGEHPGPNVKTGRGSPEIDIIEAQVTSPGTGGNIGQASQSVQFAPFDDGYNWQEDGARVYNPRVSNINAYKGGIYQEAVSVVSTTDQTAYEATGGNFQIFGFEYTTGPKGSITWSAGDTATWTMSSSAVGPNPNVQISQRVVSEEPMYIILNLAISEAFETVDAAHLPTPARMLVDYVRVYQRKGQENIGCSPKNFPTESYINKHINAYTNPNLTTWDQAGYTFPKNSLTGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3L639</accession>
    <name>E3L639_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_17845</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178356">
        <property type="protein sequence ID" value="EFP92014.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336433.2">
        <property type="nucleotide sequence ID" value="XM_003336385.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L639"/>
    <dbReference type="GeneID" id="10531967"/>
    <dbReference type="KEGG" id="pgr:PGTG_17845"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17845"/>
    <dbReference type="HOGENOM" id="CLU_398564_0_0_1"/>
    <dbReference type="InParanoid" id="E3L639"/>
    <dbReference type="OrthoDB" id="2497097at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="79"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="214"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="422"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="79"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="217"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP92014.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="710" mass="81135" checksum="AAB6AECD9D817BDC" modified="2012-04-18" version="2">MFSPCRFCETSRTSRPSRSKMRFPYGSITPHGHPLYFAFWSLATVSAANLDDLDVNRWDQGPSARKLFTSSPDYPISSWAPSSHESNVQSEVSNSGAHRLSPSPLTEQNLLERLGDSSVIGEPSKDYFELLADDQAGSRKRVKHSQGQQLSTRRTPIYYEFMPGSSHAHDGDAAHLRSSPEGQVNQGQIQAQVQRVVPLDLLNIPMEHLTSQVAQSVEERAPSTHEMSPKRKISESVETHTTNEEASQNVLPPGLFGAGGQILVPIVYQYYHAFAQKLDSSDFKRAKVSPQNIHHTLPIGFMIRPGCRITRILDDSHQRTQNWRLLKKHYKGLLLTIHNFHLRHPGSPAEHMVHVRSIFNRVDKEIFEPENGVPFLGISQFTAKGWKEKFSDTVFEDVQKSLLKYMSSGSTELLPRLASDLIKSYHRDLESNESTLRNHREQLSKLEASSEIKMQPYFRARFDYIFSLPDGYPRFQAFLDRGEGALFPGHPSYEVFNKVSEKFEQEFRTKIHRTNLRTDHAALQIAVLSEKTSKDVQVMRVLNSAMDRPYTIEPFMSVYNHLIKSVEFLHVAILGQLELTGGERNRRRLDVLSWISEEITKPKESLPVFGMIQDNIDSSNMEDRMLSGEKLFGPIQVSLIKYFSSSELARSGHFPETRFTAAFALASWYEVNHPEEFNNFFFHQSSRCENLEEALELKKPYIHPFYCRDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3L7J5</accession>
    <accession>E3L5A5</accession>
    <name>E3L7J5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">CDP-diacylglycerol-serine O-phosphatidyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_17467</name>
        <name evidence="13" type="ORF">PGTG_18518</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <reference evidence="13" key="3">
        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178351">
        <property type="protein sequence ID" value="EFP91730.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="DS178366">
        <property type="protein sequence ID" value="EFP92520.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336149.2">
        <property type="nucleotide sequence ID" value="XM_003336101.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336939.2">
        <property type="nucleotide sequence ID" value="XM_003336891.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L7J5"/>
    <dbReference type="FunCoup" id="E3L7J5">
        <property type="interactions" value="74"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L7J5"/>
    <dbReference type="GeneID" id="10531670"/>
    <dbReference type="GeneID" id="10538517"/>
    <dbReference type="KEGG" id="pgr:PGTG_17467"/>
    <dbReference type="KEGG" id="pgr:PGTG_18518"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17467"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18518"/>
    <dbReference type="eggNOG" id="ENOG502QPJG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049944_5_0_1"/>
    <dbReference type="InParanoid" id="E3L7J5"/>
    <dbReference type="OrthoDB" id="448573at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050324">
        <property type="entry name" value="CDP-alcohol_PTase-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269:SF61">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--SERINE O-PHOSPHATIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="21"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP91730.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP92520.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="314" mass="34399" checksum="393A671271FB7DFD" modified="2012-04-18" version="2">MPGTNPEMTIPASAPGETAPLSANTTPVDPPSTVNPVIMDNSRRESIANRNQDPVTVALKQFVNDDRHFSLLRNFRLADLITIFNGVCGSLSIFSSAYFLITLDLEHLKRACLLPIFSLGFDFLDGKVARFRGESSVLGQELDSLADLISFGVAPALVGFTIGLRHPTDVLILTFFVCAGLTRLARFNSTAAFNQRIRAIDEPPQKTGAKNASNSFEGLPIPTSLSLVAAMYESIRRAQIQLPLIYQHLLSDHNSALSINPENSLWLKNVHLNGNQLLGGLVKNSFGIQVHWFSFAFAGWGFLMLSKTFKVPKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3L7Z7</accession>
    <name>E3L7Z7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18187</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178370">
        <property type="protein sequence ID" value="EFP92672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337091.1">
        <property type="nucleotide sequence ID" value="XM_003337043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L7Z7"/>
    <dbReference type="GeneID" id="10539001"/>
    <dbReference type="KEGG" id="pgr:PGTG_18187"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18187"/>
    <dbReference type="HOGENOM" id="CLU_2027875_0_0_1"/>
    <dbReference type="InParanoid" id="E3L7Z7"/>
    <dbReference type="OrthoDB" id="2508173at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="114" mass="12746" checksum="4CDDC86C89DFE587" modified="2011-01-11" version="1">MIWTGQGQQRLLSGSILIMQFYVLMMGPISTCEAHVLRSTENHPMKRDVADDPSQASSHLQFPSFKITNIIHRRSPLKGRVRSPCESSERVISIGNGRTSCRPKHDCNGPNCPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3L845</accession>
    <name>E3L845_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_18739</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178371">
        <property type="protein sequence ID" value="EFP92720.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337139.2">
        <property type="nucleotide sequence ID" value="XM_003337091.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L845"/>
    <dbReference type="GeneID" id="10539216"/>
    <dbReference type="KEGG" id="pgr:PGTG_18739"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18739"/>
    <dbReference type="HOGENOM" id="CLU_026798_0_0_1"/>
    <dbReference type="InParanoid" id="E3L845"/>
    <dbReference type="OrthoDB" id="2499742at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="367"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="3">
        <location>
            <begin position="116"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP92720.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="389" mass="44711" checksum="21EB0BC546253F5A" modified="2012-04-18" version="2">MAHNKRKRSQHKNKLLRERRERASLVESAIGPSGRKFYWQNVKYEDLNIYPTIPTALVRGKLQYTRPPTTEEIAMASDTVQTFRLLKTGCNIVRDPQDPKSIIAIIEFTKFSDLTQADREELNFVSTFLRKTTKFISYVKSKQRAWGGKMWGIGWRKSSDEDQIAGRYIKAFEAVNAQAYHDLFSLSGRVGEIVGRNFKKLAEIPFGSNRELMAEHGLPSLAALEYGEELTESDCAPHLTFTTNGFFNPPHTDDEDVSKYAFVMFLPTHTKDGSLATDEDSYDITPGPFIFPDHKFGINFDHQFGVVKMIWQANKYKHCTMPSSQSSRFTRLGMSVQINLGLKNACVKYQEGRYNSVANYFGDHFFYWYRSCLQNMLVVCAFLFTFLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8D7</accession>
    <name>E3L8D7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7729 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_18591</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178373">
        <property type="protein sequence ID" value="EFP92812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337231.1">
        <property type="nucleotide sequence ID" value="XM_003337183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L8D7"/>
    <dbReference type="GeneID" id="10542525"/>
    <dbReference type="KEGG" id="pgr:PGTG_18591"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18591"/>
    <dbReference type="HOGENOM" id="CLU_080223_1_0_1"/>
    <dbReference type="InParanoid" id="E3L8D7"/>
    <dbReference type="OMA" id="NATCVEC"/>
    <dbReference type="OrthoDB" id="2536450at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056146">
        <property type="entry name" value="DUF7729"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34862">
        <property type="entry name" value="SPARK DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34862:SF1">
        <property type="entry name" value="SPARK DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24855">
        <property type="entry name" value="DUF7729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174098" description="DUF7729 domain-containing protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7729" evidence="2">
        <location>
            <begin position="55"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF24855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP92812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="266" mass="28608" checksum="A5516376D59D610C" modified="2011-01-11" version="1">MAFSHQLISLLVCLAGYQTVIAHPWNVGVGQPQEDQPRVGFRDSLAKKSKLSASCQLAAAGLLASEFGTCANILELVSIFDAKESLISPMNSWITGACSATPCKQEGLAAASHMIRTGCESDLKEGSIAAVAIYSVITHYNATREMFCTQYKENLNYCLPSVLGNVEDQSGENITTDEVMSLIAGKFTVADQSFISVPKETYCNACGQAIVSRSAMMIDAIRQDPVGIEFNYTAGHTVHRISDICGASFENQELPDTVQVALPQPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3L9F6</accession>
    <name>E3L9F6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19141</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178384">
        <property type="protein sequence ID" value="EFP93181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337600.1">
        <property type="nucleotide sequence ID" value="XM_003337552.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L9F6"/>
    <dbReference type="GeneID" id="10543331"/>
    <dbReference type="KEGG" id="pgr:PGTG_19141"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19141"/>
    <dbReference type="HOGENOM" id="CLU_2005012_0_0_1"/>
    <dbReference type="InParanoid" id="E3L9F6"/>
    <dbReference type="OrthoDB" id="2495228at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174118" description="Secreted protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="124" mass="13976" checksum="2C8385E988C598A6" modified="2011-01-11" version="1">MRFCSLITLVALLLIQSEAAHSKFICNDGSKKDAKHGICVRKISQQHDSTNKDLPPLKDMDVLVIDASPMGNGFTCAKLEIAKLPVEARYCCDFPPTHKTLPHTKKYIDDHCYTRDGRRKHPRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3LAD2</accession>
    <name>E3LAD2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18921</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178396">
        <property type="protein sequence ID" value="EFP93507.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337926.2">
        <property type="nucleotide sequence ID" value="XM_003337878.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LAD2"/>
    <dbReference type="GeneID" id="10543652"/>
    <dbReference type="KEGG" id="pgr:PGTG_18921"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18921"/>
    <dbReference type="HOGENOM" id="CLU_1504155_0_0_1"/>
    <dbReference type="InParanoid" id="E3LAD2"/>
    <dbReference type="OrthoDB" id="10405880at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="120"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93507.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="179" mass="17766" checksum="6DA02AE00AC34BA0" modified="2012-04-18" version="2">MDKLKLKPNSTGAPGAGSGTPDQNPKSRTEAGGGSGTPPAGPGKTSKPNNGMTRRAEPASKSAEENKASFMEKLKAESSASGRPAGTPTAELTKQADAPGGGSDNKKAAFLEKLKAKKASGGGLSKGGDGGAGAMGAKSPMSKRADSPSGAPEEKKGGGLLDKLKSKLSAADHSFIYNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3LAV1</accession>
    <name>E3LAV1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19337</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178403">
        <property type="protein sequence ID" value="EFP93676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338095.1">
        <property type="nucleotide sequence ID" value="XM_003338047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LAV1"/>
    <dbReference type="GeneID" id="10534965"/>
    <dbReference type="KEGG" id="pgr:PGTG_19337"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19337"/>
    <dbReference type="HOGENOM" id="CLU_106390_0_0_1"/>
    <dbReference type="InParanoid" id="E3LAV1"/>
    <dbReference type="OrthoDB" id="2507791at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="11"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="227" mass="24602" checksum="505EB9DBC5F4706F" modified="2011-01-11" version="1">MDLPYSGSDTSESHTDSGDTVPHRCDGTPPATTTILAQNFQIGSETTAPIHHPPSFQEFAHKANKATSSAPLVPSEPVLDTEIQQISADEFNRNMKAAEQATRGLRNLKLPRSLKEKVKSLEKSLVSTKRTWEETGEIPDDEAIKIHQGPTSAHSSSTKTFTKKTPRKKVKRDPPTKPVDLLNKELPHPPANPLPSTTSNRPMDQTVATSQSTPSIISGAPCEPGSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3LB98</accession>
    <name>E3LB98_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_19858</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178411">
        <property type="protein sequence ID" value="EFP93823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003338194.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LB98"/>
    <dbReference type="GeneID" id="10535274"/>
    <dbReference type="KEGG" id="pgr:PGTG_19858"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19858"/>
    <dbReference type="HOGENOM" id="CLU_1511319_0_0_1"/>
    <dbReference type="InParanoid" id="E3LB98"/>
    <dbReference type="OrthoDB" id="10557916at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP93823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="178" mass="19518" checksum="007B879088840C47" modified="2011-01-11" version="1">MVKTKKQTARQQFGHIGHRNVLSVELDEPLVVEPVDGVVQETIVALSDNVAGLSAEIALLVDLLCDADDSTIQRLRWLPPGLVTHTHFGPHTEIPGSERVAVLVLLGGVRHTPWGGFLEKPPSKRMDSVPSARNLVAKHPTDARAGLASFGRFRKRPRDAWQARPSRGWCTARCPSDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3NY50</accession>
    <name>E3NY50_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_20455</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS990031">
        <property type="protein sequence ID" value="EFP94499.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338918.2">
        <property type="nucleotide sequence ID" value="XM_003338870.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NY50"/>
    <dbReference type="GeneID" id="10527794"/>
    <dbReference type="KEGG" id="pgr:PGTG_20455"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20455"/>
    <dbReference type="HOGENOM" id="CLU_033090_2_1_1"/>
    <dbReference type="InParanoid" id="E3NY50"/>
    <dbReference type="OrthoDB" id="2513067at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP94499.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="412" mass="47617" checksum="A9D10494D59B11B6" modified="2012-04-18" version="2" fragment="single">PVLEALEVLTHAYDRGLWSSYRLNAGEENGELIKDNLDEKNKLMHELQSSLLPSIKHHLETLDPPHDFNDYPSLEIKTTLETLSNLDQTMQKTLHYLNSSPLKVFEPSNAYDHHFKQCKTFRCHALLEPINLLEINLRSLFLAYIKLIESRNLPIKHPEDPRYRAPTPREETVKCLKSIDQAIRWSQASDFALLQLKWRGVAESFTNSMTRLARLIDLISRHARRNTILRKHIVEQARLIVPVIKLLRTLYNKISNTTKKKQPFTLDPEVNSDTLYTLELDSSIIFHACNVHVKNLEIRHMYGSMVDTSEALRKRNKSLSRTVESTLFLLALYIIPLSPKVDHSGIPLKVTSRPGFPSGKFYGGPRRSVLWMLCMSMKRRIDNQRKNALNVYFFRVVFQLPLTALEMAVIVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3NYD1</accession>
    <name>E3NYD1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrotransposon Copia-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_20536</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS990085">
        <property type="protein sequence ID" value="EFP94580.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338996.2">
        <property type="nucleotide sequence ID" value="XM_003338948.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NYD1"/>
    <dbReference type="GeneID" id="10527956"/>
    <dbReference type="KEGG" id="pgr:PGTG_20536"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20536"/>
    <dbReference type="HOGENOM" id="CLU_054434_2_0_1"/>
    <dbReference type="InParanoid" id="E3NYD1"/>
    <dbReference type="OrthoDB" id="2504811at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP94580.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="14420" checksum="494404EA94E48843" modified="2012-04-18" version="2">MPYRTTQNPSPLIPLVPQLDGNLVTFTSWRSRLKDVLVIQGVLDIVQGKIPRPPAESPDSKPIVHTKRGWNPEDFCLDWDHLLDMAHSTIKLTLLVGLSIRYRDLKPASKLYNVICEAYEKNTWAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3NYF8</accession>
    <name>E3NYF8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20563</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS990112">
        <property type="protein sequence ID" value="EFP94607.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003339023.2">
        <property type="nucleotide sequence ID" value="XM_003338975.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NYF8"/>
    <dbReference type="GeneID" id="10528067"/>
    <dbReference type="KEGG" id="pgr:PGTG_20563"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20563"/>
    <dbReference type="HOGENOM" id="CLU_097644_0_0_1"/>
    <dbReference type="InParanoid" id="E3NYF8"/>
    <dbReference type="OrthoDB" id="2505769at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="1">
        <location>
            <begin position="10"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP94607.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="249" mass="28776" checksum="FB7B77ED839FD598" modified="2012-04-18" version="2">MAKYRPCNKRNANRLNGEMHLIGFRPGNDRGKSAGTYAQRTDLTPAQSAHDDTLWAKLQSFNKFLSQRMEAISHSAYQQNKELMDKRGIPNWSQEEWAEMRKEDQIEFQFASNVSVTFNDFYNKPHQDKKDINGWTYGIFAYVDLETGKPIPPPSTELGHGFLFPRHAYLIDFVKSSGIMELVWQTTKFEHCTTMPPPSLRKQKGRTWTHQGCSFQINKYLAKVAQELKDASPQFVEKKTLCKKQKYGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>H6QP85</accession>
    <name>H6QP85_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_20778</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EHS63199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890754.1">
        <property type="nucleotide sequence ID" value="XM_003890705.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QP85"/>
    <dbReference type="GeneID" id="13541382"/>
    <dbReference type="KEGG" id="pgr:PGTG_20778"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20778"/>
    <dbReference type="HOGENOM" id="CLU_2528517_0_0_1"/>
    <dbReference type="InParanoid" id="H6QP85"/>
    <dbReference type="OrthoDB" id="10295714at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="84" mass="9025" checksum="32742E1625B0C326" modified="2012-04-18" version="1">MKGRQLSAPAQVNDDDYSNLFTQGWSSLVPGISSCRTTFEQNAPVKAAIQAAASLASKIQAITFQYGQCVCNNKILGDETIDRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQ34</accession>
    <name>H6QQ34_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">DNA repair protein rad4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_21013</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the XPC family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EHS64662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890368.1">
        <property type="nucleotide sequence ID" value="XM_003890319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQ34"/>
    <dbReference type="FunCoup" id="H6QQ34">
        <property type="interactions" value="85"/>
    </dbReference>
    <dbReference type="STRING" id="418459.H6QQ34"/>
    <dbReference type="GeneID" id="13542749"/>
    <dbReference type="KEGG" id="pgr:PGTG_21013"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21013"/>
    <dbReference type="eggNOG" id="KOG2179">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003639_2_0_1"/>
    <dbReference type="InParanoid" id="H6QQ34"/>
    <dbReference type="OrthoDB" id="300780at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000111">
        <property type="term" value="C:nucleotide-excision repair factor 2 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071942">
        <property type="term" value="C:XPC complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2460:FF:000001">
        <property type="entry name" value="DNA repair protein Rad4 family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.20.110:FF:000005">
        <property type="entry name" value="Unplaced genomic scaffold supercont1.2, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.20.110">
        <property type="entry name" value="Rad4, beta-hairpin domain BHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2460">
        <property type="entry name" value="Rad4, beta-hairpin domain BHD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.260.10">
        <property type="entry name" value="Transglutaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018327">
        <property type="entry name" value="BHD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004583">
        <property type="entry name" value="DNA_repair_Rad4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018325">
        <property type="entry name" value="Rad4/PNGase_transGLS-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018326">
        <property type="entry name" value="Rad4_beta-hairpin_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018328">
        <property type="entry name" value="Rad4_beta-hairpin_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042488">
        <property type="entry name" value="Rad4_BHD3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036985">
        <property type="entry name" value="Transglutaminase-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12135:SF0">
        <property type="entry name" value="DNA REPAIR PROTEIN COMPLEMENTING XP-C CELLS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12135">
        <property type="entry name" value="DNA REPAIR PROTEIN XP-C / RAD4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10403">
        <property type="entry name" value="BHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10404">
        <property type="entry name" value="BHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10405">
        <property type="entry name" value="BHD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03835">
        <property type="entry name" value="Rad4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01030">
        <property type="entry name" value="BHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01031">
        <property type="entry name" value="BHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01032">
        <property type="entry name" value="BHD_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rad4 beta-hairpin" evidence="7">
        <location>
            <begin position="344"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="domain" description="Rad4 beta-hairpin" evidence="8">
        <location>
            <begin position="396"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="domain" description="Rad4 beta-hairpin" evidence="9">
        <location>
            <begin position="466"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="94"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="580"/>
            <end position="618"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="640"/>
            <end position="665"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="680"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="102"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="143"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="185"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="583"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="644"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="711"/>
            <end position="728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="743"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="774"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="841"/>
            <end position="850"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS64662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="860" mass="96350" checksum="22B063E6BAA3C724" modified="2012-04-18" version="1">MISRSWSDAEVLYQNSDDVTGKGKAKGNHPEDQFLQLLESGEPIHGVKSLMKRALLMKGSRDMSAQLFTSCLRALDIPARLVFSLQPVTWRGAGAGGKSANPAATTQEETVTGGSKSENRINSGVNPTKPKSRTAQNAKSKSKRGEYATKAEKAAKAIKAKSVRIDNQTQSLRGTDEGEDEEGDLNSQVETVPVASSSRHSPVIKLRKSRPPTKSRNWAKSPSPEPQEMNRTPVFWTEVYSRPMKEWYCVDVTRKKMRCKNIMEPSRNNPENKMIYVIAYEEDNFIRDVTARYAHSFGATTMKARLPSKKNEEDWFERAAAILKRPYKLGRDIKEDTEIEKARVTEAMPTTVTGFKNHPKYALERHLRREEVIYPKRPIGTFRGDSVYPRSSVIVCKSTETYMREGKRVKGGENPLKMVKPRAVTINRKRETELLKMDGQPVPLQGLFAEWQTELLIPPPIVDGVIPRNGYGNFDLFAPHMLPQGAKHLPYKGIAKTAKKLKVSYADAVVSFEFHRSRAMPLIDGIIVPELDAEFVLDAYWTSEEAQEVKEFEKLEERCLKRWKKLIIGLRIRQRLQREYGPQATQISDSQQLTKEPSNSRKTNSQQTSTSANQPSIVALTPDSKLPFELYTDHQMNPMIVPQADHDGDHIPSKDLRHSEPADLEEPTVADVFCPRSGKAVESETLPCSPSLTKECEDSPEIDTPRKTRQSTRVSRTAQPIKLANSSKGIKRKSASEHALGLTKQTPSKTGSRSRQTRRNGSIHSIDQDPYDSSPLTSPPSASEHSDYSAILSPPKRAKVNQKSPKELSVIPRESSPEDTPDKNPRASTRPTRRAAVKANENISLTSAAQGSKRILRSRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQC4</accession>
    <name>H6QQC4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EHS62535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890248.1">
        <property type="nucleotide sequence ID" value="XM_003890199.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQC4"/>
    <dbReference type="GeneID" id="13542216"/>
    <dbReference type="KEGG" id="pgr:PGTG_21084"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21084"/>
    <dbReference type="eggNOG" id="KOG3544">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041946_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQC4"/>
    <dbReference type="OMA" id="MPARISV"/>
    <dbReference type="OrthoDB" id="2503759at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003605725" evidence="1">
        <location>
            <begin position="23"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="407" mass="40737" checksum="56368620CDFE63A1" modified="2012-04-18" version="1">MRVSCFFVSVAISMMLAEQNNALPLGGLSSLPLLGNQGQQVGQGLPLLNTVGSLANGLQSLPLLGSVGSVAPVTQGLTSRVGSLNNAVPQVNKVVLRRQSLPLLNTVGSIANAVEGLPLLGSLGSIAPVTQGVTSQVGPLNNDLPNVKKVVSGTAQALPATQLSSSKISLQRRQGLPLLNTLGSITNGVQSLPLLGSVGSVAPVTQGLTSRVGSLNNSVPQVNKVVSGTTSTLPVSQLTSKLPLLHKRANSVPVVGTLPVVGSIPGVKELGAIPGFSSLPSMDSLPGYSGLQAFDAIPGLSSVEAIGDVIPLSGFTKRSGGIPGLGSIPVLSSLPGVQSIPGMSSIPGLGAIPSADSIPGFSSLPFNNGIPGLSGVKGLTSALPIPNIFKRDSGLSSLPLLGALPRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>H6QR77</accession>
    <name>H6QR77_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21399</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EHS63059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889989.1">
        <property type="nucleotide sequence ID" value="XM_003889940.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QR77"/>
    <dbReference type="GeneID" id="13540488"/>
    <dbReference type="KEGG" id="pgr:PGTG_21399"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21399"/>
    <dbReference type="HOGENOM" id="CLU_1074158_0_0_1"/>
    <dbReference type="InParanoid" id="H6QR77"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="259" mass="28872" checksum="28E1E2962339071C" modified="2012-04-18" version="1">MNSRHQYLHPAPLATAHEVDHGRIAGRMYFATSSTSYFAYSNSPICPSHGKRTAVLTAGRTASRSSWIGLADRGTIPNGAPLPARPPWPRKSTWRCGGLAYTIDPPTGYYDAWEYAKQTPSQLQERYPKGYLSFEVPLGYTNMICRDWPRLVEIFGPPENRTRAEVVQPPMTTPITTWSAASSPQVLEFVQRVRADYDAMIAQTGSSVSWATYFKNNTTCRADFPMIAGFSNALIIRRYQSLVRTTPRPPMLGSSNTIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRL3</accession>
    <name>H6QRL3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21499</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EHS63283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889856.1">
        <property type="nucleotide sequence ID" value="XM_003889807.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRL3"/>
    <dbReference type="GeneID" id="13540704"/>
    <dbReference type="KEGG" id="pgr:PGTG_21499"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21499"/>
    <dbReference type="HOGENOM" id="CLU_060582_0_0_1"/>
    <dbReference type="InParanoid" id="H6QRL3"/>
    <dbReference type="OrthoDB" id="2507532at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="272"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="280"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="292"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="385" mass="42440" checksum="68A4035B9FB0C541" modified="2012-04-18" version="1">MLVKHFNSRITWELTQAGHIRSFLAIGQNSLHVAANKLSPGSFMPGAFASCDLFIDLLIDPTTRKATTLKHPYPPRELFTVTEVRTATCQEQPLTAQPHPRGSRDIAVIRIQKDMFEAETVDTSSIQSLMTAWTTDGITSVSGLCCKTCQSVKKADPHNIIEKSRLSVDNGGPPQHLYMLVHAALIIDESAQQLFMASLDFPFQLDFHGVGYTLFSRGFWNGTHYWTKMLKNIGGISGIWMHDDRANGGIARLEKFVAESIANIRRDNKDAQGLVPRYADNGRADNGRDANNRSNLESAPGQTPKSISGDLKRKPSGEFKVRLKIMKPPETSPAAKIEDKEAPVPPTSKEPMPPKKKKDGIPKSKVVKPFRAGSRASTRKAALQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRY0</accession>
    <name>H6QRY0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21595</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EHS63464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889749.1">
        <property type="nucleotide sequence ID" value="XM_003889700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRY0"/>
    <dbReference type="GeneID" id="13543126"/>
    <dbReference type="KEGG" id="pgr:PGTG_21595"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21595"/>
    <dbReference type="HOGENOM" id="CLU_3033443_0_0_1"/>
    <dbReference type="InParanoid" id="H6QRY0"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="55" mass="5740" checksum="8581829C9BFAFE1A" modified="2012-04-18" version="1">MLASGLYTSLLTAVNCRSSSNRSVVLTVNGTPRVAIPNSIPRLESTSDLKYGGNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>H6QS26</accession>
    <name>H6QS26_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21602</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EHS63472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889683.1">
        <property type="nucleotide sequence ID" value="XM_003889634.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QS26"/>
    <dbReference type="GeneID" id="13542834"/>
    <dbReference type="KEGG" id="pgr:PGTG_21602"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21602"/>
    <dbReference type="HOGENOM" id="CLU_2387247_0_0_1"/>
    <dbReference type="InParanoid" id="H6QS26"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="94" mass="9860" checksum="DDC12C11BAE00167" modified="2012-04-18" version="1">MAIQPWLDTVPRLSAIGPLGIQIVHHGVSIGATLINLKGVDPKLEPAELPSGVSLEKALLTSVLTLLSYPTACVPGPQTNVSYILFTFIDGVPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSF1</accession>
    <name>H6QSF1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21767</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EHS63681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889569.1">
        <property type="nucleotide sequence ID" value="XM_003889520.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSF1"/>
    <dbReference type="GeneID" id="13542712"/>
    <dbReference type="KEGG" id="pgr:PGTG_21767"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21767"/>
    <dbReference type="HOGENOM" id="CLU_2623132_0_0_1"/>
    <dbReference type="InParanoid" id="H6QSF1"/>
    <dbReference type="OrthoDB" id="10272541at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="78" mass="8874" checksum="B08CCC7B9D55F498" modified="2012-04-18" version="1">MLGHGIRGLTTRETEANLGRTVRWNSAVRLRQQGEEKLEKSDVSFLTFGFQTKMLGHGIRGLTTRETEANLKENQDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSW3</accession>
    <name>H6QSW3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21890</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178300">
        <property type="protein sequence ID" value="EHS63852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889413.1">
        <property type="nucleotide sequence ID" value="XM_003889364.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSW3"/>
    <dbReference type="GeneID" id="13540618"/>
    <dbReference type="KEGG" id="pgr:PGTG_21890"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21890"/>
    <dbReference type="eggNOG" id="ENOG502S1WT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003292_8_2_1"/>
    <dbReference type="InParanoid" id="H6QSW3"/>
    <dbReference type="OrthoDB" id="2506773at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052055">
        <property type="entry name" value="Hepadnavirus_pol/RT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050:SF7">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="7"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="32"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="628" mass="72234" checksum="793C2A21C59C4908" modified="2012-04-18" version="1">MNQGSFHNNSPNNYQYNSNYDNNHSHAHQHHASFVNNYDNNNGGNHHQPMGDGRKRFRSSGNKSETNDKLSSSMTGGSKPPFCATKATWPVTVSCEMNIEEWKQALDRAGLLPEMLHILEGFKIGFDQGIPDHKVGNERWFTPDNHSSAVNAEEKIQKSIKEEVEAGRMFGPFSHEEVAENYEFFRTSPLGSVVNADGKMRPINNLSFPRNDSEIKSVNSYVDKLDFTTTWDDFTIVSTFFRSHSELFELALFDWAKAYRQIPTRESQWKYLMVKDLHGRLYVGTRITFGGVAGCGSFGLPADAWKQIMQSEFDVVKIFRWVDNNLFVKQKNSSCDMKSIAERSVKLGVATSVEKCSEFAEEQKFIGFIWNGVSKTVRLPDAKLEKRKEQIREYLQPMNEFKLNQVKILAGRLNHVSLLLPQLRCYSRSVYRWMNKWKKKWATRAPPEDVIEDLLFWLSTLNSYSSTRLCPLLDPTKINWVGDASTSFGIGVLIGRRWSQLRLKENWSDASQKRTIAWLETVAIRIGILMLLSIGTNLKGRNFIVWTDNTTTENTLRSRKSRDFHSNNEWKQIQKLLITEDLDITPSRVTLAENISNGLSRGVQRPHVRENRVWFDIPDDLSTFMFHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>H6QT83</accession>
    <name>H6QT83_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178308">
        <property type="protein sequence ID" value="EHS64037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889286.1">
        <property type="nucleotide sequence ID" value="XM_003889237.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QT83"/>
    <dbReference type="GeneID" id="13541649"/>
    <dbReference type="KEGG" id="pgr:PGTG_22001"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22001"/>
    <dbReference type="HOGENOM" id="CLU_053015_1_0_1"/>
    <dbReference type="InParanoid" id="H6QT83"/>
    <dbReference type="OrthoDB" id="2506530at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324:SF2">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="308"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="332" mass="36731" checksum="A0AE2288AE078AC3" modified="2012-04-18" version="1">MPPRKKKNPPAPTPTPTPAQSRPAAPKRKQPVSWEKDGVDGFSSIRILIEWLTSDGNFKRWRGKKGNGLNKEALASEVVALMVSHGIHHRNNKDIRTKIQELQDNYSSACNWLRNTGSGVLDEDIANGTDNVRAAVIKRCKYFYDLDEVMRERTCAIPEDIVDTTSGDVPDIINPPSEGPEGVLDPLIDSIGEGNKGSDQDGSVADSGTAAKSNTKAPFKSKKPKKSTLPAGLEKAIQDTSDYRVKCLQSKERRDAKRLKLERCRIRIEESDAQVRRVLAEAEQAKLRISCMKELKESGFGDEDISKFLQQQFGQGPSHQSDSESSDDVDTD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTG7</accession>
    <name>H6QTG7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22091</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EHS64182.1"/>
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        <property type="nucleotide sequence ID" value="XM_003889149.1"/>
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    <dbReference type="AlphaFoldDB" id="H6QTG7"/>
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    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="86"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64182.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
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    </evidence>
    <sequence length="175" mass="19205" checksum="8FEECDD83B93C32D" modified="2012-04-18" version="1">MASTRSSQAKRKTPNRFQSSISAFNKQGKQPPTTRTKKSAAKSTLSKKKSSKSKKQQTSNSSSQGHRSPSPTQTQGAQNNPDPESSDSTSNSSSSSDSDSGSPLIGFTLDNFQSKLSSWSANQLRKTLQQKKNLSNRVPVDVQEALQLLQQNYLKIKLMLALIRNIGEKTVNKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>H6QU02</accession>
    <name>H6QU02_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mediator of RNA polymerase II transcription subunit 13</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Mediator complex subunit 13</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_22252</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the SRB8-11 complex. The SRB8-11 complex is a regulatory module of the Mediator complex which is itself involved in regulation of basal and activated RNA polymerase II-dependent transcription. The SRB8-11 complex may be involved in the transcriptional repression of a subset of genes regulated by Mediator. It may inhibit the association of the Mediator complex with RNA polymerase II to form the holoenzyme complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the SRB8-11 complex, which itself associates with the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the Mediator complex subunit 13 family.</text>
    </comment>
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        <property type="protein sequence ID" value="EHS64416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003888966.1"/>
    </dbReference>
    <dbReference type="STRING" id="418459.H6QU02"/>
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    <dbReference type="KEGG" id="pgr:PGTG_22252"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22252"/>
    <dbReference type="eggNOG" id="ENOG502S3V9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_230464_0_0_1"/>
    <dbReference type="InParanoid" id="H6QU02"/>
    <dbReference type="OrthoDB" id="103819at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009401">
        <property type="entry name" value="Med13_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46818:SF1">
        <property type="entry name" value="CHROMOSOME UNDETERMINED SCAFFOLD_125, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46818">
        <property type="entry name" value="DOMAIN-CONTAINING PROTEIN, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06333">
        <property type="entry name" value="Med13_C"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0678">Repressor</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 8" id="KW-0805">Transcription regulation</keyword>
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    </feature>
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    </feature>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
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    </feature>
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    </feature>
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    </feature>
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    </feature>
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        <location>
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            <begin position="2009"/>
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            <dbReference type="ARBA" id="ARBA00004123"/>
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            <dbReference type="ARBA" id="ARBA00009354"/>
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            <dbReference type="ARBA" id="ARBA00022491"/>
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            <dbReference type="ARBA" id="ARBA00023015"/>
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    </evidence>
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            <dbReference type="ARBA" id="ARBA00023159"/>
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            <dbReference type="ARBA" id="ARBA00023242"/>
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            <dbReference type="RuleBase" id="RU364134"/>
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    </evidence>
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF06333"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EHS64416.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="2269" mass="252413" checksum="8E60DD0D060FD9D1" modified="2012-04-18" version="1">MSPGSPSNSKNYNSNNSNNNSNNRFYQQQQQQQQYHFQQQQQQLQYQNQHQQFLFQQQQQNYHRQQHSLLHHHHHHHHQAYQQNTINPANQQQQLTPRLQTNPSYPQPLIHHRQLQKNNSSSSKQHSPNYLRSQAQLIPNGSPLPLPPPPIIPQTVSTTTNQSNRHHHHQQQQQQHRQNHHHRDSNRRNYRQSSFRTNLTLPHHPSLPKPPNPERSQTIPLLSLTNNQTQAHQQQQQQQQQQQQQHPHPHHPHHHPQPSTSSTTTPTTTPTSSSFSKSNTNNPNSSTHIRHIHSPSTSTLQSSLPPDEQLGLGGPQAAGYFSPSAIELNPWRGCSPCKWKVPLLNTGLPNSPARDHTNLATIDEELDQTHSSVSHLEQDEWKIRWAKLSLVAQQEASHDQLKPQIANQLHSLRLHFVFDSEGQQSVQGLVVWVYEIPGRLGPKQSYEQPPPLIDPILKTYQTFENHTPIINQPSPIKACQSVISYGVLSYSQLFPNLFTRSTTSNSKPLLPHLSHPAKNRTANDFLVDDSSQTNTIPAYANLLRALNQHIITLAVQSPDSTPFPGACPTRWIRWGPDRLLSVPRDDSLEHLYRNLNRDSTRLMGAILTTAPALVVELKSWLSSRVAMCQPSFTARRLAPMPPLPQHNIPPGFPLILAPFPNLRAQYVRMFGRFNHSLVRCKSGTSSQSSNFSSTRSSWLSDSPSIPNASWISQQKRICRAELKHFEKLKQTWRLAAPFMEAYPETHPTSATNANQRNFESWVVCKITTNNSEAVNPETNDSQEIELVWPVDHCLLDLESWIESAEQNPPPPPTESDPPASLTPSSRICFPRARLANSLMISAYEPTDELAENAGSYIDWVAQERELQRRAAAAAGKEKTPQKTESPPSMTGGRAGLGLNRGRGSGYPSPRFQRHTSTSTARASPVPPHLITPRMSKKRLRDDGDDRSSSQNSTIFPPSIIPFDCGIVTASDRGDSATVDFASNGLSDENGMDIYHDSTATNLPDPPTSQPQEECSISTVIRDEQQPLSNDTLHQSSSVVLAPSSQPGTTSYQFVSAQQGSGSGSTASTISYNTNLFEQPTPGSVSISGLTPGRLNGFTPDANNNFNNNFNNNNNNHPSTQTAHYSSPINQNHHSTTTDPAGPSEADQLAISENALRTLSLMSSSDSGPSDKLFRFVEPVGYESVRFSHTHTVVDDHYTRRGGKYALPPSPHSFSSPEDEQGDDALDIVDQEVLRRARGPLLVHVSKKLMRAIDPRAKVARTLFNSAFRTSDSQQSFSRAEPLTSSAIALTTQGGTGYTESPATPRSWVPIGSSNDSSDGSTFSEDEDEPDGNEFIRSIDARDRGSYSSTTSSCSSDDERIMAIDREYQHAIAAKIEQIERSLKDVLEIQYRSKFFSIQPSSNHSSRPPQNLSPEDRTIKPKHIPPAVRKKLKNWVSQLATQLEHDPNLEVLLETVLPSTSSPKVTTNDTRKTTEDYRVVLDLASMPIINGFDYYRPLVDDEYEIQENRTQNSTTQKKKAEEEEEKEEKKKLGKQQMKVKEVEEGDQRPYLIKKFTKAPASIVVKINEGKQKIAFSLISQLFKYWIKLGFEPVSGQRDSVLSVIFPRPLPRHAHQPKTNSKDPSLVDSGAETLFVNSQQDKHPLSHLNLPSIHHWLLELAHTYKSCRLGELSIGKIHEKMKSLGVGEREIVGLDGRPVTRLLISRHQLLDDRPASLISFAMRLYDSMKIHVHRFTAPGLSFDPYLTPAPIRPGECQPPSESEDSSSDAGELPDRAGQLHHSTDEHKQPRTDGRSRHRRPSDRKLLSGHAFFLTGIQKPTQLNIRWPPSGIDVSARHRMLHIGYLVGPQRAWVLVSMVDELGQQHQLVLKFPASPASSDSSLPSHAPAQKQKASLSRELDEHRLEMQIVSSVWKVSRKIILSTNVEWRVVITKLGRIKPIEYEAWCELLSQTLPASEVAFHVTLSSVMMSDVPMLRRVGAGEGPSTTRLSTTRRESVTEGGGGGEGASDLEMGEVEGPEEGEEGVGDRAEGVEEGGGGGGVEKLGGGPEPVWVVHESTLRPHSSFDPQSPSSSMPRSVNATIVHSLSSSYIYSHQCPVSPGSLDSFPSGTTPSGRGGRFRVDLLGTAASPASVYQANLFQHREEIVHSLVLLSRLKHLRSSSSSSSQHHPSHSGPSDRPSTDVDVDEDDDETKAAGADLALLPGHLSSLIVLARLLNSHLHRLSDLFLLDQTHLPSALLSPSAFKQHDLDGQLDPFNHQLDQLDPLILLHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>H6QV85</accession>
    <name>H6QV85_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22674</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178384">
        <property type="protein sequence ID" value="EHS62786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888579.1">
        <property type="nucleotide sequence ID" value="XM_003888530.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QV85"/>
    <dbReference type="GeneID" id="13540769"/>
    <dbReference type="KEGG" id="pgr:PGTG_22674"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22674"/>
    <dbReference type="HOGENOM" id="CLU_2741261_0_0_1"/>
    <dbReference type="InParanoid" id="H6QV85"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS62786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="71" mass="7808" checksum="FFBA19F8C2EC76B5" modified="2012-04-18" version="1">MGCSFILAVQPPLVQLSARYVRLRPLILANRLLDEPLPVFGPAIFESGYTVLARKSLIYVLQPLSSSHPSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>H6QVP9</accession>
    <name>H6QVP9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22820</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS990051">
        <property type="protein sequence ID" value="EHS63697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890852.1">
        <property type="nucleotide sequence ID" value="XM_003890803.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QVP9"/>
    <dbReference type="GeneID" id="13541132"/>
    <dbReference type="KEGG" id="pgr:PGTG_22820"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22820"/>
    <dbReference type="HOGENOM" id="CLU_1403079_0_0_1"/>
    <dbReference type="InParanoid" id="H6QVP9"/>
    <dbReference type="OrthoDB" id="2498236at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="231" mass="24747" checksum="322A3B6169BBCF02" modified="2012-04-18" version="1">MAGGENGAYACSSQWITPEIVDYTISNSIPAPQYQSIMFSAKQLITALTLALLSVHLVESMKSQCGQWAMQKLSSSSDSDKVVVAKLMSAKNERAFLQRRNDPPGHKGKTVSGGSTSACGGKAYSDNQQGSCLWNGANQDSSPRQGLYRGWLNGANTNNCFKTLWINGAGPLPKYVPVLDGCPFEDYNIKPEVGCATIWVTKATLKALGGKDGDTEVPIKNWGFSADTPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQP6</accession>
    <name>E3JQP6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SWIRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_00651</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307701.1">
        <property type="nucleotide sequence ID" value="XM_003307653.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQP6"/>
    <dbReference type="STRING" id="418459.E3JQP6"/>
    <dbReference type="GeneID" id="10546775"/>
    <dbReference type="KEGG" id="pgr:PGTG_00651"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00651"/>
    <dbReference type="HOGENOM" id="CLU_1982638_0_0_1"/>
    <dbReference type="InParanoid" id="E3JQP6"/>
    <dbReference type="OrthoDB" id="3244016at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00167">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:001141">
        <property type="entry name" value="SWI/SNF complex subunit SMARCC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001005">
        <property type="entry name" value="SANT/Myb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017884">
        <property type="entry name" value="SANT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007526">
        <property type="entry name" value="SWIRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04433">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51293">
        <property type="entry name" value="SANT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50934">
        <property type="entry name" value="SWIRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SWIRM" evidence="2">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="SANT" evidence="3">
        <location>
            <begin position="70"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP74695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="13931" checksum="2576029F8B7C8373" modified="2011-01-11" version="1">MAGGIPPGHQDSVLVAGRDSSRPLRLYCLNPSEKYLTVTACRQNLAGDVCAIMRVHAFLEQWGIINYQVDWEKIVQHVGGTKTKEECILHFLRMPIEDEFLLNSNAKLNTSLVLGLGKIPRAQTGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3JSC2</accession>
    <name>E3JSC2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_01440</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP74847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319266.1">
        <property type="nucleotide sequence ID" value="XM_003319218.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JSC2"/>
    <dbReference type="STRING" id="418459.E3JSC2"/>
    <dbReference type="GeneID" id="10546963"/>
    <dbReference type="KEGG" id="pgr:PGTG_01440"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01440"/>
    <dbReference type="HOGENOM" id="CLU_030586_1_0_1"/>
    <dbReference type="InParanoid" id="E3JSC2"/>
    <dbReference type="OrthoDB" id="2505141at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP74847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="357" mass="40368" checksum="DE3B1DD456AB492E" modified="2011-01-11" version="1">MPWGLGTKKNGKLKASEWHTLFAIHLPLASLDIFLESGNIKEDLRLNGTALDNICALVRCTNLVSSKKITRKDANCFQDEYEVYTATSEKLFPNLKILPNHHYALHIPQHMNWWGPLMAVSKFPGERLIGSLQKCKTNTHQKHTGVTIMNKFCQRQRLKAAHGLDCARKQGDKKQVEAPFEMDRMAYDQVLHFCRQTVPDLRAYYDLPHPPEAKVMFSLAKEIRSLEVGQGRRISKRKPNDIVEYISKDGELAYGQVCDIIRPTGIGHEHSDVIKVIRGKQVARGDAFPVYLEKLNVLQLHFGTIEYIHPPQVVSPVAYRRLPAWSLATVNPSYLIRPLGGLSSAPSSTNDPMELDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUZ3</accession>
    <name>E3JUZ3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01199</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320287.1">
        <property type="nucleotide sequence ID" value="XM_003320239.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JUZ3"/>
    <dbReference type="STRING" id="418459.E3JUZ3"/>
    <dbReference type="GeneID" id="10532783"/>
    <dbReference type="KEGG" id="pgr:PGTG_01199"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01199"/>
    <dbReference type="eggNOG" id="ENOG502SS5M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026730_0_0_1"/>
    <dbReference type="InParanoid" id="E3JUZ3"/>
    <dbReference type="OMA" id="WARSWHA"/>
    <dbReference type="OrthoDB" id="1077582at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="436" mass="50222" checksum="1D40A07C3BB056CF" modified="2011-01-11" version="1">MGQDYSALFNQPMIQQEWAISVKPVVLPVLTSIFLLFISAVSLPCKGRSAVYFRLALLPFQLILTHDICVNKKYSLGSAFRDSSFPTFGFLLLYRALDFSILNLWDPEPAFHWIVPDRPPTTESKSKSPDEKPIRWRKVPHPPLFSFDRMLWALDNLTLMRPGTPFLFPWKIRALEWSQYALESPQTKFGEPEWPLLPAIIQQLVHLGCHLYVRQLDLKAGQTIWTLPLFDQLALTFALGGAVTFSYALEEAIFFPLILRFNLLPKTALISHSKRSIASAGLGELWGKRWHHVWKRCFVRLPRLMPGSSHRLVQMFGAFWTSAMFHCLVLGRLYPTPDISRPISFLPLIYEPGIYKFFLCQGAGVIIEKILLGDPKKDERLTKRIIRIFYLYFFLLGAGRYSTNSLAVKGLLDRDQWNTVTIPAISRMFYDIFFAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3JV09</accession>
    <name>E3JV09_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GH26 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_01215</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 26 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320303.1">
        <property type="nucleotide sequence ID" value="XM_003320255.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JV09"/>
    <dbReference type="GeneID" id="10532799"/>
    <dbReference type="KEGG" id="pgr:PGTG_01215"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01215"/>
    <dbReference type="eggNOG" id="ENOG502RZD6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043977_2_1_1"/>
    <dbReference type="InParanoid" id="E3JV09"/>
    <dbReference type="OMA" id="DIYASKT"/>
    <dbReference type="OrthoDB" id="428177at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016985">
        <property type="term" value="F:mannan endo-1,4-beta-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006080">
        <property type="term" value="P:substituted mannan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022790">
        <property type="entry name" value="GH26_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000805">
        <property type="entry name" value="Glyco_hydro_26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40079:SF6">
        <property type="entry name" value="GH26 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40079">
        <property type="entry name" value="MANNAN ENDO-1,4-BETA-MANNOSIDASE E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02156">
        <property type="entry name" value="Glyco_hydro_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51764">
        <property type="entry name" value="GH26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173270" description="GH26 domain-containing protein" evidence="5">
        <location>
            <begin position="29"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="GH26" evidence="6">
        <location>
            <begin position="65"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="190"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP75884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="351" mass="39725" checksum="337980921D3B1750" modified="2011-01-11" version="1">MGSHLLAPLQRKIIGLFCLVLFAQSLVAVPDASYHNYKSTPKNSHQVIKSRGQNLRQTKFSYLGPAGIRAHDSLTAKIDGVYIGLIPDAGDTGGNVQTMREIIKSINGKRPAVFGWYAQVSVHKEFDGSQLFEILDDLKKSKCVFSPAIMPQGGWAGLRRGDNHQAVAIAKVLRKFTDQEIPVILRFAHEVNWYQRTHEYPGNAADFKEAWAVLAAAIREHAPDVLLHWCPNVSTMEEYRKYAPDNMDYTVDIVGFDYYPKSKPQPDEFVKKAKEFHDAFAINGRKFAMGEAGLHYQASYKDRVDWLSQMAHARKELPNLLFINWYNSKKEYDYKIAGNGLSTTLLTEKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWB7</accession>
    <name>E3JWB7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_02783</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320761.1">
        <property type="nucleotide sequence ID" value="XM_003320713.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JWB7"/>
    <dbReference type="GeneID" id="10534234"/>
    <dbReference type="KEGG" id="pgr:PGTG_02783"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02783"/>
    <dbReference type="HOGENOM" id="CLU_009065_3_1_1"/>
    <dbReference type="InParanoid" id="E3JWB7"/>
    <dbReference type="OMA" id="YACDGAA"/>
    <dbReference type="OrthoDB" id="2507437at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP76342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="528" mass="59094" checksum="F672FACDA5A94F3E" modified="2011-01-11" version="1">MHLEKATKEDDQTFWKLFAAHAASGKFDNLKPFKGLVMAVSVRHERESVGKSLTGVQFSPQFDDFMMTLAAISPRGAHFFQETFAGRSLRSQRDIRAKTSMQLTDGLSVINFERISSILKTLDYSGPLAVGSDQTVCLKSLRVHNGFLVGAQGGDIKFDSEEQLKSLTQDIVKSQNFCPKLRAYTIQVPLPGIPTYVVALLASKDKESSSDIVETHTKFVKMCDKSGMKILSLASDAQMDLTKISDAHLTFVRPKYNVNIKLPLIGNPPRPLVGVQDPKHARKTCVNQILSGARLLSFGKYWISILHLSTIVENQDSSLYVKDVFNSDKQDDGWAYCVFNEDTLKIALQNNDCTGLALYLFYLVEQQKATSGVMSLNRNGISYPSQKILASLADSLLGLIISHREYYANVPLMPWKHGTEACEHIFGWMRVILPNFTVLDARQMMPKIFIIVKSIMSGKVTDTPFQNLVSRKIDDLLSIARKRAVWKVRRALGDFLYFSAPQHVLLRFVVRGNLLDGLVGNTLTLVYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWI5</accession>
    <name>E3JWI5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7872 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_02851</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76410.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320829.2">
        <property type="nucleotide sequence ID" value="XM_003320781.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JWI5"/>
    <dbReference type="STRING" id="418459.E3JWI5"/>
    <dbReference type="GeneID" id="10534465"/>
    <dbReference type="KEGG" id="pgr:PGTG_02851"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02851"/>
    <dbReference type="HOGENOM" id="CLU_465498_0_0_1"/>
    <dbReference type="InParanoid" id="E3JWI5"/>
    <dbReference type="OrthoDB" id="2501761at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057194">
        <property type="entry name" value="DUF7872"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33339">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33339:SF1">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25278">
        <property type="entry name" value="DUF7872"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="368"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7872" evidence="2">
        <location>
            <begin position="400"/>
            <end position="619"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP76410.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="621" mass="68578" checksum="FC4D1D2250BE11E0" modified="2012-04-18" version="2">MNCPLESVVVRSKEIAVAPVARGVSLKGIPRTPGCSMRIQATLQNARILVDVNAFPRPSNTVIMVKSLRILCCYAFELRCRRLGLHTGSDLRPEARESLNHLDELTRSSSMNLIMWIGSVSELYVTTGSSSHSHPILRTTDPRMQARSFSHSRIYVSKTLLMQVILQILLILILPKANGHALQVYSGRQNSSPRHVLHARQELFQDGGVGYKLPLGAKPFQPYKCGRKPLSPKTWKELRIDEYIKTYPRGLVANLTVFAAENKAINFACGMNQFCSAGQLCSPISGRAWWVLAAVEQATMYFNSLSTAIGFAAGQAKAIGAELVNDLYLQDAKKKYRLFQSIAMVLTMALAVTAMIAAVVFMTVTGTVAFAVFATAGAMIAIAQVTMILKAQAEEVEAGNQDTFTKWSHYEGRISDWQSKSQTEIGKRVKDMVEYPISSHRGLFGLLEGGEYCRNNQKQDSNDLEKKMARILTIRFANQILRAKKGFITIGEGCYSKGPNGAFSLDEGWLSYCDKPGGLMYNVIYDDDGKSGNQFYNARLLVEKYHISVEYIVKQAVECSQLGKGPDYDPYADGTNLPADENARCVMNLPVCDTRGAGVRKMLKHHTTVKACRVAGGINLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWN3</accession>
    <name>E3JWN3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_02899</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320877.1">
        <property type="nucleotide sequence ID" value="XM_003320829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JWN3"/>
    <dbReference type="GeneID" id="10534515"/>
    <dbReference type="KEGG" id="pgr:PGTG_02899"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02899"/>
    <dbReference type="HOGENOM" id="CLU_124024_0_0_1"/>
    <dbReference type="InParanoid" id="E3JWN3"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000612">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051826">
        <property type="entry name" value="E3_ubiquitin-ligase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765:SF450">
        <property type="entry name" value="ERAD-ASSOCIATED E3 UBIQUITIN-PROTEIN LIGASE HRD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765">
        <property type="entry name" value="RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="2">
        <location>
            <begin position="133"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP76458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="188" mass="21443" checksum="AD6C98AD54C16B8B" modified="2011-01-11" version="1">MDAEMLERTEEALSHLTPQDRQLFERHLRSLQAAHDQLAAHPDRDRLDDDENRRYFSDRDEIEAALARLPEAVTQRLRHVLGLWEVLIFFLEESRACSFGIRRRLAQQLSQFTLSNTAAATLTTSPDGEALECTICNEELSLPDQSIVQLPCHASHLFHRDCILLWIGRDRTCPICRAAIPLSSDPAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXF3</accession>
    <name>E3JXF3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">GH26 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_02189</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 26 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76728.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321147.2">
        <property type="nucleotide sequence ID" value="XM_003321099.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXF3"/>
    <dbReference type="GeneID" id="10528749"/>
    <dbReference type="KEGG" id="pgr:PGTG_02189"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02189"/>
    <dbReference type="eggNOG" id="ENOG502RZD6">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043977_2_0_1"/>
    <dbReference type="InParanoid" id="E3JXF3"/>
    <dbReference type="OrthoDB" id="428177at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016985">
        <property type="term" value="F:mannan endo-1,4-beta-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006080">
        <property type="term" value="P:substituted mannan metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022790">
        <property type="entry name" value="GH26_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000805">
        <property type="entry name" value="Glyco_hydro_26"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40079:SF6">
        <property type="entry name" value="GH26 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40079">
        <property type="entry name" value="MANNAN ENDO-1,4-BETA-MANNOSIDASE E-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51764">
        <property type="entry name" value="GH26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172216" description="GH26 domain-containing protein" evidence="6">
        <location>
            <begin position="19"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="domain" description="GH26" evidence="7">
        <location>
            <begin position="169"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="77"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="89"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="99"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="116"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="153"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="4">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4">
        <location>
            <position position="396"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP76728.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="460" mass="48943" checksum="5CA0ED0759D0F35A" modified="2012-04-18" version="2">MFLSSLFVAFLPVLGAICDEFANDDVRSGATSHASSTSRRYRHRKVGSMSEKCYFFDVGPWRSIPLYGIRCHHERISRSPARSGGRKTAPQTQANQTDTSGNTTASVASANTTVSPDSSTSAPVTPSDNSTALQNSSTSASSADVSDNSTASQPSNTSTVSTPVASGNAAAPAAVMGLDSSHLSHNGLSLGFLPDGGDAGGTRQTMADINSAVGQVSSVYGWYGQAVSGTLFDGAQLTAVIDDVKASGAVFQPAVMPVQGWKGLTSSDNSQAVAIAQVMKKFTDQGIPVWLRFAHEMNYYQTDGTYVGNAADFKAGWAAVAAACKSIAPDVKMWWTPNVASAENYAEYEPDDMSTVHLVGIDFYPKSLQTGKEFLDTMKPFHDKYAVDGRMFAIGETGLGYAGTPAERFSWLSQVTAARSEMKNYVSAAWFNYLKDYDYQIVSLKTPSLTSDLKSFLAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXQ3</accession>
    <name>E3JXQ3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Dolichol-phosphate mannosyltransferase subunit 1</fullName>
            <ecNumber evidence="13">2.4.1.83</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGTG_02289</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Transfers mannose from GDP-mannose to dolichol monophosphate to form dolichol phosphate mannose (Dol-P-Man) which is the mannosyl donor in pathways leading to N-glycosylation, glycosyl phosphatidylinositol membrane anchoring, and O-mannosylation of proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a di-trans,poly-cis-dolichyl phosphate + GDP-alpha-D-mannose = a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate + GDP</text>
            <dbReference type="Rhea" id="RHEA:21184"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5 13">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Component of the dolichol-phosphate mannose (DPM) synthase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4 13">Endoplasmic reticulum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 13">Belongs to the glycosyltransferase 2 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.83" evidence="13"/>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76828.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321247.2">
        <property type="nucleotide sequence ID" value="XM_003321199.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXQ3"/>
    <dbReference type="FunCoup" id="E3JXQ3">
        <property type="interactions" value="468"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JXQ3"/>
    <dbReference type="GeneID" id="10528931"/>
    <dbReference type="KEGG" id="pgr:PGTG_02289"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02289"/>
    <dbReference type="HOGENOM" id="CLU_739956_0_0_1"/>
    <dbReference type="InParanoid" id="E3JXQ3"/>
    <dbReference type="OrthoDB" id="2603at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004582">
        <property type="term" value="F:dolichyl-phosphate beta-D-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006066">
        <property type="term" value="P:alcohol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006488">
        <property type="term" value="P:dolichol-linked oligosaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006720">
        <property type="term" value="P:isoprenoid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06442">
        <property type="entry name" value="DPM1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000036">
        <property type="entry name" value="Dolichol-phosphate mannosyltransferase subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039528">
        <property type="entry name" value="DPM1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43398:SF1">
        <property type="entry name" value="DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 13 16" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="15">
        <location>
            <begin position="143"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="93"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00006739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU365083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EFP76828.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="374" mass="42407" checksum="E4D573757C2F8E7A" modified="2012-04-18" version="2">MRWDCLFTIGYFRWKEESGRRFLSSLVQLPAMKEVSKLLLEGNSVRMTKLSQDQENTRSLSDKPSEAVKREIRSLGCPARARDVIEDGASAPLEHRGKLSSPELPCPQSSVTNSNTTLNTTAQTRHILMIRSEGDMESKTNDVILPTYNERRNLPIMIWLLARTFDQHQIDWEVIVVDDNSPDGTLEVAQQLQRLYGPHRIIIRPRAGKLGLGTAYMHGLSTCTGTFTIIMDADFSHHPKYLPTFIALQRAHGLDIVTGSRYRPGGGVHGWDFKRKLVSRGANMLTYILLDPGVSDVTGSFRLYRTSILRELIQKTTSRGYVFQMEIIVRARSMGCRMGEVPITFIDRLYGDSKLGKDEILGFVKGLWKLFWTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXV1</accession>
    <name>E3JXV1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02337</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321295.1">
        <property type="nucleotide sequence ID" value="XM_003321247.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXV1"/>
    <dbReference type="GeneID" id="10529061"/>
    <dbReference type="KEGG" id="pgr:PGTG_02337"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02337"/>
    <dbReference type="HOGENOM" id="CLU_074641_0_0_1"/>
    <dbReference type="InParanoid" id="E3JXV1"/>
    <dbReference type="OrthoDB" id="10332536at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="323" mass="37949" checksum="2F53CB41534C18D2" modified="2011-01-11" version="1">MTQLDSFSRKEQVNFHNPAAEDNPTSSELIADKSRSINKDFHMVLELFEDHQNPKEEHGWVTREITHKEFAERTRKQAEILRDLKLLELGKFDLISKEILESTLKDHLQDCDIQTWLGDSTPDIAIVAKDEGKKLGLSLLDQIKKEVVDYKVLIPFSDAEFEDYLICNTSKYLPYLFETVDFLWKNEFIDQECLSNISEDEKFLRRASLYTRKYYFSKLNVMSHEYGTNITKQWFAPLGFQFIDALNAQQQARAKLQLITANMMQISNYYEFPFRSPEAQAAKEFKELLSSQRYSNYLKSGKQLSNLRYSNDLHNENLELKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3JYS3</accession>
    <name>E3JYS3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP77198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003321569.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JYS3"/>
    <dbReference type="GeneID" id="10537574"/>
    <dbReference type="KEGG" id="pgr:PGTG_03154"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03154"/>
    <dbReference type="HOGENOM" id="CLU_1019898_0_0_1"/>
    <dbReference type="InParanoid" id="E3JYS3"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="119"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="239" mass="26817" checksum="3B642CE3C57B9596" modified="2011-01-11" version="1">MTTYFEIPDRFVGQPLSPYSLASDESTLPDEVSAPDEDCSVPPGDDPVVKAEDTEVPMPGSPIDTRRRVEDHIPAPRPPPPSFSDIRLFAIAAAFQASSDGQFLFHYQERHARSPSPPRSSEYSGSGSASSPIRVDTPPPAEPCYVYPPHPNPDVKLTPEERGPIWAEVVQEDAVLNARRHIKTFFRSYFELLDGDVLNHPRRSGGYRRLARCRLYENVMEELDRVLETVDQVQMTVIE</sequence>
</entry>
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    <name>E3JZG6_PUCGT</name>
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        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the KTI12 family.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003321812.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZG6"/>
    <dbReference type="FunCoup" id="E3JZG6">
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    </dbReference>
    <dbReference type="STRING" id="418459.E3JZG6"/>
    <dbReference type="GeneID" id="10538319"/>
    <dbReference type="KEGG" id="pgr:PGTG_03397"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03397"/>
    <dbReference type="eggNOG" id="KOG3062">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027147_2_0_1"/>
    <dbReference type="InParanoid" id="E3JZG6"/>
    <dbReference type="OrthoDB" id="9972657at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="KTI12/PSTK"/>
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        <property type="entry name" value="P-loop_NTPase"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF08433">
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
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    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="178"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="194"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP77441.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="349" mass="37947" checksum="FDEF874ADD826E83" modified="2012-04-18" version="2">MTSYWGCLALRTMVSCSVKPGWSWLLMGSNLTSISRITESKTEKVARASLLSSTIRKLSKDHIVICDGMNYIKGFRYQLYCAAREAGVRTCTIHVANLQANCLAYNAKLPPESCYKEETIQNLFTRYEEPNSSVRWDSPLIVFPWCDSLVSTADDGVAGDNAPGLAHKLQGLRVLGNDGEEQDSTTARPLGSEPPNQSAAPEGIKSSQSTKEGARWSRIAEDIWTAITKGDLKPVHAAVKTATPTSTNFLHVLEITTTSVLTSLLSAVNESQNRCGLVSFPCVLPHSGKSCVFSINIPMGKTVNVGVLQRLKRQYTTMHKQANAAGRGTSSHDEDGIADSFVKYVQGAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3K0N7</accession>
    <name>E3K0N7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Velvet domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_03818</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322281.2">
        <property type="nucleotide sequence ID" value="XM_003322233.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K0N7"/>
    <dbReference type="GeneID" id="10540847"/>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="entry name" value="Velvet_dom_sf"/>
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    <dbReference type="PANTHER" id="PTHR33572">
        <property type="entry name" value="SPORE DEVELOPMENT REGULATOR VOSA"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="VELVET"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Velvet" evidence="5">
        <location>
            <begin position="24"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP77862.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="204" mass="23194" checksum="8785ACE10329D2F0" modified="2012-04-18" version="2">MFGDIRLFLGASSGGNNAFERKNMCQPTYELRIIRQPHFGAQTTEEQAAQNTGLPIVPVPIIQVIRRINGEEVPLELAEAMRLFLTPHAVRVAEDGRLVEAPNQGLRFEPTSPIMDTQPEIADDQGRWLFVFGDIGIRDLGRYQIRFTLWQAGQDSSDLLAQVSTETFPVVERDDWPLHNDWDTPLSARLARDHPFARIYRPPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3K0U4</accession>
    <name>E3K0U4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03875</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP77919.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003322290.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K0U4"/>
    <dbReference type="STRING" id="418459.E3K0U4"/>
    <dbReference type="GeneID" id="10541065"/>
    <dbReference type="KEGG" id="pgr:PGTG_03875"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03875"/>
    <dbReference type="HOGENOM" id="CLU_376039_0_0_1"/>
    <dbReference type="InParanoid" id="E3K0U4"/>
    <dbReference type="OrthoDB" id="2507832at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053019">
        <property type="entry name" value="GATA_zinc_finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23353:SF23">
        <property type="entry name" value="PROTEIN HAIRLESS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23353">
        <property type="entry name" value="RAB-GAP/TBC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <end position="418"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="505"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="536"/>
            <end position="603"/>
        </location>
    </feature>
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        <location>
            <begin position="9"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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            <end position="75"/>
        </location>
    </feature>
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        </location>
    </feature>
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            <begin position="129"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="177"/>
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        </location>
    </feature>
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            <begin position="250"/>
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        </location>
    </feature>
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            <begin position="260"/>
            <end position="270"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="349"/>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
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    </feature>
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        <location>
            <begin position="541"/>
            <end position="579"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77919.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="603" mass="66896" checksum="AA3A43AABBC672C9" modified="2012-04-18" version="2">MSEGDQHNPVASTSKNVISDQNPIHTIDSDLTKLRQLRLTITQQQEHQYQKHKNQQQHPSTPPDSFHSSIQSAESIDQKPNRLLQQRKISHVQPPINAQPIDQQEPQMIQQEQELVTLPVPVENPTAASDRESISRDPRLKKKPLPSQPSSLSGEQPSTQTKDHLDPQPQPSLPEPSEPKPEPADDRSKELQRSRAASERAHSPRPPEEQEERSQNSKSGGEGRRRRSTEEQVSRSRARSTRPSPEPSRGRRSRSRKSSGSREERGKEESNESEQADQADQEEEEEEEAATTTAGWSEARIVAEQRRYEEEMVRYYAELAVYERKMRERHAASRSSSSSSTSASASAFQHHHPQRRATQRRRDSTEEYFQRSLESVADSSARSRRFSVAGDRSTLPDPEPPSVDERMADKPRLEPEQETVEAAAVLLGLGLDRSSISLSVPVGPSTNTKPITGNPSNSSGPKSPSKSTERRDPTRSAIQDILSASAHLSRSDNPSPLEPLLKIPNRTASPNASLLDHLHRPHSPNQAAIRLLQLPHPSIPSSSSSSNSNSNSNLNSNSNINLNSNSNSNLNSNSLRSSLPTRALSGPMLPPGRKKQKLASPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3K1G5</accession>
    <name>E3K1G5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_04096</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP78140.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322559.2">
        <property type="nucleotide sequence ID" value="XM_003322511.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K1G5"/>
    <dbReference type="GeneID" id="10541770"/>
    <dbReference type="KEGG" id="pgr:PGTG_04096"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04096"/>
    <dbReference type="HOGENOM" id="CLU_011079_0_0_1"/>
    <dbReference type="InParanoid" id="E3K1G5"/>
    <dbReference type="OrthoDB" id="10338788at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173149" evidence="2">
        <location>
            <begin position="19"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="94"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP78140.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="490" mass="56713" checksum="2A6D280B3FD04082" modified="2012-04-18" version="2">MVVLSKGLLLCLVWHVTGPNLFALSTLVHEAEESIVFVLCDPLYLLKVTLMSISVSGRPPVVLNDQNAYRLTLLDENELDQHYPSEYGSIPQEQAERKAEIESMKNEHEKIEKRIKQLKALPGQTSKAKALLKELHKNLDIPMKERFLQLKLTPTQSPGTSFNLEADTNQLENTWNVLQTQLIRTVYSKKHVNPAEADFVLEFTKSFFLLGDYIYKYGLLPAPFIKQIEIFHGPTIFDIISRHINYMFRGNQGNFFDHAESVIPQLEFLKTAPSVKHFHRTIKALPDLYDMYVVHWALVSIMLNAKSHFATQSASGRFEAIRSGFVEVTFLNRAQHLLSELKQNPNLNYRLESESISAVNLIGDMANYFQKPPANTLSNQRRLEFQIVFYALDFLDQHCQPLVEAMVRRGDIRSPFRKQLRYMRGYLKFYQNQFHERAGSARVEQDMTFLELSKDGAIRDWIEIIPLDSFEHNRSLRRSPKFSLWMGQKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3K286</accession>
    <name>E3K286_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Expansin-like EG45 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04411</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322874.1">
        <property type="nucleotide sequence ID" value="XM_003322826.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K286"/>
    <dbReference type="GeneID" id="10536134"/>
    <dbReference type="KEGG" id="pgr:PGTG_04411"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04411"/>
    <dbReference type="HOGENOM" id="CLU_026963_3_0_1"/>
    <dbReference type="InParanoid" id="E3K286"/>
    <dbReference type="OMA" id="WASGANC"/>
    <dbReference type="OrthoDB" id="623670at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22271">
        <property type="entry name" value="DPBB_EXP_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.760">
        <property type="entry name" value="Expansin, cellulose-binding-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036749">
        <property type="entry name" value="Expansin_CBD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051477">
        <property type="entry name" value="Expansin_CellWall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836:SF21">
        <property type="entry name" value="EXPANSIN-LIKE PROTEIN 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49590">
        <property type="entry name" value="PHL pollen allergen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="247" mass="27173" checksum="AF5E0B4EC6DEF8CA" modified="2011-01-11" version="1">MYSKLFPSFYITISLSILFLDSLQLIAGLVLTPELAPKTGQKFTNVQGTYWNGATFAGSCLLQSYQQPRNLEYVAVAKDLNGRAEYCGACIKVTPLDGKRAPVVAVVSSYCANCPPGALDMPQTMYNRLMGDGPKRPGVDRFSWEVVACPFQREKPKIINKEGASKFHLSILIADSDFPIASVAIQAGPAARWLLAQKTDYNCWEIPQQPLLPDRVNVKVTCSNSRFFIVNNFDPASRTPLDAPANC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2Y6</accession>
    <name>E3K2Y6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_04262</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78306.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322725.2">
        <property type="nucleotide sequence ID" value="XM_003322677.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2Y6"/>
    <dbReference type="STRING" id="418459.E3K2Y6"/>
    <dbReference type="GeneID" id="10542259"/>
    <dbReference type="KEGG" id="pgr:PGTG_04262"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04262"/>
    <dbReference type="HOGENOM" id="CLU_474975_0_0_1"/>
    <dbReference type="InParanoid" id="E3K2Y6"/>
    <dbReference type="OrthoDB" id="1068471at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140042">
        <property type="term" value="P:lipid droplet formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009617">
        <property type="entry name" value="Seipin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052234">
        <property type="entry name" value="U5_snRNP_Component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44006">
        <property type="entry name" value="U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 40 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44006:SF1">
        <property type="entry name" value="U5 SMALL NUCLEAR RIBONUCLEOPROTEIN 40 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06775">
        <property type="entry name" value="Seipin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0853">WD repeat</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="430"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="154"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="461"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="465"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="509"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="562"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP78306.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="574" mass="64440" checksum="D076B952630EC139" modified="2012-04-18" version="2">MSLSLYRTTNLWSAYGEECRNYGVLKGPKGVILSIDFLPLRPLERLYAGSADKTLTAFDPKTDQVVRRHRSHGVLLNSIAVSGASGQDLLASASDDGTVPIWHKDDKNPLDSFKLGYPLTAVERSANGQPIFVGGLDNDIHCFDLRKKEVFWSLQAHTNTIAGLKLSPDGSFLLSAGFHDTVRIVITSTHDPNAPRLVQTLLGAPAGFDNQLRSPTWDASGDKVAVGATDRTFIKVSPPTSPPLGLFHPPEVPHPLLSVNELGGLPSEEHSLPPLKAHIQITRSRCQNRFIFQRRIRRTSPCRMMPGNCRRYGSLTRPEERLTLVQEAIQDLLNYRKITYDLGQHWLKAFEAICLTQKDFEKHRIIHPSSLGKRTEISHATIDVGRQDVYSSPTLLLERNGWGELQIYKAALVFDAHLEGLRWARYYHPYISFCFFSVFFFIAELMAVFVTWGAALYRQSPSKSPELRPFPKEESYSGRPHSPTNQNRLKHETPALGTTEVDSSSTSENDPRSLHDPEDREHDAFFDEPPRIKTEINEDGVLVGSSRDINTDHSTSDPQGRLIHRKSSKTSRYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3A0</accession>
    <name>E3K3A0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04913</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78957.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323376.2">
        <property type="nucleotide sequence ID" value="XM_003323328.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3A0"/>
    <dbReference type="GeneID" id="10537145"/>
    <dbReference type="KEGG" id="pgr:PGTG_04913"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04913"/>
    <dbReference type="HOGENOM" id="CLU_636369_0_0_1"/>
    <dbReference type="InParanoid" id="E3K3A0"/>
    <dbReference type="OrthoDB" id="2503534at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="85"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="419"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="122"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="307"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="325"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="367"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="404"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78957.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="431" mass="46121" checksum="E28F49414BB9EF01" modified="2012-04-18" version="2">MPEPPKITTSLADRIARLNATQAHSLDRNHAQVQEQIGEIKSKISKYEATGQHEAPLIPKGSFGLGAPLARPDRGLDRMRIASLGVSGGTTVSIKSSPSNSLSASTGPTRSVSLGRPDSRRLSNLSSSDQHSRQSSPTHSPSQPLPSEQLPTPPQETQDPLGEQGAARTKTTKEDPQDPSEDQEANNSFDSAEAYDGILDGYPTTPAEEDCPPMVTCSNCSRSVELELLGDHVCEQSLPPSKAQSPTERSPEGARRPPADVPVDAVESPALVISQDHPEEPPKDQQPPLSPSSSVCSSSKRTHDPSTTNNNTTTTTTNTHLSPAAGPPEPLRPPTPVICNKEKDLDSTHQSNKQGSPEILSPKEPQLQANNRLSDSSHSTNTTRLSTGPIPPSSASAGRRSWYNDDDDPDYYDNENAPTGFVTIVRSSKRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3K426</accession>
    <name>E3K426_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_04784</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78828.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323247.2">
        <property type="nucleotide sequence ID" value="XM_003323199.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K426"/>
    <dbReference type="GeneID" id="10536849"/>
    <dbReference type="KEGG" id="pgr:PGTG_04784"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04784"/>
    <dbReference type="HOGENOM" id="CLU_054992_0_0_1"/>
    <dbReference type="InParanoid" id="E3K426"/>
    <dbReference type="OrthoDB" id="2514325at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172377" evidence="2">
        <location>
            <begin position="18"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP78828.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="319" mass="33695" checksum="FE1F429AF9F22FBC" modified="2012-04-18" version="2">MLSAVYVACLLAASAIASPVQHNPRYVAGSYSQDSAHSNEDHARGASSTPFSSSSYSSDRSNSDASHKSGSYYNGGSPYLGGGAAGFGGYGNYLNGGYGAGGYNGAASAYNSASAYNSASAYNTASGYNGIGSNGYGIGNYDPSMYAGGASNFGSANSFSSSQSQQSYSMSTVNSAFSSLNSYLENMNSRLISGQITQQAIYQVVQQITQYFESAMLQATYCGECVNGQSQFSSVASSSFSQFTHFMNQVERQFGGSIFDQFSNPMSRLSTSMQSFFHRASSSSFSMTSQSFIPSDFTPLAQRLMPDVVPSLSQFQQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3K4T0</accession>
    <name>E3K4T0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05566</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
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    <dbReference type="KEGG" id="pgr:PGTG_05566"/>
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    <dbReference type="InParanoid" id="E3K4T0"/>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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    </evidence>
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</entry>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="InParanoid" id="E3K4Y0"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79295.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="215" mass="23424" checksum="A38376C3A060A4E6" modified="2012-04-18" version="2">MSTRSNNPNLHGPLPPATRPRRSKSAGGTGEPFEIDNNSKETSADSHQGDGDIGSQRLSPDNQPCDDRSGKNRSPSDNSRDQCPRLHGLGSVGAKNASPENGSSDHSFGSDGRGSRSFNLFGGYPATPQVAGEAGDSQKVLQKLIPREELEEFVKGWNPWTIKKELFPTAPKNNDGKKRSSSSKGAKERYNDPKRWKMVMRGCNTLEAAYRHMAD</sequence>
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    <accession>E3K5S9</accession>
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    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <dbReference type="InParanoid" id="E3K5S9"/>
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        <location>
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            <end position="121"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79500.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
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    </evidence>
    <sequence length="222" mass="23131" checksum="B20199BDC0FA8476" modified="2012-04-18" version="2">MSLSSAEVNAATALADVLTNNSPLSSSSSAVSPVSEGIDDGFTLPPLVELRPSAYLQTHAGAILDVDTSTNKDLGFVPLFNAAPATPVLLSSDQEGSIATEDELGPSDSNEDSRLQTLPDSSDRCRKTYLQSNATLIASPTPSEQFVRPTEALTSPGPEPVDYSVVIPAPNGYRSPSVVSFSDLPGNGTADSPYYVNTPPPGSDAEENATDRGPFFAEVLNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3K6P6</accession>
    <name>E3K6P6_PUCGT</name>
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        <recommendedName>
            <fullName evidence="3">Retrotransposon gag domain-containing protein</fullName>
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    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
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            <taxon>Puccinia</taxon>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EFP80042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324461.1">
        <property type="nucleotide sequence ID" value="XM_003324413.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K6P6"/>
    <dbReference type="GeneID" id="10534059"/>
    <dbReference type="KEGG" id="pgr:PGTG_05267"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05267"/>
    <dbReference type="HOGENOM" id="CLU_1366843_0_0_1"/>
    <dbReference type="InParanoid" id="E3K6P6"/>
    <dbReference type="OMA" id="PRDWTRF"/>
    <dbReference type="OrthoDB" id="2495075at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP80042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="200" mass="23640" checksum="A6E97CDC2EABE029" modified="2011-01-11" version="1">MTQAFIHDPDEHFERDVDFVFEGWSYNNCPTYDGHITIDVWRWLETLSFTLEIRVAHPDIWHLVGFRLIKGHALHVLNEALESGNEPRDWDSFCQWAQSLNHLAPHVVNAYNRCMVADDYEKLRQRDNETAKEFCERFSHWQLKAGYHRYTYEPRTAFVDRLNNDLRPRVCSRITDMKLQGLPISFPTVVHAAFNEDACD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3K8L9</accession>
    <name>E3K8L9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06726</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325189.1">
        <property type="nucleotide sequence ID" value="XM_003325141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K8L9"/>
    <dbReference type="GeneID" id="10539790"/>
    <dbReference type="KEGG" id="pgr:PGTG_06726"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06726"/>
    <dbReference type="HOGENOM" id="CLU_1468906_0_0_1"/>
    <dbReference type="InParanoid" id="E3K8L9"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="16"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP80770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="184" mass="19083" checksum="790BC7D56D847AD6" modified="2011-01-11" version="1">MDGHDLNNSGHVARMIHSHKSTGDKTDKQASLTRSSTKSHFSHQPPAKAGRTSAATLGGAEEENNESDFSGSELEVTAGQAEVSLSSTVFSNPSGNANLESGCSVSMTPDLSAISRAKADSTPVRLADHSLVEATHKGVSKLPLDTDATVTTLVVPDLAEPLLSIAGLCDAGLTVVFTKQSEKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAK4</accession>
    <name>E3KAK4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Myb/SANT-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_06949</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81328.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325747.2">
        <property type="nucleotide sequence ID" value="XM_003325699.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KAK4"/>
    <dbReference type="STRING" id="418459.E3KAK4"/>
    <dbReference type="GeneID" id="10536899"/>
    <dbReference type="KEGG" id="pgr:PGTG_06949"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06949"/>
    <dbReference type="eggNOG" id="ENOG502T5AI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044506_0_0_1"/>
    <dbReference type="InParanoid" id="E3KAK4"/>
    <dbReference type="OrthoDB" id="2505558at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024752">
        <property type="entry name" value="Myb/SANT-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46929">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46929:SF3">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12776">
        <property type="entry name" value="Myb_DNA-bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myb/SANT-like" evidence="2">
        <location>
            <begin position="3"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="122"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP81328.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="266" mass="29347" checksum="7C5AC61B1E71065C" modified="2012-04-18" version="2">MEQSALELYVRAVESGKQGEGGFKPEVHPWVASELLKDFPGNPFTSAKVKSKYTQGFKKIYNAFVACKGASGFGWNEAKSMVTASEDVWNSFLVLHPSAKRFKNTPFPEYSDFQTIFEGHTARGDMHQSPGTETNKESAALETADSGNLPETLEATRAPTQRPGVQPPRRHRITSGDRFENSIDQLVDAFISSNTKTESPASSPIHLALEKFQEKFADDLGLDELVAGFSVLESDSKAQCFLAIKNDDHARAWINRQIASKMVPDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBN6</accession>
    <name>E3KBN6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_07956</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81707.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326126.2">
        <property type="nucleotide sequence ID" value="XM_003326078.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KBN6"/>
    <dbReference type="GeneID" id="10533415"/>
    <dbReference type="KEGG" id="pgr:PGTG_07956"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07956"/>
    <dbReference type="HOGENOM" id="CLU_3069761_0_0_1"/>
    <dbReference type="InParanoid" id="E3KBN6"/>
    <dbReference type="OrthoDB" id="10295825at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP81707.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="53" mass="6038" checksum="C45D283F2B25A8A0" modified="2012-04-18" version="2">MGKQKENPQSNKIEAYLIAKLFFKENNLDHKTTPLKLEMSLDIQGRQGGYLGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCA0</accession>
    <name>E3KCA0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_08273</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EFP82024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326443.1">
        <property type="nucleotide sequence ID" value="XM_003326395.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCA0"/>
    <dbReference type="GeneID" id="10534392"/>
    <dbReference type="KEGG" id="pgr:PGTG_08273"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08273"/>
    <dbReference type="HOGENOM" id="CLU_1215306_0_0_1"/>
    <dbReference type="InParanoid" id="E3KCA0"/>
    <dbReference type="OrthoDB" id="10404543at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP82024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="228" mass="25819" checksum="128798AC89044B06" modified="2011-01-11" version="1">MAPNIPNFGFFQQFVACCFALRCTLGKARPAFQSLDKSHYMGSDLHSSSLEVPYNVFNRKRPSGNQQTSNQLDISGFDTHWADLGIHHYLEAVNPTNNENSRVTKRQSTDWHGYDGRIPPPDPDPLALTLGFTDLLTAMEFEVSPTSFLPSASSLIPQERRLFNSENSHLNVLTPESMFESVSRHEMGTNNDDLKISRLFEQLQSPEFENIDAQRSLSAYVMPRCKIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCH0</accession>
    <name>E3KCH0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08167</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EFP81918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326337.1">
        <property type="nucleotide sequence ID" value="XM_003326289.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCH0"/>
    <dbReference type="GeneID" id="10534118"/>
    <dbReference type="KEGG" id="pgr:PGTG_08167"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08167"/>
    <dbReference type="HOGENOM" id="CLU_1644555_0_0_1"/>
    <dbReference type="InParanoid" id="E3KCH0"/>
    <dbReference type="OrthoDB" id="10486687at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="161" mass="16913" checksum="C629A8EABA6EBC82" modified="2011-01-11" version="1">MAQSSKLTTGLFLSCRSRQPIPRSRLQGFFSHAGVAAPNPHILRTSPPPISTPPTGSPPTQPCPTPLRRLHHQKDASNRLQRPNDATKVPAAKAAAEGLPSPRQGCCQRATAKDTSDRPQPEQPVGVVIGVRVAAGATAVIARGTGVTAVELQRCWPVAVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDM6</accession>
    <name>E3KDM6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Replication protein A subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGTG_08418</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">As part of the replication protein A (RPA/RP-A), a single-stranded DNA-binding heterotrimeric complex, may play an essential role in DNA replication, recombination and repair. Binds and stabilizes single-stranded DNA intermediates, preventing complementary DNA reannealing and recruiting different proteins involved in DNA metabolism.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the heterotrimeric canonical replication protein A complex (RPA).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the replication factor A protein 1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326881.1">
        <property type="nucleotide sequence ID" value="XM_003326833.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDM6"/>
    <dbReference type="FunCoup" id="E3KDM6">
        <property type="interactions" value="733"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KDM6"/>
    <dbReference type="GeneID" id="10529841"/>
    <dbReference type="KEGG" id="pgr:PGTG_08418"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08418"/>
    <dbReference type="eggNOG" id="KOG0851">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012393_2_1_1"/>
    <dbReference type="InParanoid" id="E3KDM6"/>
    <dbReference type="OMA" id="DQCDAFY"/>
    <dbReference type="OrthoDB" id="1751331at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005662">
        <property type="term" value="C:DNA replication factor A complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043047">
        <property type="term" value="F:single-stranded telomeric DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051321">
        <property type="term" value="P:meiotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007004">
        <property type="term" value="P:telomere maintenance via telomerase"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04474">
        <property type="entry name" value="RPA1_DBD_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04475">
        <property type="entry name" value="RPA1_DBD_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04476">
        <property type="entry name" value="RPA1_DBD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04477">
        <property type="entry name" value="RPA1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000041">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000064">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000090">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000117">
        <property type="entry name" value="Replication protein A subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047192">
        <property type="entry name" value="Euk_RPA1_DBD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013955">
        <property type="entry name" value="Rep_factor-A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007199">
        <property type="entry name" value="Rep_factor-A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031657">
        <property type="entry name" value="REPA_OB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004591">
        <property type="entry name" value="Rfa1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00617">
        <property type="entry name" value="rpa1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47165:SF4">
        <property type="entry name" value="OS03G0429900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04057">
        <property type="entry name" value="Rep-A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08646">
        <property type="entry name" value="Rep_fac-A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16900">
        <property type="entry name" value="REPA_OB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Replication factor-A protein 1 N-terminal" evidence="12">
        <location>
            <begin position="5"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="domain" description="OB" evidence="11">
        <location>
            <begin position="197"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="domain" description="Replication protein A OB" evidence="14">
        <location>
            <begin position="306"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="Replication factor A C-terminal" evidence="13">
        <location>
            <begin position="463"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="119"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="149"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU364130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF16900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EFP82462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="618" mass="68661" checksum="9ED385415148C703" modified="2011-01-11" version="1">MAAQLTHNAIKRMFEDQNLEIENLTVQVISIKSVGVSNVSSAPERWRIIISDGQMFITAMLATQLNHFVQENQIKKHSILRVPSYTVNVIGSRHVVVLLGVEVVQSDYPERIGNPVNCDAEANANKDNSNTKPAPAVLANRSNVAPPKPDVKPDIDVKPKPSSYKPNIPASKPNKPGVRRTGNHIPIAGVSPFANNWKIKARVSQKTEIKRWSNSRGDGKLFSVTFLDDSGQIRATGFNDAVDNLYDSLQEGQVYLISRGKITIAKKPYNTTGHDYEIVFENTTEVEECGDSADTPKIILNKLTKLSELNDVEKDAVIDVVAVLKDVGEISEIVSKATQKTIVKRDITLIDQSAYSIRMTLWGKTAETFEAPTESIVAFQGVKVGDFGGRNLSMISSSVMLVNPDIPEAFDLKGWYDNEGVNAKIQSFANTGTGIGREITEDSLKTVAEIKDTQLGMNERGDYFNFRATIMYIKSETISYPACPTERCNKKLLRDGDDEWRCEKCDKLFPAPDHRYLIQMTVQDHTGTLWLSGFNEVGQIILPMNANELIGIKETDEAQYQKIVTDATAKTYTMVCRAKEETYNDVNRTKYSVLRIAPVDWVAAGLQLAETLLKNYSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3KE56</accession>
    <name>E3KE56_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA repair metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_08794</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA repair metallo-beta-lactamase (DRMBL) family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82598.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327017.2">
        <property type="nucleotide sequence ID" value="XM_003326969.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KE56"/>
    <dbReference type="FunCoup" id="E3KE56">
        <property type="interactions" value="361"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KE56"/>
    <dbReference type="GeneID" id="10529981"/>
    <dbReference type="KEGG" id="pgr:PGTG_08794"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08794"/>
    <dbReference type="HOGENOM" id="CLU_005260_4_1_1"/>
    <dbReference type="InParanoid" id="E3KE56"/>
    <dbReference type="OrthoDB" id="262529at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006303">
        <property type="term" value="P:double-strand break repair via nonhomologous end joining"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036297">
        <property type="term" value="P:interstrand cross-link repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16273">
        <property type="entry name" value="SNM1A-1C-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000010">
        <property type="entry name" value="DNA cross-link repair 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12650">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011084">
        <property type="entry name" value="DRMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240:SF6">
        <property type="entry name" value="DNA CROSS-LINK REPAIR 1A PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23240">
        <property type="entry name" value="DNA CROSS-LINK REPAIR PROTEIN PSO2/SNM1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07522">
        <property type="entry name" value="DRMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA repair metallo-beta-lactamase" evidence="7">
        <location>
            <begin position="560"/>
            <end position="717"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="188"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="456"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="749"/>
            <end position="775"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="20"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="47"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="113"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="235"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="457"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="766"/>
            <end position="775"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF07522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP82598.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="797" mass="88519" checksum="1F19B8C3609BDEAB" modified="2012-04-18" version="2">MQNKKKTAPSKQQKRVSARTAETSTQRSIDSFFSPTTTEPKAKKKSTTRDDPHEEKEKKKKPTKKRDSTEHPSASSSATKKRNKNNKQPVSSSDHTRPILIEDDQEETPSLEEPQKAKEGAKPDMVESGMTCPVCSKLLDCQGNVSLAERHINLCLDQPGPSVPKPPQSPTSIMQDQLDVPDTIGFEEAEEDEEEGSVKSLLSETRSDQAPGPSSAKPRATKRKTRVTKQSARKSTVEKTEGYRKGGAGKKAVPFYKLMPGTTISVDCFSHGPVPGVSAYFLSHAHSDHYTKLSSSWKHGKIYCSKTTANLVKLKLRVDSHWIVPLDFNTPYTVDDVRVVLIDANHCPGSAMFLFEGITKPDRKPFRYLHCGDFRATPAQLRHPAIHEKVIDICYLDTTYLDPKYSFPAQDQVIQGCCKAIGARVLDDDHTATMSSKDIKTLQDIGRSRAVLKSWLQPHSASSSSTTLGPLPKKEGVPEQVAGASGEEPKQPKLASIFEPKDQTSTNEKRKSLILIGTYSIGKERIVIEVAKKLGSKVFCADARKTGIIGAIDRADEEAELQGLLSADPLRAQIHLVNLFALNKPGFLEAYLHKFRPRFNHIIGIKPTGWCYKPSTPSSIALNSIDFSAFIETFQKQQQQPLQDDDEVVVEGERRGNNSRRNNPVGQLIFPEKVKPELAGLVEVYGVPYSEHSSFFELSCFCISFDWVRMIPTVNCGSPGSRAKMKGWIDRWKIERARRLLLLNNSSSALQKTRQSPETLPPPPHSSKLPSPSTTSSELVLHASAPLLIKPRDQHYW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3KEA4</accession>
    <name>E3KEA4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_08833</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82637.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327056.2">
        <property type="nucleotide sequence ID" value="XM_003327008.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KEA4"/>
    <dbReference type="STRING" id="418459.E3KEA4"/>
    <dbReference type="GeneID" id="10530020"/>
    <dbReference type="KEGG" id="pgr:PGTG_08833"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08833"/>
    <dbReference type="HOGENOM" id="CLU_858257_0_0_1"/>
    <dbReference type="InParanoid" id="E3KEA4"/>
    <dbReference type="OrthoDB" id="10348482at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24383:SF20">
        <property type="entry name" value="C2H2-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24383">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="8" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP82637.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="324" mass="36859" checksum="32C72399D5DEE074" modified="2012-04-18" version="2">MDIINTLEFSKHLSKQAQQAETPSNEPNLSDLQLHPHELEAIQVLAKLINNGTTYWSSPDMDLGLSMIKPLAVLFLHPSTDKFQNFLALESLSYLSGSEDDTAEILRWSQILDQLDGIIESEPSKQAIHFMDSLLCDPAYRKRFAPPEDSTQIDTAYMTRLKNFILLIKEEDHHVHCAAASALWNLTHSSPEVCKLILSDPSLTETLLKSFRQWSGGERDVAGFYLSTSGSMIGVFTNIYKQLDCSPEDNPPALFVDDRLHYALWLIDDYCGEGMEHGEFKDCRDLQDDVTELSTWIVDNFEARKKFNHFDLNGRESADYDAYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3KEY4</accession>
    <name>E3KEY4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09997</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178284">
        <property type="protein sequence ID" value="EFP83029.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327448.2">
        <property type="nucleotide sequence ID" value="XM_003327400.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KEY4"/>
    <dbReference type="GeneID" id="10544741"/>
    <dbReference type="KEGG" id="pgr:PGTG_09997"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09997"/>
    <dbReference type="eggNOG" id="ENOG502QS59">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_389388_0_0_1"/>
    <dbReference type="InParanoid" id="E3KEY4"/>
    <dbReference type="OrthoDB" id="1708389at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021709">
        <property type="entry name" value="DUF3292"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38694">
        <property type="entry name" value="CONSERVED EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38694:SF1">
        <property type="entry name" value="PEROXIN DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11696">
        <property type="entry name" value="DUF3292"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="395"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="511"/>
            <end position="533"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="547"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="434"/>
            <end position="454"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="513"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="552"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83029.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="709" mass="78571" checksum="93113824F41A868F" modified="2012-04-18" version="2">MESDPLFPPDFPTHLRRFTRHITAVQTRGINNSSTDLLFTSLASDQPPPPASYVPAHDSYDLQLHPVRSFRPNLLIDNVERLFISVVPRWSSGINEVKRLTTWRNPNRTAVWATVYSLCWWHEMVLPAALAFIICLSVWPKYAGNILFPPETTDPLFTGTDGARNPENLEHADPSSDEVAEVAAAATAKFIHDADAKSPLDTPSAEEFNKTPGNGPVDMLDKPSSSSSKPKEPKIGKKNLSQQYKPVIYKYGGGIQNIAGDLCDIHERLSNLFNRRFVPPPSRLRHISAAHNQSFEDPDPGMDANVRFALPFIPILFFSLFLSTHTLGRCVTFAIGFAFFLLDPIQTRSKLVRDALDANQTILRGVPTDRAFVLATLRNMQQRHDEGLDIAMKLLKKPKSPELPPRKRASDEEIDSDGVSTLSSNHASGSNPRSLFSTSKSGSSQFSRSSSSSSAPAERHQTLMKFAKKITNVAERGTQLLNEATAQSSQIINGDRQSGLDFSLLLPNKSRKVSSSSDAGSVKSSSRNQSKTNKLLRAINDTCSTHLHSAPGSSIDEGDSLSSSNSPFDPERNSSSFFCYYGNMPGHLTIHFSRTPVSRPNLSFYSGLGQSFKVRKSLFVIPLSQIVAMRKVSMLSLAGVWGGIDGIELYEKRFRDHSEESDESSDSNPSNIKLKHHTFRNMTGRDEAFLKLLVLKDWDENEEPGWISV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3KF34</accession>
    <name>E3KF34_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GTP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_09833</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">GTPase involved in activation of the TORC1 signaling pathway, which promotes growth and represses autophagy in nutrient-rich conditions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the GSE complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the GTR/RAG GTP-binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178284">
        <property type="protein sequence ID" value="EFP82865.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327284.2">
        <property type="nucleotide sequence ID" value="XM_003327236.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KF34"/>
    <dbReference type="STRING" id="418459.E3KF34"/>
    <dbReference type="GeneID" id="10544575"/>
    <dbReference type="KEGG" id="pgr:PGTG_09833"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09833"/>
    <dbReference type="HOGENOM" id="CLU_2172289_0_0_1"/>
    <dbReference type="InParanoid" id="E3KF34"/>
    <dbReference type="OrthoDB" id="10020193at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990131">
        <property type="term" value="C:Gtr1-Gtr2 GTPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006762">
        <property type="entry name" value="Gtr1_RagA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259">
        <property type="entry name" value="RAS-RELATED GTP BINDING RAG/GTR YEAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259:SF1">
        <property type="entry name" value="RAS-RELATED GTP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04670">
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP82865.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="110" mass="12504" checksum="6918370A3402C8D7" modified="2012-04-18" version="2">MRNMIFANMAAADTRKLGATIDMETTPIKFLGHFAIRLVDCGGQDSYMHNVLSMPARNRSSVFSQIGCLLYVFDVESPDFSTIDMIWFSRVILAILETLSESEKYGRIEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KF71</accession>
    <name>E3KF71_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_09866</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178284">
        <property type="protein sequence ID" value="EFP82898.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327317.2">
        <property type="nucleotide sequence ID" value="XM_003327269.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KF71"/>
    <dbReference type="GeneID" id="10544609"/>
    <dbReference type="KEGG" id="pgr:PGTG_09866"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09866"/>
    <dbReference type="HOGENOM" id="CLU_140509_0_0_1"/>
    <dbReference type="InParanoid" id="E3KF71"/>
    <dbReference type="OrthoDB" id="10294471at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP82898.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="133" mass="15699" checksum="D5A87BE8430942C3" modified="2012-04-18" version="2">MPFLNNYTDRPPHPRPFMTTSLANQMEDVRQTTTPSQAPSPFIQTRPQEQEHTEIVVDNADLDMWLNLFNNQFFMFARAQETSNVRDMRFILTQAAKTQDRILEIVEREETIRLRELAPERRTSSVYDRLSLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3KG66</accession>
    <name>E3KG66_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="5">2-methylcitrate dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_09115</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PrpD family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EFP83162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327581.1">
        <property type="nucleotide sequence ID" value="XM_003327533.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KG66"/>
    <dbReference type="STRING" id="418459.E3KG66"/>
    <dbReference type="GeneID" id="10544875"/>
    <dbReference type="KEGG" id="pgr:PGTG_09115"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09115"/>
    <dbReference type="eggNOG" id="ENOG502QPZI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_021803_1_0_1"/>
    <dbReference type="InParanoid" id="E3KG66"/>
    <dbReference type="OMA" id="DHSVMYI"/>
    <dbReference type="OrthoDB" id="10055203at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047547">
        <property type="term" value="F:2-methylcitrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019541">
        <property type="term" value="P:propionate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019679">
        <property type="term" value="P:propionate metabolic process, methylcitrate cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.120:FF:000001">
        <property type="entry name" value="2-methylcitrate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4100.10">
        <property type="entry name" value="2-methylcitrate dehydratase PrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.120">
        <property type="entry name" value="2-methylcitrate dehydratase PrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012705">
        <property type="entry name" value="2Me_IsoCit_deHydtase_PrpD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036148">
        <property type="entry name" value="MmgE/PrpD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042183">
        <property type="entry name" value="MmgE/PrpD_sf_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042188">
        <property type="entry name" value="MmgE/PrpD_sf_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005656">
        <property type="entry name" value="MmgE_PrpD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045337">
        <property type="entry name" value="MmgE_PrpD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045336">
        <property type="entry name" value="MmgE_PrpD_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006943">
        <property type="entry name" value="PRK09425.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02330">
        <property type="entry name" value="prpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16943">
        <property type="entry name" value="2-METHYLCITRATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16943:SF16">
        <property type="entry name" value="2-METHYLCITRATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19305">
        <property type="entry name" value="MmgE_PrpD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03972">
        <property type="entry name" value="MmgE_PrpD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103378">
        <property type="entry name" value="2-methylcitrate dehydratase PrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MmgE/PrpD N-terminal" evidence="3">
        <location>
            <begin position="85"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="MmgE/PrpD C-terminal" evidence="4">
        <location>
            <begin position="355"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF19305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP83162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="552" mass="61272" checksum="72A2969B23F717AA" modified="2011-01-11" version="1">MRLSPGCTRLLRHHHTQLKPILSTQLRSASTAVSRQTKIGRSQLESLSKLARPSRGFTSAAINMGGASHGAEDTTRPKPEQVLVDIAKYVHDPKFDDPALAYETARLCLIDTLGCGLEALRFPTCSNLLGPVVEGTVVPNGTRVPGTAFVLDPIRGAFNIGTMIRWLDYNDCFLAAEWGHPSDNLGSILAVADWLSRTAVANGKKPLKMHDVLTAMIQAHEIQGGLALENSFNRVGLDHVVLVKVASAAVTSKLLGLSFDQTIDVISQAFVDGQSLRTYRHAPNTGSRKSWAAGDACSRAVNLALLVQKGEMGYPTVLSAKTWGFYDVLFGGKPFKFQRPFTSYIMENVLFKISYPAEFHAQTAVECAHILHKKLVDMGKGSDDIKSVKIRTQEAAIRIIDKQGPLHNFADRDHTIQYMVAIPLIYGRLTTEDYTDKVASDPRIDELRAKISCTEEKKYSAEYHAPDKRSIGNAITIELKDGTVLDEVEIEYPVGHKRRREEGKPLLDAKFKRHIEPHFPKEQIAKIMEVVNDQKTLENFDVDKFMDLWVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>E3KG87</accession>
    <name>E3KG87_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Fe2OG dioxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_09135</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the iron/ascorbate-dependent oxidoreductase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EFP83182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327601.1">
        <property type="nucleotide sequence ID" value="XM_003327553.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KG87"/>
    <dbReference type="STRING" id="418459.E3KG87"/>
    <dbReference type="GeneID" id="10544895"/>
    <dbReference type="KEGG" id="pgr:PGTG_09135"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09135"/>
    <dbReference type="eggNOG" id="KOG0143">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010119_6_0_1"/>
    <dbReference type="InParanoid" id="E3KG87"/>
    <dbReference type="OMA" id="DESFWVM"/>
    <dbReference type="OrthoDB" id="288590at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016706">
        <property type="term" value="F:2-oxoglutarate-dependent dioxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.330">
        <property type="entry name" value="B-lactam Antibiotic, Isopenicillin N Synthase, Chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026992">
        <property type="entry name" value="DIOX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044861">
        <property type="entry name" value="IPNS-like_FE2OG_OXY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027443">
        <property type="entry name" value="IPNS-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050231">
        <property type="entry name" value="Iron_ascorbate_oxido_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005123">
        <property type="entry name" value="Oxoglu/Fe-dep_dioxygenase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47990">
        <property type="entry name" value="2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03171">
        <property type="entry name" value="2OG-FeII_Oxy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14226">
        <property type="entry name" value="DIOX_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00682">
        <property type="entry name" value="IPNSYNTHASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51471">
        <property type="entry name" value="FE2OG_OXY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe2OG dioxygenase" evidence="2">
        <location>
            <begin position="176"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP83182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="349" mass="39086" checksum="6D826BF66FFFD10B" modified="2011-01-11" version="1">MSLPMIDIAGFLEPSSTPEALQTIADKIHAACLTTGFFYLSGHGVTAEEGEQILRSTRAFLVDGTPGEKERLSIATNDHARGYQRIGDNVTGGKSDWHEALDLYAPSPFPHLPQLEQLKVLAGQNRFPARPADFQPVVENWIRRLSELGLVLMRATAMGLKMSLSETEQLLDQVRNSFWVLRCIGYPPLPSTHDGVSCGAHKDYGCFTFLHADSTKGSLQVYVPKAKATHDEQEPEYHWIEANPIEGAFVVNIGEMWETWTNGLYKATLHRVVHKSNNYRVSVPFFYEPAFDAIVAPLPAAIRLQQRLQGKQLVEIVKKPVQYGEFLISKVSRNFSTSADSDAQRRDRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGB6</accession>
    <name>E3KGB6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_08527</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327760.1">
        <property type="nucleotide sequence ID" value="XM_003327712.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KGB6"/>
    <dbReference type="GeneID" id="10545057"/>
    <dbReference type="KEGG" id="pgr:PGTG_08527"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08527"/>
    <dbReference type="HOGENOM" id="CLU_1441707_0_0_1"/>
    <dbReference type="InParanoid" id="E3KGB6"/>
    <dbReference type="OrthoDB" id="10269262at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP83341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="188" mass="21518" checksum="40D04A53C67B2D1B" modified="2011-01-11" version="1">MSEMWDVLTDPQSLRPGKLPLATRGDEVAVLSHYAMMYCLNDIWVEFLAVSAKHNLLSDGCLEGYLNNKNAAHGVYNYIMGKFVPWQSIASLYLNLDLKLGLEESQFARGARSLWKHVSPSIWQTLTRLYLVAQFRSLAYGATYQTEELLRLINPFEIVTLDEDYKRVYQPESTTHLVGRNPSRRDGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGI0</accession>
    <name>E3KGI0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rhodanese domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_08591</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83405.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327824.2">
        <property type="nucleotide sequence ID" value="XM_003327776.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KGI0"/>
    <dbReference type="FunCoup" id="E3KGI0">
        <property type="interactions" value="261"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KGI0"/>
    <dbReference type="GeneID" id="10545121"/>
    <dbReference type="KEGG" id="pgr:PGTG_08591"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08591"/>
    <dbReference type="eggNOG" id="KOG3772">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_107716_1_1_1"/>
    <dbReference type="InParanoid" id="E3KGI0"/>
    <dbReference type="OrthoDB" id="102559at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828:SF38">
        <property type="entry name" value="ARSENICAL-RESISTANCE PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828">
        <property type="entry name" value="M-PHASE INDUCER PHOSPHATASE DUAL SPECIFICITY PHOSPHATASE CDC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="2">
        <location>
            <begin position="59"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP83405.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="185" mass="20931" checksum="B69066BDF8E8FF46" modified="2012-04-18" version="2">MRPPQRETCDKPSQPSPHRVTFSRLLHKPNHSRRASQAWDFISFAPPRVIANRIKTGRLNKDWAVVDVRDSDFIGGHIPGCHHIPSENFHEKCKALIDELKDVKCVIFHCALSQQRGPTAAKLYAHRREDNLVSGSLKSLLPFGTEAQARGEAQEVMIVQGGFKEWAEEFKDDPALVEGYNPALW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGU3</accession>
    <name>E3KGU3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">M-phase inducer phosphatase</fullName>
            <ecNumber evidence="2">3.1.3.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_08704</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 14">
            <text>O-phospho-L-tyrosyl-[protein] + H2O = L-tyrosyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10684"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:20101"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:61978"/>
            <dbReference type="EC" id="3.1.3.48"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MPI phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.48" evidence="2"/>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83518.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327937.2">
        <property type="nucleotide sequence ID" value="XM_003327889.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KGU3"/>
    <dbReference type="STRING" id="418459.E3KGU3"/>
    <dbReference type="GeneID" id="10532199"/>
    <dbReference type="KEGG" id="pgr:PGTG_08704"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08704"/>
    <dbReference type="eggNOG" id="KOG3772">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_319365_0_0_1"/>
    <dbReference type="InParanoid" id="E3KGU3"/>
    <dbReference type="OrthoDB" id="26523at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004725">
        <property type="term" value="F:protein tyrosine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010971">
        <property type="term" value="P:positive regulation of G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110032">
        <property type="term" value="P:positive regulation of G2/MI transition of meiotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01530">
        <property type="entry name" value="Cdc25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.250.10:FF:000021">
        <property type="entry name" value="M-phase inducer phosphatase cdc-25.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000751">
        <property type="entry name" value="MPI_Phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828">
        <property type="entry name" value="M-PHASE INDUCER PHOSPHATASE DUAL SPECIFICITY PHOSPHATASE CDC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10828:SF17">
        <property type="entry name" value="PROTEIN-TYROSINE-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00716">
        <property type="entry name" value="MPIPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0498">Mitosis</keyword>
    <keyword evidence="6" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="11">
        <location>
            <begin position="699"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="102"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="331"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="404"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="500"/>
            <end position="527"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="593"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="229"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="241"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="500"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="601"/>
            <end position="611"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00067190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP83518.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="898" mass="98518" checksum="9D3D6A087986B733" modified="2012-04-18" version="2">MPSSHFLVSSPTPAHQLHGSFCQFEPNPSPLLRSSIYRRKKTNHAPQYDHDGNYHNSSLLESKRFLEAEVPAEHDKQLPEMCNEDRYGDLVDEQDQQTFEGTYVEDEPHDEEDELEEDGDPSESAEQVIGPFSPTLDVSFASSMSNGTAPPTPSPYPQGSRFTHNAQLAPVSSKHSFPPAHLIAPNPNVMPHSSSPHRMDISPAPISTRCNIPSYPHQPNSFRPHSPDDPFGGSSSGSPSHTLSKLSPTTRNTRRTVGLRKTQSRPNLSSEALFLHPSRTMDEKSSPCALARTSDCMDIDSPSYAQAHHCHSLHSQMGSDDMPSGARCLCHRTSRSTSQSSTGKRSRSDEDEEREQSPSRPSSSGCNRQSADKAHVSSPSELSLAGPSLADRLFGASSRLYGEPSRSFSPFEPSSSPSDTMSPSVKAAAKITVGRKASERPLSGFRFPASKSYIPSAIIPSTTRPQHLGPANVGLAFGNRTTHRRPVLGVVQSSATLFSRHQSTSTTSAGMDRPRDMSGRPASKSRRAISFAATEGDSHNLMLQCDGMVADPDEDEEESELDVSLDFNCSPAARTSKKASHSSLVSSIWPKYTESPIRPNRSASHNDSRLNPNRKPSGAGASNSQSVLPTFELAMASQSPTRTFAATLTSTIESPVGMAFSEKERAGKILPCHKVSDDGLMRISPDTMDRLLEGLYDQNISQTIVIDCRFGYEYEGGHIRSAINIQDKGLVDKMLLQGGIFTRGKGDVPLPSESGKIDSTGQNKKVVLVFHCEYSAMRAPTTAKYLREQDRHMNMSHYPALHYPEVYILEGGYARYFAHSPQHCEGGYVGMDDPSHRSDRHADLNQFRTRESAFSSRAKSFTYGESQKSMKNGNHQKKPVFLFDQACSESNKPRVIFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KI91</accession>
    <name>E3KI91_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09729</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EFP84016.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328435.2">
        <property type="nucleotide sequence ID" value="XM_003328387.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KI91"/>
    <dbReference type="GeneID" id="10532711"/>
    <dbReference type="KEGG" id="pgr:PGTG_09729"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09729"/>
    <dbReference type="HOGENOM" id="CLU_1918136_0_0_1"/>
    <dbReference type="InParanoid" id="E3KI91"/>
    <dbReference type="OrthoDB" id="2495615at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172020" evidence="1">
        <location>
            <begin position="22"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84016.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="133" mass="14793" checksum="F379791CBA835047" modified="2012-04-18" version="2">MRFPSLAMMGWMALACGLVIAHKHSEKKTKVVFSCLDDPDEDSQYSQAACGRAIELEDSQNPSSSPTKYSFVKANQVRKSKTEYNCIDTNMDNNYCCFADTFKFKNKSVDVDAQTVEENCIVVPGNTDSKPNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJ20</accession>
    <name>E3KJ20_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ubiquitin carboxyl-terminal hydrolase</fullName>
            <ecNumber evidence="7">3.4.19.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_10673</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).</text>
            <dbReference type="EC" id="3.4.19.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the peptidase C19 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.19.12" evidence="7"/>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328714.1">
        <property type="nucleotide sequence ID" value="XM_003328666.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJ20"/>
    <dbReference type="FunCoup" id="E3KJ20">
        <property type="interactions" value="147"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KJ20"/>
    <dbReference type="GeneID" id="10545639"/>
    <dbReference type="KEGG" id="pgr:PGTG_10673"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10673"/>
    <dbReference type="eggNOG" id="KOG1865">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_399078_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJ20"/>
    <dbReference type="OMA" id="HVEYERS"/>
    <dbReference type="OrthoDB" id="420187at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016579">
        <property type="term" value="P:protein deubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031647">
        <property type="term" value="P:regulation of protein stability"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.10:FF:000248">
        <property type="entry name" value="Ubiquitin carboxyl-terminal hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.10">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050164">
        <property type="entry name" value="Peptidase_C19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001394">
        <property type="entry name" value="Peptidase_C19_UCH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028889">
        <property type="entry name" value="USP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018200">
        <property type="entry name" value="USP_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24006:SF758">
        <property type="entry name" value="UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00443">
        <property type="entry name" value="UCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00972">
        <property type="entry name" value="USP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00973">
        <property type="entry name" value="USP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50235">
        <property type="entry name" value="USP_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 7" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0788">Thiol protease</keyword>
    <keyword evidence="4 7" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="USP" evidence="9">
        <location>
            <begin position="180"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="20"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="99"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="502"/>
            <end position="690"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="52"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="113"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="151"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="505"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="541"/>
            <end position="553"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="585"/>
            <end position="595"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="615"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="653"/>
            <end position="669"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP84295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="690" mass="76501" checksum="09661638F1A3BCF2" modified="2011-01-11" version="1">MAAVASSTHLHLHLNHLIINHDKQPPTNNNNNSNKLSSTSKHHPEPTQATDQANTRTTTNPRTQPSQQQLGKLWEQALQQPIKFIKSSNQLNLTQIPYQPINQPQPATQPTRPSNSANTNPTTTSNQSNLTSSSASSLSEKISPNSNPITQSTSSRLSQSNSSASSQVATYWIRPQPIGPGFYNTGNTCFLNATLQALVHTPALAIGLMDRDEHSPESCDLARAKKFCALCRMYRLVNMCFDKASYGSHAIEPSPINRALSKFAPTMRGGRQEDAHEFLRLLVEAMQNGALQGRAEKAKQKQKESTFVHRMFGGKLRSRVVCEHCNTPSDTFDNFLDLSLDISQANSVSSALKAYHKFDRLRGANQYKCDKCKCLRDAKKSMSVFSAPPILTLHLKRFNFRGRKINKRVNFEDSLDLKPAMSNESDITGYKLYAVVCHRGQSNKSGHYYAHVKASNGKWYVADDSTVESISDSRTVLANEHAYILFYARDRESLLSAAIAGNRQSTSTTTNSDGGNRGSFTKRKPSISDDRHSQVNGVAEKTGKHSNRTENGHKRVRTGSINSQESPAKSFIGPVIPRQSLPSFKFKDTHQDGSDSTRGSPAKAKQNPEQDAHQSGRSSLTRSPSSHSNSNHNSNDQQDQLSDDQVEQPMNQMKKKKRNRKPKSKRRKIVNIAGTPVNQVPSFKPRLITG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJK2</accession>
    <name>E3KJK2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_10197</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178290">
        <property type="protein sequence ID" value="EFP84477.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328896.2">
        <property type="nucleotide sequence ID" value="XM_003328848.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJK2"/>
    <dbReference type="GeneID" id="10545987"/>
    <dbReference type="KEGG" id="pgr:PGTG_10197"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10197"/>
    <dbReference type="HOGENOM" id="CLU_2688989_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJK2"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP84477.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="74" mass="8083" checksum="5085F264A86D4724" modified="2012-04-18" version="2">MEYSMTQEERFLTATKATIRIVARGSKALRSKVSLEIVRAQSIMDHSDLSSKAPFHISKALLVPTSALVPTVPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KKE3</accession>
    <name>E3KKE3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10927</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84768.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329187.2">
        <property type="nucleotide sequence ID" value="XM_003329139.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KKE3"/>
    <dbReference type="GeneID" id="10526737"/>
    <dbReference type="KEGG" id="pgr:PGTG_10927"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10927"/>
    <dbReference type="HOGENOM" id="CLU_132787_0_0_1"/>
    <dbReference type="InParanoid" id="E3KKE3"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="26"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="116"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84768.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="127" mass="13894" checksum="8BBF57E1B7A644F0" modified="2012-04-18" version="2">MVSISSDSRLQVAHPTQKWPAVAELSTDSATDARTTLDSDSDGESAPPKFKSASSKSRPKKKKPRQQIVVSKKNKATVSVAAKQKATLTSEDYQAHDYNQDTDVGSIEIQPQGKKNSKEDEYAKIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3KKX8</accession>
    <name>E3KKX8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_10424</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178292">
        <property type="protein sequence ID" value="EFP84953.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329372.2">
        <property type="nucleotide sequence ID" value="XM_003329324.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KKX8"/>
    <dbReference type="STRING" id="418459.E3KKX8"/>
    <dbReference type="GeneID" id="10526928"/>
    <dbReference type="KEGG" id="pgr:PGTG_10424"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10424"/>
    <dbReference type="HOGENOM" id="CLU_1982655_0_0_1"/>
    <dbReference type="InParanoid" id="E3KKX8"/>
    <dbReference type="OrthoDB" id="2507178at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023:SF4">
        <property type="entry name" value="GLYCINE-RICH PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="2">
        <location>
            <begin position="68"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="102"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP84953.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="126" mass="14121" checksum="CECEBA540ED9955B" modified="2012-04-18" version="2">MCCLVKHYSGRHCWERSEGYDLLVTNPRKSYGTHRCYSNAKRWLKSGKTRNDILTEAKALYITASGSPFNLDHCWGILKDTPKWQATQRENDARGKKAQVASTTSLSTDLPSSSIQVSSPAVIDID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3KKY2</accession>
    <name>E3KKY2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10428</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178292">
        <property type="protein sequence ID" value="EFP84957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329376.1">
        <property type="nucleotide sequence ID" value="XM_003329328.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KKY2"/>
    <dbReference type="FunCoup" id="E3KKY2">
        <property type="interactions" value="13"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KKY2"/>
    <dbReference type="GeneID" id="10526932"/>
    <dbReference type="KEGG" id="pgr:PGTG_10428"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10428"/>
    <dbReference type="eggNOG" id="ENOG502QSKI">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019711_0_0_1"/>
    <dbReference type="InParanoid" id="E3KKY2"/>
    <dbReference type="OMA" id="IEAYQHC"/>
    <dbReference type="OrthoDB" id="434695at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034044">
        <property type="term" value="C:exomer complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043001">
        <property type="term" value="P:Golgi to plasma membrane protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006893">
        <property type="term" value="P:Golgi to plasma membrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006896">
        <property type="term" value="P:Golgi to vacuole transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000146">
        <property type="entry name" value="Clathrin-coated vesiclec protein (Bud7)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000149">
        <property type="entry name" value="Clathrin-coated vesiclec protein (Bud7)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015374">
        <property type="entry name" value="ChAPs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31975">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31975:SF1">
        <property type="entry name" value="BUD SITE SELECTION PROTEIN 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09295">
        <property type="entry name" value="ChAPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="466"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="569"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="763" mass="85450" checksum="8456131E5E9360E4" modified="2011-01-11" version="1">MSDHLKDVPEFFEVELGESLISRTEFLTSFRELGPPDLCHLIKTTSKPGSKPPTTTNGVERDLGSYHYVSGADASSSASLAAYLNSLTYSIEESGSWLGGWGGGGGKGAWRIRSGCYCCFNAFSRVDVRVEVKIPGGVDAYLIDLRGEKHEPTPVIWTETYVSAILRAILYADDPNYRLAGFRKIDPITNPEGEQRFLRAVQEIFFKGWQLGSDPEIQVATVISNHLTTGIMKYFGDGFRWGPAVNLFEKLAVKEVEVAALLAQAYLGMNEEVKAVQVLHRALEDMPQSYALLHVQCDFLRSKGGQYTEWALKLARQAVNCAPSEFITWAKLTEVYIELGRYSDALLTLNSCPMFTYNDRDLHRMPTPSRSHLPIKTFIAESQILDEDSARDNEADIALLRLPAPSLRGTFSKAYSLLTKLVILIGWDELLKCRSQVFVMEEEYRQQRTETEQQGSFQSHAKRFSTSAMGNGEGKAATDDAPNASSRKEPSPDPQASAAYNPPSPIPTITVSDSEDHRPSEARNPVDVSSKSDDESRPDSPVGLDEASRSPMAGPASGVDKPIAAHPSSGITDKQSENTASTATAAPRNTFMNKRLCERWLDNLFMVLYEDLRVYTIWRAEVAHFKAQHMPYRKTGTEWEILGDLAFRLHHKEEAKDAFQRCLEAKFSAKAWMKLLEFYTDEGDVQRSLNAAIRLSTYQHRWYMEMSFPTAVAHQLYKLMRQEGVAKISYSLVSMNLPQPIVKTCMQGYFNYAQVFRIEGSDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3KL88</accession>
    <name>E3KL88_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cytochrome c oxidase subunit XVII assembly protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_11232</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion intermembrane space</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COX17 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP85063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329482.1">
        <property type="nucleotide sequence ID" value="XM_003329434.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KL88"/>
    <dbReference type="FunCoup" id="E3KL88">
        <property type="interactions" value="135"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KL88"/>
    <dbReference type="GeneID" id="10527040"/>
    <dbReference type="KEGG" id="pgr:PGTG_11232"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11232"/>
    <dbReference type="eggNOG" id="KOG3496">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_149618_1_2_1"/>
    <dbReference type="InParanoid" id="E3KL88"/>
    <dbReference type="OMA" id="QAHLTCM"/>
    <dbReference type="OrthoDB" id="1915887at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016531">
        <property type="term" value="F:copper chaperone activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033617">
        <property type="term" value="P:mitochondrial respiratory chain complex IV assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1130:FF:000005">
        <property type="entry name" value="Cytochrome c oxidase assembly protein subunit 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1130">
        <property type="entry name" value="CytochromE C oxidase copper chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009069">
        <property type="entry name" value="Cys_alpha_HP_mot_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007745">
        <property type="entry name" value="Cyt_c_oxidase_Cu-chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16719">
        <property type="entry name" value="CYTOCHROME C OXIDASE COPPER CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16719:SF0">
        <property type="entry name" value="CYTOCHROME C OXIDASE COPPER CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05051">
        <property type="entry name" value="COX17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47072">
        <property type="entry name" value="Cysteine alpha-hairpin motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 8" id="KW-0186">Copper</keyword>
    <keyword evidence="6" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR607745-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP85063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="68" mass="7628" checksum="14A57F0405C554F1" modified="2011-01-11" version="1">MEAAQAQKVQTTKPEKIKPCCACPETKRVRDDCFLRFGPPDESKESTIKCAELIAAHRACMAQYGFHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLI8</accession>
    <name>E3KLI8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Eukaryotic translation initiation factor 1A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Eukaryotic translation initiation factor 4C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_11332</name>
        <name evidence="13" type="ORF">PGTG_19886</name>
    </gene>
    <organism evidence="12 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <reference evidence="12" key="3">
        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 9">Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the eIF-1A family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP85163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="DS178421">
        <property type="protein sequence ID" value="EFP93956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329582.1">
        <property type="nucleotide sequence ID" value="XM_003329534.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338375.1">
        <property type="nucleotide sequence ID" value="XM_003338327.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLI8"/>
    <dbReference type="FunCoup" id="E3KLI8">
        <property type="interactions" value="599"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KLI8"/>
    <dbReference type="GeneID" id="10527144"/>
    <dbReference type="GeneID" id="10535569"/>
    <dbReference type="KEGG" id="pgr:PGTG_11332"/>
    <dbReference type="KEGG" id="pgr:PGTG_19886"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11332"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19886"/>
    <dbReference type="HOGENOM" id="CLU_109098_0_1_1"/>
    <dbReference type="InParanoid" id="E3KLI8"/>
    <dbReference type="OMA" id="RRVMMHA"/>
    <dbReference type="OrthoDB" id="274995at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006413">
        <property type="term" value="P:translational initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05793">
        <property type="entry name" value="S1_IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000071">
        <property type="entry name" value="Eukaryotic translation initiation factor 1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00216">
        <property type="entry name" value="aIF_1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001253">
        <property type="entry name" value="TIF_eIF-1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018104">
        <property type="entry name" value="TIF_eIF-1A_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00523">
        <property type="entry name" value="eIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21668">
        <property type="entry name" value="EIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00652">
        <property type="entry name" value="eIF1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01262">
        <property type="entry name" value="IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7 9" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 7 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1-like" evidence="11">
        <location>
            <begin position="22"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00032507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00067293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP85163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP93956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="141" mass="16236" checksum="B75E48EC8E5C1977" modified="2011-01-11" version="1">MPKNKGKGGKNRRRGKNENECEKRELVFKEDGQEYAQVTKMLGNGRLEAMCFDGEKRLAHIRGKMRKKVWINQGDIILISLRDFQDDKADVIQKYTTDEARNLKAYGELPESAKINETDTFGPGEDDECNFEFEEADIDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLL3</accession>
    <name>E3KLL3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11357</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP85188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329607.1">
        <property type="nucleotide sequence ID" value="XM_003329559.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLL3"/>
    <dbReference type="GeneID" id="10527171"/>
    <dbReference type="KEGG" id="pgr:PGTG_11357"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11357"/>
    <dbReference type="HOGENOM" id="CLU_043422_0_0_1"/>
    <dbReference type="InParanoid" id="E3KLL3"/>
    <dbReference type="OrthoDB" id="2499834at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00084">
        <property type="entry name" value="HMG-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="474" mass="52652" checksum="98DC3F9A1D0249BF" modified="2011-01-11" version="1">MPPCSVVHILPPQYQDNFNRAILDTRSPIPSLNQHAAILQTGTTAPIIDHLENSSSQTPQLDVQVPAPPPSNSLTQPPSKKRKHTVESNESMLPQSIDVLLKKSYNELVTHAQENSKGSMSQADRDFFLEFYEEQRKLLAIKAIEHQLSMPMVDAFLGKRLALKGPNCWNQFLKTPEARAIFKETGGVDDQSAMKKVSELWHKLTPEEKKGFANKIDDGLTDEERALDDDNSEELIVRVEPAAPSTSGKDSVPTSGQLSVRTEGSFRHDYLAVQNFVNDFVAKATHISATHNSQFVVFAVSTHLGPSSYQISQCTPGANVFLTYAKDVDAEHHYAARFQSHITGYTVAQIAELADKTPQKNVKKPSHEVKVTARMTALIKAKTEGVVKRWPWTDTVSRLAEIGFEIVLAPSARINFDWITQPSRQLKVEHIDLLHLDLDDQLIDILRIPRNLAGGTRRAPSLEGVAPQTMPLVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNJ6</accession>
    <name>E3KNJ6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_11627</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178297">
        <property type="protein sequence ID" value="EFP85871.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330290.2">
        <property type="nucleotide sequence ID" value="XM_003330242.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNJ6"/>
    <dbReference type="GeneID" id="10544453"/>
    <dbReference type="KEGG" id="pgr:PGTG_11627"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11627"/>
    <dbReference type="HOGENOM" id="CLU_2639273_0_0_1"/>
    <dbReference type="InParanoid" id="E3KNJ6"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP85871.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="77" mass="9081" checksum="0027AC6372465B85" modified="2012-04-18" version="2">MPRAADHPVNGHWLEYTGHWLELSSHRLEYTSHWLEYTGHRAEYTLQSMAGKRLVNGVSSVRQSSRPVIWWSLYSNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPL4</accession>
    <name>E3KPL4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CAMK/CAMKL protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_12205</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178299">
        <property type="protein sequence ID" value="EFP86249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330668.1">
        <property type="nucleotide sequence ID" value="XM_003330620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KPL4"/>
    <dbReference type="STRING" id="418459.E3KPL4"/>
    <dbReference type="GeneID" id="10540260"/>
    <dbReference type="KEGG" id="pgr:PGTG_12205"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12205"/>
    <dbReference type="eggNOG" id="KOG0583">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1171097_0_0_1"/>
    <dbReference type="InParanoid" id="E3KPL4"/>
    <dbReference type="OrthoDB" id="504170at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF110">
        <property type="entry name" value="NON-SPECIFIC SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP86249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="267" mass="29495" checksum="1304D4C0A435564C" modified="2011-01-11" version="1">MAPLAGGAPGRLRRHCQCVFKKKIGIVDFMKEKIKLIPKSSSPPLLTCEIHHHRWLHHPNVVQLYDVIASEHLIWLITELCSGGELFDYLVKKTWFTKFEACRLFGQICLGLGYVHGKGVVHRDLKLENVLLDEGCNPKMADFGGPHEQIAQRLRALKGSLKKLGLKVRALPPFAEKGRTRVRGETGIRTRIRIRQRVSPRLSGYPPALAGILGYSPFKGPARDRLEGFPSSQQGECLTAGRISFQPASSQLAGGQETLPAGPSPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPN0</accession>
    <name>E3KPN0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12221</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178299">
        <property type="protein sequence ID" value="EFP86265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330684.1">
        <property type="nucleotide sequence ID" value="XM_003330636.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KPN0"/>
    <dbReference type="GeneID" id="10540276"/>
    <dbReference type="KEGG" id="pgr:PGTG_12221"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12221"/>
    <dbReference type="HOGENOM" id="CLU_1636241_0_0_1"/>
    <dbReference type="InParanoid" id="E3KPN0"/>
    <dbReference type="OMA" id="CDSFKTS"/>
    <dbReference type="OrthoDB" id="2507345at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="153"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="163" mass="18037" checksum="DB71A98CC3323F77" modified="2011-01-11" version="1">MQVEVLDNTDSSSSFNKSEDNSNSKPLWRAPETTGDVTFQSLHRGSLDRSSSVTASLKESTSKGKLSMSTNTISGAQAAQMISSETSNPLSIQPRPPLSASSSNLVKRIVENQNLLIWQAYDDWRQKFPRLQFDDKDEEIEENEVESEAETEPFTSDSAADES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQ60</accession>
    <name>E3KQ60_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_12391</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178300">
        <property type="protein sequence ID" value="EFP86435.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330854.2">
        <property type="nucleotide sequence ID" value="XM_003330806.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQ60"/>
    <dbReference type="GeneID" id="10540450"/>
    <dbReference type="KEGG" id="pgr:PGTG_12391"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12391"/>
    <dbReference type="HOGENOM" id="CLU_1595360_0_0_1"/>
    <dbReference type="InParanoid" id="E3KQ60"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP86435.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="181" mass="20510" checksum="FC14F6742B5E665F" modified="2012-04-18" version="2">MSFSRPLSITSLDATITGTCSESDSIDERSPIVFDSVDICLPVDYNSVDKRSSAQPGDYSNFSPAFTRDTFLLEELHDFRILITDELIRFARSASAANKADRNQRLRLYSRVEDLFSTLHMHQLGPPMELRRALIQAKGKEEKIKDKEEMVVFHKKVEAQSGEVTEEHVSEIKALVENQGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3KRZ7</accession>
    <name>E3KRZ7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13291</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EFP87072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331491.1">
        <property type="nucleotide sequence ID" value="XM_003331443.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KRZ7"/>
    <dbReference type="GeneID" id="10542153"/>
    <dbReference type="KEGG" id="pgr:PGTG_13291"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13291"/>
    <dbReference type="HOGENOM" id="CLU_1797431_0_0_1"/>
    <dbReference type="InParanoid" id="E3KRZ7"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="144" mass="15312" checksum="9101F4A9ED100938" modified="2011-01-11" version="1">MALPGVDKRTPSPTPHSLPHPALPPPPRTPTPTPLQKGRAIEPAPPGGAGGLACQPRTKIAWFLDGFESSPSGGFPPDELFQRFCWLIEALSSIKSTVSYLAKITNLSSPEEGLVHKSGEAGVWLRGLFKAEAAPDPMTKRPSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3KS05</accession>
    <name>E3KS05_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF6818 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_13299</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EFP87080.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331499.2">
        <property type="nucleotide sequence ID" value="XM_003331451.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KS05"/>
    <dbReference type="GeneID" id="10542322"/>
    <dbReference type="KEGG" id="pgr:PGTG_13299"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13299"/>
    <dbReference type="eggNOG" id="ENOG502SF3D">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_739952_0_0_1"/>
    <dbReference type="InParanoid" id="E3KS05"/>
    <dbReference type="OrthoDB" id="99432at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049203">
        <property type="entry name" value="DUF6818"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34409:SF1">
        <property type="entry name" value="MYB-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34409">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20681">
        <property type="entry name" value="DUF6818"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6818" evidence="3">
        <location>
            <begin position="89"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="184"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="309"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="185"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="199"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="211"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP87080.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="391" mass="42831" checksum="7957426415E19BA1" modified="2012-04-18" version="2">MQLTQPSDLSQQSLPSQLINPTQPTQPTQQSDTNQFPTQSGTPASIQSNPSVRSAAATASKRPGRQKGSFGYTTPDCLALVNAVKMYLPLGSQEWGYVQVEFNKYAQESGRPTREADALKLKFRALANQTKPTGDADCPSYVCEAKATQVSIDRRAHVISCNDTENTNNGNDIEGGVDGLMQENELPESDLDGNEDTLPDPLDLTSPPSTNPAEQSRSDNSGPNSRCFSAWNQRLSTGGSGSGSHQNPLVNRPDTRVRSDGPYRGAAQSRRPGQPPAELENNMISFFDPQAREVRDHERSISQFYAVALQDSRAANVRLEAEIRELRDGDKGQANQLRIENDRLKEKLTAQQFEIQGLKNEIKFLQLRIDFAGNTQSRPSQPPNTSLDTGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KSI9</accession>
    <name>E3KSI9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12859</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178305">
        <property type="protein sequence ID" value="EFP87275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331694.1">
        <property type="nucleotide sequence ID" value="XM_003331646.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KSI9"/>
    <dbReference type="GeneID" id="10537674"/>
    <dbReference type="KEGG" id="pgr:PGTG_12859"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12859"/>
    <dbReference type="HOGENOM" id="CLU_627205_0_0_1"/>
    <dbReference type="InParanoid" id="E3KSI9"/>
    <dbReference type="OrthoDB" id="2502482at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="109"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="437" mass="48680" checksum="3582C9D3A2AD30A7" modified="2011-01-11" version="1">MSLEDFDDFSPERQLLHSERPEDDFCAILSSTKRKVGPEVMSEQQRDRLKPSCSTEQTEEDAVDGLLADLQRSHTPTRVPSSQASSDESSLEPLSEAFEVPTPLSPPLPRRELPSQRDSRRHSHTFSKPVLESELFQRLRPNSQMVTPHHSDRSHKRAKSETPSTVNSPTEERKHWTTEDDGFPGHFSHFHLEGPCRPHLPVSVPPSPMRAPINLSSPLIADVNVAKSRVLVTKTHCFYPEKSSLIPFSSCLLGFQLVFHSSDPKARIKHSQIKLTILSSEGSPSGDTPVIKAIYPSQGTIHKTGDKTLVRRTDENSIGVKLGVDPYGAMILSHTQSRTQERYTAPYLLGSGIETNKLLITMNEDSSLHAGVPPSLAFAILLYLPSDSTKAFQADLTIETSVRNEIGASIRKMWASPKVWRLNYDGKTELGCLKMHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="82" xmlns="https://uniprot.org/uniprot">
    <accession>E3KT69</accession>
    <name>E3KT69_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Kinesin-like protein Klp8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGTG_13865</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178307">
        <property type="protein sequence ID" value="EFP87494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331913.1">
        <property type="nucleotide sequence ID" value="XM_003331865.2"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KT69"/>
    <dbReference type="GeneID" id="10538397"/>
    <dbReference type="KEGG" id="pgr:PGTG_13865"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13865"/>
    <dbReference type="eggNOG" id="KOG0245">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001485_20_3_1"/>
    <dbReference type="InParanoid" id="E3KT69"/>
    <dbReference type="OMA" id="IDRGAKC"/>
    <dbReference type="OrthoDB" id="3176171at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005871">
        <property type="term" value="C:kinesin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003777">
        <property type="term" value="F:microtubule motor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005546">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008574">
        <property type="term" value="F:plus-end-directed microtubule motor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047496">
        <property type="term" value="P:vesicle transport along microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01365">
        <property type="entry name" value="KISc_KIF1A_KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01233">
        <property type="entry name" value="PH_KIFIA_KIFIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.200.20:FF:000001">
        <property type="entry name" value="Kinesin family member 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.850.10:FF:000047">
        <property type="entry name" value="Kinesin family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.200.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.850.10">
        <property type="entry name" value="Kinesin motor domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022164">
        <property type="entry name" value="Kinesin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022140">
        <property type="entry name" value="Kinesin-like_KIF1-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032405">
        <property type="entry name" value="Kinesin_assoc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019821">
        <property type="entry name" value="Kinesin_motor_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001752">
        <property type="entry name" value="Kinesin_motor_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036961">
        <property type="entry name" value="Kinesin_motor_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049780">
        <property type="entry name" value="PH_KIFIA_KIFIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008984">
        <property type="entry name" value="SMAD_FHA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47117">
        <property type="entry name" value="STAR-RELATED LIPID TRANSFER PROTEIN 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12473">
        <property type="entry name" value="DUF3694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00498">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12423">
        <property type="entry name" value="KIF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00225">
        <property type="entry name" value="Kinesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16183">
        <property type="entry name" value="Kinesin_assoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00380">
        <property type="entry name" value="KINESINHEAVY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00240">
        <property type="entry name" value="FHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00129">
        <property type="entry name" value="KISc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49879">
        <property type="entry name" value="SMAD/FHA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50006">
        <property type="entry name" value="FHA_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00411">
        <property type="entry name" value="KINESIN_MOTOR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50067">
        <property type="entry name" value="KINESIN_MOTOR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="4" id="KW-0493">Microtubule</keyword>
    <keyword evidence="8 10" id="KW-0505">Motor protein</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Kinesin motor" evidence="15">
        <location>
            <begin position="1"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="FHA" evidence="14">
        <location>
            <begin position="487"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="domain" description="PH" evidence="13">
        <location>
            <begin position="1523"/>
            <end position="1622"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="571"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1128"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="736"/>
            <end position="778"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="590"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1128"/>
            <end position="1140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1145"/>
            <end position="1158"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="86"/>
            <end position="93"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00283"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EFP87494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1622" mass="183725" checksum="5791560C0166DF1D" modified="2011-01-11" version="1">MEAGTHTFLDPPPTENEAGAGDQKSKSKSSATDKETKSFAFDHSYWSACPKDEPGYASQQTLYEDLGVDLLNNSFEGYNSCIFAYGQTGSGKSYSMMGYGQDRGIIPLTCSALFDRIQEKKVTDPAVCYTVEVSFMEIYNERVRDLLNPKNKGNLRVREHPSLGPYVEDLSKLAVQSYSDVETLMDEGNKARTVAATNMNETSSRSHSVFTLLLTQRRKDATTGMEGEKVSRISLVDLAGSERANSTGATGVRLKEGAQINKSLTTLGKVISALATAGSSGPGKKKKADDHVPYRDSVLTWLLKDSLGGNSKTAMIAAISPADYEETLSTLRYADQAKKIKNKAVVNEDPNAKLIRELKEELDTLRSRMSGGHSEDTYDVTLPPEKQIVKYQTKSGEIKTVTKAALQEQLEQSEKLMSEVAQTWEEKLKNTEVVQKEREQALEELGITIEKNNVGVYTPKRMPHLVNLNEDPLMSECLIYQIKPGTTTVGNTETTEPCDIRLSGRNILAQHCHFESDKETGAVELFSHPNAPTMVNGQRLSPGKPRKLRSGYRVILGDYHIFRFNHPEEVRKHREKVNTTSEPGAELRSPLTNDHNGSASPLHRPDTPTSSSVIDPQDYHYARREAVISKLNGTEVNLDEMDEAEFEKLFDDVVRLRMQRKVGTSVSGRESRMSYISEEEDILSASHRPPSGSTFVTDDTSVAGSIFNDLELTIPEEGNQLKRSPSDLASNGAIETEHLRLEKEQVENQMKELKEEMEQKLEVQKQMFQKKVKKLRTKSMQFNHNDLDIAFELEDWTPQERHLARISIKRWKKLNRVKIAEKLLRKAVILKEANVISRELNKMVTYQFIIVDRSPLELTTSALESIKGLSEFDDVSDPELSDRSNRPCIGIKVLDKRHRSVYLWSLDKLETRVSQMRNLFNFIDKPEFSQHFNWEDPFYDPSPPLYSFIAHAFIPLGPIYRNTPIKTTLSLYSPYTLASIGTCRIQIRTIGIAPPARTGILLNSPNGTIQPTVNESLCPLVDDAKITLEIIIDEVVGLTSRDFSSVHCQFRASSITGVLKDGTEDHVWSSKAFDLPCDQKEMVLKQTISVVMSPQTKSHLAHGCAPIEFFGQLMPSYLDRLERQDEKKAEEKKLLDDNGGRSELLSPQSDSACPTLNQQSTASIIRMRQPETEFVVEQMHDVTSAIKMLELTALGDYQSTTIISQNALDPGVFYLRQGLQRKLAIRLTHNSGRQFGWQRMEKVEMGNVRLLDGQGRIHSSKVDRWVSIKASKKPVVHFEADGTSWMDYVGNWDAGGHESHYLDRVTQSEQRVLVELRWEVEAKNCVEPIKFRTDIGMVVKGRDQRVGEGILSLLRIERRLKQMNYLFSIKLKPMMISKVDELWRLSTVNQIVHGEERLKGWKTRGLGLLTEWINFRDSILLKTEVDGIKAIVTSLTLKSINELDERLRNPEPGAADLFKIGKSLELWQKRFGPKGNMNFLEEPQLIVLDQQPIESSSTPKQTFESPKNRRLKAEVRLIPRHENAIRKGKLNVLRDPTRDDWVNRWFVLKRPYLYIYEKNDEVEELGVINLLNVHIDSNPEMELMLNRKNIFAIYSSTNSYFLECLNPKDLAEWCLAIKPLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3KTD5</accession>
    <name>E3KTD5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_13931</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. ARD1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178307">
        <property type="protein sequence ID" value="EFP87560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331979.1">
        <property type="nucleotide sequence ID" value="XM_003331931.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KTD5"/>
    <dbReference type="FunCoup" id="E3KTD5">
        <property type="interactions" value="295"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KTD5"/>
    <dbReference type="GeneID" id="10538620"/>
    <dbReference type="KEGG" id="pgr:PGTG_13931"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13931"/>
    <dbReference type="HOGENOM" id="CLU_013985_7_2_1"/>
    <dbReference type="InParanoid" id="E3KTD5"/>
    <dbReference type="OMA" id="MSMQNAN"/>
    <dbReference type="OrthoDB" id="25586at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990189">
        <property type="term" value="F:protein N-terminal-serine acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990190">
        <property type="term" value="F:protein-N-terminal-glutamate acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045047">
        <property type="entry name" value="Ard1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091:SF4">
        <property type="entry name" value="N-TERMINAL AMINO-ACID N(ALPHA)-ACETYLTRANSFERASE NATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="4">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00025786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP87560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="110" mass="12510" checksum="06072FFFE0D4BA57" modified="2011-01-11" version="1">MDIRQATKGRVVGYILAKMEEDPADEPHGHVTSISVLRTYRRLGLANKLMQQAQKAMRDVFGAIYVSLHVRKTNRAALSLYQDTLGFAVKEIEKKYYADGEDAYSMRMVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KTI9</accession>
    <name>E3KTI9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13985</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178307">
        <property type="protein sequence ID" value="EFP87614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332033.1">
        <property type="nucleotide sequence ID" value="XM_003331985.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KTI9"/>
    <dbReference type="GeneID" id="10538675"/>
    <dbReference type="KEGG" id="pgr:PGTG_13985"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13985"/>
    <dbReference type="HOGENOM" id="CLU_1540835_0_0_1"/>
    <dbReference type="InParanoid" id="E3KTI9"/>
    <dbReference type="OMA" id="LMAPNSI"/>
    <dbReference type="OrthoDB" id="2501222at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="157"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="175" mass="19312" checksum="9F18636A02D1BAAC" modified="2011-01-11" version="1">MCSRSSSCWEEHLETQTDDFRPNNTPGPLSIAIFFLGFIVLNHSIRRLFLSGAAGREQLPNPDDSRESNKRDRADDDGHGKEEEGTTSSDLMRMMLVQHQHPSSRLPSNPFARALLPDPAISISATPIASTPLPNTWVDSHRNFRQPFHSAGFRSSAAPNLSEFSDSCSESTTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3KV62</accession>
    <name>E3KV62_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glycosyl hydrolases family 2 sugar binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_14369</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178312">
        <property type="protein sequence ID" value="EFP88285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332704.1">
        <property type="nucleotide sequence ID" value="XM_003332656.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KV62"/>
    <dbReference type="STRING" id="418459.E3KV62"/>
    <dbReference type="GeneID" id="10535676"/>
    <dbReference type="KEGG" id="pgr:PGTG_14369"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14369"/>
    <dbReference type="eggNOG" id="KOG2024">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1138483_0_0_1"/>
    <dbReference type="InParanoid" id="E3KV62"/>
    <dbReference type="OrthoDB" id="408320at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051913">
        <property type="entry name" value="GH2_Domain-Containing"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006104">
        <property type="entry name" value="Glyco_hydro_2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42732">
        <property type="entry name" value="BETA-GALACTOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42732:SF2">
        <property type="entry name" value="BETA-MANNOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02837">
        <property type="entry name" value="Glyco_hydro_2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172856" description="Glycosyl hydrolases family 2 sugar binding domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolases family 2 sugar binding" evidence="3">
        <location>
            <begin position="103"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007401"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP88285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="244" mass="27167" checksum="0F8239D911B2CDC2" modified="2011-01-11" version="1">MATLSIFLHGLTMLSFLGSGGATAVSDCLALKPYQLKTPPLTTDWTEKVGISPWPEYPRPLLRREDWMNLNGPWQFKPAKDSQDIHRPPFGGCGFASEILVPFPMESGLSGIMKNNMYSWYRKTFVVPEGWSGSNVLVNFGAVDYEATVFVNGKTAGFHRGGYFRFALDLTDALNEPGEENELLVFVYDPTNSNGTLIPTGKQVLDPSHYFYTPSSGIWQTVFLEPVPKEHILEIVTTAVYGLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVK9</accession>
    <name>E3KVK9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14433</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178313">
        <property type="protein sequence ID" value="EFP88349.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332768.2">
        <property type="nucleotide sequence ID" value="XM_003332720.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KVK9"/>
    <dbReference type="GeneID" id="10535821"/>
    <dbReference type="KEGG" id="pgr:PGTG_14433"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14433"/>
    <dbReference type="HOGENOM" id="CLU_2005025_0_0_1"/>
    <dbReference type="InParanoid" id="E3KVK9"/>
    <dbReference type="OrthoDB" id="10273195at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172236" evidence="1">
        <location>
            <begin position="23"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88349.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="124" mass="13804" checksum="8BD367662ACC0CC8" modified="2012-04-18" version="2">MMMSIGLPILLLVLLTAASIHGCPTDTHAVCFSPNGYKNTDPSINYVEAKFPPRNKGCNIYLHIDHAVGPDRKFWRTRCCKKEALDKIRPPPPYNTQGTFRIPKATLVHGDLDSPPPRGSQDCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVP9</accession>
    <name>E3KVP9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14473</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178313">
        <property type="protein sequence ID" value="EFP88389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332808.1">
        <property type="nucleotide sequence ID" value="XM_003332760.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KVP9"/>
    <dbReference type="GeneID" id="10535862"/>
    <dbReference type="KEGG" id="pgr:PGTG_14473"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14473"/>
    <dbReference type="HOGENOM" id="CLU_051119_1_0_1"/>
    <dbReference type="InParanoid" id="E3KVP9"/>
    <dbReference type="OrthoDB" id="2505270at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027532">
        <property type="entry name" value="Mdm12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204:SF1">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="291" mass="32845" checksum="F8E270BEBE8BD29B" modified="2011-01-11" version="1">MSKNDSVSETVTQTTRFLLTSSNYTIWVLPMSAKLEDIGIRTYVTGVLKTDEKTSADELAQIEKLNVKAYSMIIQNLDSENLALVSTTLPPSNHFDGKALWSLLRRKYAGSDLAARSVALDVFLDLEYEDVSTFCNSIRLANQKLLLAGIMLDDQVKMMIMLRKLPREDFRSFRDIVAMGFSTESFEAIVKRLESYSVTNKISKDNKKSSIEPRMTLHTLAQPSSQPSDPTPSVSPVCVHCKKTGHRPTNCWVKYPEKAPKSPHSSHYTHYIHASGELRPIDEHCPQEVRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KY34</accession>
    <name>E3KY34_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_15076</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178320">
        <property type="protein sequence ID" value="EFP89235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333654.1">
        <property type="nucleotide sequence ID" value="XM_003333606.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KY34"/>
    <dbReference type="STRING" id="418459.E3KY34"/>
    <dbReference type="GeneID" id="10546872"/>
    <dbReference type="KEGG" id="pgr:PGTG_15076"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15076"/>
    <dbReference type="HOGENOM" id="CLU_018552_2_4_1"/>
    <dbReference type="InParanoid" id="E3KY34"/>
    <dbReference type="OMA" id="ATEDECA"/>
    <dbReference type="OrthoDB" id="2505664at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP89235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="209" mass="23927" checksum="31C08852972DB246" modified="2011-01-11" version="1">MRKILRLHITQAASETRYKESDEDSSTSEDSEIEELVMTLISIKKKRYLAERVRLERAPDITKYLFRLDTGRFKQEFRMSQDSFYQLLDLIKDNSVFHNNSNVPQRPVRDQLMVTLRQTGMSGNGSSVGVLARFFRILEGTVILYCSRVVEAILALESTYVVWPNHEAREAIASDIADSTGFKQCVGFIDGTLLPLDEKPSIDAQDYYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KY41</accession>
    <name>E3KY41_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6589 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_15083</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178320">
        <property type="protein sequence ID" value="EFP89242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333661.1">
        <property type="nucleotide sequence ID" value="XM_003333613.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KY41"/>
    <dbReference type="GeneID" id="10546879"/>
    <dbReference type="KEGG" id="pgr:PGTG_15083"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15083"/>
    <dbReference type="eggNOG" id="ENOG502SCRC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009176_0_0_1"/>
    <dbReference type="InParanoid" id="E3KY41"/>
    <dbReference type="OMA" id="AIRIMIN"/>
    <dbReference type="OrthoDB" id="2509760at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046496">
        <property type="entry name" value="DUF6589"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20231">
        <property type="entry name" value="DUF6589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6589" evidence="2">
        <location>
            <begin position="364"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP89242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="748" mass="84102" checksum="CE64DEDF27F6BA20" modified="2011-01-11" version="1">MEIDDFQSLSPPKQPRSPQAAPSDTLPTKIVEFCDFLKSNYNLTPKKFVVAFLTADHDTLAFRRRYWGSSTGWPSTLDLIQTIKKQVTKTQKGQDFWREFIKGEAIQILDKEKPPTGNYPDGCFQSSQTVEPSFFDHDAACIREESMIKHHMPFLFDTLMGTLQSSDEMQTSTNTTVDPDTEPPSDPNGVDMDEQEMMEMDGTQYENKAIPCRIKKIAATICAQVAFAKNRRCNGLQLFNSVRFMAGNVSEAVNDYLHHIGLTSSRQTAVRALISSSARAGRGLRTSSSIKNNPIISPSICIDNLDIEQRVHTHGVGHRSMMFHGTWGYIHKPNPSLLASLDHSQLTLEAYNRALSEIPTLKIVPSMFLPTAEEDNHFELVMKSQISRVMRQYIATPINNKSAIPSNPPEIDLIDCSPPDIDMLKLMDASDNSADGVGQVLGSIIQQLGLTADEFCSRLQIMDGDLGTCQNINSLRALRSPSSYADHALLNITMQLGSSHTLWNISQNILTSHFGDPSKTNDLGTWQYWHALGIPSDKAASKKDFTLMMKSIEKAHEATIFYCLRVIMQTENEPVNGDVHRIPTDEWNDIIDECYSQFLSPQARRNVSKAESPKLHSILIRLHDFATIVEANRAMKAGDIGRLMNMWKIWSIMSQALTGLVNYRSYLPRMVLLLTHILPPSLSKYIRHNLLISPSGRPNHFVAKDYYLECQNYMLKFLYNRSGVGTQISTLQDLFSLNMTMVSNPPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZ95</accession>
    <name>E3KZ95_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15583</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178324">
        <property type="protein sequence ID" value="EFP89620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334039.1">
        <property type="nucleotide sequence ID" value="XM_003333991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZ95"/>
    <dbReference type="GeneID" id="10547673"/>
    <dbReference type="KEGG" id="pgr:PGTG_15583"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15583"/>
    <dbReference type="HOGENOM" id="CLU_1993744_0_0_1"/>
    <dbReference type="InParanoid" id="E3KZ95"/>
    <dbReference type="OrthoDB" id="10576967at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="74"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="125" mass="13040" checksum="432E701AADD58686" modified="2011-01-11" version="1">MGFGKSYGHNTVCPIGLGTQTSTLKGQTVTPKSSPVNKGRKFITFEEKGTLPVGEQQEQDHGSNSIQSGTIVDQGQGTSSGLTQGSVVESEEEVPLALLVDIKGKGKMVESVASAKMKRKGGGEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZG4</accession>
    <name>E3KZG4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15652</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178324">
        <property type="protein sequence ID" value="EFP89689.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334108.2">
        <property type="nucleotide sequence ID" value="XM_003334060.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZG4"/>
    <dbReference type="STRING" id="418459.E3KZG4"/>
    <dbReference type="GeneID" id="10545256"/>
    <dbReference type="KEGG" id="pgr:PGTG_15652"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15652"/>
    <dbReference type="HOGENOM" id="CLU_025212_0_1_1"/>
    <dbReference type="InParanoid" id="E3KZG4"/>
    <dbReference type="OrthoDB" id="2506374at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033018">
        <property type="term" value="C:sarcoplasmic reticulum lumen"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051279">
        <property type="term" value="P:regulation of release of sequestered calcium ion into cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10033">
        <property type="entry name" value="CALSEQUESTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10033:SF0">
        <property type="entry name" value="CALSEQUESTRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89689.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="459" mass="51565" checksum="12F2B09CA10FEDF4" modified="2012-04-18" version="2">MPETRSTSNSEGRQTNGIGHGGCGSRGSRGSRGSGGGRGGRGGRGGRVGGDSSRGSSDRESSDHEEHSDSDEIAFTLENYENHMSSWTDKQLRDVLGKQTRTSNRIPTEVQEALLFHKKNYCKIKLMLALIGNVSEKTVNSWLGEDQPPEERAMESVCSFQHGKLNLSPVPPKGCSEGWEERNVHLGQAWAALSDDEKNVFDEKIFRFFSKLPTSLDNHDTEEEEEEESLTPEEEALYRPLYQRLVNHEKVEFLAGNSQAPDNTSVVSQRALKHIIRINSELFTVSNLYNLTFYLLSATRFPGNGSFCEELSNDPSWLQIAKSKWFAKENFEAYSHGRNIQQVLQESSAETNRPAKRPKHADTVRTQLRQELNKLLCLALGVEYDPSPKKKGPVFPKQKDLVTLLEKEHPAVKIVQLENSTLKVDELAIGLEYMRNPVREKWLDDIKNGVFTLVSDTSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZH0</accession>
    <name>E3KZH0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_15351</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178325">
        <property type="protein sequence ID" value="EFP89695.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334114.2">
        <property type="nucleotide sequence ID" value="XM_003334066.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZH0"/>
    <dbReference type="GeneID" id="10545262"/>
    <dbReference type="KEGG" id="pgr:PGTG_15351"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15351"/>
    <dbReference type="HOGENOM" id="CLU_035165_0_0_1"/>
    <dbReference type="InParanoid" id="E3KZH0"/>
    <dbReference type="OrthoDB" id="2502487at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="267"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP89695.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="332" mass="37088" checksum="A9F13D35147AED68" modified="2012-04-18" version="2">MSFKWTLVDIGDESGTGSRKVNFVKSTYQFQDTNYQYIVCGTCLIGNTLKAKLCNSLNAAADDLLRGSSGRFWASSAHVYRSFDGYDPRLSIPLHTLPLSAFPPNYTEVQEDYYRSFKPTIGPHDITELGTWLGGVRLIPASQLSGYDVEFPWRTLPPPQGNIFVDNSTEPSDFFSAFNTTYHALCQGHECSNFRITVNGIGALGPFGFKDSKQFKIPEVLMDMFNRSLSYDVRIMDLAANDLHGKEIGATYLCTKTTKKWLPLLKVISVTLGSSSGLFSGIFSYVIVALARRYDKHHENRRNSQATQTRMDESTLNSEDDKDSSLGAKNLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZV4</accession>
    <name>E3KZV4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_15791</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178326">
        <property type="protein sequence ID" value="EFP89835.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334254.2">
        <property type="nucleotide sequence ID" value="XM_003334206.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZV4"/>
    <dbReference type="GeneID" id="10545567"/>
    <dbReference type="KEGG" id="pgr:PGTG_15791"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15791"/>
    <dbReference type="HOGENOM" id="CLU_026798_0_1_1"/>
    <dbReference type="InParanoid" id="E3KZV4"/>
    <dbReference type="OrthoDB" id="2505955at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="111"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP89835.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="376" mass="42918" checksum="F34CBDED22D5E40F" modified="2012-04-18" version="2">MLKNKKRKEGCKKRWRQKTRKASGNEASTEIKKGLYQFTARPSPVSLYDEYRQRKKKKYLTPASILQAANFIKAPGFRIFNRPDSHVMIFDEYNQNRLVGIFQFTPFSKMTPDQREDLDFLAGFFHSHKKYVNPVSNFNSACLGGKMNMLGWRKCMKPNERAGLFLSQAKINKDVHGFTSVVRRGHQAGVIIGKSFKDLADNAFAKNHDIMVEYDMPSFGDATLDDLEVNNFSAASSLSYTYGGFYNSPHTDNQDVSEFAYVQWIPTFAKTGKVATHAEGFNVVGGEFVFPDCRFGLGFENLDGVARMVWRSTDYKHFTMFSQPNSTFNRLAFSLQLNKKTVNVFKNIKTQEGAYLNMHDGDLNYILATAEKQKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3L024</accession>
    <name>E3L024_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">OTU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16199</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP89911.2"/>
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        <property type="entry name" value="Papain-like_cys_pep_sf"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="OTU DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
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        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="25"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="672"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="694"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89911.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1543" mass="173562" checksum="3AA3FD41B1664090" modified="2012-04-18" version="2">MPMGVGNGEGAPQANVALQEAVQDLPGGKRKNVVEEGASKSQANAMIGASNFSPEEVIQILGRPIKVEKGVPAPKQLITGVKGGSGQDFVTPRSPVTSEESDWGPMIDNLLGQKADDVTEEESVDEWWDAVEQESQVSVECGISASKNNSDLGPMTDGPITDFQEWMYNLPIPDEEDYDLDSWAISIETHQLSTVLHPMGLVTLAHHVLALQDHKDFSDFVFVAQGACTIRMPPNFKERVLQKLWNNFHIIAHPSANPRILNMGSPSCTVRMLQEVFHQLRVPTLRLASYGMKCPFRLVTHNLGEGVRDFMTINQRKNHFDIGFTFFPYYGTDQFRHQILLARRPPGTRRCRSESYPTLLELGEGFGTVNLQTEHEAITRLKCYSSLTHALKATSQKDMKHAPTTAMTWNTRLKTFEKKRDILAANDPAHLCGLRFEATVYASTVDEAQEIVRRSGYLHPRAYRHGPENPNEQIALDFKAVPVSEYFAYVALMLKKLKRMQTGVSQKHETQRKITDIQKQAIVDVANALGWNGGAGQRPTSVDREKVPDPWWDEYDDSWEERRLKEGEVTLGNLLRVPVLDLWQLRPRRVFPCSICHALDNPSPPKPDKSKFYPPGSFTSVGGTKHFKLKCNGCGKTTTSAQEAVNMLATWIEKLGLPLLGEHRRRTLAGGYCLDNPEKNPSLSPPPKTVTIESTEKSTPEPEKKKGERIPVVVIPKLGPIVVKAKKPQTENIPAKATTASLSDLVNKDIVELASQANNVAKSKLEVVVAQKTTGTLLGWVGFADYLLKNNLWEDSSSALPSTQAIPTVSSMDQLDNWKLSNGAVAIWVLGDQEKHGEIRKTCWRELVENQDEYAPYVTEDDLRNPGNPVPQAIVDGANIYDVFLEKSLTDRFWGGDKHLSALAQALGIRIVVLTQQRGTFGYQPRTRQPEKTIYVWYNGGTHYEIIWNKKLHRPQSAKKKTPQKNMLPGKPLLKPPSIAKSVSNVIPDAPKGKRVSFGTLVLEKTMTTGKTMMHIDPRLQSPPNSNAPSISTIASIRKSQDQNPTSPKNSTPLKHPNFVVPPTFAFAGTHPLPEKLKKKKRNRMPGLEKSHGLPVVSGYWNTLNERLRLETGRNKKTLPYGVIRFAIPEGLLDGWRISHDFIQTSRSVSAPDITGFYHAVGYWMTGTQDGHAIFQKRLADQLGGQLPQMSRDQQKSSRLVNVENKNLFSVGEIKKAFQLVFDGKKPDKVVYTTAATMLRVTFVEVVMGPSPANWMQYSPTGYQNTAFPCLYLIFNSISRRFQIMWAPKIHQPVVGPENADLQTRLSGNTTLFSDTQSPPSLHHILPVQIYQPSENSTWQTSNCDTSPPQKRFKIIQTGQKPILTLESYSMPTIEDLKTVDFGPASGIHIFHQKKSCNPQCRAVAKTAYFELGNFKYVGWRFSINLFPDNKDSIWRALSFWIYGSQGRHNEIRRSCESRTHVLNNPLEDPPEFHYSEIPGLLREVSNRYRARILLVWIHDGIANLQEFNPRYIDEDDERLVPRNYFIYSWKGRFGIFYCHHHL</sequence>
</entry>
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    <accession>E3L2I5</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    </protein>
    <gene>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178337">
        <property type="protein sequence ID" value="EFP90818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335237.1">
        <property type="nucleotide sequence ID" value="XM_003335189.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L2I5"/>
    <dbReference type="GeneID" id="10530722"/>
    <dbReference type="KEGG" id="pgr:PGTG_16844"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16844"/>
    <dbReference type="HOGENOM" id="CLU_1787779_0_0_1"/>
    <dbReference type="InParanoid" id="E3L2I5"/>
    <dbReference type="OMA" id="HYPYLIG"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="145" mass="16539" checksum="1642E84EB072F3DB" modified="2011-01-11" version="1">MTKSTRSNRPELRGLLPAPTRPRGSKGAAIESRESQDDHDNTQRNNDEVSRKGNADPRRPASDYGEGYGNGSRRDRFRMLGSYPETPYNSRDQSLIETRPDQTPGQRARLALETIQNLLKFKRNDHGKKQSSRLDQFADPGKWKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3L304</accession>
    <name>E3L304_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_17201</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178339">
        <property type="protein sequence ID" value="EFP90929.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335348.2">
        <property type="nucleotide sequence ID" value="XM_003335300.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L304"/>
    <dbReference type="STRING" id="418459.E3L304"/>
    <dbReference type="GeneID" id="10528343"/>
    <dbReference type="KEGG" id="pgr:PGTG_17201"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17201"/>
    <dbReference type="eggNOG" id="KOG4313">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048013_0_0_1"/>
    <dbReference type="InParanoid" id="E3L304"/>
    <dbReference type="OrthoDB" id="10261522at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044715">
        <property type="term" value="F:8-oxo-dGDP phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03676">
        <property type="entry name" value="NUDIX_Tnr3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000019">
        <property type="entry name" value="Thiamin pyrophosphokinase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13622:SF8">
        <property type="entry name" value="THIAMIN PYROPHOSPHOKINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="259"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="65"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP90929.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="439" mass="50344" checksum="2D6ACFD884E6843D" modified="2012-04-18" version="2">MSFLPVLKTCHNHPTLNPLLQQTNSNNNNQQQQLIPFYLHQPDQQQLIKPIRLPPPPPPPKRLTNRSLSSSSSSTSNPAELTKFRPLGSYLTRSNSSDQLDNHPFNEPIYQPPWDQAISDRDSRQPIGFLRPAIIKALIQDNQHMNRIHSQACWKLFYPIETDDDQDDLGDRLPVKVSFEDWINQAEDRIETRQEHLDRLIRGWKENGLFLDQLSGWRNEEYSVYGPKDDDPEESDHEPTQLPGSNLAFRIERAAVGLFGFLSFGVHLTAYVKKDGEYFFWIPRRSATKATWPSKLDNTVAGGITSGETGFETIIRESFEEASLEEELVRTHIRATGLISYTHRSPEGWVQPEIQYTYDLELPDEKTIVPKPNDGESEDFKLMSFDEVSEALKRAEFKPNCAAVLVDFFVRHGLLSEHNEPHYFAVSTLVKQPVFLPLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3L2</accession>
    <name>E3L3L2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_16882</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178342">
        <property type="protein sequence ID" value="EFP91137.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335556.2">
        <property type="nucleotide sequence ID" value="XM_003335508.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L3L2"/>
    <dbReference type="STRING" id="418459.E3L3L2"/>
    <dbReference type="GeneID" id="10528799"/>
    <dbReference type="KEGG" id="pgr:PGTG_16882"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16882"/>
    <dbReference type="HOGENOM" id="CLU_011407_5_0_1"/>
    <dbReference type="InParanoid" id="E3L3L2"/>
    <dbReference type="OrthoDB" id="3253684at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041320">
        <property type="entry name" value="CxC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040521">
        <property type="entry name" value="KDZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18802">
        <property type="entry name" value="CxC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18758">
        <property type="entry name" value="KDZ"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxC1-like cysteine cluster associated with KDZ transposases" evidence="2">
        <location>
            <begin position="160"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="58"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="97"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP91137.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="830" mass="95703" checksum="A6CE129F8CE57F9C" modified="2012-04-18" version="2">MPLFEGDGGKRRKQIRKPKTEAGVSFSQAVQLEHQHILQTPLAQPANDIHPTDHPNEDSSAQENNNQTIDPTGQDNFDFGADHIGNNGLDPFQSAHQQDRLPSDHQDNDHLTNSRVQQLHRYFQSEHYKQKKILEEKNWEEAYQEMFSSFQQCAIKTFEWGNPNLWNHDWNEPCNCNNLRSRPVVLVDMYSRTQTEVTFCKCQSDQVRLIRMGYIGGSPKFPRTAFSIRLLRFHHIIWKHCAVSITPFSKALDEFLDSNNPLILVSPPTLQSESCYTTRQWRQTISAAIDAYREMIRRGQIILEELLQMGPMDKLANICPKCFGPPVPGKTPDEPDYILCMDGNFQHRRHLSASQELHQSRKPTTLFISPAEVQEMELSMDRCTEQHTAANDTRGAATWKACDDTGIFGLACRHDQILRLINIVQSGENEERQGNQLMFGTSFFHAYVHQWSCQLQYNPRLNTGWGMSDGEGMERIWAYLSPLISSLRYSTKTHRLVALDLRSSHHNDTGKTNSVRLLLDRGKQVEEAMKTALEKLKEIEEDFGHSTVYLADQWIRQRECQLSVMETESERDMMKQVEELVELEDQLWDTHDTIVSLRAKRRRDRTSEEQRQLHGLPDTVVSLEEQIDQVISELGSEAFRGLPGASDAETKALIRLKVSKSKLYEAKVGVLEVQKRWDQRGSGTRMQARFKKLMSSKVKLLKSKWTSYCNRATIYNENYMPEIDVGTPTLEEIKSMGLDDHFWDMGSLLHPNEPWAIDPNVREGIDALLTSTHCKDELRRISREARQAVKWAIDRSNSLENLYDLLDPVCLLLTRQKSQSSFDDMERQLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3T7</accession>
    <name>E3L3T7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Large subunit ribosomal protein L5e</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_16403</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178343">
        <property type="protein sequence ID" value="EFP91212.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335631.2">
        <property type="nucleotide sequence ID" value="XM_003335583.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L3T7"/>
    <dbReference type="FunCoup" id="E3L3T7">
        <property type="interactions" value="470"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L3T7"/>
    <dbReference type="GeneID" id="10528875"/>
    <dbReference type="KEGG" id="pgr:PGTG_16403"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16403"/>
    <dbReference type="eggNOG" id="KOG0875">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056222_1_0_1"/>
    <dbReference type="InParanoid" id="E3L3T7"/>
    <dbReference type="OrthoDB" id="1618453at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00432">
        <property type="entry name" value="Ribosomal_L18_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.100:FF:000002">
        <property type="entry name" value="60S ribosomal protein L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01337_A">
        <property type="entry name" value="Ribosomal_uL18_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005485">
        <property type="entry name" value="Rbsml_uL18_euk_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057268">
        <property type="entry name" value="Ribosomal_L18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025607">
        <property type="entry name" value="Ribosomal_uL18_C_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410:SF12">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410">
        <property type="entry name" value="RIBOSOMAL PROTEIN L5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14204">
        <property type="entry name" value="Ribosomal_L18_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17144">
        <property type="entry name" value="Ribosomal_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00058">
        <property type="entry name" value="RIBOSOMALL5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL18 C-terminal eukaryotes" evidence="7">
        <location>
            <begin position="347"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="355"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="366"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF14204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP91212.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="408" mass="45346" checksum="7E3E0ECFC57D5E93" modified="2012-04-18" version="2">MILASSCLYPPAQPLQTGDASFGVVLLFLLAGPLTLSMGLNCDQDGVSCRLNIHMYPPLRGLSLISPGGDRPPGYVAHPVRRLVGSGRRPSTVHSQVSHPPSSRPSFPFLKMPFVKVVKNSAYFSRFQVKYRRRREGKTDYYARKRLITQAKNKYNSPKYRLVVRITNRDIICQVVHAKLQGDFVLAAAYSHELPRYGIKHGLTNWAAAYATGLLVARRALVKLGLDEKYQGVEEPEGDLVLTEANDEGPRPFKAFLDVGLRRTSTGARIFGAMKGASDGGIFVPHSENRFPGYDPEAKELDADTLKSYIYGGHVAEYMEALEEEDDERFKKQFSTYLADDIGSADIEEIYTEAHKAIRADPSPKPTDKSKLEEYKKASKQNKPSKITKEQRAANVKAKVEAYMAASA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>E3L4W8</accession>
    <name>E3L4W8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Calcineurin-like phosphoesterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_17647</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178349">
        <property type="protein sequence ID" value="EFP91593.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336012.2">
        <property type="nucleotide sequence ID" value="XM_003335964.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L4W8"/>
    <dbReference type="FunCoup" id="E3L4W8">
        <property type="interactions" value="206"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L4W8"/>
    <dbReference type="GeneID" id="10531530"/>
    <dbReference type="KEGG" id="pgr:PGTG_17647"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17647"/>
    <dbReference type="eggNOG" id="KOG3662">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_464304_0_0_1"/>
    <dbReference type="InParanoid" id="E3L4W8"/>
    <dbReference type="OrthoDB" id="5977743at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.10:FF:000103">
        <property type="entry name" value="Unplaced genomic scaffold supercont1.18, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033308">
        <property type="entry name" value="PGAP5/Cdc1/Ted1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13315">
        <property type="entry name" value="METALLO PHOSPHOESTERASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13315:SF4">
        <property type="entry name" value="METALLOPHOSPHOESTERASE, ISOFORM E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="25"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="392"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="668"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="7">
        <location>
            <begin position="101"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="421"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="517"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="437"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="469"/>
            <end position="478"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP91593.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="688" mass="77889" checksum="7F25B6082DA7AE04" modified="2012-04-18" version="2">MSPHQPSRAEKQPIYHPRRLDRSLLILRIVWIITIVWGEWIYFDRTISSCQWPRPTTTTTTEPFHLLIIADPQLPSTDYSYPDRILPLRWLSIKIIDQFIRKSWRLLIKNTKPHAVVFLGDLLDGGIAASDPAKFQTYVDRFYHTFPIPADLSSAPTSNQTEPPARLIHLVGNHDVGLYPSTSYERSAQARERFKNTWPPGLLNGHVEWANHTIIWIDALSLIEESKRRAAGLSTQEDGQVTRFVKELAGADMLLPKVLLTHVPLWRPEGTSCGPLRESRRDIRQGAGINYQNEIPEEATKMVLEKIQPSLVFSGDDHDYCEVIHTLPSTSLANPSPLSIHEISVKSFSMGMGVQEPGYQLVTLSNPGRFGRAPDEQTTFHRPCLLPNQIRIYTHLYLPLLLMSLVVLFGPEIWRRIRRRTRGGTSREQARTNGLPIHHRAQRHRKSLSRTLMFTKPISSSSSSSSSADEARSDEEAGPRFASHRARRSASLGLAIGLGIDHGDGFHSPSGLLSYHSPVDSGHDDQRFSHPSPALPFDHSHKLRRSVELTQNSTELSSHYPSHHHSPNAYPPTHLYGRSLSSRFQSSSSIPSFSSLSSSSSFSSDHLSLKLLRLFLGHQLSERLRNWLINHRFISPSHSTGTSSVFSKHRRRKKNNILSPLCSGLRRLFGLVWVVLLVFFSVWFSFDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3L585</accession>
    <name>E3L585_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">lytic cellulose monooxygenase (C4-dehydrogenating)</fullName>
            <ecNumber evidence="15">1.14.99.56</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PGTG_17447</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>[(1-&gt;4)-beta-D-glucosyl]n+m + reduced acceptor + O2 = 4-dehydro-beta-D-glucosyl-[(1-&gt;4)-beta-D-glucosyl]n-1 + [(1-&gt;4)-beta-D-glucosyl]m + acceptor + H2O.</text>
            <dbReference type="EC" id="1.14.99.56"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the polysaccharide monooxygenase AA9 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.99.56" evidence="15"/>
    <dbReference type="EMBL" id="DS178351">
        <property type="protein sequence ID" value="EFP91710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336129.1">
        <property type="nucleotide sequence ID" value="XM_003336081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L585"/>
    <dbReference type="STRING" id="418459.E3L585"/>
    <dbReference type="GeneID" id="10531648"/>
    <dbReference type="KEGG" id="pgr:PGTG_17447"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17447"/>
    <dbReference type="HOGENOM" id="CLU_060387_0_0_1"/>
    <dbReference type="InParanoid" id="E3L585"/>
    <dbReference type="OMA" id="ACQNFDA"/>
    <dbReference type="OrthoDB" id="4849160at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.50.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049892">
        <property type="entry name" value="AA9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005103">
        <property type="entry name" value="AA9_LPMO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008427">
        <property type="entry name" value="Extracellular_membr_CFEM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33353:SF10">
        <property type="entry name" value="ENDO-BETA-1,4-GLUCANASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33353">
        <property type="entry name" value="PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12560)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03443">
        <property type="entry name" value="AA9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05730">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="8" id="KW-0186">Copper</keyword>
    <keyword evidence="10" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0964">Secreted</keyword>
    <keyword evidence="5 17" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="17">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174396" description="lytic cellulose monooxygenase (C4-dehydrogenating)" evidence="17">
        <location>
            <begin position="23"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="domain" description="Auxiliary Activity family 9 catalytic" evidence="18">
        <location>
            <begin position="92"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="CFEM" evidence="19">
        <location>
            <begin position="309"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="226"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="266"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="268"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="293"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00044502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00045077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF03443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF05730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="EFP91710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="386" mass="41449" checksum="1FCC567A8182FF5F" modified="2011-01-11" version="1">MCGPKLILFPVLYFVLVPAVAGHGYVKSWASEDLKFQKAQKQSLSDTAFRNAPTNIGWIGSQFINTPAFVCGASETPHDKVAPPSGTLFSAADQSAKKTLAVNAGSKVSLIIGGNQGEGFPHPTGHMLAYLGFCGKSPTACQDFDASKADYHRIQAEIDGISNKLRKQFNSEQDGHRWDVPIPKDISDGSYILRIEMIAFGQSSPVEGHQDQYYVFCGQIAVKGGTGSSPIPDDDQPTIRLPGAFKSGNISPKSLPTPLKLAAGLSAKESQSKVQTKSLNPDGDSNEKKSSDSSDPPESSDSKPIVTSVCGDRCYKKKITELKDLAPSCSADQFTCICKSQSFVKAYQSCCNDHCQGVRETRSAVNEIYVKCESAQSPSDSDDNSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="83" xmlns="https://uniprot.org/uniprot">
    <accession>E3L5X1</accession>
    <name>E3L5X1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Zn(2)-C6 fungal-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_18397</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178355">
        <property type="protein sequence ID" value="EFP91946.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336365.2">
        <property type="nucleotide sequence ID" value="XM_003336317.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L5X1"/>
    <dbReference type="STRING" id="418459.E3L5X1"/>
    <dbReference type="GeneID" id="10531898"/>
    <dbReference type="KEGG" id="pgr:PGTG_18397"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18397"/>
    <dbReference type="eggNOG" id="ENOG502RYE1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_305663_0_0_1"/>
    <dbReference type="InParanoid" id="E3L5X1"/>
    <dbReference type="OrthoDB" id="424974at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00067">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.240.10">
        <property type="entry name" value="Zn(2)-C6 fungal-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036864">
        <property type="entry name" value="Zn2-C6_fun-type_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001138">
        <property type="entry name" value="Zn2Cys6_DnaBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF56">
        <property type="entry name" value="ZN(2)-C6 FUNGAL-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00066">
        <property type="entry name" value="GAL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57701">
        <property type="entry name" value="Zn2/Cys6 DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00463">
        <property type="entry name" value="ZN2_CY6_FUNGAL_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50048">
        <property type="entry name" value="ZN2_CY6_FUNGAL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zn(2)-C6 fungal-type" evidence="5">
        <location>
            <begin position="38"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="15"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="105"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="183"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="697"/>
            <end position="809"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="866"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="105"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="704"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="722"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="749"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="771"/>
            <end position="783"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="876"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="889"/>
            <end position="904"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP91946.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="917" mass="100937" checksum="C2A079ECA58AAEFD" modified="2012-04-18" version="2">MDSIIKSESVYPAFSEPKIPTTTNLPTPPKRRPRHRLSCAECRRLKLKCDRTWPCSSCTKRGCARICPDGTLQSKVRTKDTSVLLARISELENLLQRQDHQLRLSCSNDRPGEHQSTSQPSSSEYAPMGIVGSSSEQPQDTHVLALIDGIGTLNVSDDGRSRFLGLSASSAFFDGDWSDCDSLSSTPGTSEDPNETSDSPSPFTFQALLGRPALDPNQLKTYLPERHEAERAWARFSMKCTMSIATKSVSEDWHYYTPYCTSLSVLFDPTVQALHPDARKWHDAAETCLRSTDYLASPSLAIVQAIHAMGTYVMNSRADGAETFWPVLGTAMRLCQSMGLHRDGSNWGLQSSELESRRRTFHEILSMDRLQSLVLGRPYAISDKHFDTSIPRFDLNQPTESHEPNVDFHVAKWRFSSYIGRVVDDAFSVSPPSLAVVNRLDEELRQFDRALSPKLKCSNTPPICQGPSWCDMNPVERKSGSDGSIDLQLTMQQHFMTALQNTTLLFLHRRAFALALSEATAEPLNSPFSKSVLSVIIESSRNLVSVAQAAQALYPELANRWWYLFCHAYNGATCVVTLLIKSPGCILGKHAWSTLATAMSVFSTAAQFGPICRDLFNRLSRLHKQAMSALSADLLPNPHQSLSMHSYHPNQTLVSPNTSKMGEGSMALAHYLESSNPYLHHNSSDFPCELGASTRLTRKNRTQSVSNSSVASGRVSSLTSSCRSRGTSFGSTGPNEGPSAPSTIGIAANGSQGLGSNRNGSSRSPNDNNGMNSTLTTSFNMSSWAGLVNSPRDEFDEEESNEPPCWENLSIEPTDEAFNTPIFGVSHSQSNHYFEGFTEHFHPPMVLPSNDAMLSPGPSNYSGIYGPMPNPSSFDNNPNHPPVSSNHNQQQDTSATTEGMTSPINTQFDLLDTFLTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3L6A0</accession>
    <name>E3L6A0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_18165</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178357">
        <property type="protein sequence ID" value="EFP92075.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336494.2">
        <property type="nucleotide sequence ID" value="XM_003336446.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L6A0"/>
    <dbReference type="GeneID" id="10532032"/>
    <dbReference type="KEGG" id="pgr:PGTG_18165"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18165"/>
    <dbReference type="HOGENOM" id="CLU_2387261_0_0_1"/>
    <dbReference type="InParanoid" id="E3L6A0"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP92075.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="94" mass="10364" checksum="AC4B535B31503A69" modified="2012-04-18" version="2">MSEALMNQLMSVSAGESQILDGEAKDIETYGKAGQAEPSKKEIKTPNHSTYLTWKTCSKARTTGKLLPNDDDQADLKRSMLKYEQHGNAAPCFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3L6K0</accession>
    <name>E3L6K0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17905</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178359">
        <property type="protein sequence ID" value="EFP92175.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336594.2">
        <property type="nucleotide sequence ID" value="XM_003336546.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L6K0"/>
    <dbReference type="GeneID" id="10537502"/>
    <dbReference type="KEGG" id="pgr:PGTG_17905"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17905"/>
    <dbReference type="HOGENOM" id="CLU_2373841_0_0_1"/>
    <dbReference type="InParanoid" id="E3L6K0"/>
    <dbReference type="OrthoDB" id="10330915at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92175.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="95" mass="10449" checksum="ED5652F912706924" modified="2012-04-18" version="2">MQGYNHEEAQRNHRELVDGQANILRRLDNIDARLDNIEGTVSSLLRVHQNASELLRPHARFPNLNLRHRNGAVGQGNGGVGGHRGGRGGRGGRTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3L801</accession>
    <name>E3L801_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_18191</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the 'GDSL' lipolytic enzyme family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178370">
        <property type="protein sequence ID" value="EFP92676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337095.1">
        <property type="nucleotide sequence ID" value="XM_003337047.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L801"/>
    <dbReference type="GeneID" id="10539006"/>
    <dbReference type="KEGG" id="pgr:PGTG_18191"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18191"/>
    <dbReference type="eggNOG" id="ENOG502SE42">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053747_0_0_1"/>
    <dbReference type="InParanoid" id="E3L801"/>
    <dbReference type="OMA" id="MRIMADY"/>
    <dbReference type="OrthoDB" id="1600564at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001087">
        <property type="entry name" value="GDSL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22835">
        <property type="entry name" value="ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00657">
        <property type="entry name" value="Lipase_GDSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174092" description="SGNH hydrolase-type esterase domain-containing protein" evidence="2">
        <location>
            <begin position="26"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP92676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="36974" checksum="BB74798CD715D09B" modified="2011-01-11" version="1">MLLQANTPMLSVFVILCLTFISSRCDDQSNQRGEIPRSANQIKMAVQPQCGTLSSDKFVNFNTGINLDQIKTIYSFGDSWTSNGNYKGGVPQPAVPKGTEPHYGGRACNGPMWTEDLASGGKTLKSYAVGGATIDRTVWKARATKTDMVGHVDTFLSQKITVEPESSLATIFYGLNDYSASLQGPGSFEEAVKQLFNQTERLISAGLTQFIVVSPPYTHEIVKQFDSSIWNGFKKLKESKNIEFAYVDLVALFDAIHADLNSFGYKSDQACLKSPDTTVGGCENPNEYLYWIPSHPQYQTQQLMADWVRTVLEKCHQPNSNRRKRANSSKLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3L833</accession>
    <name>E3L833_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18223</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178370">
        <property type="protein sequence ID" value="EFP92708.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337127.2">
        <property type="nucleotide sequence ID" value="XM_003337079.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L833"/>
    <dbReference type="GeneID" id="10539206"/>
    <dbReference type="KEGG" id="pgr:PGTG_18223"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18223"/>
    <dbReference type="HOGENOM" id="CLU_888855_0_0_1"/>
    <dbReference type="InParanoid" id="E3L833"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="182"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="195"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="247"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92708.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="313" mass="35765" checksum="FB4789C9FF4C851D" modified="2012-04-18" version="2">MADTTNPTGPTGSATAQNVATEVGIIRQPRLSPTPSESPGRREETSDPNPDPNQRGHTPNTTGRRDSVNSRESTGLEIITETHANNPTNDEVFHRFPIEERFERYYYRMAENRAWLDQVNNQSTEEAIRAHLNYFRDDSVTRPRQRINQDSRVGSQLDPSIPLLKPDSKSPSKGQYPERITSGENCRQQQKYKQEPQPSEPTEPSHQKQSSASRGREHTSSSKPPSRETPYYERPASPQTGSRRKSREIPRTDSYEGSKEESRMENFNLLRDGQNRPFPPRNVSTSRGRKNSYSKRQSECASREPSRQPTRQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3L9Q6</accession>
    <name>E3L9Q6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19328</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178387">
        <property type="protein sequence ID" value="EFP93281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337700.1">
        <property type="nucleotide sequence ID" value="XM_003337652.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L9Q6"/>
    <dbReference type="GeneID" id="10543513"/>
    <dbReference type="KEGG" id="pgr:PGTG_19328"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19328"/>
    <dbReference type="eggNOG" id="ENOG502S7P8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_060582_0_0_1"/>
    <dbReference type="InParanoid" id="E3L9Q6"/>
    <dbReference type="OMA" id="ARAEAYW"/>
    <dbReference type="OrthoDB" id="2506877at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="323" mass="35602" checksum="91E72FC5ABFB1EB0" modified="2011-01-11" version="1">MLDEGDQKEFVRKMDWPFKLIVFGEENTLVSRGFFSNSHYWGNVLRHANGLLGVWSHNDQLNAGRAHMVNAIPGSISGAQEHTSWLFYSRCWTPEEAAFVGKSTAQLLKDNPRLSTQIPFTRMDTLLKVSHNGEVPVPSISITQEVKDGNIQDSGGSFQEQPLKIRTRRPANSAKLIAPTTLAQAPEITPDTSLAAGLGETLDTSNSDWASAKRSCLSKTGTSLATPGAVLDNPPSKRIRPNKVLPISAPEQAPKVGNVPAKCGRPKKARKVKTSITFKPEPLEDADWERIQARAAAYWRDKWEANPMMKDSNSARGFKKSID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3L9T0</accession>
    <name>E3L9T0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Domain of unknown function at the cortex 1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178388">
        <property type="protein sequence ID" value="EFP93305.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337724.2">
        <property type="nucleotide sequence ID" value="XM_003337676.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L9T0"/>
    <dbReference type="STRING" id="418459.E3L9T0"/>
    <dbReference type="GeneID" id="10543537"/>
    <dbReference type="KEGG" id="pgr:PGTG_19073"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19073"/>
    <dbReference type="eggNOG" id="ENOG502RXNE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047849_1_1_1"/>
    <dbReference type="InParanoid" id="E3L9T0"/>
    <dbReference type="OrthoDB" id="2119945at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013897">
        <property type="entry name" value="Duc1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34826">
        <property type="entry name" value="UPF0590 PROTEIN C409.17C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34826:SF2">
        <property type="entry name" value="UPF0590 PROTEIN C409.17C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08588">
        <property type="entry name" value="Duc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Domain of unknown function at the cortex 1" evidence="1">
        <location>
            <begin position="9"/>
            <end position="263"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93305.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="272" mass="30936" checksum="62431A5BF2A7A0F1" modified="2012-04-18" version="2">MALGHPSPKLQVLAGPSADELSPVNVNADKTDPFRIHTDRFEGALTVRIKNFLGADDCLSKETENKYFEEWPEMTCSIQIQGRFLQPTNADDCMWGNSFDRPIRDRLPYGTSVALKAISYIDPSLEHDIYSDKPWAWSPLLATMNHVQTERLESGDSPLPDWEGTRPVEDCNSVVGELETITSKRERRRFLSSPENRQACILGPRINVEFVNGFVDYSTLRLQIPIVKLSFRLDKLWDGQPVRYECISRSTLQTYFVIVVQIVELNGEPVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3LA95</accession>
    <name>E3LA95_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Helicase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_19382</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178394">
        <property type="protein sequence ID" value="EFP93470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337889.1">
        <property type="nucleotide sequence ID" value="XM_003337841.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LA95"/>
    <dbReference type="FunCoup" id="E3LA95">
        <property type="interactions" value="327"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3LA95"/>
    <dbReference type="GeneID" id="10543913"/>
    <dbReference type="KEGG" id="pgr:PGTG_19382"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19382"/>
    <dbReference type="HOGENOM" id="CLU_010647_2_0_1"/>
    <dbReference type="InParanoid" id="E3LA95"/>
    <dbReference type="OMA" id="WAHAHAH"/>
    <dbReference type="OrthoDB" id="6692397at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045025">
        <property type="term" value="C:mitochondrial degradosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000965">
        <property type="term" value="P:mitochondrial RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18805">
        <property type="entry name" value="SF2_C_suv3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002434">
        <property type="entry name" value="ATP-dependent RNA helicase SUV3, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000957">
        <property type="entry name" value="ATP-dependent RNA helicase SUV3L, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055206">
        <property type="entry name" value="DEXQc_SUV3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050699">
        <property type="entry name" value="RNA-DNA_Helicase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022192">
        <property type="entry name" value="SUV3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041082">
        <property type="entry name" value="Suv3_C_1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131">
        <property type="entry name" value="ATP-DEPENDENT RNA AND DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12131:SF1">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE SUPV3L1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22527">
        <property type="entry name" value="DEXQc_Suv3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12513">
        <property type="entry name" value="SUV3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18147">
        <property type="entry name" value="Suv3_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="80"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="203"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="355"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="212"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="355"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP93470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="439" mass="48292" checksum="4C76DE4F37010D28" modified="2011-01-11" version="1">MLSCQQFYDVVVIDEIQMIGDHFRGDAWTQAVLGVQAKELHLCGEESVVGLIESLANSCQDEFILHRYQRLTPLKVADSSLKGDLSKVQRGDCLVTFSRNNIYALKKAIQSATDLRVGMAYGGLPPEVREREAQMFNLGSQVEGEGGYDVLVGSDAIGMGLNLKIKRVIFQSLHKFDGRNEVALSTSQIKLIGGRAGRFGILPKNVGPGESGESREEGSTVGRRVDDERDGYESLTPIDGAPFEKIERAVLKAPFTTVEGLARRALPGSLTLTRPEYAIGDEKNSGSIADALQHILPLSLSERDLFCSAPASARSPVAISALQQWANAHALRRQVDFLAWLRHENVEQTIGSIESSINSSSSSSSSSATTTTTNHPKQNLIKLERLHNENLLRLESIHKCLVLYLWLAFRLPESFVDFERCQELKLRTETVLGIGLNSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3LAF6</accession>
    <name>E3LAF6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18945</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178396">
        <property type="protein sequence ID" value="EFP93531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337950.1">
        <property type="nucleotide sequence ID" value="XM_003337902.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LAF6"/>
    <dbReference type="GeneID" id="10543646"/>
    <dbReference type="KEGG" id="pgr:PGTG_18945"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18945"/>
    <dbReference type="HOGENOM" id="CLU_1462009_0_0_1"/>
    <dbReference type="InParanoid" id="E3LAF6"/>
    <dbReference type="OrthoDB" id="2510384at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="182" mass="21192" checksum="C5D90E7C6F6185AC" modified="2011-01-11" version="1">MATNQLTQTSHTGGDSTSTMEKTKLKEVQEQLTQLATRLDDLSAKHPPTLTLSVDRITCFDQFYPNLKPITKKMLEDLSFEDRMIMCSSTIERNEFYQEAAQSLRLYESLVAKAILQHQRPDNYLDIDQYIATILKKIDCDHQYKKLRSELLRLRLRRYRIESIIHRMETSVVVIAKPPTSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3LB74</accession>
    <name>E3LB74_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19844</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178409">
        <property type="protein sequence ID" value="EFP93799.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338218.2">
        <property type="nucleotide sequence ID" value="XM_003338170.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LB74"/>
    <dbReference type="GeneID" id="10535249"/>
    <dbReference type="KEGG" id="pgr:PGTG_19844"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19844"/>
    <dbReference type="HOGENOM" id="CLU_059608_0_0_1"/>
    <dbReference type="InParanoid" id="E3LB74"/>
    <dbReference type="OrthoDB" id="3241054at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021476">
        <property type="entry name" value="Egh16-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34618:SF1">
        <property type="entry name" value="SECRETED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34618">
        <property type="entry name" value="SURFACE PROTEIN MAS1, PUTATIVE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11327">
        <property type="entry name" value="Egh16-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174536" evidence="1">
        <location>
            <begin position="27"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93799.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="253" mass="25979" checksum="EB6657097CFAA4C4" modified="2012-04-18" version="2">MSIRSFTAISLAVACILVCLLPETQAGVCSILQVRGANGKIGTGFGVQPDNVVPRSGGGDTGADATEFQATPNPNPVCGTVAKLPEKRVDISSFMSQAIDAGVPSVFSNGSIPMTVFQVNRIGGGPMSCEYSSDGTGNSWQPMDMTLNMLGNFGIESQENTKVTVVTTLRAGSRFTGGPNHDIGLVRCRAGTDGICGGCFAVQMSGQVTDASNGGASATPATPTPLQLTSQQMSKVVSKVIDLAKTQNLVATN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3LBS4</accession>
    <name>E3LBS4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178425">
        <property type="protein sequence ID" value="EFP93999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338418.1">
        <property type="nucleotide sequence ID" value="XM_003338370.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LBS4"/>
    <dbReference type="GeneID" id="10535612"/>
    <dbReference type="KEGG" id="pgr:PGTG_20015"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20015"/>
    <dbReference type="HOGENOM" id="CLU_1960655_0_0_1"/>
    <dbReference type="InParanoid" id="E3LBS4"/>
    <dbReference type="OrthoDB" id="10420110at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="128" mass="14769" checksum="50C38D10D7903899" modified="2011-01-11" version="1">MIKRFEFEKRPREAMQCKSYQPPNVPKHSRKPKPNVRYQTPPHSFESDHPFIEAVEHNEPGLNEPADGRDVYDESGLGDNLNEEYRSDCNVLDDECPSNLDEHVDDLPEEEQASETVSACYYGRDNLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3LC17</accession>
    <name>E3LC17_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_20079</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SNAP-25 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178439">
        <property type="protein sequence ID" value="EFP94092.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338511.2">
        <property type="nucleotide sequence ID" value="XM_003338463.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LC17"/>
    <dbReference type="FunCoup" id="E3LC17">
        <property type="interactions" value="25"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3LC17"/>
    <dbReference type="GeneID" id="10535784"/>
    <dbReference type="KEGG" id="pgr:PGTG_20079"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20079"/>
    <dbReference type="HOGENOM" id="CLU_020823_0_1_1"/>
    <dbReference type="InParanoid" id="E3LC17"/>
    <dbReference type="OrthoDB" id="18679at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd15886">
        <property type="entry name" value="SNARE_SEC9N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305">
        <property type="entry name" value="SYNAPTOSOMAL ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19305:SF9">
        <property type="entry name" value="SYNAPTOSOMAL-ASSOCIATED PROTEIN 29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="132"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="132"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP94092.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="275" mass="31108" checksum="96477CE5E0A725B5" modified="2012-04-18" version="2">MNNKRKSMVHLHLSLNQAPPESDESKSMVGFRNPDLQFGTTQKTGVLLEVRPAGLGHEELFSNTSGNQLDDEDEEVEAIKQQIKFAKQESLSSTRNALRIAREAEGTARNTMNRLGEQSDRLANTENHLDLAKAHAKGKRDAHEQKLTSRHEEEKAEDRRNLEGFRQGGPNQPKGALGGLRSRLADKARCQFENTESDDELEDELDNNLDEISGLTSRLMGTEIDSQNHHLALIEFVVLTYTALDTFGLVYRFRASFILSQLIVRRGKREWREAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3LC37</accession>
    <name>E3LC37_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_20149</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178442">
        <property type="protein sequence ID" value="EFP94112.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338531.2">
        <property type="nucleotide sequence ID" value="XM_003338483.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LC37"/>
    <dbReference type="GeneID" id="10535802"/>
    <dbReference type="KEGG" id="pgr:PGTG_20149"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20149"/>
    <dbReference type="HOGENOM" id="CLU_004591_4_2_1"/>
    <dbReference type="InParanoid" id="E3LC37"/>
    <dbReference type="OrthoDB" id="10300969at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP94112.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="209" mass="24496" checksum="797B1F85202717C8" modified="2012-04-18" version="2">MAYLCVLCMGKTTTNRNRHCHNDHHQILVRAEKGWVERIASWRAENQASGKFNYVVDEGIIQMDMVVDQNQETCVEGFELEPDENELVPEPYIVPLDIFEEEESKINESNEDPLWMSFNQDSVGQISSEPDVLSSIDPLPLFRIEEEKIDNATADNLTWFPFLNKEYLIASLLVGYLHKLISRHLYHQFQLVFTLCHIRLPRWETVRRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3NXI0</accession>
    <name>E3NXI0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_20227</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS989941">
        <property type="protein sequence ID" value="EFP94279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338698.1">
        <property type="nucleotide sequence ID" value="XM_003338650.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NXI0"/>
    <dbReference type="GeneID" id="10527413"/>
    <dbReference type="KEGG" id="pgr:PGTG_20227"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20227"/>
    <dbReference type="HOGENOM" id="CLU_1462002_0_0_1"/>
    <dbReference type="InParanoid" id="E3NXI0"/>
    <dbReference type="OrthoDB" id="10282103at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP94279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="153" mass="17270" checksum="01F23BA2013E4512" modified="2011-01-11" version="1">MALLPVRGGDTYVPYASRLWTPNLPSYTITAKPITLASRSPGPYLTYYGTISSFPLDGPIEVHATTSYWESECPPFMRHGGSPQVHGNFRILSHDHRLLMSFRIKCPGFLALYSDVLAPDHLIWIKGRILRRTSIPAPCVHIREAYWNNQSNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPF6</accession>
    <name>H6QPF6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_20886</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EHS63904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890599.1">
        <property type="nucleotide sequence ID" value="XM_003890550.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPF6"/>
    <dbReference type="GeneID" id="13543187"/>
    <dbReference type="KEGG" id="pgr:PGTG_20886"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20886"/>
    <dbReference type="HOGENOM" id="CLU_2923744_0_0_1"/>
    <dbReference type="InParanoid" id="H6QPF6"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="61" mass="7089" checksum="C7612EEE0FF488C9" modified="2012-04-18" version="1">MSGVNSIQMIPECEVEFLEISCEPSVTLLMDRMMEDEDERMELFEDEDSEYNEAGFLGAFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPK5</accession>
    <name>H6QPK5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_20872</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EHS63890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890585.1">
        <property type="nucleotide sequence ID" value="XM_003890536.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPK5"/>
    <dbReference type="GeneID" id="13543194"/>
    <dbReference type="KEGG" id="pgr:PGTG_20872"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20872"/>
    <dbReference type="HOGENOM" id="CLU_3015253_0_0_1"/>
    <dbReference type="InParanoid" id="H6QPK5"/>
    <dbReference type="OrthoDB" id="10273020at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="56" mass="5926" checksum="B670CFA0AEECE120" modified="2012-04-18" version="1">MPLSMIFGRASMLRVPLHTNPDTGHLDTRIIKGAVEAAAACTNLTRLDASGSDDDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQ36</accession>
    <name>H6QQ36_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21015</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EHS64664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890370.1">
        <property type="nucleotide sequence ID" value="XM_003890321.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQ36"/>
    <dbReference type="STRING" id="418459.H6QQ36"/>
    <dbReference type="GeneID" id="13542786"/>
    <dbReference type="KEGG" id="pgr:PGTG_21015"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21015"/>
    <dbReference type="eggNOG" id="ENOG502QR5Z">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1200334_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQ36"/>
    <dbReference type="OrthoDB" id="2287304at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47150:SF5">
        <property type="entry name" value="OS07G0546750 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47150">
        <property type="entry name" value="OS12G0169200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="231" mass="26240" checksum="3084299954A1EA96" modified="2012-04-18" version="1">MFYIRASPAKEVNRVAKPARIATQLGPLATRAALPNTQGCYYNYQRSVNLHYPSSPGIASTSPQYSSIKRHITPPHTPQTTMNTQFLENLLESQAIALATTRQVQTVLSDTVNVILNQDNEESNQETLVRGGSRPGRRANLNHGFEEGYQRFYKDYLAPEPVYVDDLFRRQFRMHRGLFLKIVDDLTEHNHYFKRKADALGRLGLYPVQKVASAIWMLAYGGAADINDEYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQ48</accession>
    <name>H6QQ48_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EHS64649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890355.1">
        <property type="nucleotide sequence ID" value="XM_003890306.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQ48"/>
    <dbReference type="GeneID" id="13542746"/>
    <dbReference type="KEGG" id="pgr:PGTG_21000"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21000"/>
    <dbReference type="HOGENOM" id="CLU_1107571_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQ48"/>
    <dbReference type="OrthoDB" id="2414509at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="251" mass="28240" checksum="BDADE2AD3FD052DC" modified="2012-04-18" version="1">MSQEISEVRPDLPDLTIPAALNQAADSSTTPKPKRNRRTNAEMEIYRASQEIEKSTSKKTNNEKEFKYWVPTARRLAALKKNSNAHRSAAKPATSTSRTADANPQFIATDYQLICSYLEDPQHYTQIYGNGTRTSVGPQSLTKGAAYDVFAIYMNDQTNKRLHLDGKLLRQRIEAYKKRFTNAKHWADTTGAGIDICDNGITIKAKLESMCPCYERMDQIFGGKPNVTPAAQYESQGGMELYDQPMDDDRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>H6QS12</accession>
    <name>H6QS12_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21611</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EHS63483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    <dbReference type="GeneID" id="13542823"/>
    <dbReference type="KEGG" id="pgr:PGTG_21611"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21611"/>
    <dbReference type="HOGENOM" id="CLU_085453_0_0_1"/>
    <dbReference type="InParanoid" id="H6QS12"/>
    <dbReference type="OrthoDB" id="10465936at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="203" mass="23679" checksum="C9BF4FEEE323FB08" modified="2012-04-18" version="1">MAREHRQQIEEENEPPIQIEAVESDNADEEEENTTHNINEILHAMGLTANGPGSTESSRRNLHENIRLDDLINAHLEDLFRHDDETVFESDEPADPEDAIDDPDEQSRWFPFKNKMELVGSLLIGHTHSRISREIYNKFRAVITICDTRLPAWSTVRSARERIRRVLERSGEFYPRNGSRSWLHNIVLQCVKWKENITIFLNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSM8</accession>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_21730</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EHS63764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889467.1">
        <property type="nucleotide sequence ID" value="XM_003889418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSM8"/>
    <dbReference type="GeneID" id="13541926"/>
    <dbReference type="KEGG" id="pgr:PGTG_21730"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21730"/>
    <dbReference type="HOGENOM" id="CLU_045017_0_0_1"/>
    <dbReference type="InParanoid" id="H6QSM8"/>
    <dbReference type="OrthoDB" id="10288202at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003606553" evidence="2">
        <location>
            <begin position="24"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="60"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="94"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS63764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="486" mass="54226" checksum="F7D94EE767574508" modified="2012-04-18" version="1">MTRIRLTFLGLVLCYSNVVITMAGEGMTSLSHPPAHDMDQWMSDHLDDITDCNLLVPSNKPRLSDEHTHSINLDSDNPNSNEFIEGYSSDLLHSPESPGSDSSSSYSVGSEFRDKDMHEAVNSYQPSLPMVKEGGQAQELKVADQQADQHESLSSVKSKPRSQRQTGSDSAPATWGSRGQLMEGPQPDQGQELESPDNLENDVVLLQKAQRATRKRKTPVQASTGVPYSAMDDGKLMDKLVIYARKLSESQSAEDREAFRIVQAQIAAKRETLIKLQRLQVTDASRVKLENKIVQEPDSELTEREIFIFPNPPPDSDRVSIRLLRDNRTKGAIYVGERLVTRVDKVAQFLDIFDHLFSRLALNNNERGIRPAQAGDHREVLDRFYNLIFTETMEHPPLMGSAKRSSLAGEQPKKFSPAQMSLYKVLTQRPELTNAQAAAVALKFRQEFDKPMSPIGAMPLHELVAKASSLDPSLEPVKKVKKEPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTE3</accession>
    <name>H6QTE3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21894</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178311">
        <property type="protein sequence ID" value="EHS64144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889224.1">
        <property type="nucleotide sequence ID" value="XM_003889175.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTE3"/>
    <dbReference type="GeneID" id="13541694"/>
    <dbReference type="KEGG" id="pgr:PGTG_21894"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21894"/>
    <dbReference type="HOGENOM" id="CLU_3051439_0_0_1"/>
    <dbReference type="InParanoid" id="H6QTE3"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="54" mass="5900" checksum="8954BF9CA46874F4" modified="2012-04-18" version="1">MAMLVQFSLLSIDAYGIGSEGKMLTWFKKAFEAEASPFCSQVASRTQAQVDPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTI7</accession>
    <name>H6QTI7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22057</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178314">
        <property type="protein sequence ID" value="EHS64202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889190.1">
        <property type="nucleotide sequence ID" value="XM_003889141.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTI7"/>
    <dbReference type="GeneID" id="13541218"/>
    <dbReference type="KEGG" id="pgr:PGTG_22057"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22057"/>
    <dbReference type="HOGENOM" id="CLU_2110124_0_0_1"/>
    <dbReference type="InParanoid" id="H6QTI7"/>
    <dbReference type="OrthoDB" id="2504957at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="97" mass="11305" checksum="6AD9D65D23D53175" modified="2012-04-18" version="1">MEAVVKKNKTLVNYFTNSGFWRKHLTMWQKENGVKNGLQTLCETWWYSMGKVCLGVQLHEIGFQKCLELLQNQQVDTPLMTDAVVKVIEDRDHFTAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTY1</accession>
    <name>H6QTY1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22226</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178324">
        <property type="protein sequence ID" value="EHS64395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889040.1">
        <property type="nucleotide sequence ID" value="XM_003888991.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTY1"/>
    <dbReference type="STRING" id="418459.H6QTY1"/>
    <dbReference type="GeneID" id="13541832"/>
    <dbReference type="KEGG" id="pgr:PGTG_22226"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22226"/>
    <dbReference type="HOGENOM" id="CLU_1272824_0_0_1"/>
    <dbReference type="InParanoid" id="H6QTY1"/>
    <dbReference type="OrthoDB" id="4605274at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053228">
        <property type="entry name" value="Stereospecific_Lipase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37574">
        <property type="entry name" value="LIPASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37574:SF1">
        <property type="entry name" value="LIPASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="217" mass="23414" checksum="7C49BEA0EBC1CB74" modified="2012-04-18" version="1">MSATRHPSDAIYGFYPSGNVVPLPLVKRQSAQAGIVSDRDHFYDVAAAPTGPLPSRASGDAPWTQAESSYKRMIACPFGIQNKDRGIVLLVPGTAGNASEVYKSVRLPYKVEFPSICSTVPSIFALKTIQSAYYADLPSQGFDVCWVDTPNYSISDMQLSAEFVAYAIKSLAPQSTKTGGKINIVSYSQGGPNVQWASSLLDFLDVFFKLAGQGDRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTY6</accession>
    <name>H6QTY6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22197</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178325">
        <property type="protein sequence ID" value="EHS64400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889024.1">
        <property type="nucleotide sequence ID" value="XM_003888975.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTY6"/>
    <dbReference type="GeneID" id="13540963"/>
    <dbReference type="KEGG" id="pgr:PGTG_22197"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22197"/>
    <dbReference type="HOGENOM" id="CLU_2147099_0_0_1"/>
    <dbReference type="InParanoid" id="H6QTY6"/>
    <dbReference type="OrthoDB" id="10268255at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="112" mass="12738" checksum="F9BBFAF7D87D72A8" modified="2012-04-18" version="1">MEDPHQRILDEAEVNVHRRGIDVSNKPTCSTSARMWDCGTNVEKKVGSWNRRRDRLLPKLYRADLVGSTEKPSFVGSTTPRGTNCPWGVPQGSAQTWMISEGGVRICRYWYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>H6QU31</accession>
    <name>H6QU31_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ER membrane protein SH3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_22269</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178328">
        <property type="protein sequence ID" value="EHS64456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888983.1">
        <property type="nucleotide sequence ID" value="XM_003888934.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QU31"/>
    <dbReference type="STRING" id="418459.H6QU31"/>
    <dbReference type="GeneID" id="13541079"/>
    <dbReference type="KEGG" id="pgr:PGTG_22269"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22269"/>
    <dbReference type="eggNOG" id="ENOG502S73S">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1050008_0_0_1"/>
    <dbReference type="InParanoid" id="H6QU31"/>
    <dbReference type="OrthoDB" id="5229808at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013248">
        <property type="entry name" value="Psh3/Shr3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28228">
        <property type="entry name" value="SECRETORY COMPONENT PROTEIN SHR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28228:SF1">
        <property type="entry name" value="SECRETORY COMPONENT PROTEIN SHR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08229">
        <property type="entry name" value="SHR3_chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00786">
        <property type="entry name" value="SHR3_chaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="81"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="223"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS64456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="277" mass="30103" checksum="739E90FA96CB3563" modified="2012-04-18" version="1">MSSSTTSPGQPEQPKTTPPSPSPSALPTLTVFDGVSNALLLSSIGFLLGALFMALTTDYALLYTHAPTTAEAYLAAEQAYLTLWTSPLAVKAIFHLLLLLPIFTLSVKVSRYTEAAIYFDGLCLGMMLLVLGLYTGSTVPNLRKLAFEPSTQALADLVKNSLNLKIPTDFDPLSPTSVINRLLVLLSHPASILLKPQKALESEQEFKKALELNPLDEKSRHELLAVTAAGHSIAIFLLIGIIVLQIGKVYAEVEDAKLKARFELEESNRVAQDRKDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUD5</accession>
    <name>H6QUD5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Transcription factor IIIC 90kDa subunit N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_22386</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178336">
        <property type="protein sequence ID" value="EHS64598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888878.1">
        <property type="nucleotide sequence ID" value="XM_003888829.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUD5"/>
    <dbReference type="STRING" id="418459.H6QUD5"/>
    <dbReference type="GeneID" id="13542404"/>
    <dbReference type="KEGG" id="pgr:PGTG_22386"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22386"/>
    <dbReference type="eggNOG" id="ENOG502S5N8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_291722_0_0_1"/>
    <dbReference type="InParanoid" id="H6QUD5"/>
    <dbReference type="OrthoDB" id="421374at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000127">
        <property type="term" value="C:transcription factor TFIIIC complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006384">
        <property type="term" value="P:transcription initiation at RNA polymerase III promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044230">
        <property type="entry name" value="GTF3C4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024761">
        <property type="entry name" value="TFIIIC_delta_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024764">
        <property type="entry name" value="TFIIIC_Znf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15496:SF2">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15496">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR 3C POLYPEPTIDE 4 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12657">
        <property type="entry name" value="TFIIIC_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12660">
        <property type="entry name" value="zf-TFIIIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor IIIC 90kDa subunit N-terminal" evidence="2">
        <location>
            <begin position="81"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor IIIC putative zinc-finger" evidence="3">
        <location>
            <begin position="922"/>
            <end position="1041"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="112"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="973"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS64598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1046" mass="115574" checksum="56B625B3CD798B22" modified="2012-04-18" version="1">MYARCRLSEFCDEDRLSVGRGGDVHETEPERYKDTDDMSSDTAAGQTTQPIDLPKPTDSSYGHADILQTLPIDSSPSCLEWSNDGQAFAVTKANIYLLTPILGYLVSPEDRHTHTQAQPTENGSHSQDAPSTPSRSNPSGIQESIADSTTKSAGGQPKIPFFTTIIEINKQLGVNWSTHSNDISTITPPNDDRFWRAATWSPSGLSALGSCLLAGLSTTCDVFVYSPCQNYQAGLWAIKETLNLSEELLTIFYDFYPSIIGHQEDPTKSIDVSPETAWDISEEGEEKRSRFTAGVLRTQATCIAWSPAYSHSNCDTPVLNQHDDLYDVDFSLLAVGHRRGDLSLWRHTSKGHMELESFNPICSDGRTLNLLSWSDWKLSARRRVDDTSAQQYQLTAHLAVANSKGVVYLMQVCRPFERPTKPVSPISRMEIQIIGIYQDPLNQSSITYFKWLPPSGNTLSRLIISRLGEIVLVPLSSTSTEGQSDSQSGLFLGPTQVIQLPVLQPHDDRLCWADCNSWATCSGMSIIPTSLPGITQIIAMLSNGLIFVLRESLDPNPSSSSSAMANPLELDLAHSVQLSLDFRAKFRAIGYSAHPPPNSTPITKQNVMSVYGSHVLSGLQHVSGDHQQLHSIDPISLLSSCIMSWLYEIDAPNKFRYKPENHQILQFCLAYFNPVGSVQRRVQLLGMLENQITAILPAMLSKSASSILVSPSSKLLNIFNILHSIFSDPGSRESPSFDLLNSTLSNLVDLLPLGTETAYFALPDHQPILNYDGGTTSMALKSQLINQLFYNLNLDHLRLKVNLCNFLLGRADKAKFPSLRSRLVEAKVSLSRVIHRLVLQTIAQFFLRHVGQLSSDERPVFNRYQCATKAIDRFPEPTIEQLLEPDEVDHKLLDQDRLSSAPDDSLGNGNPLISFGGEENEVEQCPACLQSVCFDSLRFAICRVGHVWDRCSVTFQILSTIKVRICTGCGRKSMVKGGGEDQTTSRPPEASQEGAQMETGDHQHPSTSTQQEDADVGRLGKPSLVQVLLDSSICCWHCGGRWRLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQN9</accession>
    <name>E3JQN9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00644</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307694.1">
        <property type="nucleotide sequence ID" value="XM_003307646.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQN9"/>
    <dbReference type="GeneID" id="10527849"/>
    <dbReference type="KEGG" id="pgr:PGTG_00644"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00644"/>
    <dbReference type="HOGENOM" id="CLU_045924_0_0_1"/>
    <dbReference type="InParanoid" id="E3JQN9"/>
    <dbReference type="OrthoDB" id="10284337at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="215"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="282"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP74688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="409" mass="44715" checksum="757D3C20C41222F9" modified="2011-01-11" version="1">MSDTGQHQETEAITPAIMAVGPRIRYPIIYLSYQVWVEHRVLFPNGTPSYARRSEEPRTPDNWKYHHECITTNDEDVVPIPPCEVPFQHMAFRDFRRCVIEAVGATRSDFDFTRVLRAADAANKLQWRGYRTCPGGPTPRNMNVVRNYANFAQMATLAALPMRCHLRLFMDDPAQIIGNRSYWTWIPHIWPHEVIVPFVRNGALPPPPEECFVLGAPLPGSDAGEEATSTRSSVEPPPLPLATSPTPDPTGTRSTVEPPPSPSSWVINTPSSTTPTPPLVESPSYHSSSARSSSVHEWPASSDFNSDGPAVDPPLSSPRQDNGVKAPSSGQRSLVAAEGVISGPSSSNIRGSRVARDDPPSSSGIEMLDGPPSSWKSKDYETIKQVRSSSPDVEVVSKKSGSLKACRST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQZ5</accession>
    <name>E3JQZ5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Homeobox domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_00378</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TALE/M-ATYP homeobox family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EHS62493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307428.1">
        <property type="nucleotide sequence ID" value="XM_003307380.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890810.1">
        <property type="nucleotide sequence ID" value="XM_003890761.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQZ5"/>
    <dbReference type="SMR" id="E3JQZ5"/>
    <dbReference type="STRING" id="418459.E3JQZ5"/>
    <dbReference type="GeneID" id="10527523"/>
    <dbReference type="KEGG" id="pgr:PGTG_00378"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00378"/>
    <dbReference type="eggNOG" id="KOG0773">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_402319_0_0_1"/>
    <dbReference type="InParanoid" id="E3JQZ5"/>
    <dbReference type="OMA" id="HANLEMC"/>
    <dbReference type="OrthoDB" id="10056939at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00086">
        <property type="entry name" value="homeodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR11850">
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    </dbReference>
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        <property type="entry name" value="Homeobox_KN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="HOX"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50071">
        <property type="entry name" value="HOMEOBOX_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0371">Homeobox</keyword>
    <keyword evidence="4 5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Homeobox" evidence="7">
        <location>
            <begin position="392"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="DNA-binding region" description="Homeobox" evidence="5">
        <location>
            <begin position="394"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="93"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="163"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="238"/>
            <end position="257"/>
        </location>
    </feature>
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        <location>
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        </location>
    </feature>
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        <location>
            <begin position="559"/>
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        </location>
    </feature>
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        <location>
            <begin position="106"/>
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        </location>
    </feature>
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        <location>
            <begin position="165"/>
            <end position="174"/>
        </location>
    </feature>
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        <location>
            <begin position="559"/>
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        </location>
    </feature>
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        <location>
            <begin position="602"/>
            <end position="618"/>
        </location>
    </feature>
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        <location>
            <begin position="620"/>
            <end position="634"/>
        </location>
    </feature>
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        <location>
            <begin position="644"/>
            <end position="654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="675"/>
            <end position="684"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023155"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP74422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="684" mass="73523" checksum="D0C19DC9F1BD316D" modified="2011-01-11" version="1">MLASPLPSTDVTSDQSFSEVALIEQQHLTTAAPRRTSYSLYYHNNNEPEVYPEFNWENHSSIQLPNMGDPSRSESNAAEGKLHNSPLASALEPYQGAAPASPPSSRSETFNSDGGNVNLEICAAPQVPQDDSQAQEPLRVSNKRPRPPCLSQLDSRCFERYPQPHLTSANSSPRSAAEASLKKESEDLSSLPPIVTREEIERGVVSNSNKSVLHVPTAHLERHKHDLVAIDALGNSSVRRTPEANSNPHPQQLQFSSHSNGYLSALPSPVLPSCNGTNPGDRVSYPASPVVSLTAPNSATFPLESNSSFSPLTTASQANQVVSSPSNHHHVSDTAFSQTALPRLIRPSYASNGGTQVVDSSVAPTTGAPATRVTVSPAPQPAQASPWSSVETQAPRRRGKLPSAVTAILKGWLMAHTTHPYPTEEEKKSLCQETNLTMNQVSNWFINARRRILVPPSAGNSVHEVRQPVRRQAQSQLVRAAGHAGAVPPCLTIRHIGPQSVPASPSTSISMFSPVSATSPNLSNGVGSFDFSYPLAHQSGQENHYHGEGLSACRPTNTLSPCSSQWPHSTQLMRQSPSSPLYPQPHGPTPYYSSSHSFQGYPPSHSSSSTPLPSPHFSASFPSGPTHFPNTQARLHSPRPNPDSHPSTPTLSHSSNEHHSTPAYMSLAPCHPSISYPTNQDGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3JSF9</accession>
    <name>E3JSF9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01525</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP74932.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319351.2">
        <property type="nucleotide sequence ID" value="XM_003319303.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JSF9"/>
    <dbReference type="GeneID" id="10547134"/>
    <dbReference type="KEGG" id="pgr:PGTG_01525"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01525"/>
    <dbReference type="HOGENOM" id="CLU_1949835_0_0_1"/>
    <dbReference type="InParanoid" id="E3JSF9"/>
    <dbReference type="OrthoDB" id="10322801at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="69"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP74932.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="129" mass="13161" checksum="F99A9EA0CF7395EB" modified="2012-04-18" version="2">MPNTCTADFSATKLYRKHQSNVPTHLLYSAPPQSYSLQAQHQPQTEKTSSHLRTVHQPSPGTIQSSGHVVSPSDPVTSVPAAANHQSGGSETGGGSPDFGSGVAGIVGAPTGLGTGLGAVELLFGMNRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3JU08</accession>
    <name>E3JU08_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00864</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75533.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319952.2">
        <property type="nucleotide sequence ID" value="XM_003319904.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JU08"/>
    <dbReference type="GeneID" id="10543249"/>
    <dbReference type="KEGG" id="pgr:PGTG_00864"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00864"/>
    <dbReference type="HOGENOM" id="CLU_1897237_0_0_1"/>
    <dbReference type="InParanoid" id="E3JU08"/>
    <dbReference type="OrthoDB" id="10281882at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75533.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="134" mass="14591" checksum="EC0060693890D3CD" modified="2012-04-18" version="2">MRAPDLGAVGAMQMQGWATPGHSHTQRPGKSCVDLCRVPLSGGYMAVPKPISHESPLTETQGRDSRRRQSACLVRGGEKPSLGDWPDSIECTDGEDLSRQLPPACNFDNRAVAPRETLTARKTMSHLFDMVADM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3JU25</accession>
    <name>E3JU25_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">sterol 3beta-glucosyltransferase</fullName>
            <ecNumber evidence="2">2.4.1.173</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Autophagy-related protein 26</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_00881</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>a sterol + UDP-alpha-D-glucose = a sterol 3-beta-D-glucoside + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22724"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15889"/>
            <dbReference type="ChEBI" id="CHEBI:37424"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.173"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:22725"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ergosterol + UDP-alpha-D-glucose = ergosteryl 3-beta-D-glucoside + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61836"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16933"/>
            <dbReference type="ChEBI" id="CHEBI:52973"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:61837"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 28 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.173" evidence="2"/>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319969.1">
        <property type="nucleotide sequence ID" value="XM_003319921.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JU25"/>
    <dbReference type="GeneID" id="10543266"/>
    <dbReference type="KEGG" id="pgr:PGTG_00881"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00881"/>
    <dbReference type="eggNOG" id="KOG1192">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000537_8_5_1"/>
    <dbReference type="InParanoid" id="E3JU25"/>
    <dbReference type="OMA" id="WIREHGI"/>
    <dbReference type="OrthoDB" id="10261837at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016906">
        <property type="term" value="F:sterol 3-beta-glucosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008194">
        <property type="term" value="F:UDP-glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016125">
        <property type="term" value="P:sterol metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03784">
        <property type="entry name" value="GT1_Gtf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000029">
        <property type="entry name" value="Sterol 3-beta-glucosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2000:FF:000009">
        <property type="entry name" value="Sterol 3-beta-glucosyltransferase UGT80A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010610">
        <property type="entry name" value="EryCIII-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050426">
        <property type="entry name" value="Glycosyltransferase_28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004276">
        <property type="entry name" value="GlycoTrans_28_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002213">
        <property type="entry name" value="UDP_glucos_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48050">
        <property type="entry name" value="STEROL 3-BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48050:SF25">
        <property type="entry name" value="STEROL 3-BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06722">
        <property type="entry name" value="EryCIII-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03033">
        <property type="entry name" value="Glyco_transf_28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase family 28 N-terminal" evidence="8">
        <location>
            <begin position="49"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Erythromycin biosynthesis protein CIII-like C-terminal" evidence="9">
        <location>
            <begin position="363"/>
            <end position="434"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF06722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP75550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="572" mass="64553" checksum="FBB6681D7C3CF354" modified="2011-01-11" version="1">MKRLSFPAIGSLIRLGILVAGISLTLQALGSPSKRPISFVAGASRPLKITCLTIGSRGDVQPFIALGLGLQQDGHIVTIATHLEFKDLIEDSGIGFRNIGGNPQELIKHCVEYGFFSPEFYIEGYTKFREWVDELLITVPAACQGTDVLIESPTAMMGIHVAESMKIPYFRAFTMPWTPTTEYPHPFAVTSQQLGKYYNRMSYTMFNYLIWKGIESKVNKWREKKLKLKPTSFAKLRTADIPYLYNFSEHIVPKPHDWSDKTHITGYWFRDQQKKSDQKKIEESIPLDLRSFLQKAKDNGKKVIYIGFGSVIFPNAEEVQRKLEKAVRKAGVWAVVSGGWSDMKPLEKDLESVGSSRSQDTPIHYVGAVPHEWLFSQVDATLTHGGAGTTAASLRAGIPTLIKPFFGDQFFWAKLVKKMGVGGHVKKFKVSELARAFRIATTNREQIWRAKKIGEAIRKENGVRTAIQKMYKDMDYARRLMMVGLGQDDYGPIPKFPREKVYSWLKQFLQDEKSSIHLLLLCILRALQYYSTQQQALGSNHQNLQPPLQATLAWNLTASIANQPPLSTLLTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUZ1</accession>
    <name>E3JUZ1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_01197</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320285.1">
        <property type="nucleotide sequence ID" value="XM_003320237.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JUZ1"/>
    <dbReference type="GeneID" id="10532781"/>
    <dbReference type="KEGG" id="pgr:PGTG_01197"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01197"/>
    <dbReference type="HOGENOM" id="CLU_2159663_0_0_1"/>
    <dbReference type="InParanoid" id="E3JUZ1"/>
    <dbReference type="OMA" id="QTVIMTK"/>
    <dbReference type="OrthoDB" id="2510054at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="2">
        <location>
            <begin position="13"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP75866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="111" mass="12254" checksum="18EF034D2A4F424B" modified="2011-01-11" version="1">MTEIDSDGVEQERKKKDRSKKRARSPSSNVPQSLPTSEPAGTADEGLVSTTTPADSESTDRPMCKKKAKALHRSKVDGSEDWKDKVAIAQKEIAAQAKRQNEYCGRPRPNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3JVX2</accession>
    <name>E3JVX2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02638</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003320568.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JVX2"/>
    <dbReference type="GeneID" id="10533765"/>
    <dbReference type="KEGG" id="pgr:PGTG_02638"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02638"/>
    <dbReference type="HOGENOM" id="CLU_1897241_0_0_1"/>
    <dbReference type="InParanoid" id="E3JVX2"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="134" mass="15639" checksum="9E7447BDCB42E369" modified="2011-01-11" version="1">MMIEKPVRQHHRAGRKRSKQERKKGNRAGQRTGSCGSRNSWPKYCPEQRWLVTLKCPQPSYVLTWEKKETEWIAQFVPQALPPLTGDWSGFISLPKCKQASVRHNQMAGKLTTRKLVQRDCLLYFGRRSLMLLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWJ5</accession>
    <name>E3JWJ5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_02861</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76420.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320839.2">
        <property type="nucleotide sequence ID" value="XM_003320791.2"/>
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    <dbReference type="AlphaFoldDB" id="E3JWJ5"/>
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    <dbReference type="KEGG" id="pgr:PGTG_02861"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02861"/>
    <dbReference type="HOGENOM" id="CLU_1846068_0_0_1"/>
    <dbReference type="InParanoid" id="E3JWJ5"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173021" description="Secreted protein" evidence="2">
        <location>
            <begin position="28"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP76420.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="151" mass="16475" checksum="8C28A23F1CC19383" modified="2012-04-18" version="2">MFRTVTHGSRILSLCFVLWALVAVVSSALSNPAELRRCPRCSKDTLNDIVWGTARDVPRCDKPIRCKEGHVTRQACGAPMSMYVKQCSNEVGKGRKKAPCGYVGDPFHTCGAPRFHSNPQACKCPRSVIESQSDYGSSSQSGRSSSSSFWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWK7</accession>
    <name>E3JWK7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Replication factor C subunit 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_02873</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76432.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320851.2">
        <property type="nucleotide sequence ID" value="XM_003320803.2"/>
    </dbReference>
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    <dbReference type="FunCoup" id="E3JWK7">
        <property type="interactions" value="678"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JWK7"/>
    <dbReference type="GeneID" id="10534487"/>
    <dbReference type="KEGG" id="pgr:PGTG_02873"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02873"/>
    <dbReference type="eggNOG" id="KOG0990">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042324_2_0_1"/>
    <dbReference type="InParanoid" id="E3JWK7"/>
    <dbReference type="OrthoDB" id="4199794at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="C:Elg1 RFC-like complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031389">
        <property type="term" value="C:Rad17 RFC-like complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902983">
        <property type="term" value="P:DNA strand elongation involved in mitotic DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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    <dbReference type="GO" id="GO:0006261">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070914">
        <property type="term" value="P:UV-damage excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
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    </dbReference>
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        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
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        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
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        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
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        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
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        <property type="entry name" value="Rep_factorC_C"/>
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    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001679">
        <property type="entry name" value="PRK00440.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF9">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
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        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
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    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="61"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00070184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP76432.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="352" mass="39653" checksum="72AFD751F9CA51A6" modified="2012-04-18" version="2">MDKGKGKEIGKQPEIAANGASKPASKEHLPWVEKYRPSTLDDVVSHQDIIQTIQKFISANQLPHLLFYGPPGTGKTSTILAVARQLFQTPMSFKNNILELNASDDRGIDVVREQIKNFASARMVFSSGFKLIILDEADQMTQAAQSALRRVIEQYTKNVRFCIICNYVNRINPAIQSRCTRFRFGPLDHPEIERRIAHVADAESVKISDDGRKALLNLCKGDMRRVLNVMQACHSGYGKVDEDAVYECTGSPHPKQIEKIVESMMNDEFQTSFKRIQDIKINYGLALQDIISGIYDYLRTIVFEKNAQIYILDQLSQIEHRLSTGATEKIQLSSLLGSFRIAVEITSRSQTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWW5</accession>
    <name>E3JWW5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02981</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320959.1">
        <property type="nucleotide sequence ID" value="XM_003320911.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JWW5"/>
    <dbReference type="GeneID" id="10528396"/>
    <dbReference type="KEGG" id="pgr:PGTG_02981"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02981"/>
    <dbReference type="HOGENOM" id="CLU_358680_0_0_1"/>
    <dbReference type="InParanoid" id="E3JWW5"/>
    <dbReference type="OMA" id="AQKCERF"/>
    <dbReference type="OrthoDB" id="2503257at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038023">
        <property type="term" value="F:signaling receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="15"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="340"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="427"/>
            <end position="473"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="569"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="131"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="360"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="379"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="441"/>
            <end position="451"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="840" mass="91212" checksum="092430B8DD4C9F07" modified="2011-01-11" version="1">MTRILERLAFWRKKQPRSELNNRNQPSSSRPLPPIQPTALSTPLENQTSFLVPDLDDIRPFVTSTSNPISMRDLLNAISASNHQPAIQQIPSTEACSADQLNVQLKYNQLSIRSKRKRDEADQQSNQSIESNQFNSFSSYNSHNQPGTICRATHIAITSSDTPPQFKKPKLVTISSSEDLEQLHNQLDCSQPPPPPPSSSSSSSRQHQIRRSSAPQPILGLPSHIWYQIFLYNQLDTIQALIPEHPSSSSSSSSNKASTLQSTNSIRSPKALSTHLKLALEAQQNRHALIGLCKTLSPIARKAAWSTLLLGTAKMVDQIAQRFEADSHLANQVQTCIITLLPPPRGRTGPQFDSPRRSTPGATENVSSKPSPLTPKQPGRSSHPSVRKIQNQPTQNRTPPTSRQSLPLARQALISSRSGQKCERFHSVLIPSLPPDKKVTTSRNTKLNSLSPLDDPQQPRRARTEADGVGEGPESEAYLDEIVLAGSLPSLFLSLARSMKLCCLIGEERLGTRGLKSLSVLSGLLGGLEELYVEGGGQFHDLVTLVDGLRSSTIRGKGAALRTVSVTGPHSTLSRIPSHSSTAESARSTLTCPLSQSSTSSNKPTQSHLSLEHLVLGNGIPLTPERLHWLVVSATGSPRGLRSLRVCLQADMDQPSLSSSVSSSSSSGSGRRMLSGRALLSRTFERVGGSLEWLALDEDWNAPLTGRLTRKVSFAGQPGEGILEEPVAFCSRLTVLSLPDGPLCSTGLLSVLPFTLQTIEIFDSGAPSNDDLSHSSSFSAHELWLAHSQAALFGLKTVQIVADSSTHSLFRKWKRAPSDALDHLIRAGVQFRWISAPSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3JYT5</accession>
    <name>E3JYT5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03166</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003321581.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JYT5"/>
    <dbReference type="GeneID" id="10537586"/>
    <dbReference type="KEGG" id="pgr:PGTG_03166"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03166"/>
    <dbReference type="HOGENOM" id="CLU_1846103_0_0_1"/>
    <dbReference type="InParanoid" id="E3JYT5"/>
    <dbReference type="OrthoDB" id="10374005at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171626" evidence="1">
        <location>
            <begin position="23"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="139" mass="15408" checksum="C23F1A068FCD6DAB" modified="2011-01-11" version="1">MKISAAHLVAIGLTVLASVVFGSTVGDPAAFSKVWDSKDLKELQSEPREVEGGNRLLFAEPVQEGKSTNTATKMRFGSYVISIVNSPNSNHRYSRYIKSPEDPPTHFHLLIQILTSCSLKFKWFSKLVHMYLPDLSPAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3JYV2</accession>
    <name>E3JYV2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FHA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_03183</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="OrthoDB" id="5348546at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000253">
        <property type="entry name" value="FHA_dom"/>
    </dbReference>
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        <property type="entry name" value="Msl-1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21656:SF2">
        <property type="entry name" value="MALE-SPECIFIC LETHAL 1 HOMOLOG"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    </feature>
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        </location>
    </feature>
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        <location>
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            <end position="473"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP77227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="597" mass="64726" checksum="7AAA6ADD6629B997" modified="2011-01-11" version="1">MLKSLDRLDHSATTNHINLKLLNPSSPATQLLSPTTINHSSPIISTNGNNTDSKIISPDNPSSSIHASKPSRSKRTPTPPIRIPIEPGHCLVFGRKPDISALANELTPKSTMLPITLPKSFTHASRTHCLCTLIAPPLGGLNIRIVVKGQNGLMVDGERFEEGSSAGVELERRDGEQIELGFYGGKKVECYVKIDDHSVQRVRARPNAHGQTNGTSNSSTTTSNIDTTTTTTTTTTTTTTTGNRPKHSTSTRGGKRKMPGGAASNSTRPDPTASKLKQQQNHSSKPEPSSPTTNIPLESPADTSKLPPWKYSSDPSAPPPPDRGMMSPPPAKKLCLSPIPSPPYDPQIHNLPPGDRVRSVIESMGLDLLGLIASAVVFSCRATVSTTELIRAVLDTQPTLAEAVLALLPSFSSSRSSPRPTHRASDEFELAIKKQRECLSAPLPSLYLPHDDPSTDTKPFESAASTTTATATTDFKPIRHHYGSEGGPADSPTLDFSPPSDLKTDRIVNACRPIILETLRSHWRANGSGMFGCVTNEGLKDASGEPLEALWYYQPQHDSDNERRLNLQPYVRHVRHTQTTSKQYFFKKVRGSGRRKS</sequence>
</entry>
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    <accession>E3JZE1</accession>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
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    <organism evidence="3 4">
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        <lineage>
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            <taxon>Puccinia</taxon>
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    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03372"/>
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    <dbReference type="InParanoid" id="E3JZE1"/>
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    <dbReference type="GO" id="GO:0005829">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="C:protein serine/threonine phosphatase complex"/>
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    <dbReference type="GO" id="GO:0019888">
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        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="PPP4R4"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN PHOSPHATASE 4 REGULATORY SUBUNIT 4 PPP4R4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21467:SF0">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 4 REGULATORY SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
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            <end position="82"/>
        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="818"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP77416.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="844" mass="93925" checksum="FD1AD0650657DF1C" modified="2012-04-18" version="2">MEAGRIKKRKRIPPTSDSDHQNNKNEQNNQNNQNSNKKKQIPATTTDENNKQLHTRSPSDSSSSSSNHPILNQHPQPSATDNINQDEELKQKLKNNFKFLILDGPGFYSNYQALKKMIESAQTCLEEEPDEAIYQTELDEKSAQLRAAELSFDDVLNTVASPVIEGLTHFIQAQVDRQLQTKLDQAIQAHSAHQEKKFEKNLKTLQHSHSQTEIKQLEKKFKEIENNHKTRLDHLQTKLASLEANLNKQAPQLSSTEGNSNETENNHKNHTDSRAPPGNALKLALSPQTSTAQSQPSIRHRSSSQSQSKSTHPDPNPTPVNPATRNSTGNTSQQRPNPLKIPVRVSVDAGGASPRPATAPADHSSLPSDSPVNTTNSAHHSTAPPPTAEALYEYISKRLMASKWLLDYSKVKLTDFYKQQIPQDLTNHLDQLGLTSQRISELLSFLSILPDIDLKFLSKKVATIATPTTPGNNPIVHLYPQQQPGPILNPNNALHLNPPTNIPVPSNFHLVENFSLTYLKDILGKIQFDANHLLIRLNRFEIRLGNAEKLGIDDHGAINALIKRMDFAETQTLPLVTSRATSENLHALETLVESKITAAQRETKDSVKALRDETTSAIKSLSRPSKPHTSPAGQSAHQGCLSMFKVENYILDRLFNEFERLSSRASSNTTKSKSKQRDRSEFEEGETSGEEDECKETVESILTDGRTNYSQTSGGNRVSIAGDPSARWKALGEMVYSQYQAQLQKEKDALVYNHQHQHHYHHHHHHRVRMLLGLDKNSEEDGAINCLKLGKQIDHQELNQAAELLVTEKRREEILAETSVSQSTAPSASSAAPDIPVNPASSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZG7</accession>
    <name>E3JZG7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="4">50S ribosomal protein L34e</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_03398</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL34 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321861.1">
        <property type="nucleotide sequence ID" value="XM_003321813.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZG7"/>
    <dbReference type="FunCoup" id="E3JZG7">
        <property type="interactions" value="410"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JZG7"/>
    <dbReference type="GeneID" id="10538320"/>
    <dbReference type="KEGG" id="pgr:PGTG_03398"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03398"/>
    <dbReference type="eggNOG" id="KOG1790">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_118652_1_1_1"/>
    <dbReference type="InParanoid" id="E3JZG7"/>
    <dbReference type="OMA" id="RCHKCVR"/>
    <dbReference type="OrthoDB" id="277449at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.370.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008195">
        <property type="entry name" value="Ribosomal_eL34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038562">
        <property type="entry name" value="Ribosomal_eL34_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10759">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01199">
        <property type="entry name" value="Ribosomal_L34e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01250">
        <property type="entry name" value="RIBOSOMALL34"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP77442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="116" mass="13004" checksum="69B9249904C33B4A" modified="2011-01-11" version="1">MVQRVTLRTRKSYNTTSNLRRIIKTPGGKLSYLRLHKQATSPKCGDCHTGLPGVPALRPVRYATVSKRIKTVQRPYGGSRCGNCVRDRIVRAFLVEEAKIVKKVLKSQAVPTKSKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZW1</accession>
    <name>E3JZW1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Proteasome assembly chaperone 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_03542</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322005.1">
        <property type="nucleotide sequence ID" value="XM_003321957.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZW1"/>
    <dbReference type="STRING" id="418459.E3JZW1"/>
    <dbReference type="GeneID" id="10538794"/>
    <dbReference type="KEGG" id="pgr:PGTG_03542"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03542"/>
    <dbReference type="eggNOG" id="ENOG502S9A3">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_105927_0_0_1"/>
    <dbReference type="InParanoid" id="E3JZW1"/>
    <dbReference type="OMA" id="GVMDMVQ"/>
    <dbReference type="OrthoDB" id="5593278at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018788">
        <property type="entry name" value="Proteasome_assmbl_chp_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053720">
        <property type="entry name" value="Psm_Assembly_Chaperone"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31051">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31051:SF1">
        <property type="entry name" value="PROTEASOME ASSEMBLY CHAPERONE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP77586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="176" mass="19158" checksum="8120A744F0EF621D" modified="2011-01-11" version="1">MMAAASSTIKAPIPVRQCARMIDSIHTEVMTQGYSDRTLVLVTQTGKIGSLTQVTIPLASFEQGFELSSGSENGLLPALPVPFTSLQLIPLLSSTPPELKALYDVYLNQIAMLVFTGFTPDASSHQRSCSPDRITKPVIIGLALARLPSDTDQDVTDLERARFSAIMNMVMECKLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>E3K0C0</accession>
    <name>E3K0C0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_03701</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
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            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
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</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3K0N3</accession>
    <name>E3K0N3_PUCGT</name>
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        <recommendedName>
            <fullName evidence="5">ATP-dependent DNA ligase family profile domain-containing protein</fullName>
        </recommendedName>
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        <name evidence="6" type="ORF">PGTG_03814</name>
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="project" value="UniProtKB-KW"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="InterPro"/>
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:Okazaki fragment processing involved in mitotic DNA replication"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA ligase IV"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
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        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="ATP-dep_DNA_ligase"/>
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        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
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        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
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    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
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    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674:SF12">
        <property type="entry name" value="ATP DEPENDENT DNA LIGASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00333">
        <property type="entry name" value="DNA_LIGASE_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="5">
        <location>
            <begin position="439"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="753"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1048"/>
            <end position="1141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="783"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="807"/>
            <end position="827"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="845"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="942"/>
            <end position="954"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="972"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1003"/>
            <end position="1015"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1074"/>
            <end position="1084"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="1093"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="1120"/>
            <end position="1134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP77858.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1262" mass="141033" checksum="944FF984A35C99CF" modified="2012-04-18" version="2">MAPKIYVKRPPTAVPHEITFATFCMYLDKIASRKPRKAGTRNPATYDSADKAAKDFETWINHLSWPVPKHTGKILFRLLFPDHDVRRKYDIREKKLAGFFRMIFHVSTGPDQDGIQTGKAGCLGLELQKILEDVQVGQTGSLKLSVVDRLLDELAARSKWSNLGYHIPQDHSHRSATAILYDLYAPLSPRESAYMTQASDSEEHSIILKDLRPLLYPIPSLSVYISLMDYDSTAFKEISPYCMMKAWHWAMPRIYRAKADFDVAADLCERIPRGMLHPSEIPKTVKASGPCLAAPLSKLTGQVWAETKMDGERMQIHIDTSKPQNEQIKILSKSHRDSTSERAPTHSLIRAALGFNLSSAPEFLTSTPRPATLKKIESGIFEAEMVAWNLSQGHVDEFWRIREMKLKPWQTYNERQEIDDLSSAFTESQIGQMDTQQLSDLESRHLALCFFDVLYLNGESLLERSYNERRHALEKCIHVIPHYSMLVERKSFDLSRESGKEALRKHYAAVIAERHEGLVLKTAESVYNDSRTEHKWLKVKKDFIQGFGDTADYAIIGASWDQNRGRELGVPTSTYVSWYVGLCDNTLDIKRSPGIRPHFQAVFTSLSYGLSRQQLEQANRTAKEIGGEPPNSKALKKWSFTYELARELSQPAIIFKKPLVFELMGSGFTKRSRNHEYELRWPRIMKFHDPKERDWTTAIDLETHDEMAKRATDPKLLGIADIISGGSQEEAANGFRAELEHWDNKLKTSDLKWLSRGSKSRSDSSSTQLTAPKPVKEIPSIIPENSIQTGSSKATNEMDSSKKKPSKTVQRNESSSHVATKAATSQKGKAPMETGNPDNPEIILDEERPARSRLPPSKEIKPTRKQPSRACSQATAPHVDGIDPTKPLLKRKASGSMTTPAEIPLRGSRLATTAKLDKAAPGAPPTALESKDRTGPNVKRQKTSEHNPHSEALPDKANILGPTAEQDIPTLPKSTSSKDTNPSVKTSEKSAIPMDATEIPTAKRTSTLARVQSTTSSAIGLTPKHRDFFAYDLLDIEKEGILSCPDVADNSKPVLSPAARNSTLPDKPLAKDSSIPTQTNQSASHVPASDRIPAPSVSTSGPGPSTSLSRAPGGIGTGANIEQSGPTETATDGNHSAPPWHQRSDIRWCLVQDEQTYGIQGTRYGSVNQLLRSLEIDDKKFDQNRQPESFSYIFIENPSPEKIQRIQKQILFRPLSDPTVDIVGYDAKVLTLQNAPLNQAWKSFELFRFNSYTHQFDPVQTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K0Q2</accession>
    <name>E3K0Q2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_03833</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322296.1">
        <property type="nucleotide sequence ID" value="XM_003322248.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K0Q2"/>
    <dbReference type="GeneID" id="10540863"/>
    <dbReference type="KEGG" id="pgr:PGTG_03833"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03833"/>
    <dbReference type="HOGENOM" id="CLU_628718_0_0_1"/>
    <dbReference type="InParanoid" id="E3K0Q2"/>
    <dbReference type="OrthoDB" id="2495363at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173131" evidence="2">
        <location>
            <begin position="21"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="336"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="338"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP77877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="356" mass="41721" checksum="2F2DE0E98EB7ED81" modified="2011-01-11" version="1">MKTLLTTPCLMLLAMGGTSSSPPTDSTWAIQKLSNFESIISYDELVNQILKEPLCFHEPTLHSQFSGPNFRPQANHQELNDKMVYSPQQLTASDQGLPRPPKRKWHVILTEGAENQSPSKQTEIVEECPKSPAEETRSMLLEGADLDPDEIMRKNEELMRNTSDFVPHAQLDMQEEKSKEPSIPTKLFHVYNWEYVKQSPQDQTTERNNADRLEELFSLLNSCKTIPPQGPLFWIPRENVHKFLKREEVNKILCFMRDLWKDMEQEELLLEPSKYTWIKRSSDMFRLEEKQDDLRNEKAWANGYSLWYFVAEDIVKYWTESNRNKFKLRWGKREYPSEQPSGNIANLEESSWEATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3K1D8</accession>
    <name>E3K1D8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04069</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP78113.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322532.2">
        <property type="nucleotide sequence ID" value="XM_003322484.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K1D8"/>
    <dbReference type="GeneID" id="10541583"/>
    <dbReference type="KEGG" id="pgr:PGTG_04069"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04069"/>
    <dbReference type="eggNOG" id="ENOG502T1DR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_054434_2_0_1"/>
    <dbReference type="InParanoid" id="E3K1D8"/>
    <dbReference type="OrthoDB" id="2802833at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78113.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="162" mass="18546" checksum="1A3B7EE5CFA3E898" modified="2012-04-18" version="2">MNNLSALIQTIPTLEGNSVTFPYWRNRLQNVLQIQGVLDIVNKMLPRPNNTDSKEGKLAVQSPEQRGYNPEEYGSDWDTLSNMAISTIQLTLLVDLSIRYEGVKPAHKLFSTICDAYEKNTWARRLQLEDAFWTARQDPSQPIEKLEMVNSHPDIRLLFAAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3K1E3</accession>
    <name>E3K1E3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">rRNA-processing protein las1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_04074</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP78118.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322537.2">
        <property type="nucleotide sequence ID" value="XM_003322489.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K1E3"/>
    <dbReference type="FunCoup" id="E3K1E3">
        <property type="interactions" value="5"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K1E3"/>
    <dbReference type="GeneID" id="10541588"/>
    <dbReference type="KEGG" id="pgr:PGTG_04074"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04074"/>
    <dbReference type="eggNOG" id="KOG2425">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031483_0_0_1"/>
    <dbReference type="InParanoid" id="E3K1E3"/>
    <dbReference type="OrthoDB" id="10263222at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090730">
        <property type="term" value="C:Las1 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000460">
        <property type="term" value="P:maturation of 5.8S rRNA"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0000470">
        <property type="term" value="P:maturation of LSU-rRNA"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007174">
        <property type="entry name" value="Las1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15002">
        <property type="entry name" value="RIBOSOMAL BIOGENESIS PROTEIN LAS1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15002:SF0">
        <property type="entry name" value="RIBOSOMAL BIOGENESIS PROTEIN LAS1L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04031">
        <property type="entry name" value="Las1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP78118.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="486" mass="54046" checksum="3D77172DC2288D5B" modified="2012-04-18" version="2">MQYGAQDVCFGSGGSEVPCRVFPFITMSPALARKVPWSSPAEFIGLFDLLFSSADPLASQPIALNHLYAWSIRSPSSLPPAIEATHALLSLILPPTPNVQQQRLGLAMALSRLVNSLVDPLQKGMYARSISQIAAELGLPLALVQLRHRATHEDLPSLTILLQSANLALDWLYTHYWLPRLNDIEGKGHLKLETIGLLEGLLISFKRARKRAILDTTQVDFLQFLPQIDHWIGAAARELSGDGSQSRLAEDDGQADHRPLVALCSVLVERQVLVPQAVKKRCQSSKDPLPEPLKDLYEPLIDHLAQSHPQVFIRSLVMSLLDTLSALPDGTSTSKQADATYYNTCASWLVYLLLRADGGPIGPEALRILLIKPNQYTLSVVERLKEAKQSDWYDEKTLSPLIDLLQKTRDDQRNDMDEGCMSEEPIEDRIAAMKQRWECLVAKDEEPVVQEGSSWNLVPEANWVPCPIGCLPDRTVPDLYKSLLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>E3K1Q3</accession>
    <name>E3K1Q3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein bS18m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_04184</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial ribosomal protein bS18 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP78228.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322647.2">
        <property type="nucleotide sequence ID" value="XM_003322599.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K1Q3"/>
    <dbReference type="STRING" id="418459.E3K1Q3"/>
    <dbReference type="GeneID" id="10542020"/>
    <dbReference type="KEGG" id="pgr:PGTG_04184"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04184"/>
    <dbReference type="eggNOG" id="KOG3162">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_108147_0_0_1"/>
    <dbReference type="InParanoid" id="E3K1Q3"/>
    <dbReference type="OrthoDB" id="21463at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.640.10">
        <property type="entry name" value="Ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001648">
        <property type="entry name" value="Ribosomal_bS18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036870">
        <property type="entry name" value="Ribosomal_bS18_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13479">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13479:SF40">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN BS18M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01084">
        <property type="entry name" value="Ribosomal_S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00974">
        <property type="entry name" value="RIBOSOMALS18"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46911">
        <property type="entry name" value="Ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="203"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP78228.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="223" mass="25428" checksum="E4E56F0875A0FC74" modified="2012-04-18" version="2">MNFNSCQKLWGTSTCISRSIGSAKNATTPLNAYSRTHFSSQATLHSENEQNKMTLSKLVEKEVKAKPLNPLPTFGSQWGKYFSDGSRRLSFNPNQVLKPSDLQLENLIHPADRESRKPRPVVRRPPTKDEVRLTDPFLYYNINILKEPYNPDLLANFITPLGRIKKRALTGLSKGNQKKVSKAIRRARCMGFLPYFGKPLERYSEDHQQRRDGPGVDLPPFAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3K241</accession>
    <name>E3K241_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Carnitine O-acetyltransferase, mitochondrial</fullName>
            <ecNumber evidence="16">2.3.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PGTG_04366</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Carnitine acetylase is specific for short chain fatty acids. Carnitine acetylase seems to affect the flux through the pyruvate dehydrogenase complex. It may be involved as well in the transport of acetyl-CoA into mitochondria.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(R)-carnitine + acetyl-CoA = O-acetyl-(R)-carnitine + CoA</text>
            <dbReference type="Rhea" id="RHEA:21136"/>
            <dbReference type="ChEBI" id="CHEBI:16347"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57589"/>
            <dbReference type="EC" id="2.3.1.7"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Peroxisome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the carnitine/choline acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.7" evidence="16"/>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322829.1">
        <property type="nucleotide sequence ID" value="XM_003322781.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K241"/>
    <dbReference type="STRING" id="418459.E3K241"/>
    <dbReference type="GeneID" id="10536008"/>
    <dbReference type="KEGG" id="pgr:PGTG_04366"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04366"/>
    <dbReference type="eggNOG" id="KOG3717">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013513_5_1_1"/>
    <dbReference type="InParanoid" id="E3K241"/>
    <dbReference type="OMA" id="KMDGTPT"/>
    <dbReference type="OrthoDB" id="240216at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004092">
        <property type="term" value="F:carnitine O-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009437">
        <property type="term" value="P:carnitine metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.559.70:FF:000007">
        <property type="entry name" value="Carnitine O-acetyltransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.10">
        <property type="entry name" value="Chloramphenicol acetyltransferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.559.70">
        <property type="entry name" value="Choline/Carnitine o-acyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000542">
        <property type="entry name" value="Carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023213">
        <property type="entry name" value="CAT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039551">
        <property type="entry name" value="Cho/carn_acyl_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042231">
        <property type="entry name" value="Cho/carn_acyl_trans_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589:SF103">
        <property type="entry name" value="CARNITINE O-ACETYL-TRANSFERASE, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22589">
        <property type="entry name" value="CARNITINE O-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00755">
        <property type="entry name" value="Carn_acyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52777">
        <property type="entry name" value="CoA-dependent acyltransferases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00439">
        <property type="entry name" value="ACYLTRANSF_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="12" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Choline/carnitine acyltransferase" evidence="19">
        <location>
            <begin position="50"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="18">
        <location>
            <position position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00052702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00053195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00066910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00073438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR600542-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="EFP78410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="643" mass="72505" checksum="D1E573A6810F4DDD" modified="2011-01-11" version="1">MFSLRRTALHSISTGRLLTSNSFQANRSTMASTRSKSTITYQHQDSLPHLPVPELESTAQKYFRSILPLVSPQDPHSPTASDGTPTPAYKHTKACVEDFLKSPLVKELQNRLKKRAHEEGRESWLSEWWNELAYMAYRDPLIPFSSYYIAHKVDHHRRTGPKRAAGLVRAMMEFRHLTETEQLEPEHSRTGPLCMNSYRWLFNSCRYPVKPSDTARKFDPQVNTHLVVIRKGQFFELPAVKPDGTLLSEAELESQFNKVIQLAGPTETPFPVGALTTEHRDIWADMREELCKTDPHNSKSLERIESGIVCVALDDTSPITRDELGFTIWSGGGKNRFFDKQQLIVCENGQSGFNAEHSCMDGTPVSRMNDWILDALANKKIDLGSSFDSNLPAPTPIEFVLSDTTKQNILRSVTKFHNLMSLQTLDVLEYPGYGKRVIKNQFKTSPDAIAQLTFQLGHYKLFGRVPVTYESCQTRKFKLGRTEVIRSCSIEALEWCKAMEDVNADWPTRLEKFKLAAKAHLAYAKEASDGQGVDRHLLGLRLSLKPGEEMPALFQDPIYKESTNWILSTSTLPSEYFNGLGYGAVVPEGFGLAYAVNEESMRFTVTTTTGNGARLKYCLHEAADDIHKMMRSQDGKGVPSAKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>E3K250</accession>
    <name>E3K250_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">1-phosphatidylinositol-3-phosphate 5-kinase</fullName>
            <ecNumber evidence="1">2.7.1.150</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_04375</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EC" id="2.7.1.150" evidence="1"/>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78419.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322838.2">
        <property type="nucleotide sequence ID" value="XM_003322790.2"/>
    </dbReference>
    <dbReference type="FunCoup" id="E3K250">
        <property type="interactions" value="578"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K250"/>
    <dbReference type="GeneID" id="10536017"/>
    <dbReference type="KEGG" id="pgr:PGTG_04375"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04375"/>
    <dbReference type="eggNOG" id="KOG0230">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000480_3_0_1"/>
    <dbReference type="InParanoid" id="E3K250"/>
    <dbReference type="OrthoDB" id="158357at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010008">
        <property type="term" value="C:endosome membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000285">
        <property type="term" value="F:1-phosphatidylinositol-3-phosphate 5-kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007033">
        <property type="term" value="P:vacuole organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17300">
        <property type="entry name" value="PIPKc_PIKfyve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000007">
        <property type="entry name" value="1-phosphatidylinositol 3-phosphate 5-kinase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000283">
        <property type="entry name" value="1-phosphatidylinositol-3-phosphate 5-kinase (Fab1)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.810.10:FF:000013">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.810.10">
        <property type="entry name" value="2-Layer Sandwich"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.800.10">
        <property type="entry name" value="Phosphatidylinositol Phosphate Kinase II Beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044769">
        <property type="entry name" value="PIKfyve_PIPKc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027483">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002498">
        <property type="entry name" value="PInositol-4-P-4/5-kinase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027484">
        <property type="entry name" value="PInositol-4-P-5-kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000306">
        <property type="entry name" value="Znf_FYVE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017455">
        <property type="entry name" value="Znf_FYVE-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45748">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 3-PHOSPHATE 5-KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45748:SF7">
        <property type="entry name" value="1-PHOSPHATIDYLINOSITOL 3-PHOSPHATE 5-KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01363">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01504">
        <property type="entry name" value="PIP5K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00064">
        <property type="entry name" value="FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00330">
        <property type="entry name" value="PIPKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51455">
        <property type="entry name" value="PIPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50178">
        <property type="entry name" value="ZF_FYVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 10" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 9" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="FYVE-type" evidence="12">
        <location>
            <begin position="345"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="domain" description="PIPK" evidence="13">
        <location>
            <begin position="2232"/>
            <end position="2552"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="48"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="105"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="175"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="290"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="438"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="557"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="591"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1066"/>
            <end position="1109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1226"/>
            <end position="1247"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1591"/>
            <end position="1619"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1635"/>
            <end position="1663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1701"/>
            <end position="1961"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2008"/>
            <end position="2031"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2043"/>
            <end position="2114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="2569"/>
            <end position="2604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="51"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="118"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="290"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="438"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="511"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="563"/>
            <end position="578"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="616"/>
            <end position="625"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1072"/>
            <end position="1090"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1231"/>
            <end position="1246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1644"/>
            <end position="1654"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="1714"/>
            <end position="1728"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1882"/>
            <end position="1901"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1908"/>
            <end position="1926"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1935"/>
            <end position="1944"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="2008"/>
            <end position="2020"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="2579"/>
            <end position="2597"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EFP78419.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="2604" mass="288281" checksum="5EB8E07CF7F8A249" modified="2012-04-18" version="2">MHSSLFSSERTIRPSSGGRSRDASPAPSSFHADLRQFSYVSFPETTGFLPSRSQKAQPTLGTDHSLGFLPDPQPGKSLLGRFKSAIGASAWISPATTSEHKTSTIALPTHPSLPPMTMSYSQADLDSSNSFDSRKSRAKKISLNQSSSRSFGGSLFEPSDSSRAQNMESYQYSHPQGLVGSQHRSSFGHSESPPRPGSSMSFRALNRSVDGLLEAQRPASTALDSSRAPFHFNSLPEPENAFPLVSSDTLLRSLVLDNSGGNHSADFSAVPPFSHIPGFPLARETVGDDSASVFSSSGGPSSTPKKIRPNNQDPSHNLSRETSSRLHAIGNPQGKGLSKEYWLPDKDVRECYDCAVAFTSWRRKHHCRICGAIFCSQCASNLVPGNRFASSGYIRVCNICLAKLEPQSQEQTNFAIREDTDDETQSFSEAASGFNLLASRSQGNRAHTSTPGKMREAARQVGDQLASSVTHRTGRSLYRNPPDHHSARRKSLLRQLAPPDKTRSKFHSRSDSPPVSRSSPITANLFSPLTIKRSSLQKPEAVSSAPFRKGLTDEEQAGLPGISTANHTQSVYVSSPNTSKLSAEIKEAPLDRHFSSSSHETNHDSSQNGERHEQRLPSTSRNASGSYVEGQPKPCLASELPFSKAASASFVSETEQSLRLEESFSYFPAFSCEHVRFMIHQFLTRENIPKTPVWETELTNLLLQLVSEPPRPKYDAEDAGDIRRLVKIKRIPGGQVADCEYIHGVVFTKNIAHRKMKSECSVPKVLTIGMPIEFQRVDGYCKLDVLVSQERQYLKGLVHRLISLEPDLVLVDGNVSGVAIDYFVEAGVTVLRHVKSTVLQAVARSTKTPLISSLDKLLNLQVGQCDLFRVQTVHHRMIPNHHKKTFIRLEGCEPSLGGTLILRGGDLALLTKVKSLMCSMVGIIYSLRLEDSFLNDEGAIYSNDSTHETLLHQIRAIDLRTVLSRFRHAKKHICNFEPLKLPGKPGGAIPHSDLITLSDQVDDLIKPYECLLPSGSPRVNLTPPFALLKLRQLIQQLIDLIELDTQSIQPLSAKSCLHDEKFDEKLHPERSPSMSLTSSHTKSNDSNSILSDEATTRVRQPTITPDARDEYISHLNRQIEEERMAVHFYLSNHSSETFKAADHQQLIVQESVTCSSLGGYTCQGPHLRAFAFYSAGDQSVGQIIQMLINCRSEICHAKGCNQPKSIHQTNWIHGHYKATIQMHLGHSNRQSASSDSEPSDPSNFPEEPLDPDLIMMQGYCPKCEGYTRRTPMSDDAWRLSLGKYLQLCLYSPGLVSNLMPGTPRRGRLSCSHDAHLEHLRMFFYQGFRVDFRLQKINVYEAIPPSLVLFTPSQALIKVREEEYEIILRRSEAFFNSVRSRIVGFNYKVVSLANQDASEEAMRDLLKKVDVDSNAVKAQLNEVYEECVGTNGVKMNIVRKTLIDKAVEWDGLFATFESRFVATDGKDARRLASVPLKKIFLDQSMPGSPERLFPRISQAPSLNSQKYNYNSQSEVESDHAPKGQSFGTFKKTFPSPTLKSMASALAFALPSSMPGIGIPEIRSDGEKLKNHVPHPLGPPLCVLQPSQEVAPKDGLMLDSNPGVDASNDEPHSSGELSIPQKSLVELSIPSVVIEPASTPTSTASNTQKAGTNSNVESHDPKGKLKDATEISAEIQQSPASTSNTQAGHASNHRISLLTASRHDSYDLEPQPNEGTHPDLVSDKEEDSKSDSTVVNVRSGVALSSHSKGSDSQRKKCAASDTDTTTSELDLPNDSRRMTLRSRRPKDLVLNSRGGVANLVQKFESPKPSPRKATRSNSPVIDESVTKSSNAEEGSAKKKPLKTNALPIRPSFRRGKSEIVRSTMNVLRGKKPSAGLDKAGSHIDCNQSFDSSTPRLSGKQSVQEHIVRVPSRTQRPSSRITSPRTPSSHVHSHRSPMQRSGTNDGASSAEKPRVPSSPRGLGLKKEFAVPRRARHESGLPVSNFYLHRRMNMNTKVTTIARHFDRMSRDAERERIKQANEHRSRPRPIAHAKSSVQAFSSLTAAVNEDSDSDIDECEGHPAGHDADDEGDVIPEAYGPDNLAQGGFKAEPGLSTDLPLVPEVTPAGPHPSPDLDHIDQSYLDEASTSNRPLSPSRVTSCSPPFLTDTSRLPPLAVAHFSDNEMSSSDTGRHSIIKTLSSLWNYQGAEFLPLEYPQLPTEHQSAENPVIIREDEPSSIIAYTLASKLYQATLKETEPRVVERSEIFMPEEFKPRSNDIDSTWGMIDFMSDDLDVDDVLKIPMNRAKPMQFRFDVTPCTITCKVFFMHQFEALRKTLICKDIVESLARCHKWDASGGKSGQKFLKTKDERYLIKGISKAELEALTKFAPAYFEYLSSAIKEKRPITLAKMFGIFQVSFVNKITNRKGRLQVQVIENLWVTKPHLQIYDLKGLTRNRTVNVTGKPHEVLLDGNFCEMVRTTPILVRENSLLRLQASLHNDTLFLANHNVMDYSLAVGFDEEKQEMYIGIIDYLQTYSWDKRLETLVKELGGTSKEAPTVITPSLYKARFRRAMGKYFSVAPDPWMHKWKPYLSPSEEDSIGASVDKEGSRLAETGAKAEDSYVHLSGNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2I6</accession>
    <name>E3K2I6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Clathrin light chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_04511</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 6">Cytoplasmic vesicle membrane</location>
            <topology evidence="1 6">Peripheral membrane protein</topology>
            <orientation evidence="1 6">Cytoplasmic side</orientation>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Membrane</location>
            <location evidence="6">Coated pit</location>
            <topology evidence="6">Peripheral membrane protein</topology>
            <orientation evidence="6">Cytoplasmic side</orientation>
        </subcellularLocation>
        <text evidence="6">Cytoplasmic face of coated pits and vesicles.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the clathrin light chain family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322974.1">
        <property type="nucleotide sequence ID" value="XM_003322926.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2I6"/>
    <dbReference type="FunCoup" id="E3K2I6">
        <property type="interactions" value="230"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K2I6"/>
    <dbReference type="GeneID" id="10536319"/>
    <dbReference type="KEGG" id="pgr:PGTG_04511"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04511"/>
    <dbReference type="eggNOG" id="KOG4031">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_069856_1_0_1"/>
    <dbReference type="InParanoid" id="E3K2I6"/>
    <dbReference type="OMA" id="FYENYNT"/>
    <dbReference type="OrthoDB" id="5512at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030132">
        <property type="term" value="C:clathrin coat of coated pit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030130">
        <property type="term" value="C:clathrin coat of trans-Golgi network vesicle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030125">
        <property type="term" value="C:clathrin vesicle coat"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032050">
        <property type="term" value="F:clathrin heavy chain binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000996">
        <property type="entry name" value="Clathrin_L-chain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10639">
        <property type="entry name" value="CLATHRIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10639:SF7">
        <property type="entry name" value="CLATHRIN LIGHT CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01086">
        <property type="entry name" value="Clathrin_lg_ch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0168">Coated pit</keyword>
    <keyword evidence="5 6" id="KW-0968">Cytoplasmic vesicle</keyword>
    <keyword evidence="3 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="49"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="95"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP78555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="260" mass="28901" checksum="D5D6273F38C17450" modified="2011-01-11" version="1">MSDFPELEDFSNVAASNDPQADFLARERALLGDEFGAPPENYQETIQVKQDDDDQFGLGDFSSAPIDHHPQPAAPPPVSTTGAPDELASFQDQYPDISHDIPDQTNGFDAPAPSLYANQSSAYATPAVYAQTQTEEESPFIKEWRTNQAEEIRVRDERSAAKREETIVAAEKAIDEFYHGYNTEKQKNIAKNKEEEAKFIQSRSDKLAQGTTWERIAELIELQDSRSKTCGKSAKDLARFKEILLNLKRDGQNAPGAAGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3K303</accession>
    <name>E3K303_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_04816</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the glycosyl hydrolase 32 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78860.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323279.2">
        <property type="nucleotide sequence ID" value="XM_003323231.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K303"/>
    <dbReference type="STRING" id="418459.E3K303"/>
    <dbReference type="GeneID" id="10536964"/>
    <dbReference type="KEGG" id="pgr:PGTG_04816"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04816"/>
    <dbReference type="eggNOG" id="KOG0228">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013784_3_0_1"/>
    <dbReference type="InParanoid" id="E3K303"/>
    <dbReference type="OrthoDB" id="202537at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004575">
        <property type="term" value="F:sucrose alpha-glucosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005987">
        <property type="term" value="P:sucrose catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18621">
        <property type="entry name" value="GH32_XdINV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.115.10.20:FF:000016">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.560">
        <property type="entry name" value="Exo-inulinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.115.10.20">
        <property type="entry name" value="Glycosyl hydrolase domain, family 43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013320">
        <property type="entry name" value="ConA-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001362">
        <property type="entry name" value="Glyco_hydro_32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013189">
        <property type="entry name" value="Glyco_hydro_32_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013148">
        <property type="entry name" value="Glyco_hydro_32_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023296">
        <property type="entry name" value="Glyco_hydro_beta-prop_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42800">
        <property type="entry name" value="EXOINULINASE INUD (AFU_ORTHOLOGUE AFUA_5G00480)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42800:SF3">
        <property type="entry name" value="GLYCOSYL HYDROLASE FAMILY 32 N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08244">
        <property type="entry name" value="Glyco_hydro_32C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00251">
        <property type="entry name" value="Glyco_hydro_32N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00640">
        <property type="entry name" value="Glyco_32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75005">
        <property type="entry name" value="Arabinanase/levansucrase/invertase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49899">
        <property type="entry name" value="Concanavalin A-like lectins/glucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyl hydrolase family 32 N-terminal" evidence="5">
        <location>
            <begin position="111"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl hydrolase family 32 C-terminal" evidence="6">
        <location>
            <begin position="564"/>
            <end position="703"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP78860.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="777" mass="87413" checksum="1253E04531F18D22" modified="2012-04-18" version="2">MTRKHDLPTNNRRTTTWVALHLLYGALLLGSFAGASSLLQLAKREEHPQDTFAADKAGPNLSSHNNFPSFLNSTSDSCRIDRSQPPVLDQLIGCKNSSLFHLWRTKARVTGPRGWMNDPMAIFETKSGAYHVGYQCNPDHLVWANISQCSAFTTDFVHFKDHHSWKDPRTIVPTQLYDIRGVFDGTVIKDGWNGHPSIIYTSVFTGPIGARSNPPELEGVETQSVAYTEDDGHTWTKLNFGANGNPVIYKWPENHLSGFRDPFVFKSSEFSRFYANESIVHQLPGNPNALKPSGHNYLLLSGGIRTEVDPIHGGPRLFLYRQTEENNIRDWTYLGPLVSSIAERNQTSEWTGAKGINYECGAVTKLDEHGLVKQEDDSADSKTQLNIIATGSEGGRTGPGYWPIWHAVSWDFDADDGNVRSKIDFSGVIDWGKAYAFHLFESKNRQLLVGWTYEDDEKNILTPQRGAQGAFTLFRELFVQVLRNIHPAALKSPEHAPSWTTRTEADGSHSLMTVGQRILPEISSAFRKHSKVSALPPQTLSPGYNPGAHQHSLSTNAVRLEKQPKDRYYAISARLEFYADRRELDGEDVGRAKMSRGGFRILSSQNEWTDVYYDPSTEYLVVDRSRSSAIPSFGNSSERAKLRLWPIVNPVTKRVEIEPLNLTIIVDNSVVEVHANERAIITTRVYPWYEDSIGIDYLVEGRPTVVDELHLARHSALSENQNRLGSHAFQPHSVHFTNVEIWDGLVNAWPDRPRDTRLPGVYSHNITSSLYGLWPDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3K542</accession>
    <name>E3K542_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ACB domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_05678</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79357.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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        <property type="entry name" value="COA BINDING PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G12300)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Acyl-CoA binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="87"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
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        </location>
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            <begin position="222"/>
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        </location>
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            <begin position="366"/>
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            <begin position="426"/>
            <end position="465"/>
        </location>
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            <begin position="10"/>
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        <location>
            <begin position="40"/>
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            <begin position="73"/>
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    </feature>
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            <begin position="272"/>
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        </location>
    </feature>
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        </location>
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        <location>
            <begin position="453"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51228"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP79357.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="508" mass="56089" checksum="29CCEC4035447B88" modified="2012-04-18" version="2">MPTRTGGNPSGSRPLSSSSRLENFPKNGLGQPYTHKQQHSSSIGSSSTSLLAKSSVLPPSNPETSNESPPSNHSHDNSQSDHEEAFIEPLFQTALHIVQSSLPRSGQIQPTYNDKLLLYSLFKQAVHGDVKSTISKRPGVFDMLGRAKWDAWKAREGLSCSEAKRLYVESVLKILRSHRDRPEARLLIESLSGYEAYLATVDHMSDSDEDLYSERKIEIPASMRGSIHTLSGQEQAQEGAEEEERSEENESAGSKSDEKSADQLEEEEEDIQTSGLGPYKSSSQLPDPINSSTETHILPPHSPRAIEPSSAASSESLSQSPSPKDDSSPAQASQESIQSTHIEPSEPRFTYAQVQRLVDQTPQTLPFHSLRSPLNHPTHSHPDLSRLTLPQSSHDPYSSSLALLQSNLEKLNQTIDLLHTLISKIHPPQHHSSTSSPPPPPSALNKNILVATPSSSPSSNPHHKSYSLLSLSRLRTVLNHPWVKRLFLDLSLFFLLLKARKFFNKRPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3K6Y1</accession>
    <name>E3K6Y1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05180</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003324326.2"/>
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    <dbReference type="InParanoid" id="E3K6Y1"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173581" description="Secreted protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="136" mass="14785" checksum="54DE8D89D6C20D78" modified="2011-01-11" version="1">MNDFFSCLVIFGLVALSQAKSVSTQPQIIRPVNYSLVKSSQDNTHSWLINQNLGPKYNPMSCSTCEMFVIAQDCKPVHNDANVAPASVSCDKAYLPAKDPKTQPTSCQTRSSGDFNCSDRKDGSKIPVCHACLPIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K783</accession>
    <name>E3K783_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_05218</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EFP79993.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324412.2">
        <property type="nucleotide sequence ID" value="XM_003324364.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K783"/>
    <dbReference type="GeneID" id="10533847"/>
    <dbReference type="KEGG" id="pgr:PGTG_05218"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05218"/>
    <dbReference type="HOGENOM" id="CLU_1778374_0_0_1"/>
    <dbReference type="InParanoid" id="E3K783"/>
    <dbReference type="OrthoDB" id="2504822at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP79993.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="153" mass="17519" checksum="8E24F9460023A5A7" modified="2012-04-18" version="2">MVFINLKLLSIKLRDDGDSQPVKLDPKPPESFEALSKEIKSANRLIKQFENDMKERPNRLLSGSMRELIEQVMGQLSLLKGTNDEELSKIAICAKALADITAAKQPTTYQITELESTSLKYIMVVWDNYIQPSQNERVNSFFENLRILWTSKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3K8U0</accession>
    <name>E3K8U0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_06602</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325065.1">
        <property type="nucleotide sequence ID" value="XM_003325017.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K8U0"/>
    <dbReference type="GeneID" id="10539662"/>
    <dbReference type="KEGG" id="pgr:PGTG_06602"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06602"/>
    <dbReference type="HOGENOM" id="CLU_1403068_0_0_1"/>
    <dbReference type="InParanoid" id="E3K8U0"/>
    <dbReference type="OrthoDB" id="10438666at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP80646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="194" mass="21788" checksum="D99B61A0951E50D2" modified="2011-01-11" version="1">MGSDHIRQAWSLRVCLLQICLVAFIGKSTCFIEVQKILGSTDDQWLGYTRARLSATQTLGTSFGLLPPKALGQRITGDRSSLFKSSGYWGQNEPESEKDLYKKFSGLIHSYFQDTIGFSIPEVEDIVHSIQNDINHLIEKAFHNEGTNKQKSKTMAIELYGVQSQQEGKEKFVPGDLSLEGSPFTQELMDFLEC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3K8W4</accession>
    <name>E3K8W4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">[Histone H3]-trimethyl-L-lysine(9) demethylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_06509</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80553.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324972.2">
        <property type="nucleotide sequence ID" value="XM_003324924.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K8W4"/>
    <dbReference type="STRING" id="418459.E3K8W4"/>
    <dbReference type="GeneID" id="10539564"/>
    <dbReference type="KEGG" id="pgr:PGTG_06509"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06509"/>
    <dbReference type="eggNOG" id="KOG0958">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008312_0_0_1"/>
    <dbReference type="InParanoid" id="E3K8W4"/>
    <dbReference type="OrthoDB" id="9547406at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051864">
        <property type="term" value="F:histone H3K36 demethylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032454">
        <property type="term" value="F:histone H3K9 demethylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.650:FF:000149">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.650">
        <property type="entry name" value="Cupin"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003347">
        <property type="entry name" value="JmjC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF7">
        <property type="entry name" value="[HISTONE H3]-TRIMETHYL-L-LYSINE(9) DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02373">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02375">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00558">
        <property type="entry name" value="JmjC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00545">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51197">
        <property type="entry name" value="Clavaminate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51184">
        <property type="entry name" value="JMJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51183">
        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="JmjN" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="JmjC" evidence="3">
        <location>
            <begin position="499"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="95"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="244"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="376"/>
            <end position="442"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="686"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="123"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="155"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="196"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="408"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="686"/>
            <end position="704"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP80553.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="902" mass="100793" checksum="C8950B98E466AA4D" modified="2012-04-18" version="2">MEQFRNFYSYINQIDRWGMRSGIVKIIPPKEWSEQLPHLGQESLRTDGKGQMLRNAKIKSPISQVIQGSRGLFRVMNVAKRKTYNALEWYDLANSDEHRPPDFSNKTLDTSSANFSQDHPDMTSTRSTRSSRRSTSSIAPISHRIANPRRRKVSKGTSTTETSTVDQTSPEKPAVEGPIECSTEKRNNEEPVNCSPEKRDDEEKNVNCPSLKNPHVMQSVTCSPEKQDLGHHFDCSPSKLLPVPEDQPLPCPGAKDPESPKPAPSLTVPLPQCSTDAIALDSRELAPSLPVPLPQCSTDIMASDSREPAPSAPVFLPQCSTSELSNAAMNHEDCPKKDAGIMASCSAEPDRQQDDSNTIETLISDFVNQSQDLQVSCAAPNQPSDQSQDLQMSCAAPNQPSNQSQDLQMSCAAPNQPSPVPAEANKENDKKKPRRSLQNAPTPEEWAQFVECYEKLPYDASKADYTFQVCREIEQEYWRTIGNGGQPMYGADTMGSLFDERTKDWNVANLDNLLTRLKLKKKIPGVNTPYLYFGTWRATFAWHVEDADLYSINYIHFGAPKFWYSIPQEHNPRFENFMSSSFAKERRTCSQFLRHKAFLASPSVLSSVGVQLNKVVHLPGEIILTYPYGYHSGFNLGYNCAESVNFANEAWIEKGRKAQSCKCIDDAVTINVDAWIAEHDELCRKQSETEARTAEKLRRQQKREERKRKGSENVDVKAPKKRQKKENNKATTKPSVGKIKVTLPAPLNPLIDPSLANPTPQKVKAPRKKKKPANSNPSNPPLPEKKDGDHQPVVVFENNQQTSSAPQLPMIPQYETGVFQNNLQGSSAPQLPTIPRYEPGVFQTNQQTSNAPQLPNIPYFGPDAFANNHQSSDAHQFPNISYFADQKFLSAVEAGLIDSRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>E3K984</accession>
    <name>E3K984_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">T-complex protein 1 subunit delta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_07286</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP81034.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325453.2">
        <property type="nucleotide sequence ID" value="XM_003325405.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K984"/>
    <dbReference type="FunCoup" id="E3K984">
        <property type="interactions" value="751"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K984"/>
    <dbReference type="GeneID" id="10536273"/>
    <dbReference type="KEGG" id="pgr:PGTG_07286"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07286"/>
    <dbReference type="eggNOG" id="KOG0358">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008891_9_1_1"/>
    <dbReference type="InParanoid" id="E3K984"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03338">
        <property type="entry name" value="TCP1_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000010">
        <property type="entry name" value="T-complex protein 1 subunit delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012717">
        <property type="entry name" value="Chap_CCT_delta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02342">
        <property type="entry name" value="chap_CCT_delta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP81034.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="543" mass="58082" checksum="3E62103795CB33F7" modified="2012-04-18" version="2">MAIPLGAPAAPNPGNSSNGPSQGRKAFKNAEKPMEVRLSNMTAAKAISDAIRTSLGPRGMDKMIQTADGNVTISNDGATILKSMSVMHPAARMLVELSAAQDVSAGDGTTSVVVLAGSLLGAAEKLLAKGIHPTIIAESFSRAAMKSVEFLEEISTPVDLNDRDSLLRAASTSLNSKIVSQYSSILAPIAVDAVKRVADLETSSVDLKNIRIIKKVGGTIEDTSLVDGMVLRQSVTNSQGGPSRKEKAKIGLIQFQLGTPKPDMDNEINVTRYGQLDKILKEERQYLLEICKTISKTGCNVLLIQKSILRDALNEIAAFFLAKFKIMVIKDVERDEIDFISKTLGCKPIADIEAFSADKLGEADLVEELSLNGAKTVRVSGIKNQGKTASILCTAANELVVDESERSLHDALCVIRCLVKKRALIVGGGAPEIHISRLLSQHAQTLKGMEAYCFQAFADALEVIPTTLAENAGLPNPVAVITELRSRHAAGERTAGINVRKGLISNMLEENVLQPLLVSTTAITLATETVGLLLRIDDYHPTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KA31</accession>
    <name>E3KA31_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07265</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP81013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325432.1">
        <property type="nucleotide sequence ID" value="XM_003325384.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KA31"/>
    <dbReference type="GeneID" id="10536252"/>
    <dbReference type="KEGG" id="pgr:PGTG_07265"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07265"/>
    <dbReference type="HOGENOM" id="CLU_150829_1_0_1"/>
    <dbReference type="InParanoid" id="E3KA31"/>
    <dbReference type="OrthoDB" id="2507116at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173680" description="Secreted protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="12705" checksum="DF253A14FFBDFF91" modified="2011-01-11" version="1">MNCLFYAACFLALLQSALAMPTLATRADQVAASHKGQSDEKCYFGCGFSNYGLYGSPFGSSYLTNYMSPYFYGNNLYGGLNYNLAYNSYGSNFGIGANLGLFKKDASEANKHQENSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3KA50</accession>
    <name>E3KA50_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_07284</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP81032.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325451.2">
        <property type="nucleotide sequence ID" value="XM_003325403.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KA50"/>
    <dbReference type="STRING" id="418459.E3KA50"/>
    <dbReference type="GeneID" id="10536271"/>
    <dbReference type="KEGG" id="pgr:PGTG_07284"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07284"/>
    <dbReference type="eggNOG" id="KOG1286">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1390847_0_0_1"/>
    <dbReference type="InParanoid" id="E3KA50"/>
    <dbReference type="OrthoDB" id="2268893at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1740.10">
        <property type="entry name" value="Amino acid/polyamine transporter I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004841">
        <property type="entry name" value="AA-permease/SLC12A_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050524">
        <property type="entry name" value="APC_YAT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341">
        <property type="entry name" value="AMINO ACID PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43341:SF4">
        <property type="entry name" value="ARGININE PERMEASE CAN1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00324">
        <property type="entry name" value="AA_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid permease/ SLC12A" evidence="6">
        <location>
            <begin position="146"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP81032.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="196" mass="21055" checksum="1EA8C225A07D076E" modified="2012-04-18" version="2">MPMLKLENGQDKGVTPAGGHAMYNKFIPHPMPKQISSSSEGREGTLSRKKALKNYAVFGSIPKCVCNAVFGSIRACPTASAYQMTMIARPLQYDTEPHKDNPRSRENLAVNVDILPFEEKKEDDGMSSNGSADASAQVQRKLKQRHISMIAVAGTIGTGLFLGSGQSLRQAGPIGTLLGYILMGAVVFSMQVALVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAF7</accession>
    <name>E3KAF7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07008</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81387.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325806.2">
        <property type="nucleotide sequence ID" value="XM_003325758.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KAF7"/>
    <dbReference type="GeneID" id="10537041"/>
    <dbReference type="KEGG" id="pgr:PGTG_07008"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07008"/>
    <dbReference type="HOGENOM" id="CLU_1696372_0_0_1"/>
    <dbReference type="InParanoid" id="E3KAF7"/>
    <dbReference type="OrthoDB" id="10398966at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81387.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="155" mass="17324" checksum="77125FBF0F590B43" modified="2012-04-18" version="2">MQEVEEIRGGCRWVDPYLIASPASSCSSVARHAGLPKLATIPLGASPVIVQFTLRSSTMCGRQLYEDRHFSVYMTEQEMRKVLFINETPKLNAKRCPGVQNSSNAKDYNCANSVPVDPTAKLGMPQVPRRRSSSAQDDHKHRYILYKPFSIALPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAL7</accession>
    <name>E3KAL7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_07050</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81429.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325848.2">
        <property type="nucleotide sequence ID" value="XM_003325800.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KAL7"/>
    <dbReference type="STRING" id="418459.E3KAL7"/>
    <dbReference type="GeneID" id="10537086"/>
    <dbReference type="KEGG" id="pgr:PGTG_07050"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07050"/>
    <dbReference type="HOGENOM" id="CLU_549969_0_0_1"/>
    <dbReference type="InParanoid" id="E3KAL7"/>
    <dbReference type="OrthoDB" id="2498368at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050613">
        <property type="entry name" value="Sec_Metabolite_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001">
        <property type="entry name" value="UNCHARACTERIZED TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31001:SF81">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="4">
        <location>
            <begin position="65"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="467"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP81429.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="496" mass="56072" checksum="0442A93C0F05BC55" modified="2012-04-18" version="2">MVGEAKEDYQKRLKAWRFSFSNKLASCTVHSLKLASYLGHPSIECIQAQLLLVLYMVNNDRCTDAWCFIGGVIKQAQCLGLHIDPSSLNPHMPVLEQELRRRVWWSVQTWDVFLGIAFGWPAGVTLSDSDLPSDRTEESLMGQALATTPILPPQGVTELTYHVFNWETCLYARDMMDRIFGQAVWGWGKSTEPQNGPKYEEVVKLDTTIKTWYHRVPVAMRFEPDPIEEYPAKQSGSFQTHSKVFKPSSTGVGIEDLRRREPMLAKQALLLSISQNTILLLLHRPFLSLSLENSTGATGHQMSEEQCVRSSQVIIEAQRLLVELFPATRRMWYGWYLTFHAAMTCAWISLLRTPTHPMSGLSRKCITLGIETFELAIKTPESLPENYLRACSQLRAIRNYSNRKLNPPKAITYSNHVFGATGSIPSFAYSLAPRTMTTLARNIDKYPNFAEPSSRLDLGNEQDMVLFEDPTISSQSLTSNPSSYPKESSSHHEEAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3KB04</accession>
    <name>E3KB04_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGTG_07796</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the pacC/RIM101 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81547.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325966.2">
        <property type="nucleotide sequence ID" value="XM_003325918.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KB04"/>
    <dbReference type="STRING" id="418459.E3KB04"/>
    <dbReference type="GeneID" id="10532928"/>
    <dbReference type="KEGG" id="pgr:PGTG_07796"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07796"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_302881_0_0_1"/>
    <dbReference type="InParanoid" id="E3KB04"/>
    <dbReference type="OrthoDB" id="2507583at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000213">
        <property type="entry name" value="Zinc finger protein 624"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050806">
        <property type="entry name" value="pacC/RIM101"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47257">
        <property type="entry name" value="PH-RESPONSE TRANSCRIPTION FACTOR PACC/RIM101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47257:SF1">
        <property type="entry name" value="PH-RESPONSE TRANSCRIPTION FACTOR PACC_RIM101"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0678">Repressor</keyword>
    <keyword evidence="9" id="KW-0804">Transcription</keyword>
    <keyword evidence="7" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 12" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="20"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="58"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="14">
        <location>
            <begin position="88"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="143"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="311"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="432"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="631"/>
            <end position="659"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="672"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="744"/>
            <end position="763"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="774"/>
            <end position="846"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="146"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="311"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="432"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="697"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="744"/>
            <end position="756"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="774"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="805"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="828"/>
            <end position="839"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EFP81547.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="984" mass="106513" checksum="46B9DA8D99641082" modified="2012-04-18" version="2">MTHILPAGAPHNGTASAQVLQCEWQEPACSELFPDPEALYRHLCDRHIGRKSTGNLTLRCHWKNCETVCVKRDHITSHLRVHTPLKPHNCDVCGKAFKRPQDLKKHEKIHTEEHHIHHKHSKAAKAPAFYPVNYSNRPYAPLPQHAQAQQSHTMSSSTSPFDFNNSQNSNQFSWPSVRHLVQQQQDMNESVVIHGLAQAAAQQAYHSVLASSTVSSQNLMGGVQSVTGPIFPNAVSYPTPMPSATTTHMSMTNNPLAPVSSVDCSRQFASSGADLSYPSLQMLSSRPQNTSISGASIGSITDCLSDQGLSNFSQDPGPYVSQSTRTPPHPSYARSSLSSPPHHPLSPPGAASDITRSQTSDDLFQSLCAPRHSFSAQPPPPVGGHKRDYDEAVYDLISRCKRSKFEEDSDETIARLAKFLCPDVNLNLPSLDSHSSSDASSPVSSPHPYPAEQSSTLSPPSDLDWLADKAETDKLTDLLAHIGSEIESSTAAIFGPSWPDILSFNTSLPTEQPAVKRTSTTSLSSAPLNHHIPNAELDRTSSFHTARHPHNPSYGSMPPVTSSSNYPTLPTSYCGYPNLQRPPADCQMRVSKPLAPPELSSASYYSAPMSLHKVNPLQRAMPVATHAPTNMESALQPSGQKLADQVDFSNDGADSSSRRLSIDQSLELAPMGLSKSKPLSGTTLPPLRTLFGDSDRGSSPASAVSSPGSPAQFSSFVPAGRSASSGPKSIYPSLMNLTATFGRSAEDKTASERTETADSLVPSVRGMNLASRRASVASIRSSSSPAVPEEWVNEIGEEPSPREEGDSDSEEGGEGGESLPHHKRFRLSPRSTTRSTPSLAGSAAQSLVHNSLSGNDKDTLDHTGKQLSLIHSLIIRINEAHRQTIIARSAARLAPAYPYYPSPSQFAARKEGSLPLVDDDNDDNSPTAPKSLMSYELPLCFPPPPLTILSFCFFQTSDRLPCFVPSRMMLFRSFFSSLLFFFFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3KB48</accession>
    <name>E3KB48_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Beta-1,3-glucuronyltransferase 2 (Glucuronosyltransferase S)</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_07817</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the glycosyltransferase 43 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81568.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325987.2">
        <property type="nucleotide sequence ID" value="XM_003325939.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KB48"/>
    <dbReference type="GeneID" id="10532949"/>
    <dbReference type="KEGG" id="pgr:PGTG_07817"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07817"/>
    <dbReference type="eggNOG" id="KOG1476">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_514971_0_0_1"/>
    <dbReference type="InParanoid" id="E3KB48"/>
    <dbReference type="OrthoDB" id="675023at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015018">
        <property type="term" value="F:galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050650">
        <property type="term" value="P:chondroitin sulfate proteoglycan biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005027">
        <property type="entry name" value="Glyco_trans_43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10896:SF65">
        <property type="entry name" value="GALACTOSYLGALACTOSYLXYLOSYLPROTEIN 3-BETA-GLUCURONOSYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10896">
        <property type="entry name" value="GALACTOSYLGALACTOSYLXYLOSYLPROTEIN 3-BETA-GLUCURONOSYLTRANSFERASE BETA-1,3-GLUCURONYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03360">
        <property type="entry name" value="Glyco_transf_43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="94"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="105"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="12">
        <location>
            <begin position="152"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="9">
        <location>
            <position position="485"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="396"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with galactose moiety of substrate glycoprotein" evidence="11">
        <location>
            <position position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR605027-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR605027-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR605027-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EFP81568.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="16">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="529" mass="58626" checksum="7627CE84E4D5AAA1" modified="2012-04-18" version="2">MLGSAQCDPQDKPYTLLPTQNSISEQPMHHSSLCLLTRLKSIFRTRFLLFQLILILCLVLTLLRFYPFSDHLSRHGKAYSLTFSIDSINPTRSAAVSQSVSQPVSHPPSQPASHPPSQPASPAPSPPTPSFPQPQPPLLPPAITKAGSRPAVPRPVVPHPLRPLPPPPPTSAALCARCGCSSDSRIRREFNPPDELVIQRSPNATISIDSVDQSSAVITQIWSSIRDVYCQHPLLASEAALTLLDRSVPDSSVLPATISTRASTLQVGLPTQYIFTKTSALGRTGGLRPDYFRRHVKAIRDHLSTVARRGGKYLEETEKKWGTGRRQLIWIVIEDGESIAADIQQVLSQSGLPFIYFAYGPTRHHGNAQQNSAYALIHRLSRTILGHGPILSIDDDGKVLSEVFDIAWRVHTFGVWPMGNLGPSGWEGPEYDPVTLEFLTWRQAPEDDRPFPLDNGAFVFSSEVFGTNFRLVGPRYWPTDYPGGESEFVSQIISKQELVEPLCYNCRVAWHNQKLPEDCIKHLPSCKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBF4</accession>
    <name>E3KBF4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_07915</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326085.1">
        <property type="nucleotide sequence ID" value="XM_003326037.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KBF4"/>
    <dbReference type="GeneID" id="10533211"/>
    <dbReference type="KEGG" id="pgr:PGTG_07915"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07915"/>
    <dbReference type="eggNOG" id="ENOG502QQG9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011406_0_1_1"/>
    <dbReference type="InParanoid" id="E3KBF4"/>
    <dbReference type="OMA" id="WIISFPP"/>
    <dbReference type="OrthoDB" id="2504919at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015693">
        <property type="term" value="P:magnesium ion transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008521">
        <property type="entry name" value="Mg_trans_NIPA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12570:SF86">
        <property type="entry name" value="ADR321CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05653">
        <property type="entry name" value="Mg_trans_NIPA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="341"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="383"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="414"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="441"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="195"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="283"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="472"/>
            <end position="544"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="605"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="199"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="223"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="234"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="255"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="289"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="305"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="512"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="613"/>
            <end position="623"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="633"/>
            <end position="661"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="680"/>
            <end position="695"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP81666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="704" mass="78243" checksum="94C6D1FBC66E4DE7" modified="2011-01-11" version="1">MNNQDHQPPFNLALGISIGLLASFIQSLGLTIQRKSHLQNDRLPPSSRKPDYARPVWLIGFTIYFSFNILGSIFQIGTLPLIILGPLGAISLLWNAILARLLLADRFSLHLIFGTILIGLGALLIGLYGLLPDQSASHDLDQLIYLYSRTPFLVEIIILILTFSIICSFAHLYEFRLNQSLETISLLTISPTPTPPDPSNLNLNNRLPNQTNDPPHSRHSLNLIPSQSITSHGSLRKKKIHHHHHHHYPAYRPRSLSSPTHQNHPLSLSFPHQPIDIIDSHIPLTPPFSKKRHQRPSRPSRTLKRANSTYSSGPVQLSIDKDETGGCQGLLSSQKIDKLKLLIGIAYGSTSGTLSGICLLFAKTGVELLIRSLTGAVNEFYKFQTWLILLVLLISALLQLWYLNKALRLVNPTLICPLAFCFYNLSSIISSLVYYDQVKLLSAFQIFAISLGTIILLGGVWVVSIGTNKTSSKHTSEEEEGLEEDGRSSHGSVVDEDEVTPLLPSSSRIGGMDDDGDGEEPIVCTEDPEGIDDSFDSPDRAAHSYSHRNALLQSSSGSSSPINPRRSSHPFDTLIRQFLVEGEVTPVRGFSIGLGAASPGFAIRPKKRRKNTENVGRSSLTDWRSNDHLDDCQQYQQQQQQQQRQHQNQNEHQQYESQDQQQQEDHLPQVQQQPPRLSIGTDTNRLITPDTCQPFSSSASSCSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBX2</accession>
    <name>E3KBX2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08023</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EFP81774.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326193.2">
        <property type="nucleotide sequence ID" value="XM_003326145.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KBX2"/>
    <dbReference type="STRING" id="418459.E3KBX2"/>
    <dbReference type="GeneID" id="10533639"/>
    <dbReference type="KEGG" id="pgr:PGTG_08023"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08023"/>
    <dbReference type="HOGENOM" id="CLU_401771_0_0_1"/>
    <dbReference type="InParanoid" id="E3KBX2"/>
    <dbReference type="OrthoDB" id="2507845at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="189"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="601"/>
            <end position="685"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="256"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="315"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="351"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="388"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="610"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="647"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="669"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81774.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="685" mass="73749" checksum="CE95F6551E8EC989" modified="2012-04-18" version="2">MKLDTHGIALFQVKKSSKAPNHTIDVDQSGTDDTSDHEYRYRTRSGRRPPPSPAYPALDDLSYAEEGRQDVNSYPRPKISPFLQLPPERNLSEKDNAPTPVFNPYNPCPKRRRYVQLQGGNQTYAAPGLDHRDRHGPDPSDWRFTPASIPFCPTRAETSNPPSQIQTQLAPSYGYDTFQQPPVFQQPSVFHNTNTSQSDFARPYAYQPPATHFDYSPRRFTDHTYTPHLNPHQPSGPAACPKPEVPSGPKPFDYSQYWNTRPTQSQKQNVSFSQGSAVPSQKDSSGFNFSCPSVGQEKPPPPPPNRAQEKVQSLPRFSTQSCPFGTQEKAPPPPKYGQSSSLNLPPARFGQFSSVTTFKPSTVEPTRNNPFMPDASSWDPKPPPVPPKSDVSTNLWGSKPSTFQSFQSSTGSTSTLTSFSTPKPPTTSFATPKPPPQHPQHTSYRAECKSQKTSPAYNFKVPSPPTSPTLAAPGDSNMDDLCNAISRLKIIRPVVPIPRKNSGPNSKKVRFADVSLMNAILAEFLGEAAGHQIDPQLFSGQTAHIPSSTAPSTAAALSSTVAALPSTAAAPALTTATPLPTISTTVAPAALPAPSLTTVALPAPTLTAGPPTPASVQPRPPGGTPQPSVQPRPPGGTPQPPIPNLTIARSNLTIASSSNLTPNEPTSKPSKEDGETPPSEKEKSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCQ0</accession>
    <name>E3KCQ0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_07599</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326621.1">
        <property type="nucleotide sequence ID" value="XM_003326573.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCQ0"/>
    <dbReference type="GeneID" id="10529574"/>
    <dbReference type="KEGG" id="pgr:PGTG_07599"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07599"/>
    <dbReference type="HOGENOM" id="CLU_877547_0_0_1"/>
    <dbReference type="InParanoid" id="E3KCQ0"/>
    <dbReference type="OrthoDB" id="10319415at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP82202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="317" mass="37398" checksum="4B786EBDEEB2A427" modified="2011-01-11" version="1">MSGALPDECIPLLERNSQGEKLRDESTSGDAESLKDGVVPRIDISEDSMRNLAIHVDVIAPQNTLSEDEASIARHLGNFFRFYKEETSIPIYNDNAILNDNKFIKKNLESWRFSFWKIGWNKDEKKAAKKLSEQHEKLVDFRSATSPYRFFKVEQKKSYDGAIESIGFHKHGIYIYFSDLDYPWFGFKSFCKNNPIGDNEFQYSLVKFLDRMLHDTPSFFIDRKIDLKNEVNRKSLKLQRIIFQLVDYMLKYQLIKMDHYNDFFRDEKRLELAAINMVATFGLDNQKDLEFKFNKYYSSKIFLRTWNCSHLKSLPES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>E3KD96</accession>
    <name>E3KD96_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">acetylornithine transaminase</fullName>
            <ecNumber evidence="5">2.6.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_07707</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 4/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.11" evidence="5"/>
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        <property type="protein sequence ID" value="EFP82310.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326729.2">
        <property type="nucleotide sequence ID" value="XM_003326681.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KD96"/>
    <dbReference type="FunCoup" id="E3KD96">
        <property type="interactions" value="143"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KD96"/>
    <dbReference type="GeneID" id="10529683"/>
    <dbReference type="KEGG" id="pgr:PGTG_07707"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07707"/>
    <dbReference type="eggNOG" id="KOG1401">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_10_1_1"/>
    <dbReference type="InParanoid" id="E3KD96"/>
    <dbReference type="OrthoDB" id="10260828at2759"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00109"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003992">
        <property type="term" value="F:N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000004">
        <property type="entry name" value="Acetylornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004636">
        <property type="entry name" value="AcOrn/SuccOrn_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050103">
        <property type="entry name" value="Class-III_PLP-dep_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00707">
        <property type="entry name" value="argD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF79">
        <property type="entry name" value="ACETYLORNITHINE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986">
        <property type="entry name" value="AMINOTRANSFERASE CLASS III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="9 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP82310.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="484" mass="53129" checksum="1DE3E9CD51F6D510" modified="2012-04-18" version="2">MNTSRKITNPRLTRQLKQSYKLLHPTSIRFISHLTHPDHPVPQNVQHRIERWSKSMLTPYARPPMILSHGKGCKVWDTEGREYLDFTAGIAVNALGHADKEVAEIMYQQAQKLVHCSNLYHNDQAGELACTLVNLTKKFGGLGYPPLADSSSVSAHQESPQDHKVFFANSGAEANEGALKFARKCGKIEEPTGSKHELICFTNGFHGRTFGALSVTNQPKYQLPFAPLLPGIKPGNLNDISALTELITPNTCGVIVEPIQGEGGILEASEEFLRALRHRCDEVKAVLIYDEIQCGLGRTGNLWAHGSLPTDCHPDMLTMAKPLANGVPIGAVMMRDNVAKKIAIGDHGTTFGGAPLQTAIAKHVLERICKESFMDQVRGTSEVLKKELNRLVAEYPNILTGPVRGRGLIMGLEVKNLQSEDHAKQSEPTISQIINLSRQNGLLLLSCGQSIIRFVPSLIISHSEVSQMILILEKVLAHLSSPKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>E3KE74</accession>
    <name>E3KE74_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_09008</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327231.1">
        <property type="nucleotide sequence ID" value="XM_003327183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KE74"/>
    <dbReference type="GeneID" id="10544519"/>
    <dbReference type="KEGG" id="pgr:PGTG_09008"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09008"/>
    <dbReference type="HOGENOM" id="CLU_1540847_0_0_1"/>
    <dbReference type="InParanoid" id="E3KE74"/>
    <dbReference type="OrthoDB" id="10337703at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP82812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="174" mass="18903" checksum="1920A12C43C1E249" modified="2011-01-11" version="1">MPDGLVKAGRWMDGLTLQEFGKVLPNEACKQRNMFGLLLESHDQRRLRSSGGPLTLKSLQHSSSPSSPIAKIHVLHIEMYPLGFPLLSAFACHLPVEAPLSLSLLVGNENLLASDIQWNKVDPDNPIGYMSWKSAIDQLPAPKSSFLSTSSSSGDWTAGPAHFASQVCDSHTQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KFE2</accession>
    <name>E3KFE2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_09937</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178284">
        <property type="protein sequence ID" value="EFP82969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327388.1">
        <property type="nucleotide sequence ID" value="XM_003327340.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KFE2"/>
    <dbReference type="GeneID" id="10544680"/>
    <dbReference type="KEGG" id="pgr:PGTG_09937"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09937"/>
    <dbReference type="eggNOG" id="ENOG502S6K1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008138_0_0_1"/>
    <dbReference type="InParanoid" id="E3KFE2"/>
    <dbReference type="OMA" id="YICERHE"/>
    <dbReference type="OrthoDB" id="2496194at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266">
        <property type="entry name" value="CHROMOSOME 15, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266:SF1">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP82969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="858" mass="97414" checksum="EC554372318A4B31" modified="2011-01-11" version="1">MAPRRLPPPYHLRSKVTSLEERFKRLNVESEDKHFRKFSQSVPPRLVERYLEILEELFKHFRVAPGNLELGALTLKVATGVIILAWDKISSAFGAKENKSIEDKFVELAFLRRYPDYYENEQIDWEARASIFSVSLDQGRSILERATEPSTVAVDVVRKGYLSDYVGRDEIVTPILSTLNENASSWRPEEYHAPYTSLIGPTMIGKTRLLMELADEICVVYICLRLPNSSGEPKRSQLATEMLETPLGADLEVYYVQLITAILSVTIKFFQSASKRKDRKELFRAWYQHHNSPNTKFYSNVQSELKRLTGKNDAVHQLILAAEKLGKTHILKSSPLKVLLAIDEANTLLDKPKNRTVSSENQSEEPPLFRFFRRALRNVPDSSGFFAILVDTNSCVANFSPRTEDDPSCRSIGTRAEPFKLYPPIYELRTMDRMVPADPPRSWAELFLPERLCSYGVPFFGSYLKTKMRANLSVAVDKMARFALNKLLCSFKEGPIKITESRALALLGPTIGVPLHGQARLNSQLMASHAAHCGYIDANRDSQYAFYPSQPIYALAANYYLQTNEDVLISCINSLTAVLSQGDVGPGDAGEIASRIILLCAMNKTAADMKTAKETSADLIGVKHISFPDPVPVIKFLETLTGISAHELPLGSIDANHKRKLLEHGMMFWNHFMHFSERPTTESMLECLHRGLALQCRSNQEAFDQVLTIYLKDQFEDELDEANVTFCGIQVKNRKYDSELKNSQGKMNPEKAKIEIKEKTNPYLSLYFTLQNTPPKKKENYKRQDNYKLPSNGPPDYRQASLVFYGLDSFHFLSPGLKEALKQLIDIRTDLVSRHGKRKQGLDYVTDFFLRSTARRLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGL2</accession>
    <name>E3KGL2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable enoyl-CoA hydratase, mitochondrial</fullName>
            <ecNumber evidence="2">4.2.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_08623</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the enoyl-CoA hydratase/isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.17" evidence="2"/>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327856.1">
        <property type="nucleotide sequence ID" value="XM_003327808.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KGL2"/>
    <dbReference type="STRING" id="418459.E3KGL2"/>
    <dbReference type="GeneID" id="10532114"/>
    <dbReference type="KEGG" id="pgr:PGTG_08623"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08623"/>
    <dbReference type="eggNOG" id="KOG1680">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_7_6_1"/>
    <dbReference type="InParanoid" id="E3KGL2"/>
    <dbReference type="OMA" id="FCDARED"/>
    <dbReference type="OrthoDB" id="2018133at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004300">
        <property type="term" value="F:enoyl-CoA hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000019">
        <property type="entry name" value="Enoyl-CoA hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.12.10:FF:000001">
        <property type="entry name" value="Probable enoyl-CoA hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.10">
        <property type="entry name" value="Lyase 2-enoyl-coa Hydratase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018376">
        <property type="entry name" value="Enoyl-CoA_hyd/isom_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014748">
        <property type="entry name" value="Enoyl-CoA_hydra_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF54">
        <property type="entry name" value="ENOYL-COA HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00166">
        <property type="entry name" value="ENOYL_COA_HYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="4" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00073937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP83437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="291" mass="31648" checksum="9971F629D979F2EA" modified="2011-01-11" version="1">MMMMTIRRTASFLSPRSLTLARRFQSSQTPSYQNIIYSNPSPAVGLLTLNRPKALNALSSPLFTEINDLLSSINSSSSSVKCLVLTGSEKSFAAGADITEMAERSLAEVYDADFLKFWSEKISSFRVPIVAAVSGYALGGGCELAMMCDIILASKDAKFGQPEINLGVIPGAGGTQRLVKAIGKSKAMEYILTGKTFSAQQAYEWGLISRVVDGGHEELLKESLALADTISTKPKLATKAAKEAVNLAYELPLSQGLEFEKRLFHLLFGTNDQKEGMKAFVEKRKPKFTHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHR2</accession>
    <name>E3KHR2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EFP83837.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328256.2">
        <property type="nucleotide sequence ID" value="XM_003328208.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHR2"/>
    <dbReference type="GeneID" id="10532519"/>
    <dbReference type="KEGG" id="pgr:PGTG_09550"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09550"/>
    <dbReference type="HOGENOM" id="CLU_1949875_0_0_1"/>
    <dbReference type="InParanoid" id="E3KHR2"/>
    <dbReference type="OrthoDB" id="10287726at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83837.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="129" mass="14696" checksum="662327406E492870" modified="2012-04-18" version="2">MSRFNYVLVSQTKKIGICTPVGPGEFLSDEIKGGRDANDLPRFNSKDSCKPQGDSKQLEDWDSMIKSVKNMENDYVKTKMHEFEYSDCNIIKESIDELNSCHNQIEHVFGSPTLRKTWAHPGIQEARAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIA7</accession>
    <name>E3KIA7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09745</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EFP84032.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328451.2">
        <property type="nucleotide sequence ID" value="XM_003328403.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIA7"/>
    <dbReference type="GeneID" id="10532727"/>
    <dbReference type="KEGG" id="pgr:PGTG_09745"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09745"/>
    <dbReference type="HOGENOM" id="CLU_1769035_0_0_1"/>
    <dbReference type="InParanoid" id="E3KIA7"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84032.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="147" mass="16148" checksum="D69E318362FA5755" modified="2012-04-18" version="2">MRAPPVTPTPTSRQCTISKQGPRTPSTSPGTPPTRSISASPLQAKKVPRNARSTPSPIKRHDAITNNEPGDSEKRQCVEKTELAFVHKAPIKPNPQKPVTGIRPLLETSMTGVAPKTPAKPNVNTVVQLFCLEVIWEWFPECKPQHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIG7</accession>
    <name>E3KIG7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_10470</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84092.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003328463.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIG7"/>
    <dbReference type="GeneID" id="10545190"/>
    <dbReference type="KEGG" id="pgr:PGTG_10470"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10470"/>
    <dbReference type="HOGENOM" id="CLU_960216_0_0_1"/>
    <dbReference type="InParanoid" id="E3KIG7"/>
    <dbReference type="OrthoDB" id="4748970at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="3">
        <location>
            <begin position="186"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="202"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="258"/>
            <end position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP84092.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="290" mass="32364" checksum="42B8AC4F351167C5" modified="2012-04-18" version="2">MQKIVIPKGLEIVKTPSFYFPLKAKWHSIYDPQGHYDLSSIPTTVPRMNSHNNPHPFVCVAWNVNDPGVENLLLNNNPPPTAVCLYTEEDAMRIFEAIQSTSYHAGVTLPLGSQLPLGNVGLEVLPAGQESPAQDTSGPAPVYIRLPSLEYHCECGAIVKKNRKHNMIRHINTVHRTPGDPQRPLYECDKCGKTYMNSGELAQHKRDNHDAPPPKPRAMKQKAASEKFPCQVAGCEHEYASLESQAYYRHLQEKHGVEVERKLEEREMVPTSPPHNPIAGPSRPRGPPAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIN6</accession>
    <name>E3KIN6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_10539</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84161.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328580.2">
        <property type="nucleotide sequence ID" value="XM_003328532.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIN6"/>
    <dbReference type="GeneID" id="10545339"/>
    <dbReference type="KEGG" id="pgr:PGTG_10539"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10539"/>
    <dbReference type="HOGENOM" id="CLU_659116_0_0_1"/>
    <dbReference type="InParanoid" id="E3KIN6"/>
    <dbReference type="OrthoDB" id="2515958at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173918" evidence="2">
        <location>
            <begin position="24"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="92"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="209"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="25"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP84161.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="417" mass="42632" checksum="54E9974924206143" modified="2012-04-18" version="2">MQARKTLFSFVLVVSALAGLASAAPSSSPADAAAAAPPAQEKAGSPAAAASAASADKQTAAGQEGASTASQANPQNAAVFAATGAADATTAATTGEDASAKADHKKRNADAAPTGKIPDSADKADSTTQANPQNAAVFSAISPTDASTVSTAGDEAFSNTGHEKRNADAPPAGKIPESADKADSGAQGVPQFNAVDAAANGQATDAAFASTHAVAQTRRSEPSGAAADGKKDATAENTSQPSPKDSQTPQEAQQLNTSPSGSTPDSGAENPASDKKDHWHFLSRAKTGPAVPVSQGRLRGSGFLILWVDAMVGVELSQTQRRKKQKGSVRRVLVMIPRFRSALHLALNSCCRLLLKPGSAYRSYPGALAASAAQVRLSIVERFKWTSGSRRTTRLPIASARHLLHEPHPFPSHLLPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIQ1</accession>
    <name>E3KIQ1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_10554</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84176.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328595.2">
        <property type="nucleotide sequence ID" value="XM_003328547.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIQ1"/>
    <dbReference type="GeneID" id="10545354"/>
    <dbReference type="KEGG" id="pgr:PGTG_10554"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10554"/>
    <dbReference type="eggNOG" id="ENOG502RY0B">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036832_0_0_1"/>
    <dbReference type="InParanoid" id="E3KIQ1"/>
    <dbReference type="OrthoDB" id="2448307at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040410">
        <property type="entry name" value="UPF0658_Golgi"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34391:SF2">
        <property type="entry name" value="TRP C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34391">
        <property type="entry name" value="UPF0658 GOLGI APPARATUS MEMBRANE PROTEIN C1952.10C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="137"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="184"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="248"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="288"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="320"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="418"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="346"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="361"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="448"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP84176.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="497" mass="56296" checksum="1B827330FDFCDFE8" modified="2012-04-18" version="2">MKSSRWTQTIKPTINQFLPQSVYGKTFLIITILESCIDVILESVVIARLNLIHTFKLSFDSAATKTPLAVYLGIFILAHLFQLYFALDALRHKNTIQLIGLCCFNFAFLVYAIIQVPEIRKIESVEGSNSTNSNLTPAIILSIIPACIGISQVAFVYLTWHLFKEFGWQIYKQIGADRKIKRVYLWYQIFMCLLKFDYFFFMAFTVQLVLLVPSVSPLERVLTIIALPTTFLFLVIGYYGVRKEIKSVTCLFMLGLLSGSCYFVYKLFRIWQGRSNVLSYANVFKSLTVFSVSCLLMTLLSFTSAIICLLNFGKGLKPQIDKTSSTRKKPSERRRQAERVYGNTGEGRRQHDEQGGERYSRTGSGTTLGTMTKQGGLEVYMDGGMEQQKLSTFKSNNHQHGFLEDPVGGYFSSHLDFDDDDRLRSSPPTLPPPPFELSAPNHHLLNLSFPQSTATPTTTLSQARTTHQPHGFLHSHSQRRQNSASINDDSQKRMSID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJ13</accession>
    <name>E3KJ13_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 11">Chitin synthase</fullName>
            <ecNumber evidence="2 11">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_10666</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">Polymerizes chitin, a structural polymer of the cell wall and septum, by transferring the sugar moiety of UDP-GlcNAc to the non-reducing end of the growing chitin polymer.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>[(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n) + UDP-N-acetyl-alpha-D-glucosamine = [(1-&gt;4)-N-acetyl-beta-D-glucosaminyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16637"/>
            <dbReference type="Rhea" id="RHEA-COMP:9593"/>
            <dbReference type="Rhea" id="RHEA-COMP:9595"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17029"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="EC" id="2.4.1.16"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cell membrane</location>
            <topology evidence="1 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the chitin synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2 11"/>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328707.1">
        <property type="nucleotide sequence ID" value="XM_003328659.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJ13"/>
    <dbReference type="FunCoup" id="E3KJ13">
        <property type="interactions" value="33"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KJ13"/>
    <dbReference type="GeneID" id="10545632"/>
    <dbReference type="KEGG" id="pgr:PGTG_10666"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10666"/>
    <dbReference type="eggNOG" id="KOG2571">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004760_3_1_1"/>
    <dbReference type="InParanoid" id="E3KJ13"/>
    <dbReference type="OMA" id="AWILHYV"/>
    <dbReference type="OrthoDB" id="26569at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04190">
        <property type="entry name" value="Chitin_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013616">
        <property type="entry name" value="Chitin_synth_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF9">
        <property type="entry name" value="CHITIN SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01644">
        <property type="entry name" value="Chitin_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08407">
        <property type="entry name" value="Chitin_synth_1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="4 11" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="592"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="631"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="661"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="713"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="744"/>
            <end position="761"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="842"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="880"/>
            <end position="904"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin synthase N-terminal" evidence="13">
        <location>
            <begin position="167"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="78"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="78"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024009"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU366040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF08407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EFP84288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="910" mass="102932" checksum="F45B6ADC7A804151" modified="2011-01-11" version="1">MAYHQPVDEERGGTWGMPNHQQTGHDIPLRDPLQSPQAFVPPTHVINDPYYPSQNYQQSEYLEPTVYGGGGLHEDYDSSSWVPASQHPSSNYESSISGTHTYEPKISSGFEHPVPSDAHEDVPLIRNPGEYHQNNNYTPGGFSAEVPTEDSYVRYGRIPQRQPRRYKTVKRVELYHGNLVLDCKVPTKLLAMCPRKDEREFTHMRYTAATCDPNDFKDERYTLRQVLYEPARRTELFIVMTMYNEDDQLFTRTMHGVMKNVQHLCSRDRSKTWGKDGWKKVVVCIVSDGRSKINSRTLSVLAAMGVYQDGVAKNVVNDKPVTAHIYEYTTQISIDPDLKFKGSEKGLVPVQILFCLKEKNQKKINSHRWFFNAFGPILQPNVCVLLDVGTQPGVSSIYRLWKSFDINSNVGGACGEIVALKGKFLRNLLNPLVAAQNFEYKMSNILDKPLESVFGYITVLPGAFSAYRYIALQNDAKGEGPLTQYFKGETLGHSADADLWTKNMYLAEDRILCWELVSKRNSSWILHYVKNSFAVTDVPDQVPELISQRRRWLNGSFFAAIHSTVHFGYLYRSSHSFLRKAVLHFEMVYQTFSMLFSWFGLANYYIAFVILTKSMEDPSFNFNGIQYFNDVLRYIYLGVLIMCFLLAMGNRPQGSKVMFTLALVVFAFITLYMTFAGVFIAVKGILNAEAAIVASGGHFTVADIFANRIFRNIVLSLVATFGLWLIASFMFFEPWHMFTSFAQYILMSPSFINVINVYAFCNVHDVSWGTKGDNKVETDLGVVKAAEDGKTGKVDITVPTDEKDINAAYDDACHVLSNKPPPEKETIDAETKMTDSYRNIRTNVVLAWALMNGALVAVILSTKAGSNISQDGDSAKVNTYMTFLLYSVFGLSFVKFVGTTMYLVQFIFTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>E3KP72</accession>
    <name>E3KP72_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Obg-like ATPase 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_12053</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for GTP.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
            <location evidence="2">Cytosol</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. YchF/OLA1 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178298">
        <property type="protein sequence ID" value="EFP86097.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330516.2">
        <property type="nucleotide sequence ID" value="XM_003330468.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KP72"/>
    <dbReference type="FunCoup" id="E3KP72">
        <property type="interactions" value="496"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KP72"/>
    <dbReference type="GeneID" id="10540103"/>
    <dbReference type="KEGG" id="pgr:PGTG_12053"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12053"/>
    <dbReference type="eggNOG" id="KOG1491">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018395_1_2_1"/>
    <dbReference type="InParanoid" id="E3KP72"/>
    <dbReference type="OrthoDB" id="424823at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006950">
        <property type="term" value="P:response to stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04867">
        <property type="entry name" value="TGS_YchF_OLA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01900">
        <property type="entry name" value="YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.300:FF:000003">
        <property type="entry name" value="Obg-like ATPase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000001">
        <property type="entry name" value="Ribosome-binding ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.300">
        <property type="entry name" value="TGS-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00944">
        <property type="entry name" value="YchF_OLA1_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004396">
        <property type="entry name" value="ATPase_YchF/OLA1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023192">
        <property type="entry name" value="TGS-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013029">
        <property type="entry name" value="YchF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041706">
        <property type="entry name" value="YchF_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00092">
        <property type="entry name" value="redox-regulated ATPase YchF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305">
        <property type="entry name" value="OBG GTPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305:SF11">
        <property type="entry name" value="OBG-LIKE ATPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06071">
        <property type="entry name" value="YchF-GTPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="10">
        <location>
            <begin position="80"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="89"/>
            <end position="94"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP86097.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="454" mass="50184" checksum="56666C6547F43444" modified="2012-04-18" version="2">MPPSLLLLFPSQLLPRVTVQPIRPSLSLTTNPSHTFQSLFSTSSAQSISPTSHLSIKKRMPPKKTVVEKKALLGRPSNNLKIGIVGMPNVGKSSLFNVIAKCDLGKSANFPYATIDPEEARVPVPDERFDWLCSVYKPTNKIPAFLTCIDIAGLTAGASTGAGLGNAFLSHVRAVDGIFQVVRAFDDAEVIHVEGDVDPIRDMEIIHTELKLKDIEWVEKHYETIKKSFRGTGTANLADKAKKEEIDITGKVLAWLKDEGRDVRKGDWNNKEIDVINTLQLLTAKPVTYLINLSETDYIRKKNKWLPKIKAWIDANNPGDLLIPFSVALEERLLQLGVDDEVPARKEELAKIGTTSALGKITTAGYASLHLIRYFTTGPTEVRAWTIRKGTKAPQAAGVIHSDFENKFVCGEIMTYGDLKEHGSEVAVKAAGKYRQQGKPYEMQDGDIAFWKHG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3KP92</accession>
    <name>E3KP92_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12073</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178298">
        <property type="protein sequence ID" value="EFP86117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330536.1">
        <property type="nucleotide sequence ID" value="XM_003330488.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KP92"/>
    <dbReference type="GeneID" id="10540125"/>
    <dbReference type="KEGG" id="pgr:PGTG_12073"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12073"/>
    <dbReference type="HOGENOM" id="CLU_1129542_0_0_1"/>
    <dbReference type="InParanoid" id="E3KP92"/>
    <dbReference type="OrthoDB" id="10394478at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015085">
        <property type="term" value="F:calcium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070588">
        <property type="term" value="P:calcium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032468">
        <property type="term" value="P:Golgi calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032472">
        <property type="term" value="P:Golgi calcium ion transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071421">
        <property type="term" value="P:manganese ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="197"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="237"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="246" mass="26867" checksum="E441787F6F00C1BF" modified="2011-01-11" version="1">MLRDQIQVGNATTLAPSQRQQAPHDHLDQPAHTLFILPPISSSDQHPSSGSSAASMATRNRRTSFVDMSWMLPALGISSQTVTQSLMERWRDALRTGSQSIVKTPSPSQTLLVMSVLSAGLGHVLPTLISRRYTVLAALELFLVFGLKMIRDVRHNGYHGIGINSTAGTPTDTHPPSYFHDASPDLIGRPLSNYTGFRPDRTYFTPDGTDSNLGDRLTDSKGADGYPQQHGNVYAKSKKEAKDAKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQD8</accession>
    <name>E3KQD8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FAR-17a/AIG1-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_12469</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178300">
        <property type="protein sequence ID" value="EFP86513.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330932.2">
        <property type="nucleotide sequence ID" value="XM_003330884.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQD8"/>
    <dbReference type="FunCoup" id="E3KQD8">
        <property type="interactions" value="129"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KQD8"/>
    <dbReference type="GeneID" id="10540528"/>
    <dbReference type="KEGG" id="pgr:PGTG_12469"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12469"/>
    <dbReference type="HOGENOM" id="CLU_081915_1_0_1"/>
    <dbReference type="InParanoid" id="E3KQD8"/>
    <dbReference type="OrthoDB" id="2499708at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006838">
        <property type="entry name" value="ADTRP_AIG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10989">
        <property type="entry name" value="ANDROGEN-INDUCED PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10989:SF16">
        <property type="entry name" value="AT02829P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04750">
        <property type="entry name" value="Far-17a_AIG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="130"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="168"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="212"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP86513.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="27004" checksum="FF40C08242EAFD44" modified="2012-04-18" version="2">MAAHRQRPPTHIPRLATLFHVAGLAVLVNAFRLTSEPDSFFMLMFTKMGGRTHFLTMWGLFFATVTFVLNIMMAIFRKNSRLRDLRKLTLAIGLPLESVITTLYWPIIILNPNMMMLSELELVIPLSVDLALHLYPAVLLWIDYLVFSDRFADSSSRKITLTKDIHLSILQLTLLLTLAYVSRIELLCYFNSDIPLPYPFLNDVDFPVRVAIYSAAAGLCYMYSSIAESLHGLLFRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3KRU2</accession>
    <name>E3KRU2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_12758</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178303">
        <property type="protein sequence ID" value="EFP87017.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331436.2">
        <property type="nucleotide sequence ID" value="XM_003331388.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KRU2"/>
    <dbReference type="GeneID" id="10542094"/>
    <dbReference type="KEGG" id="pgr:PGTG_12758"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12758"/>
    <dbReference type="eggNOG" id="KOG2504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_087682_1_0_1"/>
    <dbReference type="InParanoid" id="E3KRU2"/>
    <dbReference type="OrthoDB" id="6499973at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050327">
        <property type="entry name" value="Proton-linked_MCT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360:SF287">
        <property type="entry name" value="MFS MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11360">
        <property type="entry name" value="MONOCARBOXYLATE TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="44"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="119"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="207"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP87017.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="277" mass="30660" checksum="6768D1E3EF6CD916" modified="2012-04-18" version="2">MWHSAERHQSKSSKLNGTKAGDTLETAVANHGPPVDKGKCAVQYLMAMFTIETIVWGFATSFGVLFNFYQHDPRSPIKPDPHAKLILTLVGTINTGTIAGAAPLFSFWMAKKPGIRRRLMFSGLLTCTLSLFLSAYSTSAFHILLTQGIGYGIGGCALYYSALSHLPEWFDLRQGFANGFVFTGTGLGGLIFPLILNSLLGKYGAKLALQITTVLFAIPIFLAVLFIRPRIPHCRQRQDSLTQSFNQLPSTYQAYIFQPIFIALVVEAWQEVLCWLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>E3KRW7</accession>
    <name>E3KRW7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 10">Proline iminopeptidase</fullName>
            <shortName evidence="8">PIP</shortName>
            <ecNumber evidence="8 10">3.4.11.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Prolyl aminopeptidase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_13261</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8 10">
            <text>Release of N-terminal proline from a peptide.</text>
            <dbReference type="EC" id="3.4.11.5"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8 10">Belongs to the peptidase S33 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.5" evidence="8 10"/>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EFP87042.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331461.2">
        <property type="nucleotide sequence ID" value="XM_003331413.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KRW7"/>
    <dbReference type="STRING" id="418459.E3KRW7"/>
    <dbReference type="ESTHER" id="pucgt-e3krw7">
        <property type="family name" value="Proline_iminopeptidase"/>
    </dbReference>
    <dbReference type="MEROPS" id="S33.001"/>
    <dbReference type="GeneID" id="10542123"/>
    <dbReference type="KEGG" id="pgr:PGTG_13261"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13261"/>
    <dbReference type="eggNOG" id="ENOG502QPPY">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043739_2_2_1"/>
    <dbReference type="InParanoid" id="E3KRW7"/>
    <dbReference type="OrthoDB" id="10249433at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004177">
        <property type="term" value="F:aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002410">
        <property type="entry name" value="Peptidase_S33"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005944">
        <property type="entry name" value="Pro_iminopeptidase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01249">
        <property type="entry name" value="pro_imino_pep_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43722">
        <property type="entry name" value="PROLINE IMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43722:SF1">
        <property type="entry name" value="PROLINE IMINOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00561">
        <property type="entry name" value="Abhydrolase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006431">
        <property type="entry name" value="Pept_S33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00111">
        <property type="entry name" value="ABHYDROLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00793">
        <property type="entry name" value="PROAMNOPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 10" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="5 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8 10" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="12">
        <location>
            <begin position="69"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <feature type="active site" evidence="9">
        <location>
            <position position="302"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR006431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR006431-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP87042.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="351" mass="40079" checksum="38AC62396B94D8EC" modified="2012-04-18" version="2">MTSSKIASSQRAHKLTKTDTEHNSRKRTTDIIMTTSTLFGPVEPFASEHLQVSDLHSLYLEQSGNPEGNPIVFIHGGPGGGCSPEDRRLFDPASYRIIVFDQRGAGKSTPPSCLEENTTWHLVEDIEKIRTHLKIEKWVVFGGSWGSTLSLAYAQAHPERVKGLILRGIFALRRAELEFFYQGPGTSFLFPEYWEEYVSIIPQEERKDMIKAYYKRLTSDDKSVRSEAAKRWSVWECSTSRLMVDQEYIKKAYEDDFSDKFARIECHYFVNGGWMREGQLLEKAEIDKIRHIPAVIIQGRYDCVCPAKTAWELHQAWPEADFKLIPNCGHSAKEDGIAHELVQASNKFKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KRZ2</accession>
    <name>E3KRZ2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13286</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EFP87067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331486.1">
        <property type="nucleotide sequence ID" value="XM_003331438.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KRZ2"/>
    <dbReference type="GeneID" id="10542148"/>
    <dbReference type="KEGG" id="pgr:PGTG_13286"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13286"/>
    <dbReference type="HOGENOM" id="CLU_1993737_0_0_1"/>
    <dbReference type="InParanoid" id="E3KRZ2"/>
    <dbReference type="OrthoDB" id="10433391at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="125" mass="14256" checksum="30AC161754109F33" modified="2011-01-11" version="1">MDIDQSLGESFLDGFRNAGFGIPGDQLTRNPELQEHPTGSKNQLKNNPLNPSDREQENMLLQEHAGPSSSMGKAQVEPADAIKLRTKAIMDKEKFPEEKFKMELANEKWMLHQIRAVHFRVEIKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KT78</accession>
    <name>E3KT78_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13874</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178307">
        <property type="protein sequence ID" value="EFP87503.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331922.2">
        <property type="nucleotide sequence ID" value="XM_003331874.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KT78"/>
    <dbReference type="GeneID" id="10538402"/>
    <dbReference type="KEGG" id="pgr:PGTG_13874"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13874"/>
    <dbReference type="HOGENOM" id="CLU_067168_0_0_1"/>
    <dbReference type="InParanoid" id="E3KT78"/>
    <dbReference type="OrthoDB" id="10297183at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173867" evidence="1">
        <location>
            <begin position="17"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87503.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="241" mass="26469" checksum="4000029C1C67A722" modified="2012-04-18" version="2">MSLLALICLTLKDALAAPTSAVTIAPHPVLFASAPPLAPPIQLTLESSGHLRPRHRKMLMDSRPEEIRNPIPDKMERNHHKTRIPSQEQLSLIKNYISDHMAGSPYVLHPDGSFSAIQKPPGSLKYYYGRVEIFDEEGYSLYETRCCYGFPRSYCACFSFSNCAECCMTSAGCLCGFPIALAICSAGTDLICGSGRLSRALVEACQYEFGRLSTAGYNAWQRIRAARNPAVSPISSEIQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3KTB9</accession>
    <name>E3KTB9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial import inner membrane translocase subunit TIM16</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Mitochondrial import inner membrane translocase subunit tim16</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11 12">Presequence translocated-associated motor subunit PAM16</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_13915</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TIM16/PAM16 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178307">
        <property type="protein sequence ID" value="EFP87544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331963.1">
        <property type="nucleotide sequence ID" value="XM_003331915.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KTB9"/>
    <dbReference type="FunCoup" id="E3KTB9">
        <property type="interactions" value="79"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KTB9"/>
    <dbReference type="GeneID" id="10538443"/>
    <dbReference type="KEGG" id="pgr:PGTG_13915"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13915"/>
    <dbReference type="eggNOG" id="KOG3442">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_101461_0_1_1"/>
    <dbReference type="InParanoid" id="E3KTB9"/>
    <dbReference type="OMA" id="TRTHRMT"/>
    <dbReference type="OrthoDB" id="10262892at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005341">
        <property type="entry name" value="Tim16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12388">
        <property type="entry name" value="MITOCHONDRIA ASSOCIATED GRANULOCYTE MACROPHAGE CSF SIGNALING MOLECULE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12388:SF0">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03656">
        <property type="entry name" value="Pam16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP87544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="142" mass="15368" checksum="DB0FC0ED9FBE587D" modified="2011-01-11" version="1">MSLPRIVSQVVILGSQILGKAFVEAYRQAARNARSGTSHAASAGERGGANLGNMAGDGSVITRKHKMTVDEACQILNVKNVPFSEGPTQTVVSEELANMLKAYERMYQANENTSKYLLSKVVRAKDRISAEVELSQNSKTQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3KTD9</accession>
    <name>E3KTD9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Checkpoint protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_13935</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the HUS1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178307">
        <property type="protein sequence ID" value="EFP87564.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331983.2">
        <property type="nucleotide sequence ID" value="XM_003331935.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KTD9"/>
    <dbReference type="FunCoup" id="E3KTD9">
        <property type="interactions" value="351"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KTD9"/>
    <dbReference type="GeneID" id="10538624"/>
    <dbReference type="KEGG" id="pgr:PGTG_13935"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13935"/>
    <dbReference type="eggNOG" id="KOG3999">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035754_2_0_1"/>
    <dbReference type="InParanoid" id="E3KTD9"/>
    <dbReference type="OrthoDB" id="337750at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030896">
        <property type="term" value="C:checkpoint clamp complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044778">
        <property type="term" value="P:meiotic DNA integrity checkpoint signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033314">
        <property type="term" value="P:mitotic DNA replication checkpoint signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016580">
        <property type="entry name" value="HUS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007150">
        <property type="entry name" value="HUS1/Mec3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12900:SF0">
        <property type="entry name" value="CHECKPOINT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12900">
        <property type="entry name" value="MITOTIC AND DNA DAMAGE CHECKPOINT PROTEIN HUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04005">
        <property type="entry name" value="Hus1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF011312">
        <property type="entry name" value="Cell_cycle_HUS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR011312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP87564.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="299" mass="33064" checksum="3DFD62C21A062DDA" modified="2012-04-18" version="2">MRFRAHLANVATFTKLIDSVNKLARRAVFTFTPEEIRLVSTGDDEDGLRLHGAIKVVDLFGSNYHVESNHHNIIPLEFSTQALASAVRQTSSSPTEIVFRLGRLGRTPYLSLTAKTVGRSGTEYQVEQRVAVRVVKPEEAELLPKPCSPTPDVFLCLPKISDICKVAERLKSLSDLITVSASRNGGFRLGISTEHARVETEWRGLTPFHNRFSQATQDSQGTLRSTSAFFEATVKSRAFLRFLHAHIGDVSLASICEGYCLTLFNYIGDTSQLIETENDERTSGSLFAVLSIVNEDGDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3KU63</accession>
    <name>E3KU63_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein kinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14553</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178309">
        <property type="protein sequence ID" value="EFP87838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332257.1">
        <property type="nucleotide sequence ID" value="XM_003332209.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KU63"/>
    <dbReference type="STRING" id="418459.E3KU63"/>
    <dbReference type="GeneID" id="10534736"/>
    <dbReference type="KEGG" id="pgr:PGTG_14553"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14553"/>
    <dbReference type="HOGENOM" id="CLU_1971611_0_0_1"/>
    <dbReference type="InParanoid" id="E3KU63"/>
    <dbReference type="OrthoDB" id="541276at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:002951">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167">
        <property type="entry name" value="OVARIAN-SPECIFIC SERINE/THREONINE-PROTEIN KINASE LOK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44167:SF24">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE CHK2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="127" mass="14160" checksum="0CFD493285A97A71" modified="2011-01-11" version="1">MPYAPHGTLADQIFQNRHFITNPSHVKPFFQQMVSATSFCHANVIAHCDLKPENILCSSDQVYIADFGLAINQTSSSQFCRGTRAYMSPGQHRTLSIVANSPIIAKSLFVEESQRPKAHELHQGFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>E3KU93</accession>
    <name>E3KU93_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Anaphase-promoting complex subunit 4-like WD40 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_14583</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178309">
        <property type="protein sequence ID" value="EFP87868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332287.1">
        <property type="nucleotide sequence ID" value="XM_003332239.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KU93"/>
    <dbReference type="FunCoup" id="E3KU93">
        <property type="interactions" value="1052"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KU93"/>
    <dbReference type="GeneID" id="10534847"/>
    <dbReference type="KEGG" id="pgr:PGTG_14583"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14583"/>
    <dbReference type="eggNOG" id="KOG0647">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_038526_1_0_1"/>
    <dbReference type="InParanoid" id="E3KU93"/>
    <dbReference type="OMA" id="EAMDQSI"/>
    <dbReference type="OrthoDB" id="256303at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043130">
        <property type="term" value="F:ubiquitin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051664">
        <property type="term" value="P:nuclear pore localization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016973">
        <property type="term" value="P:poly(A)+ mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000973">
        <property type="term" value="P:post-transcriptional tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000728">
        <property type="term" value="P:regulation of mRNA export from nucleus in response to heat stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006405">
        <property type="term" value="P:RNA export from nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032185">
        <property type="term" value="P:septin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000972">
        <property type="term" value="P:transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000776">
        <property type="entry name" value="mRNA export factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024977">
        <property type="entry name" value="Apc4-like_WD40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10971">
        <property type="entry name" value="MRNA EXPORT FACTOR AND BUB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12894">
        <property type="entry name" value="ANAPC4_WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="Anaphase-promoting complex subunit 4-like WD40" evidence="5">
        <location>
            <begin position="46"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="79"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="120"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="3">
        <location>
            <begin position="274"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="14"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="340"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="346"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP87868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="379" mass="41113" checksum="70D621967966DEF8" modified="2011-01-11" version="1">MSFFNSASNNNTGVFGGGSSSTTVTTQDPKDIEVENPPTDGISSIAWSPQADFLAAGSWSNEVRIYEVNGQGQSVPKAAYSHEQPVLEVIWSGDGTKIISGGCDRAARAYDVPTGQSTQVAAHEAPIRKLAWLDINGQGLLATGSWDKTLKYWDLRQPTPAVSVTLPERIYTMDTVFPLMVVGTAARKIHIYHLSNPSVEYKSLDSPLKWQTRCIACFNDAQGYAVGSVEGRVAIQHVEDKQTANNFSFKCHRKDAPTGASRLNANTIPQVWAVNDIKFHKQHGTFATAGSDGTINMWDKDSKTRLKTFDNRGGPITSVSFNRTGTVFAYTVSYDWSQGHSGAQSTGAANPAAGTAASQPNKIMLHALKDDEIKKRPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVK6</accession>
    <name>E3KVK6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14430</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178313">
        <property type="protein sequence ID" value="EFP88346.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332765.2">
        <property type="nucleotide sequence ID" value="XM_003332717.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KVK6"/>
    <dbReference type="GeneID" id="10535818"/>
    <dbReference type="KEGG" id="pgr:PGTG_14430"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14430"/>
    <dbReference type="HOGENOM" id="CLU_2400773_0_0_1"/>
    <dbReference type="InParanoid" id="E3KVK6"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88346.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="93" mass="10198" checksum="D99F43C455C76DF2" modified="2012-04-18" version="2">MEFTPGITTKLQPLSAFEHPSLASSKAGPSSKHQKAEKARYSEIQIKPTRGTPIPSHFYLILNGQTGFIVISTRTVFPIRSIKQSVPSQSSKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVL2</accession>
    <name>E3KVL2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_14436</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <reference evidence="11" key="3">
        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="DS178313">
        <property type="protein sequence ID" value="EFP88352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="DS178313">
        <property type="protein sequence ID" value="EHS64181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332771.1">
        <property type="nucleotide sequence ID" value="XM_003332723.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889197.1">
        <property type="nucleotide sequence ID" value="XM_003889148.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KVL2"/>
    <dbReference type="STRING" id="418459.E3KVL2"/>
    <dbReference type="GeneID" id="10535824"/>
    <dbReference type="KEGG" id="pgr:PGTG_14436"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14436"/>
    <dbReference type="eggNOG" id="KOG0583">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_275214_0_0_1"/>
    <dbReference type="InParanoid" id="E3KVL2"/>
    <dbReference type="OMA" id="REFRHAP"/>
    <dbReference type="OrthoDB" id="541276at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000086">
        <property type="term" value="P:G2/M transition of mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000832">
        <property type="entry name" value="Serine/threonine protein kinase, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001845">
        <property type="entry name" value="Serine/threonine protein kinase, variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343">
        <property type="entry name" value="SERINE/THREONINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24343:SF449">
        <property type="entry name" value="SERINE_THREONINE PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="375"/>
            <end position="776"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="175"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="303"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="523"/>
            <end position="573"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="901"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1060"/>
            <end position="1164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1181"/>
            <end position="1220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="76"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="922"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1062"/>
            <end position="1080"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1090"/>
            <end position="1100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1187"/>
            <end position="1213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP88352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1220" mass="132822" checksum="45F2A459F53AEA6C" modified="2011-01-11" version="1">MSAATIAQIFQPASPAPSSPSAASNPSNPKSKVLALRSLQPSPTPSSVPSPKMSQPSSAHVTFPLSPTLLPPPYSHLPIQHQQQQHQQQQQQHQQQQQQQHNSSIQRPQLARPNSLLSPRPSSPPSPLVLSPLNTTLLPAHAPLSPRPSHPLASPGACPPYLYELAQADRRAGSGFISREPSLPSIMSMPRTRPASPDPIHSRKTSFAARRANLATGVPGSSSSPTSPQVSRRFFTPTSARGLSNLCMFEDGLQHYERVRSPSLRGHSRRGSLIHSVPDKSKDELANEMFELDLSEDFAEETFRQPRWSDDEDDADSERDHEATGARKLFKVGDRVGVGTLHQGYRVRDLFDTSSRSGCRLSKASTESSGESGILEVVRQIGVGSYAVVYLVREVLYDPALEPEPTALDSTLSQTPKASDGFASLSKLDPLASSRTKGSSEGVVYGRDFALKCLCKRNLSDELLVLQRGEAELHRSLPLHNNIVALHRALETPNWLFLVIEYCPGQDLFYWLEQARDSQDLESLSSRTNSCAPHLPSSIQPHLHSTEDKSREVGSAQVTAPPLNTPPSPSLLASTADDEMLSRRRLRLISKMFVQMCEAVEACHQAGVCHRDIKPENFIVVDDRKGTLGDNWKAQSTEHQIGVRSSVTVKITDWGLGTSSKACEDFDCGSKPYMAYECRNNLNPTYEPIQADVWSLGIVLLNLLYHRCPWADPSCSDPDFREFRHDPVSFLQGRFDGMTQSVATFLAENVFVEVGAGRSQRRISAGKFGEWAQNLLHHMGGGQSHASVSDATIAMSPSYTRGVIDQRRSLRGSSLGGLSKRSSQLFHPINLPPAVPEVLSPPELSPTELPVVIEKEVCQIEKQPLNPWPEKSSDLKDFADEPTEILTDMVLNPGAVKESADVIGEDSESCTSASKVGSDNPKRRKRGARKGRKPEGRRNGSEAGYLSSTNEQEGEVDILDGLAEASQSLAREISQATKTMKPSDEGATSLTTTKRKLNIAERMMEKFRDGSNPDLQAFVARARAREAAYMGKATSNTASAPAQLQDVNRHGQRNVFSRAAMSTTSSSGIATTMSTSSTTSWGGGSDCSSFSKPPTHWTSASHRRERIQKTTPNANVTAPIPSTPLLPTAPISRKKAPVNHPSGQTETTTTITSNPPVNNKKEESKNKLAQLLTTFKRFNSTVGNHHSSSSSSAAVPAAATTTTSTTRHPSSTSNFITDSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVY4</accession>
    <name>E3KVY4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14037</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178314">
        <property type="protein sequence ID" value="EFP88459.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332878.2">
        <property type="nucleotide sequence ID" value="XM_003332830.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KVY4"/>
    <dbReference type="GeneID" id="10540762"/>
    <dbReference type="KEGG" id="pgr:PGTG_14037"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14037"/>
    <dbReference type="HOGENOM" id="CLU_2759021_0_0_1"/>
    <dbReference type="InParanoid" id="E3KVY4"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88459.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="70" mass="7617" checksum="C6353AF5279DF98F" modified="2012-04-18" version="2">MTESATRKGRECGELLHGVYKRWPAAGCHPAFLAPVGIKVRIIQRSWNPYSNQEGLQEKGAHKGGPGNCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3KXG4</accession>
    <name>E3KXG4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_15125</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178318">
        <property type="protein sequence ID" value="EFP88922.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333341.2">
        <property type="nucleotide sequence ID" value="XM_003333293.2"/>
    </dbReference>
    <dbReference type="GeneID" id="10542201"/>
    <dbReference type="KEGG" id="pgr:PGTG_15125"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15125"/>
    <dbReference type="HOGENOM" id="CLU_003292_4_0_1"/>
    <dbReference type="InParanoid" id="E3KXG4"/>
    <dbReference type="OrthoDB" id="3254696at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052055">
        <property type="entry name" value="Hepadnavirus_pol/RT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050:SF7">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47823">
        <property type="entry name" value="lambda integrase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="146"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="368"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="17"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="146"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="386"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="405"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="449"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="469"/>
            <end position="484"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP88922.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1387" mass="156745" checksum="DE31AF8E22BA1DC7" modified="2012-04-18" version="2">MSNPNSIQYLSDRVKDSSQQANQSPLPGQQTSQIPLPNSSTPKSVAHPLNKENRPTGSLNPAPKGKETTSTKAGEPDPALQSSVTKATLKNPIAEIVDEGNETLPQSRSTNARAKEILLAKAVKAQEDGDDAKADRFFAMFDTLSKESPQPANEKTTPPTTQTISGPSPTNRKRPIESEGTTQVRGIKFIWANTNSHDDGGFTPYFHKNLLELKGPIPLTIFNKTWQEEALSHHSKNRPKSEESSAEKNLRYHGLAVPDEWLQSFSDWTLNHQCFYETIRDRYNYPVLAEWILLHKANCDRLQKKHGFMVALRYDIRIRNNAFAFRVEKDGEESFSNISLFKPETADDAHSESRNFNELGVRDNPYALGGPRYGWDPHTGQKRTKTATSATPNNNSNNGKAPLAGTSQSLPQTPTNSLPPKPDQNRPPRASGYKGRNYNPNHGSATRRRGNEEVRAHTEPQSLPLVFSDPHHAEPAKEGTPDWPDAIRCEMNVREWERALEKAGLAQEFSDVIRGFKEGFDQGIPNHNLGPATPYFTPPNHQSALLAQDKIEQSMKKEVEAGRMFGPYTHEQLMRKFSFFRTNPLGAAVNGDGSIRPINDLSFPRNDPLTPSVNSFVDKLDYATTWDDFENVSKFFRRQTGPLLLALFDWEKAYRQIPTAKSQWAYLMVRDFNGGILIDTRIAFGGVAGCGSFGRPADAWKQLMLHEFDLVTVFRWVDDNLFVKHPDSNVEMDHIVARSESLGVKTNSTKYSPFKEEQKYIGFIWNATRKSVRLPDDKKYQRVQQVKEFLQIGSEFSFKQVEVMAGRLNHVSYLLPQLRCYLNSLYRWMNAWVYRSKDLPLPPSARVDLQEWLTTLLSFKETRMIRDPDPIEIGWMGDASTSYGIGITIGRRWAQFQLTKDWDRGPEPKRDIAWLETVAIRLGLIALAQLSVKRGKTIIVWTDNTTTESAILKRKSKHQAVNDEWKIIQRLLVEMELDIVSRRVSSGDNVADALSRGDRRGRDPQLQIPITVPLDLEHRMFQSRDTPMMLDLKRVSAITSNGSEPREPTAQERHYLLGYRWNTLLSYNAAIKKYQNFARDTGKPTFRLPLTAQDIYDFCCWAGRTAESSTTHEISANTLSKYLAGLQMWHVYHNAVYPTDSKTKVATFLKSSAYIDAETEKKPKKKPVTIKNLVKLTELLIDGDPFQKALLDLSVVAFWGMARLAELTYNEGSGPLRRTASLLTTDVELVYKERENLAKLTIRGAKTADPGQSQIIYLKELPHMLCPVLAVKRRLAEADGAETSLFGYTDHQGERVHLTKPQTTRALSKIWGACGFEGVSGHSFRVGGASIQVALGVPISEIRIRGRWASDAYKLYLRDFTKEEMSKTNDLLARLEECWMHVPNPLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3KYQ8</accession>
    <name>E3KYQ8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Target of rapamycin complex 2 subunit bit61</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15296</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP89454.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KYZ5</accession>
    <name>E3KYZ5_PUCGT</name>
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        <recommendedName>
            <fullName evidence="1">DUF7872 domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="2" type="ORF">PGTG_15669</name>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
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    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178323">
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="entry name" value="DUF7872"/>
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        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN"/>
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    </feature>
    <evidence type="ECO:0000259" key="1">
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            <dbReference type="Pfam" id="PF25278"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP89520.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="487" mass="54381" checksum="6900D77153FAB624" modified="2012-04-18" version="2">MLSWFTDHLLKTKSSFSFVILVLLIANQLHAIHSATIPYGRGRLLDNLSNSTNLQSFYNVSSASTRPSHRYLSSHHTSTGIPQKDTDSVLLDECMSRPLSPKLWMEMGMNEFLRDYRNGENLNLLQLANQVNLTNFDCGIEKLCYADQLCQPVRGKEWYILVAAQEWNTFMNAAYQSIGWAMTMMQGVAPSLVHDFYPDLPDTWAIIKAILTFASALAKVIPTEGLLTYPKWCYQLVQGEFGLYAGVSNLMDDIIMKNPVNQHDKWTAFSYALSKSQDRAQMAIANLSQSIIEAGISTENGIYGVLKDGNFLMGRSKTHKKTHEKLDNIAGDKESELKVAVQLHLLAAIWEQQNYFITRGSDPCTGSGPNGAWGGDKVLSYCGPDNVMMNIIQAKGKHAINKVHNAKLVTVKYNFSVKSLTEAAWNCQKQSGKFEFDVWNSTDPVKFDDQCSFNLPVCDLTRPDIREYRDSGAGTVAACRIIGGLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3KYZ8</accession>
    <name>E3KYZ8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cytosolic endo-beta-N-acetylglucosaminidase TIM barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_15672</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178323">
        <property type="protein sequence ID" value="EFP89523.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333942.2">
        <property type="nucleotide sequence ID" value="XM_003333894.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KYZ8"/>
    <dbReference type="STRING" id="418459.E3KYZ8"/>
    <dbReference type="GeneID" id="10547491"/>
    <dbReference type="KEGG" id="pgr:PGTG_15672"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15672"/>
    <dbReference type="eggNOG" id="KOG2331">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016433_0_0_1"/>
    <dbReference type="InParanoid" id="E3KYZ8"/>
    <dbReference type="OrthoDB" id="284473at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033925">
        <property type="term" value="F:mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006491">
        <property type="term" value="P:N-glycan processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032979">
        <property type="entry name" value="ENGase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005201">
        <property type="entry name" value="TIM_ENGase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13246:SF1">
        <property type="entry name" value="CYTOSOLIC ENDO-BETA-N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13246">
        <property type="entry name" value="ENDO BETA N-ACETYLGLUCOSAMINIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03644">
        <property type="entry name" value="Glyco_hydro_85"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cytosolic endo-beta-N-acetylglucosaminidase TIM barrel" evidence="2">
        <location>
            <begin position="81"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="35"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="612"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="612"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03644"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP89523.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="863" mass="97474" checksum="CAFE5AD4245C964B" modified="2012-04-18" version="2">MPISSSIQTTINNSRKNESYFDSLNELVRYFQSRPTPGLVPSPTHSSLPDQQEDQSRPETQKHPQLVVTHDFKGGYYENPFKRTYSFEWMSSVDIFVYFSHHRVSIPPKQWITAAKTHHTRILGTLIFENDSFEDLRFLLSGPQLDGSSQTNPPTKYYTSLKNTPISTYFADRLIDLAIHHQFHGWLINIEIDVLKSLRNHALAGLFVNAIKIWVEYLRKQGQIRVGSDWEVSWYDSVSYHDGSLRWQSALRPQHNLTFFSAASSIFLDYHWSARHLRDTQLLVDQVFDLHRPPDSYPRETLETLDLSFQHSMDVKVATHQLRRSVLYGVDVFGRGCPYGGGFSSWKAALDILNASFSVALFAPGWTWESDVLQQDRTNSEESDPQWWDLWWRDERYFWIGLLEDPDPSSNNDQKGSNEKLVSLEPPNAYIDSLRIARETKQEEPKKGVGFSELPQHKPLLELFPVRHKRLSTSFYTNWSLGSGHGIWVSGEHHFDPLHGIGDWTDLAMSFPKHDLSIRGGSVWSVCDGTIVQTGVARCELVEDVGWSGSGCVEIKEQVNDQAPRGTAKYLWMNTTSVRCSSEMECRLVWKPIDAQGASTTPLGVVFQVNDPSTQKTNSRNDPTISGNFSDASARPPVVTLSLVPRSNSALEASNMETRELGNGWYESTCQLKNSSSSPSTIVERLGITTTPGSRFSHYVGEINLTPSSHSDRVIHQTQTQPPNSLEVIWQPDSTQQPITIDCPAANSQSRFINKPGKILWKFPTSSGSMETNDNHHEEVLAWLIFIANDGSSDCNLNKDEQMNHRLIGVVKGFQEFDINGFNAPLIVNDRPSNGKGVEIWNVIIKGIGMHGRVVCIGFCSLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZ36</accession>
    <name>E3KZ36_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_15710</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178323">
        <property type="protein sequence ID" value="EFP89561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333980.1">
        <property type="nucleotide sequence ID" value="XM_003333932.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZ36"/>
    <dbReference type="GeneID" id="10547530"/>
    <dbReference type="KEGG" id="pgr:PGTG_15710"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15710"/>
    <dbReference type="HOGENOM" id="CLU_042830_0_0_1"/>
    <dbReference type="InParanoid" id="E3KZ36"/>
    <dbReference type="OrthoDB" id="10280720at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP89561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="386" mass="44417" checksum="540536FEB5CF0F59" modified="2011-01-11" version="1">MSARQTAPDVDAELIKLIRTCYRFTPESNSQEEEQVSPEEDLKTRLEKLNRVESTILPTIRDLVTELLEALDLPAKDSISPQPKLDEASEIVPRLTSLVEDLACSVLPPDYSNPEICRLIDQNYGQLNQFRSCYFKKKITEFIKEDLRRLFSSLSSFVSNGEYYPPDYEPSDRDKPHQEGRAGRIQIIIPRIINIIRGSNFDIIQSIWNECEDLLASDINDIHWCINLTNTRAPARIVPDFSPLIPAVLEILDQTLVLLKMCRLFSRKIGSTRLFKFAEGISPADLASFGDVSEKFQIHVQSLIVYMETVAEFQEKPPGWNFKHEAEVISHDVHFCLDFIRSHMVPSYPPQTVDEVMDRLFGTFTQQFFPTLDRYIARLTQFQADH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZB5</accession>
    <name>E3KZB5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Serine/threonine-protein phosphatase 2A activator</fullName>
            <ecNumber evidence="8">5.2.1.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Phosphotyrosyl phosphatase activator</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_15603</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for PP2A-like phosphatases modulating their activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a direct target of the PPIase. Can reactivate inactive phosphatase PP2A-phosphatase methylesterase complexes (PP2Ai) in presence of ATP and Mg(2+) by dissociating the inactive form from the complex.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the PTPA-type PPIase family.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="8"/>
    <dbReference type="EMBL" id="DS178324">
        <property type="protein sequence ID" value="EFP89640.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334059.2">
        <property type="nucleotide sequence ID" value="XM_003334011.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZB5"/>
    <dbReference type="FunCoup" id="E3KZB5">
        <property type="interactions" value="65"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KZB5"/>
    <dbReference type="GeneID" id="10547694"/>
    <dbReference type="KEGG" id="pgr:PGTG_15603"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15603"/>
    <dbReference type="eggNOG" id="KOG2867">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030733_0_0_1"/>
    <dbReference type="InParanoid" id="E3KZB5"/>
    <dbReference type="OrthoDB" id="16120at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000159">
        <property type="term" value="C:protein phosphatase type 2A complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008160">
        <property type="term" value="F:protein tyrosine phosphatase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04087">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1150:FF:000002">
        <property type="entry name" value="Serine/threonine-protein phosphatase 2A activator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004327">
        <property type="entry name" value="Phstyr_phstse_ac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043170">
        <property type="entry name" value="PTPA_C_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037218">
        <property type="entry name" value="PTPA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN PHOSPHATASE 2A REGULATORY SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10012:SF5">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN PHOSPHATASE 2A ACTIVATOR 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03095">
        <property type="entry name" value="PTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016325">
        <property type="entry name" value="Phstyr_phstse_ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140984">
        <property type="entry name" value="PTPA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0697">Rotamase</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="18"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="25"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011019"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP89640.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="409" mass="46234" checksum="E64ECFC4C76B6D08" modified="2012-04-18" version="2">MVCNRFGSEVEYISDQDIRHKPKKNQLQTHKTMDTNNEPANPPTKEPGTTPKSITKPRKLIISNGHLQAFIDSVTHAEVVDFIESLNQSIIGLPLDHPLPLSDNIDRLLEILENIKNIALAHPPVDNKASRFGNPAFREFYDQLDQGSEALHSSLAIPEDKRVELSTYLTESWGNRSRIDYGSGMELNFLCWLLCLKKLKVFGSEDYPAVVLKVFWKYIEVMRYLQSTYWLEPAGSHGVWGLDDYHFLPFLFGAGQLRNHKYLKPKSIHDPDVLEGFSHQYMYLACIQFINSIKTASLRWHSPMLDDISGVKTWDKVNSGMIKMYKAEVLAKLPVAQHFLFGTLLPFPDGHIQEVESKTDGSLVVDHAGHLHVKGQQGWGECCGIPIPSSFAAATAENKNPAIRRIPFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3L0F9</accession>
    <name>E3L0F9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_15905</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178328">
        <property type="protein sequence ID" value="EFP90057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334476.1">
        <property type="nucleotide sequence ID" value="XM_003334428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L0F9"/>
    <dbReference type="GeneID" id="10546035"/>
    <dbReference type="KEGG" id="pgr:PGTG_15905"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15905"/>
    <dbReference type="HOGENOM" id="CLU_049647_0_0_1"/>
    <dbReference type="InParanoid" id="E3L0F9"/>
    <dbReference type="OMA" id="ETREQMC"/>
    <dbReference type="OrthoDB" id="2507399at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34409:SF1">
        <property type="entry name" value="MYB-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34409">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="131"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="170"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="417"/>
            <end position="447"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="275"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="15"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="131"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="175"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="432"/>
            <end position="447"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP90057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="447" mass="48314" checksum="6AAA22B63FFC037F" modified="2011-01-11" version="1">MPAEVNLPSRPAPCPQTQVNQQTTQPLTLDQPPSTTQHTTTNATGTANQKRSGRQPGSQGYSGADCTALVAIVKDVLPLGSNKWDNVHFKYSQYAMANGQTVRDVEPLKTKEAKRVNLLIKDRAKNLAFVDEDGDEEVESDDERNGVGNPIPLSPDDTLLLGWDATHPELPVDPANTSVEESDSVAPSGTGMATQAPSTNVGDLGARDVANSSHRDCNTLVHCTQSCLSGSTGTLPIDSSNCRSRSSQASGLHDSLAAYFNPEACEARERESSMTQFYEVRLQEATATINRLQDEATKLRKGINMQVLRLQDDLEKARSEVAQKNSENQELCHRLEMMQLWMEFQSHSFFQPGMISSNMAQGLMNPNLNATNQAQPNVAPAFLHPSGIPVQGNITASYIQPNGNHTIMRGFAPTSALGNPPPTTFGTNTTGAQQWDSTGVAPNQSTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3L0R1</accession>
    <name>E3L0R1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_15963</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178329">
        <property type="protein sequence ID" value="EFP90115.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334534.2">
        <property type="nucleotide sequence ID" value="XM_003334486.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L0R1"/>
    <dbReference type="GeneID" id="10546093"/>
    <dbReference type="KEGG" id="pgr:PGTG_15963"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15963"/>
    <dbReference type="HOGENOM" id="CLU_1653012_0_0_1"/>
    <dbReference type="InParanoid" id="E3L0R1"/>
    <dbReference type="OrthoDB" id="2508781at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172371" evidence="2">
        <location>
            <begin position="23"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP90115.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="160" mass="17999" checksum="A3AE125910350123" modified="2012-04-18" version="2">MRFLQLLFIACAMILPNSIILAMEEIAEVGEQNLGQRTGRLFNHRGSTSSDVKSEGLADAPENLTSNMEPESHGLDPQENVNGNQLLHKVQSLYWYWAPELLMLALAGPLMKHWPELFEPRQSTYNLIPGLWIAAAPGMAWNFFRGSKFISKFISKASHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>E3L2V0</accession>
    <name>E3L2V0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="4">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PGTG_17074</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="4"/>
    <dbReference type="EMBL" id="DS178338">
        <property type="protein sequence ID" value="EFP90875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335294.1">
        <property type="nucleotide sequence ID" value="XM_003335246.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L2V0"/>
    <dbReference type="STRING" id="418459.E3L2V0"/>
    <dbReference type="GeneID" id="10530782"/>
    <dbReference type="KEGG" id="pgr:PGTG_17074"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17074"/>
    <dbReference type="eggNOG" id="KOG0201">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_63_23_1"/>
    <dbReference type="InParanoid" id="E3L2V0"/>
    <dbReference type="OMA" id="ENMPGWK"/>
    <dbReference type="OrthoDB" id="248923at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06609">
        <property type="entry name" value="STKc_MST3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000411">
        <property type="entry name" value="Probable Ste20-like kinase Don3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000092">
        <property type="entry name" value="Serine/threonine-protein kinase 24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046409">
        <property type="entry name" value="PDC10_dimerisation_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050629">
        <property type="entry name" value="STE20/SPS1-PAK"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012:SF10">
        <property type="entry name" value="FI20177P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48012">
        <property type="entry name" value="STERILE20-LIKE KINASE, ISOFORM B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 20" id="KW-0418">Kinase</keyword>
    <keyword evidence="13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="19">
        <location>
            <begin position="28"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="306"/>
            <end position="600"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="54"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="9"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="313"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="18">
        <location>
            <begin position="357"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="380"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="18">
        <location>
            <begin position="404"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="467"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="18">
        <location>
            <begin position="507"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="523"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="575"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="18">
        <location>
            <begin position="587"/>
            <end position="596"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="EFP90875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="665" mass="73587" checksum="B5A183FDE9C9F51C" modified="2011-01-11" version="1">MSHLPPPRNSTSTINQSNERQADPEEFYVRQDRIGKGSFGEVFKAFDKRTRKPVAIKVIDLESAEDEIEDIQQEIAILSQLDSSYVTRYHGSYLKGTSLWIIMEYCSGGSCSDLMKPGVFREEYIAIILKELLKGLDYLHQEGKLHRDIKAANVLLSSTGEVKLADFGVSGQLTATMTKKNTFVGTPYWMSPEVIKQSGYDSKADIWSLGITAIELAKGEPPYADLHPMKVLFLIPKNPPPVLDGPEFSKTFKDFISECLKRDPNARPSAKELLKHKFIRNSKKPSYLTELIERLERWKIEGGHQNKIAGQNNDHEDGDQAHDDHSTPNDMWDFGTIKKGTTRRLPPGPSQNPGAASSSKPNPQRSQQPPPPAGVIQRDYGAEPKFETVRHTLPPTSPPDSRPSKPKKSPGQKKKKAPASEPSGDKPQPRRKSSQAHSGVPGSEGLGVQVESAHHRVYNGSGGQHSQDIRSSREFPRSQPAPLSANNIAAQIGNGKGSIRAKHPPASSDTHQGSTSKRPPNGRQKEPAHRADQYRDPSLAERRDPQPSSSKNHKKTHPEHDRDTQQAHHHHHHHQDSAEDESPHAEGEEEDEDQEGVGEILDTVVIPVIQSIRERIKGNAAAVAAIDKFQLAIETAEREVPGLLNVFVSEVVDSVEQVDNDVVDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3L2Z8</accession>
    <name>E3L2Z8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF4219 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_17195</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178339">
        <property type="protein sequence ID" value="EFP90923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335342.1">
        <property type="nucleotide sequence ID" value="XM_003335294.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L2Z8"/>
    <dbReference type="STRING" id="418459.E3L2Z8"/>
    <dbReference type="GeneID" id="10528337"/>
    <dbReference type="KEGG" id="pgr:PGTG_17195"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17195"/>
    <dbReference type="HOGENOM" id="CLU_051119_0_0_1"/>
    <dbReference type="InParanoid" id="E3L2Z8"/>
    <dbReference type="OMA" id="QAYHEIV"/>
    <dbReference type="OrthoDB" id="2505440at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027532">
        <property type="entry name" value="Mdm12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204:SF1">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="247"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="253"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP90923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="316" mass="35603" checksum="2D8DB59378656234" modified="2011-01-11" version="1">MSNSRNPPLLLNDSNYSTWSFLMVAKLSKIDALDVVLGTIKKPKLEDPEKPTKEALAYEEINRIAYIEIIEHLDNNHLAYISQILVDETSFCGFSVWQILKKKYAGDDYVAKDLALEKFLDLDYHGSITDFIFEVRAANQKLISSKIGLDDQVKNAIILRKLPSDFQSLRTVLSIGCSSDTVTMMLNRLERHAAQNHLDRSSSMPSQQALLTTDDKFYMCPHCKKGFTICSHCDKAGHREAICFEKHPDQRPSKTSNPSAPSKSKPVAQAHLAISSPTSHAVHGFTAEQIESERNFQAMLANPEFKKLNINAEYRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3H4</accession>
    <name>E3L3H4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_16961</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178341">
        <property type="protein sequence ID" value="EFP91099.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335518.2">
        <property type="nucleotide sequence ID" value="XM_003335470.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L3H4"/>
    <dbReference type="GeneID" id="10528678"/>
    <dbReference type="KEGG" id="pgr:PGTG_16961"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16961"/>
    <dbReference type="HOGENOM" id="CLU_148184_0_0_1"/>
    <dbReference type="InParanoid" id="E3L3H4"/>
    <dbReference type="OrthoDB" id="10346042at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP91099.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="107" mass="11780" checksum="A4D2D406C0193715" modified="2012-04-18" version="2">MSAAACNTYPIENFCIYLMPKQPAPVYAPCQSDLLPSVFEDDDDDIIEVDFTVLQKAKNVMAKLFTRGDPAKPSSPKSFSPARLLAKTLPSKFSRFRTNSLNSGSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3P1</accession>
    <name>E3L3P1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sphingosine N-acyltransferase lac1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16911</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178342">
        <property type="protein sequence ID" value="EFP91166.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335585.2">
        <property type="nucleotide sequence ID" value="XM_003335537.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L3P1"/>
    <dbReference type="GeneID" id="10528828"/>
    <dbReference type="KEGG" id="pgr:PGTG_16911"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16911"/>
    <dbReference type="HOGENOM" id="CLU_1866100_0_0_1"/>
    <dbReference type="InParanoid" id="E3L3P1"/>
    <dbReference type="OrthoDB" id="2508419at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174358" description="Sphingosine N-acyltransferase lac1" evidence="1">
        <location>
            <begin position="24"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91166.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="137" mass="15456" checksum="4900EBB02CC52446" modified="2012-04-18" version="2">MKTPNTILMTTILSGFIYFTAHGAPTTFGFKKVDIPQWAWYQSLLDEQFLDDDMARFGGPISHHAAPASHTNFPTWGLVFANGNQPNKVYITNQGRGPLQYLLRRTGTQHYLDHTIQPHHQHVVHGPAVTILCIKHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3L4I4</accession>
    <name>E3L4I4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17709</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178347">
        <property type="protein sequence ID" value="EFP91459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335878.1">
        <property type="nucleotide sequence ID" value="XM_003335830.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L4I4"/>
    <dbReference type="GeneID" id="10529460"/>
    <dbReference type="KEGG" id="pgr:PGTG_17709"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17709"/>
    <dbReference type="HOGENOM" id="CLU_1098941_0_0_1"/>
    <dbReference type="InParanoid" id="E3L4I4"/>
    <dbReference type="OrthoDB" id="2503229at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501:SF5">
        <property type="entry name" value="HAT C-TERMINAL DIMERISATION DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47501">
        <property type="entry name" value="TRANSPOSASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="258" mass="29186" checksum="43F76E4079F1D427" modified="2011-01-11" version="1">MSRKSYGLGISAQPSVPGNELRRSSRKKKTIQQNDSDAGSIIDLREDSEADNSKIPAAKPRPEKYDSPLEFFGEPFYPAKTTEEECKKKPITYNCKWCKKQVRGGHNSDANLRKHRDGSNQTGRDGSGCPNRSLAIQAGAKIPPTVNETRAQAEKKDNGGPKLTAFFGRTEKFDKKILNQILMIWQTRNALPWSRIEDFDLQAAFHYCQSDAILFKRKWVASESKQLYVSLQGTMLQMLKVTQDLREMTRFHGHGPAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3L5T4</accession>
    <name>E3L5T4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17936</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178354">
        <property type="protein sequence ID" value="EFP91909.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336328.2">
        <property type="nucleotide sequence ID" value="XM_003336280.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L5T4"/>
    <dbReference type="GeneID" id="10531858"/>
    <dbReference type="KEGG" id="pgr:PGTG_17936"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17936"/>
    <dbReference type="HOGENOM" id="CLU_863666_0_0_1"/>
    <dbReference type="InParanoid" id="E3L5T4"/>
    <dbReference type="OrthoDB" id="2507256at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="268"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="284"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91909.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="322" mass="35356" checksum="35BDA9EE5B9C1A74" modified="2012-04-18" version="2">MKAHRTEKEKLKTPREELKADRKRLAQEEKNQARKSTGTGSKSSKNEDCGKNFDHKDFENICSYLKTPGHYTDLFGDGQKTSVGQAKLTKAKAFDVFAVWMNSLNPDLQLSGRRLQQRLTSYKQKYIKSKNFEEKTGAGVLDEHGPQTLAEILEDMCPCHDRIDAIFRDKPNVTPMHEFDHSLASATLLPNEELTDGESSGSSSSHGLIGGLNYNPILEDLDVEMLSPTSAVSTTHAVINDSCEYQQLLRYGRLPAVSNNPVVPAIQSDSPAIQSDPIPENDPISDSATTHHNQPAVPIPSGLHSNFGGPYQCSFGSATSKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3L804</accession>
    <name>E3L804_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_18194</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178370">
        <property type="protein sequence ID" value="EFP92679.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337098.2">
        <property type="nucleotide sequence ID" value="XM_003337050.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L804"/>
    <dbReference type="GeneID" id="10539009"/>
    <dbReference type="KEGG" id="pgr:PGTG_18194"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18194"/>
    <dbReference type="HOGENOM" id="CLU_660792_0_0_1"/>
    <dbReference type="InParanoid" id="E3L804"/>
    <dbReference type="OrthoDB" id="2495769at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09917">
        <property type="entry name" value="F-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP92679.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="416" mass="47498" checksum="EC5E761FEEEE8998" modified="2012-04-18" version="2">MDGALRSPTSEHIRWMKASPTNLSLLPVELVEHIADHLQEQLWPPVDSVITDWNSYPSLRSQRDLCALSLVCRALNKIIAPRILKNVVHDFKSKSSCETRFDPYRYKWLSPRSFPSQLAPKWIRVLYLHDLFRDQDPAIETKNMAELLQNCSGVEVLVATGFCASVLLHTLRTASLKEMDSLKVLKIFSYAINAKSILAAFRTFPNLTSLTIDVVNIEGTGTIPPHSLPSRASLITQLDICARLYAPEEYESLCAVLTHIVPTLKVVHIHGEYSEELHRILSLLRRSPVESLLLNFKNKFPESLLSISLPTLKTLSISTYGYTSPQLWESNLARSVRKLIIRSSGYPIDINHLHRTVDIPNLKVLQLQSLCKIRDQEEERRWDARVREWCSRKGVSFVNKNSDSNILTPQDDYRHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8B5</accession>
    <name>E3L8B5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_18569</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178373">
        <property type="protein sequence ID" value="EFP92790.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337209.2">
        <property type="nucleotide sequence ID" value="XM_003337161.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L8B5"/>
    <dbReference type="STRING" id="418459.E3L8B5"/>
    <dbReference type="GeneID" id="10542503"/>
    <dbReference type="KEGG" id="pgr:PGTG_18569"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18569"/>
    <dbReference type="HOGENOM" id="CLU_073655_0_0_1"/>
    <dbReference type="InParanoid" id="E3L8B5"/>
    <dbReference type="OrthoDB" id="160054at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35396">
        <property type="entry name" value="SMALL SECRETED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35396:SF1">
        <property type="entry name" value="SMALL SECRETED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172517" evidence="2">
        <location>
            <begin position="25"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP92790.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="258" mass="29179" checksum="A466917E94D39065" modified="2012-04-18" version="2">MAIYSQMLLIFWIMWLFMSPFALANWDEATGHIRDYKPSNTWLSRNRRESLILTFRSKPEVLCSDLPPIVDFEKPNCAVGTFKYRNVQEIQDFTFLMVVQLFATFTVQHADDNYHGCPYGICCAYTVVPSPRDFVADFTNGHAFFWHNLGGMAGLGTNPIRDPQTGQPGYETSDGVFHVGPPNYKLRQNGHDSHYPGFRLPPAWPSLKYPDWMIKQPAHPKCGTPKGPNLDPGQQKGGQYKPAPATAYSPPRSPRLPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8D6</accession>
    <name>E3L8D6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_18590</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178373">
        <property type="protein sequence ID" value="EFP92811.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337230.2">
        <property type="nucleotide sequence ID" value="XM_003337182.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L8D6"/>
    <dbReference type="STRING" id="418459.E3L8D6"/>
    <dbReference type="GeneID" id="10542524"/>
    <dbReference type="KEGG" id="pgr:PGTG_18590"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18590"/>
    <dbReference type="HOGENOM" id="CLU_011407_4_0_1"/>
    <dbReference type="InParanoid" id="E3L8D6"/>
    <dbReference type="OrthoDB" id="2505730at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041320">
        <property type="entry name" value="CxC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040521">
        <property type="entry name" value="KDZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18802">
        <property type="entry name" value="CxC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18758">
        <property type="entry name" value="KDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxC1-like cysteine cluster associated with KDZ transposases" evidence="2">
        <location>
            <begin position="139"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP92811.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="962" mass="110858" checksum="F96B922BDC0213F8" modified="2012-04-18" version="2">MAVKVYKDRVSGTLAQRLRRIQNAERIAATRARFLGQQSARRQREEQQQHQDQQTANDHMMDNPYEPNYGDNLGSNEHPSADLLDDDWITLDETNDDDLDRAIEADKERWRQEAQQFNWTSIIEQLHAVYMDLKDRTNNWAGQNAYSTFLNCRCLPHTMNSRLVDMVDITRQHRINFRFCPCTSDAVRLLQHGFLPASPIRPQTAFSLPLLIFHNHLWNNCHVGALPFTTALTEWLEPRSGRLFARKQKHAREMRKPFSAAVDLYRSLENKSTELVFKVLQFEPHDILATESCPACFGPRPPNAQDYPDSTRDKLIVCLDGNFQHRHHANASHDYRPLQTPRIFLKADEFEDMKLAIRNKEIELSPPTKADRCADSHKAADDKRNEATWKGCDDTGLMGCCCRHDSAIYMGNIFKSGEQRCLPIAIIKRLLSECGEERNIGILYDIGCSLDKYMSGRNLLQEHRHRIKFATSVFHAYVHNWVCQLDYHPRLNIGWGLSDGEGLERLWSYLSPLVSPLRYATRNHRLGSIAHRLKHHNTKGIRNLPYWLQRKYQAALKRRSEARSVISGLLEMPNPHSTTTTNYTQEFFIQQWKAQHEFQANHTEAEEERRIKKRLRGPEIFLATEEEVNDLLKDIAEHSDKLRKELDELTGEHNMGGVESDDESKLLLLLWSAKADLFVQAVQIRAEKRPIDDSKTIGTHLGTKLKEKIYKALNERRPAVKKYIDAFNRCYANYVAKFPNQKLSDAADYPLTYDDFITFDMDHRFWNDGLYYHCKAPWAVDCDVRAGITATLSLSRVQEEFQLLAQELCQSIGWGVSYYNRLSNSVAYLAGRISQLDLRQETGAGELAPDHVDQLTLGNFSPKHKYIVLKRDIRQRLVAHGAMMEEWSDKIIWLWERCQPLSNRGYISDWEKMIVNIQAGNPIGAETSAGVEEEMEETVLAVELDDGEDATDGDLMSATETG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3LA20</accession>
    <name>E3LA20_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19413</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178391">
        <property type="protein sequence ID" value="EFP93395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337814.1">
        <property type="nucleotide sequence ID" value="XM_003337766.1"/>
    </dbReference>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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</entry>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP93506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
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    <protein>
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            <fullName evidence="2">Uncharacterized protein</fullName>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
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                <person name="Schein J.E."/>
                <person name="Zhong S."/>
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                <person name="Grigoriev I.V."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <dbReference type="InParanoid" id="E3LBK2"/>
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            <begin position="1"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP93927.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
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    </evidence>
    <sequence length="111" mass="12458" checksum="FBE2B07A49889B74" modified="2012-04-18" version="2">MERKSGKPRRQLPSRRKELSSADDIAPFSIALSSLDIPLTPAGEARRSYTTSTSIFPHFRSPPLHSSVRFNARFDLLFASTLLIIEQRATGDDLNAHVYRPCQLIVSNLAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3NXG3</accession>
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        <recommendedName>
            <fullName evidence="3">DUF7872 domain-containing protein</fullName>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS989936">
        <property type="protein sequence ID" value="EFP94262.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="XP_003338681.2">
        <property type="nucleotide sequence ID" value="XM_003338633.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NXG3"/>
    <dbReference type="GeneID" id="10527354"/>
    <dbReference type="KEGG" id="pgr:PGTG_20194"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20194"/>
    <dbReference type="HOGENOM" id="CLU_030195_3_0_1"/>
    <dbReference type="InParanoid" id="E3NXG3"/>
    <dbReference type="OrthoDB" id="2501761at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057194">
        <property type="entry name" value="DUF7872"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33339">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33339:SF1">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25278">
        <property type="entry name" value="DUF7872"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003177212" description="DUF7872 domain-containing protein" evidence="2">
        <location>
            <begin position="28"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7872" evidence="3">
        <location>
            <begin position="394"/>
            <end position="609"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="285"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="345"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP94262.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="609" mass="65981" checksum="FAFE7B0A3DB979B2" modified="2012-04-18" version="2">MANRNKSSMRRRTLLSVLILMTTTTMSMIEEGEERVPHNRTYTLQKRGGGLNVMGALGAGTGFSGGGSKSLKDPCGYIELTPDNWQDLGIDEYLANYPNGDKLTLQEFAAELNVPNFYCGVGMQCSAGQLCSPAVGINWIILYAIQEWSNFMNSLYKAVEHAVTLVKDAASSIVSDFTSLIPPNDQLYGWTVADIVCGVLGSISAIAVPAFIPADSLQVAMADLAVAPAFLATPELGIGRDKDSPREGQKAYDAAGAALDARNYKAFEKIQTEKDEQVIQDFFHPPSSLLLTNGPPKAPSDPASKEPIFHLQMPESPRDNSHPDSPFVESPPPSPNAQPGSSPFAPNPPQSPRYPGPAGADSTPPQNPKNDQQNPQTHQRQRRSLIKHKEGHSSSYAYTRYAYLESRLSSIQNRIQSYIAVNFKMATMAPILAKGGLAEILQGGAYLNPNPNENELQEGGKALAQITALAQLFQSLKIFVAIGSAECKAGGPNGAWNKKDELSYCTPDGLMMNIVQSSQTGLVIAMPNAHLLNEKYGYTTEFLARSAWECQKKYGVYTNSTAPPPVNMRSDCIFPLAVCDCTLPDVSKVFKGKNKNLIKACREVANLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>E3NXS7</accession>
    <name>E3NXS7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">t-SNARE coiled-coil homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_20332</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS989970">
        <property type="protein sequence ID" value="EFP94376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338795.1">
        <property type="nucleotide sequence ID" value="XM_003338747.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NXS7"/>
    <dbReference type="STRING" id="418459.E3NXS7"/>
    <dbReference type="GeneID" id="10527585"/>
    <dbReference type="KEGG" id="pgr:PGTG_20332"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20332"/>
    <dbReference type="eggNOG" id="ENOG502S813">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1062042_0_0_1"/>
    <dbReference type="InParanoid" id="E3NXS7"/>
    <dbReference type="OMA" id="PQSVPRE"/>
    <dbReference type="OrthoDB" id="244190at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019905">
        <property type="term" value="F:syntaxin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006887">
        <property type="term" value="P:exocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000201">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12791">
        <property type="entry name" value="GOLGI SNARE BET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="8">
        <location>
            <begin position="186"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="97"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="167"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="127"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="139"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP94376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="271" mass="30086" checksum="7E7D6C899BBC48E6" modified="2011-01-11" version="1">MTSTKPPTPARLSSISTSLLTSILELSQQNSLELTVNPATKSKILKNLLTLRDGLIQTGVDEQDQILVGLRNQYQRIVGLVRGVGGMETEIQGIDLSLPASKPPTTTTTTTTRTEEPLIDVEGTELEPTSSPVTSNLHQAQNTQSNSQSSMAIDIPSTTRTRSRKAIEEDEELMRSENEQVQRIQQVLLDDQDRTLDELSNAISRQRDLSLHISSELEVQENLLDELDQDLDFTSNRLTRANKRMDNLFKKIAKDGACWTIFGLVAILLVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPE7</accession>
    <name>H6QPE7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">EF-hand domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_20692</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EHS63598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890659.1">
        <property type="nucleotide sequence ID" value="XM_003890610.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPE7"/>
    <dbReference type="GeneID" id="13541507"/>
    <dbReference type="KEGG" id="pgr:PGTG_20692"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20692"/>
    <dbReference type="eggNOG" id="KOG4629">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014186_1_0_1"/>
    <dbReference type="InParanoid" id="H6QPE7"/>
    <dbReference type="OrthoDB" id="544685at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070588">
        <property type="term" value="P:calcium ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00051">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.10:FF:000674">
        <property type="entry name" value="Chromosome 19, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.60:FF:000006">
        <property type="entry name" value="Predicted mechanosensitive ion channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.10">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011992">
        <property type="entry name" value="EF-hand-dom_pair"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002048">
        <property type="entry name" value="EF_hand_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058650">
        <property type="entry name" value="Msy1/2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31323:SF11">
        <property type="entry name" value="EF-HAND DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31323">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN MSY2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13405">
        <property type="entry name" value="EF-hand_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25886">
        <property type="entry name" value="Msy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00054">
        <property type="entry name" value="EFh"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47473">
        <property type="entry name" value="EF-hand"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50222">
        <property type="entry name" value="EF_HAND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0106">Calcium</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="224"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="274"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="307"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="560"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="592"/>
            <end position="611"/>
        </location>
    </feature>
    <feature type="domain" description="EF-hand" evidence="8">
        <location>
            <begin position="507"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="114"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="369"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="776"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="38"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="69"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="133"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="392"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="425"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="810"/>
            <end position="828"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS63598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="843" mass="95068" checksum="6C441DADBF3E6FFB" modified="2012-04-18" version="1">MFRKLRPLDGKISSAFGLKNKKSQQSFKSTQSRHSAQEENQQMIPDGTHNQEQPVSPKHLDTTPPSKDTIGRRLQPDISREGQNDASAGSLASRYHRAMKEDVQSALNLAKEIGEETPLKTAGKGSSTSSSESDFDWNETDESELDESERQERKQRKLRQAQELEQSIHHAKRLRRLYLFLMKFSTPVRTTIIGVLGSGIAITPAILVWLKFTQLSTREHVLAWSLWLSISFATSCATSIIVDILPAIAIKLFELLYGAKPEKLKTQIELWMNVRYWTKVALGLTCYWVVLSLILSRMFRFYKDPHLAYFDWVMKVTAGLFTAGVVLLFEKILLQVIQLNFHRTSLKDRLEENERALWALDRLAAAKGVSHSPKKRNSKFLTSLTHHRTKSGRQTPGNKDSTIVDVPSTPKTPNMDSSADKRTAETSTSGGISSTQHKRNKSSNLLTVTDHLTSAINSALKHGTKGARGGMISSTHSAKKLAKKLFEGLDEDRGGVITRNEFEPYFKTASDAAMAFKLFDKDGNGDIDRKEMRNAVVRIYRERMSLAIGLKDMSSAVAKLDAVLISIASMLTIFIWLFIFNSKGTSSQLVPMATIILGFSFIFGNAAKNLFESMLFIFSIHPYDVGDLVAIDDVHMFVTEFGLFSTTFQRVDGQVVVAPNSLLISKKHILNIRRSGPMWETTEVMVGFDTPLEVLHEFRARLRQYVMDNPREWKGGLDVNIEFINNQNLIQLIIAMEHKSNWQDWGARWDRRTLLMKEMKRIMDSLNITYKLPTQPISLLPQKPGPRQRSSRREDTPVQSQHPPSAVPSRASSTNATPTRTNNPSHAFQSGHPFTSFRFPATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPK6</accession>
    <name>H6QPK6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20873</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EHS63891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890586.1">
        <property type="nucleotide sequence ID" value="XM_003890537.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPK6"/>
    <dbReference type="GeneID" id="13543206"/>
    <dbReference type="KEGG" id="pgr:PGTG_20873"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20873"/>
    <dbReference type="HOGENOM" id="CLU_052708_0_0_1"/>
    <dbReference type="InParanoid" id="H6QPK6"/>
    <dbReference type="OrthoDB" id="2506991at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="102"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="266"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="333"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="380"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="435" mass="49042" checksum="71D322D5244EA6F7" modified="2012-04-18" version="1">MASPSPTLADIIVQSSQGPKINPFVELIRGLDEKQNPPVDDSQTEGLLFSIATYIVVFLVCLAIISLPYLQGAGGHKRRHWVMKPQYLDGHPQPYWILNSGLIVAVSHLFGSTLFLVFLGLRYNAFKSGYAAESFYGSAWLELRWYPSYCSFFTQAWSTWFVRASDASLKGKSSGIHPLAFNINLIALPLTAFILALILISAQVASMSAQNNSYKDLISSLTVMSLQWHPGQNYLSSPLFHTASIQFLDYGTKSVHLLARFRTTGLFWTFMAVPTLIFYVIGISTLLRVMYKRFRAVKGESEHESTVSSFDSFPKRLMANRKRNKSSKLRNSFIYLGVHYTMMSLTLFYHIIIGLIFYFSDDVAMVNKSWLELFMVLSNSGSYLLLLALMVQLLRIISEGREQAGMVSKKSGRNAEYNDCISDSSRIHPDIKLMT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQU1</accession>
    <name>H6QQU1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21243</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EHS62870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890105.1">
        <property type="nucleotide sequence ID" value="XM_003890056.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQU1"/>
    <dbReference type="GeneID" id="13541498"/>
    <dbReference type="KEGG" id="pgr:PGTG_21243"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21243"/>
    <dbReference type="HOGENOM" id="CLU_2513738_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQU1"/>
    <dbReference type="OrthoDB" id="10300363at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="54"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="85" mass="9668" checksum="9A2E7032C5EECE1C" modified="2012-04-18" version="1">MVVELWWTRNFTNVFTGCRCNNNPFKVPLNTQIAQPQNKSEDSDSMESKESYEQKPTSLGRSSNKGRVEIPDEEAKRGSECGEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>H6QR34</accession>
    <name>H6QR34_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21301</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EHS63009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890013.1">
        <property type="nucleotide sequence ID" value="XM_003889964.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QR34"/>
    <dbReference type="STRING" id="418459.H6QR34"/>
    <dbReference type="GeneID" id="13540477"/>
    <dbReference type="KEGG" id="pgr:PGTG_21301"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21301"/>
    <dbReference type="HOGENOM" id="CLU_947097_0_0_1"/>
    <dbReference type="InParanoid" id="H6QR34"/>
    <dbReference type="OrthoDB" id="2551959at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266">
        <property type="entry name" value="CHROMOSOME 15, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266:SF1">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="294" mass="32900" checksum="FF37FB32ABB835E3" modified="2012-04-18" version="1">MRPPQPETCVKFGPSKISYLLPLCTHTCSSNIDTYLCQTSQKSNRKSQATQAKESDTKRARFKILHAEHKDPAQLFDLLSSTAGYIPDKSEVDKVREHNNQLDRVFGFLELSERMRAIVAAYWEAFTSGMQGTFASLQRKLELTNEADQDKITTLIRCAHSVRFPSDNPSGLSNEEMLAAIEGSTPPTKKILTKADRIEWSTAIVQEGFRAEYEGHDLIVLPTLKTLTEFAGLWQRNAYKAPYTSIVGPTMSGNIYWPAPSFRNCKLLPPTGGTEIRHAQALYSLIGCNPQHGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>H6QS51</accession>
    <name>H6QS51_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21698</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EHS63524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889669.1">
        <property type="nucleotide sequence ID" value="XM_003889620.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QS51"/>
    <dbReference type="GeneID" id="13542862"/>
    <dbReference type="KEGG" id="pgr:PGTG_21698"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21698"/>
    <dbReference type="HOGENOM" id="CLU_1856279_0_0_1"/>
    <dbReference type="InParanoid" id="H6QS51"/>
    <dbReference type="OrthoDB" id="2508431at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003605767" evidence="1">
        <location>
            <begin position="31"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="138" mass="16085" checksum="44A43AE662DE2F64" modified="2012-04-18" version="1">MRLPKPKPLGLIIWRLINAICHVNLQGARAEKREDKIVMETEELMNFILAELFRPQAHYPILGRVLQLKDNPLDWFSDTQFLLLEMLSSMLSHPNPAHKDIDTVLNFKTPDIDLLSQFVYLWNSNHNPHFVQENLSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSU2</accession>
    <name>H6QSU2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Vacuole protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_21874</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178299">
        <property type="protein sequence ID" value="EHS63835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889434.1">
        <property type="nucleotide sequence ID" value="XM_003889385.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSU2"/>
    <dbReference type="GeneID" id="13540591"/>
    <dbReference type="KEGG" id="pgr:PGTG_21874"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21874"/>
    <dbReference type="eggNOG" id="ENOG502QVFG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_028299_0_0_1"/>
    <dbReference type="InParanoid" id="H6QSU2"/>
    <dbReference type="OrthoDB" id="2128042at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031606">
        <property type="entry name" value="Kch1/2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36424:SF1">
        <property type="entry name" value="LOW AFFINITY K(+) TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36424">
        <property type="entry name" value="PHEROMONE-REGULATED MEMBRANE PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16944">
        <property type="entry name" value="KCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="39"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="87"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="211"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="543"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="560"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS63835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="569" mass="64397" checksum="11F2EF9A2D22B3B8" modified="2012-04-18" version="1">MCCSGPQWKREEVPDHKWDFVDVREFHTSGLMSYFQYSFLYVLVIKSVLVYASDIYTMVLLLASNHWAGSILESSAGRSAMNVPFKVGKWIFFGCIIFSFLLLFWEAKKSRAIIKSRDISYAFTNVMANNYYSMKSYDHFCFFSQINNSKKKKDDFAFFVFFTFKGWKRLLLADAPRQVINGITLYSFARSVNFTTDISKWYEGSFAKASVLLSMAFTVTIWVGSFLLLVAAALLYVPLLCYIQGNLKEYCCHKVDKRIAELMKRKHRKRLAKEAALMRKEAMGDYSHLKDKKGQFKAAPLPQPTLPQIGLHELISDTKSERTGTANSIRTVSRAAQHPYPSSLNHHYPASNPGGGLGMAYNVVNRWPSEQEYSEKVGYAESIASTGRFVAHQAPTAGSVYGHNGQIHGARTHPYGLHPLAHQLPSDTHSSLNHAPSYSSEEQQYYTNDRHAVTRRSIAITSDYGGYGSEYGGYAHEPPLPTGQYHSEAPYTEFDFGSLPNHLESAYNPPIQHEHRFDYPEEGPPLVDREIMELYGTGALERFDQASEAATSIPPGRYPQHTNHPYSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSX3</accession>
    <name>H6QSX3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21945</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178301">
        <property type="protein sequence ID" value="EHS63927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889395.1">
        <property type="nucleotide sequence ID" value="XM_003889346.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSX3"/>
    <dbReference type="GeneID" id="13540671"/>
    <dbReference type="KEGG" id="pgr:PGTG_21945"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21945"/>
    <dbReference type="HOGENOM" id="CLU_2795165_0_0_1"/>
    <dbReference type="InParanoid" id="H6QSX3"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="68" mass="7779" checksum="A2BE5D0D883388C5" modified="2012-04-18" version="1">MSWPVRTVAFNVGLRLTNTAHRRQVQLGRKGWPDKHRREDDELQDAGFHSFSKIRLDKLESNSGGGCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>H6QT35</accession>
    <name>H6QT35_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21992</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EHS63989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889351.1">
        <property type="nucleotide sequence ID" value="XM_003889302.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QT35"/>
    <dbReference type="GeneID" id="13541899"/>
    <dbReference type="KEGG" id="pgr:PGTG_21992"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21992"/>
    <dbReference type="eggNOG" id="KOG3105">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1058216_0_0_1"/>
    <dbReference type="InParanoid" id="H6QT35"/>
    <dbReference type="OrthoDB" id="2506894at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="140"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="161"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="263" mass="30066" checksum="E0C9A49263B78718" modified="2012-04-18" version="1">MIPSFIQLIKDNPNSIHLSCVGDTLYAHNLAERPSLNDATKDCEDVKLSTIENYFKEMKIKNKEKQDALQLKIKKGQQITTEEEEWMDGDGNLIDAVLLMEKLGELASTKKTINLRSSEINIFLRILEFCSQEMSSKSLLNKKNNNKNSNRNPTEKPSNPKKTEKNSDSNKTKTPSIPNKKRKADNDEPKSKSKISNASYAEKVEVLDWHHRNGKNQSKTAAHFEEIYPHLKIKQPLISKWLKDETSIRSKNSESNNPSSKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTB7</accession>
    <name>H6QTB7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22028</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178310">
        <property type="protein sequence ID" value="EHS64071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889250.1">
        <property type="nucleotide sequence ID" value="XM_003889201.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTB7"/>
    <dbReference type="GeneID" id="13541681"/>
    <dbReference type="KEGG" id="pgr:PGTG_22028"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22028"/>
    <dbReference type="HOGENOM" id="CLU_003292_8_0_1"/>
    <dbReference type="InParanoid" id="H6QTB7"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052055">
        <property type="entry name" value="Hepadnavirus_pol/RT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050:SF7">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="291" mass="33375" checksum="CAB0EAA620D8F7A4" modified="2012-04-18" version="1">MNIDIWQEALSKAGLLPVMNHIIDGFINGFDQGIPDHVIGNLRWFTPDNHSSAINAEDKIQKSINEEVEAKRMFGPFSHEEVAARFKFFRSSPLGSVVNADGKMRPINDLSFPKKNDKKNIGIMSVNSFVNKLDFKTTWDDFTIVSNFFRNNEDKFDLALFDWAKAYRQIPTKSSQWPFLMVKDLKGDLYVDTRITFGGVAGCGSFGLPADTWKQIMESEFDVKKIFRWVDDNLFVKKTDSLCNMKAVSERSVELGVATSVDKCTEFAREQKFIEIFYAIEVRSNKRYVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTD3</accession>
    <name>H6QTD3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_21902</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178311">
        <property type="protein sequence ID" value="EHS64152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889232.1">
        <property type="nucleotide sequence ID" value="XM_003889183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTD3"/>
    <dbReference type="GeneID" id="13541704"/>
    <dbReference type="KEGG" id="pgr:PGTG_21902"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21902"/>
    <dbReference type="HOGENOM" id="CLU_040082_0_3_1"/>
    <dbReference type="InParanoid" id="H6QTD3"/>
    <dbReference type="OrthoDB" id="2505476at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003606564" evidence="2">
        <location>
            <begin position="21"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="225"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="234"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS64152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="285" mass="32381" checksum="1C8F8EC8C1858B9B" modified="2012-04-18" version="1">MEPNILTAIIATSIAVLLLANQFQKGQPYYSNGGRATYVKFVLLSAQDKLFHKITLMMHPTFDALVELLRDTPMRETAVRFRRGLFTVNRYFTEVLEALFILYPKYVKLDPQTCAQPSDLKDDPKYKAFKNAVGAVDGVLIMLESPQMNSHPGDAARAGWEGSTHNSRVYMDAYSKGLALPGNKYPTRNMAFKKVKLKKQVQLVYSLCMLWNFICKHEEFDPFEDQVDPDEVPSANSNSPLSQTAKCDSTPHRTNTQETTAMKLQQHHIATKLWDQYVSYNSRDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTJ1</accession>
    <name>H6QTJ1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178315">
        <property type="protein sequence ID" value="EHS64206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889165.1">
        <property type="nucleotide sequence ID" value="XM_003889116.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTJ1"/>
    <dbReference type="GeneID" id="13541222"/>
    <dbReference type="KEGG" id="pgr:PGTG_22130"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22130"/>
    <dbReference type="HOGENOM" id="CLU_003292_5_0_1"/>
    <dbReference type="InParanoid" id="H6QTJ1"/>
    <dbReference type="OrthoDB" id="2503657at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052925">
        <property type="entry name" value="Phage_Integrase-like_Recomb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34605:SF3">
        <property type="entry name" value="P CELL-TYPE AGGLUTINATION PROTEIN MAP4-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34605">
        <property type="entry name" value="PHAGE_INTEGRASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="370" mass="41771" checksum="A6F26FFB6AEFB6ED" modified="2012-04-18" version="1">MLDNSEDIVIIDNERLTVGHLNKIKAFAANGTAHYPISTLNAHLLSGWKWNTIVGYNAAVKKFIFFKNLSGNHNFKLPASDKDIEDFCFWAGRNLYLNNSYKILAGSIKKYLCGLKAWHTFHGVEYPDVSDKRIELLLKASSKVDATLPTKPKKPPIMLKHLIWLSNALFPGGDIDRTIMDLAIVAFWGMARIGEFTYENTLGDLNKENSILTSDVRIIKSAIGEKAILTVRNAKTAQPGKPQQIIVHSLNNMICPVLAIKRRLDEANGNETSLFGFYREGTRRHLTRTIAVNRIKLVLRTGGFEGLLGHSFRVGGASLRYALGTPVEEICLLGRWISNCYKLYIREYSDEDTQESKRIISELNELWEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUC1</accession>
    <name>H6QUC1_PUCGT</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22373</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178335">
        <property type="protein sequence ID" value="EHS64584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888891.1">
        <property type="nucleotide sequence ID" value="XM_003888842.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUC1"/>
    <dbReference type="GeneID" id="13542390"/>
    <dbReference type="KEGG" id="pgr:PGTG_22373"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22373"/>
    <dbReference type="HOGENOM" id="CLU_478275_0_0_1"/>
    <dbReference type="InParanoid" id="H6QUC1"/>
    <dbReference type="OrthoDB" id="2507135at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="228"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="437"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="232"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="437"/>
            <end position="449"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="570" mass="62930" checksum="619B6FD7D3576DA4" modified="2012-04-18" version="1">MEHQIVPPSSHSQQGSASESSTIATRPIHSRSASHPIKTAFLTAKKAIHYQLSNPNLATPQSTTADNPSILLPALDPLNADSMQKTSDPLRFKSTLFSSPSNPEISVGHISRRLLEPIRPLQTPHRETGQLEAIPTSLARNIPTRPTRSNVAHNELMVRGPFPVIVSSVAHPSSTKKRSHRRRWTTQRSKDDNPLNSNDSEPFNLSLLNQQALVSSENGRSTLVADQHSDDASNNISPQSPQTSDHSDTDCNQLTAPYPIGNLDYNDQESFDIEDLTTPTLKSCERFEDDNSITPCQTFTTPSFKKLQQGLKVTPRMIYEDYQKKFNEEDLMIKSRDLKVSPRHENPHLTIGKNNFDNDYGNAAHVIVSDDSTASAAPHGTSLDETSLQDAADESFESFGDDQFFGPLFSSKSRTTRKRNSELIGVFPLPPATRNLSCLPSNVQRTPSPKQMAVPGSSKASTPMIPRIVMGGNEKEPARSRTSSPLVLGKPLRANASTNQCTGKNTPFQLQPSPYRHVEHDSIFHDGPDHLESSTRNMFDSFLERSPKGSGSKDSVQIFSFEKVVQKPQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUI2</accession>
    <name>H6QUI2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22403</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178342">
        <property type="protein sequence ID" value="EHS64694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888829.1">
        <property type="nucleotide sequence ID" value="XM_003888780.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUI2"/>
    <dbReference type="GeneID" id="13542461"/>
    <dbReference type="KEGG" id="pgr:PGTG_22403"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22403"/>
    <dbReference type="HOGENOM" id="CLU_990902_0_0_1"/>
    <dbReference type="InParanoid" id="H6QUI2"/>
    <dbReference type="OrthoDB" id="10273800at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="18"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="281" mass="31332" checksum="788BE40F3F4F25E5" modified="2012-04-18" version="1">MGPEDYSTYLDGVDQITNWLDDQPSDNDALASESRSVSVVNTAANTQQEEPNSEDKGKNKSEDFKVSIEYKLYVALKKPKPTTTRAGRKAVASETNDKYSKLVSKPNKLSFTWNASNTNLDVFKEAVLEILRALEPSQVFGLAEKQEKAKNLCWYAIIPYGGQFVEKNQTMLDNSKIFLKFLEVAEGKGEAKIHLVQNDPKAIAERDKALKQLSEQEKANDEDEAPSSEPTAGASLNKKIIDNMWLIRATHMPKEDLTGSMELPVMVDPADSRRFIALSPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>H6QV96</accession>
    <name>H6QV96_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22692</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178387">
        <property type="protein sequence ID" value="EHS62797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888570.1">
        <property type="nucleotide sequence ID" value="XM_003888521.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QV96"/>
    <dbReference type="GeneID" id="13540779"/>
    <dbReference type="KEGG" id="pgr:PGTG_22692"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22692"/>
    <dbReference type="HOGENOM" id="CLU_2441935_0_0_1"/>
    <dbReference type="InParanoid" id="H6QV96"/>
    <dbReference type="OrthoDB" id="10341915at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003605831" description="Secreted protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="90" mass="9994" checksum="188389B5C58A0B6D" modified="2012-04-18" version="1">MFQIPARLILMCAVALQSLVWAPTVPTRGHSLTQTTSIEPPRPEQIEQMNFESAVKAGRACECHTPGLYQTLSRVSRRKTFTTGYNVCLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3JPY3</accession>
    <name>E3JPY3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307072.1">
        <property type="nucleotide sequence ID" value="XM_003307024.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JPY3"/>
    <dbReference type="STRING" id="418459.E3JPY3"/>
    <dbReference type="GeneID" id="10527517"/>
    <dbReference type="KEGG" id="pgr:PGTG_00022"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00022"/>
    <dbReference type="HOGENOM" id="CLU_395946_0_0_1"/>
    <dbReference type="InParanoid" id="E3JPY3"/>
    <dbReference type="OrthoDB" id="2499763at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="528"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="581"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="606"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="633"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP74066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="711" mass="79959" checksum="00C0BC223797662B" modified="2011-01-11" version="1">MALSTSQSILEYTRHHNLTCDPDWTFEFAEDLVIEFSIAFKLAPPTQSLDEDIKHTWSSLDLITSQNGKENYKQLESLDSWLIPQGRLAYAKVVRDLVRRYKHLDLADPDWWGDGLFEPQLTPIMDDYILARRNTKATPISLQPDDLNTIKNWISFDGRQTFTDLFADGEKPIHIPTPEELSETTLQLSSLQYQKMKENKVQRGEVVRDFSPLVSMASDLLESDETLPSETSRHAISPPLIARQSSTSADSWRIKGKDFVSSVISNLREEEIRQFSPTLSNDAWNDIASLVLREVFAPPSLLDSCLRPSCTPPSSPSPHESNEQKDVAIEFLSSSADPNNWDKLEGLDNPFETVLFSRTEGMPLHLDRSTSFNPRKHWDMASCLFDEHQPARASITPEPAFEGRSKEGNQDLLEILNQLVANRGWDDQQFEHEIFGSPGEAVNLHSGLKRIPIDEPDWPIMKHEISEELLPSNLDEFRLREPCSIRSKNTTTISRGLQKVEGLKALNIELPWNAYSFLPSTTLENLTDLDSKPVDDVSTDAGSSSKDGYSFISKRLKSEHPHRDADEPWIKLSDFIDPSSSSENESYCSQLDLDGKNESGEVHQGAVSTPESTKRSTSPTIRTDPDFGKVASPTESSRVPNHSSQSNPSDTDCYPRGFEVQSDFGTQNGNSDCTEDDEFIDLLGLRSVDSSADIQNHGFYGVWTMSEPPNM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQ78</accession>
    <name>E3JQ78_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Peptidase S9A N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_00324</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74368.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307374.2">
        <property type="nucleotide sequence ID" value="XM_003307326.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQ78"/>
    <dbReference type="STRING" id="418459.E3JQ78"/>
    <dbReference type="GeneID" id="10527695"/>
    <dbReference type="KEGG" id="pgr:PGTG_00324"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00324"/>
    <dbReference type="HOGENOM" id="CLU_964922_0_0_1"/>
    <dbReference type="InParanoid" id="E3JQ78"/>
    <dbReference type="OrthoDB" id="248387at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070012">
        <property type="term" value="F:oligopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.120">
        <property type="entry name" value="Prolyl oligopeptidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023302">
        <property type="entry name" value="Pept_S9A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051167">
        <property type="entry name" value="Prolyl_oligopep/macrocyclase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42881">
        <property type="entry name" value="PROLYL ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42881:SF2">
        <property type="entry name" value="PROLYL ENDOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02897">
        <property type="entry name" value="Peptidase_S9_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50993">
        <property type="entry name" value="Peptidase/esterase 'gauge' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase S9A N-terminal" evidence="2">
        <location>
            <begin position="54"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP74368.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="312" mass="33295" checksum="11CB31EC5A0DA73D" modified="2012-04-18" version="2">MSWYEYPLVGRNPSRQEEVFQSKQNGSVTVPDPYSWLHEPPKQSNALKRATDPKQPAGKLFLDPNLFSINGTTALSFSATSESGIYMAYGVSRSGSNSQTIYVRRTDSPHTKSAADGGKRGKDTVVAGRCTYPPACREVYIPTSSLGGIPPGKLVYIPACWKGFLPTSWCVHTSLSEGIPSDELVYIPSRREGFLPTRGYSSQLVGRNPSRSPGGIPPGKLVCTPARQEEHLPASCLPGGIPPGGVASLGTGYPLGYPDICQVWVEKQPSKAKSAPAGGYPLVLAGIHQRIADICNGLSPPISSHGLGLGRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQU3</accession>
    <name>E3JQU3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_00271</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307321.1">
        <property type="nucleotide sequence ID" value="XM_003307273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQU3"/>
    <dbReference type="GeneID" id="10527968"/>
    <dbReference type="KEGG" id="pgr:PGTG_00271"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00271"/>
    <dbReference type="HOGENOM" id="CLU_143009_0_0_1"/>
    <dbReference type="InParanoid" id="E3JQU3"/>
    <dbReference type="OrthoDB" id="168171at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324:SF2">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP74315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="140" mass="16283" checksum="6440A6A8E3732346" modified="2011-01-11" version="1">MSNPFQIKGITWYYDQIDDQGFCSMQVLLSWLAAPGNYQRWCHAPSKLPLCAEVLIDMQDETIYHQNETEIEAVVEYLEESFRIAKQYYMRIMPTLVATNPSDGWRIAQGAKKVIKRCEHWIILNEIMGGLPTTHPIIYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3JTG5</accession>
    <name>E3JTG5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01883</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75290.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319709.2">
        <property type="nucleotide sequence ID" value="XM_003319661.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JTG5"/>
    <dbReference type="GeneID" id="10542756"/>
    <dbReference type="KEGG" id="pgr:PGTG_01883"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01883"/>
    <dbReference type="HOGENOM" id="CLU_2265044_0_0_1"/>
    <dbReference type="InParanoid" id="E3JTG5"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75290.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="103" mass="11500" checksum="7351CC831A2AA73E" modified="2012-04-18" version="2">MLRDPPEPLQVPPRPRIVGDPPHPRPLPKAPAAFQTPPEAPTVQRSAPGRPRAALELQRPPQRAPRPPNNLPERPRTLKRPLRGPHSPTKAPWTPGKEEVEDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUM8</accession>
    <name>E3JUM8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">DNA-directed RNA polymerase subunit</fullName>
            <ecNumber evidence="14">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGTG_01084</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the RNA polymerase III (Pol III) complex consisting of 17 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
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    <dbReference type="EMBL" id="DS178264">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="XP_003320172.2">
        <property type="nucleotide sequence ID" value="XM_003320124.2"/>
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        <property type="interactions" value="664"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JUM8"/>
    <dbReference type="GeneID" id="10543745"/>
    <dbReference type="KEGG" id="pgr:PGTG_01084"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01084"/>
    <dbReference type="eggNOG" id="KOG0261">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_000487_3_0_1"/>
    <dbReference type="InParanoid" id="E3JUM8"/>
    <dbReference type="OrthoDB" id="270392at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
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    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02736">
        <property type="entry name" value="RNAP_III_Rpc1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02583">
        <property type="entry name" value="RNAP_III_RPC1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000019">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
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    </dbReference>
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        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.30">
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
        <property type="entry name" value="RNA polymerase ii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.274.100">
        <property type="entry name" value="RNA polymerase Rpb1, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042102">
        <property type="entry name" value="RNA_pol_Rpb1_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035698">
        <property type="entry name" value="RNAP_III_Rpc1_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035697">
        <property type="entry name" value="RNAP_III_RPC1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015700">
        <property type="entry name" value="RPC1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006336">
        <property type="entry name" value="PRK08566.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48446">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA' N-TERMINAL SECTION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48446:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA' N-TERMINAL SECTION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14 16" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="15">
        <location>
            <begin position="259"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00058108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EFP75753.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1419" mass="157964" checksum="CCF4DD75733B0697" modified="2012-04-18" version="2">MKQIVLQEAPQKIKHIQFSVLSSQEIVALSEYESTQRDLYSMVGSENNGISIPGAIEPLVKKPAKGGVLDSRLGTSDKSGICGTCNAKMADCVGHYSYIKLILPVFHIGYFRAVIQMLQDICKTCARVLLSEEDRRLYLIKFRRPNLENLQRQHLAKQVNTACRKIITCPYCGATNGTVKKVGALRIIHEKFRAKKKADELAAFRDTFITAVKETKELGPHLHKAQEDMNPLRVLNLFRRITPSDCELLGLDPEVGRPEEYIWQYIHVPPVCIRPSVAQEAATNEDDLTVKLTEIIFTNTLIKTGLVKGVPTQNIMEQWEFMQLSVAMYINAELPGVPSQMGVKPIKGFCQRLKGKTGRFRGNLSGKRVDFSGRTVISPDPNLSITEVAVPDRVAKILTYPERVTAHNIDRLRQCIRNGPDIHPGANYLVAGSTTNASGSALPFKRSLKFGNRNHVANTLRLGDTVERHLHDGDVVLFNRQPSLHKLSIMSHRAKIRPWRTFRFNECVCTPYNADFDGDEMNLHVPQTEEARTEATELMNIKYNLVTPRNGEPIIAATQDFITASFLLSRKDNFYDRQQFSQILQYLSDATLHIDLPPPVIWKPVRLWTGKQVFNVLMRPNKDSPVKVNVEAKCATHQKAAPEYLPDMSPNDGWLVIQNSEIMCGVVDKAIVGGGKKDSIFAVINCDFGPDEAVKAMNRLAKVSARWLANMGFSIGVGDVTPGERLSTQKNALVAKAYKETDELIQQSKAGKLETLPGRDLDQTLEAMISGVLSKVRDSLGEICMAELTRHNAPLIMATCGSKGSKINVAQMVACVGQQIISGNRIPDGFQDRSLPHFHKKAKEPPAKGFVRNSFHSGLRATEFLFHAISGREGLVDTAVKTAETGYMQRRLMKALEDLATRYDDSVRNSVGGVVQFTYGDDGLDPTYLEGDGEPVEFARTWRHTKATVSHLDGRGLHPYEITQLVTDGLAQPRFTTACTQRFRDSVLDFMVERVVKPAANFRQSYGMYPALELEGDWDEYTDLSAGVSEASKKIVINKTQVTLKQIETFLDHCWKKYVKAKVEPGTAVGAVGAQSIGEPGTQMTLKTFHFAGVASMNITLGVPRIKEIINASKLINGPIINAPLVANHNERSARIVKARLEKTYLGDVTSVIEEMYSNDSMYLGIHIDMETVQRLQLEVTLDSIKWSIINAPKLKITADQIQIIPRKNRIRVYVDTNLADGEKVLPFYQKLMFLRRVLPGVVIKGLPKAGRTVINKNSKNEYELLVEGYGLLDVMTTDGVVGTHTMPNHIMEAQSVLGIEAARNCIIKEISYTMDSHGLKVDPRHMMLLGDIMCYKGEVLGITRFGIAKMKDSVLMLASFEKTTDHLFDAALYSKRDAIEGVSECIIMGNPANDIGTSAACLVSEVPKLPPRRKVLFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3JVA2</accession>
    <name>E3JVA2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_01308</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75977.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320396.2">
        <property type="nucleotide sequence ID" value="XM_003320348.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JVA2"/>
    <dbReference type="GeneID" id="10533057"/>
    <dbReference type="KEGG" id="pgr:PGTG_01308"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01308"/>
    <dbReference type="HOGENOM" id="CLU_927914_0_0_1"/>
    <dbReference type="InParanoid" id="E3JVA2"/>
    <dbReference type="OrthoDB" id="2498976at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="233"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="245"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP75977.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="300" mass="33661" checksum="1C8DE483CA398C07" modified="2012-04-18" version="2">MFHSSSEDRTDIFNWASKLSLVGVLEQHGSRWAVFSVALSLVLALPSVDGKTHRTDLPDNGFRFLPPDVEKVIPSSVPLLFDRASEVEGNPSALTNSFIAFSTVLPIMAVLLAVLSWKRRRKDRELYESVLQTYHTRKELLNRAERLNVDVYEKNTQHEQWTSIQFPPQTESGPELRDSKRGNENQSSKNERNARPMQPPNATVANQCPEVAVSIPPLPQDMLETTIAAERILPLPTGSDPPGSALQSGKRTSSENSSPQDKPNLPDHEIEDFPIPTNRKTRVDSIIVYCEKQKKLSRES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWJ9</accession>
    <name>E3JWJ9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Aquaporin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_02865</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the MIP/aquaporin (TC 1.A.8) family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320843.1">
        <property type="nucleotide sequence ID" value="XM_003320795.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JWJ9"/>
    <dbReference type="STRING" id="418459.E3JWJ9"/>
    <dbReference type="GeneID" id="10534479"/>
    <dbReference type="KEGG" id="pgr:PGTG_02865"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02865"/>
    <dbReference type="eggNOG" id="KOG0223">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020019_1_4_1"/>
    <dbReference type="InParanoid" id="E3JWJ9"/>
    <dbReference type="OMA" id="LALNTMH"/>
    <dbReference type="OrthoDB" id="3222at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015250">
        <property type="term" value="F:water channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006833">
        <property type="term" value="P:water transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1080.10:FF:000014">
        <property type="entry name" value="Aquaporin 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1080.10">
        <property type="entry name" value="Glycerol uptake facilitator protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023271">
        <property type="entry name" value="Aquaporin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034294">
        <property type="entry name" value="Aquaporin_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000425">
        <property type="entry name" value="MIP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139">
        <property type="entry name" value="AQUAPORIN TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19139:SF199">
        <property type="entry name" value="MIP17260P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00230">
        <property type="entry name" value="MIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00783">
        <property type="entry name" value="MINTRINSICP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81338">
        <property type="entry name" value="Aquaporin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="123"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="165"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="191"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="234"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP76424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="337" mass="35842" checksum="8C7A9597EC395139" modified="2011-01-11" version="1">MKAQNRPEASLSQYFKSFAEPHMFSQIKIDLIAAVGEFMGTFVFLLVGLGGIQAGKTSNTDNSISTTGNSTVPNLNLLMYVSTSMGLSLLFSCWIFFRATGAAFNPNVSLALLLIRIISPLRFVLYTTAQLLASIAASAVLQAILPGPLAVSTTLGAGTSAAQGLFIEVFITCALVLSVLFLAVEKHKATFLAPIGIGITLFAGHLFAIVYTGAGMNTARSFGPAVVSGFSPEHWIYWIGPTLGSLLAVGIYAFMKLFKYWKLSEGQDTDIPSKSPELFTQAAPPVIEVARPANPEKNEVGAQAGASKPAKVFWPPQSPSMTLSHQKNFSHDGAGMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWX4</accession>
    <name>E3JWX4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4219 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_02010</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320968.1">
        <property type="nucleotide sequence ID" value="XM_003320920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JWX4"/>
    <dbReference type="GeneID" id="10528414"/>
    <dbReference type="KEGG" id="pgr:PGTG_02010"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02010"/>
    <dbReference type="HOGENOM" id="CLU_103048_0_0_1"/>
    <dbReference type="InParanoid" id="E3JWX4"/>
    <dbReference type="OrthoDB" id="2503017at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP76549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="235" mass="26439" checksum="42E0BAFF374B56AB" modified="2011-01-11" version="1">MEKINSTILKTALKAIPLLTANNYTLWQNCIDDMLDLQGLWDSLTSEKDSSIHPNIINHDNKKDAQKIWSSISDFFASTQPTNCARVFNELLDLSFNSNNVQPFITLVRTINSQLFKIGIDLPHNLVTYILLKKLCPSLKNISQKITHSDKPLTSNLVLDHLKLFNNNQAAIAARNTGSKTEVVALYSNFSKKCKIMAHNFLSNHPGSSCWMLYPHLRPANGVKANQSESTKKDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3JYI1</accession>
    <name>E3JYI1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03062</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321525.1">
        <property type="nucleotide sequence ID" value="XM_003321477.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JYI1"/>
    <dbReference type="GeneID" id="10537316"/>
    <dbReference type="KEGG" id="pgr:PGTG_03062"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03062"/>
    <dbReference type="HOGENOM" id="CLU_1090441_0_0_1"/>
    <dbReference type="InParanoid" id="E3JYI1"/>
    <dbReference type="OMA" id="NCEIAYH"/>
    <dbReference type="OrthoDB" id="2506744at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="262" mass="26904" checksum="763FADDAD3196FD7" modified="2011-01-11" version="1">MDCRFLRSTSMRKANSNQMYTFLSITILANLFCFPFSSCQLPAPGITPSSNLALTTSPPGQTTPPGTTNTGAGIPGTNTGAGVPGTNTGAGISGSTTTLATTSTPGTTTNGNPSTGRTTTNPSPKADSLYSCSEAFAPLDEDQTLVFDRPVPEAAGYCKSNASSDNLACALRTCVAFPTCSGCSVVTSSATDSKVTTDGRVLPQPQNCELAYYISHKPGVPSLCLTAQTEVLQCTGGCQNATNCGTCFKVTETNQPSKGPTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3K106</accession>
    <name>E3K106_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_03937</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP77981.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322400.2">
        <property type="nucleotide sequence ID" value="XM_003322352.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K106"/>
    <dbReference type="GeneID" id="10541289"/>
    <dbReference type="KEGG" id="pgr:PGTG_03937"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03937"/>
    <dbReference type="HOGENOM" id="CLU_863664_0_0_1"/>
    <dbReference type="InParanoid" id="E3K106"/>
    <dbReference type="OrthoDB" id="2504808at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="183"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="238"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="248"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP77981.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="322" mass="35639" checksum="CBD3491BB573235E" modified="2012-04-18" version="2">MPPGPGGTDSRDARGPPETHRGSVRVSVNEPYLTSSDTLVLGNSLKRDMPSETFELEVPFHPVSPLPPAQRPPGVAGGHRYRAASAVTLSQLYSCIPDRFRHPYTDPPTKNYHLLRFTQCLTPKFPTCQFNCLWTKYPSRTRSTSVEARMVVLHDPNSKGPQISLFLHRRTPPASSELLSATLGVYIIFALVVVIIGSLLLVVVLQLQSRRIEERLQAVIAAHQPRQPRPRTRASMPTPCPGIPPSRRPAASFPSVSCRGSIPDSSSSDDSPNTDFTVPHRIPNQLLSFNEKSTRWSHNSLTFKFLDEPYSPKSPESAHSLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3K167</accession>
    <name>E3K167_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Wax synthase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_03998</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Secondary metabolite biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the wax synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP78042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322461.1">
        <property type="nucleotide sequence ID" value="XM_003322413.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K167"/>
    <dbReference type="GeneID" id="10541351"/>
    <dbReference type="KEGG" id="pgr:PGTG_03998"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03998"/>
    <dbReference type="eggNOG" id="ENOG502SAAK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030439_0_0_1"/>
    <dbReference type="InParanoid" id="E3K167"/>
    <dbReference type="OMA" id="IAPIAFW"/>
    <dbReference type="OrthoDB" id="1077582at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044851">
        <property type="entry name" value="Wax_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032805">
        <property type="entry name" value="Wax_synthase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31595">
        <property type="entry name" value="LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31595:SF57">
        <property type="entry name" value="OS04G0481900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13813">
        <property type="entry name" value="MBOAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Wax synthase" evidence="8">
        <location>
            <begin position="298"/>
            <end position="376"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP78042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="468" mass="52558" checksum="EB850E73CEBD495A" modified="2011-01-11" version="1">METINNTTLILSMQSFTSSTPWFTSNQTSSSWGTSQPAQGVLTRWLFEKVLFLSLMVVQCALLHPQFQHSSSARLSRLSLGPPIIGWWLCYPFRLPLLPFQDRNIFPGFLAAIMVFKSLEWTFSPGPYHIRTLKIVDGTPVWEKESTTKISSENSQSNNLADLALWTTLQFTSQRGFRWSWGPAAKGNENSFLQALLEIVRLQLVLLPCLGFVLYSQDWDSYDFDPRKPLLSLGVPSFRGLGLLAGGIHSACTMFVISCSIEMISALPVLSTYLLYPAAKRIGLPSKLVELANPANFPPQFGSLFELSSLAHFWGKSWHQKFRRPFLFCGGKPAMSIARALGGSANVQKISGVMGVFALSGLFHEYPMYAFQRAPHPYPRALFETLPASFLFFFLQSFGVILEPLIIPYVPRRLGGAKLWTAAFLLLTAPLFTRDICRPAGMFNQYRPPHMWTWMDVVIPVPIAARIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2B4</accession>
    <name>E3K2B4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dynamin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_04439</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322902.1">
        <property type="nucleotide sequence ID" value="XM_003322854.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2B4"/>
    <dbReference type="STRING" id="418459.E3K2B4"/>
    <dbReference type="GeneID" id="10536164"/>
    <dbReference type="KEGG" id="pgr:PGTG_04439"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04439"/>
    <dbReference type="eggNOG" id="KOG0446">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_376041_0_0_1"/>
    <dbReference type="InParanoid" id="E3K2B4"/>
    <dbReference type="OMA" id="VIANQFK"/>
    <dbReference type="OrthoDB" id="2583551at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:004690">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF21">
        <property type="entry name" value="DYNAMIN RELATED PROTEIN 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="3">
        <location>
            <begin position="32"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP78483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="629" mass="71763" checksum="DBE7DCD9E6736CE3" modified="2011-01-11" version="1">MSTSADTTPAISCHRSHLDKVDQIRKLGRMDRISLPQIVVVGDQSSGKSALLEMISGVTLPKDSGKCTCFPTEVSMRPAEEFSAKVLIEGKIDSRVRLPKSREEVAEVIEDAKKLIMEGEDQDISEKVLTIELSGPQLPMLTLVDLPGYVQTYSQGQSATLARDIEKLVEKYLLEPRTIILAVIPVNRDFETNVAIKHIRDFDEEGKRTMCVLTKPDLLDLGTEGRVLEILAGKKMHLSRGYHIIKNKNFEDCRAGDSRDVTLRKESIFFKRSPWSDISVTNRGIPKLIERLTNMLSKQIEKEFFGIKKDLIKNKHELEEQLTQLGPGLSTDIEKLNLLNRNINLFMKQFKNLVDGNYSEGGFDQECFIRATVQNLHQGFHREIIKITNVAVSSLDVAKVMEATRGRELKGMLQFQPFVILCRQVVQKWLSPTERHIEEICQLASDVSVEITEKECNPILNRYISERMSEFFDQQLKRMHTEASELLNDELSSPFTLQDLNVGKHWRFEDTFPSIPTFNFGVGVAKPPASNSRSTTNEPEVIDYSKVKSFLREYCLTAANRYIDAICLYVIERGLFRNCYSRGIKWFEDDPLALSQFQEPQESSEWREKLPIEIKRFKDAIAILGQPTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2C4</accession>
    <name>E3K2C4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Lactoylglutathione lyase</fullName>
            <ecNumber evidence="3 9">4.4.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Glyoxalase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_04449</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 9">
            <text>(R)-S-lactoylglutathione = methylglyoxal + glutathione</text>
            <dbReference type="Rhea" id="RHEA:19069"/>
            <dbReference type="ChEBI" id="CHEBI:17158"/>
            <dbReference type="ChEBI" id="CHEBI:57474"/>
            <dbReference type="ChEBI" id="CHEBI:57925"/>
            <dbReference type="EC" id="4.4.1.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit. In the homodimer, two zinc ions are bound between subunits.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glyoxalase I family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.5" evidence="3 9"/>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78493.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322912.2">
        <property type="nucleotide sequence ID" value="XM_003322864.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2C4"/>
    <dbReference type="STRING" id="418459.E3K2C4"/>
    <dbReference type="GeneID" id="10536174"/>
    <dbReference type="KEGG" id="pgr:PGTG_04449"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04449"/>
    <dbReference type="eggNOG" id="KOG2944">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046006_1_3_1"/>
    <dbReference type="InParanoid" id="E3K2C4"/>
    <dbReference type="OrthoDB" id="16820at2759"/>
    <dbReference type="UniPathway" id="UPA00619">
        <property type="reaction ID" value="UER00675"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004462">
        <property type="term" value="F:lactoylglutathione lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07233">
        <property type="entry name" value="GlxI_Zn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.180.10">
        <property type="entry name" value="2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029068">
        <property type="entry name" value="Glyas_Bleomycin-R_OHBP_Dase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004360">
        <property type="entry name" value="Glyas_Fos-R_dOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004361">
        <property type="entry name" value="Glyoxalase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018146">
        <property type="entry name" value="Glyoxalase_1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037523">
        <property type="entry name" value="VOC_core"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00068">
        <property type="entry name" value="glyox_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10374:SF30">
        <property type="entry name" value="LACTOYLGLUTATHIONE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10374">
        <property type="entry name" value="LACTOYLGLUTATHIONE LYASE GLYOXALASE I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00903">
        <property type="entry name" value="Glyoxalase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54593">
        <property type="entry name" value="Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00934">
        <property type="entry name" value="GLYOXALASE_I_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00935">
        <property type="entry name" value="GLYOXALASE_I_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51819">
        <property type="entry name" value="VOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="VOC" evidence="10">
        <location>
            <begin position="124"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="7">
        <location>
            <position position="262"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR604361-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR604361-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP78493.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="270" mass="30493" checksum="9F85FE9BD838018E" modified="2012-04-18" version="2">MLRRFQIYGGRNIGISSLAEFHGFAVGGSRGVPTGGTMTRWGSLESLLLTSQTDITQISNSTIASRTRHRLTSKLGVRTTRLKQHYIAAMRTLLESVINLKTHLNLSANSPIRRSHIASQQTFRLNHTMFRIKDPKISLEFYQDILGMKLLHKMEVESAKFTNYFLGFPGSNQDSKSSSPLHREGVVELCHNWGTESDPDFSGYHNGNKSPQGFGHIAITCDDVEKTCAYLEEKQVKFQKRLKDGSMKEIAFIQDPDGYWIEILGNTFFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2F4</accession>
    <name>E3K2F4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">COPII-coated vesicle protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_04479</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cornichon family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78523.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322942.2">
        <property type="nucleotide sequence ID" value="XM_003322894.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2F4"/>
    <dbReference type="FunCoup" id="E3K2F4">
        <property type="interactions" value="512"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K2F4"/>
    <dbReference type="GeneID" id="10536287"/>
    <dbReference type="KEGG" id="pgr:PGTG_04479"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04479"/>
    <dbReference type="eggNOG" id="KOG2729">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_112942_0_1_1"/>
    <dbReference type="InParanoid" id="E3K2F4"/>
    <dbReference type="OrthoDB" id="434393at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030134">
        <property type="term" value="C:COPII-coated ER to Golgi transport vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005102">
        <property type="term" value="F:signaling receptor binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003377">
        <property type="entry name" value="Cornichon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033466">
        <property type="entry name" value="Cornichon_conserved"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12290">
        <property type="entry name" value="CORNICHON-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03311">
        <property type="entry name" value="Cornichon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01398">
        <property type="entry name" value="Cornichon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01340">
        <property type="entry name" value="CORNICHON"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP78523.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="137" mass="15918" checksum="E5E3C3A35C04DF74" modified="2012-04-18" version="2">MSAEAWLFLFAVVLAAVLLFTAVFYIIMFSDLECDYLNPIDLCNKMNQFVLPEMGAHMFLVLLFVLSFQLVATLINVPLVAWNVNKVMKKTHMYDATEIFRTLAQHKKESFFKLGFYLLSFFYYLFRMIAALVADEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2N5</accession>
    <name>E3K2N5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04560</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78604.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323023.2">
        <property type="nucleotide sequence ID" value="XM_003322975.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2N5"/>
    <dbReference type="GeneID" id="10536453"/>
    <dbReference type="KEGG" id="pgr:PGTG_04560"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04560"/>
    <dbReference type="HOGENOM" id="CLU_150829_1_0_1"/>
    <dbReference type="InParanoid" id="E3K2N5"/>
    <dbReference type="OrthoDB" id="10269269at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172349" evidence="1">
        <location>
            <begin position="20"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78604.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="106" mass="11406" checksum="A0FB169E4B0F94F6" modified="2012-04-18" version="2">MHCLFYLACFLAVLQSALAGPTIAPQADTTATKGHSDEKCWGVYSYSCFGGGMYSAFNSLVYPWSWYGASSYLPYNSLFSYSGYPGYGGLWKKDASDAGKTPSSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2U7</accession>
    <name>E3K2U7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_04622</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323085.1">
        <property type="nucleotide sequence ID" value="XM_003323037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2U7"/>
    <dbReference type="GeneID" id="10536519"/>
    <dbReference type="KEGG" id="pgr:PGTG_04622"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04622"/>
    <dbReference type="HOGENOM" id="CLU_011407_0_0_1"/>
    <dbReference type="InParanoid" id="E3K2U7"/>
    <dbReference type="OrthoDB" id="2506814at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041320">
        <property type="entry name" value="CxC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040521">
        <property type="entry name" value="KDZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18802">
        <property type="entry name" value="CxC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18758">
        <property type="entry name" value="KDZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxC1-like cysteine cluster associated with KDZ transposases" evidence="2">
        <location>
            <begin position="134"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP78666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="400" mass="45937" checksum="C238BAAF973A2E49" modified="2011-01-11" version="1">MPAFEGVGGRKKKRTRKPTTSTGIQLQQSLALEVVDAIRTSENISQEHITHDYQDHPSQLEIRKTYDYSTNQQETFEGQETAYDQPTDPQVDNLLAYLQTEDYKRKKLLEEKHWNEVFDGMFSCFYQCAARTSNWGDVAAWDCDWKEPCQCPRTRLRQVVLVDILTRRRTEVDFCGCHPDQVRLIQMGYIGGSPKFPQTAFSIRLLRFHHIIWKHSAVAMTPFAKAIDEFLDSNNPLILVNSNSEDSNNSFSVRQWRRPMSSAVDAYREMLRREKLLTEELLKMEPIDKLSAICPKCFGPPVPGKKENEPDFIVCMDGNFQHRRHLAASSGIPEQNKTFTLFIDPDEVLQMESTMRGNIQPDEVNDNLDRCTEQHTAANDVRGPTTWKACDDTGIFGMAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2U8</accession>
    <name>E3K2U8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_04623</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323086.1">
        <property type="nucleotide sequence ID" value="XM_003323038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2U8"/>
    <dbReference type="GeneID" id="10536520"/>
    <dbReference type="KEGG" id="pgr:PGTG_04623"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04623"/>
    <dbReference type="HOGENOM" id="CLU_909550_0_0_1"/>
    <dbReference type="InParanoid" id="E3K2U8"/>
    <dbReference type="OrthoDB" id="10040649at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP78667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="306" mass="33279" checksum="C460931830031C94" modified="2011-01-11" version="1">MVQAGQGQFRLARSVQVEVRSGWSRSVQAGQGQVCSGWSRLIQAGQGWFRLAKAGQGLFRSEVCSGWLRLVQLAEVCSGRGLFRSEVCSGWLRLVQLAEVCSGRGMFRSKVCSGWLRLVQLAEVCSGPRSRSVQVRGLFRLVEVGSAGRGLFRLRSVQAGQGLFRLAKVRSVQAGQGRFRIVEVVQADGGPFNQGWFRLVEVGSAGRGLFRSRAVQVEVCSGRGLFRLSVQVEVCSDVSPGWLRSLQVSQGWLRLVEVCSEVSSGRSMPVPRGPNKLSAFVLISHAASNAGVEVAHFNVINKTLQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3K445</accession>
    <name>E3K445_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">CBF1-interacting co-repressor CIR N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_04803</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CWC25 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78847.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323266.2">
        <property type="nucleotide sequence ID" value="XM_003323218.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K445"/>
    <dbReference type="FunCoup" id="E3K445">
        <property type="interactions" value="258"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K445"/>
    <dbReference type="GeneID" id="10536949"/>
    <dbReference type="KEGG" id="pgr:PGTG_04803"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04803"/>
    <dbReference type="HOGENOM" id="CLU_025093_0_0_1"/>
    <dbReference type="InParanoid" id="E3K445"/>
    <dbReference type="OrthoDB" id="21123at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005684">
        <property type="term" value="C:U2-type spliceosomal complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019339">
        <property type="entry name" value="CIR_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022209">
        <property type="entry name" value="CWC25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051376">
        <property type="entry name" value="CWC25_splicing_factor"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16196">
        <property type="entry name" value="CELL CYCLE CONTROL PROTEIN CWF25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16196:SF0">
        <property type="entry name" value="PRE-MRNA-SPLICING FACTOR CWC25 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10197">
        <property type="entry name" value="Cir_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12542">
        <property type="entry name" value="CWC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01083">
        <property type="entry name" value="Cir_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="CBF1-interacting co-repressor CIR N-terminal" evidence="10">
        <location>
            <begin position="11"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="112"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="162"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="21"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="130"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="181"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="206"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="251"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="272"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="297"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="324"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="335"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="362"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="379"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="441"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP78847.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="508" mass="57657" checksum="A62C8CCF032AB1D1" modified="2012-04-18" version="2">MGGGDLNMKKSWHPLLMKNQERVWKEERKALEERKKTAQLQKELAEERQLQELQRIQAEQGGGRREERVDWLYATPAIGNGINAEEQEAYLLGKKTVDKLFKEKDEAAAKLQARAASQSDADKAGGGFISLQNANTARDTASKIREDPLLAIKQQEQLAYENLKKNPARVKALQSSLTTKEQSRKEKEERKQLKRDLKEARKRRHDPAEDARGRSLEGRNESKRARGGKHHSRESDQSVSPDGHSRSYRRGSPDRRHSPDRRPHRRHSPPSPDDRRRSDSPDRTSRPSRRTSDHPSGHHHRTSTSPDHRHRGSRHTGRSRSRSPSRTSRNQSSKHTTDREPPHAHHSSNDRNYHKAPLPGRSHHDSSSRHYPDSGRPRNPSSSFQRSNQGPSYPPTSSTPADRTAEPAKSTQKLSEAKAAKLAEMQAAAKSLQESRSQRLQKLEAEDSERMKIEEAERERNRIKSGGVGDGNKAKFVMEQEKKVFFGQMGLAERVKRSGGVGLLKDKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3K559</accession>
    <name>E3K559_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05695</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323793.1">
        <property type="nucleotide sequence ID" value="XM_003323745.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K559"/>
    <dbReference type="GeneID" id="10531198"/>
    <dbReference type="KEGG" id="pgr:PGTG_05695"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05695"/>
    <dbReference type="HOGENOM" id="CLU_512034_0_0_1"/>
    <dbReference type="InParanoid" id="E3K559"/>
    <dbReference type="OrthoDB" id="2498246at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="532" mass="60581" checksum="941EBFE3F52EF6A7" modified="2011-01-11" version="1">MASQAKAKLVSGILSTSQIVNLSDLVKCVITALPEELFLSREPVNLKSDNADVFKGLDLTFLGSRQPKRISEEFDFSYNPENDPFDLFPTIGLSVSEEKGGRHAAQDQEAESYHRRDAVSNERLQVNQQIQPPISQVHAIVLPTEPHAETSEAVLEGQPDDIKPIGSNRRLKVDDQIQRSINQVREPIRTEVDSEGSEIASGAEWYGRKRVGSNNTKRLKGDQGIRQAIDQFPTLVSTETHAESPVSKAEQMRKPERNHTRKVSSQTGKRMKDTEKDHKYTARDSKYTTDYDARYFKGKDDSMLVLVRRAKEALDHQTISEATEFELFRGRVNEQFKEIAGWYKSTSPDRIFKIGGLETIIRKLSIASEDGERCCYHVNICLPLRSMSNHGLPIKLKINALLTALDLLHNLVRLNGLDRKILRTTEPNHKRLFEWVYDLIFADTPDHLPLLGKSQVKLPIKTGDKAFNEAQRFLHVYLTKPKRIGEFAASIAALDLLELWYKSQLSELEKSTFSSQEYKKFLFRAVQRYVRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3K5G3</accession>
    <name>E3K5G3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GPI-anchored wall transfer protein</fullName>
            <ecNumber evidence="8">2.3.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_05770</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">A acetyltransferase, which acetylates the inositol ring of phosphatidylinositol during biosynthesis of GPI-anchor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endoplasmic reticulum membrane</location>
            <topology evidence="8">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the PIGW family.</text>
    </comment>
    <dbReference type="EC" id="2.3.-.-" evidence="8"/>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79449.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323868.2">
        <property type="nucleotide sequence ID" value="XM_003323820.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K5G3"/>
    <dbReference type="FunCoup" id="E3K5G3">
        <property type="interactions" value="100"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K5G3"/>
    <dbReference type="GeneID" id="10531271"/>
    <dbReference type="KEGG" id="pgr:PGTG_05770"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05770"/>
    <dbReference type="eggNOG" id="KOG0411">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020802_1_0_1"/>
    <dbReference type="InParanoid" id="E3K5G3"/>
    <dbReference type="OrthoDB" id="15270at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032216">
        <property type="term" value="F:glucosaminyl-phosphatidylinositol O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009447">
        <property type="entry name" value="PIGW/GWT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20661">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS W PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20661:SF0">
        <property type="entry name" value="PHOSPHATIDYLINOSITOL-GLYCAN BIOSYNTHESIS CLASS W PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06423">
        <property type="entry name" value="GWT1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017321">
        <property type="entry name" value="GWT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="8" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 8" id="KW-0337">GPI-anchor biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="80"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="190"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="223"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="303"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="331"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="371"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="423"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="456"/>
            <end position="478"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="499"/>
            <end position="524"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="536"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="97"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="97"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU280819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP79449.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="563" mass="63184" checksum="524C9421C332FC33" modified="2012-04-18" version="2">MTSYKEEKEGFVSNGGGCSGSDIVAILASLLLTHLLHRSLNNNHVEKRPDGTTFRRMMTELTSLVVPLLAITTILSEWLWTSNLCLALAALIGSRMRRETRKEERRGPGATPSAIISSNDQSEHPLLTEHHPHHTPLDGDSQNTSLRRRLIESDHQVLLTGFPVPDPSSSSSTPQIPAVLRFSHQPFLTIYRAQLVLLTSISILAVDFTIFPRKFAKTESWGISLMDSGVGSFVFSLGIISALPILKRVSSSLCSTNLPQQPILSELWRSTRKTVPLFLLGLIRIIFVKGVDYPEHVTEYGVHWNFFFTLSILPLTGVLTKRFYERFRINLALIGLLLAICHQSLLSVVGIQEYVLSSRERTSLISANREGLASIPGYMVIYVLGLSAGTYILPPSPDFLDRVLRDRQNRPDKLVSRQPGKALVVYSSWAIIWWSLFFLCNLFGFPPSRRLANPTYCFWIAALNLSLITGHSFIHDVLFRPDHPSSKPLERSQNSTKNIPLIFQFINQNSLAIFLFANLLTGLINLSVHSIYLDPTLAFFLLLLYSVLITIFAWSIKSFKLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3K5L2</accession>
    <name>E3K5L2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Blocked early in transport 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_05790</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003323840.2"/>
    </dbReference>
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    <dbReference type="FunCoup" id="E3K5L2">
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    </dbReference>
    <dbReference type="STRING" id="418459.E3K5L2"/>
    <dbReference type="GeneID" id="10531297"/>
    <dbReference type="KEGG" id="pgr:PGTG_05790"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05790"/>
    <dbReference type="eggNOG" id="KOG3385">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_086133_1_2_1"/>
    <dbReference type="InParanoid" id="E3K5L2"/>
    <dbReference type="OMA" id="ARQGCRW"/>
    <dbReference type="OrthoDB" id="261831at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd15853">
        <property type="entry name" value="SNARE_Bet1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000057">
        <property type="entry name" value="SNARE complex subunit (Bet1), putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039899">
        <property type="entry name" value="BET1_SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12791">
        <property type="entry name" value="GOLGI SNARE BET1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="135"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="11">
        <location>
            <begin position="65"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="19"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="50"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP79469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="157" mass="17931" checksum="EC1D440F550C9B5E" modified="2011-01-11" version="1">MSKLTHRDPNRAALLSRTPSYNSSPSSNPGATTIPNYGRSYGSYDQQLPFSSSSSSSFSNHRTAEELESQNDEAIEGLSAKVRLLKEITVNIGTEVKESSNLIATLNDKFSEATGVLSGTFKKMARMPKNQYGRWWYWFLFLIVVFWIFVFTWLWRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3K5N2</accession>
    <name>E3K5N2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06106</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324204.1">
        <property type="nucleotide sequence ID" value="XM_003324156.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K5N2"/>
    <dbReference type="GeneID" id="10533310"/>
    <dbReference type="KEGG" id="pgr:PGTG_06106"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06106"/>
    <dbReference type="HOGENOM" id="CLU_141779_0_0_1"/>
    <dbReference type="InParanoid" id="E3K5N2"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="157" mass="17303" checksum="6A69FC425F819214" modified="2011-01-11" version="1">MTGLQRVSCDSAVKKDEPDKSKLQYSEEHTKCGSGKTLATHEIGDSELGEIDICSREEDIHHLGSANKTQPDIGIGHHALFRRRRRGGEKEIRDRAYPAKHASTLIAVVGTRCIIKGIHTTYQPPLARWDKGSDWFRKSVTSLGHPDLPAAGSPVEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3K5R4</accession>
    <name>E3K5R4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05806</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323904.1">
        <property type="nucleotide sequence ID" value="XM_003323856.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K5R4"/>
    <dbReference type="GeneID" id="10531313"/>
    <dbReference type="KEGG" id="pgr:PGTG_05806"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05806"/>
    <dbReference type="HOGENOM" id="CLU_1687528_0_0_1"/>
    <dbReference type="InParanoid" id="E3K5R4"/>
    <dbReference type="OrthoDB" id="10268658at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173553" description="Secreted protein" evidence="1">
        <location>
            <begin position="29"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="156" mass="17975" checksum="07162A1F43BDBE40" modified="2011-01-11" version="1">MFLTHFLHNALLVSFTVIGLFRFSPSLASTTKSSKPPVSARCGIHLLHGDPGFFNCMTDEQQEFSCADDSCEHAGYGWKDFMFKQCREYNPDNTLSQTYQDIYVDTFLAYNANLWVEAQEKKTAKSFECSWTEHGPDKRFNARRPTCHRCQLIPEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>E3K7N8</accession>
    <name>E3K7N8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="9">5.6.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_06292</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the helicase family.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.3" evidence="9"/>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80336.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324755.2">
        <property type="nucleotide sequence ID" value="XM_003324707.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K7N8"/>
    <dbReference type="STRING" id="418459.E3K7N8"/>
    <dbReference type="GeneID" id="10539344"/>
    <dbReference type="KEGG" id="pgr:PGTG_06292"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06292"/>
    <dbReference type="eggNOG" id="KOG0987">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_293034_0_0_1"/>
    <dbReference type="InParanoid" id="E3K7N8"/>
    <dbReference type="OrthoDB" id="2505546at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043596">
        <property type="term" value="C:nuclear replication fork"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071932">
        <property type="term" value="P:replication fork reversal"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18037">
        <property type="entry name" value="DEXSc_Pif1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18809">
        <property type="entry name" value="SF1_C_RecD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010285">
        <property type="entry name" value="DNA_helicase_pif1-like_DEAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049163">
        <property type="entry name" value="Pif1-like_2B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051055">
        <property type="entry name" value="PIF1_helicase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47642:SF5">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05970">
        <property type="entry name" value="PIF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21530">
        <property type="entry name" value="Pif1_2B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="7 9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0347">Helicase</keyword>
    <keyword evidence="3 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA helicase Pif1-like DEAD-box helicase" evidence="11">
        <location>
            <begin position="144"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="DNA helicase Pif1-like 2B" evidence="12">
        <location>
            <begin position="462"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="96"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="802"/>
            <end position="868"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="930"/>
            <end position="986"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="27"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="44"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="96"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="130"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="802"/>
            <end position="812"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="848"/>
            <end position="862"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="930"/>
            <end position="949"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="953"/>
            <end position="962"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="963"/>
            <end position="973"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF05970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF21530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP80336.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1224" mass="137844" checksum="D0F2CFD62D63EA99" modified="2012-04-18" version="2">MDSSQVTRSPKSSCDAGTPIQSPAKPSMTSLQRSPSKTPISAEMRARIEENRRRAQEIKAKKQQMARIQERADQEADRRAAGEKYLREFGLGSSSTKQKLDFSSTNSNLHRHPEKSNNSFELDCNFLPNHKPPPQPYSPDPRCSAEQLKVLDLVKLGHNVFFTGSAGVGKSFMLHEVVRMLKSEKLRVSVTAPTGIAALAIGGCTIHSWARVGLGHGSAHALYNKLIAQKYKSAKFERDTDTNRIWNTDVLIVDEISMLHPDLFEKISILCQAIRKIKKPFGGIQIILSGDFFQLPPVAKDDDFTCMYCGCPKFERVGSTGEIRCVKPLHKKWDDKPCGRVRKESTFCFETPTWRQLNLQVIELTQVFRQEDKHFIDMLNKIRWGTVDDEIEEIAVSRSQPLEAHEIKPTRLYARNMNVHAENAKQFGLLDSKPYEFSASDQTMGNAPYAHTFLSRLEDLQARKKLTLKIGAQVMLLCNLDIKAKLVNGSRGVVIDWVERPSHQFSSVLSQQRDPDDMKKMYWSSKQANSCLPKVLFSDGRVMLIEPFVWNIEIDASLTLCRTQLPLSLAWAITIHKSQGQTLDRLCVDLFGIFEHGQAYVALSRARSLQGLQITGWKSSRVQCHPSVKDFYKSLHEEKRSSQDEDFLDPEDFFPFSDCIEFMYEPSPSPEPSSSESVPLHQGIPAIEYLPSDERKVFQEGVTSKESADAELISSGQFKSLCLKKANLTGDFGGKTLIGSDLKPQSDSRFHIDPLLGGSSPSVSDDLLDDDQLISVAEEVERSQGDQQQRLSQLTHVDQVEQTEVKVDTSTLRRPAKDPSFPETAEIDQDLRSPTPVYENRPLAEIGTDDHQPEIKSEPLARRRDRPVPITQEIDDNQHVQAPPFTTGADTSIPEMRVIDNQPVSPIHKDPSFSLIKAIENTRRVETLLSTEGQHQPSSEVPTLDQNQLRKPLPNKKRKEKRKLSEYDRDERIGSGPVKKSSKRANTIQTKPLALKDKANDFGNCSHTHLITGPSDPAQADPVNLYIERTYHTRSMNDPTHRSGYTDSGSTAMFINHPNRSSVAVDVKPNINQAEPVVHQSDRQKKPFDESEFSKMILNDFSRRIRDSIMMLNKSLLELPSGPYAREFDSESVEEHVRMLVGEHFCNPHLVNLPAVTDTETRSRETGQNSNLEGRMIENIGPLGQENDNRTLDAQATPNPPIRIGQLVRGLSPDFHCNNFHAPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3K9J7</accession>
    <name>E3K9J7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07357</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP81105.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325524.2">
        <property type="nucleotide sequence ID" value="XM_003325476.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K9J7"/>
    <dbReference type="GeneID" id="10536427"/>
    <dbReference type="KEGG" id="pgr:PGTG_07357"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07357"/>
    <dbReference type="HOGENOM" id="CLU_030899_0_0_1"/>
    <dbReference type="InParanoid" id="E3K9J7"/>
    <dbReference type="OrthoDB" id="10305747at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="401"/>
            <end position="532"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="552"/>
            <end position="580"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="264"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="420"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="460"/>
            <end position="471"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="482"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="510"/>
            <end position="525"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81105.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="580" mass="65165" checksum="038842D63D10527E" modified="2012-04-18" version="2">MILCRPHRSHRQGRSSSQESSPQSDYHTNPSTLQYNLVFSLPIIFLSITPAKSGDPTSTKIRDCNLIMSTHSNVSTTKDSKAKQAPAAEQTSSARSITAPKRSSTKIPRNKAKKPISPEIVPSEWDTSSEEATKSDKPKPTSSTAPGLKKSSTTSDPFKSKKPLEKLIDSELLKNISIKKSVPQEASTTVAVPIVHTAEPVKLVEKQHPNASHHPVDSSTAQTVIHQTPVSSIPPSLPKISSDAAVQKKLENYFVPQGRTTRSISSSSSVLPSGSFTTEPYEPADSSDLDIITGATAQLWSKPKTSKEPQEDDILQKYRPSFHPLLQTVKLQREYYQKAIETKDEDLKIVALRAASSAQSDLLRAMNQEEFLNLFNRWDPIAEYQDYLTGQAGRNYWRKEGVAVTPSPSPEVEMRPPESVQRNQTTDSANQHQEPMNVHYPVGHPPPSANSRQYEHYRNFPNNPETTQGYQQHPVVAHHRPSSANNRSRNRNRNSNNSRNQEYRAANFRPRNSNGPRHRTNSQTARENRRIAHQRSIHQEMIRLNRTTQAQYQALEAMREQNAGYGNRRQPQEGEENQNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KA34</accession>
    <name>E3KA34_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07268</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP81016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325435.1">
        <property type="nucleotide sequence ID" value="XM_003325387.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KA34"/>
    <dbReference type="GeneID" id="10536255"/>
    <dbReference type="KEGG" id="pgr:PGTG_07268"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07268"/>
    <dbReference type="HOGENOM" id="CLU_150829_1_0_1"/>
    <dbReference type="InParanoid" id="E3KA34"/>
    <dbReference type="OrthoDB" id="2507116at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173363" evidence="1">
        <location>
            <begin position="20"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="117" mass="12633" checksum="FF58E043010C8C31" modified="2011-01-11" version="1">MNCLFYGACFLALLQSALAMPTLAPRADQVAASHKGQSDEKCYFGCGFSNYGLYGSPFGSSYLSNYMSPYFYGNSLYGGLNYNLAYNSYGSDFGIGANVGLFKKDASEANKHQENSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAA6</accession>
    <name>E3KAA6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein-serine/threonine kinase</fullName>
            <ecNumber evidence="8">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_06884</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the PDK/BCKDK protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="8"/>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81263.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325682.2">
        <property type="nucleotide sequence ID" value="XM_003325634.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KAA6"/>
    <dbReference type="STRING" id="418459.E3KAA6"/>
    <dbReference type="GeneID" id="10536750"/>
    <dbReference type="KEGG" id="pgr:PGTG_06884"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06884"/>
    <dbReference type="eggNOG" id="KOG0787">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023861_4_1_1"/>
    <dbReference type="InParanoid" id="E3KAA6"/>
    <dbReference type="OrthoDB" id="3264224at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004740">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010510">
        <property type="term" value="P:regulation of pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.20">
        <property type="entry name" value="Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036784">
        <property type="entry name" value="AK/P_DHK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018955">
        <property type="entry name" value="BCDHK/PDK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039028">
        <property type="entry name" value="BCKD/PDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947:SF20">
        <property type="entry name" value="[3-METHYL-2-OXOBUTANOATE DEHYDROGENASE [LIPOAMIDE]] KINASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10436">
        <property type="entry name" value="BCDHK_Adom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69012">
        <property type="entry name" value="alpha-ketoacid dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="7 8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Branched-chain alpha-ketoacid dehydrogenase kinase/Pyruvate dehydrogenase kinase N-terminal" evidence="10">
        <location>
            <begin position="18"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase/HSP90-like ATPase" evidence="9">
        <location>
            <begin position="289"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF10436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP81263.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="395" mass="43787" checksum="861D5B942603270E" modified="2012-04-18" version="2">MASSTHHDHSLTGQSLKKVSLDRLMRFGTPPLSPSKLIESAELTRQELIQRIQRRVNAHLSLPYLPASNPHIKQVMSIYRRSFEEINSLPPIRTVEDNAALLQALVTMVDDATDVIGMFATGFKESKRYLSEEQISSFLNRAIQSRISIRLIAEQHLSLSKAEHSPSPSRTGIVDKKMNLKKTLESVLQFAAELCEGTFGIAPEWRLSGEVEAEVCFVEMHVSWYRVNVFSTLLSALRATVEHHREVTKTTNPPLPPIEVAVAVERPPRVTVNSKETTDRSLSDAEISKSILCIRIRDHGGGIDPQDLPRVFSYAFSTVGSEDNAKDGMHNQADFDRISYGQEELKSSLGRIAGLGFGLPMARLYCRYFGGNLELVNMHGVGGGVDSYITIGVGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAM1</accession>
    <name>E3KAM1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Saccharopine dehydrogenase [NADP(+), L-glutamate-forming]</fullName>
            <ecNumber evidence="10">1.5.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Saccharopine reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGTG_06954</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-saccharopine + NADP(+) + H2O = (S)-2-amino-6-oxohexanoate + L-glutamate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10020"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57951"/>
            <dbReference type="ChEBI" id="CHEBI:58321"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.5.1.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (fungal route): step 2/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the saccharopine dehydrogenase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 14">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <dbReference type="EC" id="1.5.1.10" evidence="10"/>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325752.1">
        <property type="nucleotide sequence ID" value="XM_003325704.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KAM1"/>
    <dbReference type="FunCoup" id="E3KAM1">
        <property type="interactions" value="323"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KAM1"/>
    <dbReference type="GeneID" id="10536905"/>
    <dbReference type="KEGG" id="pgr:PGTG_06954"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06954"/>
    <dbReference type="eggNOG" id="KOG0172">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1562">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016207_0_1_1"/>
    <dbReference type="InParanoid" id="E3KAM1"/>
    <dbReference type="OMA" id="ECLWIHL"/>
    <dbReference type="OrthoDB" id="10059875at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004755">
        <property type="term" value="F:saccharopine dehydrogenase (NADP+, L-glutamate-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004753">
        <property type="term" value="F:saccharopine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019878">
        <property type="term" value="P:lysine biosynthetic process via aminoadipic acid"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015940">
        <property type="term" value="P:pantothenate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006596">
        <property type="term" value="P:polyamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000008">
        <property type="entry name" value="Alpha-aminoadipic semialdehyde synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000072">
        <property type="entry name" value="Saccharopine dehydrogenase [NADP(+), L-glutamate-forming]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1870.10:FF:000002">
        <property type="entry name" value="Saccharopine dehydrogenase Lys9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.140.10:FF:000001">
        <property type="entry name" value="SPE3p Spermidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000013">
        <property type="entry name" value="Spermidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1870.10">
        <property type="entry name" value="Domain 3, Saccharopine reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.140.10">
        <property type="entry name" value="Spermidine synthase, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00198">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051168">
        <property type="entry name" value="AASS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030373">
        <property type="entry name" value="PABS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032095">
        <property type="entry name" value="Sacchrp_dh-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001045">
        <property type="entry name" value="Spermi_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035246">
        <property type="entry name" value="Spermidine_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037163">
        <property type="entry name" value="Spermidine_synt_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002010">
        <property type="entry name" value="PRK00811.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00417">
        <property type="entry name" value="speE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133:SF22">
        <property type="entry name" value="ALPHA-AMINOADIPIC SEMIALDEHYDE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11133">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16653">
        <property type="entry name" value="Sacchrp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17284">
        <property type="entry name" value="Spermine_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01564">
        <property type="entry name" value="Spermine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01330">
        <property type="entry name" value="PABS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 13 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="15">
        <location>
            <begin position="12"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00066976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00083134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EFP81333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="759" mass="83822" checksum="E0ADFA51499B4451" modified="2011-01-11" version="1">MATTLTHPAIKDGWFREESNLWPGQAMSLQVKKVLHVEKSKFQDVLVFESTSHGNVLVLDGAIQCVENDEFSYQEMIAHLPLNSHPNPEHVLVIGGGDGGVLREVLKHPSVKTATLVDIDEAVPRVSKTFLPHMAAGFNDPRVTVHIGDGFQYLRDQVGKFDVIITDSSDPNEGPAQTLFGMPYFELLKNALKPNGSIATQGECIWLHLPLIHSLIKGAKDLFPQVEYAYTSIPTYPSGTIGFVVCSLDKDRKLKQPLRQVRNTKYYNKNVHAAAFVLPEFARQAVEAAKANLDMPDKSASAQSSVPGKKILLLGSGFVAQPAADYLLRRPENQVTVASFNLWKAERFATELAREVKCISLDINNSEALDKAVSEHDLVISLVPYIHHASVIKSAIKFKKNVVTTSYVSPAMRALDEEVKKAGITVLNEIGVDPGVDHLYAVKMIDTVHRAGGKIIEFISYCCGLPAPECSNNPLGYKFSWSSRGVLLALLNSAKLYSKGKLIEIEGQELMNHAQPYFISPAFAFVAYPNRDSTPFREFYEIPEAQTVVRGTIRYQGFPAFIKTLVDIGFLNETPQAYLRPESTLPWKEVTARILGADDSTERCLVSEIKKRTTFPNSDEEVRILAGLKWIGIFSDDHAVPRGNILDTLCARLETKMQYEKDERDMVVLQHKFGIQWQDGSKETRTSTLVEYGAPFQSGTGPSAMAKLVGVPCGIAVQLILDGKITKKGVLAPYTLDIVAPLLAEVEKEGISMVDQIVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAS2</accession>
    <name>E3KAS2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06812</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325610.1">
        <property type="nucleotide sequence ID" value="XM_003325562.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KAS2"/>
    <dbReference type="GeneID" id="10536590"/>
    <dbReference type="KEGG" id="pgr:PGTG_06812"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06812"/>
    <dbReference type="HOGENOM" id="CLU_1644553_0_0_1"/>
    <dbReference type="InParanoid" id="E3KAS2"/>
    <dbReference type="OrthoDB" id="2506495at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042720">
        <property type="term" value="C:mitochondrial inner membrane peptidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024645">
        <property type="entry name" value="Mitochondr_Som1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11093">
        <property type="entry name" value="Mitochondr_Som1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="161" mass="17937" checksum="914291ECBF5AC879" modified="2011-01-11" version="1">MARMQFGGRPVEEDDEAGLLRLNNYKRNEPRKQMGTLHTTMSTTPVVRGPAGPAEPPPRPAAVLPIVEPVRITEGVEELVGDGCRGLISRLEQFSCRPDSLRGTIQNPQWICVPIHRFFKLCRSASRTISVEIPPPQSTDPTRPTKKNELTSNERIEEALT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3KB28</accession>
    <name>E3KB28_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_07809</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325979.1">
        <property type="nucleotide sequence ID" value="XM_003325931.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KB28"/>
    <dbReference type="STRING" id="418459.E3KB28"/>
    <dbReference type="GeneID" id="10532941"/>
    <dbReference type="KEGG" id="pgr:PGTG_07809"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07809"/>
    <dbReference type="HOGENOM" id="CLU_376481_0_0_1"/>
    <dbReference type="InParanoid" id="E3KB28"/>
    <dbReference type="OMA" id="RVGGHGM"/>
    <dbReference type="OrthoDB" id="2507858at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173395" evidence="2">
        <location>
            <begin position="23"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="169"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="210"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="228"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="303"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="323"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="357"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="420"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="442"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="459"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="558"/>
            <end position="567"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP81560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="592" mass="57232" checksum="260C883D78C98404" modified="2011-01-11" version="1">MPRTSLICLVALAARQGYFANAAAIAATASSSSSASASAGSTYMVNGQVVKSSSSSAQAGAGAYAYADSDGHQLEKHWSNSSTNGSKKKCDCEDKGSKPCDQQKQDNGNSPVPGVSSGDTSPSGSNQPGSNYPTDASSGSGNSYPSTPSGSAYPSQQGSNCTGGGAGSSSGCTSASSYPDSSPSPNSSGYPSVSSGGSDYPSGPSGGNNYPSVSSGGSDYPTGSSGDNNYPSVSSGGSNYPSSPSVDNNYPSVSSGGSNYPSGPSGGNNNYPSITSGGSGYPSSNTTSGPSPSGNGYPLPSNDYPSTSGNNTPPISSGGGSYPSGSSGTSPQGNSYPSAPSGGSDYPSVTSGGNGYPTNSTGSNSYPSGSPSVTSGGGDVPPPVSSGTNDYPSGPAGNDSPYPSTSGNTGCGCEKGSGDSYPSTPSVTSGGSTPGGIPYPGTPSVTSGGSSPGGNNYPDTPSVTSGDSYPGGQPQNTPPVSSGGSYPSIQSPSGSPKNSSYPSNGNVPTPSGNNSTSPTHPCSTSDTSGGSNCDTPTPSNKSPDGSSPKPHDSGYPDNSTTTNPNAATNLVGHSTIEILLLTLASGFAVTFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KC73</accession>
    <name>E3KC73_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_08089</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EFP81840.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326259.2">
        <property type="nucleotide sequence ID" value="XM_003326211.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KC73"/>
    <dbReference type="GeneID" id="10533876"/>
    <dbReference type="KEGG" id="pgr:PGTG_08089"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08089"/>
    <dbReference type="HOGENOM" id="CLU_059063_0_0_1"/>
    <dbReference type="InParanoid" id="E3KC73"/>
    <dbReference type="OrthoDB" id="10323926at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="373"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP81840.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="393" mass="44096" checksum="95989E46343E8D01" modified="2012-04-18" version="2">MSFWHLRFGDLAASLSVRQRPSALTQKVVLGSLPWELRGQIIANVVKTDPTSLNSSKLQISRSLGAGSSTMQLCIWHLGRFLKCLDEPDLSEKYKLSNLPTSSMTGHNFGTLKTKGCLELSFLEQLFCTRTQPYDLVNGLNEADNLSDSEAEATVSSAYSPLPRSGKVPVGFQGFYDEDDSIFQRQNKNLSPDGTANRVMGQTSRSSFEGKTKTQSDIEDMSNEASSHHNLAQYHDGRPAVSDPVQLLLIVTALTLSSKWSNLKETARSYGIGSEETSSSNGPTYLPRYYNHEAHSYSGDEFEKENELKDAASKNIDYARGYGKGYDSSEEAHREVSPINYDSDEYHSRRFIGYQLTLAEKCLPRNQKPPSDLLWILVLAFVLKMWSWYYYSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KD49</accession>
    <name>E3KD49_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_07492</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326514.1">
        <property type="nucleotide sequence ID" value="XM_003326466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KD49"/>
    <dbReference type="GeneID" id="10534626"/>
    <dbReference type="KEGG" id="pgr:PGTG_07492"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07492"/>
    <dbReference type="HOGENOM" id="CLU_2134785_0_0_1"/>
    <dbReference type="InParanoid" id="E3KD49"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP82095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="113" mass="12391" checksum="AFC467341AD93126" modified="2011-01-11" version="1">MSSTHFLRLPLPSITKQIFSRRSPSSKFNLSPFLTCNQPSHWPSAFTSNLHLQRSPSISFTGNARPSNSSLSSNPELVLSPPIISKHLIFIAGPVFTIVDTLSFLTSHVTSPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDF6</accession>
    <name>E3KDF6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08348</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326811.1">
        <property type="nucleotide sequence ID" value="XM_003326763.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDF6"/>
    <dbReference type="GeneID" id="10529769"/>
    <dbReference type="KEGG" id="pgr:PGTG_08348"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08348"/>
    <dbReference type="HOGENOM" id="CLU_1907707_0_0_1"/>
    <dbReference type="InParanoid" id="E3KDF6"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173753" description="C2H2-type domain-containing protein" evidence="1">
        <location>
            <begin position="28"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="133" mass="14860" checksum="2B1E4A8E11E05E97" modified="2011-01-11" version="1">MKLETLTLVSVLIGLFCAVLQCGQVQAISECPIPNCGRCGQAMSLMTHNQQCRKAFLCKTHGRPTGRQCTVMREGSYWRCHACDEMLDGLFHRRCLRTHLTPHGGICVSVTDHRALQLARQESLCYGPVVRTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDZ1</accession>
    <name>E3KDZ1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">OBG-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_08309</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82353.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326772.2">
        <property type="nucleotide sequence ID" value="XM_003326724.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDZ1"/>
    <dbReference type="FunCoup" id="E3KDZ1">
        <property type="interactions" value="993"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KDZ1"/>
    <dbReference type="GeneID" id="10529728"/>
    <dbReference type="KEGG" id="pgr:PGTG_08309"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08309"/>
    <dbReference type="eggNOG" id="KOG1487">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_044997_0_0_1"/>
    <dbReference type="InParanoid" id="E3KDZ1"/>
    <dbReference type="OrthoDB" id="603at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005777">
        <property type="term" value="C:peroxisome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903833">
        <property type="term" value="P:positive regulation of cellular response to amino acid starvation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd01896">
        <property type="entry name" value="DRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1070">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045001">
        <property type="entry name" value="DRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031662">
        <property type="entry name" value="GTP-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43127">
        <property type="entry name" value="DEVELOPMENTALLY-REGULATED GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16897">
        <property type="entry name" value="MMR_HSR1_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="3">
        <location>
            <begin position="102"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="4">
        <location>
            <begin position="271"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP82353.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="315" mass="34585" checksum="162405D11711F121" modified="2012-04-18" version="2">MSTAVEKIKAIEDEMAKTQKNKATAFHLGQLKAKLAKLKRELLTPSSSGGGGPGVGFDVARTGVASVGFVGFPSVGKSSLMSGLTGTQSAVAAYEFTTVVSIIEGAKDGKGRGRQVIAVARSCNLLFIVLDVLKPLDDKRIIENELEGFGIRLNKKPPNIIIKKKDKGGISISNTVPLTKLEPEEIKAVLAEYRMSNCDVAFRCDATLDELIDVIEGNRVYMPCLYVLNKIDAISIEELDLLYRIPNSVPISVKEWLNIDELIDALWYKLDLVRVYTKPRGRQPDYSQPVVLKTAKCTVEDFCNAIHKEIAKQMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3KH50</accession>
    <name>E3KH50_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_09338</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328044.1">
        <property type="nucleotide sequence ID" value="XM_003327996.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KH50"/>
    <dbReference type="GeneID" id="10532311"/>
    <dbReference type="KEGG" id="pgr:PGTG_09338"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09338"/>
    <dbReference type="HOGENOM" id="CLU_054434_0_1_1"/>
    <dbReference type="InParanoid" id="E3KH50"/>
    <dbReference type="OMA" id="NTWNGRY"/>
    <dbReference type="OrthoDB" id="2509924at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47481:SF7">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="237"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="60"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP83625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="287" mass="31467" checksum="52AA9C1B463F59F4" modified="2011-01-11" version="1">MSTRQVQNVASLVSTVKALDGTAAVFPVWRSRIEDVLSMQNTLDIVNGSLPRPKEDSKSDSSVARSTDYSKGYNPKEVVADWDALSELACATIRLTLSRPLALRYRKTKPATRLFTTIVNAYEKNTRARRIRLQEAFWESKHDPNTPIALWIGHVRVAADELLTINELPTDRQIADRLVAGLDSSWSAVKDSIVYTAQEMSLDDTIGALEAHEVSLNGKTSTDFASASFASSKRFGCSNCGKRGHKSSECRKPKTKAGAVSTVKLGGYDSGSFDDGDEIDVVKNLYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHJ8</accession>
    <name>E3KHJ8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09486</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83773.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328192.2">
        <property type="nucleotide sequence ID" value="XM_003328144.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHJ8"/>
    <dbReference type="GeneID" id="10532460"/>
    <dbReference type="KEGG" id="pgr:PGTG_09486"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09486"/>
    <dbReference type="HOGENOM" id="CLU_479075_0_0_1"/>
    <dbReference type="InParanoid" id="E3KHJ8"/>
    <dbReference type="OrthoDB" id="2507615at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172625" evidence="1">
        <location>
            <begin position="20"/>
            <end position="586"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83773.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="586" mass="66896" checksum="57208A271FED5C97" modified="2012-04-18" version="2">MKTKTSLWILVTKVAICKADLDLDLVNLDFYFDNYKSDGYQPRLGSTTSSAQPISLHSTSSHSEFSFASATNEPTRDRTACQWMLEPSVIEERVHRPLANPQVTGLATTEPMLIDRPVRSHGDLALNSLVAGAPTTAASGNRAPDKPVSRVTSTASHWVEITPVTANEVSRKRLSMGTPLDTLFRLDDGSRKRMKMPTDLAQKVQERPSIEEIRASHWVEITPVATNEVSRKRLSMGSPLDTIFEVDGGSRKRMKMPTDQAQKVQERPSIEEIRHKASVGGQGEYVGTSRKDMMQTIGQQNAQISEPQAIDKIRVSEVEVEGLNLVFNMTVLGQILKDQSPKFFGVPWMFEKLIKSESNDSDEVLVIKPGEHAIFHEAFKEGWRLAYIKPDQESSTRDLSATPSTLAAAKLTEINKNLMVWRWLYRRRLGIDFEVLLDRIRSIIATNPKPPGFFDDMPIKMFEFFFRYLFFVDMIITILPQPTETVLKRHETFQTAVDTFEAYTKDLLKKKIFETDKIGKVAFIWKFIANWMAKDVNYSRAKMLSRTKTINVYEVNRDWKIFFSLVFSHSIIHFNRKFSKIFHNRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHS5</accession>
    <name>E3KHS5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF7918 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_09563</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EFP83850.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328269.2">
        <property type="nucleotide sequence ID" value="XM_003328221.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHS5"/>
    <dbReference type="STRING" id="418459.E3KHS5"/>
    <dbReference type="GeneID" id="10532538"/>
    <dbReference type="KEGG" id="pgr:PGTG_09563"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09563"/>
    <dbReference type="HOGENOM" id="CLU_020493_0_0_1"/>
    <dbReference type="InParanoid" id="E3KHS5"/>
    <dbReference type="OrthoDB" id="10297915at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057678">
        <property type="entry name" value="DUF7918"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36223">
        <property type="entry name" value="BETA-LACTAMASE-TYPE TRANSPEPTIDASE FOLD DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36223:SF5">
        <property type="entry name" value="BETA-LACTAMASE-TYPE TRANSPEPTIDASE FOLD DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25534">
        <property type="entry name" value="DUF7918"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7918" evidence="2">
        <location>
            <begin position="4"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="164"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP83850.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="575" mass="64308" checksum="F60D3D73E339E0E9" modified="2012-04-18" version="2">MRVIESIGTIQIDITRCSLVLEPRSRYDEDLRTSNQMKFSEQRLKASLATTAGLAPEGRYSSDSPPSMEWYDKERDPNPFLQFIFKYKPRAILENEGTIPPRVGAEVLEPDPVIVQSETECKQGIDRNRKRLKGEEMEQEEEKPQKKKPKIVADLTGSDSEDESSVKIDDEKNGSDSDAESRTLLGSPATGSSEASRRTPATGRRPISPPQPGPRPEAYRTPRTAREAVHALSNSLHDPLSSSQQRIIGDLLDRVYARFKPNNIGTEQSDFLLPVEGRDELAGVFAKHRNSHVSDSLLAEVAPKLEEVMNGMLRIPSAPARSGRIAMDDDAVSIKDWTASVESVCDSIFSPTNLRPFFPKAIDLTALLTKPDAVLSEEAHRRFRAITLSLIHPQQTQKNTWARAVNEAVFLGTNPRLPTPALNLLLQKGHYACMRFRAFLHFRNSSNSNRNQILGSLKPSKFPHQILIGFLLLGVRGLLGAPMSNRTTFHPGVCLMSDLMGLRESAPPEPIWWHTRQLIIRTINENDEHLDTRHLAAAIRQDFLAFCSINIPVIPFVLLSNQVQQLPSEVLPLEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIC9</accession>
    <name>E3KIC9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09767</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP84054.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003328425.2"/>
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    <dbReference type="AlphaFoldDB" id="E3KIC9"/>
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    <dbReference type="KEGG" id="pgr:PGTG_09767"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09767"/>
    <dbReference type="eggNOG" id="ENOG502S04P">
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    <dbReference type="HOGENOM" id="CLU_047150_0_0_1"/>
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    <dbReference type="OrthoDB" id="2588793at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84054.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="557" mass="63740" checksum="0C8DFBC23B612E63" modified="2012-04-18" version="2">MTNQTNNNHLNSFEANLYQPGSTSSSSTSNSASASASNRIHPYQSFSLSSSSNNNNNNTHPSLYIKKPFTYLNSFISTSRKPSHSIQIAILILISLSTLTILIDYWTQSQLYRLRYPWTLSPSKGSSPSTSSWKSLSEGYLGLPACDPFGEPGTVVLNTSFYHDASWVPFGPSCAPSPDYLSALRAIREVPINLPLSQRDALIHRPIHTTSSSKEQEEPQQTEFFDRSAFDLWGRPYPDLTFLRGKTILLLGDSVDRNSLEHLHQLVHADVRSLHYLDINNPPPSSDWDPRSTPWEVNLGILHPRNYSNASYPDNSRDFAGLNCKLLNGFFYGLDDIDEFSVQADWHGPGLAESRVRELYEPLTGAYGREDGEGPAFIMLQSGLWDLAFFGRRNRQRNETTELPLGAEELDWWQGRFRSLIRTIKYTWPDTPLWIRTTHRIGEQFWAAHDWQAGLKHGLGKGFVNFFPDHRVHQIRQMQLFVAKEEGLPVFDFYNLWEGYQKFQDKVHPLKVPGGVLMNQALFHHVWMESIGRKNWDPSYLTRNRIGKLPHHLHEFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIK2</accession>
    <name>E3KIK2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10505</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328546.1">
        <property type="nucleotide sequence ID" value="XM_003328498.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIK2"/>
    <dbReference type="FunCoup" id="E3KIK2">
        <property type="interactions" value="843"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KIK2"/>
    <dbReference type="GeneID" id="10545305"/>
    <dbReference type="KEGG" id="pgr:PGTG_10505"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10505"/>
    <dbReference type="eggNOG" id="KOG3117">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031901_3_0_1"/>
    <dbReference type="InParanoid" id="E3KIK2"/>
    <dbReference type="OMA" id="RHTKSER"/>
    <dbReference type="OrthoDB" id="203440at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007146">
        <property type="entry name" value="Sas10/Utp3/C1D"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237:SF9">
        <property type="entry name" value="NEUROGUIDIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13237">
        <property type="entry name" value="SOMETHING ABOUT SILENCING PROTEIN 10-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04000">
        <property type="entry name" value="Sas10_Utp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="152"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="325"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="364"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="375" mass="41516" checksum="DE7B5C1D1146BEB9" modified="2011-01-11" version="1">MESEKAVSSSLDPLGQISKASSQLSSQLAELLSSQTSELVVDGGSRSTGGGISLLELKNHLLTSYLHNLTNLLIIRLSQTSHPSTLAFEKKELGEWSSSIVQQLVWLRLVFERLRPLEARLKYQIDKLLKTVLELDQHSFSNTAAEIDHVMNDPLSFKPNPAALEAPTDTQQDREGAQEESTSGAYEQQKEDRRSKEVYRPPRVAPVAYPEAPTKTKRIAPAPVALREHVSLATMAPLPESTTGLSNTAAKSSNRAKALARMNEYEEANMTRLFSTKKDAKRRRNDEAAVALGIETSAASKNHKRKLGALEGEFDGVLSEISRKSDRYSDHLGSSSKPHNKNKNSDHQFGSNRNNNNQKFKRKGAFDKALKKISK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIU6</accession>
    <name>E3KIU6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10599</name>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84221.2"/>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003328592.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIU6"/>
    <dbReference type="GeneID" id="10545481"/>
    <dbReference type="KEGG" id="pgr:PGTG_10599"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10599"/>
    <dbReference type="HOGENOM" id="CLU_1294973_0_0_1"/>
    <dbReference type="InParanoid" id="E3KIU6"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="146"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84221.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="213" mass="23426" checksum="F2B6779651AA6378" modified="2012-04-18" version="2">MTKMGSNHPQIRGPEGLRSAIHAVYAGDGFGLSRVYYDCRGWRRQQDEAVLYAIELSKSGTIAQHPSSSYPTTTAGNGSLDAVSASGDAMYSGAFCLLVICYWLRRYQIVSRLPASFCLGWDPSTTYRSPMSSYFEIPERLVGQPLSPYSLASDDSTMPDEETTAPLESNPAIKVEEAESPAPDSPVDTRRRVEDHVVELLKLMSEELGGDKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJ56</accession>
    <name>E3KJ56_PUCGT</name>
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        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178290">
        <property type="protein sequence ID" value="EFP84331.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328750.2">
        <property type="nucleotide sequence ID" value="XM_003328702.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJ56"/>
    <dbReference type="GeneID" id="10545677"/>
    <dbReference type="KEGG" id="pgr:PGTG_10051"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10051"/>
    <dbReference type="HOGENOM" id="CLU_2134787_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJ56"/>
    <dbReference type="OrthoDB" id="10276614at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173438" description="Secreted protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84331.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="115" mass="12823" checksum="3913D668C69E6233" modified="2012-04-18" version="2">MKFLVLVTTTLLPLIPGVVYGVDFDGWCASHYGNVGKPWAICGKAEYVNNDPKQGISRWSVRPQDKCKLLKDDPTAIPFCCANSIHKIVTTRFWGWRSGIAVDAVMIKEDCHKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJQ1</accession>
    <name>E3KJQ1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Golgi to ER traffic-protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10685</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84526.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328945.2">
        <property type="nucleotide sequence ID" value="XM_003328897.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJQ1"/>
    <dbReference type="GeneID" id="10546115"/>
    <dbReference type="KEGG" id="pgr:PGTG_10685"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10685"/>
    <dbReference type="HOGENOM" id="CLU_343583_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJQ1"/>
    <dbReference type="OrthoDB" id="2509730at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053094">
        <property type="entry name" value="Zinc_metalloprotease_ZmpB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193:SF2">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="307"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="922"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="353"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="371"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="924"/>
            <end position="943"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="944"/>
            <end position="953"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="956"/>
            <end position="965"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84526.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="965" mass="103664" checksum="48D99B10E6E9B206" modified="2012-04-18" version="2">MAPVTRRTASAISGNASGEENPQVLPGAENVVDKNPADGSQGSAADSPATDSGDNINQGVNESLSGPPGASGGDKVLWKDQSGNLLVDTAAKDNAGENKDTESSGLSSSSDENPTDESGEALLKTPAPSVTPGPAPPNTPIDLDDGVGAGDQANALKDKEVPKNVAGHVAGGGTDVSTNGAGASALGTGESGNMGSTIPTLKPETPLDEVKDLHKSYSLTKAAIEASIASHVSETSPLLKSFAETFLKGLNAFLDECSSRIAKAETEGISLIEMVDLTLDDTDIEKIKAPKRKRTGLDSLKFNKFPRLNTAADGPSDRHMSDSEPEGLFGSRSPTPLPAGQAPKKNLARVESAVSQSSTAAHGAQSQTSTGPPGSQAPHPHSSQSQSAPAPSQSQPPASQATTVETSQDGEKTWKKVPERFTDPLSYQEFIVEKNQFVLALDISAMKHTISNLKKNVRDISENIHGHIAKIIGHNVKFYLEHGGYKVGSFKLKTTSPELVALCSMACTKEFQSVIRACPRLIQLEGNDPFYQSGVFNFKTIKELRTFAEAKETEGSWKALLTMMCRSADTTSETYFKDEHGIKASVNRVHALVERCMDPLQSHVEPTIDSTSPHGGFEQALQFIAKKCEILGVGANSTSGSKGNGLQFLQKRIWETLMGVLLMNYMNYREVQTKKIAQDANIAAVKGKGIVKKDIELKDLYKNIGLTMTTAQWGKMRQGALGAASIFLAFGPVGWFGCFADTNKFNLKSVYGMINLARHKSDWITKSSPASPAPPEDYKSSSAWSNISALTYSCLESFEYHLDESERTEYLDWSTLIKVWNDKLTNEVVSQAALIDVASEIASPGKSLSWEGVPAKDVIIRQGLVSESFRLEWEDGLRLGMDLPAIVRPEGATSKKAFKKKRVRNVTEKELEKARQALLKEIDQESLADDEEQDDGRDDEDGGAEERARKKGESSFILNSVGVSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3KKB2</accession>
    <name>E3KKB2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10896</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84737.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329156.2">
        <property type="nucleotide sequence ID" value="XM_003329108.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KKB2"/>
    <dbReference type="STRING" id="418459.E3KKB2"/>
    <dbReference type="GeneID" id="10526705"/>
    <dbReference type="KEGG" id="pgr:PGTG_10896"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10896"/>
    <dbReference type="HOGENOM" id="CLU_2543692_0_0_1"/>
    <dbReference type="InParanoid" id="E3KKB2"/>
    <dbReference type="OrthoDB" id="2502666at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023:SF4">
        <property type="entry name" value="GLYCINE-RICH PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84737.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="83" mass="9676" checksum="5DEEDDABF7809A78" modified="2012-04-18" version="2">MATPTTPTTPVEETTDTTQTKKKRGPNWLPRKEEQLAISWINVSKQPEFATNQLGETFYRKIEKDFNIHSKAHYCDHGQIKTW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLN4</accession>
    <name>E3KLN4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_11402</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178294">
        <property type="protein sequence ID" value="EFP85233.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329652.2">
        <property type="nucleotide sequence ID" value="XM_003329604.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLN4"/>
    <dbReference type="GeneID" id="10527222"/>
    <dbReference type="KEGG" id="pgr:PGTG_11402"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11402"/>
    <dbReference type="HOGENOM" id="CLU_104742_0_0_1"/>
    <dbReference type="InParanoid" id="E3KLN4"/>
    <dbReference type="OrthoDB" id="1637350at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173508" evidence="2">
        <location>
            <begin position="25"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP85233.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="231" mass="24328" checksum="716C4E1F0A8E7B5B" modified="2012-04-18" version="2">MSGSLALLLLGLQVLSIMLVNVAATSTNTSTLDHKLYGRSPQASAANFKPPGNNRKMPSSPGPPKDGMEDGNGTPRNPQSKGKPVPFGFGSKVTGGGNAAPQTPKDTAELKAWLTDKVPRVILIRRTYDFSLGAGNITAAACRPWKACTNGLQVQTAKDFNNWCSKDQKLPSNIQVGTDLKSQVTTRTPSMIDDRYSAQVSLQASPMDPIKVASHKTLLGVGAAGIIKGKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>E3KN36</accession>
    <name>E3KN36_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_11001</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EFP85672.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330091.2">
        <property type="nucleotide sequence ID" value="XM_003330043.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KN36"/>
    <dbReference type="FunCoup" id="E3KN36">
        <property type="interactions" value="98"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KN36"/>
    <dbReference type="GeneID" id="10544238"/>
    <dbReference type="KEGG" id="pgr:PGTG_11001"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11001"/>
    <dbReference type="eggNOG" id="KOG3565">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015390_1_0_1"/>
    <dbReference type="InParanoid" id="E3KN36"/>
    <dbReference type="OrthoDB" id="8783038at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030864">
        <property type="term" value="C:cortical actin cytoskeleton"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030036">
        <property type="term" value="P:actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd20824">
        <property type="entry name" value="C1_SpBZZ1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11912">
        <property type="entry name" value="SH3_Bzz1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.40:FF:000296">
        <property type="entry name" value="Actin polymerization protein Bzz1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000157">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035459">
        <property type="entry name" value="Bzz1_SH3_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046349">
        <property type="entry name" value="C1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020454">
        <property type="entry name" value="DAG/PE-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002219">
        <property type="entry name" value="PKC_DAG/PE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735">
        <property type="entry name" value="FCH AND DOUBLE SH3 DOMAINS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15735:SF21">
        <property type="entry name" value="PROTEIN NERVOUS WRECK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00130">
        <property type="entry name" value="C1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14604">
        <property type="entry name" value="SH3_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00008">
        <property type="entry name" value="DAGPEDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00109">
        <property type="entry name" value="C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57889">
        <property type="entry name" value="Cysteine-rich domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50002">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00479">
        <property type="entry name" value="ZF_DAG_PE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50081">
        <property type="entry name" value="ZF_DAG_PE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0728">SH3 domain</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="F-BAR" evidence="9">
        <location>
            <begin position="7"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Phorbol-ester/DAG-type" evidence="8">
        <location>
            <begin position="408"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="7">
        <location>
            <begin position="544"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="domain" description="SH3" evidence="7">
        <location>
            <begin position="627"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="484"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="484"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP85672.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="687" mass="75656" checksum="FD098219B77B1743" modified="2012-04-18" version="1">MALAQPPSFGRDLPDQLNRIQNVVHDQIAFYGDVRDYFKERVALERQYGASLQLLVRKAMEKRTKREEALSVGLETSKSWNGTSSTLDTAWSKILSEADEEASDHTNLAESLQNEVCEVLKNCEKKKEATRKRHVEFGVKLLAERDKIYHDRAKAKQRYDEACSLVESTRVKQGQAKDDRHVEKAAKNMDVHTNDMLSAKNAYLLSISVANEVKERFYHVDLPSLEDDFQSIWSLAASKLVSLLKTVSTLNLRCLDQLRTHNENFLAATNTIDAQSNQALYIEYNRRPFTDPPDFVFEPCPIWHDSAEYALSAPEPKVLLQNRLGQARSKAADLEPTIAAKRHEITGLENLREAYSQNDSLGDTDEVVENLLESVRQTITLELQYTVLTKEVEVLEQTLGDDQGSQRPHTFKSASFVTPTSCCLCNSNIWGITKQGVTCKACSISAHVKCGPKVPSNCAGTAPLHGLRRNASLAALSTSVTRQQATVDSVSREPQNQTGLSRAVSTSHRPSAAPNGGEGLARSTTTVNKMAAPLSSRNALNRTEPKPKARMIYSYEASTGFEVSALESEIVTVISPDDGSGWIKVETSSNQIGLVPATYVQIISTNAVIPPSSAPPEPATGPTPVPSRLRRAKALYDWAAQAPDEHSLSVGETVTLSKGGEAYGEGWFEIIKDGRKGIVPSNYVELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>E3KN40</accession>
    <name>E3KN40_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL23m</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_11005</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EFP85676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330095.1">
        <property type="nucleotide sequence ID" value="XM_003330047.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KN40"/>
    <dbReference type="STRING" id="418459.E3KN40"/>
    <dbReference type="GeneID" id="10544242"/>
    <dbReference type="KEGG" id="pgr:PGTG_11005"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11005"/>
    <dbReference type="HOGENOM" id="CLU_083691_0_0_1"/>
    <dbReference type="InParanoid" id="E3KN40"/>
    <dbReference type="OMA" id="ITTICHQ"/>
    <dbReference type="OrthoDB" id="275582at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032543">
        <property type="term" value="P:mitochondrial translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001159">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_uL23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12059:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL23M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12059">
        <property type="entry name" value="RIBOSOMAL PROTEIN L23-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00039977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP85676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="291" mass="33503" checksum="F0A09D0292EB965D" modified="2011-01-11" version="1">MRPAIHSNLRTIKSICHQRASIPSLRTLATTVSIDPLDRIEGEEPKPKPNQDIRECFNKLPHRPKRLDHKLIKPDQSSSDDILIRSLDKGRSPIGSSYAKAQSPVYFPNISIRLVRPSSTDRGDPFTAIFRCDLQLTKPDIFNYLRQIYGLGITSIRTAIYRGRYERQIKSKVRGGVKIAKDKNRTFKKVWVGLDKPFFYPPQPNQRFLNENYNYQDFLNAYNRSRLSQIKHAKLDRNQVMPSGVHDRKGERVNVLRQILDSKKSIQAELNEHVKQSLIDSTTAPSTPTKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3KN70</accession>
    <name>E3KN70_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="6 7">40S ribosomal protein S13</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_11035</name>
    </gene>
    <organism evidence="6 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <reference evidence="6" key="3">
        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EFP85706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EHS63777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330125.1">
        <property type="nucleotide sequence ID" value="XM_003330077.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889480.1">
        <property type="nucleotide sequence ID" value="XM_003889431.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KN70"/>
    <dbReference type="SMR" id="E3KN70"/>
    <dbReference type="FunCoup" id="E3KN70">
        <property type="interactions" value="451"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KN70"/>
    <dbReference type="GeneID" id="10544272"/>
    <dbReference type="KEGG" id="pgr:PGTG_11035"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11035"/>
    <dbReference type="eggNOG" id="KOG0400">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090139_1_0_1"/>
    <dbReference type="InParanoid" id="E3KN70"/>
    <dbReference type="OMA" id="MHTRRKG"/>
    <dbReference type="OrthoDB" id="623277at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.10:FF:000003">
        <property type="entry name" value="40S ribosomal protein S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.130:FF:000001">
        <property type="entry name" value="40S ribosomal protein S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01343_A">
        <property type="entry name" value="Ribosomal_uS15_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_uS15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023029">
        <property type="entry name" value="Ribosomal_uS15_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012606">
        <property type="entry name" value="Ribosomal_uS15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="uS15_NS1_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006331">
        <property type="entry name" value="PRK08561.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885">
        <property type="entry name" value="RIBOSOMAL PROTEIN S15P/S13E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885:SF6">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08069">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01386">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="S15/NS1 RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00362">
        <property type="entry name" value="RIBOSOMAL_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS15 N-terminal" evidence="5">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP85706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS63777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="151" mass="17072" checksum="2694AB6DDBDA6FF1" modified="2011-01-11" version="1">MGRMHNPHKGIAGSALPYKRTPARWLKVTAQEVSEQIFKLARKGMTPSQIGVVLRDSHGIAQVKSVTGAKILRILKGNGLAPEIPEDLYHLIKKAVSVRKHLERNRKDKDSKFRLILIESRIHRLTRYYKTKSQLSPSFKYESATASTLVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNV6</accession>
    <name>E3KNV6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glutaredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_11737</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178297">
        <property type="protein sequence ID" value="EFP85981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330400.1">
        <property type="nucleotide sequence ID" value="XM_003330352.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNV6"/>
    <dbReference type="STRING" id="418459.E3KNV6"/>
    <dbReference type="GeneID" id="10539980"/>
    <dbReference type="KEGG" id="pgr:PGTG_11737"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11737"/>
    <dbReference type="eggNOG" id="KOG1752">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1540820_0_0_1"/>
    <dbReference type="InParanoid" id="E3KNV6"/>
    <dbReference type="OMA" id="YDINPAP"/>
    <dbReference type="OrthoDB" id="418495at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015038">
        <property type="term" value="F:glutathione disulfide oxidoreductase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03419">
        <property type="entry name" value="GRX_GRXh_1_2_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002109">
        <property type="entry name" value="Glutaredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011899">
        <property type="entry name" value="Glutaredoxin_euk/vir"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014025">
        <property type="entry name" value="Glutaredoxin_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02180">
        <property type="entry name" value="GRX_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694">
        <property type="entry name" value="GLUTAREDOXIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45694:SF26">
        <property type="entry name" value="GRX1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00462">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00160">
        <property type="entry name" value="GLUTAREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Glutaredoxin" evidence="2">
        <location>
            <begin position="26"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP85981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="175" mass="19869" checksum="F4E1C5701DB8D9B5" modified="2011-01-11" version="1">MEVKEEVDEVDETRKRKQTAIKESAILVYSKSYCPHSRRAKTILARVPDKPSEARVIELDELGERGVQMQSYLAELTHQRTVPNIFIHQKHIGGADDLSHLDQAGVLRSLIVDHDSSRDTKSSSRIFKRQPDLKTLHEGVSYPLLLLLLLVILAGIGYRIKSRAWIAPVPQKQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>E3KP69</accession>
    <name>E3KP69_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein LST8 homolog</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Target of rapamycin complex subunit LST8</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_12050</name>
        <name evidence="11" type="ORF">PGTG_17550</name>
    </gene>
    <organism evidence="10 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <reference evidence="10" key="3">
        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat LST8 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178298">
        <property type="protein sequence ID" value="EFP86094.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="DS178350">
        <property type="protein sequence ID" value="EFP91694.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330513.2">
        <property type="nucleotide sequence ID" value="XM_003330465.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336113.2">
        <property type="nucleotide sequence ID" value="XM_003336065.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KP69"/>
    <dbReference type="FunCoup" id="E3KP69">
        <property type="interactions" value="298"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KP69"/>
    <dbReference type="GeneID" id="10531633"/>
    <dbReference type="GeneID" id="10540100"/>
    <dbReference type="KEGG" id="pgr:PGTG_12050"/>
    <dbReference type="KEGG" id="pgr:PGTG_17550"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12050"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17550"/>
    <dbReference type="eggNOG" id="KOG0315">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_5_1"/>
    <dbReference type="InParanoid" id="E3KP69"/>
    <dbReference type="OrthoDB" id="400at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031932">
        <property type="term" value="C:TORC2 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051897">
        <property type="term" value="P:positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032956">
        <property type="term" value="P:regulation of actin cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032535">
        <property type="term" value="P:regulation of cellular component size"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0038203">
        <property type="term" value="P:TORC2 signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000505">
        <property type="entry name" value="Blast:Protein LST8 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037588">
        <property type="entry name" value="MLST8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19842">
        <property type="entry name" value="G BETA-LIKE PROTEIN GBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19842:SF0">
        <property type="entry name" value="TARGET OF RAPAMYCIN COMPLEX SUBUNIT LST8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 8" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="116"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="157"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="250"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="8">
        <location>
            <begin position="295"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="81"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="99"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00074814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP86094.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP91694.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="355" mass="38691" checksum="6EB6372716021ADA" modified="2012-04-18" version="2">MADPNGLVETGQSNPLDVILATGGYDNTIRFWEAWSGICVKSINHPQSQINRLAISPDKRHLAVAGNAHVRLYDVAIATASNHPAPNQGGSHATSTNDQTPQHQQQAHAQTGLLQSFPHNGNVTSVAWPKESKWFVTGCEDGAMRIWDTRTPVAQRTYDHKCAVNDIAIHPNQGEIISCDQSGAIKIWDLAADSCTHELFPEDDVPQRSVSIASDNSCLVAGNNSGNVFLWNISTSGVGGYTDLQAEATFEAHKRYCLKVLLSPDVRLLATCSADTTIKIWSLTPNQPPKLDKTLYDHQRWVWDLAFSADSAYLVSASSDHSARLWELASGQTVRQYSGHSKPCVAVALNDINVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KP93</accession>
    <name>E3KP93_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12074</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178298">
        <property type="protein sequence ID" value="EFP86118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330537.1">
        <property type="nucleotide sequence ID" value="XM_003330489.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KP93"/>
    <dbReference type="GeneID" id="10540126"/>
    <dbReference type="KEGG" id="pgr:PGTG_12074"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12074"/>
    <dbReference type="HOGENOM" id="CLU_2110172_0_0_1"/>
    <dbReference type="InParanoid" id="E3KP93"/>
    <dbReference type="OrthoDB" id="10402913at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="115" mass="12668" checksum="D27CF8AC45C55AD8" modified="2011-01-11" version="1">MTEGIPPAIETVYSLEGQGSLRPQQAHIPMHKADSSQDYDQPGHHDTIQRGDASQLHYGQLAVAAARSVTQTFQVKLKPHATNAMVCHTTQRNVPEWTMWDWFEADGSGGQARAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPF0</accession>
    <name>E3KPF0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12131</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178298">
        <property type="protein sequence ID" value="EFP86175.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330594.2">
        <property type="nucleotide sequence ID" value="XM_003330546.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KPF0"/>
    <dbReference type="GeneID" id="10540184"/>
    <dbReference type="KEGG" id="pgr:PGTG_12131"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12131"/>
    <dbReference type="HOGENOM" id="CLU_1125008_0_0_1"/>
    <dbReference type="InParanoid" id="E3KPF0"/>
    <dbReference type="OrthoDB" id="2503470at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86175.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="247" mass="27993" checksum="5EC1A007A01D7A98" modified="2012-04-18" version="2">MGSNKHVPGASLWASHITKYKQTSHKCEMLQILENAKTLLNTTRTLILRLKYNSLLWAAIWTLTAAMYTYSVWPLIKMFRVCYKRMEEIKATEQPAMNSKKAPGASKRDRPHERVGIALRRSYIGVHSEHVIVVSEWRALGAWLFLVGGAFSATAMLSQTWRSYIKFDFAQEVSEQVTGLENESETKNKHKWNTLASGSSGNPTLSELVMSTGQHKTESTEDVLVISITEVTRDKIKSQCNIETLRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPR9</accession>
    <name>E3KPR9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Diphthamide biosynthesis protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_12260</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2 [3Fe-4S](0)-[protein] + 2 Fe(2+)-[Dph3] + NADH = 2 [4Fe-4S](1+)-[protein] + 2 [Dph3] + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:71239"/>
            <dbReference type="Rhea" id="RHEA-COMP:17997"/>
            <dbReference type="Rhea" id="RHEA-COMP:17998"/>
            <dbReference type="Rhea" id="RHEA-COMP:18001"/>
            <dbReference type="Rhea" id="RHEA-COMP:18002"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:33723"/>
            <dbReference type="ChEBI" id="CHEBI:47402"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:83228"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[3Fe-4S](1+)-[protein] + Fe(2+)-[Dph3] = [3Fe-4S](0)-[protein] + Fe(3+)-[Dph3]</text>
            <dbReference type="Rhea" id="RHEA:71235"/>
            <dbReference type="Rhea" id="RHEA-COMP:17996"/>
            <dbReference type="Rhea" id="RHEA-COMP:17997"/>
            <dbReference type="Rhea" id="RHEA-COMP:18002"/>
            <dbReference type="Rhea" id="RHEA-COMP:18003"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:33751"/>
            <dbReference type="ChEBI" id="CHEBI:47402"/>
            <dbReference type="ChEBI" id="CHEBI:83228"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Component of the 2-(3-amino-3-carboxypropyl)histidine synthase complex composed of DPH1, DPH2, DPH3 and a NADH-dependent reductase, predominantly CBR1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DPH3 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178299">
        <property type="protein sequence ID" value="EFP86304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330723.1">
        <property type="nucleotide sequence ID" value="XM_003330675.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KPR9"/>
    <dbReference type="FunCoup" id="E3KPR9">
        <property type="interactions" value="152"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KPR9"/>
    <dbReference type="GeneID" id="10540317"/>
    <dbReference type="KEGG" id="pgr:PGTG_12260"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12260"/>
    <dbReference type="eggNOG" id="KOG2923">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_155991_1_0_1"/>
    <dbReference type="InParanoid" id="E3KPR9"/>
    <dbReference type="OMA" id="IADEQPR"/>
    <dbReference type="OrthoDB" id="66964at2759"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.660.10:FF:000001">
        <property type="entry name" value="Diphthamide biosynthesis 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.660.10">
        <property type="entry name" value="DPH Zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044248">
        <property type="entry name" value="DPH3/4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007872">
        <property type="entry name" value="DPH_MB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036671">
        <property type="entry name" value="DPH_MB_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21454:SF31">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21454">
        <property type="entry name" value="DPH3 HOMOLOG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05207">
        <property type="entry name" value="Zn_ribbon_CSL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144217">
        <property type="entry name" value="CSL zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51074">
        <property type="entry name" value="DPH_MB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DPH-type MB" evidence="10">
        <location>
            <begin position="4"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="66"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="66"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00024032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00036267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00041070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP86304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="98" mass="11231" checksum="5AB02B0781B11CCD" modified="2011-01-11" version="1">MVSYYDEIEIEDFTWDEKAKVYHTPCPCGDRFEISKSQLAKGVEIATCPSCSLIVRVVYDMLDFEDYESDEEEEENSDSSSIDEKSIPIEDSLSQLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQ38</accession>
    <name>E3KQ38_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Eukaryotic elongation factor-2 kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12369</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178300">
        <property type="protein sequence ID" value="EFP86413.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330832.2">
        <property type="nucleotide sequence ID" value="XM_003330784.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQ38"/>
    <dbReference type="GeneID" id="10540428"/>
    <dbReference type="KEGG" id="pgr:PGTG_12369"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12369"/>
    <dbReference type="HOGENOM" id="CLU_1152254_0_0_1"/>
    <dbReference type="InParanoid" id="E3KQ38"/>
    <dbReference type="OrthoDB" id="10270247at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="62"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86413.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="241" mass="26601" checksum="87C7BC6DD4C387C0" modified="2012-04-18" version="2">MPGNVAPQLPSQPPNHYNIIPSYPPAAAPTSSIFQALPANPTPPAANLAQCAGSNTHIRNISKSKRTKNPAPTPYSRNPKPPVSATLSESPIRMFKCGISFFQDKNQKKTGIMSHMQQIDTSLLKLYDTLQHQLWQLFSSKLLRKEIVEGLPKIPIGRTSLSHNKSVLDPDTLLLLVGQLTLKKPVQIDLIYEDNGEDTNTILATKKLAATERSLRSQQSAAPSESHHHLQVRLFPVRLLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQJ8</accession>
    <name>E3KQJ8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12955</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178301">
        <property type="protein sequence ID" value="EFP86573.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330992.2">
        <property type="nucleotide sequence ID" value="XM_003330944.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQJ8"/>
    <dbReference type="STRING" id="418459.E3KQJ8"/>
    <dbReference type="GeneID" id="10540673"/>
    <dbReference type="KEGG" id="pgr:PGTG_12955"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12955"/>
    <dbReference type="HOGENOM" id="CLU_1315959_0_0_1"/>
    <dbReference type="InParanoid" id="E3KQJ8"/>
    <dbReference type="OrthoDB" id="10386356at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039977">
        <property type="entry name" value="Suv4-20/Set9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12977:SF4">
        <property type="entry name" value="HISTONE-LYSINE N-METHYLTRANSFERASE KMT5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12977">
        <property type="entry name" value="SUPPRESSOR OF VARIEGATION 4-20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="104"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="174"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86573.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="209" mass="22911" checksum="D7D28428C661CAF7" modified="2012-04-18" version="2">MSFPPPNPGGYPGIPWDTRDQWPAGRVSFQPAILFALQVQTGELRNLTKATTLIRQLPAVIHYLNNSEPPRLGKHIGLYLQLFLPKCGIRFCKTDRYLVGQVASTTGSPSTCTSSISPKLHSRPRHAVVKERQPKPASRPRRKPRSFPAALDTPSEHSGSLSPVPTPRLIPSHRSNDNIPRDIQPKDDPASSAKSPSTNGHRLLVSSIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>E3KRG6</accession>
    <name>E3KRG6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_12632</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DEAD box helicase family. DEAH subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="DS178303">
        <property type="protein sequence ID" value="EFP86891.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331310.2">
        <property type="nucleotide sequence ID" value="XM_003331262.2"/>
    </dbReference>
    <dbReference type="FunCoup" id="E3KRG6">
        <property type="interactions" value="796"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KRG6"/>
    <dbReference type="GeneID" id="10541643"/>
    <dbReference type="KEGG" id="pgr:PGTG_12632"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12632"/>
    <dbReference type="eggNOG" id="KOG0926">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001832_0_2_1"/>
    <dbReference type="InParanoid" id="E3KRG6"/>
    <dbReference type="OrthoDB" id="10253254at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17982">
        <property type="entry name" value="DEXHc_DHX37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18791">
        <property type="entry name" value="SF2_C_RHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002693">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001555">
        <property type="entry name" value="Putative ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002147">
        <property type="entry name" value="Putative ATP-dependent RNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1080:FF:000045">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1080">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011709">
        <property type="entry name" value="DEAD-box_helicase_OB_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048333">
        <property type="entry name" value="HA2_WH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007502">
        <property type="entry name" value="Helicase-assoc_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18934:SF99">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DHX37-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21010">
        <property type="entry name" value="HA2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07717">
        <property type="entry name" value="OB_NTP_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04408">
        <property type="entry name" value="WHD_HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00847">
        <property type="entry name" value="HA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="434"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="779"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="133"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="217"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="342"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="712"/>
            <end position="749"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="18"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="44"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="227"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="245"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="258"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="345"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="370"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="713"/>
            <end position="727"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="729"/>
            <end position="739"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP86891.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1328" mass="147372" checksum="95DB021336DC12FA" modified="2012-04-18" version="2">MGFSRPRYNQKARSHRSAPQDEQKQKTELDYSRVEAVVDHEELGSSSKPKTMSAVNQETEDRGSMSKKKRKRFDAYVQKKLKKERRNELIQELEKTQSSVKDPSAFISTAHLCSSSVARTQRELKATIAEVESRRRERARGLISSKQKLRSGYHSQSDLEDESDPTPATNPFSGAVEICNASSESNITAKKTPRHPPKPTVTSQPLTVAAHKVAFGSALASGAITTHVKRPVKKKRKSHKPPTVLDDDDDSFDDSESSTHSSSTASDDQDQAQSSSSGSSSSSWPEEEEWTGCDDSLQVEEVLVDTDSPQNQPQPDLPSEPTPRVKGSIRDWANKQLQLAEENKLNSTVSHDLEVTASSSPPETRPVSDVPQSQETKSTKTPQTRCGPLGAPLDLPETPLFLHQKTSHVPLERDEEVIASRSLLPVFAEEHTVMDAIRRHPVVVICGETGSGKTTQVPQFLYEAGWGKSDGDNPGLIGVTQPRRVAAVSMARRVEKEMGLSGQGIVSHQIRYDTTTSSTTKIKFMTDGVILRELAADFLLSKYSVVIVDEAHERSINTDVLIGVLSRIVRLRLKTWMENFSNKNSGQNSAGKTSDTEMKTGICCRPLRLIIMSATLRVSDFTLNSSLFSQPPPVIEIAARQFPVAVHFMRRTPMDYVNEAFTKAAKIHSRLPPGGILIFLTGQLEITKLCRKLEQKFGKKIVEERKARKAALQSKSSTLPCAQSTPSIGERDTNDHGDTPQEPVTQMDGFSSGEAEMINLGKDKHEFAADVDEGQEWEEDPEALDTDEEDDLIVEGVELVEDSDVPMYILPLYSLLPTDQQMKVFEDPPPGTRLVVVATNVAETSLTIPNIRYVIDSGRAKERHYDLSTGIQSFEIDWISKASASQRSGRAGRTGPGHCYRLYSSAVFENYFPQFAKPEIQRMPIDGIVLQMKSMNIDTVTNFPFPTPPDRFALRKSEISLAHLGALSFPHGQSDWFMKGDPGGEGTITELGRAMAKYPLSPRFARMLVSGFQYECLAYVIAMVSILSVGDPFIHESAIDEDNPTDCLDDQAINETELLKNADCKDREIRRQKRRQFFKVQQQFASLGNGVSDLFKVLAVVGAYEFGQGNNPSKFCHENFVRPKAMEEIHKLRAQITRIVQAATIATGLKVEFSPKLMPPSETQLKVLRQLIASAFIDQVAIKASKVKSCQSSSGKSGANNPNRNLVAYRAIGIEEDVFIHPSSVLFDSNVHPEPEFIVFQELHRTEKRIWIKGITLINPSWLPVLGKSLCTFSKPIDNSTNNKIPSRATDTERKCFVIPKFGGPGLDVELGPVQMTQRKVGNLWVWV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KRL7</accession>
    <name>E3KRL7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_12683</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178303">
        <property type="protein sequence ID" value="EFP86942.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331361.2">
        <property type="nucleotide sequence ID" value="XM_003331313.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KRL7"/>
    <dbReference type="GeneID" id="10541855"/>
    <dbReference type="KEGG" id="pgr:PGTG_12683"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12683"/>
    <dbReference type="HOGENOM" id="CLU_054993_0_0_1"/>
    <dbReference type="InParanoid" id="E3KRL7"/>
    <dbReference type="OrthoDB" id="2510939at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP86942.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="310" mass="34750" checksum="0B5B9EF80A918886" modified="2012-04-18" version="2">MRIHGKVWTYHNENDFMNPAGSSEVDPNKSSLQYRTSSNSGNFLLSLDHSHTQKSSTTILGLENSNNEVNHNLLKKILILDFDVDSPADQQAAMVYSAVSIHGKASQTQASLSDVHPIGVTKVQKIRLGDLIKKFIADLSAHDKKLLLPTGFIDRNQAVLQAVREQIQETIKSKKGTLQGCCFEFRSGDVPTISKLYNHIQQHLPYGGEFFAPNKIPLIAQVRFTYMRLEMIIYALGTSSARAAQWGPINKQLQYLKTKGADYFIVWSNLIIQNDKYFFGSPKQFAQLRLDVSVLPTKDDIQQAMATSCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KS86</accession>
    <name>E3KS86_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13380</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EFP87161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331580.1">
        <property type="nucleotide sequence ID" value="XM_003331532.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KS86"/>
    <dbReference type="GeneID" id="10537391"/>
    <dbReference type="KEGG" id="pgr:PGTG_13380"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13380"/>
    <dbReference type="HOGENOM" id="CLU_2039212_0_0_1"/>
    <dbReference type="InParanoid" id="E3KS86"/>
    <dbReference type="OrthoDB" id="2510172at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172805" description="Secreted protein" evidence="1">
        <location>
            <begin position="23"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="121" mass="13385" checksum="02620B5038DFA8F4" modified="2011-01-11" version="1">MHNKHLGYVALLCAPLANVVFSVGLLCNYCNLRNLHLTPTITEAPCDQTEQHVILPQYYKPLNCDQCGTGGSLHWTGVTASYGCGTFRHQSACNNPNYCSANVMVRQYKCEAIQITGFPFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KS88</accession>
    <name>E3KS88_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13382</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EFP87163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331582.1">
        <property type="nucleotide sequence ID" value="XM_003331534.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KS88"/>
    <dbReference type="GeneID" id="10537393"/>
    <dbReference type="KEGG" id="pgr:PGTG_13382"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13382"/>
    <dbReference type="HOGENOM" id="CLU_842340_0_0_1"/>
    <dbReference type="InParanoid" id="E3KS88"/>
    <dbReference type="OrthoDB" id="10595419at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="330" mass="38526" checksum="90D8A12620F8D61D" modified="2011-01-11" version="1">MFNSQVANNSQDRSNPRKRRGTDLALEEYEIKPFSSKTAKLLAQRQDKSAYVFKLGKKEEPFSKIKQESNTFEQKVQKVEDYHPDDFFDVYNWDYVREFPEKETTCAEGDKPKYTLQKLVDDLNKCKPLSQRDHFFWIPRKDATRILRKYNKTGLCLPNRFSKSSKRNSIAKIVLRLSDKRLAIEEYPTLSEGILKPLELSVKAKMDKLQHEKGSYYSEKSASQKIQSIIEYVKKVTKVVPFLIITYLSLFKEHNERIIDPNLVGNLLNFLKEIWKSILESDPTLLGKHSWARINSDLFQLRDLSDQNNTQDNILCKLCNYTSKSGAFVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>E3KTX9</accession>
    <name>E3KTX9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Pyruvate carboxylase</fullName>
            <ecNumber evidence="4 13">6.4.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="23" type="ORF">PGTG_13460</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="24" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="24">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.</text>
    </comment>
    <comment type="function">
        <text evidence="2">Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>hydrogencarbonate + pyruvate + ATP = oxaloacetate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20844"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>biotin</name>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <dbReference type="EC" id="6.4.1.1" evidence="4 13"/>
    <dbReference type="EMBL" id="DS178308">
        <property type="protein sequence ID" value="EFP87674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332093.1">
        <property type="nucleotide sequence ID" value="XM_003332045.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KTX9"/>
    <dbReference type="FunCoup" id="E3KTX9">
        <property type="interactions" value="365"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KTX9"/>
    <dbReference type="GeneID" id="10538906"/>
    <dbReference type="KEGG" id="pgr:PGTG_13460"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13460"/>
    <dbReference type="eggNOG" id="KOG0369">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000395_1_0_1"/>
    <dbReference type="InParanoid" id="E3KTX9"/>
    <dbReference type="OMA" id="AEACICY"/>
    <dbReference type="OrthoDB" id="196847at2759"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004736">
        <property type="term" value="F:pyruvate carboxylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06850">
        <property type="entry name" value="biotinyl_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07937">
        <property type="entry name" value="DRE_TIM_PC_TC_5S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000003">
        <property type="entry name" value="Acetyl-CoA carboxylase biotin carboxyl carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000018">
        <property type="entry name" value="Biotin carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000010">
        <property type="entry name" value="Propionyl-CoA carboxylase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000033">
        <property type="entry name" value="Pyruvate carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000012">
        <property type="entry name" value="Pyruvate carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.600.10">
        <property type="entry name" value="pyruvate carboxylase f1077a mutant domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001882">
        <property type="entry name" value="Biotin_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000089">
        <property type="entry name" value="Biotin_lipoyl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003379">
        <property type="entry name" value="Carboxylase_cons_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055268">
        <property type="entry name" value="PCB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005930">
        <property type="entry name" value="Pyruv_COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011053">
        <property type="entry name" value="Single_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006761">
        <property type="entry name" value="PRK09282.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009554">
        <property type="entry name" value="PRK12999.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01235">
        <property type="entry name" value="pyruv_carbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43778">
        <property type="entry name" value="PYRUVATE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43778:SF2">
        <property type="entry name" value="PYRUVATE CARBOXYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00364">
        <property type="entry name" value="Biotin_lipoyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02436">
        <property type="entry name" value="PYC_OADA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001594">
        <property type="entry name" value="Pyruv_carbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89000">
        <property type="entry name" value="post-HMGL domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51230">
        <property type="entry name" value="Single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00188">
        <property type="entry name" value="BIOTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50968">
        <property type="entry name" value="BIOTINYL_LIPOYL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9 13 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 13" id="KW-0092">Biotin</keyword>
    <keyword evidence="5" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="6 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="8 13 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="21">
        <location>
            <begin position="54"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="20">
        <location>
            <begin position="174"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="22">
        <location>
            <begin position="592"/>
            <end position="861"/>
        </location>
    </feature>
    <feature type="domain" description="Lipoyl-binding" evidence="19">
        <location>
            <begin position="1127"/>
            <end position="1205"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="18">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="18">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="346"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="601"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="673"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="16">
        <location>
            <position position="770"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="800"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="802"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="935"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="17">
        <location>
            <position position="770"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-biotinyllysine" evidence="17">
        <location>
            <position position="1171"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRNR" id="PIRNR001594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001594-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001594-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR001594-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR001594-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="EFP87674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1207" mass="131856" checksum="C70ADF6E181A405F" modified="2011-01-11" version="1">MATHLTADDPNMKKDAFSLHTPASSLPGTPGGTMGHHTPNVQSIRRQTAGHRGPLQKILVANRGEIAIRVFRTAHELAMHTVAIYSHEDRMSAHRHKADEAYMVGKGCTPVGAYLAQDDIIRLALEHGVDMIHPGYGFLAENAVFAKKVEDAGLAFIGPQPEVIDGLGDKVKARTLAIQCGVPVVPGTEGAIASYDLADAFIKEHGFPVIIKAAMGGGGRGMRVVRAAEDFKESFERAVSEAKSAFGDGTVFIERFLDKPRHIEVQLLGDNLGNVIHLFERDCSVQRRHQKVVELAPASNLSDEVRTRILEDAKKLAQAVKYRNAGTAEFLVDQQGRHHFIEINPRIQVEHTITEEITGIDIVAAQIQIAAGATLAELGLTQEAVSKRGHAIQCRVTTEDPAMGFQPDTGKIEVYRSAGGNGVRLDASSGFAGAQITPHYDSLLTKVTVRGATFEIARRKMLRALVEFRIRGVKTNIPFLFRVLSHETFVASQTWTTFIDDTPALFHLISSQNRAQKLLGYLGDLVVNGSSIKGQQGEPGLKDEIVIPTLAQPTDPSQTLDVSQPCQQGWRKILKSEGPAAFAKAVRDYKGCLIMDTTWRDAHQSLLATRLRTIDILNIARETSHALANAFSLECWGGATFDVAMRFLYEDPWERLRKIRKLVPNIPLQALIRGANAVGYTSYPDNAIYEFSKKAVENGLDIFRVFDSLNYLENMKIGIDAAKKAGGVVEGTICYTGDVANPAKSKYTLEYYLKFASELVAEGIHVLGIKDMAGLLTPRAAKILISALREKYPDLPIHVHSHDTAGISLATMLQCAESGADVVDCAIDSMSGMTSQCAMGALCAALEQNGLGTGIRFEDIQALNLYWSQCRLLYSCFDANVKSSDSGVYEHEMPGGQYTNLMFQSQQLGLGTQWAAVKTAYIEANQLCGDIVKVTPSSKVVGDFAQFMVTNKLTRADVEERASKLDFPNSVVEFFQGYLGQPVGGFPEPLRTHIIRDKPRIEGRPGMSLPPYNFEATRKELQDKFGKSITSTDVLSHCMYPKVFEDFREFLSKYGDLSILPTRHFLAKPEVGEEMHIAIEQGKTLIVKLLASGPVNPETGVREVFFELNGETRAVQVEDRSAAVETAHREKATSDPGSVGSPMAGVVVEIRVQEGHEVKAGDPICIMSAMKMEQNVTAPVGGKISRVAIQPGDSIGSGDLIVEIKHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>E3KUQ9</accession>
    <name>E3KUQ9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_13813</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178310">
        <property type="protein sequence ID" value="EFP88009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332428.1">
        <property type="nucleotide sequence ID" value="XM_003332380.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KUQ9"/>
    <dbReference type="GeneID" id="10535068"/>
    <dbReference type="KEGG" id="pgr:PGTG_13813"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13813"/>
    <dbReference type="HOGENOM" id="CLU_2134796_0_0_1"/>
    <dbReference type="InParanoid" id="E3KUQ9"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP88009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="113" mass="12810" checksum="6F081157A0B27880" modified="2011-01-11" version="1">MKKKKTASYDIFLPPNNNNNRQTKKYTFNIFSFCFSRGWLAGRRLARFQVDTLPIHSCSCELGLALLRPPEPRPIAARLITTPIQDARRDPSLASLKPTLLPACQVVRKSDPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3KV76</accession>
    <name>E3KV76_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_14255</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178312">
        <property type="protein sequence ID" value="EFP88171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332590.1">
        <property type="nucleotide sequence ID" value="XM_003332542.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KV76"/>
    <dbReference type="GeneID" id="10535397"/>
    <dbReference type="KEGG" id="pgr:PGTG_14255"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14255"/>
    <dbReference type="HOGENOM" id="CLU_2237933_0_0_1"/>
    <dbReference type="InParanoid" id="E3KV76"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP88171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="105" mass="11812" checksum="6F037E411BCCBD32" modified="2011-01-11" version="1">MTFYSKLTLAINLAFLPETSDVAVKAVDYLKDEGTDLVQERLKVEKNEIIAFYFFGNHNGGNFIFPSLGVALTGLHGYSVHGPFRILYHGVAQYHFKQDIPDAPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVM1</accession>
    <name>E3KVM1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14445</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178313">
        <property type="protein sequence ID" value="EFP88361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332780.1">
        <property type="nucleotide sequence ID" value="XM_003332732.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KVM1"/>
    <dbReference type="GeneID" id="10535834"/>
    <dbReference type="KEGG" id="pgr:PGTG_14445"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14445"/>
    <dbReference type="HOGENOM" id="CLU_066699_0_0_1"/>
    <dbReference type="InParanoid" id="E3KVM1"/>
    <dbReference type="OrthoDB" id="10422926at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="121"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="127"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="289" mass="31981" checksum="3F66B48128D1FBF5" modified="2011-01-11" version="1">MHHLMILRSNVNQSPLLRFPGSSESVIVLDSDPRPPLRHTIQVDTADPTKSLEDGRPTKRQRLHEAPSDAPEATASTSTNVNPPTPGGPPKDIVDESIAALLNAPVNPDEFWSIFSDIPEASTSVPDHSRVKEPRPPENSKNGDATRPIANAPKPDIDLSDQHGARKKNPIEVIAPTRPPQLAVSEGAPYPQSTKGIGPIPSYFYADVIPFYKSSQTKISKIYNGIVSHVPKTRWNDFEFCEGTKLRDKWFEYVPADMIFSTSPTSNTKLERMVWILDLSGTHDLLVWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVW5</accession>
    <name>E3KVW5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14018</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178314">
        <property type="protein sequence ID" value="EFP88440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332859.1">
        <property type="nucleotide sequence ID" value="XM_003332811.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KVW5"/>
    <dbReference type="GeneID" id="10540742"/>
    <dbReference type="KEGG" id="pgr:PGTG_14018"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14018"/>
    <dbReference type="HOGENOM" id="CLU_1455096_0_0_1"/>
    <dbReference type="InParanoid" id="E3KVW5"/>
    <dbReference type="OrthoDB" id="2500537at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041320">
        <property type="entry name" value="CxC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18802">
        <property type="entry name" value="CxC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxC1-like cysteine cluster associated with KDZ transposases" evidence="1">
        <location>
            <begin position="73"/>
            <end position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="186" mass="20889" checksum="5ABDEE620426BF6B" modified="2011-01-11" version="1">MSSKSNNGKTYMDQCSPCFTIVQQTRPTGVMSKYGTVIGNQHVAVLRPVKDLQYSWISSAVPSKAHPDGVHWCNPQSPKTAFSIQLLQLHHILWKHCTIAMLPSSKAIDEFPDINNPLILVPHGEDDLEESRKQLGQAVDAFQEMLKRENKLLAQVMRLEPLGELADICPKWYGQQVDGKREGQPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>E3KWT2</accession>
    <name>E3KWT2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_14715</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178316">
        <property type="protein sequence ID" value="EFP88749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333168.1">
        <property type="nucleotide sequence ID" value="XM_003333120.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KWT2"/>
    <dbReference type="STRING" id="418459.E3KWT2"/>
    <dbReference type="GeneID" id="10541700"/>
    <dbReference type="KEGG" id="pgr:PGTG_14715"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14715"/>
    <dbReference type="eggNOG" id="ENOG502SUPF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_055324_1_1_1"/>
    <dbReference type="InParanoid" id="E3KWT2"/>
    <dbReference type="OMA" id="YTETANF"/>
    <dbReference type="OrthoDB" id="2506362at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023:SF4">
        <property type="entry name" value="GLYCINE-RICH PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="2">
        <location>
            <begin position="109"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="144"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP88749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="344" mass="39063" checksum="AFFD3B6BBE2CBA74" modified="2011-01-11" version="1">MPSKRGPNWLPPDDEQLAKSWLKISEDVIRSTGQKKEEFWKRVADDFNKFSTSVAREGTHCMHHWGHVQKWTLKFSGIYRKLEKTRPSGSVMSDLLPDARKAFYEQEGKQFAFERAWMVLKDSPKWNDASESRGSGGAQPSELPVSTPTPSTPTNQANPATIPSQSSALPQMGSDGSWKRPPGVHSTKRAMKEDHYNAKKIKILSNRSTDYRDRTLAMKKTNDIRDRAAEAELRSSNMEIMSKRIEDLPDDISRKYLELQKEIIMHDIRDQVEERRKLAAEKSKGINQSASSSSHQEPQINQSASSSSHPESRDSENESSETEENEEVAEVYEDEIDPTLDDLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KX93</accession>
    <name>E3KX93_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_15169</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178318">
        <property type="protein sequence ID" value="EFP88966.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333385.2">
        <property type="nucleotide sequence ID" value="XM_003333337.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KX93"/>
    <dbReference type="GeneID" id="10542407"/>
    <dbReference type="KEGG" id="pgr:PGTG_15169"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15169"/>
    <dbReference type="HOGENOM" id="CLU_2027834_0_0_1"/>
    <dbReference type="InParanoid" id="E3KX93"/>
    <dbReference type="OrthoDB" id="2507902at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174209" description="Secreted protein" evidence="2">
        <location>
            <begin position="20"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP88966.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="13087" checksum="CE3012D71512C1DD" modified="2012-04-18" version="2">MNTLSTCLIILSVFGFSHAADIQNPKGYTIRNGQFSADTQEGTTVQCAACRLFNASGCHPLNNVQAPRTSQLCTKSYIFTGPSSGRCYTDQDSYLCDKGHTEKRSPGQMPGCQGCGAEDPPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>E3KXH6</accession>
    <name>E3KXH6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">SRP54-type proteins GTP-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_15137</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GTP-binding SRP family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178318">
        <property type="protein sequence ID" value="EFP88934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333353.1">
        <property type="nucleotide sequence ID" value="XM_003333305.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KXH6"/>
    <dbReference type="FunCoup" id="E3KXH6">
        <property type="interactions" value="333"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KXH6"/>
    <dbReference type="GeneID" id="10542214"/>
    <dbReference type="KEGG" id="pgr:PGTG_15137"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15137"/>
    <dbReference type="eggNOG" id="KOG0781">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009301_8_1_1"/>
    <dbReference type="InParanoid" id="E3KXH6"/>
    <dbReference type="OMA" id="HLGWIDK"/>
    <dbReference type="OrthoDB" id="1727884at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005785">
        <property type="term" value="C:signal recognition particle receptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005047">
        <property type="term" value="F:signal recognition particle binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14826">
        <property type="entry name" value="SR_alpha_SRX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17876">
        <property type="entry name" value="SRalpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000188">
        <property type="entry name" value="signal recognition particle receptor subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.140:FF:000026">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007222">
        <property type="entry name" value="Sig_recog_particle_rcpt_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134:SF1">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04086">
        <property type="entry name" value="SRP-alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="Domain of the SRP/SRP receptor G-proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0675">Receptor</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="9">
        <location>
            <begin position="664"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="183"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="190"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="239"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="322"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP88934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="691" mass="76649" checksum="472E37EF8C6BC559" modified="2011-01-11" version="1">MGRDIPGACVGPCGTPGEDQPTHSCSRNTLKYLGELMLDHVHIFHRGGLVLWSKSFVPTPSPIRTLIGDGLIGEQSSGLNDLMVMQIGSYSLQWNFSNEFGLIFVVAYQRILQLTYITDLLESMKSLFTSLYGSILDTTFNSWIVPETGLTEGFKDLFKGWDQSFHKLLKDIERMSDRRRGGIDFQKTSGLDKRAAQRQAEEQQLVDDQKPTALTTATDAETIARNIAALKARKKPNKSSTSRATSRSGTDTDTAPAGKPQTTNKKTARKWDNGRITANDIAEYDFSEKDAHLDDSKTLSSNFIDKMSMGTRNQKGLYEVADFDRSRPDQKSEEDKSQPTLFSKIISSIPIFGGQKSGPITLREEDVKPMIEQIQLQLMKKNVAREIAVRICERIQVELIGQKLSDRSTTALKKLINHSLEISLTKILTPKTSVDILADIHQKQDRQKQMNLSQLSGGTSTAPYVMTFVGVNGVGKSTNLSKVAFWLLQNRLRVLIAACDTFRSGAVEQLRTHVRNLGKLDGAIEHQSPNSNHEERKMIELFEKGYGKDAAGIAKEAIQYAKNDGFDVVLIDTAGRMQDNEPLMRALGKLVVVNQPDKILFVGEALVGNEAVDQLGKFDKSLKTFSGLDSHLPRGIDGIILTKFDTIDDKVGAALSMTYTINQPILFVGTGQTYTDLKNLKVNHVVNALMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3KXS0</accession>
    <name>E3KXS0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_14973</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Protein modification; protein ubiquitination.</text>
    </comment>
    <dbReference type="EMBL" id="DS178319">
        <property type="protein sequence ID" value="EFP89132.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333551.2">
        <property type="nucleotide sequence ID" value="XM_003333503.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KXS0"/>
    <dbReference type="GeneID" id="10546684"/>
    <dbReference type="KEGG" id="pgr:PGTG_14973"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14973"/>
    <dbReference type="eggNOG" id="KOG0800">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2085976_0_0_1"/>
    <dbReference type="InParanoid" id="E3KXS0"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024766">
        <property type="entry name" value="Znf_RING_H2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12678">
        <property type="entry name" value="zf-rbx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0833">Ubl conjugation pathway</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="7">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP89132.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="117" mass="13538" checksum="C4BFD011C6EF4D4E" modified="2012-04-18" version="2">MNWPSCDHLFHQDCVGEWRNRNETCPLCRGKDEKLIEENAAHRAQRNEEESADEARSARIMEHFRRRLLQIGTPSNALEEIEVARLTRLFQARENAYDLTLSDLGTMDQAQMPAPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3KY01</accession>
    <name>E3KY01_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar sorting protein VPS33/slp1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15043</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the STXBP/unc-18/SEC1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178320">
        <property type="protein sequence ID" value="EFP89202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333621.1">
        <property type="nucleotide sequence ID" value="XM_003333573.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KY01"/>
    <dbReference type="FunCoup" id="E3KY01">
        <property type="interactions" value="528"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KY01"/>
    <dbReference type="GeneID" id="10546839"/>
    <dbReference type="KEGG" id="pgr:PGTG_15043"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15043"/>
    <dbReference type="eggNOG" id="KOG1302">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016678_3_1_1"/>
    <dbReference type="InParanoid" id="E3KY01"/>
    <dbReference type="OMA" id="EFHIFFV"/>
    <dbReference type="OrthoDB" id="10262287at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033263">
        <property type="term" value="C:CORVET complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.850">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2060">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.830.10">
        <property type="entry name" value="Syntaxin Binding Protein 1, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043154">
        <property type="entry name" value="Sec-1-like_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043127">
        <property type="entry name" value="Sec-1-like_dom3a"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001619">
        <property type="entry name" value="Sec1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027482">
        <property type="entry name" value="Sec1-like_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036045">
        <property type="entry name" value="Sec1-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043155">
        <property type="entry name" value="VPS33_dom3b"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11679">
        <property type="entry name" value="VESICLE PROTEIN SORTING-ASSOCIATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00995">
        <property type="entry name" value="Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005715">
        <property type="entry name" value="VPS45_Sec1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56815">
        <property type="entry name" value="Sec1/munc18-like (SM) proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="663" mass="72565" checksum="34607C8B059D459C" modified="2011-01-11" version="1">MAMTTGSSTPTQNPSSSSFNRHLTSLDFSIFKTYLVRSLTDQLDNILGPKTIIIDPVLAGPLGLVTQVGNLKEHGVDKLFWLEKGPLGNAGTAQNIVWVCQPMVSAMKLIAQQILSLPARSSNSYTILMTPRATQISKNALSEAGVLGSVEVKEFDLNFVPLEKDVFSLEEEFGGYSQIFADGDLNPIYNMARAIMTIQRAFGLIPRLIGKGTASRKLANLLHRLKSEYVSSPGHGTTMPKLTPGSIDSIIILDRQLDMVTPMCTQLTYEGLLDEIFGIKHAHIEVAPDLLAPSTPVAGNTAHSIPSSSTSQTFPQVAKRKKHVLSSSSDPIFSELRDQNFSIVGNSLNRIAKRINMDYERRNEARTVQQIREFVGRLPELQNEHQALRLHTGLSEQIMTTTTSDEFNQTLEIQQNLVAGLDLSGQEKAISQLIDQEVPLRTILRLLCLYSSVSGGIKPKSFESLKRDLLQTYGYQHVNLLIKLSTLSLLSSTTTGGPSSVPGSIMSGMTLIGGRSGFSGARKPLKLIVDELDGPEPNDISYSYSGYAPLSVRIIESICFNKSLGWKSSVEELLKSFPGEAFDERQIDADSSDGRPSSIDPIPTTIICYLGGITYAEISSLRFTFSNHNQNNSTNKRKLIILTTGIINGNQVLSSLMPVETRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZD2</accession>
    <name>E3KZD2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Vacuolar ATPase assembly protein VMA22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_15620</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178324">
        <property type="protein sequence ID" value="EFP89657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334076.1">
        <property type="nucleotide sequence ID" value="XM_003334028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZD2"/>
    <dbReference type="STRING" id="418459.E3KZD2"/>
    <dbReference type="GeneID" id="10545223"/>
    <dbReference type="KEGG" id="pgr:PGTG_15620"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15620"/>
    <dbReference type="eggNOG" id="ENOG502R7TW">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1147396_0_0_1"/>
    <dbReference type="InParanoid" id="E3KZD2"/>
    <dbReference type="OMA" id="RMEPRVC"/>
    <dbReference type="OrthoDB" id="2507707at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990871">
        <property type="term" value="C:Vma12-Vma22 assembly complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070072">
        <property type="term" value="P:vacuolar proton-transporting V-type ATPase complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040357">
        <property type="entry name" value="Vma22/CCDC115"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31996">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31996:SF2">
        <property type="entry name" value="COILED-COIL DOMAIN-CONTAINING PROTEIN 115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21730">
        <property type="entry name" value="Vma22_CCDC115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="122"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="123"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="167"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00093634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP89657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="249" mass="27777" checksum="A4F15563E78AC83D" modified="2011-01-11" version="1">MARSTEFQLTGSLSKKWAANSANKVEVLDKYLLTYLNLLDDYNACHDGLQSHIQAAFLNLSKAKVVLGPSRIGPNSYDLSSRLPTKTIVIQNQDPIDPDEELDQSKGPFMLTLNRFETCPSVELTNDPSGCSSENGLRKRKNKQEPVSPEPGLPTEEHAAVQANSLPEKEDQDKDRPKELPNPIHQFAALPPPPLRSAQIGFSKTLESIVRLSNLRSVLIEIEKFIELQRQLVENQSNSDPPERENLQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZF6</accession>
    <name>E3KZF6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15644</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178324">
        <property type="protein sequence ID" value="EFP89681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334100.1">
        <property type="nucleotide sequence ID" value="XM_003334052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZF6"/>
    <dbReference type="GeneID" id="10545248"/>
    <dbReference type="KEGG" id="pgr:PGTG_15644"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15644"/>
    <dbReference type="HOGENOM" id="CLU_2293079_0_0_1"/>
    <dbReference type="InParanoid" id="E3KZF6"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="58"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="101" mass="11815" checksum="302595FC60B61657" modified="2011-01-11" version="1">MSLPLYSAPFYQWFTSFATQILRLQQRKPGIHLLYQSSALYDQNLDLITSSKKNPKHAFLATINKALCLVTTLFLAFRVLARLLSWQWLRRLLDRCELNSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZJ6</accession>
    <name>E3KZJ6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_15377</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178325">
        <property type="protein sequence ID" value="EFP89721.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334140.2">
        <property type="nucleotide sequence ID" value="XM_003334092.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZJ6"/>
    <dbReference type="GeneID" id="10545290"/>
    <dbReference type="KEGG" id="pgr:PGTG_15377"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15377"/>
    <dbReference type="HOGENOM" id="CLU_837120_0_0_1"/>
    <dbReference type="InParanoid" id="E3KZJ6"/>
    <dbReference type="OrthoDB" id="10682236at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP89721.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="332" mass="38052" checksum="59271D2AC1D02B79" modified="2012-04-18" version="2">MINFGPSDLLDIRKGKLAAVAAFVSYPASPPRRKRGVLSWVNKRSKIILEQLERLRTSLSAKSPAKQDFYLAQAEKEIPLAGISRFTFDWNVPRDEDSAWNSVVIEVMGKKSVEWLRRTMKISEEEEAQAAAIIKRWLQTKSREIQQYGEMDITEYNKIKSKKSTKALYMRWRKKIKEERCRVADQIFKGIPQLAVVLEDKDCHSDIEDAQEGVNPVRVFPGYRSIHLTNILHNLDRMVQAQTTHHKKIETNKKMYARSASNHAPTVGGAIGVARDWPIDCYDETFWKCSANLSVTLSQKYRQSTFNSLRKPWPKCAVGALAAVRVVNRTKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZN9</accession>
    <name>E3KZN9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">SGS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_15420</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178325">
        <property type="protein sequence ID" value="EFP89764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334183.1">
        <property type="nucleotide sequence ID" value="XM_003334135.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZN9"/>
    <dbReference type="FunCoup" id="E3KZN9">
        <property type="interactions" value="540"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KZN9"/>
    <dbReference type="GeneID" id="10545415"/>
    <dbReference type="KEGG" id="pgr:PGTG_15420"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15420"/>
    <dbReference type="eggNOG" id="KOG1309">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039532_2_0_1"/>
    <dbReference type="InParanoid" id="E3KZN9"/>
    <dbReference type="OMA" id="KVHMTAD"/>
    <dbReference type="OrthoDB" id="1898560at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051087">
        <property type="term" value="F:protein-folding chaperone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06466">
        <property type="entry name" value="p23_CS_SGT1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.790:FF:000012">
        <property type="entry name" value="SGT1 homolog, MIS12 kinetochore complex assembly cochaperone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007052">
        <property type="entry name" value="CS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007699">
        <property type="entry name" value="SGS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044563">
        <property type="entry name" value="Sgt1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45862">
        <property type="entry name" value="PROTEIN SGT1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04969">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05002">
        <property type="entry name" value="SGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51203">
        <property type="entry name" value="CS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51048">
        <property type="entry name" value="SGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CS" evidence="3">
        <location>
            <begin position="6"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="SGS" evidence="2">
        <location>
            <begin position="131"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP89764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="25875" checksum="4628C1DD35CF543A" modified="2011-01-11" version="1">MSNQTQSRIRHEWYQTDTEVVLSVFIKNTRTEDVKCEFGERSVSLNHKKSPDNPSSSSSFSETCFDLDPLSYDIQPTLSSWKSLSSKIDIRLKKKVNGIKWQVLEGDGRDVPAPTVTQLEDPSASVSRQSAYPSSARRKTNWDQLANSVEKEEEEEVKNLKDPNAGGDRAINEVFQKLYADATDEQKKAMMKSYVESNGTALSTDWADVSQKKIDTRPPDSMVAKSWKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZP5</accession>
    <name>E3KZP5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="18">Peptidyl-prolyl cis-trans isomerase cyp8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGTG_15426</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">May catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides thereby assisting the folding of proteins. May also function as a chaperone, playing a role in intracellular transport of proteins. May also have a protein ubiquitin ligase activity acting as an E3 ubiquitin protein ligase or as a ubiquitin-ubiquitin ligase promoting elongation of ubiquitin chains on proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.27"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the cyclophilin-type PPIase family. PPIL2 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178325">
        <property type="protein sequence ID" value="EFP89770.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334189.2">
        <property type="nucleotide sequence ID" value="XM_003334141.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZP5"/>
    <dbReference type="FunCoup" id="E3KZP5">
        <property type="interactions" value="577"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KZP5"/>
    <dbReference type="GeneID" id="10545421"/>
    <dbReference type="KEGG" id="pgr:PGTG_15426"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15426"/>
    <dbReference type="eggNOG" id="KOG0883">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_7_0_1"/>
    <dbReference type="InParanoid" id="E3KZP5"/>
    <dbReference type="OrthoDB" id="407558at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16663">
        <property type="entry name" value="RING-Ubox_PPIL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000079">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000014">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase cyp65"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044666">
        <property type="entry name" value="Cyclophilin_A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026951">
        <property type="entry name" value="PPIL2_U-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003613">
        <property type="entry name" value="Ubox_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45625:SF1">
        <property type="entry name" value="RING-TYPE E3 UBIQUITIN-PROTEIN LIGASE PPIL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00504">
        <property type="entry name" value="Ubox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51698">
        <property type="entry name" value="U_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0697">Rotamase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="U-box" evidence="15">
        <location>
            <begin position="38"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="14">
        <location>
            <begin position="301"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="460"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="224"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00007930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EFP89770.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="18">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="566" mass="63014" checksum="116F71CA3AE51408" modified="2012-04-18" version="2">MGHGSDKLYITHREHAGEFGDHHASSAGAKKKAGSNFQRLPFDCCAISLKPFEMPMCTPDGSTVDLINIIPYIKKYARDPVTGSPLASKDLIKLNFFKNAEDNYYDPVTYKVFNEHTHIVAIKTSGNVFSRESIDRLNIKPGHWHDLVTDEKFSRADIITLQDPHNIEKKDLSRFDHLKKNLKVPNEEIEAMRKDPMRGLNVAAAGSTVSAVLKELAKDKETSKSASTNATPTQQGLVAESSIAPSKGLTPYNASVFSTGKAAASFTSTSLTPATKTEVEIIDEEDFMFSHIKEKGYVKISTNLGDLNVELYCDRAPRTCYNFFMLARKGYYDNTEFHRLIPGFMIQGGDPTGTGKGGESYWGKPFGDEFHLRNAYKHTSRGMLSMANHGKDTNGSQFFITFRETPHLNNKHTVFGRVVGGDSVLSKFESIPIDQVTDKPLKLLKINTILIFDDPFEKYKERLSKRLQKEKSEVEAVEEKLKRKEIREKDRITWFGTTLSDAAKKEQTVVGSSTGGSTVGKYLNQNNSGKLTGTQKRPGDLVAMGEEIGVKQAAKRRKGFGDLSGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3L005</accession>
    <name>E3L005_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15842</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178326">
        <property type="protein sequence ID" value="EFP89886.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334305.2">
        <property type="nucleotide sequence ID" value="XM_003334257.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L005"/>
    <dbReference type="GeneID" id="10545700"/>
    <dbReference type="KEGG" id="pgr:PGTG_15842"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15842"/>
    <dbReference type="HOGENOM" id="CLU_2980163_0_0_1"/>
    <dbReference type="InParanoid" id="E3L005"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89886.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="58" mass="6148" checksum="5C36729E553A0586" modified="2012-04-18" version="2">MAVRRATLHPPSGRKREWTGRDGGAGHGLAQHTLGAGTPPGAKGVWARPNTQERPPRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>E3L2S6</accession>
    <name>E3L2S6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Amidohydrolase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17041</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178338">
        <property type="protein sequence ID" value="EFP90842.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335261.2">
        <property type="nucleotide sequence ID" value="XM_003335213.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L2S6"/>
    <dbReference type="STRING" id="418459.E3L2S6"/>
    <dbReference type="GeneID" id="10530747"/>
    <dbReference type="KEGG" id="pgr:PGTG_17041"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17041"/>
    <dbReference type="eggNOG" id="ENOG502TAAB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_634816_0_0_1"/>
    <dbReference type="InParanoid" id="E3L2S6"/>
    <dbReference type="OrthoDB" id="2135488at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052350">
        <property type="entry name" value="Metallo-dep_Lactonases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43569">
        <property type="entry name" value="AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43569:SF2">
        <property type="entry name" value="AMIDOHYDROLASE-RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="314"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="13"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90842.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="437" mass="49239" checksum="5F24FAFBA43BB138" modified="2012-04-18" version="2">MPKKPPPHLPLQAFSSNPASSEQQANQLLTRRVPLVAIDAHVFLPEDSEAGHSRTSAKLWHDSIQPFYPEQITSAGDHQQAHSHQTTTQFKGFIHIHQQPAWLLEQTPDDPQQAAQLSSKRWETESQNILRIDKELVEIQMAPPLGQVIWAPLHQGPSVLGSYLDQLSNLSIRICGCCHLIERKSTTRNVSLVDPEFIESLKLLGERGLSVELMVDGNHERSARAILDEVLECVSAVRRGQDSKSETKFVLCQMGMPEMISTQSLVPSSASYSSILSRLFSLSLFSDFHIKLSGLPLILEEELNKRACKRFLQSHRPNPRRDLEPSTPVAVHEDEDGGKQETDRDALAQGESAWREVKKRIKFYLEPILEAFGDHRLIYASGFPYYDVPQVSSPADKATGRYEYQFEIFRECLTEFGLENGALDNVFGLNAQRCYSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3L434</accession>
    <name>E3L434_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_17166</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178344">
        <property type="protein sequence ID" value="EFP91309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335728.1">
        <property type="nucleotide sequence ID" value="XM_003335680.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L434"/>
    <dbReference type="GeneID" id="10529139"/>
    <dbReference type="KEGG" id="pgr:PGTG_17166"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17166"/>
    <dbReference type="HOGENOM" id="CLU_109921_0_1_1"/>
    <dbReference type="InParanoid" id="E3L434"/>
    <dbReference type="OMA" id="YRSWAGM"/>
    <dbReference type="OrthoDB" id="2497045at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173094" description="Secreted protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP91309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="223" mass="24512" checksum="3A94DC357D2FBC28" modified="2011-01-11" version="1">MVSLTYLITLLSVLVAVSRAEEVTTVKNENVDAKWRGGYFGFNSMYNMNSWSAYSGLSMQLMGGSWLNNWAGMLPGCSGGLLFPSSWVMNGFFAKAAEEAHSVSRRAITLDAEQLFRRDQVADSATCLNNKGVHEVYSKTDCKNAAKILHEKNVHTASSGNCQLSLYTAKEKVYAKQLPDQMLMKAADRLLSTCGEKASQASSKQSHPEDNQVAMLLSRPSHK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3L4U3</accession>
    <name>E3L4U3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17622</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178348">
        <property type="protein sequence ID" value="EFP91568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335987.1">
        <property type="nucleotide sequence ID" value="XM_003335939.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L4U3"/>
    <dbReference type="GeneID" id="10531505"/>
    <dbReference type="KEGG" id="pgr:PGTG_17622"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17622"/>
    <dbReference type="HOGENOM" id="CLU_047292_0_0_1"/>
    <dbReference type="InParanoid" id="E3L4U3"/>
    <dbReference type="OrthoDB" id="2506484at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="254" mass="27554" checksum="3D9B9CC0BE5BDCDE" modified="2011-01-11" version="1">MKIHLLGYGSAAMALLRDNTYLLSGRLICPNLKTPPLLYYDQDLTFPMGPTESLPISLSNKTAVWGYGIVVSKHERTDSALGQSNFQSLFVVMKHTDYDNQSKTQVSFNVSYKIPGNRNLAKTFGLFQLGREMLLSGTLTGYDKSQKMLQVQALSVSLSTGPDALTTSSVALETSVPQPRKQYQINFDSDDEADTETPIVNSSTSDLIPGPNPTDTPASNADPTTSLPGPKRRYNKKTKTESVTGPTPGSSAIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3L4Z2</accession>
    <name>E3L4Z2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17671</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178349">
        <property type="protein sequence ID" value="EFP91617.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336036.2">
        <property type="nucleotide sequence ID" value="XM_003335988.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L4Z2"/>
    <dbReference type="GeneID" id="10531554"/>
    <dbReference type="KEGG" id="pgr:PGTG_17671"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17671"/>
    <dbReference type="HOGENOM" id="CLU_318096_0_0_1"/>
    <dbReference type="InParanoid" id="E3L4Z2"/>
    <dbReference type="OrthoDB" id="2507823at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="158"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="222"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="298"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="511"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="637"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="691"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="771"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="49"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="537"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="554"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="781"/>
            <end position="801"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="818"/>
            <end position="828"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="854"/>
            <end position="871"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="892"/>
            <end position="911"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91617.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="923" mass="100063" checksum="130582894A00F3BB" modified="2012-04-18" version="2">MNTKNTEKMTQEPSILPDLSNRATQEYSDLFAVSGSSFLTPIDQITINNSNTTIETSSPPLSNRYDTSSLIDLKTPKRTSPTTDQPKTTRSSPAKKYSDLMDHSISSYDLLPKALSPSTPSSRKNIHLLFSPKVPPRLSWTPPTSPKNTNKILSSELTRDEPSNPFIPARPKSAQASSSSNITRTHSVDLLGLYEQNHHHTPRPPITRSKSTTAIHHWEETDQTELSSIPSDEIQQQHQPKTTKSILKRPNSPSKGTRARFFSPRALSGPTGDDESDGNEVSWVDEHGNCRGREIIHEFQKDEPPMTRPKPILQSPQSPSPQKNTLPPPPSSSSSSTARRHHHTPKFIIPEPIDLGIEPILPRHSTSPSPSPPSLPPSSYLMNARAHRKSASTNTCHQVSRDPLQRTRSVSNSLIFNTPDNLSNILDESGGFLVGNETTWNGGDNNKERSTELSIDLNHVAMMELLGKKNLAKLRPGSVFAKLNATRGSECLMVPEEEESVDVSGLARQLEDKKSCPSDNLALTDEQAPPNLRGSMDFSTTFESSKINLPPLPTDREELWKNSQPKEQKKPPTTTRLAGVTRAKRYSMSSVDPQILGQRGGILDEETARLKKKIDSIKLSSQEDTRSVTKDDLFSELMSAPPPALGHQREKASSNRPISGQAKPTKPPPPPPSSERRPTAAKRLSAPVVVAFPGGKPQESKITSTNPVSKPRMSCISTSVVPVRAKRSSNIGIGLPASSSAPAVNALASKLSRPSTTGLTGVRSKLKPSNALVKPGLASGSPKTMKSRSSLSTITLNSPRPTYNPSPKKPSLLAQAPPTANTKMSSPTKIRKTLPVPGTTSTIPAPRPYTSIGSSARSSLLEPPSSRSALPQPASLGHRPRTSPFASNTLVSGISRPSSSSRIIPPASEPSKTLTARHIKQTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3L520</accession>
    <name>E3L520_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_17501</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ELP6 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178350">
        <property type="protein sequence ID" value="EFP91645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336064.1">
        <property type="nucleotide sequence ID" value="XM_003336016.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L520"/>
    <dbReference type="STRING" id="418459.E3L520"/>
    <dbReference type="GeneID" id="10531584"/>
    <dbReference type="KEGG" id="pgr:PGTG_17501"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17501"/>
    <dbReference type="eggNOG" id="ENOG502S92G">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1220192_0_0_1"/>
    <dbReference type="InParanoid" id="E3L520"/>
    <dbReference type="OrthoDB" id="9995306at2759"/>
    <dbReference type="UniPathway" id="UPA00988"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033588">
        <property type="term" value="C:elongator holoenzyme complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019519">
        <property type="entry name" value="Elp5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018627">
        <property type="entry name" value="ELP6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16184">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16184:SF6">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10483">
        <property type="entry name" value="Elong_Iki1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP91645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="227" mass="25965" checksum="BDB7235D0CB09334" modified="2011-01-11" version="1">MNFPLEDYSIPAKLSLLLVDSIDSPANFILLQLISNALRNHRAVILLLTDHDQNYWLSLLNKNRVNQLLLTIIQPKLDEDIKLLLTQQLTKSKNKNPLVIINNLSSIIWNNNLPTQQLTQSFKALLPSQLNHDFSLVYLFHNDLIKNNNNRDREIFIQLINSSDIILRTRALGGQAQTQGELIIQRGPRYLGDLNLPITTSSDRTVQFKIQDHSVSFHPKGLDKAFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3L5K9</accession>
    <name>E3L5K9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RNA polymerase II subunit A C-terminal domain phosphatase SSU72</fullName>
            <shortName evidence="9">CTD phosphatase SSU72</shortName>
            <ecNumber evidence="9">3.1.3.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_18028</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the cleavage and polyadenylation factor (CPF) complex, which plays a key role in polyadenylation-dependent pre-mRNA 3'-end formation and cooperates with cleavage factors including the CFIA complex and NAB4/CFIB. SSU72 is required for 3'-end formation of snoRNAs.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Processively dephosphorylates Ser-5 of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit (RPB1).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:20629"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate</text>
            <dbReference type="Rhea" id="RHEA:47004"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="EC" id="3.1.3.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Component of the cleavage and polyadenylation factor (CPF) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the SSU72 phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.16" evidence="9"/>
    <dbReference type="EMBL" id="DS178353">
        <property type="protein sequence ID" value="EFP91834.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336253.2">
        <property type="nucleotide sequence ID" value="XM_003336205.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L5K9"/>
    <dbReference type="FunCoup" id="E3L5K9">
        <property type="interactions" value="578"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L5K9"/>
    <dbReference type="GeneID" id="10531780"/>
    <dbReference type="KEGG" id="pgr:PGTG_18028"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18028"/>
    <dbReference type="HOGENOM" id="CLU_062463_0_0_1"/>
    <dbReference type="InParanoid" id="E3L5K9"/>
    <dbReference type="OrthoDB" id="57957at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005847">
        <property type="term" value="C:mRNA cleavage and polyadenylation specificity factor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008420">
        <property type="term" value="F:RNA polymerase II CTD heptapeptide repeat phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031124">
        <property type="term" value="P:mRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006369">
        <property type="term" value="P:termination of RNA polymerase II transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000182">
        <property type="entry name" value="RNA polymerase II subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2300:FF:000039">
        <property type="entry name" value="RNA polymerase II subunit A C-terminal domain phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006811">
        <property type="entry name" value="RNA_pol_II_suA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20383">
        <property type="entry name" value="RNA POLYMERASE II SUBUNIT A C-TERMINAL DOMAIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04722">
        <property type="entry name" value="Ssu72"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5 9" id="KW-0904">Protein phosphatase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="10">
        <location>
            <begin position="18"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="42"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU369031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP91834.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="267" mass="29069" checksum="9741800C364ED817" modified="2012-04-18" version="2">MSTNDPRLRARLAASNATPPPPAPGIPAKPSAVVVDHPALPPSVAADGSSSAAAAPSSAAQNGKLENERDIKLTCRNFTFCVVCASNQNRSMEGHNVLAREAFRVTSAGTGSMVRLPGPAIDKPNVYSFGTPYDDMYQDLKEKDERLYTANGILSMLDRNRKLKLAPERWQDSVVGVADVVITCEERCFDAVCDDLFSRGGELNKPVHLINIEIKDNHEEASLAGKALLDLCNAIEACKDLDSDLEEILEKQMEKHPHQLIHTVGYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>E3L6S5</accession>
    <name>E3L6S5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Pre-mRNA-processing factor 17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_18465</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178361">
        <property type="protein sequence ID" value="EFP92250.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336669.2">
        <property type="nucleotide sequence ID" value="XM_003336621.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L6S5"/>
    <dbReference type="FunCoup" id="E3L6S5">
        <property type="interactions" value="558"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L6S5"/>
    <dbReference type="GeneID" id="10537744"/>
    <dbReference type="KEGG" id="pgr:PGTG_18465"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18465"/>
    <dbReference type="eggNOG" id="KOG0282">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022571_2_1_1"/>
    <dbReference type="InParanoid" id="E3L6S5"/>
    <dbReference type="OrthoDB" id="10257301at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000034">
        <property type="entry name" value="Pre-mRNA-processing factor 17, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032847">
        <property type="entry name" value="PRPF17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43979">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43979:SF1">
        <property type="entry name" value="PRE-MRNA-PROCESSING FACTOR 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0747">Spliceosome</keyword>
    <keyword evidence="2 9" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="355"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="399"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="485"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="9">
        <location>
            <begin position="616"/>
            <end position="649"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="212"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="15"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="29"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="226"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="250"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="292"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP92250.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="649" mass="72949" checksum="75C3ED76CF72E985" modified="2012-04-18" version="2">MDGILAGYGSEEEVNQQTDDNTTAITTGLKEDQTRDESAAEAGSDPDDEIDNINGDVFGIKESLSNNKPKPEQSDQPSNSTDKQIITTVPHVIPAISSSTSLILRTSDTEMNVNVKYSDMILPKQGPKNPFTSRKLEKMNTLNGHIEEEFINDVDFNRQQRTFHVLKYAKNPSIISSTSENPALNFVGDVHAAYKNEGMLASEVKVTKAMKKTVKRKRQDKGELGVFDEEEEEAEEGEEGEQGPPKPPTKSREYKGPWAGWHDEHIEPVGPDEEEYEAAKRASTSASVVKKQATEKGQAKREVAYGEEKSTLHAKSLHDYQGRTYMHVPTDLDVDLLSTDPPSECFLPKRCIHTWMGHTKAVSAIRLFPESGHLLLSASMDSRVKLWDVYHDGKCLRTFMGHSKAVRDVTFSNDGKQFLSAGYDRQIKLWNTETGHCVQAFSNGKIPYCVKLHPDNDKQHIFLAGMSDKKIVQYDMRSGEITQEYDQHLGPVNTITFVDENRRFVTTSDDKTIRAWDFDIPVVIKYIAEPAMHSMPAVSLHPNNKWLAMQSLDNQVLIYSADSFKQNRKKRFAGHTIAGYACEVGFSPDGKFLSSGDGSGSMVFWDWKSCRILKRLKCHDQVIISHSWLPHETSKLVTASWDGLIKLWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3L891</accession>
    <name>E3L891_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178372">
        <property type="protein sequence ID" value="EFP92766.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337185.2">
        <property type="nucleotide sequence ID" value="XM_003337137.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L891"/>
    <dbReference type="GeneID" id="10539262"/>
    <dbReference type="KEGG" id="pgr:PGTG_18650"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18650"/>
    <dbReference type="HOGENOM" id="CLU_2050795_0_0_1"/>
    <dbReference type="InParanoid" id="E3L891"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92766.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="120" mass="12959" checksum="43C6FFA278A9E450" modified="2012-04-18" version="2">MLVTSNGSFQVLITAGTTSFQQFQEDILAKCNKRFEDSGGLIRNSMATHSPVFESGQDTNLATLEIVMQNPTTAVKKATKAMYVKSHLLTQEAAQKAGHPNKQVKAATIQLASSTPSMSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8K2</accession>
    <name>E3L8K2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA polymerase alpha subunit B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_18795</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DNA polymerase alpha subunit B family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178375">
        <property type="protein sequence ID" value="EFP92877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337296.1">
        <property type="nucleotide sequence ID" value="XM_003337248.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L8K2"/>
    <dbReference type="FunCoup" id="E3L8K2">
        <property type="interactions" value="172"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L8K2"/>
    <dbReference type="GeneID" id="10542684"/>
    <dbReference type="KEGG" id="pgr:PGTG_18795"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18795"/>
    <dbReference type="HOGENOM" id="CLU_1415804_0_0_1"/>
    <dbReference type="InParanoid" id="E3L8K2"/>
    <dbReference type="OMA" id="ARDIHHH"/>
    <dbReference type="OrthoDB" id="336885at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007185">
        <property type="entry name" value="DNA_pol_a/d/e_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016722">
        <property type="entry name" value="DNA_pol_alpha_bsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23061">
        <property type="entry name" value="DNA POLYMERASE 2 ALPHA 70 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23061:SF12">
        <property type="entry name" value="DNA POLYMERASE ALPHA SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04042">
        <property type="entry name" value="DNA_pol_E_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA polymerase alpha/delta/epsilon subunit B" evidence="6">
        <location>
            <begin position="4"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF04042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP92877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="192" mass="21241" checksum="E2F14742E6A7D88D" modified="2011-01-11" version="1">MPSTIFREKISSRLHKLVASSPRTHVILIPSPRDLLASQVDYPQAALNIKDPELGLFGRNIVCLPNPSIISINEVIIGINNVDVLMPLKKEEFFKQATVVVDEETAQEGTDPNATNVICRACRHVMRQRSFYPLFPHAIGIGMDAINLDVTHLDLLKHDTVGADILILPTNFTAFVKSIDSTVVVNPRHCAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3LA17</accession>
    <name>E3LA17_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19410</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178391">
        <property type="protein sequence ID" value="EFP93392.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337811.2">
        <property type="nucleotide sequence ID" value="XM_003337763.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LA17"/>
    <dbReference type="STRING" id="418459.E3LA17"/>
    <dbReference type="GeneID" id="10537259"/>
    <dbReference type="KEGG" id="pgr:PGTG_19410"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19410"/>
    <dbReference type="HOGENOM" id="CLU_1696371_0_0_1"/>
    <dbReference type="InParanoid" id="E3LA17"/>
    <dbReference type="OrthoDB" id="10389390at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93392.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="155" mass="16336" checksum="425993BED40C77DF" modified="2012-04-18" version="2">MAGPTTADQPVPPTSTTNTTCVIPGLSSQPPNNPASKRSHKKKTGSKVDNTTKAVARVPAFVDEPNELTEPAPAPLITSMPKPLNGLTPSAPPASASVSILPVQQQHIGGYESSTAPLNKDQKRAITSKPALEATVKELQDLLKILEEDEKLDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>E3LAH1</accession>
    <name>E3LAH1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">AAA+ ATPase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_19542</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178397">
        <property type="protein sequence ID" value="EFP93546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337965.1">
        <property type="nucleotide sequence ID" value="XM_003337917.2"/>
    </dbReference>
    <dbReference type="FunCoup" id="E3LAH1">
        <property type="interactions" value="545"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3LAH1"/>
    <dbReference type="GeneID" id="10543822"/>
    <dbReference type="KEGG" id="pgr:PGTG_19542"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19542"/>
    <dbReference type="eggNOG" id="KOG0732">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000536_5_1_1"/>
    <dbReference type="InParanoid" id="E3LAH1"/>
    <dbReference type="OMA" id="WPPSRHP"/>
    <dbReference type="OrthoDB" id="5421at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140674">
        <property type="term" value="F:ATP-dependent histone chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042393">
        <property type="term" value="F:histone binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006334">
        <property type="term" value="P:nucleosome assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006337">
        <property type="term" value="P:nucleosome disassembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045815">
        <property type="term" value="P:transcription initiation-coupled chromatin remodeling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001218">
        <property type="entry name" value="AAA family ATPase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000016">
        <property type="entry name" value="ATPase family AAA domain-containing protein 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000061">
        <property type="entry name" value="ATPase family, AAA domain-containing 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045199">
        <property type="entry name" value="ATAD2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036427">
        <property type="entry name" value="Bromodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23069">
        <property type="entry name" value="AAA DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23069:SF0">
        <property type="entry name" value="TAT-BINDING HOMOLOG 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47370">
        <property type="entry name" value="Bromodomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0103">Bromodomain</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="443"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="28"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="114"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="336"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="913"/>
            <end position="932"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="960"/>
            <end position="982"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1073"/>
            <end position="1107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1130"/>
            <end position="1190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1216"/>
            <end position="1262"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="117"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="199"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="219"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="230"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="265"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="918"/>
            <end position="927"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="1150"/>
            <end position="1161"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="1216"/>
            <end position="1243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP93546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1381" mass="154186" checksum="9041564426D9C70D" modified="2011-01-11" version="1">MPPDSHSPTTVLLLLTSTMNSSSISAAHQIDTVSSGRPSRNRKPPVTYNPHLPSTRRSKQPEPHHDDNHGIDPLVRTRRAHRVIGNIDNMGFNETHDESKELLNQSNSYSNHMLESEDKDADGEDDDGGDDDEYPSGKADSDSDYCAGNGEQSDPPAQSDDAITVKRSRRTQRVPIDSDDSFSQPRSTTRKARRKPVESDDSDEDEQEEIPSENEEFNDTGSIKQSTSSKDARTQKLEALARQRAIVAREQAGTRRSARNLTIRFGEDPHRNQSNKQAHEEDEGRQLRNRKHVDYHIPALDAFETEDKSKGKKERGRGIRLPMNMSGKQMDRLFAGQRPGADSDEEPTPAFGQVSGAGMLGGASAGPFGAPLLDPGSFAAGGPSNMGKMSGATNLADTDPLGVQTNIDFSHVGGMENHIQQLKEMVSLPLLYPEVFQRFQVTPPRGVLFHGPPGTGKTLLARALAASCSSEGQKISFFMRKGADCLSKWVGEAERQLRLLFEEAKNCQPSIIFFDEIDGLAPVRSSKQEQIHASIVSTLLSLMDGMDGRGQVVIIGATNRPDAVDPALRRPGRFDREFYFPLPNREARLSILNIHTRDWNPPPSEEFKSELADLTKGYGGADLRALCTEAALNAVQRKYPQIYKTSDRLVIDPQSIDVVPRDFTIAQKHLVPSTSRSTANIASPLPSHLKPLLERSFEQVKGLLDKMLPNVKKPNMLEEAEYENDDSGFEKEKTMQTFETLRIFRPRLVVCGSKGAGQQHIGAAILHHLEGYHIQSLDLANLIGDSTTSADARCVQIFTEAKRHKPSLLYIPSLHHWQSSPILESVTRTVTSLLDELKASDPILLLAIVDCPFKDLSKDIKSWFGVNRSNRVTLPSPDEAQRTEFFADTIANIRRPPTEFPDALPRKKRVLDVLPKAPPREPRKPTESELQSQLAQDQKLIEYLKFRLGPVLAELKKKHKRFTKPINPPPQQPDPQADEDEISKVPKYYDVDLEKMHYKLYYNEYLTSQQFVADVQKIVFNAEQEHQRSLSVDHETVVKAQAMLTHTRVMVEQACDHQFDIDCHRMYERMKLRNPLLGQPKKRTTPKEKNQPLPERQSSRVCGLEPEIPYDPLFSDRGVKRMISGCDAHTDHERPIKRNKGHQPSSSGSVKDDGAVDENHQTDGAIQESSAHVADVPDSTTPDYEPEPLKVMEPHLSGTIHSAVTLSLTNLFNVDNPQPNGLTHPQTSSTQGKTLNGDWNMQNGDPEPVSPQPQPNGNADVEMNASDQIPQHVNLGPDKDDPVGAPRPVEPPPPAIIPPIIYPDFVCPEQSVQAIHSELVYKTGRLSIDQLEELRAGCWNLIWNFRADFNRSLMIAECLSFIHQYFADILDDQELQNIDDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3NX60</accession>
    <name>E3NX60_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20019</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS989919">
        <property type="protein sequence ID" value="EFP94159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338578.1">
        <property type="nucleotide sequence ID" value="XM_003338530.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NX60"/>
    <dbReference type="STRING" id="418459.E3NX60"/>
    <dbReference type="GeneID" id="10535940"/>
    <dbReference type="KEGG" id="pgr:PGTG_20019"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20019"/>
    <dbReference type="HOGENOM" id="CLU_2886837_0_0_1"/>
    <dbReference type="InParanoid" id="E3NX60"/>
    <dbReference type="OrthoDB" id="2495624at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP94159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="64" mass="7497" checksum="CB7D3B3B6E3828D8" modified="2011-01-11" version="1">MSSDGQNWIDRIGPGSLFIVYALISLICLSPMFFPFYFKRRSIRLKASVWKPLKTSLSWNKDRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>E3NXE9</accession>
    <name>E3NXE9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SUN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20164</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS989933">
        <property type="protein sequence ID" value="EFP94248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338667.1">
        <property type="nucleotide sequence ID" value="XM_003338619.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NXE9"/>
    <dbReference type="STRING" id="418459.E3NXE9"/>
    <dbReference type="GeneID" id="10527335"/>
    <dbReference type="KEGG" id="pgr:PGTG_20164"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20164"/>
    <dbReference type="HOGENOM" id="CLU_1890800_0_0_1"/>
    <dbReference type="InParanoid" id="E3NXE9"/>
    <dbReference type="OrthoDB" id="2505323at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012919">
        <property type="entry name" value="SUN_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07738">
        <property type="entry name" value="Sad1_UNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUN" evidence="1">
        <location>
            <begin position="45"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP94248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="135" mass="14777" checksum="BB7CFA6236F94702" modified="2011-01-11" version="1" fragment="single">TNPKLRDLTTNKPSHLPYLLTLRAQPLQVSSIEADKDAYSIDGPNNVQEFSVARGELQLYSKVLLKIISNHGNPDLTCLYRVQVHDQLIHVDVTQDAMITAIIVVLNLISLGASGPLPTDKPLGPRSFSSTHISP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3NYB6</accession>
    <name>E3NYB6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20521</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP94565.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    <dbReference type="GeneID" id="10527941"/>
    <dbReference type="KEGG" id="pgr:PGTG_20521"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20521"/>
    <dbReference type="HOGENOM" id="CLU_046890_0_0_1"/>
    <dbReference type="InParanoid" id="E3NYB6"/>
    <dbReference type="OrthoDB" id="2505381at2759"/>
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    </dbReference>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="195"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="195"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP94565.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="432" mass="49105" checksum="1FF351C3180C3CBB" modified="2012-04-18" version="2">MDGTEVFSVAGLKRGSDYDGDRSLNSQAINFLNSQHQQSYQRVVDFDLHAVFLMQEFRAEDTIQQIITMIGTTFNEEYPPKNTLQQQLVNAAKLVNQRIQNYEQQPHMQKPFCLRFPTYSDVLKHCMELQDKCEVLPNDKMVILIDEGGVCVGVGLPPKPQSTQSIHTPRDPLSAGAADKTIKFATNSIKKIDLQGHGDHWKSDPKPNLPEPLRSDPGPTMRGLVELRHDMVFYHRISYWINKVFLPDASSIAESAVKYLEKKASTAVKESIQVEKNIIIAARTITVNTHPNTHRDRNNALLMDSVFFFGNHIGGHFLLPGLGVAYPGLHGYSFHGPFRILFHGVSQFHFTEDITDPRRYSVAMWSRASSFSEIARFSAHDNGNEKFSDDKYWLPLYPEYNPKITSDLLKAEVKIAKKEAKKESKKRQKTEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>H6QNT8</accession>
    <name>H6QNT8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20646</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EHS62526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890843.1">
        <property type="nucleotide sequence ID" value="XM_003890794.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QNT8"/>
    <dbReference type="GeneID" id="13542491"/>
    <dbReference type="KEGG" id="pgr:PGTG_20646"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20646"/>
    <dbReference type="HOGENOM" id="CLU_2868682_0_0_1"/>
    <dbReference type="InParanoid" id="H6QNT8"/>
    <dbReference type="OrthoDB" id="10273819at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="34"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="64" mass="7076" checksum="46E20522CEBFD0FE" modified="2012-04-18" version="1">MSLIDCLEQELYARSVPSAPTADFIKQAEARIELDQEGQRDPRTPSATEQSLSGQAPRRNCGLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>H6QP37</accession>
    <name>H6QP37_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20736</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EHS63151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890706.1">
        <property type="nucleotide sequence ID" value="XM_003890657.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QP37"/>
    <dbReference type="GeneID" id="13541410"/>
    <dbReference type="KEGG" id="pgr:PGTG_20736"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20736"/>
    <dbReference type="HOGENOM" id="CLU_2905209_0_0_1"/>
    <dbReference type="InParanoid" id="H6QP37"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="62" mass="6394" checksum="E7BDBBA045891B79" modified="2012-04-18" version="1">MSGEDDMETGSENEATNRPGGSTSVGAPGGGEESNDEEFDKILEGDGGDEDDDGKFLAFPSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPT5</accession>
    <name>H6QPT5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
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    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_20933</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EHS64322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890478.1">
        <property type="nucleotide sequence ID" value="XM_003890429.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPT5"/>
    <dbReference type="STRING" id="418459.H6QPT5"/>
    <dbReference type="GeneID" id="13542599"/>
    <dbReference type="KEGG" id="pgr:PGTG_20933"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20933"/>
    <dbReference type="HOGENOM" id="CLU_001324_0_3_1"/>
    <dbReference type="InParanoid" id="H6QPT5"/>
    <dbReference type="OrthoDB" id="2505996at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd18809">
        <property type="entry name" value="SF1_C_RecD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025476">
        <property type="entry name" value="Helitron_helicase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049163">
        <property type="entry name" value="Pif1-like_2B_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10492:SF57">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14214">
        <property type="entry name" value="Helitron_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21530">
        <property type="entry name" value="Pif1_2B_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helitron helicase-like" evidence="1">
        <location>
            <begin position="324"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="domain" description="DNA helicase Pif1-like 2B" evidence="2">
        <location>
            <begin position="1123"/>
            <end position="1167"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF14214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21530"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS64322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1250" mass="140679" checksum="EEAEB0197C09CF83" modified="2012-04-18" version="1">MLDIKALAVPTAADNTEQKILGAFIWKIPHRLGACTIECRSCGALHWMEESAIVDRKLLNISFSSCCQKDKVTLPVVDDSYPGYPLSLQSLFMGETEESKNFLDLIRMYNNSISFTLLGAKVDSTVQGQKGIYVFRINGGLTHRISSIEPVEGSEPAYAQIFVVGEGGTEEAELRMNKAQGKGGARGRGSRLCVKNIKKLVDFLDSYNPYAVLYRCAQEVLQQTNAKTLALQGVQHPSGDPKRYNQPSVDEIAVVIHGDGDTIGERQIVLHRNDGLLDIISDVHSSYFPLRYPLFFPYGSQQWDNLYQAWTGRVNGRKVGSLEWFAFLLFERKEDFSPILAGRSLFQEFLVDMYVVVERSRLRFITFNQGKLKAAQYDKLISLIDGVAPSCGRRIILPSSFIGSPRYMRQLYQDSMAICRKYGPPSLFITMTANPRWKEIQDQLKPGQKAYDNPTVVARVFHLKSRELLHQLVKLNRLGKVVAYVLTIEFQKRGLPHIHLMLTLDEKDRPITPEKIDLLVNAEIPNKDDEPKLHSLISDFMLHGPCNGRACWTEKGCRLGFPKPLTPRTVTVDGTYPIYRQRENGPTIQKFTTQFDNGSVVPFNKYLSLMFECHINVEVPVSSSAIKYLYKYITKGHDRSYLKIQEEDETKAFVDARYISPPEAAWRLLKFSLSERYPAVTRLALHEDNEQLVYFDSEESAVGQIKSGSAENTMLTEFFRLNAENGRGADNQSARTLFYEDIPAYFSWSRSEKKWKPRKTKSEAVGRIYGISYLAGEKFYMRTLLLHRKNIVSHEDLKTVGNRIAGSYQEACNWLGLLVDDTLYHATLNEASFVRSGFQLSQLFAMICVHTPPSAPMQLFEDHYENMTDDIVRVDMKFRDSETLTLDERRLLGLARIDSMLHELGSNLQRCGLVTSAEESSFLMNIHGNEPIIESIELIRDRLVANLSRFNEDQLYIYTQVKRCLLARKGQLFYIDGPGVVASSGVAALLLKGAAVWREGPTGKEAKRNRAFARALLGIGEGRTNTQENDVIKPKHLHIMSFSTPQESRKALINFVYEHLATYAGRPPSENADYLQDRCILAPLIKDVAILNDEITAILPGIGFMSKSIDIPDPEGWNSLPEECLNTLSPPGLPEHRILLKVGMPIVIIRNLYIARGVCNGSRMIVKEVGEGFILVRPAYAMSINKSQGQTLACVGVLLETDCFAQGQLYVALSRVSNRRAACNLEGLAGGGEGYEGLALPLPQNEYQSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQG4</accession>
    <name>H6QQG4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">40S ribosomal protein mrp2, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_21123</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS14 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EHS62577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890290.1">
        <property type="nucleotide sequence ID" value="XM_003890241.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQG4"/>
    <dbReference type="FunCoup" id="H6QQG4">
        <property type="interactions" value="184"/>
    </dbReference>
    <dbReference type="STRING" id="418459.H6QQG4"/>
    <dbReference type="GeneID" id="13541168"/>
    <dbReference type="KEGG" id="pgr:PGTG_21123"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21123"/>
    <dbReference type="eggNOG" id="KOG1741">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_139869_2_1_1"/>
    <dbReference type="InParanoid" id="H6QQG4"/>
    <dbReference type="OrthoDB" id="413436at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1480:FF:000001">
        <property type="entry name" value="30S ribosomal protein S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1480">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001209">
        <property type="entry name" value="Ribosomal_uS14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19836">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19836:SF19">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US14M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00253">
        <property type="entry name" value="Ribosomal_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS62577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="103" mass="11957" checksum="626DA019A226094C" modified="2012-04-18" version="1">MRSPINIRTLRDMTRRDQFASTELLRRAYLYLARNEGLPMKLRAQAQLSLNNLPSNSHPTKIHERCIITGKGRGVIAKLGLCRHQLKLKATRDKWMGFEKAVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQL3</accession>
    <name>H6QQL3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21173</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EHS62665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890211.1">
        <property type="nucleotide sequence ID" value="XM_003890162.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQL3"/>
    <dbReference type="GeneID" id="13541264"/>
    <dbReference type="KEGG" id="pgr:PGTG_21173"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21173"/>
    <dbReference type="HOGENOM" id="CLU_2039205_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQL3"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS62665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="101" mass="11077" checksum="D967B75FE3EF59CF" modified="2012-04-18" version="1">MSFSRPLSIASLDATITGTCSESDSIDERSPIDFDSVAICSPVDYDSVDKRSPAQPGDYSNFSPAFTQDTFLLEELHDFRILITDELICFARSASAANEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRV4</accession>
    <name>H6QRV4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21472</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EHS63396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889765.1">
        <property type="nucleotide sequence ID" value="XM_003889716.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRV4"/>
    <dbReference type="GeneID" id="13543087"/>
    <dbReference type="KEGG" id="pgr:PGTG_21472"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21472"/>
    <dbReference type="HOGENOM" id="CLU_2850763_0_0_1"/>
    <dbReference type="InParanoid" id="H6QRV4"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="65" mass="7171" checksum="6AD40F1AA4B42AE5" modified="2012-04-18" version="1">MLIADQGFRLKVPIGTANALQQTPSAHLKGEVPMEEGCNSSISGEGVRRYQNAEEGRNWLPIRTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSB9</accession>
    <name>H6QSB9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Prolyl-tRNA synthetase</fullName>
            <ecNumber evidence="6">3.1.3.36</ecNumber>
            <ecNumber evidence="5">6.1.1.15</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PGTG_21656</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="21" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>tRNA(Pro) + L-proline + ATP = L-prolyl-tRNA(Pro) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14305"/>
            <dbReference type="Rhea" id="RHEA-COMP:9700"/>
            <dbReference type="Rhea" id="RHEA-COMP:9702"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78532"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.15"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the synaptojanin family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the central section; belongs to the inositol 1,4,5-trisphosphate 5-phosphatase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.36" evidence="6"/>
    <dbReference type="EC" id="6.1.1.15" evidence="5"/>
    <dbReference type="EMBL" id="DS178292">
        <property type="protein sequence ID" value="EHS63647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889583.1">
        <property type="nucleotide sequence ID" value="XM_003889534.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="H6QSB9">
        <property type="interactions" value="218"/>
    </dbReference>
    <dbReference type="STRING" id="418459.H6QSB9"/>
    <dbReference type="GeneID" id="13542659"/>
    <dbReference type="KEGG" id="pgr:PGTG_21656"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21656"/>
    <dbReference type="eggNOG" id="KOG0566">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003016_2_1_1"/>
    <dbReference type="InParanoid" id="H6QSB9"/>
    <dbReference type="OrthoDB" id="405996at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052658">
        <property type="term" value="F:inositol-1,4,5-trisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043813">
        <property type="term" value="F:phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004439">
        <property type="term" value="F:phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004827">
        <property type="term" value="F:proline-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046856">
        <property type="term" value="P:phosphatidylinositol dephosphorylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006433">
        <property type="term" value="P:prolyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.10.10:FF:000029">
        <property type="entry name" value="Inositol polyphosphate 5-phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.10.10">
        <property type="entry name" value="Endonuclease/exonuclease/phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036691">
        <property type="entry name" value="Endo/exonu/phosph_ase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046985">
        <property type="entry name" value="IP5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000300">
        <property type="entry name" value="IPPc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002316">
        <property type="entry name" value="Pro-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002013">
        <property type="entry name" value="SAC_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200">
        <property type="entry name" value="INOSITOL 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11200:SF257">
        <property type="entry name" value="PHOSPHOINOSITIDE 5-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22669">
        <property type="entry name" value="Exo_endo_phos2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02383">
        <property type="entry name" value="Syja_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01046">
        <property type="entry name" value="TRNASYNTHPRO"/>
    </dbReference>
    <dbReference type="SMART" id="SM00128">
        <property type="entry name" value="IPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56219">
        <property type="entry name" value="DNase I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50275">
        <property type="entry name" value="SAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0436">Ligase</keyword>
    <keyword evidence="10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-0653">Protein transport</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="SAC" evidence="18">
        <location>
            <begin position="142"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="19">
        <location>
            <begin position="1359"/>
            <end position="1626"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="951"/>
            <end position="1224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1312"/>
            <end position="1335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="981"/>
            <end position="995"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="17">
        <location>
            <begin position="1077"/>
            <end position="1086"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1089"/>
            <end position="1108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="1143"/>
            <end position="1167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00029731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00047671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="EHS63647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1688" mass="185723" checksum="2A74AABF4548E91B" modified="2012-04-18" version="1">MKRAELSLGLILKPRSFVISSSTHALLLRPFDRSPSKQLIVEFVEVDETTETGIKLAPCWGCLGILEIEREIFIVIVTHATPIGILQPSHEQIHRVVSVDLFCLTSAAFDYQHIFHHPTGNINSSIDFDEHAAPDHPALSGIKKILSNGQFYFSTTVTDLSSRTEHRVTAENRSEDDSYRRFSWNSFMLVGLSGFQHGLPIESRLQFDRQNFLVSVIQGFVATYELKLPPPPPSTANDDPLNDQLNEPISFSISLFSRLGSRRAGTRFNTRGIDDDGNVANFVESETLIVSGSNLFSFVQIRGSVPLFFEQSGTGMTNVIGGLSGQGHRTQLTRTGIATQPAFERHFEDLLQQYPSVHIINLLSNSREGECSLSQAYQQRLKVLARDEVQMAHFDLHQRAKAGGLDGVRQQLKNDLMIGGQIEQMGSFLASMSPEGQIEVVTRQDGVFRVNCLDCLDRTNVIQEMLSRMAVEAFLSATHPHLLRSDYLWNCHRTLWADNGDVLSKIYAGTGALNTSFTRGGKQTFSGILSNASKSVGRMYNSNFIDKSKQAHIDLMLGNLSGQKPVKVFDPLNDTIKKMLHDRRSDYSQTLPISLWVGTYNLNGRAPPMANNDELMAWLCPTSNFEPDIMAVSFQEIVKLSPQQIMITDPEKKNRWERVILTSLENRPNKKSSYAIIRSDQLVGAALIILAKTELVPAVRSVEAKTKKTGLKGIAGNKGAVAIRLDIHDSSFCFVTAHFAAGLSNVEERNHDYETIEDGLKFLRGKTIASHENVIWAGDFNYRIDGGLQNNAVRQAVVDENYAELLAADQLLEHMAVQAVFPKYTEGAINFPPTYKYDVGTDRYDTSEKMRIPAWTDRILYQGSDLNLIDYSRAELKASDHRPVYATLETKVRMVDRVKKEVIKQEILESSEVGSLRDALMMPLQAGDSSSLEATVLDEWWKTPEHPNGACELNPPEQAQGIGGSSNPFIPNIGPPRPGRQCSTPEANPVGSNRLFNGKLDRQISSSVPSSPPPQVGKGIPPAIPARPASRSPSETRKPVTEKSGSTLKAQPPPPPPRPTSKLIVSKDNTLVEIIPKGPPRPPPTTPGSLLTSQISPTSTETFNSRARSLSGRKSAPSGLQLGVPPPPSHPALKLPHRQESVKSPLLSASTSKLTSSKQVTPIRPGYTTPPPPPRPTSRGFANTADAPIGVENPQFSPRSTLKLPQRRETTPAELDSNNPPLPPRPVSRLLTSEETPLVSGVEPKGFVSLNVLAGKGIDSAELDRKNPTPPPRPASRIVFGKELATNGGGIGTEGPSLPARPVSTLLTAGNPYSPTIKLDQNQRNKLTGDPESGEGDASVLVRAGFVRQSSSGIWSFLPNGLRVLNKIQAIISREMERIGASEISMPLLQSQKLWDRSGRSNQAGPELFRLKDRKGSSLLLSPTHEEEVTNLVKSDIQSGKQLPIKLFHIGRKFRDEARPRAGLLRSREFIMKDLYTFDADASAAMASYKEVQEAYRRIFETIGIPFSVAEADSGAIGGATSHEYHYKSSSGEDSLIHCTRCPYTANLELARSTLSPRVEDPSQLQVQFFSSPSSRRFLAAVLPATHELHPLKLSKACPDIRPVQSANEDVLPAFETEWDQMEVLVDESCLAIELEQIFERLARAIPTLDLAQPTDLHCPRPTHGVRRGPRVEHAELVRRACPDCGSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTU9</accession>
    <name>H6QTU9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_22179</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178322">
        <property type="protein sequence ID" value="EHS64355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889065.1">
        <property type="nucleotide sequence ID" value="XM_003889016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTU9"/>
    <dbReference type="GeneID" id="13541788"/>
    <dbReference type="KEGG" id="pgr:PGTG_22179"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22179"/>
    <dbReference type="HOGENOM" id="CLU_003292_5_0_1"/>
    <dbReference type="InParanoid" id="H6QTU9"/>
    <dbReference type="OrthoDB" id="3266428at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011010">
        <property type="entry name" value="DNA_brk_join_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013762">
        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010998">
        <property type="entry name" value="Integrase_recombinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052925">
        <property type="entry name" value="Phage_Integrase-like_Recomb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34605:SF3">
        <property type="entry name" value="P CELL-TYPE AGGLUTINATION PROTEIN MAP4-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34605">
        <property type="entry name" value="PHAGE_INTEGRASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56349">
        <property type="entry name" value="DNA breaking-rejoining enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47823">
        <property type="entry name" value="lambda integrase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS64355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="317" mass="34730" checksum="EAC83741B194B80D" modified="2012-04-18" version="1">MPCIKQLDSLSSIIDFTSNGLVLKTPTALDRHMLNGWSPSTLQSYNSAVRKFLQFKRSCGHYDFQLPATEDDIYGFCFWAGKKDKDTSGSGVLTVTLKKYLQGLKAWHLFHNAPYPAASAAKVAVLLKASAKVDVKQPKTPQKRPVMIHHLVILANELSGGDEADLAILDLALVTFWSLARLGEVTRDPKKGNTQGILPGDVKINNDYGSATIELKESKTARPGESQFLRLRSMPNLLCPIKALQRRLAAADKGEQLFSFVVKGNRTDLTKYLVRKRLAEVWGGCGFHGLSGHSFRVGGASFLKALGVSTARICEIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>H6QU63</accession>
    <name>H6QU63_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tc1-like transposase DDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22314</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178331">
        <property type="protein sequence ID" value="EHS64526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888931.1">
        <property type="nucleotide sequence ID" value="XM_003888882.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QU63"/>
    <dbReference type="GeneID" id="13541135"/>
    <dbReference type="KEGG" id="pgr:PGTG_22314"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22314"/>
    <dbReference type="eggNOG" id="ENOG502S8Y2">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056788_1_0_1"/>
    <dbReference type="InParanoid" id="H6QU63"/>
    <dbReference type="OrthoDB" id="2505980at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047655">
        <property type="entry name" value="Transpos_IS630-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033545">
        <property type="entry name" value="transpos_IS630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564:SF1">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="1">
        <location>
            <begin position="151"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="338" mass="39423" checksum="86A3ACBA3E00F6BA" modified="2012-04-18" version="1">MPYFYYPPNVKVMAVCMTLEGKTRDNIRQALGFQISKQSFNRWREHYEQTRRVIRNPEEYERLGRPRTLTSEDCTFMLQLVRNEPGLFLDEIREKVYDATGTLLSVEAVHENLVNNLLITLKKAETLNSRKCLLKKYRYVARMSFYPANYLVFTDESAICDRALLRTHSRSPCGTPAARYIIRQNPERFSILPAISINGIIALKVRDDTYTGIKFEHFLKWTLLPRMNKYPSPNSVLVCDNARIHRGRYIEAICDKFGVRVIYLPPYCLELNPIELCFANVKSHLRRTQALSVTDQPEALIHRACVKVMTASLCHKLYRHCGYVVPSLEHPSRPRRFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUA4</accession>
    <name>H6QUA4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22359</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178334">
        <property type="protein sequence ID" value="EHS64567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888903.1">
        <property type="nucleotide sequence ID" value="XM_003888854.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUA4"/>
    <dbReference type="STRING" id="418459.H6QUA4"/>
    <dbReference type="GeneID" id="13542375"/>
    <dbReference type="KEGG" id="pgr:PGTG_22359"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22359"/>
    <dbReference type="HOGENOM" id="CLU_739959_0_0_1"/>
    <dbReference type="InParanoid" id="H6QUA4"/>
    <dbReference type="OrthoDB" id="2507294at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="101"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="203"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="374" mass="43264" checksum="14D1CF4A27BF8E70" modified="2012-04-18" version="1">MQTVNVDKNTGISCDCEFNKLTIKEDIVECCTLLTSEVTTRLESYKKELSAIVDVKDKKLVQVVQELSARIESLSKGIKAITPDSMEKTGDSVHIKKNENPRIQNGNRETPTLPDRDPSHNDVMLFGISHEIKQLAEAVRSKNESCVTKSSVEQMIRDLSEEHHRDFSSLSTKLDQLLSQFRDDGAEVRASIGEINDRMDTSPVRSSGQAAQPGSNFRRRNDQYSIQPTSSNNFRNTQPTNLQSVGISEMQKQLWAAVNKLDWPKFSGQGEYDHTEFIDWIDDCRDETSIPDEYIQVKLLSILTGVASQWYKSMRKDHKDEKWDFWREEIRKQYGTSNWMRKKQESFEKDRFETRVLRERQIYTWRNTSIVLGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUE7</accession>
    <name>H6QUE7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22398</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178337">
        <property type="protein sequence ID" value="EHS64610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888870.1">
        <property type="nucleotide sequence ID" value="XM_003888821.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUE7"/>
    <dbReference type="GeneID" id="13542420"/>
    <dbReference type="KEGG" id="pgr:PGTG_22398"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22398"/>
    <dbReference type="HOGENOM" id="CLU_003292_8_2_1"/>
    <dbReference type="InParanoid" id="H6QUE7"/>
    <dbReference type="OrthoDB" id="3254696at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09275">
        <property type="entry name" value="RNase_HI_RT_DIRS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052055">
        <property type="entry name" value="Hepadnavirus_pol/RT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050:SF7">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="535" mass="61457" checksum="AD943443B81C0B23" modified="2012-04-18" version="1">MNVDEWERALEKAGLAQEFSDVIRGFKEGFDQGIPNHNLGPATPYFTPPNHQSALLAQDKIEQSMKKEVEAGRMFGPYTHEQLMKKFSFFRTNPLGAAVNGDGSIRPINDLSFPRNNPLTPSVNSFVDKLDYATTWDDFENVSKFFRRQTGPLLLALFDWEKAYRQIPTAKSQWAYLMVRDFNGGILIDTRIAFGGVAGCGSFGRPADAWKQLMLHEFDLVTVFRWVDDNLFVKHPDSKVEMDHIVARSESLGVKTNSTKYSPFKEEQKYIGFIWNATRKTVRLPDDKKYQRVQQIKEFLQTGSEFSFKQVEVMAGRLNHVSYLLPQLRCYLNSLYRWMNAWVYRSKDLPLPPGARVDLQEWLTILLSFKETRMIRDPDPIEIGWMGDASTSYGIGITIGRRWAQFQLTKDWDRGPEPKRDIAWLETVAIRLGLIALAQLSVKRGKTIIVWTDNTTTESVILKRKSKHQAVNDEWKIIQKLLVEMELDIVSRRVSSGDNVADALSRGNREGRNPQLQIPITVPLDLEHRMFQSVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>H6QV00</accession>
    <name>H6QV00_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DEIH-box ATPase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_22588</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178366">
        <property type="protein sequence ID" value="EHS62613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888661.1">
        <property type="nucleotide sequence ID" value="XM_003888612.1"/>
    </dbReference>
    <dbReference type="FunCoup" id="H6QV00">
        <property type="interactions" value="949"/>
    </dbReference>
    <dbReference type="STRING" id="418459.H6QV00"/>
    <dbReference type="GeneID" id="13542259"/>
    <dbReference type="KEGG" id="pgr:PGTG_22588"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22588"/>
    <dbReference type="HOGENOM" id="CLU_000335_2_0_1"/>
    <dbReference type="InParanoid" id="H6QV00"/>
    <dbReference type="OrthoDB" id="5575at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18021">
        <property type="entry name" value="DEXHc_Brr2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00174">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000012">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000024">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000004">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000001">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:004793">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001452">
        <property type="entry name" value="SH3_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057842">
        <property type="entry name" value="WH_MER3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF4">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00018">
        <property type="entry name" value="SH3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23445">
        <property type="entry name" value="WHD_SNRNP200"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00326">
        <property type="entry name" value="SH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0728">SH3 domain</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="97"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="8">
        <location>
            <begin position="282"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="884"/>
            <end position="1068"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1451"/>
            <end position="1474"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS62613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1474" mass="167041" checksum="53E69D61CF50D4DC" modified="2012-04-18" version="1">MSKPEQPSKLVTIVYAIHHFQAENPDELSFSAGKPIEVTEKNDLYGDGWWQWYPCSFRRPYLSHTEDLVKIVDLPHWSREAFKGATTLNRVQSKVFPVAFGQDDPILLCAPTGAAKTNVAMPTVLNEIAKHRNEATGGIDLAAFKIVYVAPMKALVQEMVGNFSSRLEYLGIQVGELTVGLIIIDEIHLLHDERGPVLEALVSRTIRRMEQNHEYVRLVGLSATLPNYADVARFLRVNPKKGLFFFDSSARPCPLKLEFIGITEKKAIKRLQLTNEICYEKVMKQLNDKQQIIIFVHSRSETTRTAKNLKETSIERDEVGKFMSGGLATREILMETAENVKDPGLKDILQFGIGIHHAGLPKTSKTRGSRKFGIGIHHAGLERVDRRLVEELFADGHLQVLVSTATLAWGVNLPAHAVIIKGTQIYNPEKGRWVELSPQDILQMLGRAGRPQYDTFGEGIIITNHSELQFHLSITTSQLPIESQLVSKLADILNAEIVLGTIRNREEAPQWLGYRYWYQRALENPSLYGFQHDPEDPLLLQKRTHIVHTAICILEKSGLAKYDRKTGLIATLELGKIASHYYVTNTSMSTYNQHLRPTMTLIELFRVFAASDEFKYIPTRPEEKQELAKLLEKVPIPVKESVGDPSAKINVLLQAYISRLPLEGFALMADMVYVTQSAGRILRALFEICLKRGWARLTHQVLDLCKMVEKKMWVSMTPLRQFPSCSADIIRRAERKDFPWYRFFDLEPPELGELMGNPKLGKTIHRFVHQFPKLELQALVQPITRTMLRVELTITPDFMWEESVHGTAQTFWIMVEDVDGELILFSDQFLLRQRYANEEHFVTFDVPMIDVADRWLHAVLVYRCPSSISSYPKSSPSLLRYFFQALYTGNDNVLICSPTGSGKTTCAEFALLRLWSQPEWQRCVCIEPYQEVVDLRVKEWRQKFGPLGKVIEPLTGELTRDVELTASDGSKPGQARIDIIICTPTQWDLVSIRWKQRKMVEGTGLLIADEIHLIGSEIGPAYEVIVSRTRYVTAQSEISKTRIVALGCPLANARDLGDWMGANSQAIFNFAPGSRPLPLEVHIQSFNVPHFPSLMIQMAKPAYLSILEYAHEKPVIAFVPSRKQCRLTASDLSIYALSDEDPQRFLNIEQEDLAPYLAKVSDENLRETLASGIGYYHEAMSNTDQVIVQKLFEEGAIQVVIASKDTAWKIVAKTIENKQDAVDWCTWQWFYRRLVADPNYYNMQATDHRHLSDHLSELVESTLSDLQNSNCIAIEDEMDTTPLPLGPMSLTEKTKLKGILEIISAAQEFESIPLRHGEEGLLKKVHDRVPVKVGKVEYLSPHFKTNILLQAHFSRLTLPSDLMLDQVEILRKVPNLISAAVDVLSSQECLNTTVAMEFFQMVVQAVWNHDSPLKQIPGFSSEIIQRCTAANVNQVTDIMESVTIPIQEAQKMFPPPRQPSPLSRARVQKAQTGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQ55</accession>
    <name>E3JQ55_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Deacetylase sirtuin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_00094</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sirtuin family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="GeneID" id="10527861"/>
    <dbReference type="KEGG" id="pgr:PGTG_00094"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00094"/>
    <dbReference type="eggNOG" id="KOG2684">
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    <dbReference type="InParanoid" id="E3JQ55"/>
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        <property type="project" value="GOC"/>
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
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    </dbReference>
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        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF15">
        <property type="entry name" value="NAD-DEPENDENT HISTONE DEACETYLASE HST4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="7">
        <location>
            <begin position="29"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
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            <begin position="160"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
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            <end position="436"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
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            <end position="572"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
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            <end position="641"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="176"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="202"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="218"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
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            <begin position="233"/>
            <end position="255"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="6">
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    </feature>
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    </feature>
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        <location>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006924"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
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    </evidence>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP74138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="641" mass="68850" checksum="C2963ADAEE16D37A" modified="2011-01-11" version="1">MSPPTQLVVLPGTRQTALGPLPSFVKPVSDSPEPDLQRAIKAVLNAKKVAVVVGAGISTAANIPDFRSSTGLFASLKARFPNAKLTSGRDLFDVNAWKDPDTLSLHFGMLAELHKMCGAAQPTAFHQFLKRLDDDGKLSRVYTQNIDGLEEKVGLTYGIPGQDEIQSHSSQTSRSRRSDPPSTPRERNLKRKRDENGSAQLPPTPRSTPPPSQRSESEPSNSSNSQSELDEPGSSQLSTVSTITSSDQKPSSLDTPKSKPRTRAVSAAEAAEAPAPPRPTRSKPVDPFPRVIPLHGTLKNLSCLTCKHSVLMSDYIGELAEGEPILCPQCQSFDNARAISGFRTRGVGLLKSGVVLYGEEHNAGDQVGAVTSRDLMRGRRPDLLIVAGTSLKVPGTKRLVKELSKVIRPPTKTSSVSGDEEQEEEESSSSSKKPAPIHTIFLNNEFPSPCGIWKETFDVWVKGNVDQFIQLVDEERKVQEELKTKKAQEKLERERKKEEKLRRGAGSAASQPSSSSASSSAVVPEPSASAPVTSKARTTTTAPATKPKKLILKVSPQSTSAKSAQPKPSVAGYFKTGKASVVASKPVVKINNLKNPTTIKISNKLLSAPPAPAGKPTPAPKSTPATRKKKSNPPRRNVKCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQM2</accession>
    <name>E3JQM2_PUCGT</name>
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        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_00190</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP74234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003307192.2"/>
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    <dbReference type="STRING" id="418459.E3JQM2"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00190"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR002110">
        <property type="entry name" value="Ankyrin_rpt"/>
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    <dbReference type="InterPro" id="IPR036770">
        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050745">
        <property type="entry name" value="Multifunctional_regulatory"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24189:SF50">
        <property type="entry name" value="ANKYRIN REPEAT AND SOCS BOX PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24189">
        <property type="entry name" value="MYOTROPHIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12796">
        <property type="entry name" value="Ank_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="50"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="84"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="161"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="4">
        <location>
            <begin position="168"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP74234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="194" mass="21417" checksum="7F03CBCA41C1DBE0" modified="2011-01-11" version="1">MGDLPDSGTPQDKGAAPNERLLFAAKQNSEDLFKEVEENGGYDINYQDNLGNTALHYAVIRQSPHVMELILEHEGADVDLRNKEGKTPLLCAVEIDHPVAMFEIMRSLLDAGADTRLRDKRTKESLTDKLRQRIRTRRQVSEGAPGSGDEEARLMRIIEEAEAAGQIDQDDIASEDDSSGPRDAPSDDEDVASD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3JT84</accession>
    <name>E3JT84_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_01956</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319782.1">
        <property type="nucleotide sequence ID" value="XM_003319734.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JT84"/>
    <dbReference type="GeneID" id="10542910"/>
    <dbReference type="KEGG" id="pgr:PGTG_01956"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01956"/>
    <dbReference type="HOGENOM" id="CLU_570029_0_0_1"/>
    <dbReference type="InParanoid" id="E3JT84"/>
    <dbReference type="OMA" id="HEMRDQR"/>
    <dbReference type="OrthoDB" id="3338772at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="17"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP75363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="479" mass="54923" checksum="F9A280D1ADB1948C" modified="2011-01-11" version="1">MSSSTYHPLPANESQLPLVSPPSTHHHHHHPARFDSHPSRSRLKFFVLRVILPLLSLLLLLTLVIIPATLSNQFSFKQCQDGSCLKWDKVHDLAQNWGLGFQKLSPTAGPIRSKLKILHLVDKDTAEVLMDRWLLHSHDAFLARPDLVSSAILWGPDFEGWKNTLSLTQNIAQKFGSADHFDAVLVYFNNDQVKFLHIDSKPWFAQVKELSQQGNGVVIIDRPHEMRDLRKAEFYNLANVTLVLAPYAYELFEYRSQFATQALLVHQPHFIDKKIFYHPVGNTRPEDIIIAGDLGAFYPFRARLADMIRKKTLPGWIRGHPGYEDIKSADQRNKKLTAEIQEQQLVDFGNDFGRAKICLVTDSRWAYSVQKYVEAAAAGCLIAGNIPLDRQAMFEKVIVPLSNKDSDDKIITTLNWWLKHDQERISKAQAAQDWILNSFSMDKYAEDVWKMIWFVKEGQRGMILPYEFDFLPKDLPEDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3JTN0</accession>
    <name>E3JTN0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01998</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75405.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319824.2">
        <property type="nucleotide sequence ID" value="XM_003319776.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JTN0"/>
    <dbReference type="GeneID" id="10542952"/>
    <dbReference type="KEGG" id="pgr:PGTG_01998"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01998"/>
    <dbReference type="HOGENOM" id="CLU_2470173_0_0_1"/>
    <dbReference type="InParanoid" id="E3JTN0"/>
    <dbReference type="OrthoDB" id="10404561at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="26"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75405.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="88" mass="10114" checksum="ADD1DC2DA99AE4B2" modified="2012-04-18" version="2">MSIYEGFEIHLSLVHIRKTDYLFARTKPTPSKSDRPTFNFRDQNQHHRRLQGDQQASKAINRLPRRSTGGYPRLIDCALISAMDGASK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3JTN2</accession>
    <name>E3JTN2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02000</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75407.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319826.2">
        <property type="nucleotide sequence ID" value="XM_003319778.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JTN2"/>
    <dbReference type="STRING" id="418459.E3JTN2"/>
    <dbReference type="GeneID" id="10542954"/>
    <dbReference type="KEGG" id="pgr:PGTG_02000"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02000"/>
    <dbReference type="HOGENOM" id="CLU_013347_0_1_1"/>
    <dbReference type="InParanoid" id="E3JTN2"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053094">
        <property type="entry name" value="Zinc_metalloprotease_ZmpB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193:SF2">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="306"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="324"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="347"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="397"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="439"/>
            <end position="450"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="451"/>
            <end position="466"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75407.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="602" mass="67803" checksum="6ECD9D769ED6D911" modified="2012-04-18" version="2">MYTTSDSEERTGPFHSSELEEGKWYDKHLPMANPNKEESMEEEEDTGVQEMWENGFIAVANGEWMNKQQFNSAIQDYPDLMFNRDIPIQFGRMANQMKTQPAHTQIQRAVSGLSRPKPPAHARADRSSEPQLASSIQPESTGIPKPQETQETKDPLNDVEPPAKIYPESLAHVTPHPSRKLSRMPSTTTTIGAALGIPSLAKASLEEMKDMHSIWSRTRDHLCKSMEELRIGGHHIKTPSAYDNLASHNSLVDIFCRRLQGRIQDAEKSGISKEIEVVDLIDLTGDDNDYPNSCLGERRLKRASRLSDIEDEDESPVQTKRARNSSQQIILSEFTSNDGPPKIITSKPPQDNNQSEAASHPKTTEFTVLYQNPRPETRPEPPRPMTRSSTRSQNTKSSKDVQPPKEKSISTKEPSALPNEPSPLPNEPSTESKEATSTKGKKKAGGRKNKKQEETPEARKQNQEERKRKREEKKKSPYNDPPQPGTLLTKIRKKSESVRMHNLYSTMLEMSRNPQWDYGPELMELVADMVQWSAGDTECDEFYEMPELFTPSVEAIQALGVAHDPDFRHCISSKTHPKPQSQTPGYCSWALEIHSSSGKSST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>E3JU26</accession>
    <name>E3JU26_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNA helicase</fullName>
            <ecNumber evidence="3">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">PGTG_00882</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="17" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">ATP-dependent RNA helicase required for 60S ribosomal subunit synthesis. Involved in efficient pre-rRNA processing, predominantly at site A3, which is necessary for the normal formation of 25S and 5.8S rRNAs.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="3"/>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75551.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319970.2">
        <property type="nucleotide sequence ID" value="XM_003319922.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JU26"/>
    <dbReference type="FunCoup" id="E3JU26">
        <property type="interactions" value="193"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JU26"/>
    <dbReference type="GeneID" id="10543267"/>
    <dbReference type="KEGG" id="pgr:PGTG_00882"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00882"/>
    <dbReference type="eggNOG" id="KOG0331">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_1_5_1"/>
    <dbReference type="InParanoid" id="E3JU26"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000464">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00268">
        <property type="entry name" value="DEADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000008">
        <property type="entry name" value="ATP-dependent RNA helicase RhlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044742">
        <property type="entry name" value="DEAD/DEAH_RhlB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0347">Helicase</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5" id="KW-0698">rRNA processing</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="14">
        <location>
            <begin position="293"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="15">
        <location>
            <begin position="505"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="119"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="130"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="161"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="182"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="202"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="224"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00037449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="EFP75551.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="711" mass="78213" checksum="0B2C961465BA2B60" modified="2012-04-18" version="2">MSSVNSGKLKAKKQEDGSQSKEAGQTITTPSGTAQTKPDKKKKKRKKDETELEPDETVAKDSEAPSQADQSGKKKKKRKKQAQDAPPNPEQTLVESKEPVGKAAGLEESIATTDVNMAEAKDDDNPTDKKKQKKKDKKKKNKQPDDLPTTEQLPSVHENLPPSTAPNHSTSSTSNPPKSVSDTKNIEPSPSISTADVESTKKSKKNKKEKKDKKPSDQSVPVAPTTSPNPTSQSTDPAEIEQFISSNKLTYEPPEIVTQLPPVLKFEDVVVHDSLRKAFSAFQKPSPIQSAVWPVLMAGRDVVGIAETGSGKTLAFGVPALQHILDGDKKSKAINVLVIAPTRELAMQTETTLSDLGKLVSPPIKSLCVYGGVDKNIQRKALANSAVRVVAGTPGRLLDLANEGHLKLDQVSWLILDEADRMLDKGFENDIRAIINLCRPSRTEDSKSRLTTMFSATWPTSVRRLAADFMHEPVRILVGADELTASCSVEQTVEVLEETRGKEWKLLNTLKELGKDSQKNKSKDKIIVFALYKKEAQRLHEFLLRKGFQACCIEGNMSQDKRSKSLEDFMTGKSTILVATDVAARGLDIPKVEFVINVTFPLTIEDYIHRIGRTGRAGRTGKSITYFTDEDKSHAGELMRVLKDAGQVVPEALKQWGGQIKKKTHAAYGDHWKEVDTTIKGKHASFFPPSAIENIMLIRFILALELSNLPR</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-02-22" modified="2025-06-18" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>E3JV98</accession>
    <name>THI4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thiamine thiazole synthase</fullName>
            <ecNumber evidence="1">2.4.2.60</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Thiazole biosynthetic enzyme</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ORF">PGTG_01304</name>
    </gene>
    <organism>
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>GENOME REANNOTATION</scope>
        <source>
            <strain>CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[ADP-thiazole synthase]-L-cysteine + glycine + NAD(+) = [ADP-thiazole synthase]-dehydroalanine + ADP-5-ethyl-4-methylthiazole-2-carboxylate + nicotinamide + 3 H2O + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:55708"/>
            <dbReference type="Rhea" id="RHEA-COMP:14264"/>
            <dbReference type="Rhea" id="RHEA-COMP:14265"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:90873"/>
            <dbReference type="ChEBI" id="CHEBI:139151"/>
            <dbReference type="EC" id="2.4.2.60"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
        <text evidence="1">Binds 1 Fe cation per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homooctamer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="PTM">
        <text evidence="1">During the catalytic reaction, a sulfide is transferred from Cys-219 to a reaction intermediate, generating a dehydroalanine residue.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the THI4 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.60" evidence="1"/>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320392.1">
        <property type="nucleotide sequence ID" value="XM_003320344.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JV98"/>
    <dbReference type="SMR" id="E3JV98"/>
    <dbReference type="FunCoup" id="E3JV98">
        <property type="interactions" value="495"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JV98"/>
    <dbReference type="EnsemblFungi" id="EFP75973">
        <property type="protein sequence ID" value="EFP75973"/>
        <property type="gene ID" value="PGTG_01304"/>
    </dbReference>
    <dbReference type="GeneID" id="10533053"/>
    <dbReference type="KEGG" id="pgr:PGTG_01304"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01304"/>
    <dbReference type="eggNOG" id="KOG2960">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053727_0_0_1"/>
    <dbReference type="InParanoid" id="E3JV98"/>
    <dbReference type="OMA" id="MFPRIVV"/>
    <dbReference type="OrthoDB" id="410463at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160205">
        <property type="term" value="F:cysteine-dependent adenosine diphosphate thiazole synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052837">
        <property type="term" value="P:thiazole biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2840">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03158">
        <property type="entry name" value="THI4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027495">
        <property type="entry name" value="Sti35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002922">
        <property type="entry name" value="Thi4_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00292">
        <property type="entry name" value="sulfide-dependent adenosine diphosphate thiazole synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43422">
        <property type="entry name" value="THIAMINE THIAZOLE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43422:SF3">
        <property type="entry name" value="THIAMINE THIAZOLE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01946">
        <property type="entry name" value="Thi4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0408">Iron</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_0000415879" description="Thiamine thiazole synthase">
        <location>
            <begin position="1"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="110"/>
            <end position="111"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="298"/>
            <end position="300"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="2,3-didehydroalanine (Cys)" evidence="1">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03158"/>
        </source>
    </evidence>
    <sequence length="336" mass="35723" checksum="BF7D75C19CE5E389" modified="2011-01-11" version="1">MSPPVATESMYKPTTIGTEAHDQALAAMKSNQAAPAKPVFKPEPAVNLEPIKFAPIKEHQVQRAMVRRYFQDMEERAISDVIIVGAGSAGLSCAYALGKARPDLKITILESNVAPGGGCWLGGQLMSAMVCRKPADKFLDEVGVPYEDEGNFVVVKHAALFTSTVLSKVLAMPNVKMFNATACEDLIIKPCPINPGVQRVAGCVTNWTLVSLNHDHQSCMDPSTITAPLVCSFAGHDGPFGAFCVKRIASAGLSEGLGDMRPLDMERAEDHIANKTREIVPGLIVGGMELSEFDGSARMGPTFGAMLLSGKRAAEVALQSLGRVKVEEGEVVGSAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3JVF5</accession>
    <name>E3JVF5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_01361</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP76030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320449.1">
        <property type="nucleotide sequence ID" value="XM_003320401.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JVF5"/>
    <dbReference type="GeneID" id="10533272"/>
    <dbReference type="KEGG" id="pgr:PGTG_01361"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01361"/>
    <dbReference type="HOGENOM" id="CLU_1678783_0_0_1"/>
    <dbReference type="InParanoid" id="E3JVF5"/>
    <dbReference type="OMA" id="ICLGSTW"/>
    <dbReference type="OrthoDB" id="2505081at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171572" evidence="2">
        <location>
            <begin position="23"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="78"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="91"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP76030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="157" mass="16197" checksum="8430EDC63C7D2065" modified="2011-01-11" version="1">MRFTDTVPLLLVLLLTIASYIAQRSSGPIGVGDDPDMDMEGMLIDDGPNNATNSPASAYPALTFTSALELQPPHPISTVTTSPVTIPPATPSSTSIPRSNGTNGTSPSLTSSGTSQVVATQTGLPPVPRTSGAGKLDRSFYELIGLLICLGSLWGID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3JVN1</accession>
    <name>E3JVN1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02547</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
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                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
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                <person name="Yandava C."/>
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                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    </feature>
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        <location>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
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        </source>
    </evidence>
    <sequence length="274" mass="27954" checksum="8E438449BA47229A" modified="2012-04-18" version="2">MTFITPCKVQLWNMTNVTIFLIITCGILIPSHKATFPKDSNTTSRSHEVAKRLESPHQPAGNESTSLSPVKQSGYNPPSNSTPATNQSLPGSSDLNGGLLTDPSHPDFGPVPVGVPIAKPSDLVTSALLTAAPSAATAKSNSSSASDGSKNKQNQGYNNTLGQQPVLSGSMAISLPTPSTTSMPSLATQPPVAANGAVSLSSIAPNTVLEVYTAAAGGSSPTPTTSTAYVYATNSPISSENASTTPNSSSHNLLPSSIYLLVGLASTLILVIPN</sequence>
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            <taxon>Pucciniomycetes</taxon>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02967"/>
    <dbReference type="HOGENOM" id="CLU_488463_0_0_1"/>
    <dbReference type="InParanoid" id="E3JWV1"/>
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            <end position="20"/>
        </location>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP76526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="558" mass="63054" checksum="018692FE9E0E411C" modified="2011-01-11" version="1">MNIGITLYVLCSSILFPCEAIDEILDQPHDDLFAWNLQPSNLDDIFFGSPSQTQHPPHYNHDTASSFLSSPQDTPPHASTTSSILQLDPSQAHWGPAFNSPSSYPPIRSQRQALGYHQIEDDLSMSHQEPGALQSNTDHSDVEAYFPKLLGAKRPGPSEYLLSSPAVTRLRVGTQEDTQVNHAISNPPAPSELRRGDDSMNEIAQTMQTQVISQPVGSDSASRGKAIATASTETANPTPSRSSEYHFPNEWTSHPPRNIASVSSSEPNESSEENLENELPNLRFTRDVLVDENATGNYGTQAYKIEELFDELGQENLVLPENEVVKHIACFSGRTKVSTQQALVRKGAPGSQEAENEMGPTLLLKEHYTTGRKKSGKTPTRYSLSKGTKSSFWTHKELWYNYWIMKTGVDFRKHNKDLQCTELLEIFPMYLFFVEMITTIIPRAENRQLSQARELSLACHSFFELLGGRFAGSKKEKYAALQQQILNYSTSPTYFKLWAFLSYWIDINRASLMSAVSPEKGYIPNYFKTFFNTIFYISIEHFHKIYKKLNYFPVHREN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="4">Secreted protein</fullName>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <location>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="137" mass="13761" checksum="1D8E12538DF155B3" modified="2011-01-11" version="1">MKKGQAGSQATSLLRLCELLALLGAPQQAVGFAEGPLQAVSFSRGPQRAVDFAGGPQRAVSFAGGLQRVVIFSGGPQQASGFAGGPHKLLALLEVLSELLASLGVLSELLASLGVLSGLMKGSWQTGLLSSCQGGSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXT6</accession>
    <name>E3JXT6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_02322</name>
        <name evidence="2" type="ORF">PGTG_06799</name>
        <name evidence="3" type="ORF">PGTG_20325</name>
    </gene>
    <organism evidence="1 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <reference evidence="1" key="3">
        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="DS989968">
        <property type="protein sequence ID" value="EFP94369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321280.1">
        <property type="nucleotide sequence ID" value="XM_003321232.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325597.1">
        <property type="nucleotide sequence ID" value="XM_003325549.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338788.1">
        <property type="nucleotide sequence ID" value="XM_003338740.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXT6"/>
    <dbReference type="GeneID" id="10527577"/>
    <dbReference type="GeneID" id="10529046"/>
    <dbReference type="GeneID" id="10536591"/>
    <dbReference type="KEGG" id="pgr:PGTG_02322"/>
    <dbReference type="KEGG" id="pgr:PGTG_06799"/>
    <dbReference type="KEGG" id="pgr:PGTG_20325"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02322"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06799"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20325"/>
    <dbReference type="HOGENOM" id="CLU_131095_0_0_1"/>
    <dbReference type="InParanoid" id="E3JXT6"/>
    <dbReference type="OrthoDB" id="2510615at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP76861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP94369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="175" mass="19637" checksum="0DB7FE12DA7AD1CE" modified="2011-01-11" version="1">MIEIESCIYVPEEPPFVDRQHYRIQDSTPWACTDATMVPILGHLFDVYTSAPRGDSDNAASWLTFQGRCIARYSEPNIAILCNSSSYHNEIPHRHRRIPYPIVRGYLKVLDQGVNCLALDPDVSDSVLFRFSSKSSAIQNSLGELNPGQIVYVSAYLTKHLTGIVDLEVSTVYIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3JY48</accession>
    <name>E3JY48_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02434</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321392.1">
        <property type="nucleotide sequence ID" value="XM_003321344.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JY48"/>
    <dbReference type="STRING" id="418459.E3JY48"/>
    <dbReference type="GeneID" id="10529240"/>
    <dbReference type="KEGG" id="pgr:PGTG_02434"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02434"/>
    <dbReference type="HOGENOM" id="CLU_031991_2_0_1"/>
    <dbReference type="InParanoid" id="E3JY48"/>
    <dbReference type="OMA" id="KRIAMWA"/>
    <dbReference type="OrthoDB" id="2501476at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="33"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="33"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="282"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="462" mass="51285" checksum="4EB73010FAC5B611" modified="2011-01-11" version="1">MNEDGTFGRDCPSIPGPHIPVLFDGTAHLTETASTESRPILTQSSTSDQPVEIQTPGVSVSGSENEKIVIIWKVIDKDLDAFKAEVIRGIKSEEDKFLGFFVERQENARNIFWNVVIPFGGAFAGNHKRRLDSVETFKDFLLVAEGTSETRKIVCRLVQKDPKDIAKKQSVYKQLKKNHTDSSADVNDPVFVPSPSAMNTAALSQLLQEIFLVHSPSEEHSGSAETSVYINPENSDEYFLITMRKAYAWAKAIQANSKEVTVLVPPKSPLFQFKNKTDKSEQANPPNYPNNSQPQQANPPNYPNNSQPQPFYPNQNQAMNPYGMMQMPYQLPAMPYGNYSAAFGNPNQADPSSFYPQASTSAVQIPPSESNVIPPSPPPFEDTTDLSDYLKFARVNADSEEINNGLSKLGITHWSMFEVVSADELVTSGVPLIPARCLIRSVKTYPTHLKNLKKARFLPDIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3K1M1</accession>
    <name>E3K1M1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">GH18 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04152</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP78196.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322615.2">
        <property type="nucleotide sequence ID" value="XM_003322567.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K1M1"/>
    <dbReference type="STRING" id="418459.E3K1M1"/>
    <dbReference type="GeneID" id="10541826"/>
    <dbReference type="KEGG" id="pgr:PGTG_04152"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04152"/>
    <dbReference type="eggNOG" id="KOG2806">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002833_6_3_1"/>
    <dbReference type="InParanoid" id="E3K1M1"/>
    <dbReference type="OrthoDB" id="73875at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008061">
        <property type="term" value="F:chitin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004568">
        <property type="term" value="F:chitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006032">
        <property type="term" value="P:chitin catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.50.10:FF:000011">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011583">
        <property type="entry name" value="Chitinase_II/V-like_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029070">
        <property type="entry name" value="Chitinase_insertion_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001223">
        <property type="entry name" value="Glyco_hydro18_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050314">
        <property type="entry name" value="Glycosyl_Hydrlase_18"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177">
        <property type="entry name" value="CHITINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11177:SF317">
        <property type="entry name" value="CHITINASE 12-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00704">
        <property type="entry name" value="Glyco_hydro_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00636">
        <property type="entry name" value="Glyco_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51910">
        <property type="entry name" value="GH18_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GH18" evidence="1">
        <location>
            <begin position="46"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78196.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="429" mass="47472" checksum="EF30827217F8DA32" modified="2012-04-18" version="2">MQSSWSFSSSKSHSNSAELNVTAGNLTNDTMKSKGSNINTKKTGKRNLAGYYPVYNFKGQPLSELRYDLYDEIIFFATSTTQNFTLGIDYMSESEWGELAADFVKTCKNYSVRPMYSVGGWCGSKYFSSLAATAENRTAFADSTISFAKKYGFEGIDFDWLYPSIQGLGCNTISENDVVNQQLLFKEVKQKWPEGKLSAAQSPSGIRNSNYQELKSSDLTLMAEVVDELRILAHDVYGSSTKTTGPSAPISSRCADSENKVSVEDAIEVYLKQGFKPEQLSLAIPGFGKSWTLAQPELTPKVVGEYTSYYYQNYTGIPPGGKWDDEPGVDDCGKKTGWGGIWLVKELVQDGFLSKDEMHGMKGFTRYYDACSGQPFLARNTTMISYDDTQSTLAKVKYAKSKGLGGVVFFDTMGPRDKTVKAARKALFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2Q8</accession>
    <name>E3K2Q8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_04583</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78627.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323046.2">
        <property type="nucleotide sequence ID" value="XM_003322998.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2Q8"/>
    <dbReference type="GeneID" id="10536480"/>
    <dbReference type="KEGG" id="pgr:PGTG_04583"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04583"/>
    <dbReference type="HOGENOM" id="CLU_2005031_0_0_1"/>
    <dbReference type="InParanoid" id="E3K2Q8"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP78627.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="124" mass="14144" checksum="4F21FD02E13BCDC1" modified="2012-04-18" version="2">MEGHVLIIRNFQLGRSLSIGGWSPAQIRLCDYKCLRPIGTPFKGRWIRCLPPHLPSIHPDFPGKPIICLFIWKATGHRLFYHRDSITSFFPPNSSARTSTSCNGSHLTRYVDIFQLFDCAICLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3F3</accession>
    <name>E3K3F3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_04966</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP79010.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323429.2">
        <property type="nucleotide sequence ID" value="XM_003323381.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3F3"/>
    <dbReference type="STRING" id="418459.E3K3F3"/>
    <dbReference type="GeneID" id="10530831"/>
    <dbReference type="KEGG" id="pgr:PGTG_04966"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04966"/>
    <dbReference type="eggNOG" id="KOG0442">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1741458_0_0_1"/>
    <dbReference type="InParanoid" id="E3K3F3"/>
    <dbReference type="OrthoDB" id="361020at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10150">
        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10150:SF0">
        <property type="entry name" value="DNA REPAIR ENDONUCLEASE XPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="3" id="KW-0234">DNA repair</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP79010.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="150" mass="16535" checksum="3F96FBAA2E82716D" modified="2012-04-18" version="2">MFKKLRLIWSSSPSATAEIFQDLKLTHPEPDAETASLVGMDGISKGEKGKLDLMAINKTQQNQNFGICSSAHKVLKALPGMDELKARKLIYGVVNTPATNNNNNTLASEGFLPTRAPARRKESLPTSWCSGQGDQFKYHPVDSPFDNWTQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3K534</accession>
    <name>E3K534_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_05670</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323768.1">
        <property type="nucleotide sequence ID" value="XM_003323720.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K534"/>
    <dbReference type="GeneID" id="10531173"/>
    <dbReference type="KEGG" id="pgr:PGTG_05670"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05670"/>
    <dbReference type="HOGENOM" id="CLU_176469_0_0_1"/>
    <dbReference type="InParanoid" id="E3K534"/>
    <dbReference type="OrthoDB" id="10351275at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173007" evidence="2">
        <location>
            <begin position="17"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="25"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP79349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="112" mass="12672" checksum="D2E96105855A349F" modified="2011-01-11" version="1">MRLLLIVAALIPFAYLLEPERQLQPDGKGFRKRAYRDPIAIVKAPAKRKKRNTPNKPNKPNKPNKPSEPSEPSETNTSSIDLGPGFLQRPHKDDTIYALYSGADGYYTEMFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3K5B3</accession>
    <name>E3K5B3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Leucine-zipper-like transcriptional regulator 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_06030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79709.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324128.2">
        <property type="nucleotide sequence ID" value="XM_003324080.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K5B3"/>
    <dbReference type="STRING" id="418459.E3K5B3"/>
    <dbReference type="GeneID" id="10533070"/>
    <dbReference type="KEGG" id="pgr:PGTG_06030"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06030"/>
    <dbReference type="HOGENOM" id="CLU_2027880_0_0_1"/>
    <dbReference type="InParanoid" id="E3K5B3"/>
    <dbReference type="OrthoDB" id="45365at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093">
        <property type="entry name" value="ACYL-COA-BINDING DOMAIN-CONTAINING PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46093:SF18">
        <property type="entry name" value="FIBRONECTIN TYPE-III DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP79709.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="122" mass="13864" checksum="34A6A5D188D61548" modified="2012-04-18" version="2">MATFFFGSGFLYVYAGHDVNRSVQGDMWRIELDSLPSVPSWELISSSKDQICPPARTSHSAVVSSNSWIIFGGTDGVDVFNDLWEYRFREKKWRQVSANGSVPPPRKDHLVTQVKDHIKHPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3K636</accession>
    <name>E3K636_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_05928</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324026.1">
        <property type="nucleotide sequence ID" value="XM_003323978.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K636"/>
    <dbReference type="GeneID" id="10531442"/>
    <dbReference type="KEGG" id="pgr:PGTG_05928"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05928"/>
    <dbReference type="HOGENOM" id="CLU_124024_0_0_1"/>
    <dbReference type="InParanoid" id="E3K636"/>
    <dbReference type="OrthoDB" id="2505965at2759"/>
    <dbReference type="UniPathway" id="UPA00143"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044695">
        <property type="term" value="C:Dsc E3 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:000612">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051834">
        <property type="entry name" value="RING_finger_E3_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931:SF3">
        <property type="entry name" value="RING ZINC FINGER-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45931">
        <property type="entry name" value="SI:CH211-59O9.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="5">
        <location>
            <begin position="140"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP79607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="207" mass="23419" checksum="E5C9D744291C90B8" modified="2011-01-11" version="1">MAQATAERAEEALSYLTPEERPIFQRCFRLLAEAHSELDTHPLRDGFDADEFLYYFGDVFGLEEAWSRLEESVHRRLQDVVNQWTALIEALDDSRTASFASRLRVERHRLRVQLLLDTLTVSNATAATLLSSPDGVASQCSICKDQLAQPEHTIVQLPCHPTHLFHRDCVQPWVEDNLNCPICRVEIELPPASDSDSDSDSDSEPDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3K6S6</accession>
    <name>E3K6S6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_05151</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EFP79926.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003324297.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K6S6"/>
    <dbReference type="GeneID" id="10533617"/>
    <dbReference type="KEGG" id="pgr:PGTG_05151"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05151"/>
    <dbReference type="HOGENOM" id="CLU_033090_3_1_1"/>
    <dbReference type="InParanoid" id="E3K6S6"/>
    <dbReference type="OrthoDB" id="2495477at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP79926.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="386" mass="44272" checksum="8B2D91DD969D076D" modified="2012-04-18" version="2">MNEIEEMDDRDALARHQLQHRADFVAKCFGRLAECDFDPGSTEAPDLSIGRLRPKKYLLTELHSSLLPALRQHVIGLWQALSYPQRMPRNAAHILHYVIGSQPELEMIVDRIIWVINDLIPGRIQKSNQTNDQHFKEFKQYRLHGLDTSARSEMQNELASFFTQCRGASEQLMLPAGYQIYVQPSSTNDLLRSIDSVIRWSKGPELHFIYDHWKLAMKSMEKTLDDLLLAMAPEHALYSEPFTELAQTFIPVIKLSKLFFRKMTTDGMSQKNLPFYTEMSSDQLSSLKRSADVITDSVSHLFFCLPRSDERHAISSATIFTGEIKKILTRFQSCLLLVDLYIIPRCTDINVCSSPTSLKTWIVTWNTLLYQASHNSIQACESIVIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3K7S1</accession>
    <name>E3K7S1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Prefoldin subunit 4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_06325</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324788.1">
        <property type="nucleotide sequence ID" value="XM_003324740.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K7S1"/>
    <dbReference type="FunCoup" id="E3K7S1">
        <property type="interactions" value="372"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K7S1"/>
    <dbReference type="GeneID" id="10539377"/>
    <dbReference type="KEGG" id="pgr:PGTG_06325"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06325"/>
    <dbReference type="HOGENOM" id="CLU_1462016_0_0_1"/>
    <dbReference type="InParanoid" id="E3K7S1"/>
    <dbReference type="OMA" id="HQLIATH"/>
    <dbReference type="OrthoDB" id="10250441at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016661">
        <property type="entry name" value="PFDN4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21100">
        <property type="entry name" value="PREFOLDIN SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21100:SF9">
        <property type="entry name" value="PREFOLDIN SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0143">Chaperone</keyword>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="134"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP80369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="185" mass="21210" checksum="9ABBD92AEEC98C8B" modified="2011-01-11" version="1">MEMLEPIRATSGKDSSSKINRNDEHEPAGMEEIEVNFKDQKMINQFSNLNLKKIKLKKLIKSKSVELDDLIELENELLLSSFDDIDQSNNNENLDDEAGLKTTEDNDLILYKLDSSYIHIKRSTFQETKLPESLDRSREMISKLNSDLTDLNTQMSELKKTLYSKFGNTINLEGGDEDEDEEVAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3K7Z1</accession>
    <name>E3K7Z1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_06360</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
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                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <source>
            <dbReference type="Pfam" id="PF13638"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
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    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <dbReference type="Proteomes" id="UP000008783">
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    <proteinExistence type="predicted"/>
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            <begin position="118"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP80244.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3K8V8</accession>
    <name>E3K8V8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    <gene>
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    <organism evidence="2 3">
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <dbReference type="InParanoid" id="E3K8V8"/>
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    <proteinExistence type="predicted"/>
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            <end position="161"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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    <feature type="region of interest" description="Disordered" evidence="1">
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    <feature type="region of interest" description="Disordered" evidence="1">
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="114"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="209"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="356"/>
            <end position="413"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="415"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="433"/>
            <end position="443"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="464"/>
            <end position="475"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP80547.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="503" mass="50605" checksum="57E56CD24627290B" modified="2012-04-18" version="2">MFLAKATWKICLASVLITLFYRPAIFLSEARPNMLTRRQVTQPPNPGSSLAPGSNLPTASGTPAPGILGSANPLNLTGAGGGNRASTLGTTNINTPDTPSPFPPAPGTTGGYGITTYSGTATTGSGTSLGGTGTGTETSSIPATVGNGNTNIPTTDDSGGYGIPTTSGGTTVGTGTSYGIGTSPTPSTVGGGNGNLATTNPTYGVPTTFGSSNSETETLPSYGGTNYNTSPTTYNPPITYNSPTPYNPPTTDNPLTTYNPPTTYNPPTPYNPPATPYNPPTTDNPPTYNPLTTAYIPPTPYTPLTTYTPPIPSTVGGGTGMQTSPQPGTGGSGTTPFQTGLNNNSPAPGTVGGENPSTSGTVPTTSGTVPTTSGTVPTTYGTVPTTSSTPATNSSTPAPPTKSGSPPTKSASPEIKPKDKGGTETKPKDKGKTKPKAAPPKKLKVAEIQSELVKCGYSYGAGKNDTNQPSGTVSCRSPEGLGYYCPASKCTQANPKDQKPARK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>E3K9Q7</accession>
    <name>E3K9Q7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_07183</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP80931.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325350.2">
        <property type="nucleotide sequence ID" value="XM_003325302.2"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K9Q7"/>
    <dbReference type="GeneID" id="10536089"/>
    <dbReference type="KEGG" id="pgr:PGTG_07183"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07183"/>
    <dbReference type="eggNOG" id="KOG0269">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_262408_0_0_1"/>
    <dbReference type="InParanoid" id="E3K9Q7"/>
    <dbReference type="OrthoDB" id="60955at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061700">
        <property type="term" value="C:GATOR2 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005774">
        <property type="term" value="C:vacuolar membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016239">
        <property type="term" value="P:positive regulation of macroautophagy"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037590">
        <property type="entry name" value="WDR24"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46200">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46200:SF1">
        <property type="entry name" value="GATOR COMPLEX PROTEIN WDR24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="1 6" id="KW-0853">WD repeat</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="318"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="409"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="56"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="226"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="732"/>
            <end position="907"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1196"/>
            <end position="1255"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="70"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="118"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="133"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="756"/>
            <end position="774"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="778"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="829"/>
            <end position="858"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="1230"/>
            <end position="1241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="1245"/>
            <end position="1255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP80931.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1289" mass="142932" checksum="64206A6C0897A12F" modified="2012-04-18" version="2">MKPTTAPTRSTSHHQVGQNRSTHHSRSFTSSNLENSVGAGANPQLLFPSAHRATTPLNQTIDSSDHHSQRTSSHLSTSPSLSRPRLIRHLLFSTPDSSSSSTVNHHHQQQQQQQQQLFKHHKSHPSHRPSTSQPSTSIHSLSRSSYNTHHQLSNPPPLRVGLPVICVARAHQPNHPHQHHRSDSYHYSHAKQLVAIASRTEIRILELINHHHSLPFEAPRRQQHSSFSPSLATHTRVETRPSFPSRSLDSSTTFDDRLAIKTNSELGPSYGVSDLGWGYASTTSQLATGCTNGSIVIWDINHPITQDPIQRHRSLTLDHAHNRAINKITFAGPTGHWIASGGQDSLVKIWDVREGPRPTMTLTTHTDPVRQLRFFPDRTVNPFDLFVLTDSGTLSHFDLRQDKSCLTRRVAHASTGVGLDWLSDGYNSMLASAGADGIVKIWNMADSILPSTAMRTLVVGRSICGMTWRPGYSTQLAITPSSVMADLEQQGYSDYERGPSSAMSSNYSDDLMLNKGLSATGASSVSDVVSESSHMGGGRHSEILLWDIRRPYVPEMIIRGRDGPASGMVWLSADLLLTTHKRTSAIVQHDISQSQAKYADELPSQAMAVSKQGSLCFSLGSKKTDSRFNIEEELRSAMVHSIDELQPPRKFEFLALNYKFEYHSFERVCEHNANLCRRVGQPELWQLWMAVKLWFTPQVSAALIVAQEMALGSNGNDERAIAMAMRRVLKEESSDEGEMDYNQSGGDLESSCGRRSPSQDQASRQNSTSGSRTCSGDMRSKREESLDRSETQSPLPKSDQDPGHRPKLSLSGLALPSVLDPGELLRGGQSLNRSSSATTPKAQMRSSFLSNVTSNPSLESSAEPARRRPAPLRSLAIDCDSPISSPDTEGPRRSIAHRGIERTPDGRLNPAWRRSMSKDRIAAKMHARGASFNELGSLIQAIGVGKLLSEKKGEDWKNQEEKMAVEIQMRESILELVQKEVEINGNVQLGVYVIGVLKVYHPAHESHDEESNHNSNTINNNRNDSRVIGIEKELLEDRFKSWGRVYLKSLTRHPSLLVVASEIKKKFSCLISKELEDQSLRILKLNPTCGNCGKVLMNSVVPSHPLPTINSNSNQQSLTMMMMLNNHKKKMLLNNHQNQNSNNNSNKTLKCDKCQVNVLKCCFCHESIKFEPLIWCLKCGHGIHSHCLKKIKTSTTSKSAEENASDPLKNPPTNPLEPEARNNNHLGSRSSHERSALERNPKSFSSSTTTTTTTTVAEVHPQLSLGYPSSRPFLQCPSGCGCIGCFYSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3KA12</accession>
    <name>E3KA12_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_07246</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP80994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325413.1">
        <property type="nucleotide sequence ID" value="XM_003325365.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KA12"/>
    <dbReference type="STRING" id="418459.E3KA12"/>
    <dbReference type="GeneID" id="10536233"/>
    <dbReference type="KEGG" id="pgr:PGTG_07246"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07246"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_970236_0_0_1"/>
    <dbReference type="InParanoid" id="E3KA12"/>
    <dbReference type="OMA" id="SCETCEX"/>
    <dbReference type="OrthoDB" id="8922241at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000621">
        <property type="entry name" value="FLT3-interacting zinc finger 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050331">
        <property type="entry name" value="Zinc_finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515">
        <property type="entry name" value="PR DOMAIN ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16515:SF58">
        <property type="entry name" value="ZINC FINGER PROTEIN 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="249"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="9">
        <location>
            <begin position="277"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="91"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="16"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="102"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="113"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP80994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="305" mass="34466" checksum="FB16C76A1FD5F5EA" modified="2011-01-11" version="1">MKLSMITNQTNSTANEESRKGQDDYRRYGGSYLDEVNYEDGMERDPSRLKRVRLNEEERKGDEEEGQDGQAGMDLRSQSWSKLQVLLLNQDKPIGRSTIINEMDEPELELEGESPSNSFHSSSSSTLTQSQHSSPLQPTTPDQSQPFLPSWATEPSGWSKQRVTDEMELLSVIEKQAETYSSGPESEALLTPGAQSQPTAWINHHHPPRQHHSLRVLQPPELIVPSFPSQPVANLPSPAIPAIPQEKPYKCSLCPKSFTRNYDLTRHKSSHIDQRQHRCSKCGRSFNRRDALIRHTLVKSCGNNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KA68</accession>
    <name>E3KA68_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06851</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325649.1">
        <property type="nucleotide sequence ID" value="XM_003325601.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KA68"/>
    <dbReference type="GeneID" id="10536715"/>
    <dbReference type="KEGG" id="pgr:PGTG_06851"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06851"/>
    <dbReference type="HOGENOM" id="CLU_044116_1_0_1"/>
    <dbReference type="InParanoid" id="E3KA68"/>
    <dbReference type="OrthoDB" id="10550145at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="167"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="371" mass="40599" checksum="5E13B5CE50308A7C" modified="2011-01-11" version="1">MPTVSKILCPETQYDEDTLIDNPPRKAPICLDFSLYVKSVNIPDFPHARWANAGNGWVIISPTRELAVMPVDLQSMTWSHFQGLAIQHLGQHQPPMARLLGAAHNNGALLWQAFILGNVQFSQKNKTIIHGPKEFRVFADQAYHEFPNRVSFKILMLEPPGNVVAKGAKESTDNDKAPAGKNDGVTKAPAARTRIRPTKSKQITDVLPEKRGLIAEDGSAAVGSTSAMSNHVVEGETVPSDRPAKHAKKVKTPVGHDTDEIEVIRVPSAVPPATTPLPNSHSGVNRVPLGPESPQLEAVDMETYLMVSHISPTDQATRRRLRTHGIIHWTFFRATSEAELLDLGFTLGIACLLCEGVPRLEAYVAERSVPL</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-10-19" modified="2026-01-28" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAB5</accession>
    <name>ITPA_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Inosine triphosphate pyrophosphatase</fullName>
            <shortName evidence="1">ITPase</shortName>
            <shortName evidence="1">Inosine triphosphatase</shortName>
            <ecNumber evidence="1">3.6.1.66</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Non-canonical purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Non-standard purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Nucleoside-triphosphate diphosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">Nucleoside-triphosphate pyrophosphatase</fullName>
            <shortName evidence="1">NTPase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="1">XTP/dITP diphosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ORF">PGTG_06893</name>
    </gene>
    <organism>
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>GENOME REANNOTATION</scope>
        <source>
            <strain>CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) or xanthosine 5'-triphosphate (XTP) to their respective monophosphate derivatives. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ITP + H2O = IMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:29400"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>dITP + H2O = dIMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28342"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61194"/>
            <dbReference type="ChEBI" id="CHEBI:61382"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:28343"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>XTP + H2O = XMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28610"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="1">
            <dbReference type="Rhea" id="RHEA:28611"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="1">Binds 1 divalent metal cation per subunit; can use either Mg(2+) or Mn(2+).</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HAM1 NTPase family.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.66" evidence="1"/>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325691.1">
        <property type="nucleotide sequence ID" value="XM_003325643.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KAB5"/>
    <dbReference type="SMR" id="E3KAB5"/>
    <dbReference type="FunCoup" id="E3KAB5">
        <property type="interactions" value="565"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KAB5"/>
    <dbReference type="EnsemblFungi" id="EFP81272">
        <property type="protein sequence ID" value="EFP81272"/>
        <property type="gene ID" value="PGTG_06893"/>
    </dbReference>
    <dbReference type="GeneID" id="10536759"/>
    <dbReference type="KEGG" id="pgr:PGTG_06893"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06893"/>
    <dbReference type="HOGENOM" id="CLU_082080_1_1_1"/>
    <dbReference type="InParanoid" id="E3KAB5"/>
    <dbReference type="OMA" id="YDPIFQP"/>
    <dbReference type="OrthoDB" id="6288734at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035870">
        <property type="term" value="F:dITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036220">
        <property type="term" value="F:ITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047429">
        <property type="term" value="F:nucleoside triphosphate diphosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036222">
        <property type="term" value="F:XTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009204">
        <property type="term" value="P:deoxyribonucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009143">
        <property type="term" value="P:nucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00515">
        <property type="entry name" value="HAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.950.10:FF:000003">
        <property type="entry name" value="Inosine triphosphate pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_03148">
        <property type="entry name" value="HAM1_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027502">
        <property type="entry name" value="ITPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002637">
        <property type="entry name" value="RdgB/HAM1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00042">
        <property type="entry name" value="RdgB/HAM1 family non-canonical purine NTP pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067:SF9">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE/HAM1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01725">
        <property type="entry name" value="Ham1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0378">Hydrolase</keyword>
    <keyword id="KW-0460">Magnesium</keyword>
    <keyword id="KW-0464">Manganese</keyword>
    <keyword id="KW-0479">Metal-binding</keyword>
    <keyword id="KW-0546">Nucleotide metabolism</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0539">Nucleus</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000413148" description="Inosine triphosphate pyrophosphatase">
        <location>
            <begin position="1"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="74"/>
            <end position="75"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="149"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="177"/>
            <end position="178"/>
        </location>
        <ligand>
            <name>ITP</name>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03148"/>
        </source>
    </evidence>
    <sequence length="192" mass="20845" checksum="55506B5295813B3B" modified="2011-01-11" version="1">MGALKLVFVTGNANKLREVKKILSTDVSSEDSLKIEVDSKALDLPEVQGSTQDVAREKSRAAAKLIGGPCITEDTALCFKAMGGLPGPYIKWFLEKLGLDGLNKMLQGFSSTEATALCTFAYCEPGKEPILFEGATEGNIVPARGPTNFGWDPIFEVSGTGMTYAEMPAEQKNSLSHRSKALDKLRQHFSRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>E3KB79</accession>
    <name>E3KB79_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">T-complex protein 1 subunit eta</fullName>
            <shortName evidence="9">TCP-1-eta</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">CCT-eta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_07841</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. Known to play a role, in vitro, in the folding of actin and tubulin.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterooligomeric complex that forms two stacked rings.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81592.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326011.2">
        <property type="nucleotide sequence ID" value="XM_003325963.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KB79"/>
    <dbReference type="FunCoup" id="E3KB79">
        <property type="interactions" value="846"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KB79"/>
    <dbReference type="GeneID" id="10532973"/>
    <dbReference type="KEGG" id="pgr:PGTG_07841"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07841"/>
    <dbReference type="eggNOG" id="KOG0361">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008891_7_1_1"/>
    <dbReference type="InParanoid" id="E3KB79"/>
    <dbReference type="OrthoDB" id="10248520at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005832">
        <property type="term" value="C:chaperonin-containing T-complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03340">
        <property type="entry name" value="TCP1_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.260.10:FF:000022">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000006">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012720">
        <property type="entry name" value="Chap_CCT_eta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02345">
        <property type="entry name" value="chap_CCT_eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="534"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="534"/>
            <end position="543"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU365042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP81592.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="562" mass="61466" checksum="1A873B81D5EA4CAA" modified="2012-04-18" version="2">MAGRPQLPNIQPQIILLREGTDTSQGTPQLLSNINACLAVAQTVASTLGPRGMDKLIVATNGEATISNDGATIMKLLQVVHPAAKTLVDIARAQDAEVGDGTTSVVLLCAELLKQCKPYIEEGVSPHVIIKGYRKACEMAVAKVKELAITVDKSDQTKFRDLLIKCASTSMSSKLIHHHKPFFSNMVVDAVMTLDQNDLDESLIGIKKIPGGGMEDSLLIKGVAFKKAFSYAGFEQQPKSFQSPKLLCLNVELELKAERDNAEVRIERVEDYQRVVDAEWQIIFRKLENIVETGAQVVLSRLPIGDLATQYFADRNIFCAGRIPIDDLKRVVQAVGGSIQSTTTDIHDSHLGSCESFEERQIGAERYNVFQGGKHSKTCTLVLRGGAEQFISEVERSLHDAIMVVKRAIKNNQVVAGGGACEMEVSKYLREESKLIQGKQQLMLASYAKSLECIPRQLCDNAGLDATDILNKLRMLHAQDQKWIGVNLEVDLDLGVADNYEKFVWEPALVKTNALEGATEAACMILSVDATVRNPQSEKQSAGPQMPRGAARGALRGRGRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBD6</accession>
    <name>E3KBD6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_07898</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81649.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326068.2">
        <property type="nucleotide sequence ID" value="XM_003326020.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KBD6"/>
    <dbReference type="GeneID" id="10533193"/>
    <dbReference type="KEGG" id="pgr:PGTG_07898"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07898"/>
    <dbReference type="HOGENOM" id="CLU_3088325_0_0_1"/>
    <dbReference type="InParanoid" id="E3KBD6"/>
    <dbReference type="OrthoDB" id="10270891at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP81649.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="52" mass="5783" checksum="346C90C429ECB128" modified="2012-04-18" version="2">MTATNVLGYCEPNRHQNMLRADESFKAGDKKALDRSTSHEDLQAYTVDVPSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBR2</accession>
    <name>E3KBR2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Citrate transporter-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_07970</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81721.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326140.2">
        <property type="nucleotide sequence ID" value="XM_003326092.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KBR2"/>
    <dbReference type="STRING" id="418459.E3KBR2"/>
    <dbReference type="GeneID" id="10533429"/>
    <dbReference type="KEGG" id="pgr:PGTG_07970"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07970"/>
    <dbReference type="eggNOG" id="ENOG502QVIJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017834_0_0_1"/>
    <dbReference type="InParanoid" id="E3KBR2"/>
    <dbReference type="OrthoDB" id="442352at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004680">
        <property type="entry name" value="Cit_transptr-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43302">
        <property type="entry name" value="TRANSPORTER ARSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43302:SF5">
        <property type="entry name" value="TRANSPORTER ARSB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03600">
        <property type="entry name" value="CitMHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="92"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="124"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="163"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="203"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="345"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="516"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="555"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="604"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="651"/>
            <end position="672"/>
        </location>
    </feature>
    <feature type="domain" description="Citrate transporter-like" evidence="9">
        <location>
            <begin position="98"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="399"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP81721.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="673" mass="73488" checksum="D82EFC76DBC0EA4A" modified="2012-04-18" version="2">MAEEQVRSLDGFSIFGLIVVLLAMVPVLYPIRIGLGESFTRVLKKLAIRCRLIEAPSDSSDSQAATATTKPPNSPDQANWQLVLNLTTSPPIAVLVLLATTTINGAVIRSGIVGEGDSKPYDVLVLFISLAYIATALDSTGALRSLAFLVSNHTSGSGPKLYFVLYCFFFVFGVIFGNDPIILSGTAFLTYFLKHCGITNPTAWIFMEFVASNVASAVLVSSNPTNVLLAQAFDLNFLTGFTKFTIIPSLVSAIVGYVLLYTLFKSLTISDTHISSSPPPEKDPNFQSSESSKRVKRNFASFSVTRIFQFPKTLYNSIPRANYIPDKLNQPIVNPRSVLVDPQGAFFHGTLMITTLLLLVGITFIKTASVWHVTMPAGILALIRDIMWDIRSQNRKSSLTEEHELESDLGPVPPVQDSSKIDENVDGDHGLLGKDVDPPMSIAEPIRPAPLHLAGNAKRSIDQLTHPNDIATLSSAPTKLPTVQPVGKWNLASFASRLARRFPCTSRTIAHLPLPLLPFAMAMFILISSLSHLGWVTVFARWLASVCTSPARAVYVVGLLGSMVLCPFMGTNIGATILMVNVISDRQFRLNERVVEDPRILRSAIFATAMSSNIGAFSLTIPSSLAGLLWHQILQQKSILIRNRDFLGWNLLPVLVLSLVSLSIVFVEVMFIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="78" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBY6</accession>
    <name>E3KBY6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_08037</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EFP81788.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326207.2">
        <property type="nucleotide sequence ID" value="XM_003326159.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KBY6"/>
    <dbReference type="FunCoup" id="E3KBY6">
        <property type="interactions" value="471"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KBY6"/>
    <dbReference type="GeneID" id="10533658"/>
    <dbReference type="KEGG" id="pgr:PGTG_08037"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08037"/>
    <dbReference type="eggNOG" id="KOG0271">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_16_1"/>
    <dbReference type="InParanoid" id="E3KBY6"/>
    <dbReference type="OrthoDB" id="10267436at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110136">
        <property type="term" value="P:protein-RNA complex remodeling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000464">
        <property type="entry name" value="Ribosome assembly protein 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011047">
        <property type="entry name" value="Quinoprotein_ADH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001632">
        <property type="entry name" value="WD40_G-protein_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848:SF0">
        <property type="entry name" value="NOTCHLESS PROTEIN HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19848">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00319">
        <property type="entry name" value="GPROTEINB"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50998">
        <property type="entry name" value="Quinoprotein alcohol dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 5" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="187"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="229"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="272"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="319"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="443"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="485"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="5">
        <location>
            <begin position="527"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="70"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="90"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP81788.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="558" mass="61804" checksum="4850ECA7C05FBF95" modified="2012-04-18" version="2">MATVIPPPPKKKAKFEKLNANTLNDAILPSFPNIIVQLKDGRTGKPIGSSISLPSNTDKRGLELLVNQLKKDQRAKKPKLSHQERKQLAEEENDDQQDDDDDDEADVNLPWSFSLTLKKPLPPKLPSGPEVPSPEEEEIRIPIYESLSKDLLINHSNLISTEDILVIDCEPEAIFRVREVRRCSSSLAGHSAPILCASFSPTGTYLATGSGDNTCRLWDLSTETPKFSLAGHTGWLLCVEWDGCERLLATGSMDKTVRIWDPLTGKQMGTPLKGHSQWITSLAWEPIHLNSETTRLASSSKDGTVRVWNPRTGMTQFALGGHTASVNAVRWSGQGILFTASSDRTVKCWDAKDGKLIRTLNEHSHWVNTLALNTDYILRTGPFDPKDTAKPKSNEAAKEAALKRYSSFTSLAPELLISGSDDHTLFLWPSLDSATPKKPLARLTGHQKQVNHVAFSPDGKFLASASFDNHIKLWEGKTGKFIATLRGHVAPVYRLSWSCDSRLLVSASKDSTLKLWDLRTHKIKVDLPGHTDEVYCVDFVADKVASGGRDKVVKIWKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCA2</accession>
    <name>E3KCA2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08105</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EFP81856.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326275.2">
        <property type="nucleotide sequence ID" value="XM_003326227.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCA2"/>
    <dbReference type="GeneID" id="10533893"/>
    <dbReference type="KEGG" id="pgr:PGTG_08105"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08105"/>
    <dbReference type="HOGENOM" id="CLU_093243_0_0_1"/>
    <dbReference type="InParanoid" id="E3KCA2"/>
    <dbReference type="OrthoDB" id="10309545at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="165"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81856.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="194" mass="20627" checksum="9E72F230AAB75154" modified="2012-04-18" version="2">MSDDSNCDFKLAPKPAGTISGAAATISRLTPKTQAIRQALASKKNTDASEALQHKLGRTILEGSKNNCLPLGASSKGKAPAALDNAPVETHFHSRITDKPTGSSLANWCCADPPHQHTKPLQTELLIAELQAKRKDNRLCQEEVWLQNPSIPNGLSKLLDPAHPAQSAQLSSQTQLSQLLDPAQSALKPSSVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCU3</accession>
    <name>E3KCU3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_07636</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion inner membrane</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Matrix side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the complex I NDUFA12 subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326658.1">
        <property type="nucleotide sequence ID" value="XM_003326610.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCU3"/>
    <dbReference type="STRING" id="418459.E3KCU3"/>
    <dbReference type="GeneID" id="10529612"/>
    <dbReference type="KEGG" id="pgr:PGTG_07636"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07636"/>
    <dbReference type="eggNOG" id="KOG3382">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_110455_2_1_1"/>
    <dbReference type="InParanoid" id="E3KCU3"/>
    <dbReference type="OMA" id="VIYTAEM"/>
    <dbReference type="OrthoDB" id="274641at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045271">
        <property type="term" value="C:respiratory chain complex I"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007763">
        <property type="entry name" value="NDUFA12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12910:SF2">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12910">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE SUBUNIT B17.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05071">
        <property type="entry name" value="NDUFA12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0249">Electron transport</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="2" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0679">Respiratory chain</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU363103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP82239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="139" mass="16316" checksum="21780E28AC82D501" modified="2011-01-11" version="1">MATPSLARTIKNFLALGPREYIRQLWYICDPKPGAFRGSDEHGNKYYQDPSESMFRNRWVDYKVHDFNASQVSPEWHSWLQHIRKDPPHLDPIVVQSRQSWQMPHVENITGTRGAFKTYSTTLPKVQSWEPRVSERTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3KD85</accession>
    <name>E3KD85_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">rRNA-processing protein and EBNA1-binding protein ebp2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_07506</name>
        <name evidence="9" type="ORF">PGTG_12523</name>
    </gene>
    <organism evidence="8 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <reference evidence="8" key="3">
        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EBP2 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="DS178311">
        <property type="protein sequence ID" value="EFP88076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326528.1">
        <property type="nucleotide sequence ID" value="XM_003326480.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332495.1">
        <property type="nucleotide sequence ID" value="XM_003332447.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KD85"/>
    <dbReference type="FunCoup" id="E3KD85">
        <property type="interactions" value="530"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KD85"/>
    <dbReference type="GeneID" id="10534640"/>
    <dbReference type="GeneID" id="10535217"/>
    <dbReference type="KEGG" id="pgr:PGTG_07506"/>
    <dbReference type="KEGG" id="pgr:PGTG_12523"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07506"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12523"/>
    <dbReference type="eggNOG" id="KOG3080">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036007_0_0_1"/>
    <dbReference type="InParanoid" id="E3KD85"/>
    <dbReference type="OMA" id="RETMFHR"/>
    <dbReference type="OrthoDB" id="443772at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034399">
        <property type="term" value="C:nuclear periphery"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008610">
        <property type="entry name" value="Ebp2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13028">
        <property type="entry name" value="RRNA PROCESSING PROTEIN EBNA1-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13028:SF0">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN EBP2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05890">
        <property type="entry name" value="Ebp2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="149"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="45"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="190"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="207"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP82109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP88076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="269" mass="29628" checksum="FE9FC25891DA6C36" modified="2011-01-11" version="1">MASLESLFSNPTATAPGGFGASVGLDFFETQLVDSQARTSVEDVNDDLKIEVELYKNALEAAQQAVRKFETSGKPFFRPSDYFAEMIKSDAHMETIRLRLVEEAEGLKASEEAKKKRELKKFGKAIQVENKLQREKETKRIKEGVKELRKNRKDVTKLDGQGEQEFEIAIEETLSGNPRKRAGGPSGSGKESEGRKKSRTGRERQFGRPKPKGVTGRRWKENTKSSAGSFEGMGGKGDGRISSGTGRNRGRGRGAGGSSKRGSHRSKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGI9</accession>
    <name>E3KGI9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mediator of RNA polymerase II transcription subunit 11</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Mediator complex subunit 11</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">MED11</name>
        <name evidence="6" type="ORF">PGTG_08600</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the Mediator complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the Mediator complex subunit 11 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327833.1">
        <property type="nucleotide sequence ID" value="XM_003327785.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KGI9"/>
    <dbReference type="GeneID" id="10545131"/>
    <dbReference type="KEGG" id="pgr:PGTG_08600"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08600"/>
    <dbReference type="eggNOG" id="ENOG502SGYR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_900570_0_0_1"/>
    <dbReference type="InParanoid" id="E3KGI9"/>
    <dbReference type="OrthoDB" id="3358442at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016592">
        <property type="term" value="C:mediator complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3490">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019404">
        <property type="entry name" value="Mediator_Med11"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10280">
        <property type="entry name" value="Med11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0010">Activator</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="4" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="87"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU364147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP83414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="309" mass="33386" checksum="D943F9C2902AE33F" modified="2011-01-11" version="1">MSSPDSSPSSASSDSSSSIAFDPNTLTQQPTQANLPAATNPELLLNNPLPSSLNPTIESNSQSTAFTLSGFTNNNLQHSQNTDLTSLANTQSTTNNPSQPISKTSHPTAPSRGWMSQRKGKAIHIDDDHPSLRTELNGEFESDQSEQTITSTSSQRIVELGIVEEQLSQLLNLASDALGSLGPSLDQNESTNTATETFSSSIHQYFELLNKIQLGLRTSMSHLRVSRVSTRILFEPAHVSIPNCPVGLGDLKLMANPTFNQNTLNHPFTKEGQEAGSSALSLGSLVAERNAWQDLVESLEFIKAQRTRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGV4</accession>
    <name>E3KGV4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Blue (type 1) copper domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_08715</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Cu(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29036"/>
        </cofactor>
        <text evidence="3">The crystal structure with reduced Cu(1+) has also been determined.</text>
    </comment>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83529.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327948.2">
        <property type="nucleotide sequence ID" value="XM_003327900.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KGV4"/>
    <dbReference type="STRING" id="418459.E3KGV4"/>
    <dbReference type="GeneID" id="10532211"/>
    <dbReference type="KEGG" id="pgr:PGTG_08715"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08715"/>
    <dbReference type="eggNOG" id="ENOG502S40X">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1058218_0_0_1"/>
    <dbReference type="InParanoid" id="E3KGV4"/>
    <dbReference type="OrthoDB" id="1921208at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00920">
        <property type="entry name" value="Cupredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.420">
        <property type="entry name" value="Cupredoxins - blue copper proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000923">
        <property type="entry name" value="BlueCu_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008972">
        <property type="entry name" value="Cupredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002387">
        <property type="entry name" value="Plastocyanin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052953">
        <property type="entry name" value="Ser-rich/MCO-related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34883:SF15">
        <property type="entry name" value="EXTRACELLULAR SERINE-RICH PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34883">
        <property type="entry name" value="SERINE-RICH PROTEIN, PUTATIVE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00127">
        <property type="entry name" value="Copper-bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00157">
        <property type="entry name" value="PLASTOCYANIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49503">
        <property type="entry name" value="Cupredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0186">Copper</keyword>
    <keyword evidence="1 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173883" description="Blue (type 1) copper domain-containing protein" evidence="5">
        <location>
            <begin position="24"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="domain" description="Blue (type 1) copper" evidence="6">
        <location>
            <begin position="52"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="166"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="36"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="197"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="238"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Cu cation</name>
            <dbReference type="ChEBI" id="CHEBI:23378"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR602387-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP83529.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="297" mass="31158" checksum="7BD15946FC32AC8E" modified="2012-04-18" version="2">MSKLFHFLLVFVTWASIVSPLSSQGPPPPPPEKKTNPGQPSNIPQGKNFVIKVGNEEAAKIFSPATIQAQVGDMVTFEFHPKAHTVAQASFDKPCEVLASPEGNGFNSGKQTVELKTPNSNLPRWTMQITKPETIWFYCDPHCGLGMVGAINPPTSGEKTFEKYVENSKKAGSKPKNLRAGSAAAPGDPALRLGENPGDKNAGRLADKAGGQTTDLAGAKPGGQSGTLQRGPPKTANEVATLNANSASVDGKDPSTLASKGKDGGSGAASYFMEKRRAILSSVSLSMILPGVLTTIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KH18</accession>
    <name>E3KH18_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">CxC6 like cysteine cluster associated with KDZ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09306</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83593.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328012.2">
        <property type="nucleotide sequence ID" value="XM_003327964.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KH18"/>
    <dbReference type="STRING" id="418459.E3KH18"/>
    <dbReference type="GeneID" id="10532277"/>
    <dbReference type="KEGG" id="pgr:PGTG_09306"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09306"/>
    <dbReference type="HOGENOM" id="CLU_088670_0_0_1"/>
    <dbReference type="InParanoid" id="E3KH18"/>
    <dbReference type="OrthoDB" id="3055037at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040898">
        <property type="entry name" value="CxC6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18721">
        <property type="entry name" value="CxC6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxC6 like cysteine cluster associated with KDZ" evidence="1">
        <location>
            <begin position="101"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF18721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83593.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="274" mass="30793" checksum="63740E12E32BF2B1" modified="2012-04-18" version="2">MSQMLQRIVNLYNSTFMGDHTPPLFTPQQLFAPRMSVEVCKDGMDIDTLIFNYTSRGEVLKVPSSGLDRVRYNEAKKQCSSWIAAEGTAHKNHSCVVRAVVTDGLTIGHWRCSASAAQLEKLAQEAGHPAPDGPCRRTLDTVRNRYCSFHQPFLEGICQAQPCIQPALPNKKTCGLQSHLDAAKKFGTRAQTSTWIQLSIWKTGQGKLKISIRCSKRMNLIGLRKAEDLVKKVLQKSQHCQERELTMINWLWLLAVSYLPAKQCSTLSHLVQLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHY1</accession>
    <name>E3KHY1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09619</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EFP83906.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328325.2">
        <property type="nucleotide sequence ID" value="XM_003328277.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHY1"/>
    <dbReference type="GeneID" id="10532596"/>
    <dbReference type="KEGG" id="pgr:PGTG_09619"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09619"/>
    <dbReference type="HOGENOM" id="CLU_050896_1_0_1"/>
    <dbReference type="InParanoid" id="E3KHY1"/>
    <dbReference type="OrthoDB" id="2511178at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="351"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="403"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="56"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="248"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="365"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="404"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83906.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="432" mass="48847" checksum="C93CDD5A24564491" modified="2012-04-18" version="2">MEEISRLSNNNENSNAENSRNESQSLHQNPEEETDLLDYSEEMTRDTSSAPPPPSGFASLGSSLPSITDTATRRQAAEVSQSERGISPALINSRATSLALETMSNRSTPVPRPIDRLTFPVHMNTETGRQVLTPIPRQSRQTQFPVLEQPLAPQDEDTIMDNTELETVINLFNDQWNMFVQARENNNPRLMRVALIQAISSQEEIRLLAGGAEMLRISENWIAREELADLERSQLANQNQPASRLAITQTPHEHTVSPSPTPQRRSIRQPSDVTFLGTGPVQQGSNHMPPPPPPSTQPPSAIVRQNTAQQVRQYNQQPPPQYRERGYYQQPPHLETTVQTQAPQYQQQTGVVPNYAPQRPHRPPQQRSWRGSGRNWRRPQDQTSRLLEVGDYLMRAERIAGRVFRTRGRGRARGRGRGQPPRQEEPPAQNHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KI20</accession>
    <name>E3KI20_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09658</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EFP83945.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328364.2">
        <property type="nucleotide sequence ID" value="XM_003328316.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KI20"/>
    <dbReference type="GeneID" id="10532637"/>
    <dbReference type="KEGG" id="pgr:PGTG_09658"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09658"/>
    <dbReference type="HOGENOM" id="CLU_752568_0_0_1"/>
    <dbReference type="InParanoid" id="E3KI20"/>
    <dbReference type="OrthoDB" id="10281162at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172637" description="Secreted protein" evidence="1">
        <location>
            <begin position="28"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83945.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="368" mass="41167" checksum="B5C14AE516221F94" modified="2012-04-18" version="2">MGLPLSPPRWVIAQVIIYLALCSSLQCGIPKTLSTIPEDRAITVFREAPYVTPEAFYRPAYVTPEINPGSRVAIAKNKIVDALFKEAKKGRAPSIWEIKHVKENLVPDAIQEIFGRAQQSPHSDETINALIEGLEKSLMGKGYTPNILKAAIAFQQGLLSLSTRTWKDSSKMADTIESMLQRNGDYIRQLRRNDRVGEVLQSTLGTSEMLDHKLSPNYATIDGLGKNIEFDRGLSEILQQIRKLAEADSPRSASLYKQIPKQRTAVPISRVVSDVKSPSARPQTLIKQLREILSPRTPVSFQTKTAALLLLHHTAHWHLDQTPRILTDHSEMLELRFLAANALKHLSDSEDHTLIFPDHRALINASLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJC5</accession>
    <name>E3KJC5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178290">
        <property type="protein sequence ID" value="EFP84400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328819.1">
        <property type="nucleotide sequence ID" value="XM_003328771.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJC5"/>
    <dbReference type="GeneID" id="10545828"/>
    <dbReference type="KEGG" id="pgr:PGTG_10120"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10120"/>
    <dbReference type="HOGENOM" id="CLU_2110166_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJC5"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="34"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="115" mass="13686" checksum="15C49377F1007BE0" modified="2011-01-11" version="1">MDELAANLLHELDELVFNLLHGLDELVFNLLRELVWVSCVSWFLTSCVSWVSWYIYLPHELDELAANLLHELDELGLKFWVSCVSWFLTSCVSWVSWYMYPPRELDELEYLRWAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJM6</accession>
    <name>E3KJM6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_10221</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178290">
        <property type="protein sequence ID" value="EFP84501.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328920.2">
        <property type="nucleotide sequence ID" value="XM_003328872.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJM6"/>
    <dbReference type="STRING" id="418459.E3KJM6"/>
    <dbReference type="GeneID" id="10546011"/>
    <dbReference type="KEGG" id="pgr:PGTG_10221"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10221"/>
    <dbReference type="HOGENOM" id="CLU_3051432_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJM6"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP84501.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="54" mass="6061" checksum="AD7F8C04EAB0607E" modified="2012-04-18" version="2">MTYILDASRVDEWDQNVLIPAQNILSAFLNYDHLPSPKSISSIADSILSELQVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KL10</accession>
    <name>E3KL10_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11154</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP84985.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329404.2">
        <property type="nucleotide sequence ID" value="XM_003329356.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KL10"/>
    <dbReference type="GeneID" id="10526957"/>
    <dbReference type="KEGG" id="pgr:PGTG_11154"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11154"/>
    <dbReference type="HOGENOM" id="CLU_573821_0_0_1"/>
    <dbReference type="InParanoid" id="E3KL10"/>
    <dbReference type="OrthoDB" id="2510212at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84985.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="422" mass="46766" checksum="EC8C094777EB49AF" modified="2012-04-18" version="2">MNNPNPHYHYHQTANYTTPSRNPPQPRNSGTPSGRHVPPNATGGNPLGPHRVTPTDHRFHPHNHQMSPPGNDNQGSPRRPATDGRMPRTQEGPDLGDYNDGTDLSDLMDTGLLPALEGEGPRGAGAYEDPPLPPPLELGQGDVPPLQLNMPQQRPEQQVPPDLQPQPPVQTTEAEVMRRFMDSPDGMATILGALIQRGGAASNPVAITSRHIYGPVVRAQVLAQPAAWRHLHLPAGLASNTNDAIQNLDQFLRTMVKHERTTLRNLLLIQVRPDPRKRAPGPIPRLFDLLVLIEQGLTPRHETRTRDQVAEWATRPVRLRFALLRLLAVHHYINRPPGQTLSQWEIIDRHLEMLAGPLSPVELQAHIELIIQKDSEYFDGIKRIEDIPPATFHLPTDAEISQEIARLNRSPVVTQEAAPPGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3KL51</accession>
    <name>E3KL51_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF659 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11195</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP85026.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329445.2">
        <property type="nucleotide sequence ID" value="XM_003329397.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KL51"/>
    <dbReference type="GeneID" id="10527003"/>
    <dbReference type="KEGG" id="pgr:PGTG_11195"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11195"/>
    <dbReference type="HOGENOM" id="CLU_516927_0_0_1"/>
    <dbReference type="InParanoid" id="E3KL51"/>
    <dbReference type="OrthoDB" id="2506324at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052717">
        <property type="entry name" value="Vacuolar_transposase_reg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46169">
        <property type="entry name" value="DNA REPLICATION-RELATED ELEMENT FACTOR, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46169:SF15">
        <property type="entry name" value="INNER CENTROMERE PROTEIN A-LIKE ISOFORM X1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="430"/>
            <end position="453"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85026.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="527" mass="59990" checksum="C8A8BC54B7D4D13D" modified="2012-04-18" version="2">MKAQAILNHERYHSFVQFGVHKLHGRFLLLENKHIEESCTRLLSRIYPHKRRSQMTLLDYTLRCRSCSSNPLKGLMYLGLDAWQSPNGFNILGTVGYRLIGEDNGGFELEAMPLDFVRLKQSHTGLYLDETVQLIVEKFGLKEKICGIVTDNASNNKTMIEAIKSYRWPRFKGKTQWIRCFAQILNLIVQETGDSGDDEDDKDSNLSKKDAELVGRLINNDEIELKDEDVNEMSDEDEDDRYTSKSCKESLSKFRAISRKLNKSPNSKALFVELCQDLECASRTQLDVILEWQRDKRHGPSREYHINSNNIDLARDLIEVLQPFYNITLQVSTRGGAQISDVVVFIDQITSHLSTAISKKKDDFPPALRNACRAGLQLTNKYYTLTDCSPLYRVAMVLHPSFKDEYFKLANWNPEWIEEAIRLTREMWETQYKPPPPQPSNSQPTAPRQQTKTQTGVLAGFIMTMGGPSTPSNGGHNNGVRATLMGVFSRWHLMSLAVQQQLLTLSDHSALEGTTFFSKGRGLVCLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLG7</accession>
    <name>E3KLG7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_11311</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP85142.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329561.2">
        <property type="nucleotide sequence ID" value="XM_003329513.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLG7"/>
    <dbReference type="GeneID" id="10527123"/>
    <dbReference type="KEGG" id="pgr:PGTG_11311"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11311"/>
    <dbReference type="HOGENOM" id="CLU_2777167_0_0_1"/>
    <dbReference type="InParanoid" id="E3KLG7"/>
    <dbReference type="OrthoDB" id="10403299at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP85142.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="69" mass="7966" checksum="DCA50AD0D3F39363" modified="2012-04-18" version="2">MKIARISIVATILSGSIYFKAHGIEFKTIHRWEWDSTDEIAATVQAELPTWGQQRWAATLHYAIWPLHC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3KML4</accession>
    <name>E3KML4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11895</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178295">
        <property type="protein sequence ID" value="EFP85539.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329958.2">
        <property type="nucleotide sequence ID" value="XM_003329910.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KML4"/>
    <dbReference type="STRING" id="418459.E3KML4"/>
    <dbReference type="GeneID" id="10544099"/>
    <dbReference type="KEGG" id="pgr:PGTG_11895"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11895"/>
    <dbReference type="HOGENOM" id="CLU_1129541_0_0_1"/>
    <dbReference type="InParanoid" id="E3KML4"/>
    <dbReference type="OrthoDB" id="10346851at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018034">
        <property type="entry name" value="Kri1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14490:SF5">
        <property type="entry name" value="PROTEIN KRI1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14490">
        <property type="entry name" value="ZINC FINGER, ZZ TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05178">
        <property type="entry name" value="Kri1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="148"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="168"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85539.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="246" mass="28113" checksum="1C3D8BB67CB6E48C" modified="2012-04-18" version="2">MNFLHSPNPTVTTHARDTGPSARRKDDSRKLGRDAVKARKDEEKQQRLTCYFIDVLDCNLDDIDLDSDFDPESHDRRMQQVFDNDFYGKTDPDGKPVWDDDIEIDEILPDTAPVQEEINFEPGGDAYDPLAPGGKKLSKKMKRLAKAEKKRGVVQNMEPEKEEGPDHTEEVDDLEGLSPEERKRKLLEAMDEYHKLDCEDMGDSPRLDQVHKFDCCPTKTGGLCLSLDISDKKNKDAKSLLTERYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KP81</accession>
    <name>E3KP81_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12062</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178298">
        <property type="protein sequence ID" value="EFP86106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330525.1">
        <property type="nucleotide sequence ID" value="XM_003330477.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KP81"/>
    <dbReference type="STRING" id="418459.E3KP81"/>
    <dbReference type="GeneID" id="10540112"/>
    <dbReference type="KEGG" id="pgr:PGTG_12062"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12062"/>
    <dbReference type="HOGENOM" id="CLU_1918110_0_0_1"/>
    <dbReference type="InParanoid" id="E3KP81"/>
    <dbReference type="OrthoDB" id="2507429at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="75"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="132" mass="15295" checksum="22D6C4FC12FDC9CF" modified="2011-01-11" version="1">MATPINIVLDPALPSSHARKQQYHYQNGTFFGSDETVSKKKYLVRRRCQETPKETRNEEEQTVIPDEERQREGGEEGDSEDEDESEKQARAPNYLEHEDLQLCTSWLETTEDGQKDTNQTGTAFWETVANHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPL0</accession>
    <name>E3KPL0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RlpA-like protein double-psi beta-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_12201</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178299">
        <property type="protein sequence ID" value="EFP86245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330664.1">
        <property type="nucleotide sequence ID" value="XM_003330616.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KPL0"/>
    <dbReference type="STRING" id="418459.E3KPL0"/>
    <dbReference type="GeneID" id="10540256"/>
    <dbReference type="KEGG" id="pgr:PGTG_12201"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12201"/>
    <dbReference type="HOGENOM" id="CLU_047639_8_0_1"/>
    <dbReference type="InParanoid" id="E3KPL0"/>
    <dbReference type="OrthoDB" id="623670at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22191">
        <property type="entry name" value="DPBB_RlpA_EXP_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051477">
        <property type="entry name" value="Expansin_CellWall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009009">
        <property type="entry name" value="RlpA-like_DPBB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836:SF27">
        <property type="entry name" value="RLPA-LIKE PROTEIN DOUBLE-PSI BETA-BARREL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03330">
        <property type="entry name" value="DPBB_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="domain" description="RlpA-like protein double-psi beta-barrel" evidence="2">
        <location>
            <begin position="11"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP86245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="62" mass="6681" checksum="06AED5AD34AA0E58" modified="2011-01-11" version="1">MSSISLCGKSIRASYGPNSIVVSVVDRCEGCSPNDLDLSPAAFRRLSPLNTGRLHGIRWTFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPM4</accession>
    <name>E3KPM4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RlpA-like protein double-psi beta-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_12215</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178299">
        <property type="protein sequence ID" value="EFP86259.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003330630.2"/>
    </dbReference>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12215"/>
    <dbReference type="eggNOG" id="ENOG502S6X4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_627202_0_0_1"/>
    <dbReference type="InParanoid" id="E3KPM4"/>
    <dbReference type="OrthoDB" id="623670at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22191">
        <property type="entry name" value="DPBB_RlpA_EXP_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051477">
        <property type="entry name" value="Expansin_CellWall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836:SF27">
        <property type="entry name" value="RLPA-LIKE PROTEIN DOUBLE-PSI BETA-BARREL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173784" description="RlpA-like protein double-psi beta-barrel domain-containing protein" evidence="3">
        <location>
            <begin position="26"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="86"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="92"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="133"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="147"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="180"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="289"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP86259.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="438" mass="47871" checksum="2000E8C01238330A" modified="2012-04-18" version="2">MFHLTITPFFSMVALFLIIVDPSISRPLSHQAGVSKSAFSHHRFLRRSTPSLSSSSHHRRSLDEQQDEFLPFGPISLVDDESENFKRSINPDLDEPQDDPSFDSIDQEKDDADKAWQQFVAPTPDEDPDSYPDSFAQDDASDSSCSTGTNASNVTPKNSPKLVASNEKNKVKSPSSQDAGDYKPQKPKDSGSDKPKDSEASKKKEAEAAQQKEAEAAQQKEDEAAKQKEAEAAQQKEAEAAKEKEAEANKEKKAEEDKKKAEEQKKKDAEAAKKQADQANGAEEAKDQQAVQQPSKSQDGTVTDVQPIPGTSVTTGTVHKDHKGDSYSTPSAEVHTGDATYYQPDLGACGLTNTSSDMIVAVSKLLYDSFPSQGGNPNTNQVCGKRIRATYKGKSCEVTVVDRCEGCKHDDLDFTITAFEKLGSKDEGRLHGMTWTFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPN9</accession>
    <name>E3KPN9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_12230</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178299">
        <property type="protein sequence ID" value="EFP86274.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330693.2">
        <property type="nucleotide sequence ID" value="XM_003330645.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KPN9"/>
    <dbReference type="STRING" id="418459.E3KPN9"/>
    <dbReference type="GeneID" id="10540287"/>
    <dbReference type="KEGG" id="pgr:PGTG_12230"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12230"/>
    <dbReference type="HOGENOM" id="CLU_1816744_0_0_1"/>
    <dbReference type="InParanoid" id="E3KPN9"/>
    <dbReference type="OrthoDB" id="10262308at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000956">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1730:FF:000038">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051742">
        <property type="entry name" value="Ribosome_Assembly_uL10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45841:SF1">
        <property type="entry name" value="MRNA TURNOVER PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45841">
        <property type="entry name" value="MRNA TURNOVER PROTEIN 4 MRTO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173599" evidence="3">
        <location>
            <begin position="23"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="33"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP86274.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="142" mass="16413" checksum="8324625F7317A4F3" modified="2012-04-18" version="2">MEIFMCQVSLAVFFNFTTHTLARGTCTHQLDLQGQHTHTPHQETNQRNRRRKRKGKNALFAQVKQASEKFQSVWLFTVDHVWTPYLQDIRSSWKPSQIYMGRNAVMRLGLRSKEEDERKPGLGTTGKLLEGDTGLLLMNIPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPZ9</accession>
    <name>E3KPZ9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="5">30S ribosomal protein S11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_12148</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178299">
        <property type="protein sequence ID" value="EFP86192.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330611.2">
        <property type="nucleotide sequence ID" value="XM_003330563.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KPZ9"/>
    <dbReference type="STRING" id="418459.E3KPZ9"/>
    <dbReference type="GeneID" id="10540207"/>
    <dbReference type="KEGG" id="pgr:PGTG_12148"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12148"/>
    <dbReference type="HOGENOM" id="CLU_2293061_0_0_1"/>
    <dbReference type="InParanoid" id="E3KPZ9"/>
    <dbReference type="OrthoDB" id="1677536at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="entry name" value="Ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_uS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_uS11_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP86192.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="101" mass="11059" checksum="C28EC8529A885AC2" modified="2012-04-18" version="2">MVTERRSCDDTSQPGCNGSSEGRSKRELALRHEIGRSRRCSSVQQVEITAVHIKLCANSESHTKSPGPGRQANTRALARASMYLRRIEEATPVPTGSTHRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQA9</accession>
    <name>E3KQA9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Xylanolytic transcriptional activator regulatory domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_12440</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178300">
        <property type="protein sequence ID" value="EFP86484.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330903.2">
        <property type="nucleotide sequence ID" value="XM_003330855.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQA9"/>
    <dbReference type="STRING" id="418459.E3KQA9"/>
    <dbReference type="GeneID" id="10540499"/>
    <dbReference type="KEGG" id="pgr:PGTG_12440"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12440"/>
    <dbReference type="eggNOG" id="ENOG502RYZ7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_324423_0_0_1"/>
    <dbReference type="InParanoid" id="E3KQA9"/>
    <dbReference type="OrthoDB" id="2428527at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12148">
        <property type="entry name" value="fungal_TF_MHR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007219">
        <property type="entry name" value="XnlR_reg_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47783:SF1">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47783">
        <property type="entry name" value="ZN(II)2CYS6 TRANSCRIPTION FACTOR (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04082">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00906">
        <property type="entry name" value="Fungal_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylanolytic transcriptional activator regulatory" evidence="3">
        <location>
            <begin position="209"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="645"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="800"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="809"/>
            <end position="822"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP86484.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="890" mass="98077" checksum="FD3463A77DC79093" modified="2012-04-18" version="2">MTKINPPQLVDCHILTHRSFLKIIHPLNCLIQLGPTAIAPGAPFREISLKLVRESGSKVPQTARPTNLDRMVPPTAAVSTPNSSQLNCTPAQLAADFGHDSSFNLTEEQFRRNADVFYDHMGSYLGCIRKEDLLQSLSQGKASEALKLAISALAECIRPSSTTVDYAEICSRRAKLLCLPHLSLPSLDTTFTLLLLAYCEFGREKDSGLWMWSGLAFRMATDLGLHKSSSAIQEPSSVSSPTATSWDQSEAGLRQRIFWSCYHLDRLISSGTGRVATLLDSEIEIDLPSLQAITSEDPLHHHQLLPSPSQEALLSPFHHLTQILILVGKISDAFNRSKLASHSSSSPPSPSSEPEAKTLEHFRTELHDFYTRLPPVLHFTVDIARRHISVKNGPAFLLLHLWFHSLVIITHNPNSVGPGWSSLDVGPMDPLLLEISQGSVRTISDMIAFANVVDPDIVPSLPFASQPILIAGLASIPSQSSQPPNQRPIQAADWREFEICSAALQKMQTRWRGVGWLVSTLHSRARNEQDVDLSSSGGAEVSTADNSLMNKLLVRKAEPELLNPICFSHSNESHHFVGLSATGTFHEPASGRLSLVHQPEICCSSSEPTDFTTHNEEQKWTETGAMGQPVVNQLGGLKLTVKTEGEPVQGSLSGKKHQEHSADLGPTLDSTDYNLQPCPAIKPSHQAGEKFIGAVVGKEDVLSPGPQASSASSYWVTQPIPQIFWPNLPSLEVLNHHKPGGPTYTGLTDENHQVDKDSAVTDQPITLSNTSQTDGGCAMIRVKEIENFQQSNSNVLNPFILHADNHGDNHHHHHQHQHHPHLHPLQQQQQQPPPPLEQQQQQLPDHILGLPEFFEHQSHPQQSPSPVFAKEVWDHLFRGEVGIWSSFHHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KR52</accession>
    <name>E3KR52_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13159</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178302">
        <property type="protein sequence ID" value="EFP86777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331196.1">
        <property type="nucleotide sequence ID" value="XM_003331148.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KR52"/>
    <dbReference type="GeneID" id="10541364"/>
    <dbReference type="KEGG" id="pgr:PGTG_13159"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13159"/>
    <dbReference type="HOGENOM" id="CLU_522888_0_0_1"/>
    <dbReference type="InParanoid" id="E3KR52"/>
    <dbReference type="OrthoDB" id="2504536at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173811" evidence="1">
        <location>
            <begin position="20"/>
            <end position="495"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="495" mass="55338" checksum="A9B1CAF4DF2A5521" modified="2011-01-11" version="1">MVIAISLGILISATEVGLASEAIGMTKSTDRLDLSPCAQQIFESNRGGAADHLKASKIRYDKLHAKIQKSITDGNFIHVEDGESDDLWQNILGIMRGLVPTKVFLHGGYWKVREKCAHVMWDYFCAVFGIKKPEILLLHANKKGSFQPFDNAEGNGLLEIEDIEDLKKSSLDLTKEEYKEKIDQAGNSLRETLKNNDFTTIALKTAPAGLVDIIEEFKDKVAIIWTGPVERKPKSSSWVIKYNYYQAPVEGDRLLAMGVPIIITSPWIANARMNAIVDKQFMSAYRALLPKDTSFLPTDLSFPGFKNLANISVEQSAKFSQYIFSLADGLREKMFDLNKTLTKQLQSDKEDIHHQNLSKGDLEKALKKAEDTYFSQTLLAKRWEKLSSLNTENKIFREFCPVDHAVQVVSDPALKECVGEVVEVEMKRPDPAGDKLAIEVKPKLGSDIFIISQMFSPPLEDQIQHVIDWMAGGEKTKDLLTASSTQTPKGIPSEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3KS02</accession>
    <name>E3KS02_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13296</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EFP87077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331496.1">
        <property type="nucleotide sequence ID" value="XM_003331448.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KS02"/>
    <dbReference type="GeneID" id="10542319"/>
    <dbReference type="KEGG" id="pgr:PGTG_13296"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13296"/>
    <dbReference type="HOGENOM" id="CLU_1019898_0_0_1"/>
    <dbReference type="InParanoid" id="E3KS02"/>
    <dbReference type="OrthoDB" id="10296449at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="108"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="77"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="237" mass="26371" checksum="D6AE20241CE2C604" modified="2011-01-11" version="1">MSSFFEIPDRFDGQPLSPYSLHSEPSTAAEEEPMLSPDPETFVKAESPDEDAVASPTPASVANSESSCEDGLASSTDIRRRVEDHVPTPPAAFQVNAEGQFPFHYQERYARSPSPPTSSDAPGSGTASSPIRVDTPPPAEPCYVYPPLPDPDHELTPEERGPIWAEIVQEDAVLNARRHIKAFFRSYFELLDGDVLNHPRRSGGYRRLARCRLYENVMEELDRVLETVDQVQMTVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>E3KSP4</accession>
    <name>E3KSP4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PGTG_12914</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="11">Component of the U5 snRNP complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DEAD box helicase family. DDX23/PRP28 subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="DS178305">
        <property type="protein sequence ID" value="EFP87330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331749.1">
        <property type="nucleotide sequence ID" value="XM_003331701.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KSP4"/>
    <dbReference type="FunCoup" id="E3KSP4">
        <property type="interactions" value="648"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KSP4"/>
    <dbReference type="GeneID" id="10537898"/>
    <dbReference type="KEGG" id="pgr:PGTG_12914"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12914"/>
    <dbReference type="eggNOG" id="KOG0333">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003041_11_3_1"/>
    <dbReference type="InParanoid" id="E3KSP4"/>
    <dbReference type="OMA" id="ARDIKHM"/>
    <dbReference type="OrthoDB" id="196131at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17945">
        <property type="entry name" value="DEADc_DDX23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18787">
        <property type="entry name" value="SF2_C_DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000322">
        <property type="entry name" value="probable ATP-dependent RNA helicase DDX23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000629">
        <property type="entry name" value="RNA-helicase_DEAD-box_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014014">
        <property type="entry name" value="RNA_helicase_DEAD_Q_motif"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47958">
        <property type="entry name" value="ATP-DEPENDENT RNA HELICASE DBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00039">
        <property type="entry name" value="DEAD_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51195">
        <property type="entry name" value="Q_MOTIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="8" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DEAD-box RNA helicase Q" evidence="17">
        <location>
            <begin position="398"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="15">
        <location>
            <begin position="429"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="16">
        <location>
            <begin position="652"/>
            <end position="814"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="1"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="269"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="814"/>
            <end position="834"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Q motif" evidence="13">
        <location>
            <begin position="398"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="16"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="43"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="98"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="113"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="147"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="160"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="173"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="184"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00037954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EFP87330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="834" mass="90347" checksum="EA36144D90DB70E0" modified="2011-01-11" version="1">MGMGPSPVVGSNGSSKPASSRASTPNGGPKQPLSIETLLAQQKAEKEAASKPKFLSKEQRAALAIAKREQEVAAEKARLAEAAAKREELERSAREAKYGSYSSRNSSRNGSSEHSYNYNDRRGSSSSRVDMEGVPTAPKALRGKNSTGGTSSSGPGPSSLRNSYKPDSTTNGSSSSADHTSSSMQPPTHPASSVTPDQHGNSQLPSTEPMLGTAPPPVLNQKLLMARYLGQPDNKKRRIRKMSDKKFVFDWAKDEDTALEEVDPLYAITVPSAPPPNHSSNHHGGSHSRGSVPPPTTTAVQLVGRFGLYGNGKLAGIDPAVQTNKSAARELAQRRLDPEDEVLTSRPCEAGSKRSSAVVNELHWSQKPLNAMRDRDWRIFREDFSIAARGGNIPNPMRSWEESKLPLQILEIIDEVGYKEPSPIQRQAIPLGLNNRDLIGIAETGSGKTASFVIPMLTYIGKLPPLTDDNRHLGPYALILAPTRELAQQIEVETNKFALRLGYRCVSIVGGKAMEEQALNMRDGAEIIIATPGRLKDCIERHVLVLGQCTYVVMDEADRMINLGFEEVVNFILDQLPLSNLKPDTEEAEDSSKMTSFVGGVEGFDLTGAKGLYRQTVMFSATMPPAVERLAKKYLRRPAVVTIGVAGQAVDTVDQQVEFLPNEDKKRGRLLEVLNQGHTPPIIVFVNQKKTADQLAKDISRAGWSTTTLHSGKNQEQREAALASLRAGESDILVATDLAGRGIDVPDVGLVVNFQMAGTIEAYVHRIGRTGRAGKVGTAITFLTNDDADVMYDLKQEIMKSPVSKCPPELAKHEAAQSKMSAAMKRRAADLDDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KT13</accession>
    <name>E3KT13_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_13627</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178306">
        <property type="protein sequence ID" value="EFP87399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331818.1">
        <property type="nucleotide sequence ID" value="XM_003331770.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KT13"/>
    <dbReference type="GeneID" id="10538128"/>
    <dbReference type="KEGG" id="pgr:PGTG_13627"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13627"/>
    <dbReference type="HOGENOM" id="CLU_1949883_0_0_1"/>
    <dbReference type="InParanoid" id="E3KT13"/>
    <dbReference type="OrthoDB" id="2507202at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP87399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="129" mass="14519" checksum="1F777B7BA4E4516D" modified="2011-01-11" version="1">MAYHHHKATEEIHDGVMNLPSIPKFGMILFPSNPSLQFVWEMFFDDEAECADILEELLSHRNSARPQLPIPHQLALTLERLGSNGNGASVGRFSRNLSVGRGTLIKVSRRVIQAINEVLTNFIAWPNKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KTB2</accession>
    <name>E3KTB2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_13908</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178307">
        <property type="protein sequence ID" value="EFP87537.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331956.2">
        <property type="nucleotide sequence ID" value="XM_003331908.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KTB2"/>
    <dbReference type="GeneID" id="10538436"/>
    <dbReference type="KEGG" id="pgr:PGTG_13908"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13908"/>
    <dbReference type="HOGENOM" id="CLU_1687541_0_0_1"/>
    <dbReference type="InParanoid" id="E3KTB2"/>
    <dbReference type="OrthoDB" id="10452837at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172188" evidence="2">
        <location>
            <begin position="21"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="108"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP87537.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="172" mass="18177" checksum="60602B510EC8DC51" modified="2012-04-18" version="2">MAFKNLVILAVLMSMMIGSAMPISYPTTRLLSIRGTEQASSLSNGAEQAATASSGGQTKTDDSSMEMPKTQPEPPKTTPLDDRAPTESDESLHNAASKTQKPESPAGSSLSSSNNTSTVQEAFKPSKANLLEADLNGSHLSLIYSLPTRRPLTSNQSNFNSSPIGLQFASFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3KTK3</accession>
    <name>E3KTK3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Signal peptidase complex subunit 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_13496</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the signal peptidase complex (SPC) which catalyzes the cleavage of N-terminal signal sequences from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. Enhances the enzymatic activity of SPC and facilitates the interactions between different components of the translocation site.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SPCS2 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178308">
        <property type="protein sequence ID" value="EFP87710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332129.1">
        <property type="nucleotide sequence ID" value="XM_003332081.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KTK3"/>
    <dbReference type="STRING" id="418459.E3KTK3"/>
    <dbReference type="GeneID" id="10539108"/>
    <dbReference type="KEGG" id="pgr:PGTG_13496"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13496"/>
    <dbReference type="eggNOG" id="KOG4072">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1230168_0_0_1"/>
    <dbReference type="InParanoid" id="E3KTK3"/>
    <dbReference type="OMA" id="KHACDDA"/>
    <dbReference type="OrthoDB" id="29558at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005787">
        <property type="term" value="C:signal peptidase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009582">
        <property type="entry name" value="Spc2/SPCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13085">
        <property type="entry name" value="MICROSOMAL SIGNAL PEPTIDASE 25 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13085:SF0">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06703">
        <property type="entry name" value="SPC25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="87"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="30"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP87710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="227" mass="24873" checksum="4D9A848014330527" modified="2011-01-11" version="1">MAASTRKNKAAADGSPSKHPEKPAAGVEQDSSDNESSQEDDDQTVPVQVNNACITELKNALDDTMKSYLTSKVGFTQDYFCDDVKLGLGWTSNIIAAGISLAGWHFGWEKTQGYTAYCVGAYMVLSLALTVFTSTVEKKKVFVGTKPSKASGTGPDKVTISTEVKPFDPVYQLQLEYETCKKSGPPKKYQVALKPHFKEFFDVDGILDESRWNNYLDNALKTAISKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>E3KW69</accession>
    <name>E3KW69_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_14122</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178314">
        <property type="protein sequence ID" value="EFP88544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332963.1">
        <property type="nucleotide sequence ID" value="XM_003332915.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KW69"/>
    <dbReference type="GeneID" id="10541010"/>
    <dbReference type="KEGG" id="pgr:PGTG_14122"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14122"/>
    <dbReference type="eggNOG" id="ENOG502S04M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_433550_0_0_1"/>
    <dbReference type="InParanoid" id="E3KW69"/>
    <dbReference type="OMA" id="HEMLPCP"/>
    <dbReference type="OrthoDB" id="2392789at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046921">
        <property type="term" value="F:alpha-(1-&gt;6)-fucosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13132">
        <property type="entry name" value="ALPHA- 1,6 -FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13132:SF29">
        <property type="entry name" value="ALPHA-(1,6)-FUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="135"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="611"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP88544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="631" mass="69737" checksum="92F447EAEB5462A5" modified="2011-01-11" version="1">MVGRKTPASINTAYNVLLDLECQEQRRLLDGGQPRVSPGRGIPTQPRLLRPMYATSNVQLSPLLQRTPKTGEDALTPRGVIRSDEIHEKSYGEVAMDDSKLEAGDTTTLIGGRKFDNDDDGDDDWNDPGEGLGRVTFLCLITVALGLLGLLLFMGKAIDTFDETGSGKGLSKLLANHSQESLLATFPFSPSYYLKDCPSSQPQVGHAHPSTPTPHSSMGLSSTFELIDQPSSSLATIQNQTAYSETDQDGKPICEKSLTYLLDDDFGFTFHLNAISLAASLAEKDSRAFFIVDAEWDRGTWRDHFTKLPDPGCAPPPPSEMVGCPRSTRHWILTSSILSAHFTKDFDDNFGDIEDPDQVILEPVAGIIPYSRRSIYDMARSTLMRLFILSETDDQLIHKTKQEIIQSAVWGPSSDHNLPPFISVHIRKGDRHPHDPAHKYDYVPINYYIDNINSTWTRLREADSTLPESPNVYLASDTPLALEQMRALTPASWKFFHLSEAKSPEISMIAHPHEYSQLHFHAHILSERVGYTRGQIIDMAFLGGGWPKPDGSVMLSSTDFEKPLATICTVSSNMCQFAALQLGWTEAFEESKWINLDLPLSVGWMGIEVPAANTGDSNPDGHGHGGAHHSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KXK8</accession>
    <name>E3KXK8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14911</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178319">
        <property type="protein sequence ID" value="EFP89070.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333489.2">
        <property type="nucleotide sequence ID" value="XM_003333441.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KXK8"/>
    <dbReference type="GeneID" id="10546539"/>
    <dbReference type="KEGG" id="pgr:PGTG_14911"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14911"/>
    <dbReference type="HOGENOM" id="CLU_039964_0_0_1"/>
    <dbReference type="InParanoid" id="E3KXK8"/>
    <dbReference type="OrthoDB" id="2496966at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="176"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="274"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="274"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="291"/>
            <end position="301"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89070.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="375" mass="42269" checksum="694591615DAA8F39" modified="2012-04-18" version="2">MLQIGYDLLGRENPPPGYHSASENWRIHGIGPEPTKLRPQFISCTTKFKLYFPRKGNQCVYDELSSNGEFSYLIWAGSIGLQEFYNLAVDKCEKNRVGAGDIIRDAVASGLPRMDWWISLKLRTAPQFNKTAKPRPKINDESAFAAWMQVIIDSGADNTNSYLITARAYKLARQAAQDANASKRPAPGQDHPDQAPTAADFNTGNVLANKLLSIHRPNTKYKRKLPVFIHPTEDNQYIPLTGAVLDIWATALEKSVDGVSLYSPPASIKFEKVSAAKRQRLDDDRPRRKYSPTSDSDSSVLIQPDDNLMRKYVKFVIKPEKRDAVLQILDQHNIVHPKLFKSKTITEEKMSKWGLSDGIVAQLRDNVSRFDKNPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KYV4</accession>
    <name>E3KYV4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_15342</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178322">
        <property type="protein sequence ID" value="EFP89500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333919.1">
        <property type="nucleotide sequence ID" value="XM_003333871.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KYV4"/>
    <dbReference type="GeneID" id="10547468"/>
    <dbReference type="KEGG" id="pgr:PGTG_15342"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15342"/>
    <dbReference type="HOGENOM" id="CLU_1778395_0_0_1"/>
    <dbReference type="InParanoid" id="E3KYV4"/>
    <dbReference type="OrthoDB" id="10511323at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP89500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="146" mass="16205" checksum="4EA217CBDA8B69B5" modified="2011-01-11" version="1">MVGGYPSRIPAICWRIPASRCGFGSGWPPFPQFVGRYPGYPKGYPLPADRYPPADLPPLPSSNNYQSAPSRTQKIQSTNPPKCILLQMLTLPQFFDALIPTPPPLWKVRHTLGDFLHFSAPQHVLLRFVVRGNLLDGLVGNTLTIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZ20</accession>
    <name>E3KZ20_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15694</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178323">
        <property type="protein sequence ID" value="EFP89545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333964.1">
        <property type="nucleotide sequence ID" value="XM_003333916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZ20"/>
    <dbReference type="GeneID" id="10547514"/>
    <dbReference type="KEGG" id="pgr:PGTG_15694"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15694"/>
    <dbReference type="HOGENOM" id="CLU_2307437_0_0_1"/>
    <dbReference type="InParanoid" id="E3KZ20"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="100" mass="11062" checksum="E741E303FCA60205" modified="2011-01-11" version="1">MAARVDQSKEKLDDEREREFAGLSLSMMMMKHQLESNSLYKPEDEPSVGSEIGDDRRMLLLESSTLHVNVELDGQHQGVGIRGDSDGMIKGVNLSGTKQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZ40</accession>
    <name>E3KZ40_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">5-formyltetrahydrofolate cyclo-ligase</fullName>
            <ecNumber evidence="5">6.3.3.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_15714</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(6S)-5-formyl-5,6,7,8-tetrahydrofolate + ATP = (6R)-5,10-methenyltetrahydrofolate + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10488"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57455"/>
            <dbReference type="ChEBI" id="CHEBI:57457"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.2"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the 5-formyltetrahydrofolate cyclo-ligase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.2" evidence="5"/>
    <dbReference type="EMBL" id="DS178323">
        <property type="protein sequence ID" value="EFP89565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333984.1">
        <property type="nucleotide sequence ID" value="XM_003333936.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZ40"/>
    <dbReference type="FunCoup" id="E3KZ40">
        <property type="interactions" value="166"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KZ40"/>
    <dbReference type="GeneID" id="10547536"/>
    <dbReference type="KEGG" id="pgr:PGTG_15714"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15714"/>
    <dbReference type="HOGENOM" id="CLU_066245_2_1_1"/>
    <dbReference type="InParanoid" id="E3KZ40"/>
    <dbReference type="OMA" id="DKWGIPT"/>
    <dbReference type="OrthoDB" id="2015992at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030272">
        <property type="term" value="F:5-formyltetrahydrofolate cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009396">
        <property type="term" value="P:folic acid-containing compound biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035999">
        <property type="term" value="P:tetrahydrofolate interconversion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10420:FF:000023">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10420">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002698">
        <property type="entry name" value="FTHF_cligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024185">
        <property type="entry name" value="FTHF_cligase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23407:SF1">
        <property type="entry name" value="5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23407">
        <property type="entry name" value="ATPASE INHIBITOR/5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01812">
        <property type="entry name" value="5-FTHF_cyc-lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00036539"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP89565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="291" mass="32612" checksum="4BF0FAD89C3CE86C" modified="2011-01-11" version="1">MLGRAVCQIRGTMMANPPSAAWFSSSAATHSSSKSKSTAYQPPPSMGRQKLLIRRQVQEVLKEITSESLVHQGEEVLKHLKSFNHYVDSDRVSCYKSMSSAELPTDSIIKNLLENKKKLFLPYIHSPSEPADPKKTGAHRMEMLELKSWADFQTGLVPVSFSSSSSSPQVFQFDPAKIAGLENALPEGLDLILLPGLAFDRFGNRLGRGKGYYDEYLNAYPSVASRPSITNYPDHLTEKQQSEKSTRRWPILVGMCLREQLLPEKEYIPTQAHDRRIDYLISPDGVLSLAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZX2</accession>
    <name>E3KZX2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_15809</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178326">
        <property type="protein sequence ID" value="EFP89853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334272.1">
        <property type="nucleotide sequence ID" value="XM_003334224.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZX2"/>
    <dbReference type="STRING" id="418459.E3KZX2"/>
    <dbReference type="GeneID" id="10545585"/>
    <dbReference type="KEGG" id="pgr:PGTG_15809"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15809"/>
    <dbReference type="HOGENOM" id="CLU_1556037_0_0_1"/>
    <dbReference type="InParanoid" id="E3KZX2"/>
    <dbReference type="OrthoDB" id="10370862at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002885">
        <property type="entry name" value="PPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00756">
        <property type="entry name" value="PPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP89853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="172" mass="18891" checksum="53C8DDCF01EE83A7" modified="2011-01-11" version="1">MACRQASHLPAGGIPCSLLGGVPPGKLVYVPACREGFLPGKQVYVPACREDSPSDELVHNPARRKGILPEGFLRAHVQEYKARTEGFPIEPRRGSLEGANEVGRRTKLSRATELIARLKSRGKLNQVIYNALLNALFSTREFESGLEVFKLMKLELDSSQNAHPGIELLTSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3L0C0</accession>
    <name>E3L0C0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_16187</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178327">
        <property type="protein sequence ID" value="EFP89899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334318.1">
        <property type="nucleotide sequence ID" value="XM_003334270.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L0C0"/>
    <dbReference type="GeneID" id="10545714"/>
    <dbReference type="KEGG" id="pgr:PGTG_16187"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16187"/>
    <dbReference type="HOGENOM" id="CLU_1687558_0_0_1"/>
    <dbReference type="InParanoid" id="E3L0C0"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP89899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="156" mass="18759" checksum="FF2339789F51DD2D" modified="2011-01-11" version="1">MEKYFHSVKQLRKPTPGLYKEYLDHVMENTQSEKPGSSNTAPANLYWATMWAKDIWDEFNQLNQDYDKMFNDIERSRLLKRIPYESDDYWEKVENQLEKKKILIKSAKDLKLVLNSLNDYIEVSLKEWAWRNDLDQAFFTREFASGKNYILSLFII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>E3L0P4</accession>
    <name>E3L0P4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">methionine--tRNA ligase</fullName>
            <ecNumber evidence="3">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Methionyl-tRNA synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGTG_15946</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Met) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="3"/>
    <dbReference type="EMBL" id="DS178329">
        <property type="protein sequence ID" value="EFP90098.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334517.2">
        <property type="nucleotide sequence ID" value="XM_003334469.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L0P4"/>
    <dbReference type="FunCoup" id="E3L0P4">
        <property type="interactions" value="525"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L0P4"/>
    <dbReference type="GeneID" id="10546076"/>
    <dbReference type="KEGG" id="pgr:PGTG_15946"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15946"/>
    <dbReference type="eggNOG" id="KOG1247">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009710_3_2_1"/>
    <dbReference type="InParanoid" id="E3L0P4"/>
    <dbReference type="OrthoDB" id="5844513at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010494">
        <property type="term" value="C:cytoplasmic stress granule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017102">
        <property type="term" value="C:methionyl glutamyl tRNA synthetase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990825">
        <property type="term" value="F:sequence-specific mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07957">
        <property type="entry name" value="Anticodon_Ia_Met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00814">
        <property type="entry name" value="MetRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.20:FF:000001">
        <property type="entry name" value="Methionine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000037">
        <property type="entry name" value="Methionyl-tRNA synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.20">
        <property type="entry name" value="Methionyl-tRNA synthetase, Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041872">
        <property type="entry name" value="Anticodon_Met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023458">
        <property type="entry name" value="Met-tRNA_ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014758">
        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033911">
        <property type="entry name" value="MetRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029038">
        <property type="entry name" value="MetRS_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000738">
        <property type="entry name" value="WHEP-TRS_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765">
        <property type="entry name" value="METHIONINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765:SF1">
        <property type="entry name" value="METHIONINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19303">
        <property type="entry name" value="Anticodon_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
    <dbReference type="SMART" id="SM00991">
        <property type="entry name" value="WHEP-TRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57770">
        <property type="entry name" value="Methionyl-tRNA synthetase (MetRS), Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51185">
        <property type="entry name" value="WHEP_TRS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="WHEP-TRS" evidence="14">
        <location>
            <begin position="668"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="454"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="619"/>
            <end position="664"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="469"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="640"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EFP90098.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="724" mass="81152" checksum="BF89BD810940503E" modified="2012-04-18" version="2">MTMVTESPGRRKIREEVELSPQVTNAEGKIVPLSDRPNILITSALPYVNNAPHLGNIIGSTLSADVFARYSRTGPVVAQVLYICGTDEYGTTTETKALEEGMTPSDLCDKYHQIHKDSYQWFDIGFDHFGRTTTPKQTEICQEIFTALYDNGHLTEHEIEQVYCEKDARFLADRFVEGTCPKCGSSGARGDQCDTCCQTLDAIELIDKKCKICSSTPISKKSKHLFIDLPKLQKQIESWYNSAKEGHHWSSNGKAFTEAWLRDGLRERCLTRDLKWGVPVPIKGWEDKVMYVWFDAPIGYPSITANYSNDWEKWWKNPKEVTLYQFMGKDNVPFHTILFPAYLMGTGQEWTMLNHISTTEYLQYENVKFSKSNNVGVFGQNARETQVPSEVWRYFLISTRPETNDSQFTWTDFISKANSELLNNLGNFVNRIVKFCNARYHSIVPDPLSWPVVGPPTQSESASKEDESSSTTSTQITSRESFDEEDQAFVGDINGLLTQYIESMESLKLRQALSIVMAISARGNQYLQENKIDYSLLLASPRRCAQIVLVSLNLVYLLSVLVYPSMPSTSELILKQLNAPKRRLSRTFGIDLLPGHTIGDAIYLFRKIDPDRAAFWRKQYGGSKPSGDPAVVESPGESKKAKKKAAHGGKSKGTTPAGPLFSGPKPPEMVEIELAIVAQGERVRQLKAGSMEGEVGMEVSKLLGLKKDLASLIEQLSKTSLAPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3L1N9</accession>
    <name>E3L1N9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16051</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178333">
        <property type="protein sequence ID" value="EFP90464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334883.1">
        <property type="nucleotide sequence ID" value="XM_003334835.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L1N9"/>
    <dbReference type="GeneID" id="10530360"/>
    <dbReference type="KEGG" id="pgr:PGTG_16051"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16051"/>
    <dbReference type="HOGENOM" id="CLU_1094724_0_0_1"/>
    <dbReference type="InParanoid" id="E3L1N9"/>
    <dbReference type="OrthoDB" id="2497063at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="253" mass="28563" checksum="C9E26381E96CF11B" modified="2011-01-11" version="1">MNPPSFADNAPASGDCIVWTEEKPAITWESSLCQNGASPLDEKAKSQVLGYYSSLLSPIEEDLPPKYDSRDRRGQPHILHSLLHTIRFILLDHLVIKVLVVYLLMVYTAGNRSLLSPFAPSNWSNDEGLLRSHSRDFLTVDIYELYDDTDAWSRSILGRVGSDSPLLTYSEMELVGQVEWEVEPETNPDEEFVIETESAEEQVTPPPKVPKTLPLPFRRPSHVFNDLLRLYDQGNHGSTQSSDSRSPLHDTQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3L1X1</accession>
    <name>E3L1X1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_16572</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178334">
        <property type="protein sequence ID" value="EFP90546.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334965.2">
        <property type="nucleotide sequence ID" value="XM_003334917.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L1X1"/>
    <dbReference type="GeneID" id="10530445"/>
    <dbReference type="KEGG" id="pgr:PGTG_16572"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16572"/>
    <dbReference type="eggNOG" id="KOG0254">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012970_2_0_1"/>
    <dbReference type="InParanoid" id="E3L1X1"/>
    <dbReference type="OrthoDB" id="2241241at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23501:SF87">
        <property type="entry name" value="SIDEROPHORE IRON TRANSPORTER 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="116"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="158"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="247"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="332"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="363"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="402"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="444"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="468"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="497"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="605"/>
            <end position="627"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="7">
        <location>
            <begin position="92"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="282"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="633"/>
            <end position="673"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="11"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="52"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="640"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="657"/>
            <end position="673"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP90546.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="673" mass="74656" checksum="2D75437482EC6D8B" modified="2012-04-18" version="2">MDATNKPTDLSPRRNEEEDDLRQERFVTEGGSRRRTTQGQQQYDGPDDDEDDQRRQQDEAEKASPKQFGVLKMEAIIERLRARSSRWGVRSIYIAMYILSTITSTENNSLPLIEPYFLSLFGHHSSLATVGIMTNIAFAVGKPPMSKIMDVFGRAEGVLVAIILYLVGALLTASSTGVIQYGIARTAAALGSQGLQLSQMIIVADTSSLTSRALLTSTITSPWIFTTWIGPVFGSWFLSKGAYGYRAIYLVFGLAVPLCASWLVLVLWLEWRKLNSEASRPSRRISLPTSDHDQEDQPGGIWSPPNSPHAVLYSSRSSSTSLWSEAWEQLDSVGLVLLTLGFGLLLLPLTWSVKEPGVSWFNVERCTFLILGAIVLVCFGVYESKFAKFPVIPPRLFEQRTVLLGSGVCFWHFICQFTYESYFTSFLQVARFLSARDAQYVERSYLFTACVSAIICGLLVKWTRRYKIWLVVGILFHAVGTLLMVRSRKLDNPMTEIVISQVIGGFGGGFTTLASQLGVQAVVAHQDVGISTAVFLTITQIGGAVGSSLAGSIWTSRLESALAQRLPPSEQSNIPKIVGDLRFALTYEGENRTHINEAYVDVQRILNWLGVWALVPCLICALCMENVNLDSKAPKRKAKEGHPQQDDNDHLAHRDSNVSAENAPFLTQENEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3L2L7</accession>
    <name>E3L2L7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_16794</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178337">
        <property type="protein sequence ID" value="EFP90768.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335187.2">
        <property type="nucleotide sequence ID" value="XM_003335139.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L2L7"/>
    <dbReference type="STRING" id="418459.E3L2L7"/>
    <dbReference type="GeneID" id="10530673"/>
    <dbReference type="KEGG" id="pgr:PGTG_16794"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16794"/>
    <dbReference type="HOGENOM" id="CLU_038618_0_0_1"/>
    <dbReference type="InParanoid" id="E3L2L7"/>
    <dbReference type="OrthoDB" id="2509522at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP90768.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="456" mass="51771" checksum="538914697BB76049" modified="2012-04-18" version="2">MVPPSPKKTSLSQLPTEIKLLVIKYLALQTKKDRRGGGVDEIATPRLNLAFVNRSFYQLCSAINWKLISVLHKEGARLDQLIDEILPRQAQHVRALVLGLDTVAGRNTETLDTLTGTLPRVKLINILNLCTSLAGLQIHLDTYEGKFDTMSTPLPNPIPTSIHPVAQLSNLTYFYLDDNTPGTGEEGCFREEYLINLIRNMIHLVHFRIKGHSASSPTCNLYQHGNHIQPVLSPLAVHLASLNSLKIIDLTLSSCFNSSWSKINWKGALEGIVLHRIPQLSFGILHDFCSLFKESLVSLSLCDCPISIDGNGEYTSLDESEQNYVFYLPKLEKLSVFTPYSTEFIYLFRECYNITKINLVANRQIQPSDIKRLIDHDDPVWTHLKSLVVQVVQVDKGEYRPKKITDLVAYGSKVGIKVECAHHSRHLNRLFMDYQMRIRDNRLWRPVQSSENEIEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>E3L2T1</accession>
    <name>E3L2T1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">type I protein arginine methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.319</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_17046</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-arginyl-[protein] + S-adenosyl-L-methionine = N(omega)-methyl-L-arginyl-[protein] + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48100"/>
            <dbReference type="Rhea" id="RHEA-COMP:10532"/>
            <dbReference type="Rhea" id="RHEA-COMP:11990"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29965"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65280"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:48101"/>
        </physiologicalReaction>
    </comment>
    <dbReference type="EC" id="2.1.1.319" evidence="1"/>
    <dbReference type="EMBL" id="DS178338">
        <property type="protein sequence ID" value="EFP90847.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335266.2">
        <property type="nucleotide sequence ID" value="XM_003335218.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L2T1"/>
    <dbReference type="SMR" id="E3L2T1"/>
    <dbReference type="FunCoup" id="E3L2T1">
        <property type="interactions" value="662"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L2T1"/>
    <dbReference type="GeneID" id="10530752"/>
    <dbReference type="KEGG" id="pgr:PGTG_17046"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17046"/>
    <dbReference type="eggNOG" id="KOG1499">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_017375_1_1_1"/>
    <dbReference type="InParanoid" id="E3L2T1"/>
    <dbReference type="OrthoDB" id="7848332at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016274">
        <property type="term" value="F:protein-arginine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035242">
        <property type="term" value="F:protein-arginine omega-N asymmetric methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035241">
        <property type="term" value="F:protein-arginine omega-N monomethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046656">
        <property type="term" value="P:folic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060567">
        <property type="term" value="P:negative regulation of termination of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.70.160.11:FF:000001">
        <property type="entry name" value="Blast:Protein arginine N-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000003">
        <property type="entry name" value="Blast:Protein arginine N-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.160.11">
        <property type="entry name" value="Hnrnp arginine n-methyltransferase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055135">
        <property type="entry name" value="PRMT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006:SF53">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22528">
        <property type="entry name" value="PRMT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51678">
        <property type="entry name" value="SAM_MT_PRMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="7">
        <location>
            <begin position="64"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Protein arginine N-methyltransferase" evidence="8">
        <location>
            <begin position="166"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP90847.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="347" mass="39048" checksum="5F893BE0860537AD" modified="2012-04-18" version="2">MAQANGGQSSSELKTAESLTSKDYYSDSYAHFGIHEEMLKDEVRTLSYRNSMIWNQHLFKDKVVLDVGCGTGILCMFAAKAGAKQVIGIDMSGIIDQAKKIVKANGFEDTIVLIKGKLEDVELPVDKVDIIISEWMGYFLLYESMLDTVLLARDRYLKPGGLMFPDSATLFMAGLEDQDYKEEKINFWDDVYGFDYSCIKEIALREPLVDTVELRAVTTNPCAVRTFDLLTVTKEDLAFEAPFSLEATRQDYVHALLGWFDVSFRACHKPVSFSTGPHAKYTHWKQTVFYLKDTLTVVKGDKIEGTLAVRPNAKNHRDLDIDISYNLAGQQQTASDALSFKMSVVLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3M2</accession>
    <name>E3L3M2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">5-aminolevulinate synthase, mitochondrial</fullName>
            <ecNumber evidence="5">2.3.1.37</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">5-aminolevulinic acid synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Delta-ALA synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Delta-aminolevulinate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PGTG_16892</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the synthesis of 5-aminolevulinate (ALA) from succinyl-CoA and glycine, the first and rate-limiting step in heme biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>succinyl-CoA + glycine + H(+) = 5-aminolevulinate + CO2 + CoA</text>
            <dbReference type="Rhea" id="RHEA:12921"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="2.3.1.37"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from glycine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.37" evidence="5"/>
    <dbReference type="EMBL" id="DS178342">
        <property type="protein sequence ID" value="EFP91147.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335566.2">
        <property type="nucleotide sequence ID" value="XM_003335518.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L3M2"/>
    <dbReference type="FunCoup" id="E3L3M2">
        <property type="interactions" value="309"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L3M2"/>
    <dbReference type="GeneID" id="10528809"/>
    <dbReference type="KEGG" id="pgr:PGTG_16892"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16892"/>
    <dbReference type="eggNOG" id="KOG1360">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015846_6_0_1"/>
    <dbReference type="InParanoid" id="E3L3M2"/>
    <dbReference type="OrthoDB" id="10263824at2759"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00375"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003870">
        <property type="term" value="F:5-aminolevulinate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd06454">
        <property type="entry name" value="KBL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000006">
        <property type="entry name" value="5-aminolevulinate synthase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010961">
        <property type="entry name" value="4pyrrol_synth_NH2levulA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050087">
        <property type="entry name" value="AON_synthase_class-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01821">
        <property type="entry name" value="5aminolev_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693:SF102">
        <property type="entry name" value="2-AMINO-3-KETOBUTYRATE COENZYME A LIGASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693">
        <property type="entry name" value="CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="8" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="16">
        <location>
            <begin position="172"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="15">
        <location>
            <begin position="584"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="15">
        <location>
            <begin position="588"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="15">
        <location>
            <begin position="603"/>
            <end position="621"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00019560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00032773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EFP91147.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="654" mass="72548" checksum="E84EF1B6460A0C5D" modified="2012-04-18" version="2">MDAKLASSQLRIYKQMCPFLGRTSPLSLRKMSTHSVMGVTRLRLRAQACPVLGPLLSLDPNPITQLNNRQPHHRHYAKPANMDIAIKNIYKKPIQSQQCPSAAKDDDYHHHLINKRDDHHHHHPNQELFDYEKFYNNELEKKHQDKSYRYFNNINRLAAKFPIAHTAEPKEEVTVWCSNDYLGMSKHPLVLESMKNTLTKYGAGAGGTRNIAGNAALHLSLESTLADLHRKESALVFSSCYVANDATLSTLGSKLPGCVIFSDSSNHASMIQGIKHSRAEKVIFQHNDLVDLEAKLASYPKDRPKIIAFESVYSMCGSVGPIEAICDLAEKYGAITFLDEVHAIGMYGPRGAGVAEHCDFEAQAKGGKKGSVMDRIDIITATLGKAYGVVGGYIAGSSSLVDMVRSYAPGFIFTTSLPPAIVSGALTAVKYQMNNLDDRRLQQLNTIELKDKLVNQFDLPVMNNPSHIVPILIGDPAKAKMASDLLLSKHSIYVQSINYPTVPKGEERLRITPTPGHTVEQQAKLIEALDSIWKELNLNRTEDWKRLGGRAGVGVPEPTAGNPQREERIWSDQQLCLVDGTHPIRIGRHQDDPHLDPAKDYRPNNQQSYLLSPQDQGPASFESELNFSHTLDRAPAANSVPVQNSFAKDQQAVV</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2011-05-03" modified="2025-04-09" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3L408</accession>
    <name>RRG9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName>Required for respiratory growth protein 9, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name type="primary">RRG9</name>
        <name type="ORF">PGTG_17140</name>
    </gene>
    <organism>
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>GENOME REANNOTATION</scope>
        <source>
            <strain>CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for respiratory activity and maintenance and expression of the mitochondrial genome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the RRG9 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178344">
        <property type="protein sequence ID" value="EFP91283.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335702.2">
        <property type="nucleotide sequence ID" value="XM_003335654.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L408"/>
    <dbReference type="SMR" id="E3L408"/>
    <dbReference type="STRING" id="418459.E3L408"/>
    <dbReference type="EnsemblFungi" id="EFP91283">
        <property type="protein sequence ID" value="EFP91283"/>
        <property type="gene ID" value="PGTG_17140"/>
    </dbReference>
    <dbReference type="GeneID" id="10529031"/>
    <dbReference type="KEGG" id="pgr:PGTG_17140"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17140"/>
    <dbReference type="eggNOG" id="ENOG502S7IA">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1355255_0_0_1"/>
    <dbReference type="InParanoid" id="E3L408"/>
    <dbReference type="OrthoDB" id="5578174at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010487">
        <property type="entry name" value="NGRN/Rrg9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13475">
        <property type="entry name" value="NEUGRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13475:SF3">
        <property type="entry name" value="NEUGRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06413">
        <property type="entry name" value="Neugrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0496">Mitochondrion</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <keyword id="KW-0809">Transit peptide</keyword>
    <feature type="transit peptide" description="Mitochondrion" evidence="2">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_0000407961" description="Required for respiratory growth protein 9, mitochondrial">
        <location>
            <begin position="18"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="133"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="58"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="133"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="177"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000250" key="1"/>
    <evidence type="ECO:0000255" key="2"/>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000305" key="4"/>
    <sequence length="207" mass="23443" checksum="225AB9A6501C21BE" modified="2012-10-03" version="2" precursor="true">MNSSTIRRSIGLTGSLARQKRVNRSSPLPAAQDPQAGPSGSKAVTEEAPENRLFNHSQEQKDEDKPQWLIHKEALRRKFPDGWNPPKKISRPSMALLRTLHQTDPNQFSLSILSEKFKISPEAVRRILRSKWEPNQETAKKTEQRQQTVGAAHSSDGWVHHEKLETDQIPLNLAHTLQSSLPSSSKISSSSFPRKSQPRSSYNHFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3L4P3</accession>
    <name>E3L4P3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glutamine amidotransferase type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_17572</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178348">
        <property type="protein sequence ID" value="EFP91518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335937.1">
        <property type="nucleotide sequence ID" value="XM_003335889.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L4P3"/>
    <dbReference type="STRING" id="418459.E3L4P3"/>
    <dbReference type="GeneID" id="10529519"/>
    <dbReference type="KEGG" id="pgr:PGTG_17572"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17572"/>
    <dbReference type="eggNOG" id="KOG1268">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042555_4_0_1"/>
    <dbReference type="InParanoid" id="E3L4P3"/>
    <dbReference type="OMA" id="SVHPAWN"/>
    <dbReference type="OrthoDB" id="14446at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061672">
        <property type="term" value="C:glutathione hydrolase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008242">
        <property type="term" value="F:omega peptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01908">
        <property type="entry name" value="YafJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000060">
        <property type="entry name" value="Related to DUG3-probable glutamine amidotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026869">
        <property type="entry name" value="EgtC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052373">
        <property type="entry name" value="Gamma-glu_amide_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43187">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE DUG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43187:SF1">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE DUG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13230">
        <property type="entry name" value="GATase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="2">
        <location>
            <begin position="2"/>
            <end position="353"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP91518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="353" mass="39555" checksum="1640E5A8E6F4CEA2" modified="2011-01-11" version="1">MCRLLIFKGEPIVLAHLITKPSHSIINQAFDSRLRLDARPVNADGFGVGWYDPDVENSTPCVFQAITPAWSNRNLHRLAEKICSPLVFAHVRASTSGALSEENCHPWIYGSLLWMHNGHIAGFKNFKRELQSGLFDEFFHFPQGNTDSEWAFALFLNELSKVADPKSVSFPYTLLKETMLQTIKRIRELCIQAGISEPSLLNFAVTDGQSVVATRYVNSSTDEAASLYFSTGTSFEAASALDGGPAINSEECHGPLEAAQYRMKKCDKRERIVLIASEPLTFEKTDWLEIPCQTVIIITPKMNVLQFPIIDEFFQHTLTPNRSSDYVIGKGLHYLPVSASPSKICPGKQQWRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3L4X1</accession>
    <name>E3L4X1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17650</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178349">
        <property type="protein sequence ID" value="EFP91596.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336015.2">
        <property type="nucleotide sequence ID" value="XM_003335967.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L4X1"/>
    <dbReference type="GeneID" id="10531533"/>
    <dbReference type="KEGG" id="pgr:PGTG_17650"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17650"/>
    <dbReference type="HOGENOM" id="CLU_364907_0_0_1"/>
    <dbReference type="InParanoid" id="E3L4X1"/>
    <dbReference type="OrthoDB" id="2516525at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="159"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="617"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="61"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="629"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91596.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="772" mass="88447" checksum="8CCD706656875856" modified="2012-04-18" version="2">MAIRDGEKSSERTSPLDRGMDLDHPGSSKEKQHISSIGPAIEPDVSRESKIANKDHKTALPPKSSSRASTSPSSPPGPPVQGKRNKRKNSPGDQKELSELYEASIPLLSLREWIFPGDKQVSITEGKEKAIENTEKLKLTTSKETVDYTDVMNANDISNIKGKTKAAKNRTGKKEPQTRPKRKENRLTAVPQKNNRVRVSPELLLAEEKMRRSFYEPYDNKIWGWIFGGTVYYMQENHQAWKYEEKSSTSKFAVKLASEISKTINQGNHPGVDTISKSELNQNALNFAELMLSINLRVLWAFDATLIDKEYLAEHNLIQQWLLGILSQQRSQSAGSSSKTISVEIFPELTLEKVVDNFVGVLSSKKSDILVHRLVKLHDSGPIFLGVASESQLKMTKSIVTLLASYYKLMNSAKWSALFKDHGAYLGHISKIQTEHYNCHSQRIATPKSFMVAMKLLPWKDKMEKIDQSTRKALITSMRVSTSSKRDDRVQPFDTGRPAIVGDEVWASISLIEREEWCIIKADFKQLNLAHSRYQPDKNQYLSPALKQYVSHQRNLAGVEYLVRLLWVLNSRMIEVLGYHLRDPEYYEEQDHIQREMFGILNEPTNQEGLLEALKEKAQTKAKKNQAKPPEETPEPKDDSRSSQEELVANLIFSGLRNKETQRRYETTFRTIERVSRYEIKMTQAVLGMIKYHYKKKNIVKWYNMFKDDAGFMLGFEKLAVRLRNPHTYREFVDVSMPSMRGMQLLPWESTMFSTGAQRLTASFLLRRKQHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3L517</accession>
    <name>E3L517_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Threonine/serine exporter-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_17498</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the ThrE exporter (TC 2.A.79) family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178350">
        <property type="protein sequence ID" value="EFP91642.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336061.2">
        <property type="nucleotide sequence ID" value="XM_003336013.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L517"/>
    <dbReference type="FunCoup" id="E3L517">
        <property type="interactions" value="4"/>
    </dbReference>
    <dbReference type="GeneID" id="10531578"/>
    <dbReference type="KEGG" id="pgr:PGTG_17498"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17498"/>
    <dbReference type="eggNOG" id="ENOG502QPMM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_301901_0_0_1"/>
    <dbReference type="InParanoid" id="E3L517"/>
    <dbReference type="OrthoDB" id="413008at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010619">
        <property type="entry name" value="ThrE-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051361">
        <property type="entry name" value="ThrE/Ser_Exporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024528">
        <property type="entry name" value="ThrE_2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31082">
        <property type="entry name" value="PHEROMONE-REGULATED MEMBRANE PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31082:SF4">
        <property type="entry name" value="PHEROMONE-REGULATED MEMBRANE PROTEIN 10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06738">
        <property type="entry name" value="ThrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12821">
        <property type="entry name" value="ThrE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="627"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="672"/>
            <end position="691"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="703"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="736"/>
            <end position="759"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="834"/>
            <end position="852"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="864"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="889"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="918"/>
            <end position="939"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="951"/>
            <end position="976"/>
        </location>
    </feature>
    <feature type="domain" description="Threonine/serine exporter-like N-terminal" evidence="8">
        <location>
            <begin position="517"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="domain" description="Threonine/Serine exporter ThrE" evidence="9">
        <location>
            <begin position="857"/>
            <end position="974"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="139"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="261"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="384"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="87"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="187"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="210"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="220"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="261"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="308"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="350"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="391"/>
            <end position="413"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00034125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP91642.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="989" mass="108248" checksum="BEB795D66BDFB5BD" modified="2012-04-18" version="2">MSGNNRQSLTERRRSSVRFSEGTFAPSTTGVPDGDALRRSFRDRPSTPAASMLPGAYFPSPSDNDTPFEEAVSDVQRKLPACKEPSVATSQPGFKSNNRLRSASESLHTRPQGSFSQKPKHDFESNKTDLAVEYFPQTSLPTFQPPYTLHASVPQPPSNSIAPPSHQHHPDALFPGRPPLSRANEGIIQNSSPRRSTLDGQPPSYPERTYYNHDYRNSDYTDQALVNSLQPAPPASSGLPQSNTMYDSGEGFRALRDEARRDSDIPHQRGDLESGYPAINLSGVDDPAGRGDPRHRTSQPAKPSLRRATTEDVVEKEPNRKAADIVGSHIKRRRGKSVGNTGLKPPSLMEDFEDVDEFDTPPPASSVKGGVLSHLLELYGNNRAGPIERSGSATSATTYAGGSVATSRQTSIDSRVANRRVPFVSPWDSNEKMYSHSPPKSSEHGDHAKSSKRNRKARDSDGAESDGASVRSTYSVKTFREVVGAVQEKFHHRKTKSRERRATITRHVADILKRQNFILKLAKALMTTGAPSHRLESQLSATARVLEIDAQFVHLPSIVIATFGDADTRTSETHFVKANGGIDLGQLHRVHHVYKSVVHDHTGVGQGSAALHKILNESPLYSPWQRMVIAFFCCGTIAILGFAGSFVDGWVAGAFGSFLAFMQLKASTNQMYSSIFEISVATIVSFISRGLSTTNYFCYESLSSAGVVLILPGYIILCGSLELASKNLIAGSVKMVYAVIYSLFLGFGISIGSDFYYLLDPKARHNTQMQQATQSHVYTLSGSFNGMNGTIPGMIGDFTFSNGTAGVTGEGELHQGRIQCYREPHWEWWRQPASPYWSFLLVPMFSFFLSLWNLQPIWTKQMPVMVIIACIGWVCNFFANKFIFDRSDVVSFLGAFVIGLLGNMYSRFFKGTAFTSMVTGVLFLVPSGIAAAGGLAMTYHSTSGDSYSNGLIIGFRMVQVAIGITVGLFFSSFVVYSFGSGKSSATFTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>E3L5I8</accession>
    <name>E3L5I8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17813</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178352">
        <property type="protein sequence ID" value="EFP91813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336232.1">
        <property type="nucleotide sequence ID" value="XM_003336184.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L5I8"/>
    <dbReference type="GeneID" id="10531758"/>
    <dbReference type="KEGG" id="pgr:PGTG_17813"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17813"/>
    <dbReference type="HOGENOM" id="CLU_2307441_0_0_1"/>
    <dbReference type="InParanoid" id="E3L5I8"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="100" mass="10924" checksum="14401A19673AE1C7" modified="2011-01-11" version="1">MSLNSIAGKSCPPRHVQPNLNRSDYMWNEDTNSDKLKDGCIELLKAKINAKRVDCMGPSNDLTYPTGDESKSNSLMARIASSPSSTFLAPPGYIFCLQAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>E3L602</accession>
    <name>E3L602_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Amino-acid acetyltransferase, mitochondrial</fullName>
            <ecNumber evidence="5">2.3.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Arginine-requiring protein 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Glutamate N-acetyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">N-acetylglutamate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PGTG_18428</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">N-acetylglutamate synthase involved in arginine biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-glutamate + acetyl-CoA = N-acetyl-L-glutamate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.1.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the acetyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.1" evidence="5"/>
    <dbReference type="EMBL" id="DS178355">
        <property type="protein sequence ID" value="EFP91977.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336396.2">
        <property type="nucleotide sequence ID" value="XM_003336348.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L602"/>
    <dbReference type="STRING" id="418459.E3L602"/>
    <dbReference type="GeneID" id="10531929"/>
    <dbReference type="KEGG" id="pgr:PGTG_18428"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18428"/>
    <dbReference type="eggNOG" id="KOG2436">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013088_1_0_1"/>
    <dbReference type="InParanoid" id="E3L602"/>
    <dbReference type="OrthoDB" id="5585968at2759"/>
    <dbReference type="UniPathway" id="UPA00068"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004042">
        <property type="term" value="F:L-glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006592">
        <property type="term" value="P:ornithine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000070">
        <property type="entry name" value="Acetylglutamate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006855">
        <property type="entry name" value="Vertebrate-like_GNAT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342:SF4">
        <property type="entry name" value="AMINO-ACID ACETYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342">
        <property type="entry name" value="N-ACETYLGLUTAMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04768">
        <property type="entry name" value="NAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51731">
        <property type="entry name" value="GNAT_NAGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0809">Transit peptide</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="18">
        <location>
            <begin position="504"/>
            <end position="707"/>
        </location>
    </feature>
    <feature type="domain" description="N-acetyltransferase" evidence="17">
        <location>
            <begin position="507"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="87"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="354"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="695"/>
            <end position="715"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="93"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="16">
        <location>
            <begin position="120"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="133"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="16">
        <location>
            <begin position="699"/>
            <end position="715"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004925"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS51731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EFP91977.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="741" mass="81869" checksum="64B356EC15E5CF2E" modified="2012-04-18" version="2">MNLMTPGLPLPYTMSMMVSRRVPTRRLLNPPLNPNIRSYYLRSSEDDLSSNLKPSADKLQRELILSVLEAHPSARDSRLYLKSFAPTSKKIPKNSPDSSSNSSSNDTFNPGFPTGEAMPTTATLAATTSSSTFVQPNKSNETSIRQTNKNQNRDVAQLILSTPNQHTGLVKIQGPFTDRQLQSIVDGVIYLKTLGLMSIIVLDDEAWHYPSHTSLTASLPHKSKPPGILHTINSSESRRQAIVRETGRLSAMLESRGGQSRPIQEGVLRLTDDRNQDGDGLDLYIDNLDVLKSAIKNQEIPIIPPIAINSSCATICVSADEVVKSLAKGLAAVGKSPNSLNHTIDAVSAYHGQPATVRSPTHQPFDQKNKDRLEEDDNEDNELDLTPLRLMIINREGGIPSPARNGHPHLSINLASEYEFINETFRWGDSHPTSLANLSMAKSCLKYLPNESSAIIVSHRSPKSLIANLITNKPAHSPSLKHSILLNHARDIGLATPTLIRKGLPIKVIRGIDNLDLTKLKDLLEKSFKKTLDHQRYFERLKNSLRFVILAGDYQAAAIVTNEWEEKQESREGSESEDREPIVYLDKFAVLPSLQGEGIVDFLWGALRDESFGLGNLDALNNNGGLSGLGEPVDLVWRSRTDNPVNRWYFERSNGFIKLPPSNPHRLPSTSTSSTKPDFSLFWCTADLPSSIDIKHQHSSSTTATNPSVDPSSSPLIGIIRPTKLQTWCRVINQIPSCWAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3L6B8</accession>
    <name>E3L6B8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4219 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_18183</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178357">
        <property type="protein sequence ID" value="EFP92093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336512.1">
        <property type="nucleotide sequence ID" value="XM_003336464.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L6B8"/>
    <dbReference type="GeneID" id="10532052"/>
    <dbReference type="KEGG" id="pgr:PGTG_18183"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18183"/>
    <dbReference type="eggNOG" id="ENOG502SSQ0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_132218_0_0_1"/>
    <dbReference type="InParanoid" id="E3L6B8"/>
    <dbReference type="OrthoDB" id="2505270at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP92093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="195" mass="22247" checksum="A26172E44AC0C09B" modified="2011-01-11" version="1">MESGSRLESSRYLLTSDNYSTWIVSIEAKLEDIRACELVTGLIKIDEKTTADELAKYSIISRKGYSKIIQNLDATNLALVSTTLPEEEKFNGRALWRLLKNKYAGSDLVARSTALNHFLDLEYTDVGSFCTAIRLANQRLVLANVLRDDQVKIMIMLRKLPRVQYQLFRDIIAMGFATKTFESAVKQRWADTLRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3L816</accession>
    <name>E3L816_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18206</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178370">
        <property type="protein sequence ID" value="EFP92691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337110.1">
        <property type="nucleotide sequence ID" value="XM_003337062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L816"/>
    <dbReference type="GeneID" id="10539188"/>
    <dbReference type="KEGG" id="pgr:PGTG_18206"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18206"/>
    <dbReference type="HOGENOM" id="CLU_035631_0_0_1"/>
    <dbReference type="InParanoid" id="E3L816"/>
    <dbReference type="OrthoDB" id="10282385at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="47"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="473"/>
            <end position="555"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="481"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="555" mass="61455" checksum="5C39018B9010073F" modified="2011-01-11" version="1">MVDANNTRATSKKIKYSSLAKNRSNESSITESLIPKGSAAIETEIQAGSGSTDQRAHSGSGGLANPIGTHEKRLESEGTIRAHSGSLASPIGTQERRLEGTVNNTLYSTLVDTSESRRDRAPVAFLNPMAKAKISINVASDGIEEEEEDNSPAIAVSSVMSDREQMWKRVVTAQNSGDLDLAEMLFKAIGQIGKESSPAVRPAAPIFAIPSNTASGEVTYLGTSTVEQAVPNAKAKTAPQDPEKFFMTEGDLVYAIGTVTNHNSVGFAPLLDENIGKLRSPLPLTIFNRKWQQRAMAYHLDRRQRSDESSADKDKSGGYKGFSFVQEWTQTYAQWTRNHRAFVKTLCDVYKIIKFSKLLATHKDNCDDITEEFGFMVAFRYNMEVRNNTFSHRITEDDVKNVIPDILQRKESVVEQCYNICRNLGELDWEENLYAPGLPYSNHDPLTGYLRPSRSNNGYQQPHNNNMMIHNPMAQGSNWPNHGQGNHLNNNQNSHPKYNNFNNSYNGMGSGNSNQNFHNGNKRPRGGYKGHSYSDNFTGKSDGGQSSGRKGNQNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8Y8</accession>
    <name>E3L8Y8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidase M48 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_18854</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="DS178379">
        <property type="protein sequence ID" value="EFP93013.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337432.2">
        <property type="nucleotide sequence ID" value="XM_003337384.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L8Y8"/>
    <dbReference type="STRING" id="418459.E3L8Y8"/>
    <dbReference type="GeneID" id="10542989"/>
    <dbReference type="KEGG" id="pgr:PGTG_18854"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18854"/>
    <dbReference type="eggNOG" id="KOG2661">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_474974_0_0_1"/>
    <dbReference type="InParanoid" id="E3L8Y8"/>
    <dbReference type="OrthoDB" id="7464992at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07331">
        <property type="entry name" value="M48C_Oma1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051156">
        <property type="entry name" value="Mito/Outer_Membr_Metalloprot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22726">
        <property type="entry name" value="METALLOENDOPEPTIDASE OMA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22726:SF18">
        <property type="entry name" value="PEPTIDASE M48 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="196"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="226"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M48" evidence="9">
        <location>
            <begin position="360"/>
            <end position="537"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="322"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="553"/>
            <end position="574"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="322"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="557"/>
            <end position="574"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP93013.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="574" mass="64894" checksum="8F609ACE07E81EA1" modified="2012-04-18" version="2">MSWKRLNALISSIHTLKKLLHKAGDLIKLAVVDGLSSRRGVYRVTGEKRCIFPPPNCQSTALTLDHQLQPQPQPQPHHQMNNIITHRQLQIPSASFKNTRSLKLCRPYHSAEYLYRPANNKLQYRLPLSCSNLKLNDVTQPLIKTARTNSYSSLLPTHHPQHSIDSTRRSFHSSPPRKDLFGFGVFSCSIPIFKSFLLSLTRGTLVILPFWYRWKLARHFPRASRTLLTIPLFAMCLVIAIGIDQSPRTHRWRLLLMSEAEEMEWSRRRFEDLVSTDGDLIVGPQDPRTQMVKEICDRLMTALDLDSPVSAAAWPRNPEIDEQHHFGRRVEPSRKEIKSSATASSDLLPWKPESSNPEKKLESNDWELFIIDSPRINAFVLPTKKIFVYTGLLELIQNSEEMAAAVIAHEVSHVVERHAVENLGFSALSAVVFDAMRGVSYALTISFPLLSDGLAFCINYLNDVVVQKAYSRKLETEADELGLMIMARAGYNPGAAVKLWNLLNRLEEDQETSHGSSGIGWPETIPWTRTHPTCKDRGLTIQKALPKALKYFHQPQPSSFPSNSTTASTSPSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3L916</accession>
    <name>E3L916_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_18663</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
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    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <source>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
    <sequence length="381" mass="43086" checksum="7C5CCCF3A86A006A" modified="2012-04-18" version="2">MAEYIRKAVATPGTHEISMRELEHAIQAKNIFFLFLHSSRTQVSILNAVQTVGKSLLGSAVILKSDHKDIYEQLGLSSYHPYLLVFKEYQSKPWAKLVLEEVSPKVEPNPLLRHHKLTMDLRHWIAVQSIPVLDELDQRNYFRVLNSGAKKLVVLACISGIVPSRDGINLERSNPRSVELKDEMKDWAIQWRKSQEARGVEDLSVDWVWVNSDVWADWLAHSYGVNLPRPTEDPRESSMIIIVDAKHHRYFDRQENGQDIEFKPSSVFQTLLAVEMGKLSGRLAGTVVSRMKWKIFHFKNAYGHLFAPHWLFFWAVLFGIIGFSYKSYRASHARGAISFSPISPQMSKAFSSAVGLGSPDIKSPGLGSVFGLASNVPCKAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
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    <name>E3L9G7_PUCGT</name>
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178385">
        <property type="protein sequence ID" value="EFP93192.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003337563.2"/>
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    <dbReference type="AlphaFoldDB" id="E3L9G7"/>
    <dbReference type="GeneID" id="10543342"/>
    <dbReference type="KEGG" id="pgr:PGTG_19431"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19431"/>
    <dbReference type="HOGENOM" id="CLU_044887_0_0_1"/>
    <dbReference type="InParanoid" id="E3L9G7"/>
    <dbReference type="OrthoDB" id="2499104at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="437"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP93192.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="485" mass="54951" checksum="2A1198C242ADDF03" modified="2012-04-18" version="2">MSNLQRRLAGEDINLSTIPPGVNPFDYVTGIITPRYFIPLSISATAFLVVFFIFHLLISIFCLVLLLLPYFRGFKQSQWLFRKLYIKDNSGTNVYNTPLYWVNAGILMTASQLIGSLGAQAFILIQILSADSVRYAIHSQLEPPLGLMFIGEMLTYWSLMHCFLVAIYYDNKTHGDPTKHVTRWSPSPTLINSVFLGFPICIVAATIALFTWLCSAHKLFSTGAGQILDSLKQGSSVWDRLRVSSTESEEELQLTTQLLQITTQITNLRQETSVHLDHVVHCFLILQSVLLVLLWITCIIFISMFWLLVSKYQKSNVSSGSASAKTSFFRRWLWPKISRGAFEEQTVSTTPSLYDMAKKDRQFFHLLVRSLGMIIAMMTMMVLFLLGIVRTVDVVRSPYWRGVAAWLATASGTWSAFPITWQCWRLYKEELSGGSHDSENKHSSCKMDATHQDRFPPRPWDTDSTDIELKFISPAASASPISHEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3L9N1</accession>
    <name>E3L9N1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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    <gene>
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    <organism evidence="2 3">
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            <taxon>Puccinia</taxon>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP93256.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="E3L9N1"/>
    <dbReference type="STRING" id="418459.E3L9N1"/>
    <dbReference type="GeneID" id="10543520"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19303"/>
    <dbReference type="HOGENOM" id="CLU_1866131_0_0_1"/>
    <dbReference type="InParanoid" id="E3L9N1"/>
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    <proteinExistence type="predicted"/>
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        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93256.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="137" mass="13981" checksum="06263B7072A19179" modified="2012-04-18" version="2">MANSLPHSRNKTQRDSSTGNRKGLSAAGNSGSPPGPNNQSRSSVQHIGNSGSGRKANKPPPPQACRALDTGPSLGSSNSLSGSMHAPKGHAQLGVQRKPSIDKNSGGKSMLNPNAGVFQPGALSAIVSRVSYPQFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3NXJ3</accession>
    <name>E3NXJ3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">J domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_20240</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS989945">
        <property type="protein sequence ID" value="EFP94292.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338711.2">
        <property type="nucleotide sequence ID" value="XM_003338663.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NXJ3"/>
    <dbReference type="STRING" id="418459.E3NXJ3"/>
    <dbReference type="GeneID" id="10527426"/>
    <dbReference type="KEGG" id="pgr:PGTG_20240"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20240"/>
    <dbReference type="HOGENOM" id="CLU_1318196_0_0_1"/>
    <dbReference type="InParanoid" id="E3NXJ3"/>
    <dbReference type="OrthoDB" id="10250354at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06257">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.110">
        <property type="entry name" value="DnaJ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052276">
        <property type="entry name" value="Diphthamide-biosynth_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001623">
        <property type="entry name" value="DnaJ_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018253">
        <property type="entry name" value="DnaJ_domain_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036869">
        <property type="entry name" value="J_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44240">
        <property type="entry name" value="DNAJ DOMAIN (PROKARYOTIC HEAT SHOCK PROTEIN)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44240:SF10">
        <property type="entry name" value="J DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00226">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="J" evidence="2">
        <location>
            <begin position="107"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP94292.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="209" mass="23962" checksum="58FE22330F086AFC" modified="2012-04-18" version="2" fragment="single">MASAYIAHLGDLSTLFSFVGWWFLPGYVSSTVLGIFYRIFPARRPKAPERGSTNPDEKPIYNPIHELQKSEYDRRARRHHSIAHGLVIGLYLGYTLIVDYQKQTGSNYYSVLGIQSNSLAFNSTTQSVPENLNTVRTQIIQGDLLDEAGLKSHWRRVARSFHPDKLNPPAGLRTEIEIAEWKGNVEAKFIRMREAYETLNDNTKRWAYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3NXR4</accession>
    <name>E3NXR4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_20319</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS989966">
        <property type="protein sequence ID" value="EFP94363.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338782.2">
        <property type="nucleotide sequence ID" value="XM_003338734.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NXR4"/>
    <dbReference type="STRING" id="418459.E3NXR4"/>
    <dbReference type="GeneID" id="10527571"/>
    <dbReference type="KEGG" id="pgr:PGTG_20319"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20319"/>
    <dbReference type="HOGENOM" id="CLU_055324_2_0_1"/>
    <dbReference type="InParanoid" id="E3NXR4"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023:SF4">
        <property type="entry name" value="GLYCINE-RICH PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="3">
        <location>
            <begin position="25"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="46"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="131"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="57"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="68"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP94363.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="206" mass="23192" checksum="F1E2162173E651AF" modified="2012-04-18" version="2" fragment="single">AHTNLQLACARGVGLRLSTPTKGTTFNNLAAWQKLCYAPKWRAEPRVDQPSTPVPIPSSDSIDPDTSLNENTITPSMRSASSISCPIGGKAAKRRQIKNYQHDELLSQTNELAEVSQEQLTAFNKGNNILLEKNKIMQEKLEIERKKLQLEEEKIAIEKDYCQSETEMNGFKILCDAEDLEEKEAKDVLKIIKDQIKNKWHARAAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPD7</accession>
    <name>H6QPD7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF6589 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20686</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EHS63592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890653.1">
        <property type="nucleotide sequence ID" value="XM_003890604.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPD7"/>
    <dbReference type="GeneID" id="13543167"/>
    <dbReference type="KEGG" id="pgr:PGTG_20686"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20686"/>
    <dbReference type="HOGENOM" id="CLU_1993731_0_0_1"/>
    <dbReference type="InParanoid" id="H6QPD7"/>
    <dbReference type="OrthoDB" id="2507310at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046496">
        <property type="entry name" value="DUF6589"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20231">
        <property type="entry name" value="DUF6589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6589" evidence="1">
        <location>
            <begin position="6"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="125" mass="14381" checksum="76370835316D87DE" modified="2012-04-18" version="1">MSEKEAEEVRKRHSQSKLSNLLVRLHEFSTVVEADRAMKAGDIGRLINIWRMWSVMSQSLPGLTHYATYLPRLVLLLTKVLPEGLSRFFRHSIQWQAPSVSESQGDLVKKLTQVIQTDGIRQRHS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPI1</accession>
    <name>H6QPI1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GS catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_20848</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EHS63866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890561.1">
        <property type="nucleotide sequence ID" value="XM_003890512.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPI1"/>
    <dbReference type="STRING" id="418459.H6QPI1"/>
    <dbReference type="GeneID" id="13543231"/>
    <dbReference type="KEGG" id="pgr:PGTG_20848"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20848"/>
    <dbReference type="eggNOG" id="ENOG502SXV0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_187865_0_0_1"/>
    <dbReference type="InParanoid" id="H6QPI1"/>
    <dbReference type="OrthoDB" id="2502039at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS catalytic" evidence="2">
        <location>
            <begin position="4"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS63866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="91" mass="10419" checksum="770F0C2284615A53" modified="2012-04-18" version="1">MKLTGSRQSTKNKNTVDVNKMTAQDRQAHKITAALPRSLDEALMALEKDTTLSKALGQVFVRAYTTTKITEIERYKALTSEEQKRFELEHY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPU7</accession>
    <name>H6QPU7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glycogen [starch] synthase</fullName>
            <ecNumber evidence="7">2.4.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_20899</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[(1-&gt;4)-alpha-D-glucosyl](n) + UDP-alpha-D-glucose = [(1-&gt;4)-alpha-D-glucosyl](n+1) + UDP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18549"/>
            <dbReference type="Rhea" id="RHEA-COMP:9584"/>
            <dbReference type="Rhea" id="RHEA-COMP:9587"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15444"/>
            <dbReference type="ChEBI" id="CHEBI:58223"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.4.1.11"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="6">
            <dbReference type="Rhea" id="RHEA:18550"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Glycan biosynthesis; glycogen biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the glycosyltransferase 3 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.11" evidence="7"/>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EHS64285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890441.1">
        <property type="nucleotide sequence ID" value="XM_003890392.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPU7"/>
    <dbReference type="STRING" id="418459.H6QPU7"/>
    <dbReference type="GeneID" id="13542989"/>
    <dbReference type="KEGG" id="pgr:PGTG_20899"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20899"/>
    <dbReference type="eggNOG" id="KOG3742">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_866367_0_0_1"/>
    <dbReference type="InParanoid" id="H6QPU7"/>
    <dbReference type="OrthoDB" id="6335297at2759"/>
    <dbReference type="UniPathway" id="UPA00164"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004373">
        <property type="term" value="F:alpha-1,4-glucan glucosyltransferase (UDP-glucose donor) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005978">
        <property type="term" value="P:glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008631">
        <property type="entry name" value="Glycogen_synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10176:SF3">
        <property type="entry name" value="GLYCOGEN [STARCH] SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10176">
        <property type="entry name" value="GLYCOGEN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05693">
        <property type="entry name" value="Glycogen_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0320">Glycogen biosynthesis</keyword>
    <keyword evidence="3 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="23"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="156"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="43"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="156"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="168"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS64285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="321" mass="36246" checksum="64C23CB4C65FF1CF" modified="2012-04-18" version="1">MSESQSHINPLLLSTETAGDTYLVNPLTGTSQDGVSPLHETVSTAAPSTTPSASLHSLAPPPELSYRTRSQLDDAVQKWALEHGYAINKTNITTHNGEDRATGVDKRKPTPLAHAKLHARRRLACTPARQSPSHRTQAWVLWEVFFSFKKKFFQNKKKEKEKKRPTRRPGGIPPGRRYNLAGREDSLQVGEVVQPRRPGDFYCGFPDGVLPNGLNVVKFSAMHEFQNIHAISKQKIHDFVRGHFYGHYDFDLDNTLYFFTAGRYDYRNKGVDMFIESLARLNHKLKTMKYKITVVAFIIMPAATHSYTIEALKGQAVTKQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQ49</accession>
    <name>H6QQ49_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21001"/>
    <dbReference type="HOGENOM" id="CLU_107702_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQ49"/>
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        </location>
    </feature>
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        </location>
    </feature>
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            <end position="118"/>
        </location>
    </feature>
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            <begin position="119"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="144"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="184" mass="19018" checksum="790A61695C15AE54" modified="2012-04-18" version="1">MSANDSDDRSIPDTDLTATASLTAEVRQLKELHGIGNCPSNVRANDHIAYHRNSMRRAWLPEATPNEHSFGQTPSAPASVPTHDGNMGPQPPEDSSHAPSVAVPQTPDNSLNVLSSAYTNTGTLHTSTTETGSGAGNPEDDVTALGQSASGGSSVGANGVVGGPDEEARLACSNAIRMLFWNGS</sequence>
</entry>
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        </lineage>
    </organism>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
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    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQL2"/>
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    <dbReference type="KEGG" id="pgr:PGTG_21172"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21172"/>
    <dbReference type="HOGENOM" id="CLU_2085949_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQL2"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="67"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="117" mass="13087" checksum="71FCA08BC10C05AB" modified="2012-04-18" version="1">MSSCYSTPDYCWISNPVDRDSTLKRARTQQTEHAVVIDRSPLANATEQENYTNQDRSLLGRLSSPIAAASSSQSLPSKPIDKTTYTKTSTNQNSSLHFERSAEAPYTNIQQQPYVKN</sequence>
</entry>
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    <accession>H6QQM3</accession>
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            <fullName evidence="1">Uncharacterized protein</fullName>
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    </protein>
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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            <taxon>Pucciniaceae</taxon>
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    </organism>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EHS62741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21207"/>
    <dbReference type="eggNOG" id="ENOG502QR5Z">
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    </dbReference>
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    <dbReference type="OrthoDB" id="2507073at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS62741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="71" mass="8182" checksum="987B042C8CC8EB85" modified="2012-04-18" version="1">MIVEDECGVNLENFYKTLATVSEVSVEQLDTRGDFSQFISNFRALQSKATHITLRNDLIKHHWDLKGRSDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQV4</accession>
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EHS62883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890118.1">
        <property type="nucleotide sequence ID" value="XM_003890069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQV4"/>
    <dbReference type="GeneID" id="13541515"/>
    <dbReference type="KEGG" id="pgr:PGTG_21255"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21255"/>
    <dbReference type="eggNOG" id="ENOG502SW5T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2575005_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQV4"/>
    <dbReference type="OrthoDB" id="2797670at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS62883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="81" mass="8933" checksum="6DF32F894C2BD2AB" modified="2012-04-18" version="1">MAASDLKSVKLTPANHQICEQLPRGLDELWKPIRDHLVYSPKEVSLDDAIGALEAHEQKQNQDLAAITVAEEAIILLKSFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSS0</accession>
    <name>H6QSS0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21855</name>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
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            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178298">
        <property type="protein sequence ID" value="EHS63811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSS0"/>
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    <dbReference type="KEGG" id="pgr:PGTG_21855"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21855"/>
    <dbReference type="HOGENOM" id="CLU_018307_1_1_1"/>
    <dbReference type="InParanoid" id="H6QSS0"/>
    <dbReference type="OrthoDB" id="2505542at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="202" mass="22690" checksum="6EED7857BE132207" modified="2012-04-18" version="1">MTPEIMSDAVPPASGQEKPANSTRDSARTADGTDMSTAKEWFKAVLKIQHSSIAQAQEDCRQAIEDRRTDRQLFLAAHQASATRIARLEDLLLAMNVKNKVAARPAQPTPGRVDLQKFRTLDGPMFRGPFQETESFLRWIHGVQIFFETKDVSNAADKIKILGNLIAETNLQLFYANKAAGFLTQSWEEFKTRMFDFALPTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>H6QU37</accession>
    <name>H6QU37_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178328">
        <property type="protein sequence ID" value="EHS64449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888976.1">
        <property type="nucleotide sequence ID" value="XM_003888927.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QU37"/>
    <dbReference type="GeneID" id="13541056"/>
    <dbReference type="KEGG" id="pgr:PGTG_22263"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22263"/>
    <dbReference type="HOGENOM" id="CLU_3224815_0_0_1"/>
    <dbReference type="InParanoid" id="H6QU37"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="44" mass="4838" checksum="49E5C695A1D89C88" modified="2012-04-18" version="1">MSTVFLYAVDVSSFCSLAFGSVLLSARMAVFWILSFEDRSVNLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUP7</accession>
    <name>H6QUP7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22478</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178348">
        <property type="protein sequence ID" value="EHS64759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888767.1">
        <property type="nucleotide sequence ID" value="XM_003888718.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUP7"/>
    <dbReference type="GeneID" id="13541635"/>
    <dbReference type="KEGG" id="pgr:PGTG_22478"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22478"/>
    <dbReference type="HOGENOM" id="CLU_144321_0_0_1"/>
    <dbReference type="InParanoid" id="H6QUP7"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003606446" evidence="1">
        <location>
            <begin position="23"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="110" mass="10114" checksum="9B9CF2B02E602F56" modified="2012-04-18" version="1">MRFSFGLLSIVATILSTPEIKAVPTHGYFGRHGGLYGGGYGGGYGGGYGAGYGGYGGGYGGGYGGYGGGYGGGYGGGYGGGYGGGYGGGYGGGGGLGFGLGVGLGVGIGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUX7</accession>
    <name>H6QUX7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22528</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178362">
        <property type="protein sequence ID" value="EHS62590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888689.1">
        <property type="nucleotide sequence ID" value="XM_003888640.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUX7"/>
    <dbReference type="GeneID" id="13542206"/>
    <dbReference type="KEGG" id="pgr:PGTG_22528"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22528"/>
    <dbReference type="HOGENOM" id="CLU_1670245_0_0_1"/>
    <dbReference type="InParanoid" id="H6QUX7"/>
    <dbReference type="OrthoDB" id="2495999at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS62590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="158" mass="17391" checksum="90BF76A01B83C0CC" modified="2012-04-18" version="1">MTSIASSLIRSYRIIQQGLLSAVTRNRTFNPQQVSRGLVAKHHPVVLQTPPSTILSGFFRPLGSLPIAASGTTALPFSSNPHYSGFQSFAHQRTFQSSPFRTFTPEPRMSLQRELDGGKSTRMAHDHAPQPVTVPLVDEIVHDHVAARFAFPAPSVEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUZ5</accession>
    <name>H6QUZ5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22561</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178365">
        <property type="protein sequence ID" value="EHS62608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888671.1">
        <property type="nucleotide sequence ID" value="XM_003888622.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUZ5"/>
    <dbReference type="GeneID" id="13542254"/>
    <dbReference type="KEGG" id="pgr:PGTG_22561"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22561"/>
    <dbReference type="HOGENOM" id="CLU_1240665_0_0_1"/>
    <dbReference type="InParanoid" id="H6QUZ5"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="223" mass="25542" checksum="EDC39D982626F2A1" modified="2012-04-18" version="1">MLGCQWGSPQNARESTSQQTTVRHQIQRMLCLSKKVKLGRPLNSANFDKNQYSTYPSYHAISSAKNPLRANRCWLAAALETLYAVFSPLWLRGINGTVNNLFSYLVQHFTSQETYELNLKGTIQSILNQEQSKISDFSSKNYPDNDVTLNPKLHKSTQPGKLFVVNERRKFTCKAQPDVEQSHPTWQEQYFHVLVISPAMFGENRIPYDDVAKLIQLWQSTGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>H6QV18</accession>
    <name>H6QV18_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22606</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178368">
        <property type="protein sequence ID" value="EHS62631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888654.1">
        <property type="nucleotide sequence ID" value="XM_003888605.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QV18"/>
    <dbReference type="STRING" id="418459.H6QV18"/>
    <dbReference type="GeneID" id="13542281"/>
    <dbReference type="KEGG" id="pgr:PGTG_22606"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22606"/>
    <dbReference type="HOGENOM" id="CLU_396963_0_0_1"/>
    <dbReference type="InParanoid" id="H6QV18"/>
    <dbReference type="OrthoDB" id="10444501at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="100"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="337"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="278"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="399"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="418"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="694" mass="77960" checksum="EB6FEABF461110CE" modified="2012-04-18" version="1">MPAQRVGVRTRTPISIGVRSHTPFREGSVGVCVRRGVECDYHSPRASSSHLSSQTIRPGTPDSRASSQEAICKRNPEPPFKAKSPFEAAPTPRCFEEITFHTLPSRESTPADLEETNRGTRQSCSSSTSQHSSGQQSPHSLRNAPTPPGNHSPSQDDHSEPVGKASLPTDRNKSDAPEESSMPTGREGSESVVSTHREASEEWSISIHCDSGMGRSTGAGPDLTQEFSKDDQSEGEQEEPYNQSDVCHIGSQPNPDSTQDLVPDESQREMSRSQSSSHQHRDRGRKRKSEPPSQWPDPSELSWAQIEAYLDHYNLVHNGGTQIARLVGSYNLLRSTLSKDDEQQPSKQPNARASPAPRLPTINHWWRPLISHRLHLSVFILHVQPVRPKESPALLSNMADRKEPKIRARLKPREELKRLKKTRRRGRPSLTRNHDEGDSINFVPVLPKSSKKRPLADPPPKSARAKKTCPGPDDPSPSDLHTRPQASTSRRGRQQTSRRNSYNVHPSFHDNEEEEPWFVPPHENDRSNPGAPKKRKQHEAAHDSDALRPPGPKQTWAVAADPSASLNWQKSRPARGRQGSTERQRQSSYSVHPSLHDEDELARHEPHAPSRSRRLIPHKLTLHLVLRVRRRSTIQMHIRLLLQGPKESNQTIFLRAPNVQMENNEEALRLALSLLMRKFTGEDTEITEFNATAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>H6QV59</accession>
    <name>H6QV59_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22664</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178376">
        <property type="protein sequence ID" value="EHS62720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888602.1">
        <property type="nucleotide sequence ID" value="XM_003888553.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QV59"/>
    <dbReference type="STRING" id="418459.H6QV59"/>
    <dbReference type="GeneID" id="13542329"/>
    <dbReference type="KEGG" id="pgr:PGTG_22664"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22664"/>
    <dbReference type="HOGENOM" id="CLU_2923740_0_0_1"/>
    <dbReference type="InParanoid" id="H6QV59"/>
    <dbReference type="OrthoDB" id="5286008at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS62720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="61" mass="6613" checksum="C93A36D66AD5822C" modified="2012-04-18" version="1">MYFPHTVIGFSSVDKVKAALEVLGEIQNPKDASSISSAQDSVQDALKSSGYLNWSWPLPSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>H6QVN0</accession>
    <name>H6QVN0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22801</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS989979">
        <property type="protein sequence ID" value="EHS63417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890872.1">
        <property type="nucleotide sequence ID" value="XM_003890823.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QVN0"/>
    <dbReference type="GeneID" id="13541075"/>
    <dbReference type="KEGG" id="pgr:PGTG_22801"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22801"/>
    <dbReference type="HOGENOM" id="CLU_1332518_0_0_1"/>
    <dbReference type="InParanoid" id="H6QVN0"/>
    <dbReference type="OrthoDB" id="10389573at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="102"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="110"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="206" mass="22515" checksum="F0F539E7EB032144" modified="2012-04-18" version="1">MIEKSVYKHNRYNKHTSVLKAFYAARASGECLTVLDPVIDPSIGGASEDRLQAINSEVYEDAPTTAAWCRWEDIDDEPEVHAGQESSDCSIEKLDFSSEASSHSFRSGHASGSNLSDYNSNNKADDEEWDVPIGQGDTGGYESYGDNPTGVSQDEEPGHRRPKGQRNNPWWPYKSKEVPSTAPGTSRRVPAVLFAGTAGTSPLFLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQF1</accession>
    <name>E3JQF1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00186</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74230.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307236.2">
        <property type="nucleotide sequence ID" value="XM_003307188.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQF1"/>
    <dbReference type="STRING" id="418459.E3JQF1"/>
    <dbReference type="GeneID" id="10546428"/>
    <dbReference type="KEGG" id="pgr:PGTG_00186"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00186"/>
    <dbReference type="HOGENOM" id="CLU_033090_3_0_1"/>
    <dbReference type="InParanoid" id="E3JQF1"/>
    <dbReference type="OrthoDB" id="10279573at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP74230.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="429" mass="49011" checksum="9DD5C7180519366C" modified="2012-04-18" version="2">MDTMSNRDNEGVDTPDGDLPPAVKQQGDLVIDKFNSLFGRCDPLNEEHRRNGPMPIENNAFINQLKSGPLSIEDNAFLNRLKSTSDRLDSNLLPLLGDQLIAISLSLKPSELQKEPCSTLKRILEMQEELEQTLCQIRSALDLLPEQEVVPNQTNDQHHQEFKNYRVNGLDQCIRDEVLMEISGFFSSSIMLVRQLGFSTEPRINQMVPAGMDVVHTRYRSEASLRSAKIWLRGSEWETVLVGWRLAMDVMDSVLENSIRHISLIPPNHDLNDRPPFEPLGEQNLEVAESLIPIVKLSRIFLARLSKLEMKHKTLPHYTEMCTHQLQSLHDLPLSVIAKLSCLQSMSIRNDMFDEASTIRFNDMVRRLLALFQSSLLDVVLCVVPLLPDLNGFPHQTYLKTWFVTWNTQFIIATQNLTRLTFSFLNNPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQV3</accession>
    <name>E3JQV3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA repair protein rhp7 treble clef domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_00371</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74415.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307421.2">
        <property type="nucleotide sequence ID" value="XM_003307373.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQV3"/>
    <dbReference type="FunCoup" id="E3JQV3">
        <property type="interactions" value="190"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JQV3"/>
    <dbReference type="GeneID" id="10528147"/>
    <dbReference type="KEGG" id="pgr:PGTG_00371"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00371"/>
    <dbReference type="eggNOG" id="KOG1947">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006598_2_0_1"/>
    <dbReference type="InParanoid" id="E3JQV3"/>
    <dbReference type="OrthoDB" id="421226at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019005">
        <property type="term" value="C:SCF ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050648">
        <property type="entry name" value="F-box_LRR-repeat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006553">
        <property type="entry name" value="Leu-rich_rpt_Cys-con_subtyp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056451">
        <property type="entry name" value="Znf_Tbcl_Rhp7"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13382">
        <property type="entry name" value="MITOCHONDRIAL ATP SYNTHASE COUPLING FACTOR B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23550">
        <property type="entry name" value="zf_Tbcl_Rhp7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00367">
        <property type="entry name" value="LRR_CC"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA repair protein rhp7 treble clef" evidence="2">
        <location>
            <begin position="171"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="210"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="86"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="102"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="131"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP74415.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="641" mass="70580" checksum="B365E46D836AD312" modified="2012-04-18" version="2">MARPSRFNQPTNNDQTPAQRPPSNPAASTSRVNTDSENQNPQPNPTAGPSQPQPSTSGVTGPTSALTSFLKEQGIRARNVNRFTPLRQQASTSTAEPAIPEVAEETPDGPSDPEPDSDDLDDKPAKKQKQKLAQTNQPSTTSKAEASSSKKKPEEPEPSKPKPGKYADRKPGLITTCAECGKRFTVTRYTVSAPGQQGQLCDGCTQELAKNPKSRSNDNHLPVPAPKKRKTTKNQVVRQPWSENSIQTLQMACINLIARNTQSVEELGNIGSVNLEKICQIVCKHRELTPSNLSLFVDAAYCDLKLFDCINLRESHLKSIAYFCPQLEKLTLNLCGHMTGEVLQTYVERLSKLRELELFGPYLIRKHEWLKAGKEIELIQAPQIEGFRLKQSPRFDLECVKALVSSCTNLTSLRLIDIGLLDDKTLDVIAQAGLTKLRDLSIANAGIHNGATGEALTDAGVIGLLESIGGTVERLDISQNKKLTDEVLVGISKCCSKLTYLDLNGLKEVTTKGIQGLFAQFKENEVSGLKHLDVSRCIKIGNEALYSILDHSARTLQYLNLNSVDELRAEALERLAKETIHLEYLDVSFVRDVDDFIIKSLLDHMKHLKSLSVYGNNRVSDLCPMKKGVVIKGQERSIVVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRS9</accession>
    <name>E3JRS9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATP-dependent Clp protease proteolytic subunit</fullName>
            <ecNumber evidence="7">3.4.21.92</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_00169</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the peptidase S14 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.92" evidence="7"/>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307219.1">
        <property type="nucleotide sequence ID" value="XM_003307171.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JRS9"/>
    <dbReference type="STRING" id="418459.E3JRS9"/>
    <dbReference type="MEROPS" id="S14.A02"/>
    <dbReference type="GeneID" id="10528199"/>
    <dbReference type="KEGG" id="pgr:PGTG_00169"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00169"/>
    <dbReference type="eggNOG" id="KOG0840">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_058707_3_1_1"/>
    <dbReference type="InParanoid" id="E3JRS9"/>
    <dbReference type="OMA" id="RDYWMKA"/>
    <dbReference type="OrthoDB" id="2017408at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009368">
        <property type="term" value="C:endopeptidase Clp complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006515">
        <property type="term" value="P:protein quality control for misfolded or incompletely synthesized proteins"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07017">
        <property type="entry name" value="S14_ClpP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000001">
        <property type="entry name" value="ATP-dependent Clp protease proteolytic subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00444">
        <property type="entry name" value="ClpP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001907">
        <property type="entry name" value="ClpP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023562">
        <property type="entry name" value="ClpP/TepA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033135">
        <property type="entry name" value="ClpP_His_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018215">
        <property type="entry name" value="ClpP_Ser_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001368">
        <property type="entry name" value="PRK00277.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10381:SF11">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00574">
        <property type="entry name" value="CLP_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00127">
        <property type="entry name" value="CLPPROTEASEP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00382">
        <property type="entry name" value="CLP_PROTEASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00381">
        <property type="entry name" value="CLP_PROTEASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 7 9" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0720">Serine protease</keyword>
    <feature type="active site" evidence="5">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <feature type="active site" evidence="6">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP74213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="257" mass="28007" checksum="2C8585179035222E" modified="2011-01-11" version="1">MGSVRKSNLLNGVLKSAIASPGNHFNATRRPSSLLSQPRRMLSDFHRLKGSGLVPMVIEREGRGGERAFDIFSRLLRERVIFVGGEINDADANLIVAQLLYLEAESPDPIELYINSPGGSVTAGLAIYDTMQYVRSPVHTICIGQACSMGSILLGAGAKGFRKALPNSTIMIHQPSGGSSGQASDMAIRANEILRLKKRLIEIYAKHCGKEDESQTDRCNRFEQALERDKFMDGESAQAFGIIDQVVASRSSKPNDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3JS23</accession>
    <name>E3JS23_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_01487</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP74894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319313.1">
        <property type="nucleotide sequence ID" value="XM_003319265.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JS23"/>
    <dbReference type="FunCoup" id="E3JS23">
        <property type="interactions" value="38"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JS23"/>
    <dbReference type="GeneID" id="10547091"/>
    <dbReference type="KEGG" id="pgr:PGTG_01487"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01487"/>
    <dbReference type="eggNOG" id="ENOG502QU4U">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033465_3_1_1"/>
    <dbReference type="InParanoid" id="E3JS23"/>
    <dbReference type="OMA" id="MISKKWM"/>
    <dbReference type="OrthoDB" id="3358017at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007568">
        <property type="entry name" value="RTA1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465">
        <property type="entry name" value="PROTEIN RTA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31465:SF1">
        <property type="entry name" value="PROTEIN RTA1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04479">
        <property type="entry name" value="RTA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="16"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="50"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="80"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="124"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="162"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="207"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP74894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="298" mass="33806" checksum="248AFE8DAC80CA10" modified="2011-01-11" version="1">MSEQEPEMDNQHYRLFAYQPSLLLEIFFAVAFFCTMMLHTVQMIQSRSWFFLPFVLGGYFECIGFVARALLINLTYSWQLGLHITETLLLLLAPTLFAGSIYMTLTRIIRLTNGQKHSVLPVKWLTKIFVAGDLISFFLQAAGGGLDAGAKTSEEEEWGRRLIISGLIIQLVTFCLFIIHASIFYIRMKRAPTISSETLNTTWKRHLILLVGSSALIIIRSSFRLAEYIIGSHGPLMKKEIFTILLDSVLMFLCMLLFNLFHPSQALNESSDYPSEIPILETGEKGLHSSRSSLDHNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3JS88</accession>
    <name>E3JS88_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_01602</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
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                <person name="Joly D.L."/>
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                <person name="Chiu R."/>
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                <person name="Feau N."/>
                <person name="Field M."/>
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                <person name="Morin E."/>
                <person name="Murat C."/>
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                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
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                <person name="Selles B."/>
                <person name="Shapiro H."/>
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                <person name="Tuskan G.A."/>
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                <person name="Zhong S."/>
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                <person name="Grigoriev I.V."/>
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                <person name="Martin F."/>
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            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75099.2"/>
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    <evidence type="ECO:0000256" key="1">
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP75099.2"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="560" mass="64616" checksum="713EE753EB3975C3" modified="2012-04-18" version="2">MNPTHKDLEWHSEGNSDASDNSLVMAKSIPIRLSRGKPKVTEEDQDSETATQQNPKTFFLPTMNEETFIDHGCTLDAQGYPICPNGNTVFVKLPGQTITNFGNVGFSKRTGSEYRSKGTWKLKRIYCLGVLACDLPDCQWAGAPPTGRVDMREWNSHCRGARGTCPGNVAHIECKQTLIRVDEHVPTTWAILRHRGEHNHPWPEAKKPDLLSRETLKAEIAKNPKAGAFSLRIRKPNASQDTYNSVLDIHPSLGNADQLRYYRKLMLKELNITPDHLGGSVGDQFITDMFKWVKRGLLIISSSYLEGEEHFTFQSEWMRKRILARDIDHKVYGTGLVSDVTYRFFRNGYLLSTSMYCEELARWIPIQLTWIRGLAKRYYQIHFAVLFRQFVIPDFTEAERDVLVCNIVDFSAAQREGFVTAYWEVFGMCDKKVVLDKLQGCHEHYRAQVTRVKRNRHVVMADDEASSVFQTMCMDLIKQPAEGDTSHEEKIDAIRRRFPKAKRWLDWWTMADVEAMLFPSRRPKLEDAPEDQHRPTKTTNAQESLHRLYYMLSYVHDRFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3JTB4</accession>
    <name>E3JTB4_PUCGT</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
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    </protein>
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        <lineage>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75188.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003319559.2"/>
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    <dbReference type="AlphaFoldDB" id="E3JTB4"/>
    <dbReference type="GeneID" id="10542561"/>
    <dbReference type="KEGG" id="pgr:PGTG_01781"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01781"/>
    <dbReference type="HOGENOM" id="CLU_418046_0_0_1"/>
    <dbReference type="InParanoid" id="E3JTB4"/>
    <dbReference type="OrthoDB" id="2507721at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="224"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="256"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="329"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="372"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="456"/>
            <end position="487"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="542"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="577"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="614"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="542"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="622"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="632"/>
            <end position="642"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP75188.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="656" mass="74015" checksum="ACFF603F7F265192" modified="2012-04-18" version="2">MDQAKINVINYASLNKLSVGFLSIVVPLSSGLAGCLTYQFINYLNTKMFIQTQAQNQILNEVQKQRSLSTSTYHHQNNHFQEQPKKIGYTAASGDRKNHHHIGTQVVVGQLENQRADHHHHQELDLKTPHLPDPSSPFNNNYQADFFDIRADDHHNHPSDKRHWFSRLNIFQQQFEFLNDKFNVIVFFILIANLFSMALEILVFKSLAHHTLVDTGLTRVYSWPLSNLLVINYLISTGTRVYFARLASTMLRAPKLFLISSLVLLIISLCGLIFQSVLVGSHLLDYDQIKVVFTHSGSEDQTLEEFVHDLSDYFRLIGLHRIRLDLLQLAWLGPPFVWDLLISSVLMFNVLDSRKRCRFGAADGRISLGIMVMFETMFLVTLVTFMVLCICLLLGGKGGIDFERMNTLYAGFDGFVSKLHTISIFCSLQHKVNDRLRLEQKFILNSQKTRDSALSGYGLGQSVGSGNPSCPRRRSKKGPQTIHFPDFVETEIEASEAEGSDMGDDNDHDRFDGENRNVEVEEEIASIHAAENLKVTSLTFIDNRNSHPSQPHNKQNSPNLRDSNIKFPEQAKILKHHHVGRTSSTSEGLSGGPMAGAPIGLPSKLGLKAFEDLQQFLDARHPRGPRPHHSRSNPNPDLQSVVSGARPNFEPNRSVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3JTG6</accession>
    <name>E3JTG6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SUI1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_01884</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Probably involved in translation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SUI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319710.1">
        <property type="nucleotide sequence ID" value="XM_003319662.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JTG6"/>
    <dbReference type="FunCoup" id="E3JTG6">
        <property type="interactions" value="257"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JTG6"/>
    <dbReference type="GeneID" id="10542757"/>
    <dbReference type="KEGG" id="pgr:PGTG_01884"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01884"/>
    <dbReference type="eggNOG" id="KOG1770">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_082805_3_2_1"/>
    <dbReference type="InParanoid" id="E3JTG6"/>
    <dbReference type="OMA" id="VENHIHI"/>
    <dbReference type="OrthoDB" id="10248435at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016282">
        <property type="term" value="C:eukaryotic 43S preinitiation complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043024">
        <property type="term" value="F:ribosomal small subunit binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11566">
        <property type="entry name" value="eIF1_SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.780.10:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005874">
        <property type="entry name" value="SUI1_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01160">
        <property type="entry name" value="SUI1_MOF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10388">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004499">
        <property type="entry name" value="SUI1_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUI1" evidence="5">
        <location>
            <begin position="33"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP75291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="114" mass="12654" checksum="B16B7FFBDDBAF9A8" modified="2011-01-11" version="1">MSVQNLTSRDPFADVGDDQFTTTQPSTKAPDYVHIRIQQRNGRKTLTTIQGLPSEYDPKKLLKAFKKDFACNGTLVPDEELGQVIQLQGDQRTKVSLLLVDAGIPKATIKIHGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUU0</accession>
    <name>E3JUU0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01146</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75815.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320234.2">
        <property type="nucleotide sequence ID" value="XM_003320186.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JUU0"/>
    <dbReference type="GeneID" id="10543809"/>
    <dbReference type="KEGG" id="pgr:PGTG_01146"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01146"/>
    <dbReference type="HOGENOM" id="CLU_2050784_0_0_1"/>
    <dbReference type="InParanoid" id="E3JUU0"/>
    <dbReference type="OrthoDB" id="10268175at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171553" evidence="1">
        <location>
            <begin position="20"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75815.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="120" mass="13214" checksum="6D5A7753DC0882AC" modified="2012-04-18" version="2">MHFYTFVIALWATCGFAAAFDKTKQFQCPPTGKSYGFCGFPVATHPENKLTYWSLAQITPPDNNTPNIFNRCGWPSTANLCGDSAMVAYVFSKLGSSDGIPEDDNWSPHIFLESHVPSQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUU9</accession>
    <name>E3JUU9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_01155</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75824.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320243.2">
        <property type="nucleotide sequence ID" value="XM_003320195.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JUU9"/>
    <dbReference type="GeneID" id="10543818"/>
    <dbReference type="KEGG" id="pgr:PGTG_01155"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01155"/>
    <dbReference type="HOGENOM" id="CLU_019847_0_0_1"/>
    <dbReference type="InParanoid" id="E3JUU9"/>
    <dbReference type="OrthoDB" id="2510720at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="129"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="390"/>
            <end position="486"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="505"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="188"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="138"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="434"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="447"/>
            <end position="486"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP75824.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="530" mass="57836" checksum="2148E359D97DFDBD" modified="2012-04-18" version="2">MTNHTAPNAHTGSCGYQTNLTTSPTDLSDGIGQKHHTHPAPRYQVNTSMDSHLHTSGNLLTANGLLLHSYRPLTNSTYGSLGHDDQMHNHPLEGRATNIEVIGGGYGTPSEASGAVSELIVGAQTMASSGLSDPRTVGQATPPSGNLTPAAPGRINPFLASAGAKTRLKSSSTRKQNRTRAEIDADKAVTAAKKAERIQKAAQKQEAQRQRLAAKEARAALKVSDAGTPTPRFVWSKEASLELLRYVKEIKEEHDNLIKRTPGFIAWTPYFLKCEADRGDYPLLIGIANDVILRRYQALMNMWKVVFDKLSHSGSGGLYEVLDKEHLSETIWDFINAMHGDNAAANAHGHAELDDDLGALLCEDGRGEGREARTGAEAELSQEATAELALDNSDNEDLDNPNQTTMGGGPVPEFSLFDGIPRAPACPLTSTQPDPTPIQAPPAPETPAREAARVEDRQMAAKKAEDKEAARKAELTQAAHDRSMQEVQLEIDRELAKKRNEVLDEERRVRDEDRREEKRLAHEWRAEEAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3JVK8</accession>
    <name>E3JVK8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_02524</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76083.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320502.2">
        <property type="nucleotide sequence ID" value="XM_003320454.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JVK8"/>
    <dbReference type="GeneID" id="10533487"/>
    <dbReference type="KEGG" id="pgr:PGTG_02524"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02524"/>
    <dbReference type="eggNOG" id="ENOG502QQR9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048932_2_0_1"/>
    <dbReference type="InParanoid" id="E3JVK8"/>
    <dbReference type="OrthoDB" id="78198at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48471">
        <property type="entry name" value="DDE TNP4 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48471:SF1">
        <property type="entry name" value="DDE TNP4 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="3">
        <location>
            <begin position="70"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP76083.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="285" mass="32762" checksum="C60C4911B5D926E4" modified="2012-04-18" version="2">MASYTLQQIFAITPSVCSRYLGGGLQVLLRVLQTLPNARITWPSSEERCQSYSDMITRKYPLLKKCFAFIDGLNLPILVSGDDDVQNAYYNGWTCSHYCSSILTFAPDGTLIYAVLNTPGPWYDSYIAEPLYQKLLNNTPLGYRVISDTAFPRKGARLQGRILAPTKRGEKMPSSDSEFAHKKVLNEQLVCARQAAEWGMHSIQGSFARIKLPLPADDHQFRTDVLQVICRLHQIRCREVQINQTRSVYMSTQDEQKMLCQKFHWMLFSHIQQNCRISRYYQGWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWD5</accession>
    <name>E3JWD5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Oxidoreductase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_02801</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003320731.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JWD5"/>
    <dbReference type="STRING" id="418459.E3JWD5"/>
    <dbReference type="GeneID" id="10534253"/>
    <dbReference type="KEGG" id="pgr:PGTG_02801"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02801"/>
    <dbReference type="eggNOG" id="ENOG502SCAE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1644574_0_0_1"/>
    <dbReference type="InParanoid" id="E3JWD5"/>
    <dbReference type="OMA" id="NIQETSM"/>
    <dbReference type="OrthoDB" id="10064411at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019180">
        <property type="entry name" value="Oxidoreductase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039251">
        <property type="entry name" value="OXLD1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21193">
        <property type="entry name" value="OXIDOREDUCTASE-LIKE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21193:SF3">
        <property type="entry name" value="OXIDOREDUCTASE-LIKE DOMAIN-CONTAINING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09791">
        <property type="entry name" value="Oxidored-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Oxidoreductase-like" evidence="2">
        <location>
            <begin position="101"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="139"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP76360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="183" mass="20347" checksum="21987B0288BD13AF" modified="2011-01-11" version="1">MTPAGASLCTTKKPRSGRKNSPRPPNGDAPGLEKSDSPLKPHVGSTWRPIDSSRLNLRHQFTSNQIGSSPPTSLAQDVLHIRSNQIDKRKDIQSSPEIIGPPKPPGPEDCCMSGCATCVYDIYAQETEEYVKSLKQRNDLNQSQDTTSKSQETTMNLTEEIDDQVILNNSLKVFARFEKTRHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXC2</accession>
    <name>E3JXC2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Velvet domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02158</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003321068.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXC2"/>
    <dbReference type="GeneID" id="10528636"/>
    <dbReference type="KEGG" id="pgr:PGTG_02158"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02158"/>
    <dbReference type="HOGENOM" id="CLU_1563617_0_0_1"/>
    <dbReference type="InParanoid" id="E3JXC2"/>
    <dbReference type="OrthoDB" id="2498404at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.3960">
        <property type="entry name" value="Velvet domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037525">
        <property type="entry name" value="Velvet_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038491">
        <property type="entry name" value="Velvet_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11754">
        <property type="entry name" value="Velvet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51821">
        <property type="entry name" value="VELVET"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Velvet" evidence="1">
        <location>
            <begin position="1"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="188" mass="21540" checksum="D9397DFA441EA39B" modified="2011-01-11" version="1">MRFWRQAAPGAGEDDQALVPIDLEEELVRHPLAPAPVLEALRYENGRQVIMSADDATRLIVSAHPILLNDDGNPIEEVPLDAINFDPPRGALYDPPNAPAEDASWIFVFDDIGIRTLGRYVIHFCLMRIPRECEEPPEQLAHATTDAFEVVEEARWPLFTDFYTELSARLNRDHDHLNIYRPRYQDLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXI4</accession>
    <name>E3JXI4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyanovirin-N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02220</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321178.1">
        <property type="nucleotide sequence ID" value="XM_003321130.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXI4"/>
    <dbReference type="GeneID" id="10528780"/>
    <dbReference type="KEGG" id="pgr:PGTG_02220"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02220"/>
    <dbReference type="HOGENOM" id="CLU_119658_0_0_1"/>
    <dbReference type="InParanoid" id="E3JXI4"/>
    <dbReference type="OrthoDB" id="3361140at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173052" description="Cyanovirin-N domain-containing protein" evidence="1">
        <location>
            <begin position="28"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="19936" checksum="5A00793330614066" modified="2011-01-11" version="1">MFLKTPSIVLLATGIVTFNLLSTGSMGESVCANWWKSLPAGQKPPVNVALAPQINKKESGAKLPGRINGNLAGRNTTFLSPGDGACGITYTNEDKGACLWGGLNSVEPIPQGQQSGWLTGTNTKNCGKKLGKHRATGKFLDSCSLEEDKQKLTLDQGCSTIYVTRAIFIKLGGDPGGIGRIQIDNWDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXN3</accession>
    <name>E3JXN3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein mS29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_02269</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the mitochondrion-specific ribosomal protein mS29 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76808.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321227.2">
        <property type="nucleotide sequence ID" value="XM_003321179.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXN3"/>
    <dbReference type="STRING" id="418459.E3JXN3"/>
    <dbReference type="GeneID" id="10528910"/>
    <dbReference type="KEGG" id="pgr:PGTG_02269"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02269"/>
    <dbReference type="eggNOG" id="KOG3928">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042567_1_0_1"/>
    <dbReference type="InParanoid" id="E3JXN3"/>
    <dbReference type="OrthoDB" id="274828at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019368">
        <property type="entry name" value="Ribosomal_mS29"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12810">
        <property type="entry name" value="MITOCHONDRIAL 28S RIBOSOMAL PROTEIN S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12810:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN MS29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10236">
        <property type="entry name" value="DAP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="19"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP76808.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="457" mass="50984" checksum="DE1DED2A5C776223" modified="2012-04-18" version="2">MTSKTRTLITQLILNQQRRTNNTLLNTPPPSLRFKSSGSSGGGSRTPIKIKINKKDADPGPRPGPDPGQLVNLDYITQPPINLSAVPDLEPSKMTSENVGKSFKFSKQMAKAIDTFSIPSSISKDWKAMSVPVTTLRKASVDLISRLAQAKKSTTTDKPNVLTLNGQAGTGKSVTLLHGLSYAVQNRWIVIYIPDAKCVVDGQYSYEYCPRTETYHQNSLSAELIRKIIAINDLEGLTLTKGCKLFGTKDDRMVDYEEEIPAGTGLKNLAQLGIQMTHLAPNVLQILFEELAAQSQKPVFFAIDGAQNLFKPSDYVDGSFRKIDSFAFNVARLFLNFARGTQKWSKGLTILSSSSQHCPTKSLAWDRLIKDRQDSIVDREAWPGWEHYQKLIKPIQAYPNLDVQNLDRVEALGVAQSFELSRLVLAPLDDRVFLRHFICSNGNVREFTREIKNQIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXV5</accession>
    <name>E3JXV5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Glucosamine 6-phosphate N-acetyltransferase</fullName>
            <ecNumber evidence="3">2.3.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_02341</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>D-glucosamine 6-phosphate + acetyl-CoA = N-acetyl-D-glucosamine 6-phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10292"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57513"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="EC" id="2.3.1.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I): step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the acetyltransferase family. GNA1 subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.4" evidence="3"/>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321299.1">
        <property type="nucleotide sequence ID" value="XM_003321251.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXV5"/>
    <dbReference type="FunCoup" id="E3JXV5">
        <property type="interactions" value="153"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JXV5"/>
    <dbReference type="GeneID" id="10529065"/>
    <dbReference type="KEGG" id="pgr:PGTG_02341"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02341"/>
    <dbReference type="eggNOG" id="KOG3396">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072095_0_1_1"/>
    <dbReference type="InParanoid" id="E3JXV5"/>
    <dbReference type="OMA" id="NQRYDWI"/>
    <dbReference type="OrthoDB" id="10039976at2759"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00529"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004343">
        <property type="term" value="F:glucosamine 6-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000043">
        <property type="entry name" value="Glucosamine 6-phosphate N-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039143">
        <property type="entry name" value="GNPNAT1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13355">
        <property type="entry name" value="GLUCOSAMINE 6-PHOSPHATE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13355:SF11">
        <property type="entry name" value="GLUCOSAMINE 6-PHOSPHATE N-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="4">
        <location>
            <begin position="28"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU365086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP76880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="189" mass="21485" checksum="3C89AA83DBFB9F93" modified="2011-01-11" version="1">MTFTANSDLDLLFDPKWLAENPELGSDLHIRPLSKTDYERDHLKLLAGLTSAPDTGLTDYQARFELLRDVNKATPGRPSYCIICIVRKSDDRLVASGTLLLEHKFLRACGSVGHIEDIVVDPDVRGKSLGKQIIKSLTETSEKLGAYKTILDCNKDNIPFYEKCGFQHKEYEMVRYTPEEIVARYKKNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>E3JYG2</accession>
    <name>E3JYG2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Adenosinetriphosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_03043</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion outer membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77087.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321506.2">
        <property type="nucleotide sequence ID" value="XM_003321458.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JYG2"/>
    <dbReference type="FunCoup" id="E3JYG2">
        <property type="interactions" value="303"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JYG2"/>
    <dbReference type="GeneID" id="10529435"/>
    <dbReference type="KEGG" id="pgr:PGTG_03043"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03043"/>
    <dbReference type="eggNOG" id="KOG0737">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_21_14_1"/>
    <dbReference type="InParanoid" id="E3JYG2"/>
    <dbReference type="OrthoDB" id="10254455at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005741">
        <property type="term" value="C:mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140567">
        <property type="term" value="F:membrane protein dislocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140570">
        <property type="term" value="P:extraction of mislocalized protein from mitochondrial outer membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034214">
        <property type="term" value="P:protein hexamerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006626">
        <property type="term" value="P:protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000538">
        <property type="entry name" value="ATPase family AAA domain-containing protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051701">
        <property type="entry name" value="Mito_OM_Translocase_MSP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45644">
        <property type="entry name" value="AAA ATPASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G12920)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45644:SF3">
        <property type="entry name" value="FI08533P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3" id="KW-1000">Mitochondrion outer membrane</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="7">
        <location>
            <begin position="171"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP77087.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="408" mass="45364" checksum="372B815F750FB602" modified="2012-04-18" version="2">MARDRPGDPSGATVTLSTPLGTWHDYVHSSRPQHQQHPKKMTFLNQLKLSNFSPTTKKAFSDLTLLAISQIFLFYGVKYILARADPSRQSKDHLLKKSKKVMKSMGLDKKTLELDDHEAMLIGEVIQPDEIDVGFSDVGGLDPIINDLKESIIFPLCYPSTFKSSAGLFSSPKGVLLYGPPGCGKTMLAKTLAKESGAMFINIKPSDLSSKWFGESSKLVAALFSLSQKLQPSIIFIDEIDSFMRERSRTDHEVSGMMKAEFMTLWDGLATGSSRILVLGATNRPNDIDPAILRRMPKRIPVGLPNLQQRTNILNLLLKDIKMEANLSIEYLAQQTTNYSGSDLKEFCRSAVMAPIKEYVRSKGGDKSAMVEASQSELELRPIRLDDFEIEKNLKHEPNSVVPIASLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZ15</accession>
    <name>E3JZ15_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">NADH:flavin oxidoreductase/NADH oxidase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_03246</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NADH:flavin oxidoreductase/NADH oxidase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77290.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321709.2">
        <property type="nucleotide sequence ID" value="XM_003321661.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZ15"/>
    <dbReference type="FunCoup" id="E3JZ15">
        <property type="interactions" value="1"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JZ15"/>
    <dbReference type="GeneID" id="10537835"/>
    <dbReference type="KEGG" id="pgr:PGTG_03246"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03246"/>
    <dbReference type="eggNOG" id="KOG0134">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012153_6_3_1"/>
    <dbReference type="InParanoid" id="E3JZ15"/>
    <dbReference type="OrthoDB" id="1663137at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04733">
        <property type="entry name" value="OYE_like_2_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051799">
        <property type="entry name" value="NADH_flavin_oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001155">
        <property type="entry name" value="OxRdtase_FMN_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43656">
        <property type="entry name" value="BINDING OXIDOREDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G08260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43656:SF5">
        <property type="entry name" value="NADH:FLAVIN OXIDOREDUCTASE_NADH OXIDASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00724">
        <property type="entry name" value="Oxidored_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADH:flavin oxidoreductase/NADH oxidase N-terminal" evidence="5">
        <location>
            <begin position="23"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00724"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP77290.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="407" mass="44830" checksum="A3DF29A29193A23D" modified="2012-04-18" version="2">MPLMDPIGILSQPLRFQGGLTAPNRTMKSALTERLCSYDQLDLDARGKPTQEYEELYKVWSQGKIGIIVLGNIPIHREYLEAMGNAIIDKDSPWDPVEAFKPAIATAKTHGSLVIGQLTHAGRQTPLDVNPNPVSASESQSPPCFGMSFGKARALSLDEVHDVVERFAYGAEVLYNAGADGVQLHGAHGYLLSQFLSPRVNKRTDQYGGSFENRSRIVFEIIEAIKKRVADRKFILSIKYNSQDFIDGGFTKEDSQMMAVRLEAMGVELLELSGGTYELMPFEEKKASTLRREGFFLEFADGIRPHLTGASVLAVTGGFRTLEGMASAVSSKQRTCDLVGLGRPLVFEPHFVADLLAGKTKQAKLLAFDSMFLAVAYHVLKLIAHQQPIPDLEDENIAKEIIELVST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZ58</accession>
    <name>E3JZ58_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Clathrin/coatomer adaptor adaptin-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_03289</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the adaptor complexes large subunit family.</text>
    </comment>
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        <property type="protein sequence ID" value="EFP77333.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003321704.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZ58"/>
    <dbReference type="FunCoup" id="E3JZ58">
        <property type="interactions" value="298"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JZ58"/>
    <dbReference type="GeneID" id="10538039"/>
    <dbReference type="KEGG" id="pgr:PGTG_03289"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03289"/>
    <dbReference type="eggNOG" id="KOG1060">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006320_3_2_1"/>
    <dbReference type="InParanoid" id="E3JZ58"/>
    <dbReference type="OrthoDB" id="10254310at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030117">
        <property type="term" value="C:membrane coat"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026739">
        <property type="entry name" value="AP_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002553">
        <property type="entry name" value="Clathrin/coatomer_adapt-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11134">
        <property type="entry name" value="ADAPTOR COMPLEX SUBUNIT BETA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01602">
        <property type="entry name" value="Adaptin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Clathrin/coatomer adaptor adaptin-like N-terminal" evidence="7">
        <location>
            <begin position="135"/>
            <end position="656"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="791"/>
            <end position="921"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="800"/>
            <end position="825"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="826"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="877"/>
            <end position="911"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="912"/>
            <end position="921"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP77333.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="921" mass="104016" checksum="0258FDC98E2F2BE1" modified="2012-04-18" version="2">MDLSTLANHAQRLSARLSENLAERTKDLGLDPEYNLSNPSNTLNNKLPILAHAQGLLGNSLPYWERLSELTSVEGELETKRLLGSKSESDRVEGLKRVTVIMCRNRPVLSFFPFVTNCLHPSTSSMANGSYHSTVSHSFTIRHLVSIYILQHSHLSPDLALLSVNAWQKDLSDPSPIIRSLALKTLAGMGLESVLPLVVVTIDRLANDPNWFVKRTVAEALITVHQMDPHTYRDKLIEGPVKRLLQDRSPLVLGAALTAWEAICPEKWDLLHRRFRAFCWTLVDADENGQHVIMRTLTRYVREHFTNPEQPTDSKTPLDLDLELFLNSVHTLFQSRSSAVVIAATNVFIFLAPLDRLKTVVPDLMRLVEEKASEREDESLLVVLNQIYQIIKFTSNKLNKSHLFSAHLNSFGICSTSDSQAVKISKLKILRTFISLNDRPIQLRVLSELKFCTRDFHDEFVKQVIRTIGRIVMMTNGKVQQEALDVLIELTKTGQNKQRCKSVEILRGILSSDLKTIAAMLPSLQKVKGDLISLLVHNKIQQDSARANLYRLVSDIDFRSEAPISPSFIHFYKYSIWNFANEGLRCKFQILSLVSKSLLMITIRRQEDDASMSSVLFDQRRAYQLGFDHLMRVARYDSNFIVRDRARFLQGLLTSTQGLTLHDEPKGFGLVEVHEHDEHHEGAIALLDEHTAFTRGHQLSSLHDPSSCSIDQEPMKKISLNELKKILLLDPSPEPVNLDDYQGRSYFEANSLFRMAGLNPGERGDGNSSSWRYLDWNTVPAWSQTALDPRLREPEDGQEKTQNSSAQLSSAFHQDPSNPSRTSTTDGKRMIDRSKLKEKVVLIPTGSDDDLISTNTRPSGTGGSGHRNLEDFLQSSGDEDDGLQDEDEDESEDESEDEDEDEDEDESESENDDTHNHINGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZS4</accession>
    <name>E3JZS4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03505</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321968.1">
        <property type="nucleotide sequence ID" value="XM_003321920.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZS4"/>
    <dbReference type="GeneID" id="10538588"/>
    <dbReference type="KEGG" id="pgr:PGTG_03505"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03505"/>
    <dbReference type="HOGENOM" id="CLU_1696382_0_0_1"/>
    <dbReference type="InParanoid" id="E3JZS4"/>
    <dbReference type="OrthoDB" id="2501270at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="155" mass="17417" checksum="3E10060E57AA24F1" modified="2011-01-11" version="1">MGELRRTNIDWDCSEPRVPKTSDRSKIHSNRHREDMEICEELRYGCAGGYWSDLSESGKTGSETPVARYSAVDSNEGCTTPPAREGMGKTSGLSKLRQTEIYAGLRTNSKWKELHKAQIQKSFKKRVKMFHRISERDLMSSASCDTPAQPSADTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3K0M6</accession>
    <name>E3K0M6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03807</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003322222.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K0M6"/>
    <dbReference type="GeneID" id="10540835"/>
    <dbReference type="KEGG" id="pgr:PGTG_03807"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03807"/>
    <dbReference type="HOGENOM" id="CLU_032240_0_0_1"/>
    <dbReference type="InParanoid" id="E3K0M6"/>
    <dbReference type="OrthoDB" id="2506702at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="511" mass="56439" checksum="7D4D7C1D0EFD7886" modified="2011-01-11" version="1">MSDSSSPLLSTRMRLPSPTPTPPTHFLSPRPNYLSPSPSLPPLSPQPNDFDPRPIPPMSPTLLAAALPYLPVPDLPTVIPATARDDGVSSLISHLGIENQSLNDTILSHAQKIADLEIQQRRYLKLFDDLKSLLPLSSQFMKVKTDSSDQFLQVRNAIDSVNNSTASRLDSVEGFVSQSITHPPIQPKSFRDPPYLSHIYFSGVLSETKEFCFAMRHSLEMHGDNLHSEKHKVMWIAGYFRKPDGKLGDYCASYVWWRGLMAKNAHHQNLDPNTASSKAAFLLEELLSGEAFLVAIETTFSNHKGEEEARKSLRVMKQGNTSIEEFNVSFNSLLYAVDLSEASKCDLYDEAINPKIVQLGLLRGGWNGVTDLAKKQAMAVELAADVPGVAMIDRLSQKPQPIVQKIQLPPKPDSSKAPPRLLDGVPMDIDAIAAELGSTYDAYRHECVLQHICHRCGGRYDSAHSDARRSPVERNSQLSKPQILSIWRDWGGSDGIEGQSSRGSQSISAID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3K1A8</accession>
    <name>E3K1A8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP78083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003322454.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K1A8"/>
    <dbReference type="STRING" id="418459.E3K1A8"/>
    <dbReference type="GeneID" id="10541553"/>
    <dbReference type="KEGG" id="pgr:PGTG_04039"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04039"/>
    <dbReference type="HOGENOM" id="CLU_052859_0_0_1"/>
    <dbReference type="InParanoid" id="E3K1A8"/>
    <dbReference type="OMA" id="ISANEYQ"/>
    <dbReference type="OrthoDB" id="2552152at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="63"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="376"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="417"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="427" mass="46936" checksum="61C8458B6BC779F3" modified="2011-01-11" version="1">MKRGRKQDDTLPPSRSREIQRAFRQRRSDYIKGLEARVHELEIEVDEVLARHNELLRYTTPEVVAARSASRKKQRNKSKHSLSQFLDVNQPVEVYAHSPSDPLAPLPENSMVPPSSVGSNQSMGSDISPATPAQYIFTDMNGDPPSAQNFSKGPFAPFSFAGQVEHQHQYVCRDNDSSSILARRNQRLLHLWDGVPKANDLSGVSAESPGPPSSQFSHPISASEYRCSTSQIEMLMGIEPVGQSPVVSSQLMSSFDLHHEVRVPPPSSCSLPSLVADSSVQPDQLSVIGFNTYTSNSDPDASPYAFRSPHSQISDRMNPCYTFMDSSNSSSPNASVYRHESPLDVFHPRPLSSQHNQPNGTLSDLISLATTKLDHFEPPKECSYPTSNLQPSPPLAESPVSPDSLENSELSNDDRSSISPGENPTFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3K1Z1</accession>
    <name>E3K1Z1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04316</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003322731.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K1Z1"/>
    <dbReference type="GeneID" id="10542314"/>
    <dbReference type="KEGG" id="pgr:PGTG_04316"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04316"/>
    <dbReference type="HOGENOM" id="CLU_325748_0_0_1"/>
    <dbReference type="InParanoid" id="E3K1Z1"/>
    <dbReference type="OrthoDB" id="2500831at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="424"/>
            <end position="446"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="766"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="811"/>
            <end position="853"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="424"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="766"/>
            <end position="779"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="811"/>
            <end position="821"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="838"/>
            <end position="847"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="885" mass="101561" checksum="F70DD2D0C348836C" modified="2011-01-11" version="1">MNDRSLRPVIQLLGSPSHLIFQLVRFTTLPYMIYQIINNQKQKQKQLAEQASDEHEPEPPRIIIKTHKPVKHNTPNQPRRSFSTETQDDYSTLFPQLIKEQPRLNQQLIHLLDHFLNLNHTPQLSQQTVEQLFLISRSTIRDRLNPHSTEIRTPTSTAWQAEAMAQIVQHWSNQRHHHPTKPIDQLNPSNSISKSLKIHSRTRAFLSNGPPRCDCQRSNLPLRTIELGLDQVGTLQSSSTLGLFASKLNHSLSPLTILRIIDLARQAQRWDLAVIFLDRSLAEPSILNDPKLFNRRSGQVTALLDFLRHHGRSEPILDPLRLWCIHFICQSIETTINHHPQHQWTRINRMSLDVLTRFASMPIGFDRRLKQDQLNRQQPIYPCLDLNRHQILLDAITHSTNELEFAVLTRERCERLLSKLTRLTTRHSPPLDPSSSSAATSSNSPQVHAWATRSDIQLRLLEYLARQGTQEQFMSLLQTHVEQHPPGPKLARIILLGARRFDSLSANPELIRHTLSQLDQADQRPIIDFILRPRQSLDTLRANLEICLASLPVEREPRMRIYSHLLFRCSQLASPSLALKLWETINRDERRRSLIRVGRRTRAKLKDLLRNQKIQPLALTSSTDLLSHQVIRSMIYVFKNLTHRSSSSSSSANQLAIGKHYRAMYQRLSLKHSHKAKWPLSRSQARSHLVLHLLKTELGILKGKEMRHDGNGLVRALESCFVDPCVGDLNGFEAVLKFLLLSKSTNKTLESVLLRRLVYLDSLELPPSHRSSSSSLSNRPETRDRARRQIDRLAIRRRFYRFVVLWRRSRGGSRPAHRPHPHPAPSTPCLSLHHHPSIPSTSVPHSTPTKKNRRACSRFWASFVALHSTPPPPPSSSSSSSPSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2C2</accession>
    <name>E3K2C2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Dynamin-type G domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_04447</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78491.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322910.2">
        <property type="nucleotide sequence ID" value="XM_003322862.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2C2"/>
    <dbReference type="STRING" id="418459.E3K2C2"/>
    <dbReference type="GeneID" id="10536172"/>
    <dbReference type="KEGG" id="pgr:PGTG_04447"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04447"/>
    <dbReference type="eggNOG" id="KOG0446">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_376041_0_0_1"/>
    <dbReference type="InParanoid" id="E3K2C2"/>
    <dbReference type="OrthoDB" id="2583551at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016559">
        <property type="term" value="P:peroxisome fission"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd08771">
        <property type="entry name" value="DLP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1240:FF:000043">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:004690">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1240">
        <property type="entry name" value="Dynamin, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022812">
        <property type="entry name" value="Dynamin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001401">
        <property type="entry name" value="Dynamin_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045063">
        <property type="entry name" value="Dynamin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000375">
        <property type="entry name" value="Dynamin_stalk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030381">
        <property type="entry name" value="G_DYNAMIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566">
        <property type="entry name" value="DYNAMIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11566:SF21">
        <property type="entry name" value="DYNAMIN RELATED PROTEIN 1, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01031">
        <property type="entry name" value="Dynamin_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00350">
        <property type="entry name" value="Dynamin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00195">
        <property type="entry name" value="DYNAMIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00053">
        <property type="entry name" value="DYNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51718">
        <property type="entry name" value="G_DYNAMIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dynamin-type G" evidence="3">
        <location>
            <begin position="32"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP78491.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="594" mass="67566" checksum="1BE0BF905A381477" modified="2012-04-18" version="2">MSSSVEDATAISCPRTLLDKVDEVRKLGLADKIPLPQIAVVGDQSSGKSTLLEYISGVTFPKDSGMCTCFVTEVMMRPAEEFSARVLVNGEVDSRLKVPESKDDVAAVIENAKALFMDGEKRVIYDDILTVELSGPELPMLTLVDLPGYVQTHTLGQSETIVQEIENLVEKYISEPRTIILAVIPATRDFETNVAIKYIRQFDEQGKRTLCVLTKPDLVDRGTESRVFETLAGDKMHLSRGYHIIKNKSYEDCRAGDPREETLKKESNFFGRAPWSSIPVTDRGIQNLIEKLTDTLVDQVQKEFSGIKKDVIQRKEKLLQQLKALGPVIETDLEKANLLQKNINEVMQQFKYLVDGHYGAGGFGQDLYLRSLVRDLNEVFNARIIRMTNSTTSHLDVREIMKATRGRELRGMVPLEAFIILCRRVVQDWSSETHQHITEVCQLASNVFAQVIEKRCDKVLVNYFSERMIEFVDQQQKAMHHDALEILDDEINLPSTLQDTDFAKKWGTDENPEDNQMREILASYCLTAASRYIDAICMYVIERGLFKNCDVRGIKWFMDDPSALSRFREPRQNGRLREILPKEIQKLQDAISRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2Q6</accession>
    <name>E3K2Q6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_04581</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78625.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323044.2">
        <property type="nucleotide sequence ID" value="XM_003322996.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2Q6"/>
    <dbReference type="FunCoup" id="E3K2Q6">
        <property type="interactions" value="894"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K2Q6"/>
    <dbReference type="GeneID" id="10536478"/>
    <dbReference type="KEGG" id="pgr:PGTG_04581"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04581"/>
    <dbReference type="eggNOG" id="KOG0270">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023867_0_1_1"/>
    <dbReference type="InParanoid" id="E3K2Q6"/>
    <dbReference type="OrthoDB" id="270624at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:002899">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044285">
        <property type="entry name" value="PWP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14091">
        <property type="entry name" value="PERIODIC TRYPTOPHAN PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14091:SF0">
        <property type="entry name" value="PERIODIC TRYPTOPHAN PROTEIN 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="313"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="364"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="461"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="50"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="267"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="50"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="86"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="98"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="267"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="292"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="303"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP78625.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="592" mass="65775" checksum="DC9C960877A92356" modified="2012-04-18" version="2">MASLISSTVWVKRGQPAQHPKRYNLDKNELERVSRLAGDRLAQVKELLDEQVEAEEEELVDVEEMIAGEEEDDEEEDNDEKKQENSDNEWSDQDSSSEPDKMDLDDPSKPTDEMAQYNLDDYDKEESKGVSMGAFSNIKGLQFYQNQTDDPYVTLDDLANDETHEREELEIYPTDNLIIAARTEDDVSQLDIYVYDQGEENLYVHHDLLLPAMPLCLEWIDFSPAGIDCDDPTRKGSFIAVGTMDPEIEIWNLDVVDGLYPDAILGNNNNPSSQVDQPSAEQVSDSNIQSKKDKKKKKKNNKPQPSTLNLSPATHHTSSVLSLSHNKLARNLLLSASADTTIKLWDLNQAPSGPSSTFSAIESFQMHTDKVQSAQWNPKEATVVLSGGWDGMLKVWDTRNSGEGVEVKVDSDVECLRWDPFNPQAFIVTLDNGLIQSFDSRMLSQFSTTPAKKTAKPLWTLSAHDSSVSAFDISPVIPGLLVSGGVDKMVKVWNLEDKSGSPKLSMVVSRDLGVGKVFSVGFCPDDPTTIAVAGSKASLQIWDLATNNGVRSVFRDRLKQFSSSLDFENRKPIGKDGVLSVVDDLESDDDDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3K354</accession>
    <name>E3K354_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04867</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78911.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323330.2">
        <property type="nucleotide sequence ID" value="XM_003323282.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K354"/>
    <dbReference type="GeneID" id="10537099"/>
    <dbReference type="KEGG" id="pgr:PGTG_04867"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04867"/>
    <dbReference type="HOGENOM" id="CLU_095050_1_1_1"/>
    <dbReference type="InParanoid" id="E3K354"/>
    <dbReference type="OrthoDB" id="2498688at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171727" evidence="1">
        <location>
            <begin position="24"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78911.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="218" mass="24172" checksum="13CE96566745E994" modified="2012-04-18" version="2">MMLSYRFVLIAIISLLLTSTVNARCYRRPANQNHCNGAINKITFQEGKLPMIENEVVAILDTCSLIVRNLHQQVVTKDLIKSGVAELFKQCPGKCGSVKVPSNPKVSVLVKMRAPAGTQWEAWNADAELNKPYCYQPNGEPEVKSKYDCIKAYNQIPIDSQGHLTHKSKGLLTQMGVTFGSCTLALTSTDGSIFQLNKKDTAPIITKMIQQCDKKVRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3C8</accession>
    <name>E3K3C8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04941</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78985.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323404.2">
        <property type="nucleotide sequence ID" value="XM_003323356.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3C8"/>
    <dbReference type="GeneID" id="10537173"/>
    <dbReference type="KEGG" id="pgr:PGTG_04941"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04941"/>
    <dbReference type="HOGENOM" id="CLU_1046390_0_0_1"/>
    <dbReference type="InParanoid" id="E3K3C8"/>
    <dbReference type="OrthoDB" id="10312637at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78985.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="266" mass="29877" checksum="5D81746A53E55E94" modified="2012-04-18" version="2">MPLPTDSQSHQEAAVAQPSSGGDPASSYTSMGRATDGLSSADVDPEPIIVEAQVLTHQPSTARFSQFHRNRFLNSGRISDPALKENHRKFRNKLNEKRFHCSRHPRNCVDPSKLRVHYELPFAMVEPAFPDPTAPDGTKILRILTGPTGHLQKTAGMNALYKQLLAALNEHHENALKRSGRRSASSQSAWLKWLWTEMFKPQKSLPLCGIVHAPYPLWTLGEIQVELIKYFAQNPQDNKLLLSTASHLLRAYFKAHDGSQQMLHYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3D4</accession>
    <name>E3K3D4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AB hydrolase-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04947</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78991.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323410.2">
        <property type="nucleotide sequence ID" value="XM_003323362.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3D4"/>
    <dbReference type="STRING" id="418459.E3K3D4"/>
    <dbReference type="GeneID" id="10530812"/>
    <dbReference type="KEGG" id="pgr:PGTG_04947"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04947"/>
    <dbReference type="eggNOG" id="ENOG502S15T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046066_1_3_1"/>
    <dbReference type="InParanoid" id="E3K3D4"/>
    <dbReference type="OrthoDB" id="1263307at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052897">
        <property type="entry name" value="Sec-Metab_Biosynth_Hydrolase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37017">
        <property type="entry name" value="AB HYDROLASE-1 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37017:SF11">
        <property type="entry name" value="ESTERASE_LIPASE_THIOESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="1">
        <location>
            <begin position="10"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78991.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="262" mass="28770" checksum="A5699214260EE0E5" modified="2012-04-18" version="2">MSDTKRPVLFLIAGAWHGSWMFDSVNMTLTEAGYTTSLIELPSTGEPDGLPQGERDDAEYIRSRILEEIDKGGDVAIVCHSYAGVPTCDAVKGLGIGERQQNQQAGGVCSLIFVAAFVLWSGTSVNELNDKHQHAEFHYQEGLVWPARPENLFYNDLPNGFAVELSRLLRSHSAGVFASPLDHEAYRFIPSSYLICTEDNAIPLAAQEEMLADKLGYFLLVERLASGHLPFISRPQETVDFITKTLSLVLTKPPPSQLGTSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3L6</accession>
    <name>E3K3L6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05029</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP79073.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323492.2">
        <property type="nucleotide sequence ID" value="XM_003323444.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3L6"/>
    <dbReference type="GeneID" id="10530894"/>
    <dbReference type="KEGG" id="pgr:PGTG_05029"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05029"/>
    <dbReference type="HOGENOM" id="CLU_038374_3_1_1"/>
    <dbReference type="InParanoid" id="E3K3L6"/>
    <dbReference type="OrthoDB" id="5392716at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058913">
        <property type="entry name" value="Integrase_dom_put"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177:SF1">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24764">
        <property type="entry name" value="rva_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase core" evidence="1">
        <location>
            <begin position="42"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF24764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79073.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="120" mass="14009" checksum="595DDB72081690B2" modified="2012-04-18" version="2">MRMILMREYDIQIPQRLVYDVLREIDPEGMAARLRGVCKRRIYRTNGPNHIWSSDGHDKLKCFGLTIYGFVNAWSRKILGMFVHVTNNNPRHIAVYFLQLASKAGGVPLLLTTRLWYQNC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3K5B5</accession>
    <name>E3K5B5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_06032</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324130.1">
        <property type="nucleotide sequence ID" value="XM_003324082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K5B5"/>
    <dbReference type="STRING" id="418459.E3K5B5"/>
    <dbReference type="GeneID" id="10533072"/>
    <dbReference type="KEGG" id="pgr:PGTG_06032"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06032"/>
    <dbReference type="eggNOG" id="KOG0379">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_385928_0_0_1"/>
    <dbReference type="InParanoid" id="E3K5B5"/>
    <dbReference type="OrthoDB" id="2496273at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051285">
        <property type="term" value="C:cell cortex of cell tip"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061245">
        <property type="term" value="P:establishment or maintenance of bipolar cell polarity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.120.10.80">
        <property type="entry name" value="Kelch-type beta propeller"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015915">
        <property type="entry name" value="Kelch-typ_b-propeller"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052124">
        <property type="entry name" value="Rab9_kelch_effector"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46647">
        <property type="entry name" value="RAB9 EFFECTOR PROTEIN WITH KELCH MOTIFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46647:SF1">
        <property type="entry name" value="RAB9 EFFECTOR PROTEIN WITH KELCH MOTIFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24681">
        <property type="entry name" value="Kelch_KLHDC2_KLHL20_DRC7"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117281">
        <property type="entry name" value="Kelch motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0880">Kelch repeat</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="4">
        <location>
            <begin position="446"/>
            <end position="658"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="341"/>
            <end position="400"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="363"/>
            <end position="374"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP79711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="716" mass="81656" checksum="FB9EEC6CE7D5B199" modified="2011-01-11" version="1">MTEHNHVGQYYSNIDWTHHQLMYTFVTRHRIVKGFNQLQPPFPRTGASSAILRSLINPPTMFFFGGSSIQNQHNDLYRFTFDPNDSKPSLTAGIFNTRGQAPAPRSHAILSATDGHLFLWGGQTNSNVLDHHLYRLSLSTNYWNHYIISGPSPALLFGSSSTIFEGFLYVYASHDVNRSVQGDMWRIELDSLPSVPSWELISSSKDQICPPARTSHSAVVSSNSWIIFGGTDGVDVFNDLWEYRFREKKWRQVSANGSVPPPRKDHLVTQVKDHMYVFGGHDVSDHKIFDFFALDLINHVWHRLSSNGLEQVDPCGNISAWNQQLQQQNYISTSAMESLSSVEDYPTNEPPPGKRIRPQKQAKRWDRAREKREQPVCTPGQHQNIYHHSVPPSRQHDPCPTELVTSQRTIKEQYVSISHGVCIVAPNDLPPFCMAAWYPFETMAANEKKGWEQFVLHLLSRIDYVAQVNTNGPQQDGTMWADGWRKCSKNTEHFGRFCCVESLRKQMKLLNFDPDDQKARLLQAGEWISSHLKDFAPVVHDNYHQLLTINQYPSMNHTEYGQPYTTSDFASFLTFTMFDFHNLPHNDNDVNDWTLVGWIPIFNPKKSDNPQILADQDFDMIGGQFSFRDFQICLDLTKTLGVTLCVFRSKDFRHQTLPGASPSGKYTQIGFSCQINQSMASAVTAYLQGNYEKKLNIGGLQNQVDNANAPKKKKKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3K6J6</accession>
    <name>E3K6J6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF4219 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05381</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EFP80156.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324575.2">
        <property type="nucleotide sequence ID" value="XM_003324527.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K6J6"/>
    <dbReference type="STRING" id="418459.E3K6J6"/>
    <dbReference type="GeneID" id="10534335"/>
    <dbReference type="KEGG" id="pgr:PGTG_05381"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05381"/>
    <dbReference type="HOGENOM" id="CLU_051119_2_0_1"/>
    <dbReference type="InParanoid" id="E3K6J6"/>
    <dbReference type="OrthoDB" id="2498930at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990456">
        <property type="term" value="P:mitochondrion-endoplasmic reticulum membrane tethering"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015914">
        <property type="term" value="P:phospholipid transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027532">
        <property type="entry name" value="Mdm12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204:SF1">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP80156.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="250" mass="28484" checksum="21E363D4CA33A421" modified="2012-04-18" version="2">MTNSRTSPLLLNDTNYTTWSFLMTAKLSKLDVLEIVLGSIKKPELDDIKKPNDQYLAYCESNRVAYIEIIEHLNSHHLAYVAQILNDDNSFCRFSVWQILKKKYAGNNYSSKDTALEKFLNLEYHGSTSKFIYEARAANHRLTTAKVGLDDQVKTAIILCKLPSEFQSFRTVVSIGYPHDSVPTMLSRLEKHATQNHLDRSSTSIPSPQALLTTDEKFYMCPHCKKGFTICSHCNKAGHKESICFEKHPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K7B4</accession>
    <name>E3K7B4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphatidylglycerol/phosphatidylinositol transfer protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06400</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80444.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324863.2">
        <property type="nucleotide sequence ID" value="XM_003324815.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K7B4"/>
    <dbReference type="GeneID" id="10539453"/>
    <dbReference type="KEGG" id="pgr:PGTG_06400"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06400"/>
    <dbReference type="HOGENOM" id="CLU_095050_1_0_1"/>
    <dbReference type="InParanoid" id="E3K7B4"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171794" description="Phosphatidylglycerol/phosphatidylinositol transfer protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP80444.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="198" mass="21674" checksum="DD9670BA8E685C8D" modified="2012-04-18" version="2">MALIQFLITALLGLQVLAASPPKKPVDPTAPHCFTDMGEAQKTECPVALAKIIYNKDTMNKLENKLSVVSGDCYINVYNKKKVVVVVLTTSPSIGGPGNTEVTIWVTARFNPRNWYTPYDPDFPLEKIYCFHGAGVRCLIRLARAFNQIPVDGQGQLVSPTTKKLAQSLEIKAKACRVTAYTSDGTNVVLSAFELRIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K8T4</accession>
    <name>E3K8T4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06596</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80640.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325059.2">
        <property type="nucleotide sequence ID" value="XM_003325011.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K8T4"/>
    <dbReference type="GeneID" id="10539655"/>
    <dbReference type="KEGG" id="pgr:PGTG_06596"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06596"/>
    <dbReference type="HOGENOM" id="CLU_162175_0_0_1"/>
    <dbReference type="InParanoid" id="E3K8T4"/>
    <dbReference type="OrthoDB" id="192748at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP80640.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="126" mass="13920" checksum="2E1F9E21CFDF8CFA" modified="2012-04-18" version="2">MIQQPDSHPSTSAGKPRSEIYNLIPPKAPPNLTNPNLYAGRSTKPIPLELSLPLRWQRGAQFLAVSLSITCGVWGVLFADFGNGDREIVFSPIRRWFAPYKSLIFSADQGSTDVQPDNVLPKVESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3K9S0</accession>
    <name>E3K9S0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Methyltransferase-like protein 14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_07393</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MT-A70-like family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP81141.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325560.2">
        <property type="nucleotide sequence ID" value="XM_003325512.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K9S0"/>
    <dbReference type="SMR" id="E3K9S0"/>
    <dbReference type="STRING" id="418459.E3K9S0"/>
    <dbReference type="GeneID" id="10536544"/>
    <dbReference type="KEGG" id="pgr:PGTG_07393"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07393"/>
    <dbReference type="eggNOG" id="KOG2097">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_429020_0_0_1"/>
    <dbReference type="InParanoid" id="E3K9S0"/>
    <dbReference type="OrthoDB" id="14833at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036396">
        <property type="term" value="C:RNA N6-methyladenosine methyltransferase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016556">
        <property type="term" value="P:mRNA modification"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045123">
        <property type="entry name" value="METTL14-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007757">
        <property type="entry name" value="MT-A70-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13107">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13107:SF0">
        <property type="entry name" value="N6-ADENOSINE-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05063">
        <property type="entry name" value="MT-A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51143">
        <property type="entry name" value="MT_A70"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51592">
        <property type="entry name" value="SAM_MTA70L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="447"/>
            <end position="480"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="526"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="448"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="459"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="556"/>
            <end position="566"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="573"/>
            <end position="591"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="592"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="622"/>
            <end position="643"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP81141.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="643" mass="72386" checksum="B46053E4590BA709" modified="2012-04-18" version="2">MSHHDQLTHSPSPRPKSAISAREIQQLSSKKRHKRLAYGPNYIPEEETIRNDYSLNYVLTGSRPQNHIQNTALETRFAEYPKLKKLLQLKRSLVTSHAHPPTYIQADLRPNRPDSFNLLELSPARFDVILLDPPLKEYVENYPALSNEPSIAGCGGPPDQPRDYWDWDELENLPLPQIAASPGFVFLWCGSGQGSTLERGRALLAKWGYRKCEDLVWIKTNSTQIGSPQPSAIPAVFTPTKEHCLMGIRGTVRRSTDGHFVHCNVDTDVIVSEPDPTDVLHKPEELYQLIENFCLGRRRLELFGSSRCLRPGWLTIGSHCVPPTIKTDEQALDPHLRPVPYSQAEYLSHFLDSAGRTHNLLPSHADIDNLRPRSPPSRITTAAITGPGLAPGMGIGYAVPVGMPIVGPGGTIVTTTISGRNTLSHPSGGLGRGPGNALLPGMRVSGLRDSNNPIHNRANNNNNNQQQQQQPFGPHANTFFHHNFPLSPSYPFLQTSHYPTHQSWTGHPQLIYPHQHFQAGQNAYGMHQPQPIYYHPPNPSQQPFNQQFQNHHHHQQQQQQQQPNPSRQYPAYHQSNSPVDPASAASNWTFSNYPQPHHQNYQQQPPQPFYPSYAAQHPNPPSNQHQQQQQQSHQQQHQQQQQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>E3K9V8</accession>
    <name>E3K9V8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">4-aminobutyrate aminotransferase</fullName>
            <ecNumber evidence="3">2.6.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">GABA aminotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Gamma-amino-N-butyrate transaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_07214</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23352"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57706"/>
            <dbReference type="ChEBI" id="CHEBI:59888"/>
            <dbReference type="EC" id="2.6.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.19" evidence="3"/>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP80962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325381.1">
        <property type="nucleotide sequence ID" value="XM_003325333.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K9V8"/>
    <dbReference type="FunCoup" id="E3K9V8">
        <property type="interactions" value="200"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K9V8"/>
    <dbReference type="GeneID" id="10536120"/>
    <dbReference type="KEGG" id="pgr:PGTG_07214"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07214"/>
    <dbReference type="eggNOG" id="KOG1405">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016922_12_0_1"/>
    <dbReference type="InParanoid" id="E3K9V8"/>
    <dbReference type="OMA" id="GLMCAFD"/>
    <dbReference type="OrthoDB" id="10260828at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034386">
        <property type="term" value="F:4-aminobutyrate:2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009450">
        <property type="term" value="P:gamma-aminobutyric acid catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000073">
        <property type="entry name" value="Probable 4-aminobutyrate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004631">
        <property type="entry name" value="4NH2But_aminotransferase_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00699">
        <property type="entry name" value="GABAtrns_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43206:SF1">
        <property type="entry name" value="4-AMINOBUTYRATE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43206">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 13" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00031787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP80962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="528" mass="58839" checksum="7F27A19036323D07" modified="2011-01-11" version="1">MSYPIRSTISPVRFLRPLLFLSRPAPRHHHLTLRQSLDQTRTRAGAAKRTYAVQASPRFFAKEPSGPQVSTSEIPGPASRKLSREINEWQDARAHQMVADYSKSIGNYLQDVDGNRLLDVFAQIASIAIGYNHPKLLELARSDEFVQLAMNRPALGSFPPANWDHIVSTGLLKVAPQGLNQLFTMMCGSCANEGALKAAFFAYRQRERGGGLSQFSAEELKSCMNNTQPGSPDLVAMSFRSGFHGRLFGSLSLTRSKAIHKIDVPAFDWPAVEFPRLKYPLADHEAENKKIEEDAISKVEETIVEWNAKGRSVAALIVEPIQSEGGDFHASPAFFRKLREVTLKHHIYLIVDEVQTGVAATGHFWAHEKWGLTTPPDFVTFSKKMQAAGFFHAPETRPTQPYRNYNTWMGTPTEILKARAIIEVIQDQGLIEHVSSVGKYLYQGLDELSKSVGQGKIINLRGQDSGTFLAFDCSSSETRDRFVQQMKLAGVNVGGCGDQTVRLRPMLVFEKIHADIFLNTAEKVLKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBB6</accession>
    <name>E3KBB6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">Ubiquitin-like protein ATG12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_07878</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Ubiquitin-like protein involved in cytoplasm to vacuole transport (Cvt), autophagy vesicles formation, mitophagy, and nucleophagy. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate functions as an E3-like enzyme which is required for lipidation of ATG8 and ATG8 association to the vesicle membranes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 8">Forms a conjugate with ATG5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Preautophagosomal structure membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the ATG12 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326048.1">
        <property type="nucleotide sequence ID" value="XM_003326000.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KBB6"/>
    <dbReference type="FunCoup" id="E3KBB6">
        <property type="interactions" value="202"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KBB6"/>
    <dbReference type="GeneID" id="10533172"/>
    <dbReference type="KEGG" id="pgr:PGTG_07878"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07878"/>
    <dbReference type="eggNOG" id="KOG3439">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_106795_3_0_1"/>
    <dbReference type="InParanoid" id="E3KBB6"/>
    <dbReference type="OMA" id="YAKTHAW"/>
    <dbReference type="OrthoDB" id="10003551at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034274">
        <property type="term" value="C:Atg12-Atg5-Atg16 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000421">
        <property type="term" value="C:autophagosome membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034045">
        <property type="term" value="C:phagophore assembly site membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031386">
        <property type="term" value="F:protein tag activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000045">
        <property type="term" value="P:autophagosome assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097352">
        <property type="term" value="P:autophagosome maturation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000422">
        <property type="term" value="P:autophagy of mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061723">
        <property type="term" value="P:glycophagy"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034727">
        <property type="term" value="P:piecemeal microautophagy of the nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01612">
        <property type="entry name" value="Ubl_ATG12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.90:FF:000150">
        <property type="entry name" value="Ubiquitin-like protein ATG12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007242">
        <property type="entry name" value="Atg12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029071">
        <property type="entry name" value="Ubiquitin-like_domsf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13385">
        <property type="entry name" value="AUTOPHAGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13385:SF0">
        <property type="entry name" value="UBIQUITIN-LIKE PROTEIN ATG12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04110">
        <property type="entry name" value="APG12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54236">
        <property type="entry name" value="Ubiquitin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0072">Autophagy</keyword>
    <keyword evidence="4 8" id="KW-1017">Isopeptide bond</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <keyword evidence="5 8" id="KW-0833">Ubl conjugation pathway</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00015875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022499"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU361201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP81629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="114" mass="12570" checksum="FD4F7EBA9F51CB9B" modified="2011-01-11" version="1">MSSTHFPTTGLAEATKALEEFKQRDASKVVVRFKATGSAPIMKQNFFKITSSNRFQAVIAFLRKELGLKPTDPVFLYINSSFSPAPDETVANLFKCFSTDGHLIVNYSSTAAWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCV7</accession>
    <name>E3KCV7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_07640</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82243.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326662.2">
        <property type="nucleotide sequence ID" value="XM_003326614.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCV7"/>
    <dbReference type="GeneID" id="10529616"/>
    <dbReference type="KEGG" id="pgr:PGTG_07640"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07640"/>
    <dbReference type="HOGENOM" id="CLU_130010_0_0_1"/>
    <dbReference type="InParanoid" id="E3KCV7"/>
    <dbReference type="OrthoDB" id="2508525at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP82243.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="118" mass="13778" checksum="63552222C0257563" modified="2012-04-18" version="2">MSQSPKSHRKYTAKELEIEEHYEWLHETHADMVKVVQMWEPLVYRDTDTLVCSIITPSSKYFRPMIDCTHWFAAKDIQLKADGEAADLGIPQCEVPNIMEIWAQRRAELARGKSIHLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KD32</accession>
    <name>E3KD32_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82283.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326702.2">
        <property type="nucleotide sequence ID" value="XM_003326654.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KD32"/>
    <dbReference type="GeneID" id="10529656"/>
    <dbReference type="KEGG" id="pgr:PGTG_07680"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07680"/>
    <dbReference type="HOGENOM" id="CLU_1038764_0_0_1"/>
    <dbReference type="InParanoid" id="E3KD32"/>
    <dbReference type="OrthoDB" id="2499492at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="123"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82283.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="308" mass="34309" checksum="F6853DC48D306952" modified="2012-04-18" version="2">MVSRLKNLFAKSKSGEAKFSRRLDAIFCRGNRTSSSQSISSKNKELERTSSRATIDSKINVNNRRLQIRLSVTRTTRYSPVHCSPCSPPAEGTRLEQALPLLPTEIRKESETRRASDLPRSPSPIATSHESSSSESQSTDCHYFENVKSETSYGSSEILEAQEEAVRIKFRDAYFPAPSTTCTEKTNLKLSRNLMDALSSHQIEWAAMIELQKADHSGILRGLTSRQIRWLVREGRCKTWLVGTAGPLIEEEKDEVQDVQSIDSSDGVNLTEPISEEAVVLCARKAISPMDQLPAVLGVRSTCTDSRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KE91</accession>
    <name>E3KE91_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08820</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82624.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327043.2">
        <property type="nucleotide sequence ID" value="XM_003326995.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KE91"/>
    <dbReference type="GeneID" id="10530007"/>
    <dbReference type="KEGG" id="pgr:PGTG_08820"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08820"/>
    <dbReference type="HOGENOM" id="CLU_2185257_0_0_1"/>
    <dbReference type="InParanoid" id="E3KE91"/>
    <dbReference type="OrthoDB" id="2516221at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82624.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="109" mass="11991" checksum="AB39FCEB039BA7A0" modified="2012-04-18" version="2">MHYSYLQLATCIICSLQVYKLHATGTVKEPGFVGTDLERSFVEETTTATINTAQRLPNKENASKTPKQSLQDTISASSSQSLLEAFHLENKTSTKGKQPRMKTAETPQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3KED6</accession>
    <name>E3KED6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08865</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82669.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003327040.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KED6"/>
    <dbReference type="GeneID" id="10530052"/>
    <dbReference type="KEGG" id="pgr:PGTG_08865"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08865"/>
    <dbReference type="HOGENOM" id="CLU_575073_0_0_1"/>
    <dbReference type="InParanoid" id="E3KED6"/>
    <dbReference type="OrthoDB" id="10280627at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
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    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="264"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="20"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82669.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="478" mass="54318" checksum="E6DFCD62AB7ECDA5" modified="2012-04-18" version="2">MVVMTDPNAESTVSSDSTPADNCDAREDTITIIRELTRVWKRHREPIDEKIASTKEALSIDESKALLFQLHTSILPILRQQIKELFSSDPDDQSDFAKKPHLYKHVDILGMTAKIDHTLDQIRRSVEHIACETRQSPSNNDREWGALKRYRTVALLERINSLLTSTSSFFPHYILLTWPNTDNRSLDRGHDFDDQSDSYDPREILVRKTDSYLDCIDRIIDSYHQSDLVYLRKGWQWCAEEFESYLPKIIGPINSAISEAKGHLSKPASTLQDDQPEAETDRSEVDSTDGSEDDRSSNSDVNNESESSRTYFVPIAQSVIPIIKLNRLLFKKLSGSTSSIQRLLTIDDTMLSSQEIESLKKQSELLPIYVNSLVEAILHIYNRSEAGGGLNYLQSLADLISNNFHSTINLITRHLVSSTLQRLADHPPSKDLIILKTWLLTVANQFAVASCNYRKVVTESIINRQLALVDIQVHMLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGI4</accession>
    <name>E3KGI4_PUCGT</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <name evidence="2" type="ORF">PGTG_08595</name>
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83409.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="AlphaFoldDB" id="E3KGI4"/>
    <dbReference type="STRING" id="418459.E3KGI4"/>
    <dbReference type="GeneID" id="10545126"/>
    <dbReference type="KEGG" id="pgr:PGTG_08595"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08595"/>
    <dbReference type="HOGENOM" id="CLU_004591_6_0_1"/>
    <dbReference type="InParanoid" id="E3KGI4"/>
    <dbReference type="OrthoDB" id="2506745at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="503"/>
            <end position="513"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83409.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="513" mass="59111" checksum="F7E6520C067E9142" modified="2012-04-18" version="2">MVENIRGKNKAEWKSEVEAIEKDNDERLFNPIYRLKGFDGCKDTPVEVLHVFLLGVVKYMTQDFMKGLKVYQISELIVRWEAFNTDALNHPSISPSYVVKHYKSLIGKDFKIILQAAPFVFFDFMSTAQKGHWFSLCILSTYIFQSNIADMATHLADLRKYIDIFLFHTIQQSARWVNKPKFHMLLHLPESVERFGPAGLFATEQFESFNKILRLASIHSNRHNPGRDISISFADFECLRAIFSGGRLINHRSGQTFFASPNVTNIFRDNSLIRRSMGYNDNMVADSHVYPMEMITPVPQEEKEPPPHYLQEHLAGYHFTQVHQLHINSKDVCKKGYFVVIFPRNKSPKFIGRVESIWKGQFQNRKTLYVKITRFQVKSIDQFYNMTALVNEKTIEFYLASEVAGCLNVQHDCHLGKCMPDKNNKRKFGPEEAAPSKEAVKHQDDHSYIINSASLHSIPLRQQFSNMTRPPITPKAWTKCVQDGLKNWFEPSKGKAKAPKASQVASTSQMMLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KKL4</accession>
    <name>E3KKL4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_10310</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178292">
        <property type="protein sequence ID" value="EFP84839.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329258.2">
        <property type="nucleotide sequence ID" value="XM_003329210.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KKL4"/>
    <dbReference type="GeneID" id="10526813"/>
    <dbReference type="KEGG" id="pgr:PGTG_10310"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10310"/>
    <dbReference type="HOGENOM" id="CLU_792587_0_0_1"/>
    <dbReference type="InParanoid" id="E3KKL4"/>
    <dbReference type="OrthoDB" id="10374135at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172681" evidence="2">
        <location>
            <begin position="23"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP84839.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="350" mass="40067" checksum="F0B883D649ADEE88" modified="2012-04-18" version="2">MYLTRMMHINLLGLSISAQISCGMVKSWPGQKCDGSSDMVDLDLESLRSDWNESGYGSSSLGQPSRMGRSKEIPNCGLEAEPDTGLSLLTLHHKDLLSTGRDVDIPGIENGGLENLKKCASKTLSSMRSLRAEFSNLKDTESERRKIGHHMDEVERAVRNLFHRRIIELQDKPYLFLGHSNEPIADDNYQEIFKMAGKEWLKTAANLKAFSQLLDIKDHPKRRVMFKKWCDIMMKFFVDLKKHKVIHAENLAEFLNKDDQGKFIGFYIYERSFPEHHLHAAYLTFNIRLSLQEGSPLMNAQYVSNSSMLKSRESTKTSTSLRLRDTFMSSIHKSILQNHCNQRSSLSLNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLK5</accession>
    <name>E3KLK5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_11349</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the WD repeat MORG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP85180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329599.1">
        <property type="nucleotide sequence ID" value="XM_003329551.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLK5"/>
    <dbReference type="FunCoup" id="E3KLK5">
        <property type="interactions" value="150"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KLK5"/>
    <dbReference type="GeneID" id="10527163"/>
    <dbReference type="KEGG" id="pgr:PGTG_11349"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11349"/>
    <dbReference type="eggNOG" id="KOG0316">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_57_1_1"/>
    <dbReference type="InParanoid" id="E3KLK5"/>
    <dbReference type="OMA" id="MCWDIRT"/>
    <dbReference type="OrthoDB" id="1068471at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000398">
        <property type="term" value="P:mRNA splicing, via spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00200">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020472">
        <property type="entry name" value="WD40_PAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051980">
        <property type="entry name" value="WD_repeat_MORG1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22842:SF3">
        <property type="entry name" value="WD REPEAT DOMAIN-CONTAINING PROTEIN 83"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22842">
        <property type="entry name" value="WD40 REPEAT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00320">
        <property type="entry name" value="GPROTEINBRPT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="3 6" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="15"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="57"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="99"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="repeat" description="WD" evidence="6">
        <location>
            <begin position="269"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP85180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="304" mass="33826" checksum="F6FE2187291C6042" modified="2011-01-11" version="1">MSSQLAHTSPALQCQLEHKGIVHVATFNHGAQYVLTGGQDRTIRLWNAKTGNFIKDYSGHGYEILGIAVSPNNSFLGSVGGDRSVFYWDVSTGAILRRFSGHTGVINACAFNFDGNLLISGSFDASVRVWDIKSQQRHPIQIFQDAKDSITSIIVSGSEFITGSVDGYVRSYDIRMGQMMEDYFEDPVTSIHLLKDSKTMAVSTLNSTIRLMDRERGEMLQKFTGHRALNYRSRISMSYDEAQVLAGDEDGLLWAWDLESGKKNAGMSFEAHQKHVTWVECHPKTNCFITAAADGLTRLWNLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNN7</accession>
    <name>E3KNN7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">NuA4 histone acetyltransferase subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_11668</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the actin family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178297">
        <property type="protein sequence ID" value="EFP85912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330331.1">
        <property type="nucleotide sequence ID" value="XM_003330283.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNN7"/>
    <dbReference type="FunCoup" id="E3KNN7">
        <property type="interactions" value="180"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KNN7"/>
    <dbReference type="GeneID" id="10539911"/>
    <dbReference type="KEGG" id="pgr:PGTG_11668"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11668"/>
    <dbReference type="eggNOG" id="KOG0679">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027965_6_2_1"/>
    <dbReference type="InParanoid" id="E3KNN7"/>
    <dbReference type="OMA" id="MTEAPWN"/>
    <dbReference type="OrthoDB" id="5132116at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035267">
        <property type="term" value="C:NuA4 histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016514">
        <property type="term" value="C:SWI/SNF complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006338">
        <property type="term" value="P:chromatin remodeling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd13395">
        <property type="entry name" value="ASKHA_NBD_Arp4_ACTL6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000528">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.640.10:FF:000133">
        <property type="entry name" value="SWI/SNF and RSC complexes subunit arp42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.640.10">
        <property type="entry name" value="Actin, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004000">
        <property type="entry name" value="Actin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020902">
        <property type="entry name" value="Actin/actin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11937">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00022">
        <property type="entry name" value="Actin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00190">
        <property type="entry name" value="ACTIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00268">
        <property type="entry name" value="ACTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01132">
        <property type="entry name" value="ACTINS_ACT_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="232"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="259"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP85912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="469" mass="51155" checksum="2E7904E2EB80ACAF" modified="2011-01-11" version="1">MPTYGGDEVSAVVLDIGSSTTRAGYAGEDTPKVVIPTSYGYIPPASDSAEPGAGSYYFGDHGPTLWRAQMEVRSPMKESMVEDWTAVGKLITTVFDREMRLGLPAAVLEPDEKRGILTEHPLLVTEPSWNSKENRERMIEMAFEELDTPAYYSVDRSVMAAFAAGKGTAVVVDIGDDLTTVTPICDGFVLRKGIQKSPIAGNVLSSLLLSTTESLLPTPLYPHSLIASKASHPAPVDPDSAMASATDEDPVLVPPGVTLRDERLPGKENSKTTDSFLRYQKIRIMHDLKESICEVFPRTWEEETLSAMPGRTFEFPSGQQQQYGRERYMIPEVIFNPAISPWPLGKHVGGRHPGSTISMAEAPLPLSHLLLEAIAASDIDIQPTLNANIILTGASSLIPGLIDRLDFDLKAISPAVKFKISAPGNLVERKFAPWLGGSILASLGSFHQLWIGKDEYKEQGRSVVHRRCK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPG8</accession>
    <name>E3KPG8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">N-acetylglucosaminylphosphatidylinositol deacetylase</fullName>
            <ecNumber evidence="2">3.5.1.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_12159</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the PIGL family.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.89" evidence="2"/>
    <dbReference type="EMBL" id="DS178299">
        <property type="protein sequence ID" value="EFP86203.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330622.2">
        <property type="nucleotide sequence ID" value="XM_003330574.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KPG8"/>
    <dbReference type="FunCoup" id="E3KPG8">
        <property type="interactions" value="263"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KPG8"/>
    <dbReference type="GeneID" id="10540213"/>
    <dbReference type="KEGG" id="pgr:PGTG_12159"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12159"/>
    <dbReference type="eggNOG" id="KOG3332">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034979_0_2_1"/>
    <dbReference type="InParanoid" id="E3KPG8"/>
    <dbReference type="OrthoDB" id="440160at2759"/>
    <dbReference type="UniPathway" id="UPA00196"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000225">
        <property type="term" value="F:N-acetylglucosaminylphosphatidylinositol deacetylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006506">
        <property type="term" value="P:GPI anchor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10320">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003737">
        <property type="entry name" value="GlcNAc_PI_deacetylase-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024078">
        <property type="entry name" value="LmbE-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993:SF11">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12993">
        <property type="entry name" value="N-ACETYLGLUCOSAMINYL-PHOSPHATIDYLINOSITOL DE-N-ACETYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02585">
        <property type="entry name" value="PIG-L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102588">
        <property type="entry name" value="LmbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="102"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="53"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="57"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP86203.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="402" mass="45910" checksum="5B7A9ED67C4DAAF3" modified="2012-04-18" version="2">MSASQSKNRTNQEEEDIPPISPLLEPSYQYPSRDNQIREFDYGDREDVLAEAEELDSTNSHYPPSSKRSANNNNHHHNRTTTKTIINALSLGLRKHRTKFRLLVVFLTLWPLLCWTGIFLLFSDHPALFPSSLRGSKSTLFVVAHPDDECLFFAPSILATVQRAKSHGALLVMSSGNHYGQGGLRRKELLGSCKQLGIREDRCDVLDISQIQDDPIIWWPVDRIGKLVNEHIERWMIDSIVTFDDYGVSGHINHRAVSASVTELAISLYKKSVAPNHSSTKSPKLYRVKSVFVLRKYIGLFDLPLSFIRLIPSIIFGPSQQINSALQSLTYDPKTEQTTQESERQRRAVTTTFEKGLLISGLSGYRSARNAFWSHQSQMVWDRHLYMILSQFMYFNTIERIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQN6</accession>
    <name>E3KQN6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_12993</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178301">
        <property type="protein sequence ID" value="EFP86611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331030.1">
        <property type="nucleotide sequence ID" value="XM_003330982.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQN6"/>
    <dbReference type="STRING" id="418459.E3KQN6"/>
    <dbReference type="GeneID" id="10540711"/>
    <dbReference type="KEGG" id="pgr:PGTG_12993"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12993"/>
    <dbReference type="HOGENOM" id="CLU_897524_0_0_1"/>
    <dbReference type="InParanoid" id="E3KQN6"/>
    <dbReference type="OrthoDB" id="10277660at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP86611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="329" mass="37176" checksum="A34392A11CD8146D" modified="2011-01-11" version="1">MTPQFDPTGPVFFAYGTLTMHDEADTQSRTRPEEAEAIEIPPLMGNTLTEAELQEFYPELAEWPCTTTASEDECPVTSVPPVVPAPLSVVIILEYHCWLPSPQADQDGCRQYRAAHGVHTWTETMGPSRLVTWRGDPRASTWDQIRHDVLEAIGSYDSEVADELRSNDRYRDLLWEGTIPDLREFGRGVPFRVCTDVCWEIFTGYIARTTATSKIYLRLFTNRPYGRTAVSDAFCKAMHIRHCPHRNNLIAELERRLHTEATHLDGNSGHIQLKVKWAPGGQKFIRMSAAVIHGWARAIVSNPSLVTLDTPPVGREYLWEGVHAHAQLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KR03</accession>
    <name>E3KR03_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178302">
        <property type="protein sequence ID" value="EFP86728.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331147.2">
        <property type="nucleotide sequence ID" value="XM_003331099.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KR03"/>
    <dbReference type="GeneID" id="10541152"/>
    <dbReference type="KEGG" id="pgr:PGTG_13110"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13110"/>
    <dbReference type="HOGENOM" id="CLU_161519_0_0_1"/>
    <dbReference type="InParanoid" id="E3KR03"/>
    <dbReference type="OrthoDB" id="10270463at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173808" description="Secreted protein" evidence="1">
        <location>
            <begin position="20"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86728.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="13978" checksum="B704D7A6F83629AB" modified="2012-04-18" version="2">MKLIHILALALVLTGISIAVDPVLCEGCGHRTGHLIGEVEEKCGQYDKKDPTSVACTYLRQKKVQECSRKECGQVFHMNIPIKKNGGTPLLFRQCKHQWSQPASRPTATTRSTHMYNFMPGAPRSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>E3KR12</accession>
    <name>E3KR12_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_13119</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178302">
        <property type="protein sequence ID" value="EFP86737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331156.1">
        <property type="nucleotide sequence ID" value="XM_003331108.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KR12"/>
    <dbReference type="GeneID" id="10541161"/>
    <dbReference type="KEGG" id="pgr:PGTG_13119"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13119"/>
    <dbReference type="HOGENOM" id="CLU_2016370_0_0_1"/>
    <dbReference type="InParanoid" id="E3KR12"/>
    <dbReference type="OrthoDB" id="10548686at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP86737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="123" mass="13895" checksum="740F3158A3697AEE" modified="2011-01-11" version="1">MGTQGVLSPLLGRFDDGLTLVSLLEGMRTKLGLTKTRRESNFAADSVRPTEGNIKSRSDLKMECGHANCEKTSDEEVRIPESAYNIHYRRLQESPVLVTYPITRNMQRVGHSRRRSRPTCYGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KR40</accession>
    <name>E3KR40_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13147</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178302">
        <property type="protein sequence ID" value="EFP86765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331184.1">
        <property type="nucleotide sequence ID" value="XM_003331136.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KR40"/>
    <dbReference type="GeneID" id="10541190"/>
    <dbReference type="KEGG" id="pgr:PGTG_13147"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13147"/>
    <dbReference type="HOGENOM" id="CLU_1741462_0_0_1"/>
    <dbReference type="InParanoid" id="E3KR40"/>
    <dbReference type="OrthoDB" id="10268594at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172148" evidence="1">
        <location>
            <begin position="18"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="150" mass="17114" checksum="8BDAC3DBBB052AF4" modified="2011-01-11" version="1">MRLVGLIVLATLKHCAGVEPPDECLTWHRTVCVRTDLSWKVNGNTANLHCDTCHHDSPSFQRKPCPRDGGILEPMYGNRQCAYCGYRVEKGAHSMICKTCNLSPIEYTLQRKACDQCGRRPLTMSEIVKIYDDNVLPRDRNTYRIDRPFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>E3KRZ5</accession>
    <name>E3KRZ5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_13289</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EFP87070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331489.1">
        <property type="nucleotide sequence ID" value="XM_003331441.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KRZ5"/>
    <dbReference type="GeneID" id="10542151"/>
    <dbReference type="KEGG" id="pgr:PGTG_13289"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13289"/>
    <dbReference type="HOGENOM" id="CLU_2321513_0_0_1"/>
    <dbReference type="InParanoid" id="E3KRZ5"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP87070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="99" mass="11225" checksum="01FE5A1A5AF52959" modified="2011-01-11" version="1">MVRMNSAARVLAVEAGGAVKLLRWKSVVICQFPRFGEFLICLHKPLILQLSCSLQRASRLVKPNNPLRRTLDPRISRPVIGSKRKLPSFLPYYIHSGKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3KSL9</accession>
    <name>E3KSL9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CCAAT-binding factor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_12889</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CBF/MAK21 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178305">
        <property type="protein sequence ID" value="EFP87305.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331724.2">
        <property type="nucleotide sequence ID" value="XM_003331676.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KSL9"/>
    <dbReference type="STRING" id="418459.E3KSL9"/>
    <dbReference type="GeneID" id="10537705"/>
    <dbReference type="KEGG" id="pgr:PGTG_12889"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12889"/>
    <dbReference type="eggNOG" id="KOG2154">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015945_1_0_1"/>
    <dbReference type="InParanoid" id="E3KSL9"/>
    <dbReference type="OrthoDB" id="10263185at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030692">
        <property type="term" value="C:Noc4p-Nop14p complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032040">
        <property type="term" value="C:small-subunit processome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005612">
        <property type="entry name" value="CCAAT-binding_factor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027193">
        <property type="entry name" value="Noc4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12455">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12455:SF0">
        <property type="entry name" value="NUCLEOLAR COMPLEX PROTEIN 4 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03914">
        <property type="entry name" value="CBF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CCAAT-binding factor" evidence="3">
        <location>
            <begin position="447"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="254"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="302"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="270"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007797"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP87305.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="684" mass="77276" checksum="AE29978AEF950C28" modified="2012-04-18" version="2">MAIKSIPTKTNSTPTTTNKQHQKTIKQTLKQIKETEQQLKQTTNQDLNPIADLLERLQKEVKAATEEEEEQNQKTKKQATTHQVTHALIYALHRIFSSLIRQGRIHGSPTRTKAQEGPEEEAIKLVKEWLKTQYSQYIDCLLVLLHLSTTQEEHGTQIELDALTILINLVRAESDIIRALKYPSLPEISAKFPPAGFESATFLKLVKTLLIPTLKLPPIPQSVRAEFILRYLNFCDDLRFRFLKDATFLCQSSNPESIQQGRKRKKAKTTVPTDNSNADQNKGLTRNLLMYLESLTTMPTREEELDQFWTGKPEETSAGSMRTRKRTRGGKEGAGGQKKKTQKTADGSTGIFDDPSDSEASGPEALELAQEPRGLGKNQQHPLLSLRAHQKAFSDCWIAFLSRQDLAESDIKRILNLLHDQVIPHMIDPKILMDFLVDCLDYGGSISVLSLNALFTLISKHNLDYPDFYTRLYALLDSSIMHTRHRPRFFRMLEVFLSSTHLPVNIVASFVKKIARLSLFAPPAATITVVPFCYNLIKLHPTVMALLHRLPDPNSKSLKALPINDPFKLDEPDPLKTDAIFSSAWELVGLRSHYLASISTLFKVFQESFDKPKYDLEDFLDHSYSTLIDTELTRMIKKPPALSLFSISYKEPASSSSSLTTSAPLGSKKSPVVVDDIVNQMWKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3KUZ6</accession>
    <name>E3KUZ6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Cytochrome c oxidase assembly protein cox15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_12543</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Fe(II)-heme o + 2 A + H2O = Fe(II)-heme a + 2 AH2</text>
            <dbReference type="Rhea" id="RHEA:63388"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:60530"/>
            <dbReference type="ChEBI" id="CHEBI:61715"/>
            <dbReference type="EC" id="1.17.99.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:63389"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>heme b</name>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Porphyrin-containing compound metabolism; heme A biosynthesis; heme A from heme O: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178311">
        <property type="protein sequence ID" value="EFP88096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332515.1">
        <property type="nucleotide sequence ID" value="XM_003332467.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KUZ6"/>
    <dbReference type="FunCoup" id="E3KUZ6">
        <property type="interactions" value="521"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KUZ6"/>
    <dbReference type="GeneID" id="10535238"/>
    <dbReference type="KEGG" id="pgr:PGTG_12543"/>
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    </dbReference>
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        <property type="entry name" value="HemeA_Synthase_type2"/>
    </dbReference>
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        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23289:SF2">
        <property type="entry name" value="CYTOCHROME C OXIDASE ASSEMBLY PROTEIN COX15 HOMOLOG"/>
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    </dbReference>
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    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="5 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="185"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
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            <begin position="320"/>
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        </location>
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    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="405"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
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            <begin position="437"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="468"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001970"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
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        </source>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP88096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="15">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="513" mass="57135" checksum="ABD075370DEB2D93" modified="2011-01-11" version="1">MSSPHFLRLPLPSITKQIFSRRSLSGKFKLSPFSTCNQPSHRPSAFTSNLRLQRSPSISFTGNARPSSYSTPYDRCRIAFSRSNSSLSSNPELLLSPPIISKHLIFIAGLVFTIVVVGGYTRLTESGLSITEWNLVSGILPPLNAEDWEKEFAKYRATPEFKLLNHAIGLEEFKKIFFWEWAHRIMGRVIGLSFLLPIPIFIRYGLIRTKKTGWSLAGIGGLIGLQGGLGWYMVQSGLDQKELDQRDGVPRVSQYRLAAHLLMAFTVYSACIRLAGGLWRDWKVAVLDRPLFQHLNRHKISSALESIQLLNNKLPTRARFFIGSLTGLIFLTAGSGAFVAGLDAGLVYNTFPKMGDRWMPPQDELFSELYTNRGKQGSTNAEGGRSIVDLSWMRNIFENPTTVQFNHRVLGTLTFISSLSWAVFIEKNKHLVPRSTLNLSRLIALVASNQVGLGIATLVYLVPTNLALLHQANSLLLLSVSLFAGLSLRRPGALALKSVYKHSQTLLHKPKTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KWD1</accession>
    <name>E3KWD1_PUCGT</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_14811</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178315">
        <property type="protein sequence ID" value="EFP88606.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003332977.2"/>
    </dbReference>
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    <dbReference type="GeneID" id="10541234"/>
    <dbReference type="KEGG" id="pgr:PGTG_14811"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14811"/>
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    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
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        </location>
    </feature>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
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        <location>
            <begin position="11"/>
            <end position="30"/>
        </location>
    </feature>
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        <location>
            <begin position="68"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="87"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP88606.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="213" mass="23271" checksum="51A1F5A639B0B440" modified="2012-04-18" version="2">MSSIFSRRHGQTFTRTGRSTPDLLSSNASKLEQPKPPVNSLYHLDVVDASSVIIPSVITYSSPQTPEHSMSPASSSSHKSVPPRSHTVQPTTNNISFPTMNPSSAGPSAAQRPKRAPTPPPPYVDNFPSRDGADHSPVTPVACRSRSRTPSASTWIKRVVSRSSSRASLRKQYEQIPMSEEERIEAEQVKARKAKEALAEWNRINDALKAAGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3KWI7</accession>
    <name>E3KWI7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nudix hydrolase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_14746</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178316">
        <property type="protein sequence ID" value="EFP88780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333199.1">
        <property type="nucleotide sequence ID" value="XM_003333151.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KWI7"/>
    <dbReference type="FunCoup" id="E3KWI7">
        <property type="interactions" value="484"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KWI7"/>
    <dbReference type="GeneID" id="10541732"/>
    <dbReference type="KEGG" id="pgr:PGTG_14746"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14746"/>
    <dbReference type="eggNOG" id="KOG3041">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_062658_0_2_1"/>
    <dbReference type="InParanoid" id="E3KWI7"/>
    <dbReference type="OMA" id="WHWAAGW"/>
    <dbReference type="OrthoDB" id="10249920at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047631">
        <property type="term" value="F:ADP-ribose diphosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006753">
        <property type="term" value="P:nucleoside phosphate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019693">
        <property type="term" value="P:ribose phosphate metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18888">
        <property type="entry name" value="NUDIX_ADPRase_Nudt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.79.10:FF:000016">
        <property type="entry name" value="ADP-sugar pyrophosphatase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839:SF1">
        <property type="entry name" value="ADP-SUGAR PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11839">
        <property type="entry name" value="UDP/ADP-SUGAR PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="3">
        <location>
            <begin position="112"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="32"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP88780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="268" mass="29616" checksum="467488BB04C72661" modified="2011-01-11" version="1">MITRMIPPLTTPGLRLSGARCNYRAKWTGRWARNTRPSEPTKRTAMMSSTSTDDNKRPSFVLGSAAVTHSEPLENSKAKWIGLRSIDWIDETGKERRWEASYRKTAGESASDKPDAVAIFTLVSRPQLPLSTILVLQFRPPVNSIVVELPAGLIDENESAETAALRELREETGYGNGPNGGLVKVEEISEILVNDPGMSSANMVLAKVSVSLDRADVIPEPQQEQGEHIQVELVPLKSLYKTLQQFNQRPGYVVDARLMHLALGLTIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>E3KWZ6</accession>
    <name>E3KWZ6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATP-dependent Clp protease ATP-binding subunit ClpX</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_14228</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178317">
        <property type="protein sequence ID" value="EFP88889.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333308.2">
        <property type="nucleotide sequence ID" value="XM_003333260.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KWZ6"/>
    <dbReference type="STRING" id="418459.E3KWZ6"/>
    <dbReference type="GeneID" id="10542166"/>
    <dbReference type="KEGG" id="pgr:PGTG_14228"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14228"/>
    <dbReference type="eggNOG" id="KOG0745">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014218_1_2_1"/>
    <dbReference type="InParanoid" id="E3KWZ6"/>
    <dbReference type="OrthoDB" id="1721884at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051603">
        <property type="term" value="P:proteolysis involved in protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000138">
        <property type="entry name" value="ATP-dependent Clp protease ATP-binding subunit ClpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002836">
        <property type="entry name" value="ATP-dependent Clp protease ATP-binding subunit ClpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050052">
        <property type="entry name" value="ATP-dep_Clp_protease_ClpX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019489">
        <property type="entry name" value="Clp_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48102:SF7">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE ATP-BINDING SUBUNIT CLPX-LIKE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48102">
        <property type="entry name" value="ATP-DEPENDENT CLP PROTEASE ATP-BINDING SUBUNIT CLPX-LIKE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07724">
        <property type="entry name" value="AAA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10431">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01086">
        <property type="entry name" value="ClpB_D2-small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="4">
        <location>
            <begin position="355"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="domain" description="Clp ATPase C-terminal" evidence="5">
        <location>
            <begin position="598"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="143"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="211"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="293"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="469"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="720"/>
            <end position="741"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="143"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="241"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="300"/>
            <end position="313"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="321"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="472"/>
            <end position="493"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="720"/>
            <end position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM01086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP88889.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="741" mass="81038" checksum="6E35BF6FAF2CA9A4" modified="2012-04-18" version="2">MLKLGKRTPHLIKLKQAVVPLHGNFSNTSSFVHRSCLKFVRTSAPMCLQNFPNSGSTSLQQVRMYRLNQDHEPTTSGLSSLPSPRDIHRELDNYVIAQSDAKKILSVAVFNHYIRVNALLEVEEKRKRQVKLEADSKLAANETSQTRVNLTNTDVEQPHSSESSQNINRIPPTALRPHPPNGLAHNLIMSKAVEASLRLEREELENRIRQMTSRQMKKKHPSANQKDQGAPDDNEVLPFTPSYSSTLYQRNATSPPNAQGREWENSPIGIPNNDQVILQQLQDLNKPKIGQLADEKKSGRLQSADPTDFPTQSESRKTSIHRMNSTQSTPNESTSFNESGSETIKSQEVLDAVLNKSNILLLGPTGSGKSLLARTLARQLNVPYSESCATTFTQAGYVGEDVESAVVRLYQEANNDATKASRGIIFIDEVDKIARKSGDGYVKDVSGEGVQQAMLRMLEGTMVTVADKGSAADTSSASGRHQDPASSSGKSTQPIQVDTSNILFILAGAFVGLEKIINKRISKTSIGFSASLINQPGSDDGCDLPPVGLRSEGEGSGNTAHGRLPDALEQVEPADLNAFGLIPEFIGRIPVLASLKALTENDLLRVLTEPKNCLLKQYEQLFKINGVAIKFTLPAQRAIARLANEKKTGARGLRQIMEKILLDSMYTAPQSSIRYILIDESVVLGQTPAKFYSRGQEGLMEQDIEFEESSKGLSSALDAALSTKDSSDSSLSPTKRRKATG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>E3KXM7</accession>
    <name>E3KXM7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutamate-5-semialdehyde dehydrogenase</fullName>
            <ecNumber evidence="2">1.2.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Glutamate-5-semialdehyde dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Glutamyl-gamma-semialdehyde dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_14930</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-glutamate 5-semialdehyde + phosphate + NADP(+) = L-glutamyl 5-phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19541"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58066"/>
            <dbReference type="ChEBI" id="CHEBI:58274"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.41"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the gamma-glutamyl phosphate reductase family.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.41" evidence="2"/>
    <dbReference type="EMBL" id="DS178319">
        <property type="protein sequence ID" value="EFP89089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333508.1">
        <property type="nucleotide sequence ID" value="XM_003333460.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KXM7"/>
    <dbReference type="FunCoup" id="E3KXM7">
        <property type="interactions" value="277"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KXM7"/>
    <dbReference type="GeneID" id="10546558"/>
    <dbReference type="KEGG" id="pgr:PGTG_14930"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14930"/>
    <dbReference type="eggNOG" id="KOG4165">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030231_0_1_1"/>
    <dbReference type="InParanoid" id="E3KXM7"/>
    <dbReference type="OMA" id="KTQRYGT"/>
    <dbReference type="OrthoDB" id="1934954at2759"/>
    <dbReference type="UniPathway" id="UPA00098">
        <property type="reaction ID" value="UER00360"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004350">
        <property type="term" value="F:glutamate-5-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd07079">
        <property type="entry name" value="ALDH_F18-19_ProA-GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.309.10:FF:000006">
        <property type="entry name" value="Gamma-glutamyl phosphate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00412">
        <property type="entry name" value="ProA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020593">
        <property type="entry name" value="G-glutamylP_reductase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012134">
        <property type="entry name" value="Glu-5-SA_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000965">
        <property type="entry name" value="GPR_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001221">
        <property type="entry name" value="PRK00197.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00407">
        <property type="entry name" value="proA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11063:SF8">
        <property type="entry name" value="DELTA-1-PYRROLINE-5-CARBOXYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11063">
        <property type="entry name" value="GLUTAMATE SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000151">
        <property type="entry name" value="GPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01223">
        <property type="entry name" value="PROA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0641">Proline biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="12">
        <location>
            <begin position="5"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059423"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075718"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00077451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP89089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="466" mass="51321" checksum="05A9EA9A3B35A8F9" modified="2011-01-11" version="1">MKKTVINEVASSAKKAFDELQSIDDRNKKTEGLERLKRALVERKEEILRANDLDVQAAEGLVKEGKLSESIVNRLNLRSSSDKFDSIVDGIEAVIKLEDPTGKIQSVTKLDEGLELYKVSCPIGVLLIIFEARPEVLVNIAALAVKSGNAAILKGGKESNKTQEVLTTIINEALSSCLPANLIQTVSSRSDINELLEQNQFIDLVIPRGSKELVQFIQNHTKIPVLGHADGLCNIYVDQDADPQKVIRCVLDAKTTYPAACNSVETLLVHRSWLGDSFTELVLSLLNEKVTLKLDQASLDVLKSSGKVTNHLLFWSNCSLAREEDFKTEWLSLTLSIKTVDDLTAAIEHINQYSSHHTDSILTESSSHGSRFCRAVNSASVYVNCSTRFADGFRYGFGTEIGISTSKIHSRGPVGLDGLVIYKWMMFGSGDRAHITADYGQGKLRQFLHSNVPVDQPGLFNPFSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3KYZ4</accession>
    <name>E3KYZ4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mitochondrial import inner membrane translocase subunit TIM54</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_15668</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion inner membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TIM54 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178323">
        <property type="protein sequence ID" value="EFP89519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333938.1">
        <property type="nucleotide sequence ID" value="XM_003333890.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KYZ4"/>
    <dbReference type="STRING" id="418459.E3KYZ4"/>
    <dbReference type="GeneID" id="10547487"/>
    <dbReference type="KEGG" id="pgr:PGTG_15668"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15668"/>
    <dbReference type="eggNOG" id="ENOG502QPMQ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033744_0_0_1"/>
    <dbReference type="InParanoid" id="E3KYZ4"/>
    <dbReference type="OMA" id="RNWMIFF"/>
    <dbReference type="OrthoDB" id="5598305at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021056">
        <property type="entry name" value="Mt_import_IM_translocase_Tim54"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11711">
        <property type="entry name" value="Tim54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="7" id="KW-0653">Protein transport</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0811">Translocation</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="277"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="319"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="471"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="319"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="12">
        <location>
            <begin position="479"/>
            <end position="496"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP89519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="549" mass="62040" checksum="6129DB5B59528D6A" modified="2011-01-11" version="1">MPDTSSNQHTHSTAPKPASSINPAFRYLGIPASWLGTDRPRIRLPSAKTSGFLLSCGSLISLYVYDRHQCKKIKENYLNQIQHLSQSLVSDPTDDGFLNNDDAHWMPRRVTVYGARVPEDVDVDRGTQWFKKYVKPILIAAAIDYTIHNGTTPGALGRKISADIRDQRIIAAAQEKDGHRVPALKPGMPGYREWHAEQHRLTGGTLILGRATLKEYLWGLKHGYEQHIPLDELDDDERFSRQLEEAGIFELDECPGEFSSNEDLTMPEGLRSVQEQLKQTDGHDEISFSSLSVDDKSSPSLEKPSNFFNFFSQSKHTPSLSYSNSSSNQSTTKTLAPTPIPPQAPLLLVPFDHPIGIRWWPLKIYRFFNRRQQVETGAQVAMKLVRSQTRPIEPPKQTAGLTDSPKDCSTTGIAAADVEDTNFEIQLTGSQDLDFLAPEADQHLRRSYKKVGKRVIEERDLFRKELKDRLRKSREETNDQVGETGQSEPLNESQLRQEAFQKEKKWSDQLHGWSIVRQGSGVSWSPEMSSALKIYDSKHQDGMIMTSEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZS2</accession>
    <name>E3KZS2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_15759</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178326">
        <property type="protein sequence ID" value="EFP89803.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334222.2">
        <property type="nucleotide sequence ID" value="XM_003334174.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZS2"/>
    <dbReference type="STRING" id="418459.E3KZS2"/>
    <dbReference type="GeneID" id="10545454"/>
    <dbReference type="KEGG" id="pgr:PGTG_15759"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15759"/>
    <dbReference type="HOGENOM" id="CLU_000991_4_2_1"/>
    <dbReference type="InParanoid" id="E3KZS2"/>
    <dbReference type="OrthoDB" id="1678912at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141052">
        <property type="term" value="F:histone H3 demethylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16100">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15543">
        <property type="entry name" value="PHD_RSF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.60:FF:000016">
        <property type="entry name" value="Putative Lysine-specific demethylase 5B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.60">
        <property type="entry name" value="ARID DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001606">
        <property type="entry name" value="ARID_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036431">
        <property type="entry name" value="ARID_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003349">
        <property type="entry name" value="JmjN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019786">
        <property type="entry name" value="Zinc_finger_PHD-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10694:SF33">
        <property type="entry name" value="LYSINE-SPECIFIC DEMETHYLASE 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01388">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02375">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01014">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00501">
        <property type="entry name" value="BRIGHT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00545">
        <property type="entry name" value="JmjN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46774">
        <property type="entry name" value="ARID-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51011">
        <property type="entry name" value="ARID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51183">
        <property type="entry name" value="JMJN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01359">
        <property type="entry name" value="ZF_PHD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50016">
        <property type="entry name" value="ZF_PHD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="JmjN" evidence="9">
        <location>
            <begin position="33"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="domain" description="ARID" evidence="8">
        <location>
            <begin position="98"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="domain" description="PHD-type" evidence="7">
        <location>
            <begin position="313"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="201"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="219"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="230"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="264"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="287"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP89803.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="392" mass="43984" checksum="174467FA4DB47D7C" modified="2012-04-18" version="2">MEPKFALGSIRTEVPREFPKSKQANRLFGLEHCPVFYPTIDEFKEPMKYFESIGSKLKPFGIGKIVPPVGWKPPFVLDTDQFKFKTRLQRLNSMEASARANVNFLEQLYLFHKQRGSAGLVNSTSQLQVPVIDHRPVDLWRLRKEINELGGYDTITLQRKWSFLAKTMGYNVKTSPAVSFKLKSAYAKIIAPFDEYFNRIKQSPPKPTTHLAHRDRSPADSLSPLSSSLADHDEANRVSGSKELKKHDDGLITDESVKLPANAQGPSTRKASMTSTDNPLEEKPRSSTRPIRRPSTSHPSTSHDLDTSMDHGGDICEICGSDEDDPNILLCDSCDKGYHLQCLTPPLLTVPEGNWYCDACIVSTGNEFGFEEGRDTLFLVFSDERTDSKDVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3L0C2</accession>
    <name>E3L0C2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16189</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178327">
        <property type="protein sequence ID" value="EFP89901.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334320.2">
        <property type="nucleotide sequence ID" value="XM_003334272.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L0C2"/>
    <dbReference type="GeneID" id="10545716"/>
    <dbReference type="KEGG" id="pgr:PGTG_16189"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16189"/>
    <dbReference type="HOGENOM" id="CLU_1251205_0_0_1"/>
    <dbReference type="InParanoid" id="E3L0C2"/>
    <dbReference type="OrthoDB" id="10383344at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174287" evidence="1">
        <location>
            <begin position="26"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89901.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="224" mass="25544" checksum="85D026335AAC6DD7" modified="2012-04-18" version="2">MVMCSGFPILKVIVLCLYYFQTTQCMNGAPKGKGEALLVTDEAGKPLGVATPMVHGDKSYVGSSKNVEQHLPSGDMEKYFYSVKQLRKPTPGLYKEHLDNVMVNTQSEKPGSSDAAPSNPYWATMWAKDIWEEFKQLNQDHDNLINAIEHRKVLKEKKTGSREYDYKVKRQQTQKLILTKQAKDLKFVLNSLNDYIEVSLRGWAWQNNVNPAFFSREFASVKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3L0V3</accession>
    <name>E3L0V3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Amino acid transporter transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_16456</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the amino acid/polyamine transporter 2 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178330">
        <property type="protein sequence ID" value="EFP90178.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334597.2">
        <property type="nucleotide sequence ID" value="XM_003334549.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L0V3"/>
    <dbReference type="STRING" id="418459.E3L0V3"/>
    <dbReference type="GeneID" id="10546238"/>
    <dbReference type="KEGG" id="pgr:PGTG_16456"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16456"/>
    <dbReference type="eggNOG" id="KOG1303">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009646_8_0_1"/>
    <dbReference type="InParanoid" id="E3L0V3"/>
    <dbReference type="OrthoDB" id="2499304at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015186">
        <property type="term" value="F:L-glutamine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015824">
        <property type="term" value="P:proline transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013057">
        <property type="entry name" value="AA_transpt_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950">
        <property type="entry name" value="AMINO ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22950:SF692">
        <property type="entry name" value="TRANSMEMBRANE AMINO ACID TRANSPORTER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01490">
        <property type="entry name" value="Aa_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="292"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="349"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="402"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="485"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="631"/>
            <end position="652"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="658"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="694"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="domain" description="Amino acid transporter transmembrane" evidence="10">
        <location>
            <begin position="268"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="66"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="568"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="93"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="8">
        <location>
            <begin position="103"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="190"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="210"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="244"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="576"/>
            <end position="603"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP90178.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="718" mass="77955" checksum="D3A4BF144B169E7A" modified="2012-04-18" version="2">MDQEAASSHPAEASPPESPEQPHAGYRSVIASSINLVQSYRRSQLFIGDNLPNSFSESSFNANYLSSRQHRGVHDGAESDNDDDGRSYATSFSDRDGEIERAEGDEEYSDDEDTDRDLPSSFLPPNAFGSRRLPASRCSRASQASFPRQWTRQTSGSARSRLPNQEGDLPGGPHSPRSTQFNRTLERTPTAESSQLITPQLSFPRYGSLNGESSGRRFSTARSSFRHRERPDSEAGSAAESQDPRQPTPSHCSSTSRADFRHPPALGTSTFGQTLFNAFNILCGVGLLSEPLAFSAMGWVGGVLLFIFCGLSTNYTAKILARLMAEDRFLLTYNDICYKAFGRSMQYPIAGLFCLELFALSVALMVIFGDSLATIFPDISADSFKILAFCLVLPTVFMPLPLLSYTSLIGLVSSLTLVGVVVFDGLVKEEAPGSIFHPAKTSLSPSHRWGLSAGLMMSGFSGHSVMPSLAREMRNPQDFNRMVDYAYVAAGSMYLIVGLIGYLMFGDDVSQEITQDLLRTPGFPTAINHFGIWMVGINPVAKFALCTRPLNVTIEHLLNLTSMDDMSDPHAPAIQKRSTPVPTSQALPKNAVSSHPNQLTPRSTHSDLRGSYHEAASGSAGKNKPAMFTKALGRTISRTVVTSLVVIVSILLPNFERVMGFLGAFAAFVICTILPVSAEMIMTRGQGRSPTTKIINIVLLVVSVVMAAIGTTYSFLPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3L1P4</accession>
    <name>E3L1P4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178333">
        <property type="protein sequence ID" value="EFP90469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334888.1">
        <property type="nucleotide sequence ID" value="XM_003334840.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L1P4"/>
    <dbReference type="STRING" id="418459.E3L1P4"/>
    <dbReference type="GeneID" id="10530365"/>
    <dbReference type="KEGG" id="pgr:PGTG_16056"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16056"/>
    <dbReference type="HOGENOM" id="CLU_1143026_0_0_1"/>
    <dbReference type="InParanoid" id="E3L1P4"/>
    <dbReference type="OrthoDB" id="2506909at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="133"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="194"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="243" mass="28052" checksum="4AC308F1A61E4CBA" modified="2011-01-11" version="1">MRRQIGSHAATLFCSFCLLHKKDLEETDQTKFPVRSNEQHLLHAKEWLALDSYKGRQSFMKKYSARWSVLNELPYWRPVDHCSIELMHALILGDLKDHSLRFLYLPSAAKQLQATQEKEEEWQMDDHFTALPFTNLFPAEPKKNPGKRKRTEEPNDEPTSLKPAKRGRNTQPGPSLQGRQSKYQPNPVSESEDSSNQSSETTQTSHSYNLRARKQAIYNICNESADTEHSEDDRNSDCTLFIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3L2F7</accession>
    <name>E3L2F7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16770</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178336">
        <property type="protein sequence ID" value="EFP90744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335163.1">
        <property type="nucleotide sequence ID" value="XM_003335115.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L2F7"/>
    <dbReference type="FunCoup" id="E3L2F7">
        <property type="interactions" value="409"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L2F7"/>
    <dbReference type="GeneID" id="10530649"/>
    <dbReference type="KEGG" id="pgr:PGTG_16770"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16770"/>
    <dbReference type="eggNOG" id="KOG2830">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041824_2_0_1"/>
    <dbReference type="InParanoid" id="E3L2F7"/>
    <dbReference type="OMA" id="EYELCEA"/>
    <dbReference type="OrthoDB" id="10261753at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051721">
        <property type="term" value="F:protein phosphatase 2A binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035303">
        <property type="term" value="P:regulation of dephosphorylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009966">
        <property type="term" value="P:regulation of signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.540">
        <property type="entry name" value="TAP42-like family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038511">
        <property type="entry name" value="TAP42/TAP46-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007304">
        <property type="entry name" value="TAP46-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10933">
        <property type="entry name" value="IMMUNOGLOBULIN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10933:SF9">
        <property type="entry name" value="IMMUNOGLOBULIN-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04177">
        <property type="entry name" value="TAP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="166"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="179"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="337"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="390" mass="44206" checksum="ED1DAED2BF132A10" modified="2011-01-11" version="1">MSQENDITLGQLLDRATSAYNHVIEQPTPNEETVQSKILAALADLSLSSSLINRLAILSPNETLEDINTNDLKCLSVEVLRGLLVVLLKTKGGGQRKKNLEQAKDHFKSFSRQIEAYEIIPREQIERFKGPLSAIQSASMRRESKINQYKLEKQLKQQLDELRERKRAAQRSRLYSSGRSKEDYNSRVERANGSDDDDDENESESRSTHLTWLKFLYLKAHQELDSIEAELEILGQAAQMNDLPSKKVQREEDDLSWRVERLSTSTDGPLVSPSGKVLRPFTILPSRSSASSASTNRIKLRAEVFRPDWTLPTMTIDDYLDEQRAMGNFLSGGGPDQASQETPSQRAQIDAEEDNLKGELKAEELRKKAVEWNEFTDSHRKGEGNMMNRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>E3L2T9</accession>
    <name>E3L2T9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Mitochondrial-processing peptidase subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_17063</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Substrate recognition and binding subunit of the essential mitochondrial processing protease (MPP), which cleaves the mitochondrial sequence off newly imported precursors proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase M16 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178338">
        <property type="protein sequence ID" value="EFP90864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335283.1">
        <property type="nucleotide sequence ID" value="XM_003335235.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L2T9"/>
    <dbReference type="FunCoup" id="E3L2T9">
        <property type="interactions" value="672"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L2T9"/>
    <dbReference type="GeneID" id="10530770"/>
    <dbReference type="KEGG" id="pgr:PGTG_17063"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17063"/>
    <dbReference type="eggNOG" id="KOG2067">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009902_5_2_1"/>
    <dbReference type="InParanoid" id="E3L2T9"/>
    <dbReference type="OMA" id="LKYHHSP"/>
    <dbReference type="OrthoDB" id="2504195at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006627">
        <property type="term" value="P:protein processing involved in protein targeting to mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.830.10">
        <property type="entry name" value="Metalloenzyme, LuxS/M16 peptidase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011249">
        <property type="entry name" value="Metalloenz_LuxS/M16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050361">
        <property type="entry name" value="MPP/UQCRC_Complex"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011765">
        <property type="entry name" value="Pept_M16_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007863">
        <property type="entry name" value="Peptidase_M16_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851">
        <property type="entry name" value="METALLOPROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11851:SF49">
        <property type="entry name" value="MITOCHONDRIAL-PROCESSING PEPTIDASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00675">
        <property type="entry name" value="Peptidase_M16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05193">
        <property type="entry name" value="Peptidase_M16_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63411">
        <property type="entry name" value="LuxS/MPP-like metallohydrolase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M16 N-terminal" evidence="4">
        <location>
            <begin position="64"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M16 C-terminal" evidence="5">
        <location>
            <begin position="216"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="26"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="26"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP90864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="576" mass="63216" checksum="32340E22FB74F906" modified="2011-01-11" version="1">MATRRWTTTTSRAFKPFTSLNRTLTHSAQKKSTTTLPIRRSSYSTTHHGSPEETRISTLSNGLRVTTESTPGHFIGAGVYVDAGSRYESAYLRGSTHLTDRMAFKSTQNRTTEEISLEIEQLGGSFFASSGRDTVLYQATSYPDSLPSVLSVLSDTALNPLLKDSELAAEQEAAEWEVNEINKKPEYMIPEILHETAFPNNTLGLPLICPKDRIHSITPEVLWSYRSMFFKPERIVVAGVGVDHDYFLSHVEQYFGNFKSVKPTVPLNTNFGPAISSATANAATVIGSSFTNPSAGSKSLLSKAFATTTNVSPVDLTTGQSLQSFEELISAKPVYRGGEVRIPGKTESNLAHIYIGFEAPSVHDDDLYAIACTHIMLGGGSSFSAGGPGKGMYSRLYTNVLNPHPEVDFCQAFHHTYADAGLFGIAMAVAPEFASHVPQIIASQLDLISRDQSRGGITEAQLRRAKNQLRSTMMYGLESRLLQVEDLGRQVQTAGRKKPWADVWERIESLTIKDIHRAITKIIRPGSSRQSSSDGKMFSGEPTIVATGMIDRLGDIKELFSRYGIGPALPRSNRFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>E3L2Y4</accession>
    <name>E3L2Y4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Inorganic phosphate transporter pho88</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178339">
        <property type="protein sequence ID" value="EFP90909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335328.1">
        <property type="nucleotide sequence ID" value="XM_003335280.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L2Y4"/>
    <dbReference type="FunCoup" id="E3L2Y4">
        <property type="interactions" value="94"/>
    </dbReference>
    <dbReference type="GeneID" id="10528319"/>
    <dbReference type="KEGG" id="pgr:PGTG_17181"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17181"/>
    <dbReference type="eggNOG" id="KOG4554">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_099163_0_0_1"/>
    <dbReference type="InParanoid" id="E3L2Y4"/>
    <dbReference type="OMA" id="NMDYDKG"/>
    <dbReference type="OrthoDB" id="18139at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045047">
        <property type="term" value="P:protein targeting to ER"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012098">
        <property type="entry name" value="SND3_fun"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28112">
        <property type="entry name" value="SRP-INDEPENDENT TARGETING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28112:SF1">
        <property type="entry name" value="SRP-INDEPENDENT TARGETING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10032">
        <property type="entry name" value="Pho88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF008756">
        <property type="entry name" value="P_tr_PHO88"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="170" mass="19167" checksum="66017A57F6358D84" modified="2011-01-11" version="1">MQVARKIPFGDPQVLFGMRMAYVSSQVICLSIYYWLSHKIKTKNDTTVLKYVEPSPPMTNEPGKLVTTTNRDYDLAQSSSGLRSVLMGVLMMCFLHLYMKYDAPLFLQSIMPLKTVYDNKLVQIHLFGHPATGDYKRPFQTSGFMGAATNAKTDAKSIKEAEQASVKKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>E3L321</accession>
    <name>E3L321_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Malate dehydrogenase</fullName>
            <ecNumber evidence="3 12">1.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGTG_17218</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 12">
            <text>(S)-malate + NAD(+) = oxaloacetate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21432"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15589"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.37"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH/MDH superfamily. MDH type 1 family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="3 12"/>
    <dbReference type="EMBL" id="DS178339">
        <property type="protein sequence ID" value="EFP90946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335365.1">
        <property type="nucleotide sequence ID" value="XM_003335317.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L321"/>
    <dbReference type="SMR" id="E3L321"/>
    <dbReference type="FunCoup" id="E3L321">
        <property type="interactions" value="567"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L321"/>
    <dbReference type="GeneID" id="10528360"/>
    <dbReference type="KEGG" id="pgr:PGTG_17218"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17218"/>
    <dbReference type="eggNOG" id="KOG1494">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047181_1_0_1"/>
    <dbReference type="InParanoid" id="E3L321"/>
    <dbReference type="OMA" id="ASCAEYI"/>
    <dbReference type="OrthoDB" id="4069699at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006108">
        <property type="term" value="P:malate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01337">
        <property type="entry name" value="MDH_glyoxysomal_mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000013">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.110.10:FF:000001">
        <property type="entry name" value="Malate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001252">
        <property type="entry name" value="Malate_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010097">
        <property type="entry name" value="Malate_DH_type1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01772">
        <property type="entry name" value="MDH_euk_gproteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540">
        <property type="entry name" value="MALATE AND LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11540:SF73">
        <property type="entry name" value="MALATE DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00068">
        <property type="entry name" value="MDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 12" id="KW-0520">NAD</keyword>
    <keyword evidence="5 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="13">
        <location>
            <begin position="36"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="14">
        <location>
            <begin position="182"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="212"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="42"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="122"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="129"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="152"/>
            <end position="154"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EFP90946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="353" mass="36967" checksum="6C917EA3DB4B11A9" modified="2011-01-11" version="1">MAIAPISSIIRHNVTPTIFKSHSVARQFSSSSQNFTKVAILGAAGGIGQPLSLLMKQSSLVSELALYDVQGSPGVAADVSHVNTASTCKGYLPDGEGLEKALDGAQIVLVPAGVPRKPGMTRDDLFNKNASIAADLATAAAKVCPKAHMLIIANPVNSTVPIVGEVFKKHNVFDPKRLFGVTTLDVVRASAFLSSLAKSHPKDTNVQVIGGHSGVTIVPLLSQVAQGKSITGEAYKALVKRIQFGGDEVVEAKSGAGSATLSMAYAAAIFTESLLKALGGARGIIEPTFVKSHLYEKEGVEYFASNVELGPEGVGKILPIGSVSNEEQELINACLPELKKNIEKGVKFVQGRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="73" xmlns="https://uniprot.org/uniprot">
    <accession>E3L346</accession>
    <name>E3L346_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">peptidylprolyl isomerase</fullName>
            <ecNumber evidence="2">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_17243</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="2"/>
    <dbReference type="EMBL" id="DS178339">
        <property type="protein sequence ID" value="EFP90971.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335390.2">
        <property type="nucleotide sequence ID" value="XM_003335342.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L346"/>
    <dbReference type="STRING" id="418459.E3L346"/>
    <dbReference type="GeneID" id="10528386"/>
    <dbReference type="KEGG" id="pgr:PGTG_17243"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17243"/>
    <dbReference type="eggNOG" id="KOG0546">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012062_33_1_1"/>
    <dbReference type="InParanoid" id="E3L346"/>
    <dbReference type="OrthoDB" id="407558at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016018">
        <property type="term" value="F:cyclosporin A binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000025">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase CYP19-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020892">
        <property type="entry name" value="Cyclophilin-type_PPIase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11071:SF561">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00170">
        <property type="entry name" value="CSA_PPIASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="6">
        <location>
            <begin position="30"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="197"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="199"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="232"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="257"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="285"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP90971.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="380" mass="42962" checksum="3AC6D406475FD886" modified="2012-04-18" version="2">MVIAIGHRHCTHHIHHLAHTMSNPSNPRVFFDFSINNAPGRRVIFELFKDRVPITAENFRALCTGEKGYSTIAPETLLCYKGSPVHRIVPNFMIQGGDFIQQNGKSGESIYGGEFDDENFELDCDREGLLVMANKGPNTNQSQWFITLTPADHLTGKHVVFGRVQSGFEVVQEIGGLETDRKHRPLGGAEAVKIVHCGQLERKQKPPVEKTVDEPVKNRSDSKLDRKRHSRSSSVASSASSRRSSSSSGSSESSGAARRRRRQEKKEKKKARKSQKSSKSKKLKFKEQPVEEHPPQADDPRAQEELALIKVELEHQQAERQRQSEEELARLEEEKARKARALELLKNKPHRKEGGVIFKGRGSMRYRDSDVTFGTRAWKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3E4</accession>
    <name>E3L3E4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF202 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_16931</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178341">
        <property type="protein sequence ID" value="EFP91069.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335488.2">
        <property type="nucleotide sequence ID" value="XM_003335440.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L3E4"/>
    <dbReference type="STRING" id="418459.E3L3E4"/>
    <dbReference type="GeneID" id="10528648"/>
    <dbReference type="KEGG" id="pgr:PGTG_16931"/>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
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</entry>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
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                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
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                <person name="Ellis J.G."/>
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                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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                <person name="Martin F."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
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                <person name="Brockman W."/>
                <person name="Young S."/>
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                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
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                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
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                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
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                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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            <dbReference type="PubMed" id="21536894"/>
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            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
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    </evidence>
    <sequence length="288" mass="31995" checksum="E63F6B24131BD1A7" modified="2012-04-18" version="2">MEESQARRRRRSTSDRMGEVTAEDRSASRTSNTPDIIGDPSTRHSASIPHSPSSAVEQYKLLRSVAIGLIDQAIDLLHHVVVSDGLLSSVSELVPGSTIGKHLRHLHDHFRILFECALSGLPGHHKVLNYDTRERNVPMESSHKTILVEFERLKHRYLGDPESPLNVEPNAVEPFCEDNQDPPVLDRASSPSAHPSTSSSSSSSLSLAMSRKKGCSRPRFDHDMALDLVAITPHRVGLKTTFNRELWFACLHATHHYALIRVIVTGELKLSLPKEFGVAPSTLERSKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3L407</accession>
    <name>E3L407_PUCGT</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
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    <gene>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
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                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178344">
        <property type="protein sequence ID" value="EFP91282.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335701.2">
        <property type="nucleotide sequence ID" value="XM_003335653.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L407"/>
    <dbReference type="GeneID" id="10529030"/>
    <dbReference type="KEGG" id="pgr:PGTG_17139"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17139"/>
    <dbReference type="HOGENOM" id="CLU_031648_0_0_1"/>
    <dbReference type="InParanoid" id="E3L407"/>
    <dbReference type="OrthoDB" id="10295839at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP91282.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="616" mass="70373" checksum="0BB29C288D8F184D" modified="2012-04-18" version="2">MSRAVDAKIEAAAPLNLTLIMILITTLTRRLSYQLYIAAWLFTLALKSCELFDLNKLPTEYFDEAFPSVSAEISRGKFKEHLVQHSILSPRQTCTSIPMIRSAPLTQVPSTNSTGKSVSLPAVAQSQYPHLDNDLSDTDLPGIPAVEEKDWDELDFSHFITRGIVIEELLHTFSERFKSQVMSQDCRSRHWGLVDPHPTVSTARIDVAVEDPYKLAKVLHYDLASTQTAKHLYMLYKFLVVSLFKLHGEFLTQINIPVLHHRNQQEKLLEWLDKQIFSPGDSHPVLGIAPESDLTWESGGPSLQYYGDIQKELILYFSDDKRSDPNICASAILKPYRTEHAKEYLALRQPSGSFLEDPNRLSIKPHFLLILEYLSNMAKKLEINLLNSDDRHSKQALEHSALVTPYVSTFEKRFRTSEIEKGDVRSYYPTLQITMCFHDHKPGPKPLRMIDPAGKIYKFNDLRSRLRKLMKALDCLTIGAMSELKIRLSDKDIKIRRKSVLKFLVDSMIQPKGSLPLIGSVESPEGIAPWFSNSHGNPVLFGEIQLKIMKYLTDYLEEEDLIQIPAFVLSAWHYANPNNDETMRYDAINRPVSVSHRFSDTRLSDTDAMHRLSVYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>E3L4K3</accession>
    <name>E3L4K3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_17728</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178347">
        <property type="protein sequence ID" value="EFP91478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335897.1">
        <property type="nucleotide sequence ID" value="XM_003335849.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L4K3"/>
    <dbReference type="GeneID" id="10529479"/>
    <dbReference type="KEGG" id="pgr:PGTG_17728"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17728"/>
    <dbReference type="HOGENOM" id="CLU_2062645_0_0_1"/>
    <dbReference type="InParanoid" id="E3L4K3"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP91478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="119" mass="13052" checksum="28D4F70E71437B74" modified="2011-01-11" version="1">MPMTPSLSSACTTLIFAAKSFFRQCSRRVSEEPSLGHVGEASEIQYGAVQHGAGQLHSSLSCVEGREQGEKRSIFRHVDIIQVVISSEINPVLMVAKFCQTASFPDLVVSCTIDWYDVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3L581</accession>
    <name>E3L581_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phosphatidylserine decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_17443</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178351">
        <property type="protein sequence ID" value="EFP91706.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336125.2">
        <property type="nucleotide sequence ID" value="XM_003336077.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L581"/>
    <dbReference type="STRING" id="418459.E3L581"/>
    <dbReference type="GeneID" id="10531700"/>
    <dbReference type="KEGG" id="pgr:PGTG_17443"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17443"/>
    <dbReference type="HOGENOM" id="CLU_1587334_0_0_1"/>
    <dbReference type="InParanoid" id="E3L581"/>
    <dbReference type="OrthoDB" id="4330at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004609">
        <property type="term" value="F:phosphatidylserine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003817">
        <property type="entry name" value="PS_Dcarbxylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10067">
        <property type="entry name" value="PHOSPHATIDYLSERINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10067:SF6">
        <property type="entry name" value="PHOSPHATIDYLSERINE DECARBOXYLASE PROENZYME, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02666">
        <property type="entry name" value="PS_Dcarbxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="2" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP91706.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="168" mass="18543" checksum="7A7A9E1A75E0E563" modified="2012-04-18" version="2">MGSSENTQQGQDPTESFVASQPVSPGAGLASSVKVHTDIVRGELETLPSSGNCMFFFVVYLAPGNYHCFHSPADWKVERCCHFAGLFTPWLHLSLKNGMTIGELFSVSPWMGGELADLFVLNKRVALLGQWRHGFFSMTPVGAKNVGSILINLNQELRTNWVDKQCHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3L5I7</accession>
    <name>E3L5I7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_17812</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178352">
        <property type="protein sequence ID" value="EFP91812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336231.1">
        <property type="nucleotide sequence ID" value="XM_003336183.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L5I7"/>
    <dbReference type="GeneID" id="10531757"/>
    <dbReference type="KEGG" id="pgr:PGTG_17812"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17812"/>
    <dbReference type="HOGENOM" id="CLU_559142_0_0_1"/>
    <dbReference type="InParanoid" id="E3L5I7"/>
    <dbReference type="OrthoDB" id="2495962at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP91812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="488" mass="56660" checksum="842536BD12CA1FD8" modified="2011-01-11" version="1">MTDLPIEIPLHIFLLALLDEEDQADYPSSPAYWSCTLKTQSVLWGRGIELCRRAWNKSITLRMTCRIANDQICRQMNQSAILAGLIQTPSNPRSHARTLPCHRLKLYTRNGCLGGLELLKRHSQTLEFFFIEMAHALPGSIWWNASPPLNLAATTFPRLTTFIFRTNSAGVWLSFRNLAELMRNSPGLRHLTVFQLTGPADDEEANELLANQGQPACQLESLHIRRARWLFDDNLMFIVADSHLSLRELTWVFEKPDTDEEGNLSDDEREDDADAFYSIFAPCTEIETLRIADLRIDGLVRRRKHQRRERFNRSSPMGRVLGNLLAKLWNLENLELCGTINIQHLYKRNVDVLPFDLRSLFIDRYHEYRLDKLIHQLQREEGPLGQLESLAIDEQVELDVQPFLWETLLTVCQSRGIQLRIHRDDLSRNDDPEDLSVRLQRLAIDTIPVNQTRYRFACLEGFLFQNRHNGERYEFLYDKTPGLSCFLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>E3L5V5</accession>
    <name>E3L5V5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_17957</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178354">
        <property type="protein sequence ID" value="EFP91930.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336349.2">
        <property type="nucleotide sequence ID" value="XM_003336301.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L5V5"/>
    <dbReference type="STRING" id="418459.E3L5V5"/>
    <dbReference type="GeneID" id="10531879"/>
    <dbReference type="KEGG" id="pgr:PGTG_17957"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17957"/>
    <dbReference type="HOGENOM" id="CLU_018552_2_0_1"/>
    <dbReference type="InParanoid" id="E3L5V5"/>
    <dbReference type="OrthoDB" id="2641813at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF85">
        <property type="entry name" value="GH03217P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="8">
        <location>
            <begin position="244"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP91930.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="340" mass="39315" checksum="4FD5FA0CE3574A68" modified="2012-04-18" version="2">MVHVRLFSRFAELEIVSKSTTTLVMRQYSERQCLIREILMILLRLEYEETDLMVKRAVGIRPLQLMAELAFPHSQILQTTYNALFSDETEYQDLLQYMLSNRYLEPRGAPKSQGEFDIERLFDMSDNDFRQAARTTKHGFIKVLDTIFDNEVFHCGGHRPQLPIPHQLALTLERLGSNGNGASVGQFSRNLQVARGTVVKVTRRVIEALISMGRVYVQWPDKDRRAEISEVMRMEGFSGCVGFVDGTTIPIFQRPGFDGETFFDRKKRYFMNAQIVCDCDRFITSFISGWPGSCGDSKVYQRMQLHQNPSQFFDQGYEPRAYLSVIRLQQRPDEVVNAYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3L6A9</accession>
    <name>E3L6A9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18174</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178357">
        <property type="protein sequence ID" value="EFP92084.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336503.2">
        <property type="nucleotide sequence ID" value="XM_003336455.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L6A9"/>
    <dbReference type="GeneID" id="10532042"/>
    <dbReference type="KEGG" id="pgr:PGTG_18174"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18174"/>
    <dbReference type="HOGENOM" id="CLU_2441954_0_0_1"/>
    <dbReference type="InParanoid" id="E3L6A9"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92084.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="90" mass="10270" checksum="A127FFD6C8CEBA7A" modified="2012-04-18" version="2">MKPGNQTSPVPYPKVIAHTKPFEAEDLQNGLGKTDKEGIVTQNSMQKSGKSKKRRKKKIPVLKQASEENYLHPLKKDVIEIQKRLYDLQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8F5</accession>
    <name>E3L8F5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pectinesterase inhibitor domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18894</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178374">
        <property type="protein sequence ID" value="EFP92830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337249.1">
        <property type="nucleotide sequence ID" value="XM_003337201.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L8F5"/>
    <dbReference type="GeneID" id="10542544"/>
    <dbReference type="KEGG" id="pgr:PGTG_18894"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18894"/>
    <dbReference type="HOGENOM" id="CLU_1468921_0_0_1"/>
    <dbReference type="InParanoid" id="E3L8F5"/>
    <dbReference type="OrthoDB" id="2497752at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174472" description="Pectinesterase inhibitor domain-containing protein" evidence="1">
        <location>
            <begin position="22"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="189" mass="20634" checksum="22D2276F47FD8E82" modified="2011-01-11" version="1">MFIRLLIALSLASLLVQGVFPNPNGDSPLSTNLRPRSVNSAHSATALKSEFSDIHGEIRNAYGPLERACKENDLKSVVATFAGFQKSFQGLANSCSKTYNQNRKSPSKMASGFVKILVEFQPLLKTLKAHPSMLKGCSNTFRGSSTSINAMVSFLKAGKADLKSEVRNSGKDLDMELFAQCGFRLNTFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3L966</accession>
    <name>E3L966_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19371</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178381">
        <property type="protein sequence ID" value="EFP93091.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337510.2">
        <property type="nucleotide sequence ID" value="XM_003337462.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L966"/>
    <dbReference type="GeneID" id="10543156"/>
    <dbReference type="KEGG" id="pgr:PGTG_19371"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19371"/>
    <dbReference type="HOGENOM" id="CLU_769723_0_0_1"/>
    <dbReference type="InParanoid" id="E3L966"/>
    <dbReference type="OrthoDB" id="10300245at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="77"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93091.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="298" mass="33157" checksum="7AAFE0B6B49E5936" modified="2012-04-18" version="2">MSAGTTTPSGLVPRFRARVTQSPGCSSLAIFNEAQHNRSSSGQPIPDPGTEPIKATAKLMIKAKLMINPNLEEEARTIGTPAQPQPLSEDKSDLASNPDHPHVNQTLADEDTDSEGDRPSDLPQNVPNRPIAIDRAPQGNQMIVALQSTLALSEADLQPIHNWPTLNQQQKTFAIAMYLQSINHQIDQLAAQAGPNAGPMGHVWHQDFRAFIHTHLWEKLLQSDLMSYGRTHTVKNCSLIPKTPLILVKEEIDEMDDNWKRAYLPAGYLDDRQDDITLLNNLIRELLKYEKSTFAKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>E3L9M5</accession>
    <name>E3L9M5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_19196</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178386">
        <property type="protein sequence ID" value="EFP93250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337669.1">
        <property type="nucleotide sequence ID" value="XM_003337621.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L9M5"/>
    <dbReference type="GeneID" id="10543482"/>
    <dbReference type="KEGG" id="pgr:PGTG_19196"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19196"/>
    <dbReference type="HOGENOM" id="CLU_2147081_0_0_1"/>
    <dbReference type="InParanoid" id="E3L9M5"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP93250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="112" mass="12503" checksum="16BAEFAEF33CC15F" modified="2011-01-11" version="1">MTAWRRMQIFGPESFSIPERKGLKSFWMGLSSFLHPMLPVKSCDNGLGYSIDYGPLKTKLFNWSILLSASGEENTDSILSQSSKWQKVPFAHPTSLDAPTLPDIYLHRVGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3LA77</accession>
    <name>E3LA77_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_19239</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178393">
        <property type="protein sequence ID" value="EFP93436.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003337807.2"/>
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    <dbReference type="AlphaFoldDB" id="E3LA77"/>
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    <dbReference type="KEGG" id="pgr:PGTG_19239"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19239"/>
    <dbReference type="HOGENOM" id="CLU_1245899_0_0_1"/>
    <dbReference type="InParanoid" id="E3LA77"/>
    <dbReference type="OrthoDB" id="2506087at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP93436.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="222" mass="25434" checksum="E75701B00CBF4E3D" modified="2012-04-18" version="2">MEKTVENSKKLWTYAKEPLMNLEKLNKKSSDLAVRDQINLTFCKQLFDFQEKKLNILQAGEELPPDMDQDVPQVLVDMEKNEYKRMFSPYLELKGFDTIKDTPVEILHVFLLGPVKYLFGDFMKGLDGDQKKELLALWNSFNTNSLNIPSIRATSMVQYSSSLIEILLNGLIKPNSICYFTSQNLSCALVQPVFLPQKNLKAIMVSLEMLQFTPTAKVLVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3LAM6</accession>
    <name>E3LAM6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">BZIP domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_19654</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178399">
        <property type="protein sequence ID" value="EFP93601.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338020.2">
        <property type="nucleotide sequence ID" value="XM_003337972.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LAM6"/>
    <dbReference type="STRING" id="418459.E3LAM6"/>
    <dbReference type="GeneID" id="10534808"/>
    <dbReference type="KEGG" id="pgr:PGTG_19654"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19654"/>
    <dbReference type="eggNOG" id="ENOG502RPD7">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_050264_0_0_1"/>
    <dbReference type="InParanoid" id="E3LAM6"/>
    <dbReference type="OrthoDB" id="2501196at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090575">
        <property type="term" value="C:RNA polymerase II transcription regulator complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14688">
        <property type="entry name" value="bZIP_YAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050936">
        <property type="entry name" value="AP-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004827">
        <property type="entry name" value="bZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046347">
        <property type="entry name" value="bZIP_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621:SF6">
        <property type="entry name" value="AP-1-LIKE TRANSCRIPTION FACTOR YAP1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40621">
        <property type="entry name" value="TRANSCRIPTION FACTOR KAPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00170">
        <property type="entry name" value="bZIP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00338">
        <property type="entry name" value="BRLZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57959">
        <property type="entry name" value="Leucine zipper domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50217">
        <property type="entry name" value="BZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00036">
        <property type="entry name" value="BZIP_BASIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BZIP" evidence="5">
        <location>
            <begin position="202"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="154"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="276"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="328"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="405"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="203"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="281"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="294"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="328"/>
            <end position="338"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP93601.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="443" mass="48277" checksum="40318353B32B37B0" modified="2012-04-18" version="2">MLVLLLCHSATPTRPREITEEVQRVSETSLDDIKVTALLQLASLGDEWISHNFLSLQSWLQAHYSPPIVRSSGTTQKRLVLSEFQRASGLLDFISLSSFGSLRMSPSDVPDPISPTSASAGLPSDPAYSTLSACLNGPLNRKTVSSGTVISLADLRSPSSDLPPTPKTPDFGGENQTSHRADGTYVLPVHPLSDHIKNDVNRRKEQNRNAQRAFRERKEKRLQDLQGRIETLVAKQQPLAEENRCLKQLVHQLQAENRDLNAYKTAFNLIASDSLPNSASPLPPEMQSQPPPTINTSNGYPESTPLLQFSSLRHTAMDRNNLDTCSQASIASQSSGTDSPFPHPTPGGTSQPMLLSVEYPDMTSIMPPLAGEGHGSDKMGSVKMCDFSNTTYLEQAQPQPKNSYIFGTSSDVSGSLPASEAPSGSTIHELQNWYNEINFPGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3LBG0</accession>
    <name>E3LBG0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_19898</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178415">
        <property type="protein sequence ID" value="EFP93885.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338304.2">
        <property type="nucleotide sequence ID" value="XM_003338256.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LBG0"/>
    <dbReference type="GeneID" id="10535415"/>
    <dbReference type="KEGG" id="pgr:PGTG_19898"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19898"/>
    <dbReference type="HOGENOM" id="CLU_020173_0_0_1"/>
    <dbReference type="InParanoid" id="E3LBG0"/>
    <dbReference type="OrthoDB" id="2506388at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="492"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="303"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="26"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="38"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="492"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="537"/>
            <end position="576"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP93885.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="597" mass="67186" checksum="5108E50892E07377" modified="2012-04-18" version="2">MDPLHNAWATSSSTPVPPTVRRTKKNGSPTDTESQAEVIEKLAREIESLKSNLNSQKSTSKSKGKKSTVKVTNPTANDVSTSSSSASASKKKAPVPTTPNRNQRATSAPPKFMRPPKPRKSISETPVAKPSPKRHPQQIQTGDFPAAFGPTKILWGLLRQDSVPKPPELSKLEEFYRRFRRAEQVEEAVRSGSANRDVGQVSCLKDAQGGRIKFGKSVIHLGSNFVRYAQGIMAQLGLSVWCPNLDEDSGSLYNAAHRIAALTTFTELAATPAYAYLKVDPAMAQNMTLLIPAYNHFVHYLQAEKYKKEIKQKGKVAQEASNKKHNKNRERLRDERRNFAILNKFPQRYRDILEPIAAHSDDEEVEGKGFYKIKTLPYRSNNANRFFRRLDVVMKKAAEQDPLRKSSRRRVRRLPKEPQESLYKAAPKGLPIDFYHPKWFNELVPAQQHSIPDRTRIAFLPNANESLLPKSEKNLDEKLKDSTFTRKYWEVVEESSEEEENAAEDQVNGQGDEGEGIDLTQPSEGSDSDDEYHVEGDAGELYDEEGDDESFVDNDEQEDGGEDEDEDDDEEYDEAQDGVGLDVDHPMADIPEGEEDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3LBX8</accession>
    <name>E3LBX8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20039</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178433">
        <property type="protein sequence ID" value="EFP94053.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338472.2">
        <property type="nucleotide sequence ID" value="XM_003338424.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LBX8"/>
    <dbReference type="GeneID" id="10535751"/>
    <dbReference type="KEGG" id="pgr:PGTG_20039"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20039"/>
    <dbReference type="HOGENOM" id="CLU_035345_0_0_1"/>
    <dbReference type="InParanoid" id="E3LBX8"/>
    <dbReference type="OrthoDB" id="2514177at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="352"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="402"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="434"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="435"/>
            <end position="458"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP94053.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="496" mass="55922" checksum="A4DC192FFB7D2967" modified="2012-04-18" version="2">MASDIRNTHSPPQTNNPWHNEVNRHRPVSRNSPAAFAINAKNGADPQRHPSRSRSRNAPHNRTNDRRQDEDKDGDVPIYTANKVNRFLAEALVQSLYQGKTPGQIVSNVVSRMSSADKLQPNGSNFAVWELMMRERALEIFSVPMCSMHWSILRHPNCYEMFTYLNSRFFTVTRAEQMMCWDKLINFKLSDFLSSAEAVTKLYKTLDDFVSFGLDLNRETIGGLALQSSIEAGSELRREVDCRVEQDLGGTSKTLSKKAVTPDQILKHIDIVKRQLDLGSSRSSTFSASAPQAHQATADTHSDQAREFYDPQTWPNTPEQVEGMAISGPQCWNCRSPDHLLSKFRLPRRIDFDRPPSSSNLWRRQPQHPVGNRSGHAPVMAPGNFQAWYPIVNPPGYQHCGYQSAQSPYTPPPTSLPGQQPAKRADLYRPDYRQRTPQHQPKSQSQASANAVDASNPSEEPDHHQSQPSQDCYHPPSPIQESTSRMIEIGVFDEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3LC06</accession>
    <name>E3LC06_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_19984</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178437">
        <property type="protein sequence ID" value="EFP94081.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338500.2">
        <property type="nucleotide sequence ID" value="XM_003338452.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LC06"/>
    <dbReference type="STRING" id="418459.E3LC06"/>
    <dbReference type="GeneID" id="10535777"/>
    <dbReference type="KEGG" id="pgr:PGTG_19984"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19984"/>
    <dbReference type="HOGENOM" id="CLU_012390_7_0_1"/>
    <dbReference type="InParanoid" id="E3LC06"/>
    <dbReference type="OrthoDB" id="2503752at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023:SF4">
        <property type="entry name" value="GLYCINE-RICH PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="2">
        <location>
            <begin position="200"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="217"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="350"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="36"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="222"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="238"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="350"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="394"/>
            <end position="416"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP94081.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="416" mass="46821" checksum="0F834F18DDAF434C" modified="2012-04-18" version="2">MADTDPKSSTNTPTPTPSSPKKKKKKKNRKKKSTPPKETTTDTPKIKQPTDIATPQPADKSIIDIDPTPKAANDPAPKKGNPRWSIEEDKKLCVAWLNTSRDAIVGTGQKASTFWERIHATLSDLISEYNDEKKSAKYFKPLPLRPVGAVECRWALILKSVNKFSGCYSNAERRLKSGKTRDDILTEAKELYKASFGSAFSLDHCWGILKDSPKWQATQTENEARGKKADQSRTTSVASDGPASTPAASSPAIIDVDNDNSEISRSVLGNVRVEGQKAAKRKRADKCSIKKIVGMQKELVQISRDRLSSMKAAMQSTSDNAIMSQDLSMMDDESRAYYLKKKRAIIAREEQEEKEKEEKEKKEKEENEKKARQEKEEKERQEEAEKKRKQKEAEELEYESDDGEDSEGDEETEENE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3NXJ7</accession>
    <name>E3NXJ7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20244</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS989945">
        <property type="protein sequence ID" value="EFP94296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338715.1">
        <property type="nucleotide sequence ID" value="XM_003338667.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NXJ7"/>
    <dbReference type="GeneID" id="10527422"/>
    <dbReference type="KEGG" id="pgr:PGTG_20244"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20244"/>
    <dbReference type="HOGENOM" id="CLU_1918104_0_0_1"/>
    <dbReference type="InParanoid" id="E3NXJ7"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP94296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="132" mass="14624" checksum="A3D943EDA157CBD6" modified="2011-01-11" version="1">MSQIVSPVQTSELVSSNNRDRVPKRPGTTPTTSTRFISNSSPPPLRSLLIILFITHITNSFDCSRLWTCLIKNNPLGLENLYRFNLKQSITSDLTPSKRPSMTYASAVSGIKQPLDCPSDRLKGDLNPFATT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="79" xmlns="https://uniprot.org/uniprot">
    <accession>H6QP11</accession>
    <name>H6QP11_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Ubiquitin-conjugating enzyme E2 2</fullName>
            <ecNumber evidence="3">2.3.2.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">E2 ubiquitin-conjugating enzyme 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PGTG_20712</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="22" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="19">Belongs to the ubiquitin-conjugating enzyme family.</text>
    </comment>
    <dbReference type="EC" id="2.3.2.23" evidence="3"/>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EHS63125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890680.1">
        <property type="nucleotide sequence ID" value="XM_003890631.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QP11"/>
    <dbReference type="FunCoup" id="H6QP11">
        <property type="interactions" value="454"/>
    </dbReference>
    <dbReference type="STRING" id="418459.H6QP11"/>
    <dbReference type="GeneID" id="13541457"/>
    <dbReference type="KEGG" id="pgr:PGTG_20712"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20712"/>
    <dbReference type="eggNOG" id="KOG0419">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_030988_10_2_1"/>
    <dbReference type="InParanoid" id="H6QP11"/>
    <dbReference type="OMA" id="DHKSQYI"/>
    <dbReference type="OrthoDB" id="9984419at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000781">
        <property type="term" value="C:chromosome, telomeric region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033503">
        <property type="term" value="C:HULC complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990304">
        <property type="term" value="C:MUB1-RAD6-UBR2 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097505">
        <property type="term" value="C:Rad6-Rad18 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990305">
        <property type="term" value="C:RAD6-UBR2 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990303">
        <property type="term" value="C:UBR1-RAD6 ubiquitin ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070628">
        <property type="term" value="F:proteasome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061631">
        <property type="term" value="F:ubiquitin conjugating enzyme activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034620">
        <property type="term" value="P:cellular response to unfolded protein"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071629">
        <property type="term" value="P:cytoplasm protein quality control by the ubiquitin-proteasome system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007535">
        <property type="term" value="P:donor selection"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042275">
        <property type="term" value="P:error-free postreplication DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070987">
        <property type="term" value="P:error-free translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042276">
        <property type="term" value="P:error-prone translesion synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042138">
        <property type="term" value="P:meiotic DNA double-strand break formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031571">
        <property type="term" value="P:mitotic G1 DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:2000639">
        <property type="term" value="P:negative regulation of SREBP signaling pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090089">
        <property type="term" value="P:regulation of dipeptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009302">
        <property type="term" value="P:sno(s)RNA transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030435">
        <property type="term" value="P:sporulation resulting in formation of a cellular spore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120174">
        <property type="term" value="P:stress-induced homeostatically regulated protein degradation pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031509">
        <property type="term" value="P:subtelomeric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000722">
        <property type="term" value="P:telomere maintenance via recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071596">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process via the N-end rule pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd23790">
        <property type="entry name" value="UBCc_UBE2A_2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.110.10:FF:000007">
        <property type="entry name" value="Ubiquitin-conjugating enzyme E2 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.110.10">
        <property type="entry name" value="Ubiquitin Conjugating Enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050113">
        <property type="entry name" value="Ub_conjugating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000608">
        <property type="entry name" value="UBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023313">
        <property type="entry name" value="UBQ-conjugating_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016135">
        <property type="entry name" value="UBQ-conjugating_enzyme/RWD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24067">
        <property type="entry name" value="UBIQUITIN-CONJUGATING ENZYME E2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00179">
        <property type="entry name" value="UQ_con"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00212">
        <property type="entry name" value="UBCc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54495">
        <property type="entry name" value="UBC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00183">
        <property type="entry name" value="UBC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50127">
        <property type="entry name" value="UBC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="6 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-0539">Nucleus</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0749">Sporulation</keyword>
    <keyword evidence="13" id="KW-0804">Transcription</keyword>
    <keyword evidence="12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 19" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="UBC core" evidence="20">
        <location>
            <begin position="4"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="18">
        <location>
            <position position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00039884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00042179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU362109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS50127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="EHS63125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="166" mass="18308" checksum="008D431C9EE4CB55" modified="2012-04-18" version="1">MSTASRRRLIRDFKRLSSDPPGGISGAPCPDNIMIWNAVIFGPADTPFEDGTFRLILTFDESYPNKPPTVKFLSKMFHPNVYANGELCLDILQNRWSPTYDVAAILTSVQSLLHDPNPNSPANAEAANLYRDNMKDYIKKVRATVEASWIDEGTIPGTANASSTTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>H6QP56</accession>
    <name>H6QP56_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_20769</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="12" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EHS63187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890742.1">
        <property type="nucleotide sequence ID" value="XM_003890693.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QP56"/>
    <dbReference type="STRING" id="418459.H6QP56"/>
    <dbReference type="GeneID" id="13542587"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20769"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_405501_0_0_1"/>
    <dbReference type="InParanoid" id="H6QP56"/>
    <dbReference type="OrthoDB" id="8922241at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.60:FF:000303">
        <property type="entry name" value="Zinc finger protein 41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47660">
        <property type="entry name" value="TRANSCRIPTION FACTOR WITH C2H2 AND ZN(2)-CYS(6) DNA BINDING DOMAIN (EUROFUNG)-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="6" id="KW-0804">Transcription</keyword>
    <keyword evidence="5" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="179"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="10">
        <location>
            <begin position="207"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="45"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="234"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="341"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="520"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="141"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="240"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="528"/>
            <end position="538"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="548"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EHS63187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="675" mass="73278" checksum="00158AC77F6E2B7D" modified="2012-04-18" version="1">MTPSSDGHQFIHRQSLSSQDGLYSNNNNNQELNTVQVTTSTTNISTPISLAPYPTGGISSDFNPTRPPLDFGQSHTNHNHGLDNTSNNIINHPTRVAGQPDDPHLSHSPMKKLSAQKTGNHHHKRTYSHPAGSDKKKLTGSRANQSTAAQSTPEISKNNGQQASPAANPPLKKNGKPLAKCPFCGMTFKKLEHCQRHERTHTLARPYSCTVCMKTFARQDTLNRHLRLHSRSDSKMAINKSAPAQPSQTPASPSKQPPRNLLSSPIRSNFDMLSVSGVPQPSFASPPHIHRRIARERGLSLSALEQLSLPSHLARDPSMINNGPRQSISLPLQRHHEQFAHPHHNPAYPFQYNQAPPPSSQLSLEISSQPSESSASYVYGEPIEARRGSWCPGGQNWNELTLLPPEDPGPPPPSTRGYRHQPQHLSLGVANWSKNLTNTFGLVEAGIGPIPQSAPATCTNWGTPLIGSGAPENTNPTAGSLVGPRWETFSQYESQPQIHPSWSGLGRLDEEEQIKMEPEPSINSAGTSQLLLSSPSQPGSFDQPGPFDQSNPIENNCPTGSTSTEISHEPQSESNPSVHWSFPVGGNLSDQYLGQLAHPTSFTSSSDPVDCFGNVWQSAPKNLGGVGSFHPGYWNPTACADNQDGHPTPIGDSGVLNPNGLPTKNPWQEIVEFDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPN1</accession>
    <name>H6QPN1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Golgi to ER traffic-protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EHS64133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890542.1">
        <property type="nucleotide sequence ID" value="XM_003890493.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPN1"/>
    <dbReference type="STRING" id="418459.H6QPN1"/>
    <dbReference type="GeneID" id="13542982"/>
    <dbReference type="KEGG" id="pgr:PGTG_20830"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20830"/>
    <dbReference type="HOGENOM" id="CLU_284428_0_0_1"/>
    <dbReference type="InParanoid" id="H6QPN1"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053094">
        <property type="entry name" value="Zinc_metalloprotease_ZmpB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193:SF2">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="142"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="428"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="559"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="185"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="568"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="582"/>
            <end position="630"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1092" mass="119705" checksum="4129E90F99089AD2" modified="2012-04-18" version="1">MVSTNTADGFTVPSPRRSARLATVEPLSPQKKKPMSPTEKRAHDLRNTPREDMELDVDVADKENTAAVGTNNSAAREMDWDGPTQNLSGQVGLTNFLGVGTQEACELEFGDISPSDLSAEHLGILAQMVHVNNQIHISLSAGSSLQSSTPLQPGSPPKTPTSPKQPKSPKKSKGKQKETSPAPSVENSIGFSPLSESHTDQLPVTSTPAAHTGEVMGFDFSSLGQSKKALPSDLEALVNKLVPMGTSTKVTGSINKEAAPVESNSARHPPAPVTQKKQVSLFMPNFLSNQVRHNLPSKTQTLAQLDREMKTMQLPDEAAMALMNVKELQKMKESLKKVADAQLLAFKEMKSASGMVAFPGVLDGLMKNARYITRFMNHLEDKVLEASRDGRQEIEVIEISDGEGEEVSIMPRRRSVAELKDFMRPGLVRLPSHLNPPTKRSASSSSLSQNMNKRAKSQDILLASDSSSTGNSYLADLVPQFEPGAQKTVTQLEMERRELAAIQEQEIIQESNASTPPPYNNSAKGFADPLAAAKAAFHRERRRAAAEAALAADLAEAATIPPPPVPASTLTTNSATVPASGPAQSVAPIPASSQSAPPAAASASQSTDAPRGPAQSTAPTPAASQSAPGQTTTAQEPAPSQPDNSQKTAPEAGTKKGKGKGKGKEAVPEQPPKKLRKDMTAEEKKRTTRERRRQSIWDMSKVMASADQYQRMIDNSKCTRWDFGPELLLKVRQMIEWSKNNTYRESFYTRKDLFQLESSTVQVLGVLSGRQYKSCLHKAVRLFQVDAEDPFWKPAVVNFKLLKEGFESKEDDKAHAWSVIGSMMCRMDDSHEFTPDGPTSKLISYGQSRLATLAADSYNQLHKFTVMGEPQAETGNKEDDMLASMSNAGKYIIGKIRCLKAGAAQTTGSNSRAGNGFLVIQRRIWETLLCILMMQQSRFLEECATLKAGHIWEDEVAKLTEDMVANDRGRSIFDKGDITTELTDKQRKDWGKLRLGAAYNQKDVWSLVMLAHARHEYLYTDGRYALRAPECTPWYTTDSFIRFVLRSANVTQSDPFKMDWDIAPRTFNKWVTDENIARFALHDILRECNLPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQ52</accession>
    <name>H6QQ52_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_21004</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="7" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EHS64653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890359.1">
        <property type="nucleotide sequence ID" value="XM_003890310.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQ52"/>
    <dbReference type="STRING" id="418459.H6QQ52"/>
    <dbReference type="MEROPS" id="S10.A47"/>
    <dbReference type="GeneID" id="13542782"/>
    <dbReference type="KEGG" id="pgr:PGTG_21004"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21004"/>
    <dbReference type="HOGENOM" id="CLU_1441714_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQ52"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="5" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EHS64653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="188" mass="20504" checksum="2E0FA791D6CB0155" modified="2012-04-18" version="1">MANAATLAVVKTALNHKNGCVVQLKNCANNGSNDVCQDADNFCAQYVSAPLLGNRSTYYYRSDVKRAIGAETQFSACQDNIGDDFWTTADAVRPTISSLGRLLDSGLRIVIWAGDKDFICNSLGVYRSLLATKWKYSKQFAKAPWKNLTVHGKVGGVYKTTGDFVSEPQYFHSNPSDGSQESKCTALP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQ86</accession>
    <name>H6QQ86_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21059</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EHS64801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890322.1">
        <property type="nucleotide sequence ID" value="XM_003890273.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQ86"/>
    <dbReference type="STRING" id="418459.H6QQ86"/>
    <dbReference type="GeneID" id="13542107"/>
    <dbReference type="KEGG" id="pgr:PGTG_21059"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21059"/>
    <dbReference type="HOGENOM" id="CLU_2360752_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQ86"/>
    <dbReference type="OrthoDB" id="168171at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324:SF2">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="105" mass="11946" checksum="30C544032A9F0C81" modified="2012-04-18" version="1">MNPLPNTLTPPITLDEPARRIGWNRDGVAGGPSSIQLLLLWITAGANYTQWFATPFHRQDRENACTEIQQFMRTQGNIHRDPRGTRSYSTGNRYSLIIIKVSQSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQD6</accession>
    <name>H6QQD6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21096</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EHS62548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890261.1">
        <property type="nucleotide sequence ID" value="XM_003890212.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQD6"/>
    <dbReference type="GeneID" id="13541195"/>
    <dbReference type="KEGG" id="pgr:PGTG_21096"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21096"/>
    <dbReference type="HOGENOM" id="CLU_2224492_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQD6"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="88"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="106" mass="11249" checksum="22ACCB1B0FD8A785" modified="2012-04-18" version="1">MHAPEAALTVRDGACGTGCRCQGKPQDGGVHRRGCRVGIHLCERGRRWDLSRLQTTMAGRGILPAIKPSTLDGQRDSSSAIDLSTSDGQKDSSPSHRDHTVSAAGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQR4</accession>
    <name>H6QQR4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HECT-type E3 ubiquitin transferase</fullName>
            <ecNumber evidence="2">2.3.2.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_21133</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.</text>
            <dbReference type="EC" id="2.3.2.26"/>
        </reaction>
    </comment>
    <dbReference type="EC" id="2.3.2.26" evidence="2"/>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EHS62812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890131.1">
        <property type="nucleotide sequence ID" value="XM_003890082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQR4"/>
    <dbReference type="FunCoup" id="H6QQR4">
        <property type="interactions" value="59"/>
    </dbReference>
    <dbReference type="STRING" id="418459.H6QQR4"/>
    <dbReference type="GeneID" id="13541465"/>
    <dbReference type="KEGG" id="pgr:PGTG_21133"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21133"/>
    <dbReference type="eggNOG" id="KOG0941">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_306771_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQR4"/>
    <dbReference type="OrthoDB" id="8068875at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000209">
        <property type="term" value="P:protein polyubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00078">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.2160.10:FF:000004">
        <property type="entry name" value="probable E3 ubiquitin-protein ligase HERC4 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2160.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2410.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1750.10">
        <property type="entry name" value="Hect, E3 ligase catalytic domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044611">
        <property type="entry name" value="E3A/B/C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000569">
        <property type="entry name" value="HECT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035983">
        <property type="entry name" value="Hect_E3_ubiquitin_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700:SF9">
        <property type="entry name" value="HECT-TYPE E3 UBIQUITIN TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45700">
        <property type="entry name" value="UBIQUITIN-PROTEIN LIGASE E3C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00632">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00119">
        <property type="entry name" value="HECTc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56204">
        <property type="entry name" value="Hect, E3 ligase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50237">
        <property type="entry name" value="HECT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 5" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="HECT" evidence="7">
        <location>
            <begin position="611"/>
            <end position="965"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="22"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="90"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="207"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="98"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="121"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="211"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="active site" description="Glycyl thioester intermediate" evidence="5">
        <location>
            <position position="933"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS62812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="965" mass="107951" checksum="B14EF9A555A7DB3F" modified="2012-04-18" version="1">MLDPLDPSCLTDHNHSELATGEIQQEAVQQPTTPTTPIRRSRTGNRRSIGLTLSPKSSVVPVDCNLPANPSHSKLSVSASSFKRRSLNLANLLRHPTSMRNQSSSTTGHRSPTNPEHYRQPFPPVTPIHPTPPPLPSRPTNLKSSSSARKSLPPNLQLRPGPPPESPVTCPCCSRSITLDHSRLSQPARCPTCAIIWDSPDSFLVSPKIDQSTRSDEDRLYTSQTSTASEHPASSILTITEEQVAQLHSLSLSHEQLESFERDPSAFLASVSPNSALDRLIKEFDRLALELLTKLFSSIANLSTAFLAKPTTDKQPKPRHQAHQAVQIELVNSFFQAICGRPTGRDILLSQLYGFLNRPGTQSVSILASLKSPNEPQQTVDDLIQWVWAIVLLQCPILSPSCKIAPQKRLVITARTLGLISSLPNRLHHRLVSFYSHPSCPTKLFAEKVELVNVFISDRIRHFLQLPDGFGAGLEPQSVHHLPKYMYDWSLLASSRMMALLCGSNLQSRKLPPTSFYNVSTDLIQPIDLVYDFECWESRKSSYTLCGYPCLLSMSSKISLLTYDGKRQMGLEARQAFIDSLLGRSLAPPVLPLSIRRSHLVEDSLRQIASSQSELKKLLKITFVDEEGVDGGGLKKEWFLLLIRQLVAPEYGMFLHDQDQHQIWFNPASQELEEFKLIGTVLGLAIYNRATLDFGLPLIGYRKLLGFSVNRLSDLATLKPEVAKSLRWLLEYDGDDFEEICSRNFVGDYDAYGTVVEVPLIPNGENIPVTKSNRAEFVKLYCDYILNKSIEKQFQAFSEGFNSIAAGNGLSLFQPEEIELLVIGSTYDSKLAIEDLKAITHYEGFQPTDLTIQYFWIVVENFGFEDQKKLLRFITGTDRIPATGISGLNLKITRSVRSSLDSNPTNNNRRRRHLSNQLRSMAFNERLPESHTCFNQLILSDFSSVQSLDQKLRLAINESQGFGLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQY7</accession>
    <name>H6QQY7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21274</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EHS62924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890084.1">
        <property type="nucleotide sequence ID" value="XM_003890035.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQY7"/>
    <dbReference type="GeneID" id="13540887"/>
    <dbReference type="KEGG" id="pgr:PGTG_21274"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21274"/>
    <dbReference type="HOGENOM" id="CLU_087680_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQY7"/>
    <dbReference type="OrthoDB" id="2497916at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS62924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="192" mass="22430" checksum="779CCB99450AF06B" modified="2012-04-18" version="1">MESRYKDRIRDLTKEAFLRHDIQLYTRGKYKPGTSDVSLLRVVMMWQDSLEKLPLEFRRRELPPNYDTDAQTKKELIGVVREIQRRVRLMIRERLLDGIVQSNGQVAEDGLVPSLYELCETIYRFLHPGEASMSKATVRKNITILWAGRIGHLRLQTVDHLIHPQLSKVSQWGLIDEKLKELRARGTDYTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQZ2</accession>
    <name>H6QQZ2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21339</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EHS62971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890054.1">
        <property type="nucleotide sequence ID" value="XM_003890005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQZ2"/>
    <dbReference type="STRING" id="418459.H6QQZ2"/>
    <dbReference type="GeneID" id="13540910"/>
    <dbReference type="KEGG" id="pgr:PGTG_21339"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21339"/>
    <dbReference type="HOGENOM" id="CLU_2122259_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQZ2"/>
    <dbReference type="OrthoDB" id="10279504at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="114" mass="12182" checksum="6A41C8C753B27D8A" modified="2012-04-18" version="1">MDTELAGIWSTLIVSTAKTLPAGRGPWFGLGAGLLKKGEFEIKIIKHPKPTKASSPSEANARPTEPLSPSFPRVFAGPLILTSAADGIHPGKYTHQDFTLLQTGYDRLTVQIVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRZ1</accession>
    <name>H6QRZ1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21559</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EHS63426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889711.1">
        <property type="nucleotide sequence ID" value="XM_003889662.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRZ1"/>
    <dbReference type="STRING" id="418459.H6QRZ1"/>
    <dbReference type="GeneID" id="13542816"/>
    <dbReference type="KEGG" id="pgr:PGTG_21559"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21559"/>
    <dbReference type="HOGENOM" id="CLU_1678788_0_0_1"/>
    <dbReference type="InParanoid" id="H6QRZ1"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034719">
        <property type="term" value="C:SMN-Sm protein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097526">
        <property type="term" value="C:spliceosomal tri-snRNP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="157" mass="17768" checksum="3A140379DE0FE86E" modified="2012-04-18" version="1">MSARTDVARRVLLCATTGLLNSSDKVCPKTRRTGLSDELVYAVSSENKKIVIIWKVINKDLDAFKAEVIRGIKSEEDKFLGFFVEHQENARNIFWNVVIPFGGAFAGNHKRRLDSVETFKDFLLVAEGTSETRKIVCRLVQKDPKDIAKVCFLNFTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>H6QS42</accession>
    <name>H6QS42_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21691</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EHS63515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889660.1">
        <property type="nucleotide sequence ID" value="XM_003889611.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QS42"/>
    <dbReference type="GeneID" id="13542870"/>
    <dbReference type="KEGG" id="pgr:PGTG_21691"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21691"/>
    <dbReference type="HOGENOM" id="CLU_009176_2_1_1"/>
    <dbReference type="InParanoid" id="H6QS42"/>
    <dbReference type="OrthoDB" id="5424058at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="299" mass="33577" checksum="73C3C94DE5924CA5" modified="2012-04-18" version="1">MTPKEFITGLLKKDHIELNYRRRTWGTTYGASSTIELVSEIAKIFHKKDAARHFWANFIQAEVKLVHYQDVVLLCRQEMESHSSGSFMSSRLVRRDFFGLAAKEARNHKLTTVEQPMLYNVIHGILSHLDKEEGNSVHVDDPHDDSTCLQENTLTHDTNNSRGHMGNTPTDIGETVVEELVAEAEPEVHLKSTSGSLEEVMSEYGGFSYSSKEDGPDGRQKKIKHIATVICSMLSFACNHRHNGLQLENSIRFYACGVSETVNEYLHYWGLTSHRKTAIHALQSLASEAADTIASSMAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSW2</accession>
    <name>H6QSW2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_21889</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178300">
        <property type="protein sequence ID" value="EHS63851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889412.1">
        <property type="nucleotide sequence ID" value="XM_003889363.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSW2"/>
    <dbReference type="GeneID" id="13540617"/>
    <dbReference type="KEGG" id="pgr:PGTG_21889"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21889"/>
    <dbReference type="eggNOG" id="KOG4585">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018552_2_0_1"/>
    <dbReference type="InParanoid" id="H6QSW2"/>
    <dbReference type="OrthoDB" id="2502344at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF85">
        <property type="entry name" value="GH03217P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="8">
        <location>
            <begin position="220"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS63851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="432" mass="49317" checksum="47A2640FD1628AEF" modified="2012-04-18" version="1">MHKYSERQQILRDLFMVLIFLHHEETDRLLDSSMNIPSVPSLGSIAAPENPGRALVLDMLFDDEAMVSDLIRLILSNRYLNNRLPGRTHNEYDLALLFDMREEDFKQAVRTTKTGFTWLLEQIYFNPIFYSNSPRPQLPVPHQLALTLERLGSNGNGASVGRFSRNLSVGRGTVIKASRRVIRAINDLSQRYLLWPDTDRRDEISKVMKAEGFEGCIGFVDGTTIPLYQRPSIDGEVFFDRKKRYSINCQVICDCDQFITAYMTGWPGSCGDSMVFKRMMVHKEPALFFDPGQYLIADSAYELGLHCIPAYKAPAAYIQENTEFNYCLARSRVRNEHTIGILKGRWASLQHLRLAIQKPSDMMEIIRWVNCCVTLHNMLAHLGDAWDNLERPIEDVPATGPRGSPEDTATEHRNIVQAHCLEKNYALGVLPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>H6QU61</accession>
    <name>H6QU61_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22355</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178330">
        <property type="protein sequence ID" value="EHS64475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888963.1">
        <property type="nucleotide sequence ID" value="XM_003888914.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QU61"/>
    <dbReference type="GeneID" id="13541117"/>
    <dbReference type="KEGG" id="pgr:PGTG_22355"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22355"/>
    <dbReference type="HOGENOM" id="CLU_011079_0_0_1"/>
    <dbReference type="InParanoid" id="H6QU61"/>
    <dbReference type="OrthoDB" id="2496257at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="542" mass="62990" checksum="F3963534A3C584E3" modified="2012-04-18" version="1">MGSERMESKKHIKSLKPKNDQEFILIKSQLQKLYHSLKGPTIDRVFRLSGLNPPIDFLLPKSNPKRRDPKSRKIFKKVFWFDRLSSGGRARTKDEKPSRSPTVPSTFMVGDGIEFKNLKSYSPIEVPHEDRSGAVKDNTFSESYPADLEFSGKAMRISQGEYPRTNVPMQVPRQDGSLMSGKEEGVSLENVKIQRTGTAEVGGDELSYINDKIINETVDIWNFLQGKLIAFSKAPSDPKKAKFQLAFLKSLFQLGDYIFRYGLLPPAFIESIEIFKPKTLSEMVKFHIDLLFLKHGPKFFVAKDSVVPQLEFLTNSLALKHFHRSIKALSAEDQKYPVYLALTTILQHMEECFPESESSPDFRLIAQGFRLSEFLEQADRLSLQLHEAPGIEHLNRNDNVLMFELTESFFACFQHPTLTVAQSRIQFQMVYYMLEFVDQYYKPMTAAILAQWGSFCRIQQKLKFMNLYLKFFRDRDQYPSSMYENLDMSFLEMLDTLEIWIKYRIKEIFNKKNWLEISGTVPKVKFNLWMSEIKKPKFPFFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>H6QV24</accession>
    <name>H6QV24_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22632</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178369">
        <property type="protein sequence ID" value="EHS62637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888642.1">
        <property type="nucleotide sequence ID" value="XM_003888593.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QV24"/>
    <dbReference type="GeneID" id="13542286"/>
    <dbReference type="KEGG" id="pgr:PGTG_22632"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22632"/>
    <dbReference type="HOGENOM" id="CLU_983981_0_0_1"/>
    <dbReference type="InParanoid" id="H6QV24"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052980">
        <property type="entry name" value="Crinkler_effector"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33129:SF1">
        <property type="entry name" value="ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33129">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS62637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="283" mass="31996" checksum="0949A0E387EBA64F" modified="2012-04-18" version="1">MLLGCKFFEANNITYNKLVVTGTPGIGKTVFSAYYAACAGKTVVWEPLILPGADPVTYLMTTSSGVELLDTKSSTLSTLSRNPTQYTLLMDWVFKWYGGVPRHVLELATTAFKEHHGDEDKALKALVQGMNQAIHKVTPVESLDNFTWHGHCPKLSMKWWSDLHRNFELTFKTFSKLLPMVAITVDWYLRCTLKACCKKAEDFKSVNLVKEIHKLARALQSHFLKLGVKPSKLTKKLTYAPMCSGSRAVQIWHQLICCVDQQMFFKLLFPTHIQSSMRASTKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>H6QV74</accession>
    <name>H6QV74_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_22663</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="6" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178378">
        <property type="protein sequence ID" value="EHS62735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888592.1">
        <property type="nucleotide sequence ID" value="XM_003888543.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QV74"/>
    <dbReference type="STRING" id="418459.H6QV74"/>
    <dbReference type="GeneID" id="13542344"/>
    <dbReference type="KEGG" id="pgr:PGTG_22663"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22663"/>
    <dbReference type="HOGENOM" id="CLU_723877_0_0_1"/>
    <dbReference type="InParanoid" id="H6QV74"/>
    <dbReference type="OrthoDB" id="2507294at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <keyword evidence="2" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="CCHC-type" evidence="4">
        <location>
            <begin position="349"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="98"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="18"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="116"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EHS62735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="382" mass="44157" checksum="C8483509DB03E77C" modified="2012-04-18" version="1">MENTGDNVHSTKNEHSRIQNGNRETTTLPDRDPSHNDVMLFGISHEIKQLAEAVRSKNESCVNKSSVEQMIRDLSEEHRRDFSSLSTKLDQLLSQFGDDGAEAAQPGSNFRRRNDQYSIQPTSSNNFRNPQLTNVQSVGISEMQKQLWAAVNKLDWPKFSGQGEYDHTEFIDWIDDCRDETSIPDEYIQVKLISILTGVASQWYKTMRKDHKDEKWDFWREEIRKQYGTSNWMRKKQESFEKDRFIPGETPVSSWVTRQYRRLRAFEPQLSQQSINFRLLGLMDREVEYATKNAMQSTTADMSTLINVLEDIVDKTRLGRKRFPPKSIEKTPSKVIEGREKAPVTSDIKCYSCQKTGHTSCNCPSKVNNVGEPAKNFRARAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>H6QVJ2</accession>
    <name>H6QVJ2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22782</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178451">
        <property type="protein sequence ID" value="EHS63268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888472.1">
        <property type="nucleotide sequence ID" value="XM_003888423.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QVJ2"/>
    <dbReference type="GeneID" id="13541004"/>
    <dbReference type="KEGG" id="pgr:PGTG_22782"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22782"/>
    <dbReference type="HOGENOM" id="CLU_3078697_0_0_1"/>
    <dbReference type="InParanoid" id="H6QVJ2"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="8"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="52" mass="5452" checksum="A9F6FEE333C73D24" modified="2012-04-18" version="1">MRGLSIEQSQQAQGTSGSQRPPDTQQQNAPTPPPNAQPSSAANKDNPSPPQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>E3JR32</accession>
    <name>E3JR32_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Urease accessory protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_00386</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MYG1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307436.1">
        <property type="nucleotide sequence ID" value="XM_003307388.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JR32"/>
    <dbReference type="FunCoup" id="E3JR32">
        <property type="interactions" value="1047"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JR32"/>
    <dbReference type="GeneID" id="10528141"/>
    <dbReference type="KEGG" id="pgr:PGTG_00386"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00386"/>
    <dbReference type="eggNOG" id="KOG2948">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051576_0_0_1"/>
    <dbReference type="InParanoid" id="E3JR32"/>
    <dbReference type="OMA" id="FHCDEVV"/>
    <dbReference type="OrthoDB" id="10265310at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003226">
        <property type="entry name" value="MYG1_exonuclease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11215">
        <property type="entry name" value="METAL DEPENDENT HYDROLASE - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11215:SF1">
        <property type="entry name" value="MYG1 EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03690">
        <property type="entry name" value="MYG1_exonuc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010105"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP74430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="354" mass="39455" checksum="80C8E03A4F034BD6" modified="2011-01-11" version="1">MASENVSSGEVKKPRLESPLIGTHSGTFHADEALAVSLLRSLEKFKSSRLVRTRDPAVLETCDIVVDVGGQYDPARHRYDHHQREFSETYSGDHRTKLSSAGLIYKHFGSEIVASHLGLPTDNPTVPILVAKLYDGFIEAIDAIDNGIERYETVDQDGKPTNLPVKLRYQSHTDLSSRISHLNPAWNEPVNNTILDSQFEKASSLAGSEFFSRLDYFSKAWLPGRELVVKALENRFTNQAQDKFGRVLIFDSFCPWKDHLHTLEKTTLTESSERPLYVLYADESQNWRIQAIPVTPGGFESRKALPEAWRGVRDAALSELIGIPGSIFVHASGFIGGNQTFEGALKMAQKALEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRE7</accession>
    <name>E3JRE7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF4219 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_00698</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307748.1">
        <property type="nucleotide sequence ID" value="XM_003307700.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JRE7"/>
    <dbReference type="GeneID" id="10546593"/>
    <dbReference type="KEGG" id="pgr:PGTG_00698"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00698"/>
    <dbReference type="HOGENOM" id="CLU_063284_1_0_1"/>
    <dbReference type="InParanoid" id="E3JRE7"/>
    <dbReference type="OMA" id="YKANHAE"/>
    <dbReference type="OrthoDB" id="2504515at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025314">
        <property type="entry name" value="DUF4219"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13961">
        <property type="entry name" value="DUF4219"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4219" evidence="2">
        <location>
            <begin position="18"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="196"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="229"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="260"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13961"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP74742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="273" mass="30874" checksum="559EFAC82F967079" modified="2011-01-11" version="1">MAGLAISLKDELSMLPVLTGEANYPMWTKRMRTFLKNKELWAVVNRDPGAAPTARILKQLSDAGHVITMKIGDRVYNGLVTEVNEDNGYLLWKKITQTYSRYTTHRYTKCMTTWHAIRYEGRTLEFIKNIEDALDAFAAIAHPIPSKDICSIIVSKISITRRALTDSFIFNPELMENHEMLIDRLRDIAEEEDDNIKRKGGLEKTAVALANNTRTRPPPRCRNGQHNPEATHPEERCWSAHPETRIVKNPRPKPSQHHTSAAQPSASPANAPG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRH9</accession>
    <name>E3JRH9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">RanBD1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_00485</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
            <location evidence="1">Nuclear pore complex</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307535.1">
        <property type="nucleotide sequence ID" value="XM_003307487.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JRH9"/>
    <dbReference type="FunCoup" id="E3JRH9">
        <property type="interactions" value="111"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JRH9"/>
    <dbReference type="GeneID" id="10528093"/>
    <dbReference type="KEGG" id="pgr:PGTG_00485"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00485"/>
    <dbReference type="eggNOG" id="KOG0864">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_414507_0_0_1"/>
    <dbReference type="InParanoid" id="E3JRH9"/>
    <dbReference type="OMA" id="SDGMGHI"/>
    <dbReference type="OrthoDB" id="185618at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051028">
        <property type="term" value="P:mRNA transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053074">
        <property type="entry name" value="NPC_Nucleoporin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015007">
        <property type="entry name" value="NUP2/50/61"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000156">
        <property type="entry name" value="Ran_bind_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38697">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN SIMILAR TO S. CEREVISIAE NUP2 (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38697:SF1">
        <property type="entry name" value="NUCLEAR PORE COMPLEX PROTEIN SIMILAR TO S. CEREVISIAE NUP2 (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08911">
        <property type="entry name" value="NUP50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00638">
        <property type="entry name" value="Ran_BP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00160">
        <property type="entry name" value="RanBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50196">
        <property type="entry name" value="RANBD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0509">mRNA transport</keyword>
    <keyword evidence="6" id="KW-0906">Nuclear pore complex</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0811">Translocation</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RanBD1" evidence="9">
        <location>
            <begin position="530"/>
            <end position="657"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="204"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="439"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="23"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="112"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="231"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="335"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="393"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="461"/>
            <end position="482"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="483"/>
            <end position="501"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP74529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="658" mass="68925" checksum="C9F884E9853A1D40" modified="2011-01-11" version="1">MVKRQAEKQLTDRNCDDVEPDDTPSTGVLKTASLQAISKRQIRALPKKTGASVSKPASQFGAVPPFPANGKPASTLFGQAPQSFNPTPSFNLFGGSSSFGTTKPTAFGTAPSAMSNSPNSTDPSNKQELITYYSSLRGLNLSVTKAFEELIKKDAFMDLGSAFDHVKQKYNEHRREIEAQLERSQPSQTDKAITSVTAEAPVKSPFNFASNEAPKVSSTGPTLSAGGEARTTPSLFSFSPSNAQKSSEGSSSVENPTVSAPEVHPSSDEADNLSLPSANGSAAPGAKAGSRPGAFTMAAPMRPSPLRYESQTSPADSPAPESAEEPAKSAFSLAPITQASKPESSLSSTANTADQQPNPKEANTDDSSTVPLPLSESKSKTTSLFGVTGPSAAGTSAPSPFFTSKPLSYTSLSAQNSTAKPSSIGFSFGIGAAGNGSAFGGFGSPKSAPGQATPPKNAFNPVGFSFGSSPPTSSVFSTPKPSENTSTEPGSTNIISTSTGNEKPDAGSENLAGEEGSDDLGTSSTEAKAVDSKKGEEDEETLFETTGRVYALLDKKQEDGKVQKGWVGWAICNVRLNRHKETQRCRILARSQVNQGILINFFVRPDLKPEDRGNSLEVLGFNPEGSHLQAYRVRPASDQVVKDFHKAITEVVESLAKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3JSL9</accession>
    <name>E3JSL9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01637</name>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75044.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319463.2">
        <property type="nucleotide sequence ID" value="XM_003319415.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JSL9"/>
    <dbReference type="GeneID" id="10547406"/>
    <dbReference type="KEGG" id="pgr:PGTG_01637"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01637"/>
    <dbReference type="HOGENOM" id="CLU_045924_0_0_1"/>
    <dbReference type="InParanoid" id="E3JSL9"/>
    <dbReference type="OrthoDB" id="10499879at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="151"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="202"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="206"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="223"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="314"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75044.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="325" mass="34921" checksum="033EEDAA13D3064F" modified="2012-04-18" version="2">MAPKSTPTTENIRHVEAASDDSVAASLPPFEVPFLAMTSHEFKRAVIAALGASRTDFPLSDVLTAADEARKLQWRGNISWPGGPQTDNFNVTLSYKAFGEHVAQAVLPKRCRLQLVMENPLRRIADPYALASEVYVPFRPNGHDLPPPNHCFVLQPPAPFTEGGIEPPTSTRDGTPPTAGGLDAQRSAESSPCCLLRGYSSSATGDEVHEPEFARASTHESSRQQSAHPNLGRSSESPPPLADDNAAKSPPPLSSSPGLIMLDGPPSSWVSNTNIRPRLRRRVSSSPDVEVVKSSKRTKTITPNENGIPPEVGTSESSVLRNSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3JSP9</accession>
    <name>E3JSP9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_01667</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319493.1">
        <property type="nucleotide sequence ID" value="XM_003319445.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JSP9"/>
    <dbReference type="GeneID" id="10547436"/>
    <dbReference type="KEGG" id="pgr:PGTG_01667"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01667"/>
    <dbReference type="HOGENOM" id="CLU_604297_0_0_1"/>
    <dbReference type="InParanoid" id="E3JSP9"/>
    <dbReference type="OrthoDB" id="2496674at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP75074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="453" mass="53335" checksum="8FB03B8CA8B92994" modified="2011-01-11" version="1">MKQTLLNKDIVDKINVDEVLSLEDTFYIPFEEELQKSNGKSRYEWMTKKGKLVEGMKAQDFQEACRIFGEERAAKIAAADVNLRIANYMGPLYVALSDDKLQLILSSSFHNGAFEVLEYYWENHPKEFQKVESSIAETFERFEGARRVITARKLLQKHVLADNWLEFREDALENRKLSEYQLRGLESAWRISINPAEIKNIRDPFGDQNVKIEVLIGRKEYMRWKALEKYLIGRRDLVEWWVNEPFSLENESLKFGLDVTTILQVGDALQFGHNGWSHLRKSNDRIDSEYANLLNIVLNEKGMLPWPESAERIWLARLPEDLYRQRFQEISRYIWTSEEPKKGSVKVESFPKSKNVLLEHLNHKLVNSQDSHLWFELLRSLCNDHEHIDLEGFLRKMKVWKAPYYKFHSQIHLTDDEKSALVAWLTKIPRYNPASKSNVRSLARIKHIFPSIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUQ0</accession>
    <name>E3JUQ0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_01106</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75775.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320194.2">
        <property type="nucleotide sequence ID" value="XM_003320146.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JUQ0"/>
    <dbReference type="GeneID" id="10543767"/>
    <dbReference type="KEGG" id="pgr:PGTG_01106"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01106"/>
    <dbReference type="HOGENOM" id="CLU_043575_0_0_1"/>
    <dbReference type="InParanoid" id="E3JUQ0"/>
    <dbReference type="OrthoDB" id="2500587at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP75775.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="490" mass="56792" checksum="C9672D2DD4FF7D8B" modified="2012-04-18" version="2">MNKQTNRGAQVDNVAHTLYIHKPVSTKHKSPNKQRPHPKMMNPIEIEEQSFSEDFDQYSNSSIESIADNPSEVHRYRAQGDRVLSKFRGIIDKYDYHIGPDHPPIQTSPRKSKITDQEIEKKKELLERLDEVLLPELSLEIQGFSDLFEPSELNKAPYRQLKTIRRRQHELEKTIDRVLKAKREICRDACTTSEPNDPDLEEIKSYRLYILAYGFQVYLIPMICELFIKSYELIEEMAFSNDPPDHIMDVSTARGLMLDCTAEATTEIAAIRRFIQGSDMDNIQSRWSGLVPAIEETHKAYEDLIKKVQLHPVLCRSYTPVDPSIKVAKAFLPIMKLSRLFFKKLASDPKFSNNDKRLNKFSHLPSSQLSALGELPDVLDKVLAESHEHLENSSITEEGMTSWELSLLVDELQVGFRQSMIVVDYFISQIPDSDREGFPKQTRYREWFELWNDTFSSSVSKLMQVVDPYLYEDCQCPNCLNPRPRPHQPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3JV73</accession>
    <name>E3JV73_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">GPN-loop GTPase 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_01279</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 8">Small GTPase required for proper nuclear import of RNA polymerase II and III (RNAPII and RNAPIII). May act at an RNAP assembly step prior to nuclear import.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterodimers with GPN1 or GPN2. Binds to RNA polymerase II (RNAPII).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the GPN-loop GTPase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75948.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320367.2">
        <property type="nucleotide sequence ID" value="XM_003320319.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JV73"/>
    <dbReference type="FunCoup" id="E3JV73">
        <property type="interactions" value="704"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JV73"/>
    <dbReference type="GeneID" id="10533026"/>
    <dbReference type="KEGG" id="pgr:PGTG_01279"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01279"/>
    <dbReference type="eggNOG" id="KOG1534">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_037460_0_0_1"/>
    <dbReference type="InParanoid" id="E3JV73"/>
    <dbReference type="OrthoDB" id="5839at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007064">
        <property type="term" value="P:mitotic sister chromatid cohesion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006606">
        <property type="term" value="P:protein import into nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd17872">
        <property type="entry name" value="GPN3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000552">
        <property type="entry name" value="GPN-loop GTPase 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004130">
        <property type="entry name" value="Gpn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030228">
        <property type="entry name" value="Gpn3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231:SF7">
        <property type="entry name" value="GPN-LOOP GTPASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21231">
        <property type="entry name" value="XPA-BINDING PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03029">
        <property type="entry name" value="ATP_bind_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="264"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="264"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00054449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP75948.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="283" mass="31848" checksum="921A4F41C0B22284" modified="2012-04-18" version="2">MRYAVLVCGPAGSGKSTFSSTLITHAQTIGRPIHLFNLDPAAEEFEYEPSIDIRDLISLEDVMEELELGPNGGLVYCFEYLLKNLDWLQENLNSYDDDFLIIDCPGQIELYTHFNIMQKIVQVLTMEFDFRLCATYLLESNFIADRPKFFSGVLSATSAMINLEIPHINVLSKMDLFKTGRTAAGTIAQIGPKELERYLDPDPDLLLGEVNEKTNPKFHSLNQAISHLIQDFNMVSFLPLDITDDESIGSILSHIDHAIQYGEHEEPKEPHDMDGGDFNAADE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWK9</accession>
    <name>E3JWK9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02875</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP76434.2"/>
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    </dbReference>
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    <dbReference type="HOGENOM" id="CLU_136935_0_0_1"/>
    <dbReference type="InParanoid" id="E3JWK9"/>
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    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76434.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="129" mass="14993" checksum="D8A56A75233ACB42" modified="2012-04-18" version="2">MEETLRNEALQAIDFLERHKRANTDKLYREALTQEPKKDRYVDTNSKVKSLKEMVARDLGFQGTVKHPRVWHLQTTDVGAPMKNHGTPPAPRRDTQVQVQLPRANHSAMLAFILLSYALLFFLLFSDWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>E3JX03</accession>
    <name>E3JX03_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">HTH APSES-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_02039</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76578.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320997.2">
        <property type="nucleotide sequence ID" value="XM_003320949.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JX03"/>
    <dbReference type="FunCoup" id="E3JX03">
        <property type="interactions" value="136"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JX03"/>
    <dbReference type="GeneID" id="10528435"/>
    <dbReference type="KEGG" id="pgr:PGTG_02039"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02039"/>
    <dbReference type="eggNOG" id="ENOG502QPWC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009666_1_1_1"/>
    <dbReference type="InParanoid" id="E3JX03"/>
    <dbReference type="OrthoDB" id="6718656at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030907">
        <property type="term" value="C:MBF transcription complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033309">
        <property type="term" value="C:SBF transcription complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001228">
        <property type="term" value="F:DNA-binding transcription activator activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045944">
        <property type="term" value="P:positive regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.260.10:FF:000001">
        <property type="entry name" value="APSES transcription factor (MbpA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.20:FF:000347">
        <property type="entry name" value="Transcription factor, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.20">
        <property type="entry name" value="Ankyrin repeat-containing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.260.10">
        <property type="entry name" value="Transcription regulator HTH, APSES-type DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin_rpt"/>
    </dbReference>
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        <property type="entry name" value="Ankyrin_rpt-contain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036887">
        <property type="entry name" value="HTH_APSES_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018004">
        <property type="entry name" value="KilA/APSES_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051642">
        <property type="entry name" value="SWI6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003163">
        <property type="entry name" value="Tscrpt_reg_HTH_APSES-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828">
        <property type="entry name" value="ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43828:SF3">
        <property type="entry name" value="CHROMO DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00023">
        <property type="entry name" value="Ank"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04383">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00248">
        <property type="entry name" value="ANK"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01252">
        <property type="entry name" value="KilA-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48403">
        <property type="entry name" value="Ankyrin repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54616">
        <property type="entry name" value="DNA-binding domain of Mlu1-box binding protein MBP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50297">
        <property type="entry name" value="ANK_REP_REGION"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50088">
        <property type="entry name" value="ANK_REPEAT"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51299">
        <property type="entry name" value="HTH_APSES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0040">ANK repeat</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="HTH APSES-type" evidence="6">
        <location>
            <begin position="43"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="344"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="repeat" description="ANK" evidence="3">
        <location>
            <begin position="465"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="141"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="725"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="553"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="8"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="146"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="208"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="241"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="725"/>
            <end position="755"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="758"/>
            <end position="767"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP76578.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="833" mass="92076" checksum="742AB7059E63444B" modified="2012-04-18" version="2">MAAHKTTNDIPVSSSHHINPESGTGTSSTQAFPIPNIKNNPHVYMAVYSSVPVYEMMVRGIGVMRRRSDSYMNATQILKVAGLDKSKRTRILEREIIQGEHEKIQGGYGRYQGTWVPFTRAQELATQLNVAQLLAPLFDYRPEPNSEVNIRSTNTKPSSSASRANSHKTTLARQTSRQSLNEKRERSGDTTPLPHDPPEAGPSKRSRLNTPSRQSNGSANTPSSLIDHSHSAMDPDFIIPHSQSQPTAASQCTTSTFAPIHGATVEYPAGPSHLRKSNSSSRSHLEVALKAERNIHTLMALFSNPPDGDELESETHHENPNSVAEVNEVLEDPELEIDTPIDEHCHTALHWASSLARLGLVRAFLRSGADVNRGNDVGETPLMRSTLVTNNFERESFNQLLELLHPSLWTLDNQDRTVLHHICLTASIKGRGESSRYYLECICEWIVNKHGAQFDSQLFDAVDLNGDTALNIAARVGNKHLVRMLLDVGADMTIGNNLGLKPIDFGVGAGETSASYTDDMISAPLRRNPTASAPARSSRDIITSITSSVNSLSEDFENEIRSKTDRLESVRAQLMVATRQLTTQRRQLESLKHDLDERALLELRLKKLRMAIAEEDGFDWTGRSDLDGRPAQAGKLFEQNGIASTLAGLSASQIQLELEPDPFIPPENNQDSLVYLRRLEKWYVRVLSLLRERIGRMKGSNLEQEAKYLKVIGSFIGNTCTNDLSSSGSSMTGRPANQTTSTTQEVPSRATQNVNPADIHDLESMDGHRRKVSTTDAVNKSHEFGRTRSELLKASMIDNKLLKQLMAAIESDGPELDLNRVAGFMQRVQSGSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3JX48</accession>
    <name>E3JX48_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">NuA3 HAT complex component nto1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02084</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76623.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321042.2">
        <property type="nucleotide sequence ID" value="XM_003320994.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JX48"/>
    <dbReference type="GeneID" id="10528560"/>
    <dbReference type="KEGG" id="pgr:PGTG_02084"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02084"/>
    <dbReference type="HOGENOM" id="CLU_370507_0_0_1"/>
    <dbReference type="InParanoid" id="E3JX48"/>
    <dbReference type="OrthoDB" id="2507607at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="65"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="318"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="531"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="189"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="324"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="343"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="356"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="381"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="533"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76623.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="751" mass="83445" checksum="0D306FD56F4F712B" modified="2012-04-18" version="2">MATTGLYTLNDPVQQDHPNYGPPLDQANPITPSVPIHQLQDHQSSYDPYRDPTASLATHYIECSRDSSGSNTNNGQDSHLASNDSSPFGSNGNQLTPNMDANTNFQAIMTAIDDPSTQPNQPLASIEPDPSISNAKPANHANSHDPALDEDAPHEEDDDEGSDNDSSFINPPVLTGDTYQDGSIDPNHPLTTSNLHSWSHPQTSHHQEIEQKPEIPSSSEPLSTRKRKRTSDQNGSVKTPARRESGRPKQTPRVKSAEQVAREALIEQQNPHASKNELRSLKLRALHAIRIAERELNMPEAEKQRRLRLREYVRHKRAEERSLKGSGKTGTTGAEAELKPRSAKSGSRSSSQSAKIKQEVEPSDHQEEPQATNSHLPMAVSYPNQSQQLPSPSAPSPMNDLPDNQLAPNGHTSNSKRKAAPKKSEKPPKGKSAPRAKRSVASLEAEIKRRNPNASPTQLKSLKLRALHADRIAEREQNMSEKDKIRRQKLREYMRRRREEERVEKGQAPTELSSYIEAKVASDSTLRYRRSLDSSTSMNEESQVNPDDSSMSGLTAAPLVPEGMNADDRLAAFSMMELTNNYQHQFAQPHHVPVPSPNLHLHPLFQMPMGQPSIEVPSPYDSIHTGYPAMTEQWAHVPQPDPSSLPADEMAEKALHAALEEAIYMKPHDHNQIPLGEQQQQLHHHHPHYSPTEQSIEADRLARLEQERIQLAEDEILGIERARELAMIAAAAAEVEHAQQISGSTSSHRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3JY50</accession>
    <name>E3JY50_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_02436</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76975.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321394.2">
        <property type="nucleotide sequence ID" value="XM_003321346.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JY50"/>
    <dbReference type="GeneID" id="10529242"/>
    <dbReference type="KEGG" id="pgr:PGTG_02436"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02436"/>
    <dbReference type="HOGENOM" id="CLU_126700_0_0_1"/>
    <dbReference type="InParanoid" id="E3JY50"/>
    <dbReference type="OrthoDB" id="10289520at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP76975.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="151" mass="17115" checksum="02F570E5FEEB32A5" modified="2012-04-18" version="2">MRVYEPSLNCEFYRDRVRSALELRASLSVGPPRSKLLPTSHQFGTRLLNYSDYRLSSCYSDYPSPPLSIDSRLSPRRVSGYLQLPVVLRCFSLIDPGFASIVFCIHDLVVLNKGCKTPWTVRKRKAAILNMFNPLISWHSIGSETSTDLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3JYG7</accession>
    <name>E3JYG7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_03048</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the short-chain dehydrogenases/reductases (SDR) family.</text>
    </comment>
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        <property type="protein sequence ID" value="EFP77092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003321463.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JYG7"/>
    <dbReference type="STRING" id="418459.E3JYG7"/>
    <dbReference type="GeneID" id="10529440"/>
    <dbReference type="KEGG" id="pgr:PGTG_03048"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03048"/>
    <dbReference type="eggNOG" id="KOG0725">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010194_1_0_1"/>
    <dbReference type="InParanoid" id="E3JYG7"/>
    <dbReference type="OMA" id="SIAQTCA"/>
    <dbReference type="OrthoDB" id="4131217at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05233">
        <property type="entry name" value="SDR_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000084">
        <property type="entry name" value="Short-chain dehydrogenase reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020904">
        <property type="entry name" value="Sc_DH/Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002347">
        <property type="entry name" value="SDR_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43180">
        <property type="entry name" value="3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43180:SF66">
        <property type="entry name" value="SHORT-CHAIN DEHYDROGENASE_REDUCTASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13561">
        <property type="entry name" value="adh_short_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00081">
        <property type="entry name" value="GDHRDH"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00080">
        <property type="entry name" value="SDRFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00061">
        <property type="entry name" value="ADH_SHORT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0521">NADP</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP77092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="292" mass="30709" checksum="1314F1004A225067" modified="2011-01-11" version="1">MSNQQPSTSSPNDRLQQIVNHFDSRNSSKKLSGKVCILTGIGHELGIGWATAVLFAKEDAKHLYLLDLKGDKFPALVKHLKELNPKIGLSTISGDAAEESVVRSVCQKAIDEQGRLDVFFANAGVVGAAHIGKTSYETSERVIRINTMSCWLALKYASDAMLKAGKGGSIIFTSSAAGLRSGAGSTEYSASKAAVISLAATGANAYPGAAIRVNAVCPGMIQTQMTGPVFQMAKDKSKIGQLCPLRRYAVPDEVASVVLFLASDDSSYVTGTAIPVDGGLASSLPVIPGRQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3JYJ2</accession>
    <name>E3JYJ2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">HIT-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_03073</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77117.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321536.2">
        <property type="nucleotide sequence ID" value="XM_003321488.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JYJ2"/>
    <dbReference type="STRING" id="418459.E3JYJ2"/>
    <dbReference type="GeneID" id="10537328"/>
    <dbReference type="KEGG" id="pgr:PGTG_03073"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03073"/>
    <dbReference type="HOGENOM" id="CLU_2334666_0_0_1"/>
    <dbReference type="InParanoid" id="E3JYJ2"/>
    <dbReference type="OrthoDB" id="18412at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23024">
        <property type="entry name" value="zf-HIT_ZNHIT2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007529">
        <property type="entry name" value="Znf_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039646">
        <property type="entry name" value="ZNHIT2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15555">
        <property type="entry name" value="ZINC FINGER HIT DOMAIN CONTAINING PROTEIN 2 PROTEIN FON -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15555:SF0">
        <property type="entry name" value="ZINC FINGER HIT DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04438">
        <property type="entry name" value="zf-HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144232">
        <property type="entry name" value="HIT/MYND zinc finger-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51083">
        <property type="entry name" value="ZF_HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="HIT-type" evidence="2">
        <location>
            <begin position="29"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00453"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51083"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP77117.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="98" mass="11232" checksum="805A87268D4EF52E" modified="2012-04-18" version="2">MDSPILQPLKPAGKRRRRINQANQDTIRCFICQEKFAKYTCPSCNLRYCSVDCFKSQSHSACSESFYKNALLEDFQLEDGSSNGSALNLLRQRRCSRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZ37</accession>
    <name>E3JZ37_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_03268</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77312.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321731.2">
        <property type="nucleotide sequence ID" value="XM_003321683.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZ37"/>
    <dbReference type="GeneID" id="10537858"/>
    <dbReference type="KEGG" id="pgr:PGTG_03268"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03268"/>
    <dbReference type="HOGENOM" id="CLU_1866149_0_0_1"/>
    <dbReference type="InParanoid" id="E3JZ37"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP77312.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="137" mass="15014" checksum="818704AF98D5DD27" modified="2012-04-18" version="2">MTRGRVYWSQGAVVGCTRHEMPFLQCHELECPQVNGQSITRETINITGVGTIVAKIDRQGGFLDLFVKHDVGIRTAFGDKVITVKYVVNKSQCGEGVSLLLKPGQRGKFMGALAGWDRNNCTMVVDMRGATVGQNNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>E3K0K6</accession>
    <name>E3K0K6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Pectinesterase</fullName>
            <ecNumber evidence="4 11">3.1.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_03787</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in maceration and soft-rotting of plant tissue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 11">
            <text>[(1-&gt;4)-alpha-D-galacturonosyl methyl ester](n) + n H2O = [(1-&gt;4)-alpha-D-galacturonosyl](n) + n methanol + n H(+)</text>
            <dbReference type="Rhea" id="RHEA:22380"/>
            <dbReference type="Rhea" id="RHEA-COMP:14570"/>
            <dbReference type="Rhea" id="RHEA-COMP:14573"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:140522"/>
            <dbReference type="ChEBI" id="CHEBI:140523"/>
            <dbReference type="EC" id="3.1.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the pectinesterase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.11" evidence="4 11"/>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77831.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322250.2">
        <property type="nucleotide sequence ID" value="XM_003322202.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K0K6"/>
    <dbReference type="GeneID" id="10540815"/>
    <dbReference type="KEGG" id="pgr:PGTG_03787"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03787"/>
    <dbReference type="eggNOG" id="ENOG502QSQ4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_012243_1_0_1"/>
    <dbReference type="InParanoid" id="E3K0K6"/>
    <dbReference type="OrthoDB" id="2498421at2759"/>
    <dbReference type="UniPathway" id="UPA00545">
        <property type="reaction ID" value="UER00823"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030599">
        <property type="term" value="F:pectinesterase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042545">
        <property type="term" value="P:cell wall modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045490">
        <property type="term" value="P:pectin catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.160.20.10:FF:000014">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.10">
        <property type="entry name" value="Single-stranded right-handed beta-helix, Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012334">
        <property type="entry name" value="Pectin_lyas_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011050">
        <property type="entry name" value="Pectin_lyase_fold/virulence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033131">
        <property type="entry name" value="Pectinesterase_Asp_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000070">
        <property type="entry name" value="Pectinesterase_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31321">
        <property type="entry name" value="ACYL-COA THIOESTER HYDROLASE YBHC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31321:SF57">
        <property type="entry name" value="PECTINESTERASE 53-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01095">
        <property type="entry name" value="Pectinesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51126">
        <property type="entry name" value="Pectin lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00503">
        <property type="entry name" value="PECTINESTERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0063">Aspartyl esterase</keyword>
    <keyword evidence="11" id="KW-0961">Cell wall biogenesis/degradation</keyword>
    <keyword evidence="7 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0964">Secreted</keyword>
    <keyword evidence="6 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005127844" description="Pectinesterase" evidence="11">
        <location>
            <begin position="26"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Pectinesterase catalytic" evidence="12">
        <location>
            <begin position="39"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="active site" evidence="10">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP77831.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="326" mass="34452" checksum="6BA107952411117B" modified="2012-04-18" version="2">MVSHKQVRQNLFIAAFFSLSAWAQSSPPTGALIVRQGSSTGRGEFSTISAAVAALARLTGPQTIFIYPGTYTEKVKITYPYSLSVQGYSANPTSTASNQVTVNAAVSAAQAGSNSNSATIWAHSSGIRISNLNIINSFGTGRDTQALALASTGNRQVFKYCAFSSFQDTVEIEGTAYFYGSHIEGAVDFIFGPGSAWFESVVLGVKASPTPVITAQRNSPGGGTAFVFNHAQIVSAGAKPGSAYLGRPWSPDASVVYQFCSLSNVINPQGWKIWSPSQPNTDRVNFQEFQNVGPGASTSARQIGTQRSAQVNIASILGSDYSTWAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3K196</accession>
    <name>E3K196_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">C3HC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_04027</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP78071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322490.1">
        <property type="nucleotide sequence ID" value="XM_003322442.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K196"/>
    <dbReference type="STRING" id="418459.E3K196"/>
    <dbReference type="GeneID" id="10541541"/>
    <dbReference type="KEGG" id="pgr:PGTG_04027"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04027"/>
    <dbReference type="eggNOG" id="KOG4765">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056223_0_0_1"/>
    <dbReference type="InParanoid" id="E3K196"/>
    <dbReference type="OMA" id="HLDYCPW"/>
    <dbReference type="OrthoDB" id="2592092at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012935">
        <property type="entry name" value="NuBaID_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15835">
        <property type="entry name" value="NUCLEAR-INTERACTING PARTNER OF ALK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15835:SF6">
        <property type="entry name" value="ZINC FINGER C3HC-TYPE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07967">
        <property type="entry name" value="zf-C3HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C3HC-type" evidence="4">
        <location>
            <begin position="108"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="77"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="298"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="432"/>
            <end position="457"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="49"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="77"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="309"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="446"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP78071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="484" mass="52589" checksum="7EA61E461C83CCE0" modified="2011-01-11" version="1">MTTMPSSTSTTSLQAVSIDPSRLKNAFDILNQIDCLQQNDIRTAPNSQSITSSPKKNISSSSNSRIISTPALEAIRSSRINGRSKSSSSKRVKTELRTKLNPQGKVNPGSQIDFLGRLETFKLSSYPAGKPRGLSPPSMAAHGWTNAQKNRLKCVCCGATWVLATPSKGDWGSSSGAQLVTLGIRMRTEQHRSSCPWRSRSCPPSIYHTPRWKSSRETFEALIKVAVEISDALFSNARTVFQVQHNLPDRVVTTLSIALQTENSSGSTLPSRSCPTTDALILALFGWSIKRVPAGRRDSSQSMAALPSIQGSNPASGRSTTLPESDDLPSLHCRLCHRQALPSPTSTKAFNPVHEHREYCPFIDPHAGFDPPQGPSAAADSRPGWHARLEAIQNVLDRQNFSICPSSSSSDSPHPLPNQSYVTLENIARHFNTRPPVTPSDHPLGSENTHTPVNPSRDSCPQINLLNFVKEVLVGPPSQLLSSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="75" xmlns="https://uniprot.org/uniprot">
    <accession>E3K1K4</accession>
    <name>E3K1K4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_04135</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP78179.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322598.2">
        <property type="nucleotide sequence ID" value="XM_003322550.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K1K4"/>
    <dbReference type="FunCoup" id="E3K1K4">
        <property type="interactions" value="178"/>
    </dbReference>
    <dbReference type="GeneID" id="10541809"/>
    <dbReference type="KEGG" id="pgr:PGTG_04135"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04135"/>
    <dbReference type="HOGENOM" id="CLU_339518_0_0_1"/>
    <dbReference type="InParanoid" id="E3K1K4"/>
    <dbReference type="OrthoDB" id="10266696at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08204">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.220.150:FF:000009">
        <property type="entry name" value="stromal membrane-associated protein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.220.150">
        <property type="entry name" value="Arf GTPase activating protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.29.30">
        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051718">
        <property type="entry name" value="ARF_GTPase-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037278">
        <property type="entry name" value="ARFGAP/RecO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001164">
        <property type="entry name" value="ArfGAP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038508">
        <property type="entry name" value="ArfGAP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011993">
        <property type="entry name" value="PH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001849">
        <property type="entry name" value="PH_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705">
        <property type="entry name" value="FI20236P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45705:SF1">
        <property type="entry name" value="FI20236P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01412">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00169">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00405">
        <property type="entry name" value="REVINTRACTNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00105">
        <property type="entry name" value="ArfGap"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00233">
        <property type="entry name" value="PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57863">
        <property type="entry name" value="ArfGap/RecO-like zinc finger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50729">
        <property type="entry name" value="PH domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50115">
        <property type="entry name" value="ARFGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50003">
        <property type="entry name" value="PH_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0343">GTPase activation</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="PH" evidence="7">
        <location>
            <begin position="503"/>
            <end position="599"/>
        </location>
    </feature>
    <feature type="domain" description="Arf-GAP" evidence="8">
        <location>
            <begin position="826"/>
            <end position="940"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="50"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="486"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="688"/>
            <end position="823"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="9"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="744"/>
            <end position="767"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="768"/>
            <end position="823"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP78179.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="940" mass="107238" checksum="4F761C28D5AF1E19" modified="2012-04-18" version="2">MEQQQQQQLINKLSTPSLSTNNQNNNHSKRLQISSLYNQHKHRLHSLLPKPQPTHIQQQPPPPPQQQEPSTNRLSPEPFDSLINKHFTSSILPGLSSSAFPSSSATDPIIPDAQLEDGPLLRAQLSGLEHRASLLKKSIKPLIKVFELIHQNLCTSLESDTRLDQALEILDETTPACFRPLKEIYYKSAKLQNRAIDLERKERLELQILDPLRRMSSNLKEINQKKKSFIHQAKLYDETLQKFLSIKSEGEAEMKKMEQMKAKVEFARIEYHHWIYEQAVFQEVEVLELLTEYMFSEHACRRSPTSSETDTIGIELIHLRQELAIRHIERETQRRAMENHRQAWRNKLDSFLQSSTTADHHARLLNTSDTPPKAKLMIRTTSAFDHLHSNPSSHRTHELGEKLRGFALTLTGKAFGSEEGITSSSPRGRKNLAIPNSLAMAISPSIESDEQFEDHSIKRMRSWSANVQRKKEGFLLSCTKSLAAGGPVQRHHHHNQQHHPTEQSQGTGGIPNGSDGVRSWKKLWCVLAGGELREYKGDELVDPHRSGIDLRYAMVRPRKGKAERKFSFEVVTTKFVRVYQAFSLLEMHEWVAAIEGSIESILNSSTSAKTARPKPGGEQARIATLTIPKSKPVISSIAVRRLESDYPAEGVIGKTKAVSSNTRKKRANTVAHQEGGLQVEWKPIGDSSSLLHHRSAPSDLPHHLPPPARLWSPLPSDQDDHSPRSRSLTVEEDLESSQPSVPVRPDDQARAKRRMIEEDDDNRRSVTDEGDEEDEEEDEEEEEGDLGDEEEDEEEEEEEEEEEEEEEEEEEEQPQEDEEEEGTMYERNWTELDNLSHRSSCAECGKPNPRWASYSLGILICINCCGIHRGMGTHISKVRSLDLDDWNNEQIRQIRVVGNQKSKAFWEASLPKDFHLTPSNIKQFVHDKYIHKKFIPKNTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3K1V0</accession>
    <name>E3K1V0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_04231</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP78275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003322646.1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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    <proteinExistence type="predicted"/>
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        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172308" evidence="2">
        <location>
            <begin position="21"/>
            <end position="876"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="42"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="79"/>
            <end position="106"/>
        </location>
    </feature>
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            <begin position="148"/>
            <end position="175"/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="157"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP78275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
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</entry>
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    <accession>E3K2U1</accession>
    <name>E3K2U1_PUCGT</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
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    <dbReference type="KEGG" id="pgr:PGTG_04616"/>
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    <dbReference type="HOGENOM" id="CLU_1090456_0_0_1"/>
    <dbReference type="InParanoid" id="E3K2U1"/>
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    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="41"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78660.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="255" mass="27771" checksum="8046B523450798C8" modified="2012-04-18" version="2">MSTLERLDLYEKVQALAAHPALKPNEWGHIQALLNDIKADYENSDDEDNTGGPEVTNIQRSPATPVGEERPQTAVHSFNLPAAKSPAGPTGSESYLAPEPQAAKDNRTPNQSPSRAPRIGEFFILPNPIGAPRTTCPTFSQFLSGNTTTAFTSLHKGPHMTLSNANALLELGSSVLKQKIALGITTPMPHSTLDNLRIHFLNFLIKMGFNQLDNNGVPQVNWKDVHASFYNEFSPSVIAGIFSQDIEIALAHRLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K353</accession>
    <name>E3K353_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_04866</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
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            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78910.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003323281.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K353"/>
    <dbReference type="GeneID" id="10537098"/>
    <dbReference type="KEGG" id="pgr:PGTG_04866"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04866"/>
    <dbReference type="HOGENOM" id="CLU_787871_0_0_1"/>
    <dbReference type="InParanoid" id="E3K353"/>
    <dbReference type="OrthoDB" id="10324336at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="chain" id="PRO_5003173198" evidence="2">
        <location>
            <begin position="20"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="162"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP78910.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="352" mass="39392" checksum="6D154DCDD2A262BB" modified="2012-04-18" version="2">MKNPSRCIFLALVFTRCKGTLDRFPFDWTIGAGDVLLPNPGVENQRQSWDALGSFSGNPDYLSSLPLAISRVEPVNTAGGVPVTMTDSRVSNPHLENTYETRERPTTHANGPLADPEYNNDISIQQFPVGLAPPSTHMMRSPISISSFPGNLHYTYASQELSKTSTGGDLTTRGQVNDQDTLPKDPYEAQEPAIPFTPMFSWHGLSIPDIPIEVYEKRFINELRSKRARDLVVHSTSGLLPGNKRQAKFEGYFKGKHAMYRQDITSDWFNAPNMITTIFPKPGNVDVTVDKHQLFESAFKIFELRAPQIPNAARTKYPSFIWECVDHWLCALFSHSVTTLSEKAAGKKVLME</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3B3</accession>
    <name>E3K3B3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Cyclin N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_04926</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78970.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323389.2">
        <property type="nucleotide sequence ID" value="XM_003323341.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3B3"/>
    <dbReference type="STRING" id="418459.E3K3B3"/>
    <dbReference type="GeneID" id="10537158"/>
    <dbReference type="KEGG" id="pgr:PGTG_04926"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04926"/>
    <dbReference type="eggNOG" id="KOG1674">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018149_2_1_1"/>
    <dbReference type="InParanoid" id="E3K3B3"/>
    <dbReference type="OrthoDB" id="10250320at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000307">
        <property type="term" value="C:cyclin-dependent protein kinase holoenzyme complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016538">
        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019901">
        <property type="term" value="F:protein kinase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20557">
        <property type="entry name" value="CYCLIN_ScPCL1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013922">
        <property type="entry name" value="Cyclin_PHO80-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15615:SF10">
        <property type="entry name" value="PHO85 CYCLIN-2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin N-terminal" evidence="2">
        <location>
            <begin position="114"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="247"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="313"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="367"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="321"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="377"/>
            <end position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP78970.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="387" mass="42768" checksum="31E8A356D89C4A88" modified="2012-04-18" version="2">MIFNKATSLFPTPVKPTAAISTYPPLVRRHPASLIAKSLHHPGLLALVRQRVSREMISHIAQVAISVIQCQSDSPESLLSPPATPIRLEHVGRDQTPSRTPSPYSRDPRKEAESSLPSLEAFIQIVVEKSNVQVPTLLCTIIYLERLRTKLPKIAKGLNCTRHRVFLAALIVAAKYLNDSCPKNKHWCSHASIFADVEVNLMERQLLFLLDFDLRMDEETLLKAFAPFLPDASPRAIPAHLSQKVLPPTPYRAPSNQSFANTPPSISHTTPRNNLTKYRDSHHGAPLTPSPSPIRRLMAYGNGLADKTIQRIGGAQRGPKASPASSATSSNPSTVDNSSPEEELLFQLSLSGHKPFRALQAPHKIQLERSPFSSHHPVTRRNVSHSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3D9</accession>
    <name>E3K3D9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_04952</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78996.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323415.2">
        <property type="nucleotide sequence ID" value="XM_003323367.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3D9"/>
    <dbReference type="GeneID" id="10530817"/>
    <dbReference type="KEGG" id="pgr:PGTG_04952"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04952"/>
    <dbReference type="eggNOG" id="KOG0637">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019945_0_0_1"/>
    <dbReference type="InParanoid" id="E3K3D9"/>
    <dbReference type="OrthoDB" id="28755at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008506">
        <property type="term" value="F:sucrose:proton symporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19432:SF35">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 45 MEMBER 3 ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19432">
        <property type="entry name" value="SUGAR TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="180"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="216"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="256"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="292"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="375"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="494"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="539"/>
            <end position="558"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="564"/>
            <end position="590"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="663"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="731"/>
            <end position="751"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="88"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="20"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="45"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="88"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="116"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP78996.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="783" mass="88404" checksum="E8647F6E4C63DCAB" modified="2012-04-18" version="2">MSFSDSLKFQETVNKEENQPRTTTTKMKSPNGSNPRTTYHYTLNQQQQQQQQQQQQQQQQQQQSRPFLLNRATNEITQFPSYETMYQLSASPTTRNNQQQQQQQHQPIPSPSHNFSSSTIQSITQSTSSSNNHQQQENQSSFTHFLRRLLNKQHQKLITNQPITTEPSPEELLDHSPAKLIPLTLLLFASQLISIITTFYAIPFLIKLRPSNLSRFILWIAFPLAGLIIPVYLGTSSDLRTQSKHRRTAPIAYSTPILICSLLSLSFCQPIGTLIIYILGLDQGDWDPNRKIYIQSASIAIGILSLYPLTFSLNALIQSARSLILDQIHAENQPLVNIWITRLTRLADLLVFLIVSGSSDADLHGPNQPNLLRKLVGFSIPVLLLCSLITCSHVIERPAFENHSSSLTPNSSIRTILKAQFNLFRQTLIDLPIPIRRVCYVEVLSSTCWLTIFYHAKPLVTRFTLVELSSKGAKLTESLVRYAERQGTKAMLNFSIVSLITALILPSLATIATTEYFITRRGKAWNRLRRIVAYVTPRNLWTFGLVLYAILMCSTFSIETGAGANLLISFLGLSWTLAQWVPLSLVMEYIRSIEETTMVRLEIDEAGRSNSPVPSSPQLRMPTAKHEPRLSEQSPLLSSAPDPPSLVSLSASHQYATVRGGTYLAIFNLASVVPHFLITIITSTMLTIVQLIESNEEALNSIDTRSHDHPFCPSFWSRDSSVTEVPLKTLWLFRLTGLVALLAVFLSRSLVVPTSELDYWDEIKFQIFEDRADRLLVNQVEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3J8</accession>
    <name>E3K3J8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Phosphoacetylglucosamine mutase</fullName>
            <shortName evidence="12">PAGM</shortName>
            <ecNumber evidence="4 12">5.4.2.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">Acetylglucosamine phosphomutase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10 12">N-acetylglucosamine-phosphate mutase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PGTG_05011</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the conversion of GlcNAc-6-P into GlcNAc-1-P during the synthesis of uridine diphosphate/UDP-GlcNAc, which is a biosynthetic precursor of chitin and also supplies the amino sugars for N-linked oligosaccharides of glycoproteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 12">
            <text>N-acetyl-alpha-D-glucosamine 1-phosphate = N-acetyl-D-glucosamine 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23804"/>
            <dbReference type="ChEBI" id="CHEBI:57513"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="EC" id="5.4.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="12 15">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route I): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.3" evidence="4 12"/>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP79055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323474.1">
        <property type="nucleotide sequence ID" value="XM_003323426.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3J8"/>
    <dbReference type="FunCoup" id="E3K3J8">
        <property type="interactions" value="27"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K3J8"/>
    <dbReference type="GeneID" id="10530876"/>
    <dbReference type="KEGG" id="pgr:PGTG_05011"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05011"/>
    <dbReference type="eggNOG" id="KOG2537">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022890_1_0_1"/>
    <dbReference type="InParanoid" id="E3K3J8"/>
    <dbReference type="OMA" id="WEAYATK"/>
    <dbReference type="OrthoDB" id="1928at2759"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00530"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004610">
        <property type="term" value="F:phosphoacetylglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03086">
        <property type="entry name" value="PGM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.50:FF:000003">
        <property type="entry name" value="Phosphoacetylglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000023">
        <property type="entry name" value="Phosphoacetylglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000038">
        <property type="entry name" value="Phosphoacetylglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049023">
        <property type="entry name" value="AMG1_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049022">
        <property type="entry name" value="AMG1_III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016657">
        <property type="entry name" value="PAGM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45955">
        <property type="entry name" value="PHOSPHOACETYLGLUCOSAMINE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45955:SF1">
        <property type="entry name" value="PHOSPHOACETYLGLUCOSAMINE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21405">
        <property type="entry name" value="AMG1_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21404">
        <property type="entry name" value="AMG1_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016408">
        <property type="entry name" value="PAGM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7 12 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 12 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="17">
        <location>
            <begin position="57"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoacetylglucosamine mutase AMG1" evidence="19">
        <location>
            <begin position="183"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoacetylglucosamine mutase AMG1" evidence="18">
        <location>
            <begin position="297"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase C-terminal" evidence="16">
        <location>
            <begin position="443"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="13">
        <location>
            <position position="65"/>
        </location>
    </feature>
    <feature type="binding site" description="via phosphate group" evidence="15">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="276"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="280"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="374"/>
            <end position="376"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="488"/>
            <end position="492"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="497"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00032065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR016408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR016408-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR016408-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR016408-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF21404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF21405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="EFP79055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="533" mass="57872" checksum="0CB5AF90681BFA36" modified="2011-01-11" version="1">MDAKQIEAVAASYPSPPGIKHTFGTAGFRANADTLDRVFFTVGLLAVLRSKKLAGQTIGVMVTASHNPAQDNGIKLVDPQGEMLASSWESHATVLANSADLPSSLTQIIDSETIDFKQPAKLILGHDTRPSCARLIKAFKDAVKALDPSAQILESGLKTTPQLHYLVKCINDGGVYGAPTEEGYYKKLSDAFIALNAETKTPYTLMVDCANGVGGPKLESMQAYLKDSPLRLKLSSTDVNSSEKLNKDCGADYVKTTQSAPSGVSLEPLARACSFDGDADRIVYYYYSEKKSFRLMDGDKIATLVASYIKQLVELESAEIKDHLRIGVVQTAYANGNSTRYIKDQLGLAVTCTPTGVKHLHHAAQTFDIGTYFEANGHGTVVFSEALLKRLPASSKLLSLAGLINQTVGDSMSDMLLVETILNARGWGMAEWDAKYEELPNRLVKVIVSDRHAFVTEDAERVLVKPDGMQLKINQIVANYPSARSFVRPSGTEDCVRVYAEAGTKEATDELAYKVAGMVFEFCGKGEKPKEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3K5W2</accession>
    <name>E3K5W2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6589 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_05854</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323952.1">
        <property type="nucleotide sequence ID" value="XM_003323904.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K5W2"/>
    <dbReference type="GeneID" id="10531361"/>
    <dbReference type="KEGG" id="pgr:PGTG_05854"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05854"/>
    <dbReference type="HOGENOM" id="CLU_009176_0_0_1"/>
    <dbReference type="InParanoid" id="E3K5W2"/>
    <dbReference type="OrthoDB" id="3207600at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046496">
        <property type="entry name" value="DUF6589"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20231">
        <property type="entry name" value="DUF6589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6589" evidence="2">
        <location>
            <begin position="388"/>
            <end position="680"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="187"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP79533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="685" mass="76485" checksum="6208D8454F58DEE1" modified="2011-01-11" version="1">MLVRILEFERPNVNDHPIRSRHLSGSAMNSTEETTPAGRAPRVSMNEKLDKICALIEELNLTPKSFLVAFLEQDQYSMAYRRRLWGTDQGWDSTENLLLTIRKLACSHLDGRGHWERFILMQAINIVSAQKPVSGLAPKGSYHTSATLSDVFFTTEEREARNDLLTDRMPFLYQLLCAKIQGDRPYTTTGGAADSPADPVEDNSDDEGVDDPSDGLVDYDGSVLKKSNERGIRRANWVKTMARTICAMVAFGGNRRHNGFQLSNALLFLAGGVTERVSAYLNYIGISSSWRTAHAALRSLGSEAKSKLEAIYKMGDSPLVAPLLCYDNLDFQEKVHMKSIGHSSKMFHGTWGYVHTIPRSLQSQLDPAELTVEALNKALHIGKDLKIRPDMFTPTAESTIHFETTIKSQITRVILKYFATPTDKRVDLLVKPLEVNPIAPDDPNITMLKLMVASDNSALGVGEVFTGVIQQSGLTPAEFHSRLQIVEGDLGSCNIFDSLRKQRTPALGNHNSLDNILPIPGAAHTLWNLSQAIYLAHWGNEKLARDTGAWRTLHALGIPAEKPVTKKDFNLMLCHIEKIHEATLLYCVLLVANRAHEPWSGKRLKVSTESIHDWVELTFDRFCSGAALQTELASNYSGHKNLLLRIRDFGTIVEANRAMKDGDYGRLMFMWQRWAVMSQGICQPT</sequence>
</entry>
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    <accession>E3K7B7</accession>
    <name>E3K7B7_PUCGT</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80447.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324866.2">
        <property type="nucleotide sequence ID" value="XM_003324818.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K7B7"/>
    <dbReference type="STRING" id="418459.E3K7B7"/>
    <dbReference type="GeneID" id="10539456"/>
    <dbReference type="KEGG" id="pgr:PGTG_06403"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06403"/>
    <dbReference type="HOGENOM" id="CLU_004591_5_1_1"/>
    <dbReference type="InParanoid" id="E3K7B7"/>
    <dbReference type="OrthoDB" id="2288618at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP80447.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="518" mass="59288" checksum="AF152479DF48618E" modified="2012-04-18" version="2">MFALDARQIIGDDLMNPLVAPHLDFLPEDAQGECVFKTSQSAKWLKHLDPDLRVQMVFYNLKHYYIYEPVQLKNDEIVIPIFFYMLGSKTLSKCYKPKIKSKKIPIATNLTYDDPCLQTVPLDDFDLLYSEIVSSDGLRLSKICGDVLFKQDSEDTIKLPNPWRTKANGRIIRHVPVNMYCDDTSGNKSKKWNKHISYYFTLSGLPPKISNQQFNCHFLCTSNIAGALDLGEIVVQQLNEMMNTGYEAYDMMLSQTVLVMTPMLCLLADSPMHAELTNTPVPGTSLNPCRVCVLSSKSLQDKKTLSFLSKFQPNHLRSTEKTIQDSKEVWRYVKENARTINKEKLDEKSAEFGIRDQRNRKFAKEIIAFREKKKALLKDGRELPEDMEQAIPQGVMDLESKDESQMLSPFLKLKGMCCFDMVKDTPVEILHVFLLGIVKYLFADFMSGLDEEQKKELEALWSSFNTSSLNIPSIRPTSMILFSDMVLPVFLPQKNLRVIMVFLEMLQFTPTVTALAKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3K8N4</accession>
    <name>E3K8N4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06486</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80530.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324949.2">
        <property type="nucleotide sequence ID" value="XM_003324901.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K8N4"/>
    <dbReference type="GeneID" id="10539541"/>
    <dbReference type="KEGG" id="pgr:PGTG_06486"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06486"/>
    <dbReference type="HOGENOM" id="CLU_124571_0_0_1"/>
    <dbReference type="InParanoid" id="E3K8N4"/>
    <dbReference type="OrthoDB" id="2504124at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171815" evidence="1">
        <location>
            <begin position="19"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP80530.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="162" mass="17600" checksum="5E8642DEF70B8F6B" modified="2012-04-18" version="2">MHLASFIILSLSFYYAQADSNTSMSGPQLKPMKCTNIYLTLSERDIAEMQSKVSTNPVKAVCKSSAAGDNGGLCDFKTCSGKPAVCGTCYPVTMKEGKLVRTSETSVEKVECGKNYFLKTEKDHNICTAYDNKMYSCTGECKASIECQSCVSLDDPAFKKAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3K946</accession>
    <name>E3K946_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphatidylinositol-specific phospholipase C X domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_06638</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325101.1">
        <property type="nucleotide sequence ID" value="XM_003325053.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K946"/>
    <dbReference type="STRING" id="418459.E3K946"/>
    <dbReference type="GeneID" id="10539699"/>
    <dbReference type="KEGG" id="pgr:PGTG_06638"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06638"/>
    <dbReference type="HOGENOM" id="CLU_474180_0_0_1"/>
    <dbReference type="InParanoid" id="E3K946"/>
    <dbReference type="OMA" id="GIVMFDF"/>
    <dbReference type="OrthoDB" id="1046782at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.190">
        <property type="entry name" value="Phosphatidylinositol (PI) phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051057">
        <property type="entry name" value="PI-PLC_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017946">
        <property type="entry name" value="PLC-like_Pdiesterase_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13593:SF116">
        <property type="entry name" value="PLC-LIKE PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51695">
        <property type="entry name" value="PLC-like phosphodiesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50007">
        <property type="entry name" value="PIPLC_X_DOMAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="149"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="564"/>
            <end position="589"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="151"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP80682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="589" mass="64857" checksum="391D075F312D2ECD" modified="2011-01-11" version="1">MSDQLPPGPPWHHPPHQNQPSGSHIIHIISSSSTLQEEEEEGADIRLSKSYPFCADPLIEKKKKKNEQQQSENAGEMMMSVLMTTIRGRRVQWLCNWLGSLVLAAILILSLSRPLGRASPFLPPNTPSPASSAPQQLNPDVRFQIGFQTHSSSSSSPASDPDLSSSTAPTNQIAPPLTSPPLVFSHDQADEIARRIFNAAPVLGYYEQSNGRLSNWMANVADGVAITSMSIPGTHDAATWNYTQATQDYLEPVTGKLPPAIAFQCQDRSLFQMLGDGIRFFDLRVGFLPDHQQLGFYHASALLSTTATLPDVLLGFYKWLDEHPTETVLMSIKVDNATFGNPPSPGQPSSKTLQLMLYQLLTQSQLARDHWLQEDSKLGTLGEARGKLIFIQRIDWSEIRSRREDYTPIGIPLPPQQFNDNDPDFSILYNPVDRASAYVEDFYNILPNPSPILDKFNRKFDAVQNHLSLAAALNPQTVDQLFITFASGGALLNSPPVTPKMLAIGTGEGASLKNSVNGRIEALIQEQYSNGANDPNNNGQAPQRLGILIFDWYHQLPNLVQSVINQNPFSPPPPSSSPSSTDDYSNYGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>E3K9T2</accession>
    <name>E3K9T2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ysc84 actin-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_07197</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP80945.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325364.2">
        <property type="nucleotide sequence ID" value="XM_003325316.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K9T2"/>
    <dbReference type="STRING" id="418459.E3K9T2"/>
    <dbReference type="GeneID" id="10536103"/>
    <dbReference type="KEGG" id="pgr:PGTG_07197"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07197"/>
    <dbReference type="HOGENOM" id="CLU_035531_0_0_1"/>
    <dbReference type="InParanoid" id="E3K9T2"/>
    <dbReference type="OrthoDB" id="10255128at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11524">
        <property type="entry name" value="SYLF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051702">
        <property type="entry name" value="SH3_domain_YSC84-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007461">
        <property type="entry name" value="Ysc84_actin-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15629:SF8">
        <property type="entry name" value="DUF500 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_5G07310)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15629">
        <property type="entry name" value="SH3YL1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04366">
        <property type="entry name" value="Ysc84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ysc84 actin-binding" evidence="2">
        <location>
            <begin position="195"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="328"/>
            <end position="561"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="16"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="360"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="428"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="461"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="489"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="501"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="521"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="547"/>
            <end position="561"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP80945.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="561" mass="59655" checksum="3038709CF56DFDF8" modified="2012-04-18" version="2">MTTKPRPQSAFQLNHSSSSTTTTKTKTKTTTTATNQASSDEGQDGDHELRRQQTLKTTLSGTAKTSLDWLWQKSGILGNWINIKHTGNIGIQAFWPTSLDKECDKCARILYTFTQRGLNGTVHSTLDDTQADKRKTGEGVTKKIPTHIIQQAEGLAIFTVFRSTHAAPSASGSGLLISRDSPTTWGAPSGILIHSLDSGFLAGIDLYDVVLVLRTQKAVMSFAKPKVSLGPELAVVAGPIGNGVSFESGPEISPVFSYTKSKGIYGGLQLDGNIIEERTDENARFYGRKVKAEQILQGGAVAVPRAASGLIAVIDAAEGKAYDPEDFPASIDVAPSEESMGVSTIRESRAKAAQDSGIVEMDEEADEEQQEEEETEVREPTKPDASSPRRRTMPPMAFHPASGSPGKATKQEPQPEPDGAVEADKESAPLAALTLVAPLAAPPPPPPRRRGLTVSRPPTEEGSTGPHSPTTLVPTRQDLPHQASQETIHPPPPPPPPPSAPDLDSLSISDSVALLSNNSDKPPEPFLLHERSDSSHSITSPPKPSETEPSSSSSLASVPLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAC4</accession>
    <name>E3KAC4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06902</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81281.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325700.2">
        <property type="nucleotide sequence ID" value="XM_003325652.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KAC4"/>
    <dbReference type="GeneID" id="10536769"/>
    <dbReference type="KEGG" id="pgr:PGTG_06902"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06902"/>
    <dbReference type="HOGENOM" id="CLU_109037_0_0_1"/>
    <dbReference type="InParanoid" id="E3KAC4"/>
    <dbReference type="OrthoDB" id="10301853at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="45"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81281.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="181" mass="20183" checksum="0863B81926C7D108" modified="2012-04-18" version="2">MGLINLTTPDPKLSLEHPTALSGKTSASHPSSSQTPILPISHPDGSTQSSTGGEPSSSYSGLANQAVQQYNPLRIAQINQPAEMAENNIPALIDDVNHVHLDNGLINLQDEHQLLPDLWDQDRLLFWDNLNQFGGNLLVIFAWDLFAKAFQNYLDFEIGLLIRLAFFLFNIFDMLGEYFFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3KB68</accession>
    <name>E3KB68_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycogenin glucosyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81581.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326000.2">
        <property type="nucleotide sequence ID" value="XM_003325952.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KB68"/>
    <dbReference type="STRING" id="418459.E3KB68"/>
    <dbReference type="GeneID" id="10532962"/>
    <dbReference type="KEGG" id="pgr:PGTG_07830"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07830"/>
    <dbReference type="eggNOG" id="KOG1950">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_340677_0_0_1"/>
    <dbReference type="InParanoid" id="E3KB68"/>
    <dbReference type="OrthoDB" id="2014201at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02537">
        <property type="entry name" value="GT8_Glycogenin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000193">
        <property type="entry name" value="Glycogenin glucosyltransferase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002495">
        <property type="entry name" value="Glyco_trans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050587">
        <property type="entry name" value="GNT1/Glycosyltrans_8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11183">
        <property type="entry name" value="GLYCOGENIN SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01501">
        <property type="entry name" value="Glyco_transf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="369"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="483"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="537"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="712"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="386"/>
            <end position="396"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="486"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="567"/>
            <end position="601"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="630"/>
            <end position="643"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="645"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="669"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="714"/>
            <end position="724"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="730"/>
            <end position="742"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81581.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="742" mass="83221" checksum="055B9FBAD4CE61E9" modified="2012-04-18" version="2">MASWAFVTVLTSDSYLPGALVTARSIKDVEKASTRQFDLVCVVTLDSVSVQSIKALRQMYDLVISAEVIRSGHSEHELNLLGRQDLSSTITKIHIWRLTQYEKVIYVDSDTLLLRPLSHLFELASPFSACADIGWPDCFNSGLMVIKPSNETFEKIFQHFLTHGSWDGGDQGLLNDYFAQSSGELSPAGSDGQSQGWNRLSFVYNVTPSTYYTYAPAYKRYGDKISMIHFIGSDKPWHLINRRRYRNAYAPNQNSESLNAVDYDSLVDHWLDVYETVVGPIEPLEWSSFQPEFNVPKYLSEWDSTQPSAYKPPSLDELKETFSRRRAVIGPQSFGMKAPNDEGGYMTLPFLDLSRLAREYILARDKATTALDSTRDSRSVPPESNHLQERRDHLHQVWDPSRSSPPPSGGYQMNEPITKHYEAVWDKPFSEQSRSFFQPPTTTHSIPHITHQDYSQFSRPPQPNAVQAVFPWEENARHHAGRVFPSGESRTSTSGSSHHQDHNHSEANANMVNQASLSQIYRNAWDDDPMIGRWAKARSVSGSNKSPSPGWAKSEKTKAALVQTPRLEIRGFDFDRRGTEGKKAGRETSDVGRIPTFDHSHQHQHHPSRQITFSSSLYRPRSDSEGSSQDADEEDVGEDEDQSSDVRALTEGTSSELTSTTPPEKSKASDQISGTLEATENPEVNKTGSFPSTFRLPTNRSMIIGVPVQMLKTSSSSSNSSAHSWEGDNNKNLARRTTSLMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KC79</accession>
    <name>E3KC79_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">F5/8 type C domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_08264</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EFP82015.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326434.2">
        <property type="nucleotide sequence ID" value="XM_003326386.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KC79"/>
    <dbReference type="GeneID" id="10534383"/>
    <dbReference type="KEGG" id="pgr:PGTG_08264"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08264"/>
    <dbReference type="HOGENOM" id="CLU_1587302_0_0_1"/>
    <dbReference type="InParanoid" id="E3KC79"/>
    <dbReference type="OrthoDB" id="10367948at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000421">
        <property type="entry name" value="FA58C"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50022">
        <property type="entry name" value="FA58C_3"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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        </location>
    </feature>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="137"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP82015.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
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<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
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            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
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        <lineage>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178280">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08103"/>
    <dbReference type="HOGENOM" id="CLU_2043735_0_0_1"/>
    <dbReference type="InParanoid" id="E3KC96"/>
    <dbReference type="OrthoDB" id="10483274at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
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    <feature type="region of interest" description="Disordered" evidence="1">
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    <evidence type="ECO:0000256" key="1">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP81854.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="125" mass="13335" checksum="482D435698F4632B" modified="2012-04-18" version="2">MQKYKTQEEQPPSGLTTPASQLDWSRHSSTLDRLTGQVTPQLDGDNVLDEDDELPPPLDFTGTAMDPSPSLMTQRSFARATNGSGILPSWGDVSLTQASPTQAPNMSQPLSASMDDAGMDGVVDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCA5</accession>
    <name>E3KCA5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    </protein>
    <gene>
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    <organism evidence="2 3">
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        <name type="common">Black stem rust fungus</name>
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    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178280">
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    <proteinExistence type="predicted"/>
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    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCA9</accession>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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        <lineage>
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            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
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    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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        <source>
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    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
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                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="AlphaFoldDB" id="E3KD66"/>
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    <dbReference type="KEGG" id="pgr:PGTG_07496"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07496"/>
    <dbReference type="HOGENOM" id="CLU_970226_0_0_1"/>
    <dbReference type="InParanoid" id="E3KD66"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="287" mass="31260" checksum="D1B0A59F4E84D362" modified="2011-01-11" version="1">MHPTIETIDNVEHSTQTGLPSTEPMISSSKEDRRDDQEDSDRSMEIGCSSADSLSLFRSFSMPIHISSTTGSDQEEPLDRAQEDVNQDEEDEDFDELLTTPTSQLQLHDPSSYPSYQSSGQRLLSCSLPPSLSSQKSNGLDIHQEYPLNELLQLTQPMREGVISRERLLPSSRMKQVGIGGRGRRDFSNQRNPGCSSPFANHLSSAGAEWSATSPTKPDQASWSPASSCSPLPSSTFNHLSSSVGGSSANSQPILVTPQFASPTPLVRPRPIAAWQKPHGSWSFGSS</sequence>
</entry>
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    <accession>E3KDU4</accession>
    <name>E3KDU4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    <gene>
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    <organism evidence="2 3">
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            <taxon>Puccinia</taxon>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178282">
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08284"/>
    <dbReference type="HOGENOM" id="CLU_009635_1_0_1"/>
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    <evidence type="ECO:0000256" key="1">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
            <dbReference type="EMBL" id="EFP82328.2"/>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <sequence length="260" mass="29036" checksum="DDDEF3F0B606172E" modified="2012-04-18" version="2">MAKEIAAIIRQKNGDFNHYIDNNPQCFCHVLALILGAGLRSLKLQNQIQPPKSKPDFFPKLETIEQVVEPDGVYVDLTEDVDVQPQEINDLDDRDEVDPDDASEGKLSEDDQEKTPTDPTDKASKNGSTAGGIGHTLVKVDYISCQICSSTARRAKFKLVSQNIGYKGPQLIPGYGIRWNVVYDSWTQAYAAQKVIVQLLNDESDKYARKSAAGHFFKGYEVSNKEWQDLNSLNQVLEEFLAKTLRMEGNGTSSAMVLYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KEB8</accession>
    <name>E3KEB8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08847</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82651.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327070.2">
        <property type="nucleotide sequence ID" value="XM_003327022.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KEB8"/>
    <dbReference type="GeneID" id="10530034"/>
    <dbReference type="KEGG" id="pgr:PGTG_08847"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08847"/>
    <dbReference type="HOGENOM" id="CLU_018638_0_0_1"/>
    <dbReference type="InParanoid" id="E3KEB8"/>
    <dbReference type="OrthoDB" id="2496362at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172568" evidence="1">
        <location>
            <begin position="26"/>
            <end position="811"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82651.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="811" mass="93330" checksum="1B6A36D998AC2C9D" modified="2012-04-18" version="2">MRVFQSVLTNVRFVLFLSSYHPLYASIITPGSLHDAEAVGSFTPDARAGTVGTQGLQKEPMATTGANNLAHLNVPHSGRRSSYLNTPPLKELTEKMRKAATRTKMTLVDSYPDLRDQFYDASPVTIYKDLKTGREMRTQARSTDKMEEYRKVRDEVSEAYEKATKHENTLNLDKIANDYERRLSLGPYYRDWPAEVSGKSWLEDYLLQLKLRQSAINELEKVQQAWESLDFSRGLSQAEEEVKAEVSDFLKRAAQPGFVYKFRPEYDQMTLRVNSQLRKWSPNASIDPKIFFPNVPTLDHFEEYITSSSFILARAVQLKKRPAPERITHDDLVTVEQVIQTLPPEFDLEEELHHYAMIKAFEKILEVVRREATQLLKERFKRRPDKLLDSTQTEPQLRIIFDSRTEQDIAWVDKIFQYIYHTTPTELIEQLRGAPDDQVEMRATLDRNAEQLASLSEGDKKALRDASSDRQTFLKTLDEIAAHAPQELYSTVRRIKKEIASLKIPVEYELVDALHQRGIIDHATRSKLTSASSRQNFLQNEFRTTVMSEFKGFLVEELQRNKLNRLGSPEAKILDATAGLLAQTVVNQLNKDAQKFYMEAFLSSEYGFDKAMSFYDPNHLKNFDSLVIDEQTRFKAYVEACFGPDPSDYRPTRVQYTSIIDRFLSEIKIPQFGSKSHTLAKMNIRSPGLPKGSPDEILVKRLPNFDERLSTIFLKPLAMGDWRLVDTLFERLPEKAVTNLNAAAKTIYTNRRRIPELEEKIKILTEGIGRYEESTLSRKLPAVKTIMESVLEALKRHEKNFMHSDTGTRPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>E3KEH8</accession>
    <name>E3KEH8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Cytochrome P450-dit2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_08907</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82711.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327130.2">
        <property type="nucleotide sequence ID" value="XM_003327082.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KEH8"/>
    <dbReference type="STRING" id="418459.E3KEH8"/>
    <dbReference type="GeneID" id="10530094"/>
    <dbReference type="KEGG" id="pgr:PGTG_08907"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08907"/>
    <dbReference type="eggNOG" id="KOG0157">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001570_27_0_1"/>
    <dbReference type="InParanoid" id="E3KEH8"/>
    <dbReference type="OrthoDB" id="1470350at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017972">
        <property type="entry name" value="Cyt_P450_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047146">
        <property type="entry name" value="Cyt_P450_E_CYP52_fungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24287">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24287:SF1">
        <property type="entry name" value="P450, PUTATIVE (EUROFUNG)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00385">
        <property type="entry name" value="P450"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00086">
        <property type="entry name" value="CYTOCHROME_P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8 9" id="KW-0349">Heme</keyword>
    <keyword evidence="6 8 9" id="KW-0408">Iron</keyword>
    <keyword evidence="4 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 9" id="KW-0503">Monooxygenase</keyword>
    <keyword evidence="5 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="10">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173290" description="Cytochrome P450-dit2" evidence="10">
        <location>
            <begin position="17"/>
            <end position="559"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="8">
        <location>
            <position position="505"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP82711.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="559" mass="62832" checksum="E01933F1273D7C46" modified="2012-04-18" version="2">MSRVALLLSFVGPLLLSKFLLESFERNPSFLDRLVVFCLSWPIAREIRLRLEQRDLRARASSRGALLAPLVSSPLPFGLSVLLTRLRMIHSGSPGDVIHLFNSRVPKPIHPDQPTKVFRSRVMGVETIWTIDHDDAKYFLSTGFPNFGKSPLFKAGFRRLLGDGVFASDQRGLWAWHRSLTRPHFVRERIADVVAMEEHSHRVATWLSTQTDLGKSVDIQDIFARYTLTVGTQHLFGRCVDSLNDLIHDRIQTGPNAADFAQNFVAAQHWAIINSLLLHPLLISLGFRIRDRATEEVRQVVDTLVQDASLSLASQIKKNNESDSSDQAEGGTASENLLDHLLTSGCSKELVRQECLNILLAARDTTASLLSSCIYELARDSPRKTAMWRKLKDEVERLGSGIDVTLDQVRELKYLRAVLNESLRLHPPVWANTRHAFEDDVLPSGVFVPAGTDCRFFIREFQRNPEVWGKDAEEFDPDRWIDSRKALQVKDPFSFQPFSAGPRICLGQQFALAEASMMMIRVIEGFEGVDLDLSDGPVGAEAPAVVLSFRGGLKVRFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3KEJ2</accession>
    <name>E3KEJ2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_08755</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82559.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326978.2">
        <property type="nucleotide sequence ID" value="XM_003326930.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KEJ2"/>
    <dbReference type="STRING" id="418459.E3KEJ2"/>
    <dbReference type="GeneID" id="10529946"/>
    <dbReference type="KEGG" id="pgr:PGTG_08755"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08755"/>
    <dbReference type="eggNOG" id="ENOG502S6Z0">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_263328_0_0_1"/>
    <dbReference type="InParanoid" id="E3KEJ2"/>
    <dbReference type="OrthoDB" id="2013972at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041698">
        <property type="entry name" value="Methyltransf_25"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF24">
        <property type="entry name" value="2-METHOXY-6-POLYPRENYL-1,4-BENZOQUINOL METHYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13649">
        <property type="entry name" value="Methyltransf_25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase" evidence="3">
        <location>
            <begin position="381"/>
            <end position="479"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="49"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="141"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="887"/>
            <end position="906"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="925"/>
            <end position="946"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1128"/>
            <end position="1183"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1064"/>
            <end position="1091"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="228"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="929"/>
            <end position="946"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP82559.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1278" mass="142665" checksum="819A0FA045A59E53" modified="2012-04-18" version="2">MAYLQEAYLLVEPGDHEVSKHDRAHHRMYNSNHLASSSQHETSRIRRLLKKSAPSRRPTVESDPDINPELPVKSPLSRGHGASAVGHETGLDIKPLSKSLGQPLSRLTRSASRRSLVGVGGRFIPDNTLADNGPSIKRAVTEAKQGSPPATEELSYNHLDPSTSAPSEFPFNTAPPSQSTLSPPVLGNTKRKPRLSISKNMIQYCLSTPKHSPLAWPPIQNGASQISLHSAESEANSDTGPLASPKESHHGSKLIFNNFRKLTLNSKRSPVASSKSCLPADLTPHQPSFSDALPLSRTDAVRRQDHSNEALDHTWFSKSRGERYHPHLRNDVPYWMSYGPEIMDYHILMQYSSAILQGTSPFQLMASPPEADNSKREIRKVLDIGCGPSATWCVGVLRETSEVEVVGLDICPLLLNLDCLERRVSENLSFVKHDFSDCTLPFETASFDYVHASFVAAGIPEHKWPSMLEEMTRVLKRQGTLELLECNTLIADPIKAKDPTYLDDGKTQGTSFCRVPTASASNQLSVMPRETELLKESVSEVYPTTVKEMVDKLLESHFISPYPLSLLPTEVSNLTTAMRRPLAKKFIRFPSDLQSFVDTQTKSPDRAHTFIGNDSQQLVAKDDPIAKAAANECMGMLLLHSHINAMYSNKEISWCDLWLPAIHCETTGMHVPHPLVKRTRPTPSRMPPHFGLHLNQNRRTKSNSPIYKVKSEHSDQSVFETTSDKAEASNSKSIFTLHEGQTNSHENVKSMSHSNLNTDALAGNFNLDLPLDPRDSVQHQHEPEKMDIDSLRVSHSIISPHRKKFDQTWNKWKDDLNCHSLGISKLLELRFGWTCTMDLENHKALYEHYEFYQQQLAQCESRIAELRLKLQEIKFVHIDELEASLDEDLHSNPPSSVRGASPVYTHRPHSFEYETSSEGHEDFEHINFPRNSIGGSSHDTATSTDPRQLMSCSENPILGYGSNVVHHEPDSQLLEELGFDILKENSNMRPRTRHSSGSTGRIPAGLKDARSLYRKSSVAAFNRHQAESDNEEGFMSDSPISSKVFLSSSKVTSNHIHSKGRETDVDLETEIESLTRQKAEIKKAIQLIQTDLDNVTKRLGLLDPEDYQPLATSTAAVAQVSKLPLAGETDQHQVATPENCDKRSAPAAREISSAPSDEAKESSAKITTNPKGSTRDDKRTESGMMNDLFSPFDKLEFASFLVDDCYKPASLLGQSTQHEANKETNEDLTSRISIFNQNNPGRTSNLSHRHVKNAKDLRFTNVGFGDLRIEKFYSVKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KEN0</accession>
    <name>E3KEN0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08940</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327163.1">
        <property type="nucleotide sequence ID" value="XM_003327115.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KEN0"/>
    <dbReference type="GeneID" id="10530127"/>
    <dbReference type="KEGG" id="pgr:PGTG_08940"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08940"/>
    <dbReference type="HOGENOM" id="CLU_061016_0_0_1"/>
    <dbReference type="InParanoid" id="E3KEN0"/>
    <dbReference type="OrthoDB" id="10338309at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="266"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="62"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="288" mass="30857" checksum="57610A2B382D8BE3" modified="2011-01-11" version="1">MSSSAAIGRQARSKGLVSPLLPGSQKLPTPPINSDFRGIGQPLVRPPESGSMTFKPAEQAPFRSTQSSNIPTTVHSPETAEANRPQAYPLQRPPGNHHKMGSPYNHNPVQNTGRQVSEKSVTTSPPQLHPPQPLAPERIAGQEIPSRNNPILGSKAPRTSVQQVPMTHKLPPGVPNHVTKNSQPKENSFLVTSNLPAGSKSEVTNLSKLPTTQQIGIQQAKPPPPLSVKTSSRGPENPISQPNKKVTHQLPVPPFEPIMAVRNPRELAWMSRPGSQPLPAPPADGPIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>E3KF19</accession>
    <name>E3KF19_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">N-terminal Ras-GEF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_10019</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0005886">
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
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    <dbReference type="GO" id="GO:0007265">
        <property type="term" value="P:Ras protein signal transduction"/>
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        <property type="entry name" value="REM"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Son of sevenless (SoS) protein Chain: S domain 1"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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            <begin position="744"/>
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            <end position="2381"/>
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            <dbReference type="ARBA" id="ARBA00022658"/>
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        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00168"/>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
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            <dbReference type="SAM" id="MobiDB-lite"/>
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            <dbReference type="PROSITE" id="PS50009"/>
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    </evidence>
    <evidence type="ECO:0008006" key="8">
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            <dbReference type="Google" id="ProtNLM"/>
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    <sequence length="2385" mass="263846" checksum="0C0B44454DE698E2" modified="2012-04-18" version="2">MDRSKSRSISPFGSNSPSKSKPFHTQTILSKSAGVPSSGAFAHLPERPTGDSATDFTPAQKLPHSSQDSGSNLSDYPQPIASSTASSPSKKFPIKSSPKNNNNSNINSLKPHHHSPAPKSRPTSSSSRFSRQNAREADVLGKILGWRQSKTLRASNSFKSSPLHQTNKHPHSSSKHLIRSTPPQELPEIFRPSNTHALSSALNTSQDSTSEKPSSGDVTQPHDFLTDTNYQTASRPYFHQQSSSDLTSSTHASSNLSSSVTHANSSQTSFNQPSPSPTQPVQLQPNPDGLPRHSQHQSSGESKLTVLRRPILKKSFSSGQLNHHFNSSHDPSKPTSQPSSFSRLSQSTELSSRDPHPCLSGLSINPPEALDPPISFASPHGIEGLSSASLSQLNKPRYISQYSHQRLSLPAMHAILDANSSLAQTIQLDQACSDDSDHSVQPIGTLLKHPTMHGVASNESIRTAKPSTEFNSLKSLSGFLPLADHQPSPGADHHDNNIPSSDHHGLRSSDLQDSTSSARPRRFQNPRVFDIFTHTHRRDPVDLMRRLLSPELTGSSTPSETSSNPSHSQSLGVSINSSLNSSNLLGSFANFNSPFNKDPRFVIWAESPSSAPRQQSPANVYPASRKRSSATVETRHYPHHPASASTSNLGIDHSTGRLTHALNPTSVAFTSPSSPSIPKRWSRSARTPTSHHSNIDSLSNSSPNLLTDVRHPHDDSGHRRSREELSQLHSTISHGEEHTPKLGNDRILMAATIERWVAELTTHIDSLGLVEFFYTYRAIIKPTELAQLLASRFEWSIMFRSLSYAKTLPDWNLHSVLVDPNLEKGILGLDGRLISVDLQGETLRRVAKVRTFVMIRHWLLHHFEDDFLTDVSLQTELHNWVENLIEKIKRVQRIISRLDNRTPSDAEESNHHHPTTPSPFSGEDDLRILKSLRKVLKELQLTHHQSFNVAVRPGSTPIDQLDDDDKQGRLNRKSTKVDVRDLFAESDKRNDQTSARRNRRSHTADLTDLQRSSPKVDIGHGRAHSINTLVQVRASNFARSDLRLCEADHNATNSPEVLQEEENPRASTEDVDLSKSDEKLILKLKQVFQFDTRRAREGVYHQIISKEIDSDPSRHSLHQANITLPKQHPHQMMISRMRPPESTSDLPKSHNAAQPRLNLSNHTPAPFNQGSLTFPQHHNPFQRYFTSTMGTFGRFKRMINNRSGPHLASNAAITSNHYTGVASGVDSFSSSLTRETGSQQDPQQNEVGDLLCAKGGLERYLNFFEIQKDSSISSYQENLTKEPIQVDCNGLSKPKEPANTDSGPESMVEVDQSSGSPVVQYDDCSESQLSLSSSLSNTTCSTQSMHSESGGEPKTEASTADLVVVSPDVSGEQHMITKSTPARDEHLLHGSMSTETIKSEAHHLALAGGGMDLLKKQLRKKFTNKDMCPVLMRASMDESFSGTVPEHSNSFTRHSEDSPRLNAFSLSVSAPNGEKDLSERPTSVQLDDIDLLDSDEDSPVGVQKLRRLPGAVDLKQAIAFGRKSLFNLKGGLLKGNDDRSKRFSTETSSSIGTRRVPSIAASMFRRSKLSHRSSIPNSTTPSLAPSAQESSLSEQNEADSGTPGVVANFVADGLESDDDEPGDVEAALRRLEGVIDADREKEKARKVELQMIKSMEASRRTRTFSHPTSDDRCSYNSQTDEADELETCSESAEDDEASEESDEDVLDAHNNEAEGEDEDERDEQQDREEQVEHEDGEEDDVNEEKAVQLQESTCSSEPTRKSMSILLERSALNSPIAEGDEEEADTQPIPAPNLSPTMPGSIEYSNPSTANFAEASLASSKPPVLTTQNHAPASKPGIRFFTAHSKGTSLDDIGLATNHSEDHVKTLRGNGKRLATRSQQVMTRKSSLHKLFSANNNPPKSSSQLKPKGIASGSIPPLPPPPYNSTTGKPAIIHSLPLPPVHRSFLLDCRTEILAQQFCLIERDLLRNITWQELLLSKWQEDRSSSHHHGYLNGGTGQRGTRKKKGVPAEDSVTCWETFMKQRAKEKMRIKGRIQSNQNFATSSSNRLMDVPAQKLDGTEINALIMRFNLTCNWVASELVLTLNLDERVALLGKFIRLAFKCYRQNNFQTLTQIIHGLQTPYVERLKKTWSKLGIWESRMFRDLKEFTSHLSNFKSLRNVQDSLINDSPSTLSSSSATQTTTSTSTQHSINSPNTSAGNLPGNVSAHFLHSSEAPASSNIRTSHNYNRSSNSNNACIPFLGLYLRDLTVNDELPTYLDPTDPSIPVEIDPKTGKLKTLSNPKVFEDLPRLPTIGEPVKSSSDEPDRPLPKQEDDDIDSTEHGYYPLVNINKLRTYAKIILKIKEFQTRSIRNYTFETDSKWFLTCLKIKCLSSEQLRLLSKTCEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGW3</accession>
    <name>E3KGW3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerases I and III subunit RPAC2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_08724</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the archaeal Rpo11/eukaryotic RPB11/RPC19 RNA polymerase subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327957.1">
        <property type="nucleotide sequence ID" value="XM_003327909.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KGW3"/>
    <dbReference type="FunCoup" id="E3KGW3">
        <property type="interactions" value="224"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KGW3"/>
    <dbReference type="GeneID" id="10532220"/>
    <dbReference type="KEGG" id="pgr:PGTG_08724"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08724"/>
    <dbReference type="eggNOG" id="KOG3438">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1705100_0_0_1"/>
    <dbReference type="InParanoid" id="E3KGW3"/>
    <dbReference type="OMA" id="FTDEGHT"/>
    <dbReference type="OrthoDB" id="510325at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055029">
        <property type="term" value="C:nuclear DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006383">
        <property type="term" value="P:transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07029">
        <property type="entry name" value="RNAP_I_III_AC19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.10:FF:000006">
        <property type="entry name" value="DNA-directed RNA polymerases I and III subunit RPAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00261">
        <property type="entry name" value="RNApol_arch_Rpo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009025">
        <property type="entry name" value="RBP11-like_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008193">
        <property type="entry name" value="RNA_pol_Rpb11_13-16kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033898">
        <property type="entry name" value="RNAP_AC19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022905">
        <property type="entry name" value="Rpo11-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I,II,III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946:SF28">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13656">
        <property type="entry name" value="RNA_pol_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01154">
        <property type="entry name" value="RNA_POL_L_13KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RBP11-like dimerisation" evidence="7">
        <location>
            <begin position="35"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP83538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="154" mass="17606" checksum="9562C30390B10166" modified="2011-01-11" version="1">MDLDPTHQSTHDPAAEAILADPEKVTILPGDKYDAMTFSLKDEDHTLGNSLRYIIMKNPEVEFCGYSLPHPSEPKLHLRIQMYDNKSAAGALAKGLDDLEQLVLVLAERYRAELDRGKFERFEEPTVDERLEDIKRRKYPHLLKDPQGDITMNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>E3KH37</accession>
    <name>E3KH37_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FAD-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_09325</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328031.1">
        <property type="nucleotide sequence ID" value="XM_003327983.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KH37"/>
    <dbReference type="FunCoup" id="E3KH37">
        <property type="interactions" value="157"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KH37"/>
    <dbReference type="GeneID" id="10532297"/>
    <dbReference type="KEGG" id="pgr:PGTG_09325"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09325"/>
    <dbReference type="eggNOG" id="KOG2614">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009665_1_1_1"/>
    <dbReference type="InParanoid" id="E3KH37"/>
    <dbReference type="OMA" id="AYCPSAM"/>
    <dbReference type="OrthoDB" id="655030at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071949">
        <property type="term" value="F:FAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002938">
        <property type="entry name" value="FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051704">
        <property type="entry name" value="FAD_aromatic-hydroxylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46865:SF2">
        <property type="entry name" value="MONOOXYGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46865">
        <property type="entry name" value="OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01494">
        <property type="entry name" value="FAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00420">
        <property type="entry name" value="RNGMNOXGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0274">FAD</keyword>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="12"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="374"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="FAD-binding" evidence="5">
        <location>
            <begin position="13"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP83612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="418" mass="45824" checksum="8D32F7DAA43F844E" modified="2011-01-11" version="1">MTDSNHAPPKSLRVLISGAGIGGLVAAYWLGKAGASVTVLERAGEIRREGHVIGIRKEALPIVENMGLGEALRGRKTGELGLRIVDGFHNAWASIPLSDAGFTCPLQIHRGDLVTALYEATKDKASFIYDDSIDTVEEINETLRVTLTNRKSQALDFDVLMVAEGMRSHTRAKIFNRPVQEPILSLGLLVATFSFESDQAWASCYHIPDHRAILVRPDRHGRAYAHAICRGRSRKPAIGPQPSKDQHRAQFFQLFQKTGWESEKITKALGEAGDLHIQEVAQVRCKVWSKGRTVLLGDSAYCPSPIGGMGATAAIIGGYNLAVELIKNPADPQAAFRAYELSLRPWIDAIQKPAPAIAKLGFPETRLGILSLHFLVYLYFASPARLLFLPLILLLKHFGFFKPTRPPPVPSPSVFAQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHR7</accession>
    <name>E3KHR7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_09555</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EFP83842.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328261.2">
        <property type="nucleotide sequence ID" value="XM_003328213.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHR7"/>
    <dbReference type="GeneID" id="10532524"/>
    <dbReference type="KEGG" id="pgr:PGTG_09555"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09555"/>
    <dbReference type="HOGENOM" id="CLU_132785_0_0_1"/>
    <dbReference type="InParanoid" id="E3KHR7"/>
    <dbReference type="OrthoDB" id="10293889at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172011" evidence="2">
        <location>
            <begin position="27"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP83842.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="147" mass="15740" checksum="C14A44E006D4667D" modified="2012-04-18" version="2">MALLPYTCKMILISLYIFAFSMRVDSQPISLQEIADQHATLMPRHPQGTFSSTSDIQGRVSSLSRHTASDLAGPERSIATAPNWPPSSSSSYQRSMAAAPGSNGRRSAPYPVGASMARVQPGQLARPGSGSTHSISVCSYDESYGCW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIE8</accession>
    <name>E3KIE8_PUCGT</name>
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        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_10451</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84073.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328492.2">
        <property type="nucleotide sequence ID" value="XM_003328444.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIE8"/>
    <dbReference type="STRING" id="418459.E3KIE8"/>
    <dbReference type="GeneID" id="10545174"/>
    <dbReference type="KEGG" id="pgr:PGTG_10451"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10451"/>
    <dbReference type="eggNOG" id="KOG0109">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_628729_0_0_1"/>
    <dbReference type="InParanoid" id="E3KIE8"/>
    <dbReference type="OrthoDB" id="1099063at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006397">
        <property type="term" value="P:mRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:000952">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.60.10">
        <property type="entry name" value="Zinc finger, CCHC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050502">
        <property type="entry name" value="Euk_RNA-bind_prot"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001878">
        <property type="entry name" value="Znf_CCHC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036875">
        <property type="entry name" value="Znf_CCHC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025">
        <property type="entry name" value="OS02G0815200 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48025:SF1">
        <property type="entry name" value="RRM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00098">
        <property type="entry name" value="zf-CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00343">
        <property type="entry name" value="ZnF_C2HC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57756">
        <property type="entry name" value="Retrovirus zinc finger-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50158">
        <property type="entry name" value="ZF_CCHC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0507">mRNA processing</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RRM" evidence="6">
        <location>
            <begin position="111"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="CCHC-type" evidence="7">
        <location>
            <begin position="197"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="219"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="419"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="46"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="232"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="266"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="289"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="419"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="437"/>
            <end position="446"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50158"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP84073.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="485" mass="56204" checksum="763996C59CDA80CE" modified="2012-04-18" version="2">MLAQKDMTRPQIDQHHQREDYRATRSRVEGDVRDSDRRGFHQSSLPRERHTESRPLPRDSPPRPPPPHDRNAERIYRDPPGPETVPHGASLPVDAHGPVDSIQNAPVPNPSKIYIGGLPELTRPEDLQDCFSQLGRIKNIELKSGYGFVEYDSPQAACDAVSKYHEGTFLGSQIKVELSHGGSRPRHVAVSNNPEACYTCGIIGHWARDCPDADSQLYPKRRLRARSPTPPRHRENAYMNRDSRPEELSSHEYRPHRRRSPLGEIDYSRTERERLPPSSRESYFQPAYLEDKRYRPEHSLRDRERDEPRALDLKILPRNDDHRAPYLITPSRSRSPPPGSRDYYTAERTRPAAPYPIRSLPRDDYQSRVHVIKPYAPEPTYYRDHVVRAPETTRVSRYEDRSPGRPHWAHPAERVVEPTRIDRRYEAADARIPARGHSPRRARSPRRSPSPSRRPLDHRRLAGRIHSPPPYEQTGYARPAYPIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIK1</accession>
    <name>E3KIK1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10504</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328545.1">
        <property type="nucleotide sequence ID" value="XM_003328497.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIK1"/>
    <dbReference type="GeneID" id="10545304"/>
    <dbReference type="KEGG" id="pgr:PGTG_10504"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10504"/>
    <dbReference type="HOGENOM" id="CLU_171248_0_0_1"/>
    <dbReference type="InParanoid" id="E3KIK1"/>
    <dbReference type="OrthoDB" id="2503667at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="114" mass="12217" checksum="C1BE0041D40A038F" modified="2011-01-11" version="1">MSISLMISKRALQSPVLRSNYPQRIFVSSFSTTPKSNDIVGKVQDAAQTVNKKASELASKGMENLEAASNKVKQATSSASSQARNVAADAKNEANKTKESAKDMAQDAKKKVQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIW3</accession>
    <name>E3KIW3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10616</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328657.1">
        <property type="nucleotide sequence ID" value="XM_003328609.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIW3"/>
    <dbReference type="GeneID" id="10545499"/>
    <dbReference type="KEGG" id="pgr:PGTG_10616"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10616"/>
    <dbReference type="HOGENOM" id="CLU_787873_0_0_1"/>
    <dbReference type="InParanoid" id="E3KIW3"/>
    <dbReference type="OMA" id="EPKPMPH"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01217">
        <property type="entry name" value="PRICHEXTENSN"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="352"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="63"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="352"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="352" mass="37757" checksum="31F145753527BCA1" modified="2011-01-11" version="1">MEDLIPKPPPNTNPTIPIPPSTQPVPIKPALKQPIPTKPPSGVPKPPDADFSVKLAGSQIQPKPLPTIPKQPQPIPTQPQPIPTQPQPIPKQPQPIPKQPQTIPKPPPKIPQPLPNIPPDNRFSELTSSPPPPPPKKTIPQPLPKIPPTERSNEIPQPPPKSSPQPSPKPEPKPEPKPEPQPAPKPLPKPDPKTPTKPEPKPEPKPEPEPKPAPKPEPKPAPKPEPKPAPKPEPEPKPAPKPEPKPAPKPEPEPKPAPKPEPKPAPKPEPEPKPAPKPEPKPAPKPEPEPKPAPKPAPKPEPEPKPAPKPEPKPAPKPEPEPKPAPKPEPKPAPKPEPEPKPDPKPDPGAKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJF6</accession>
    <name>E3KJF6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase</fullName>
            <shortName evidence="11">HMP-P synthase</shortName>
            <shortName evidence="11">Hydroxymethylpyrimidine phosphate synthase</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_10151</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Responsible for the formation of the pyrimidine heterocycle in the thiamine biosynthesis pathway. Catalyzes the formation of hydroxymethylpyrimidine phosphate (HMP-P) from histidine and pyridoxal phosphate (PLP). The protein uses PLP and the active site histidine to form HMP-P, generating an inactive enzyme. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(6)-(pyridoxal phosphate)-L-lysyl-[4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase] + L-histidyl-[4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase] + 2 Fe(3+) + 4 H2O = L-lysyl-[4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase] + (2S)-2-amino-5-hydroxy-4-oxopentanoyl-[4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase] + 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + 3-oxopropanoate + 2 Fe(2+) + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:65756"/>
            <dbReference type="Rhea" id="RHEA-COMP:16892"/>
            <dbReference type="Rhea" id="RHEA-COMP:16893"/>
            <dbReference type="Rhea" id="RHEA-COMP:16894"/>
            <dbReference type="Rhea" id="RHEA-COMP:16895"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:33190"/>
            <dbReference type="ChEBI" id="CHEBI:58354"/>
            <dbReference type="ChEBI" id="CHEBI:143915"/>
            <dbReference type="ChEBI" id="CHEBI:157692"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:65757"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 11">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the NMT1/THI5 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178290">
        <property type="protein sequence ID" value="EFP84431.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328850.2">
        <property type="nucleotide sequence ID" value="XM_003328802.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJF6"/>
    <dbReference type="FunCoup" id="E3KJF6">
        <property type="interactions" value="93"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KJF6"/>
    <dbReference type="GeneID" id="10545941"/>
    <dbReference type="KEGG" id="pgr:PGTG_10151"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10151"/>
    <dbReference type="HOGENOM" id="CLU_028871_6_3_1"/>
    <dbReference type="InParanoid" id="E3KJF6"/>
    <dbReference type="OrthoDB" id="434407at2759"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
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        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13650">
        <property type="entry name" value="PBP2_THI5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000187">
        <property type="entry name" value="4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase THI5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027939">
        <property type="entry name" value="NMT1/THI5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015168">
        <property type="entry name" value="SsuA/THI5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31528">
        <property type="entry name" value="4-AMINO-5-HYDROXYMETHYL-2-METHYLPYRIMIDINE PHOSPHATE SYNTHASE THI11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31528:SF1">
        <property type="entry name" value="4-AMINO-5-HYDROXYMETHYL-2-METHYLPYRIMIDINE PHOSPHATE SYNTHASE THI11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09084">
        <property type="entry name" value="NMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SsuA/THI5-like" evidence="12">
        <location>
            <begin position="16"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF09084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP84431.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="341" mass="37606" checksum="1B73C1D303A63D36" modified="2012-04-18" version="2">MSTDKISVLLNWHATPYHLPIFVAQSKGYFAKEGIKVAILEPNDTSDVTELIGSGKADLGCKAMIHTLAGKARGFPIKSIGTLMDEPFTGVIYLEGSGITSDFKSLKGKRIGYVGEFGKIQIDELTKYYGMTSEDYTAVRCGMNVSKGIIEGTIDAGIGLENIQQVELEEWCKANNRPASDVKMLRIDELAELGCCCFCSILYIANEDWLKAHPKEAGAFMRAVKAGADDMFADPRASWAEYCKVKPVMNTPLNRLMFDRSFNYMSQDLSNVSRDWNKVTNYSKRLEIVPADFVANYTNEFVQWEVAPEGGEAEGLAKQQEIKALQAHVAQHGGVLQATVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJY2</accession>
    <name>E3KJY2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_10766</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329026.1">
        <property type="nucleotide sequence ID" value="XM_003328978.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJY2"/>
    <dbReference type="GeneID" id="10546276"/>
    <dbReference type="KEGG" id="pgr:PGTG_10766"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10766"/>
    <dbReference type="HOGENOM" id="CLU_1797426_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJY2"/>
    <dbReference type="OrthoDB" id="2509786at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP84607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="144" mass="15712" checksum="804590D7635C8EF7" modified="2011-01-11" version="1">MLTNSLSNSTISFCFSPDTSISSFTSCGSHLAVLGELVQCEEEYKAALRVPISLQNALAPLLDMKTTAHPIIQRDWYQEAEIGLILYLAGTSSASSEEQSASLCQYSLALEASLYAEENRKLAGQTKLSPTSIIKSIKNWWISS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3KK53</accession>
    <name>E3KK53_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_10837</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84678.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329097.2">
        <property type="nucleotide sequence ID" value="XM_003329049.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KK53"/>
    <dbReference type="STRING" id="418459.E3KK53"/>
    <dbReference type="GeneID" id="10526645"/>
    <dbReference type="KEGG" id="pgr:PGTG_10837"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10837"/>
    <dbReference type="eggNOG" id="KOG1395">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG4175">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_328485_0_0_1"/>
    <dbReference type="InParanoid" id="E3KK53"/>
    <dbReference type="OrthoDB" id="343092at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048476">
        <property type="term" value="C:Holliday junction resolvase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031573">
        <property type="term" value="P:mitotic intra-S DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000712">
        <property type="term" value="P:resolution of meiotic recombination intermediates"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.670:FF:000004">
        <property type="entry name" value="Crossover junction endonuclease EME1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.670">
        <property type="entry name" value="Crossover junction endonuclease EME1, DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042530">
        <property type="entry name" value="EME1/EME2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033310">
        <property type="entry name" value="Mms4/EME1/EME2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21077:SF5">
        <property type="entry name" value="CROSSOVER JUNCTION ENDONUCLEASE MMS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21077">
        <property type="entry name" value="EME1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="12" id="KW-0469">Meiosis</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="77"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="690"/>
            <end position="716"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="119"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="146"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="205"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="239"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="265"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="353"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="389"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="483"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="504"/>
            <end position="528"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="703"/>
            <end position="713"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EFP84678.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="875" mass="96643" checksum="3688C1B62798C84D" modified="2012-04-18" version="2">MIALVAALNGLGKWRRDPCDYNRATRRASAPLLFTRRVGLLPLEWHQSSLNTTVISQSTDLLAKSRMDSDSDIEIIPSKAPASQGTTQTKQTQPKPKPTVVRDRPPSLSPLPLPKVLKRPAEKSIDVSPHAEPEKDCERSNKRSHQSSSASIAHSLRPSSPELPEPSRHSFSSELPAPLQLSPINSPQAQEDRQDQTRTFDPVSSPISRKSQSQGVIELGSSPLVDIPSSFTKARMPISNNLSRQPSSSSSSGPSLKSLAKEQTCRANSNPKNPHQSGSARQQSKELSGSPIPFRNSHKSGQEAKELSASPIPVRKKSQNIVPPVDPDSSGELSDLDSILARHNPSLHAPNRKRNDSTADKHQNLNDDLGVSTRDQFPALTGPLHRNHTSSAVLQSAAQSNKGLPRSHSAPQKSDKPQNAVDKGKGKAAQTSVTPLSLIDPGDENLLSDLDDFNFNYNRNPSPPAHLPSRPSSTHPSPPPNSAPVSKAAASRIEKQAQAAQARLLREAKKAAKEKEKQDNRLQREANKLTLNRKETVAEVRMWVPKSLKKSKLHPFAKACEVLEERISSTEGCVMRPPGEIDDEDSEPKGVVRWVRTATKEWDEERGIFVPFGLGKTVETSEPTVLVVWTGKDVDEVIASGSDQLMKKAQAIKAHYPQDQLFIVISGLETILKAERRNEDSRIMDEARERLAAQRNEPPPCPSKSKSSGSTMPRNSRDKILKELEVMKIVTKAFVIRVEEKNEFANWLWEMTMEIGVRPYKSRKQELKSALRLEVSGTKGTNYEDTYIKMLSSLTRVTENEAKGIVARFPTLKSLYQSWERGIEKNGLVWAEDMLVGCSKSNSITNVNSNRKIGKVTSKRIFEIMYKAKNGDACL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KKX3</accession>
    <name>E3KKX3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10419</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178292">
        <property type="protein sequence ID" value="EFP84948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329367.1">
        <property type="nucleotide sequence ID" value="XM_003329319.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KKX3"/>
    <dbReference type="GeneID" id="10526923"/>
    <dbReference type="KEGG" id="pgr:PGTG_10419"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10419"/>
    <dbReference type="HOGENOM" id="CLU_1245901_0_0_1"/>
    <dbReference type="InParanoid" id="E3KKX3"/>
    <dbReference type="OrthoDB" id="2507816at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="147"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="222" mass="24712" checksum="9D0E33B325DB352F" modified="2011-01-11" version="1">MRLAKSSLGLRDGYGDRERVKRREILARLETEGIIAEEQGYLTTSSRSEKTKSPIEPRTPSPSMGPSAVRILTPAVMPGSVEAMIPRRRKLSHKLSIKSFRQWIQNQRRPLVNNSSSSSSGTQPALLTTHLKQPTPLRSQSDRVFKPARSSDSLSPTSSDRSAELIQPDRTPLPTISPPPPPTARKLSSKESFIWYSPPAQALTRLHRLESGSYDQEETAVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLC6</accession>
    <name>E3KLC6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Elongin-C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_11270</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SKP1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP85101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329520.1">
        <property type="nucleotide sequence ID" value="XM_003329472.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLC6"/>
    <dbReference type="FunCoup" id="E3KLC6">
        <property type="interactions" value="322"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KLC6"/>
    <dbReference type="GeneID" id="10527082"/>
    <dbReference type="KEGG" id="pgr:PGTG_11270"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11270"/>
    <dbReference type="eggNOG" id="KOG3473">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_130038_2_1_1"/>
    <dbReference type="InParanoid" id="E3KLC6"/>
    <dbReference type="OMA" id="AMVSPII"/>
    <dbReference type="OrthoDB" id="249087at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070449">
        <property type="term" value="C:elongin complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000035">
        <property type="entry name" value="Elongin C transcription elongation factor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039948">
        <property type="entry name" value="ELC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001232">
        <property type="entry name" value="SKP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20648">
        <property type="entry name" value="ELONGIN-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00512">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP85101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="112" mass="12367" checksum="6329F2E10F441EE9" modified="2011-01-11" version="1">MVQNSNGSKYVTIVSEEGFRFIIPKQAAVLSNTLKDMLSEDIGMLESESGVVNLMHSAPVVEKLCHYLLFRHHHITTPDGQSAGKGSAHNFDDQIPIELALQLLECADFLDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNT1</accession>
    <name>E3KNT1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_11712</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178297">
        <property type="protein sequence ID" value="EFP85956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330375.1">
        <property type="nucleotide sequence ID" value="XM_003330327.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNT1"/>
    <dbReference type="GeneID" id="10539955"/>
    <dbReference type="KEGG" id="pgr:PGTG_11712"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11712"/>
    <dbReference type="HOGENOM" id="CLU_543065_0_0_1"/>
    <dbReference type="InParanoid" id="E3KNT1"/>
    <dbReference type="OrthoDB" id="10292586at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP85956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="502" mass="58594" checksum="5F96090F7A4487F1" modified="2011-01-11" version="1">MYIKQIKNVHAEITGSILKETHQEENFKNRINVNTSKRAEAQKESLQEDPQTTNCFKSMIKAAEKQELDMENDFKKRIQAAFKPRGESEDEVYPTVVKPKWGNAVHDGEISGLLKESIQSVKKMAEKEKLSQSEAAESWTFQKALEILEPYLQPTNPQAHHPVPRITDEAYQSLKFLWTFHPASFLRIRASLSSRLDNKWEVQRRLVTVTTQITQKTIVENWMLIRNALLDGLQNERRTSLKFYEVRLQLAREFPNIVYYSDLFLPIFSRILEDEVANTGQTLLPFLRSWMGDHESSRRLKTPISERLYQLKIELSQDLSENLRASEIARGVDVWTVHHWIKCLGLGQPKKLDDWTDDEVLFSMEKLQKLIAGMSRNEGIPWHESASRAWLIETFGQEIYTQQLDLLCNKYKQIRARLVSHEDHRWKNNGSSVIKILDGQEEDKLKFADHGLDFKLIQAIKIIRSVNQQFRADWNSLFDSCPSKLTSNQKDFIKIWFADNFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPM2</accession>
    <name>E3KPM2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bicarbonate transporter-like transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_12213</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178299">
        <property type="protein sequence ID" value="EFP86257.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330676.2">
        <property type="nucleotide sequence ID" value="XM_003330628.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KPM2"/>
    <dbReference type="FunCoup" id="E3KPM2">
        <property type="interactions" value="124"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KPM2"/>
    <dbReference type="GeneID" id="10540268"/>
    <dbReference type="KEGG" id="pgr:PGTG_12213"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12213"/>
    <dbReference type="eggNOG" id="KOG1172">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002289_7_2_1"/>
    <dbReference type="InParanoid" id="E3KPM2"/>
    <dbReference type="OrthoDB" id="1735926at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005452">
        <property type="term" value="F:solute:inorganic anion antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046713">
        <property type="term" value="P:borate transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006820">
        <property type="term" value="P:monoatomic anion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050801">
        <property type="term" value="P:monoatomic ion homeostasis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011531">
        <property type="entry name" value="HCO3_transpt-like_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003020">
        <property type="entry name" value="HCO3_transpt_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453">
        <property type="entry name" value="ANION EXCHANGE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11453:SF38">
        <property type="entry name" value="ANION TRANSPORTER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00955">
        <property type="entry name" value="HCO3_cotransp"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="69"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="109"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="215"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="252"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="302"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="342"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="522"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="7">
        <location>
            <begin position="38"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="7">
        <location>
            <begin position="217"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="Bicarbonate transporter-like transmembrane" evidence="7">
        <location>
            <begin position="433"/>
            <end position="570"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP86257.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="627" mass="70233" checksum="7142F6BEED1AAAEB" modified="2012-04-18" version="2">MNPTSPVTSGEYDFPSDQQPLSDGKPWSQRSWSYQFMPCRGIYFDLKRRIPLYSSDWCFNFKANKLIQVFSATLRMYFINLMPAIAYLIDMNDRTGGIYGINEAVLASALAALVFSLFSAQPLTIVGVTGLINLFNYTDYDIVKDHGINYLQFQAWMLIWAAIFHFLMAIFNLCDFTRFITDMTSETFGFYVGVIYIQKGIELLTREFSHSATDGWLSVVVAISFALTVYWVEKIRNRGFGPLWARRILADYAFVIATVFFTGFVHIPGYLKSADLVKLPITQSWKPTINRDWVVDFWNLEARWVFIALPFGFLLTLLFYFDHNVSSLMAQARHFPVEKPAGFHWDFFLLGVTTFISGILGLPAPNGLVPQAPVHTESLSVLQHVSSDVPDRDGVVHPDLVKHDQERRRRIKDSGETGGSVDNQLPACKIVRTEVAEQRLSHLGIGLLTLGTMTRPLLVALGTMPRALFAGIFIGVGWSSIEDNGIIGKTLYLIRDPEMTPPNHPLNALRKITILKFIGIQWFTFAIMVAISQTIAAIGFPLVIIALIPFRYYYGPRWFTPAELSLLDSPTANALGVMVSIGGDLSRVTGEGLEVAPDTGFLGSLGLNDKLNPASQSDADLDRRKTE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQ71</accession>
    <name>E3KQ71_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12402</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP86446.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003330817.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQ71"/>
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    <dbReference type="KEGG" id="pgr:PGTG_12402"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12402"/>
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    <dbReference type="InParanoid" id="E3KQ71"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="76"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86446.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="367" mass="41171" checksum="287463C84C0ECAC0" modified="2012-04-18" version="2">MAEKTDVHPLKGIDYSEKDIGVLVRQLCKNLNQQHSQCLTEVVDFDLQAVFLMQEIGPHHRIRSIVKMVLKQFDINTQPPKTPKKRSPSSAPPQNMLQQQLLDAAKQVQKTIDTFDNNTSSRKPFCLQFPSYKHVLQNAISMQDKCQKLPTNRMVVLLDEAGICVGVVVPPFPKTPGKHHPEADDCACQALNKTVLTSSWNTNEDEVQYSSTPLDPHPRSPFPLGKSNKGESRTPKGGGDTKTSMSFQTYGFGLGDTKSKGMNDLKIPILKNQINTAKLQGYSNSWKNVPFTPSLQITTQSQIYKTQARVHLQNEIKFYSKLSLWINKSFLPASTKITEQGVQYLMSSDSDLLRKNLAKENNQIIAC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQ91</accession>
    <name>E3KQ91_PUCGT</name>
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        <submittedName>
            <fullName evidence="6">Atypical/BCR protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_12422</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
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            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
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    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP86466.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12422"/>
    <dbReference type="eggNOG" id="KOG4269">
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    <dbReference type="HOGENOM" id="CLU_001762_1_0_1"/>
    <dbReference type="InParanoid" id="E3KQ91"/>
    <dbReference type="OrthoDB" id="185175at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005938">
        <property type="term" value="C:cell cortex"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
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        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <dbReference type="FunFam" id="1.10.555.10:FF:000049">
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        <property type="entry name" value="Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)"/>
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        <property type="match status" value="1"/>
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    <dbReference type="InterPro" id="IPR050729">
        <property type="entry name" value="Rho-GAP"/>
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    <dbReference type="InterPro" id="IPR008936">
        <property type="entry name" value="Rho_GTPase_activation_prot"/>
    </dbReference>
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        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
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        </location>
    </feature>
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    </feature>
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        <location>
            <begin position="618"/>
            <end position="630"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="2">
        <location>
            <begin position="696"/>
            <end position="722"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP86466.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="972" mass="105971" checksum="BE81B849FB280CE2" modified="2012-04-18" version="2">MSTLSMPPNRSASTLSTDSGSIPKLTTTNPANKIEAIESLSLSGNESNELPVLTENPEPQESDSFTIPAYSSSKSEDPSSSSNLIPKTSSSSSRSNLSPTHPSQPPLPPLHNSSSSSTSTSSQQQQPQQYHQQQQPPSTPAANSFPHSAQPTAKRRMLTPTFLPFTQIKVTNSHIRANEKSKEVISFSININIIIPPESDPERKGAKASWVVEKFYSDVLTLDHLVKSKHSKSQNKALAQLPDRALFKDHAPSKVDARKAVLQKYLQSLCTAQLKEKGDVCDFFNSNIVISERASVPNRPGFMQGYLTKKGRNFGGWQTRFYVLDGPILEYFDTRGGNRLGHIVITGAQIGRQQPRPQEINDDSYRHAFLIRMHKREKEGEVDHILCAETDEERDAWVDALTCYVTGRYVSADPGAVASIPPATSGSSPDSTPSRPAPNSNSYSSFSDNRPGRQTSLDGTSRADGPHPASTSSHHQHSSSSDLRTESKSSRNAHLRTDSINSSSFNPSASSSHPTENPTPTILEEPLSSNSTTTRKISSEQPQPPNSEPVRHKSAVSDNRSATLVDARSPPSTSNNNNEAQKPSKPPSQSQQSQSQQQQSSNAVPLTPRTNIQNRHLQQTIQQGSPQQESGIVGSDGDSITTLERPMTPESQQRPKISGPMNGAPITGGYRIKPAEEKKAKFRSAFWGFAGRTTERGGGVGGGGGAGGGGGGQSGGTSGGLGSPALFAPTPRPVFGVSLQEAVSVARVHEGLDLPAIVFRCVEFLEAKGAIEEEGIYRLSGSSVIIKGYKERFNTEGDFNLLESSKDEYHDVHAVAGLLKQFLRELASPILTRELHGEFLKVIDLSQRSDKVNELGRLCSELPLSNYTLLRFLSAHLIHVVQNEKINKMSLRNVGIVFSPTLGMPAPLFALLLKEFDLIFHIDGDNRARFKLPDDLKHLVLISTTRPYLQELELHRLILHQRRAALIRLARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQI8</accession>
    <name>E3KQI8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="19">AFG3 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PGTG_12945</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="16">
            <dbReference type="Rhea" id="RHEA:13066"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Mitochondrion membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the peptidase M41 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178301">
        <property type="protein sequence ID" value="EFP86563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330982.1">
        <property type="nucleotide sequence ID" value="XM_003330934.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQI8"/>
    <dbReference type="FunCoup" id="E3KQI8">
        <property type="interactions" value="242"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KQI8"/>
    <dbReference type="MEROPS" id="M41.A10"/>
    <dbReference type="GeneID" id="10540662"/>
    <dbReference type="KEGG" id="pgr:PGTG_12945"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12945"/>
    <dbReference type="HOGENOM" id="CLU_000688_23_0_1"/>
    <dbReference type="InParanoid" id="E3KQI8"/>
    <dbReference type="OMA" id="ARQKGNF"/>
    <dbReference type="OrthoDB" id="1413014at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005745">
        <property type="term" value="C:m-AAA complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097002">
        <property type="term" value="C:mitochondrial inner boundary membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140567">
        <property type="term" value="F:membrane protein dislocase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034982">
        <property type="term" value="P:mitochondrial protein processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065003">
        <property type="term" value="P:protein-containing complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd19501">
        <property type="entry name" value="RecA-like_FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000019">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.760:FF:000003">
        <property type="entry name" value="AFG3-like AAA ATPase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000001">
        <property type="entry name" value="ATP-dependent zinc metalloprotease FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1690.20:FF:000003">
        <property type="entry name" value="Mitochondrial inner membrane AAA protease Yta12, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1690.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.760">
        <property type="entry name" value="Peptidase M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01458">
        <property type="entry name" value="FtsH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050928">
        <property type="entry name" value="ATP-dep_Zn_Metalloprotease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005936">
        <property type="entry name" value="FtsH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000642">
        <property type="entry name" value="Peptidase_M41"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037219">
        <property type="entry name" value="Peptidase_M41-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655:SF2">
        <property type="entry name" value="AFG3 LIKE MATRIX AAA PEPTIDASE SUBUNIT 2, ISOFORM A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43655">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01434">
        <property type="entry name" value="Peptidase_M41"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140990">
        <property type="entry name" value="FtsH protease domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="14" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="18">
        <location>
            <begin position="366"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="37"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="809"/>
            <end position="888"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="37"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="17">
        <location>
            <begin position="107"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="17">
        <location>
            <begin position="126"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EFP86563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="888" mass="96292" checksum="8020B66A7E38098C" modified="2011-01-11" version="1">MSIRRIPNLILNHPHHLRTQFLNNRYPNRLGLFQSISSSTRSSDSQPSSSSSSSPSPSNSEKPRNPRRNSTKNQPPKKDQQQEEDQNPFSDFINLSKLFGQPSKPNSSEHPKFNNESDHGKGNKSGKGASSTSGSEQQQSPGAANGLVFWALGAYALYHILAGDSLSNREISWQEFRTGVLDKGLVEKLEVINRSKVKVYLHPNAFKLTGINPNGSSSAPSGSIASPSAGASYFFSIGSVEAFERKLDKAQDELGIPSHERVPVSYHEQASLMNLFWNFAPTLTLAGLLFYVSRRATGMGGAGGGAGGPGGIFNIGKSKAKMFNHESEVKTKFKDVAGMDEAKEEIMEFVKFLKEPEKYERLGAKIPKGAMISGPPGTGKTLLAKATAGEAGVPFLSVSGSEFVEMFVGVGPSRVRDLFATAKKNSPCIVFVDEIDAIGKARGKSGAMGGNDERESTLNQLLVEMDGFDTSQHVVVLAGTNRADVLDKALLRPGRFDRHIAVDRPDVSGRRQIFLVHLRPLVLEGAIKTKEDLKSSGSDPLPEEDEDFEGPESVEKVIPDWLVKLSHKLAAHTPGFSGADIANVCNEAALIAARLSAEFVTEKHFDMAIERVVAGLERRSRVLSPEEKRTVAYHEAGHAIMGWFLEHADPLLKVSIIPRGVGALGYASYLPQERFLYTTEQLIDRMCMTFGGRVAEEIFFGKITTGAQDDLQKITKLAFELVGNYGMSRDFGPISFGRSDSQQESFQKPYSEKTGEHLDSTVRALINQAHKRTTELLTEKKELVDKVAQRLLDREVLSRQDMIDLIGRRPFDRPDAYDDAMGSSGKPGPPPAPGSPSGGQNRPTPKPATDGPNPLGEGIEGGGGVPLPTPALSSPAVLPEQSHSGLAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3KR00</accession>
    <name>E3KR00_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13107</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178302">
        <property type="protein sequence ID" value="EFP86725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331144.1">
        <property type="nucleotide sequence ID" value="XM_003331096.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KR00"/>
    <dbReference type="STRING" id="418459.E3KR00"/>
    <dbReference type="GeneID" id="10541149"/>
    <dbReference type="KEGG" id="pgr:PGTG_13107"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13107"/>
    <dbReference type="HOGENOM" id="CLU_1563648_0_0_1"/>
    <dbReference type="InParanoid" id="E3KR00"/>
    <dbReference type="OrthoDB" id="1929172at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="171" mass="18747" checksum="07EFE8D865DCB775" modified="2011-01-11" version="1">MSALLINEKYQASLGQKTYDYRELIGLIDWANALYVPDNVYDDRKRMASKRASLGVQIGSFSSSNRIVLSGTRAGVLAACGYLNELEIAHRAANFPCSPILVTRNSSLSSPSTSTSSFSLGSTGSTERWKRRKTDSGEAVMQTAYISTLNEDLESLKTQFSDSICRPVCQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3KRP7</accession>
    <name>E3KRP7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NADH dehydrogenase (Ubiquinone) 1 alpha subcomplex 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12713</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178303">
        <property type="protein sequence ID" value="EFP86972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331391.1">
        <property type="nucleotide sequence ID" value="XM_003331343.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KRP7"/>
    <dbReference type="STRING" id="418459.E3KRP7"/>
    <dbReference type="GeneID" id="10541886"/>
    <dbReference type="KEGG" id="pgr:PGTG_12713"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12713"/>
    <dbReference type="eggNOG" id="KOG2865">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_007383_6_4_1"/>
    <dbReference type="InParanoid" id="E3KRP7"/>
    <dbReference type="OMA" id="PEDQFTN"/>
    <dbReference type="OrthoDB" id="275457at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044877">
        <property type="term" value="F:protein-containing complex binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006744">
        <property type="term" value="P:ubiquinone biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05271">
        <property type="entry name" value="NDUFA9_like_SDR_a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051207">
        <property type="entry name" value="ComplexI_NDUFA9_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126:SF11">
        <property type="entry name" value="NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 9, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12126">
        <property type="entry name" value="NADH-UBIQUINONE OXIDOREDUCTASE 39 KDA SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13460">
        <property type="entry name" value="NAD_binding_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="1">
        <location>
            <begin position="65"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="386" mass="43487" checksum="F834C23E78439208" modified="2011-01-11" version="1">MGSGYLTPSLLGNLRHQVRKQSSAISETKRWRHDLIKQSGTGKVIQSSGPSGRHSNTGLVATVFGATGFLGRYLVHKLAKQGTQVIVPYRDEDSKRHLRVMGDLGQIVPLEFDLENEGFINECVRHSHVVYNLLGREHETKNYSFDRLFIEGASKITRIAKENGVGRLIHVSHLNASLDSPSKLYKAKAAGESVVRDIFPEATIVKPSIMFGQEDRFLNKMVTKPFTFQVNRLETLVRPVSVLDVAEAMQLMSTADSTAGKTFELAGPRTYTHEELFQVLEVLTHKSLTPSFINLPLPIFKFLMKIVDKAVWWPTLSEDEIVRRCIDDKLELTKGCYGFDYFGIEPDAVENLAITYLRYYRTASLFDAPIERGGLKVKPRTYHAVQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KRY4</accession>
    <name>E3KRY4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_13278</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EFP87059.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331478.2">
        <property type="nucleotide sequence ID" value="XM_003331430.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KRY4"/>
    <dbReference type="GeneID" id="10542140"/>
    <dbReference type="KEGG" id="pgr:PGTG_13278"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13278"/>
    <dbReference type="HOGENOM" id="CLU_572555_0_0_1"/>
    <dbReference type="InParanoid" id="E3KRY4"/>
    <dbReference type="OrthoDB" id="2507194at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173838" evidence="2">
        <location>
            <begin position="19"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="140"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="181"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="218"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP87059.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="477" mass="53988" checksum="03D6663C9F116B85" modified="2012-04-18" version="2">MAPSKLAFLVLLISIMESAHEIGAIPHPSFKDARFVDSAHVPVQMPTVQMHDVEKPLAERVSPSLQGPLEREQFHDRQIHESIPSQDDPASFMVCIQPEAKFSHVRRGAHAFRYAGFIINDSQVKVIKSTPRSNNDEDLIPSRREHTESSLRWNPTPPRPLKRTRFSDESVGSKRQPRWSAENNGLPTESNIRWTKSSSIPSHHDHENLPIEKQAGVSSSSGKGESDSTTSRSMGQDPVESSTVQKMDLPVKQPLKKQDWMTGQISFFSSEYSKNLHTIYLRLGKLYDSSNTVRTISKKRMIGVPVVMTSIQMGESPLTLLNWIVKTDGNHCPVKELISKFKHLANFITCYHSELLKALGIDVSEFGTLNEALLKWVLNEIFNPSTGFPIIGMITDQKLVQGLGKEESPFRDLDAYLSIYFSRPEADQDSSKDACVLINYWYRSQQTQIHKKFSGNDKDHFMHLLKKIHMDFSVPTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3KS38</accession>
    <name>E3KS38_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_13332</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EFP87113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331532.1">
        <property type="nucleotide sequence ID" value="XM_003331484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KS38"/>
    <dbReference type="STRING" id="418459.E3KS38"/>
    <dbReference type="GeneID" id="10542355"/>
    <dbReference type="KEGG" id="pgr:PGTG_13332"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13332"/>
    <dbReference type="HOGENOM" id="CLU_012390_7_0_1"/>
    <dbReference type="InParanoid" id="E3KS38"/>
    <dbReference type="OMA" id="WSRIDAY"/>
    <dbReference type="OrthoDB" id="2506822at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125">
        <property type="entry name" value="F21J9.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125:SF3">
        <property type="entry name" value="NO-APICAL-MERISTEM-ASSOCIATED CARBOXY-TERMINAL DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="2">
        <location>
            <begin position="181"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="12"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="37"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="216"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP87113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="264" mass="28958" checksum="781462E158AFF396" modified="2011-01-11" version="1">MAPKKTKKSAEDPPPSSTPTPTTPSSPKKKINKNKSKPTPTSQSTNTATTPIPADKSNNNKDATPKADTNPTPKKGFPQWSIEEDKKLCIAWLNTSRDAIVGKGQKATTFWERIHATLTELITEYNEEKNHSRNFKPLPLRPVGAVECRWGLILKSVNKFAGFYSNLSGAKELYKTTLGLPFNLDHCWGILKDTPKWQATQRENEARTKKTTKSETAAPSTDASSSVVAVSSPWVVDIEDNDSGPSRSVLGNGRVKGQKAAKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>E3KS78</accession>
    <name>E3KS78_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">P-loop containing nucleoside triphosphate hydrolase protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_13372</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the GLYK kinase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EFP87153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331572.1">
        <property type="nucleotide sequence ID" value="XM_003331524.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KS78"/>
    <dbReference type="FunCoup" id="E3KS78">
        <property type="interactions" value="410"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KS78"/>
    <dbReference type="GeneID" id="10542396"/>
    <dbReference type="KEGG" id="pgr:PGTG_13372"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13372"/>
    <dbReference type="eggNOG" id="KOG2878">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056986_1_0_1"/>
    <dbReference type="InParanoid" id="E3KS78"/>
    <dbReference type="OMA" id="FWRSLHP"/>
    <dbReference type="OrthoDB" id="347435at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001691">
        <property type="entry name" value="Probable ATP-dependent kinase TDA10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10285">
        <property type="entry name" value="URIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00061312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP87153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="340" mass="38724" checksum="8F0925EF55ACF2D1" modified="2011-01-11" version="1">MVLAEPWKTGRTTIQRTDQKLSGTTRGTAKEILVQFILTQLRSHPTRVTEAGVHKPMIIGLQGPQGIGKTWLSREVKEELNKVHHINTIALSLDDLYLPRRAQEKLEEEDAGGRNRLLQGRGLPGTHDLDLAVRVFDSLSKINETKEIFLPVYDKSALHGLGDRIGFNTLSVPSDHVLQLVIFEGWMLGFVPLDPLQLRRTYDHLLQLNPTPSSNTFITKFSIDELLKINLNLSAYVQLLWPRIDVLINLVPQDLNFIWRWRIQQENEMKAKNGGIGMSEEEVKNFIFRYMPCYELYGQSVDDPDGKGMIPASLKVVMDEERKVVDVLYPKESTADTEIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KSC0</accession>
    <name>E3KSC0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_12790</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178305">
        <property type="protein sequence ID" value="EFP87206.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331625.2">
        <property type="nucleotide sequence ID" value="XM_003331577.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KSC0"/>
    <dbReference type="STRING" id="418459.E3KSC0"/>
    <dbReference type="GeneID" id="10537437"/>
    <dbReference type="KEGG" id="pgr:PGTG_12790"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12790"/>
    <dbReference type="eggNOG" id="ENOG502SBYH">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004591_1_0_1"/>
    <dbReference type="InParanoid" id="E3KSC0"/>
    <dbReference type="OrthoDB" id="2246127at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP87206.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1012" mass="114208" checksum="081CB974AEA72AE3" modified="2012-04-18" version="2">MVVFALWVQEDESRLSPHENKNSAGLEVDDPALDVSNYQTTQSWTACEDLATNGKDNHDFMDAGELNTEDNAFSDHELDSCASDLDTSSDLSDEANSKDFELSAEDLDLMRELGRVGDGDLASTTPRPHVDRSWWPFRTKEVCLQLIPVHDACLFYIFCQYMIASLVIGYAHAILSQSIYDRIRSMFSLVHVILPDWTTNRREKKKHRDLLQMDVKKSYSILGNPTYALSIQKILAQEVGNPLVTQAMDYYPEDARGKNIFKLSQSKKWLEDMDPDSRAPMVRTSNGDFYLFEPVQLSNDSVVVPIFFYKRDVAHEINISEFYCAFPNIAGPDGLMLSQRCQGRLYELGDHMTLIHLLPNPWRNHAKGRMIRHMPITLYCDDTSGNQSKRWNKHVPFYFTLSGLPPNWSNQQYNCHYLITSNVANVMELAGPIVDEMNQLSTTRAVAWDAGLTEEVLLTSNVLCFLGDSPMHAEATSTPIPANSLQAFRACDLSSVSVKDKATIDYVCSFTMLDSSGLWINNPKRSWAKIIENCYHIWHKSEKPWTKTAVGEEITKLGVRDVLNTSIIDRRYEILAKKDAATAEDVQFVQGLKNLDQGGKEALFSPFFQLKGFDGCADTPVEVLHVFLLGIVKYMLRSFMKSLTAGVLPEVMARYESFDTKGLNVPSLRPYYLTKHYSNLIGKDFKVALQAAPFVLFEYMLEDKRLVWSALCQLAPLVFQTHIADMDVYQISLRQLVRVFIYHLIKSTAQWVNKPKIHMLLHLADSILRFGPAALFATEKFESYNGVLRKSSIHSNRQSPGKDIGISFANFRNLRHLVSGGYFFNRIANTYQTASAKVLELFANSPSVQKSMGYNAKNLDNPIPFKPTVGGRRIRKANNVTIPSDLTTQLPGHAFKQLPGIHITRKDLLSVGSFVLFKSNQGALLELGQIDHMWEAQKERRAKFVVCLTPFEFGGISKFYNMRETRRGGQGRIVGIQDVVSTLNVQHNCHKGRCSIDLTNSQGLFRGTGGGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3KSC1</accession>
    <name>E3KSC1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12791</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178305">
        <property type="protein sequence ID" value="EFP87207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331626.1">
        <property type="nucleotide sequence ID" value="XM_003331578.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KSC1"/>
    <dbReference type="GeneID" id="10537438"/>
    <dbReference type="KEGG" id="pgr:PGTG_12791"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12791"/>
    <dbReference type="HOGENOM" id="CLU_041944_0_0_1"/>
    <dbReference type="InParanoid" id="E3KSC1"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004820">
        <property type="term" value="F:glycine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006426">
        <property type="term" value="P:glycyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006194">
        <property type="entry name" value="Gly-tRNA-synth_heterodimer"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50861">
        <property type="entry name" value="AA_TRNA_LIGASE_II_GLYAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="74"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="109"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="504" mass="54958" checksum="CE3FB9F077370A4F" modified="2011-01-11" version="1">MSVVVSSDSASEYQGSKQSYSPAPKTPGPRTRATTQSSNRASQTGANRATVHRSHGTSDQSPSPAPDTPGPRTRLTTQSTNREPQTGPVRPTVRRTHGAGGRLPAGPQRGASTRAAAPPGRGASRPRPANLPGVPSRARATALSTRQATAVQPNGNEADPQGAWPPATVHLTVDDEPINNEFVSEVNAIFQLGGSYADLGEQLAYVPLPRQYAVGIYGLLLVRQAIERLRHDILHAPRGPGTASAEVSMAFQAQNFHYVAVFSDYVTRQARELLVSQNLGVYSSEAPQGAPRKARGLLTLVLEKINEQSDEFKIRYLPRGYSDPDPAARGSVTTYVQGKLRNSRGKMRDLLLTNIDMESELPVSTISFLLTLMRSSLIPPLPNNAPATDTTDKGQREYGLLKARLAYLQLQTIINYIRQGGQSNRQWKNIDEHLRDLAYKSREYRSAFYQLVTRYDRKIFGGEEVFSNIDRDSISLPTHEEIEALVASNSAVNVEAEGDPGEDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3KU19</accession>
    <name>E3KU19_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_14509</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178309">
        <property type="protein sequence ID" value="EFP87794.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332213.2">
        <property type="nucleotide sequence ID" value="XM_003332165.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KU19"/>
    <dbReference type="STRING" id="418459.E3KU19"/>
    <dbReference type="GeneID" id="10534689"/>
    <dbReference type="KEGG" id="pgr:PGTG_14509"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14509"/>
    <dbReference type="eggNOG" id="ENOG502S1MF">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_308859_0_0_1"/>
    <dbReference type="InParanoid" id="E3KU19"/>
    <dbReference type="OrthoDB" id="28755at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37490">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37490:SF1">
        <property type="entry name" value="GLYCOSYLTRANSFERASE 2-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="172"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="201"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="362"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="402"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="619"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="230"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="574"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="80"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="574"/>
            <end position="590"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP87794.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="955" mass="108593" checksum="A65B382F78CE86C8" modified="2012-04-18" version="2">MYNKLVVYTSPTNKKNKMKPANQHPPTSSPSPDYKNQELEKKEQQEQQLQLINNHRRSQSLHSTTQITSHSSNSQHNLPTLQTNHLNHHQNSTSSSSLTSSSSSSSSSTPPRHHHHQYNNSFHHHPASPIKHIHHQQQHPESLSEHHHQSSSDHLLNHHHSPRRSDQPTHSLLSNSILVISLTITSSFSSILLSISTTQLTHLSLATFPILEFLSLSIITRLPILNRIQSTYNPHQPNPSHHPHHRSRCPTPPPPPKNSNLTNSHHRSYQSVPRFTGWRLLLRFTLPAALLSLLASWSKTEIAGRVGCGTWQAFDIYALPIAVVALSRLPSIKQPRRELVATVSIITFAISLMFFAEPIYPFLIILGILSAASQALYLTHLKTWLLSFPQLNPCHALAHSSPFALLLIFVPAFANAIYQSMTRFGHLDLTPRFTFHATIWLIMYLLARVTERLLELWIVYRFQSPLSVILVVPLRNLLSVGIAEVSQFYLGRLGSAQSIACYSFSLLGLYHAERDVSQALRQFCKFIFNRCSHPKLPGSSRRRSSIYTDPDAETIQEKSPDELLHSREVDHGRLSSIERDRPRDEEDYRRQAPTSRFRKPSFSERYRSERKLKTHWQTLFRSIAFGPLIVLLLIKLIWQPPIGVPMTEMPLATARPAGSVDLVFSYYNEPLSRFFEAVDHVRRLSVLSLQNPRVIVYVKHPEVSLVNIANVINADEVIRLDNVGREGGTYLTHILKHYNSTILTDQQGRVIRSGKSDPNILEQAEFGVERVLSSPAIHGLADHTVFMQPEISWHWIAKPRMDLFDPKSTGFLSFGPYLVSVCGQDGLGNGNYERMRDIYTMFYNSFCPPTGQLASYAGQFVVSKARILSNRYEQYLNMRRLLEAPKEHWIHQEGGWLKWKGATDTAATNPTGPQAPFLGHALERSWPVIFRCFDPSIAESCPDDLNDKRLCQCFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>E3KUH8</accession>
    <name>E3KUH8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Putative nuclease HARBI1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Harbinger transposase-derived nuclease</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGTG_13732</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Transposase-derived protein that may have nuclease activity. Does not have transposase activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HARBI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178310">
        <property type="protein sequence ID" value="EFP87928.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332347.2">
        <property type="nucleotide sequence ID" value="XM_003332299.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KUH8"/>
    <dbReference type="GeneID" id="10534907"/>
    <dbReference type="KEGG" id="pgr:PGTG_13732"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13732"/>
    <dbReference type="HOGENOM" id="CLU_018552_2_0_1"/>
    <dbReference type="InParanoid" id="E3KUH8"/>
    <dbReference type="OrthoDB" id="2502344at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026103">
        <property type="entry name" value="HARBI1_animal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF85">
        <property type="entry name" value="GH03217P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02086">
        <property type="entry name" value="PUTNUCHARBI1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="14">
        <location>
            <begin position="203"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="372"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="385"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015519"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00045850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EFP87928.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="418" mass="47750" checksum="D5F433D4DEEA8C1B" modified="2012-04-18" version="2">MAYHHNEATEAILDGVMKLPSIPSFGAILFPGKPSLQFIWEMFFDDEAEVAEILEELLSHRYLSLRTTPTTHDDYDLAKLFDMRDNDFKQAVRTTKDGFLWLLDRIRHHPIFYSNSPRPQLPIPHQLALTLERLGSNGNGASVGRFSRNLSVGRGTVIKVSRRVIRAINEVSTNYIVWPNKERRAEISEVMTEEGFEGCIGFVDGTTIPLHQRPGLDGEVYWDRKKQYSINCQVICDCDRFITSFMTGWPGTCGDSLVFKNMKVHLEPDRFFDPGQYLIADSAYALSMTTIPAYKSPLSKINRNTEFNYCLAKSRVRNEHTIGILKGRWASLQQLWLSLYTPQHMVEIIRWINCCVALHNMLAHLGDSWDELATPNDNDEPGDSEGNHENNTEGHREEIQEKCLLFNHLVGTLPIPSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KUU7</accession>
    <name>E3KUU7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF908 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12604</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178311">
        <property type="protein sequence ID" value="EFP88157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332576.1">
        <property type="nucleotide sequence ID" value="XM_003332528.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KUU7"/>
    <dbReference type="STRING" id="418459.E3KUU7"/>
    <dbReference type="GeneID" id="10535380"/>
    <dbReference type="KEGG" id="pgr:PGTG_12604"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12604"/>
    <dbReference type="HOGENOM" id="CLU_2321516_0_0_1"/>
    <dbReference type="InParanoid" id="E3KUU7"/>
    <dbReference type="OrthoDB" id="10633689at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010309">
        <property type="entry name" value="E3_Ub_ligase_DUF908"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06012">
        <property type="entry name" value="DUF908"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF908" evidence="1">
        <location>
            <begin position="3"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="99" mass="11147" checksum="C4001E1FD0FB91C3" modified="2011-01-11" version="1">MASAPAPVEGLATIHISVNTIASGRWPQDILADLSKQHQLPIDFQFKPFHRIWIAMAHRNSLDVWRFAIVRLLALAIYTHTTDKTTTLSKLIINEASLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVY3</accession>
    <name>E3KVY3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PEBP-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_14036</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178314">
        <property type="protein sequence ID" value="EFP88458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332877.1">
        <property type="nucleotide sequence ID" value="XM_003332829.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KVY3"/>
    <dbReference type="FunCoup" id="E3KVY3">
        <property type="interactions" value="7"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KVY3"/>
    <dbReference type="GeneID" id="10540761"/>
    <dbReference type="KEGG" id="pgr:PGTG_14036"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14036"/>
    <dbReference type="eggNOG" id="KOG3346">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035836_1_1_1"/>
    <dbReference type="InParanoid" id="E3KVY3"/>
    <dbReference type="OMA" id="FRTQWDE"/>
    <dbReference type="OrthoDB" id="2153661at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00866">
        <property type="entry name" value="PEBP_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.1180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.280.10">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008914">
        <property type="entry name" value="PEBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036610">
        <property type="entry name" value="PEBP-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035810">
        <property type="entry name" value="PEBP_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11362:SF82">
        <property type="entry name" value="PHOSPHATIDYLETHANOLAMINE-BINDING PROTEIN 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01161">
        <property type="entry name" value="PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49777">
        <property type="entry name" value="PEBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="357"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="357"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP88458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="406" mass="46751" checksum="6FF82970EDC1DF74" modified="2011-01-11" version="1">MINRSSRLTRPIIPIKQIQSKTILIGFNKQPKNHYLSTTTNEQVKPNVTVVRGTGTKKQSSPSTSSTSIQKAFQESIKFLKEDSERLQQKLKEEQTSADSQQQPEEIERLEILSEINDVQVRKNHHLGQQIDMSKPIYRFLEQQRWRKEGQLGRLMETIHKLRIFPDVYPSINPTVNLKVKYEDTYISTEKRLINVGNFVPSSKSMDPPVIQAQVFHPEPRLYTLVMVDPDVPDPRNHSFTTFLHWLRANVSISATTNEDLDLKTMKESEEEIKYIGPHPAEGSGTHRYTIMLFEQTGPIDLKEHSDILGRPGFSLRRFSSQIGLKPAGVMAWISQWHKRDAQAISSIYRDQLQIHEPRYGKEPKPYSDRIKLPSHTPLPLTHRHPSVIPWRQKISSSNSDPTLST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>E3KW59</accession>
    <name>E3KW59_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="17">Pheromone-processing carboxypeptidase KEX1</fullName>
            <ecNumber evidence="15">3.4.16.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="18">Carboxypeptidase D</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="16">Pheromone-processing carboxypeptidase kex1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PGTG_14112</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Protease with a carboxypeptidase B-like function involved in the C-terminal processing of the lysine and arginine residues from protein precursors. Promotes cell fusion and is involved in the programmed cell death.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Preferential release of a C-terminal arginine or lysine residue.</text>
            <dbReference type="EC" id="3.4.16.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Golgi apparatus</location>
            <location evidence="2">trans-Golgi network membrane</location>
            <topology evidence="2">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase S10 family.</text>
    </comment>
    <dbReference type="EC" id="3.4.16.6" evidence="15"/>
    <dbReference type="EMBL" id="DS178314">
        <property type="protein sequence ID" value="EFP88534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332953.1">
        <property type="nucleotide sequence ID" value="XM_003332905.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KW59"/>
    <dbReference type="FunCoup" id="E3KW59">
        <property type="interactions" value="236"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KW59"/>
    <dbReference type="ESTHER" id="pucgt-e3kw59">
        <property type="family name" value="Carboxypeptidase_S10"/>
    </dbReference>
    <dbReference type="MEROPS" id="S10.007"/>
    <dbReference type="GeneID" id="10541000"/>
    <dbReference type="KEGG" id="pgr:PGTG_14112"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14112"/>
    <dbReference type="eggNOG" id="KOG1282">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008523_11_1_1"/>
    <dbReference type="InParanoid" id="E3KW59"/>
    <dbReference type="OMA" id="NTYNPCV"/>
    <dbReference type="OrthoDB" id="443318at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004185">
        <property type="term" value="F:serine-type carboxypeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006915">
        <property type="term" value="P:apoptotic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000121">
        <property type="entry name" value="Carboxypeptidase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001563">
        <property type="entry name" value="Peptidase_S10"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802:SF190">
        <property type="entry name" value="PHEROMONE-PROCESSING CARBOXYPEPTIDASE KEX1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11802">
        <property type="entry name" value="SERINE PROTEASE FAMILY S10 SERINE CARBOXYPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00450">
        <property type="entry name" value="Peptidase_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00724">
        <property type="entry name" value="CRBOXYPTASEC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0053">Apoptosis</keyword>
    <keyword evidence="4" id="KW-0121">Carboxypeptidase</keyword>
    <keyword evidence="13" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="11" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 20" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <keyword evidence="6 20" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 20" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="20">
        <location>
            <begin position="527"/>
            <end position="545"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="552"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="19">
        <location>
            <begin position="620"/>
            <end position="668"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="19">
        <location>
            <begin position="552"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="19">
        <location>
            <begin position="627"/>
            <end position="638"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00037042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00038895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00040403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00040628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00042717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="EFP88534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="668" mass="73837" checksum="A4A383FB29F28643" modified="2011-01-11" version="1">MSTTTNDDSSSELPSSADFLIDALPGQPKSTTQSIIQYAGHLPSHPPSTLSPPPDSHLWFWLIRSKHIADREKFVIWFNGGPGCSSAEGALMEIGPLTPSLKNEPGKPIELVESTIGWSEYANLLFIDQPAGTGYSYVNVGDDVRELAGASEQVVVFLKNFYKVFPEFSKIDTYLAGESFAGQYIPYFAQAILDTAALSTPLLGLMMGNPWINPKVQYLSYLDFAYERGMIVKGTSSAVEAEKSFQKCIKALKGKTESQRILVDSCEEGLQSILDAGAQVVNGTKMTPVPYNITEMSAEESVEWPKKNKNLKAYLVQNSTVSALHATAKTGKWTLCNDTIGKEMWVPRSAPSSTLLPALVKKLKVIVYVGDQDYMCNSLGLNRSISEMEWNGQKGWGVDDKGQQIVPQDYYVNGTKQGTWTEARGLTFVKYDRASHLVPSDAPHAAHDMLLRFMGVDALLAAGPGGSLSSQVGTDDHKGIIVKTHPNGTLVDQDNLITDLDKKPSIGAAPILETIEERQAYYGPRQTIALIVLLTGLSLGLVMLFKWRRRRRRARHGSKNKGRAISMQSRSPEAYGHRNTLLRRSPSPTQRKQSRHPTRKLNGNHLTLDEEAQALVHAEETSNLEPTTLSRRTSLFSSDVPSHSRHESQIFSLEDEEEEDDDHHHQNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>E3KW78</accession>
    <name>E3KW78_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">D-xylose 1-dehydrogenase (NADP(+), D-xylono-1,5-lactone-forming)</fullName>
            <ecNumber evidence="3">1.1.1.179</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">D-xylose-NADP dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_14758</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>D-xylose + NADP(+) = D-xylono-1,5-lactone + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22000"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15867"/>
            <dbReference type="ChEBI" id="CHEBI:53455"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.179"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the Gfo/Idh/MocA family.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.179" evidence="3"/>
    <dbReference type="EMBL" id="DS178315">
        <property type="protein sequence ID" value="EFP88553.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332972.2">
        <property type="nucleotide sequence ID" value="XM_003332924.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KW78"/>
    <dbReference type="FunCoup" id="E3KW78">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KW78"/>
    <dbReference type="GeneID" id="10541024"/>
    <dbReference type="KEGG" id="pgr:PGTG_14758"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14758"/>
    <dbReference type="HOGENOM" id="CLU_023194_7_2_1"/>
    <dbReference type="InParanoid" id="E3KW78"/>
    <dbReference type="OrthoDB" id="2129491at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047837">
        <property type="term" value="F:D-xylose 1-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000683">
        <property type="entry name" value="Gfo/Idh/MocA-like_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050984">
        <property type="entry name" value="Gfo/Idh/MocA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055170">
        <property type="entry name" value="GFO_IDH_MocA-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22604">
        <property type="entry name" value="OXIDOREDUCTASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22604:SF105">
        <property type="entry name" value="TRANS-1,2-DIHYDROBENZENE-1,2-DIOL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01408">
        <property type="entry name" value="GFO_IDH_MocA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22725">
        <property type="entry name" value="GFO_IDH_MocA_C3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gfo/Idh/MocA-like oxidoreductase N-terminal" evidence="7">
        <location>
            <begin position="9"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="GFO/IDH/MocA-like oxidoreductase" evidence="8">
        <location>
            <begin position="152"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="219"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00042988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP88553.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="408" mass="45225" checksum="DF3F29731DD33E66" modified="2012-04-18" version="2">MVREQFTCRWGIMGAGAISEKFVRDLLIDPKTREVSDVHHRIAAIGSRSFVKAEDFISRIEGLESHSTKAYGSYQDLVSDLEVDVIYVGTPHSSHFSDVMLCLGSNRNVLCEKPMTVNAAQARELCRAAKEKQCFLMEALWTRFLPITHSFQKVIASGAIGEIQRVDADLSVDFAPDSLPNDNRLVNPKLAGGALLDLGPYPWTMLALTLLHQNIKPESSSFHDNKHENPAPTDLSIPKISASGTMYTHRNAHPSSEPVDGSVIAVLEFPTPSGRKAQGLLISSMLQNTNEDRAVTIYGTKGRIRIPSLACCPYQFGVTVYPDQAEGNALPTSQGRDNHPQEKIQSFEIPGNAKGYMWEADEVARSIASRKLESKRMPHSESILMMEVFDEIRRQIGLKYPNSIESMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KWL0</accession>
    <name>E3KWL0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14643</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178316">
        <property type="protein sequence ID" value="EFP88677.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333096.2">
        <property type="nucleotide sequence ID" value="XM_003333048.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KWL0"/>
    <dbReference type="GeneID" id="10541466"/>
    <dbReference type="KEGG" id="pgr:PGTG_14643"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14643"/>
    <dbReference type="HOGENOM" id="CLU_2016328_0_0_1"/>
    <dbReference type="InParanoid" id="E3KWL0"/>
    <dbReference type="OrthoDB" id="10336938at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88677.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="123" mass="13235" checksum="FCFFDCDB20837A0E" modified="2012-04-18" version="2">MQVDGVAADVEPTRHETTPAVKEVKPKKKRFMMSPLKKSFSPNNSQDGNSTDDSAHAKPKKNQPLAGRFKHVLCQVKSKVTMLLLPGENESMTPPAAPLEDLTVSCVALPMDAALPNDNAVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3KX90</accession>
    <name>E3KX90_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_15166</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178318">
        <property type="protein sequence ID" value="EFP88963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333382.1">
        <property type="nucleotide sequence ID" value="XM_003333334.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KX90"/>
    <dbReference type="STRING" id="418459.E3KX90"/>
    <dbReference type="GeneID" id="10542404"/>
    <dbReference type="KEGG" id="pgr:PGTG_15166"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15166"/>
    <dbReference type="HOGENOM" id="CLU_055324_1_1_1"/>
    <dbReference type="InParanoid" id="E3KX90"/>
    <dbReference type="OMA" id="GWARINE"/>
    <dbReference type="OrthoDB" id="76487at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45023:SF4">
        <property type="entry name" value="GLYCINE-RICH PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="2">
        <location>
            <begin position="108"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="276"/>
            <end position="335"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="160"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP88963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="335" mass="37794" checksum="06021D8A77D17E4C" modified="2011-01-11" version="1">MPTRSANWLPTEDQQLAKSWLKISEDPIISTGQKRAEFFKRVAEDYNDYSGGIERDGTMAMHRWGHIQKACLKFSGIYHKLKNNRPSGSVLSDLLPDTKKAFYEQEGKQFIFELAWMVVKDSSKFNSLAQTRGDQSKQATTPAPTPTPSTPAAQSDAALGNSQPEDIAQFGSNNSWNRPAGIHATKRAMKQEHFNAKKIKVMSEQSSNYRERTIAMKKTNEIRQQVAKAEVNQVNMEIMSRKEDELPDDVSREFLRLQKGEILEDLREQIRERQKLAAAKEKQTEASTNEALAASSERDSTFEDDPSSNAEDSQYIGDEDEDDIADLDPLLDTLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KXR2</accession>
    <name>E3KXR2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14965</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178319">
        <property type="protein sequence ID" value="EFP89124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333543.1">
        <property type="nucleotide sequence ID" value="XM_003333495.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KXR2"/>
    <dbReference type="GeneID" id="10546675"/>
    <dbReference type="KEGG" id="pgr:PGTG_14965"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14965"/>
    <dbReference type="HOGENOM" id="CLU_479076_0_0_1"/>
    <dbReference type="InParanoid" id="E3KXR2"/>
    <dbReference type="OrthoDB" id="2500414at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="23"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="385"/>
            <end position="407"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="569" mass="62768" checksum="1410B0D4706086D3" modified="2011-01-11" version="1">MCWASARRPRGVAGGHETRAVDNQRSAAVFPPPTTPHDRLAPQAQQNNPIDPMADPQVLSMPTKWVCLIDGGQDDDFEGFFSLIEQFTGRKMVPVPAHMLQLIGLVVHPMFCLRSHVPASHADPIVVSGKKVAGRAWSFLDLFLKTSGPDNFLRCLPGYNNSKSADLEVIPKESFKNQDHQPIVRRLIDASNIWEILLTFDESSKQTISPGAWDLLDLLITAWEIDFKNQGVPEIQTENSKGSTTKGGKICFPHFLRQLGDAPAEGFRIDSRVYDIIFEPFGRNSASPDYSWGCATNSLARAQELSIRLLHLLDRLDGIQLLEPGRLREDIVIRMLGLETTDLHVFVNILPADHLLRTRLLSRFLESVTRSVPTALTGFALSNSQSSTVSLSPGRSGLTRSTSATGLRRTPSRTALKFDDRHPQSSILNSKPQVISLSSLITQLLPRPLLDIPATTTRQSSASSKLDPTVLVNAESRYFSVLSVILGSLLAIHQSKTGSEIDQDGLSLVKQGKLRSAIQTVFDSVTEALGDKLDSLDDQKTFRTLLHRKVNRTIKAFEHLASKSPPTIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KY83</accession>
    <name>E3KY83_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_15004</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178320">
        <property type="protein sequence ID" value="EFP89163.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333582.2">
        <property type="nucleotide sequence ID" value="XM_003333534.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KY83"/>
    <dbReference type="GeneID" id="10546717"/>
    <dbReference type="KEGG" id="pgr:PGTG_15004"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15004"/>
    <dbReference type="HOGENOM" id="CLU_1603559_0_0_1"/>
    <dbReference type="InParanoid" id="E3KY83"/>
    <dbReference type="OrthoDB" id="2510080at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP89163.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="166" mass="18806" checksum="7390D6F55CCBFB05" modified="2012-04-18" version="2">MSAIEETRRPRFFLLAPPTTLQPLSPARIHNLTIQLNKSYCRELYQQCPCRVGPGDSCINYNGDPYTCRTLDCYIGDPNSRPDLPPYPLLLFKNCRKMLSDYNLGPPINQTHPIWYHADNQAQTLEAQIDGSGDKYLCTWTESTDPNNVRPYCDGCSSADTENSQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KYC3</accession>
    <name>E3KYC3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15493</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178321">
        <property type="protein sequence ID" value="EFP89314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333733.1">
        <property type="nucleotide sequence ID" value="XM_003333685.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KYC3"/>
    <dbReference type="GeneID" id="10547035"/>
    <dbReference type="KEGG" id="pgr:PGTG_15493"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15493"/>
    <dbReference type="HOGENOM" id="CLU_1611573_0_0_1"/>
    <dbReference type="InParanoid" id="E3KYC3"/>
    <dbReference type="OrthoDB" id="2503489at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="112"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="188" mass="20516" checksum="8F479D2FC31E1268" modified="2011-01-11" version="1">MARSGIRDSPPLKRPASSDHDMDLDDGPGPRQRPRGQTICQPRINLSERGGRPEIQTNYVQSPIVSPCPSNKALLRDLARLATVFDDVRDRRTTTSPRGQEDASFPDPPQRVDSNIIESSNPVEAANGIAGPSGFKRKIQASNPMAPSHPKHVRRTSPSYFEEAGRNSGPPQSGSHLNPTNGHSSNRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3L1A6</accession>
    <name>E3L1A6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16327</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP90301.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16327"/>
    <dbReference type="HOGENOM" id="CLU_769723_0_0_1"/>
    <dbReference type="InParanoid" id="E3L1A6"/>
    <dbReference type="OrthoDB" id="10642158at2759"/>
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            <begin position="1"/>
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        </location>
    </feature>
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            <begin position="1"/>
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        </location>
    </feature>
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            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90301.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="334" mass="37543" checksum="4078E0FDAD300769" modified="2012-04-18" version="2">MSAGTNTTTNIIPRFRARGVQSQGPSDKLHYQDIENVAQPISYPQVSEEDSDPPSPPHYNHDESSGKEVETPDEAPVLPDAPLQTRVFKKPEAYLNLIFNLFDIGPWRIDQLGGQTQLALPAPNIPCGHIWHKQFRSYGRTNTVATHSLIPRTPLLLVKGAIDQMDNDWKAAFLPAGYLDNNHDDLVLVNNLIRELLKYEKIDLAKLIMLGARKGRQTKTDAVPSLDRLVIQSPMRVRTGFLLLHMYIQRQQDRTTSKVKSPWEGIDRHLEILQSKSQDYKVAYNKQLFNGIKTATDIAGVEVSLPSEEEVQEMLISGAQAISKEENEADGDLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3L1F7</accession>
    <name>E3L1F7_PUCGT</name>
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        <recommendedName>
            <fullName evidence="5">Hydrophobin</fullName>
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    </protein>
    <gene>
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    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178332">
        <property type="protein sequence ID" value="EFP90382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334801.1">
        <property type="nucleotide sequence ID" value="XM_003334753.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L1F7"/>
    <dbReference type="GeneID" id="10530276"/>
    <dbReference type="KEGG" id="pgr:PGTG_16142"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16142"/>
    <dbReference type="HOGENOM" id="CLU_1627868_0_0_1"/>
    <dbReference type="InParanoid" id="E3L1F7"/>
    <dbReference type="OrthoDB" id="10329793at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
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    <feature type="chain" id="PRO_5003172379" description="Hydrophobin" evidence="2">
        <location>
            <begin position="21"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="114"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="135"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP90382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="163" mass="16974" checksum="A1EEEEC06674C25E" modified="2011-01-11" version="1">MLFPKKLFLLASLYCSSISARSLQKRLLKSGKCDLKITAENNVFASLDKNLAKVVQPRIEGNKFLKNLCGALNQANCGNVDKKDVDACNNLIKKATTDALGAAIATKWNKECCSQGGGGGQQKKKQPGNGAQKKNGGGGGGGGNPKNGGNNGGQTQKKKGNPP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3L249</accession>
    <name>E3L249_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
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    </protein>
    <gene>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
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    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178335">
        <property type="protein sequence ID" value="EFP90624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335043.1">
        <property type="nucleotide sequence ID" value="XM_003334995.1"/>
    </dbReference>
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    <dbReference type="GeneID" id="10530524"/>
    <dbReference type="KEGG" id="pgr:PGTG_16650"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16650"/>
    <dbReference type="HOGENOM" id="CLU_1723283_0_0_1"/>
    <dbReference type="InParanoid" id="E3L249"/>
    <dbReference type="OrthoDB" id="10586728at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="152" mass="16497" checksum="05EF0D1890C48223" modified="2011-01-11" version="1">MGRGGIAHANCSWQWRTSRRSGTLDLEGRLNLGSQTDGKSSSDSMPSNLARQSKSTTGELNLFLPMIYTSPRHRTQKTGNLSTLLPPFTMRLTVALLLLLATPITLLEIKLRTTNWAQILTASCGSESGQSARPTNVLYSSKCDATVGEEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>E3L2I0</accession>
    <name>E3L2I0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">C3H1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_16839</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178337">
        <property type="protein sequence ID" value="EFP90813.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335232.2">
        <property type="nucleotide sequence ID" value="XM_003335184.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L2I0"/>
    <dbReference type="STRING" id="418459.E3L2I0"/>
    <dbReference type="GeneID" id="10530717"/>
    <dbReference type="KEGG" id="pgr:PGTG_16839"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16839"/>
    <dbReference type="eggNOG" id="KOG0845">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_509095_0_0_1"/>
    <dbReference type="InParanoid" id="E3L2I0"/>
    <dbReference type="OrthoDB" id="20729at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051767">
        <property type="entry name" value="Nucleoporin_NUP42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000571">
        <property type="entry name" value="Znf_CCCH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46527:SF1">
        <property type="entry name" value="NUCLEOPORIN NUP42"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46527">
        <property type="entry name" value="NUCLEOPORIN-LIKE PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00642">
        <property type="entry name" value="zf-CCCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50103">
        <property type="entry name" value="ZF_C3H1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C3H1-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C3H1-type" evidence="3">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="140"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="267"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="355"/>
            <end position="410"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="431"/>
            <end position="477"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="490"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="276"/>
            <end position="315"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="355"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="431"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="454"/>
            <end position="468"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="518"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="541"/>
            <end position="550"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP90813.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="603" mass="61066" checksum="ABFBDDBB60591F56" modified="2012-04-18" version="2">MVVCTYFLKGSCKFGNTCRNSHVVDGANKTANTSALPGPDSFETISRTMQDDLTTERPLYYLSVYSPHKSAINMIDGTDVSPEELRFQAYQARANNTTSQYVAHESQLLQQMDSVIKTVTQNCKESYKHFYHTQLPLNHPSSTFRKPTPGVTSFGGSGFGSNNQPTAFGQAGSGFGGPSTGAFGAFGSQAANPTVSAFGQPSSVRPTISAFGQQTSAFATQPSAQPGTATAFGKPSAFGSPAPVASAFGAPSAPSAFGQPSQFGTASAFGAASSQPTTAFGTSPQPTGATAFGSTPQPTGTNAFGTNTQSTGTTAFGTPAQPVAATAFGAVSQPSGATAFGSNVQSAPTNSAGAFGQTSLNNTSTAFGQPANQATSFGQSSAFGQPQTPNAFGKPSAFGQSAFGKPSAFGQSPFGQSAAFGTPVANPSATPAATTAFGQSSGFGGSSAPFSQATPSMTTPPNNQANTSSGGGGGGFAAFAGAANPSMATSFTNNQAATTPPGPAEDSKPIVSAFGQPMTPSKPSALPNTSSFFGSANQVASSSNPNPSSSVGDRAPEKFKLLDPFWQDAPTEADLSPALLEAFRAEFFAWDFIPDVPPPITLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3L2R6</accession>
    <name>E3L2R6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Golgi to ER traffic-protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17031</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178338">
        <property type="protein sequence ID" value="EFP90832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335251.1">
        <property type="nucleotide sequence ID" value="XM_003335203.1"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L2R6"/>
    <dbReference type="GeneID" id="10530739"/>
    <dbReference type="KEGG" id="pgr:PGTG_17031"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17031"/>
    <dbReference type="HOGENOM" id="CLU_002667_0_0_1"/>
    <dbReference type="InParanoid" id="E3L2R6"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053094">
        <property type="entry name" value="Zinc_metalloprotease_ZmpB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193:SF2">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48193">
        <property type="entry name" value="ZINC METALLOPROTEASE ZMPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="208"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="392"/>
            <end position="439"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="482"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1270"/>
            <end position="1435"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="50"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="134"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="149"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="180"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="482"/>
            <end position="494"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="501"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="531"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="564"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="706"/>
            <end position="720"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="721"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="739"/>
            <end position="754"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="771"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1288"/>
            <end position="1299"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1300"/>
            <end position="1321"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1345"/>
            <end position="1357"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1381"/>
            <end position="1394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1402"/>
            <end position="1424"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1435" mass="155596" checksum="20DA4C7A929E20F6" modified="2011-01-11" version="1">MAHDTSNASSPHSNPLPADHGPPFSLPEDIPSSISEGNKAMEEGLPFKHPQSTSKDLQEKTSSSAASGEKTIKRPEEWKVPPPKRLARLVNAHQKIVSPSVQRLDKPGKSKKPTADQFRSTGRDGAALDPALSADEEDVNVADEEQTEARSSALVNQIKSLIKVTPKNAPKKSKSKKSVHNSTGPSSLVSMEIDQPVSDNRPTKLATIQGHKKVSGGPSNSIDKSDKMHVDPPALVNLKKTFAMTAAVPANQTSAPGVTPSTPAPMGHLSKDQAALSRRFPQKTPVNTGHLTRTPSTSTTISNAVLPNLAKASLAEMKAMRGIWIRDKDNTAANVSELRREGYHLLAPAAYEYQDQHLRRVTRFCSQLDRKIEESERAGVVEEIEVIDLTEEPDDPTETTVVGSTGAKRPIEGSTSMSVDDSPVESKKARMSGSAPVKIDPPNLGPYGFRAISDTEAALAGLTPRFPVNKQPSLSEFISQSNPSLMLPLSGNTSKPPPQPTTGNLRSAAASSGLTGPEISNSSSAQGVAGSSPSLVLPSTSVSAPAPSSLVPPSTSNVLDSAASGASTSKNASQPSLVSKSQSKPVGSAQRVTRGSLRTSQPTAASKSKSSEKISNTTTPQPTNDIPPASKDVPPATNADPQATNEVAPASTDVPPATNDVPPATKDLPANSTLDDSAQQSQGGQSTNAPSALPPSGRVLPNSTLPEKKKDAQNKDKDVVDEGVDELEELSDKEEAPKKRGGKGKGKAQKKTPKTPKPPKTPRLKVSEMTPEQKLERDRKVKQRKEEKDNCVYAIPPQPGALLAPMWPPLTCEEARIAHKDMLVKSRNPHWNYGPELMDRVAKIVKAFGDDFEDDEFYTKMDNFKPDLEAIQAFGLVYKDEFLNLIPTCPRLVQVDAADPFFKEGVVDLSMIKAAHDSDDKVKASSWQCLYGMMIRTDEHSDYTEDPHTVKAIDSSFRKIHTLTQSCFDHFHHYVLPSNKPVCDTSATWKQDTMFQSGEFVLAKIKALKAGAQGTASHGNKRTGNGLMILQKRIWETLFCCLMMQQALFLDDLDHCIKTKSFPNKTMVVSQYMTAEDRSKSLFKTGDVKDLKDSKALIDWGQDRLAAFGSMAIFFLYGAAGWWQCLTDSHNYNQKDVWALVYIAEAKADWLYNNGHYHERTPQDTPWYRIDGFVRWLLHKTNMHLRLTATTDTVDWQAAPRFWNQHVTEQTIARLALQDILAEVCSKSPRKSLNFNGEAAPDVVVDKEDKPLVDEFRSTLTCGWNATIRANEDAMTSQPGPGEDEGEANGGGEGEANGGGEDEEAANGGGEDEEDPNETQEEGGFIRPSHSGRSENSFATRRGGSPSEASSSSSSDESQVKVKLTQTKGRIRHRKRSLVPSSSSDDSSSSSGSSGEDEEETDIARSDTDGGLEERSRRRAEHPMIEYSAGVSRKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>E3L4T1</accession>
    <name>E3L4T1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="7">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_17610</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
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    <dbReference type="EMBL" id="DS178348">
        <property type="protein sequence ID" value="EFP91556.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335975.2">
        <property type="nucleotide sequence ID" value="XM_003335927.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L4T1"/>
    <dbReference type="STRING" id="418459.E3L4T1"/>
    <dbReference type="GeneID" id="10531493"/>
    <dbReference type="KEGG" id="pgr:PGTG_17610"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17610"/>
    <dbReference type="HOGENOM" id="CLU_011478_1_1_1"/>
    <dbReference type="InParanoid" id="E3L4T1"/>
    <dbReference type="OrthoDB" id="2502526at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="54"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="10">
        <location>
            <begin position="267"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="627"/>
            <end position="692"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="708"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="644"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="678"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="708"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP91556.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="731" mass="81976" checksum="3EC036E1E9116A09" modified="2012-04-18" version="2">MHQLAMAKRVRTPTGVNLYKKINEKTDEKLKEFIKQSSMDTYQQEPKPLQVDTVFNLARGRNTFLLAGTGFGKSRISEMYFKMIPKLKRAVILVLNPLDSLGDNQVLEKEKAGFTAINLTKMNFNKKTADSIMNGDYNFIYLSPEIFLNSKSFDEVYFSTKFQNRLALIVIDEAHMVYIWGLVESSTSKTINSVHLRIEDYALFRPCYGKLGPHLLFRNNKPVLLLSATCRPVAVEGIMKSLKLNDDNLEILRGELTRPEIRIIRVTMDHSLASTLDLVKLFPSSKDVSDEDLVPTLIYSSSRNRTLTAMDVIDLARETPGAAFDPPSSCIRRFHSCTGEQDKVDCVEDFASGKFPLISCTMALGLGQNWKRVRMVVHMGRGDPASICQMIGRCGRDGRPGLAVMFVEKNRRNGKNTVDQFTPAAYQSDPDRMDALAITPVCLRIAFSMDNLLGYIPVSVDDPAYVREWKREIEAGMSPCACSNCASTEAANLLDNLVFANKSNFDSIITDQFVTTEARDISAKYPTKQAQLRKRKIPDCDRPVFDAFKARLIADLHEYYIAQVGSSVLPAVNIFGDEEAEGIVSYMHHINKPNDIRGLIGGECFDGQLDWLFDRVSTFKSDVIDEMRSVPPKKPRPSLGVDISPSGSTAAPSSSTPMQSSSQVVNRPPTKRALAAEASRRRSLERKAQNELWLISEQKRKEQVARFLREGLQQSKDRSETRDGQPASNNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3L5R6</accession>
    <name>E3L5R6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17918</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178354">
        <property type="protein sequence ID" value="EFP91891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336310.1">
        <property type="nucleotide sequence ID" value="XM_003336262.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L5R6"/>
    <dbReference type="GeneID" id="10531838"/>
    <dbReference type="KEGG" id="pgr:PGTG_17918"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17918"/>
    <dbReference type="eggNOG" id="ENOG502S7P8">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1489709_0_0_1"/>
    <dbReference type="InParanoid" id="E3L5R6"/>
    <dbReference type="OMA" id="HSNRTWK"/>
    <dbReference type="OrthoDB" id="2507455at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="51"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="181" mass="20433" checksum="CB6DCD71F7292C15" modified="2011-01-11" version="1">MPFALPTQYSDSDDRSDYESNESNYSTDVSESTNSTDTDSDKEEESNRSQSVVSVPTSTPRHGAKEWFLPHNEDRFETFIDHGSILDKDGYPIYPNGRTVFVKVPSSKILNFGTVGYTKRTCSETPKDGEWKVTCIYCLGVLVCDQRSPGAKAKQVPAQERFTTKHVPEQWHTLMLILEPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3L5S2</accession>
    <name>E3L5S2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17924</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178354">
        <property type="protein sequence ID" value="EFP91897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="RefSeq" id="XP_003336316.1">
        <property type="nucleotide sequence ID" value="XM_003336268.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L5S2"/>
    <dbReference type="GeneID" id="10531844"/>
    <dbReference type="KEGG" id="pgr:PGTG_17924"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17924"/>
    <dbReference type="HOGENOM" id="CLU_091094_0_0_1"/>
    <dbReference type="InParanoid" id="E3L5S2"/>
    <dbReference type="OMA" id="AREDFIW"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="156"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="270" mass="29313" checksum="B47EAD30FB287E03" modified="2011-01-11" version="1">MSPTETFLEYSTRARTLQSLFNFDAAETAKLGDLQLAQFLVYGLTDALQDRINERQLLETSPFTYGPFEKQANASFLALQRPAETPALLRPTSSTTPNLSRDEFIWRVHSFLDSRGLCHFCKKHCGSVNGACPGPLNRAHVDIPANYQAPAKPVDYSAPRAWSSPSGPLSTPGKPTQPPAGRPSARAASVAGVTDVTPLEAQVSALQLDAAIREDGRWDTYFNDEGYYPSMEPAAVAALEDLDRQLLANEIEKAEQADLADAIAGHLGHA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3L657</accession>
    <name>E3L657_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_17863</name>
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    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178356">
        <property type="protein sequence ID" value="EFP92032.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336451.2">
        <property type="nucleotide sequence ID" value="XM_003336403.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L657"/>
    <dbReference type="GeneID" id="10531986"/>
    <dbReference type="KEGG" id="pgr:PGTG_17863"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17863"/>
    <dbReference type="HOGENOM" id="CLU_1960612_0_0_1"/>
    <dbReference type="InParanoid" id="E3L657"/>
    <dbReference type="OrthoDB" id="10285315at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174419" evidence="2">
        <location>
            <begin position="23"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP92032.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="138" mass="15393" checksum="0A973AB7E298C978" modified="2012-04-18" version="2">MDLCATALRVTLVLVLVSIAHAQRRARCPNTQTCGGKGIPLSEEERTQMSLKLRDLCGWEYQDGHKCKKECDKTYYSCMTCHKIFSKNTAWDDRSKLGCEHAAKRFVAIPAPSGTKPGPSEPGPSEPDNRPPVLYHFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>E3L747</accession>
    <name>E3L747_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">J domain-containing protein</fullName>
        </recommendedName>
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    <gene>
        <name evidence="8" type="ORF">PGTG_18357</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
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    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
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    <dbReference type="eggNOG" id="KOG0714">
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    <dbReference type="Proteomes" id="UP000008783">
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    <dbReference type="GO" id="GO:0005789">
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        <property type="evidence" value="ECO:0000318"/>
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    <dbReference type="PANTHER" id="PTHR43908:SF3">
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    <dbReference type="Pfam" id="PF00226">
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    </dbReference>
    <dbReference type="PRINTS" id="PR00625">
        <property type="entry name" value="JDOMAIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00271">
        <property type="entry name" value="DnaJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46565">
        <property type="entry name" value="Chaperone J-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00636">
        <property type="entry name" value="DNAJ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50076">
        <property type="entry name" value="DNAJ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="J" evidence="7">
        <location>
            <begin position="123"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="48"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="181"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="55"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="181"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="6">
        <location>
            <begin position="203"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP92370.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="481" mass="53289" checksum="5AF3FE7D9FDEC0A0" modified="2012-04-18" version="2">MEGNRDEAQRAFKLAQSLQSTDPIKSLKFAKKACALYWSPEAAALVKTLETGEGPSTTTTQNPTATSTATNTSSSSATKRSTQPSTPKPSSSNKNPTSEQTPPTYKPAQLELVKKVRRHKITQYYEILELKREADESQIKSAYRKLALALHPDKNNAPGADEAFKMVSKAFQVLSDPDKRAAYDRHGADPDSRSAGVPSSSPFGGGGGGGGGGMHFSHEDAIDPDQLFRMFFGGGGFDHPGMQFGGGPTVFQFGGGGRTTFRRAGFNRRQQQQQNPNEPEVRPASTWISLLPIILFFVVSLIQSFPSLFSSPSTPDPSFSWNPSTVYPIQRVTHTSPGVTYFVNPVEFASHPLWEQYLKANPGLSGTSETTNEEKTMEENKQTLLQDLINESRQLVEKRKANQRQYLVIPKEFKRFELEIENSWVRKLKYECQNARAHRDARRRELMGFLGIGSDWEAIKKLEAELIPACEGLKAMGYVID</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>E3L7G1</accession>
    <name>E3L7G1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Casein kinase II subunit beta</fullName>
            <shortName evidence="3">CK II beta</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_18311</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 3">Regulatory subunit of casein kinase II/CK2. As part of the kinase complex regulates the basal catalytic activity of the alpha subunit a constitutively active serine/threonine-protein kinase that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the casein kinase 2 subunit beta family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178365">
        <property type="protein sequence ID" value="EFP92486.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336905.2">
        <property type="nucleotide sequence ID" value="XM_003336857.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L7G1"/>
    <dbReference type="FunCoup" id="E3L7G1">
        <property type="interactions" value="468"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L7G1"/>
    <dbReference type="GeneID" id="10538480"/>
    <dbReference type="KEGG" id="pgr:PGTG_18311"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18311"/>
    <dbReference type="eggNOG" id="KOG3092">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_034027_3_2_1"/>
    <dbReference type="InParanoid" id="E3L7G1"/>
    <dbReference type="OrthoDB" id="3971593at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005956">
        <property type="term" value="C:protein kinase CK2 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034456">
        <property type="term" value="C:UTP-C complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019887">
        <property type="term" value="F:protein kinase regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043539">
        <property type="term" value="F:protein serine/threonine kinase activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000122">
        <property type="term" value="P:negative regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090053">
        <property type="term" value="P:positive regulation of pericentric heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090055">
        <property type="term" value="P:positive regulation of silent mating-type cassette heterochromatin formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006359">
        <property type="term" value="P:regulation of transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1820.10:FF:000003">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.20:FF:000002">
        <property type="entry name" value="Casein kinase II subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1820.10">
        <property type="entry name" value="protein kinase ck2 holoenzyme, chain C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016149">
        <property type="entry name" value="Casein_kin_II_reg-sub_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035991">
        <property type="entry name" value="Casein_kinase_II_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000704">
        <property type="entry name" value="Casein_kinase_II_reg-sub"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11740:SF39">
        <property type="entry name" value="CASEIN KINASE II SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01214">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00472">
        <property type="entry name" value="CASNKINASEII"/>
    </dbReference>
    <dbReference type="SMART" id="SM01085">
        <property type="entry name" value="CK_II_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57798">
        <property type="entry name" value="Casein kinase II beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01101">
        <property type="entry name" value="CK2_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="197"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="212"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00045899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU361268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP92486.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="275" mass="31111" checksum="882F593CF8C6D7F8" modified="2012-04-18" version="2">MTITTVDELSTGSESDYANSWVTWFLSTKGNEYFCEVDEEYILDRFNLTGLNSEVHRYGMALDLITDAFGVDELDEATRESVEGAARHLYGLIHARYIITSRGLAKMLDKYKKADFGRCPRVLCNTQPLLPVGLSDTPYSKAVKLYCPRCEDIYSPKSSRHGTIDGAYFGTTFPHMLFMVYPNMVPTKLWRLGTISNSNQGGPPPSEVGQMVGTSSNNNTPQTGSLDSALKAQRYRPRIFGFKVHQIAKRQRWQESLRDEQIDRLKKLEEQGNLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3L7T6</accession>
    <name>E3L7T6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cellulase</fullName>
            <ecNumber evidence="3">3.2.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_18610</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <reaction evidence="1">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.</text>
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        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003336982.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L7T6"/>
    <dbReference type="STRING" id="418459.E3L7T6"/>
    <dbReference type="GeneID" id="10538939"/>
    <dbReference type="KEGG" id="pgr:PGTG_18610"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18610"/>
    <dbReference type="HOGENOM" id="CLU_029718_1_1_1"/>
    <dbReference type="InParanoid" id="E3L7T6"/>
    <dbReference type="OrthoDB" id="5823761at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000124">
        <property type="entry name" value="Exported cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34142">
        <property type="entry name" value="ENDO-BETA-1,4-GLUCANASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34142:SF1">
        <property type="entry name" value="GLYCOSIDE HYDROLASE FAMILY 5 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="8 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="12">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174090" description="cellulase" evidence="12">
        <location>
            <begin position="28"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="13">
        <location>
            <begin position="80"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="362"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="378"/>
            <end position="392"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EFP92611.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="392" mass="42709" checksum="380A33628AE45273" modified="2012-04-18" version="2">MAVKQTPFWQGLFTLFSILSFFSGAFADETAPGPKPGTADSSKSAIPRLVGVSLINLPGFEFGAQTDGQYTAGTNPAMPPPESQIAHFLSQNVNFFRVPVAWEFLQPEMNGKLNEVNLKAYQTFIEKITTHGAYVAIDLHAFARYKGQIVGESPSTPASALVSLWTQLGAVFKDNPLVMFGISNEPHDLVITTWATTVQKVVTALREKKIENILLIPGTDYTAMKSFPEWYKAMKVVKNPDGSFDGLVMEVHRYLDTDNSGKSRDCTASHADEVAKAVELLKADGRQVILGETGGGSTDTCMKFLPELAAAVTDAYPVFLGFAMWAAGSFDANYELVTTVKDESSPTGWKDQGNWNSIKKFIPAKGSEKSAKSPNSKPKTKKKAACKRRAMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8R2</accession>
    <name>E3L8R2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_19029</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178376">
        <property type="protein sequence ID" value="EFP92911.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337330.2">
        <property type="nucleotide sequence ID" value="XM_003337282.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L8R2"/>
    <dbReference type="GeneID" id="10542720"/>
    <dbReference type="KEGG" id="pgr:PGTG_19029"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19029"/>
    <dbReference type="HOGENOM" id="CLU_2278808_0_0_1"/>
    <dbReference type="InParanoid" id="E3L8R2"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP92911.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="102" mass="11108" checksum="64F0B20A50EFF58C" modified="2012-04-18" version="2">MSRCISPVTGVTGSDPIGNGGVFSPPNLGFAFDSTRLIRHSILIPIAPQLERPVDPSWIQLWHDLNDLSSCIHLDRHGPVSVLQTPLVLGNTQPTPFLQSCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8X4</accession>
    <name>E3L8X4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_19012</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178378">
        <property type="protein sequence ID" value="EFP92999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337418.1">
        <property type="nucleotide sequence ID" value="XM_003337370.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L8X4"/>
    <dbReference type="GeneID" id="10542975"/>
    <dbReference type="KEGG" id="pgr:PGTG_19012"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19012"/>
    <dbReference type="HOGENOM" id="CLU_522887_0_0_1"/>
    <dbReference type="InParanoid" id="E3L8X4"/>
    <dbReference type="OMA" id="KEYTRQA"/>
    <dbReference type="OrthoDB" id="2511119at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="187"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="233"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="393"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="465"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="74"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="193"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="251"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="293"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP92999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="521" mass="56784" checksum="E2A68DD58C3F0883" modified="2011-01-11" version="1">MSDTDSDSEGHLDGLLSRHKGKGKGTSRMAGSEDDSSHQGSGSERDDPGLDFMDSLFQDSTTPPLVEEPDRNPTENNNQQAAGIQQEDLSQRFAEFAIPQEAQPSSTSLSRSLWGIFGFSTDRLEEQTELERALNEANARRDFFNASDPFHLHLRSPSKDFLIDHPIMAGALDQEHSASVGVQASLSGLIGPRPRDPHQASADPDRPDGAAVGGAVDIAAPAAHAKPFNIPVAAAGSSTDHPVPGGHSGGDNPPDVTVPTNVPSDVARCTSADTPGTAGPSADNPPPPPAEKSPVDEKIDTKPSRGDKTSSQWLGLIEVESDPEDETYLPVGKNKMKKSSDSDSELFFSLLPSEPERLNPVAGNEDQPATERLSYHALSLRNAALEDYMKELMSKTEQRIHHLNEKVNRLKRTSAQKSNAARKLKNRLKAKDKKLSELVRARQVELHAIKLLLDAATESDLHRQLRSLLDQLAQRKALIAQLQKESLLHSVLRFDLQTHRQKLESKLHQLESNPSSLINRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3L999</accession>
    <name>E3L999_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Glutamine amidotransferase type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_19229</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178382">
        <property type="protein sequence ID" value="EFP93124.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337543.2">
        <property type="nucleotide sequence ID" value="XM_003337495.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L999"/>
    <dbReference type="STRING" id="418459.E3L999"/>
    <dbReference type="GeneID" id="10543189"/>
    <dbReference type="KEGG" id="pgr:PGTG_19229"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19229"/>
    <dbReference type="HOGENOM" id="CLU_042555_4_0_1"/>
    <dbReference type="InParanoid" id="E3L999"/>
    <dbReference type="OrthoDB" id="2495291at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061672">
        <property type="term" value="C:glutathione hydrolase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008242">
        <property type="term" value="F:omega peptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006751">
        <property type="term" value="P:glutathione catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01908">
        <property type="entry name" value="YafJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000060">
        <property type="entry name" value="Related to DUG3-probable glutamine amidotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026869">
        <property type="entry name" value="EgtC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052373">
        <property type="entry name" value="Gamma-glu_amide_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43187">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE DUG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43187:SF1">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE DUG3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13230">
        <property type="entry name" value="GATase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="2">
        <location>
            <begin position="2"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP93124.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="333" mass="37928" checksum="19104F7640854033" modified="2012-04-18" version="2">MCRLLIFKGEPIGLSHLITKPSHSIINQAFDCRLRLDARPVNADGFGVGWYDTELEDSVPCVFQAITPAWSNRNLHRLAEKIRSPLVFAHVRASTTGALSEENCHPWIYGSLLWMHNGNISGFTKIKRHLQNELSEEFFHFPQGSTDSEWAFALFLNELSKIADPKATSFPYVTLKEVMLLTIEKIKCWWVQAGVQEPCRMNFAVTDGRSVVATKYVTSSTEDAASLFFSTGTSFEPSKQVNIPPPTYLDDDHQPRPAEQYRMQKYDKREKIVLIASEPLTFEKTDWIEIPCQTLVVVTPKMNVLQFPIIDEFVDNVYKTGISLTSKSFYHVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3LA21</accession>
    <name>E3LA21_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19414</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178391">
        <property type="protein sequence ID" value="EFP93396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337815.1">
        <property type="nucleotide sequence ID" value="XM_003337767.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LA21"/>
    <dbReference type="GeneID" id="10537263"/>
    <dbReference type="KEGG" id="pgr:PGTG_19414"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19414"/>
    <dbReference type="HOGENOM" id="CLU_1082334_0_0_1"/>
    <dbReference type="InParanoid" id="E3LA21"/>
    <dbReference type="OrthoDB" id="2507331at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="147"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="158"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="188"/>
            <end position="199"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="257" mass="28950" checksum="E32F6F4C181D2387" modified="2011-01-11" version="1">MIDNFVPVIKFRQDKENQKNGNDARECRRCKKRKASREFHRDWWTNDQGRETYRRTCQDCRGLPLRLPAVGQPTAAELAPPGEKAISGLLGTLSRQVFLDLTNDPSRLIRERIFDHLRLSSLVDSDHSSPSSLGSHCLSRADAWGTENRELGGPQTAAKEDRQRTEDLEQRSENQKEESLGSEPPTRPYNTSECVSDISSPGCDARSTGTRNLPKLERPIHNQLWPATPTFSFRMVVGADQRSNDGLDDPTCPNGLH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3LAE2</accession>
    <name>E3LAE2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18931</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178396">
        <property type="protein sequence ID" value="EFP93517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337936.1">
        <property type="nucleotide sequence ID" value="XM_003337888.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LAE2"/>
    <dbReference type="STRING" id="418459.E3LAE2"/>
    <dbReference type="GeneID" id="10543632"/>
    <dbReference type="KEGG" id="pgr:PGTG_18931"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18931"/>
    <dbReference type="HOGENOM" id="CLU_065209_0_0_1"/>
    <dbReference type="InParanoid" id="E3LAE2"/>
    <dbReference type="OMA" id="EIFMHER"/>
    <dbReference type="OrthoDB" id="2507659at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="408" mass="46508" checksum="80C87049E3FDCB69" modified="2011-01-11" version="1">MVAQKEGLEEEHVNTGETEVNHTKEPRIVKEELRVLQDVITQTEIPTWASRVPHNFGLSGSGSLKAADWLILYTVYYPLAMVPYWVIGNDWQKQKIDGSTSVTHQDVLRDSLVQLIEITNILMMHHLEPNDITKYSKLIWEYRKTLKLGWPTQSSKPNLHLSQHYPKVIKSLGPPMATSAWAQERLNGMLGKIPRNNHIGELNKTLMSSWNKKALYYGMINQRNLGTEGSTTDSHVKKTMTTNQTNAILDSRVYKQWKVQLENMGFRGNAVEGEIKNVVITVKSVQIFNRRYVAAKDLFEKPPGNSSVEIDRGGDRIFGRISLLFMGQSREDVWFVVRPFASLNRWDEAKNPYKGLPQLNVKLIYSRKLEKAVVVHQSQVVGHIATLRNKEGTFGINQETISAIQVAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3LAI4</accession>
    <name>E3LAI4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19513</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178398">
        <property type="protein sequence ID" value="EFP93559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337978.1">
        <property type="nucleotide sequence ID" value="XM_003337930.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LAI4"/>
    <dbReference type="GeneID" id="10534767"/>
    <dbReference type="KEGG" id="pgr:PGTG_19513"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19513"/>
    <dbReference type="HOGENOM" id="CLU_2513737_0_0_1"/>
    <dbReference type="InParanoid" id="E3LAI4"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="15"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="85" mass="9991" checksum="8954C8ECDFB67E67" modified="2011-01-11" version="1">MDLSWRLDLRWRLYYYYYFLLGGDGLFGMWAAEPIFVLKRRRGSRAEEMKKILGLAGWLAGWVEGCVQIVEADELVSVVFRRLLA</sequence>
</entry>
<entry dataset="Swiss-Prot" created="2012-03-21" modified="2025-06-18" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>E3LAQ9</accession>
    <name>NNRD1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-dependent (S)-NAD(P)H-hydrate dehydratase 1</fullName>
            <ecNumber evidence="1">4.2.1.93</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">ATP-dependent NAD(P)HX dehydratase 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name type="ORF">PGTG_19583</name>
    </gene>
    <organism>
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain>CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <reference key="2">
        <citation type="journal article" date="2017" name="G3 (Bethesda)" volume="7" first="361" last="376">
            <title>Comparative analysis highlights variable genome content of wheat rusts and divergence of the mating loci.</title>
            <authorList>
                <person name="Cuomo C.A."/>
                <person name="Bakkeren G."/>
                <person name="Khalil H.B."/>
                <person name="Panwar V."/>
                <person name="Joly D."/>
                <person name="Linning R."/>
                <person name="Sakthikumar S."/>
                <person name="Song X."/>
                <person name="Adiconis X."/>
                <person name="Fan L."/>
                <person name="Goldberg J.M."/>
                <person name="Levin J.Z."/>
                <person name="Young S."/>
                <person name="Zeng Q."/>
                <person name="Anikster Y."/>
                <person name="Bruce M."/>
                <person name="Wang M."/>
                <person name="Yin C."/>
                <person name="McCallum B."/>
                <person name="Szabo L.J."/>
                <person name="Hulbert S."/>
                <person name="Chen X."/>
                <person name="Fellers J.P."/>
            </authorList>
            <dbReference type="PubMed" id="27913634"/>
            <dbReference type="DOI" id="10.1534/g3.116.032797"/>
        </citation>
        <scope>GENOME REANNOTATION</scope>
        <source>
            <strain>CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(6S)-NADHX + ATP = ADP + phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19017"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction>
            <text>(6S)-NADPHX + ATP = ADP + phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32231"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.93"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.93" evidence="1"/>
    <dbReference type="EMBL" id="DS178401">
        <property type="protein sequence ID" value="EFP93634.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338053.2">
        <property type="nucleotide sequence ID" value="XM_003338005.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LAQ9"/>
    <dbReference type="SMR" id="E3LAQ9"/>
    <dbReference type="FunCoup" id="E3LAQ9">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3LAQ9"/>
    <dbReference type="EnsemblFungi" id="EFP93634">
        <property type="protein sequence ID" value="EFP93634"/>
        <property type="gene ID" value="PGTG_19583"/>
    </dbReference>
    <dbReference type="GeneID" id="10534923"/>
    <dbReference type="KEGG" id="pgr:PGTG_19583"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19583"/>
    <dbReference type="InParanoid" id="E3LAQ9"/>
    <dbReference type="OrthoDB" id="8110916at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047453">
        <property type="term" value="F:ATP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110051">
        <property type="term" value="P:metabolite repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1190.20:FF:000023">
        <property type="entry name" value="ATP-dependent (S)-NAD(P)H-hydrate dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592:SF0">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword id="KW-0067">ATP-binding</keyword>
    <keyword id="KW-0963">Cytoplasm</keyword>
    <keyword id="KW-0456">Lyase</keyword>
    <keyword id="KW-0520">NAD</keyword>
    <keyword id="KW-0521">NADP</keyword>
    <keyword id="KW-0547">Nucleotide-binding</keyword>
    <keyword id="KW-0597">Phosphoprotein</keyword>
    <keyword id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_0000416189" description="ATP-dependent (S)-NAD(P)H-hydrate dehydratase 1">
        <location>
            <begin position="1"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="domain" description="YjeF C-terminal" evidence="1">
        <location>
            <begin position="80"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="240"/>
            <end position="246"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="280"/>
            <end position="284"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="300"/>
            <end position="309"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="310"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000255" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03157"/>
        </source>
    </evidence>
    <sequence length="398" mass="43998" checksum="E74B5EAA9E81AB17" modified="2012-10-03" version="2">MVLTTRHHLDGFRSIQVMLGALEHPLDVMKASKGRSCSENDHRMYRFSKNLPRGVCSPLTTGPHPTTHLEPTMHQPHRSLLRKAFQMIPPLDGSLHKGQAGRIGIVGGSKDYTGAPFYSGYASLRLGSDLSHVICEPSASTVIKTYSPDLMVHSYLSSPKEPEAYASHQNLFEQLLDRLHVLVVGPGLGRDTEMQDWAEWTLKTAIKKKLHLVLDADALWLLVKKPDLLRGYPNAILTPNHVEFQRLLKACSIEPRENDDDGLLAMELSKALGGCSILQKGSIDLVAREGSEVAKVSCEGSPKRCGGQGDILSGLVGTWCAWTKLYFERQSQDEKPKSHELPISSEEAWIIAAVLGSEITRTCSRLAYHKLGRSMQSSDMLGYIGEAFELVMHGHTKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3LBW5</accession>
    <name>E3LBW5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_20034</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the replication factor A protein 3 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178430">
        <property type="protein sequence ID" value="EFP94040.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338459.2">
        <property type="nucleotide sequence ID" value="XM_003338411.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LBW5"/>
    <dbReference type="STRING" id="418459.E3LBW5"/>
    <dbReference type="GeneID" id="10535730"/>
    <dbReference type="KEGG" id="pgr:PGTG_20034"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20034"/>
    <dbReference type="HOGENOM" id="CLU_725906_0_0_1"/>
    <dbReference type="InParanoid" id="E3LBW5"/>
    <dbReference type="OrthoDB" id="188186at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005662">
        <property type="term" value="C:DNA replication factor A complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035861">
        <property type="term" value="C:site of double-strand break"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04479">
        <property type="entry name" value="RPA3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013970">
        <property type="entry name" value="Rfa2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15114:SF1">
        <property type="entry name" value="REPLICATION PROTEIN A 14 KDA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15114">
        <property type="entry name" value="REPLICATION PROTEIN A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08661">
        <property type="entry name" value="Rep_fac-A_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="29"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="46"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP94040.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="381" mass="41670" checksum="F1E8609F3CB3F7AF" modified="2012-04-18" version="2">MSLPYSTPDLEDGFAPSTPFRNQIFDRPSGSSSAFNWNPDTTLPPPKDKGKGKASEMRDTTEEDSDAGEPTPQAHNLPPLSGYGLHNTQNAEEPVYNRQQQQQPANQEQADQQPIPQLDNLHFVDAILYSDPRVLDTVQRIGNNTKADKKVAEGGHVACRMSHRLAIGAADADDNKITLILKDDVDIEAAFKYLAGTILRDVPKDWDIIFFGHTDFSNKARNGSDPSTHNFYIYKSVKPQAANPIKQTSHSVILVMTPSSSPAVILNIETKMDVPTPRVSSSLLGQYVGQVVRISGKVISLSPELLIESSDGGQVTVGDSAACGTEITDEFIELVAKVETETRVKTLDCSNLGDKYDLKLAQAVVDIIHNPNLRCERAPCF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPY5</accession>
    <name>H6QPY5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CNH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20971</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EHS64508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890422.1">
        <property type="nucleotide sequence ID" value="XM_003890373.1"/>
    </dbReference>
    <dbReference type="STRING" id="418459.H6QPY5"/>
    <dbReference type="GeneID" id="13542639"/>
    <dbReference type="KEGG" id="pgr:PGTG_20971"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20971"/>
    <dbReference type="eggNOG" id="ENOG502S22M">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002840_0_0_1"/>
    <dbReference type="InParanoid" id="H6QPY5"/>
    <dbReference type="OrthoDB" id="6415790at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="8"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="232"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="377"/>
            <end position="399"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="409"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="504"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="563"/>
            <end position="709"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1276"/>
            <end position="1326"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1373"/>
            <end position="1431"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="39"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="240"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="583"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="637"/>
            <end position="662"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="676"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1278"/>
            <end position="1312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1373"/>
            <end position="1420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1421"/>
            <end position="1431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1431" mass="150101" checksum="310A3975A55E86B3" modified="2012-04-18" version="1">MIGKRNFFSTLLQRASGSNSNPTSPGPSSAPGSASRNPFESTFNRPTHSDGTLRGPHNRLGLESHGTSSQPMDAANSDKTASPGMNSSYATALTLGSMNLKISPLTPTLPASRNSQPLCGAILDDKFLLIGTNSGLDFVPLTPFTKSSNSSDRSRGSVHTVKPISLIKKTRFKSLKVLEVRSNILLAIAGRNDHLRVYALDGIRAIVAKKIQDLEEKEDFVWLPKTLARVTPHKGKERVANAPAQPNASNSPQYSPPPPYGGKLGPGQTMAKSSSCQPTTVANQPSQATKSPVKTALRRSSTVGSRLPSGTSAFEVADTRRSSLHSTSDSSSFVSPLGLLGPTLTSALQSLRAENSDQLPTNHMALGRDAVQVGLEQATEERENGSSQRGPPPIINTDDVLARRPNMTGIHEGERTGKPEPNNGQGEPAGTALVEVIKKRSQLLAQAGKRIPNPNLPIQPITSSRLVPRSALATDTDNESVDGSQVNLVDVLSQPAANSAALGISASAPAREPRTSMGEVPSVLKKPTANTQSVPGPPLNAPNDVHPQINVTDEIAELGHHPIVGSTSLPRAGLNPMADDASRANSATPSALLSSRTPSRVPSGVNSDQENQPSSLTVPSRSKKRWSVMDSVFRTHNGSSSSSGSSNNNSHSNSPNPAGPSGLLPARSSADDSLVRSASCNQFSPSPFSEAANGQQNPETAKTSPHPATLGAPLEYVKLARTKGAKLLRAYETRRRTYLAVLCGESSERIELFTGSKNISLSLNRTFVLPETPRTIEFQMQGDDLVDIYLVYDESVFALEPATVRVREVGIGRNERRAARRERERAARDLASINMRPAVTATGSNSSAVAHQSRRFMNDPTESLMEAVGAPARSTISASPSTVDDGLTLSAPVPSSSFRQASAANTTSIASGWGSHDSPVASGGNHPGAAKVPNLWPYTTFQQLQFIPPLPPSVLASTFVIPPTYEAVVTASGGPRAVQNSLVPPDEDPARTLSPINVVEPESENLTIPLNLAVDQLSINNGSSSVLGEAGSEAGSSIANSGGSVGASKINASDGSEAEMEYSDSGDATVTVESGRQVPVASPNPQTILADGPLLSPISLLSNPASRQIGPPGLFLVTRGKKVTSIVDCDGRSVMKKPFIWMNDKHSKPSGSAAENGQHPTGFRIEVVLIDERRTVLVGLDHQEVKLFEVGGRQAKGLEEFSNSATLDILPHLILGGSPNGPTSLPTGVGGGNGVGVITAQSFPSSHSFRDHHPHHHSTLNLSSLTNLTIGLSKWSDSSSHSNHNNHNSGGSSFAGSLAAPNQPSNPSSSSPGTGGGSGLSGNSSSILIPPVDREVVFLASSVSRNSVIWSERIGNSFAIYSLSSSLPFLSHSHPTTTSTTTNPVTTTTTNSNINTNTSSSSTSTTTTNPNLNHSNLPPTQLNHSLPTSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQR8</accession>
    <name>H6QQR8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21137</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EHS62816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890135.1">
        <property type="nucleotide sequence ID" value="XM_003890086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQR8"/>
    <dbReference type="GeneID" id="13541468"/>
    <dbReference type="KEGG" id="pgr:PGTG_21137"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21137"/>
    <dbReference type="HOGENOM" id="CLU_033090_2_1_1"/>
    <dbReference type="InParanoid" id="H6QQR8"/>
    <dbReference type="OrthoDB" id="2513067at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS62816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="394" mass="45723" checksum="8FCBBAD289DDF532" modified="2012-04-18" version="1">MTNCEMSRSTQADPVLEALQVLVDTHHDRSRSPHPSNTWEENGELAKDDLDEKKKLLDELRSSLLPLMKHELKTLSTALDRPHDSSEYTSPDIKLTLQTLSNLDLTMQKSLHYVKSSPLKALQPTDTCDHHFEQCKSFRLFNLETQIGFLEMHLSFTFSAYLKSFRSSNLPIKRPDDPGYRAPTPREETNSCLYEIDQAIKWSQASDFALLQLKWRGVVKGFHDLLARLASLIGRRQENNNVFRNYIVEQARLNIPIIKLLRTLYHKISNTTTKKLMFTLDTEVNSNTLHTLNQEPDIILYNCTVHVKCLDESYRYNSMTDTSRSLYDRNKCLSQTVESTVALLALYIIPLSPKVDHSSLESDFKAWLSEWHVLWQTAKQRFMDAMYIYEEENQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRA3</accession>
    <name>H6QRA3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Reverse transcriptase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21424</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EHS63094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889955.1">
        <property type="nucleotide sequence ID" value="XM_003889906.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRA3"/>
    <dbReference type="GeneID" id="13540539"/>
    <dbReference type="KEGG" id="pgr:PGTG_21424"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21424"/>
    <dbReference type="HOGENOM" id="CLU_003292_3_3_1"/>
    <dbReference type="InParanoid" id="H6QRA3"/>
    <dbReference type="OrthoDB" id="2678913at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052055">
        <property type="entry name" value="Hepadnavirus_pol/RT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050">
        <property type="entry name" value="REVERSE TRANSCRIPTASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33050:SF7">
        <property type="entry name" value="RIBONUCLEASE H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="266"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="273"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="286"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="315"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="329"/>
            <end position="344"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="736" mass="83911" checksum="503DEF80E5E2495A" modified="2012-04-18" version="1">MINDKDDIQIISESNKAERLRDEATAKFLEKALKAEGEGDKARSDIGPVATKDTESISMFGATTDHPVKGSRKNTTVGGTNFHWGDKNSHEDVGFTPYFEKNITKLKGPIPLTIFNKAWQDAALSYHAEKRTKTDDNSTEKGLRYTGYPYPSEWLMNYSDWSLNYEEFTITIRDVYKFETLGEWIVLHKANCDKILRKDGFMTALRYDIRIRANAFAHRVVRNGVSSFSDISMFRQEVYDTAYAEARRFDELSFCDSNPYAIGGVRSGWDPHTGSNQNKKASGQQSSVKPTPTGPSGSTNTLPPKPRDNRPARSSGYQGKNFNPNYAEQRQGGNQNRNSNNNNGPETDPSEQPYMAVSEVSECQKELPVEGTWPSGIECDMNIPEWKKALEKANLTTEFEDVLLGFEHGFCQGIPQHHLGSEFPFFVPPNHSSALLARAKIEESIAKEIQAKRMFGPYSPKQVHAKYGFFRANPLGAVINGDGSFRAINDLSFPHGDKKIPSVNSFVNKDEFKTTWDDYKAVARFLRARERPSLLAIFDWEKAYRQILTAENQWPYLMLRDFDGNIIIDTKIAFGGVAGCGSFGRPADAWKHIMLSEFDLVEVFRWVDDYLFVKELESTVEMDKIVKRSDELGVRMNPTKISPFKREQKYIGFIWDAHDKTVHLPEEKLAQRITQIGEFLETEKKFSFNEVEALTGQLNHVSYMLPQLRCYLCGLYRWMCSWVHRNTKLPICKVWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRZ2</accession>
    <name>H6QRZ2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21560</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EHS63427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889712.1">
        <property type="nucleotide sequence ID" value="XM_003889663.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRZ2"/>
    <dbReference type="STRING" id="418459.H6QRZ2"/>
    <dbReference type="GeneID" id="13542809"/>
    <dbReference type="KEGG" id="pgr:PGTG_21560"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21560"/>
    <dbReference type="HOGENOM" id="CLU_023058_0_0_1"/>
    <dbReference type="InParanoid" id="H6QRZ2"/>
    <dbReference type="OrthoDB" id="2507825at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="528"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="572"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="620"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="658"/>
            <end position="667"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="689"/>
            <end position="734"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="734" mass="78723" checksum="906A870A826F0EB8" modified="2012-04-18" version="1">MGCMGCIELSGDTAPGKGFGPFLCRTDTASNCLHDCSTHDTVVMNQGYPPDNPPPGQGSQHPQPHHDPDSFRPLYNRVIGPHPSLAADWSQSTGIHPSEQPAARTIQVANSLTYGQTVRIHPAGAPAVVNHPNYPQADHYTNHNPVHPAPGTVGSVQVVLLSDAYKGRPSLAHHTTTTQDYPAGSVTRLVSLGPALQGQPVIHPPATQLPPAVIPTPTPTGSSVVTTPVVNGGIRTCKPKRTAEEMRLADLVAAEKRARRFQNQADKAAEARQKQATNAANRMLKAQKAASATPRATWSEEQTIELLNYVRMVKDDHSQVTGGFIPFGKYFVAYTGHEEAFPLLDSVLPATRLAKYRAVMDKWKSKMSSTGVDKRTPTPMVHANLHARRRLACRPTRQRSLATHAWVLWDGGTPKRICLWRAGLHTGLHSVDWRASPHANLLWRAGAHANPHLACGLACAMGAGVCLSMPVVDRSGAGGLSSALDAGNLSQEIWDLILDMHGDNPAATAEGLTGSSADYETLLQEEVNSGASSGPGSSDIEAPDSLPATPATRNKRQTKYQRLCAGLTPAELALDPDSSSDCDLPDELPLGAPGTTRTGVNVTPIPGVPPLTPVLGTPASIPTGPPMGSKRKARALSSKQPVSRPPAEVPTIPRRRGRTEEKTKEGDTTGTGMLVMMHKAQESSASWAAEERRDAQVERARQDVIRAEERADRQRIEDRQAAERAAEIKRQDDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUL9</accession>
    <name>H6QUL9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_22460</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178346">
        <property type="protein sequence ID" value="EHS64735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888799.1">
        <property type="nucleotide sequence ID" value="XM_003888750.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUL9"/>
    <dbReference type="STRING" id="418459.H6QUL9"/>
    <dbReference type="GeneID" id="13541609"/>
    <dbReference type="KEGG" id="pgr:PGTG_22460"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22460"/>
    <dbReference type="HOGENOM" id="CLU_1678794_0_0_1"/>
    <dbReference type="InParanoid" id="H6QUL9"/>
    <dbReference type="OrthoDB" id="2506362at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="98"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="137"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS64735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="157" mass="18146" checksum="D19D442959F6D82A" modified="2012-04-18" version="1">MKEKHYNAKKIKVLTSRSNDYRDRTLAMKRTNEIRSEAARAEVNQVNMDIMSRRKEDLPDAISKEFLQIQKEMIVYDMREQLEERRKSAAQKAKETKQSTSSSTQQATTSQQQLLSSSLTPTSEPNDESTPNSSSEEYVDEEQAYPEEIDPTLYSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3JPT2</accession>
    <name>E3JPT2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Rab-GAP TBC domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_00228</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74272.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307278.2">
        <property type="nucleotide sequence ID" value="XM_003307230.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JPT2"/>
    <dbReference type="FunCoup" id="E3JPT2">
        <property type="interactions" value="647"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JPT2"/>
    <dbReference type="GeneID" id="10528031"/>
    <dbReference type="KEGG" id="pgr:PGTG_00228"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00228"/>
    <dbReference type="eggNOG" id="KOG1092">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018687_6_1_1"/>
    <dbReference type="InParanoid" id="E3JPT2"/>
    <dbReference type="OrthoDB" id="26371at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005096">
        <property type="term" value="F:GTPase activator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.270:FF:000037">
        <property type="entry name" value="TBC1 domain family member 22A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.80:FF:000001">
        <property type="entry name" value="TBC1 domain family member 22B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.270">
        <property type="entry name" value="putative rabgap domain of human tbc1 domain family member 14 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.750">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.80">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000195">
        <property type="entry name" value="Rab-GAP-TBC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035969">
        <property type="entry name" value="Rab-GAP_TBC_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957:SF26">
        <property type="entry name" value="LD44506P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22957">
        <property type="entry name" value="TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00566">
        <property type="entry name" value="RabGAP-TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00164">
        <property type="entry name" value="TBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47923">
        <property type="entry name" value="Ypt/Rab-GAP domain of gyp1p"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50086">
        <property type="entry name" value="TBC_RABGAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rab-GAP TBC" evidence="2">
        <location>
            <begin position="233"/>
            <end position="458"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="134"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="78"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="96"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP74272.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="530" mass="60164" checksum="D1BD46BA09D80F1F" modified="2012-04-18" version="2">MITTTPPGSSSRSTTHNSKKKNQLEEKQRERELIEDTDQAWDDHSDQEESKTTTTQPIIRTRKLSLENNPSHQAHQSTPPPPPPPPTIQSNPLDQSDTIGSPSASTSSTHSTFTKDIFSLIKDPAYILSRLKTEYEQQEQSSPLDTPSRRGRGSLPWEAVIPAELMTKKAAGSRSPSPTPPTGSGPGPYTLLPETPNQPGPSSEALKREQKFVDCLESPTVDVGGLRKLAWAGIPPRLRALVWMILLGYLPAPQSRRLEVLARKRREYLDALRLAFASGTQKLDQTIWHQIQIDVARTNPGVPLWQFHATQRSLERILYVWAIRHPASGYVQGINDLVTPFFQVFLTAYIDGEEAESFDVGRLPAEALEAIEADSFWSLSKLLEGIQDNYIFAQPGIQRQVARMKELCERVDARLHRHLEDEKVEFIQFAFRWINCLLMRELSTKKIIRMWDTYLAEGTAAFSEFHLYVCLAFLVRYSDQLREMDFQSIIIFLQALPTDQLTEKDLEFLLSQAFMWHSLFQGATGHFVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQA7</accession>
    <name>E3JQA7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Elongation factor EF-1 beta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_00332</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
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    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    <keyword evidence="3 4" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Elongation factor 1 beta central acidic region eukaryote" evidence="8">
        <location>
            <begin position="102"/>
            <end position="129"/>
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    <feature type="domain" description="Translation elongation factor EF1B beta/delta subunit guanine nucleotide exchange" evidence="7">
        <location>
            <begin position="138"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="69"/>
            <end position="100"/>
        </location>
    </feature>
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        <location>
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            <end position="219"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="81"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM00888"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SMART" id="SM01182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP74376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="224" mass="24717" checksum="D55A390AC50491ED" modified="2011-01-11" version="1">MGFDFTGNDYSQLEQHVYDKSYIEGYAPSQADVVVFQAIASSPDVKKYPQAARWWAHIKSWESEHASLPGEKKELSSYGPAKGSTSAPAAAAGGDDDDEIDLFGSDDEEVDEAAEKLKATRLAEYAAKKANKPKTIAKSLVTLDVKPWDDETDMAELEKAVRSVEMDGLVWGLSKLVPVGYGVRKMQISLVVEDEKVSLDDLQEKIAEFEDYIQSSDVQAMQKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3JR23</accession>
    <name>E3JR23_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_00287</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
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    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP74331.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="192" mass="21086" checksum="B180463D08036C81" modified="2012-04-18" version="2">MSGKLSTSVDSNVDANDTSSLKVSGIVQLKSQGSESNYLDWSFVVLLHLQSLNLAYVLNTVAVEDRTAAYLRDSVAVSSFIARTIHPSNLRFIRAHGSDAAAMWSSLSEAHQDFSSGGRMHWLRKLVVSRLVGDDIEAHIESMAVCAERLNSLVTVDKPLTPDDIYSTALLTSLTDDWLPSLPLPQNEYQSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>E3JR96</accession>
    <name>E3JR96_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Histone H3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_00393</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
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    </reference>
    <comment type="subunit">
        <text evidence="8">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Chromosome</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the histone H3 family.</text>
    </comment>
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        <property type="protein sequence ID" value="EFP74437.1"/>
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    <dbReference type="KEGG" id="pgr:PGTG_00393"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00393"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="evidence" value="ECO:0000318"/>
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        <property type="term" value="F:DNA binding"/>
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        <property type="term" value="F:protein heterodimerization activity"/>
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        <property type="project" value="InterPro"/>
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        <property type="project" value="InterPro"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Histone H3"/>
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    </dbReference>
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        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
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        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11426">
        <property type="entry name" value="HISTONE H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00322">
        <property type="entry name" value="HISTONE_H3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00959">
        <property type="entry name" value="HISTONE_H3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="3" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0488">Methylation</keyword>
    <keyword evidence="7 8" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="10">
        <location>
            <begin position="44"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="47"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP74437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="136" mass="15355" checksum="403916D07E60DF8E" modified="2011-01-11" version="1">MARTKQTARKSTGGKAPRKQLAAKAARKSAPSTGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQESAEAYLVGLFEDTNLAAIHAKRVTIQPKDIQLARRLRGERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3JSI0</accession>
    <name>E3JSI0_PUCGT</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_01546</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP74953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003319324.2"/>
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    <dbReference type="KEGG" id="pgr:PGTG_01546"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01546"/>
    <dbReference type="HOGENOM" id="CLU_1086387_0_0_1"/>
    <dbReference type="InParanoid" id="E3JSI0"/>
    <dbReference type="OrthoDB" id="2498143at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP74953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="256" mass="29332" checksum="3040EDEBC1AD3B55" modified="2011-01-11" version="1">MDNPLDEVGPQIKHLGDLVIQGFGKLKDKYQTHRSTNQVARTTHAVINDQANSREIHYDRLHSSLLPQLKEKLVTISRLLEPQDIWEEPEARLRRLLEIIPELDSTLSQIHFSTLIVCPGTLFSASQTNDQDLKEFKTFRLIKLKGTIDHMLWNLCSVCNSASIHIEIIELSTGGLFMHVNESSFYGSYRQALRWIRFTISQLKGSELDVVEECRKIPILSIDKLLQDTLSILTPSAPKTRYFQRKLTQQATVKPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3JSI4</accession>
    <name>E3JSI4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="7">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_01550</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="7"/>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP74957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319376.1">
        <property type="nucleotide sequence ID" value="XM_003319328.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JSI4"/>
    <dbReference type="STRING" id="418459.E3JSI4"/>
    <dbReference type="GeneID" id="10547239"/>
    <dbReference type="KEGG" id="pgr:PGTG_01550"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01550"/>
    <dbReference type="HOGENOM" id="CLU_011478_0_2_1"/>
    <dbReference type="InParanoid" id="E3JSI4"/>
    <dbReference type="OMA" id="IRIEMAY"/>
    <dbReference type="OrthoDB" id="5409596at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="41"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="460"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="465"/>
            <end position="479"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP74957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="492" mass="55239" checksum="195F820ACCC1E81A" modified="2011-01-11" version="1">MGASLYKKIYEKSDDKLKEFIIESSIKTYQQEPKRLQVDAVFNLARGRNTFLLAGTGFGKSRISEMYFNMIPKMKRAVVLVLNPLDSLGDNQVLEKEKAGFTAINLTKMNFNKKTADAILNGDYNFIYLSPEIFLNSKSFDQVYFSTTFQNRLALIVIDEAHMVYIWGLVESSTSKIVTSVHLRIEDYALFRPCYGKLGPHLLFRNDKPILLLSATCRPVAVEGIMKSLKLTDDNLDILRGELTRPEIRIIRVEMDYSLASSLDLVKVFPSANDVPDEEMVPSLIYSSSRNRTLIAMDVVDLARETPGAAFRSPSSCIRRFHSCTGDQDKVDCIDDFASGKFPLISCTMALGLGQNWKRVRMVTHLGRGDPASISQMFGRCGRDGRQGLAIMFVEKIRRNGKNSVDQFGQGTYQSDLDRLDALAITPVCLRVAFAIDNLLGYIPLSVDDPGYIQEKAREIKEGMTESNSDSQYQPQISLQACGHEEEEDSRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3JT69</accession>
    <name>E3JT69_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Holocytochrome c-type synthase</fullName>
            <ecNumber evidence="10">4.4.1.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_01941</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Lyase that catalyzes the covalent linking of the heme group to the cytochrome C apoprotein to produce the mature functional cytochrome.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>holo-[cytochrome c] = apo-[cytochrome c] + heme b</text>
            <dbReference type="Rhea" id="RHEA:22648"/>
            <dbReference type="Rhea" id="RHEA-COMP:10725"/>
            <dbReference type="Rhea" id="RHEA-COMP:10726"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:60344"/>
            <dbReference type="ChEBI" id="CHEBI:83739"/>
            <dbReference type="EC" id="4.4.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the cytochrome c-type heme lyase family.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.17" evidence="10"/>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75348.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319767.2">
        <property type="nucleotide sequence ID" value="XM_003319719.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JT69"/>
    <dbReference type="STRING" id="418459.E3JT69"/>
    <dbReference type="GeneID" id="10542895"/>
    <dbReference type="KEGG" id="pgr:PGTG_01941"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01941"/>
    <dbReference type="eggNOG" id="KOG3996">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_048602_0_1_1"/>
    <dbReference type="InParanoid" id="E3JT69"/>
    <dbReference type="OrthoDB" id="4243at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004408">
        <property type="term" value="F:holocytochrome-c synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000511">
        <property type="entry name" value="Holocyt_c/c1_synthase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12743">
        <property type="entry name" value="CYTOCHROME C1 HEME LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12743:SF3">
        <property type="entry name" value="HOLOCYTOCHROME-C SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01265">
        <property type="entry name" value="Cyto_heme_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00821">
        <property type="entry name" value="CYTO_HEME_LYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00822">
        <property type="entry name" value="CYTO_HEME_LYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-0349">Heme</keyword>
    <keyword evidence="6 10" id="KW-0408">Iron</keyword>
    <keyword evidence="9 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="5 10" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="48"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="86"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP75348.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="308" mass="35452" checksum="CB5BF36716AF24AB" modified="2012-04-18" version="2">MGSSQSTPQQDTNKLSQCPIQHQNNNPLQSSTNFNESNNIPELPQAPSPGQRTKLSLQRTLSSIPRPSSPAFSAEEEDNTQVKALGNSSCPVHQSTPKKEKDTWVYPSPQQFYNALVRKGWETPEESIPIMVDIHNWMNEAVWNEVLRWESRYFFTPSSSSSSNNHPVQQQEEQQPEISLVKFQGRPHELSPKARFNILLSMIFPNLYNPVRPFDRHDWSICREHLLKQDPQVEKSKLCRYVIDYYNGGIDEDGNPVFHFDVRPAIDDLDSLVVRVKEWARIKNETWFKTPDQSTKTHPNIVQGDSKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3JTX0</accession>
    <name>E3JTX0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_00810</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319898.1">
        <property type="nucleotide sequence ID" value="XM_003319850.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JTX0"/>
    <dbReference type="GeneID" id="10543109"/>
    <dbReference type="KEGG" id="pgr:PGTG_00810"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00810"/>
    <dbReference type="HOGENOM" id="CLU_1826251_0_0_1"/>
    <dbReference type="InParanoid" id="E3JTX0"/>
    <dbReference type="OrthoDB" id="10584147at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP75479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="141" mass="15253" checksum="BBE508BCE8609C6D" modified="2011-01-11" version="1">MVHSQASTFNPIALGLENPDIRSDRTVSISRKGVTRKTPPGCFDGWCHPGVGPRGGSLDPPEHPGGSAIWVDGRYELVFWLGLNVTKSFSMYLSGGMICSRLGHDVVPIKVCTWEEERVKPPFNFDIFTGVGAGVVAIRVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUP0</accession>
    <name>E3JUP0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_01096</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320184.1">
        <property type="nucleotide sequence ID" value="XM_003320136.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JUP0"/>
    <dbReference type="FunCoup" id="E3JUP0">
        <property type="interactions" value="359"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JUP0"/>
    <dbReference type="GeneID" id="10543757"/>
    <dbReference type="KEGG" id="pgr:PGTG_01096"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01096"/>
    <dbReference type="eggNOG" id="KOG1165">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_019279_1_2_1"/>
    <dbReference type="InParanoid" id="E3JUP0"/>
    <dbReference type="OMA" id="VFRATQT"/>
    <dbReference type="OrthoDB" id="5800476at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000324">
        <property type="term" value="C:fungal-type vacuole"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14127">
        <property type="entry name" value="STKc_CK1_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000160">
        <property type="entry name" value="Casein kinase I 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000538">
        <property type="entry name" value="Putative Casein kinase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="12">
        <location>
            <begin position="19"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="315"/>
            <end position="509"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="339"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="363"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="380"/>
            <end position="398"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="411"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="11">
        <location>
            <begin position="472"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP75765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="522" mass="58120" checksum="A4F45EA3AA5E87B8" modified="2011-01-11" version="1">MSAAHSSHNSSSNVVGVHFRVGKKIGEGSFGVIFEGTNLLNSQTVAIKFEPRKSDAPQLRDEYRTYKILAGSPGVPQVYYFGQEGLHNILVIDLLGPSLEDLFDMCGRKFSVKTVVMTAKQMLTRVQTIHEKNLIYRDIKPDNFLIGRPGTKAANVVHVVDFGMAKLYRDVKTKVHIPYRERKSLSGTARYMSINTHLGREQSRRDDLEALGHVFMYFLRGGLPWQGLKAATNKQKYEKIGEKKQSTAIKELCENFPEEFGIYLNYVRKLGFEETPDYDFLRELFTKVLKNTGETEDGVYDWMILNNGKGWEASSTNHAHLHPGGAAHQYDNRHHRSSRDRERERTERHAMKASQAAGLQYPNDLTRQGSKPERTSTNLSMGKQMPGGMPQGTGTSSTAALQGVPPPDNGILQSSSGKRQSHQSANRQEANPSRSSNHPYASPNPASGTGQTFDGSNNQRASYGNMPAHLNNHLQANSQPNHQSSPDPLTSGQKRPIKSNDNQDPPRSIGLKILDFLTCRCG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3JV72</accession>
    <name>E3JV72_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01278</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75947.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320366.2">
        <property type="nucleotide sequence ID" value="XM_003320318.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JV72"/>
    <dbReference type="GeneID" id="10533025"/>
    <dbReference type="KEGG" id="pgr:PGTG_01278"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01278"/>
    <dbReference type="HOGENOM" id="CLU_156039_0_0_1"/>
    <dbReference type="InParanoid" id="E3JV72"/>
    <dbReference type="OrthoDB" id="2495818at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75947.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="102" mass="11076" checksum="E8364AC0B6FA727F" modified="2012-04-18" version="2">MPTMDASPHAPPVPSSLSGHAHKCSTGMRFGAFSSTPKCCLVVCLREARQIPDLTSSLKHSYRFTSKGMELMAEKGRRLLASRVHPDEPKAKRLGVSEATPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>E3JVD7</accession>
    <name>E3JVD7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Adenylate kinase isoenzyme 6 homolog</fullName>
            <shortName evidence="9">AK6</shortName>
            <ecNumber evidence="9">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Dual activity adenylate kinase/ATPase</fullName>
            <shortName evidence="9">AK/ATPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_01343</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Broad-specificity nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Has also ATPase activity. Involved in the late cytoplasmic maturation steps of the 40S ribosomal particles, specifically 18S rRNA maturation. While NMP activity is not required for ribosome maturation, ATPase activity is. Associates transiently with small ribosomal subunit protein uS11. ATP hydrolysis breaks the interaction with uS11. May temporarily remove uS11 from the ribosome to enable a conformational change of the ribosomal RNA that is needed for the final maturation step of the small ribosomal subunit. Its NMP activity may have a role in nuclear energy homeostasis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Interacts with small ribosomal subunit protein uS11. Not a structural component of 43S pre-ribosomes, but transiently interacts with them by binding to uS11.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="9">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the adenylate kinase family. AK6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="9"/>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP76012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320431.1">
        <property type="nucleotide sequence ID" value="XM_003320383.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JVD7"/>
    <dbReference type="FunCoup" id="E3JVD7">
        <property type="interactions" value="553"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JVD7"/>
    <dbReference type="GeneID" id="10533254"/>
    <dbReference type="KEGG" id="pgr:PGTG_01343"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01343"/>
    <dbReference type="eggNOG" id="KOG3347">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_079096_3_1_1"/>
    <dbReference type="InParanoid" id="E3JVD7"/>
    <dbReference type="OMA" id="QCEIFGT"/>
    <dbReference type="OrthoDB" id="10251185at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:003001">
        <property type="entry name" value="Adenylate kinase isoenzyme 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00039">
        <property type="entry name" value="Adenylate_kinase_AK6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020618">
        <property type="entry name" value="Adenyl_kinase_AK6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595:SF0">
        <property type="entry name" value="ADENYLATE KINASE ISOENZYME 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595">
        <property type="entry name" value="POS9-ACTIVATING FACTOR FAP7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13238">
        <property type="entry name" value="AAA_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 9" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 9" id="KW-0539">Nucleus</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="3 9" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="NMPbind" evidence="9">
        <location>
            <begin position="87"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="LID" evidence="9">
        <location>
            <begin position="164"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="70"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_03173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP76012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="231" mass="26421" checksum="8AA6B4C2BAD3B353" modified="2011-01-11" version="1">MAGVKSDWWAGSHREPPKAKTKNLPNLKKTTVYNQGRKGSPMATVDEDGSSRRKNPNVLITGTPGTGKTTHAEMLAQESNGALRAINIGDFVKEHGCHEGWDDEWQSWLVDDEKLLDELEPLMSSSEGGIILDWHSSEIFPERWIDLVIVLRTSHTILWDRLEKRKYSLKKIQENNEAEIMGECLEEARENYDEEIVIELDSENIDAIDSNIHRILAWIEQWKTDNQDLSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3JVI3</accession>
    <name>E3JVI3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02499</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320477.1">
        <property type="nucleotide sequence ID" value="XM_003320429.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JVI3"/>
    <dbReference type="GeneID" id="10533298"/>
    <dbReference type="KEGG" id="pgr:PGTG_02499"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02499"/>
    <dbReference type="HOGENOM" id="CLU_1278190_0_0_1"/>
    <dbReference type="InParanoid" id="E3JVI3"/>
    <dbReference type="OrthoDB" id="10628697at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="139"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="141"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="216" mass="22720" checksum="FAD6245D79B75DE0" modified="2011-01-11" version="1">MAVAQRSGPFFAAHTRSVSGVVAEFRYATLIANSDSAALRYTNRYGNSRSQRPESVTHKDTSILRPVESGRRILSPAWLENVKVALKSIKKGRPRMIDMANNPKDKSKAVDLQAVLIPRSNLTFGGALVDLGGNDAGAGSGSARLGGSSAEESAPKSAGVRSNNSASTSMSSASTLALGTVAFLVGRLAAVGGVTFQGFMPLPSWIRSIGSRKRLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3JVY0</accession>
    <name>E3JVY0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02646</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76205.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320624.2">
        <property type="nucleotide sequence ID" value="XM_003320576.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JVY0"/>
    <dbReference type="GeneID" id="10533773"/>
    <dbReference type="KEGG" id="pgr:PGTG_02646"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02646"/>
    <dbReference type="HOGENOM" id="CLU_771925_0_0_1"/>
    <dbReference type="InParanoid" id="E3JVY0"/>
    <dbReference type="OrthoDB" id="10465022at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="28"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="309"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="309"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="334"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="348"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76205.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="359" mass="35289" checksum="93371E1F3B428A47" modified="2012-04-18" version="2">MNGQGDIPSRERRGALGRVYKPADIRPLPHLTHPLITRTPDTHTHTQQPAPIPTDQSLSNTFLSRHTNFRMRSFLTAAITVALLQSASAIPLDQQQSKDGHESARSLGLDGLGLGGGAGAGGPPPGFGAGGGAGGNMMQCGGSHALIPIGLLQNNKCRMSGGGGGRGGGGGRFPQQGIVINNNNNMNGGPMINSFQPSNTNINGGGGGFGPPGGPGLCGTPGSFGGCFNPGPMPPPCAGCGGGFMPIPAPNPCIDGGCGGGFIPSPAPNPCVNGGCGGGVLPPGGFPGGFPGGLPGGFPGGSAFGMSPMVGGMSVTSSTSETEQSSSSMQMSSGMGGMPGGPGGLLLKSKDPKKSDKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXA3</accession>
    <name>E3JXA3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_02139</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321097.1">
        <property type="nucleotide sequence ID" value="XM_003321049.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXA3"/>
    <dbReference type="STRING" id="418459.E3JXA3"/>
    <dbReference type="GeneID" id="10528617"/>
    <dbReference type="KEGG" id="pgr:PGTG_02139"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02139"/>
    <dbReference type="eggNOG" id="KOG4144">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1714202_0_0_1"/>
    <dbReference type="InParanoid" id="E3JXA3"/>
    <dbReference type="OMA" id="IKYLACC"/>
    <dbReference type="OrthoDB" id="2504607at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006892">
        <property type="term" value="P:post-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007264">
        <property type="term" value="P:small GTPase-mediated signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.150.10">
        <property type="entry name" value="Metal Binding Protein, Guanine Nucleotide Exchange Factor, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007515">
        <property type="entry name" value="Mss4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011057">
        <property type="entry name" value="Mss4-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011323">
        <property type="entry name" value="Mss4/transl-control_tumour"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13276">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR MSS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13276:SF0">
        <property type="entry name" value="GUANINE NUCLEOTIDE EXCHANGE FACTOR MSS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04421">
        <property type="entry name" value="Mss4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51316">
        <property type="entry name" value="Mss4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51796">
        <property type="entry name" value="MSS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0344">Guanine-nucleotide releasing factor</keyword>
    <keyword evidence="3" id="KW-0653">Protein transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP76678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="153" mass="16695" checksum="E0F73F8698C4580B" modified="2011-01-11" version="1">MGVGFLDRGPSQVVHGSRPWSEMILHLPSKNPSKLLCPIESCRCIILRKDVGRLVTVQECPLPEPPHLTSTGPSTRCWLVPSPTSFENIGFSKPVGQVAGNERRWLSCGACDFGPLGWTESPTNLAKQFANDPQGRFGNVLLLVGCNRVFPAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXG3</accession>
    <name>E3JXG3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2'-phosphotransferase</fullName>
            <ecNumber evidence="3">2.7.1.160</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_02199</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the last step of tRNA splicing, the transfer of the splice junction 2'-phosphate from ligated tRNA to NAD to produce ADP-ribose 1''-2'' cyclic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2'-phospho-[ligated tRNA] + NAD(+) = mature tRNA + ADP-alpha-D-ribose 1'',2''-cyclic phosphate + nicotinamide</text>
            <dbReference type="Rhea" id="RHEA:23324"/>
            <dbReference type="Rhea" id="RHEA-COMP:11106"/>
            <dbReference type="Rhea" id="RHEA-COMP:11107"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:76596"/>
            <dbReference type="ChEBI" id="CHEBI:82883"/>
            <dbReference type="ChEBI" id="CHEBI:85027"/>
            <dbReference type="EC" id="2.7.1.160"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the KptA/TPT1 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.160" evidence="3"/>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76738.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321157.2">
        <property type="nucleotide sequence ID" value="XM_003321109.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXG3"/>
    <dbReference type="FunCoup" id="E3JXG3">
        <property type="interactions" value="29"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JXG3"/>
    <dbReference type="GeneID" id="10528759"/>
    <dbReference type="KEGG" id="pgr:PGTG_02199"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02199"/>
    <dbReference type="eggNOG" id="KOG2278">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_052998_1_1_1"/>
    <dbReference type="InParanoid" id="E3JXG3"/>
    <dbReference type="OrthoDB" id="419694at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000215">
        <property type="term" value="F:tRNA 2'-phosphotransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.170.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.970">
        <property type="entry name" value="RNA 2'-phosphotransferase, Tpt1/KptA family, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002745">
        <property type="entry name" value="Ptrans_KptA/Tpt1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042081">
        <property type="entry name" value="RNA_2'-PTrans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042080">
        <property type="entry name" value="RNA_2'-PTrans_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12684">
        <property type="entry name" value="PUTATIVE PHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12684:SF2">
        <property type="entry name" value="TRNA 2'-PHOSPHOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01885">
        <property type="entry name" value="PTS_2-RNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56399">
        <property type="entry name" value="ADP-ribosylation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP76738.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="275" mass="30333" checksum="619B052867D5F1AC" modified="2012-04-18" version="2">MIRRGSPFKHFITTTPTQPPMIGSEPGTATQPSSAAHPPPPSSQAKPTAINNENNSKPKKTRGRPADSPDVTLSKTLSYILRHGALKERLVIRADGCIRLDDLLKRPKLKNYTIDDVRRVVAENEKQRFALVDEQAADGSIVNYIRANQGHSIKEVDQPDLTPLIDPIELPTVVHGTYSKFWDSIAQDGLKPMSRTHIHFAKGLLGEEGVISGMRASCDTFIYLDVEKCLQAQIEFFLSSNGVILSEGLPASKSIPAQYFKKVVDKHGTVLYPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXS6</accession>
    <name>E3JXS6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein required for ethanol metabolism</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_02312</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the peroxisomal membrane protein PXMP2/4 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76851.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321270.2">
        <property type="nucleotide sequence ID" value="XM_003321222.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXS6"/>
    <dbReference type="FunCoup" id="E3JXS6">
        <property type="interactions" value="252"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JXS6"/>
    <dbReference type="GeneID" id="10528956"/>
    <dbReference type="KEGG" id="pgr:PGTG_02312"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02312"/>
    <dbReference type="eggNOG" id="KOG1944">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_049109_8_1_1"/>
    <dbReference type="InParanoid" id="E3JXS6"/>
    <dbReference type="OrthoDB" id="430207at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007248">
        <property type="entry name" value="Mpv17_PMP22"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266">
        <property type="entry name" value="PEROXISOMAL MEMBRANE PROTEIN 2, PXMP2 MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11266:SF17">
        <property type="entry name" value="PROTEIN MPV17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04117">
        <property type="entry name" value="Mpv17_PMP22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU363053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP76851.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="194" mass="21967" checksum="8C684612E73C29D2" modified="2012-04-18" version="2">MAAFIRSYNHALLHRPLRTQIVTSLILFGGGDIIAQQAIERKGKQHEWARTARLAGYGGFVFAPLGTRWFKTLDFIQLKSRGLTTFLKLSIDQLIAAPTMLAFFFTTMNYLEGKDLKQAEERLREKWGPTLYKNWIVFIPLQAINFGLVPSHLRLLVINGASLFWNSYLSYANASTLPIVGKIIEQADHKLEAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3JYS8</accession>
    <name>E3JYS8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Kri1-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_03159</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the KRI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77203.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321622.2">
        <property type="nucleotide sequence ID" value="XM_003321574.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JYS8"/>
    <dbReference type="FunCoup" id="E3JYS8">
        <property type="interactions" value="251"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JYS8"/>
    <dbReference type="GeneID" id="10537579"/>
    <dbReference type="KEGG" id="pgr:PGTG_03159"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03159"/>
    <dbReference type="eggNOG" id="KOG2409">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009647_2_0_1"/>
    <dbReference type="InParanoid" id="E3JYS8"/>
    <dbReference type="OrthoDB" id="10252032at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030686">
        <property type="term" value="C:90S preribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000447">
        <property type="term" value="P:endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018034">
        <property type="entry name" value="Kri1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024626">
        <property type="entry name" value="Kri1-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14490:SF5">
        <property type="entry name" value="PROTEIN KRI1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14490">
        <property type="entry name" value="ZINC FINGER, ZZ TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05178">
        <property type="entry name" value="Kri1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12936">
        <property type="entry name" value="Kri1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Kri1-like C-terminal" evidence="3">
        <location>
            <begin position="553"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="56"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="104"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="347"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="486"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="612"/>
            <end position="696"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="64"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="137"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="178"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="370"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="527"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="623"/>
            <end position="639"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="645"/>
            <end position="670"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="671"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP77203.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="696" mass="80451" checksum="FD354C7DBA0A6821" modified="2012-04-18" version="2">MAPIDLFEDVEEEKQCRSGERQQSNRKNDSDAFHYNEAYAQKFERQKRGEELMKLKDKYGSDYEPSESEEDFSSEDSDAEFITPEVDVAILKTLARIKKEDPKLYEQDGNVFEEEEAQNAAARAQKLKRDKTESTNRKKPMNLRDYHRKALLTNEGREFDEDEPLPENYEPTPAEEAEALRDETKRAFHSLVDDDNHDDNESTKSSDGIQDMLVLRDKEVEEIEEEDQEYQRFLIEQVGNDDIQTALQLNFGTSNDASEQTEEKRKVRHDDDEFLRAYVLGRGWIDKEAKKIPKYKELVEAGGQSKQAKSNLSGSNANVLGKTAICDEYDEEDEEFVEKAEEFETNYNFRFEDPNPTVTTHARDTGPSARRKDDSRKLGRDAVKARKDEEKRQRMEELERMKALKKDEVLKKLEIIRKNAGAEDCNLDDIDLDSDFDPESHDRRMQQVFDNDFYGKTDPDGKPVWDDDIEIDDILPDAAPVQEEINFEPGGDAYDPLAPGGKKLSKKMKRLAKAEKKRGVVQNMEPEKEEEGPDHTEEVDDLEGLSPEERKRKLLEALDEYHKLDCEDMIGDMPTRFHYTKTEATDWMTPEQILMATDAELNQVIGLKKLAPYRSTDSDRKLATRKKKLKELRKKLKTRKWGEAHSNNDNPQASTSQPVTHDQNESQQPPTKKRKGKKERQKLKEEQTASNQVADS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZ16</accession>
    <name>E3JZ16_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03247</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77291.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321710.2">
        <property type="nucleotide sequence ID" value="XM_003321662.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZ16"/>
    <dbReference type="GeneID" id="10537836"/>
    <dbReference type="KEGG" id="pgr:PGTG_03247"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03247"/>
    <dbReference type="HOGENOM" id="CLU_030121_0_0_1"/>
    <dbReference type="InParanoid" id="E3JZ16"/>
    <dbReference type="OrthoDB" id="2503472at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="204"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="224"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77291.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="447" mass="50776" checksum="EE87F09B05224AE9" modified="2012-04-18" version="2">MSISVPPPISPSAPGSTSAPITTTTAAAEFTPIATTSASLAIDPEFTHKFCSACRKNVEIANFSTNRKICNRHSSQPIGAASVASPESALYQMRAMKFCQGVPHRTEQKKVLDIYVDYDALLDWLDQRKDIDPRVDRVRRIPKENDDELINDPNGQITLARRHATREARRVRDFIFIGTGLLFIHKTVAVGQKLQWTCQSRCSQSDRLKGGRDGSRRHSDSKRPSTSRTRYPCQGTTTVSLLHQQPGQRRLLHLVIKHGYVHGPHDDRNREPTLNEFMPETDFQVIDPALRNCPVNEPVKPVGSSSTKDDRLLKSSSSHDIFVDHQLLDHQLQEDQHEAVLKELNHLLAEDESRNKLIKKLMLDQQLQLQLQASTDLSLDNHHHHHHHHHHHLISSKLPILRDFFHKRAQLMVDLDSIIQSDAHKTHVDHLDHNIDVSMVMDSYPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3K089</accession>
    <name>E3K089_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03670</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77714.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322133.2">
        <property type="nucleotide sequence ID" value="XM_003322085.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K089"/>
    <dbReference type="GeneID" id="10539086"/>
    <dbReference type="KEGG" id="pgr:PGTG_03670"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03670"/>
    <dbReference type="HOGENOM" id="CLU_2265056_0_0_1"/>
    <dbReference type="InParanoid" id="E3K089"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="43"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="60"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77714.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="103" mass="11350" checksum="C95E3F598185F458" modified="2012-04-18" version="2">MDSEAELTDKEVSSLFESVRTEHPPGSTCWDQQNHLNQWMYGQRHRQSRVKHLTSLPGGQANSASKGATPRVSNNSSARTSSRGSALTQVSCKSREITARFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3K0C8</accession>
    <name>E3K0C8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Yeast cell wall synthesis Kre9/Knh1-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_03709</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322172.1">
        <property type="nucleotide sequence ID" value="XM_003322124.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K0C8"/>
    <dbReference type="GeneID" id="10540575"/>
    <dbReference type="KEGG" id="pgr:PGTG_03709"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03709"/>
    <dbReference type="HOGENOM" id="CLU_099094_0_1_1"/>
    <dbReference type="InParanoid" id="E3K0C8"/>
    <dbReference type="OMA" id="TPTNWQS"/>
    <dbReference type="OrthoDB" id="5420143at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052479">
        <property type="entry name" value="GPI-anchor_Adhesion_Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018466">
        <property type="entry name" value="Kre9/Knh1-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35185">
        <property type="entry name" value="SERINE/THREONINE-RICH PROTEIN ADG2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35185:SF1">
        <property type="entry name" value="UPF0619 GPI-ANCHORED MEMBRANE PROTEIN C1322.10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10342">
        <property type="entry name" value="Kre9_KNH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171667" description="Yeast cell wall synthesis Kre9/Knh1-like N-terminal domain-containing protein" evidence="3">
        <location>
            <begin position="20"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Yeast cell wall synthesis Kre9/Knh1-like N-terminal" evidence="4">
        <location>
            <begin position="24"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="109"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="120"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF10342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP77753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="207" mass="20975" checksum="0EE6E7B530891383" modified="2011-01-11" version="1">MRSLNLLISLAGLIASAAAFTINSPSASSYWVQFATNTIAWTNAPNDSPQVTLQIVNSNHTLLNGVFSIAEYVPAALEAYTVTNVTLVVADGYIVQMVNPANSSQVYASSSPFSVKPSDTPAAPGSGPGAPSSLSGLPGTSNDMRNMTGMSGMPGNNMSGRPGNTTGFSNNANGTANSNARSDASIVSPISFAVLSVVASAIGLATL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3K0P0</accession>
    <name>E3K0P0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Mechanosensitive ion channel protein Msy1/2-like transmembrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_03821</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77865.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322284.2">
        <property type="nucleotide sequence ID" value="XM_003322236.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K0P0"/>
    <dbReference type="GeneID" id="10540850"/>
    <dbReference type="KEGG" id="pgr:PGTG_03821"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03821"/>
    <dbReference type="HOGENOM" id="CLU_1031107_0_0_1"/>
    <dbReference type="InParanoid" id="E3K0P0"/>
    <dbReference type="OrthoDB" id="544685at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058650">
        <property type="entry name" value="Msy1/2-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25886">
        <property type="entry name" value="Msy1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="171"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="204"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel protein Msy1/2-like transmembrane" evidence="3">
        <location>
            <begin position="155"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP77865.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="270" mass="30673" checksum="04187EE84AB458E6" modified="2012-04-18" version="2">MRTSDEHDFRPTASRVSFEDDPPRQPPPVNPSHQQLQPATRNGSTTQLPSSNSSEYAEDSRSLTNRYQKALGLADTSLSTSTRTTKSQNLDSRQKILGVGAEVFSSTDDDDFDWDDSGESELDEAEREERQQRKLRAHHDHLEHENHLRRAKRLRKVYLCFMRLSRPIRTALLLVLGCSITITPAIVVILCYPQNRAKIHVLMWSIWCSINIALSCIISILVDIFPVIAIKLSCLFYGSAPEHLKTQVELWMGVRTYTKIALTVARPESK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>E3K1D6</accession>
    <name>E3K1D6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Tubulin gamma chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_04067</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers. Gamma-tubulin is a key component of the gamma-tubulin ring complex (gTuRC) which mediates microtubule nucleation. The gTuRC regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments, a critical step in centrosome duplication and spindle formation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Microtubule organizing center</location>
            <location evidence="1">Spindle pole body</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the tubulin family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP78111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322530.1">
        <property type="nucleotide sequence ID" value="XM_003322482.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K1D6"/>
    <dbReference type="SMR" id="E3K1D6"/>
    <dbReference type="FunCoup" id="E3K1D6">
        <property type="interactions" value="335"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K1D6"/>
    <dbReference type="GeneID" id="10541581"/>
    <dbReference type="KEGG" id="pgr:PGTG_04067"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04067"/>
    <dbReference type="eggNOG" id="KOG1374">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015718_1_0_1"/>
    <dbReference type="InParanoid" id="E3K1D6"/>
    <dbReference type="OMA" id="HRYISIL"/>
    <dbReference type="OrthoDB" id="10249382at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000931">
        <property type="term" value="C:gamma-tubulin ring complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005874">
        <property type="term" value="C:microtubule"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005819">
        <property type="term" value="C:spindle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005816">
        <property type="term" value="C:spindle pole body"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140490">
        <property type="term" value="F:microtubule nucleator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031122">
        <property type="term" value="P:cytoplasmic microtubule organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000212">
        <property type="term" value="P:meiotic spindle organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007020">
        <property type="term" value="P:microtubule nucleation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000278">
        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000070">
        <property type="term" value="P:mitotic sister chromatid segregation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007052">
        <property type="term" value="P:mitotic spindle organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02188">
        <property type="entry name" value="gamma_tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.600:FF:000004">
        <property type="entry name" value="Tubulin gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000003">
        <property type="entry name" value="Tubulin gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000045">
        <property type="entry name" value="Tubulin gamma chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.600">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002454">
        <property type="entry name" value="Gamma_tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000217">
        <property type="entry name" value="Tubulin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023123">
        <property type="entry name" value="Tubulin_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017975">
        <property type="entry name" value="Tubulin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11588">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03953">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01164">
        <property type="entry name" value="GAMMATUBULIN"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01161">
        <property type="entry name" value="TUBULIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00227">
        <property type="entry name" value="TUBULIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="7 9" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 9" id="KW-0493">Microtubule</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="11">
        <location>
            <begin position="66"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="12">
        <location>
            <begin position="267"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="32"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018848"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP78111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="483" mass="54123" checksum="1CE435670F3BC5FE" modified="2011-01-11" version="1">MPRELITISAGQAGNQISSEFWPQICAEHGISPTGLLQPPPQPSSSSGPTNNNPFQTNDCSAIDRKDVFFYQADDDHYIPRAIMIDLEPRVINTILTSEYSKLYNPENVYLSKDGGGAGNNWAAGYETGEKIYEEVMDMLDREADGSDSLEGFMLLHSIAGGTGSGLGSYLLERLSDSYPKKLVQTYSVFPHSDDSSDVVVQPYNSILAIKRLVNHSDSVVVLDNAALARIVSDKLHVSSPTWQQTNRLVSTVMSAATTTLRYPGYMNNDLVGMIASLIPTPRCHFLMTSYTPFTSERDGQEEAKSVRKTTVLDVMRRLLQPKNRMVSTGATINKSACYISILNIIQGEVDPTDVHKSLLRIRERHLANFIPWGPASIQVALTKKSPYIQTQHRVSGLMLANHTSIASLFKRTLDQYDRLRKRNAFLDQYKKTNMFANSWDEFDESREVLVDLIDEYRAAESKDYVNYTAPKSSLEDDRTHQR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3K1P6</accession>
    <name>E3K1P6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF7872 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_04177</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP78221.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322640.2">
        <property type="nucleotide sequence ID" value="XM_003322592.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K1P6"/>
    <dbReference type="GeneID" id="10542013"/>
    <dbReference type="KEGG" id="pgr:PGTG_04177"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04177"/>
    <dbReference type="HOGENOM" id="CLU_030195_3_0_1"/>
    <dbReference type="InParanoid" id="E3K1P6"/>
    <dbReference type="OrthoDB" id="2501761at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057194">
        <property type="entry name" value="DUF7872"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33339">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33339:SF1">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25278">
        <property type="entry name" value="DUF7872"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="signal peptide" evidence="3">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173467" description="DUF7872 domain-containing protein" evidence="3">
        <location>
            <begin position="29"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="149"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7872" evidence="4">
        <location>
            <begin position="307"/>
            <end position="522"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="244"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF25278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP78221.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="522" mass="56663" checksum="0DFBC999621095C9" modified="2012-04-18" version="2">MRYHTCLTVASSTLIISGLLVLAGPLQPRNLPAPPPAPPEDQCPKDIPLTPETWKKFEMDEFIAGMEGADKMTYIDFARKDSAHNDNIPFQLCNPVAGKNWLVLVAIQHWNTYLNVLYQGVANAVTIVRDASAEMITDFIPDTVMDKSIYGWAIATLVIGVLGGFSGALTPLLITKPVAQAAGEYGTATSIGSAAASNFQKTQEMQIAGDVVGVYRARSAHDAAIVSKAFEEAAKKAKPLPPMKQTTSSKSLAISSAPKTTAADHKKRSIMDENHANSVNKSPILYNIRPSTYPSQPKTLQKRGGPPPSAYAYQRWAEIDTHLAALQNRLQKDIAAVSHAGLTEPIYNNNGLAKNLLGGSMFEKFPTQIDEEEKNKAFAKITALNEIFKAQNMFITLGCDGCGDVGPGGAWPQDKFLSYCTPDGSMRNIIRAEGIHARNEVRNAQLLQSKYGFTTEYLTTRAINCQNKYGFYTLGKAPEPTNVDSDCAFSIPVCDCSIHVVHKKRKKGKGTVRACRAAGVPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3K370</accession>
    <name>E3K370_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_04883</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323346.1">
        <property type="nucleotide sequence ID" value="XM_003323298.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K370"/>
    <dbReference type="GeneID" id="10537115"/>
    <dbReference type="KEGG" id="pgr:PGTG_04883"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04883"/>
    <dbReference type="HOGENOM" id="CLU_1054258_0_0_1"/>
    <dbReference type="InParanoid" id="E3K370"/>
    <dbReference type="OMA" id="KICFSEM"/>
    <dbReference type="OrthoDB" id="2500097at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173200" evidence="2">
        <location>
            <begin position="19"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP78927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="265" mass="29290" checksum="12CE966FE9BF3AA8" modified="2011-01-11" version="1">MRVSHLLILSAFLSLVNTLGISNQSRVFLRRSTFPKQVFPYRQASIKKARQIDLSTPRSPTVSLKPSVTSTAPTAPPPRSNSRQNTRSNSILKKKFYAYFHECNIGVRVHVQRIKKICSGKITNESAKKISWELLVELQAILALCLTCLRKIKSCGKAPIPSSLPGISLNSPSLRDLGQIVLQLLNQLKICFNEMNIVCSRYEIIRTTCSDCLVHISSSISGCLYASNAHINGFLATVTNLLGGNPLSFFGLDKFGLDSIVKILQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3C1</accession>
    <name>E3K3C1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Vacuolar protein sorting-associated protein 62</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04934</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78978.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323397.2">
        <property type="nucleotide sequence ID" value="XM_003323349.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3C1"/>
    <dbReference type="GeneID" id="10537166"/>
    <dbReference type="KEGG" id="pgr:PGTG_04934"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04934"/>
    <dbReference type="HOGENOM" id="CLU_024079_1_0_1"/>
    <dbReference type="InParanoid" id="E3K3C1"/>
    <dbReference type="OrthoDB" id="188042at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009291">
        <property type="entry name" value="Vps62"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48171">
        <property type="entry name" value="DUF946 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48171:SF1">
        <property type="entry name" value="VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06101">
        <property type="entry name" value="Vps62"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173507" description="Vacuolar protein sorting-associated protein 62" evidence="1">
        <location>
            <begin position="33"/>
            <end position="411"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78978.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="411" mass="46249" checksum="4FBF1E1B8E8B88D7" modified="2012-04-18" version="2">MLLNSHHTSSLLTGNIILILALLSPCLDEVLSTTIPNTPQLPYFTSKQTLPQYALDFAPLVYLHSQEVFWPSDLNRHLDNVQPQINFTTVKSYLRETQTTQDFLGSSNLQRISPSQQEITYLSSKNPIFDLDYASWLRGAGPPDKRGRSSACAIIIAVDKTQQVGPGWVDVFYLFFYSYNRGNYFNGNRFGDHVGDWENTMIRFKNGKPMYVAPEAHGGRVMLGDSAFNYNVLEHLNGRPVVYAANGTHGMYPQPGQQNYTGLPVAIYDVTDKGHLWDPTLNYKSFFFSNEHGFSYTADTPLKLQSRQRLGWLRFLGYWGDQKPELSNPHQTCIGTMCFYEGGPLGPQSKSLSRGWICGSSDCQPQSTLPTMFRPGGQRANSPYSSSWTNRLNFLSGVLLTFLTAYAIHLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3C7</accession>
    <name>E3K3C7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Diphthine--ammonia ligase</fullName>
            <ecNumber evidence="2">6.3.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Diphthamide synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Diphthamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_04940</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>diphthine-[translation elongation factor 2] + NH4(+) + ATP = diphthamide-[translation elongation factor 2] + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19753"/>
            <dbReference type="Rhea" id="RHEA-COMP:10172"/>
            <dbReference type="Rhea" id="RHEA-COMP:10174"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16692"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:82696"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.1.14"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.14" evidence="2"/>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323403.1">
        <property type="nucleotide sequence ID" value="XM_003323355.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3C7"/>
    <dbReference type="FunCoup" id="E3K3C7">
        <property type="interactions" value="26"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K3C7"/>
    <dbReference type="GeneID" id="10537172"/>
    <dbReference type="KEGG" id="pgr:PGTG_04940"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04940"/>
    <dbReference type="eggNOG" id="KOG2316">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2317">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010289_2_1_1"/>
    <dbReference type="InParanoid" id="E3K3C7"/>
    <dbReference type="OMA" id="HCRLAQS"/>
    <dbReference type="OrthoDB" id="686384at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017178">
        <property type="term" value="F:diphthine-ammonia ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01994">
        <property type="entry name" value="AANH_PF0828-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd06156">
        <property type="entry name" value="eu_AANH_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.40:FF:000010">
        <property type="entry name" value="Diphthine--ammonia ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000194">
        <property type="entry name" value="Diphthine--ammonia ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1490.10:FF:000001">
        <property type="entry name" value="Diphthine--ammonia ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.40:FF:000012">
        <property type="entry name" value="diphthine--ammonia ligase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1490.10">
        <property type="entry name" value="putative n-type atp pyrophosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.40">
        <property type="entry name" value="RutC-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002761">
        <property type="entry name" value="Diphthami_syn_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030662">
        <property type="entry name" value="DPH6/MJ0570"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035959">
        <property type="entry name" value="RutC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006175">
        <property type="entry name" value="YjgF/YER057c/UK114"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00290">
        <property type="entry name" value="MJ0570_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196:SF2">
        <property type="entry name" value="DIPHTHINE--AMMONIA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196">
        <property type="entry name" value="DOMAIN OF UNKNOWN FUNCTION 71 DUF71 -CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01902">
        <property type="entry name" value="Diphthami_syn_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01042">
        <property type="entry name" value="Ribonuc_L-PSP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55298">
        <property type="entry name" value="YjgF-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="domain" description="Diphthamide synthase" evidence="12">
        <location>
            <begin position="37"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Diphthamide synthase" evidence="12">
        <location>
            <begin position="124"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="319"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="319"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP78984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="716" mass="78790" checksum="56756D8CD82866FD" modified="2011-01-11" version="1">MRLRIHEPFGLDTLQPRRQLLYLSCSLFEVYVLLALMKVVGLLSGGKDSCYNLCHCVKNGHDIVALATLGPEAGTDELDSYLYQTVGQDGIHLVAEALRLPLYRQTIKGTPVELGSEYGSRCHKSLMKGVEGDETEDMYNLLNRVKSIHPEITAVSVGAILSSYQRVRVEYVCQRLGLTVLAYLWQRDQAELLREMVEASVESVLIKIAGVGLMPDHLGKSLAEMEPILTTLNSKYGAHVCGEGGEYETCTLDCPLFYSRISLEKTLLTHHNESSNIAPVAYLRLLSAKLSPKPSSVPDLNAVTVPPLLDAESVKTKLQVGTHQPPSLQRTRTSLRDTTNRQSSVSECGDWVVIASIFGSSSSISPDNIEQTLENEVEEVFNNLEVLLAKSSLTFVDIAHINLHLSSMAYFSEVNRVYSKKFGTSPPTRACVANPLPGTARVMLDAIVRRPATDLHHRDRVALHVQSRSYWAPANIGPYSQAVMVGSKIFVSGQIGLVPATLTLPVPRSFMEEAVLSLQHARRILATFPGPQWIESIVCYLTEISHLEQARMVWQHTSSIHHIHIPVLFLEVSELPKGALIEWQVVAGTDQSSADQDQDQDELVVPGPAYVSLGQPTFYACHSRPSRALTVIGTLPKDPVVGIELPPHHATYIRGFHTARISAVEAERRIRDSFGSRQDQKVDDCALSLSCVNSIGLNTGAADLDIGFYMMGVLSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3I5</accession>
    <name>E3K3I5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04998</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP79042.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003323413.2"/>
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    <dbReference type="AlphaFoldDB" id="E3K3I5"/>
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    <dbReference type="KEGG" id="pgr:PGTG_04998"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04998"/>
    <dbReference type="HOGENOM" id="CLU_005726_6_0_1"/>
    <dbReference type="InParanoid" id="E3K3I5"/>
    <dbReference type="OrthoDB" id="2505908at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35871:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35871">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="191"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="204"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79042.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="581" mass="65891" checksum="1213C81A88061178" modified="2012-04-18" version="2">MTGKKVSKAKKGQRRWRRREKIEKEDKEAKSVKIKTKEPKQASKIKTKNSRKDKISAPTPILVDSDSDINVVHLIEDENDAEVQILNAGPINSDKIQILKDRAMVHGQPTQTEPSINNSEPEKNTETTDMELFDFQEAEIEARNQGNDLVNYYDLAMDDETSDEDSDETEDKDKRFEGLWPIFSGGISASQESIKHRQLKSGKKTYQQPVKNPNSSSSKLIPAPIPKQTKHDRKNRRIQALGTNNTILENYFIRLEPVKPAIADSAIDPDLVSATLLNSTIANPCLDQAERESVDAQLACLLEQRLAAPTKPQTASKSDKAQAKWEALNSALNKATLRYQQKVKKDKKFKFPQSMMNNLYQFNNLRLKYSLNGTGGLITPSVTASLAAAQSSIEQHPSTTGPPKLRSGIYLSWSIRREAKHILNNQHLSEVKQGNRKNHRLLLENVELRKVLFTWAASQVAGHVTPITFREHVINKVFPQFDIQEGLSRNAVTRWMIKLGYRPQEYRKCLYFDGHERPDVLKSRKKYIEDFSSYRKQSRVYGGDDLETAAQVDPEALGDGKETVFIFHDESTVVRPNCSRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3M3</accession>
    <name>E3K3M3_PUCGT</name>
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        <recommendedName>
            <fullName evidence="1">Amidohydrolase 3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05036</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP79080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323499.1">
        <property type="nucleotide sequence ID" value="XM_003323451.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3M3"/>
    <dbReference type="GeneID" id="10530901"/>
    <dbReference type="KEGG" id="pgr:PGTG_05036"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05036"/>
    <dbReference type="HOGENOM" id="CLU_2062625_0_0_1"/>
    <dbReference type="InParanoid" id="E3K3M3"/>
    <dbReference type="OrthoDB" id="3501663at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013108">
        <property type="entry name" value="Amidohydro_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22642">
        <property type="entry name" value="IMIDAZOLONEPROPIONASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22642:SF2">
        <property type="entry name" value="PROTEIN LONG AFTER FAR-RED 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07969">
        <property type="entry name" value="Amidohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase 3" evidence="1">
        <location>
            <begin position="10"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="119" mass="13291" checksum="DD16A448AB2FDB25" modified="2011-01-11" version="1">MRMGAEAWLGSELRIRGAYAWNSLLKKNVRLALGSDFPVSSVSPFLGIHAAFTQRKAGPSDNDDHQGWYPSKRIESLQQIIDGFAQSMLVLLASLIISLEVYALENWHGQFCDHQSESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3S9</accession>
    <name>E3K3S9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04687</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78731.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323150.2">
        <property type="nucleotide sequence ID" value="XM_003323102.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3S9"/>
    <dbReference type="GeneID" id="10536668"/>
    <dbReference type="KEGG" id="pgr:PGTG_04687"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04687"/>
    <dbReference type="HOGENOM" id="CLU_860891_0_0_1"/>
    <dbReference type="InParanoid" id="E3K3S9"/>
    <dbReference type="OrthoDB" id="10366909at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="183"/>
            <end position="206"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78731.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="323" mass="35965" checksum="9A1A003A41B5FCF0" modified="2012-04-18" version="2">MLRVESLLSSIPVGADPYDYIKHSILKWGSVPLCRAALVSLVVILILHAFIAATCLLLLTLPCIRPLKPSPWYLRKLYIEDHSSGKAHQVPLYFANAGVLLTTSQLLSSASTSAFIWFDIGAALPGEHLFGSRALPAVGLMNLAETFAYWSLSHCFLVITICHGNKKQQEKSMASIRHIPPQLINFIFTFVPIAAMVITIFVIAWVTQVTSEIQSYTKQGLTALDQGSSIWKQLQVTPQSLEAQAQLRGITMQLASLFHNAFPRLEVAFYRFRLGECLFLSFESISCPTSRKIALGITHEARSWAHIQIRSRYLSLRSGYSSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>E3K419</accession>
    <name>E3K419_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">MHD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_04777</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <location evidence="2">Coated pit</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the adaptor complexes medium subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78821.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323240.2">
        <property type="nucleotide sequence ID" value="XM_003323192.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K419"/>
    <dbReference type="FunCoup" id="E3K419">
        <property type="interactions" value="293"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K419"/>
    <dbReference type="GeneID" id="10536841"/>
    <dbReference type="KEGG" id="pgr:PGTG_04777"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04777"/>
    <dbReference type="eggNOG" id="KOG0938">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026996_5_2_1"/>
    <dbReference type="InParanoid" id="E3K419"/>
    <dbReference type="OrthoDB" id="10259133at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030122">
        <property type="term" value="C:AP-2 adaptor complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031410">
        <property type="term" value="C:cytoplasmic vesicle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09251">
        <property type="entry name" value="AP-2_Mu2_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd14836">
        <property type="entry name" value="AP2_Mu_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000002">
        <property type="entry name" value="AP-2 complex subunit mu, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1170">
        <property type="entry name" value="Mu homology domain, subdomain B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050431">
        <property type="entry name" value="Adaptor_comp_med_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036168">
        <property type="entry name" value="AP2_Mu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043532">
        <property type="entry name" value="AP2_Mu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001392">
        <property type="entry name" value="Clathrin_mu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018240">
        <property type="entry name" value="Clathrin_mu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028565">
        <property type="entry name" value="MHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043512">
        <property type="entry name" value="Mu2_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10529">
        <property type="entry name" value="AP COMPLEX SUBUNIT MU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00928">
        <property type="entry name" value="Adap_comp_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005992">
        <property type="entry name" value="Clathrin_mu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00314">
        <property type="entry name" value="CLATHRINADPT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49447">
        <property type="entry name" value="Second domain of Mu2 adaptin subunit (ap50) of ap2 adaptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00990">
        <property type="entry name" value="CLAT_ADAPTOR_M_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00991">
        <property type="entry name" value="CLAT_ADAPTOR_M_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51072">
        <property type="entry name" value="MHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0168">Coated pit</keyword>
    <keyword evidence="5" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="domain" description="MHD" evidence="10">
        <location>
            <begin position="169"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR005992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP78821.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="432" mass="49000" checksum="E78F1B80E10B5716" modified="2012-04-18" version="2">MISALFILNQKGEVLISKLFRPDLKRSIADIFRIHVISNPDVRSPIITLGSTSFFHVRHQNLYLTAVTKTNANAAIVFELLYRIINIAKSYFGKVDEEAIKNNFVMIYELLDEILDFGYPQNSEIDTLKMYITTESIKSEQAVREDSAKITIQATGATSWRRNDVKYRKNEAFVDVVETVNLIMSSKGTVLRADVDGQILMRAYLSGTPECKFGLNDKLIIERTDRAKPSGSTRTDESAVELDDCQFHQCVKLGKFDSDRTISFIPPDGEFELMRYRSTTNVQLPFRVHPIVEEIGKSRVEFTVHLKANFDSKLNANSVVVKIPTPLNTTKVACKAQIGKAKYVPEENVIIWKIPRMQGQSDATITASADLSATTHRKAWSRPPINIDFQVLMYTSSGLLVRFLKVFEKSNYNSVKWVRYLTKASGSYQIRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3K498</accession>
    <name>E3K498_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Metal homeostatis protein bsd2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_05429</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79108.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323527.2">
        <property type="nucleotide sequence ID" value="XM_003323479.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K498"/>
    <dbReference type="FunCoup" id="E3K498">
        <property type="interactions" value="106"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K498"/>
    <dbReference type="GeneID" id="10530931"/>
    <dbReference type="KEGG" id="pgr:PGTG_05429"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05429"/>
    <dbReference type="eggNOG" id="KOG4812">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_016313_0_0_1"/>
    <dbReference type="InParanoid" id="E3K498"/>
    <dbReference type="OrthoDB" id="10003116at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048471">
        <property type="term" value="C:perinuclear region of cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
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    </dbReference>
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            <begin position="363"/>
            <end position="384"/>
        </location>
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        </location>
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        </location>
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            <begin position="327"/>
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        </location>
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        </location>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP79108.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="404" mass="43234" checksum="38BEABD4D393D072" modified="2012-04-18" version="2">MVNMSSPLSSLRPGQPATQQSYSRLPNQQTDADGTNYFDQDDELDAAFNEPEEAGHHESRRMDEDDEDEDEEEDERHGLVSHQASASSSSKMILTDKTQVQPSRTYDFEPDSSSTRFHPNAGSPTPSLGPPSLVRLAGMGGGVQNDGVFANLSAKPDGPGARLNPSTGQMEFVGGNDDGHPTDKEEVLPTYDSAALDSAPPYWETTVTLPGVNSGPYGLLGPDDILVDGLPVGNLFGFAWNLLVSMSFQFIGFLLTYLLHTTHAAKNGSRAGLGITLIQYGLYTRTRALEGTNGVGGGASSRSSAYNSSSVPPTTTDPWGLFGPSSTSDWDGSTDRAAKGSQNMPYDGIPNNHGHVSGAANEWLGYILIAVGWFLLLGGLVNYWRAVRWARTVRNSDMLGEALA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3K5S1</accession>
    <name>E3K5S1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05813</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="E3K5S1"/>
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    <dbReference type="KEGG" id="pgr:PGTG_05813"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05813"/>
    <dbReference type="HOGENOM" id="CLU_1876454_0_0_1"/>
    <dbReference type="InParanoid" id="E3K5S1"/>
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    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
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    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="61"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79492.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="136" mass="14990" checksum="890FA84817E191C3" modified="2012-04-18" version="2">MPRGRKQPHSGNPATIASSTSKAATTTSSRYSRNSDSESDSESDVKISQDQPSVGITPRACSHDEQENAKRKACDLNHDVNDDMNSVNDKNVSLNSKSSVRHSNYQIVNTYTTKLANCISSSILAKRTARDQRRLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>E3K616</accession>
    <name>E3K616_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_05908</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79587.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324006.2">
        <property type="nucleotide sequence ID" value="XM_003323958.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K616"/>
    <dbReference type="GeneID" id="10531420"/>
    <dbReference type="KEGG" id="pgr:PGTG_05908"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05908"/>
    <dbReference type="HOGENOM" id="CLU_2062628_0_0_1"/>
    <dbReference type="InParanoid" id="E3K616"/>
    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP79587.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="119" mass="12724" checksum="9654DD42D5C8B5A8" modified="2012-04-18" version="2">MESPHRRFGLASLLLHHAPGGNWASAPLWHLQTGLRGGGSRVTSLSVTAWPSPGVDEGLSQLSCPGNALTKQPRRKNDVNFVNEFPVRLFVAQVTMTHGGYIPDRAITACNIGLAGAVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3K6H8</accession>
    <name>E3K6H8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_05232</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ORC4 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EFP80007.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324426.2">
        <property type="nucleotide sequence ID" value="XM_003324378.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K6H8"/>
    <dbReference type="STRING" id="418459.E3K6H8"/>
    <dbReference type="GeneID" id="10533861"/>
    <dbReference type="KEGG" id="pgr:PGTG_05232"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05232"/>
    <dbReference type="eggNOG" id="KOG2228">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_282147_0_0_1"/>
    <dbReference type="InParanoid" id="E3K6H8"/>
    <dbReference type="OrthoDB" id="343623at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005664">
        <property type="term" value="C:nuclear origin of replication recognition complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003688">
        <property type="term" value="F:DNA replication origin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041664">
        <property type="entry name" value="AAA_16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016527">
        <property type="entry name" value="ORC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032705">
        <property type="entry name" value="ORC4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12087">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12087:SF0">
        <property type="entry name" value="ORIGIN RECOGNITION COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13191">
        <property type="entry name" value="AAA_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14629">
        <property type="entry name" value="ORC4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orc1-like AAA ATPase" evidence="7">
        <location>
            <begin position="573"/>
            <end position="770"/>
        </location>
    </feature>
    <feature type="domain" description="Origin recognition complex subunit 4 C-terminal" evidence="8">
        <location>
            <begin position="819"/>
            <end position="1079"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="367"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="402"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="657"/>
            <end position="687"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="82"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="6">
        <location>
            <begin position="143"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="259"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="272"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="411"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="6">
        <location>
            <begin position="434"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="460"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="657"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="667"/>
            <end position="685"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005334"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF14629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP80007.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1108" mass="122840" checksum="643D9AB6B098B416" modified="2012-04-18" version="2">MTTFNLNLNLNQPTNQPKDIMPEPATLHHHHRPGSLTSSPNRISPRRRKNKPAPPTDLSLKTRPVRSLPTTQRVAVVIPRPSRSNTLTKKKSQTLSASTSAPNLKMPSRKRNCPEPLGRFDSDQQPEIIPRLRKKTRQSGPLTPAPTSPINPPDLEAESSEDEILLKPSTHTKPEPKTKPLQLPPSPTSSHNDSPVRSRAKTRSLTRQADQRQAGCESTPVKLRSKKSVHQTAPVNRALTDHSVKDPTSLKTRGRPRQRPLSPSPSSHRSPTNRLTKNSGHVQLLGPESDGDDSDGQATLTATSPLKAKLFKTRVQPHTPNPPRTSPRRAGPSAIDPTIMPDSENVDELLRQIESMIPKDSPILLNQASFQKQRAPDGSVEAKEDDLVSGVDERCFKVLEDAQLAERSDQSDNEDSQASETDDDQVAKELNQQLIPSSNRSPRRLSSVSPLEISAGTTTRLRRLTHHTKRPAKASSPDKALAKEPVAAPKTVASPAKTVVDKDGFPSSVVTPYLHDLLQHLSGVRPLSIRIPQALASSLSDPHKHDPSRIPTILDQTPCLVGLEDLEIELRSILFRSVSQQEENVLLLSGARGSGKTALISRSLSLLSDPLICGSEGFITIKLNGLVHNSDKVALKEMCRQLFSSLALSKDSDQSRKLDQVNRDLDFQPDDSADEDEEEDEDEVADSARRAAVVNQPRFTNYGETLKNLLDVLEPSSSLSIEHDPILARQSHQKKPQNNKALVIILEEFDLFSDLDRQSFLYCLLDSVQGNKRQNGICVIGTTSVVDCLDKLEKRVKSRCQSRIRYLHSPKTEPERIDLLRSLLELPGDHLPEASSASSPTKSVDQKRKTFVQKWNDSLGAFLDHAHTRDWLRSKFMLINDPLVQILQDLNGMVSMIAYQAKRTGQLKLPALNPSELFPTRPVGGGTGPTKPKVMRSSTLISTTLPWWADALSIAEFSVLIACKHLSSTHHNGIFNLEMAWDLYRQHLKRLSFALDAEPLSSDVRAKDQFRPAVVRSQVYGRDAFELAWERLQRLELILPLEVLNSKAVALGQKKKSSVILGKRYELVKLVPFLSQIDLVLLGSSSSQQLPRVGLAHHLLKWAKNSSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3K7I9</accession>
    <name>E3K7I9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">short-chain 2-methylacyl-CoA dehydrogenase</fullName>
            <ecNumber evidence="11">1.3.8.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PGTG_06436</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>2-methylbutanoyl-CoA + oxidized [electron-transfer flavoprotein] + H(+) = (2E)-2-methylbut-2-enoyl-CoA + reduced [electron-transfer flavoprotein]</text>
            <dbReference type="Rhea" id="RHEA:43780"/>
            <dbReference type="Rhea" id="RHEA-COMP:10685"/>
            <dbReference type="Rhea" id="RHEA-COMP:10686"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57336"/>
            <dbReference type="ChEBI" id="CHEBI:57337"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58307"/>
            <dbReference type="EC" id="1.3.8.5"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:43781"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Amino-acid degradation; L-isoleucine degradation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Lipid metabolism; mitochondrial fatty acid beta-oxidation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EC" id="1.3.8.5" evidence="11"/>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80480.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324899.2">
        <property type="nucleotide sequence ID" value="XM_003324851.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K7I9"/>
    <dbReference type="STRING" id="418459.E3K7I9"/>
    <dbReference type="GeneID" id="10539491"/>
    <dbReference type="KEGG" id="pgr:PGTG_06436"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06436"/>
    <dbReference type="HOGENOM" id="CLU_018204_0_1_1"/>
    <dbReference type="InParanoid" id="E3K7I9"/>
    <dbReference type="OrthoDB" id="10262177at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003995">
        <property type="term" value="F:acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003853">
        <property type="term" value="F:short-chain 2-methyl fatty acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006631">
        <property type="term" value="P:fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000004">
        <property type="entry name" value="Acyl-CoA dehydrogenase FadE25"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000012">
        <property type="entry name" value="Acyl-CoA dehydrogenase short/branched chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000001">
        <property type="entry name" value="Acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
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    </dbReference>
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        <property type="entry name" value="ACYL_COA_DH_1"/>
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    </dbReference>
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        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 13" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="16">
        <location>
            <begin position="62"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="15">
        <location>
            <begin position="177"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="14">
        <location>
            <begin position="286"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00005198"/>
        </source>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
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        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00039036"/>
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    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00048235"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EFP80480.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="424" mass="46695" checksum="5E1FBF7068E61251" modified="2012-04-18" version="2">MLTNPSRQVLLRTQQLLRSSKPTPRVLSSRHPTSALQRAFSSHPIARSQDGEPQPTSLYTFTEDESMLKDLAQRFAEEVVAPKVREMDEKETMDPEIIKGLFDNGFMGIETSQEHGGSGASFTSAILVIEELAKVDPSVSLICDVHNTIANTTIRNYASKALLDKYMPDLSTSKVACFCLSEPGSGSDAFALATRATKDSNGDYIINGSKMWITNSKEAEIFVVFANLDPSKGYKGISCFLLEKSMGVEIAKKEEKLGIKASSTCTLNFDNVKVPKENLIGQEGLGYKYAIEILNEGRIGIAAQMVGLAQGAFDKAVKYTYQRKQFGKPVGEFQGMQFQFADVHTEIEAARMLTYNAARLKEEGKPFTEMAAMAKLFSSQVAQNASGSAIEWCGGVGFTRETGIEKYWRDSKITLAKFIQKRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K8H2</accession>
    <name>E3K8H2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06710</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003325125.1"/>
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    <dbReference type="AlphaFoldDB" id="E3K8H2"/>
    <dbReference type="GeneID" id="10539773"/>
    <dbReference type="KEGG" id="pgr:PGTG_06710"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06710"/>
    <dbReference type="HOGENOM" id="CLU_026173_0_0_1"/>
    <dbReference type="InParanoid" id="E3K8H2"/>
    <dbReference type="OrthoDB" id="10297013at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="131"/>
            <end position="158"/>
        </location>
    </feature>
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        <location>
            <begin position="182"/>
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    </feature>
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        <location>
            <begin position="474"/>
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        </location>
    </feature>
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        <location>
            <begin position="131"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="478"/>
            <end position="489"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP80754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="503" mass="54076" checksum="F45C62EF4AB4B8BE" modified="2011-01-11" version="1">MTQQWSRRPFSPTENRYDSRIRYLEEVVHLLLAGTNSTCFSLSTPTLAGSFRYSIKRGLEPVLPDKTAMSLVADWRIGRPIRQKRFSSPPCRQNRLLATCNTSLHPARVPSPTPSTSLTNPASPATIGVADSTNTAKSSSTSNTKHQAAVNPMEVSASPTDSITTLGFAANTAQNYTPQLADPHSPPPVNHCSSPDSPVSHKRGLAGAFSFELPPPALAISLERPTSLEAHASHAITLTQRSNSANVVEPNNFSSLDNTAATATAVIDLINNNAIQNKGPSPFPPPSSSSSAHPSTTAIIIESHDQSSTLLVVNGPAPITSTATTSAPSRSYDHQLTFISATDAVIAPTSLSCLNLINNKDNQDNLLPTTNLAQSFTDTIDDNITSFPVTLPIDPTLPLPSVINNLDLVCHQTQPIKPPAQPDNFEVAAVGGIEILDNENSNAIDARLAPEYSQATLDYYNDIQEYLQQANTEGTEMKKKKKKKKKKKANLTSTPDNPILFYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K9J8</accession>
    <name>E3K9J8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07358</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP81106.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325525.2">
        <property type="nucleotide sequence ID" value="XM_003325477.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K9J8"/>
    <dbReference type="GeneID" id="10536428"/>
    <dbReference type="KEGG" id="pgr:PGTG_07358"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07358"/>
    <dbReference type="HOGENOM" id="CLU_011692_1_0_1"/>
    <dbReference type="InParanoid" id="E3K9J8"/>
    <dbReference type="OrthoDB" id="2507496at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="269"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="475"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="360"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="483"/>
            <end position="500"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81106.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="523" mass="57036" checksum="3B3002ED600FD0B0" modified="2012-04-18" version="2">MSHLKGRNDLDLDDLLGHLPFTNVEDAGQKLYLGEPKRESVPFETNLTTNSAASQPPQGCSSPTAKEWEKITPTFDTVWKAALEVWSWPNAQKCIITLLREGRPLVADHLVNLQENGLLKWQCILANHSTYGANKMYLVYSDEDFTPFVAAVLKKPGSKVTIKLLMDYPARSARRKQAEQSQDNSLAMSYAPNDERLALHRLQTESLHIKDPANPACSMRFTRDGLYVWSRALVHKAKGVTNKIPPVTKEFMLEPITIYTQAEKSALANRRGGKAQSARPAVSDDDPRSIPATPMGTINRPPGSRSSTDIGVWSADRSPARKIARSPGGGGISVGRLDYNTSGPSRGAPKTLPTRKIAGSNGTASVGSAGTSQVLSMGSGPSAMLSMGSAVLIPPRRSLPPLNDLGRALGMETFLDLCNFQRDDLVPHVLINMAHIRHWEFFYRNTNVIQLQRMGFPYPIASQLMNGAEYVGATHTEAPTDEPTDKAANDTRQDGPGKDNDQEDPTANLAGGNTQDPALSPEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KA95</accession>
    <name>E3KA95_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06873</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325671.1">
        <property type="nucleotide sequence ID" value="XM_003325623.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KA95"/>
    <dbReference type="GeneID" id="10536739"/>
    <dbReference type="KEGG" id="pgr:PGTG_06873"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06873"/>
    <dbReference type="HOGENOM" id="CLU_1998045_0_0_1"/>
    <dbReference type="InParanoid" id="E3KA95"/>
    <dbReference type="OrthoDB" id="10283239at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="133" mass="14068" checksum="5A1A086E8668EA07" modified="2011-01-11" version="1">MHQGKPPATTFPTKASPPTHVSSKSFNFRSSTLILVDLAETRTIMKCFTAIVVATLAAGTMAAPPVQDVVTGFESSQTQITGFGPKASYTVAPDAADGGKEPDHWTGMCTDGKCGDGTNCAIWCKNWTDKNKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KC12</accession>
    <name>E3KC12_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08052</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EFP81803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326222.1">
        <property type="nucleotide sequence ID" value="XM_003326174.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KC12"/>
    <dbReference type="GeneID" id="10533673"/>
    <dbReference type="KEGG" id="pgr:PGTG_08052"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08052"/>
    <dbReference type="HOGENOM" id="CLU_039964_2_0_1"/>
    <dbReference type="InParanoid" id="E3KC12"/>
    <dbReference type="OrthoDB" id="2501890at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="242"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="368"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="259"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="472" mass="52035" checksum="2DE850E76ACB0C6D" modified="2011-01-11" version="1">MDPSTEPSQCDSSADPSLPTYKQVITDHPSAKKAVKSAKLKKPRAAPKSKSKSKKKNKNISPSLDIASGDDEDNAVKKVIVTTNFKLFVLDVDRSKKAKKVWLLISPPNPIKIEITISPKDKVTTHDQFISLVTSKCENAVANTGAILSDGLSSGSPVINWFVSIARVEGFKKGKPFLLENQMAFNTWMEALADNEADESTLSIEMTNPNQVAKLQHDAEVLAKDAVRKEAKRLITVAREAKRARAGDSEGGKRKRLDEDSDEDTEGSDDEVEYDKDAVKLFMRQLYATHSANALYDRHLPVYINPLNHSQYFLLSNGTCQKWARALVKVREGVSIQSPPKEFKYETLPPANSIPTSTKQLARTSTPAGCAHHACSGHASHASPPSSSDGPQPGKDVGIRDYINFIGLRQADEVVDLLIRNDLQNFKIFRSRNLDRSALRSLGLTLGVVTQLCDYVSKFERHLAKQDLLASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KE01</accession>
    <name>E3KE01_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08506</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82550.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326969.2">
        <property type="nucleotide sequence ID" value="XM_003326921.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KE01"/>
    <dbReference type="GeneID" id="10529932"/>
    <dbReference type="KEGG" id="pgr:PGTG_08506"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08506"/>
    <dbReference type="HOGENOM" id="CLU_063939_0_0_1"/>
    <dbReference type="InParanoid" id="E3KE01"/>
    <dbReference type="OrthoDB" id="2511178at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82550.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="217" mass="23512" checksum="24E6CC024C1FEF4B" modified="2012-04-18" version="2">MTGDTPSALQQPSGFEGLGSSLPSVTGNAMRPQVAETLRSELGISPALINSRATSLALETASNRSTPVPCPVDRQVFPVNSTTATGRQILSPTPLLPNRNQFAALSPSPAPLGLDDIIVDNQELETVIHLFNKQWNTFVQARENQNLRLMRMALVQAISSQDEIRLLAGGAEMLRICENWIAREELADLERAEAASCHQTAQRLAITQTAHPRPLSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KFF9</accession>
    <name>E3KFF9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09954</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178284">
        <property type="protein sequence ID" value="EFP82986.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327405.2">
        <property type="nucleotide sequence ID" value="XM_003327357.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KFF9"/>
    <dbReference type="GeneID" id="10544697"/>
    <dbReference type="KEGG" id="pgr:PGTG_09954"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09954"/>
    <dbReference type="HOGENOM" id="CLU_031991_2_0_1"/>
    <dbReference type="InParanoid" id="E3KFF9"/>
    <dbReference type="OrthoDB" id="10272108at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="93"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="140"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="395"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="159"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82986.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="503" mass="55495" checksum="B3DE4077A783A0DD" modified="2012-04-18" version="2">MRFSINDLIHDKPPSPTSRLSSNDWMQDPFVQNRLTESNNPFSANDRNEGLSISSSATGFNIVNQFPFQDQQKFHSVHGPSVPAARIFDGTAHLQGPQNTSEPPAYQPVGQSNGIEAEQPRGNSPQIPIQIEFTFYLATESPARPSNPRKRPPPASNEPRTEKKVHSDPDTIRIYWDAQDRNFERFKDTVIQAIGEKEGEQLAIFARTQDSAGNIDWCVSIPFGGLFAANHKRRLENSKVFARFITAAEGASDDRKVLCQLVKKDPKAIAEKASALKRLKQTQSGLNPDGLGNNPEVTPTASESSGAEITRLIRDILTAHDPCKQLSGSSEKPVFINPENQNELFVITFPKADAWARAIMSLNKPDSPQVFHLLLNQAPLQVNRLIQLHRSNTLRTSAPSGLSNPVASTSTAQPSEAPVIKSSPAPSDGTSNLEDFLKFARGNGDSVALNEGLNELGITHWSMFQLTNVEELVDAGIPRVSARSLLRAFRRYSKMKKNDTHHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3KG65</accession>
    <name>E3KG65_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9">Lysophospholipase</fullName>
            <ecNumber evidence="2 9">3.1.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_09114</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 1-acyl-sn-glycero-3-phosphocholine + H2O = sn-glycerol 3-phosphocholine + a fatty acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15177"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16870"/>
            <dbReference type="ChEBI" id="CHEBI:28868"/>
            <dbReference type="ChEBI" id="CHEBI:58168"/>
            <dbReference type="EC" id="3.1.1.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the lysophospholipase family.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.5" evidence="2 9"/>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EFP83161.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327580.2">
        <property type="nucleotide sequence ID" value="XM_003327532.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KG65"/>
    <dbReference type="FunCoup" id="E3KG65">
        <property type="interactions" value="216"/>
    </dbReference>
    <dbReference type="GeneID" id="10544874"/>
    <dbReference type="KEGG" id="pgr:PGTG_09114"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09114"/>
    <dbReference type="eggNOG" id="KOG1325">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014602_1_0_1"/>
    <dbReference type="InParanoid" id="E3KG65"/>
    <dbReference type="OrthoDB" id="4084751at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004622">
        <property type="term" value="F:phosphatidylcholine lysophospholipase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004623">
        <property type="term" value="F:phospholipase A2 activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046475">
        <property type="term" value="P:glycerophospholipid catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1090.10:FF:000030">
        <property type="entry name" value="Lysophospholipase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1090.10">
        <property type="entry name" value="Cytosolic phospholipase A2 catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016035">
        <property type="entry name" value="Acyl_Trfase/lysoPLipase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002642">
        <property type="entry name" value="LysoPLipase_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728">
        <property type="entry name" value="CYTOSOLIC PHOSPHOLIPASE A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10728:SF33">
        <property type="entry name" value="LYSOPHOSPHOLIPASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01735">
        <property type="entry name" value="PLA2_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00022">
        <property type="entry name" value="PLAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52151">
        <property type="entry name" value="FabD/lysophospholipase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51210">
        <property type="entry name" value="PLA2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4 8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8 9" id="KW-0442">Lipid degradation</keyword>
    <keyword evidence="6 8 9" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5005127847" description="Lysophospholipase" evidence="9">
        <location>
            <begin position="25"/>
            <end position="594"/>
        </location>
    </feature>
    <feature type="domain" description="PLA2c" evidence="10">
        <location>
            <begin position="49"/>
            <end position="594"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008780"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP83161.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="594" mass="66250" checksum="6F3F66D1610CF2D9" modified="2012-04-18" version="2">MISSNLVVIVHYLLLLAQSSPSTGRHVLERRDPALTTSPSGNYAPVYDKCPARFYLREPNKLTNGRAELSDVERNYITEKAQKSINPWRKYLENVKLKDFDINSFLREAENRGGLAGDTLPNFGLSLSGGGGRALCLSGSILEAFDSRNPRADAARVGGILQLSNYAVGVSGASWLLGAWGTSNFPPISDMAPKWRLSEQNDLWDWNVVKHYRKVYKVVKQKKLAGFATNIIDVWGRLLSRLFIDDPTQEDPNQGEGVLWSSISQTPLYKDRQVPYVISVATSRPGIKEDFTPYSPIYEFSAEEFGVFHPRLNVSIPMQYLGSPQNLNGVHNTCVRGFDNAGFIMGLSSNIYSMIDSPNDHKPMVLKIVDKITDDDNFEGKVPNTFQNLGQVPEDDSPGFQDNSRDTILMADCGFINESIPIYTLLQPERKIDAIIAVDASTDGKEADPTLLRYPNGTALFSSYSRTLLPMYRGRHMPKIPSSINGSFTDLGYHRRPTFFGCNDFQGPLIIYLPNYYAVGETNQPTSKTTYTPEEIQIFYENGFAIATQNAGPTRNPDWPACLACALIDRQVLRNSASRTAECQACFQQYCAKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>E3KG89</accession>
    <name>E3KG89_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thioredoxin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_09137</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178285">
        <property type="protein sequence ID" value="EFP83184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327603.1">
        <property type="nucleotide sequence ID" value="XM_003327555.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KG89"/>
    <dbReference type="FunCoup" id="E3KG89">
        <property type="interactions" value="234"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KG89"/>
    <dbReference type="GeneID" id="10544897"/>
    <dbReference type="KEGG" id="pgr:PGTG_09137"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09137"/>
    <dbReference type="eggNOG" id="KOG0907">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090389_1_1_1"/>
    <dbReference type="InParanoid" id="E3KG89"/>
    <dbReference type="OMA" id="FQTKGRT"/>
    <dbReference type="OrthoDB" id="10263751at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017937">
        <property type="entry name" value="Thioredoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46115">
        <property type="entry name" value="THIOREDOXIN-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00421">
        <property type="entry name" value="THIOREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00194">
        <property type="entry name" value="THIOREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thioredoxin" evidence="3">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="107"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="107"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="126"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP83184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="185" mass="20140" checksum="659CB38184476614" modified="2011-01-11" version="1">MSITHISNLDQLNKLISQHKNTIIVIDFHAQWCGPCHAIADFYSSLANSNKSLTFTKCDVDQASDVAKHYQVTAMPTFVFLKAGVKIDQVRGADRANLEKLVKKYAGQSSTPSGSTFQGTGHKLGTSSSSSSTSTSTASTAARVVPNVQALNTQTLIDRWNQLSDYHKLGIILFSVYLFSIYSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHF5</accession>
    <name>E3KHF5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_09443</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328149.1">
        <property type="nucleotide sequence ID" value="XM_003328101.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHF5"/>
    <dbReference type="GeneID" id="10532417"/>
    <dbReference type="KEGG" id="pgr:PGTG_09443"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09443"/>
    <dbReference type="HOGENOM" id="CLU_1008793_0_0_1"/>
    <dbReference type="InParanoid" id="E3KHF5"/>
    <dbReference type="OrthoDB" id="10375894at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP83730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="276" mass="30697" checksum="BFA82B87468504A0" modified="2011-01-11" version="1">MQIIHGVHSLFGSARLSSVVKDVCVSPNEEEDEPSGGAVPRLARRVCGRGRSGLYILLDVSSQPATTPRVGFEKKMMIKKTVWRDGYSVQGPCLLNRNKYTNPSSSHLISVCSFPRIKPGLSSRILQAHDPWDSSRADHLHLQPRDKTSTNIIWFASSTLFPLPQKPRSTLKANLTNQATVRTVSTRAVFQAKDFIVFSRNRQSQIIYRTKTLQLAPRADSCTPSDHSNRLVSTLGPWITSQTTSSIESSIHHHPSTDSPMHRPLLRLSISIPAEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHK5</accession>
    <name>E3KHK5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribose-5-phosphate isomerase</fullName>
            <ecNumber evidence="4">5.3.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">D-ribose-5-phosphate ketol-isomerase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Phosphoriboisomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_09493</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>aldehydo-D-ribose 5-phosphate = D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14657"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="EC" id="5.3.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate degradation; pentose phosphate pathway; D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the ribose 5-phosphate isomerase family.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.6" evidence="4"/>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328199.1">
        <property type="nucleotide sequence ID" value="XM_003328151.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHK5"/>
    <dbReference type="FunCoup" id="E3KHK5">
        <property type="interactions" value="568"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KHK5"/>
    <dbReference type="GeneID" id="10532467"/>
    <dbReference type="KEGG" id="pgr:PGTG_09493"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09493"/>
    <dbReference type="eggNOG" id="KOG3075">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056590_0_0_1"/>
    <dbReference type="InParanoid" id="E3KHK5"/>
    <dbReference type="OMA" id="ACHVQEK"/>
    <dbReference type="OrthoDB" id="1555531at2759"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00412"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004751">
        <property type="term" value="F:ribose-5-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006014">
        <property type="term" value="P:D-ribose metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01398">
        <property type="entry name" value="RPI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000014">
        <property type="entry name" value="Ribose 5-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000053">
        <property type="entry name" value="Ribose-5-phosphate isomerase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004788">
        <property type="entry name" value="Ribose5P_isomerase_type_A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001924">
        <property type="entry name" value="PRK00702.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00021">
        <property type="entry name" value="rpiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934:SF0">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06026">
        <property type="entry name" value="Rib_5-P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75445">
        <property type="entry name" value="D-ribose-5-phosphate isomerase (RpiA), lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="292"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029734"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP83780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="311" mass="33716" checksum="09A0A996131FAAE0" modified="2011-01-11" version="1">MTGQFNTFSQASTAPLHSKPDPKLQTRQSVPATNPEGLDPIEASKQLAAYAAVDQHILPHHRVIGIGSGSTVPYVVERIVQQGHETNESRWFIPTGFQSKELIVKAGLNLGDVDQFPRLDITLDGADEVDADLNAIKGGGACHLREKVLAEAADKFILVADARKDSALLGTNWRAGVPIEVVPFAWAKVMSNIQLMGCPHPTLRMGKSKAGPVVTDNGNFVIDAPFDDIYMRDPSELSYRLKMLTGVLEVGLFCGMAQEAYFGNSDGTVTIRSKNGKTRKMSVEEGKLKIRSNGLTPHHLLHPLNSSASKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIE6</accession>
    <name>E3KIE6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="4">26S proteasome regulatory subunit N12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_10449</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the proteasome subunit S14 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328490.1">
        <property type="nucleotide sequence ID" value="XM_003328442.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIE6"/>
    <dbReference type="FunCoup" id="E3KIE6">
        <property type="interactions" value="614"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KIE6"/>
    <dbReference type="GeneID" id="10545167"/>
    <dbReference type="KEGG" id="pgr:PGTG_10449"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10449"/>
    <dbReference type="eggNOG" id="KOG3151">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046003_2_1_1"/>
    <dbReference type="InParanoid" id="E3KIE6"/>
    <dbReference type="OMA" id="HIMDGYF"/>
    <dbReference type="OrthoDB" id="8775810at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.990:FF:000001">
        <property type="entry name" value="26S proteasome non-ATPase regulatory subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.990">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006746">
        <property type="entry name" value="26S_Psome_Rpn12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033464">
        <property type="entry name" value="CSN8_PSD8_EIF3K"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000717">
        <property type="entry name" value="PCI_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12387">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12387:SF0">
        <property type="entry name" value="26S PROTEASOME NON-ATPASE REGULATORY SUBUNIT 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10075">
        <property type="entry name" value="CSN8_PSD8_EIF3K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50250">
        <property type="entry name" value="PCI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PCI" evidence="3">
        <location>
            <begin position="84"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP84071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="279" mass="31794" checksum="AE7735EF6008594D" modified="2011-01-11" version="1">MSTMKLSPDAERMYTQLQQQFRAPGAKDPTIAQLLLKLKLALAQSSLLMPSLTDKNAIDAESLAKARDILEIGAFYSVRTQDIPAFERYISQLGSYYNDYSSLLPSSQRRYPLVGLDLLRLLSQNKIAQFHTVLEGLVKEHGQKNIDILHTNQYISHPVNLERWLMEGSYSKVWGARSQMPMEEYRYFVDLLVDTIRNEIASCQEKAYSSLPLNDAGTLLFFKTPGEVIEFAQQRKWLVDPKSQTINFPAENNATKHELAKEKIIETTLGYAKELETIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIE7</accession>
    <name>E3KIE7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Proteasome subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_10450</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Component of the proteasome, a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the proteasome complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the peptidase T1B family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328491.1">
        <property type="nucleotide sequence ID" value="XM_003328443.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIE7"/>
    <dbReference type="FunCoup" id="E3KIE7">
        <property type="interactions" value="429"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KIE7"/>
    <dbReference type="MEROPS" id="T01.010"/>
    <dbReference type="GeneID" id="10545168"/>
    <dbReference type="KEGG" id="pgr:PGTG_10450"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10450"/>
    <dbReference type="eggNOG" id="KOG0174">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035750_5_2_1"/>
    <dbReference type="InParanoid" id="E3KIE7"/>
    <dbReference type="OMA" id="TFIYGYC"/>
    <dbReference type="OrthoDB" id="7854943at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004175">
        <property type="term" value="F:endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03762">
        <property type="entry name" value="proteasome_beta_type_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000010">
        <property type="entry name" value="Proteasome subunit beta type-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF0">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE SUBUNIT HSLV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="6 10 11" id="KW-0647">Proteasome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0888">Threonine protease</keyword>
    <keyword evidence="7" id="KW-0865">Zymogen</keyword>
    <feature type="active site" description="Nucleophile" evidence="9">
        <location>
            <position position="19"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP84072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="219" mass="23684" checksum="8AE9D613C67B8F6D" modified="2011-01-11" version="1">MEHDLDINRLKSGELNLGTSIMAVAFEGGVVIGADSRTTSGSYIANRVTDKLTHIHDRVYCCRSGSAADTQAVADYVHLYCQMYTAQHGHAPSVHTVGTIFEQICYSNKDNLSAGIIVAGWDAQTKKGSVYNIPLGGGLFQQPWAIGGSGSTYIYGYCDSNYRAGWTKEETVGFVKNALALAMSRDGSSGGTIRMAIITEDKVERVFIPGNQLPVFWEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIS7</accession>
    <name>E3KIS7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328621.1">
        <property type="nucleotide sequence ID" value="XM_003328573.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIS7"/>
    <dbReference type="GeneID" id="10545462"/>
    <dbReference type="KEGG" id="pgr:PGTG_10580"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10580"/>
    <dbReference type="HOGENOM" id="CLU_1050252_0_0_1"/>
    <dbReference type="InParanoid" id="E3KIS7"/>
    <dbReference type="OMA" id="ENQEWII"/>
    <dbReference type="OrthoDB" id="2498861at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="150"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="173"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="226"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="265" mass="30044" checksum="E87C3A3D282F7076" modified="2011-01-11" version="1">MSVDGFPSIMIPLEQQRRANPSVQSIWPRKQASSPETKLLNFQTAELHILLEAICALNPSLQVSRTDYTSPINFHSRRHDFRVDNACGPSPNQMYLRRYNSVTQHRELCSSDPHTLSMTRARLPGSGFGHSWEPSPTAIRCDNRQIKSISSLQSHTSPPSGTNPSNSPSQSSADANLFNNETSSGPKGHVPLKQHLTYWPKKSRKKKTWLTQKKTSKTLDTIPENQEWKMQERYPQDIMTSVLPENSEPRSVFENDSDSEDEIED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIV2</accession>
    <name>E3KIV2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_10605</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84227.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328646.2">
        <property type="nucleotide sequence ID" value="XM_003328598.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIV2"/>
    <dbReference type="GeneID" id="10545487"/>
    <dbReference type="KEGG" id="pgr:PGTG_10605"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10605"/>
    <dbReference type="eggNOG" id="KOG2533">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_0_3_1"/>
    <dbReference type="InParanoid" id="E3KIV2"/>
    <dbReference type="OrthoDB" id="2502798at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:000013">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF85">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G00710)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="120"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="146"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="212"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="251"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP84227.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="297" mass="32079" checksum="442CBE4CA6D47811" modified="2012-04-18" version="2">MVDGDTLAGAGFFCFLVSRSENICAPSLASSPATAKYPNKSTVSQVWEACKSPHVIILNIAQFSCASNTYSLAYFTPTVVHTFGYSPTDTQLFTVPPFALGVVFLLGLSYCSDRYQARGLMSGISAILSIVGFAMFYGSGDDKVRYASLFLSIPGAYGVAPCLSAWTADNSAPHARKATALAFGTMVGNSGGLFSVWIFTLGRKPRYHLPTAISLAFGVMIIMCCVMNTLWLSYAQRAKVTKRSQILERYTFPPADGGGEADPGKEGLQSRASEQELLSAQAWDHLGDQHPDFKYVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJ86</accession>
    <name>E3KJ86_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_10081</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178290">
        <property type="protein sequence ID" value="EFP84361.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328780.2">
        <property type="nucleotide sequence ID" value="XM_003328732.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJ86"/>
    <dbReference type="GeneID" id="10545788"/>
    <dbReference type="KEGG" id="pgr:PGTG_10081"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10081"/>
    <dbReference type="HOGENOM" id="CLU_2251400_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJ86"/>
    <dbReference type="OrthoDB" id="2514451at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP84361.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="104" mass="11038" checksum="23EA4880C113E3DF" modified="2012-04-18" version="2">MTTSSDMCTGVPDAILLGSSHKGKKRLLSVCPGAQDQEEDSAHQPLPLKKKRLDLNLHLGLPGVEENVQANPASSSLLAIESTDGASLEGDQTDFKISQIKQVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3KK80</accession>
    <name>E3KK80_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_10864</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84705.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329124.2">
        <property type="nucleotide sequence ID" value="XM_003329076.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KK80"/>
    <dbReference type="GeneID" id="10526672"/>
    <dbReference type="KEGG" id="pgr:PGTG_10864"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10864"/>
    <dbReference type="HOGENOM" id="CLU_2777166_0_0_1"/>
    <dbReference type="InParanoid" id="E3KK80"/>
    <dbReference type="OrthoDB" id="2508601at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP84705.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="69" mass="7975" checksum="7B71A35A75B07A21" modified="2012-04-18" version="2">MLTKLRLMVKRQNRLGRLHRQPITARSIPGNATAKKLFYLKEQPPAAARVFIKPGYQLDQDFTPPYLIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KKF0</accession>
    <name>E3KKF0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10246</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    </evidence>
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        <source>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
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                <person name="Pangilinan J.L."/>
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                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
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                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
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                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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    </evidence>
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            <fullName evidence="2">Uncharacterized protein</fullName>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
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                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
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                <person name="Morin E."/>
                <person name="Murat C."/>
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                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
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                <person name="Schmutz J."/>
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                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
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                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
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                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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            <dbReference type="PubMed" id="21536894"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
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    </reference>
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            <begin position="615"/>
            <end position="636"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="659"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="707"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="918"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1054"/>
            <end position="1071"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1136"/>
            <end position="1153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1243"/>
            <end position="1254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1274"/>
            <end position="1285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84938.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1285" mass="143545" checksum="30CB7522134E537E" modified="2012-04-18" version="2">MVQQQQQQQQHLSLQDNSNRAGPSNNITTPDKKPSKHQLKLIQHPHQQEPLKHQLSWPGPGTPTIVLDQVLLPLSPTEDPLILKVSPRHPNQYYSASPSYRHQTANQSQPPPRRSIIIPSTATRTSDSENQHNPLLDLSRTTEHPPPRTRNRSIVSPATPPKPRIWDDDNAFHAFQVEHSKLGVDLTISCDESPAETTDRRQNKNKERKKHKISMGHVEIVMSELDKARFKAQHNHNRLQVRYSTDGLRNMEIEESRRTGSPSSTPSHPDGNLSHQDSQRSSPSTGPHQLSPVHEPNHRENASLNSGSVSSLEHSRSRSRSPVFRHQSHLSISSSSGKEEANSSSTNEREQQPNRSSSEAHEQEDTSPYIPAENRLSSSPRSEEASLPEVNETEHEPRGSSYEAAAEPKDASNYRPSESRRETPSPLHDRRPYAENDTLKPSSAMSRNALSDIPSRRLTSRKSPGGFIRESEEYQPDASPINQDAFHIDQETSHTSQKESPVSENTMGRVHEPELARDRMSPESPVPQVSGMPEQVVGSDSDQEGRSNDEIASRLCSVESGHDNLPGHQRDYSEEEEEEDRQGSTTEEDSARSPSPTHPSSPVIDIIDENSQANPIPISSSVSPSVDLPSCSPQSPHHQHSLKSASLAPSDRSGLQLDQSEKEVDDRSQDPESSRRDSQHDESHSSGGSDRQSEQSPDEDPGRSPSPEIQHSSQPNEGSASESPVRDSSEVLQESQDVDHSVSLDDLTLFKAQGNLKHFTSTPRGVLHHPVPFPAVTNPTTGRAPSSTPRPSTYPIVEIASSDPKVAARAAAILKVHHGFIPRGCKVENTGLMGDGDLEREMELSIQREEADRSLRLQASSTTASSAPHNLLRTVQTKKMFDQCRPEPSQDSFHHLFTENERQRTPELNHHSRAGPRSPSSVASTTRQDSLRRKAHHNHCKSLKEELQQLDYRPDFHSWSQADWKILEFCFKRIERKQPAGPSSQPNIQPEEVVLKMLEALDLAPSDCQSEWEWTKMIYRVIALQKRRSADPSRRSRCSSATTSISQRRPAERPSSSHISSLHQTRTSGFASQALGGGPARSSSVSEEDDEEEVRREDGREVYPSLEPVRRAFSRSRTTTPLPRRAHDRHEEEVQHQGSQSSPATAPSGQQTRIPKAPPISDRFRRQSLRNTLAYWKARGPRRVGKGKIWTIVAAFEARFDKLLASLSPPPNILHYLRQTDTEVSSRPNKRALNHSDNVINPSSSSTTTTSTTTLFHPSFANDFYSEHPAPPPHKSHSWKKQRKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KL81</accession>
    <name>E3KL81_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_11225</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP85056.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329475.2">
        <property type="nucleotide sequence ID" value="XM_003329427.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KL81"/>
    <dbReference type="GeneID" id="10527033"/>
    <dbReference type="KEGG" id="pgr:PGTG_11225"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11225"/>
    <dbReference type="HOGENOM" id="CLU_1403084_0_0_1"/>
    <dbReference type="InParanoid" id="E3KL81"/>
    <dbReference type="OrthoDB" id="10268946at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP85056.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="223" mass="26003" checksum="DF1DB3C9B3070758" modified="2012-04-18" version="2">MLEEHLEEWYRGPVSLPEPDTTEFKQFERLQGDLAIKRFELLSAHLFHWPSTITRDAPTDISIDRLRSKKDIHTHFHSSLLPLLKQHFNSVAEVLGDLTELRKDPAPRIQRVLEIQPYLHRDLDQMIRIIDDIIPGKVPEPSQTNDQHFEHFKSYRLFGLDNCIRGELRCRIAGFFYDSCIIIEELKLPARKRFPSSLGSQHSLNAAFDTTLKWLNEDPGNPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KMX8</accession>
    <name>E3KMX8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10943</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EFP85614.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330033.2">
        <property type="nucleotide sequence ID" value="XM_003329985.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KMX8"/>
    <dbReference type="GeneID" id="10544179"/>
    <dbReference type="KEGG" id="pgr:PGTG_10943"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10943"/>
    <dbReference type="HOGENOM" id="CLU_2237925_0_0_1"/>
    <dbReference type="InParanoid" id="E3KMX8"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="68"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85614.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="105" mass="11220" checksum="2EAE7EDA0826B92C" modified="2012-04-18" version="2">MPRTLGTPLDHSDQASERSENPQHPSKPPHNPRLLSDSPDQLGSSAAKHPSKALEPQRPPPARPKAPKQPARAPQSPQGAPQSAPQPHQGPRIPKKSGVDDIQTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNS9</accession>
    <name>E3KNS9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11710</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178297">
        <property type="protein sequence ID" value="EFP85954.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330373.2">
        <property type="nucleotide sequence ID" value="XM_003330325.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNS9"/>
    <dbReference type="GeneID" id="10539953"/>
    <dbReference type="KEGG" id="pgr:PGTG_11710"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11710"/>
    <dbReference type="HOGENOM" id="CLU_033568_0_0_1"/>
    <dbReference type="InParanoid" id="E3KNS9"/>
    <dbReference type="OrthoDB" id="2501717at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173580" evidence="1">
        <location>
            <begin position="28"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85954.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="442" mass="50294" checksum="72386C4F55A2503D" modified="2012-04-18" version="2">MENLLGYALDMLFQLSLFALLDTSTCSIAFNELASHASPGLHGDSIYKTGNQGRIEGPCVKEAKLNLHPSNIQQACTQGSGKKFIITEEDRKAIEIGEGKTSPTGDSPKVHNSEYSKNDIEWEPNLKFQPKNSLMEGGLRIKTEDNVRVTQNLFESVTKSEAERGLAMEEDFRIRIKASFQPRESKDTSEIFEGVIKPQWRHFIDSKSQASVFLEESVQSLQLSGKNGNKIKQRDPQKDVKFTPAIMEPFGEMFQLDREFPKVLDAPQMHPMAPHFKAWMSDLESNRRFRDPMDIGPRPTIKLSSDVSEILEEEERLNGADPWNVYHWINCLGLGQVENLEDLNDLDISTSVDIILALLHSPNYKIIPWYESPDRACVIRMFTEEKYYQHLNYICNRLQKMSGGSGSKGNDWKQEAPVSEILKYQAQDKVMIYDHGLDGKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPX4</accession>
    <name>E3KPX4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Trafficking protein particle complex subunit 13 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_12315</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178299">
        <property type="protein sequence ID" value="EFP86359.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330778.2">
        <property type="nucleotide sequence ID" value="XM_003330730.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KPX4"/>
    <dbReference type="STRING" id="418459.E3KPX4"/>
    <dbReference type="GeneID" id="10540373"/>
    <dbReference type="KEGG" id="pgr:PGTG_12315"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12315"/>
    <dbReference type="eggNOG" id="KOG2625">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_679953_0_0_1"/>
    <dbReference type="InParanoid" id="E3KPX4"/>
    <dbReference type="OrthoDB" id="10250284at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990072">
        <property type="term" value="C:TRAPPIII protein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010378">
        <property type="entry name" value="TRAPPC13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055427">
        <property type="entry name" value="TRAPPC13_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13134">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13134:SF3">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06159">
        <property type="entry name" value="TRAPPC13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Trafficking protein particle complex subunit 13 N-terminal" evidence="2">
        <location>
            <begin position="23"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="129"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="264"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="324"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="290"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="344"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="396"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF06159"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP86359.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="405" mass="45109" checksum="6576D19C1937DD29" modified="2012-04-18" version="2">MNNNRAHLLSLKVLRSSPPTLNTTTNQPTQTEIADTLSLPNSFGTIYQGEAFNGLLSLRPEQPRSNLIAALNPKLIVELQSSQSLHKSLIGSIHAHQLGPASEHEALELLINHQITQLGLHSLICTVTYQEPPPTEPTEEEEDQELTPAESHQITPESEPQTRSFRKLYKFQVLNPLGIKTKTYRSPSSSSVLEETRVLESLKKVLAEIEAHDHHHYDDTALSSLFDGQDRLGNDGSSSNETCYMEIQIQNQSSKPLLNLTLNLLPQDQNPDDQKEEEGESTTRKIEQIQLKQRPTRTPTGAESDEQIMPGDIRQYAFKVDKLDEFDGRAGAGETADRSRRADDDDDADADETDDDDDDDDGDRELGLSDGRCAEPVLQGRAAPGRPADRRRRALPSRTPNLIFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3KR84</accession>
    <name>E3KR84_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13191</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178302">
        <property type="protein sequence ID" value="EFP86809.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331228.2">
        <property type="nucleotide sequence ID" value="XM_003331180.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KR84"/>
    <dbReference type="GeneID" id="10541396"/>
    <dbReference type="KEGG" id="pgr:PGTG_13191"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13191"/>
    <dbReference type="HOGENOM" id="CLU_2110140_0_0_1"/>
    <dbReference type="InParanoid" id="E3KR84"/>
    <dbReference type="OrthoDB" id="2506774at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86809.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="136" mass="14536" checksum="66F7DDA14BA0456B" modified="2012-04-18" version="2">MASQTTAEIQMNHDRLDTDNRMVDANIALINALSQGLAPTANSPEQISLQNHQIRLDVQLREVEVARARAALAAEEASGAAFARARMVQDFIGSGLAPAEALEMTNQILGPVLLAPPQTPVDPSQTPVDPSPPYDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3KS69</accession>
    <name>E3KS69_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Wax synthase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_13363</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Secondary metabolite biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the wax synthase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EFP87144.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331563.2">
        <property type="nucleotide sequence ID" value="XM_003331515.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KS69"/>
    <dbReference type="STRING" id="418459.E3KS69"/>
    <dbReference type="GeneID" id="10542387"/>
    <dbReference type="KEGG" id="pgr:PGTG_13363"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13363"/>
    <dbReference type="HOGENOM" id="CLU_057743_0_0_1"/>
    <dbReference type="InParanoid" id="E3KS69"/>
    <dbReference type="OrthoDB" id="1077582at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008374">
        <property type="term" value="F:O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044851">
        <property type="entry name" value="Wax_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032805">
        <property type="entry name" value="Wax_synthase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31595">
        <property type="entry name" value="LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31595:SF57">
        <property type="entry name" value="OS04G0481900 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13813">
        <property type="entry name" value="MBOAT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="279"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="312"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="370"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="domain" description="Wax synthase" evidence="9">
        <location>
            <begin position="257"/>
            <end position="347"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF13813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP87144.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="429" mass="48219" checksum="5BF6AD163CA39944" modified="2012-04-18" version="2">MNGLGVFSHLVPLTVQASLFNLVWTRDDYFQKPLIRWTRISLLPITLGVMFSELVNLKYQAELSSHTKLCIGSVITSQAMKSVMFTFKQPVSSSPIKTKDYPAPQPPSFLSTLTNVISLVLASSSDPSKREISGSGTNHRIHSDIMFLSKTFRRLLVHHICAVIALACWRGVNDEALIDQLAVGPMIKKFKCEITAFCCGNLIWTNMDLFGCLLRLFWFTVKIINRLLLKLLPSVFKNMPPSEKLNQTDLEETSPLLFKKTPLEATSMTDFWGRHWHQMIRSLVVEAGAVPVTSFLVWVFGTDQLHPKVLRLAGVLAAFTISGLVHEAGVWTAGPIDLTFKTTIFFISQGVAICLENVFKQAFGRKVDGFLGRIWTFSWLVYFGTPMVTLWLNTAFFDQTGLFQKVDELGLLRMSLMPLIVPKLLSHSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KSQ8</accession>
    <name>E3KSQ8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_12775</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178305">
        <property type="protein sequence ID" value="EFP87191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331610.1">
        <property type="nucleotide sequence ID" value="XM_003331562.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KSQ8"/>
    <dbReference type="GeneID" id="10537912"/>
    <dbReference type="KEGG" id="pgr:PGTG_12775"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12775"/>
    <dbReference type="HOGENOM" id="CLU_2005023_0_0_1"/>
    <dbReference type="InParanoid" id="E3KSQ8"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP87191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="124" mass="13704" checksum="7B6E7C0C8B6D9096" modified="2011-01-11" version="1">MSVGMGSPAHILSIKKNQNTMILTSQANIVTSCQQVLKVIDLSNLISLFKRCTESEWYQVTEFGLSELTKKVQDTLDACRPKSDRHPTKSLSVRIVYQSFTVCWKGTSKELNSSSGDISSTGVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>E3KTP0</accession>
    <name>E3KTP0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_13527</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178308">
        <property type="protein sequence ID" value="EFP87741.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332160.2">
        <property type="nucleotide sequence ID" value="XM_003332112.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KTP0"/>
    <dbReference type="GeneID" id="10539139"/>
    <dbReference type="KEGG" id="pgr:PGTG_13527"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13527"/>
    <dbReference type="HOGENOM" id="CLU_1769042_0_0_1"/>
    <dbReference type="InParanoid" id="E3KTP0"/>
    <dbReference type="OrthoDB" id="8062037at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45969:SF69">
        <property type="entry name" value="FINGER DOMAIN PROTEIN, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G12190)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45969">
        <property type="entry name" value="RING ZINC FINGER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 4" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="6">
        <location>
            <begin position="57"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="120"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP87741.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="147" mass="15925" checksum="6745634A6602B5A8" modified="2012-04-18" version="2">MKQNVVTFHEIYNGNYVAGSRPVSDPLASVGPNFLLKSSNLHAKRGLSETPSSSTICAICLDSLIEEEAFEWTGCAHRFHDYCVESLSWVGGALEAPTCPLCRAPDGSLGERLRRLRAGTARPAQHFSSLRPGPRLGLPSNGQESWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KUZ9</accession>
    <name>E3KUZ9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12546</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178311">
        <property type="protein sequence ID" value="EFP88099.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332518.2">
        <property type="nucleotide sequence ID" value="XM_003332470.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KUZ9"/>
    <dbReference type="STRING" id="418459.E3KUZ9"/>
    <dbReference type="GeneID" id="10535241"/>
    <dbReference type="KEGG" id="pgr:PGTG_12546"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12546"/>
    <dbReference type="HOGENOM" id="CLU_041048_0_0_1"/>
    <dbReference type="InParanoid" id="E3KUZ9"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="54"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="154"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88099.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="471" mass="52260" checksum="F45E0E3A4B78B09E" modified="2012-04-18" version="2">MATLKAVCAVPPGFKFPGPWICLSTYQATGTTSYLTNTNSDTFSSSNRIDRQILGKKPRNSSRHPNTVAEAGLSKNASGPQFCFWPVKVVSTIRKEKTTGNRLVQTRLPVGLGFVRSKGPGKMRTPAGIPLIGLLPTPYSRIKLRDMRPLPPSTNPSPGTYHSYPMSTPPTTNTGVGMNATEPIDPRFQPPPYNLNLPRFPHPVFYPHPPMFHPPGQAFPPFGQGGGPRGRGFVGGRGRGRGGGRGRGWLPPAQLRIRGQANRFYYPNGPAAGVPFGHGHAPLFNSNRAQVDMDNFLNQHPIPPAVQRRPTPIPVPSLPSVPAANVARAPSNLSSIRSGNSFYTAEDFLAPQVWVASPTPAGMYNPDDYHLLARHQIESLLDRCAAPKRFDRYEFTPQHFDNFYQSATSALFHHWTRHPAAKANERQDRVRLFQFITRQYRTMTRRVVPLRWNRLCCLSIMDSQSIGVKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVD3</accession>
    <name>E3KVD3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14312</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178312">
        <property type="protein sequence ID" value="EFP88228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332647.1">
        <property type="nucleotide sequence ID" value="XM_003332599.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KVD3"/>
    <dbReference type="STRING" id="418459.E3KVD3"/>
    <dbReference type="GeneID" id="10535538"/>
    <dbReference type="KEGG" id="pgr:PGTG_14312"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14312"/>
    <dbReference type="eggNOG" id="ENOG502SAQ9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_370119_0_0_1"/>
    <dbReference type="InParanoid" id="E3KVD3"/>
    <dbReference type="OMA" id="DETVNCC"/>
    <dbReference type="OrthoDB" id="2507795at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018822">
        <property type="entry name" value="UPF0646"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10336">
        <property type="entry name" value="DUF2420"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="178"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="220"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="416"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="499"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="627"/>
            <end position="711"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="296"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="317"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="376"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="515"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="630"/>
            <end position="640"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="648"/>
            <end position="663"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="690"/>
            <end position="700"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="752" mass="83017" checksum="9B664AC0C0C60DFB" modified="2011-01-11" version="1">MMDEWDLQQDNTNIQPRLNTELLERIPPAPPGGIRLICEGCEYSLFQAIQPIQEDQPKIEEQTILLSEPDYQELYYGPLSNFISQGLHQIFPNLHDQHKNEFILHFTNLEIKVPEDNIYSKELSLFDIDRLHIGARLPDRLMISLEKQPRLVHRFNILANYVAELEPILSQDELSAVELDHHQSSGPSTHEPEEIKEPIDQPSTNPETIGDTEAIVDSEVQEAPEDAHEAPVDSELQEEPIVDSELQEAPLESELQEAPECSEVQETPVDSELQEAHEGSEANEISEAEPELLGNQAPQEQSSELVPIDNQNEQHSATTTTTTATVSLPIDPSDSPSSSLLLGAPPSSAKEDHQLELESAPGTDHLESGLTATATADDDYDENDALERVEEEEEEEEEVDVEREQGGCDLEAIDDGHLSGGELEVTSLVVDHLVGGELEEIENEVEVDVAAGANDDDDGVVVDDQLELDELEGDGEIVGLEPASHDQLIGAESKVRVNDQANLAGSEVEESNGDEDQHLEPHPDHSAPLVPTIDLEKNPLESQASFNMDLVAPSSVDHQELLISSNDPNVLYNEVEHEEVEHEEALEMIEQEQIVLEPNLDLDYDPGTESIELLVDQSVGYPEFFEQVDGSGSSSDQTVNGFEHLSEDQVDEPVEGFQDDEQTDQLKEPDSDTVEASYPATHHTLHPSDSPETNLVSSLPPSDHIFPPLIGSKRSHAQVDGLFVDDPSCRSVIESSSSHESLFLDVKKPRTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KW39</accession>
    <name>E3KW39_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14092</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178314">
        <property type="protein sequence ID" value="EFP88514.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332933.2">
        <property type="nucleotide sequence ID" value="XM_003332885.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KW39"/>
    <dbReference type="GeneID" id="10540978"/>
    <dbReference type="KEGG" id="pgr:PGTG_14092"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14092"/>
    <dbReference type="HOGENOM" id="CLU_2574992_0_0_1"/>
    <dbReference type="InParanoid" id="E3KW39"/>
    <dbReference type="OrthoDB" id="2508611at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="23"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88514.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="81" mass="8888" checksum="370E77FF4C88E3E7" modified="2012-04-18" version="2">MKKRLLDTLQRSFPDGGVDPSANEPSSTADSLAMGSNVSSTRPAADWLRMSSARRTTGLQIRCRKISEQQGRAIARLVRLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3KWS5</accession>
    <name>E3KWS5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Protein-serine/threonine kinase</fullName>
            <ecNumber evidence="7">2.7.11.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_14708</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Mitochondrion matrix</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the PDK/BCKDK protein kinase family.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.-" evidence="7"/>
    <dbReference type="EMBL" id="DS178316">
        <property type="protein sequence ID" value="EFP88742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333161.1">
        <property type="nucleotide sequence ID" value="XM_003333113.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KWS5"/>
    <dbReference type="STRING" id="418459.E3KWS5"/>
    <dbReference type="GeneID" id="10541693"/>
    <dbReference type="KEGG" id="pgr:PGTG_14708"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14708"/>
    <dbReference type="eggNOG" id="KOG0787">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023861_0_1_1"/>
    <dbReference type="InParanoid" id="E3KWS5"/>
    <dbReference type="OMA" id="AAYYPPE"/>
    <dbReference type="OrthoDB" id="3264224at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004740">
        <property type="term" value="F:pyruvate dehydrogenase (acetyl-transferring) kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010906">
        <property type="term" value="P:regulation of glucose metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010510">
        <property type="term" value="P:regulation of pyruvate decarboxylation to acetyl-CoA"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.20">
        <property type="entry name" value="Alpha-ketoacid/pyruvate dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036784">
        <property type="entry name" value="AK/P_DHK_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018955">
        <property type="entry name" value="BCDHK/PDK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039028">
        <property type="entry name" value="BCKD/PDK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947:SF25">
        <property type="entry name" value="[PYRUVATE DEHYDROGENASE (ACETYL-TRANSFERRING)] KINASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11947">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10436">
        <property type="entry name" value="BCDHK_Adom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69012">
        <property type="entry name" value="alpha-ketoacid dehydrogenase kinase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Branched-chain alpha-ketoacid dehydrogenase kinase/Pyruvate dehydrogenase kinase N-terminal" evidence="9">
        <location>
            <begin position="53"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="388"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="398"/>
            <end position="408"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF10436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP88742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="465" mass="52284" checksum="9DD0FCA87B1C1F0E" modified="2011-01-11" version="1">MNRIRPSLKSSAWRSSSSASRQSASPLGNEDNQKINFYSNSHILRYAEMEAAPFSLRQLIFFGKVLGRQGSNDKEVDHNLMQGANFTRVQLAIRLARQIREFQSLPYIVTSNPYLTETYQMYVEAFESFRLYPEIRCRLDNQKWSQFLENLLNQHKVVIPKLAIGVAESRSHLTNSQVEQFMTRMLYSRISRRVLAEHHIALTRQFQESSSSKPSSQNIRYLGVIDTELEIGNVVQRCIELAQTTLNSHSLGGSSSQQPVTVLVRGEKDAKFAYIPDHLEFIVFELLLNAFRATISSAVQRSLDLASLPIEVQIASSATHITIRISDQAGGILHSWRPSFTDDDDESLPPTVSRREMFSFVNMTTANDSLKQLVSSTDGTMEGKVREQVDQAHDQPHIPSSSISSPSSTNTRGLEQIQEARLRIGLPLSSIYAQFLGGSLEMYSIPGFSDIVLCIPKLGTTTEPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3KX55</accession>
    <name>E3KX55_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_14164</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178317">
        <property type="protein sequence ID" value="EFP88825.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333244.2">
        <property type="nucleotide sequence ID" value="XM_003333196.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KX55"/>
    <dbReference type="GeneID" id="10541940"/>
    <dbReference type="KEGG" id="pgr:PGTG_14164"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14164"/>
    <dbReference type="HOGENOM" id="CLU_683592_0_0_1"/>
    <dbReference type="InParanoid" id="E3KX55"/>
    <dbReference type="OrthoDB" id="2499895at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="206"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="336"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="354"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP88825.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="403" mass="44468" checksum="9FC624F0770C88A9" modified="2012-04-18" version="2">MNFPFVTLLIYTININLKYTNARAILPRDYSSSSPLSPASYDKEDGMNENLNRLNLGPTFSVRPSKDAENLDSIPSLDADKEQQSQRKSIVLSSSLNFSIRVEDASKDHNTILIPIVLSLQNHEPDASATPIISNSELKPSIIPGDEEYASRNDSSLMISHSSGRDYFLVSLLSPNISDEGTHAHPTNWTVLIIARHISNPETLNWTWILVGIIALICILLAAICPIILAYCDYFGGGRDDEENMVVDLITPFRHQDYPSEIQKEDTDFRSLGPGRATKADMSKPLKLNRGPTAGEFKNTKNKENCFAEIDHDGDGISLNDFKICQARKMINLQPFRPSGHQYNDKNSSDSLGSPTATSTSSFTANSPVSPLTTITSPKSFQSLAFSDYHSPLPSLPKKCVTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3KX94</accession>
    <name>E3KX94_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_15170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178318">
        <property type="protein sequence ID" value="EFP88967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333386.1">
        <property type="nucleotide sequence ID" value="XM_003333338.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KX94"/>
    <dbReference type="GeneID" id="10542408"/>
    <dbReference type="KEGG" id="pgr:PGTG_15170"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15170"/>
    <dbReference type="HOGENOM" id="CLU_2211272_0_0_1"/>
    <dbReference type="InParanoid" id="E3KX94"/>
    <dbReference type="OrthoDB" id="10354505at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP88967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="107" mass="11493" checksum="1CDE74ADB29CC1DE" modified="2011-01-11" version="1">MTAAVSNARHADYFVSACSCSRFVGLSFLGTHDESTLSNLQTGAKSCRPRDNVNGKRTTELCDTQYTDGYCGTSHGSFICNGGYYKDGPHANQPQGCQDCDDLEPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>E3KXZ7</accession>
    <name>E3KXZ7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ATP-dependent DNA helicase</fullName>
            <ecNumber evidence="11">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGTG_15039</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="11"/>
    <dbReference type="EMBL" id="DS178320">
        <property type="protein sequence ID" value="EFP89198.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333617.2">
        <property type="nucleotide sequence ID" value="XM_003333569.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KXZ7"/>
    <dbReference type="STRING" id="418459.E3KXZ7"/>
    <dbReference type="GeneID" id="10546835"/>
    <dbReference type="KEGG" id="pgr:PGTG_15039"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15039"/>
    <dbReference type="eggNOG" id="KOG0353">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001103_12_5_1"/>
    <dbReference type="InParanoid" id="E3KXZ7"/>
    <dbReference type="OrthoDB" id="10261556at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18794">
        <property type="entry name" value="SF2_C_RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:001219">
        <property type="entry name" value="ATP-dependent DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001544">
        <property type="entry name" value="ATP-dependent DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001975">
        <property type="entry name" value="ATP-dependent DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002464">
        <property type="entry name" value="DNA/RNA_helicase_DEAH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004589">
        <property type="entry name" value="DNA_helicase_ATP-dep_RecQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032284">
        <property type="entry name" value="RecQ_Zn-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00614">
        <property type="entry name" value="recQ_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16124">
        <property type="entry name" value="RecQ_Zn_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00690">
        <property type="entry name" value="DEAH_ATP_HELICASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 11" id="KW-0347">Helicase</keyword>
    <keyword evidence="4 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="13">
        <location>
            <begin position="116"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="14">
        <location>
            <begin position="350"/>
            <end position="504"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="690"/>
            <end position="796"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="690"/>
            <end position="710"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="711"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="12">
        <location>
            <begin position="746"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="12">
        <location>
            <begin position="771"/>
            <end position="785"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EFP89198.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="796" mass="87134" checksum="60A489858FB23E5D" modified="2012-04-18" version="2">MSSEEVEAIQEELAIVRASIESLQETEKALASRLQTLLDGGTGHEDAGNYVLSDDLEVPQEKSRRLNKQLIDFTSAFQPSSSDQEKLPWSWTQALHETMKKIWGIESFRLNQEAVCNAVLSNRDVLVIMPTGGGKSLTYQLPAVLSEGTSLVISPLVALMADQNMHLKEAGVPAEMLNASTSRQDAALIMKRLLHSVVDQKPKRGSKSSKGKEKAHEDDLAPIKLCYVTPEKIAKSKTFVSTLQKMYGAGRLSRVIIDEAHCCSSMGHDFRPDYKQLSILKTLFPDTPIVALTATCPPRVMVDVLKILKLGPITNADKARPTGTVLFTSPLSRPNLHYQVLKKPATNTDLIDQIVEWIEQNHSGSQGIIYTLSQKDTTTVAQGLISQSNGRITTGIYHASLSDSHKHQVHTDWREGSIQVVCATTAFGMGIDAPHVRFVIHHTLPKSMEGYYQESGRAGRDGQVSDCLLFWKTSDLLRLSGMVASEVDGIPKLYSMTKYATELERCRSLLFAEYFNTSHSGADSVLPFSQEACGNCDNCKRDPSEIKQEDLSVEALKICDVVGYVNKMGGRVTLVQLGDLVRGIGTPMSTIISNSQARKDVDDFLADVPAGIPVPDLSEGKVLLKKEQVENLILALLLRGYLQEQFVATAYTVNSYIKLGPKSRVLLTSRDAISSGKALPSNVKVLIASSSSTTARTARTNRKTSTTGAKPRAKKAAPKKRKVADDSDSSIECSKGKRAAKVVVSDGDDNIYNDEDGESGLDEDQPILSNRIRKHPSNQGPHKKITMIEIDSGDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="68" xmlns="https://uniprot.org/uniprot">
    <accession>E3L081</accession>
    <name>E3L081_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">beta-galactosidase</fullName>
            <ecNumber evidence="3">3.2.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_16256</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.</text>
            <dbReference type="EC" id="3.2.1.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the glycosyl hydrolase 35 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.23" evidence="3"/>
    <dbReference type="EMBL" id="DS178327">
        <property type="protein sequence ID" value="EFP89968.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334387.2">
        <property type="nucleotide sequence ID" value="XM_003334339.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L081"/>
    <dbReference type="STRING" id="418459.E3L081"/>
    <dbReference type="GeneID" id="10545863"/>
    <dbReference type="KEGG" id="pgr:PGTG_16256"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16256"/>
    <dbReference type="HOGENOM" id="CLU_005732_2_0_1"/>
    <dbReference type="InParanoid" id="E3L081"/>
    <dbReference type="OrthoDB" id="1657402at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005773">
        <property type="term" value="C:vacuole"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004565">
        <property type="term" value="F:beta-galactosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019388">
        <property type="term" value="P:galactose catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.260:FF:000302">
        <property type="entry name" value="Beta-galactosidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.102.20.10">
        <property type="entry name" value="Beta-galactosidase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.390.10">
        <property type="entry name" value="Beta-galactosidase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018954">
        <property type="entry name" value="Betagal_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037110">
        <property type="entry name" value="Betagal_dom2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025972">
        <property type="entry name" value="BetaGal_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036833">
        <property type="entry name" value="BetaGal_dom3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025300">
        <property type="entry name" value="BetaGal_jelly_roll_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008979">
        <property type="entry name" value="Galactose-bd-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031330">
        <property type="entry name" value="Gly_Hdrlase_35_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001944">
        <property type="entry name" value="Glycoside_Hdrlase_35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23421">
        <property type="entry name" value="BETA-GALACTOSIDASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13364">
        <property type="entry name" value="BetaGal_ABD2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10435">
        <property type="entry name" value="BetaGal_dom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13363">
        <property type="entry name" value="BetaGal_dom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01301">
        <property type="entry name" value="Glyco_hydro_35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00742">
        <property type="entry name" value="GLHYDRLASE35"/>
    </dbReference>
    <dbReference type="SMART" id="SM01029">
        <property type="entry name" value="BetaGal_dom2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117100">
        <property type="entry name" value="Beta-galactosidase LacA, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49785">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51011">
        <property type="entry name" value="Glycosyl hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="7" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="9">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172355" description="beta-galactosidase" evidence="9">
        <location>
            <begin position="19"/>
            <end position="1074"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-galactosidase" evidence="10">
        <location>
            <begin position="386"/>
            <end position="552"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009809"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP89968.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1074" mass="120810" checksum="2D4AB60413EC2F13" modified="2012-04-18" version="2">MNLRFALLALGLFRCGAALSERRDAQSQSVVSWDRHSILLRGERIFIQSGEFHTWRLPVVSQWTDILQKFTAAGLNTVSIYGKSIFNTLESIRGLVNPINGTTDWSGFKSLQPLFDAARSAGLFVIVRPGPYINAGGIPGWVTTLSCPLRTNATEFDRSWRQYWDEMIDHIVLNQIGLPNGTIIGVQVENEYLTDAHSSQGQPAGKDQYMQDLADALADKGIVVPLTVNDAGMDRNYATGRGSVDIYGFDSYPQSFDCSEPTRWKPVVEAYREYQDSLHLESPLFIPECAFSFWLIALTGPEFESVFYLNNLAANIKMINYYMLYGGTNWGQLAFPGTYTSYDYGSAISEDRTLNEKYTELKRQSLFLRSSKEFYETEVVGDSKRDPNYFDGESGNHAFVTELRNPTSGAGFYIVRALNSTSIKATKFRLNVLTSVGLKQLPEITLSPRQSRILVTDYQISSGSENLDGPRPIKLLYTTSSVLLSARIGGHDVLYLFSNGDEQASQDIEHAETSLLKIANLESGYWTRSFPEAGQASSFQISSQDNTDGYRKINWRIRDGDAIFALGAQDSLVLMTSKRMAGEVWNPILVQADSFSPPTSVLIWGPWLVRNASISMTENSKKSVLRIWGDLEEGLRKEVIIYIGGLAIGAVEWNGKKIHNISRAPQSDFLSFVYYPNSFKSSMDNKLNNQLTQVGSRIDLQTLHWSYRDSLPEIESTFSDEKWTEATIEDSNNHYKKLYGKYYLYACDYGFCNGATIWRGHFLHKCQSFFQDQHQVRLDVQKESPMLGINLTVAGGDFFAASVWLNDVFLGSLPNSTSKSDPKSYSKTLMNTFHFPPGSLKKCRANIITVVQDSMGMDQTEDGFSDTVKHPRGILGYKFEGRGVEDSLGEISWKVQGQLGGFYKLPDADRGIYNENGFYSIRSGWHLPGYDGQDSSKWRALSPISHGLKTAGIGFYRTIVKLDLEPGYDVILSFHFRQPQANQGRYRVEFWVNGWHMGKFVSHLGPQTRFPVHQGILNYQADNYFTLTLWALDEDGAKISGLELVVDKILEGGIGPIENHHPPYNHSMRNPLDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3L0B7</accession>
    <name>E3L0B7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_16184</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SLC35F solute transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178327">
        <property type="protein sequence ID" value="EFP89896.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334315.2">
        <property type="nucleotide sequence ID" value="XM_003334267.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L0B7"/>
    <dbReference type="FunCoup" id="E3L0B7">
        <property type="interactions" value="123"/>
    </dbReference>
    <dbReference type="GeneID" id="10545710"/>
    <dbReference type="KEGG" id="pgr:PGTG_16184"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16184"/>
    <dbReference type="HOGENOM" id="CLU_026578_1_0_1"/>
    <dbReference type="InParanoid" id="E3L0B7"/>
    <dbReference type="OrthoDB" id="1436450at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009262">
        <property type="entry name" value="SLC35_F1/F2/F6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23051:SF0">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23051">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35, MEMBER F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06027">
        <property type="entry name" value="SLC35F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="99"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="214"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="244"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="314"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="352"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="380"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="409"/>
            <end position="425"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP89896.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="429" mass="47131" checksum="A60C6B352605EAA8" modified="2012-04-18" version="2">MSSCLPRENSQHSQPHFLSPSSTYTYRLSLWIGYIHIRGIMIMNEPEPPSPPPVRGPSPRPAQDHYLVGIGLLLVVVALWVGSSFLMSSMFRDEEWNRPWLVTYICTSSFTLYLIRPGLSYLHTHGLSIKPLAPDPKLNARKNSHPASPVQAAYTLVDDQDRELSRSENRPTCVTRAFSVPESPLTTKEIAHLAATFVLLWFAANWSVNAALGYTSVSSTTILSSMSGFFTLAIGVATGAERFSLGRLLAVAMSVTGVVLVSKSDHSAYDPDPSDKTSSHWILGDVLALSSAALYALYVILMKVKVKEESRVDMQLFFGFVGAINMLCFWPMGVALHYTGIEPFSFPHTRKLWLSVILNAMCTFISDYIYMLAMLKTSPLVVTLGISLTLPVAVIGDIFKGIILPPTSLIGAGLVLSSFVILSLVEQQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>E3L2R4</accession>
    <name>E3L2R4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA helicase</fullName>
            <ecNumber evidence="2">3.6.4.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_17061</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.4.13"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="3.6.4.13" evidence="2"/>
    <dbReference type="EMBL" id="DS178338">
        <property type="protein sequence ID" value="EFP90862.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335281.2">
        <property type="nucleotide sequence ID" value="XM_003335233.2"/>
    </dbReference>
    <dbReference type="FunCoup" id="E3L2R4">
        <property type="interactions" value="949"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L2R4"/>
    <dbReference type="GeneID" id="10530768"/>
    <dbReference type="KEGG" id="pgr:PGTG_17061"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17061"/>
    <dbReference type="eggNOG" id="KOG0951">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000335_1_0_1"/>
    <dbReference type="InParanoid" id="E3L2R4"/>
    <dbReference type="OrthoDB" id="5575at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005681">
        <property type="term" value="C:spliceosomal complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003724">
        <property type="term" value="F:RNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000388">
        <property type="term" value="P:spliceosome conformational change to release U4 (or U4atac) and U1 (or U11)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18019">
        <property type="entry name" value="DEXHc_Brr2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18021">
        <property type="entry name" value="DEXHc_Brr2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000002">
        <property type="entry name" value="Activating signal cointegrator 1 complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000133">
        <property type="entry name" value="Pre-mRNA splicing helicase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.150:FF:000004">
        <property type="entry name" value="RNA helicase, activating signal cointegrator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000102">
        <property type="entry name" value="RNA helicase, activating signal cointegrator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000368">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein 200 kDa helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000012">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000024">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000004">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3380.10:FF:000001">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000062">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000254">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.20:FF:000013">
        <property type="entry name" value="U5 small nuclear ribonucleoprotein kDa helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.150">
        <property type="entry name" value="C2 domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.10">
        <property type="entry name" value="Sec63 N-terminal domain-like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041094">
        <property type="entry name" value="Brr2_helicase_PWI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048863">
        <property type="entry name" value="BRR2_plug"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035892">
        <property type="entry name" value="C2_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014756">
        <property type="entry name" value="Ig_E-set"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004179">
        <property type="entry name" value="Sec63-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057842">
        <property type="entry name" value="WH_MER3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF4">
        <property type="entry name" value="ACTIVATING SIGNAL COINTEGRATOR 1 COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21188">
        <property type="entry name" value="BRR2_plug"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18149">
        <property type="entry name" value="Helicase_PWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02889">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23445">
        <property type="entry name" value="WHD_SNRNP200"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039073">
        <property type="entry name" value="BRR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00973">
        <property type="entry name" value="Sec63"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81296">
        <property type="entry name" value="E set domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158702">
        <property type="entry name" value="Sec63 N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0347">Helicase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="534"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="12">
        <location>
            <begin position="757"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="11">
        <location>
            <begin position="1376"/>
            <end position="1560"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="22"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="191"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="410"/>
            <end position="431"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="2183"/>
            <end position="2211"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="24"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="55"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="75"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="225"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="410"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="10">
        <location>
            <begin position="2185"/>
            <end position="2202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP90862.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="2211" mass="250361" checksum="2ECF970DF5736423" modified="2012-04-18" version="2">MAPKNRDFSGYQYAAMSSLVLTADRSKIPRRDNEPTGEPETLVGRIDPKSMGSRAFKESVHIKKDISKKKSKHITSNEDRPKKFTETNSRRYADVIEAIQEVEGLNYKPRTNETRSIYELLLSSVQLMLGDQPNEIVRSATDMTIEAMKNDNDYPKDLDKKRTIEEFLGTISSEKFNELSNLAKKLTDYGDDDQAQQAGGEGEDGENQKRANELDDDNNGVAVVFEDDENGEDSDEDEFEIRDNSDSDEDSEADENEDQDDDDLNAQQTDPTDQIIIGPDSNNASDSKNKKGKQDLTIYDIDGFWLQRLIGNHFPDPIEAESKTKEAINLLSADNSSLRDLENSLVDLFDYDKFELVSVLTKHRDIIVWGTKWSRSDEDEKVNLAVVMREKGVGWIVKALTTGRGISNPQLQATANSNNKMDIDSESSKPTQFPTKANLEANSFLPNPKKVLDLSSMVFNQGSRTMTNKKCKLPEGSHKVPPTGQGYEEIHVPPPEKAPVKAEDLVKIVDLPHWSREAFKGATTLNRVQSKVFPVAFGQDDPILLCAPTGAGKTNVAMLTVLNEIAKHRNETTGEIDLAAFKIVYVAPMKALVQEMVGNFSSRLKYLGIQVGELTGDRQMTKDQITMTQIIVTTPEKWDVITRKSTDTSYTNLVGLIIIDEIHLLHDERGPVLEALVSRTIRRMEQNHEYVRLVGLSATLPNYADVARFLRVNPKKGLFFFDSSARPCPLKLEFIGITEKKAIKRLQLTNEICYEKVMKQLNDKQQIIIFVHSRSETTRTAKNLKETSIERDEVGLANREILMETAENVKDPGLKDILQFGIGIHHAGLERVDRRLVEELFADGHLQVLVSTATLAWGVNLPAHAVIIKGTQIYNPEKGRWVELSPQDILQMLGRAGRPQYDTFGEGIIITNHSELQFHLSITTSQLPIESQLVSKLADILNAEIVLGTIRNREEAAQWLGYTYWYQRALENPSLYGFQHDPEDPLLLQKRSDIVHTAFCILEKSGLAKYDRKTGLITTLELGKIASHYYVTNTSMSTYNQHLRPTMTLIELFRVFAASDEFKYIPTRPEEKQELAKLLEKVPIPVKESVGDPSAKINVLLQAYISRLPLEGFALMADMVYVTQSAGRILRALFEICLKRGWARLTHQALDLCKMVEKKMWVSMTPLRQFPSCSADIIRRAERKDFPWYRFFDLEPPELGELMGNPKLGKTIHRFVHQFPKLELQALVQPITRTMLRVELTITPDFMWEESVHGTAQMFWIMVEDVDGELILFSDQFLLRQRYANEEHFVTFYVPMIDPLPPNYFISVVADRWLHASTRLPLSFKHLILPEKFSQPTPLLDLQPLPVAALHNKAYESIYLKQGLKNFNKIQTQVFQALYTSNDNVLICSPTGSGKTICAEFALLRLWSQPEWQRCVCIEPYQEVVDLRVKEWRQKFGPLGKVIEPLTGELTRDVELTASDGSKPGQARIDIIICTPTQWDLVSRRWKQRKMVERTGLLIADEIHLIGSEIGPAYEVIVSRTRYVTAQSEISKTRIVALGCPLANARDLGDWMGANSQAIFNFAPGSRPLPLEVHIQSFNVPHFPSLMIQMAKPAYLSILEYAHEKPVIAFVPSRKQCRLTASDLSIYALSDEDPQRFLNIEQEDLAPYLAKVSDENLRETLASGIGYYHEAMSNTDKVIVQKLFEVGAIQVVIASKDTAWSIPMTAFMVIIMGVQNYEGKEHRYVDYSFPDILQMMGRACRPSEDSSSRCVLMCQQVRKEFLKKFLNEGLPIESHLHLSLHDHFNAEIVAKTIENKQDAVDWCTWQWFYRRLVANPNYYNMQATDHRHLSDHLSELVESTLSDLQNSNCIAIEDEMDTTPLPLGIVAAYYNINYITADVFSMSLTEKTKLKGILEIISAAQEFESIPLRHGEEGLLKKVHDRVPVKVGKVEYLSPHFKTNILLQAHFSRLTLPSDLMLDQVEILRKVPNLISAAVDVLSSQECLNTTVAMEFFQMVVQAVWNHDSPLKQIPGFSSEIIQRCTAANVNQVTDIMELEDEERNRLLQMDTKHLAKVAQFVNSYPAIEIKHEIEDEDSLVTNTPITLKVSLVAEDEEEEEGSKTAVAGNVVLAPFYPTVKQDCWWLIVEDPKQKKLLGLKKVTGATPLPTKIEFSVPTAGKHELKLDLISDSYIGVDQELRLEINIAEGEDSDSEESDDSDEEMQDQQASSEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3L2X6</accession>
    <name>E3L2X6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178338">
        <property type="protein sequence ID" value="EFP90901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335320.1">
        <property type="nucleotide sequence ID" value="XM_003335272.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L2X6"/>
    <dbReference type="GeneID" id="10530809"/>
    <dbReference type="KEGG" id="pgr:PGTG_17100"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17100"/>
    <dbReference type="HOGENOM" id="CLU_071663_0_0_1"/>
    <dbReference type="InParanoid" id="E3L2X6"/>
    <dbReference type="OMA" id="DFLMTIT"/>
    <dbReference type="OrthoDB" id="2147986at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.340">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="56"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="233" mass="26617" checksum="423536721F18747B" modified="2011-01-11" version="1">MSGQLFIGPDHNGKTPYLEARYKPLNLLTTLKEIRPFLLGTLEYAKQPQTKLKKLMKDKNSKIDSVTQEMQNMKTNVAKMEEKLAKTEQELKITKEKVENTEMMNAVYDFVHSIERIVCRLLNFDEHRSMTINQALKSGATDWSKLQNLLKLEDQSPECLLTTINKIKDQRLGCDHASKSTAKDLVLSTSLITISQDHFSLRHPELDMLQNLLKWSSSLLPASATLDNLKKEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3L2Y1</accession>
    <name>E3L2Y1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ysc84 actin-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_17178</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178339">
        <property type="protein sequence ID" value="EFP90906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335325.1">
        <property type="nucleotide sequence ID" value="XM_003335277.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L2Y1"/>
    <dbReference type="STRING" id="418459.E3L2Y1"/>
    <dbReference type="GeneID" id="10528321"/>
    <dbReference type="KEGG" id="pgr:PGTG_17178"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17178"/>
    <dbReference type="HOGENOM" id="CLU_015320_1_1_1"/>
    <dbReference type="InParanoid" id="E3L2Y1"/>
    <dbReference type="OMA" id="PTATGDH"/>
    <dbReference type="OrthoDB" id="443981at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030479">
        <property type="term" value="C:actin cortical patch"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051015">
        <property type="term" value="F:actin filament binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051666">
        <property type="term" value="P:actin cortical patch localization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051017">
        <property type="term" value="P:actin filament bundle assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11525">
        <property type="entry name" value="SYLF_SH3YL1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051702">
        <property type="entry name" value="SH3_domain_YSC84-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033643">
        <property type="entry name" value="SYLF_SH3YL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007461">
        <property type="entry name" value="Ysc84_actin-binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15629">
        <property type="entry name" value="SH3YL1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15629:SF7">
        <property type="entry name" value="YSC84 ACTIN-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04366">
        <property type="entry name" value="Ysc84"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ysc84 actin-binding" evidence="2">
        <location>
            <begin position="121"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP90906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="293" mass="30418" checksum="C81A22558BE667A2" modified="2011-01-11" version="1">MSLMERFQSAAKKVGVQATAFSRDVATMASDGGRQLVTEFKLESECERSAKILSSFLADPLHPESALNAIPKAVLQNAHGLAIFTILKVGFVWSGKAGSGIVIARLEDGSWSAPSCIATGGVGFGLQVGADFSEFVIVLNSEEAVRAFATTGNMTIGGNLSAAVGPIGTGGAVNASLLHPAPLFTYSKNKGLFAGLSLEGTALIERKDTNEAFYGQRIPSLDILRGRVPPPEAASALYEVIESAEQLDESNLPQESYVPNGGVNDNTTPGSASKEAGSTPTGNTNLFDAGDAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3L446</accession>
    <name>E3L446_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_16975</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178345">
        <property type="protein sequence ID" value="EFP91321.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335740.2">
        <property type="nucleotide sequence ID" value="XM_003335692.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L446"/>
    <dbReference type="GeneID" id="10529153"/>
    <dbReference type="KEGG" id="pgr:PGTG_16975"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16975"/>
    <dbReference type="HOGENOM" id="CLU_1090455_0_0_1"/>
    <dbReference type="InParanoid" id="E3L446"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP91321.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="255" mass="28653" checksum="40D18AB8E18F3304" modified="2012-04-18" version="2">MSAIIQRQISDQTALANNISFCLFFVLVRPKPLFVNPHKPCPNSPGLFRFHFQARLNLFKPLLNWETEPIIAGLGALLTTYLFLHTHSTSGLNDLVLVRPLIYQTLNFISAKGHISPFQGTFFIFSSSYSEVPAMVRKKQKTAHGPAKPPEGGARGSSYNDTNALLEMMCKRLMKQRAVAQVVSVDFDLLAVFLMQEFQEMDIQVIVEEVLRVFSQHAPQTMLQNQVFDAAKEVDQTIRCGTAQLMSEELGGDKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>E3L494</accession>
    <name>E3L494_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">E3 ubiquitin ligase complex SCF subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_17023</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <text evidence="3">Essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the SKP1 family.</text>
    </comment>
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        <property type="protein sequence ID" value="EFP91369.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335788.2">
        <property type="nucleotide sequence ID" value="XM_003335740.2"/>
    </dbReference>
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    <dbReference type="FunCoup" id="E3L494">
        <property type="interactions" value="479"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L494"/>
    <dbReference type="GeneID" id="10529282"/>
    <dbReference type="KEGG" id="pgr:PGTG_17023"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17023"/>
    <dbReference type="HOGENOM" id="CLU_059252_4_0_1"/>
    <dbReference type="InParanoid" id="E3L494"/>
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    <dbReference type="UniPathway" id="UPA00143"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
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    </dbReference>
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        <property type="term" value="C:nucleus"/>
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    </dbReference>
    <dbReference type="GO" id="GO:0097602">
        <property type="term" value="F:cullin family protein binding"/>
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    </dbReference>
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        <property type="term" value="P:mitotic cell cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031146">
        <property type="term" value="P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd18322">
        <property type="entry name" value="BTB_POZ_SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.710.10:FF:000026">
        <property type="entry name" value="E3 ubiquitin ligase complex SCF subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016897">
        <property type="entry name" value="SKP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001232">
        <property type="entry name" value="SKP1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036296">
        <property type="entry name" value="SKP1-like_dim_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016072">
        <property type="entry name" value="Skp1_comp_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016073">
        <property type="entry name" value="Skp1_comp_POZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11165">
        <property type="entry name" value="SKP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01466">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03931">
        <property type="entry name" value="Skp1_POZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF028729">
        <property type="entry name" value="E3_ubiquit_lig_SCF_Skp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00512">
        <property type="entry name" value="Skp1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54695">
        <property type="entry name" value="POZ domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81382">
        <property type="entry name" value="Skp1 dimerisation domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0833">Ubl conjugation pathway</keyword>
    <feature type="domain" description="SKP1 component POZ" evidence="5">
        <location>
            <begin position="1"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="domain" description="SKP1 component dimerisation" evidence="4">
        <location>
            <begin position="108"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR028729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP91369.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="158" mass="18027" checksum="344721669D9510C1" modified="2012-04-18" version="2">MVLMVTSDGEEFIVEKEVATRSALIKNMIEDLGESDNPIPLPNVSASVLKKVLEWCEHHKKDPEPSAEDPDDARKRATEISDWDTKFINVDQEMLFEIILAANYLDIKPLLDVGCKSVANMIKGKQPEEIRKLFNIANDFTPEEEAQIKKENEWAEDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3L6B2</accession>
    <name>E3L6B2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18177</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178357">
        <property type="protein sequence ID" value="EFP92087.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336506.2">
        <property type="nucleotide sequence ID" value="XM_003336458.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L6B2"/>
    <dbReference type="GeneID" id="10532045"/>
    <dbReference type="KEGG" id="pgr:PGTG_18177"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18177"/>
    <dbReference type="HOGENOM" id="CLU_068104_0_0_1"/>
    <dbReference type="InParanoid" id="E3L6B2"/>
    <dbReference type="OrthoDB" id="2507547at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="260"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="333"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="360"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="370"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92087.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="379" mass="42419" checksum="B0C2D77B1A1D0C99" modified="2012-04-18" version="2">MPATHSSTQRRSSLKCPALSHNPIRSLSLPTHHEKPQKSNRVRFAICTRAPQAETPRRPLNAPGSKQQTRVKVGQLAPTRIPCRIQSGRPNGISPRARKERSQAERNQRSKTLQATQRAHQRPRRVSSSHLERTRASSDGQYPNATLPLYIPSHSRAALSPCLSSPSKINCSISSSNPGGCEKLLQKIHRPVPASIDHNQKPACQDSVMDLGLKAQSKAYTLSPSWCSPNIHSAVHKYNVSRSKTQTTDNEILSIFLKNNGGYLDDSDDEDDEEDEEDEEEDSEEAEESFTSVRATVAFLKRIPLYPFEFRKPTSLSYSEQFNDLSLLDISTSRSASDSSTSPSQAHSSPPRYYWNRVLPTTPVTPTPKTRSRFTFYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>E3L7X3</accession>
    <name>E3L7X3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_18826</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP92648.1"/>
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    </dbReference>
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    <dbReference type="KEGG" id="pgr:PGTG_18826"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18826"/>
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    <dbReference type="InParanoid" id="E3L7X3"/>
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP92648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="32" mass="3733" checksum="EBF91138746CD49B" modified="2011-01-11" version="1">MPKMLKDSEKRLPFFRQRLPTTVSGRPLGVVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3L7Y0</accession>
    <name>E3L7Y0_PUCGT</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_18833</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178369">
        <property type="protein sequence ID" value="EFP92655.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337074.2">
        <property type="nucleotide sequence ID" value="XM_003337026.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L7Y0"/>
    <dbReference type="GeneID" id="10538983"/>
    <dbReference type="KEGG" id="pgr:PGTG_18833"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18833"/>
    <dbReference type="HOGENOM" id="CLU_484086_0_0_1"/>
    <dbReference type="InParanoid" id="E3L7Y0"/>
    <dbReference type="OrthoDB" id="2495627at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052980">
        <property type="entry name" value="Crinkler_effector"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33129:SF1">
        <property type="entry name" value="ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33129">
        <property type="entry name" value="PROTEIN KINASE DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP92655.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="503" mass="56977" checksum="C09FC73401EF2FC7" modified="2012-04-18" version="2">MVPPKIYLSFKNQVFAVETKGLLIVRELAQAAGKEFPDELRGSISQITLHTDEDADALPPHACLAEHAEQLFRCSVPETPLVVKVKSGVAPKTPLTAKGIPAIIWLSYNNELTSVRTQELEFVDHLAKAARNKFSPYLRDFATPDITLHVDERAEALATDSVLAEIAAQLSRCSIPETPLIVKVPEFKAGSKVEEGLIRFWKALPEASLVVNGEVEYLELKEAYILGDKKLGHRLLVRPIYKELCELFEVEGYDQWVVTGTPGIGKTFFSVYYMWIAARKKKAVLWQNPFFPDDVCYLMASSGIVPVHTWGSEIDGVLAKFDPVHIVDGRAPKLRNVWTLLVTSPQHNHYHQFLKDDNSKLLYMPPWSYEELQTCKAIFYPDERILPTTLMDRVFEWYGGVPRYVLARASTEFKRKGGDEDAAFRAVNQRLTEAIYRGGIMDVMKAYQAKTPDGQFSHRVVHIYSHPSGELTRFHLSWASHQVESAMTKRYEQELCIDLPNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3L985</accession>
    <name>E3L985_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Tyr recombinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_19215</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178382">
        <property type="protein sequence ID" value="EFP93110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337529.1">
        <property type="nucleotide sequence ID" value="XM_003337481.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L985"/>
    <dbReference type="STRING" id="418459.E3L985"/>
    <dbReference type="GeneID" id="10543175"/>
    <dbReference type="KEGG" id="pgr:PGTG_19215"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19215"/>
    <dbReference type="HOGENOM" id="CLU_003292_5_0_1"/>
    <dbReference type="InParanoid" id="E3L985"/>
    <dbReference type="OMA" id="NDPKWEN"/>
    <dbReference type="OrthoDB" id="5149081at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.443.10">
        <property type="entry name" value="Intergrase catalytic core"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Integrase-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052925">
        <property type="entry name" value="Phage_Integrase-like_Recomb"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34605:SF3">
        <property type="entry name" value="P CELL-TYPE AGGLUTINATION PROTEIN MAP4-LIKE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34605">
        <property type="entry name" value="PHAGE_INTEGRASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP93110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="350" mass="39626" checksum="6368D081B82C4454" modified="2011-01-11" version="1">MEMVDSSEDIVIVDNQPLTVGHLNKIKAFAANGTTHYPISTLNAHLLSGWKWNTILGYNAAVKKFIFFKNLSGNHNFKLPASKKDIEDFCFWAGRNLYLNNSYKISAPSIKKYLCGLKAWHTFHNEEYPEIADKRIELLLKASFRIDATLPKKPKKPPILLKHLIWLSNALFPGGDIDKTIMDLAIVSFWGMARLGEFTYDNPFGDLNSENSVLTSDVRLVRSPIGEKAIITVRNAKTAQPGKPQEIVVHSLKNMLCPVLALKRRLEEVNGTDTSLFGFYRDGVRRHLTRPISVNRIQLVLRVGGFEGLLGHSFRVGGASLRLYIREYTAEDKEDSKKIIAELNELWEED</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3L9L7</accession>
    <name>E3L9L7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19188</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178386">
        <property type="protein sequence ID" value="EFP93242.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337661.2">
        <property type="nucleotide sequence ID" value="XM_003337613.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L9L7"/>
    <dbReference type="GeneID" id="10543474"/>
    <dbReference type="KEGG" id="pgr:PGTG_19188"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19188"/>
    <dbReference type="HOGENOM" id="CLU_2016356_0_0_1"/>
    <dbReference type="InParanoid" id="E3L9L7"/>
    <dbReference type="OrthoDB" id="10529866at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="44"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93242.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="123" mass="13666" checksum="9E0C39F653A60D32" modified="2012-04-18" version="2">MALLFGAQGRRRSRQFDGEGCHALCGASKEPVDKRSYLLGSDLSPGAWHQGGQGKNHGPAWNIKPNPINRTDKSRADNALHCLDYKPAPVKSLDGLQKSLIAPSLDHFNDWGHQSRHRPRVRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3L9X2</accession>
    <name>E3L9X2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_19302</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178389">
        <property type="protein sequence ID" value="EFP93347.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337766.2">
        <property type="nucleotide sequence ID" value="XM_003337718.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L9X2"/>
    <dbReference type="STRING" id="418459.E3L9X2"/>
    <dbReference type="GeneID" id="10543693"/>
    <dbReference type="KEGG" id="pgr:PGTG_19302"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19302"/>
    <dbReference type="HOGENOM" id="CLU_897524_0_0_1"/>
    <dbReference type="InParanoid" id="E3L9X2"/>
    <dbReference type="OrthoDB" id="10269915at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP93347.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="324" mass="36810" checksum="C438A13FF3779C99" modified="2012-04-18" version="2">MTLQLDSTGIRTLTMHEEVDTHGRTRPEEVDDIEIPPLMGNTLTEAELQEFYPELAEWPCTTTASEDEGPGASVPPEVPAPLSVAIILEYHCWLPSPQADQDGCRQYRAAHGVQTWTETIGPSRLVTWRGDPRASTWDQIRCDVLEAFITYDTEVADELRSNDRYRDLLWEGTIPDLPEFGRGVPFRICTDVCWGIFTDYIARTTATSKIYLRLFTNRPYGRTAVSDAFRRAMHIRHCPHRNNLIAELERRLHTEATHLDGNSGHIKLKVKWATGGQKFIRMSAAVIHGWARAIVSNPSLVTLDTPPVGREYLWEWVHAHAQLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3LB17</accession>
    <name>E3LB17_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19726</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178406">
        <property type="protein sequence ID" value="EFP93742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338161.1">
        <property type="nucleotide sequence ID" value="XM_003338113.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LB17"/>
    <dbReference type="GeneID" id="10535112"/>
    <dbReference type="KEGG" id="pgr:PGTG_19726"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19726"/>
    <dbReference type="HOGENOM" id="CLU_831913_0_0_1"/>
    <dbReference type="InParanoid" id="E3LB17"/>
    <dbReference type="OrthoDB" id="2506868at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="283" mass="32090" checksum="2DED7ADD5A281BA8" modified="2011-01-11" version="1">MFVSRAALLTIVFGSPLQHGFVVGMLLGLLLIALLYISRYVLSPLQKKALAAKSKIIPNFIPPDYFPPIQFRNGSKNLGIFDSIITFSQYGPNDLEFTDYDRLAHTYSSPKFSSIIDIELISSLKRTGRVIRSLNDHLHTLGIDGFNAMLVFVFQMIQVTRLLTNSALSHHDELINGFNQSIQKIEQARTETSQQQRRLLAQLQRSVDLAFAVRSGLERWRRQLKLYGINVLSTQAFDQSGGMARSYLQDMVAQALDSLLVSKEHKEGLLVAMREDQKRRFND</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3LBV0</accession>
    <name>E3LBV0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19772</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178428">
        <property type="protein sequence ID" value="EFP94025.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338444.2">
        <property type="nucleotide sequence ID" value="XM_003338396.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LBV0"/>
    <dbReference type="GeneID" id="10535638"/>
    <dbReference type="KEGG" id="pgr:PGTG_19772"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19772"/>
    <dbReference type="HOGENOM" id="CLU_104304_0_0_1"/>
    <dbReference type="InParanoid" id="E3LBV0"/>
    <dbReference type="OrthoDB" id="10386439at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP94025.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="182" mass="19524" checksum="02B97209A5285F89" modified="2012-04-18" version="2">MLCEQIKVENATTLAPSQRQQALDDHRQDGRSAVHSPSTATSSTLGNSSPIRPSRSSELASSLARASFKLNATQFHALSGDFNKLSSFITQNHLNQHHPTTTGDEIVQVEDGMSCGMAEISELHDWVDGLLTPALAEVKFSYRTTNELTAAWAAAASLSLASFARGIAIFCSISGCIQRSCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3LBX0</accession>
    <name>E3LBX0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20006</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178431">
        <property type="protein sequence ID" value="EFP94045.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338464.2">
        <property type="nucleotide sequence ID" value="XM_003338416.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LBX0"/>
    <dbReference type="GeneID" id="10535738"/>
    <dbReference type="KEGG" id="pgr:PGTG_20006"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20006"/>
    <dbReference type="HOGENOM" id="CLU_894693_0_0_1"/>
    <dbReference type="InParanoid" id="E3LBX0"/>
    <dbReference type="OrthoDB" id="10513609at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="229"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP94045.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="311" mass="33769" checksum="879517587C9F0148" modified="2012-04-18" version="2">MKLLEYSHNESIKQTAEVKRGNDIQAELVAINQAKKNLNCMLQNLDDCPDDLAREYLASEKQRIMEERRLAMKKSSSSRPQPSVEEDGESDEDKDGDMTQTQNITSQTGNQELPIRDGRLASGYPRGCGYPAAFSGHLSIQIPHPQPAGGYPSATAGILGYPHSKGPAGRASFSQRGTCTHRLEGCPPIDEVLVPRRPEGLPSAGEVLVLVDWKGCPSSRRAGEVLVPRQPEGCPSSRQGTCTSPAGRRPFQPAIYPSGRGYPLGYPDTRQIFEEKGPSAPVGGYPLALAGIRQRITGIRDRYPSTISTSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>E3NY12</accession>
    <name>E3NY12_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20417</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS990008">
        <property type="protein sequence ID" value="EFP94461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338880.1">
        <property type="nucleotide sequence ID" value="XM_003338832.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NY12"/>
    <dbReference type="GeneID" id="10527754"/>
    <dbReference type="KEGG" id="pgr:PGTG_20417"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20417"/>
    <dbReference type="HOGENOM" id="CLU_1807130_0_0_1"/>
    <dbReference type="InParanoid" id="E3NY12"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP94461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="152" mass="16822" checksum="33B84F4656C1BCC7" modified="2011-01-11" version="1">MNGSKINNSRPGRTLFGCEHEQHKRRTLGAVVIIRHVQGVLQFTTNLCSIAGHPKSLASDDSVPLELYKRGIAGVTPAQGLLLRQPTSTPDVRRAEASGTTDSMKESRSSFCYTPRMQYIGRQTTVQGHCHATALPPQRHLQSSDYVRVPYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>H6QNS8</accession>
    <name>H6QNS8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20636</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EHS62515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890832.1">
        <property type="nucleotide sequence ID" value="XM_003890783.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QNS8"/>
    <dbReference type="GeneID" id="13542513"/>
    <dbReference type="KEGG" id="pgr:PGTG_20636"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20636"/>
    <dbReference type="HOGENOM" id="CLU_2484419_0_0_1"/>
    <dbReference type="InParanoid" id="H6QNS8"/>
    <dbReference type="OrthoDB" id="2508042at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="87" mass="9564" checksum="972E761445CC5889" modified="2012-04-18" version="1">MKLTIAVHSIFYTLVFMIMTSTMTVEGLRCRNPALSQPVCTEGSNYPKAIIDKENKTFTCPGSSSPRCCNYDPAEDPPTISCTPKVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>H6QNX5</accession>
    <name>H6QNX5_PUCGT</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20571</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EHS62445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890762.1">
        <property type="nucleotide sequence ID" value="XM_003890713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QNX5"/>
    <dbReference type="STRING" id="418459.H6QNX5"/>
    <dbReference type="GeneID" id="13542572"/>
    <dbReference type="KEGG" id="pgr:PGTG_20571"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20571"/>
    <dbReference type="HOGENOM" id="CLU_023058_0_0_1"/>
    <dbReference type="InParanoid" id="H6QNX5"/>
    <dbReference type="OrthoDB" id="2510773at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="407"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="453"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="648"/>
            <end position="705"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="412"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="453"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="476"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="537"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="568"/>
            <end position="604"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="612"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="663"/>
            <end position="697"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="732" mass="79747" checksum="3C65D5775B87127B" modified="2012-04-18" version="1">MGGITLRGAMDNLYTPDEPPPEHTSHYHDPTGFQPLYSRLNHQNTGVWSETAGTQQSQHPVPGELNHTRYGHTPVNQQGGNFSVAPVNLGTRTAASVPRGPALGQGRLTHTASETYSHHANTGIQSGHYEARTQAPQSNTGIQSGNYEASTQAPHLQQQSVQFTQGTPATTFRPDLQINDPHTAPLPPVVDPALPPVHPPAATIRPATGVGRTRKPKRSPEEMRIAQVVAAEKRARRFQMQAEKAADARQKQATNAANKLIKAQKAASATPRPTWNEEQTLELLNYVRMVKEDHSQTRVTGGFIPFGKYFAAYTGREDAFPLLESISTATRLAKYRAVMDKWKRVKDVVDRSGAGGLSSALEAGGVSLEVWDLILDMHGDNPAATGDGLTSSYADYESLLQEEVISETSEVDGSSFSDDAGADSPPATPAASKGRQTKYQRLCAGLTPAELALDADSDSGSDLPEELVRRPEATVPSASTPVGPTTVHTVQAAVGTPASTPGGARKGSKGKAKSSTSKALISWPPAEGGSVPRRRGRTEEKAKEEDHAGTGMLVMMHKAQEASAAWAAEDRQQRMEERTRQDTLRSEERQERQAELRSLERLRGQELQAAAKRAEEKEAERAEAVRQADEDRHLLRQQARLDRELADHQMQMDRQAAQLRATEAAEDRKREREEAVARKAEERKREDEREERAERQQQSRQLFEMAMLKAMGLSIALPDGSPRAGGSGGQGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQ11</accession>
    <name>H6QQ11_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20957</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EHS64494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890408.1">
        <property type="nucleotide sequence ID" value="XM_003890359.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQ11"/>
    <dbReference type="GeneID" id="13542723"/>
    <dbReference type="KEGG" id="pgr:PGTG_20957"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20957"/>
    <dbReference type="eggNOG" id="ENOG502S1WT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018307_2_0_1"/>
    <dbReference type="InParanoid" id="H6QQ11"/>
    <dbReference type="OrthoDB" id="2497597at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="282"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="377" mass="41729" checksum="75D0622930BD74CF" modified="2012-04-18" version="1">MSAHQANTARISRLEELILAMNIKNKADAQPVQTSPGRVDLQKFCTSDGPIYRGLFQVVEPFLRWIHRVQIFFDTKDVNNSADRIKILGNLIAETNLQSFYANEASGFLTKSWNDFKTRMFNFALPTNWRSGLQRQIRKLKMSQAETFLDFDANETSKLGNLQLAQFLVYGLPDALQDRINKRQLLEVVPFEKQANSSFLALHCTTETPAPVRTTPSAPSTLARDEFVWRVHSYLNSQGLCHFCKKHCGSANGSCPGPINRSRIDIPSSFITPTKPPDYVAPRAWGKPAAAPGKSTQAPAGRPSTRAASVAGITDTTPLEAQVSALTLDAAMREDGRWDTYFDDEGCFPSLEWEGTGPTGDVANVQARECLEAARTG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQA3</accession>
    <name>H6QQA3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_21067</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EHS64809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890330.1">
        <property type="nucleotide sequence ID" value="XM_003890281.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQA3"/>
    <dbReference type="GeneID" id="13542142"/>
    <dbReference type="KEGG" id="pgr:PGTG_21067"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21067"/>
    <dbReference type="HOGENOM" id="CLU_278007_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQA3"/>
    <dbReference type="OrthoDB" id="2506207at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="24"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="199"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="333"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="501"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="539"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="607"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="698"/>
            <end position="718"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="875"/>
            <end position="979"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="995"/>
            <end position="1077"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="199"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="227"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="241"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="276"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="336"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="354"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="511"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="539"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="553"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="578"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="607"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="622"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="917"/>
            <end position="928"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1009"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1060"/>
            <end position="1070"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS64809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1139" mass="122450" checksum="0F5733547927CF88" modified="2012-04-18" version="1">MLFRVASLVAVIHCEPHHISARRVSVIGSSILSLSLFSLLLLSALVQLTPRRPPMSEALPEDPSIEQIASNIHLAELLASGGGADVSSLSTTHQSQEEIRSLLENHLTSHNKSVQVGLLERYMRICRKSSTNYPPFPITGIKACLYILKVSTDKTDVSIKEKLAVVIETWRATLEQAIGPVFAGSPSIWTCKALTQLTGRSENSTNSPITNSGKRKRSQDSLSGVNSQLIPSSSPLPQRCRTASSRISESLLSQQSPSHLTPNNLSGPKDPKEVTICSNLLPSESSQPTSMKTITNHKQDPVNSSSNDPVKTVQLPLLQTSSSAAPVTSCPLLSPSVGSSSALQPSQVHMPLPNTLSSQVANPKTGPQPSLDSQSQISRVTQNINHRIEAPTSAANHLANERDKVSRILPVPNCLSANPPIPAQADKWQRAFRRLDLAVTELLESIPLTPVVSLSESSFYRPIEFSPAFQWTPPSVSSYVNPPGLSLTNIPPIVTFRPQLELHSPPAKPSNVPTPDSSRQTNLGMIRIASEKQMSNAFSFPPVTLNNTTHGKPPTPASPAITPLPPTRPSSTIQPAPSSSLVYSNQSSSIQPNLHQFSQLPGNVQVNNIQPSLQQPGQLPVNVQSNSIQPSPQQSSQSPGNVPVEPQPIFNVTVAQKMKFVDTYRRVDASGKAKLEATLRKNKLWDVLAPLLTPSAIGTPTATTTPQNASPAPQPQVNAVSTPLNQTTVQNSLSSTPMLHSTPKKSDSASTVVANAHVPAVATPKQFDSCTVNSESSNAISQSASPQVYQGSIHANYLNSAAPFNDGALDPVLTKMILAMLAAEPQATNVLMVAFESLGIRNRDAIMTHLKALAVPVNGVDDFWQLRTQAGLASPATVLPDSPSIASHPQLTPKPVNPITVPTMQSKHAPQPDRPLTNGTIDKSTYAASNPLLHPSAKQDSPSRLGQPKAHPQDQNAVGHGTKEHSRENPIQSSKAMNGARTEAMDEIEVLEVVDRQKTNKATSATDMVSEKKHSSDPAKKRMTPISSEWESKKLAFMGDGESSKRPSSIGRRGEETPVNDCQASSSSTKKPPHRFQVSKDMTGRHHFNILDDGLPGFPDVDPSFQIHGAPARVLARYPPDQQMPPVRTFVPLLKKSQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>H6QR37</accession>
    <name>H6QR37_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21304</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EHS63012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890016.1">
        <property type="nucleotide sequence ID" value="XM_003889967.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QR37"/>
    <dbReference type="GeneID" id="13540942"/>
    <dbReference type="KEGG" id="pgr:PGTG_21304"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21304"/>
    <dbReference type="HOGENOM" id="CLU_1759714_0_0_1"/>
    <dbReference type="InParanoid" id="H6QR37"/>
    <dbReference type="OrthoDB" id="10352979at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="148" mass="16682" checksum="EC212060D461F5B1" modified="2012-04-18" version="1">MYSRYSTPDYTLDYDYGSKRLTEPEPTQVAQPSPNDMVVDEPVIEEIKPNVKMASGLSGQVAVNPMQTDVVPQAVNIDNMSKIMISKGLEIVKTPSFYFPLKAKWHSIYDPQGRYDLGLGARCKKNEREEIIELTHQLSDYSGTFEAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRM8</accession>
    <name>H6QRM8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21626</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178284">
        <property type="protein sequence ID" value="EHS63322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889825.1">
        <property type="nucleotide sequence ID" value="XM_003889776.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRM8"/>
    <dbReference type="GeneID" id="13540760"/>
    <dbReference type="KEGG" id="pgr:PGTG_21626"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21626"/>
    <dbReference type="HOGENOM" id="CLU_2543689_0_0_1"/>
    <dbReference type="InParanoid" id="H6QRM8"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="83" mass="8785" checksum="E7F9B01935AE3776" modified="2012-04-18" version="1">MWAEGIGSSLDWSDHTTGQSKSACGPERQLLRGVRESACRATTFHGVGRDPTAPCLSELANLSCCVRVGVPMNVCGLADENYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRT4</accession>
    <name>H6QRT4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21481</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EHS63407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889776.1">
        <property type="nucleotide sequence ID" value="XM_003889727.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRT4"/>
    <dbReference type="GeneID" id="13543092"/>
    <dbReference type="KEGG" id="pgr:PGTG_21481"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21481"/>
    <dbReference type="HOGENOM" id="CLU_2655623_0_0_1"/>
    <dbReference type="InParanoid" id="H6QRT4"/>
    <dbReference type="OrthoDB" id="2495758at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="76" mass="8830" checksum="81B1D83749FE568D" modified="2012-04-18" version="1">MVRRRASAPSALMLQPATVPLIRGQPKFILPSPPSEYAIIHLADHRKLSDNYQKYRLEPLKELPRLKDFQVDYIKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>H6QS52</accession>
    <name>H6QS52_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrotransposon Copia-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21700</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="1" key="1">
        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="1">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EHS63526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889671.1">
        <property type="nucleotide sequence ID" value="XM_003889622.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QS52"/>
    <dbReference type="GeneID" id="13542882"/>
    <dbReference type="KEGG" id="pgr:PGTG_21700"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21700"/>
    <dbReference type="HOGENOM" id="CLU_199246_0_0_1"/>
    <dbReference type="InParanoid" id="H6QS52"/>
    <dbReference type="OrthoDB" id="2802833at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="59" mass="6625" checksum="DCF22D2A584C1577" modified="2012-04-18" version="1">MPYRTTQNPSPLIPSVPQLDGNSVTFTSWRSRLEDVLAIQGVLDIVQGKIPRPLWNFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSA6</accession>
    <name>H6QSA6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">High-affinity iron permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_21721</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the oxidase-dependent Fe transporter (OFeT) (TC 9.A.10.1) family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EHS63630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889615.1">
        <property type="nucleotide sequence ID" value="XM_003889566.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSA6"/>
    <dbReference type="FunCoup" id="H6QSA6">
        <property type="interactions" value="48"/>
    </dbReference>
    <dbReference type="STRING" id="418459.H6QSA6"/>
    <dbReference type="GeneID" id="13542632"/>
    <dbReference type="KEGG" id="pgr:PGTG_21721"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21721"/>
    <dbReference type="eggNOG" id="ENOG502QQWE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_046738_1_1_1"/>
    <dbReference type="InParanoid" id="H6QSA6"/>
    <dbReference type="OrthoDB" id="4364at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033573">
        <property type="term" value="C:high-affinity iron permease complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034755">
        <property type="term" value="P:iron ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004923">
        <property type="entry name" value="FTR1/Fip1/EfeU"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31632">
        <property type="entry name" value="IRON TRANSPORTER FTH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31632:SF2">
        <property type="entry name" value="PLASMA MEMBRANE IRON PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03239">
        <property type="entry name" value="FTR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0410">Iron transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="105"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="223"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="256"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="287"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="370"/>
            <end position="390"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS63630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="425" mass="47051" checksum="792AD73A3962B181" modified="2012-04-18" version="1">MPANLFSVPIFLICFREVLEAAIILSVLLALIEQIVRPQGHSPTPTKDQLSIETPRLPRLEENLNESHLELESIDSRQATHPIPGILKNHGDYRDRIIKRMRVQVFSGTFVGLFLSLCIGAGFLAVYYTKFTDIYGNAEELWEGIFSLIASGMIFVMGITMMRMERSKIKWRLKLSKAFEQENIIDVDGKAPKLHSKFRTLLSMLRVGGSRRTNSADSTSSRYILFFLPALTVLREGLEAVVFIGGVSLGQPATSIPIAAVTGILLGLGIGYAIYNSSARLNLSLFLMISTIALMYVGAGLITKAAWYFKMYTFIRSVGGDAAEAGDGPGSFPVDGYIWHLDYGNPENKLAAGGWMIFAGVFGWNNTGSILTVMIYISFWLISSATLIFMKWHEGRCTIFNISSSARKARLGRLDHGGKDNQLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTD2</accession>
    <name>H6QTD2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4219 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21901</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178311">
        <property type="protein sequence ID" value="EHS64151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889231.1">
        <property type="nucleotide sequence ID" value="XM_003889182.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTD2"/>
    <dbReference type="GeneID" id="13541703"/>
    <dbReference type="KEGG" id="pgr:PGTG_21901"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21901"/>
    <dbReference type="HOGENOM" id="CLU_103048_1_0_1"/>
    <dbReference type="InParanoid" id="H6QTD2"/>
    <dbReference type="OrthoDB" id="2518500at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="151" mass="16786" checksum="90AAF1E2E924837C" modified="2012-04-18" version="1">MDTINPTILKTPIEAIPVLTKENFSTWRARITALFKLGGLKDQILEGEPALKDNDNTILCAIILAKLSTTTHNNVVNSTNEADAIELLKNIQKRFVSTEPSNQARVYNLFANIAFDASNIKTFITKVRSALVKMKEVGVIIPKDILTYDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTG0</accession>
    <name>H6QTG0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22085</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178312">
        <property type="protein sequence ID" value="EHS64175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889220.1">
        <property type="nucleotide sequence ID" value="XM_003889171.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTG0"/>
    <dbReference type="GeneID" id="13541734"/>
    <dbReference type="KEGG" id="pgr:PGTG_22085"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22085"/>
    <dbReference type="HOGENOM" id="CLU_2868664_0_0_1"/>
    <dbReference type="InParanoid" id="H6QTG0"/>
    <dbReference type="OrthoDB" id="10379320at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="64" mass="7192" checksum="66B4C8349CF4BB99" modified="2012-04-18" version="1">MFRREVFMPGGCRRGTAVHTLFSEGNDGQSRESPPDKSRGFRRSDSPPEFLPPQGVGIIFRRAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUP2</accession>
    <name>H6QUP2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">EXS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_22473</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="8" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178348">
        <property type="protein sequence ID" value="EHS64754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888762.1">
        <property type="nucleotide sequence ID" value="XM_003888713.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUP2"/>
    <dbReference type="STRING" id="418459.H6QUP2"/>
    <dbReference type="GeneID" id="13541630"/>
    <dbReference type="KEGG" id="pgr:PGTG_22473"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22473"/>
    <dbReference type="HOGENOM" id="CLU_1396977_0_0_1"/>
    <dbReference type="InParanoid" id="H6QUP2"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004342">
        <property type="entry name" value="EXS_C"/>
    </dbReference>
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        <property type="entry name" value="EXS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="domain" description="EXS" evidence="6">
        <location>
            <begin position="10"/>
            <end position="177"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EHS64754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="195" mass="21964" checksum="44FBE983B2A1B77F" modified="2012-04-18" version="1">MNQQQQQEHFPQPFRILSLIQLSITSTAISLNYYYYHQQEQQQTAITNNNNKTITTATATTTSTHQQLYYGALSLLITILLGLLTNQLLIHLLLIPPAHAPLLASIPAFILLVHPSRLAPFSAHFRNGHRKLLKALRRILALERSPQRLTRFPDLIIADLLTSLARPFADLALLCATTLHLHPSLQSSLSIFFNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUP3</accession>
    <name>H6QUP3_PUCGT</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22474</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178348">
        <property type="protein sequence ID" value="EHS64755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888763.1">
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    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUP3"/>
    <dbReference type="GeneID" id="13541631"/>
    <dbReference type="KEGG" id="pgr:PGTG_22474"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22474"/>
    <dbReference type="HOGENOM" id="CLU_539827_0_0_1"/>
    <dbReference type="InParanoid" id="H6QUP3"/>
    <dbReference type="OrthoDB" id="10352185at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="450" mass="50890" checksum="F52C82BF97E39743" modified="2012-04-18" version="1">MSATPTHTRSEETPEPPSSEPNEHNRIMGNLPMTRVQVANYGPTPISPLPTPYGIVEHYRPYDRAQRQQWYPRRAASTRGRRRWSTPPPTRPPSQMLTRHIPTPHNVFDTEDDDTDGVSPTNPPGNVTPTAPRPVRISADDTIGHLPFNLVRQLGLDSVDVELADRLIDTSPEYRWPLSVLLMVSAQQGPVRPMAPRHRPRPTAEPRADPETFAFEDSFNHSVHPPEWDLGLLQSPNAHHPNPIGQPPNALDVLNLAMLPPGYTSSDPSSIAHLQRLFKENITEEKARLRRTIMVKISPYYSAHGPIPNLDDLMAYVWHKQRPDPDLQPAPLEPPPPGRQVDRVRIAHTRLHMLNYKYGWPVPSSSNWTTFDRDLQALAGRDSIYRQAHANAILLRDTLLFNGLNTLASIPEPDRRLPNEREIDHQIMMIEVGATLLDIGEPELGREHEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>H6QVH9</accession>
    <name>H6QVH9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22754</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178425">
        <property type="protein sequence ID" value="EHS62994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888483.1">
        <property type="nucleotide sequence ID" value="XM_003888434.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QVH9"/>
    <dbReference type="GeneID" id="13540977"/>
    <dbReference type="KEGG" id="pgr:PGTG_22754"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22754"/>
    <dbReference type="HOGENOM" id="CLU_204790_0_0_1"/>
    <dbReference type="InParanoid" id="H6QVH9"/>
    <dbReference type="OrthoDB" id="10311625at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="50" mass="5682" checksum="9AB8FA965127BD51" modified="2012-04-18" version="1">MHLWTEWWSPRNTKDRQVLVQTPAGATRGIHGKNRYRPTSLHSGGLAVTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3JPX5</accession>
    <name>E3JPX5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_00014</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74058.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307064.2">
        <property type="nucleotide sequence ID" value="XM_003307016.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JPX5"/>
    <dbReference type="GeneID" id="10528114"/>
    <dbReference type="KEGG" id="pgr:PGTG_00014"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00014"/>
    <dbReference type="HOGENOM" id="CLU_010596_0_0_1"/>
    <dbReference type="InParanoid" id="E3JPX5"/>
    <dbReference type="OrthoDB" id="2506706at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009816">
        <property type="entry name" value="SPATS2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15623:SF11">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED SERINE-RICH PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15623">
        <property type="entry name" value="SPERMATOGENESIS-ASSOCIATED SERINE-RICH PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171437" evidence="2">
        <location>
            <begin position="17"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="170"/>
            <end position="598"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="723"/>
            <end position="841"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="956"/>
            <end position="1057"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="193"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="211"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="288"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="356"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="378"/>
            <end position="392"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="400"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="414"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="426"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="452"/>
            <end position="472"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="495"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="533"/>
            <end position="565"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="582"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="732"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="743"/>
            <end position="765"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="772"/>
            <end position="836"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="965"/>
            <end position="975"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1009"/>
            <end position="1047"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP74058.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1057" mass="109744" checksum="BB37FCDA1EA36D3C" modified="2012-04-18" version="2">MKNLFFLLSCLATFDARIIIDETVYAPGRLARRRLNMEHQSAVNKLSGLKDCPGNVCGTFAGQAIAPLLAASDQCAQQDMADKIIDAAKDPSSGVTSKETREEMIKIAIEYRQAEKNTHPIYPQLIPRNSVYCQKAPKNSERKGLVQKQDPANGPLLFFDHLVTTNGSTVLLDSQPNTRPFGEKAGTKPGLGASPTDPKTTSSTDADPSGNVKQSDTGSGGSTNTAPSLKTTSGDASPKNSETGTGSSTNPKSTAGDADPQKSETDTGSSTNPKSTTGDADPQKSETDTGSSTNPKSTSTSGDADPKKSTTSNGLSSEENEDDDSCGGSGDDQSDNKQEDNSSSNNLKDTTSGADGSNGGNGGDTGNTQDSASPSLKDATTTAGGNNGDTGTQNTEKTDSTSTGNDNSNVSDATGKGTESSTTSGGEDGGSGGDDTGNGEKQDSTSPSLKDTTTSATNNNGGTGGQNTVKTDSTSDKTTDTGNGSADNGDKKGGTLSNGSTGLKDTSLQPDNKPEGDADDSGCGGKEDDETDTSANKSSSTSSTDTNANPQGSKTTSSSLTTASTGDQTPSSPGSDPKLKDTSSSTSGNPTPNTGTSADAQKVVIAILGHQLDVTKIPNKDCHDYRIEFGSGFKGREAKDVAYQPLQPIFEHGAALNMAIITRSMCDSLHNKCGVSQSSPEESLFKDCVLFSDGIKRVVNNGTMADAWNFLWGFHTNYATQKATGEGTGGKGDGKPQIEKSDSNTSSSSPTSPSATQPPNNNSNKGAGGTNQNGNTNQMGMDQNMQQNMNQNQPQSTDPNMNQQGMNQNMNQQGMDQNMNQNMNPPSGAPAADTGPPADPKAIVDKFLGHQINVTKIPNKDCHDYRIEFGSGFKGREAKDVAYQPLQPIFGHGAALTMCDSLHNKCGVSQTSPEESLYTDCITFSDRIGKVVNNGSMADGWNLLWGFHTNYATQKATGEGTGGKGDGKPQIEKSDSNTSSSPTPPPATQPPNDNSNKGAGGGTQSGNTNQMGMDQSGNMQQNMNQNQPQSTDPNMSQQNMNQNMNQNPGGDQKQGGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>E3JRP1</accession>
    <name>E3JRP1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bis(5'-adenosyl)-triphosphatase</fullName>
            <ecNumber evidence="7">3.6.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_00158</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11 7">
            <text>P(1),P(3)-bis(5'-adenosyl) triphosphate + H2O = AMP + ADP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:13893"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="3.6.1.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <dbReference type="EC" id="3.6.1.29" evidence="7"/>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307208.1">
        <property type="nucleotide sequence ID" value="XM_003307160.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JRP1"/>
    <dbReference type="FunCoup" id="E3JRP1">
        <property type="interactions" value="72"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JRP1"/>
    <dbReference type="GeneID" id="10546431"/>
    <dbReference type="KEGG" id="pgr:PGTG_00158"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00158"/>
    <dbReference type="eggNOG" id="KOG3379">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056776_7_1_1"/>
    <dbReference type="InParanoid" id="E3JRP1"/>
    <dbReference type="OMA" id="DAIYGMM"/>
    <dbReference type="OrthoDB" id="680339at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047710">
        <property type="term" value="F:bis(5'-adenosyl)-triphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004081">
        <property type="term" value="F:bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01275">
        <property type="entry name" value="FHIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.428.10:FF:000011">
        <property type="entry name" value="Fragile histidine triad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051884">
        <property type="entry name" value="Bis(5'-adenosyl)-TPase_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039383">
        <property type="entry name" value="FHIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46243">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46243:SF1">
        <property type="entry name" value="BIS(5'-ADENOSYL)-TRIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="8">
        <location>
            <begin position="42"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="6">
        <location>
            <begin position="135"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="3">
        <location>
            <position position="137"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="67"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="130"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for induction of apoptosis" evidence="5">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR639383-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR639383-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR639383-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP74202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="205" mass="23010" checksum="DA85B1E47750587D" modified="2011-01-11" version="1">MLLRNILKPWNRSPFRPPSQTLSAGSKIIHSKIIHGSIRMSSSLKFSSFDVTNQVFFQTSHSFAIVNLKPIAPGHVLVIPKRTEAKRLADLSRDEVADLFTSVQRVGSVMESVHKASSLTVAIQDGPCAGQSVPHLHVHVIPRRPNDFVPNDKIYDHLNRFDGSCEKESDKHQVGQLIIDNEERAARTLEDMQEEAKSLSQHFQD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3JSE3</accession>
    <name>E3JSE3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01461</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP74868.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319287.2">
        <property type="nucleotide sequence ID" value="XM_003319239.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JSE3"/>
    <dbReference type="GeneID" id="10547065"/>
    <dbReference type="KEGG" id="pgr:PGTG_01461"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01461"/>
    <dbReference type="HOGENOM" id="CLU_047292_2_0_1"/>
    <dbReference type="InParanoid" id="E3JSE3"/>
    <dbReference type="OrthoDB" id="2506484at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
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        <location>
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            <end position="236"/>
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        </location>
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            <begin position="214"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="265"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP74868.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="325" mass="35707" checksum="EE268037E3880B75" modified="2012-04-18" version="2">MATQKKLSHPMLGRSISDIFEQLEQKINPGSTYGQLFYSTSVAFTDPQTQQEKNILVKLCGYGSAITALIENHVYLVSGKFIPRNIKTTPVFHYDSDTIINLGETAQFSQSMCDKPSVLGLDVVVSKKEVIDSDGDCASKTLHVILQHTHYDPVTKSQVQFKSLYHVGGRKNFANTFGLFQLGREVLISGNIVGYSEDQFMWIVNAISISITSGSQSTTTQSSRSTQKPELVSRRPGLISIEDQPEPAHIEPQTQSIPNPGLLTPPNTVSQEAEVQSNTPDAPESGNYYDSIVSTSGKKRTKKEILADAKRAKKNLSTATPYVGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3JT73</accession>
    <name>E3JT73_PUCGT</name>
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        <submittedName>
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        </submittedName>
    </protein>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="KEGG" id="pgr:PGTG_01945"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01945"/>
    <dbReference type="HOGENOM" id="CLU_372603_0_0_1"/>
    <dbReference type="InParanoid" id="E3JT73"/>
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    <proteinExistence type="predicted"/>
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    <keyword evidence="1" id="KW-0732">Signal</keyword>
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        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171509" evidence="1">
        <location>
            <begin position="22"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="746" mass="85031" checksum="76EFC4CBA82A1D6D" modified="2011-01-11" version="1">MKNSSAQHLLAVCLLVRPILAGQLFTEAGPTLAALDLSKQDPSSSWNGGAPATEAVLGISRPFNILVNELDETRQPRRVRQKLKHHPPEPFTICSPAQHIHRRLKDFDLNHSPPPSPPEQVPIEYLPAEELPLEQGSVADVSAEKAPVEQVKVQHLAEHDPVGNIHEQVPIGNIHEQVPVGYIHEQVPAEQITEQQVPLEQAAQLHPKLDLDLLDMNEPPPKEFSLMISITHWAVVHPVLFIKYHTLFKEQVDFYTSKYAGKLLTKPGETFKDLPIYILRHMEPESDSSRWMILPKIIRLPKQRDPAASQTVQAAQPELKGRIQIPFMINQLLGSLIKGILFAHGAFLREIQCPVEEEIRRHDSLMNWLNELIFSTKNHPPICGEISPADWARVEGRGYTPAQRRLIYYLQGRPEGENLYLDYMALSLIGIWYKRDAPEEWLRHFQGSEYRFWMRMEDSITEVKRVRNWKAGKWIDQGLGEQKPDSKLGNFDLVSLRQVVKLDGSPHSKDKRYSWKVIEHFEPSEVEARVIRGHQRVTELVDLAIGRPMKAIEDARAALQLGSEKSTGQPVWAVRVIGLKGRPYPARGLKRKLIQLFGALRHYHQQLLKFFEEYEIPTIPNAHEAFLDWFTETLRNPPEPTALPLFGYVSEQGGELDATRFNPIQHLVLLFITTHSKALDGTTDVVALLGYWYKNLHPAYWAARFRSEFCFGQAVLLTQMPRSRADTVVRAIGNTRKQLQTGHTIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3JU12</accession>
    <name>E3JU12_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00868</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="GeneID" id="10543253"/>
    <dbReference type="KEGG" id="pgr:PGTG_00868"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00868"/>
    <dbReference type="HOGENOM" id="CLU_366867_0_0_1"/>
    <dbReference type="InParanoid" id="E3JU12"/>
    <dbReference type="OrthoDB" id="2501576at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0017057">
        <property type="term" value="F:6-phosphogluconolactonase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0009051">
        <property type="term" value="P:pentose-phosphate shunt, oxidative branch"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <location>
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            <end position="218"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="447"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="576"/>
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        </location>
    </feature>
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        <location>
            <begin position="609"/>
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        </location>
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            <begin position="194"/>
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        </location>
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        <location>
            <begin position="450"/>
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        </location>
    </feature>
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        <location>
            <begin position="489"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="509"/>
            <end position="525"/>
        </location>
    </feature>
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        <location>
            <begin position="526"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="544"/>
            <end position="553"/>
        </location>
    </feature>
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        <location>
            <begin position="628"/>
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        </location>
    </feature>
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        <location>
            <begin position="649"/>
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        </location>
    </feature>
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    </feature>
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        <location>
            <begin position="686"/>
            <end position="696"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75537.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="760" mass="82369" checksum="61D4FCB83D18784B" modified="2012-04-18" version="2">MDRISASERLHPTSEKRHSGYSILVVPPCFHPVGNITSSSSEKHSTLFPIHPGGIIIPLQSTLKNQISEVARYFTLPSTVGLSLHANVSIHGSLQSTRPAGQSGSDCPLPLISAASWNSLFFGYLGHDDIPKPQQTTWMGPSRPSGIGVLVRLEFRIDYHTASWYEAWLMATLCRKVQAPRDLDESVEEIRRQSSMAASSSSSESSQSTAPTSLNPSPVQFGLMKITGTLDAITPSSTPRDGSTRAIRSRSIHLLSQARQYRRNHPTEPQWVSKTILQPVRKNRNSSQDFDLFMTPPSSASNSVSENSVPKIEYQEAHVASREERALSSVMSTNETNSEDSDSTHRAIDKKFKISCESSSTDQVYNHPNISDDYFQSRISEPGKSVMMETAASEGSSIDREGTQVSAAELAANQTAPVTPDDKHDKRSSGVNIISDWKNFLSNMSPASASTSGVVESDGSSSENRSRRVPQRLNLEPAVEISNAAPVISPSSNTPSPVGSTFAMNKTPENLHVEQKQTQNRDRNSDVSSEGTSASGSRSPRPFLNSSPSSSIWKLSERGRKPWSLSKIVENIRSDSVKKKASHRGHPLRTGSYSSNDLLAISNSSDSLIKAGKHSSSSLDGMRKYHESPGSSIAQGSSHSSNSKEKLSVTASDSSLDQTAARRAVPMRVANQSNPGLGSESNRSSKVMESETEKAHPGTRGSSQWSQSAANCFPTFLPLSLNRMSSVSSLSSVQPVIHKARKVEIVNNSTSRLTTLKPPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3JVA3</accession>
    <name>E3JVA3_PUCGT</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_01309</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="AlphaFoldDB" id="E3JVA3"/>
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    <dbReference type="KEGG" id="pgr:PGTG_01309"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01309"/>
    <dbReference type="HOGENOM" id="CLU_1435074_0_0_1"/>
    <dbReference type="InParanoid" id="E3JVA3"/>
    <dbReference type="OrthoDB" id="2504456at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
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        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
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    </feature>
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    </feature>
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    </feature>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
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            <begin position="177"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP75978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="189" mass="21109" checksum="5922F97713FE5C06" modified="2011-01-11" version="1">MSARILSKRGGAGAGDPNHPALKADPNATYKPFPPESSYLPGLGRSENSGASRGLKIILALALIFGLLSFLLVGWTLFRYCKRKRIANIRKREASVGNRPLFSRRSRGPGWRELPPDHDHDDADADRDGGGQDQIYGHEVFEMSREFNQSNYDLPFSSKHLQFEDEHEEDEDNFINSPVSSRHSSSPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
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        <lineage>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Puccinia</taxon>
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    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
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        <property type="nucleotide sequence ID" value="XM_003320354.2"/>
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    <dbReference type="AlphaFoldDB" id="E3JVA8"/>
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    <dbReference type="KEGG" id="pgr:PGTG_01314"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01314"/>
    <dbReference type="HOGENOM" id="CLU_1310657_0_0_1"/>
    <dbReference type="InParanoid" id="E3JVA8"/>
    <dbReference type="OrthoDB" id="2502209at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="185"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="197"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75983.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="210" mass="23808" checksum="67DCF8E87437E464" modified="2012-04-18" version="2">MDSFADGSKSILQDDTQPYPHPNNRQRHLEALDRPTKRRRSISPLSDKLSRCKKPTLIHHHLQTALTKTKKEEHSLSPSCKSTPTISAAVDSINERATRLGLENAYPEDVEMDFCPISEGEEADDEETSYERERVPIHHHSTPNITTTTSSTSSILPLHKPHPLAYQQHLALISSTRSKKLSNSNRILNSQKLSVGHQDEHESRKENNHF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>E3JW48</accession>
    <name>E3JW48_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyclin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_02714</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the cyclin family.</text>
    </comment>
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        <property type="protein sequence ID" value="EFP76273.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003320644.2"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02714"/>
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    <dbReference type="InParanoid" id="E3JW48"/>
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    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="term" value="C:transcription factor TFIIK complex"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="term" value="F:cyclin-dependent protein serine/threonine kinase regulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd20524">
        <property type="entry name" value="CYCLIN_CCNH_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20525">
        <property type="entry name" value="CYCLIN_CCNH_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000095">
        <property type="entry name" value="Cyclin Ccl1, putative (AFU_orthologue AFUA_5G07030)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043198">
        <property type="entry name" value="Cyclin/Ssn8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031658">
        <property type="entry name" value="Cyclin_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006671">
        <property type="entry name" value="Cyclin_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10026">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16899">
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00134">
        <property type="entry name" value="Cyclin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0195">Cyclin</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cyclin-like" evidence="4">
        <location>
            <begin position="67"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU000383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP76273.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="334" mass="37437" checksum="CFF11BFC899FB424" modified="2012-04-18" version="2">MGAAHPDIRSDSDIRSPFQRNRTSASTSASAGGYPLALAAGDEPTKPSQIEYLTFQDELELVTFQLSKISQLCRALIFNFSETVEATVISYLKCFYLRNTCMDYHPENIMLTCLFLATKTENTSISIDSFASRIPKTTNGDVLALEFLVTQLLKFQFKVHHAHLAARGIYLDLQACPGVGPLGLEQLSQAWPEVCALLRTGRLTDAEFLWTPSQIACAAWWVADRSATEAWLKAKADRLQPSSNQSSTYIPSSLGFDPLTQYLQTLSELLIQAQRKPIDKDRVTAVDRRLRFCRNPEKDPNSALFKIKQAELEHAHLAKHHQKYPPNSNQDPFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWB0</accession>
    <name>E3JWB0_PUCGT</name>
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_02776</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003320706.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JWB0"/>
    <dbReference type="GeneID" id="10534227"/>
    <dbReference type="KEGG" id="pgr:PGTG_02776"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02776"/>
    <dbReference type="HOGENOM" id="CLU_051119_2_1_1"/>
    <dbReference type="InParanoid" id="E3JWB0"/>
    <dbReference type="OrthoDB" id="2498930at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027532">
        <property type="entry name" value="Mdm12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204:SF1">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="158"/>
            <end position="185"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP76335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="199" mass="22831" checksum="AE9E2447F32C6B40" modified="2011-01-11" version="1">MATEVVRESKILLTPDNYALWLLPMKAKLHKAKSLTIVTGEHTCPDPKEDKENTRLYVKLNEDAYAEIVQHHNQEILAYTNISMNFETEAFESVLKKLEDYASQNQLNEVKKAVITPQTTLYTQSSDPKVVCPHCKRGFRACSHCFKSGHTEANCFQKHPDKRAPKSVASTSKQHSAHLTRYTPEDEEYLQQKYPDLHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWR8</accession>
    <name>E3JWR8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF6818 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_02934</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320912.1">
        <property type="nucleotide sequence ID" value="XM_003320864.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JWR8"/>
    <dbReference type="GeneID" id="10528266"/>
    <dbReference type="KEGG" id="pgr:PGTG_02934"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02934"/>
    <dbReference type="HOGENOM" id="CLU_076951_0_0_1"/>
    <dbReference type="InParanoid" id="E3JWR8"/>
    <dbReference type="OrthoDB" id="2499380at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049203">
        <property type="entry name" value="DUF6818"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34409:SF1">
        <property type="entry name" value="MYB-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34409">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20681">
        <property type="entry name" value="DUF6818"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6818" evidence="2">
        <location>
            <begin position="87"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="182"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP76493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="314" mass="34326" checksum="EBA50D82C762176F" modified="2011-01-11" version="1">MVSDPQIQASQGNQNPPNPNQTADVDTQTQTNKSGSSRPNKGGLKKNKKNNDKLTTGKKTGGQPPGSLGYNVAECMLLVEAVKSVLPLGNQDWRSVADQYNTKVLLLNRPKREADNLKQKFKGLAQSQKPTGNATCPEHICEAKRVDALIHEKANECALGSLSSGDERYLICADVLSSNDDDVDVNETTGKLESSQQESTGTLLSGWSQTQSANQDLGDSPNPSQTHSMRRQTYQSPLVHWTHSQASSARPSPMTSVNRRSRGQPGDQLESRLNSFLDMDARKEQDRKQSLASFYSAQLMKANATIRDLQSELA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWS6</accession>
    <name>E3JWS6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Prephenate/arogenate dehydrogenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_02942</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76501.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320920.2">
        <property type="nucleotide sequence ID" value="XM_003320872.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JWS6"/>
    <dbReference type="FunCoup" id="E3JWS6">
        <property type="interactions" value="96"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JWS6"/>
    <dbReference type="GeneID" id="10528274"/>
    <dbReference type="KEGG" id="pgr:PGTG_02942"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02942"/>
    <dbReference type="eggNOG" id="KOG2380">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031403_1_0_1"/>
    <dbReference type="InParanoid" id="E3JWS6"/>
    <dbReference type="OrthoDB" id="5399569at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008977">
        <property type="term" value="F:prephenate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004665">
        <property type="term" value="F:prephenate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3660.10:FF:000006">
        <property type="entry name" value="Prephenate dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000339">
        <property type="entry name" value="Prephenate dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3660.10">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal like domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046825">
        <property type="entry name" value="PDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050812">
        <property type="entry name" value="Preph/Arog_dehydrog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003099">
        <property type="entry name" value="Prephen_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21363">
        <property type="entry name" value="PREPHENATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21363:SF0">
        <property type="entry name" value="PREPHENATE DEHYDROGENASE [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20463">
        <property type="entry name" value="PDH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51176">
        <property type="entry name" value="PDH_ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prephenate/arogenate dehydrogenase" evidence="2">
        <location>
            <begin position="7"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP76501.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="438" mass="48697" checksum="12680843A10F8C11" modified="2012-04-18" version="2">MEPSQDAVVGLIGMGEMGRMYADRLAAAGNCQLLVCDRPEKFDQLRDDYKDRPNVTVVANGHLVSRASDLIIYSVDSEHIEMIVEQYGPSTKLGAIVAAQTSVKAPEQAAFEKHLPSDTRVCSIHSLHGPSIPPDGQALVLIPFRCSVAQKQWVKDILAPLRSRYVELSYEEHDVVMANTQACTHAAFLSMGTAWHATGRFPWDSGRYIGGIEVAKINITLRIYSSKWHVYAGLAIMNPIARIQIDQFARSVTELFVLMVSDDEEGLRKRVHAGRDHVFGPPSSPRSSTPPMFLSEEVFSNFAIGEPAAGPSPPNSHLTILAIVDCWHQLGLNPFLHLDIAATPVFRLWFGLIHHLFDSPERLDKAIHAAVHNLDYRADDCEFVLAARGWSQCISYGDFKLYQARFQQTAEFFKPRIEEGKDKGSAMMKHILEVCSSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXB3</accession>
    <name>E3JXB3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Copper transport protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_02149</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Membrane</location>
            <topology evidence="4">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the copper transporter (Ctr) (TC 1.A.56) family. SLC31A subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321107.1">
        <property type="nucleotide sequence ID" value="XM_003321059.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXB3"/>
    <dbReference type="FunCoup" id="E3JXB3">
        <property type="interactions" value="140"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JXB3"/>
    <dbReference type="GeneID" id="10528627"/>
    <dbReference type="KEGG" id="pgr:PGTG_02149"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02149"/>
    <dbReference type="eggNOG" id="KOG3386">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_924815_0_0_1"/>
    <dbReference type="InParanoid" id="E3JXB3"/>
    <dbReference type="OMA" id="AKTVACH"/>
    <dbReference type="OrthoDB" id="161814at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005375">
        <property type="term" value="F:copper ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007274">
        <property type="entry name" value="Cop_transporter"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483:SF115">
        <property type="entry name" value="COPPER TRANSPORT PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12483">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 31 COPPER TRANSPORTERS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04145">
        <property type="entry name" value="Ctr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0186">Copper</keyword>
    <keyword evidence="4" id="KW-0187">Copper transport</keyword>
    <keyword evidence="4" id="KW-0406">Ion transport</keyword>
    <keyword evidence="3 4" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2 4" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="147"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="246"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="4">
        <location>
            <begin position="270"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="39"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="8"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="39"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP76688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="301" mass="32997" checksum="CB6D19E0B8324247" modified="2011-01-11" version="1">MSSLPSQATTTTTTTRRAAVASRLKPSIAQSIIRPQNLSTNIFTSSTNKKQAKTNDDQENNKQPTPSANPAAAFSSSSQFPFSFPHLAGLGLAMASNTLNSNDHHHHHHHNMEHSSPALEPEVMNHCSMNMVFNSQPIGTCIVFRQFYVSGTMALLFYLSLLFVIAVGYEYLRLASNRFERMIQVKLSGGASKRALSPPRRVSSNENLIDNPAASLVDGSNLSLINPKLASWGQISVPRSIQLTRSLFYVSNVALSFFLMLVVMTYNAQIILAVLAGAFVGHFVFHREISFGQPDKGMACH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXH6</accession>
    <name>E3JXH6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF7729 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_02212</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321170.1">
        <property type="nucleotide sequence ID" value="XM_003321122.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXH6"/>
    <dbReference type="GeneID" id="10528772"/>
    <dbReference type="KEGG" id="pgr:PGTG_02212"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02212"/>
    <dbReference type="HOGENOM" id="CLU_712023_0_0_1"/>
    <dbReference type="InParanoid" id="E3JXH6"/>
    <dbReference type="OMA" id="FYDAICA"/>
    <dbReference type="OrthoDB" id="2536450at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056146">
        <property type="entry name" value="DUF7729"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34862">
        <property type="entry name" value="SPARK DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34862:SF1">
        <property type="entry name" value="SPARK DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24855">
        <property type="entry name" value="DUF7729"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172218" description="DUF7729 domain-containing protein" evidence="2">
        <location>
            <begin position="23"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7729" evidence="3">
        <location>
            <begin position="97"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="301"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="311"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF24855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP76751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="388" mass="39769" checksum="71D2A99B171A401F" modified="2011-01-11" version="1">MSFSLPLVSILMSWISYQSVNAHPFGMFPSPGGGMSGMGGGMPGMGGGMPGMGGGFPGMGGGFPGMGGGFPGMGSASTPGATAEDQSGSSSIKTELSESCKKAAHTLSEGEVATCANIPALIAISKAEGSIVPAINTWITGACSAVPCRQEALNSAAEKFKKECQKDLDDGSAPAIGIYSHLLHYKEARNTVCTQSKSDSKFCIPQLMEGLEEKAGEKFTNAGIKASMAGKFTKAALAFMRVPKETFCTPCSHALATQSAIMVEAISKDPRIKFEHNSTPQMQKASEICGASFDDHKIPDSVQIAAPGTGKAEDGGAKDKADGPPKPEETEAGSETKPEDSGAPAEKPSDASAEAEKPKDGQTTPEASKGSAEGSEKPKDQDSGTQEA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3JYB7</accession>
    <name>E3JYB7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_02998</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321461.1">
        <property type="nucleotide sequence ID" value="XM_003321413.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JYB7"/>
    <dbReference type="GeneID" id="10529383"/>
    <dbReference type="KEGG" id="pgr:PGTG_02998"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02998"/>
    <dbReference type="HOGENOM" id="CLU_1661675_0_0_1"/>
    <dbReference type="InParanoid" id="E3JYB7"/>
    <dbReference type="OrthoDB" id="2495064at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP77042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="159" mass="18550" checksum="030A5E9DC500BD1D" modified="2011-01-11" version="1">MPQNAGKPRSIRLNRLSNQVFVAQIREPHQRAGTCEVSHTIMVPTGEDCLGYGIGACVAREGVYDVQIIIFPSQRRFLDQERPMRPIIRLGLAATPDGTNDFRRRVLNTKHMLWRRSIDDPDWCCRDWVLCILYAELDHHTFPHDRLWKVIRHLENPQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3JYV5</accession>
    <name>E3JYV5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03186</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77230.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321649.2">
        <property type="nucleotide sequence ID" value="XM_003321601.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JYV5"/>
    <dbReference type="GeneID" id="10537606"/>
    <dbReference type="KEGG" id="pgr:PGTG_03186"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03186"/>
    <dbReference type="HOGENOM" id="CLU_2373836_0_0_1"/>
    <dbReference type="InParanoid" id="E3JYV5"/>
    <dbReference type="OrthoDB" id="10344962at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="64"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77230.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="95" mass="10598" checksum="C6BA9A54201FA4C6" modified="2012-04-18" version="2">MEPISAHAPLDENASKITLPLSGFHFEDYNPFGLAEFAADDEWFKLGDVPLYFESFPSLPAEARAKEPKSLDNQHFTQDINSPLKHGTRNPGVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZ27</accession>
    <name>E3JZ27_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03258</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77302.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321721.2">
        <property type="nucleotide sequence ID" value="XM_003321673.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZ27"/>
    <dbReference type="GeneID" id="10537848"/>
    <dbReference type="KEGG" id="pgr:PGTG_03258"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03258"/>
    <dbReference type="HOGENOM" id="CLU_059061_0_0_1"/>
    <dbReference type="InParanoid" id="E3JZ27"/>
    <dbReference type="OrthoDB" id="2502132at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <location>
            <begin position="10"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77302.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="426" mass="49634" checksum="2D9137A8A052C3FF" modified="2012-04-18" version="2">MVEPKVIYTAQESKQQAMDDPEQWMDEEDEEDEAEQLELRNKLRSARWYSLAPTREQALITGAFRKLLPKISGYVDRFRITNSKLKMSPDFSSHCHRIKEILLPCMLDNLFRLCALLFSPAHFKREVLPEFQAGLYALKGIEEAVDQISAAMISFWLPEDTTTNSHPTELPDIKHYRCELTQSKTADIMNKLTIILDLYGSMLGVSIGLCDIEVVDETWMCMVDGTRLMRDGVNQLIEWFRLSDHGVLQDQWRTMAEEAEELLQHLAELPSLNSSIQIQRDSIALIKVSRLFFKKISQVTKSEFHPIWRMTPIQLAHFMDSTRFFPSVLEAFVYSIEHEPNPHDHNIEMEPTNDLVKYFEEPIRILNDYLESAHQGSNPTPTQVQSHHEFCEWHTTWNKQFTLAVDRFQATYREVHSGLQDREDQV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZB0</accession>
    <name>E3JZB0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF7872 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_03341</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77385.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321804.2">
        <property type="nucleotide sequence ID" value="XM_003321756.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZB0"/>
    <dbReference type="GeneID" id="10538095"/>
    <dbReference type="KEGG" id="pgr:PGTG_03341"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03341"/>
    <dbReference type="HOGENOM" id="CLU_030195_1_0_1"/>
    <dbReference type="InParanoid" id="E3JZB0"/>
    <dbReference type="OrthoDB" id="2501761at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057194">
        <property type="entry name" value="DUF7872"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33339">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33339:SF1">
        <property type="entry name" value="LYSM DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25278">
        <property type="entry name" value="DUF7872"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173393" description="DUF7872 domain-containing protein" evidence="2">
        <location>
            <begin position="31"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="domain" description="DUF7872" evidence="3">
        <location>
            <begin position="252"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="46"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="55"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF25278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP77385.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="467" mass="51475" checksum="99C94A8F470CE8C2" modified="2012-04-18" version="2">MLATLHPGFWSKLSIFFALQVILIAQATESEQPSLEKRGFGFGPAAAAGAGSSSDKPHGPPKDPSDTLPEHCKLKPLTPQTWATLQLDNYLEKYPGGANMTLSQYAASQGVPNFQCGIGNTCDAGQPCYPVAPPDWYILFAVQQWNVKMNTMRSAIAYAMNFVKSTMATLFAALVPAIDTYQLDRMKLNFGVNGAMTMCSNTVLLDLFSLFHNFQDATGDAFNIINNIMGAAMSIAGGLIPSPDPPEGRQREGFNLWAHLEKQMTGYEELLTTKLSNETSIVETSGISTEKGIYGQLRNGNYIKPIQSIFLPIVEDDIKNVTIAISLVKILRTLDAFVTIGQEVCNKDGKDGAFEGDNVLSYCDRQGTMFNIIRVKEKKESMEFSNAWAIENRFGYSTQLLTNASWTCQQKYGGFEPFPYKNLTLPRDVLADCIINLPVCDCRDPKIKKQIRKHGVVEACRRAGLPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZQ7</accession>
    <name>E3JZQ7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_03488</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat RAPTOR family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77532.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321951.2">
        <property type="nucleotide sequence ID" value="XM_003321903.2"/>
    </dbReference>
    <dbReference type="FunCoup" id="E3JZQ7">
        <property type="interactions" value="342"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JZQ7"/>
    <dbReference type="GeneID" id="10538571"/>
    <dbReference type="KEGG" id="pgr:PGTG_03488"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03488"/>
    <dbReference type="eggNOG" id="KOG1517">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001136_2_0_1"/>
    <dbReference type="InParanoid" id="E3JZQ7"/>
    <dbReference type="OrthoDB" id="10262360at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031931">
        <property type="term" value="C:TORC1 complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071230">
        <property type="term" value="P:cellular response to amino acid stimulus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030307">
        <property type="term" value="P:positive regulation of cell growth"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010506">
        <property type="term" value="P:regulation of autophagy"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031929">
        <property type="term" value="P:TOR signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004083">
        <property type="entry name" value="Raptor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12848">
        <property type="entry name" value="REGULATORY-ASSOCIATED PROTEIN OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12848:SF16">
        <property type="entry name" value="REGULATORY-ASSOCIATED PROTEIN OF MTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01547">
        <property type="entry name" value="YEAST176DUF"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00678">
        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50082">
        <property type="entry name" value="WD_REPEATS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50294">
        <property type="entry name" value="WD_REPEATS_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 4" id="KW-0853">WD repeat</keyword>
    <feature type="repeat" description="WD" evidence="4">
        <location>
            <begin position="1430"/>
            <end position="1471"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="244"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="561"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="813"/>
            <end position="835"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="915"/>
            <end position="1022"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1073"/>
            <end position="1128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="244"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="272"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="576"/>
            <end position="592"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="937"/>
            <end position="947"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="994"/>
            <end position="1017"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="1082"/>
            <end position="1091"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP77532.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1573" mass="175808" checksum="F168DE4CF7F8FB31" modified="2012-04-18" version="2">MLPRDPKLPADVFTSCLTSPIEMALRFYVLRNNSQRSQWTSTAEFFQDLGKLDKVPGRMEMRRTPLGELTWIFTSIADAIAWNHLDRQTFTKIFRGDLVLAGTFRGFLLAERVMKAYGCTPMSTPKLPATHHSELWHTWDLEVDMCLSQLPGIWKSEEARAVYEKNKQNYPPNSLPPQIVPYMPSTFFSQQLTAFEVWLKFVVAENQDESGEKDEDLMKRRESLQWSCNNWSDVNINQVEITDFSCSNPNSQTSPKDKRASVKVNGKTPHLAGKSSGSHASPASGAPKAVKIPEQQPAERPIRYKLPTHIKHFPLNSRKGDDIYPPSPPQLPIVLQVLLSPVHRLRALILLCRLMDLGPWAVHLSLSIGIFQYVLKLLQAPAADLKPVLIFIWARILTVYPEGRQDLMRVASRPHARPEGPIEYFIKIMAPNSMELPIVNVVDHKAMCAFILSITCQNSRGNSLALMQFGVLDIIIYRIKELVPWQRQWYLILLAHMWEESDLVKGRAIKMGINTLLSDLLTDKVPEVRTAALYAFGTLIGVSTNPKQLVIEDQFPIIPGQTEERQPESSSSRPQNKSNPSVYQTNKLSSLSSSNPDLTVLGYQEQVSIEVGSAMACCHCSTDGSPMFRQELVVVLSALVDQHLGHFIVAAFEFIKQQEQEKSMKINKLIQKYQADRQQKSSSKQTALGADESHMMTDRTAKLEKLINKLKADEEVHPKESLRAYLWMEYTSIYIVLLDLSLDPSEQVARPAKVVVDYIHSRLLDSVLGKLLELGDLSYGEAPGGGVDGTGQLSETPLGRLGNHSHSNLIASSFASTSSNPTGREPKSSNQSLVNRSSTSVFHSLSTVNNLVPNFVKSSGWKTPFNSLSRSPEQAVFNSNDPVASTLTQSPQDLNKSASGLRKSASVNPIINVELDSPQSSQPSKMPLNHNGFSHASSTSSSQTQCSTEDDRHLQGLSTNQSIESIIAHTRASDHTRRQRNRSYPLQPAFPSAASPSYQPGKMNASTSTTSPVANPSHQEDGEEENYLYYLGLHNLEKLKAELNSRPEPSERQTMLPLMSDYYRLSSHIFLKPKMTSSGGESESESSDSDNNDDHDNDQPQVFNEKAEYTEQRPPTHQSHHPRAPNHQNTVVDDAALSHISHPHHFHPPHHHHIDAGSLENIKDSWRKQRNLRVILDGPTLKSNPGPNNWDHLVCSFNHSKPINNLLMHQFEDQLITSDSSGYITVYDWRSKSILNRFQPSDDPLDRITSMQLINQENVALLLASTPDGNVRIFRDYERPGKLAMVTAFKGLPNNEPSSLGESGSVTDWQQSTGLLLVGGNSRDIKVWNSRRERCVEDIKTRSNSCLTSLSSDHHSGWMIAAGFGDGSVRMFDRRKPPKNSMVKVFRSVHSSWCSKIKLQAGHRRELFSADSTGLIAQWDLRFDSPLRTFLAHEEGMPAFDLHDHAPILASGSMNGSVKIWNLDNGNDENDEEFTVERAPRVLFNLRNLYESTDAQDPQGSNTLSGFPSTSITSPLMGDYGHRLRGIGIGNPLRDALSSLQPITGLCFHPHRAMISITDGNGKLNIYSTDIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3K0A2</accession>
    <name>E3K0A2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_03683</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322146.1">
        <property type="nucleotide sequence ID" value="XM_003322098.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K0A2"/>
    <dbReference type="GeneID" id="10539099"/>
    <dbReference type="KEGG" id="pgr:PGTG_03683"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03683"/>
    <dbReference type="HOGENOM" id="CLU_2185270_0_0_1"/>
    <dbReference type="InParanoid" id="E3K0A2"/>
    <dbReference type="OrthoDB" id="10298044at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP77727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="109" mass="12029" checksum="B59B8B02C83E4CD4" modified="2011-01-11" version="1">MATATTTAVLPSLRVRQDHQDLPASISFVSDHDGLAPTSSGTNYIETAGDFVKNQSFGRNLFTVGNASNKNSDDSFPLNLDFIPMKFSNHTEEIFNDLHQPITRIQTFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3K1N7</accession>
    <name>E3K1N7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PPPDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_04168</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeSI family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP78212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322631.1">
        <property type="nucleotide sequence ID" value="XM_003322583.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K1N7"/>
    <dbReference type="STRING" id="418459.E3K1N7"/>
    <dbReference type="GeneID" id="10542003"/>
    <dbReference type="KEGG" id="pgr:PGTG_04168"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04168"/>
    <dbReference type="eggNOG" id="KOG0324">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG0907">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_033441_0_0_1"/>
    <dbReference type="InParanoid" id="E3K1N7"/>
    <dbReference type="OMA" id="VTLQMAC"/>
    <dbReference type="OrthoDB" id="2499654at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006611">
        <property type="term" value="P:protein export from nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032434">
        <property type="term" value="P:regulation of proteasomal ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02947">
        <property type="entry name" value="TRX_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1720.30">
        <property type="entry name" value="PPPDE domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008580">
        <property type="entry name" value="PPPDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042266">
        <property type="entry name" value="PPPDE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013535">
        <property type="entry name" value="PUL_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12378:SF7">
        <property type="entry name" value="DESUMOYLATING ISOPEPTIDASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05903">
        <property type="entry name" value="Peptidase_C97"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08324">
        <property type="entry name" value="PUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00085">
        <property type="entry name" value="Thioredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01179">
        <property type="entry name" value="DUF862"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51858">
        <property type="entry name" value="PPPDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51396">
        <property type="entry name" value="PUL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPPDE" evidence="6">
        <location>
            <begin position="12"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Thioredoxin" evidence="4">
        <location>
            <begin position="159"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="domain" description="PUL" evidence="5">
        <location>
            <begin position="334"/>
            <end position="634"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP78212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="637" mass="70912" checksum="33110C0FFC9AC9D7" modified="2011-01-11" version="1">MGSAQDQSDSTHSVKLYAYDLSNGLARSMSLGWTGRQFEAIWHTSVVYDDQVEIFFGQGITTCAPGQSHHGKPLKIIDLGSTMIDPQTLMEYIDGLRQTWTADVYHLLEKNCNNFSNELVGFLNGASVPDYILNLPQEFLATPLGASMRPMIEQMFRGSNPAPLAPQLSTTSSAPNPAHLLADIASSAYSAPTDTPLVSCSAVSAFDRELSQHYCVVVNFTNERGCPPCRVIAPIYEELARKYHSTPAGSNVHHGRQPKVKDIKFIKVDTATSMELSQRYQIRATPTFKFFINKKEVGEMKGANQTELETQINLMVYTAYPPHPHLKLRLPKLKALSPSPILYPQVFNFEKALNKLLDSFPSDSNSPREVIKQTFEWVVIPFLNNKTDLNSPDRFLQWARATTQVLDSLGSSDSFPALDFLRLAVLREEYVDQLFICPPDSNPLYEAIRIGNSQSLKGTQLARPFSLTLLRLLSNGLGAPKLAEQMIERETQNEGQFLRFVVGRLLDGDVDVQVLASGVFYGLVSRWASIRVDWQDTRPVDRAGSEEEWEVESMSALVESLDREAKKTEPNSEMIYRLIATIGKLIYLSESSSSLQTLLESLGFQSIIDGLMNNPGLLKANPAIKDLGKELESVMSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>E3K254</accession>
    <name>E3K254_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FCP1 homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_04379</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78423.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322842.2">
        <property type="nucleotide sequence ID" value="XM_003322794.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K254"/>
    <dbReference type="STRING" id="418459.E3K254"/>
    <dbReference type="GeneID" id="10536021"/>
    <dbReference type="KEGG" id="pgr:PGTG_04379"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04379"/>
    <dbReference type="eggNOG" id="KOG1605">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056219_0_0_1"/>
    <dbReference type="InParanoid" id="E3K254"/>
    <dbReference type="OrthoDB" id="277011at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07521">
        <property type="entry name" value="HAD_FCP1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1000:FF:000270">
        <property type="entry name" value="Nuclear envelope-endoplasmic reticulum network protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011948">
        <property type="entry name" value="Dullard_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004274">
        <property type="entry name" value="FCP1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050365">
        <property type="entry name" value="TIM50"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02251">
        <property type="entry name" value="HIF-SF_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12210">
        <property type="entry name" value="DULLARD PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03031">
        <property type="entry name" value="NIF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00577">
        <property type="entry name" value="CPDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50969">
        <property type="entry name" value="FCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FCP1 homology" evidence="2">
        <location>
            <begin position="261"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="117"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="205"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="146"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="205"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP78423.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="458" mass="50621" checksum="4D331A93F4FD544C" modified="2012-04-18" version="2">MNSLTWLLAPRRSPSKSQNLTHEPDQACEQSKTTTNPSGTLSHSSSSSSLSSASLQLGSGPRETEAELVDKMVHSIERQSGTIAANKLERKTSMGLDGELRFGLPGITERRRTLHLEPGPTKLPQSNHNTNNDPVDLINQSSTPLTPTPLSTPPPSSPSPIKLFSSTSSYNPYRHLIRLLPVRLGVKLYILIRKFISLFGLNLSSSSSSSTSDSPSPTTTTTTTTTTTEKPSETKPKSQLFKSMWSKLTPKNSTLKVNELLPRRNKTLVLDLDETLIHSTSRLGGIGGGKAWSNQSNTSAGLKVRVVEVVLDGRIVVYHVYKRPWVDFFLKTVSSWYTVVIFTASMREYADPVIDWLDQGRGLIEGRLFRESCTNIQGSYVKDLTVVERDLSKVCLVDNSPISYGLHQANGIPIEGWLNDPQDEGLLELLPMLDSLRFTKDVRRILGLKAFSIEPHKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2G3</accession>
    <name>E3K2G3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_04488</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78532.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322951.2">
        <property type="nucleotide sequence ID" value="XM_003322903.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2G3"/>
    <dbReference type="GeneID" id="10536296"/>
    <dbReference type="KEGG" id="pgr:PGTG_04488"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04488"/>
    <dbReference type="HOGENOM" id="CLU_038723_0_0_1"/>
    <dbReference type="InParanoid" id="E3K2G3"/>
    <dbReference type="OrthoDB" id="2501326at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090734">
        <property type="term" value="C:site of DNA damage"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016567">
        <property type="term" value="P:protein ubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031297">
        <property type="term" value="P:replication fork processing"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16448">
        <property type="entry name" value="RING-H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052639">
        <property type="entry name" value="TRAIP_ubiq-protein_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46569:SF1">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE RFWD3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46569">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE TRAIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="4">
        <location>
            <begin position="78"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="386"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="441"/>
            <end position="501"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="171"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="386"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP78532.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="530" mass="59917" checksum="29C9E0A173F369FE" modified="2012-04-18" version="2">MSSAIQSRPSWTGPLKVLMSIVYLRVSSPTIALTLHLPSEIRSISYKQQSRETHQYQHSILTASTRMSSSRIIVLPTCSICQDDEAGLDIVATICGHVFHLSCIRTWDESQFSRRHPTKCPSCNITLRRAASTSSAARFITLHSLTEREVDDQGSSQVIARTGDSSTDPRLSRCREELNSLKETLKEREQKIQQLTLEPVALRRERENLTATLRVLNHSKQELEDRVLKVSRDLRIERKERTDERLARSLDEKRIKTEMDKLNEKYRIAKNEGEQLHEQYVKKVSENEELQSFVSEFGQEKARFQATEVALRAQLQESIERFKSVKAANLVYKDKLEKKRNFFEDKAGMGDRSLTSSVRRCSKFYPDVSKSSIHPGGLMVLSSNQATNTTSAQPSKRANQPTSVQSPSTESSKRICRTKPPADHHQNHPSANVQIEIQSSSDPEDLTHHNHPSSSNIHSSSPPPNIPADQDLVMPSLFGRDLSTPARDQKNKLPLPFNHLRTNQASSSSAFKFSKPADKLLALGPKVRHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3K310</accession>
    <name>E3K310_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Casein kinase substrate phosphoprotein PP28 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_04823</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323286.1">
        <property type="nucleotide sequence ID" value="XM_003323238.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K310"/>
    <dbReference type="STRING" id="418459.E3K310"/>
    <dbReference type="GeneID" id="10536971"/>
    <dbReference type="KEGG" id="pgr:PGTG_04823"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04823"/>
    <dbReference type="eggNOG" id="KOG3375">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068528_0_0_1"/>
    <dbReference type="InParanoid" id="E3K310"/>
    <dbReference type="OMA" id="IDMESPR"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019380">
        <property type="entry name" value="Casein_kinase_sb_PP28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039876">
        <property type="entry name" value="HAP28"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22055">
        <property type="entry name" value="28 KDA HEAT- AND ACID-STABLE PHOSPHOPROTEIN PDGF-ASSOCIATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10252">
        <property type="entry name" value="PP28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Casein kinase substrate phosphoprotein PP28" evidence="2">
        <location>
            <begin position="121"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="59"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="94"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="106"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10252"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP78867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="216" mass="23533" checksum="D8B7C9E1EE3BDD1E" modified="2011-01-11" version="1">MVRNAGKFGKPKRGGKKQFTQNLAESDRAGMWDGEAKGSESEESSEESEDSEEEESGAEELNSKLASTSLGSSSKPQEDSSITQTRAERKAAKKQAAGKSTKTGNDGEDSDDDDQDLGLGNSNRVVKKSVKINEISNLDTGTLNRKEREAAEKKAAQERYWKLHQAGKTDEAKVDLARLAKIRQEREQAAAFRKAEAEAKAKEAADRAALSGRKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3K346</accession>
    <name>E3K346_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_04859</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78903.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323322.2">
        <property type="nucleotide sequence ID" value="XM_003323274.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K346"/>
    <dbReference type="GeneID" id="10537009"/>
    <dbReference type="KEGG" id="pgr:PGTG_04859"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04859"/>
    <dbReference type="HOGENOM" id="CLU_2942848_0_0_1"/>
    <dbReference type="InParanoid" id="E3K346"/>
    <dbReference type="OrthoDB" id="10465631at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP78903.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="60" mass="6760" checksum="C0442FD0375A556A" modified="2012-04-18" version="2">METPVQASSPRLMKRMYQHNPAEHLDPHLFTKNADSSLVKSCVKNDGETSKGYDGQEIQL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K5T1</accession>
    <name>E3K5T1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05823</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79502.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323921.2">
        <property type="nucleotide sequence ID" value="XM_003323873.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K5T1"/>
    <dbReference type="GeneID" id="10531330"/>
    <dbReference type="KEGG" id="pgr:PGTG_05823"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05823"/>
    <dbReference type="HOGENOM" id="CLU_032239_1_0_1"/>
    <dbReference type="InParanoid" id="E3K5T1"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="32"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79502.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="309" mass="34026" checksum="CBFBC702F44D0E7C" modified="2012-04-18" version="2">MSSGNSNTSSPVEIPAPRGYQALSNPTPANDDGIRYYPERGDWSPLFVPNAETPRAADIPVNPMSGPDPDPTGETVNAPVTSMADCDTTMEAAVLNNAPDFSRPPPIQTFPAPIPLPYGMELIQTGTHFLPQKEKWQSLYDPAGKFDLSTIPDEVPVPRGFIDPHPFCNMPVCCDPVFQNPPPGLVVPAVHPLMAANLPSHAPGAHPAPASTSTSDSSTYPPLSEFLHHGHKDVQDLLSKKNSPPDATCLYTEEDVMRIFEGIAPEFVWLCDDFPPHTLNEFDRRVMFFENMQWHFKCKKSTYLSTRNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3K6Y4</accession>
    <name>E3K6Y4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05298</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EFP80073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324492.1">
        <property type="nucleotide sequence ID" value="XM_003324444.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K6Y4"/>
    <dbReference type="GeneID" id="10534090"/>
    <dbReference type="KEGG" id="pgr:PGTG_05298"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05298"/>
    <dbReference type="HOGENOM" id="CLU_168454_0_0_1"/>
    <dbReference type="InParanoid" id="E3K6Y4"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="71"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP80073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="100" mass="11232" checksum="CA1126135C57F6DF" modified="2011-01-11" version="1">MLDQKLSHTPEFEIPPVPEAVSAPHSPHQTKTGHHVMAPTKDTGDLEEKPVVAPTEEPAKEVKPRKAVRKNSTEKQVRHSRRIFDQSHGEEGLLSKCQRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3K7B2</accession>
    <name>E3K7B2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_06217</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the major facilitator superfamily. Vesicular transporter family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80261.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324680.2">
        <property type="nucleotide sequence ID" value="XM_003324632.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K7B2"/>
    <dbReference type="STRING" id="418459.E3K7B2"/>
    <dbReference type="GeneID" id="10539265"/>
    <dbReference type="KEGG" id="pgr:PGTG_06217"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06217"/>
    <dbReference type="eggNOG" id="KOG3764">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_001265_51_2_1"/>
    <dbReference type="InParanoid" id="E3K7B2"/>
    <dbReference type="OrthoDB" id="440553at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17325">
        <property type="entry name" value="MFS_MdtG_SLC18_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050930">
        <property type="entry name" value="MFS_Vesicular_Transporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506">
        <property type="entry name" value="GH10249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23506:SF23">
        <property type="entry name" value="GH10249P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="68"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="101"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="132"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="157"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="183"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="300"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="340"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="368"/>
            <end position="384"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="390"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="430"/>
            <end position="452"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="464"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="9">
        <location>
            <begin position="30"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006829"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP80261.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="516" mass="54821" checksum="21A947F204510098" modified="2012-04-18" version="2">MADQKISKSWLPRCRCSNSIKKFTGSVTYITFTISFGLLVDMAAYGLMVPVLPFRLESIGYHNIPAKSSYLIAAFALGLIVSSVPIGIFGEVVKARKAPLLFCLVFLAASLILFWLSSSFVVLIIARLLQGVSGTGIWTLGLALICDVVPEERIGVVMGYVMIGWSIGAVGGPLAGGLLYDSLGYHSVFIFALILTGIDFILRLMVQDNQSSPTGEVINISEESGPKTGRTSLSDETARVIEPPTEIAALVDITAQKTEGIASQRKGKEINHAFLSQATYPSSSRPPTASRALLSLFSSARCWTLFSIIFVLGFCFGGLLDSGMTLLVHARYGLNSRGAALVFIGAVVPSFASSPLAGHFADKYGARWPVVICLILGTPFFGLISMEGSLSTFVIWTALTGLFVMGIAAPMMQDLAEVVKVTPGLGYAHVYGIFNMVYSTGSLVGPLCVGGILESCGVERGWKIVSLICAGLCAASILPAWLYIGNKGTISTNVVTPGLLPTNEKTPSDTNQNSPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3K7U0</accession>
    <name>E3K7U0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_06334</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80378.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324797.2">
        <property type="nucleotide sequence ID" value="XM_003324749.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K7U0"/>
    <dbReference type="GeneID" id="10539387"/>
    <dbReference type="KEGG" id="pgr:PGTG_06334"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06334"/>
    <dbReference type="eggNOG" id="ENOG502SCU9">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_026588_0_0_1"/>
    <dbReference type="InParanoid" id="E3K7U0"/>
    <dbReference type="OrthoDB" id="2138242at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="91"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="320"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="407"/>
            <end position="513"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="541"/>
            <end position="597"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="18"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="91"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="119"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="137"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="219"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="349"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="408"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="431"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="448"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="479"/>
            <end position="490"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP80378.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="655" mass="70540" checksum="A05B3399CD7ADDFC" modified="2012-04-18" version="2">MSIADERSLLKTTHHHLSSSSSSNHPSVSPETRTQHQKLSADSFTEPISPLVIGFNQPHEPTAIQQVANTIRLREEQKRLIEHRRNSFAGLNSFSFSNNNTRSPNPPSQNNNNRYSPRTVRTSQPASRASFSKVALSSSSSNNNNNNNNNHSNNNGSNTGHNGLMKLLSHPSLSNSTSSTSNQPSQQSLYDAPPPVVSLPSIDEAAARKPPSNPHRQALPTSTAPINQDTHPDPNTSPDMLDRSSSPTDSSTQMGPSSSVADRRGQVVREKVKNMALAPQAFRPQLLVQPSVKSAPIRSGFLQATAPNGEVVTTPLIARTNLAGSGGTGSTNPNHAPEPPQQPRFAKYNPNNPSSLNHSSRLNHSIGAGPSTAYPNLGASEGPYRQGIERRMTMHAGSLMQVPTLSHLLSGSNNPLSQSQPSPGRGRPSGKSANPAANHQTQSIPLGPGQPNPNPPYPPNHHHYHQSQSQQRAHHGTDGQRTATFQEQAPSQPPGSARLRGSIGGGGMMGGSTAAGIPSKQMFLQLFESFYDSLSDSKVLQNNLEEQIRRSAQLLHSLQQSATVFEAILDDRLAAVQTQSTRELQILENRIDRLEARLPLPNNLSNPNDEDELLPLDVLPDPADHKSPIIPTGSPKIPHSTVCSHSLPVSLLPLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBQ6</accession>
    <name>E3KBQ6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pali-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07964</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326134.1">
        <property type="nucleotide sequence ID" value="XM_003326086.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KBQ6"/>
    <dbReference type="GeneID" id="10533423"/>
    <dbReference type="KEGG" id="pgr:PGTG_07964"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07964"/>
    <dbReference type="HOGENOM" id="CLU_1205264_0_0_1"/>
    <dbReference type="InParanoid" id="E3KBQ6"/>
    <dbReference type="OrthoDB" id="3881at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035838">
        <property type="term" value="C:growing cell tip"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051380">
        <property type="entry name" value="pH-response_reg_palI/RIM9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28013:SF4">
        <property type="entry name" value="MARVEL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28013">
        <property type="entry name" value="PROTEIN DCV1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="99"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="20365" checksum="E17FC616F96A2CD3" modified="2011-01-11" version="1">MSAALTRDVLNLIPQSVAVFDRITVRIGLLGYCINDTCTQYHLGYRLPALLVGGPVGLDFLSRATTTALVINPIFTAFSAACLVAFLVSIFLKKPAIVFALMTLNTIIGIVCLIIDFTIFVRAYWLVRNSQGGIFGMVGVEYGSALWLMLAAVILTIFSTLFFAGTLHRGNDSRSNKGKMTSGRHNGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDD6</accession>
    <name>E3KDD6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_07542</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82145.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326564.2">
        <property type="nucleotide sequence ID" value="XM_003326516.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDD6"/>
    <dbReference type="GeneID" id="10534677"/>
    <dbReference type="KEGG" id="pgr:PGTG_07542"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07542"/>
    <dbReference type="HOGENOM" id="CLU_1540830_0_0_1"/>
    <dbReference type="InParanoid" id="E3KDD6"/>
    <dbReference type="OrthoDB" id="2510491at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP82145.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="195" mass="21473" checksum="ADE7C05BB48854CA" modified="2012-04-18" version="2">MQGGSIGEALDMIIYVVHPEYPLFVKRHKCTTSWPAKNIPQTSLLGGACRGHRLRTHRGWACVTYIVDPPLATKADAYHPRAAITLKLSPPSLYHLGEASCANQVPKPAPKVPQSFSCGARAPVCAWKVFVDPKAINPGQGDRVELSNAHNNHDGTWSCRGKYTMQSCCSLPVRDKSRFAIEIWQKECPDPANIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3KE40</accession>
    <name>E3KE40_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosome assembly protein 3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_08790</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Required for efficient biogenesis of the 60S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
            <location evidence="2">Nucleolus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RSA3 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327013.1">
        <property type="nucleotide sequence ID" value="XM_003326965.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KE40"/>
    <dbReference type="STRING" id="418459.E3KE40"/>
    <dbReference type="GeneID" id="10529977"/>
    <dbReference type="KEGG" id="pgr:PGTG_08790"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08790"/>
    <dbReference type="HOGENOM" id="CLU_943767_0_0_1"/>
    <dbReference type="InParanoid" id="E3KE40"/>
    <dbReference type="OMA" id="RFRKFWM"/>
    <dbReference type="OrthoDB" id="2504927at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030687">
        <property type="term" value="C:preribosome, large subunit precursor"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051898">
        <property type="entry name" value="Ribosome_Assembly_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028217">
        <property type="entry name" value="Rsa3_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28127">
        <property type="entry name" value="RIBOSOME ASSEMBLY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28127:SF1">
        <property type="entry name" value="RIBOSOME ASSEMBLY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14615">
        <property type="entry name" value="Rsa3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Ribosome-assembly protein 3 C-terminal" evidence="9">
        <location>
            <begin position="180"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="273"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="32"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="48"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF14615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP82594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="295" mass="31856" checksum="C0E85C2BC713B61C" modified="2011-01-11" version="1">MPPRPRKRVRRRRVSEKFDDSSDSSSSSSDSGQSNQSTPKNPNSVNKSSSDSHGSSSESESTDSSSSSSSSSSGSSINRPTAKRGRLAKKQNLQARALQPQDGGDPTNPQMMRRSPSPVEQSPPPPLPSSFFDPDLALPYLDSSPDFSLPAGFESKAPTRQVDGLLQSTRQQLEQDEERFRAWYMATIVDRFPNELDLLRSSDNNPQKTQSNLDLLVAALGSGVDIFDDNIILSSTSSSSPTVAANKAALDDHQLVLQSLDLAKQTLHLDPSICPQPVASPQSHEDVEMSDGADS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KEI8</accession>
    <name>E3KEI8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08751</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82555.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326974.2">
        <property type="nucleotide sequence ID" value="XM_003326926.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KEI8"/>
    <dbReference type="GeneID" id="10529937"/>
    <dbReference type="KEGG" id="pgr:PGTG_08751"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08751"/>
    <dbReference type="HOGENOM" id="CLU_2172288_0_0_1"/>
    <dbReference type="InParanoid" id="E3KEI8"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82555.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="110" mass="12222" checksum="44ED8FD322E9F206" modified="2012-04-18" version="2">MAGSENRRLLGPERSRRRTPEAKAETLTSREAQIRHLRNRRNLNLAKAGSGNRRPLDRTLKGRISMQASSVAWPPPLVPRPPTHPTPTGVAQGANVKRKRASSSKEEKNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3KEM6</accession>
    <name>E3KEM6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">26S proteasome complex subunit SEM1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_08936</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the DSS1/SEM1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327159.1">
        <property type="nucleotide sequence ID" value="XM_003327111.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KEM6"/>
    <dbReference type="FunCoup" id="E3KEM6">
        <property type="interactions" value="69"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KEM6"/>
    <dbReference type="GeneID" id="10530123"/>
    <dbReference type="KEGG" id="pgr:PGTG_08936"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08936"/>
    <dbReference type="eggNOG" id="ENOG502TC43">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_141774_0_0_1"/>
    <dbReference type="InParanoid" id="E3KEM6"/>
    <dbReference type="OMA" id="TTQHLWE"/>
    <dbReference type="OrthoDB" id="5586203at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000502">
        <property type="term" value="C:proteasome complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008541">
        <property type="term" value="C:proteasome regulatory particle, lid subcomplex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006406">
        <property type="term" value="P:mRNA export from nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043248">
        <property type="term" value="P:proteasome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13768">
        <property type="entry name" value="DSS1_Sem1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007834">
        <property type="entry name" value="DSS1_SEM1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16771">
        <property type="entry name" value="26 PROTEASOME COMPLEX SUBUNIT DSS1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16771:SF0">
        <property type="entry name" value="26S PROTEASOME COMPLEX SUBUNIT SEM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05160">
        <property type="entry name" value="DSS1_SEM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01385">
        <property type="entry name" value="DSS1_SEM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="2" id="KW-0647">Proteasome</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="3">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="55"/>
            <end position="68"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU369057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP82740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="128" mass="13882" checksum="556FA9A196B5A9F7" modified="2011-01-11" version="1">MASSTNSNPTNKTQNSKLASDLNKSSTPNPQQDTLGEDDEFEDFPVENWTAAESDISSLANTVDQAKQANGGRGSTTDGQANGKKVTMDDLWEDNWDDDVIESDFAHQLRAELEKTKSNGTGPTPMQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGJ8</accession>
    <name>E3KGJ8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08609</name>
        <name evidence="3" type="ORF">PGTG_19635</name>
    </gene>
    <organism evidence="2 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <reference evidence="2" key="3">
        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83423.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="DS178399">
        <property type="protein sequence ID" value="EFP93582.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327842.2">
        <property type="nucleotide sequence ID" value="XM_003327794.2"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338001.2">
        <property type="nucleotide sequence ID" value="XM_003337953.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KGJ8"/>
    <dbReference type="GeneID" id="10532100"/>
    <dbReference type="GeneID" id="10534789"/>
    <dbReference type="KEGG" id="pgr:PGTG_08609"/>
    <dbReference type="KEGG" id="pgr:PGTG_19635"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08609"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19635"/>
    <dbReference type="HOGENOM" id="CLU_572561_0_0_1"/>
    <dbReference type="InParanoid" id="E3KGJ8"/>
    <dbReference type="OrthoDB" id="2500287at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="74"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="198"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83423.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP93582.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="529" mass="57831" checksum="90A46E2BE4B29385" modified="2012-04-18" version="2">MASHHHQELDWDPAVADGWESLSGDFRPDLAPLPNHTDVQHHTYHNTTGPATRFIAPNPSHQYHYQTASGAPHLGAISTAGPGIPSESLAPSQRSTVAHSTGSLFHITNPAHSLTGPPAQVGSNGAQGAMQIADNSTPQSTSPLPMTSVGHRTQHTPTHGHPGQIGASEAGGIGPVRTPSQTSLPNLSPYPRNSTRDSVSNLSQHSSSSDSASTSIRSETLTYIRENYQQLQENLDNHEEFARIGQPLFSIPETERWPAAVALILLQGRANTASPATTMVLTNEDTPNLKTLVSSNIRLILLNPKLDIYGQRGSRTNLDAISPYSMMKTLLDSQSPRFLKQLPSNYNDNPAWLNSFDNLIKQQLKADKFKLSSLIQTNLPSPPEKVPSIKELVSSVFIAMHPRYKNTPKQQVYGQVPPPTRARLAYIRFMINLNRIQREGKKKGETPTIWHQINDDLNSRVGQTRMYRYAFGQLILAKDERLWDGQTALDEVDPDDCALPTEAEIATEIARLGGDTSTQFTQAAAGTAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGW9</accession>
    <name>E3KGW9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">chitin synthase</fullName>
            <ecNumber evidence="2">2.4.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_08730</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.4.1.16" evidence="2"/>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83544.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327963.2">
        <property type="nucleotide sequence ID" value="XM_003327915.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KGW9"/>
    <dbReference type="STRING" id="418459.E3KGW9"/>
    <dbReference type="GeneID" id="10532226"/>
    <dbReference type="KEGG" id="pgr:PGTG_08730"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08730"/>
    <dbReference type="eggNOG" id="KOG2571">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_002572_0_0_1"/>
    <dbReference type="InParanoid" id="E3KGW9"/>
    <dbReference type="OrthoDB" id="370884at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071944">
        <property type="term" value="C:cell periphery"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030428">
        <property type="term" value="C:cell septum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004100">
        <property type="term" value="F:chitin synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006031">
        <property type="term" value="P:chitin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd04190">
        <property type="entry name" value="Chitin_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004835">
        <property type="entry name" value="Chitin_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054295">
        <property type="entry name" value="CHS4-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914">
        <property type="entry name" value="CHITIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22914:SF41">
        <property type="entry name" value="CHITIN SYNTHASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03142">
        <property type="entry name" value="Chitin_synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22997">
        <property type="entry name" value="CHS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="4" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="87"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="313"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="819"/>
            <end position="838"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="850"/>
            <end position="869"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="876"/>
            <end position="900"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="1206"/>
            <end position="1226"/>
        </location>
    </feature>
    <feature type="domain" description="Chitin synthase 4-like" evidence="12">
        <location>
            <begin position="217"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1051"/>
            <end position="1170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="10">
        <location>
            <begin position="1084"/>
            <end position="1099"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1121"/>
            <end position="1157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF22997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP83544.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1236" mass="137700" checksum="92B62136A743CC66" modified="2012-04-18" version="2">MPEHSPDCKTLTRPERYVTPAPLINPTAPIRTTGFSNSFPPQQSNHPPWYSPWSLYVRLITLPIPSFFLSAIGIHQSNAQQAWREKVALCSIALLAALFTGFVTVGMNRALCPLTQKDSPGSMIPFDSHLPILGIRGWAFNVSKSNNPPGSPINFQSISQAHSGLDISTLFDQSHLPPSPSCQAPELLGPFAHSNLCYDPLSKSPAYSQLINASVPVGYTWDHVANLDGYMVINGIVLNLQPYLASNPHPIEQDPVDQAIRHMLQANLTSMGKDATRLFSYSNDLHRAIPCLISRFRAGSIDKITPGCFVSQLFLYVSLIVILGVVLVRFLMAIFFSWFLSDRLIRPPRNLRRKVISPAVLPEGANLSITNKNGAAPWANHLNNNNNSPQNSPTRPSNLKLFGAAISEKSSLLDPNSAQRNRKQQQQQQQKAALDSQGLINMAAIGAELFCVCLVTCYSEGAEGIKKTLDSISAADYSDARKLLFVVCDGMITGHGEKISTPDVCVGFLDADPLFGDPMPMSYRAVAAGAKEHNQAMVYAGHYTQVEGRRTPMIVIVKCGTAEEGKIEKKPGNRGKRDSQMILMNFFSRVTYNDRMSPLDYDLFRKIQVLMGVTPDFFEVCLMVDADTTVMKDSIGHLVNCMQHDPLIMGVCGETRITNKRQSWVTAIQVFEYYISHHLAKAFESVFGGVTCLPGCFSMYRLKARRMNDGDWVPILAQPEICREYSQSIVTTLHQKNLLLLGEDRFLTTLMIRTFPARKMMFCPQAKCRTLVPDEFAVLVSQRRRWINSTIHNLMELVRVPNLCGTFCFSMQFVVFMELMGTIVLPIAITLTYSLIINSILHPPKNFSDAIPLILLAAILGLPAILILATSRKVSYVFWMLIYLIALPVWNFVLPLYAFWHFDDFSWGETRKIQGEAKSQGHDEGSKTTFDANTIPHRRWEDWERSRLKKMKREERRRREFERTFGTQQYHLSTIGGSEYGGDTVSMVSSEDDRWGLAIGNYDSTEVARPPVGLYPVDDILSSVDGSTASTLNGDELGAILDQGWNDDLPFVEPKFPSPSKQTLVPPPSSSRLSPVAGGFISDHHHHHHHPYLHHHQQQHHQPNASATFNHPHFNHPPSNPYSSQQLPTSSSSSSSTFLIRDSPNPNSNPNPIIGSSRSTSPVNAFDPKPPQRYRLEELLTSLLPKPLSLSSLNFCSSSLLPPPPLSFFLGITCSLLLALFFVLLFPPLSLSLSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHB4</accession>
    <name>E3KHB4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_09402</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83689.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328108.2">
        <property type="nucleotide sequence ID" value="XM_003328060.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHB4"/>
    <dbReference type="GeneID" id="10532375"/>
    <dbReference type="KEGG" id="pgr:PGTG_09402"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09402"/>
    <dbReference type="HOGENOM" id="CLU_141178_0_0_1"/>
    <dbReference type="InParanoid" id="E3KHB4"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173584" evidence="2">
        <location>
            <begin position="22"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="106"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP83689.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="128" mass="14404" checksum="D65B05F23E2617BA" modified="2012-04-18" version="2">MNLIKLAIALIISTAFGLVAASNVIDFYTVEWRSFGCDPSEVALCIVTRYKKPNDRSSGPDFYILDEAVRVKPGSYNCGMTTAKCCGTNLDYRAIQTRNSHFMKDTTDRFCPNSPHYVDPPKKPRTRQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHL9</accession>
    <name>E3KHL9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">alpha-1,2-Mannosidase</fullName>
            <ecNumber evidence="12">3.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_09507</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) + 4 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 4 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56008"/>
            <dbReference type="Rhea" id="RHEA-COMP:14356"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:139493"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(4)-(alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) + 3 H2O = N(4)-(alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-beta-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2) + 3 beta-D-mannose</text>
            <dbReference type="Rhea" id="RHEA:56028"/>
            <dbReference type="Rhea" id="RHEA-COMP:14358"/>
            <dbReference type="Rhea" id="RHEA-COMP:14367"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28563"/>
            <dbReference type="ChEBI" id="CHEBI:59087"/>
            <dbReference type="ChEBI" id="CHEBI:60628"/>
            <dbReference type="EC" id="3.2.1.113"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.-" evidence="12"/>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83794.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328213.2">
        <property type="nucleotide sequence ID" value="XM_003328165.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHL9"/>
    <dbReference type="GeneID" id="10532481"/>
    <dbReference type="KEGG" id="pgr:PGTG_09507"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09507"/>
    <dbReference type="eggNOG" id="KOG2204">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003818_0_2_1"/>
    <dbReference type="InParanoid" id="E3KHL9"/>
    <dbReference type="OrthoDB" id="2504257at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050749">
        <property type="entry name" value="Glycosyl_Hydrolase_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742:SF101">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE 1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00747">
        <property type="entry name" value="GLYHDRLASE47"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="7" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="8 12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="disulfide bond" evidence="11">
        <location>
            <begin position="235"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP83794.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="372" mass="41982" checksum="EAD462DFF66F8B81" modified="2012-04-18" version="2">MGLKDEFDQGVSHTLKIDFSKSQTNDSVSLFETTIRYLGGMLSAYELSGSKNTALLKQARTLGDKLLTAWPDPRQNLPFPQLDFGRNRPVFKKKISSAEILIAEAGTLILELGRLSHHTQDPKYLRQAVKAMQAIMNSRSTFPGLAGFSLAVQSQAVKNDFATWGGGADSYYEYLLKYGILIDNQDESYLNAWKLAVKSSKQHLIQVSPVRNLTYLASFSSEKGGIRYEFSHLGCFAGGNWLLGGKVLDDPSTFQYGLRLVETCMETVEFFPANRVIGYRELEFFDKNGFYIGIESYDLRPEVVESAFYAWRLTGDTRYQDFVWEAFKSLQKHCKAPASFAAISDVNSLPVKLIDDSESFLYAELFKVERFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIH9</accession>
    <name>E3KIH9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Peptidyl-prolyl cis-trans isomerase</fullName>
            <shortName evidence="10">PPIase</shortName>
            <ecNumber evidence="10">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_10482</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 10">PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 10">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the cyclophilin-type PPIase family. PPIL4 subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="10"/>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84104.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328523.2">
        <property type="nucleotide sequence ID" value="XM_003328475.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIH9"/>
    <dbReference type="FunCoup" id="E3KIH9">
        <property type="interactions" value="902"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KIH9"/>
    <dbReference type="GeneID" id="10545202"/>
    <dbReference type="KEGG" id="pgr:PGTG_10482"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10482"/>
    <dbReference type="eggNOG" id="KOG0415">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018791_3_2_1"/>
    <dbReference type="InParanoid" id="E3KIH9"/>
    <dbReference type="OrthoDB" id="2083at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01921">
        <property type="entry name" value="cyclophilin_RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12235">
        <property type="entry name" value="RRM_PPIL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.100.10:FF:000015">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001243">
        <property type="entry name" value="Peptidyl-prolyl cis-trans isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.100.10">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035542">
        <property type="entry name" value="CRIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029000">
        <property type="entry name" value="Cyclophilin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002130">
        <property type="entry name" value="Cyclophilin-type_PPIase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035538">
        <property type="entry name" value="Cyclophilin_PPIL4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035979">
        <property type="entry name" value="RBD_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000504">
        <property type="entry name" value="RRM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45843">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45843:SF1">
        <property type="entry name" value="PEPTIDYL-PROLYL CIS-TRANS ISOMERASE-LIKE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00160">
        <property type="entry name" value="Pro_isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00076">
        <property type="entry name" value="RRM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00153">
        <property type="entry name" value="CSAPPISMRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00360">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50891">
        <property type="entry name" value="Cyclophilin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54928">
        <property type="entry name" value="RNA-binding domain, RBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50072">
        <property type="entry name" value="CSA_PPIASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50102">
        <property type="entry name" value="RRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6 10" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase cyclophilin-type" evidence="12">
        <location>
            <begin position="6"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="RRM" evidence="13">
        <location>
            <begin position="249"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="343"/>
            <end position="437"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="358"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="382"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="11">
        <location>
            <begin position="423"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU365081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EFP84104.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="437" mass="50547" checksum="B88250D08513B036" modified="2012-04-18" version="2">MSVLFETSLGDLVVDLEVELCPKTSENFLKLCKIYYYNFCSFFNVQKDFIAQTGDPTDLGTGGESIYARLERSDSHPDYGNRYFRSELATQKAHKSLNHGRRGTLAMAVSEGKCASQFFFTLADGLNELDGKHVVFGRLVEGFETLEQINNALVDDQGRPLRDIRLRHVIVLDDPFDDPPGILIPPQSPAEPSSHQGKVLRVGDDEELDQELDPEVVEERRREREARAQALTLEMVGDLPFAEVAPPENILFVCKLNSITRSEDLELIFSRFGTILSCEVIKDAKTGDSLQYAFIEFDQREDAERAYFKMDGVLIDDRRIHVDFSQSVSKLHSDWIFKRTGKRVKPNQTYEQRPKKRQPSERSPPRARRDSKYEMSFDDADLIERSARRSDHRRHDDRRPHSSRDHRSNHRQDSRAHHDSRHDHSRRRSRSPARRHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIT8</accession>
    <name>E3KIT8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glycosyltransferase family 49 protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_10591</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass type II membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84213.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328632.2">
        <property type="nucleotide sequence ID" value="XM_003328584.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIT8"/>
    <dbReference type="STRING" id="418459.E3KIT8"/>
    <dbReference type="GeneID" id="10545473"/>
    <dbReference type="KEGG" id="pgr:PGTG_10591"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10591"/>
    <dbReference type="HOGENOM" id="CLU_034771_1_1_1"/>
    <dbReference type="InParanoid" id="E3KIT8"/>
    <dbReference type="OrthoDB" id="411524at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015020">
        <property type="term" value="F:glucuronosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042285">
        <property type="term" value="F:xylosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035269">
        <property type="term" value="P:protein O-linked glycosylation via mannose"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051292">
        <property type="entry name" value="Xyl/GlcA_transferase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12270:SF25">
        <property type="entry name" value="GLYCOSYLTRANSFERASE-LIKE PROTEIN LARGE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12270">
        <property type="entry name" value="GLYCOSYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13896">
        <property type="entry name" value="Glyco_transf_49"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0735">Signal-anchor</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="43"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004606"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP84213.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="571" mass="64720" checksum="DE1EE3D5CC11CF25" modified="2012-04-18" version="2">MSTANLSSSHGSTSRWPRESSDQSGWNAFKRLTLLTRFLKPIWTVYLISAILYTTSVVVIFPGFHLLFDGLLTSRQSPVDPTLSAIQSSTIQIVPRLLPNHHGDSHSLDRLKIRKNLKGTSVNTDEVIAEDDASLWTARTGKVALRVTSPTSFRRTADGRPIPPRSTLKGFDPSFSDTVDWYPVGHNLSGQISSQDHLLQSARKEIHSTTEDFFLSKAFSDSLGPSKIIPFFYKALGPDQSGEINPKDISITTLVTSNRFKVFAKLVENYQGPISATIHVSDSPAILKPLLESLDELYHSSKSMSTWVDIHLVVDSFDRQFNMWRNVAKFFARTDYVMMLDVDFWICTNFRQRILGSPELLDKLAAGTSAFVIPAFEYTKQSDGVDPDGFPSDKQALMKLVSSGQIGMFHKSWKPGHGSTNYTRFYHADQLDQIYPASGYSHSYEPYVVYKKDKTPFCDERFIGYGANKAACIFELYLSGVSFYVLPQDFLIHQSHAYAEKTRHHERRFNRKLYSDFREEVCFRYFNEFMKLGLIYDKGQNMLGECQKIRGFAEAATRLGQANLVPINFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KK75</accession>
    <name>E3KK75_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SAM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10859</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329119.1">
        <property type="nucleotide sequence ID" value="XM_003329071.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KK75"/>
    <dbReference type="GeneID" id="10526667"/>
    <dbReference type="KEGG" id="pgr:PGTG_10859"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10859"/>
    <dbReference type="HOGENOM" id="CLU_1349484_0_0_1"/>
    <dbReference type="InParanoid" id="E3KK75"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="81"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="228" mass="25322" checksum="0A828E440C9A3196" modified="2011-01-11" version="1">MHTRVTTQPESEPQELMWPYLPAVRRPTPEYARPVPDSDPIATTRTYVMVEEHRRAAGPIGDSPNNNAANLWTRATGHATNANALDQNNPHKRSPSADLPHNRPNVRTRPNHPPPVLTAPEAPIDPARSQLTTTYDEDDPPAGPIARPDAPPLDWPSTPPDHIGELARFLIHCGIDLEDQHTREILLQHRINRWQHFILSNEAELTSLGITLGAARALCQEASNTNNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLB8</accession>
    <name>E3KLB8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11262</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP85093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329512.1">
        <property type="nucleotide sequence ID" value="XM_003329464.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLB8"/>
    <dbReference type="GeneID" id="10527074"/>
    <dbReference type="KEGG" id="pgr:PGTG_11262"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11262"/>
    <dbReference type="HOGENOM" id="CLU_918699_0_0_1"/>
    <dbReference type="InParanoid" id="E3KLB8"/>
    <dbReference type="OrthoDB" id="10414967at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="223"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="165"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="242"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="269"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="285" mass="31089" checksum="222C6BCFD6C960A6" modified="2011-01-11" version="1">MSSSSLSPLALKASRGPKKESTSLVSSCSTTTTKSTKTSSTSIKGKKQRKKQKQKSVSAGADDQVYWDPPQQSQIVRSIDQLRLHSPTHHGSTGALTKTRESTDTAAHPAHPPLRTPSRTDEPRTIASVIPETGKTNSTEPDPPAEGHHDVAKEDVRKRKRTNSTTTTTTTTTTTMDQARSSPSSSSSSRPPSRSDHQHTPPSKNTPILCQWNLRETGRLMSKFMAQKNAIPEPKYLVPVPKKPRSRSSHPKKYSKKASLANRFRHRLPAASSSKPSSSKPARSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLF2</accession>
    <name>E3KLF2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Histone H3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_11296</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="10">The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the histone H3 family.</text>
    </comment>
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        <property type="protein sequence ID" value="EFP85127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003329498.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLF2"/>
    <dbReference type="FunCoup" id="E3KLF2">
        <property type="interactions" value="500"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KLF2"/>
    <dbReference type="GeneID" id="10527108"/>
    <dbReference type="KEGG" id="pgr:PGTG_11296"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11296"/>
    <dbReference type="eggNOG" id="KOG1745">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_078295_4_0_1"/>
    <dbReference type="InParanoid" id="E3KLF2"/>
    <dbReference type="OMA" id="DSNRCAI"/>
    <dbReference type="OrthoDB" id="2501816at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000786">
        <property type="term" value="C:nucleosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030527">
        <property type="term" value="F:structural constituent of chromatin"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22911">
        <property type="entry name" value="HFD_H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.20.10:FF:000010">
        <property type="entry name" value="Histone H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007125">
        <property type="entry name" value="H2A/H2B/H3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000164">
        <property type="entry name" value="Histone_H3/CENP-A"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11426">
        <property type="entry name" value="HISTONE H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00125">
        <property type="entry name" value="Histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00622">
        <property type="entry name" value="HISTONEH3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00428">
        <property type="entry name" value="H3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00322">
        <property type="entry name" value="HISTONE_H3_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00959">
        <property type="entry name" value="HISTONE_H3_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0007">Acetylation</keyword>
    <keyword evidence="4" id="KW-0158">Chromosome</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="9 10" id="KW-0544">Nucleosome core</keyword>
    <keyword evidence="8 10" id="KW-0539">Nucleus</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Core Histone H2A/H2B/H3" evidence="12">
        <location>
            <begin position="44"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="11">
        <location>
            <begin position="19"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP85127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="136" mass="15281" checksum="A32190F3551DC666" modified="2011-01-11" version="1">MARTKQTARKSTGGKAPRKQLAAKAARKSAPATGGVKKPHRYKPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAIGALQESAEAYLVSLFEDTNLAAIHAKRVTIQPKDIQLARRLRGERS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KM09</accession>
    <name>E3KM09_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11527</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178294">
        <property type="protein sequence ID" value="EFP85358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329777.1">
        <property type="nucleotide sequence ID" value="XM_003329729.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KM09"/>
    <dbReference type="STRING" id="418459.E3KM09"/>
    <dbReference type="GeneID" id="10543901"/>
    <dbReference type="KEGG" id="pgr:PGTG_11527"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11527"/>
    <dbReference type="HOGENOM" id="CLU_2224510_0_0_1"/>
    <dbReference type="InParanoid" id="E3KM09"/>
    <dbReference type="OrthoDB" id="10441619at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="46"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="106" mass="12105" checksum="A9C9F077C585E564" modified="2011-01-11" version="1">MVEVRYSSWRIEPKCLARTDRQRAKIRGAGEAARSKKQKSKTSAGIEEKFLRGERSDSEDPETSEQPRKSGGLNEFMKNVKQRASQGCNISRELEWECKPNMGVSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNS0</accession>
    <name>E3KNS0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11701</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178297">
        <property type="protein sequence ID" value="EFP85945.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330364.2">
        <property type="nucleotide sequence ID" value="XM_003330316.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNS0"/>
    <dbReference type="GeneID" id="10539944"/>
    <dbReference type="KEGG" id="pgr:PGTG_11701"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11701"/>
    <dbReference type="HOGENOM" id="CLU_1778346_0_0_1"/>
    <dbReference type="InParanoid" id="E3KNS0"/>
    <dbReference type="OrthoDB" id="2495430at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174047" evidence="1">
        <location>
            <begin position="22"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85945.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="147" mass="16930" checksum="2B121F08DEF00D2C" modified="2012-04-18" version="2">MNRYAFFIALWFASLITNSVSVNTGHKGEYIPCRRWEPISALFGQKLEEMRPLLRDTVWEKITEKGTQTEPFKHAVGDRAASFTWCTERGRGAVVKNTGSNDLVYALRHHTSQKFVIGARLQAGDYQHVEFPEECTKDEVYLHIRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>E3KP90</accession>
    <name>E3KP90_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Glutamine synthetase</fullName>
            <ecNumber evidence="3 11">6.3.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_12071</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 11">
            <text>L-glutamate + NH4(+) + ATP = L-glutamine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16169"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9 10">Belongs to the glutamine synthetase family.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="3 11"/>
    <dbReference type="EMBL" id="DS178298">
        <property type="protein sequence ID" value="EFP86115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330534.1">
        <property type="nucleotide sequence ID" value="XM_003330486.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KP90"/>
    <dbReference type="FunCoup" id="E3KP90">
        <property type="interactions" value="485"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KP90"/>
    <dbReference type="GeneID" id="10540123"/>
    <dbReference type="KEGG" id="pgr:PGTG_12071"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12071"/>
    <dbReference type="eggNOG" id="KOG0683">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036762_1_1_1"/>
    <dbReference type="InParanoid" id="E3KP90"/>
    <dbReference type="OMA" id="DRRPNAN"/>
    <dbReference type="OrthoDB" id="1936100at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.70:FF:000004">
        <property type="entry name" value="Glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000004">
        <property type="entry name" value="Glutamine synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027302">
        <property type="entry name" value="Gln_synth_N_conserv_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050292">
        <property type="entry name" value="Glutamine_Synthetase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852">
        <property type="entry name" value="GLUTAMINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20852:SF57">
        <property type="entry name" value="GLUTAMINE SYNTHETASE 2 CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03951">
        <property type="entry name" value="Gln-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00180">
        <property type="entry name" value="GLNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00181">
        <property type="entry name" value="GLNA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51986">
        <property type="entry name" value="GS_BETA_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS beta-grasp" evidence="12">
        <location>
            <begin position="26"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="GS catalytic" evidence="13">
        <location>
            <begin position="115"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU004356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EFP86115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="361" mass="39453" checksum="CA47BFF78A071733" modified="2011-01-11" version="1">MSVCPTTSAPGHLAKYMDLPQPENLIQAEYVWIDGSGGLRCKTTTMDLPASGQVTVADCKEWNFDGSSTAQASGHDSDVFLRPAAVFKDPFRRGKNVLVLAECYNSDGTPNKTNFRYSCKKSMDAAAQHKPWFGIEQEYTLFDASGTSPYGWPAGGFPGPQGPYYCGVGTGKVFARDIVEAHYRCCLYAGVTISGVNAEVMPSQWEFQVGPCEGIDMGDHLTMARFLLYRVTEDWGIKVSFHPKPLAGDWNGAGAHTNYSTAATRQPGGIKAIHEYIEKLGKRHNEHIAVYGADNDQRLTGRHETGHIGNFSSGVAHRGASIRIPRHVEATGEGYLEDRRPASNIDPYQVTGIIVETTLLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="81" xmlns="https://uniprot.org/uniprot">
    <accession>E3KP97</accession>
    <name>E3KP97_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PGTG_12078</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the PI3/PI4-kinase family. ATM subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="DS178298">
        <property type="protein sequence ID" value="EFP86122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330541.1">
        <property type="nucleotide sequence ID" value="XM_003330493.2"/>
    </dbReference>
    <dbReference type="FunCoup" id="E3KP97">
        <property type="interactions" value="571"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KP97"/>
    <dbReference type="GeneID" id="10540130"/>
    <dbReference type="KEGG" id="pgr:PGTG_12078"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12078"/>
    <dbReference type="eggNOG" id="KOG0890">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000178_0_0_1"/>
    <dbReference type="InParanoid" id="E3KP97"/>
    <dbReference type="OMA" id="YTVYSQM"/>
    <dbReference type="OrthoDB" id="381190at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000077">
        <property type="term" value="P:DNA damage checkpoint signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000723">
        <property type="term" value="P:telomere maintenance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00892">
        <property type="entry name" value="PIKKc_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1070.11:FF:000032">
        <property type="entry name" value="Protein kinase rad3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1070.11">
        <property type="entry name" value="Phosphatidylinositol 3-/4-kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1010.10">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056802">
        <property type="entry name" value="ATR-like_M-HEAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050517">
        <property type="entry name" value="DDR_Repair_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003152">
        <property type="entry name" value="FATC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057564">
        <property type="entry name" value="HEAT_ATR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000403">
        <property type="entry name" value="PI3/4_kinase_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036940">
        <property type="entry name" value="PI3/4_kinase_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018936">
        <property type="entry name" value="PI3/4_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003151">
        <property type="entry name" value="PIK-rel_kinase_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014009">
        <property type="entry name" value="PIK_FAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012993">
        <property type="entry name" value="UME"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139">
        <property type="entry name" value="ATAXIA TELANGIECTASIA MUTATED ATM -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11139:SF125">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE MEC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02259">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02260">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23593">
        <property type="entry name" value="HEAT_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25030">
        <property type="entry name" value="M-HEAT_ATR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00454">
        <property type="entry name" value="PI3_PI4_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08064">
        <property type="entry name" value="UME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01343">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00146">
        <property type="entry name" value="PI3Kc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00802">
        <property type="entry name" value="UME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51189">
        <property type="entry name" value="FAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51190">
        <property type="entry name" value="FATC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00916">
        <property type="entry name" value="PI3_4_KINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50290">
        <property type="entry name" value="PI3_4_KINASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0227">DNA damage</keyword>
    <keyword evidence="10" id="KW-0234">DNA repair</keyword>
    <keyword evidence="8" id="KW-0418">Kinase</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-0539">Nucleus</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="FAT" evidence="16">
        <location>
            <begin position="1660"/>
            <end position="2265"/>
        </location>
    </feature>
    <feature type="domain" description="PI3K/PI4K catalytic" evidence="15">
        <location>
            <begin position="2378"/>
            <end position="2698"/>
        </location>
    </feature>
    <feature type="domain" description="FATC" evidence="17">
        <location>
            <begin position="2698"/>
            <end position="2730"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="555"/>
            <end position="606"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="14">
        <location>
            <begin position="584"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EFP86122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="2730" mass="308052" checksum="7AACB57D316EE257" modified="2011-01-11" version="1">MSFQTLNNAFGTNEAPASTARILESIIRPSSTLEGGHSTSSLGIEQATALIRVVFNTSLDQFFGIHRWQTKMALHKQKDLSDATKNVLQFTFGQEFCRTPVLLIRRFLLAKDHQDLIERAQGATSRTQAEGENTSPAVLEEELSIWLLIRMLYCAALIRDSPMEYLPEDFRADRIDFVHRLEASSVSIHYYLFCSTDREIRAVGTARAVDFAKFWTTINEEIRSRLMRWMEGSDHLGYIELLGTDRRTQIPLTQDPVDIFQLEQRPFRIPIISQPFAIHLLLSSLRIHFRIIHHSIYFLETIPSAVEALITLWEYTMKSFPDDHDQRVLEDLTDMLGWFFEEPTEPTQLVLVTTPKEVPNHLANYIKSRSPTSLDKICSAIVDQCSKLFAPSTSSDQSQLAGKPCSQNIIVLFLKILRWLADENGKMSMSQTSGSITRLVVKHGKSMIEDLLSNSEAALNIANPNLQMMSLALLCCPMDTRYGRAKYRHTSQGTYVNYELMDYTSIYRFESLLSMFTAKWIQQISAQKYCTELGRFLHWLVQSLQAIHLRLNTTRTSSRRKRKRGDPEELDHAGPFSFNDDNETSATTNVLPSRSQHQLPNNSTSSPTTLHCNLISLLTSCSALAELPADLGKGSLTPIILKSLERITSLISHRPEDYEAQIKMFQTLERGFDLLGALICNSALEQPGFLNATNSDDLAYLPTDDLRNLFQCPSCDRSHLDPTKPKSSESQFLPRCIFTSAIMLCYNSDLLSYLSQKIDKLTKKEKSIQLAKSGARLAFIRFLTRVLNHTVIGDLSSIKESPIVCTSEVNMRIASVLERGNRMERIAAGRMLAGVFSNSLHLLSIWPAGQLSDFQLDPVLSQLGSFAKASSLKVQETLAISIVHCFELTRATDLELVDQGRVELDKSLGHRICTILLRQMCRRCAYLLGVIKTEMSFFSRRIGLSIYASLDRYLPSLSQFMLIDLKFDSYGSTLFADLLGISLEIFLTSTARSTLPLLVLNSNTTTIEAIAKAKEETVPGLLVYQAADILTELCSRNTTKELKKGAEIFYRLLNSGRENSSDPNQISIQIGKLMEMSAGLIYYRLIVKVGSEADRSRGPERVKAALRKALILKKSLADSSEISSDKEIQEELRENILPILSYMNGSLQDLRGKITMAEKIKVMHGLGNLIYLVDSGVSSYTPQIMTSLQASLAIPVLRSATLQTWDIFVKVTPLNDLMPFIGQITAAVVGVWPQMTADQIEVSISMLQHILRQRDRLGHYAFDIADLSGLSTSAIPSHDFPRVHPALVGIVQEQAGLKNNLTWTEKLKNLIGRINSESEIVIRQSLKELTTLLENDPEKMKMLMAGDTFHHLVGDVVKALIGVTSRCSDASDDIKSMAFECLGTVGAVDPDRCEISDEKSEMFLVSNFSDHDESVNFALHLLQTELIGGYRSTHDSRFHTFLTYAIQELLRFCGFTHDLVDPKKSANVPSSIKKKWHSMPKHILDSISPLLSSKFRFSFTNNISNNVPTYSHTTLYNSWLQNWLLRLITLVKNKDATDIFLPFLGTIRNGDSVISQKLLPHIALHIVISGSQEELENMKLEIVTVLEDQVERRSGFSPEGRQLVAQTIFGLMDHFSRWIRDRQKAQSAVNEHGGAKRRDHKTDADTMRVKSIMSQISPELIASAALYCKDHARALLNIEQQIVKLEGGQVASGQSMIVDDAPDEISPEEQLQKYYEKAHEIYAAVDEPDGMEGISAKITAPSIPHQIREHESTGRWTSAQSCWEVQLQRHPDELRSHLGLLRCLRNLGHYDSLRAHIVGVLQSHPDWERDLAPFSVESSLVSNNWEGLTRAVQVGSLESPEVIFGKVVDMLLNSDEQSFNEAMKDARIRLGNQILGASRKDAYKRMYDSAIYLHVLHELPLIDQACRDSSPAAGLSKIGLKAGLTTDRNLMNHLDFRLESISPAFRYREQLLRLRRATFQLRSRGAPAVGQLWVQTAKIARKAGHLQTAYSAVLQASELKAPTAFIQQAKLMKMEDQLYKAVLKLDDNLKNSPGKLIDGSFDRLNQLCPRDYAKASLQRVRWMDEVDRYSANHIIEHFNKVCAENPNWASSHYYFGRFYDDKASQVAPSIRTSTNQSRSTVAEWTYHCCKHFQKSLMFGTKFIYQALPRLLTLYFSFGEHPNLLAIFTKVESKGRKDGVEIHQEDYTAALRGDDLGGIFLRVDKVIQNAVKRLPVFEWLTVLPQVVSRVMHKSTHVQAIVHKILTHVLRSYPDQALWAMVSGVESSNSARSSRCTWVLQDATDVANSANPQLEPQNLASKIAQCRKIVKQLLRLCNFPIKNNAKHLSLHDVFPALQQCTPCDLLIPVQHSLIASLPPNDVNFANHQPFPSDLPCISSFIDKITIMSSMQKPRKIGIIGSDGKVYPFLCKPKDDLRKDARLMEFNSMINKLLKKDSESRRRNLHIRTYAVVVLNEECGLLEWVSNTIPFRHLLTGLYAPKGIQLWSNELKLLSEKIRKYRDEWDKVKDIFEKEILPKFPSVFHQWFLNNFPDPTSWLKSRQAYGRTCAVMSMVGFVLGLGDRHGENILFDSTNGDVVHVDFNCLFDKGRTFEVSENVPFRLTHNLVSGLGITGVEGVFRRASEVTMGILRNNKDSLMSVLETFVHDPLVDWMPSVSKRKGADAPTEEYVAREAKKALEPISRKLTGFQITSSVSGKCDRQMSTENQVDSLINEARDNRHLGRMYFGWGPYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPT0</accession>
    <name>E3KPT0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12271</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178299">
        <property type="protein sequence ID" value="EFP86315.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330734.2">
        <property type="nucleotide sequence ID" value="XM_003330686.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KPT0"/>
    <dbReference type="FunCoup" id="E3KPT0">
        <property type="interactions" value="66"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KPT0"/>
    <dbReference type="GeneID" id="10540328"/>
    <dbReference type="KEGG" id="pgr:PGTG_12271"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12271"/>
    <dbReference type="eggNOG" id="ENOG502QRTT">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027878_0_0_1"/>
    <dbReference type="InParanoid" id="E3KPT0"/>
    <dbReference type="OrthoDB" id="5577218at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032541">
        <property type="term" value="C:cortical endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097038">
        <property type="term" value="C:perinuclear endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048309">
        <property type="term" value="P:endoplasmic reticulum inheritance"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000921">
        <property type="term" value="P:septin ring assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013635">
        <property type="entry name" value="Ice2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31726">
        <property type="entry name" value="PROTEIN ICE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31726:SF2">
        <property type="entry name" value="PROTEIN ICE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08426">
        <property type="entry name" value="ICE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="114"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="154"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="184"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="257"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="327"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="358"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86315.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="397" mass="43964" checksum="78DAFFCFBC08621F" modified="2012-04-18" version="2">MINRLITRAGRTASFLQILLYLPLALDIAGKDCFLALSASLALYYSALSTLYLIFRNTRFAFVSKVFGLLQNLVIPTFLLFWLNIYSPDESKNIGNPILASNLIGRMLSLWEVVLTWSTPVFVLLEGISTLLCIQSVGQISRYLVEAKNESYSFVFLVLSAAVYVGSAYFLFDSYAYAARESLSATLIGVSVTSVAFLSGIAISNRKGNVVETSLMMAYLSYNIYWLSSEKLDPVSFFSSFKSEAVPPLPPMILRSATAIIGFVSTTFGAGLDFLLACSSALPLPVFINLLYRVVALYGASRIVVAIKRANSGFAYTRRLSDEEPMVRIMTVIVSYSRFVLISVYTHLLLLNNEGHQVFWRWINVFVTLGLWGVELMIGKEEGDNDSIVTGLGLKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQM8</accession>
    <name>E3KQM8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="1">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_12985</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="8">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="1"/>
    <dbReference type="EMBL" id="DS178301">
        <property type="protein sequence ID" value="EFP86603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331022.1">
        <property type="nucleotide sequence ID" value="XM_003330974.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQM8"/>
    <dbReference type="STRING" id="418459.E3KQM8"/>
    <dbReference type="GeneID" id="10540703"/>
    <dbReference type="KEGG" id="pgr:PGTG_12985"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12985"/>
    <dbReference type="HOGENOM" id="CLU_019279_0_2_1"/>
    <dbReference type="InParanoid" id="E3KQM8"/>
    <dbReference type="OrthoDB" id="5800476at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006282">
        <property type="term" value="P:regulation of DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000538">
        <property type="entry name" value="Putative Casein kinase I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050235">
        <property type="entry name" value="CK1_Ser-Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11909">
        <property type="entry name" value="CASEIN KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="9"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="284"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP86603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="345" mass="38860" checksum="E69C7405C14BA903" modified="2011-01-11" version="1">MDLRVGGKYRLGKKIGCGSFGDIYLGINIISGEEVAIKLESVKAKHPQLEYEAKVYKTLAGGVGIPFVRWFGVECDYNAMVIDLLGPSLEDLFNFCQRKFSLKTTLLLADQLISRIEYVHSRNFIHRDIKPDNFLMGIAKRGNQVNIIDFGLAKKYRDPKTLLHIPYRENKNLTGTARYTSINTHLGVEQSRRDDMESLGYRRLEAKVRRSWEEDDPSTEFSAGSEELRSTQLLALFEVDDSLTTAIFASSSVTCLFARVTRTTTSLIGRSNPQRRRLFSAHQRQMAAKDRSSAAAPAPSPAAPLRRKVIPEAAPLPASGHDRKLRSQTKTVEEQRQAFYDEFAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KR99</accession>
    <name>E3KR99_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_13206</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178302">
        <property type="protein sequence ID" value="EFP86824.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331243.2">
        <property type="nucleotide sequence ID" value="XM_003331195.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KR99"/>
    <dbReference type="GeneID" id="10541411"/>
    <dbReference type="KEGG" id="pgr:PGTG_13206"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13206"/>
    <dbReference type="HOGENOM" id="CLU_642717_0_0_1"/>
    <dbReference type="InParanoid" id="E3KR99"/>
    <dbReference type="OrthoDB" id="2502591at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174080" evidence="2">
        <location>
            <begin position="24"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="59"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP86824.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="427" mass="49052" checksum="710E783E9D2EF714" modified="2012-04-18" version="2">MRLKISFVFIALCTLKASQLALSMEEVVGLGKDVVPQMIRHDSPLGAKMNAPMSGCSGLDQSSQTALTSSHPELASANQGKSPCSQIKELRNSIEIHGHRHLEDVTQNLEQLHNYLTPVGETKPPPAFQEPQNLVEPLFEYKYKTLGWNQNFLKVLPGSRGTRQLASTTTPLEGITQVVLIWKYLQETFINLKCQDLVDPEIKLKFLKSLFLLGDYIHENSLLPQAEIKQIKIFQPETVVNLFNFHFNLLIRSKGNEFFGSPEWVNPELELLLSNPYLKHFHRSILDESQYFLKKAEEIKYPEWFDGVQIRDLLFHDPIVNHEHPKLKLDSYQFLTLYTISKFVDSHLPHLVPELDKSQEAVLLDKQGQYISSLLKLLPSRFKDPRLQAVLKQKEDLIKIHASQLHKQKKTKDWIKTVKKLIHKASN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>E3KRS4</accession>
    <name>E3KRS4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ras-protein rsr1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_12740</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178303">
        <property type="protein sequence ID" value="EFP86999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331418.1">
        <property type="nucleotide sequence ID" value="XM_003331370.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KRS4"/>
    <dbReference type="STRING" id="418459.E3KRS4"/>
    <dbReference type="GeneID" id="10541913"/>
    <dbReference type="KEGG" id="pgr:PGTG_12740"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12740"/>
    <dbReference type="eggNOG" id="KOG0395">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_041217_9_8_1"/>
    <dbReference type="InParanoid" id="E3KRS4"/>
    <dbReference type="OMA" id="MPLREFK"/>
    <dbReference type="OrthoDB" id="5976022at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019003">
        <property type="term" value="F:GDP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002075">
        <property type="entry name" value="Probable RSR1-GTP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001806">
        <property type="entry name" value="Small_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020849">
        <property type="entry name" value="Small_GTPase_Ras-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24070">
        <property type="entry name" value="RAS, DI-RAS, AND RHEB FAMILY MEMBERS OF SMALL GTPASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00071">
        <property type="entry name" value="Ras"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SMART" id="SM00175">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00176">
        <property type="entry name" value="RAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00173">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00174">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51419">
        <property type="entry name" value="RAB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51421">
        <property type="entry name" value="RAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51420">
        <property type="entry name" value="RHO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="179"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="4">
        <location>
            <begin position="187"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP86999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="205" mass="22747" checksum="B5A32D218A268932" modified="2011-01-11" version="1">MKEYKIVIMGGGGVGKSALTVQFVHDMFVERYDPTIEDSYRKIITVDGNKVTVEILDTAGTEQFVALHSLYVKSGDGFLLVFALNTLDSIHELEALHESIVRIKEAEGYTDKIPIVLCGNKCDLEERIVQRSWAVALSQEWGGVPYYETSARKRINVDEVIADLVRQIIKADRLGHLNHGGPGRHGLGGKKRRKRVTPNGGCSIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KRV1</accession>
    <name>E3KRV1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_12767</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178303">
        <property type="protein sequence ID" value="EFP87026.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331445.2">
        <property type="nucleotide sequence ID" value="XM_003331397.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KRV1"/>
    <dbReference type="GeneID" id="10542103"/>
    <dbReference type="KEGG" id="pgr:PGTG_12767"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12767"/>
    <dbReference type="HOGENOM" id="CLU_2293064_0_0_1"/>
    <dbReference type="InParanoid" id="E3KRV1"/>
    <dbReference type="OrthoDB" id="1861185at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP87026.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="101" mass="11022" checksum="20FCED9F4584BFFF" modified="2012-04-18" version="2">MAHKMGMVDNLVMYTAKNLGMDMVDHLGMDMADHLGMDMADHLGMDMADHLGMDMADHLGMDMADHLGMDMADHLGMDMADQPGMGTVDHLGIAEPHPQEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KS30</accession>
    <name>E3KS30_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13324</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EFP87105.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331524.2">
        <property type="nucleotide sequence ID" value="XM_003331476.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KS30"/>
    <dbReference type="STRING" id="418459.E3KS30"/>
    <dbReference type="GeneID" id="10542347"/>
    <dbReference type="KEGG" id="pgr:PGTG_13324"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13324"/>
    <dbReference type="HOGENOM" id="CLU_1759723_0_0_1"/>
    <dbReference type="InParanoid" id="E3KS30"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="58"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="81"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="104"/>
            <end position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87105.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="148" mass="15650" checksum="F4D0C4D73EA6F2D2" modified="2012-04-18" version="2">MMMGQLGVGGGAPPEREGSSERAIKSRPTSTLGRVLIPADSGYTRRNHHPFVSSLDPAIASFTSSSSSSTISKKNNPARVDGSSLLSSSTTVSDTLHSSDKSPTPTPPPPPPSLTNPTRDNHSLAALPFTRAPNSRLRIVNKRIKYST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3KST5</accession>
    <name>E3KST5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Cutinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_13693</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP87465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003331836.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KST5"/>
    <dbReference type="STRING" id="418459.E3KST5"/>
    <dbReference type="ESTHER" id="pucgt-e3kst5">
        <property type="family name" value="Cutinase"/>
    </dbReference>
    <dbReference type="GeneID" id="10538195"/>
    <dbReference type="KEGG" id="pgr:PGTG_13693"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13693"/>
    <dbReference type="eggNOG" id="ENOG502S6TR">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_067629_0_0_1"/>
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    <dbReference type="OMA" id="KEFCAQG"/>
    <dbReference type="OrthoDB" id="2586582at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="F:carboxylic ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016042">
        <property type="term" value="P:lipid catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000675">
        <property type="entry name" value="Cutinase/axe"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33630:SF9">
        <property type="entry name" value="CUTINASE 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33630">
        <property type="entry name" value="CUTINASE RV1984C-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01083">
        <property type="entry name" value="Cutinase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Cutinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
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    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="4">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173862" description="Cutinase" evidence="4">
        <location>
            <begin position="23"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="48"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="124"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="133"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP87465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="7">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="358" mass="34887" checksum="B9D2455718B7A009" modified="2011-01-11" version="1">MVSISFKSFLLLVINLQESVKSASVNRATLSKRQFDLSSLTGSLGGGSIPGIGGTSGSGSGSGGSGLGGFPSIGSGTGGGLGGLGSGSAGGLGSFPGMGSGGGTGGLGGLSGMASGSGIGGLGGLPGMGSGSSGTASSSNSDGASLNGQSKSQGSSGGCPSYYMIGARGTSEGPGGSIAYNALYQKVLASVPGGAKEELQYSTSADYSVTVTQGAQTEVKMITGEISKCPKTVFVLLGYSKGAMVQTQTLNNKEIPQDKIAAVALFGNPYFRAGAPQNKCGASSGMGIAAMAGAKMPDQLTDRVYDCCVPGDEICQTAGSIMGHLSYAGQHENDATEFVIQKLKAKLSGATGAQKGSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KUD4</accession>
    <name>E3KUD4_PUCGT</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_14624</name>
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    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178309">
        <property type="protein sequence ID" value="EFP87909.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003332280.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KUD4"/>
    <dbReference type="GeneID" id="10534888"/>
    <dbReference type="KEGG" id="pgr:PGTG_14624"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14624"/>
    <dbReference type="HOGENOM" id="CLU_163527_0_0_1"/>
    <dbReference type="InParanoid" id="E3KUD4"/>
    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP87909.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="93" mass="10683" checksum="EA82ABA4328A1E54" modified="2012-04-18" version="2">MTYAQLVMIHYYKNKQDSKSQWTAIDDRLRILQASSKEFQQVCSHAQLVMDKDDQLFPNKRHITQINKDEFTVPTLDDVQATMAAGNVANHSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KUH0</accession>
    <name>E3KUH0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13724</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178310">
        <property type="protein sequence ID" value="EFP87920.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332339.2">
        <property type="nucleotide sequence ID" value="XM_003332291.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KUH0"/>
    <dbReference type="GeneID" id="10534899"/>
    <dbReference type="KEGG" id="pgr:PGTG_13724"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13724"/>
    <dbReference type="HOGENOM" id="CLU_2307430_0_0_1"/>
    <dbReference type="InParanoid" id="E3KUH0"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="11"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87920.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="100" mass="10909" checksum="40AF6FF7033DC74E" modified="2012-04-18" version="2">MAETRATQAHQTRSSSKRTSSAAKTQATHKKGHAVRKQASSSRKKATSGKRQPNHTDENTDENTDEDDHHDTTPEPTETQQHRSSATESGQCTPAPTISN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KUR8</accession>
    <name>E3KUR8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_13822</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178310">
        <property type="protein sequence ID" value="EFP88018.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003332389.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KUR8"/>
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    <dbReference type="KEGG" id="pgr:PGTG_13822"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13822"/>
    <dbReference type="HOGENOM" id="CLU_053900_1_0_1"/>
    <dbReference type="InParanoid" id="E3KUR8"/>
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    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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            <end position="71"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="174"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="40"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="105"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP88018.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="414" mass="46291" checksum="BAEFD20718AFB97D" modified="2012-04-18" version="2">MVVLRKQLAQLQSKLKALESRLEKEVSARIATIKDNSIINDVHSTSPPYPSVKSSSIQKSTRSAQSRDQRTLRSRSFKLMRHLINIVDVSRAASTASELEKKRTTIGSSRDPVTSVSSVTELKSSAKPTEATNSPPTKLKASAKTTLGLSLKEKAVRSSNQNDFQASTILLPPITEPEAPVNPKSAKTPPIPPNNLVTALNTSSETPRLFHPKRTRKADLASSNKEANERNLQAVSELPVTKELNTNAHSELEGAVPKTSETLAITRTSGNKKAEIIEHNDLRRGCSRFALLKYDPAVLDLLPTSVKEKRFIRTSTVTDIENLQQASEILVRRQVLGIWEWREGTSKATLHEYKTPELGTTMSNRRFKFVGFAWKGALGQQPPRIEELHEDISQGADTLRWYWFKDLWLLFYFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVH0</accession>
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        <recommendedName>
            <fullName evidence="3">Chaperone DnaJ C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
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        <name evidence="4" type="ORF">PGTG_14394</name>
    </gene>
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP88310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003332681.1"/>
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    <dbReference type="STRING" id="418459.E3KVH0"/>
    <dbReference type="GeneID" id="10535702"/>
    <dbReference type="KEGG" id="pgr:PGTG_14394"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14394"/>
    <dbReference type="eggNOG" id="KOG0714">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_065208_0_0_1"/>
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    <dbReference type="OMA" id="RSHEGMH"/>
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    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
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    <dbReference type="GO" id="GO:0051087">
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        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006413">
        <property type="term" value="P:translational initiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd10747">
        <property type="entry name" value="DnaJ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.260.20:FF:000049">
        <property type="entry name" value="Unplaced genomic scaffold supercont1.14, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="Urease metallochaperone UreE, N-terminal domain"/>
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    <dbReference type="PANTHER" id="PTHR24078:SF553">
        <property type="entry name" value="DNAJ HOMOLOG SUBFAMILY B MEMBER 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24078">
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        <property type="match status" value="1"/>
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    <dbReference type="SUPFAM" id="SSF49493">
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            <end position="263"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="28"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP88310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="294" mass="32217" checksum="A42B19D63FEEC800" modified="2011-01-11" version="1">MPGAGGGGGGKKDMDINELFDSVLGRKSALFPDHGGRQSGGNPRGSSKHGPTPRDEHMNPFDNGTAPLFGDMRRETNRFGDTLFGNGVGAPQDTFMTLELSLKDLYLGVVKGFKFTRRLWDGKVEKIVRKVDIKPGWKDGTKIRFEGLGDEDEDGRAGALVFVVAQKPDPKFKREGDDLIYTHTITLAEALAGPEPEHDLNRGLKHLDGRNVVFKLPHIGATGGRPVWPGFEIRIPGQGMPITRKSAGRLRGDLRVQIHITFPAWLSAAQIQAARQLKECRSLCCSFTLPSCNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVP1</accession>
    <name>E3KVP1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
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    <gene>
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    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycetes</taxon>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178313">
        <property type="protein sequence ID" value="EFP88381.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332800.2">
        <property type="nucleotide sequence ID" value="XM_003332752.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KVP1"/>
    <dbReference type="GeneID" id="10535854"/>
    <dbReference type="KEGG" id="pgr:PGTG_14465"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14465"/>
    <dbReference type="HOGENOM" id="CLU_1435078_0_0_1"/>
    <dbReference type="InParanoid" id="E3KVP1"/>
    <dbReference type="OrthoDB" id="10353050at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172240" evidence="2">
        <location>
            <begin position="28"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="152"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP88381.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="189" mass="20044" checksum="E4AAEAF5472782D7" modified="2012-04-18" version="2">MSTPQRSLKRFSLHLSVVCLFILAVPAGCPVAKPGVTDRMTTVTYMPDASSKELIATGGTGALVQLPKGSLIRFWNESGSSSSHTKVKTNLDKILPGGDRVEPGDEKIGEIPESSILKTQITSGNPNALENQNGKTRNGLSKIPVQSVSKPPSRPAPGVPRLPPPTRAPPELPASHVLQVTRQRSSEGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVT6</accession>
    <name>E3KVT6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF7143 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14386</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178313">
        <property type="protein sequence ID" value="EFP88302.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332721.2">
        <property type="nucleotide sequence ID" value="XM_003332673.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KVT6"/>
    <dbReference type="GeneID" id="10535690"/>
    <dbReference type="KEGG" id="pgr:PGTG_14386"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14386"/>
    <dbReference type="HOGENOM" id="CLU_1455095_0_0_1"/>
    <dbReference type="InParanoid" id="E3KVT6"/>
    <dbReference type="OrthoDB" id="2507284at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055567">
        <property type="entry name" value="DUF7143"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37592">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37592:SF1">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23631">
        <property type="entry name" value="DUF7143"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF7143" evidence="1">
        <location>
            <begin position="35"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF23631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88302.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="186" mass="20394" checksum="A88A38FA8A1DFAED" modified="2012-04-18" version="2">MILTADPNIRFENLGLAALNRKSAYLVQCELPCYMTGKTPLPKDVIPPKDVTCLDTKIFLDIPDVTIDGKKYSEIDFKTQGKDLTPAGYALATFTAGGDNTAESLGTANKLYTAVNAALRDRGNRSILHQLKVVDFFIGSQIAATQGPEGKKKQIRNLNKTIKNCGHCTAEERQKFQDMLTKAEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVT8</accession>
    <name>E3KVT8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RxLR effector candidate protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_14388</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178313">
        <property type="protein sequence ID" value="EFP88304.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332723.2">
        <property type="nucleotide sequence ID" value="XM_003332675.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KVT8"/>
    <dbReference type="GeneID" id="10535696"/>
    <dbReference type="KEGG" id="pgr:PGTG_14388"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14388"/>
    <dbReference type="HOGENOM" id="CLU_067388_0_0_1"/>
    <dbReference type="InParanoid" id="E3KVT8"/>
    <dbReference type="OrthoDB" id="2499147at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172874" description="RxLR effector candidate protein" evidence="2">
        <location>
            <begin position="25"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="68"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP88304.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="353" mass="40125" checksum="20045A9B87CFFF75" modified="2012-04-18" version="2">MRLTASSALCLSFFIFQLADVTQGMEALRALRASSSSSRSAEASSEAAASSRALEYWIPPPTSSRVKEELAKSQDRIFPSSSSRPANLPPLKPQKAVEDPNSLVEHYEKAKFNPGALPSHDPRRVFIDEMKSEFIPEYKVHLIQRMFKNILENPGVTDDEIKHWFEVLAYVIRKTREVRAGSAESHLAVSALYGLNSLRMRLPERQALLTHIDALSVPLSRDIQQLPQDGILQLRWERELVYPSLGFGPELANRETFEKIFRNDRLISSAVSTSVKRSDKPLETLADEFRSSSAHKRVAILAVFYHQLVDSRKVKQVKSLFEQIERTRNLLPHERALIDFIRRKVKLPLPTQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3KXM0</accession>
    <name>E3KXM0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_14923</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178319">
        <property type="protein sequence ID" value="EFP89082.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333501.2">
        <property type="nucleotide sequence ID" value="XM_003333453.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KXM0"/>
    <dbReference type="STRING" id="418459.E3KXM0"/>
    <dbReference type="GeneID" id="10546551"/>
    <dbReference type="KEGG" id="pgr:PGTG_14923"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14923"/>
    <dbReference type="HOGENOM" id="CLU_012390_7_2_1"/>
    <dbReference type="InParanoid" id="E3KXM0"/>
    <dbReference type="OrthoDB" id="2503177at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125">
        <property type="entry name" value="F21J9.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125:SF3">
        <property type="entry name" value="NO-APICAL-MERISTEM-ASSOCIATED CARBOXY-TERMINAL DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="No apical meristem-associated C-terminal" evidence="2">
        <location>
            <begin position="141"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="24"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP89082.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="312" mass="35103" checksum="51CB6882702459CD" modified="2012-04-18" version="2">MANNNEENDNDENVGIDTPGAPKESQDKSGEEKKKAPNYTEPEDYELCRAWVQVSEDPAVGTNQEGSTFWQRVWTVYREAVPSPKRPVASLKKRWSNNLQPAINKFRGIVHQVEGLNESGASVEDQLNRALRLYTQDQETHFKHLRCYNLLVKSPKWNSYCRDSNQKETGKKKRARSPSSEAPQSQLASTPAPSDPVSEFEGTGSTPSILERPIGKKKAKMINQLAAKDQTWKDEVTAAHKQIAMESKRLNNIFAHDSTSIDRISAHGTTASELAIMTTDLKGLDDEQKEYFKLKRASILQSLRNEQNSSNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>E3KYR2</accession>
    <name>E3KYR2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Adenosinetriphosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_15300</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178322">
        <property type="protein sequence ID" value="EFP89458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333877.1">
        <property type="nucleotide sequence ID" value="XM_003333829.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KYR2"/>
    <dbReference type="FunCoup" id="E3KYR2">
        <property type="interactions" value="769"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KYR2"/>
    <dbReference type="GeneID" id="10547345"/>
    <dbReference type="KEGG" id="pgr:PGTG_15300"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15300"/>
    <dbReference type="eggNOG" id="KOG0733">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000688_8_1_1"/>
    <dbReference type="InParanoid" id="E3KYR2"/>
    <dbReference type="OMA" id="GLWSTHR"/>
    <dbReference type="OrthoDB" id="27435at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990275">
        <property type="term" value="F:preribosome binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd19518">
        <property type="entry name" value="RecA-like_NVL_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19530">
        <property type="entry name" value="RecA-like_NVL_r2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000018">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000365">
        <property type="entry name" value="Ribosome biogenesis ATPase RIX7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="174"/>
            <end position="314"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="511"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="27"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="69"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="767"/>
            <end position="795"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="117"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="5">
        <location>
            <begin position="783"/>
            <end position="795"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP89458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="795" mass="87410" checksum="76222551BD532427" modified="2011-01-11" version="1">MNSTPISTGNLLNRQIMGAYRKHHISLNSTPLPTNTTTTTEAATATTSTTTPTTINLTDELTTNQEKLTIDHSHSEHPTTSSTSKRKLNSTHENLNRITNQSTHSPTTIKRTKSAFSNSKQSGNPSSSSLIKDTTPSVTLSDLGGIDGTVERLLELIGLPILHPEIFEFTGLKPIRGLLLCGPPGCGKTMLANAIANQLGVRLINVSSTSIVSGMSGESEKAIRDIFEQATKQAPCLLFIDEIDAITPKRETAQREMERRIVAQLLTCLDDLSLEKTDGKPVIVIGATNRPDSLDPALRRGGRFDHEILMGVPDEAAREQILRVLCSKLKLVDSFDYKQLARSTPGYVGADLTALTASAGVIAIKRIFEPDTKPDKLPDDNMIIDLTDSPAKEDLLVVETDVPNQEQEAPPADIFENLPSHLLSLPIINFLRSNPRKLNSQQLDRIRINQLDFERALNELQPSCKREGFSTIPDTTWSQIGAMHSVRDELNISIIQPIRYPEMFEKIGISSSFGVLLWGPPGCGKTLVAKAVANESQANFISVKGPELLNKYVGESEKAVRQVFIRARASAPCIIFFDELDALVPRRDDSLSESSSRVVNTLLTELDGLEPRKQIYVIGATNRPDVMDPAMVRPGRLDKMIFIDLPDRQDRWEIFKTLSSKLTFDSPASEAIQKLIQDDPRCAGYSGADIGGLIREAALIQLRKQIRNRTLLDPTGDRPHHLETQTTQEESLGIADFEEALAKVRPSVSSSQHKRYRALHSKFNGVSAGTLKVQDQDDDPSCKPRPAPCPPPLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3KYT6</accession>
    <name>E3KYT6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Trafficking protein particle complex subunit 33</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_15324</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the TRAPP small subunits family. BET3 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178322">
        <property type="protein sequence ID" value="EFP89482.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333901.2">
        <property type="nucleotide sequence ID" value="XM_003333853.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KYT6"/>
    <dbReference type="FunCoup" id="E3KYT6">
        <property type="interactions" value="18"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KYT6"/>
    <dbReference type="GeneID" id="10547369"/>
    <dbReference type="KEGG" id="pgr:PGTG_15324"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15324"/>
    <dbReference type="HOGENOM" id="CLU_076409_0_0_1"/>
    <dbReference type="InParanoid" id="E3KYT6"/>
    <dbReference type="OrthoDB" id="941624at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005802">
        <property type="term" value="C:trans-Golgi network"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14944">
        <property type="entry name" value="TRAPPC6A_Trs33"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007194">
        <property type="entry name" value="TRAPP_component"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037992">
        <property type="entry name" value="TRAPPC6/Trs33"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12817:SF0">
        <property type="entry name" value="GEO08327P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12817">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04051">
        <property type="entry name" value="TRAPP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="217"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="222"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP89482.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="236" mass="26600" checksum="41FE999F0546A856" modified="2012-04-18" version="2">MAPSANINLYRPPSQVDAHLNDYFLNEMIHSISESAAHSLKTSSDAKRAYQEKKRLQLGGTGIKEDQEPSRITIQTQKTELDEAHRIRMDAAGYKVGWSLAERLSRDKPRISKAAPDPLEVIKFICKDIWTAMYNKQVDNLRTNHRGIYVIQDHSYRAFLRVSVPKGYESEMDEICRKMVIFPSAVIRGALMNLGIQSIVSVEIAIPQCTFQIRTAITTPNPNTPPGNPPNPVKQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3L0Q7</accession>
    <name>E3L0Q7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">N-acetyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_15959</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acetyltransferase family. GNAT subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178329">
        <property type="protein sequence ID" value="EFP90111.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334530.2">
        <property type="nucleotide sequence ID" value="XM_003334482.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L0Q7"/>
    <dbReference type="FunCoup" id="E3L0Q7">
        <property type="interactions" value="233"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L0Q7"/>
    <dbReference type="GeneID" id="10546089"/>
    <dbReference type="KEGG" id="pgr:PGTG_15959"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15959"/>
    <dbReference type="eggNOG" id="KOG4135">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073102_0_0_1"/>
    <dbReference type="InParanoid" id="E3L0Q7"/>
    <dbReference type="OrthoDB" id="5043642at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008080">
        <property type="term" value="F:N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000289">
        <property type="entry name" value="Predicted protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039135">
        <property type="entry name" value="NAT9-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13256:SF16">
        <property type="entry name" value="ALPHA_BETA-TUBULIN-N-ACETYLTRANSFERASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13256">
        <property type="entry name" value="N-ACETYLTRANSFERASE 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13302">
        <property type="entry name" value="Acetyltransf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="4">
        <location>
            <begin position="13"/>
            <end position="198"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP90111.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="270" mass="31027" checksum="843991AC68F96242" modified="2012-04-18" version="2">MRVNEQTAIIGPRVVLVPYRKEHVPQYHEWMSDQELRESTASEPLSLQEEYEMCERWALDQDKLTFIIFARNPCLSRTSDQPAGYKSYSDYLGQMIGDVNLFISPDESSDSTAAETAQSSHIYKGELEIMIASAQHRKLGLATEALQLFISYIQIISLLQSHSLVGSTSYFPTLSFFFVKIAFSNTISQGLFEKLGFEKKITNKIFEEHEYRLNFSESAITITIDRKNSLKQSEETDEILDDQAHREIRVYESKWKDNNPIQMAVVPCPM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3L186</accession>
    <name>E3L186_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16307</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178331">
        <property type="protein sequence ID" value="EFP90281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334700.1">
        <property type="nucleotide sequence ID" value="XM_003334652.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L186"/>
    <dbReference type="GeneID" id="10530175"/>
    <dbReference type="KEGG" id="pgr:PGTG_16307"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16307"/>
    <dbReference type="HOGENOM" id="CLU_126701_0_0_1"/>
    <dbReference type="InParanoid" id="E3L186"/>
    <dbReference type="OMA" id="DITWYRS"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="165" mass="18565" checksum="A4A9329F9880C2DD" modified="2011-01-11" version="1">MSPTTSTDSSRVLCPETQSPQTARPEVTTVMVKTPRVAAISLDYVLYIEPAKNGPLSRRQGLSPIKWEKIVPSPRPSPMEADIVSFTWPQFQTEAILHLANQREALQAFLFKNLDEGNLLWLAYIKDHHDYGLAVQIHGATDFLNFSNAAYNAFTRGNFRALDFQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3L252</accession>
    <name>E3L252_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein phosphatase methylesterase 1</fullName>
            <ecNumber evidence="2">3.1.1.89</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_16653</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>[phosphatase 2A protein]-C-terminal L-leucine methyl ester + H2O = [phosphatase 2A protein]-C-terminal L-leucine + methanol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:48548"/>
            <dbReference type="Rhea" id="RHEA-COMP:12134"/>
            <dbReference type="Rhea" id="RHEA-COMP:12135"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:90516"/>
            <dbReference type="ChEBI" id="CHEBI:90517"/>
            <dbReference type="EC" id="3.1.1.89"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the AB hydrolase superfamily.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.89" evidence="2"/>
    <dbReference type="EMBL" id="DS178335">
        <property type="protein sequence ID" value="EFP90627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335046.1">
        <property type="nucleotide sequence ID" value="XM_003334998.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L252"/>
    <dbReference type="STRING" id="418459.E3L252"/>
    <dbReference type="GeneID" id="10530527"/>
    <dbReference type="KEGG" id="pgr:PGTG_16653"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16653"/>
    <dbReference type="eggNOG" id="KOG2564">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_024818_1_0_1"/>
    <dbReference type="InParanoid" id="E3L252"/>
    <dbReference type="OMA" id="MHARISM"/>
    <dbReference type="OrthoDB" id="194865at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051723">
        <property type="term" value="F:protein methylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1820:FF:000836">
        <property type="entry name" value="Protein phosphatase methylesterase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1820">
        <property type="entry name" value="alpha/beta hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000073">
        <property type="entry name" value="AB_hydrolase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029058">
        <property type="entry name" value="AB_hydrolase_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016812">
        <property type="entry name" value="PPase_methylesterase_euk"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14189:SF0">
        <property type="entry name" value="PROTEIN PHOSPHATASE METHYLESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14189">
        <property type="entry name" value="PROTEIN PHOSPHATASE METHYLESTERASE-1 RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12697">
        <property type="entry name" value="Abhydrolase_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53474">
        <property type="entry name" value="alpha/beta-Hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0719">Serine esterase</keyword>
    <feature type="domain" description="AB hydrolase-1" evidence="7">
        <location>
            <begin position="44"/>
            <end position="158"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF12697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP90627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="182" mass="19020" checksum="6F334262DCA49560" modified="2011-01-11" version="1">MDTIDQKYAPLSAADCFQEALLVTVGASKLFRVYSNPGKSPVLFVFHHGAGYSALSAACLARELREQGGGQYGFLSFDCRAHGASQVESEDSGPPDLSLSTLANDLVDLLKTIYPDKTLAPAFVLVGHSMGGAVVTEACAGIQTHVGKVLGLVILDVVEGSALSALISMMPLINSRPSSFSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3L4L2</accession>
    <name>E3L4L2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17737</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178347">
        <property type="protein sequence ID" value="EFP91487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335906.1">
        <property type="nucleotide sequence ID" value="XM_003335858.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L4L2"/>
    <dbReference type="GeneID" id="10529488"/>
    <dbReference type="KEGG" id="pgr:PGTG_17737"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17737"/>
    <dbReference type="HOGENOM" id="CLU_1949892_0_0_1"/>
    <dbReference type="InParanoid" id="E3L4L2"/>
    <dbReference type="OrthoDB" id="2510371at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="17"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="129" mass="14513" checksum="AC80FA4B36608A0D" modified="2011-01-11" version="1">MSKSSQDNTFKNQTPQAEMTLKNAGVPLETEPEAPAPDIQPATTTQPTPLEEVEYIYDVVSAMKHALIQDHNSELFLETPNFNKVHEEYIKALEVFLERVSAIIKETLEAGNHFIYDSLDIPQISSPKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3L4X2</accession>
    <name>E3L4X2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription initiation factor IIF subunit alpha</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_17651</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the TFIIF alpha subunit family.</text>
    </comment>
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        <property type="protein sequence ID" value="EFP91597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336016.1">
        <property type="nucleotide sequence ID" value="XM_003335968.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L4X2"/>
    <dbReference type="FunCoup" id="E3L4X2">
        <property type="interactions" value="57"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L4X2"/>
    <dbReference type="GeneID" id="10531534"/>
    <dbReference type="KEGG" id="pgr:PGTG_17651"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17651"/>
    <dbReference type="eggNOG" id="KOG2393">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_382229_0_0_1"/>
    <dbReference type="InParanoid" id="E3L4X2"/>
    <dbReference type="OMA" id="IPIMTTK"/>
    <dbReference type="OrthoDB" id="2504792at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005674">
        <property type="term" value="C:transcription factor TFIIF complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016251">
        <property type="term" value="F:RNA polymerase II general transcription initiation factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001096">
        <property type="term" value="F:TFIIF-class transcription factor complex binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032968">
        <property type="term" value="P:positive regulation of transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008851">
        <property type="entry name" value="TFIIF-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011039">
        <property type="entry name" value="TFIIF_interaction"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13011:SF0">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13011">
        <property type="entry name" value="TFIIF-ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05793">
        <property type="entry name" value="TFIIF_alpha"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50916">
        <property type="entry name" value="Rap30/74 interaction domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="52"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="259"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="349"/>
            <end position="651"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="72"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="259"/>
            <end position="273"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="284"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="294"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="370"/>
            <end position="387"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="402"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="9">
        <location>
            <begin position="474"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
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            <begin position="503"/>
            <end position="531"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="541"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="580"/>
            <end position="593"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="600"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU366044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP91597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="724" mass="79335" checksum="AB0D3C5E3ED6F7A6" modified="2011-01-11" version="1">MSQARTTSKKPNQEPTPMETDPPKPPPTEAKYVSYTLLAAHDPQLKYNILKFAPSSNRKIEVDSFVQPIKLNRKDPWAIRKKAEEEKKRSTEAQEENASKTGENDNNDDENDNEMKVDQDNKASSTSTKQVRDDSVIGPAPASQPKKNQPFKKKTKQVFIAADQSARMLKKEEYQSWLLEDGSPNGERWVGRYESAGAAAGSSASASTSTSTNNDASNYVLFRMDPSGEAFQVIPCHRFYRFTQRPNYDTLGADEAEAAYEKMQKPTTKEDVGRWFMRRRGANPSSSGSSQPSKTLDNKTSGNVKHEGDVKPTSFFDHSKVSFGTSNKVRSQVEIPSFINGRRNKFTAVQGSNSRLDDDEDRKPNFGQEGDFDEVDYDEQFDDDEEGAGNLNDEAMEEDELKELAEKMKREMLAAGKAGDDEREKEDVDMAKDDLFGEREDLTKEGKAVKKLLMRKGEDNVYDSDDEVRNPYASEEDESDFEEVVPPPPNGPSPTDAKDPASRSGTPTPGSQPAGTSKSIATKGPVTQASNLKGDKPQSRPPSRSGTPSLPNHSRTNSASHPVHGHGFNAGPANRSRATSPQLPSSQLASSSAGPRSRAVSPVLSSSLKPSSPISANASTTNSNGKRKQHPNGAIASKEKKLNSGPTPPGRISSTISAAPDQESGVITKEQVVTLLKIRPLTTKELLAHFKQRLKEESNKVIIFAIIREIANIHEGLLVLKDGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3L5X5</accession>
    <name>E3L5X5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_18401</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178355">
        <property type="protein sequence ID" value="EFP91950.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336369.2">
        <property type="nucleotide sequence ID" value="XM_003336321.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L5X5"/>
    <dbReference type="GeneID" id="10531902"/>
    <dbReference type="KEGG" id="pgr:PGTG_18401"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18401"/>
    <dbReference type="HOGENOM" id="CLU_1210320_0_0_1"/>
    <dbReference type="InParanoid" id="E3L5X5"/>
    <dbReference type="OrthoDB" id="10272167at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="123"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="221"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP91950.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="240" mass="27019" checksum="91BF15638DF949F4" modified="2012-04-18" version="2">MAIYRINTLNVFILIIHSSSLIPYFFHHCIARPLYHGAPLTWENTRAPLTDAEMDEVLNFDCNDLPMFFDSDNLSGLAVPEPTKHSFPNDQLYPLPSSLDPHQLPTDFYQYVYDSAPSLSGIETPNDLYPRDSNTYEATSIPSAKSPNCNESNNVSQHGPSQPNDGLPFSQLQDALEGFRRIPITLPDYERCLKEHGYSSSKLGSLPLKWIHPLSNHDQSVQGKLFSNQNAEQFGYPSSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3L7I9</accession>
    <name>E3L7I9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_18512</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178366">
        <property type="protein sequence ID" value="EFP92514.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336933.2">
        <property type="nucleotide sequence ID" value="XM_003336885.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L7I9"/>
    <dbReference type="GeneID" id="10538509"/>
    <dbReference type="KEGG" id="pgr:PGTG_18512"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18512"/>
    <dbReference type="HOGENOM" id="CLU_104741_0_0_1"/>
    <dbReference type="InParanoid" id="E3L7I9"/>
    <dbReference type="OrthoDB" id="2507283at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP92514.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="152" mass="17187" checksum="8AFD48884DBFFD49" modified="2012-04-18" version="2">MNAENFHNEKHKVMWITGYFCKPDGKLGDNCASYVWWRGLMAKNANHQNLDPNTALSKAPFLLEELLSGEAFLVAIETTFSNHKGEEEACKSLRVMKQGNTSIEEFNVSFNSLLYAVDLSEASKCDLYNKAINPKIIQLRILRGGWNGVTVW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8B7</accession>
    <name>E3L8B7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Enoyl-CoA hydratase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18571</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178373">
        <property type="protein sequence ID" value="EFP92792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337211.1">
        <property type="nucleotide sequence ID" value="XM_003337163.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L8B7"/>
    <dbReference type="GeneID" id="10542505"/>
    <dbReference type="KEGG" id="pgr:PGTG_18571"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18571"/>
    <dbReference type="eggNOG" id="ENOG502QS1J">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009834_3_1_1"/>
    <dbReference type="InParanoid" id="E3L8B7"/>
    <dbReference type="OMA" id="LWIIELH"/>
    <dbReference type="OrthoDB" id="1696280at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004165">
        <property type="term" value="F:delta(3)-delta(2)-enoyl-CoA isomerase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006635">
        <property type="term" value="P:fatty acid beta-oxidation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd06558">
        <property type="entry name" value="crotonase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.226.10:FF:000113">
        <property type="entry name" value="Enoyl-CoA hydratase/isomerase family protein (AFU_orthologue AFUA_2G14850)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001753">
        <property type="entry name" value="Enoyl-CoA_hydra/iso"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941">
        <property type="entry name" value="ENOYL-COA HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11941:SF75">
        <property type="entry name" value="ENOYL-COA HYDRATASE_ISOMERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00378">
        <property type="entry name" value="ECH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="267"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="296" mass="31977" checksum="4A0F481CE2D4E1F9" modified="2011-01-11" version="1">MASVTPLPSTTRYPCSTLTKPADYPGVFLLELHSPPDNRMTEHFITSSLIAPLLDVEREYRASLLGLLTTPPPGDSQGWKTPLTPGQYALVTCGARDKNRIYSNGLDLNEALALDHFFQHVLNPLYAKLLAFPIPTVAAINGHAFAGGFCLALVHDFRIMKDNEQKGRALMAMNEAEFGAPIPLGLISIVETKFPSTTLAAKCLAEAHRFVAKESLELGIVDALAPESEVVNNSLRLAFEKSIRASTGVYGMIKESVYRKTLETLGSSGSGNADVKSVHTSRLKQLSGHKSGASKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3L968</accession>
    <name>E3L968_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Retrotransposon gag domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19373</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178381">
        <property type="protein sequence ID" value="EFP93093.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337512.2">
        <property type="nucleotide sequence ID" value="XM_003337464.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L968"/>
    <dbReference type="GeneID" id="10543158"/>
    <dbReference type="KEGG" id="pgr:PGTG_19373"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19373"/>
    <dbReference type="HOGENOM" id="CLU_018307_4_1_1"/>
    <dbReference type="InParanoid" id="E3L968"/>
    <dbReference type="OrthoDB" id="2505542at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="36"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="126"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93093.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="436" mass="47259" checksum="6707F6F1E66FA352" modified="2012-04-18" version="2">MSTRRTTSSKDLFTPLSDPDAAFHVANAEKRRLKAARSEETAAINVPAETRAESTASIKEPAELPLHASQPISRPSMPITASLPNPPTVFPPPSPFLTLSTMQTASTSSTTDPPSDVARRPIALASPSLTTSTPTDLSNPGPKPPGNGQTTGSVVLGNGETTRVQERTTVGPGERPFVNPGASLSQSLADREAAVAERIANADQIARLEEALLLLSVKQEGTKPPPRPTDGGVDLQRFRIADGPVYQGPFQTVEPFLNWVRSLEIFFETKGVSLDSDKIRIAGGLVCETNTLSFYANNAATHSRGSWKDFKTKLFDFALPALWDSKLREQIVRLDMADSKSFLTYSTRARTLQSLVNFNQKMVSDFALAEAVTFGMTAALKAKVHDFELLLVSLFVYGGFERRATGLFDSIPKRCGTFQAKEKSLEGKRTTTQGEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3L989</accession>
    <name>E3L989_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19219</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178382">
        <property type="protein sequence ID" value="EFP93114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337533.1">
        <property type="nucleotide sequence ID" value="XM_003337485.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L989"/>
    <dbReference type="GeneID" id="10543179"/>
    <dbReference type="KEGG" id="pgr:PGTG_19219"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19219"/>
    <dbReference type="HOGENOM" id="CLU_2251385_0_0_1"/>
    <dbReference type="InParanoid" id="E3L989"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="104" mass="12387" checksum="807C110C393701EA" modified="2011-01-11" version="1">MSSRRQKRRGKRFPGFNRSEHDEASKSRRRRSTHVGNAKKFHIWNKCLKNDDTFDMRLRFGIKNPDVHKYEKDSRMQFSDPNSYSSTKPLHPAAYFIVRGTEIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3L9A0</accession>
    <name>E3L9A0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_19230</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178382">
        <property type="protein sequence ID" value="EFP93125.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337544.2">
        <property type="nucleotide sequence ID" value="XM_003337496.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L9A0"/>
    <dbReference type="GeneID" id="10543190"/>
    <dbReference type="KEGG" id="pgr:PGTG_19230"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19230"/>
    <dbReference type="HOGENOM" id="CLU_2741242_0_0_1"/>
    <dbReference type="InParanoid" id="E3L9A0"/>
    <dbReference type="OrthoDB" id="10270597at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP93125.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="76" mass="8554" checksum="E359BD8A12A40AAE" modified="2012-04-18" version="2">MDSNQASLIGSDWGFQKEDLSRTLYLLDCYVPATRLLVYYHSMVVALEATADKKSVIQNDHIAGPLDSLSRPFETF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3L9G5</accession>
    <name>E3L9G5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19429</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178385">
        <property type="protein sequence ID" value="EFP93190.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337609.2">
        <property type="nucleotide sequence ID" value="XM_003337561.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L9G5"/>
    <dbReference type="GeneID" id="10543340"/>
    <dbReference type="KEGG" id="pgr:PGTG_19429"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19429"/>
    <dbReference type="HOGENOM" id="CLU_044887_0_0_1"/>
    <dbReference type="InParanoid" id="E3L9G5"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="125"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="209"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93190.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="331" mass="36782" checksum="9D7F5271BEE006F6" modified="2012-04-18" version="2">MAISQLIGSIAAQAFIFIQIKTSHSSDYALKSPLASPFPIFVVAATIALFTWLSSGFTHFMVAVVKVLDALRQGSSAWDQLKVPSTSSEEKLHLTTQLIQVVSEAKNFTTDVNIRSKNLINCFHLVQCVLLIIICTTSLVFIVSFWVLVHKFRRRHRQSDKASSRSIYLRRWGKRNNSRNASGEQSTTSSNLNPSFIDVVKSDRQFLHLALRAISIIIAMLTTISLLLLGIIRTADVVKYPYWRGLSTWLTTVSGTWAAIPIAWQCWRLYQEQSGGASQSLSNSNGTTLIGVSGNKNADPGRPTQDGAETIEIKIELDPKAIHPTETYEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>E3L9K1</accession>
    <name>E3L9K1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_19172</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178386">
        <property type="protein sequence ID" value="EFP93226.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337645.2">
        <property type="nucleotide sequence ID" value="XM_003337597.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L9K1"/>
    <dbReference type="GeneID" id="10543377"/>
    <dbReference type="KEGG" id="pgr:PGTG_19172"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19172"/>
    <dbReference type="HOGENOM" id="CLU_2543681_0_0_1"/>
    <dbReference type="InParanoid" id="E3L9K1"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP93226.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="83" mass="9151" checksum="E33F626D5E70BFA4" modified="2012-04-18" version="2">MAKWVQMAPDNLRTERFKKLLLQLLKCLGIGKLEQAASSFEPTQLHTQVRPCSNLANVPPESEEVANRGQSPGDLVGFCRLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3L9X9</accession>
    <name>E3L9X9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_19087</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178390">
        <property type="protein sequence ID" value="EFP93354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337773.1">
        <property type="nucleotide sequence ID" value="XM_003337725.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L9X9"/>
    <dbReference type="GeneID" id="10543700"/>
    <dbReference type="KEGG" id="pgr:PGTG_19087"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19087"/>
    <dbReference type="HOGENOM" id="CLU_963586_0_0_1"/>
    <dbReference type="InParanoid" id="E3L9X9"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP93354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="278" mass="30726" checksum="4E616118295964E6" modified="2011-01-11" version="1">MAGHSLIPSTNPSSKPPARSTKNFKCLYCFQKGHTISRCHIVSYDQQKGLIKRIGSEFWLSDSSPILVDPWRPIKEIVNTFSATHNHQPVILPPPSTDFSYSLSSTAATKESTIEEDLGKVILSSAQTTEETNRQVVDDLPSPGSIKVRLNGSNCEGLLYPGWLFNFISEDLYLEVHPQDILLPGVCDDDIKITGIALASLSLEGNFETISFLFTPRIRPIILGKPFLNDFLADIDLSNPLAPKLSILDSTGAEHAFPISPDKRNQAYLNIFQNLVSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3LB68</accession>
    <name>E3LB68_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Threonylcarbamoyl-AMP synthase</fullName>
            <ecNumber evidence="3">2.7.7.87</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">L-threonylcarbamoyladenylate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGTG_19838</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Likely catalyzes the conversion of L-threonine, HCO(3)(-)/CO(2) and ATP to give threonylcarbamoyl-AMP (TC-AMP) as the acyladenylate intermediate, with the release of diphosphate. Required for normal translation, by ensuring translation fidelity at the level of codon recognition, appropriate translation initiation selection and maintenance of reading frame. Also involved in telomere replication. Binds to single-stranded telomeric (ssTG) DNA and positively regulates telomere length.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-threonine + hydrogencarbonate + ATP = L-threonylcarbamoyladenylate + diphosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:36407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="EC" id="2.7.7.87"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SUA5 family.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.87" evidence="3"/>
    <dbReference type="EMBL" id="DS178409">
        <property type="protein sequence ID" value="EFP93793.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338212.2">
        <property type="nucleotide sequence ID" value="XM_003338164.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LB68"/>
    <dbReference type="FunCoup" id="E3LB68">
        <property type="interactions" value="242"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3LB68"/>
    <dbReference type="GeneID" id="10535243"/>
    <dbReference type="KEGG" id="pgr:PGTG_19838"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19838"/>
    <dbReference type="HOGENOM" id="CLU_031397_0_0_1"/>
    <dbReference type="InParanoid" id="E3LB68"/>
    <dbReference type="OrthoDB" id="412787at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061710">
        <property type="term" value="F:L-threonylcarbamoyladenylate synthase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002949">
        <property type="term" value="P:tRNA threonylcarbamoyladenosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.11030:FF:000005">
        <property type="entry name" value="Threonylcarbamoyl-AMP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.870.10:FF:000008">
        <property type="entry name" value="Threonylcarbamoyl-AMP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.10">
        <property type="entry name" value="DHBP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11030">
        <property type="entry name" value="Threonylcarbamoyl-AMP synthase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="Sua5-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038385">
        <property type="entry name" value="Sua5/YwlC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005145">
        <property type="entry name" value="Sua5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050156">
        <property type="entry name" value="TC-AMP_synthase_SUA5"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00057">
        <property type="entry name" value="L-threonylcarbamoyladenylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490">
        <property type="entry name" value="SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490:SF16">
        <property type="entry name" value="THREONYLCARBAMOYL-AMP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03481">
        <property type="entry name" value="Sua5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="YrdC/RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="YrdC-like" evidence="14">
        <location>
            <begin position="99"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EFP93793.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="491" mass="53069" checksum="17C7710FA2D1CA2E" modified="2012-04-18" version="2">MQGVCSCYYPPGVAVSLVPRYFLAPIKQSVRVSFRKSNHLRSFRFAYMTSTAESQSAGTTLDGLSSQDQTTNVIQCNRDSIVFTDKNEGKPLFNCHQTATGIQAAAKVIQSEDLVAFPTETVYGLGASALSSTAVSKIFKAKGRPSDNPLIVHVSSLNLLKTFVPPDWNFPATYQALIERFWPGPLTLLVPTTSPVQPNCPISNLVTCGLPTVAVRMPAHSISRALIAESGVPIAAPSANLSGRPSPTTAQHVERDMKTKIPMIIDGGACQVGVESTVVDGLNPDGRVRILRLGGLSVEDIEACLIEAGLNLSDGKPILAGVYNQDYRDKELEAKPTTPGMKYKHYAPHAKVVILDPVLVPNSDSDQQESLRGVDELVSGICSEETDSSKGLRIGLMLIDDSPLHKSFIACAQSEKYGHHQFCYSNLGLESQPAISAQRLFAALRYLDEELRVDIIYVERLSEVGLGATVMERLRKASGQSSPLPVRISTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3LC33</accession>
    <name>E3LC33_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptidase A2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_20145</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178442">
        <property type="protein sequence ID" value="EFP94108.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338527.2">
        <property type="nucleotide sequence ID" value="XM_003338479.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LC33"/>
    <dbReference type="GeneID" id="10535887"/>
    <dbReference type="KEGG" id="pgr:PGTG_20145"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20145"/>
    <dbReference type="HOGENOM" id="CLU_377733_0_0_1"/>
    <dbReference type="InParanoid" id="E3LC33"/>
    <dbReference type="OrthoDB" id="2506366at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05483">
        <property type="entry name" value="retropepsin_like_bacteria"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.70.10">
        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001995">
        <property type="entry name" value="Peptidase_A2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021109">
        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034122">
        <property type="entry name" value="Retropepsin-like_bacterial"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13975">
        <property type="entry name" value="gag-asp_proteas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50630">
        <property type="entry name" value="Acid proteases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50175">
        <property type="entry name" value="ASP_PROT_RETROV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A2" evidence="3">
        <location>
            <begin position="484"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="312"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="706"/>
            <end position="734"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="146"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="317"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="359"/>
            <end position="368"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="380"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP94108.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="734" mass="84087" checksum="4E3E643CEF19E758" modified="2012-04-18" version="2">MEGHETGDWELLKKELTRKWGRATPFRKYREDAIPRLVQKAQEGQGIKTRLEYRKFVGELEEMTDYFTRMGYSHLNPDSGNPLWSALSAELKKEVTKELAHAKKLKKTKDGRNIIPELDILKEYVELALIIIYFDEDEPQTTSSDSTKKKVKIQDPSETEELKETIKKLNNSLEYQARAAPPHLNRPSSPGFGDTRPHFTAVECFYCKEKHMLSQCEAYTQDMQDRRMFRYQGVYYYPNRQPIVVDKDCSVKEMVTRFHDEQSNSRTENTAVQESTSAVIEVEEWGSWLPPQANISEEELQNNIGFGLRKSQRIQDKNPQASSQPMPVKTQESVSKTPPPNQEAIKPPTRRKSFPGSWLEEEELEEEESSKQKSTGPSRENPKQSREELRAKEITIEPLDKSIRNKFFKQTYTLTLEEIVKIAPQFLQGLQESRPEYDVSEKKANNVELHCSTGLGTSENEEERGLTYACPVGMVDMTINKRKIRTLVDTGAEMNIIPDTLADQLGLVTTEIFMRLKGIGGHVTPIVGLAENIPVNVLELSHQKGEVLSFPDSARRRVCIPICLPDSPGWHKEPPALRQNCSFQVVDWNLFDQIDTVGPQDKNTTVIEWDQLEGLEETLFSSVVVKSENSQSKEDNFLGSYLYGFQTQDELWEDDPWNMHRLEAVDWEKELGAATLTPEEQKQTDKQLADAEAAWAAEPIDVDSKTYSTELTYPRRRETHKTGLKSYPEGSRTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>E3NX73</accession>
    <name>E3NX73_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_20055</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS989920">
        <property type="protein sequence ID" value="EFP94172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338591.1">
        <property type="nucleotide sequence ID" value="XM_003338543.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NX73"/>
    <dbReference type="GeneID" id="10535944"/>
    <dbReference type="KEGG" id="pgr:PGTG_20055"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20055"/>
    <dbReference type="HOGENOM" id="CLU_2293053_0_0_1"/>
    <dbReference type="InParanoid" id="E3NX73"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP94172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="101" mass="11035" checksum="36E1884E1A2366B7" modified="2011-01-11" version="1">MAISCFPPVERSWELTPKPVKGFGGSTPTLWAIQGLGVGSPLFGSCLTKPLNDLEQIKAQGCQIGFPQKRCLGVRTSRKAKSSKISFLLIPINNDHHKFPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3NY83</accession>
    <name>E3NY83_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">deoxyhypusine synthase</fullName>
            <ecNumber evidence="6">2.5.1.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_20488</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>[eIF5A protein]-L-lysine + spermidine = [eIF5A protein]-deoxyhypusine + propane-1,3-diamine</text>
            <dbReference type="Rhea" id="RHEA:33299"/>
            <dbReference type="Rhea" id="RHEA-COMP:10143"/>
            <dbReference type="Rhea" id="RHEA-COMP:10144"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57484"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:82657"/>
            <dbReference type="EC" id="2.5.1.46"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; eIF5A hypusination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the deoxyhypusine synthase family.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.46" evidence="6"/>
    <dbReference type="EMBL" id="DS990051">
        <property type="protein sequence ID" value="EFP94532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003339486.1">
        <property type="nucleotide sequence ID" value="XM_003339438.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NY83"/>
    <dbReference type="STRING" id="418459.E3NY83"/>
    <dbReference type="GeneID" id="10618344"/>
    <dbReference type="KEGG" id="pgr:PGTG_20488"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20488"/>
    <dbReference type="HOGENOM" id="CLU_039781_3_1_1"/>
    <dbReference type="InParanoid" id="E3NY83"/>
    <dbReference type="OrthoDB" id="294378at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034038">
        <property type="term" value="F:deoxyhypusine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.910.10:FF:000010">
        <property type="entry name" value="Deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.910.10">
        <property type="entry name" value="Deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002773">
        <property type="entry name" value="Deoxyhypusine_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036982">
        <property type="entry name" value="Deoxyhypusine_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703:SF0">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01916">
        <property type="entry name" value="DS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP94532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="135" mass="14737" checksum="A08DC1EBFFE83DD9" modified="2011-01-11" version="1">MLCFHSIKTDPNPLVIDIVADIRRINELGRSAKKAGMVILGGGVCKHQIANAMLYRNGAEYSVYVNTGQEFDGSDSGARPDEAISWGKIKGDSESVKVFADATLVFPLIVAATWAKDHWKSKPETETTEPVESKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>H6QNT9</accession>
    <name>H6QNT9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BRCA2 OB1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_20647</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="5" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EHS62527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890844.1">
        <property type="nucleotide sequence ID" value="XM_003890795.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QNT9"/>
    <dbReference type="STRING" id="418459.H6QNT9"/>
    <dbReference type="GeneID" id="13542490"/>
    <dbReference type="KEGG" id="pgr:PGTG_20647"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20647"/>
    <dbReference type="HOGENOM" id="CLU_271491_0_0_1"/>
    <dbReference type="InParanoid" id="H6QNT9"/>
    <dbReference type="OrthoDB" id="21095at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000406">
        <property type="entry name" value="Breast and ovarian cancer susceptibility protein 2 truncated variant"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000579">
        <property type="entry name" value="Chromosome 8, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015525">
        <property type="entry name" value="BRCA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015252">
        <property type="entry name" value="BRCA2_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036315">
        <property type="entry name" value="BRCA2_hlx_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015187">
        <property type="entry name" value="BRCA2_OB_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11289:SF0">
        <property type="entry name" value="BREAST CANCER TYPE 2 SUSCEPTIBILITY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11289">
        <property type="entry name" value="BREAST CANCER TYPE 2 SUSCEPTIBILITY PROTEIN BRCA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09169">
        <property type="entry name" value="BRCA-2_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09103">
        <property type="entry name" value="BRCA-2_OB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81872">
        <property type="entry name" value="BRCA2 helical domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Breast cancer type 2 susceptibility protein helical" evidence="3">
        <location>
            <begin position="777"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="domain" description="BRCA2 OB1" evidence="2">
        <location>
            <begin position="831"/>
            <end position="964"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="167"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="370"/>
            <end position="423"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="480"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="611"/>
            <end position="632"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="912"/>
            <end position="936"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="27"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="93"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="184"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="270"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="331"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="399"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="484"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="914"/>
            <end position="923"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="924"/>
            <end position="936"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EHS62527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1241" mass="136843" checksum="3BD2C759AD64372C" modified="2012-04-18" version="1">MSAATSNTNDGRPTKRTRFSTSEQPEDGSRPRAGPVEHAEREWPEAEVSGSSQNPRSSEGNVLLQDHVESDPRPVDRQAPPLSTVIHHPLASVASQSQPDREQSLTPLTETRSEDRPLTAEVSENDLEISQYDIWSDCEQEELDALDETELLNSKILNTSQLATPATIQDQAKPSQAAPDLSNELVQGISQSESNASPDHQPSSAQQSINPSRSNSSPLHRQSNLDSSQSPVRIKRSVSSLFTTGTGEPFPMAIEQPITSPPFESTIFPPRRPSAPPNPPSITSQADDRTAQITCEVPESGPSKSKPRRALSSLFQTGAGQPFPMYDPTHEGSSSSAQNQDCPVFGFQTARGNTVAAPSKESMRKALDICNNDNLSPFPKPHYKEPDSPSPSHRAKMQKVNSQSVSPSKMSNNQTDDNQSQADMIPARTPQAKEFSPTGSSVMYPPITKFDSSKPSVGLFSTGAGAPIHIPISAVERARAHLDNPSQSSELHPLPTNESSSPLKDVSNLRPASQSNHRMTHDLLTGSINRQSNHHSISAKLHESLNAVVPKIPSQPTTAASLMSTPIRTLSNGISRPNLQNSPHEPISLPSVPHTTTAPVRRISNRMHIGLTPQSSSRRVSHESKGPEPRSFITPFKKKIEVPHAKPGQFNSLCTPARDSSNLYQCEETPSLDPSRRSVMYIDPPTPFSRQGSSGKPQSVFDLSTRSTRRKIPMVDWLASPKGYSARHLLSLAVPESVVFMTAKSAAIWSFNAFTEPFGAIHALGELLQAGCSINRCSPKWVANHWALIIWKLAAMVRWQPDCLSRVWNPSNAVQQLKYRYEREFVCGDRSALKRILDNDCPSSMPMCLVIVGIIRRPGPSSATKGIPVTTEILELSDGWYKIKAHLDPVLARVLKRGTLRVGFKLAISGASTEAPAGPSSKSSGEKKSTILKETDDGKEEEVRLYLEGNATSRARWPETLGFRPRPWVASLRSLSPDGGRIPLMDIVISKVFPPMFVDQEGRMGRWGVAEENMLQVAWEKEREKEAAKIAGEQEQEFEKIQQVADLVASIYAAKSDGKRRVSGDKRNGEEADSEEFAAFDADELCDDLIEDDASMEVMKQLSVTNLVQLRQSVQRRCDTFRSKAVDELQKTLNSRVPLRRTRQVQQLTFRDFQTFKTSARTPSGSPSRLVGQLSVQDLSLLPDRFFKEGARYWVHNIQPQRNARWDLSASSSSKNFKNELVEVPLSTRRDTTWRNISLDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>H6QNU2</accession>
    <name>H6QNU2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF8040 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20649</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EHS62529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890846.1">
        <property type="nucleotide sequence ID" value="XM_003890797.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QNU2"/>
    <dbReference type="STRING" id="418459.H6QNU2"/>
    <dbReference type="GeneID" id="13542485"/>
    <dbReference type="KEGG" id="pgr:PGTG_20649"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20649"/>
    <dbReference type="eggNOG" id="KOG4585">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_040082_1_0_1"/>
    <dbReference type="InParanoid" id="H6QNU2"/>
    <dbReference type="OrthoDB" id="2505412at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058353">
        <property type="entry name" value="DUF8040"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF85">
        <property type="entry name" value="GH03217P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26138">
        <property type="entry name" value="DUF8040"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF8040" evidence="1">
        <location>
            <begin position="96"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="265" mass="29986" checksum="5002E514BA28DF7B" modified="2012-04-18" version="1">MDIHSSNPPSLATQFTYFHSSDTSLQHLKQPTMDNNLLAMLAAAGASLVQLNNTIISIAAAGAAITYLGKMWNTYEPYYGDGGQARYTRFLLNEVRPELFREITRMERSTFNSLVEELKFNRLLKDGRSVCVEEQVLIFLDILCHNNAMRQTAVKFRRGLYTVTRYFGLVLDSLVNIYPNYVKFNMESCIQPDDISKNPRYKAFKNALGAINGVFIPATVPANIQSPWRNRKGFIAQNVLAAVNFNFEFVYVLAGWEGSAHDTRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>H6QNV5</accession>
    <name>H6QNV5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20619</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EHS62497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890814.1">
        <property type="nucleotide sequence ID" value="XM_003890765.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QNV5"/>
    <dbReference type="GeneID" id="13542542"/>
    <dbReference type="KEGG" id="pgr:PGTG_20619"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20619"/>
    <dbReference type="HOGENOM" id="CLU_035747_0_0_1"/>
    <dbReference type="InParanoid" id="H6QNV5"/>
    <dbReference type="OrthoDB" id="2506836at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="354"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="399"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="364"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="427"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="469" mass="53066" checksum="B029DE792460F7ED" modified="2012-04-18" version="1">MAPNQVQRAQNMDLDELIKLVASTAQYTRLTAENEVELNDAYMEYQRQLYLIAYKKKLAIAPCLQYVGEGINPRGASNYNNFCRYDLVASKVAQDKTMHVEDRMRELSRLWHLNDDTAKEQWKDPEFLKSLPNFTEVNKDASTSGTQKHKPPTFDADRWSNKIVADLRNLSRSSGIEGFLVVGSRGKDSSLNFQGGSHLGERFLDMYATEDDPVSNFMDFIKGFKVIQKITGSEPPPVVTKKKPRKPKPREIITPHDKGSRKKNIKFIREKLNAAIKLATHGTWTKGWPGTRTQWQLARLKVTLQVKQDNDLNVSPIHFCRRPGDMLEIPAQMVVAAIEEDWVQLIGPPAPSNDSVGCDIDSPANAEANAATTTNTATTTNTATTTVSVAQCAIEKLPIKQKKRKTGPATLKANPPKKKQKRASNQEGEEEEEEEEEEEEEEEDEDEEDEEEEEEEEEEEEEVVDEQDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPT4</accession>
    <name>H6QPT4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_20932</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EHS64321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890477.1">
        <property type="nucleotide sequence ID" value="XM_003890428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPT4"/>
    <dbReference type="GeneID" id="13542598"/>
    <dbReference type="KEGG" id="pgr:PGTG_20932"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20932"/>
    <dbReference type="HOGENOM" id="CLU_105965_0_0_1"/>
    <dbReference type="InParanoid" id="H6QPT4"/>
    <dbReference type="OrthoDB" id="10664534at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="236" mass="25952" checksum="EE11F310F2AFEC72" modified="2012-04-18" version="1">MTIQTGSLHTVRNRQLTPRRDYLCFSGVFKGELGPFLRARPNQGPLEMMKSSVYTSGKWKTGPGQTTINVTCAYHEDFALSVGGPQRYNGKITKMDDDSVLSFQLSDGETIENVGQERGRTQGVTIDGVGSIVESWTKEVEQGANSKTSFVVVEHDVNNNTVDVITVTYVFSENSVIMPPRVKFNVGDKILIVGQLESTGDGSDGLIIESKVVQYPFTAPWQCSPNMIAVEHLTRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQ81</accession>
    <name>H6QQ81_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21054</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EHS64796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890317.1">
        <property type="nucleotide sequence ID" value="XM_003890268.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQ81"/>
    <dbReference type="GeneID" id="13542114"/>
    <dbReference type="KEGG" id="pgr:PGTG_21054"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21054"/>
    <dbReference type="HOGENOM" id="CLU_215067_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQ81"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="61" mass="6712" checksum="783606197237036D" modified="2012-04-18" version="1">MDSTDDNPSANAKEPDEPTYLNRSSLLERLSSPIAATLANKNPLSKPISEQLNMRISLLDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQH3</accession>
    <name>H6QQH3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21181</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EHS62673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890219.1">
        <property type="nucleotide sequence ID" value="XM_003890170.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQH3"/>
    <dbReference type="GeneID" id="13541252"/>
    <dbReference type="KEGG" id="pgr:PGTG_21181"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21181"/>
    <dbReference type="HOGENOM" id="CLU_650758_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQH3"/>
    <dbReference type="OrthoDB" id="2499564at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="22"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="190"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="297"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="190"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="391" mass="43669" checksum="A893FD1DEC32280C" modified="2012-04-18" version="1">MTGISTRDIVDRIDRLSSLIENTNINSASHKDDEKNDVKDAQTQEEEAEPSRVFGKGCVLNDDQDDAQRIIKTIIESDETFVPKDEDKKDADGSDKKPCLIRMFKGEELDENFVYTDHDMVSIISRIVNENIDCFEIEEVQTATYERLKFFNEDSKAEAKSPGWEEIVHKTPIQELFSMIEFLREQARDTSELEDLPKESPSLDKNLLPEEPSQLTKTEQADSSFLDKEKSSGSLEATADLPSDNLAQKSGDPQLIEYSIEIKDEGVASYAGKRPPGTIAPMEGGGLNGLIEVLQRFSDGPDSTEPKKLPPSRPKVAPLDLSAEVKKKPKTVETPTSALPLPLSSRLNAALLSPQSYAKFRDAALPLEELYRRNGKDREKLERFLAAHAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQQ8</accession>
    <name>H6QQQ8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21238</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EHS62776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890193.1">
        <property type="nucleotide sequence ID" value="XM_003890144.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQQ8"/>
    <dbReference type="GeneID" id="13541314"/>
    <dbReference type="KEGG" id="pgr:PGTG_21238"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21238"/>
    <dbReference type="HOGENOM" id="CLU_1042582_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQQ8"/>
    <dbReference type="OrthoDB" id="2507496at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS62776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="267" mass="29977" checksum="8C3E90043CE3D9AB" modified="2012-04-18" version="1">MAGNAYERASGQTSDAAPPKAQLRAIYLDYLVYYRPVASQLGKAGKASGSKEWDRVAPPSKSETVWKVDLSYYAWADCQQEIIRLCAPTRKHIGDHLRRLQEEGLLKWLCILHQDPQFGQNKNYYVTNDADFAPFVQAVGNNSSNKAMIKILMDDPNKSAKDSDEAKKVDDSLAMNYADEDTRLALQRVHTRLAVNPKSDVGAQERMRLVADITHHIRTKYGCNTESMRVQDPEDPKRSIRVTTNALRAWSRAMLHGAKGVDLDTAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQT4</accession>
    <name>H6QQT4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EHS62832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890151.1">
        <property type="nucleotide sequence ID" value="XM_003890102.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQT4"/>
    <dbReference type="GeneID" id="13541421"/>
    <dbReference type="KEGG" id="pgr:PGTG_21153"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21153"/>
    <dbReference type="HOGENOM" id="CLU_706237_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQT4"/>
    <dbReference type="OrthoDB" id="2511305at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="236"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="339"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="254"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="310"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="357"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="391" mass="43776" checksum="0AE4EB952E5C4F71" modified="2012-04-18" version="1">MPRRPSFISQTQPRCLASSQNACFHWPPVVEAPQVNAFGKRSYGQVAARLTNPSTSCSSNVRKVLNQKGKGVNSGSTRQTTSTRGYESIVAQSAWPTATRCRPNPSSDRGRGPGQSTWRVCRSVLRRRTSFNPGNTASSQVIPPETDHDMDENQYLDTSVEESDTPADSTIHREQVLTGGRRRLQNWVTISDDEEDEDQYLDEQSCCLPKEPIHEHPLIDTDIDELDEDQQSDCLSDDLEGEHISPEIDDEMADDRSSDDSEGKHISPPTDDEMDEDQHSNCSSDDSEGEHISPQTDDEMDEDRQSVMSVEDHLSEGGTRHAESKAHYTHELQRSLWTRSQHLNGRVRPQNHFCSPESKHDSDAERASSGKAQGRDATAQCLSEEDGRRRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRB3</accession>
    <name>H6QRB3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytochrome P450</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_21434</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="9" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cytochrome P450 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EHS63104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889965.1">
        <property type="nucleotide sequence ID" value="XM_003889916.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRB3"/>
    <dbReference type="STRING" id="418459.H6QRB3"/>
    <dbReference type="GeneID" id="13540553"/>
    <dbReference type="KEGG" id="pgr:PGTG_21434"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21434"/>
    <dbReference type="eggNOG" id="KOG0157">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_378611_0_0_1"/>
    <dbReference type="InParanoid" id="H6QRB3"/>
    <dbReference type="OrthoDB" id="1055148at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0020037">
        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004497">
        <property type="term" value="F:monooxygenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.630.10">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050529">
        <property type="entry name" value="CYP450_sterol_14alpha_dmase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001128">
        <property type="entry name" value="Cyt_P450"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002403">
        <property type="entry name" value="Cyt_P450_E_grp-IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036396">
        <property type="entry name" value="Cyt_P450_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304:SF2">
        <property type="entry name" value="24-HYDROXYCHOLESTEROL 7-ALPHA-HYDROXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24304">
        <property type="entry name" value="CYTOCHROME P450 FAMILY 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00067">
        <property type="entry name" value="p450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00465">
        <property type="entry name" value="EP450IV"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48264">
        <property type="entry name" value="Cytochrome P450"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0349">Heme</keyword>
    <keyword evidence="5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="4 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="300"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="6">
        <location>
            <position position="549"/>
        </location>
        <ligand>
            <name>heme</name>
            <dbReference type="ChEBI" id="CHEBI:30413"/>
        </ligand>
        <ligandPart>
            <name>Fe</name>
            <dbReference type="ChEBI" id="CHEBI:18248"/>
        </ligandPart>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR602403-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EHS63104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="607" mass="68811" checksum="2A3E445AC66DD21D" modified="2012-04-18" version="1">MSSSSESYLESDPTVSSKTFSLTLRPQLFFAKAQHAVAKKSQQRALSCFTGSWDITSRFKTEHNSSTNFWKYSPRIWTWPWIGSLCSYTRDPLGFIQTQHRSQGDVFTTTILGYNCTFIQSNEYIQSFATATRKVLDVTAAYKLIAAPLIGEEAFFGQTKYMHQVVLSKSRTELLHQRLWELAEELLEIKWAQCFSSGDQTEVRNWQKIDLGQMLDYVIFGLDVFFLIGEEMVNVNLDRISHLFRVMDSDLSLLGILLPMIRGGRCPRKEAKNELLGFLKRDVQRRIERHVQHAVNSAFGSSQSDCAETWPDGPEEDSQELPDILLRSELKELDEAILFDYHRGESSAIKLINEKVEFIAIFIYGFVWAAQTNSAAATIGLVHDILLHDQSQALSDEHSPCFSHAEQIRRECASAVVKDTPSPAPFLEDMPHLAHCLNETLRLRATGAWVRLADKSFHLSPQGNQTSGVICPPGFIVLSPMPVSLNPSTYPNPSRWDPYRYERAPFISSPSGSNKDAARHIPIDKQYLVPPTTPSSPYFASWGLGQGHCPGKHLAYKMISMTVARFFDKFEVRPTKEYFENANFEDVAVAGVQRLKNEFTVLIRKRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRJ0</accession>
    <name>H6QRJ0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21514</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EHS63298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889871.1">
        <property type="nucleotide sequence ID" value="XM_003889822.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRJ0"/>
    <dbReference type="GeneID" id="13540699"/>
    <dbReference type="KEGG" id="pgr:PGTG_21514"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21514"/>
    <dbReference type="HOGENOM" id="CLU_2159660_0_0_1"/>
    <dbReference type="InParanoid" id="H6QRJ0"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="50"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="111" mass="12509" checksum="FF07748CB80C1F74" modified="2012-04-18" version="1">MTEDKKTQESVGAARANVHETLHCIGYVMCMTMYPVDWRSRLDMMHVCDGAQSSGEGAAPHGHDHEHAQHRRSQDVPPHFWILNIHLQTPKGSLQRRPDPAPLYIVSDLCA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSE8</accession>
    <name>H6QSE8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21764</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EHS63678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003889517.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSE8"/>
    <dbReference type="GeneID" id="13542729"/>
    <dbReference type="KEGG" id="pgr:PGTG_21764"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21764"/>
    <dbReference type="HOGENOM" id="CLU_2905213_0_0_1"/>
    <dbReference type="InParanoid" id="H6QSE8"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="62" mass="7271" checksum="C66E64A219D10733" modified="2012-04-18" version="1">MVMGTGRWRKVMPQQDRQSISLQLTAIRTRMKVTGRLPDPNMRMRSRSRVRRPISIRAGFSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSG7</accession>
    <name>H6QSG7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21783</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178294">
        <property type="protein sequence ID" value="EHS63704.1"/>
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    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003889477.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSG7"/>
    <dbReference type="GeneID" id="13542794"/>
    <dbReference type="KEGG" id="pgr:PGTG_21783"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21783"/>
    <dbReference type="HOGENOM" id="CLU_3143672_0_0_1"/>
    <dbReference type="InParanoid" id="H6QSG7"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="49" mass="5612" checksum="68CCCA7898742DC1" modified="2012-04-18" version="1">MASFPHNLRTTGTGKFPQWVPPLKRMQALLTGWDVRFQQNACELIPKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSK4</accession>
    <name>H6QSK4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21847</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178295">
        <property type="protein sequence ID" value="EHS63755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889514.1">
        <property type="nucleotide sequence ID" value="XM_003889465.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSK4"/>
    <dbReference type="STRING" id="418459.H6QSK4"/>
    <dbReference type="GeneID" id="13541882"/>
    <dbReference type="KEGG" id="pgr:PGTG_21847"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21847"/>
    <dbReference type="HOGENOM" id="CLU_2886856_0_0_1"/>
    <dbReference type="InParanoid" id="H6QSK4"/>
    <dbReference type="OrthoDB" id="10371425at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="7"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="40"/>
            <end position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="63" mass="6873" checksum="B7866E6CD049E26F" modified="2012-04-18" version="1">MEWSKYLSHNQNQQNTNAFPIAQPPGKLAALNMAEKVPSGSKSLAQANSHKYDEQNAASTART</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSR6</accession>
    <name>H6QSR6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_21822</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178297">
        <property type="protein sequence ID" value="EHS63806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889463.1">
        <property type="nucleotide sequence ID" value="XM_003889414.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSR6"/>
    <dbReference type="GeneID" id="13541961"/>
    <dbReference type="KEGG" id="pgr:PGTG_21822"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21822"/>
    <dbReference type="HOGENOM" id="CLU_021865_0_0_1"/>
    <dbReference type="InParanoid" id="H6QSR6"/>
    <dbReference type="OrthoDB" id="2507703at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="253"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="477"/>
            <end position="708"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="713"/>
            <end position="732"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="422"/>
            <end position="476"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="24"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="59"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="69"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="89"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="152"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="176"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="203"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="509"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="550"/>
            <end position="560"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="610"/>
            <end position="621"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="646"/>
            <end position="660"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="665"/>
            <end position="691"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS63806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="747" mass="80922" checksum="9A03DE5BDD4EE799" modified="2012-04-18" version="1">MIKKSTPSPIIKTNKTTTTSPLRQQQLNQLNPSTPPTTTKTATTPIKNKNKNTSPINKLNSQNSTPRSAENHDRRSFQGDHHRHRYMTYSATTTPSPNSIQSNQQTNHHHHQQQQQQQQQHLTLLGAPPNAHYSPHYGQPVQRAPTSKRKPAPPLSPSYNPPSSPILDSLTPRTPSPSHSNSSYLHSPEISPSRRTHRKLIKSRSPSPSPRSGTAIVKKIGSALVAPFRNSNSRRGSDASEIGLSIQEVSRLSSFGPPVQPSGPASSAEHLPWMPSVGAAGSPTPGAQEEDQEGLTEAERIVRASRSRGTLADGTTGAFPERVLRVVNGSASVSTRASGESRTRDLSLEEESCKTTQPLNIVPSQNSRHSYLSTSTKAGCTKCVALEEQLRAEVARGRALERALDAQTLKLDQLRNWATAKVSSLDQTLQAQEKLLQQVEQRMAERHQPDIKSDYLIALQKDYVELNARLALLEAKEHEHDEDDPHDSSPLSPPSHYGRSTVPAYVSKLLEDPREVRPLHTRAYSTPPLATGKARPAPSTSPFSRPSWKPASPATLASKLAPPPPSTPRSPHNPRSAPVTPSSASGRPRYTSALGVKAPSPLGFAYQTRKIIDKDGDELPKRASPTVPIRSPSPPAPSSGSARISNPVSPRISSTTSNGPKHSYARPTLASSSRLSALHPSASPSSSTLLANNRKPDHARSARVSVINPPILRPAAHLGSPRHTVPSTPFNKNKAVGDLIRLFDQPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>H6QT08</accession>
    <name>H6QT08_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_21913</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="11" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178303">
        <property type="protein sequence ID" value="EHS63959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889356.1">
        <property type="nucleotide sequence ID" value="XM_003889307.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QT08"/>
    <dbReference type="GeneID" id="13541858"/>
    <dbReference type="KEGG" id="pgr:PGTG_21913"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21913"/>
    <dbReference type="eggNOG" id="KOG0475">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003181_2_0_1"/>
    <dbReference type="InParanoid" id="H6QT08"/>
    <dbReference type="OrthoDB" id="431497at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03684">
        <property type="entry name" value="ClC_3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3080.10:FF:000013">
        <property type="entry name" value="Voltage-gated chloride channel (ClcA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45711">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45711:SF6">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0868">Chloride</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="386"/>
            <end position="407"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="495"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="525"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="553"/>
            <end position="576"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="627"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="667"/>
            <end position="684"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="731"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="758"/>
            <end position="777"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="789"/>
            <end position="811"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="817"/>
            <end position="843"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="96"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="418"/>
            <end position="441"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="49"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="61"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="129"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="418"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EHS63959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1063" mass="117434" checksum="BD8CC7A80AE44681" modified="2012-04-18" version="1">MSSPQENDYQSNQYGSFSDSPRPSARPRNHRYNSSNVSFLEIPSSLGSDKLKNRQPDEHSSLIQSSSGDSRSISKNGSLLPCSYYTAADQELEPGSADDLNRHLNASPANHRLSQGRLMGSLKMTDWMSTHSPSVPSTPHAGHSRRPSYLSGNSLVTETERRSKARRWKKNYDDEFQEELVRQSGNGIRVWYESYCTIDWLHELIKESIRRKKLAQLPGWLGTFSRTWDKSQAWVLVTLVGICTAFVADQIVSTEMWLFELKEGYCANGWRTPKRFCCDSPASYTRSSTLGSIGQFISSVFDRSNLFPFLNPPSPQSPHSTVAHPFDFNATRRDYRSPAFSSKSFISQWSFKHPSSLDSPSESCPDWHPWTDLLSPHQPQSIWIEYLVYIFFSLSFAGLSSLLTVYLSRSTQIHTSTYAQSDHNDSSSTIHNNLDDPSPVPDASCSSYFDTLPTNRKSSRNQPSSTKVPPKISYFAAGSGIPEVKCILSGFVIRGYLGLSTMLTKAVGLALSVGSGLTLGKEGPLVHIACCIGNIFTRLFPKFDRNEGKRREMLSAACAAGVAVAFGAPIGGVLFSLEEVSYFFPPRVMWRSCWCAIVGAATLRVLDPFKTGKTVLFEVTYDQQWHFIELSGFILLGLVSGVLGAWLSKLNVWWTKTFRKLPCIDRHPVLEVLLVAFVTCLLAFSNRFMKLAGTELVYEMLAECPIIDPSDPTGSSISGACISDPKDTAQLILNIGIAVVLKFLITVVTFGIKCPAGLFVPSLCIGAMMGRILGYLVEYAYHSHPELSVFQISCIIPGVWAMVGAAAMLAGVTRTTLSLAVIMVELTGSLVYILPISMSVLVAKTLADTIEHRSIYDLCMNLSELPYLDAKSEYLHYAKPEDIMDRNAEVIILNGELRASDLRQSIKNMLEAPQLGSGFPLLETSENGDTRISGYVGLVELEHCLSTIQGDPICTFDGADPDVAPNGGLSPNYELPVDFGYLVDHAPVTVSVQTPMELIHEIFVRLGVRYLVVQNHNGLYLGIIEKNRHHTTIPTPNGKEDAHFEALIMGSNSCLSLSSLLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTN2</accession>
    <name>H6QTN2_PUCGT</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22164</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178318">
        <property type="protein sequence ID" value="EHS64247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003889077.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTN2"/>
    <dbReference type="GeneID" id="13541331"/>
    <dbReference type="KEGG" id="pgr:PGTG_22164"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22164"/>
    <dbReference type="HOGENOM" id="CLU_1982663_0_0_1"/>
    <dbReference type="InParanoid" id="H6QTN2"/>
    <dbReference type="OrthoDB" id="2512192at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="103"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="126" mass="13833" checksum="94A58E29A5B77A50" modified="2012-04-18" version="1">MGIESRSLPNSTGICAHDYILEDCSAILQFLNLEFVIGVKIDCALMQSEVSKEAGSKSFLMTLRSKLASMGYGPWDLAIRKNASSLPSTTRSSSFQSDLIHALKKRQRHRELQAPGNPVGVKQNAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUA6</accession>
    <name>H6QUA6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial carrier protein ymc2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22361</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
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        </location>
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        <location>
            <begin position="20"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="181" mass="19559" checksum="B03324E8D0A8095B" modified="2012-04-18" version="1">MKSLAVLFFVSTTVALVHGATTTNYDSIPRPQRGQDYIDGTYEANARVMPEMCPSSGACSMMDSGWPSKGFSCPQKADSYGVPDYSFLTAQETAMVYGGGDEISAKEWPKSCALPRKGSKMMRYSNGAWSAIYSITPTCNCPQGVEPKCTRANGLSKETCNIAVLKFCEVRSGSQVCAPKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUQ2</accession>
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        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22483</name>
    </gene>
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
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            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178348">
        <property type="protein sequence ID" value="EHS64764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    <dbReference type="STRING" id="418459.H6QUQ2"/>
    <dbReference type="GeneID" id="13541640"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22483"/>
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    <dbReference type="OrthoDB" id="5519740at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
    <dbReference type="InterPro" id="IPR051807">
        <property type="entry name" value="Sec-metab_biosynth-assoc"/>
    </dbReference>
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        <property type="entry name" value="PROTEIN YCII"/>
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    </dbReference>
    <dbReference type="PANTHER" id="PTHR33606:SF3">
        <property type="entry name" value="PROTEIN YCII"/>
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    </dbReference>
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            <begin position="37"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="145" mass="15924" checksum="55BEDE33BFF514F0" modified="2012-04-18" version="1">MPSVVISSSIRFLRLLQLQSGFNLPSHHRSFAKNLARTAPKKEIDPDVKWSSPLPKQTPSDAPNDAKEAADAKVFTYLVVAPDLGNSKRAILRAEHFKMAQEGLKTCRIVNAGAMLESDASESNNFDPKMIDDEDCGSNLTKINV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
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            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
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    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178396">
        <property type="protein sequence ID" value="EHS62848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003888485.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QVB9"/>
    <dbReference type="GeneID" id="13540811"/>
    <dbReference type="KEGG" id="pgr:PGTG_22640"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22640"/>
    <dbReference type="HOGENOM" id="CLU_2543684_0_0_1"/>
    <dbReference type="InParanoid" id="H6QVB9"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="83" mass="8659" checksum="25EAF007526A2484" modified="2012-04-18" version="1">MGTGDTEAGAASAGTTEGGEGAVEDPSQRVDRASRALALKDWRWASWGGSTRSGGYLGGGIEYRNPSGSRTSRRKHAEPCCSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQ04</accession>
    <name>E3JQ04_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00043</name>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74087.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003307045.2"/>
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    <dbReference type="AlphaFoldDB" id="E3JQ04"/>
    <dbReference type="STRING" id="418459.E3JQ04"/>
    <dbReference type="GeneID" id="10546807"/>
    <dbReference type="KEGG" id="pgr:PGTG_00043"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00043"/>
    <dbReference type="eggNOG" id="KOG1216">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_413395_0_0_1"/>
    <dbReference type="InParanoid" id="E3JQ04"/>
    <dbReference type="OrthoDB" id="3366093at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005034">
        <property type="term" value="F:osmosensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007232">
        <property type="term" value="P:osmosensory signaling pathway via Sho1 osmosensor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039295">
        <property type="entry name" value="MSB2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35778">
        <property type="entry name" value="SIGNALING MUCIN HKR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35778:SF1">
        <property type="entry name" value="SIGNALING MUCIN HKR1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="7"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="88"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="124"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="160"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="235"/>
            <end position="261"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="283"/>
            <end position="304"/>
        </location>
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    <feature type="region of interest" description="Disordered" evidence="1">
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            <begin position="459"/>
            <end position="487"/>
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    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="505"/>
            <end position="540"/>
        </location>
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    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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            <begin position="88"/>
            <end position="107"/>
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
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    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="235"/>
            <end position="245"/>
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    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="246"/>
            <end position="260"/>
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        <location>
            <begin position="283"/>
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        <location>
            <begin position="459"/>
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        <location>
            <begin position="522"/>
            <end position="540"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP74087.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="664" mass="70785" checksum="414976F76E0F8D93" modified="2012-04-18" version="2" fragment="single">MMMIIIITISSNPTQPNPSKPSPPNTDENNLGSFLAKDNLTSASIPFKFNTTATTPQPTKNSGSEQTENNLASFLSKDNITSASIPFKFNSTTTQPTKNSSPNTDDNLASFLSKDNITSASIPFKFDNTKTQPSKNSSPSTDDNLASFLSKDNITSASIPFKFNNTSTQPTKNSSPNTDDNLASFLSKDNITSASIPFKFNNTSTQPTKNASPNTDDNLASFLSKDNITSASIPFKFNNTNVGTGQQTKKNSSSSSNTDDNLASFLSKDNLTSASIPFKFNSTNVGTSQQTKNPNSNTDDNLASFLSKDNLTSSSTPFKFNTTAAALTSSSKFNSTTENNDQPSLINAMNATMTSSPFKYNTTNENNLDQANASLLSAPFKFNQTNSTSAHSTNSESEPSLAAIGQDKAANTTLVSNTFQLNSTTGAGLQSNNVTAIDKDNAVLTSSPFKYNSTNLNSSDSNGLLSSNNSNNNATNSTPVYTPYHSDPKPAPAFVPVTSLFVNPRPADPVGPSTAPPSVETSLPSAGAPPKKSSPTPFPSSWPRYIVPTNAPTSAPANSTLISILFTDALNWPWLVTNSNASSQVLVFMPELIASSLQIPEDQVVTQSLQAYQPVNFNPSNEASMLTLWLGYIPSQYVGQLQAMIRAPQSRFYSSTNSGFESLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQS4</accession>
    <name>E3JQS4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Pre-mRNA-processing-splicing factor 8</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_00662</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003307664.2"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00662"/>
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    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000380_3_0_1"/>
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    <dbReference type="OrthoDB" id="1931567at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
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    <dbReference type="GO" id="GO:0097157">
        <property type="term" value="F:pre-mRNA intronic binding"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="project" value="UniProtKB-ARBA"/>
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        <property type="entry name" value="RNase_H_like_Prp8_IV"/>
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        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
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        <property type="entry name" value="Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding domain"/>
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        <property type="entry name" value="JAMM/MPN+_dom"/>
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        <property type="entry name" value="PRP8_domainIV"/>
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        <property type="entry name" value="Prp8_domainIV_fingers"/>
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        <property type="entry name" value="Prp8_U5-snRNA-bd_sf"/>
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        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
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    <dbReference type="PANTHER" id="PTHR11140">
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    </dbReference>
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    <keyword evidence="5" id="KW-0508">mRNA splicing</keyword>
    <keyword evidence="6" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0747">Spliceosome</keyword>
    <feature type="domain" description="MPN" evidence="8">
        <location>
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            <end position="2282"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
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            <begin position="10"/>
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            <begin position="32"/>
            <end position="46"/>
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        <source>
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    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022728"/>
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    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
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    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50249"/>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP74706.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="2384" mass="275907" checksum="E9962159C7F43D20" modified="2012-04-18" version="2">MAQPPPSVPPNNNNLNHPLPVPPASSSSSFVPTPPHLAPHGLPAPPHLAGMPPVSYQEPAQMTQEELDLKAKKWTQFQQRKYGDKQVKTSFVDTGKQEMPPEHVRKIIKDHGDLSNRKFRTDKRVHLGALKYVPHAVMKLLENMPMPWEQVREVPVIYHITGAITFVNEVPKVIPPVYHAQWASMWLSMRREKRDRRHFKRMRFPPFDDEEPPLDYGDNILDTEPLEAIQMDLDEEEDAPVFDWFYDHKPLVKKYKGVQYVNGSSYKSWQLDLGMMSTLYRIGRNLLSDFIDNNYFYLFEPKAFFTAKAMNMAIPGGARFEPLHRDAESYDDDWNEFNDINKVIIRQQIRTEYRVAFPHLYNSRPRSVHIPKYHEVKNLFIRVEDPDLPTFYFDPIIHPISSRAVAPQNQGVSHEEEIFGDADEDDFQLPDDLEPFLADKPIADDNTTSAIAMWWAPYPYNHRSGKTIRTLDVPLVKNWYMEHCPPGMPVKVRVSYQKLLKNYVLNELHKRKPKPQSKKYLFRQLKNTKFFQTTRLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNMNLKPVKTLTTKERKKSRFGNAFHLCREILRLTKLIVDCHVQYRLGNVDAFQLADGLQFTFAHVGQLTGMYRYKYKLMKQIRQCKDLKHLIYYRFNTGAVGKGPGVGFWAPGWRVWLFFMRGIVPLLERWLGNLLARQFEGRNSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGLKQNKSKTILQHLSEAWRCWKANIPWKVPGMPTAVENIILRYVKSKADWWTSVAHYNRERIKRGATVDKTVSKKNLGRLTRLYLKAEQERQNSYLKDGPYVTAEEAVAIYSATVHWLESRKFAPIPFPPLNYKHDTKLLVLALEKLKEAYSVQGRLNQNQREELALIEQAYDNPHETLSRIKRLLLTQRAFKEAGLEFFDTYDKLIPVYDIEPIEKITDAYLDQFLYFEADKRGLFPAWIKPADTEPPPLLVYKWCQGINNLHEVWETADGECNVMLETQLSKPYEKMDLTLLNRLLRLIMDHNLADYITAKNNIVLTYKDMSHVNSYGLIRGLQFSSFVFQFYGLVLDLLIMGLQRASEMAGPPSMPNNFLQYRDTSTEVRHPIRLYARYVDKIHILFRFTAEESRDLIQRFLSANPDPNNENIIGYNSKRCWPRDCRMRLIKHDVNLGRAVFWDIKNRLPRSLTTIEWVDTNASVYSKDNPQLLFSMSNFEVRILPKIRNVGEAFTLKDGVWNLVNEATKERTAQAYLRVSDYGIDQFHNRIRQVLMASGSTTFSKIVNKWNTAIIGLMTYYREAVIHTNEMLDLLVKSENKIQTRVKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHILIPASDLRWSKQTDTGITHFRSGMSHEEDQLIPNLYRYIQPWSSEFEDSARVWSEYAMKRKEANSQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDWKQYQLLKHNPFWWTNQRHDGKLWQLNNYRVDVIAALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKFKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESVVMDLCQVFDQELEALQIETVQKETIHPRKSYKMNSSCADILLFSAYKWNMTRPSLLTDSKDVLDGTTSQKYWLDVQCRWGDFDSHDIERYTRSKFLDYSQDNTSIYPSPTGLMIGIDLAYNLHSAYGHWFPGAKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLNSQNYSELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQVIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQSAMKLEKFGDLILRATQPQMILANLFDDWLKSISSYTAFSRLILLLRALHVNQEKSKIILRPNKSTITQPHHVWPTLSDEEWMRVELALRDLILADYAKRNAVNTASLTSSEIRDIILGMEIQAPSIQRQQMAEIEKTAEQANQVTATQTQTTNVHGDLIQVVTTTQYETQTFSSKTDWRIRAISSTNLPLRLQHCYVQNDDVSDSLPTFVLAKNIMKSFVCAADLRTPIAAILYGQAAPDNNRVIEVRAVAVIPQRASQRSIELPNALPKHPLLDDMKIVGVIHTQAQESQTLSPNDAILFSKLMAQHSQLDGTSIMLTVAFTPGSLSLSAYVLTPKGFEWGRNADPNAPNGYNPASMSERVQLLLSDRILGSTFVPQDGIWNYSVGLGPRFQTTMEYTMTLAGQPIPFWDPSHRPNHFSAFQNIAVEEEPTIDTADPFA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3JTZ2</accession>
    <name>E3JTZ2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_00844</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75513.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319932.2">
        <property type="nucleotide sequence ID" value="XM_003319884.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JTZ2"/>
    <dbReference type="GeneID" id="10543229"/>
    <dbReference type="KEGG" id="pgr:PGTG_00844"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00844"/>
    <dbReference type="HOGENOM" id="CLU_2085958_0_0_1"/>
    <dbReference type="InParanoid" id="E3JTZ2"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP75513.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="117" mass="12755" checksum="1DF43FFA29F0295C" modified="2012-04-18" version="2">MPEKGGLGVFAAQSFKRGDYVDVVPNGTSCPLKFQVSEAASRGNDSASARIAIVNPLIAAHVGYSAVEGTVSYHKRKNLTEPFPFLGLAVEDGWKTQSPHEIILMRIDDGFDDWEFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUS3</accession>
    <name>E3JUS3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_01129</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75798.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320217.2">
        <property type="nucleotide sequence ID" value="XM_003320169.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JUS3"/>
    <dbReference type="GeneID" id="10543792"/>
    <dbReference type="KEGG" id="pgr:PGTG_01129"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01129"/>
    <dbReference type="HOGENOM" id="CLU_1750604_0_0_1"/>
    <dbReference type="InParanoid" id="E3JUS3"/>
    <dbReference type="OrthoDB" id="2497480at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP75798.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="149" mass="17592" checksum="3025A99927BD82F0" modified="2012-04-18" version="2">MTMNNRHIIRLNNCVTIAVWLTNLNPSRFYPPREFSMVIITVPSRFGLVAYRVTSQRSRLRSLHLGTFRISWGDPEVPENSRVISDHFLQRVEAFTRRMWGNQAAHERFESSCARWLLGLLRRELGHDFFDHHRLHLVMEKVVALLHSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUV4</accession>
    <name>E3JUV4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Medium-chain specific acyl-CoA dehydrogenase, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_01160</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the acyl-CoA dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75829.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320248.2">
        <property type="nucleotide sequence ID" value="XM_003320200.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JUV4"/>
    <dbReference type="STRING" id="418459.E3JUV4"/>
    <dbReference type="GeneID" id="10532744"/>
    <dbReference type="KEGG" id="pgr:PGTG_01160"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01160"/>
    <dbReference type="eggNOG" id="KOG0140">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_018204_0_2_1"/>
    <dbReference type="InParanoid" id="E3JUV4"/>
    <dbReference type="OrthoDB" id="9988775at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070991">
        <property type="term" value="F:medium-chain fatty acyl-CoA dehydrogenase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051793">
        <property type="term" value="P:medium-chain fatty acid catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.110.10:FF:000054">
        <property type="entry name" value="Acyl-CoA dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.540.10:FF:000010">
        <property type="entry name" value="Medium-chain specific acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.140.10:FF:000011">
        <property type="entry name" value="Medium-chain specific acyl-CoA dehydrogenase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.540.10">
        <property type="entry name" value="Acyl-CoA dehydrogenase/oxidase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.110.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.140.10">
        <property type="entry name" value="Butyryl-CoA Dehydrogenase, subunit A, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050741">
        <property type="entry name" value="Acyl-CoA_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006089">
        <property type="entry name" value="Acyl-CoA_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006091">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046373">
        <property type="entry name" value="Acyl-CoA_Oxase/DH_mid-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036250">
        <property type="entry name" value="AcylCo_DH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009075">
        <property type="entry name" value="AcylCo_DH/oxidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013786">
        <property type="entry name" value="AcylCoA_DH/ox_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037069">
        <property type="entry name" value="AcylCoA_DH/ox_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009100">
        <property type="entry name" value="AcylCoA_DH/oxidase_NM_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083:SF2">
        <property type="entry name" value="MEDIUM-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48083">
        <property type="entry name" value="MEDIUM-CHAIN SPECIFIC ACYL-COA DEHYDROGENASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00441">
        <property type="entry name" value="Acyl-CoA_dh_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02770">
        <property type="entry name" value="Acyl-CoA_dh_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02771">
        <property type="entry name" value="Acyl-CoA_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47203">
        <property type="entry name" value="Acyl-CoA dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56645">
        <property type="entry name" value="Acyl-CoA dehydrogenase NM domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00072">
        <property type="entry name" value="ACYL_COA_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00073">
        <property type="entry name" value="ACYL_COA_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0274">FAD</keyword>
    <keyword evidence="4 7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase N-terminal" evidence="11">
        <location>
            <begin position="55"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA oxidase/dehydrogenase middle" evidence="10">
        <location>
            <begin position="169"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="Acyl-CoA dehydrogenase/oxidase C-terminal" evidence="9">
        <location>
            <begin position="277"/>
            <end position="424"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP75829.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="431" mass="46621" checksum="72D379D6C738E88E" modified="2012-04-18" version="2">MFTGRARLSLPAASSRPRSRLPLTSSGPLKTFRPYSSSSSDRDLLAGPVNFALSADQQAYQDLARRFTVDHVIPVAAEYDRTMEYPWPVLKEAWKAGLLNTHVPEEYGGIGIGLLESALISEEIAYGCTGIQTAFEASGLAQAPLIVAGSDELKRKYLSMLIEEPLVAAYCVTEPGAGSDVAGIKTQAVKTGNDFVLNGSKMWITNGGKASWYFVLAKTDPEAPVHKSMSGFIVDANSPGVLVGKKEINMGQRCSDTRQITFEDVVVPSSNLVGAMGEGFKVAMAAFDITRPLVASGAVGLAQRALSEAVKYAQTRKTMGKPIIEHQSVSHMLAEMTIGVEAARAMVWKSTFIKDTGHRNTYYASIAKCLSSEVAVRNAGMAVQIFGGAGFNTEYPVEKLYRDSKIFTLYEGTSQIQRLIISKFLPTMYPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3JV53</accession>
    <name>E3JV53_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">SGNH hydrolase-type esterase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_01259</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75928.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320347.2">
        <property type="nucleotide sequence ID" value="XM_003320299.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JV53"/>
    <dbReference type="FunCoup" id="E3JV53">
        <property type="interactions" value="88"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JV53"/>
    <dbReference type="GeneID" id="10533006"/>
    <dbReference type="KEGG" id="pgr:PGTG_01259"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01259"/>
    <dbReference type="eggNOG" id="KOG3035">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_051989_0_2_1"/>
    <dbReference type="InParanoid" id="E3JV53"/>
    <dbReference type="OrthoDB" id="671439at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01838">
        <property type="entry name" value="Isoamyl_acetate_hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1110:FF:000092">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1110">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045136">
        <property type="entry name" value="Iah1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013830">
        <property type="entry name" value="SGNH_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036514">
        <property type="entry name" value="SGNH_hydro_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14209">
        <property type="entry name" value="ISOAMYL ACETATE-HYDROLYZING ESTERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14209:SF19">
        <property type="entry name" value="ISOAMYL ACETATE-HYDROLYZING ESTERASE 1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13472">
        <property type="entry name" value="Lipase_GDSL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52266">
        <property type="entry name" value="SGNH hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SGNH hydrolase-type esterase" evidence="1">
        <location>
            <begin position="27"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75928.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="277" mass="31134" checksum="1B69B0EA49319C45" modified="2012-04-18" version="2">MVTVRPTQSEMSTTKCEPFSMDELVMFGDSITQFAWQAGGTGSALADYYQRRLDVVNRGFSGYNTTWGLHVAKKIFPAKSPESYSFPRKRIVTIWFGANDAVIPPKPQTVTPEQFVENMNKLIEMVQAHASPTNSQQDQSQLLIVLITPPPISVALRAADLASRFPDWRPENMDRDPVRTRLFAELVCQVAQQKGLPVIDTWTAITKAADESQGGLSTYLVDGLHLTPAGYEIVTQEFKSILTRHYPSFLPESLPHDFPWWNDIDAQHPENSFPECC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3JVJ8</accession>
    <name>E3JVJ8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02514</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320492.1">
        <property type="nucleotide sequence ID" value="XM_003320444.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JVJ8"/>
    <dbReference type="GeneID" id="10533475"/>
    <dbReference type="KEGG" id="pgr:PGTG_02514"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02514"/>
    <dbReference type="eggNOG" id="ENOG502R2FC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_548757_0_0_1"/>
    <dbReference type="InParanoid" id="E3JVJ8"/>
    <dbReference type="OMA" id="PQINGAN"/>
    <dbReference type="OrthoDB" id="2507662at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="37"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="105"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="381"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="455"/>
            <end position="497"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="47"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="82"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="160"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="388"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="455"/>
            <end position="469"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="497" mass="54350" checksum="D4FEE7289908374D" modified="2011-01-11" version="1">MTEEQLTNEQQQQHKNNNNNNHHHSRLTRFLSKFTINPTNHSKRKSSLPNLNLNNSNNNNSNNNRQTEIESNTTKQRSLSHHPQDTQKIKLDSISLQPLLPLPTIIDRHSNSSSSAASTNQENHHHSEADPEDGADPNASIRPITPSSKANSFLPSPSETPTHQHQQQQPALNSPTQSRHSYRPTKRSSTISNSSSEPILDDCKSTFGNSSLASTKPTTVISLENGPANRIAQPHPSPITTHHYHHNHHNHNPNHNHHNNITNHSNMAQPAQNNLRNPIRAAIINHQRVQSASSSIINQPIQPTTPSLTLHSSPPISPQNHFSIINVPRHSLPHPSQNPHPAALADNASILTLASSGFSPSLHHQQNFPSDEDASVRALAPSRRESIESLSSRWSNVKSTRTGGSIRTTGTGFYTGIGPSSSVQNDSVLTPVDQHPGGDSPALFQDASELNIPSPLLLPETTTTTPLVEPGHHTLDLNHSSPVAEKTQLAQEIVPPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3JVK9</accession>
    <name>E3JVK9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF6818 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_02525</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320503.1">
        <property type="nucleotide sequence ID" value="XM_003320455.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JVK9"/>
    <dbReference type="GeneID" id="10533488"/>
    <dbReference type="KEGG" id="pgr:PGTG_02525"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02525"/>
    <dbReference type="HOGENOM" id="CLU_622779_0_0_1"/>
    <dbReference type="InParanoid" id="E3JVK9"/>
    <dbReference type="OrthoDB" id="3055425at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049203">
        <property type="entry name" value="DUF6818"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34409:SF1">
        <property type="entry name" value="MYB-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34409">
        <property type="entry name" value="SET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20681">
        <property type="entry name" value="DUF6818"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6818" evidence="3">
        <location>
            <begin position="63"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="134"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="368"/>
            <end position="440"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="272"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="156"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="2">
        <location>
            <begin position="188"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="201"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="369"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="400"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="418"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF20681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP76084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="440" mass="49081" checksum="0F79D9E7CC2EAB72" modified="2011-01-11" version="1">MVSNSQPNPAQTATTEARPTQPPLSQSANSTAPQRRQGRMPGSQGYNTDDCVGLIKCVGRVMPMGPQQWSSVQEAYHKYADKNNRSHREIDSLKHKFRAIANSRKPTGDPNCPAYIRDAKRIQIEIDQKTRKYVAEDQSDDEQNGDGAHMEDSQSLDRNASPGSQVSNSSETSPNHQESEPNIPTNDDINDQMELDDQSSSHHPPTNESQASKANLCRRGASSSSQRIHHTYSPMNSSTRRSQRGRRGLDQCLETYFDPEARDNRERDQGMASFYAARLKEANTKIEALSRENQDLSTKVDNEKRDLKAEVDRLRAESTKKSMDIRDLRGKLDLLQLKLDLYKRGQFQPQPRPMYNYQGPYMTAGHAMQTPSQTAYAPAAQSNFPTQSAPSVPPPVPINSAPSVPPVQSSYQPPPESFSSMGETNFGQQNEHLGTHPSEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>E3JVX4</accession>
    <name>E3JVX4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="7">CAMK/CAMK1 protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_02640</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the protein kinase superfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320618.1">
        <property type="nucleotide sequence ID" value="XM_003320570.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JVX4"/>
    <dbReference type="STRING" id="418459.E3JVX4"/>
    <dbReference type="GeneID" id="10533767"/>
    <dbReference type="KEGG" id="pgr:PGTG_02640"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02640"/>
    <dbReference type="eggNOG" id="KOG0032">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="40902at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
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        <property type="evidence" value="ECO:0000318"/>
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    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
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        <property type="term" value="F:calcium/calmodulin-dependent protein kinase activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034599">
        <property type="term" value="P:cellular response to oxidative stress"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05117">
        <property type="entry name" value="STKc_CAMK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:001194">
        <property type="entry name" value="Calcium/calmodulin-dependent protein kinase type 1D, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24347">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="6">
        <location>
            <begin position="33"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="342"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP76199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="397" mass="44137" checksum="9DADB5ABA3999C7D" modified="2011-01-11" version="1">MPDSALASLHPKRRVLRALGLRDPNLHARKSEFKWGAHLGSGTYGEVTQCTWLTKNPPQEVAIKVVAKSSVPNLQQHISLMNRTLKLHHPHIVQMYDWFQSKEHFYMVLELATGGELFDHTITRGNFNDREACEMIHQVLDALSFIHSQGIVHRDLKPENLFVRGSAPYVPPYDLVVADFGVAVYVDRPDGSTASLTGICGSPGYTAPEVYRRLPYGKPVDIWATGVIAFILLSGRFPFAHLSGQAFLDDSDAEIKFPKRFAISPQAQNFILGCLQTEPSKRWTAEEALEHPWFDQLEEPIEEDVGERQTAASVADTDHTRCEQLPLVRADEPDDKQVESLATTAASSAERVGSALPPPSSHLSTPRSDDDPEPQLVLVHRSHTVNEDPPLVVDIVP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3JW13</accession>
    <name>E3JW13_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_02679</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76238.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320657.2">
        <property type="nucleotide sequence ID" value="XM_003320609.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JW13"/>
    <dbReference type="GeneID" id="10533968"/>
    <dbReference type="KEGG" id="pgr:PGTG_02679"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02679"/>
    <dbReference type="HOGENOM" id="CLU_1907716_0_0_1"/>
    <dbReference type="InParanoid" id="E3JW13"/>
    <dbReference type="OrthoDB" id="10391419at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173307" evidence="2">
        <location>
            <begin position="24"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="48"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="70"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP76238.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="133" mass="14714" checksum="C463285AA41F0DE9" modified="2012-04-18" version="2">MQIISPSLAVCVAVVVLFVETLAQGSGRNLSKRTHNLESTPLISTKLRSTSEIFKDESGGHRASEFGTPRPTSNQLAHTMPPNQGSWSNLDGNEHSKTYVDLQGNLYPEVKKFLRDVKNEVGSVHQSKEDYDM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWN9</accession>
    <name>E3JWN9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Translocation protein sec72</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_02905</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320883.1">
        <property type="nucleotide sequence ID" value="XM_003320835.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JWN9"/>
    <dbReference type="FunCoup" id="E3JWN9">
        <property type="interactions" value="166"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JWN9"/>
    <dbReference type="GeneID" id="10528235"/>
    <dbReference type="KEGG" id="pgr:PGTG_02905"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02905"/>
    <dbReference type="eggNOG" id="ENOG502S442">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_090376_2_0_1"/>
    <dbReference type="InParanoid" id="E3JWN9"/>
    <dbReference type="OMA" id="KMYTLAI"/>
    <dbReference type="OrthoDB" id="433738at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904:SF523">
        <property type="entry name" value="STRESS-INDUCED-PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904">
        <property type="entry name" value="TPR REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0802">TPR repeat</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="21"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP76464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="244" mass="26520" checksum="6E134FED7E5D5451" modified="2011-01-11" version="1">MPPAFSPHNPTAHLKSESHEGGGGGCCGGHGVPKVELPDPTPLDELITIHNLSVDPETHQVSLSSKSSASTQKPTEQKLLQDTLSIINDLSQYLSRAPFPHCPPPPQMVQNNPRSAQITALKEEGNAAFKANDFNKAIELYTVAANAASTRPLFEPSVWIREELAVVLCNRAAAYSSAKMWVEALCDAEVVIQLKRNWNKGHFRKVKALQGLGHIEEAKDAALLGLEFEPDNSDLQAAYQELQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3JY84</accession>
    <name>E3JY84_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_02470</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP77009.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321428.2">
        <property type="nucleotide sequence ID" value="XM_003321380.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JY84"/>
    <dbReference type="GeneID" id="10529276"/>
    <dbReference type="KEGG" id="pgr:PGTG_02470"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02470"/>
    <dbReference type="HOGENOM" id="CLU_1396973_0_0_1"/>
    <dbReference type="InParanoid" id="E3JY84"/>
    <dbReference type="OrthoDB" id="10427199at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP77009.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="195" mass="20850" checksum="2C15AB737732A116" modified="2012-04-18" version="2">MPEVNAPVIEVCQRAGDPFMRPLLILSSEPLVHWIVTSPPSCLTGVVTPGTLKLPEVIASVIEMCQRAGDPFMSSHLILVHWGSHSRYVKVAGGDYLTNRVKIRSTGVVTPGILRMPEVIIGGLENFWELVFGGSVINSSSSSMPLPLADYVSSTSWPTSSQLTSDSTSSHDGFPLGSSSELVSISGNVQFPEVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZB2</accession>
    <name>E3JZB2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MATH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03343</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321806.1">
        <property type="nucleotide sequence ID" value="XM_003321758.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZB2"/>
    <dbReference type="STRING" id="418459.E3JZB2"/>
    <dbReference type="GeneID" id="10538097"/>
    <dbReference type="KEGG" id="pgr:PGTG_03343"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03343"/>
    <dbReference type="HOGENOM" id="CLU_453516_0_0_1"/>
    <dbReference type="InParanoid" id="E3JZB2"/>
    <dbReference type="OrthoDB" id="2501322at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031625">
        <property type="term" value="F:ubiquitin protein ligase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043161">
        <property type="term" value="P:proteasome-mediated ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030162">
        <property type="term" value="P:regulation of proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.710.10">
        <property type="entry name" value="Potassium Channel Kv1.1, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011333">
        <property type="entry name" value="SKP1/BTB/POZ_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24413">
        <property type="entry name" value="SPECKLE-TYPE POZ PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="534"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="543"/>
            <end position="569"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="602" mass="67464" checksum="03D374C81C8F5D93" modified="2011-01-11" version="1">MIISPIEWREEPGVEFWTANYKFEWTFENCTRELLENSPVKLLSRPFGDNRWQISVGTIAKTPGYLGVFLGAAQDIELLTSKTWIRRCESCTATLGTPLSRGAYGMNKNISGGYTFNAGLESRRGQAKYIKLDKLFMDPAFIIEKALFVEVTLSERRRTTQPSPFGDIQRFAGRSARFSTLFDDPECSDVCFVFPKRKGKGVRTIYAQKYIIRSLPYFNTSSVPPHPSLKICDRELEDVSSPGSTFAGQLVPEGSTTASIADDSAMTDGPSVLLDGSTYDEDDSDFELDDLDDIMRGVQQAADHLTLADVGNLSDGQQFLGPTTADRPSKKRRISQLADKQNQADDDIEVDDVAPKKGIHRVTITSTPYTTYRALLLYIYSNSIAWAPLRSNWLVHKQRMEAKGRSPGRRAAFVREHAVKFSLAMEMDELQPTSPKSMFVLADMLQLPELRQEALDQFMRSLQVDNICIELASSFLGKYDRVRQLAFEFLKSNWKSVIQTHSFTLLMDEGLSNVSAAVRTDFQKLFKCLDVSPSPSSIKQQTNNHNNNNNNNGANNPNPAANPAGPAHNLHIRQNGLNPAFPQGNNPVQLIHHPNVHYPRHQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZP6</accession>
    <name>E3JZP6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HMG box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03477</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
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    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
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                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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                <person name="Martin F."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
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    </evidence>
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    <accession>E3K0G7</accession>
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        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
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                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
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                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
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                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
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        <scope>NUCLEOTIDE SEQUENCE</scope>
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    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
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                <person name="Morin E."/>
                <person name="Murat C."/>
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                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
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                <person name="Selles B."/>
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                <person name="Tuskan G.A."/>
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                <person name="Van de Peer Y."/>
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                <person name="Ellis J.G."/>
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                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
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    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="901"/>
            <end position="910"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="932"/>
            <end position="941"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1009"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1029"/>
            <end position="1044"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77792.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1204" mass="132368" checksum="F49812521E91516F" modified="2012-04-18" version="2">MNKSLQMCGRIGYGHIELELGGALRQEYTPIPQPPPNLCITLSRMENNSEQQPPTTPQWTSSAHIKPLKLPAKVLQRKSKPKPPNTTETKNSPTSNNNNPTHHSSNLPNHSHHTAQPAIRNYAYPTTTTTTTTTNHSRNTLNSRTTTTTTLPSSSKVPILSRSTSYSQYQPAPSSSSSSSSSSSIQYHYLKHRPPSPTPSSSSSTTTDLFAGPRTSIHLDPLTGELLSAAHDVSAMDALIDTLKNDPSSPRLKPHPRSSTRSNPQPHPSTNPSYSIRPSNIPRARPPPSSITTTTTTTTTNTTTGTRIGKIHSLSTNDLTDHQSSNLAIDLQQPSQFNRPDLFPHSPHFNPRESTRSAQSKLSLRCLSRSSHPSQLVQSNPSSNLTSPIQVTSPSQVHLTSAPLPTPPLSAIPSINGIIEKYSKKLHYPPVPPARSPQLKIDSIDEIVEKYNPILKRSTRQDFNQQQQQQQKQQQLTTHTQSNPKHLSPVQDKHHPTGLPITRLRSVSENSDDSHSSINSLTKEALSLQTLLSKQTERSSQPTHFINSPSGSPKLRQRDPDSISKRSFSPTQRSPIKPSTMAEDRASAKSQADDQIALYLRSTRLTTLIKLQRQQSPMTVSLADVGSPTGHPVIVFLGLGCVRYLVGLYDELAEALGLRIICIDRWGLGKTTEVPDEQRGFLEWASVVEEVADQLLLDRFSILAHSAGAPYALASSLRLADRVYGSIHLLAPWVSMTAEGGINAGAYKWLKYIPNSMIKTVQAAEWKMTGWRLGKPPSLNHPPVGFDARAPVSSNHPSQLADLDEEEPRTSLEDELESHRPSHPPPPPIRRASKKSGTSSEEVEQSYRSRISLESNDRRSIVTDSSMGRSKNSTRFNGSSSSSPLATNNHSLKANLFGKIFNSQSGPSENSSHHHSLKPADPSSPFDHHKSSTLNRVSPNTTPLPLFLNSVASPIPSYGGSCEDETDEKNGFIVNHHKDNNHLPIYSNNKRTSQKMKSCYSESKCSSRKKAEDMTEEGARRTRSQTMLQQPISRQASSSSQSQPPILPMKPEDEGAGGSQQSHHQSTSEGKSRVRRSLGISLLRASYSESLRGGTSDLITILEKNSKPWGFSYAEIDKPVKIWHGDKDERLSFKGSQWFINSIDKLHVKNQSSLVIVRDATHSLMTDVNVLYQVFESIADQWSASASSLHLSPAASVPFPTPNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3K0P8</accession>
    <name>E3K0P8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03829</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322292.1">
        <property type="nucleotide sequence ID" value="XM_003322244.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K0P8"/>
    <dbReference type="GeneID" id="10540858"/>
    <dbReference type="KEGG" id="pgr:PGTG_03829"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03829"/>
    <dbReference type="HOGENOM" id="CLU_1960674_0_0_1"/>
    <dbReference type="InParanoid" id="E3K0P8"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="55"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="128" mass="13920" checksum="168118BBAC94A06A" modified="2011-01-11" version="1">MLPSTGFSFRDPRQILSSEHAETFPAVSWQRGCSVRCLRGTFDSIDEGQLWSMGTGEMWPRASDTKANEADNPGARSDHGSGTGACMPRPDLIDHLGSRDVVGNSCAESDTYLKWLNQLMQGDGNGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>E3K0Y7</accession>
    <name>E3K0Y7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="11">CMGC/DYRK/YAK protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_03918</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP77962.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322381.2">
        <property type="nucleotide sequence ID" value="XM_003322333.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K0Y7"/>
    <dbReference type="FunCoup" id="E3K0Y7">
        <property type="interactions" value="315"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K0Y7"/>
    <dbReference type="GeneID" id="10541110"/>
    <dbReference type="KEGG" id="pgr:PGTG_03918"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03918"/>
    <dbReference type="eggNOG" id="KOG0667">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_88_2_1"/>
    <dbReference type="InParanoid" id="E3K0Y7"/>
    <dbReference type="OrthoDB" id="9332038at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004713">
        <property type="term" value="F:protein tyrosine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14212">
        <property type="entry name" value="PKc_YAK1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000380">
        <property type="entry name" value="Serine/threonine-protein kinase ppk15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050494">
        <property type="entry name" value="Ser_Thr_dual-spec_kinase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058">
        <property type="entry name" value="DUAL SPECIFICITY PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24058:SF17">
        <property type="entry name" value="HOMEODOMAIN INTERACTING PROTEIN KINASE, ISOFORM D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="215"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="581"/>
            <end position="615"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="649"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="764"/>
            <end position="794"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="47"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="672"/>
            <end position="689"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="764"/>
            <end position="780"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="781"/>
            <end position="792"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP77962.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="874" mass="97697" checksum="9B1AD67D653A7FC2" modified="2012-04-18" version="2">MNLPANNINNSNQQQQQQQQQQQHQYYTNNTNYHQNLNQSSSHSSSQAPSTEQYYGSRSASRTSSHPQHPSATQTDQIGFRRLIPGSAHLFPILHDTPVERRVNPYDGTFISPLSALTIHLADTYRLVNPAFRYELSLNPRRVLTKPSKPAGNNGLDNEDSDYILYVNDILGGSPSSTTTDPNQLQLLQQQQHQQQQQDPNHQIRSIRSSASHKYLILDVLGQGTFGQVVKCQDMKTHQVVAVKVVKNKQAYFSQSMMEVTILELLNNTWDPENKHHILRMKDTFIHHNHLCLVFELLSSNLYELIKQNSFRGLSTSLVRVFTNQLLDALTVLNEAHLIHCDLKPENILLTSLSSPEIKVIDFGSACHERQTVYTYIQSRFYRSPEVILGLPYSSAIDMWSLGCICVELFLGLPLFPGTSEYNQITRIVEMLGMPPSYMLDVGRQTTQFFDVIEDTFNGARKVYKLKSLEQYSKEHNVAEQPSKRYFQQNTLEDIISQYPVIRKGMKPADLEKEKQNRIAFIDFVKGLLNINPIERWSPQQARLHPFILGEPLREPYIPMKSGFVSNPGGDRVMELAQKGYGHSPIDPQASPQTAGGAGSNDLSKRPYGGLPAAPVRSGQKVYDAAAYSQLQAQQQVINAQAAAMRQHQVQAAQQSQNPYALPQDNRSNLTQLPPQSNHQQQPPNSAPALTHQLPPQYASNRVALSHPPQPTSLATSSSAAINIPHSYYSTRGAGGSGFSGPLTTGSIAGPQMQPLMSHNQMIYNPILNPSGSTNPSSHVRTSSGSRQRSGTLDQMDIVPPQLQKVQVDLANYSGQSVTPVLRRDDQYLAWEQRQQHGIGAANSTGIPGNSGLTRRASYRANPQLDYLHEQTYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3K108</accession>
    <name>E3K108_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03939</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP77983.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322402.2">
        <property type="nucleotide sequence ID" value="XM_003322354.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K108"/>
    <dbReference type="GeneID" id="10541291"/>
    <dbReference type="KEGG" id="pgr:PGTG_03939"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03939"/>
    <dbReference type="HOGENOM" id="CLU_121558_0_0_1"/>
    <dbReference type="InParanoid" id="E3K108"/>
    <dbReference type="OrthoDB" id="10269167at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172921" description="Secreted protein" evidence="1">
        <location>
            <begin position="34"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77983.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="152" mass="17270" checksum="873408B9BDC32244" modified="2012-04-18" version="2">MFILNFFRAASVGLAFLPIIIILHSGQVALVDGEQAVNCMPHFDSVDGSPDVSCWVDSHTKLTCPRASCNSRLHPERPYGQIVYNGCQNGDAHKDQQQVIPRNYHHYPTGDNKWYPGQKGFVAAFDDRTQLWYNCAYDFSNENKDTYKKPEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3K1N6</accession>
    <name>E3K1N6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_04167</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178269">
        <property type="protein sequence ID" value="EFP78211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322630.1">
        <property type="nucleotide sequence ID" value="XM_003322582.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K1N6"/>
    <dbReference type="GeneID" id="10542001"/>
    <dbReference type="KEGG" id="pgr:PGTG_04167"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04167"/>
    <dbReference type="HOGENOM" id="CLU_691055_0_0_1"/>
    <dbReference type="InParanoid" id="E3K1N6"/>
    <dbReference type="OrthoDB" id="10648281at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP78211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="399" mass="44129" checksum="F1B16ECA5C4E2058" modified="2011-01-11" version="1">MGQYWFSPWIPHMGPWEVGPGPDSPHHGYIPRSSGGANMEAHRDVEIVRPYFLQVPGTSWGVPLAPTLHPPGGARYLDRYPLTSLPKIKMMVAFYSKKKSKIVPIRCRYITTDIKKCQKSDLWLGTLGVKLGEKERGVPEVPAMGTLGTPREVSGVFSDHQDPYWRVLVEGGMRVSGCGTNVCNEPTCSRPIGMWFFRTIGKKDAQFVELTTKLELTELTKILQSQSCGFHQRWAATLCCTKQSTNASVATLKTVRQTCSVSVLIVPLRYATALLAAKTTKKCCSVANISGVVVKFHCATLNTNGNSAVLRYTNRYEGGQTRKDTVHSGPRPGCTRGFPVETPKVWGFSASNWMPHLPRPIPNPEPVRGLTGFFRIFGVRVGTLVSHTRYISYGNISQS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3K333</accession>
    <name>E3K333_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription initiation factor IIE subunit beta</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_04846</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the TFIIE beta subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323309.1">
        <property type="nucleotide sequence ID" value="XM_003323261.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K333"/>
    <dbReference type="FunCoup" id="E3K333">
        <property type="interactions" value="431"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K333"/>
    <dbReference type="GeneID" id="10536995"/>
    <dbReference type="KEGG" id="pgr:PGTG_04846"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04846"/>
    <dbReference type="eggNOG" id="KOG3095">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_056580_1_0_1"/>
    <dbReference type="InParanoid" id="E3K333"/>
    <dbReference type="OMA" id="RTKKDNH"/>
    <dbReference type="OrthoDB" id="3907302at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005673">
        <property type="term" value="C:transcription factor TFIIE complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054600">
        <property type="entry name" value="TFA2_E-tether"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040501">
        <property type="entry name" value="TFA2_Winged_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016656">
        <property type="entry name" value="TFIIE-bsu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12716:SF8">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12716">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22254">
        <property type="entry name" value="TFA2_E-tether"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18121">
        <property type="entry name" value="TFA2_Winged_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016398">
        <property type="entry name" value="TFIIE-beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="TFA2 Winged helix" evidence="3">
        <location>
            <begin position="150"/>
            <end position="214"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription initiation factor IIE subunit beta E-tether" evidence="4">
        <location>
            <begin position="228"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="243"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="8"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="37"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="256"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="272"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRNR" id="PIRNR016398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF18121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP78890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="307" mass="33662" checksum="E22DB40ABB81FE60" modified="2011-01-11" version="1">MASKFPSLPAPRFAPPKPSPLSQPATTAPPEPPKSQPQPSANAPSSSAPPKKKKKKNAQPTAPPVVEDLGPGRQFATQVFKLVDTLKRTNGPMNLSDLEAQTGVRGLLSEHTDVPFNKELFDAFNSHERVNVTEKGLVRLWSYKPDYVINNPKQVLELLERFSGMGGMPVNTLKQSWPNVMTAITELENEGKVLVLRTESVGAKEGTPKTVFYDQLGCRENLGPTRGALDDEFREMWHSLQTPPVHSLPSELQEAGLTSSTSANLKPQQTTRKPKKKGGKGGKVKITNTHLKDLGIDLSKDYVPYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K493</accession>
    <name>E3K493_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05424</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79103.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323522.2">
        <property type="nucleotide sequence ID" value="XM_003323474.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K493"/>
    <dbReference type="GeneID" id="10530926"/>
    <dbReference type="KEGG" id="pgr:PGTG_05424"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05424"/>
    <dbReference type="HOGENOM" id="CLU_1448378_0_0_1"/>
    <dbReference type="InParanoid" id="E3K493"/>
    <dbReference type="OrthoDB" id="10614636at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="99"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="35"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79103.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="187" mass="20578" checksum="B7AF34233E7A4E8C" modified="2012-04-18" version="2">MPRGRKRPHPGNPAAIASNVEISQDPPSTAPTPTPWNRTQSIKNSQPKDQDLEASTELRMVIGPGVQRSARTRPGLGRVRAKLFIEFFTYARPETTRLKVGSTRAARRAGPSGPDQAPGPLRAELIPRSKECRPVPVWRYPAPVGGYRRHPPRTRQALPDAVPCAHFLEKSGAVPGYRPKVPPAPRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3K6C8</accession>
    <name>E3K6C8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_05084</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EFP79859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324278.1">
        <property type="nucleotide sequence ID" value="XM_003324230.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K6C8"/>
    <dbReference type="GeneID" id="10533550"/>
    <dbReference type="KEGG" id="pgr:PGTG_05084"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05084"/>
    <dbReference type="HOGENOM" id="CLU_033090_2_1_1"/>
    <dbReference type="InParanoid" id="E3K6C8"/>
    <dbReference type="OrthoDB" id="2513067at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="387"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP79859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="387" mass="44747" checksum="3EEED20AA4E018DB" modified="2011-01-11" version="1" fragment="single">MSPSTQTAPVLEALEVLTHAYDRGLWSSYRLNAGEENGELIKDNLDEKNKLMHELQSSLLPSIKHHLETLDPPHDFNDYPSLDIKTTLETLSNLDQTMQKTLHYLNSSPLKVFEPSNAYDHHFKQCKTFRCHALLEPINLLEINLRSLFLAYIKLIESRNLPIKHPEDPRYRAPTPREETVECLKSIDQAIRWSQASDFALLQLKWRGVVERFHDLLARLAKLIDQISRHARINTILRKHIVEQARLIVPVIKLLRNLYDKISNTTKKKLLFTLDLEVNSDTLHTLKVDSGVILCACDMYVQSLELCHMHGSMVDTSEARRVRNKRLSRSVESTLVLLALYIIPLSPKVDHSSLESDFKAWLSQWHVLWRVAKERVLDAMHVHGEEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K6Q1</accession>
    <name>E3K6Q1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CxC1-like cysteine cluster associated with KDZ transposases domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_05396</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EFP80171.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324590.2">
        <property type="nucleotide sequence ID" value="XM_003324542.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K6Q1"/>
    <dbReference type="GeneID" id="10534350"/>
    <dbReference type="KEGG" id="pgr:PGTG_05396"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05396"/>
    <dbReference type="eggNOG" id="ENOG502SMER">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011407_0_0_1"/>
    <dbReference type="InParanoid" id="E3K6Q1"/>
    <dbReference type="OrthoDB" id="2498779at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041320">
        <property type="entry name" value="CxC1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096:SF1">
        <property type="entry name" value="CXC1-LIKE CYSTEINE CLUSTER ASSOCIATED WITH KDZ TRANSPOSASES DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33096">
        <property type="entry name" value="CXC2 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18802">
        <property type="entry name" value="CxC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CxC1-like cysteine cluster associated with KDZ transposases" evidence="2">
        <location>
            <begin position="138"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP80171.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="402" mass="46457" checksum="C61C43A91B6E00D8" modified="2012-04-18" version="2">MPPFEGGGGKKPKQKRKPTTASGVAFSQAVELEQLSTCREATTIHLDHHIPDRSRFDNFDQDHSNPYDHHFTDYTGNDTNFTNSDNTTHSDTRIQQLTDYFRSEHYKRKKLLEEKNWAEVYDAMFSSFKQCAVKTADWGDEEKWRHDWKEPCLCQDKRFRPLVLVDIMSRRETEVEFCSCQGDQVRLIQMGYIGTSPKYPRTAFSIRLLCFHDIIWKNCAVSITPFSKAIDEFLDSNNPLVLVSQTASESGPLYTVREWRKTLSASVDAYREMLRREKVVSEELMEMGPMDKLAEICPKCFGPPVTGKKPEEPDYIVCMDGNFQHRRHLAASHELSEFSNQTSLFISPEEVEDMETCMNIQINEAEEHPDLVSMVTCKSSGSLYRATYCCQRFTRVDHMESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3K8F7</accession>
    <name>E3K8F7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06553</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80597.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325016.2">
        <property type="nucleotide sequence ID" value="XM_003324968.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K8F7"/>
    <dbReference type="GeneID" id="10539609"/>
    <dbReference type="KEGG" id="pgr:PGTG_06553"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06553"/>
    <dbReference type="HOGENOM" id="CLU_1019907_0_0_1"/>
    <dbReference type="InParanoid" id="E3K8F7"/>
    <dbReference type="OrthoDB" id="1898723at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="198"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="198"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP80597.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="273" mass="30560" checksum="3E5BE42FFB188439" modified="2012-04-18" version="2">MIISVTKCRLILGVVCYPAVQDVLARPGLSRESHEIKPLIDYFHEAKPSDPVSEFQASFNNGFKRKRPLEDWHPFPQQHSWPDPIAQLVNPETPLLGQDQEKRARKGIEHEFISDGLESLVSANTFHPHLQGMFGSSSLQDDTNKFPMWENIKKLAQPDLHQEDSHASNALVDNYNEGRLSVAVSELQAIVDGRNDLRRPLQDDYRPQEPLSSTNPIGKVKLEDSSIQNPVERATTDINSVAGSGGWENVVSEGMLQSSSWQGEESTMGNIRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3K8M0</accession>
    <name>E3K8M0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transposase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_06727</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80771.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325190.2">
        <property type="nucleotide sequence ID" value="XM_003325142.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K8M0"/>
    <dbReference type="GeneID" id="10539791"/>
    <dbReference type="KEGG" id="pgr:PGTG_06727"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06727"/>
    <dbReference type="HOGENOM" id="CLU_056788_1_7_1"/>
    <dbReference type="InParanoid" id="E3K8M0"/>
    <dbReference type="OrthoDB" id="2505990at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009057">
        <property type="entry name" value="Homeodomain-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48472">
        <property type="entry name" value="TC1-LIKE TRANSPOSASE DDE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48472:SF1">
        <property type="entry name" value="TC1-LIKE TRANSPOSASE DDE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46689">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP80771.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="143" mass="16482" checksum="36DE374BA0D33CD1" modified="2012-04-18" version="2">MPYVDYPAKFKYKFVRAAIDGMTLSEINTAMASTVSKDSLRRWTNLYETTRAVVCDPATYQTRGRPFELDDEELEFIKEMITEKPTVYLDEIQRALLNEKGTSVSLKTISTTLHQRLKMSKKTIRTVNPCQDSEERALKDFGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3K902</accession>
    <name>E3K902_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06523</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80567.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324986.2">
        <property type="nucleotide sequence ID" value="XM_003324938.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K902"/>
    <dbReference type="STRING" id="418459.E3K902"/>
    <dbReference type="GeneID" id="10539578"/>
    <dbReference type="KEGG" id="pgr:PGTG_06523"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06523"/>
    <dbReference type="HOGENOM" id="CLU_026798_0_2_1"/>
    <dbReference type="InParanoid" id="E3K902"/>
    <dbReference type="OrthoDB" id="2506286at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="1">
        <location>
            <begin position="152"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP80567.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="421" mass="48049" checksum="CFCAFD18C0BB3DF9" modified="2012-04-18" version="2">MMISLNYSCAGVGAHQAYQRQQKRKNNYAHQACRDAQRATVFTSSLHPDTKIMKREYVKYDLYPVRHAKNASTEEVRKTHPTPEEFEAAETALLDRGQFTLIKDGRLVLYEKDVSVLKPGEKRKHKFIAYIEFTSSAYLTTEDGPHKLSTIQLENLSFLSHFLFQCRNYISTVQSVAKIFRGYMWTIGWRKSQTEGEMAGRYVDTGKISSDAFYYMENLMEAFRAGDIIHDLFHSIADTAVLGMKTLLKNCNLPGFNHLNLGNTPVDTDMASNLAFTMNEFSNRPHNDSDVSKHAFLFLTNVDKNTGEIQLNSHLDPSFQGPFFVFPDHRVAVDLRKLDGICRIVFAAGEFRHCTHNNQPTHKKLTSMGFSLQITKSCVDAFKKICNGFYDGKLTKSGKEYYIGDCSHTLKKIKEDHKKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3K993</accession>
    <name>E3K993_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">BolA protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07295</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BolA/IbaG family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP81043.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325462.2">
        <property type="nucleotide sequence ID" value="XM_003325414.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K993"/>
    <dbReference type="FunCoup" id="E3K993">
        <property type="interactions" value="30"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K993"/>
    <dbReference type="GeneID" id="10536282"/>
    <dbReference type="KEGG" id="pgr:PGTG_07295"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07295"/>
    <dbReference type="eggNOG" id="KOG2313">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_109462_2_0_1"/>
    <dbReference type="InParanoid" id="E3K993"/>
    <dbReference type="OrthoDB" id="411584at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005759">
        <property type="term" value="C:mitochondrial matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044572">
        <property type="term" value="P:[4Fe-4S] cluster assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.90">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002634">
        <property type="entry name" value="BolA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036065">
        <property type="entry name" value="BolA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46230:SF7">
        <property type="entry name" value="BOLA-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01722">
        <property type="entry name" value="BolA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82657">
        <property type="entry name" value="BolA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU003860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81043.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="147" mass="16198" checksum="CDFB9845E5FF676D" modified="2012-04-18" version="2">MIGRRASSQALEVLVSRVQRNPALISQELRNQFTRTMSEASSARQTIGPIETTMREKLTAQLNPVVLDIANDSHLHRHHAAMKSIGGGSGETHFSVKVVSDQFVGKPTIARHRLVYQILDVELTKNTAGIHALSLKTLTSEEHEKAP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3KA14</accession>
    <name>E3KA14_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Smr domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_07248</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP80996.1"/>
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        <property type="nucleotide sequence ID" value="XM_003325367.2"/>
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    <dbReference type="STRING" id="418459.E3KA14"/>
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    <dbReference type="KEGG" id="pgr:PGTG_07248"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07248"/>
    <dbReference type="eggNOG" id="KOG2401">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="4080456at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Endo/PolyKinase_Domain-Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002625">
        <property type="entry name" value="Smr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036063">
        <property type="entry name" value="Smr_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46535">
        <property type="entry name" value="NEDD4-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="NEDD4-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00463">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160443">
        <property type="entry name" value="SMR domain-like"/>
        <property type="match status" value="1"/>
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    <dbReference type="PROSITE" id="PS50828">
        <property type="entry name" value="SMR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Smr" evidence="2">
        <location>
            <begin position="455"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP80996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="551" mass="62156" checksum="F15DF46FED51FE93" modified="2011-01-11" version="1">MSQLLERLLAKFSPPLEPALIIALSSDFDHDTQESAEQDIESKLDHILTQLINEQQEQQQLAQETQPEEKQQQLVTIQTDHSSQSQKLINQVQAILPDLSETTIYHAISDAPEPKKLEQIIDILLAPTPPATVESTPRSDKKSKNKRKKKFDTIHLNDVLQRETHKRLIEQAGQHLEKTSINNDWAIGDSQISYLADLLGIEVGKINSIYHRKSCNLVLTLEELLRISESQKRLDGSKDQDWFDRLKLQLDFLRTTLLTEDDQVLTRLLIVTNLNPGHALDLWIFLDDLHARYGTLPFNQFISNPSSSSSSYPSSSSSTAVSLHNQSKANPLISSSSSPTAVASSSSAAAAPSRPFSVNVQPTTRPQYSSREIENSVMILRSRREYLNQKSRSSRQLIANSHAVSHNKQAIQNSIAMIYAEGSRRLNEKIQDWELLLARKTVQERQRIRNDSLSIDLHGLTKPQALIISSDYLQAWWSNHSYSVNNFNSSFVHPFKIVTGAGTHSTNCKPALLPTIMKFLDQEKWKWKADDERSNGLIGSVIVVGRARNRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3KC76</accession>
    <name>E3KC76_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08092</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178280">
        <property type="protein sequence ID" value="EFP81843.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326262.2">
        <property type="nucleotide sequence ID" value="XM_003326214.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KC76"/>
    <dbReference type="GeneID" id="10533879"/>
    <dbReference type="KEGG" id="pgr:PGTG_08092"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08092"/>
    <dbReference type="HOGENOM" id="CLU_826768_0_0_1"/>
    <dbReference type="InParanoid" id="E3KC76"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="259"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="15"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="81"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="155"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="178"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="217"/>
            <end position="235"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="266"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="285"/>
            <end position="298"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81843.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="342" mass="37850" checksum="E72F45D30C5AB18A" modified="2012-04-18" version="2">MSPSTRSGKTYSEASSVTSSRRSSTTRSVHERKRQPWSIAQKDGDPLEAGQINPTVKRQLKPSSVKGNKPNVETNLAEAVRLTRQEKDSFIDSQESTGENRRETIDTIRTNETKAHLDPTTIREGQEETSHSCSHTDVNLGEKTSAVYSRPAENDPTTGAQLPSSTSLQDQSIPIFGRDGRRPTGRSRPERRIGSFDGYRTNDGRPGVQDISAAISHVRDSEEGESARRRNESADRVPTLVPQHLEEANLAEAKSAKLLAKLEGRSQPTASGSQPSGSYNSPNNRQEKRPAETDVNRLGKIAKLDTSWRETMAAARALQQARAQVLEELRKEEEASKRRKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDG7</accession>
    <name>E3KDG7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_08359</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326822.1">
        <property type="nucleotide sequence ID" value="XM_003326774.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDG7"/>
    <dbReference type="GeneID" id="10529781"/>
    <dbReference type="KEGG" id="pgr:PGTG_08359"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08359"/>
    <dbReference type="HOGENOM" id="CLU_1504162_0_0_1"/>
    <dbReference type="InParanoid" id="E3KDG7"/>
    <dbReference type="OrthoDB" id="10295162at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173253" evidence="2">
        <location>
            <begin position="19"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="92"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP82403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="176" mass="17322" checksum="CE5ACD87A6D1FABE" modified="2011-01-11" version="1">MQSSLVLSVLLICSGVIAVPISGLTDFTEIESFQKQGSNVPTHLLNPTSPQPHSLQAQHPPQSGKPSFPVQPAPQHLSDVAQSDVVHAPVPISSSPAPGGTNPQSGSVVNGGGMPAGFASNVARLVGGSTAFGTGTTSNVISGGHKGEPLVNGAGAIADATVGVAGQAAGMPKPNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDJ9</accession>
    <name>E3KDJ9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_08391</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82435.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326854.2">
        <property type="nucleotide sequence ID" value="XM_003326806.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDJ9"/>
    <dbReference type="GeneID" id="10529814"/>
    <dbReference type="KEGG" id="pgr:PGTG_08391"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08391"/>
    <dbReference type="HOGENOM" id="CLU_033090_3_1_1"/>
    <dbReference type="InParanoid" id="E3KDJ9"/>
    <dbReference type="OrthoDB" id="2495413at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069">
        <property type="entry name" value="CHROMOSOME 7, WHOLE GENOME SHOTGUN SEQUENCE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33069:SF3">
        <property type="entry name" value="DYNEIN HEAVY CHAIN TAIL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP82435.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="408" mass="47030" checksum="BBA896226DA0C473" modified="2012-04-18" version="2">MDDPDAQLEAQLEAWYNGLVDSPEPDTPEMRQFDWKQGDLAIKGFELLSAHLFHWPSTFTRDTPADLSIGRLRSKKDIHTQLHSSLLPLLRQHLNSIAEALDDLIELRRDPAPKIQRVLELQPNLHRILDQIIRTLDEIIPGKIAEPSQTNDQHFEEFKTYRLYGLDDCIRDRLEFGIINFLSNSRGIIEELKLPARKRFPTSIGGLCFFNSAFNQTIKWLKGSELHIIPDLWEIPIRDTYEAHEEYFRLTHPEADPPMSHAAIQLARSIIPIIKLSKLFFQKLLREGLMERKQVPLFTEMCSHQMYSLHKSSEEYGESVMSLVFRLEDAHLDPPADTSSSLIQNIQALSSHFQSYVSLAELYMTPLFPDINGVSAQIYFKTWFITWNTLFLTATQNAIQAANVFAQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDN5</accession>
    <name>E3KDN5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_08427</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82471.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326890.2">
        <property type="nucleotide sequence ID" value="XM_003326842.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDN5"/>
    <dbReference type="GeneID" id="10529850"/>
    <dbReference type="KEGG" id="pgr:PGTG_08427"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08427"/>
    <dbReference type="HOGENOM" id="CLU_2489507_0_0_1"/>
    <dbReference type="InParanoid" id="E3KDN5"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP82471.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="102" mass="11341" checksum="450C4FCA6978DAAF" modified="2012-04-18" version="2">MATEECSSGTWMAFAMRGECLSSLAKSVLDSNDGSLMNNDTQKVYLKTTLQLAPNYPPTPGPNLSPCATASFRKIVTLSWIFQAEIKHFFLKENPHQGRQPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="89" xmlns="https://uniprot.org/uniprot">
    <accession>E3KF80</accession>
    <name>E3KF80_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Histone-lysine N-methyltransferase, H3 lysine-36 specific</fullName>
            <ecNumber evidence="4">2.1.1.359</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">SET domain-containing protein 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="21" type="ORF">PGTG_09875</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="22" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Histone methyltransferase that trimethylates histone H3 'Lys-36' forming H3K36me3. Involved in transcription elongation as well as in transcription repression.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-lysyl(36)-[histone H3] + 3 S-adenosyl-L-methionine = N(6),N(6),N(6)-trimethyl-L-lysyl(36)-[histone H3] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:60324"/>
            <dbReference type="Rhea" id="RHEA-COMP:9785"/>
            <dbReference type="Rhea" id="RHEA-COMP:15536"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:61961"/>
            <dbReference type="EC" id="2.1.1.359"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EC" id="2.1.1.359" evidence="4"/>
    <dbReference type="EMBL" id="DS178284">
        <property type="protein sequence ID" value="EFP82907.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327326.2">
        <property type="nucleotide sequence ID" value="XM_003327278.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KF80"/>
    <dbReference type="FunCoup" id="E3KF80">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KF80"/>
    <dbReference type="GeneID" id="10544618"/>
    <dbReference type="KEGG" id="pgr:PGTG_09875"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09875"/>
    <dbReference type="eggNOG" id="KOG4442">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008492_1_1_1"/>
    <dbReference type="InParanoid" id="E3KF80"/>
    <dbReference type="OrthoDB" id="422362at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KFL6</accession>
    <name>E3KFL6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_09033</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP83080.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003327451.2"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172587" evidence="2">
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            <begin position="28"/>
            <end position="168"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="118"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="138"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP83080.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="168" mass="18830" checksum="E878DDCC64B1E0CE" modified="2012-04-18" version="2">MLFIIQAALAALLVQDFLSFCSGGLQGEDVVESTDDLAPPACGNCRLTDRVVGTPTDRHCPDLRDCQRHTCLQYQTGIVFRCRRCLEWPGLTKWHPCDDKQIYHDHCATCQPPSVIDPPWPMISPSDNHPHRVHRPSSSRAPSASSSAASNSVQVDPHLRNIRFYHFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGJ1</accession>
    <name>E3KGJ1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF6589 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08602</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327835.1">
        <property type="nucleotide sequence ID" value="XM_003327787.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KGJ1"/>
    <dbReference type="GeneID" id="10545133"/>
    <dbReference type="KEGG" id="pgr:PGTG_08602"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08602"/>
    <dbReference type="eggNOG" id="ENOG502SCRC">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_009176_0_0_1"/>
    <dbReference type="InParanoid" id="E3KGJ1"/>
    <dbReference type="OrthoDB" id="2495569at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046496">
        <property type="entry name" value="DUF6589"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20231">
        <property type="entry name" value="DUF6589"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF6589" evidence="1">
        <location>
            <begin position="360"/>
            <end position="650"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF20231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="662" mass="74185" checksum="BD00A0C8EED73BBB" modified="2011-01-11" version="1">MDPQNVPILTEAEKVIQICDAIHHLQMTPKKFINAFLTNADPQIAYKRRFWGTSTGWSSTHGIIQAVKAEVARTRDGADRWDAFYTQSVEILLSQSPPRGNFPLGSFHSSRTVQPSFFDPKAVSERDEALTKNHMPFLFNLIKRMLVRVEAPDELDEDGPVEPTIADPEQDHILNMDNVTYVADRNSAFSIRIHKACFSFGFVQVEANHQQPVASTICSMVAFTRNRRHNSMQLYNSIRFVAGGLSDQLNDYLHLLGITSSRQTAIRALTFLSTCAARDLRNAMTPMAGVPVGPSICIDNLDMEQRVHAHSIGHRTMMFHGSWGYIHHPHPSLLDSLDLSELSLKSYYESLQKVSSLIIQPSMFLPTADEDLHFEAVLKSQIARVMRHYIAEPSTRDGSIQIHPPSIDPIDCSPPNIKMLKLMDASDNSAEGIGQVIESIISQSGLTAEEFCSRLQVMDGDLGTIQNFNSLRALRTPSAHPEHNLHNISFQLGASHTLWNIAQNILTAHFGDPTKTNDLGAWHDLHALGIPSDKAVPKKDFDQMINNMEKIHEASIFHCLCVIMGTTRDQVAKTPGKIETRKWNQIIEACYDRFFSPEARRSTSKEASPKLFALLHRLHDFSTVIEANRAMKAGDIGRLVNIWKIWCIMSQALTADTSSTTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KH62</accession>
    <name>E3KH62_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_09350</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83637.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328056.2">
        <property type="nucleotide sequence ID" value="XM_003328008.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KH62"/>
    <dbReference type="STRING" id="418459.E3KH62"/>
    <dbReference type="GeneID" id="10532323"/>
    <dbReference type="KEGG" id="pgr:PGTG_09350"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09350"/>
    <dbReference type="HOGENOM" id="CLU_004591_5_1_1"/>
    <dbReference type="InParanoid" id="E3KH62"/>
    <dbReference type="OrthoDB" id="2288618at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP83637.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="464" mass="53107" checksum="71C30E02A4EF8331" modified="2012-04-18" version="2">MFALDAREIISDDLRNPLVAPHLDFLPEDAHGKGIFKTSQSAKWLKHLAPDIRVQMVAYNKKHYYTYEPVQLKNDNIIIPIFFYTLQSNTFAKCYKPQITSNRHNSTVKIAIASDILYDDPDLQEINLDDFDLLYSEIVSSDGLKLLEYCGNLLYEQNSEGTIKLPNPWREKANGRIIRHVPVNMYCDDTSGNKSKKWNKHISYYFTLSGLPPNLSNQQFNCHFLCTSNIAGALDLGEIVVQQLNEMVNDGFEAYDMILSQNVLVMAPLLCFLANSPMQAEITNTPVPGPSLNPCRVCVLSSKTIKDRNTLGYISRFVNKNTHGSNHPNNLRTTEKTIKDSEEVWRYVKENALTLNLEKLNEKSAEFGIRDQTNRKFAKQIISFREKKKELLLDGKEIPPDMDQPIPQDLVDLESKDKTQVLSPFLKLKGLCFDMVRDTPVEILHVFLLGIVKYLFTEFMSRLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHM2</accession>
    <name>E3KHM2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Trafficking protein particle complex subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_09510</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Part of the multisubunit transport protein particle (TRAPP) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Endoplasmic reticulum</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Golgi apparatus</location>
            <location evidence="7">cis-Golgi network</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Golgi apparatus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TRAPP small subunits family. TRAPPC4 subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83797.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328216.2">
        <property type="nucleotide sequence ID" value="XM_003328168.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHM2"/>
    <dbReference type="FunCoup" id="E3KHM2">
        <property type="interactions" value="23"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KHM2"/>
    <dbReference type="GeneID" id="10532484"/>
    <dbReference type="KEGG" id="pgr:PGTG_09510"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09510"/>
    <dbReference type="eggNOG" id="KOG3369">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053380_2_2_1"/>
    <dbReference type="InParanoid" id="E3KHM2"/>
    <dbReference type="OrthoDB" id="246406at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030008">
        <property type="term" value="C:TRAPP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14856">
        <property type="entry name" value="TRAPPC4_synbindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.70:FF:000007">
        <property type="entry name" value="Putative sybindin-like family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007233">
        <property type="entry name" value="TRAPPC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23249:SF15">
        <property type="entry name" value="TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04099">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01399">
        <property type="entry name" value="Sybindin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4 7" id="KW-0931">ER-Golgi transport</keyword>
    <keyword evidence="5 7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU366065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP83797.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="137" mass="15113" checksum="369D6F0EB845BC1B" modified="2012-04-18" version="2">MSNTTFSLWIINKAGGLIYQKTYAEGLLPQLTSNDYLVLAGTFHGIHAITSKLSPVHPSGGGVETVEAETFKMSCFQTLTGTKFVLFTSPSHPSPSRLLSRIYGVYADLLKDPFYSVENPIRNETFDKRCQSICSTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHX7</accession>
    <name>E3KHX7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">RanBP2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09615</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EFP83902.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328321.2">
        <property type="nucleotide sequence ID" value="XM_003328273.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHX7"/>
    <dbReference type="GeneID" id="10532592"/>
    <dbReference type="KEGG" id="pgr:PGTG_09615"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09615"/>
    <dbReference type="HOGENOM" id="CLU_148840_0_0_1"/>
    <dbReference type="InParanoid" id="E3KHX7"/>
    <dbReference type="OrthoDB" id="2508453at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172016" description="RanBP2-type domain-containing protein" evidence="1">
        <location>
            <begin position="19"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83902.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="131" mass="14599" checksum="666B5C059A0A101D" modified="2012-04-18" version="2">MQLHTGRAALICAVFVSAMTMCSSPPRMPARLPRPLRRGNSNRICANCVTEGYEVIDGQRCLYELPSPARRPCQEILEPIRGEPPCPGIITGKEWRCDKCNCRLFESAQGCLTCESGKAKRYFPQSRAPPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3KI04</accession>
    <name>E3KI04_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Conserved oligomeric Golgi complex subunit 3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Component of oligomeric Golgi complex 3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_09642</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the COG3 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EFP83929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328348.1">
        <property type="nucleotide sequence ID" value="XM_003328300.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KI04"/>
    <dbReference type="FunCoup" id="E3KI04">
        <property type="interactions" value="638"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KI04"/>
    <dbReference type="GeneID" id="10532621"/>
    <dbReference type="KEGG" id="pgr:PGTG_09642"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09642"/>
    <dbReference type="eggNOG" id="KOG2604">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011639_1_1_1"/>
    <dbReference type="InParanoid" id="E3KI04"/>
    <dbReference type="OMA" id="DEFELWG"/>
    <dbReference type="OrthoDB" id="296793at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005801">
        <property type="term" value="C:cis-Golgi network"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017119">
        <property type="term" value="C:Golgi transport complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007030">
        <property type="term" value="P:Golgi organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006891">
        <property type="term" value="P:intra-Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048685">
        <property type="entry name" value="COG3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048320">
        <property type="entry name" value="COG3_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007265">
        <property type="entry name" value="COG_su3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13302">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX COMPONENT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13302:SF8">
        <property type="entry name" value="CONSERVED OLIGOMERIC GOLGI COMPLEX SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20671">
        <property type="entry name" value="COG3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04136">
        <property type="entry name" value="COG3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="7" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 3 N-terminal" evidence="10">
        <location>
            <begin position="131"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="domain" description="Conserved oligomeric Golgi complex subunit 3 C-terminal" evidence="11">
        <location>
            <begin position="300"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="66"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="507"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="777"/>
            <end position="797"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="72"/>
            <end position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004395"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF04136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF20671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP83929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="926" mass="106001" checksum="EBFF5F0596050C38" modified="2011-01-11" version="1">MSSSQQPSTSEQQQQQLKHQQNLIQHWDLLSPLSDLEKQSIHSIQKASNDKPLPSSISLRNQSLNNLNHTQLSPPSSTRSNSPSSSLRRLINHSKPLEHHQPISSIDRFNDWFSTISSQIEADSESAYLNHLSVLSTYSEACDLLDQAIDDCRACLNEIEANWKFVNENSKSLERSCQGMLDDQKVLGSIATALNDRLAYFRRLDESQRVLSRPGEAEIVNSQEFLPMIERLDVCLEFMKVNRHFRDADLYLVRFQQCLTRSMTLIKLYYTNQMKNLSRQVQEKLQINQQSSSNLKIQDTLLYAKFQTLSEELRPLISQVEKRYLFDQDEYGALLSEFFSTWVSVRSQLLHARIKIEISRIQITHQQVPDLISLATTGCNYMRSVCTAEWNLFKSFFPKTGEEELFVYLESLCDYLYDLLRPQILHEQKLYLLCDLATISNALIAMDSNLIEANQSQFKFSTLLKPVLQDIQTRLVFRAQSIIQSEVANYLPKSEDLDYPNKLIQADQKRQQSGQKSKRFNNPHPTLLDRPHAPLKSRLPAEGVQENWYPTLRKTLWVLSRLHTYVNDAIFEDFAGEAVGICSESIIKASNMMNSVVTQQAGSTTPAMSSKIQANSIDRELFVIRHLLILKEMIRTLDVVQVERAVDLGPITDVLKEILNPSFKDSLIFKPITLMNKLKNLNHSQIEFSKRMGYSSQKDQDSSNPAEPNLSLNFDMLDAQITKTIDSKSEVDLKLKTICHQFIIKSSALICSNSLEPFLTKCQNHLNLEASMVPPSSAADPLGGGGGGGNSNLNRNLGDQEWASQDNVLAVFQAFLKDLQLGLNIIFNRVALYFHDDDHHDFNLINVLFPSLQEEIMEKYRRFYSLIKFEFDFNFINDDQISKDKDQDPEQEIDYESLNQAQSRVLAPSQLHSLIQKIVYRQSSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJ18</accession>
    <name>E3KJ18_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">UDP-galactose transporter homolog 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_10671</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endoplasmic reticulum membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the nucleotide-sugar transporter family. SLC35B subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328712.1">
        <property type="nucleotide sequence ID" value="XM_003328664.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJ18"/>
    <dbReference type="FunCoup" id="E3KJ18">
        <property type="interactions" value="401"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KJ18"/>
    <dbReference type="GeneID" id="10545637"/>
    <dbReference type="KEGG" id="pgr:PGTG_10671"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10671"/>
    <dbReference type="eggNOG" id="KOG1581">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_036019_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJ18"/>
    <dbReference type="OMA" id="CGAIGQV"/>
    <dbReference type="OrthoDB" id="1601at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005459">
        <property type="term" value="F:UDP-galactose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005460">
        <property type="term" value="F:UDP-glucose transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072334">
        <property type="term" value="P:UDP-galactose transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120112">
        <property type="term" value="P:UDP-glucose transmembrane transport into endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013657">
        <property type="entry name" value="SCL35B1-4/HUT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10778:SF10">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08449">
        <property type="entry name" value="UAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0762">Sugar transport</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="168"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="197"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="236"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="340"/>
            <end position="359"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP84293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="382" mass="41713" checksum="5117B00B712EA5AA" modified="2011-01-11" version="1">MAFVRLVICVVGIYASFLSWALIQERLSTTPYEDPSGGKPKYFKSVIFLNTIQSGFSATAALIYLLLKKNSSQSILQVLGLTNPTPSKAEKTPKQPNSRLAPKDQNRLALLGRCLQCAILNSLGSPFGYASLKHIDYPTMILGKSCKLVPVMIMNIILYRRKFAIHKYIVVGMVTVGISLFMLFADHGSKASKGAQQSSLFGLTLLLINLLIDGATNSTQDEIFSRFTISGSQLMFIMNALATIITLAALQAPLPTAISSFFGTSNSSGNEFTVAIEFIKSYPKVLQDILLFSSAGAIGQLFIFETLSHFGSLTLVTITVTRKLFTMLLSVFFFDHKLSFGQWGGIAIVFGAIGLEAYVKMSGHGKANLIIDEKQKAKMKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJ55</accession>
    <name>E3KJ55_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Deacetylase sirtuin-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_10050</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the sirtuin family. Class I subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178290">
        <property type="protein sequence ID" value="EFP84330.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328749.2">
        <property type="nucleotide sequence ID" value="XM_003328701.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJ55"/>
    <dbReference type="STRING" id="418459.E3KJ55"/>
    <dbReference type="GeneID" id="10545676"/>
    <dbReference type="KEGG" id="pgr:PGTG_10050"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10050"/>
    <dbReference type="eggNOG" id="KOG2683">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_023643_3_2_1"/>
    <dbReference type="InParanoid" id="E3KJ55"/>
    <dbReference type="OrthoDB" id="424302at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004407">
        <property type="term" value="F:histone deacetylase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003950">
        <property type="term" value="F:NAD+ poly-ADP-ribosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1600.10">
        <property type="entry name" value="SIR2/SIRT2 'Small Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050134">
        <property type="entry name" value="NAD-dep_sirtuin_deacylases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003000">
        <property type="entry name" value="Sirtuin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026591">
        <property type="entry name" value="Sirtuin_cat_small_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026590">
        <property type="entry name" value="Ssirtuin_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11085:SF10">
        <property type="entry name" value="NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02146">
        <property type="entry name" value="SIR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50305">
        <property type="entry name" value="SIRTUIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Deacetylase sirtuin-type" evidence="6">
        <location>
            <begin position="23"/>
            <end position="359"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="162"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP84330.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="359" mass="39641" checksum="09042F305CC663B1" modified="2012-04-18" version="2">MPMGRISVPKIPIGDPLNKRAVISDISTAVSYVIRLLDHGAGNVLAISGAGMSVDSGIRAYRGAKGSYIINKSYRPIFFHEFLESHSFRIRYWARSYLGYPTVRVAEPNVGHYAVAALMKMGYIRHLITQNVDRLHHKAIGVKQDSHAPDYFNPHPNILELHGTLKHVNCLSCGHQIDRDDFQDLLSELNPSWKNYADTLQSIGQEVKTNPDGDADVKDKSFDSFVVPNCEKCTVGLLKPAVTFFGESLSKEAKELANRRVSEATNLLLLGTSLTTYSAFRLVKELKEKAGSQIGMINIGTTRADELIDWKIGSHLDTGGLGCSQVLKEVGLQLAARQRLPDSHPVIELLHSGVMKPLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJI3</accession>
    <name>E3KJI3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_10178</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178290">
        <property type="protein sequence ID" value="EFP84458.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328877.2">
        <property type="nucleotide sequence ID" value="XM_003328829.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJI3"/>
    <dbReference type="STRING" id="418459.E3KJI3"/>
    <dbReference type="GeneID" id="10545968"/>
    <dbReference type="KEGG" id="pgr:PGTG_10178"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10178"/>
    <dbReference type="HOGENOM" id="CLU_2307424_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJI3"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP84458.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="100" mass="11048" checksum="14572982060B88E0" modified="2012-04-18" version="2">MLPQIYGKSTYLSLGEKGPVTPPQKYKWLHDTLPSQQTDFGKGGLLGDNTNLTQTPAQLEATKAQYSSPDSMPKMCQVSIKSPLRTPKRTQANALLQQAW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3KK86</accession>
    <name>E3KK86_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329130.1">
        <property type="nucleotide sequence ID" value="XM_003329082.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KK86"/>
    <dbReference type="GeneID" id="10526678"/>
    <dbReference type="KEGG" id="pgr:PGTG_10870"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10870"/>
    <dbReference type="HOGENOM" id="CLU_1046394_0_0_1"/>
    <dbReference type="InParanoid" id="E3KK86"/>
    <dbReference type="OrthoDB" id="10508949at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="45"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="195"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="22"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="220"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="266" mass="30462" checksum="C15B0B702450FD98" modified="2011-01-11" version="1">MGQWCSTQGPPKAPFFSRRKSTRDDTLRGLLDEGNPMRKFAMWTAGSKKVTSKKRMRSDLELQGSDEAPSKGIGQPPKKKTKYAVAALINRDVCKGSLALNHTYIAEELRAMYKAARSKSTPSNKKCHWPGTETVLHLARINHKLIVHENPVGLSVRHLIDVPIKKMSIDDTWLVLRGLKNKWIDLVEHQFDSLPKPTHARKQQKQKETGASATIVREEEKEEEEGEEEEEEEEDDEDEEEEEGEDDEDEEDEDDDEDEDDDEDDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KKD8</accession>
    <name>E3KKD8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_10922</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329182.1">
        <property type="nucleotide sequence ID" value="XM_003329134.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KKD8"/>
    <dbReference type="GeneID" id="10526732"/>
    <dbReference type="KEGG" id="pgr:PGTG_10922"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10922"/>
    <dbReference type="HOGENOM" id="CLU_072136_0_0_1"/>
    <dbReference type="InParanoid" id="E3KKD8"/>
    <dbReference type="OrthoDB" id="10272563at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172677" evidence="2">
        <location>
            <begin position="24"/>
            <end position="333"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="144"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP84763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="333" mass="38097" checksum="F566B8CB8815481D" modified="2011-01-11" version="1">MRPQSRPLLRVLSILHLIYLTNTVARGGLLPPKDHHLKKRCLPLPNCVTEIMGYFFEKAQGVSDQLSVVSQETAQQRWNAELVKERASSPLVERSYWEHRTFQVCQNAALNELLKESARSRIDQATIIALLSKKLIHPIEQNHRPDRLPVSYPPGLREPPSEASSPRNKDNVWDKNLEENHEIIHPESPASDPLPLGFAAVHENIKSMKSECENLSAFNLETSQWSSIILRLMVVTLAHKVFESAQWKVGREIIFEELISLAYLERTSIYREIVTGEQAAQDLLKEEDWWSKVKGGMSDNLSQIIGPETERSDSQDEWWMALKEKLSHKESIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3KL43</accession>
    <name>E3KL43_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_11187</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP85018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329437.1">
        <property type="nucleotide sequence ID" value="XM_003329389.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KL43"/>
    <dbReference type="GeneID" id="10526995"/>
    <dbReference type="KEGG" id="pgr:PGTG_11187"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11187"/>
    <dbReference type="HOGENOM" id="CLU_069433_0_0_1"/>
    <dbReference type="InParanoid" id="E3KL43"/>
    <dbReference type="OrthoDB" id="10467719at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP85018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="272" mass="31185" checksum="1E52B7FF41C91869" modified="2011-01-11" version="1">MTIKLSYLLYKERRSGDPLARPHFFKITPTESVRNWTGNISEASFEEFKKTLFAHVTTSRKIRRILDMGNCSGRLYFYVSAHLGQPLGVCCPLHGIHFCSFASFVAEAMRQPERPITIIMSMRHVGRHCVADGTEVADHFSGGSFFVLSERNKCPGREHQFHTDRQGFRLQPHLVPSRIKPEIGPLGFVEELLVFCGIPQNDRLTHGLIDLHQIDDWRFFVGRSCEELEDLGFGTRTIQLLADGVPMYIVHLNDTLFDTSDEDEFIDYDDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3KM85</accession>
    <name>E3KM85_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_11766</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178295">
        <property type="protein sequence ID" value="EFP85410.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329829.2">
        <property type="nucleotide sequence ID" value="XM_003329781.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KM85"/>
    <dbReference type="GeneID" id="10543964"/>
    <dbReference type="KEGG" id="pgr:PGTG_11766"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11766"/>
    <dbReference type="HOGENOM" id="CLU_011027_2_0_1"/>
    <dbReference type="InParanoid" id="E3KM85"/>
    <dbReference type="OrthoDB" id="1612078at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021102">
        <property type="entry name" value="PNGase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056948">
        <property type="entry name" value="PNGaseA_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31104">
        <property type="entry name" value="PEPTIDE-N4-(N-ACETYL-BETA-GLUCOSAMINYL)ASPARAGINE AMIDASE A PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25156">
        <property type="entry name" value="PNGase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12222">
        <property type="entry name" value="PNGaseA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172720" description="Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A N-terminal domain-containing protein" evidence="2">
        <location>
            <begin position="24"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="domain" description="Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A N-terminal" evidence="3">
        <location>
            <begin position="57"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="615"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="616"/>
            <end position="626"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="638"/>
            <end position="648"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF12222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP85410.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="648" mass="70389" checksum="E90C5433C3C8E8EA" modified="2012-04-18" version="2">MFRISLPWIALWALLSASDLVHGTSLQSSSKQLHARQAVHRPNPLRNFEVREPPSVPKSQPCEVAIVEHTFVTSWGKPANASFTPPKKCGRPGSWASVIFNLTATSIGTQYDRVGMLYLNDIDTEPTKEGIVWTVTRDMTKYIPLLSGPASFSLDIGNTVDQSLDLTGSFEVTLSAKFYPPSLEFPAAKQADRILNMGHGSGNNMTSFMSFPQNIATAYLELFASGRGKEEYTNVPDVYRSKLDPTNAGTVTGKGSFREVQVWIDHRLAGVAYPFPVIYTGGILLAWWRPIAAIGAFDAPTYVLDISPFVPLLADSKPHNFTLRVVGQGENGSINEDWVFSAAVFVGLDPSATRTTGSILTHFTDSKTTVEVPKDIRIPPNLDPKSLTSFTTRSFRQLSISSSVVTGTGELKIVKVEQDMTFVNQQTWAADTAYQSIVMTSQGNSISTHGDTAEKIDNFRYPFNLTLSALAVPSATKIVGHLNHKYSRSQVFPFSSSLGKIAIETTQDAAGELVVDQKGRALSGLGRTIQSFTYKDGKGGSYARDVDIYNSTKTIRDKESGTLLPAIKTESLTTLSVSNTPLETSLLSTDSLSTNGPAPKGLPENAFSTSAISFSEDQHLHGDETLRGAPCKTLHTPAPRPKSKTPPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3KM98</accession>
    <name>E3KM98_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11779</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178295">
        <property type="protein sequence ID" value="EFP85423.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329842.2">
        <property type="nucleotide sequence ID" value="XM_003329794.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KM98"/>
    <dbReference type="STRING" id="418459.E3KM98"/>
    <dbReference type="GeneID" id="10543974"/>
    <dbReference type="KEGG" id="pgr:PGTG_11779"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11779"/>
    <dbReference type="eggNOG" id="ENOG502S6K1">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_008138_1_0_1"/>
    <dbReference type="InParanoid" id="E3KM98"/>
    <dbReference type="OrthoDB" id="107110at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266">
        <property type="entry name" value="CHROMOSOME 15, WHOLE GENOME SHOTGUN SEQUENCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33266:SF1">
        <property type="entry name" value="F-BOX DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="798"/>
            <end position="850"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="799"/>
            <end position="816"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85423.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="922" mass="103924" checksum="79090F619E97A626" modified="2012-04-18" version="2">MTKGLRHPLIYSNRAGLDSLTEKSNFTDSSPSGISKRTALHVDVENAEEFLMKAERQVVEKYVDKLERLKSALTTNTAHGTIEVDDLRFQLGWTRVLNRKYAFQALVDFWFVDDKANLQEEISELALLLPHPEFTRHAKTTHDTRVTAINSSSSREARLVLAESMTFQTVQREIVLQGYEHTYKNSDDIVIPTLNTLGKYASKWSSPEYLAPCTELIGPSMNGKTRLLMELSNHVCVAYICVRPNGSSGHPPRSQYASEILLDTNSSALRLQTQYEDLLLAIFNVVADFFHAHQRLSMQEKMTKWISHSFPRKNQVGDPPFWTDVKNKMKEISNSPDSDPDRSKQFAQASARLQQNTDFIKAENLRVLLAIDEARELLKSATSSDFSFFRIFRHALKKIPYKSNIFVVFADTTSRVSNFNPPAHKDPIHRIGMENGKVEKLYPPIYEIPTFDLHVAEPPTTWCQLQSAFRLFCYGSPFWSLYAADAQREGVAHRQIVLDLIPFALQKLLCTSTASIPASSLTDPQAIALLGSTIQPQLYGASQLNSKLVSSHAAQCMYIDPLRQMLISEYPSQFTFSSAANEYLAYDDERLIRCIKVLASTRRQGHLGSGDAGELVSRIILLRAMQVTMQKTQPTPAPDANPEMITMPFGHSVGLQDFLKTLTDSQEVEKLNLGTISEQCKKKLLNEGHIFWNHFISVDHTPTSADLLQKLYRGLAINCKPNQPGFDQMFTIYLQSQPTATLDKQGITFCGIQVKNRKQNDKLEDDSYKWTPTFAGIKLKEEHLYLVLYLSLRDARPKPTSSIKSQPTASQPSRTQPPRKKSTGKQPARTNQTTSQPPPSQPNLQPKIIPIPQGTLSKEESERRASLAFYGLDSFPFLTQGLVDALEELLDAYPNIQFLHQESSGQAKKFLKQVSPEVSSLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KMC3</accession>
    <name>E3KMC3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11804</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178295">
        <property type="protein sequence ID" value="EFP85448.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329867.2">
        <property type="nucleotide sequence ID" value="XM_003329819.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KMC3"/>
    <dbReference type="GeneID" id="10543999"/>
    <dbReference type="KEGG" id="pgr:PGTG_11804"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11804"/>
    <dbReference type="HOGENOM" id="CLU_2513745_0_0_1"/>
    <dbReference type="InParanoid" id="E3KMC3"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85448.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="85" mass="8885" checksum="4AC1388C9B200096" modified="2012-04-18" version="2">MHLLYSFPSINHACRSLHAQITHPTRKSTTHKTAPAAPTTTPNTASTTTSTAAKTAATAEVPAWAGAPGPAKEIPTSFHPKCHSN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KN22</accession>
    <name>E3KN22_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">ATP-dependent DNA helicase sgs1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_10987</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EFP85658.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330077.2">
        <property type="nucleotide sequence ID" value="XM_003330029.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KN22"/>
    <dbReference type="GeneID" id="10544224"/>
    <dbReference type="KEGG" id="pgr:PGTG_10987"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10987"/>
    <dbReference type="HOGENOM" id="CLU_983970_0_0_1"/>
    <dbReference type="InParanoid" id="E3KN22"/>
    <dbReference type="OrthoDB" id="2507066at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="279"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="228"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="281"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP85658.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="312" mass="35210" checksum="FCB430F070055189" modified="2012-04-18" version="2">MSDAGTMQTRRLGYVPLSVDDPNYKKEAARETEKEFAECFCSNCKPESSQWLINNMKRATIKNFDSFISDLREDIPELNPIIRSCSVQTNKVVWVEESGKKPLEESLEMFSKDLVGYFNRLFESVMKNMATYPPNAYFCIKQARQISKNIGQLTIDNIEELIGGEMLPGQMSILIKHIELFRSGELFKCVAQQQEEAKTQLNLPAERLRLARETEENKRIEKQKAIHVADALKRAETAERVAKNAERKRVAKEAKFVESEKKQAKRDSDMAILQELKAQAQAASELSRSQDAVGPTPSTSGAVNGSIDPSLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNB3</accession>
    <name>E3KNB3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Chloride channel protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_11078</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the chloride channel (TC 2.A.49) family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EFP85749.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330168.2">
        <property type="nucleotide sequence ID" value="XM_003330120.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNB3"/>
    <dbReference type="STRING" id="418459.E3KNB3"/>
    <dbReference type="GeneID" id="10544315"/>
    <dbReference type="KEGG" id="pgr:PGTG_11078"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11078"/>
    <dbReference type="eggNOG" id="KOG0475">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_003181_2_2_1"/>
    <dbReference type="InParanoid" id="E3KNB3"/>
    <dbReference type="OrthoDB" id="44789at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd03684">
        <property type="entry name" value="ClC_3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3080.10:FF:000024">
        <property type="entry name" value="Voltage-gated chloride channel, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1280.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45711">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45711:SF6">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7 9" id="KW-0868">Chloride</keyword>
    <keyword evidence="5 9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="148"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="228"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="274"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="330"/>
            <end position="354"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="409"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="450"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="497"/>
            <end position="515"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="527"/>
            <end position="550"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="570"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="594"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="11">
        <location>
            <begin position="660"/>
            <end position="721"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="11">
        <location>
            <begin position="811"/>
            <end position="866"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="1"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="733"/>
            <end position="798"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="34"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="746"/>
            <end position="764"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="781"/>
            <end position="796"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP85749.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="874" mass="96052" checksum="EE4669FB024B9902" modified="2012-04-18" version="2">MGNHQGSSTQSRRGTHSSRSGSPVSSISYDSAARTPTKNQGSGSRLFSPFRKKDTLRGLSSPISLRLLGSSAALDVGEGPRRDEFWADGQWWRGGRDGRPAEGQRVERSYADRTMIDWAYEDRKERNRLQKLDRLPGLRGILARLVNLTWPWIGVILVGAIVGLVAGSLDTLALWLSDLRDGNCDYAFYLNKNACCSGLEPHEICYEWKTWPQVFKISSAPIASFSHYLAYITSSVMFAGVVAFLVKSFAPFAFHTGIPEIKVILSGYTFQHYLSAWTLVIKAIGLAFAVGSGLSLGKEGPLVHVACCVANLVLQNFKVFRTNEARKREMLSAAAASGVSVAFGAPLGGVLFVLEELSLASLPQPTLWRAFVCAIVATMTLQSFDPFNSGKLVLFQVQSVGQVWRTFELIPWVFVGLCGGLFGATFIRANIEYAKIRRSSGLADHPIKEVLAVAGFTALVSYLLLITRLPTSQLVEALFQECGASNLDVFSFCDSNQVFSTVMLLLIASVAKAVITSMTFGIQVPSGIFLPAISIGACFGRAIGMVMHSWQQAYPRFWLFGSCPPEGTCISPQVYAVIGAASAVGGLTRMTVSLVVIIFELTGAVELVLQIMMAVMISKFTADYFSTDGIYEAWIHFRGYPYLSPKEDFQPEGVTASQVMVKELVSLTAQGWTLDSLEEVVQKYEFNGFPIVSDFKNNLLLGYVPSNELRFALAQARLRPEYEGSTRCEFFTSRPASQDHQLAKSRKQEKSTGSSDHQKRDRDPVTALSSAKDSHSESRSTFDQLSGENNDVSARSSGPPGYGFIDLNKWVDEAPLTLEPETPIEIVLQFFQRLGLRNMLFTSHGSLAGILTIKDLHKYIRAPYYSSVDRAHNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KND8</accession>
    <name>E3KND8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11103</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EFP85774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    </dbReference>
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    <dbReference type="KEGG" id="pgr:PGTG_11103"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11103"/>
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    <dbReference type="InParanoid" id="E3KND8"/>
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        <location>
            <begin position="48"/>
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    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="210" mass="24025" checksum="60E97EFBD549CBCF" modified="2011-01-11" version="1">MDRSKNSGGRISWWLPEARQFSYFVVERNVWDSIKGWKDVWVKADGDRGPKGVGQSRAVGGGGGSIKRVVRRGGRGTESRKAAFQLPTNWRSQTRRAQLWYMEYSIHDELSSRLMERCMQVSILNLCRKLSEKREKAITISSINQPINQSEAGWKTKWTMWATIELRCGDGEGGQTLTEDAYRESSRDEGSEVNRSWCGYSKGEQCQLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNM1</accession>
    <name>E3KNM1_PUCGT</name>
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        </submittedName>
    </protein>
    <gene>
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
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            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178297">
        <property type="protein sequence ID" value="EFP85896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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    <dbReference type="AlphaFoldDB" id="E3KNM1"/>
    <dbReference type="GeneID" id="10544478"/>
    <dbReference type="KEGG" id="pgr:PGTG_11652"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11652"/>
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    <dbReference type="InParanoid" id="E3KNM1"/>
    <dbReference type="OrthoDB" id="10371054at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
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    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="12"/>
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        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="109" mass="11727" checksum="69723E8810366E68" modified="2011-01-11" version="1">MLKSYTPGALLAKRDRGTDKTRPIRGTNEISSEESVSRLRGPRVEHPPAGSASVHCRSLTPCALLFGAAADSLSAALLASLSAPPDDLGMDPWNDMWSPRKTVDRQELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNU3</accession>
    <name>E3KNU3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11724</name>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
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            <taxon>Puccinia</taxon>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178297">
        <property type="protein sequence ID" value="EFP85968.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003330339.2"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11724"/>
    <dbReference type="HOGENOM" id="CLU_2027887_0_0_1"/>
    <dbReference type="InParanoid" id="E3KNU3"/>
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    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="69"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85968.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="122" mass="14143" checksum="9363BDA517A99563" modified="2012-04-18" version="2">MSATRDPALHGIKFRSRSMRITGGRRVSLRRCNSLGHLRKPFVQWKCGTRLNRNRNDRLTSPHPFLRKIFWLLVGEACAFYILLYARRRVIISGELVILDVPLVQYNFLLGKLAPVVAARRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQZ0</accession>
    <name>E3KQZ0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13097</name>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178302">
        <property type="protein sequence ID" value="EFP86715.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331134.2">
        <property type="nucleotide sequence ID" value="XM_003331086.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQZ0"/>
    <dbReference type="GeneID" id="10541144"/>
    <dbReference type="KEGG" id="pgr:PGTG_13097"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13097"/>
    <dbReference type="HOGENOM" id="CLU_1256591_0_0_1"/>
    <dbReference type="InParanoid" id="E3KQZ0"/>
    <dbReference type="OrthoDB" id="2497193at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="132"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86715.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="220" mass="25020" checksum="E7A6F3EB3513121E" modified="2012-04-18" version="2">MTPKSFMLHFLHSDNSDLAYRRRYWAESAVDSTVVLVKAIRDKMNSSAPGQQAWADFIQEEAIKILAGQVPPKGNHPHGAYQSSTSVTKGYFSQEEKDKREALMTSTHMPFLYNILLAMLQQQRKHITSSEKDEDDSEDDFLTDDKDDDMPVDADVMAYAKKTSGDARLHFRYTTGAIERSPDVPKTLAKILRPSNRWDHEGTSGFHRRWCNSHPTGATR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3KRT7</accession>
    <name>E3KRT7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12753</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178303">
        <property type="protein sequence ID" value="EFP87012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331431.1">
        <property type="nucleotide sequence ID" value="XM_003331383.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KRT7"/>
    <dbReference type="GeneID" id="10542088"/>
    <dbReference type="KEGG" id="pgr:PGTG_12753"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12753"/>
    <dbReference type="HOGENOM" id="CLU_065206_0_0_1"/>
    <dbReference type="InParanoid" id="E3KRT7"/>
    <dbReference type="OMA" id="SCAAQMN"/>
    <dbReference type="OrthoDB" id="2516498at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="9"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="45"/>
            <end position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="224" mass="25562" checksum="03B4813DC4C708B6" modified="2011-01-11" version="1">MGALFNAALNGSNDTQQAETTIKQPNKGKQPQKSEQYHPVPSTSHHSHHLPSNHHRSNVNVFSRPQYDWHPAPHQAPSAAEKEMQEFQAAKLRLEQAKLISQTFSHINNRWKTKKTLRADGTYFFQWTRELREIGTSHLSDPEFFFADCDNSTFEKIGRLVIFAGVHQDLVPDIQPLKKTSNIFNLLKKKFLANSRAAQMNLWRRLSGITIKHSEPSTGVVSQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>E3KS90</accession>
    <name>E3KS90_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">TOG domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_13384</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
            <location evidence="1">Spindle</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CLASP family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EFP87165.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331584.2">
        <property type="nucleotide sequence ID" value="XM_003331536.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KS90"/>
    <dbReference type="STRING" id="418459.E3KS90"/>
    <dbReference type="GeneID" id="10537395"/>
    <dbReference type="KEGG" id="pgr:PGTG_13384"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13384"/>
    <dbReference type="eggNOG" id="ENOG502QT5T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_312413_0_0_1"/>
    <dbReference type="InParanoid" id="E3KS90"/>
    <dbReference type="OrthoDB" id="46159at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005881">
        <property type="term" value="C:cytoplasmic microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005815">
        <property type="term" value="C:microtubule organizing center"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072686">
        <property type="term" value="C:mitotic spindle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990023">
        <property type="term" value="C:mitotic spindle midzone"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005876">
        <property type="term" value="C:spindle microtubule"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008017">
        <property type="term" value="F:microtubule binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090307">
        <property type="term" value="P:mitotic spindle assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031110">
        <property type="term" value="P:regulation of microtubule polymerization or depolymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902903">
        <property type="term" value="P:regulation of supramolecular fiber organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.10.10">
        <property type="entry name" value="Leucine-rich Repeat Variant"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011989">
        <property type="entry name" value="ARM-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024395">
        <property type="entry name" value="CLASP_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021133">
        <property type="entry name" value="HEAT_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034085">
        <property type="entry name" value="TOG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567">
        <property type="entry name" value="CLASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21567:SF9">
        <property type="entry name" value="CLIP-ASSOCIATING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12348">
        <property type="entry name" value="CLASP_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01349">
        <property type="entry name" value="TOG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50077">
        <property type="entry name" value="HEAT_REPEAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="5" id="KW-0493">Microtubule</keyword>
    <keyword evidence="7" id="KW-0498">Mitosis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="TOG" evidence="11">
        <location>
            <begin position="2"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="repeat" description="HEAT" evidence="9">
        <location>
            <begin position="184"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="domain" description="TOG" evidence="11">
        <location>
            <begin position="316"/>
            <end position="554"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="245"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="532"/>
            <end position="682"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="246"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="280"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="564"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="670"/>
            <end position="681"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP87165.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1104" mass="120212" checksum="E383D730288D69F1" modified="2012-04-18" version="2">MSFATIQALEVVITGSDNEKKVLRLNTLRDELETLESPPDLQPLLTSLKSSLDSSNGHLSYAALACLPPLARTLASPAHQSALKQLVFSLYSPASSSVASFLGDAKQRTRETARAALFGAASAACEAHVISQTSPRTDDVLQEIERIVKEQGFASKIARARVQTLYYLSEIRSRYPALIPFKTYLPSLVSLLEDSDVSVRTAASECVTTVFSDPSLPASARGDLKNELAKQGTRRSTVDSILAKVFTTSNPPGNNTTAVGSGADDPPLTQTDNTVRDQAVDSSSNPLANSQTGPPGSEPPPPSGIINPVFVSGPRELESIFAKMLSPWEGKETEHNWQAREANMNTLRGMIKTNVHMQYTADFIAGLKSISEGLLKSLASLRTTMAVGACNAFVELSVLGPALDPLVDIILPPLLRMAAQTKKIVFQASQAAVTALIKSTYHPRVLQYLSACVNEKTVQARIAGVSRIKEFLDFHGQRSKSQIESGGGLGIIEKVLRRSLADASPVVRETAREVFWNCSAIWPQMTSPLIESLDGPTRKQLEKASTGRRAVSSASFKTEKEPSSRISVRSLIKSTRSAHRAAETPNVAKAAPISPRQVANSLGGIESPAIKSTGATSGSGLAHRTPERQNTAQSPRRNDLKRSVSARDPPTGDRSKSSISSPPLGLAISKTMSPHKTSPASPISRIAVRSASTGKNFSPPNQRLQSRKVLSAENIVEDAMKAQAEQAESAAHRLLELTEDENELQPLVPLGPGGVLLPATTRADHVRLTQLAQSNIPKQFQATTNKETMWKQFEDSPRNHLHIAVEEVPKKEIAHRGNKNLWWHRQAELSMCAGRQDASPSDILNLIAQIEEHPPTSQDRQIYLDLSQACYEHRCSIGKVDSTGTIIKSEEKSQMEKNMEFWMDQNLFDRLFEALKEKLKQNVSVEVKDAQLVLLRALILYETMLMGGKETELCELLLLVASEGVSNLIIAVEALGSLWAETSDPVYGLSCLRSCVEIVSQRNLINQASSNQPGSENWMGLAMSCLGGFFCRLPAEIVEEELPKASELIKRALNHRQAEIRMSAVMSLVSANQVIKNDREIFQVLGNLTTAQEALITYYLTPSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3KTB5</accession>
    <name>E3KTB5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GCF C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_13911</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178307">
        <property type="protein sequence ID" value="EFP87540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331959.1">
        <property type="nucleotide sequence ID" value="XM_003331911.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KTB5"/>
    <dbReference type="STRING" id="418459.E3KTB5"/>
    <dbReference type="GeneID" id="10538439"/>
    <dbReference type="KEGG" id="pgr:PGTG_13911"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13911"/>
    <dbReference type="eggNOG" id="KOG2136">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020074_0_0_1"/>
    <dbReference type="InParanoid" id="E3KTB5"/>
    <dbReference type="OMA" id="MKNICLW"/>
    <dbReference type="OrthoDB" id="429427at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071008">
        <property type="term" value="C:U2-type post-mRNA release spliceosomal complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000390">
        <property type="term" value="P:spliceosomal complex disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012890">
        <property type="entry name" value="GCFC2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028211">
        <property type="entry name" value="Ntr2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12214">
        <property type="entry name" value="GC-RICH SEQUENCE DNA-BINDING FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12214:SF0">
        <property type="entry name" value="LD29489P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF15458">
        <property type="entry name" value="NTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0539">Nucleus</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="484"/>
            <end position="572"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="34"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="132"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="492"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="534"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="561"/>
            <end position="572"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP87540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="900" mass="100679" checksum="1EDAE092C79A6D82" modified="2011-01-11" version="1">MEEDSNDGTLVLPIFKKKKASSSTRNKDKASLRTSSAASSTKTTIEEDPIDQQTHEEDGNTVISRVSKKSILKSTPTKSKQSRLSLGSVTDESFDLNNSSKSSSRRKLARPGSHTSLNNFSSDERSIEGSPLSRQLSESSRPTYSTAHLNELKSLTLSSVPGRSNEYTGDDDLIRSKFTGHHALEDEDISFVSSNESVHIPTSNFINSAKERRSQARKIGLSHQSTSNLLISEPSSSEDFISLSAGPSSVLPFQATKNGESRLVREDDQFGEGDDEHAEFTGAKERVPLGEKAGLAAEASKKAGLEAMIMDAEVDLHEGNEEEMEWEAAQIRRGGIGSSAEKTTNERKAEIYKPAPIPEHTPLASLASVESGLLNLLNLLEESVAEQSAAALQFTTEEKNLADQESELRQEVSREAQRADFFEELNSFVKEIDLFFTKKWPQLEKVEQDLISILKERAELISKRRYEDLSDDLVLFKDGEVGVIRPSSTKPSSRDEESEPSKAEQESEVDELGRSRPELDISPHAPSRTSRRNDRAHRRKRRVAAASIEHTVEEDDEGFSTDDSLSPADSSDLMSASKSLYDSSRAILLDITNPVFLSPTHPGSIFDRFMSWRSKYPEEYGNAFGNLALVQAWEFWVRVEIVSGLNIWGLREWVKGEDKRGIENWEWMRGLERYEHEIQSGSQADSADPQESVIAAMISTVVIPLLLPIIKSSYDPFSTRATTKSLQLAEQVSYVVETEGNPTYDKLIKCFLDRFRSSIHDLKRLVGSPRELNDKLRSQVGVEGIQARQRFLNKSFKLFKQGIRWKQFCGKNYWIDGDQEDDLGDLRRLDQKSISSNTILPLDFKYLLVHHLLLQIICPILGISWETGGQQISEKIIAACPDNFLPDEVISMLKNGQFST</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KTF9</accession>
    <name>E3KTF9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_13955</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178307">
        <property type="protein sequence ID" value="EFP87584.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332003.2">
        <property type="nucleotide sequence ID" value="XM_003331955.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KTF9"/>
    <dbReference type="STRING" id="418459.E3KTF9"/>
    <dbReference type="GeneID" id="10538644"/>
    <dbReference type="KEGG" id="pgr:PGTG_13955"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13955"/>
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    <dbReference type="InParanoid" id="E3KTF9"/>
    <dbReference type="OrthoDB" id="2507141at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP87584.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="225" mass="25172" checksum="2C221E7F438B2ADB" modified="2012-04-18" version="2">MDLAGLSASKSYGTQKCMKDNIKEDLNKRYYEFQCNITKLAIQNPVGDHCYFNHMGQSQRVRGPWSWNNFQQHDPKARKLFNEFGLDAGCPKTLQEVATKSGGTNVPHPKTVLHAPLADSLPIQLHGRVQVSKRSLEKSSTLVLTWVNKLQLDLKVFSVVVASRHPKRTIYHKGGSPISDQFLRMLAQDKDSDAAAEFHNWTAAQAIQISKGCGATAPQRTRITK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KV45</accession>
    <name>E3KV45_PUCGT</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14352</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178312">
        <property type="protein sequence ID" value="EFP88268.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332687.2">
        <property type="nucleotide sequence ID" value="XM_003332639.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KV45"/>
    <dbReference type="GeneID" id="10535659"/>
    <dbReference type="KEGG" id="pgr:PGTG_14352"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14352"/>
    <dbReference type="HOGENOM" id="CLU_1928616_0_0_1"/>
    <dbReference type="InParanoid" id="E3KV45"/>
    <dbReference type="OrthoDB" id="10290763at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="17"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88268.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="146" mass="15465" checksum="B2581564A56BA602" modified="2012-04-18" version="2">MQHSHDASQDGAHALAESNQTTNASWEGQQSSSAPPNSSANSTPVAMELEATEKVSKKIEHMTAQLDRILTNIKDVDTQLSRLAKSIEFGFGMGGNTAPLEGITAPLEGITTRLEGININEEALGISNQSANQVGQHANQVDQGNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVN3</accession>
    <name>E3KVN3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14457</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178313">
        <property type="protein sequence ID" value="EFP88373.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332792.2">
        <property type="nucleotide sequence ID" value="XM_003332744.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KVN3"/>
    <dbReference type="GeneID" id="10535846"/>
    <dbReference type="KEGG" id="pgr:PGTG_14457"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14457"/>
    <dbReference type="HOGENOM" id="CLU_066281_0_0_1"/>
    <dbReference type="InParanoid" id="E3KVN3"/>
    <dbReference type="OrthoDB" id="10300782at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="113"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="181"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="205"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="228"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="239"/>
            <end position="257"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88373.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="367" mass="40011" checksum="175393510B54935F" modified="2012-04-18" version="2">MSSLRPCGRIKQAHLDPFSSLFLESIGHGSLSFKLILSMPEPDHMAMAVDSPTRDDVSTTFTPSDATPILNHPTHAYNARRESPIEADEALAPRSPFESVATPNTAITAVRPNRTASNGARLEENAVIDPPREATDHTNPTHQASHAYPVRQATQMEEDEVPSYPPPPYVHAVRFALTSPYTTPATAGPPRSIVDPGPRRVYSTPRHTGSASPDSPTRPNVRRRLFVTSPAPTRAAPPGGSPAPPTDPPTTPHPSPAGDPFDMDARFSAQFLAMSFYNTLLSVHDVLGDLADDHPHFLRHEIMRSATIIAEDWFNYHRIHLPAQFAEPHSMGHLVAGSVRFTVRCLNALILECPDLYPSIPPLSPTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVR1</accession>
    <name>E3KVR1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_14485</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178313">
        <property type="protein sequence ID" value="EFP88401.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332820.2">
        <property type="nucleotide sequence ID" value="XM_003332772.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KVR1"/>
    <dbReference type="GeneID" id="10535874"/>
    <dbReference type="KEGG" id="pgr:PGTG_14485"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14485"/>
    <dbReference type="HOGENOM" id="CLU_072137_0_0_1"/>
    <dbReference type="InParanoid" id="E3KVR1"/>
    <dbReference type="OrthoDB" id="2515251at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP88401.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="331" mass="36967" checksum="88FFE6A97F3120D9" modified="2012-04-18" version="2">MSNPPKKKRLAPVPIIWTVSPEGISSNIGQRNKDASKTYRALDQDALDLYKGNDDDEWIDADSTEDPNIEERSQFDLRRQSTKKLLANLKELSDGLGLEGFLVIADQDHRQPYFFQGGSFLLDLFLRGLIDKGDAISRFAIWTAGTKSRKNPAVNLPAPVVSTVCKVFEVKNSPGSNLVQTINEKSSTNPSLDVSKDNTPEFLDSDKQYKGRPGNRYVCQGSLTENHTYITEKLKEIYSKAIGITKSTTHWPGTNTQYKLKKKDLMLSIADNPVQLSATHFSQTIKKISLPDTWVVLRGLKNNWISLVPFVEPENPAPINIDEANSTNSNN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="76" xmlns="https://uniprot.org/uniprot">
    <accession>E3KWQ9</accession>
    <name>E3KWQ9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Serine protease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_14692</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the peptidase S8 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178316">
        <property type="protein sequence ID" value="EFP88726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333145.1">
        <property type="nucleotide sequence ID" value="XM_003333097.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KWQ9"/>
    <dbReference type="FunCoup" id="E3KWQ9">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KWQ9"/>
    <dbReference type="GeneID" id="10541677"/>
    <dbReference type="KEGG" id="pgr:PGTG_14692"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14692"/>
    <dbReference type="eggNOG" id="KOG1153">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011263_1_4_1"/>
    <dbReference type="InParanoid" id="E3KWQ9"/>
    <dbReference type="OMA" id="RHPDVDY"/>
    <dbReference type="OrthoDB" id="206201at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005615">
        <property type="term" value="C:extracellular space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04077">
        <property type="entry name" value="Peptidases_S8_PCSK9_ProteinaseK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.80:FF:000015">
        <property type="entry name" value="Probable PRB1-protease B, vacuolar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.200:FF:000007">
        <property type="entry name" value="Subtilisin-like serine protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.80">
        <property type="entry name" value="Peptidase S8 propeptide/proteinase inhibitor I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.200">
        <property type="entry name" value="Peptidase S8/S53 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034193">
        <property type="entry name" value="PCSK9_ProteinaseK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000209">
        <property type="entry name" value="Peptidase_S8/S53_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036852">
        <property type="entry name" value="Peptidase_S8/S53_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023827">
        <property type="entry name" value="Peptidase_S8_Asp-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022398">
        <property type="entry name" value="Peptidase_S8_His-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023828">
        <property type="entry name" value="Peptidase_S8_Ser-AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050131">
        <property type="entry name" value="Peptidase_S8_subtilisin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015500">
        <property type="entry name" value="Peptidase_S8_subtilisin-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010259">
        <property type="entry name" value="S8pro/Inhibitor_I9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037045">
        <property type="entry name" value="S8pro/Inhibitor_I9_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806:SF11">
        <property type="entry name" value="CEREVISIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43806">
        <property type="entry name" value="PEPTIDASE S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05922">
        <property type="entry name" value="Inhibitor_I9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00082">
        <property type="entry name" value="Peptidase_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00723">
        <property type="entry name" value="SUBTILISIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54897">
        <property type="entry name" value="Protease propeptides/inhibitors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52743">
        <property type="entry name" value="Subtilisin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51892">
        <property type="entry name" value="SUBTILASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00136">
        <property type="entry name" value="SUBTILASE_ASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00137">
        <property type="entry name" value="SUBTILASE_HIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00138">
        <property type="entry name" value="SUBTILASE_SER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5 6" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0720">Serine protease</keyword>
    <keyword evidence="8" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="8">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173797" description="Serine protease" evidence="8">
        <location>
            <begin position="21"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="domain" description="Inhibitor I9" evidence="10">
        <location>
            <begin position="65"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S8/S53" evidence="9">
        <location>
            <begin position="219"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="307"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="222"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="253"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="5">
        <location>
            <position position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP88726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="551" mass="59035" checksum="18D7C61DEB80157A" modified="2011-01-11" version="1">MHRPSMFGFIATALAGSVLAVPFNDERDSHLAPLITPTAPPVGGPLSHLSIQAGRSPSNYLIKDNYIVVFKKGTPELHSRQHKDKVVQLWKQDQQQRLQADSYGPADLFSGIKHHFDIGLDSFKGYSIHVPEALISIVRSFPEVDYVERDSRVWINDQFENPTTIPVAENPPHPVSEPIDIEYGAPWGLARISHRHKLKFSTFNVYPFEDPAGQGVDAYVIDTGVNIEHEDLEGRARWGKTIPDDPDQDLNGHGSHVAGTIAGKTYGVAKNATIIAVKVLGAGGSGTMSDVVAGVVWAAEAAATKARDEAAKKDSKHKGSVANMSLGGGNSPSLDQAVNAAVDTGLHFAVAAGNDNRDACTFSPAAAQKPITVGASTIQDERAYFSNHGKCVDIFAPGLNIKSIWNTGKRSVNTISGTSMASPHIAGLAAYFLSIYPEKLDLASLGVYDDEEEEFVASNGEAYDLGQYFSWNTVSSQGQLAFGKFKEAVGYAGPKKSDDGPQSLSTKMLKKAMIKMSTTGVLSKLPGHTCNYLAYNNATVAPSLSLAELGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KXF0</accession>
    <name>E3KXF0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Tet-like 2OG-Fe(II) oxygenase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_15226</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178318">
        <property type="protein sequence ID" value="EFP89023.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333442.2">
        <property type="nucleotide sequence ID" value="XM_003333394.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KXF0"/>
    <dbReference type="STRING" id="418459.E3KXF0"/>
    <dbReference type="GeneID" id="10542466"/>
    <dbReference type="KEGG" id="pgr:PGTG_15226"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15226"/>
    <dbReference type="HOGENOM" id="CLU_026798_3_1_1"/>
    <dbReference type="InParanoid" id="E3KXF0"/>
    <dbReference type="OrthoDB" id="2505311at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046798">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20515">
        <property type="entry name" value="2OG-FeII_Oxy_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tet-like 2OG-Fe(II) oxygenase" evidence="2">
        <location>
            <begin position="144"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="22"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP89023.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="311" mass="35467" checksum="F355BAF78D596E40" modified="2012-04-18" version="2">MALPRQRGVNASLTRSLDGQVDSDSSPTDISSSSSESEQEEEGCNLFPEITAETKRAKIKRKLARKHFKKAGGPLPPKKKISNWLPTPEEIATASSIVGDPTKFRLYKHGHVCIFDKQLTTEKEKQIVANITFTDLKTISDQLKRDDLNFLVAFLEKSKKFVNSVGSKSRSCGGYMWAIGWRKSMTKFEIIRRYVNKAAIKKNQEQFNQHVKDSDQASLILWDLFYPIGNRALEANQQFMAEYNLPSLSDPELPSETSKGHNKFFSTNLTFTSDGFFNHPHKDKKDDKRLPFAFFLSLPTFKSKEYTHMEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>E3KY39</accession>
    <name>E3KY39_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">FUN14 domain-containing protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_15081</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FUN14 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178320">
        <property type="protein sequence ID" value="EFP89240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333659.1">
        <property type="nucleotide sequence ID" value="XM_003333611.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KY39"/>
    <dbReference type="STRING" id="418459.E3KY39"/>
    <dbReference type="GeneID" id="10546877"/>
    <dbReference type="KEGG" id="pgr:PGTG_15081"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15081"/>
    <dbReference type="eggNOG" id="ENOG502S4WM">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_095425_0_0_1"/>
    <dbReference type="InParanoid" id="E3KY39"/>
    <dbReference type="OMA" id="WTRMSSS"/>
    <dbReference type="OrthoDB" id="163794at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007014">
        <property type="entry name" value="FUN14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21346">
        <property type="entry name" value="FUN14 DOMAIN CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21346:SF10">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04930">
        <property type="entry name" value="FUN14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP89240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="231" mass="26077" checksum="0B84771DE84D5F53" modified="2011-01-11" version="1">MLAFPIIAPQICSLRSHLITQTPKLTRSSITNISRVSRQPVWSDSNCNRFLQSNKLRFQNDLKSYPTTRASIPRQSFSRTKTSVGILLAITGYQLTKPAIQLDAALPPPKFDNQPQQPIELEPEEFSSLLSVRKLSFGTLTGICAGVFVKKGLNFLAFLFGGGFVLLQYLHSSSLIKINWKTWANRYESKFWSAKEPPTKSIVARFLDFLTSDFQYRSTFTVGFFLGLRIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3KYT1</accession>
    <name>E3KYT1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Arrestin-like N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_15319</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178322">
        <property type="protein sequence ID" value="EFP89477.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333896.2">
        <property type="nucleotide sequence ID" value="XM_003333848.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KYT1"/>
    <dbReference type="GeneID" id="10547364"/>
    <dbReference type="KEGG" id="pgr:PGTG_15319"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15319"/>
    <dbReference type="eggNOG" id="ENOG502S5ZG">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_344557_0_0_1"/>
    <dbReference type="InParanoid" id="E3KYT1"/>
    <dbReference type="OrthoDB" id="3345971at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.640">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014752">
        <property type="entry name" value="Arrestin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011021">
        <property type="entry name" value="Arrestin-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00339">
        <property type="entry name" value="Arrestin_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arrestin-like N-terminal" evidence="2">
        <location>
            <begin position="172"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="440"/>
            <end position="631"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="658"/>
            <end position="712"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="766"/>
            <end position="799"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="862"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="469"/>
            <end position="496"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="523"/>
            <end position="534"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="570"/>
            <end position="584"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="881"/>
            <end position="918"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP89477.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="950" mass="103955" checksum="DD6EA4DD2C874679" modified="2012-04-18" version="2">MAETDVSSSLPAQARPEDERISLSCATSSSLLLLATHTTLTNHHHHQDQLTGQQPASNRLTFRLSKLLSRVGMSISIDNPLIFLYPKPVSASEQSHLTNIPSRNHPHNNHNNNNHNNQTTNLLSGPQNRLRSHPQAALFPSFRPPSPQPPPSSDHLTVPPSIDELDSRSTILSGSLILKFSKPKEIRKIIISLCGQSYIGFNDRPYEYQTILAKELEIDLDAQLLSNPEQHPSPGSIHYNSQYKTYILDKGNYRFNWSFILPPDLPPYERGQFGRTTHKVVAKVKLGSGLGLGLKSVGKNLTSILFGVGGGGHEIETESYFVAISNPSGSNEDLSEDLGLNLNVTELSEQLGPYTLSASSCYFTVAGLLSFKFKLQEIPQRKKIIKIYKIVCLIKQQYRLISIKDKDSRTGGPAESWPPPQRRKVFVLDHSNVLDIKRVLPNGQPLEPSDQPQSSHLAPDCLTPAFNVSSSDSAGRASSSHRPTGTIQQTSPSQSRSRSRIRGLLTSPGRSGENSGGEHSDQSRLGNHSWNGLTSIRLGRRREEPAHSEPQPAAREPRLYSSRSADRLGNSSTPANNNSTPTDPGTAAENDHPNGTAPQTTDTGYQRPRYPSHHSVLPRPGEQSSGAASNRHHVIPRESQDGQRHPEAFEAFHLEQVSPPSVGHPHRGRQQENARAGRTARDKSRTSRSRGRKSRPEHGPNESQPNPDQEIPLVTLTDDHLSWEVEHIGRLPTDDIIRPSTIPGTQTNIHIFHTLLFEIHYSVEDDNSKTKVPDSNDPGGSHGPKKSSAAECNSSPNPITRKVLSIRKPVQISSCCCMVESMVLPPYSKFDPNAQFVQHPTPSHAARMFKCVCGMSTDVLLKTQNPKPSQPGPHGDQQPETAAASSSAASSSSAASSVTTSTLTVRPSSSSNSLSPSTDLLLSSSINHQPLTGHLNCNINNFDNCFISIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZ32</accession>
    <name>E3KZ32_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15706</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178323">
        <property type="protein sequence ID" value="EFP89557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333976.1">
        <property type="nucleotide sequence ID" value="XM_003333928.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZ32"/>
    <dbReference type="GeneID" id="10547526"/>
    <dbReference type="KEGG" id="pgr:PGTG_15706"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15706"/>
    <dbReference type="HOGENOM" id="CLU_449143_0_0_1"/>
    <dbReference type="InParanoid" id="E3KZ32"/>
    <dbReference type="OMA" id="RIAANYW"/>
    <dbReference type="OrthoDB" id="10380672at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="432"/>
            <end position="517"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="584"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="446"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="505"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="598"/>
            <end position="608"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="608" mass="67762" checksum="91AAE9D1A53C2CC3" modified="2011-01-11" version="1">MGRPPNSRNIERHPTVTYISYGDSNEFKKRNRCWLASALESLYAVYSPIWLMQPEGRKKDLFSLLMSHFNSRSTWELTKSKSIRSILTRGSNSIFEAARNLHPQSFGYGLFASQDFFMEILLDTKTNSSKVLRKLFLVSEKREFSCANHPGAAPIDNSRSTQECTAIRIWKSMFVDNEINCANLEELISQWTTNGLRGVSGLQCRLSKAQDHTRKKNRKQITAVNSCPLLQELNRLLFVDGEAPAHLYFHIDLAEILNEKDQQDFQSTISCPFKLNISGTVYTLISQGFWGGISLLGESAAKWIASIKRDNPQVKEEGLIPFIGMGLVLNSSYHAAMPIPHDNSRPASDQLNTLAPSVVVSQACVNDEIIATEKEPRNLGACSSSLKIRIRLANKNPNDTTALNAQQLCPITDTQQLVQADVKPKQRRCATIAAGSRSTGHEATLLDSSPNNGSDSLTATVAPVSTAPDPNIKTVRPKKLPASQASNKPPLPTETVPIVEPSKQHPAKRGRPRKTPAMRTSVTFKPAPLNDTDWDRIQARARANWQEHWRIRDLEEATKKMGSCNKHISMDMVVEAKEPVLMEVEDSQPQESPGKGLRSCKRRARAVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZN5</accession>
    <name>E3KZN5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Small ribosomal subunit protein uS7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_15416</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178325">
        <property type="protein sequence ID" value="EFP89760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334179.1">
        <property type="nucleotide sequence ID" value="XM_003334131.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZN5"/>
    <dbReference type="FunCoup" id="E3KZN5">
        <property type="interactions" value="141"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KZN5"/>
    <dbReference type="GeneID" id="10545411"/>
    <dbReference type="KEGG" id="pgr:PGTG_15416"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15416"/>
    <dbReference type="eggNOG" id="KOG3291">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_072226_2_0_1"/>
    <dbReference type="InParanoid" id="E3KZN5"/>
    <dbReference type="OMA" id="EVHKYAM"/>
    <dbReference type="OrthoDB" id="9972728at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005763">
        <property type="term" value="C:mitochondrial small ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="entry name" value="Ribosomal protein S7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_uS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_uS7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_uS7_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="Ribosomal protein S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS7" evidence="5">
        <location>
            <begin position="112"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="8"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="28"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="46"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP89760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="251" mass="27898" checksum="0F0CB8E7B1FB8EC3" modified="2011-01-11" version="1">MKMMITSLIRSKTRQTLPSSLQTLAGYSPHPSSSSSSSSSSPSSSFTHNSNHRTFSSTRDVRFGASSVDQDRHPRARRLPVYEPPPPFPEPGDIAGQQAYREPGISVAPLEEPLLEYMIGLLMRQGKRTIAQKHVVTLLGQLRLVTGQEPFPLLVKAVQLAAPIVVTRNEQKRIKVTTVPVPLNETQSARKAILWIVKHSERRPGKKFGDRLAMEVIAVLNGNSEVLKLKAEQHRLAYVNRANLRTRATRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZN7</accession>
    <name>E3KZN7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15418</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178325">
        <property type="protein sequence ID" value="EFP89762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334181.1">
        <property type="nucleotide sequence ID" value="XM_003334133.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZN7"/>
    <dbReference type="GeneID" id="10545413"/>
    <dbReference type="KEGG" id="pgr:PGTG_15418"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15418"/>
    <dbReference type="HOGENOM" id="CLU_860895_0_0_1"/>
    <dbReference type="InParanoid" id="E3KZN7"/>
    <dbReference type="OrthoDB" id="2504288at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="19"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="96"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="53"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="148"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="215"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="323" mass="35653" checksum="D349E6B3191114D4" modified="2011-01-11" version="1">MANERNSYRDLITWGSTLDYEPSRSRAGEAAVPEGQDEPVARTRPRDRRDQWQRQGLGLSLSLSPDRKALETSLQRTSGVSARIFSGGLRDLPRILEVRSGGTSSSASPRRKASASKGERAKGVGLPSSSRKAREEGSAKPVASGSVRKKEAGVVRSSRETLMNAANTPLKRPASALVVDQDEDDDVLLRSSPTAKRRSRRTDESSGPPPVLVQPVQTIDLSPGSATRTPRPQNLHNPLLLEPTTSVRTIRKTPLALPIHRPHKPMSIFHSHPLLNPSKPTPTLPVNFSDWNWKSNLFHANWSLDDILEWERWNQKALESSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KZW9</accession>
    <name>E3KZW9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15806</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178326">
        <property type="protein sequence ID" value="EFP89850.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334269.2">
        <property type="nucleotide sequence ID" value="XM_003334221.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KZW9"/>
    <dbReference type="GeneID" id="10545582"/>
    <dbReference type="KEGG" id="pgr:PGTG_15806"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15806"/>
    <dbReference type="HOGENOM" id="CLU_860896_0_0_1"/>
    <dbReference type="InParanoid" id="E3KZW9"/>
    <dbReference type="OrthoDB" id="2507840at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="23"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="111"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="121"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="169"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="186"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89850.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="323" mass="35086" checksum="6EC98F3C14042057" modified="2012-04-18" version="2">MFSSNSTLSFSSTELNSSHLNHYSRAPFRPRNHPKSRSKLHHHHQHQHDHHPSWSSSLSAFSLQQQQRASGFLIDEYGNEHDPERKPWTQPSVNLVATRAAPHPPAPPRMPVCVPGAAAAAPPPPAEPPPPTPPSPHPPEPPSRTRPAAVPTGPARALKRSPTSSSTPPTTPSRLRPPPPHPPASPAGHPSNPGPRSFSSSSPTTTASSSRGSRSSWTRTLWAHLRLSSSSSPAPPSPPVSYALHQAAHPIFSLPTHPAPAKPPAHLPFLTRFHLSPASEHPHHLSAVRKRLELVKLEVRFGILRTRKRVVGAWKQQTGCKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3L0T3</accession>
    <name>E3L0T3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_15985</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178329">
        <property type="protein sequence ID" value="EFP90137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334556.1">
        <property type="nucleotide sequence ID" value="XM_003334508.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L0T3"/>
    <dbReference type="GeneID" id="10546196"/>
    <dbReference type="KEGG" id="pgr:PGTG_15985"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15985"/>
    <dbReference type="HOGENOM" id="CLU_2050823_0_0_1"/>
    <dbReference type="InParanoid" id="E3L0T3"/>
    <dbReference type="OrthoDB" id="10379754at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP90137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="120" mass="13574" checksum="C33C9A51B4C44779" modified="2011-01-11" version="1">MGCFPGSQYFLYSRIPLRPHYQSAGLTTALCLFITNTQSSSLRIAAKYQKESSSRQRCTLMSWDVFNEPSRPLTNYHVKPVEVDIESGQIWPYIPHSSQIRIYNGVPQPQLSALAKHAGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3L2C7</accession>
    <name>E3L2C7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_16740</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP90714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="KEGG" id="pgr:PGTG_16740"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16740"/>
    <dbReference type="HOGENOM" id="CLU_1907721_0_0_1"/>
    <dbReference type="InParanoid" id="E3L2C7"/>
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        <source>
            <dbReference type="EMBL" id="EFP90714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
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</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
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    <name>E3L3Z8_PUCGT</name>
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
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            <taxon>Pucciniomycetes</taxon>
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            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP91273.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003335644.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L3Z8"/>
    <dbReference type="GeneID" id="10529019"/>
    <dbReference type="KEGG" id="pgr:PGTG_17130"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17130"/>
    <dbReference type="HOGENOM" id="CLU_084549_0_0_1"/>
    <dbReference type="InParanoid" id="E3L3Z8"/>
    <dbReference type="Proteomes" id="UP000008783">
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    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173997" evidence="2">
        <location>
            <begin position="19"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="24"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="127"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="27"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP91273.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="206" mass="21309" checksum="7D27409408DF491C" modified="2012-04-18" version="2">MQFTVLAAALAVFTGVLASPAVEKAEAPATDKAAKTTDAPENWPMHRGSGSFNSNTGFNSGNNWYSGQYGNGQFGNGQYGNGQYGNGQFGNGQFGNGQFGNSQYGNGQFGNGQFGNGQFGNGQFGSGQYGSGQYGNGQYGNGQFGNGQYGYGQFSNGQPGYGQYGYGQSGNGQSGNSGWGGYGGFSSYQQPQGSNNYYGSYQFGSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3L514</accession>
    <name>E3L514_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Ribosome maturation protein SDO1/SBDS N-terminal domain-containing protein</fullName>
        </recommendedName>
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    <organism evidence="3 4">
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        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
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    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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    <dbReference type="InterPro" id="IPR019783">
        <property type="entry name" value="SDO1/SBDS_N"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="FYSH domain"/>
        <property type="match status" value="1"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
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            <end position="126"/>
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    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP91639.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="126" mass="13404" checksum="7546C1DC31172911" modified="2011-01-11" version="1">MSPQITKVIYKPDSTSTDEFICIIADAGAYKKWLGGDKTVPLVEIVDSFDVFHTGQGAQGLLIRPSKQELENTFGSSKDDEVVTIVLEKGRLEVSDGHPLKFASKNDARGGNTGARVAGASGHGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3L5P1</accession>
    <name>E3L5P1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_18060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178353">
        <property type="protein sequence ID" value="EFP91866.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336285.2">
        <property type="nucleotide sequence ID" value="XM_003336237.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L5P1"/>
    <dbReference type="GeneID" id="10531812"/>
    <dbReference type="KEGG" id="pgr:PGTG_18060"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18060"/>
    <dbReference type="HOGENOM" id="CLU_1661631_0_0_1"/>
    <dbReference type="InParanoid" id="E3L5P1"/>
    <dbReference type="OrthoDB" id="2500291at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP91866.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="182" mass="19528" checksum="1F394055ECBE096C" modified="2012-04-18" version="2">MQMICSGEKPVGYLVMSGKSKSKMYSWNGRSFSSLTGNCTCASLFSRDSNAVRILACKEKPKIDLLAGKVLPFCWDGDSDSQTDSSASTCPKLHKQMYDDEETVQAKLCSGGAEVGILYASGDVLPASGKSDETIQECQCTPDRQSKDLMSYLSCANTVPKLAGAQDPKKIVCVNPLVACQT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3L5Y7</accession>
    <name>E3L5Y7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RING-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">PGTG_18413</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="5" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178355">
        <property type="protein sequence ID" value="EFP91962.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336381.2">
        <property type="nucleotide sequence ID" value="XM_003336333.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L5Y7"/>
    <dbReference type="GeneID" id="10531914"/>
    <dbReference type="KEGG" id="pgr:PGTG_18413"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18413"/>
    <dbReference type="HOGENOM" id="CLU_070137_0_0_1"/>
    <dbReference type="InParanoid" id="E3L5Y7"/>
    <dbReference type="OrthoDB" id="6270329at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061630">
        <property type="term" value="F:ubiquitin protein ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16448">
        <property type="entry name" value="RING-H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051826">
        <property type="entry name" value="E3_ubiquitin-ligase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001841">
        <property type="entry name" value="Znf_RING"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765:SF416">
        <property type="entry name" value="E3 UBIQUITIN-PROTEIN LIGASE GODZILLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22765">
        <property type="entry name" value="RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13639">
        <property type="entry name" value="zf-RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00184">
        <property type="entry name" value="RING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50089">
        <property type="entry name" value="ZF_RING_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RING-type" evidence="3">
        <location>
            <begin position="268"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="1"/>
            <end position="10"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="47"/>
            <end position="59"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="EFP91962.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="341" mass="38598" checksum="39F527ED4D9D7F80" modified="2012-04-18" version="2">MDPNTNNPLQLGSAEYPQLQGPGGSPDLNAPHPPLSPLNESFIDNAGPRDDGQGEDERLPGPPDASSAPSPTEFDPDEPLETLSVFTLIMHPDTFDILNHDHVPQEIWQDVMKFVTQLYARDAHLLPPRRRIRIPDFSDLTQIGHVLQTLEPDDRTALQHFFSDIQSTIRARALVISDPHDLANTDLRPDFAETERMRLALSRIRPEHLQDYTNFLDNLMQEYAELNSHQVESLPGRLMLDHFLDVHSVISTSFTAPNITDHNNIPECTICLEGYSPDHLITRLPCHRDHYFHLSCLESWLPDNFNCPLCRTPITIHIRFYYHAPLPAPLPVAGQDDSDAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="84" xmlns="https://uniprot.org/uniprot">
    <accession>E3L6I3</accession>
    <name>E3L6I3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Mitogen-activated protein kinase</fullName>
            <ecNumber evidence="9">2.7.11.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_17888</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Responds to activation by environmental stress by phosphorylating downstream targets.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.24"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">Activated by threonine and tyrosine phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. MAP kinase subfamily.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.24" evidence="9"/>
    <dbReference type="EMBL" id="DS178359">
        <property type="protein sequence ID" value="EFP92158.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336577.2">
        <property type="nucleotide sequence ID" value="XM_003336529.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L6I3"/>
    <dbReference type="SMR" id="E3L6I3"/>
    <dbReference type="STRING" id="418459.E3L6I3"/>
    <dbReference type="GeneID" id="10537485"/>
    <dbReference type="KEGG" id="pgr:PGTG_17888"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17888"/>
    <dbReference type="eggNOG" id="KOG0660">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000288_181_1_1"/>
    <dbReference type="InParanoid" id="E3L6I3"/>
    <dbReference type="OrthoDB" id="192887at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004707">
        <property type="term" value="F:MAP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000750">
        <property type="term" value="P:pheromone-dependent signal transduction involved in conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.510.10:FF:000040">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000073">
        <property type="entry name" value="Mitogen-activated protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003527">
        <property type="entry name" value="MAP_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050117">
        <property type="entry name" value="MAPK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24055">
        <property type="entry name" value="MITOGEN-ACTIVATED PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01351">
        <property type="entry name" value="MAPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 8" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="2 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="10">
        <location>
            <begin position="11"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00055111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP92158.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="271" mass="31444" checksum="3E7D6AB16D442CAE" modified="2012-04-18" version="2">MDRNFRVGPDYSIIDVVGEGAYGVVCSAVHEPSKQKVAIKKITPFDHSMFCLRTLREIKLIRWFSHENIISILDIVKPPSLEEFTEVYLIQELMETDMHRVIRTQDLSDDHCQYFIYQTLRGLKALHSAAVLHRDLKPSNLLLNSNCDLKICDFGLARSAFMGEQEATGFMTEYVATRWYRAPEIMLTFKEYTKAIDVWSVGCILAEMLNGKPLFPGRDYHHQLTLILDILGTPSLDDFYAISSHRSRDYIRALHSRRRNRLDNCIRMRQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="66" xmlns="https://uniprot.org/uniprot">
    <accession>E3L7G9</accession>
    <name>E3L7G9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Aconitate hydratase, mitochondrial</fullName>
            <shortName evidence="13">Aconitase</shortName>
            <ecNumber evidence="13">4.2.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PGTG_18319</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>citrate = D-threo-isocitrate</text>
            <dbReference type="Rhea" id="RHEA:10336"/>
            <dbReference type="ChEBI" id="CHEBI:15562"/>
            <dbReference type="ChEBI" id="CHEBI:16947"/>
            <dbReference type="EC" id="4.2.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="13">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate from oxaloacetate: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the aconitase/IPM isomerase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.-" evidence="13"/>
    <dbReference type="EMBL" id="DS178365">
        <property type="protein sequence ID" value="EFP92494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336913.1">
        <property type="nucleotide sequence ID" value="XM_003336865.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L7G9"/>
    <dbReference type="FunCoup" id="E3L7G9">
        <property type="interactions" value="518"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L7G9"/>
    <dbReference type="GeneID" id="10538489"/>
    <dbReference type="KEGG" id="pgr:PGTG_18319"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18319"/>
    <dbReference type="eggNOG" id="KOG0453">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006714_2_2_1"/>
    <dbReference type="InParanoid" id="E3L7G9"/>
    <dbReference type="OMA" id="KKQGMLG"/>
    <dbReference type="OrthoDB" id="2224430at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042645">
        <property type="term" value="C:mitochondrial nucleoid"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003994">
        <property type="term" value="F:aconitate hydratase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003690">
        <property type="term" value="F:double-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01584">
        <property type="entry name" value="AcnA_Mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.19.10:FF:000002">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000003">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.499.10:FF:000004">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1060.10:FF:000001">
        <property type="entry name" value="Aconitate hydratase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1060.10">
        <property type="entry name" value="Aconitase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050926">
        <property type="entry name" value="Aconitase/IPM_isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015932">
        <property type="entry name" value="Aconitase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006248">
        <property type="entry name" value="Aconitase_mito-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01340">
        <property type="entry name" value="aconitase_mito"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005558">
        <property type="entry name" value="PRK07229.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43160">
        <property type="entry name" value="ACONITATE HYDRATASE B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43160:SF3">
        <property type="entry name" value="ACONITATE HYDRATASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 13" id="KW-0408">Iron</keyword>
    <keyword evidence="9 13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="11 13" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 13" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="15">
        <location>
            <begin position="69"/>
            <end position="505"/>
        </location>
    </feature>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="16">
        <location>
            <begin position="585"/>
            <end position="713"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="518"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="14">
        <location>
            <begin position="518"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU362107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EFP92494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="782" mass="85113" checksum="0FC5DF0FD37AB86E" modified="2011-01-11" version="1">MITCQVRRLSSKPVLQSITRSYASHASKIGDTKVSMSNLEKEKYINYQRIEDNLQVVRRRLNRPLTLSEKVLYGHLDNPEDADIRRGASYLKLRPDRVACQDATAQMALLQFMTAGLPEVAVPTTVHCDHLIEAQIGGTKDLARAVATNKEVYDFLASCSAKYGIGFWRPGSGIIHQIIFENYAFPGGLMIGTDSHTPNAGGLGMIAVGVGGADAVDVMANLPWELKCPKVIGVKLTGQISGWTSPKDVILKVAGILTVKGGTGAIVEYFGPGVESLSATGMGTICNMGAEIGATTSMFPYNRRMADYLNATKRGNIAKYADQFKHNLQSDEGAQYDEMIEINLNELEPHINGPYTPDLATPLSTFAKTVEEKGWPKELKVALIGSCTNSSYEDMSRAASIAKEAHDHGLNVKSKFTITPGSEQIRATIARDGQMEVLENVGGLVLANACGPCIGQWDRQDVKKGDVNSIITSYNRNFTGRNDANPSTHAFVASPDLVTAMAFAGDLTFNPMKDTLKGKDGKEFRFSDPSGKELPPRGYDAGENTYQSPPADRQSINVAVSPSSDRLQVLKPFKAWDGKDPANLPVLIKASGKCTTDHISAGGPWLKYRGHLENISQNMLIGAINEANGKPNEVLNQETGKWGGVPEVAAYYRDNGIPWCVIGDQNYGEGSSREHAALEPRFMGGVAVITRSFARIHETNLKKQGMLPLNFENPADYEKVKPDDRIDLKGITELKEGSKVTMIAKHKDGSADQIPLTHTFNQGQIEWFKAGSALNLMGKMKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3LAN9</accession>
    <name>E3LAN9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Osmotin thaumatin-like protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_19613</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178400">
        <property type="protein sequence ID" value="EFP93614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338033.1">
        <property type="nucleotide sequence ID" value="XM_003337985.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LAN9"/>
    <dbReference type="STRING" id="418459.E3LAN9"/>
    <dbReference type="GeneID" id="10534822"/>
    <dbReference type="KEGG" id="pgr:PGTG_19613"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19613"/>
    <dbReference type="eggNOG" id="ENOG502S07P">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_043181_5_1_1"/>
    <dbReference type="InParanoid" id="E3LAN9"/>
    <dbReference type="OMA" id="TESARIW"/>
    <dbReference type="OrthoDB" id="2499003at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.110.10">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037176">
        <property type="entry name" value="Osmotin/thaumatin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001938">
        <property type="entry name" value="Thaumatin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31013">
        <property type="entry name" value="THAUMATIN FAMILY PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31013:SF2">
        <property type="entry name" value="THAUMATIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00314">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002703">
        <property type="entry name" value="Thaumatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00347">
        <property type="entry name" value="THAUMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00205">
        <property type="entry name" value="THN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49870">
        <property type="entry name" value="Osmotin, thaumatin-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51367">
        <property type="entry name" value="THAUMATIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173250" description="Osmotin thaumatin-like protein" evidence="2">
        <location>
            <begin position="21"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="80"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="disulfide bond" evidence="1">
        <location>
            <begin position="92"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR002703-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP93614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="182" mass="18894" checksum="7A695E3FEB8091CB" modified="2011-01-11" version="1">MMKCTLLLLIGSSMCGVALGYTLHFQNNCPFTVWPAVGKAPNGQPDPSVAYGAKLEPGGSSQFGVNDGEIGIRSWGRTGCDVNGANCATGACNGGLQCTDGGITSGVLLGEYGYQQFGNVISWDLSRVDGSINIDTSINNGGNLKTCRQSNCSSDQAFAQPNDFQAVTTSPVGSTFDITFCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3LAS2</accession>
    <name>E3LAS2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosducin thioredoxin-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_19596</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
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        <text evidence="1">Belongs to the phosducin family.</text>
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        <source>
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        <source>
            <dbReference type="Google" id="ProtNLM"/>
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
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        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
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    </reference>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
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</entry>
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        <recommendedName>
            <fullName evidence="2">DNA replication checkpoint mediator MRC1 domain-containing protein</fullName>
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    <gene>
        <name evidence="3" type="ORF">PGTG_19913</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
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        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003338245.2"/>
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    <dbReference type="GO" id="GO:0005730">
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        <property type="project" value="GO_Central"/>
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        <property type="entry name" value="Repl_chkpnt_MRC1_dom"/>
    </dbReference>
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        <property type="entry name" value="AGL056WP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48209:SF2">
        <property type="entry name" value="FI24008P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09444">
        <property type="entry name" value="MRC1"/>
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    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
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        <location>
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    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
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            <end position="139"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
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        </location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="454"/>
            <end position="503"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="536"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="663"/>
            <end position="677"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="712"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="906"/>
            <end position="916"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="956"/>
            <end position="985"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1082"/>
            <end position="1097"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="1104"/>
            <end position="1126"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1150"/>
            <end position="1171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1318"/>
            <end position="1342"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1345"/>
            <end position="1354"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="1359"/>
            <end position="1368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP93874.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1368" mass="150514" checksum="58236314A3DFC8DC" modified="2012-04-18" version="2">MSSLLPKPKRVYGQERTTTTEEQPVIPPTSTLEPQSSPLPLTPTEEEEEPIQSTTKNFKRIRPLQLNPQSPQPKKLKSKPTNTLEDDDDDDDHELPDIDHAFLNPAIPASPKTICEPDPIINEPDEEDEIDQLESDSEPIQEKPKTKKKKLKGLSKKEKEETQRIIANCERQKNSRLTAACRAPLSRLEDIVKKASTTTPTGAKSRSERPPYSRSHSSTHTINPLNLRLTEKNKKRDQPPTSPIKDFSDDEQDGLPTLEELTSRDRLTTKLKNKTTIPDSLENSKKKEWLANLKKNLAAAPDETIEQLDSDSDIDIIHPPTLTTSHKIDNQPAPSNQLLDPFKQRLSRGKTDSKPDKVQPHLASSRQVPSSSKTKTGSDPKSHNSKGLMDDLLAKSAVDSALLRKKKHTEFLERGGRVITRELTMTSNHNPSDIEKVDLKTILQKNKENADSNKDDDDDLDYDWDELGSMDEVESSEEEEGEERNQAEDDPDEDEVENSEEDVQMTGRALDVSDADSSSTSKPVALMLPPAVARCSMSSSSSAGFSSPSFAIPQPPPPGQISMTSKILAPDSTSTDRESSRSSKAPASEPSETGEVQPLPGSSSPVDLPGFQFEGPSHSAKKHQTIEGSPTLTGFGNVECSGIDGFSDSGVEGALDLFRQPKSKTGNNSVDNASGFSQLFDEDEGDATFTHPPPLLKPKKTRILDDDDDNARNGQEPGSKTRQAGKLLVADDDDDDDMNEIDATCVLPAVNVLPEEKERDMMMMVMGAQDTAVEEDEQPTQYVNHRGFLTQHKPNGPPDSPLMSQDVIARTPFALRKDGASWQRRGSIQREMEASPTKGQGGSSSWRVIRSASPVSAPKQPTQVMNAFTKVMDAQKEQTATTFKSPPAPNKKAPGSEGAGKMFLADEADESEDEFEGLLGRRRKGERGSGSDDENGSEGEGSLKDLVDDGEDERDEETRKGDELARRELDKKYQDEMEQRRTEHAQKIVAGKVRLKQARLHRSGDAAISESDSEDDLEIEAVKANKRAQEHAAKKRKRAIHHLADKHASFFKSYEEGTSNAIDDDDLAYLQPQEETKKTNPDEDDDDEDNEEDDDDGERYGYSEGEEKEEEEEEEEDEEDDDDELPGDILLQGLKKSKKAVDQNAGNENEGAKGEGEGTKGQKRGEKRVRLDSSPVLSSAALPALPFKITNHNTLHHHHRSMIPTKKGPATAGTNDQHEDLDDLYGASKSSKEALSERQRLLVVNELGLGAGSNARETAADQELPRRIGFAGKSATRSSAMSSSVTYFKQTNLATGTDKNPRTSSSSLMKEKNKKKKTDVGRSDKLVDHLDPQNGLHRDPSGPKKIGKLARLKAKNPSSNPPPPTTSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3LBI1</accession>
    <name>E3LBI1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19934</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178416">
        <property type="protein sequence ID" value="EFP93906.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338325.2">
        <property type="nucleotide sequence ID" value="XM_003338277.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LBI1"/>
    <dbReference type="GeneID" id="10535438"/>
    <dbReference type="KEGG" id="pgr:PGTG_19934"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19934"/>
    <dbReference type="HOGENOM" id="CLU_146198_0_0_1"/>
    <dbReference type="InParanoid" id="E3LBI1"/>
    <dbReference type="OrthoDB" id="2508582at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="70"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="79"/>
            <end position="91"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP93906.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="106" mass="11579" checksum="4228BABC97CD96FE" modified="2012-04-18" version="2">MTCAFHCPTLTEGGCVRNTGRDKKGNVNKWEVVKAFATLGYAEYYNCLGLNADLSVCAEMGKIVLPPSPQPQVMTINSEKTRKELGEDSKPFDITAGDPEECSYTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3LC26</accession>
    <name>E3LC26_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_20125</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178441">
        <property type="protein sequence ID" value="EFP94101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338520.1">
        <property type="nucleotide sequence ID" value="XM_003338472.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LC26"/>
    <dbReference type="STRING" id="418459.E3LC26"/>
    <dbReference type="GeneID" id="10535801"/>
    <dbReference type="KEGG" id="pgr:PGTG_20125"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20125"/>
    <dbReference type="HOGENOM" id="CLU_436237_0_0_1"/>
    <dbReference type="InParanoid" id="E3LC26"/>
    <dbReference type="OMA" id="GNTHESQ"/>
    <dbReference type="OrthoDB" id="2507243at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="255"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="369"/>
            <end position="420"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="508"/>
            <end position="608"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="622"/>
            <end position="644"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="20"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="88"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="135"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="194"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="229"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="317"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="547"/>
            <end position="557"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="567"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="622"/>
            <end position="632"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP94101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="644" mass="67705" checksum="6A4DBC335EA974A2" modified="2011-01-11" version="1">MAHHHSERQPLEGLPPLPSTDPTSTGTGTGSSTANQPIPPSFDPSLVNPPPPSTIGDSDSAKASKSSPSSDPANSPTNDPLSPPTDPNNPGNSGSSTSKPPDQSLGAPAPTSSSLPSSDHSKHPNDPTSPHDGASTTPSDSPLPGSPTSSSPASVASQPPQSPSEQSQNPSQTSSASLTTASAAPVPASNTSSKRPNPTSPIQSDLPSGQPIAGTVNPSTIDQATGAKSADSSASRAARPQQQNSHSDDSTGTHVGTIMGILVAAVIGALILAGILGMIKRKRRERQNQAAFHQDIFQEKNSFEPEMDRKSFGSARNPMNTEGSRSFNPRGDGIFGPRPPSIIERHQASPAIPAPMPSYQPGQVLNYPHYPGQPQQQSFNLHRPKPPTYHSGYPEESGLAYAANPPVEPSPNPRSNNTHDQKHVTLEMSHQDSPQSNRYEYHGGNQHIPMQNMTGHGTAGGLSPSTYPVTGGVVNHPGPVPNYQQQVDQLDFFGQPSPALTMVGHMTTDWNQGGSPHEKPLAPPPLSPISMMTPPAPLPSEEDRSGTPMNPNLQQSYLDCDSKVSEHLPISDSLPHPSSDDHGFQSAPISGNEDWKLASPHLSSPLGEMFKDDAYLGASIDQHHLSDTDHNKPSAHSSLKVINP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>H6QNR8</accession>
    <name>H6QNR8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_20622</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EHS62500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890817.1">
        <property type="nucleotide sequence ID" value="XM_003890768.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QNR8"/>
    <dbReference type="GeneID" id="13542537"/>
    <dbReference type="KEGG" id="pgr:PGTG_20622"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20622"/>
    <dbReference type="HOGENOM" id="CLU_1310655_0_0_1"/>
    <dbReference type="InParanoid" id="H6QNR8"/>
    <dbReference type="OrthoDB" id="2508175at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS62500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="210" mass="22709" checksum="3E6C5671BF443F3B" modified="2012-04-18" version="1">MESEPIDDLQKKKLILESGLMSLGFRIPRQLNQLASKKFSSCDFDFGVTEDSRGHSGLESVEVECDLLLRTSCFVPPSRTCARVEWAKVSVIKPQSFDSHRTPSTHHQTSFENYSTSHIFITLLNTIPSLPKNLLKSSMNFNASSFVALALSMGAFLAFASPLPRPVLPDGKKCAGLGNCNTANLGDLVNVENVATGPLGILVAPITGQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>H6QP88</accession>
    <name>H6QP88_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20781</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EHS63202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890757.1">
        <property type="nucleotide sequence ID" value="XM_003890708.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QP88"/>
    <dbReference type="GeneID" id="13541489"/>
    <dbReference type="KEGG" id="pgr:PGTG_20781"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20781"/>
    <dbReference type="HOGENOM" id="CLU_1866138_0_0_1"/>
    <dbReference type="InParanoid" id="H6QP88"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="107"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="123"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="137" mass="14669" checksum="606C338192816FD2" modified="2012-04-18" version="1">MSLVAVKSASSAYKPPRFNAHPASHENPKATPSTSKQHQPTPETSGPAKDATHTNIPNSANSTSSHHNINMAVSTRFQPPRPIKPKSKQARPSAVASGSVDGDRVTWSHSGPSQPRYTGEEGGFLKEAYHTETDESE</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPD0</accession>
    <name>H6QPD0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Secreted protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20680</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EHS63586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890647.1">
        <property type="nucleotide sequence ID" value="XM_003890598.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPD0"/>
    <dbReference type="GeneID" id="13541545"/>
    <dbReference type="KEGG" id="pgr:PGTG_20680"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20680"/>
    <dbReference type="HOGENOM" id="CLU_1886739_0_0_1"/>
    <dbReference type="InParanoid" id="H6QPD0"/>
    <dbReference type="OrthoDB" id="10281600at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003605688" description="Secreted protein" evidence="1">
        <location>
            <begin position="29"/>
            <end position="144"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="144" mass="16357" checksum="FC6A1B67FFB4FC28" modified="2012-04-18" version="1">MSRAGSFQMAHLALTLIVLISFHSGLLAVPQRCESIFAPHSGDEYTCMNPRGVRFLCGINSCLTDGLKWADVIFKHCQKYEPENTLTDHYEDIHVGEFVTVVYKKSQFLDAKKLGTNTWFRCTWSRRSPFNAHRPTCGSCRPDP</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPJ0</accession>
    <name>H6QPJ0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Oxidoreductase AflY</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20857</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EHS63875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890570.1">
        <property type="nucleotide sequence ID" value="XM_003890521.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPJ0"/>
    <dbReference type="GeneID" id="13543178"/>
    <dbReference type="KEGG" id="pgr:PGTG_20857"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20857"/>
    <dbReference type="eggNOG" id="ENOG502S69W">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
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    <dbReference type="OrthoDB" id="10004862at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044550">
        <property type="term" value="P:secondary metabolite biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="Questin_oxidase-like"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EHS63875.1"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="483" mass="54867" checksum="F8041DD5B6F9F660" modified="2012-04-18" version="1">MSSKDLSTDGGLSKLSVSHRSSFTPNTSVVINFPAENRHQTANLIADLLSDNHVKYHCFFNEKRFHNHIVHHILALYSLGADGKAIQASYDREAPTQKPIGGVDNENITAKNWTSFLGQAECYKNYLDFFWEEVKKKGAVEAVQEYIFGNPESQMLNRTFSGVLHPLIHWGYGLEFKIDAIVAEGLAITAVHGANLSNLKLSELLTKRVTNGASKGDTSNGHQKEIAPSFDFRCEETNKTFKPKTGLSAFQILHKISQETELPDPSFQTYNTREQFSMAAEHPALVPWLDKWEIEDDCDWKEILERTKELIWMVAVIYTTSYDKEKGKFALNFFLMHLVTSSLFLPAILPYLDQRLRPTLLKAFFKTAICIWVGQGRFELRISECLKEPSFIQVPDSQNPGESENPWYKVLESAAKHPDEHTTKIIRALYFNANTYGSSQPGYYSCDLKGSELLDSTLFLRASIMTLNKLEWSGEGAMDFKWY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPV6</accession>
    <name>H6QPV6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_20907</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EHS64294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890450.1">
        <property type="nucleotide sequence ID" value="XM_003890401.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPV6"/>
    <dbReference type="GeneID" id="13543008"/>
    <dbReference type="KEGG" id="pgr:PGTG_20907"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20907"/>
    <dbReference type="HOGENOM" id="CLU_3088297_0_0_1"/>
    <dbReference type="InParanoid" id="H6QPV6"/>
    <dbReference type="OrthoDB" id="10346558at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003606348" evidence="1">
        <location>
            <begin position="25"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="52" mass="5771" checksum="2E24BCB890BB8F89" modified="2012-04-18" version="1">MRVQIASIVLTALTLMVLSEGSAAAPVIKARSPNGFWDGFNRFANKPFKDWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQQ4</accession>
    <name>H6QQQ4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21234</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EHS62772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890189.1">
        <property type="nucleotide sequence ID" value="XM_003890140.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQQ4"/>
    <dbReference type="GeneID" id="13541338"/>
    <dbReference type="KEGG" id="pgr:PGTG_21234"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21234"/>
    <dbReference type="HOGENOM" id="CLU_004591_4_0_1"/>
    <dbReference type="InParanoid" id="H6QQQ4"/>
    <dbReference type="OrthoDB" id="10346569at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="14"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="18"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="51"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="72"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="244" mass="28497" checksum="7E36D145808EED6A" modified="2012-04-18" version="1">MTNRKRHCMNKRHQSLVQEHKERQMERDSRRAAALVLNQEAMDIDQTTDVAPDRNEHEKLTEDTHRDVETQTFEDPEEVPLSQFEEEDSDDEEGELQWTDMIEIAVGQINSEPEEAALAATDPLFRREEAEIAKIRPDNSAWYPFLNKEYLVGSLLIGYLHKLISRDMYHQIRIVFTLYHIRIPRWEAVRLQRAKIRSLVNHTIIEKETVFGQPVFGLDARELIKDVGTYLPIEAIFGPGEFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQV1</accession>
    <name>H6QQV1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21253</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EHS62880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890115.1">
        <property type="nucleotide sequence ID" value="XM_003890066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQV1"/>
    <dbReference type="GeneID" id="13541536"/>
    <dbReference type="KEGG" id="pgr:PGTG_21253"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21253"/>
    <dbReference type="HOGENOM" id="CLU_2723408_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQV1"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="51"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="72" mass="7971" checksum="456FD682C08F7EE8" modified="2012-04-18" version="1">MGRLIAWRQTKEDGKLSNRTIFCSISVIVDLLSKTVLAVFLKAAKTSSVTKPASTRRREVQAGLMPDRHKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQY2</accession>
    <name>H6QQY2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_21269</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EHS62919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890079.1">
        <property type="nucleotide sequence ID" value="XM_003890030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQY2"/>
    <dbReference type="GeneID" id="13540867"/>
    <dbReference type="KEGG" id="pgr:PGTG_21269"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21269"/>
    <dbReference type="HOGENOM" id="CLU_019847_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQY2"/>
    <dbReference type="OrthoDB" id="2507825at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="23"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="206"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="451"/>
            <end position="539"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="607"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="233"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="2">
        <location>
            <begin position="63"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="206"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS62919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="690" mass="75522" checksum="F29E9249FA78F5C4" modified="2012-04-18" version="1">MRHKIPTKDPQMLQPKIHHYIQVPPSEARASREGRFTPAQVTRPSDRGTCVKLDTVETGDTNPFPPHPHPTGPTAPTRRKHQPGPATNNQGQPSMDPREHTSGNLLSADGLGLHSYDPLTNSTYSSLGQDDRMHHHGLHASQTNIEVSGGHYGTPSQAIGSLTGVHRVGGAIPSGQLSYTRTAVPATPPSGILTMSAPARIDPISSSAASARLKSSSTRKQNRTRAEIDAAKAVTAAKKAEKIQRAAQRLEAQRQKQAAKEARAALKVTDTGTPTPRFVWSEDALLELLRFVKEVKEEHDDLSERTPGFIAWSPYFLNREADRDLFPLLIGIANDVILRRYRALMNVWKVVFDRLSHSGSDGLHDVLAKEHLSETLYNFMNAMHGDNAAANAYGHIELDDDLGALVDDVGPEETVDGQASVMTNTVLEDAQLVRDRRGEAGLTAAELALDDSDNDVTGQDSPLATIDGGRPPASSLFDGIPRGPTLPAPMTRPQATPAPPSIEAPVGPGVVITKPPPATPHPSRRRGRTEVAKPDDSSTQALLQMFQQSQDRQEAARVEDRRLADVRANEKESARAAAATQAAAATQAAHDRSIRDGQLKLDRDVAEKRNQALDEERRVRDEDRKEEKRQALEWRQEEGRRYEASQKLLAEERKVQDTARREQERASQLFQAAMMRMLGVQDLLPTAGKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRG1</accession>
    <name>H6QRG1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">F-BAR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_21447</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
            <location evidence="1">Cytoskeleton</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EHS63250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889894.1">
        <property type="nucleotide sequence ID" value="XM_003889845.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRG1"/>
    <dbReference type="FunCoup" id="H6QRG1">
        <property type="interactions" value="28"/>
    </dbReference>
    <dbReference type="STRING" id="418459.H6QRG1"/>
    <dbReference type="GeneID" id="13540631"/>
    <dbReference type="KEGG" id="pgr:PGTG_21447"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21447"/>
    <dbReference type="eggNOG" id="KOG2398">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_004415_0_0_1"/>
    <dbReference type="InParanoid" id="H6QRG1"/>
    <dbReference type="OrthoDB" id="19092at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120104">
        <property type="term" value="C:mitotic actomyosin contractile ring, proximal layer"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005543">
        <property type="term" value="F:phospholipid binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007010">
        <property type="term" value="P:cytoskeleton organization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.60:FF:000045">
        <property type="entry name" value="Cell division control protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031160">
        <property type="entry name" value="F_BAR_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001060">
        <property type="entry name" value="FCH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036028">
        <property type="entry name" value="SH3-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065:SF7">
        <property type="entry name" value="NOSTRIN, ISOFORM H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23065">
        <property type="entry name" value="PROLINE-SERINE-THREONINE PHOSPHATASE INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00611">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00055">
        <property type="entry name" value="FCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103657">
        <property type="entry name" value="BAR/IMD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50044">
        <property type="entry name" value="SH3-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51741">
        <property type="entry name" value="F_BAR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0206">Cytoskeleton</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-BAR" evidence="8">
        <location>
            <begin position="60"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="386"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="461"/>
            <end position="806"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="226"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="33"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="44"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="465"/>
            <end position="506"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="557"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="613"/>
            <end position="622"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="624"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="636"/>
            <end position="646"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="665"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="706"/>
            <end position="738"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="768"/>
            <end position="790"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="791"/>
            <end position="804"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS63250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1043" mass="113386" checksum="3E1967BDA9C35F00" modified="2012-04-18" version="1">MSSRPSSRNHHHQQQQQQQHPHYEKQSTATTQSHSRSNSRRSSVTNGLNNQPSSLPPDPYSFCNSFWGEHGYDALGNKNRHSSKLLDDLRLWYKERASIEEEYAKRLTKLTKNPLFEIHHHSNNNELEVGGIKRALETVRDTTRQSAHSHLELAGTIRTALEKKVAEFVTRREGLKKNPQAAIERLHKKKTELVELTEKSRKKYEANAIAVNGLSAQTHLVQGRDYDKVTAKLEKAQQNVQMDEREYRSHVKNLKETTAEWNMQWKGYCDLVQDLEEERIDFIRTSFWDYANGVSTICVVDDEQCEIIRKALERCDTAQDVAEFVRQARTGSDIYVAPEYVNYARGEQTPYNRAPAVHANFIRSSIRNPRAVPSTTNIQDLAHAIRSGPASPTDPVLPPAASSRSTSKAAVKRGGAIADAVAANSFTSMEPNPGLVPRASISRSSMGASISRAASPAEAMAQVVGSGTSDSLNSRQSVQSTPTASSVAPPQHFQNNHSNSQLTNPPRQHYEPNASKQEPTGPPPPPASAKPANRYTSGYGNKEPAHTASGLPAPSNRPVSRASTNGGVQPPSVPAENGQVDPLVAALEKLRSTPSISSFRRSPAPSATPARGRQHDEQDGRRSTLAQPFLNGQHNRASSPAPSARRSTLEQPSSAHDPYKRSHSPAVSTRRSTLEQPSSAHDPYKRSHSPAVSTRRSTLAEPQAGNMYQRSSSPGGQRTTLEQPSSYDPYKRSSSPAVSASMMKPPSDMRSNGGRSPSPQPHGGYNRGGTPSPAPSSSGQAPMSYQQQQQARHQSYGHPTSQSMPLGVALDARGGVMNQAGTGEPSNSHATPKAYSSSSWTQLTQLIQLLELSSQTQLIQLTQLSNAAHPALGPSSLRSLSSSSSQTQLLRLSQLSQLIQLLEPDHTAHPALGPSSVGPSSAGLRPQLIQLIQLSDPADPALIISSSSSQIQLIQLSDPAHPALIFSSSNLTQLIPSSFSSGNQPATMPEEFSFQANDIIAITQTDPDGWWQGELLDDFRRKQNSANGNGGNVLPSNFVDLLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>H6QSN8</accession>
    <name>H6QSN8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21740</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EHS63774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889477.1">
        <property type="nucleotide sequence ID" value="XM_003889428.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QSN8"/>
    <dbReference type="GeneID" id="13541910"/>
    <dbReference type="KEGG" id="pgr:PGTG_21740"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21740"/>
    <dbReference type="HOGENOM" id="CLU_130551_0_0_1"/>
    <dbReference type="InParanoid" id="H6QSN8"/>
    <dbReference type="OrthoDB" id="2496062at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS63774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="127" mass="14367" checksum="21D2C481F34C13F0" modified="2012-04-18" version="1">MALTAGGSQAAAPSPNQVWSCMVAKSPPPPWKQNLKALTWSQFQSGVIERLSPVEDYFDRFLKRQNLAGLIHWIAMISGNSTYSIKNAVEITSPQDFKLFVREALLRFPAKVIFRLEMNDPRETKRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTF3</accession>
    <name>H6QTF3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22078</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178312">
        <property type="protein sequence ID" value="EHS64168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889213.1">
        <property type="nucleotide sequence ID" value="XM_003889164.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTF3"/>
    <dbReference type="GeneID" id="13541724"/>
    <dbReference type="KEGG" id="pgr:PGTG_22078"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22078"/>
    <dbReference type="HOGENOM" id="CLU_1619874_0_0_1"/>
    <dbReference type="InParanoid" id="H6QTF3"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="52"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="89"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="164" mass="17792" checksum="C864DA1EFC120050" modified="2012-04-18" version="1">MVNTWSENPELITPNFTARVTPPRTTNPSVVDRSFDASIFQSAENSFIGMADNSTNSPSVPSGQNQIPFPSTSTPQHTPQDPPMTHPSTPEEHANSREPGKLTEETKDGQSIPSRSLEQIIAGELEKSGLSGIDDNLLKVLLIRNLTRSHIDSEQATVSSKSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUF2</accession>
    <name>H6QUF2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_22428</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178338">
        <property type="protein sequence ID" value="EHS64621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888863.1">
        <property type="nucleotide sequence ID" value="XM_003888814.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUF2"/>
    <dbReference type="GeneID" id="13542432"/>
    <dbReference type="KEGG" id="pgr:PGTG_22428"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22428"/>
    <dbReference type="HOGENOM" id="CLU_1826266_0_0_1"/>
    <dbReference type="InParanoid" id="H6QUF2"/>
    <dbReference type="OrthoDB" id="10269079at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="141" mass="16738" checksum="595B2540C1209C4F" modified="2012-04-18" version="1">MIGYPEGPRRILAFRRLLHRHTIASNWRELRLQLPTDPDFIETLWRRIQIDDLKKRFGISEDPLLVQDTPRLSILGWNPGKRFLSERHDIRSTLEAVFGEHEGKKRRILNSYLLQQSYPVEWWTTALTKESGLFGLDILQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>H6QUG1</accession>
    <name>H6QUG1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_22440</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178339">
        <property type="protein sequence ID" value="EHS64624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888848.1">
        <property type="nucleotide sequence ID" value="XM_003888799.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QUG1"/>
    <dbReference type="STRING" id="418459.H6QUG1"/>
    <dbReference type="GeneID" id="13542436"/>
    <dbReference type="KEGG" id="pgr:PGTG_22440"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22440"/>
    <dbReference type="HOGENOM" id="CLU_016625_0_0_1"/>
    <dbReference type="InParanoid" id="H6QUG1"/>
    <dbReference type="OrthoDB" id="2504200at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="17"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="721"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="861"/>
            <end position="883"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="861"/>
            <end position="874"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS64624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="905" mass="103054" checksum="197816100CCB5348" modified="2012-04-18" version="1">MICIDLQFDKKCFQFSVVWPLVVVVSFSWLLYVCMLLFCFFGLEVRVEAKTKSNKNHPIHSFGGLMNNSSWSRWTHLIINSNTSRTTTSRLLSTNNNNNNKPLQLLIQHALLHRHLDPIQFQRSSSGQAQLDNLLLGSLILNDHRRANYYLNRIEASHSTHQNDQFYPNLFKVCGLIGRGMGMMRRVVKAQLSASHSNQRPLRPAQIKIIEAYIENLIRWTQIERAWSLLASFDFGPSIDLSLQAELIGGRFSKRVKRIKKPPDSNKPLWKPNSSAYSSLLSIYLILDRPDLALKLNSNIDLSKIDPCMALLVCARASTSSHHQEEHPLHIDSITPPGPITTTVLAKYIYRKRGPIGFIRWLEQQTKIDSSNRILLSAWLDILLLPSEPALEPVELAEWIERIDQHDLLGERELERYLRYTREWLESQANSKIRLNLSQRRWSLDNPNRHPSIDRPISIDPDDPRLGYPQLINLFKKVYHQKLPSSSSSSRTTTSENNEFIKPSTVVVSEMVRILSLIGTPPGKLTELLDREVQENQSQIRSTHMIGLIWSKIVNHDLNGLNTLIDKEMKLKYKLVLTGLLTTIILAGLIATRAPKPMIIRISQKLIEQCPTTSPSNQQNPQNYYKLNQHTDLESVESYLNLIKAAEAVGDLILIKELHESLVAKFGREKFFGTDRPVSIDYLHLIFQAYMKLNEPLLAQTEVSLWFESLQEHYLASIPLADPPPSLPSSTTTTTTTDREESKRFLSSGVLQDFLDSQPGSKLIESDDLRGDGADERQKYGKFLESLTRSSMLIAKKLDSLGRLRRLFARTDALGLRKGEEEWEAARLAEIQARLLKFRRAQELNLELIIRFQSFLPCALPSPSSSAPTTPESPIQSCNVDGLDDQLEPLEEKKIIPRPDPSSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>H6QV63</accession>
    <name>H6QV63_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22652</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178377">
        <property type="protein sequence ID" value="EHS62724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888593.1">
        <property type="nucleotide sequence ID" value="XM_003888544.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QV63"/>
    <dbReference type="GeneID" id="13542341"/>
    <dbReference type="KEGG" id="pgr:PGTG_22652"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22652"/>
    <dbReference type="HOGENOM" id="CLU_1897232_0_0_1"/>
    <dbReference type="InParanoid" id="H6QV63"/>
    <dbReference type="OrthoDB" id="2511763at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="83"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="87"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="134" mass="14281" checksum="2E590646885611EA" modified="2012-04-18" version="1">MLNLLMHSGFPKTGGPTSCVRRCMDMSNLTQVLPCSTWGADDQLVLRALKAELSAPIGSVCFRGASSARGCVVHPSALAVFAGSVPPDSPSATSPPPRLKTRHQPQKLETSGVHQLHHILIKHQTNPVKISPTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>H6QV77</accession>
    <name>H6QV77_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22693</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178381">
        <property type="protein sequence ID" value="EHS62778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888587.1">
        <property type="nucleotide sequence ID" value="XM_003888538.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QV77"/>
    <dbReference type="GeneID" id="13542354"/>
    <dbReference type="KEGG" id="pgr:PGTG_22693"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22693"/>
    <dbReference type="HOGENOM" id="CLU_2334659_0_0_1"/>
    <dbReference type="InParanoid" id="H6QV77"/>
    <dbReference type="OrthoDB" id="10299080at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="8"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="98" mass="11071" checksum="BD1ABC1A718D47A4" modified="2012-04-18" version="1">MKKNVIVDSDEDDDFSSDNEDSNPKSEHQDQPIGPPGSAPPDQMFEDPKEMVQSVKKFACDHGYMISLKTTKQVMRLEKLLNAPEEVFQLKPINPLPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>H6QVM7</accession>
    <name>H6QVM7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_22798</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS989973">
        <property type="protein sequence ID" value="EHS63383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890874.1">
        <property type="nucleotide sequence ID" value="XM_003890825.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QVM7"/>
    <dbReference type="STRING" id="418459.H6QVM7"/>
    <dbReference type="GeneID" id="13541072"/>
    <dbReference type="KEGG" id="pgr:PGTG_22798"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22798"/>
    <dbReference type="HOGENOM" id="CLU_1603552_0_0_1"/>
    <dbReference type="InParanoid" id="H6QVM7"/>
    <dbReference type="OrthoDB" id="2506822at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125">
        <property type="entry name" value="F21J9.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125:SF3">
        <property type="entry name" value="NO-APICAL-MERISTEM-ASSOCIATED CARBOXY-TERMINAL DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="43"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="15"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="53"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="166" mass="18612" checksum="EBA60C08B5EDEA89" modified="2012-04-18" version="1">MDSNMPLQHQSTKHQTIPGPKVKASLMEPLLRDLGPTLASQKAFLASSGPAERAAKKQTDKDNTPKPDGTKPKNPLWTIEEDKLLCVAWLNVTRDPIVGTGQKAATFWERVAAYQKKLVEDYNTEKKRTKGFKELPFRLTNATKCRQGHIMKVCSRFGGCYGKVKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3JPZ8</accession>
    <name>E3JPZ8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">ATP synthase F(0) complex subunit e, mitochondrial</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_00037</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Subunit e, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro. Part of the complex F(0) domain.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) and CF(0) have multiple subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Mitochondrion inner membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the ATPase e subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74081.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307087.2">
        <property type="nucleotide sequence ID" value="XM_003307039.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JPZ8"/>
    <dbReference type="STRING" id="418459.E3JPZ8"/>
    <dbReference type="GeneID" id="10527506"/>
    <dbReference type="KEGG" id="pgr:PGTG_00037"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00037"/>
    <dbReference type="HOGENOM" id="CLU_159435_2_0_1"/>
    <dbReference type="InParanoid" id="E3JPZ8"/>
    <dbReference type="OrthoDB" id="2125027at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005743">
        <property type="term" value="C:mitochondrial inner membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045259">
        <property type="term" value="C:proton-transporting ATP synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015078">
        <property type="term" value="F:proton transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015986">
        <property type="term" value="P:proton motive force-driven ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008386">
        <property type="entry name" value="ATP_synth_F0_esu_mt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05680">
        <property type="entry name" value="ATP-synt_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4 11" id="KW-0138">CF(0)</keyword>
    <keyword evidence="5 11" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7 11" id="KW-0406">Ion transport</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="6 11" id="KW-0999">Mitochondrion inner membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 11" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004273"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007333"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU367005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP74081.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="90" mass="10284" checksum="D61C048DDA9331BC" modified="2012-04-18" version="2">MSPSATVNVVRYSAITFGILYGIVHKQTLKSKYEDLHYQEEVHKRENWVKEAKRAWQSRQLSNSAGVNNDPDSPSFDLEAFLNQLSTANQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQ57</accession>
    <name>E3JQ57_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_00096</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307146.1">
        <property type="nucleotide sequence ID" value="XM_003307098.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQ57"/>
    <dbReference type="STRING" id="418459.E3JQ57"/>
    <dbReference type="GeneID" id="10546761"/>
    <dbReference type="KEGG" id="pgr:PGTG_00096"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00096"/>
    <dbReference type="eggNOG" id="KOG1721">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_075838_1_0_1"/>
    <dbReference type="InParanoid" id="E3JQ57"/>
    <dbReference type="OMA" id="CENKHAL"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000977">
        <property type="term" value="F:RNA polymerase II transcription regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.60">
        <property type="entry name" value="Classic Zinc Finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003604">
        <property type="entry name" value="Matrin/U1-like-C_Znf_C2H2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022755">
        <property type="entry name" value="Znf_C2H2_jaz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24408:SF58">
        <property type="entry name" value="TRANSCRIPTION FACTOR (TFIIIA), PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05150)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24408">
        <property type="entry name" value="ZINC FINGER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12171">
        <property type="entry name" value="zf-C2H2_jaz"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12874">
        <property type="entry name" value="zf-met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SMART" id="SM00451">
        <property type="entry name" value="ZnF_U1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="103"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="domain" description="C2H2-type" evidence="6">
        <location>
            <begin position="203"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP74140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="284" mass="33542" checksum="F3E84D4A14440CBB" modified="2011-01-11" version="1">MSGVECEPCGRHFRLYQHYEDHLIHSPRHHYCEPCQRDFTSQNAKENHLRHSEQHMICKWCQTTVGKLRIHNQRYHEQCSECSQWCEDADEVHQHCRLAHSDVYCPPCRRIFSNPNNLDMHERSSIHQAKNVKCPHPTCERGFISKAALVQHFEAGTCQSDLDLEDVDEIFSSRCDRDQQFVDRDRIFPAARAELSCDYDGRYQCSLCPKSFGNQGQLIAHFKSPKHKNRGWKAYECPSERCGRSEYYSLGNLMFHLETGDCEEHYRRDLSKRVDKLFNIFKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>E3JR36</accession>
    <name>E3JR36_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00390</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74434.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307440.2">
        <property type="nucleotide sequence ID" value="XM_003307392.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JR36"/>
    <dbReference type="GeneID" id="10546462"/>
    <dbReference type="KEGG" id="pgr:PGTG_00390"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00390"/>
    <dbReference type="HOGENOM" id="CLU_447680_0_0_1"/>
    <dbReference type="InParanoid" id="E3JR36"/>
    <dbReference type="OrthoDB" id="10322403at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005643">
        <property type="term" value="C:nuclear pore"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016024">
        <property type="entry name" value="ARM-type_fold"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48371">
        <property type="entry name" value="ARM repeat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="39"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="381"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="541"/>
            <end position="610"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="48"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="395"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="569"/>
            <end position="610"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP74434.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="610" mass="68034" checksum="9ABC663D666D3F13" modified="2012-04-18" version="2">MDNMFGHPSECSLLGLNNDFTPLENSYLQYAGDGQLNGQFYQPIDHSPNPSLQPVPLSQVNNALPAPTQQASPNPTESISGPNPTESISGPRSHIDVDPALSGLTEPSDSSPAIPSHPADKPLPTDDSHQAPHESPTKPKPPPRKTMAPNQVQRAQNMDLDELIKLVASTAQYTRLTAENEVELNDAYMEYQRQLYLIAYKNKLAIAPCLQYVGEGINPRGASNYNNFCRYDLVASKVAQDKTMHVEDRMRELSRLWHLNDDTAKEQWKDPEFLKSLPNFTEVNKDASTSGTQKHKPPTFDADRWSNKIVADLRNLSRSSGIEGFLVVGSRSKDSSLNFQGGSHLGKRFLDMYATKDDPVSNFMDFIKGFKVIQKITGSEPPPVVTKKKPRKPKPREIITPHDKGSRKKNIKFIRDKLNAAIKLATHGTWTKGWPGTRTQWQLARLKVTLQVKQDNDLNVSPIHFCRRPGDMLEIPAQMVVAAIEEDWVQLIGPPAPSNDSVGCDIDSPANAATTTNTATTTNTATTTVSVAQCAIDNLPIKQKKRNTGPATLKANPPKKKQKRASNQEGEEEEEEEEEEEEEEEEEEEEEEDEEEEEEEEEEVVDEQDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>E3JR37</accession>
    <name>E3JR37_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GTP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_00391</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">GTPase involved in activation of the TORC1 signaling pathway, which promotes growth and represses autophagy in nutrient-rich conditions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Component of the GSE complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the GTR/RAG GTP-binding protein family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74435.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307441.2">
        <property type="nucleotide sequence ID" value="XM_003307393.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JR37"/>
    <dbReference type="FunCoup" id="E3JR37">
        <property type="interactions" value="387"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JR37"/>
    <dbReference type="GeneID" id="10528035"/>
    <dbReference type="KEGG" id="pgr:PGTG_00391"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00391"/>
    <dbReference type="eggNOG" id="KOG3887">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_047421_0_0_1"/>
    <dbReference type="InParanoid" id="E3JR37"/>
    <dbReference type="OrthoDB" id="26136at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000329">
        <property type="term" value="C:fungal-type vacuole membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990131">
        <property type="term" value="C:Gtr1-Gtr2 GTPase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009267">
        <property type="term" value="P:cellular response to starvation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010507">
        <property type="term" value="P:negative regulation of autophagy"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002877">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006762">
        <property type="entry name" value="Gtr1_RagA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259:SF2">
        <property type="entry name" value="GH16429P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11259">
        <property type="entry name" value="RAS-RELATED GTP BINDING RAG/GTR YEAST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04670">
        <property type="entry name" value="Gtr1_RagA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="282"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="5">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="282"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="322"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU367014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP74435.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="432" mass="48186" checksum="A973C9E414826FCC" modified="2012-04-18" version="2">MDGSISSLPTQTTPTPLGLPPSESSRPDRPKLLFMGPRSGGKSSIREVVFHKLQPDQTLHLETTRRITQDDIKSFIDFEIWDLPGPLDSDVLTLPFAHVGAVIFVIDAQSSYLSTIPQLASTVIQAYQTNPALHFEIFMHKIDAVTPDYRSEIIEQVKDRLTDELSDRELSIYGVPTIAIELSHRITYKLTTVFDYSIYDALSRVIMKIVPQQAALEHLMNLLAQQSGVENCILFDTWTKLYIATDSSPTEKPLLEMCTEFMDIFTDLSELYGVPQQISPVTRPTSMTNGLGKNAPMVSTRQVPDSSHTKRSLSRGRASVLSPATRSSSVAVDQNKKPASLAGKRMITSQVKVDTSFGGPRGIGMPGIEQPGRVISYYEINQFLTLVCITHEEIMRTQGTLIEYNVSRVRESIEKIFQITNGTPSKHRRRVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3JSH4</accession>
    <name>E3JSH4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CCHC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_01540</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP74947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319366.1">
        <property type="nucleotide sequence ID" value="XM_003319318.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JSH4"/>
    <dbReference type="GeneID" id="10547229"/>
    <dbReference type="KEGG" id="pgr:PGTG_01540"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01540"/>
    <dbReference type="HOGENOM" id="CLU_051119_2_0_1"/>
    <dbReference type="InParanoid" id="E3JSH4"/>
    <dbReference type="OrthoDB" id="2498930at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032865">
        <property type="term" value="C:ERMES complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007005">
        <property type="term" value="P:mitochondrion organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027532">
        <property type="entry name" value="Mdm12"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28204:SF1">
        <property type="entry name" value="MITOCHONDRIAL DISTRIBUTION AND MORPHOLOGY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="191"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="196"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP74947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="291" mass="32956" checksum="B32DA37779814689" modified="2011-01-11" version="1">MATEVIRETKVLLTPNNYALWLLPMEARLFRLKLLDILEGQVQPDEKEQEKWTKLNNDAYAEIIAYIGQEVLGFVSSALPATPRFNGRGLWKLLKSQYAGDDLTSQTTALDQFLHLGFSSIATFIPAVRSGNQKISMSGLVLDDRVRTILMLKKLPSDYRSFRDIVSMNYGTESFESVIKKLESYVAQNDLDKKPSSTSLTPPQSLLTQSNDQAASSSTLCDHCKKPGHRINNCWAKYPEKAPKSHSAHMTVQDNFNQLNDPTDFSRFRTADGKLHHVDEMRFEGVQYFNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3JSP1</accession>
    <name>E3JSP1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_01659</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75066.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319485.2">
        <property type="nucleotide sequence ID" value="XM_003319437.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JSP1"/>
    <dbReference type="GeneID" id="10547428"/>
    <dbReference type="KEGG" id="pgr:PGTG_01659"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01659"/>
    <dbReference type="HOGENOM" id="CLU_1918102_0_0_1"/>
    <dbReference type="InParanoid" id="E3JSP1"/>
    <dbReference type="OrthoDB" id="10273393at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP75066.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="132" mass="14088" checksum="8FB0DEC3CE81619C" modified="2012-04-18" version="2">MPWIKADGVAQSLAASPRELSGACWQASPALPRMRISGPADQYWSGPAGPKFALELGRLSPAAAVPTMKPTAHSASSITWLKGRTRSICDMAQEKKKLLRRTKDKARAASQKLGTADRLALRESGIGIDGSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUE6</accession>
    <name>E3JUE6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Zinc-finger domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_01002</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75671.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320090.2">
        <property type="nucleotide sequence ID" value="XM_003320042.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JUE6"/>
    <dbReference type="GeneID" id="10543551"/>
    <dbReference type="KEGG" id="pgr:PGTG_01002"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01002"/>
    <dbReference type="HOGENOM" id="CLU_609934_0_0_1"/>
    <dbReference type="InParanoid" id="E3JUE6"/>
    <dbReference type="OrthoDB" id="2497737at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018866">
        <property type="entry name" value="Znf-4CXXC_R1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10497">
        <property type="entry name" value="zf-4CXXC_R1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Zinc-finger" evidence="7">
        <location>
            <begin position="136"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="30"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="233"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="293"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="355"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="383"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="72"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="233"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="293"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="362"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF10497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP75671.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="449" mass="49851" checksum="FD3995B4B764BBA3" modified="2012-04-18" version="2">MTVFGDDDIIEMRVGPRHFTNGNRRAVAVKREALDKNRLNGSPRSVGERSSERGPTPANASFTFGGDHGRTGVQTTESLRTTSGVKSSLHTHQRATFTAIPSTTAPPTHGSCKSKSQAKRPVPSPATAPDATGQYESSTCHQCRTKTTRPKMICDQSLNPNCLVRICRPCLMDRKAYDEMPKLRGPLFKFVPGGKILCMKCRNICPCASCRRARGERGVMGNGLNGFYDLMRDEPKTEAPLRKTKRKEREQAKPKQSPGHPMVDKCKNEQKGAEVVCLEESWDELVGKSNRTIISGKNGKTTGQSHARSNHIGSRASCGVRKSPDPVWIAGCSRKRKFCAKQFTDEESDCCIPVDGPTIPAPKKEKPGFRSKDPEDVRQDRLERQLDSQIRKLELRRAREELERNNATADAFTKAKMIQQFLRCGLNLEDALRATNECLCLPTQSPALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3JUP5</accession>
    <name>E3JUP5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribosomal RNA-processing protein 8</fullName>
            <ecNumber evidence="8">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_01101</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <text evidence="8">S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N(1) position of adenine in helix 25.1 in 25S rRNA. Required both for ribosomal 40S and 60S subunits biogenesis. Required for efficient pre-rRNA cleavage at site A2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Nucleus</location>
            <location evidence="1 8">Nucleolus</location>
        </subcellularLocation>
    </comment>
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    <dbReference type="SMR" id="E3JUP5"/>
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    <dbReference type="STRING" id="418459.E3JUP5"/>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01101"/>
    <dbReference type="eggNOG" id="KOG3045">
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    </dbReference>
    <dbReference type="GO" id="GO:0005730">
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        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="term" value="F:rRNA (adenine) methyltransferase activity"/>
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        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
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    </dbReference>
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        <property type="entry name" value="RRP8_N"/>
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    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12787">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12787:SF0">
        <property type="entry name" value="RIBOSOMAL RNA-PROCESSING PROTEIN 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05148">
        <property type="entry name" value="Methyltransf_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7 8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6 8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 8" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="56"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="66"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365074"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP75770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="386" mass="43557" checksum="AB5140CE626D02CD" modified="2011-01-11" version="1">MKKPTNKLLFDTPGWTIPKENINSEKKAKRKKNRNKPTTERPSSSEQQQEDSPSPKRLKNSHPHNNKQQQQQQPTKNQPNETLHVGTHQLAKGLLNSNLNGSRFRILNETLYTSTGPEALKLFQSNPIEAAADDEEEEGGGEHRIRREENPNFEIYHLGFRSQTKHWPQNPVDLIAHQLQQDPHLQKIPGPVLVADLGCGEAPLAKLLCSSPSTSSSNQKNSKPIAHHNQFKVFSYDLVADREGWITVAECSSLVPLPGSLDDRVGNGMMDIVVCCLSLMSTNWVGMILEARRILKHDGELRIAEVTSRFVDVDLFVTFIKSIGFSNPTKDQSNTHFILFKFKKLPLTLAKNQRDSSSVDLLNPQSKLELIQTGQKLLKPCIYKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3JV25</accession>
    <name>E3JV25_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_01231</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75900.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320319.2">
        <property type="nucleotide sequence ID" value="XM_003320271.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JV25"/>
    <dbReference type="GeneID" id="10532815"/>
    <dbReference type="KEGG" id="pgr:PGTG_01231"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01231"/>
    <dbReference type="HOGENOM" id="CLU_1200331_0_0_1"/>
    <dbReference type="InParanoid" id="E3JV25"/>
    <dbReference type="OrthoDB" id="2507016at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="110"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="113"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="130"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP75900.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="231" mass="25074" checksum="299AA54663B68ABD" modified="2012-04-18" version="2">MATQSVTNSTLPATGLPRSTSAGTISARQTSSSTASIPPQTQTSSSPAASASALSSIFTKQNAPSAPVTQHTDPFTRLSPRAIAAIVFVGAFVTVLCLAISFVLWRRKDRNPRSNTNDPETPSTVQCQKTPRHSKRKKSQRRPVGLVDETTTTFVNSIRPPSPVRARMTYIDQTSTSPPWSVFLGRGNSSILNWKPHDGMRPPLRLSSDTLMVLDPWLMRRSQSPPPPLPS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="72" xmlns="https://uniprot.org/uniprot">
    <accession>E3JVX0</accession>
    <name>E3JVX0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">threonine synthase</fullName>
            <ecNumber evidence="4">4.2.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_02636</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the threonine synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.1" evidence="4"/>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320614.1">
        <property type="nucleotide sequence ID" value="XM_003320566.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JVX0"/>
    <dbReference type="FunCoup" id="E3JVX0">
        <property type="interactions" value="117"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JVX0"/>
    <dbReference type="GeneID" id="10533763"/>
    <dbReference type="KEGG" id="pgr:PGTG_02636"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02636"/>
    <dbReference type="eggNOG" id="KOG2616">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015170_1_0_1"/>
    <dbReference type="InParanoid" id="E3JVX0"/>
    <dbReference type="OMA" id="NFERYLY"/>
    <dbReference type="OrthoDB" id="5203861at2759"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00065"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004795">
        <property type="term" value="F:threonine synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd01560">
        <property type="entry name" value="Thr-synth_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000024">
        <property type="entry name" value="Probable threonine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1380.10:FF:000003">
        <property type="entry name" value="THR4p Threonine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1380.10">
        <property type="entry name" value="Threonine synthase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029144">
        <property type="entry name" value="Thr_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037158">
        <property type="entry name" value="Thr_synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004450">
        <property type="entry name" value="Thr_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051166">
        <property type="entry name" value="Threonine_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00260">
        <property type="entry name" value="thrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42690">
        <property type="entry name" value="THREONINE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42690:SF1">
        <property type="entry name" value="THREONINE SYNTHASE-LIKE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24857">
        <property type="entry name" value="THR4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14821">
        <property type="entry name" value="Thr_synth_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 9" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Threonine synthase N-terminal" evidence="12">
        <location>
            <begin position="2"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="11">
        <location>
            <begin position="103"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="316"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="10">
        <location>
            <begin position="325"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9">
        <location>
            <position position="113"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR604450-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF14821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP76195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="573" mass="63749" checksum="E264D4FAA0E89661" modified="2011-01-11" version="1">MEYISTRGGGESLTFEQTVLSGLAPDGGLYIPKRIPELPKDWESRWSQLSFTELALEIYSLFIPSEEITRAELKELIERSYSTFRHPAIAPLKKLTPSRYVVELWHGPTFAFKDVALQFLGNLFEFFLNRKNLNRPKDSPAERITVVGATSGDTGSAAIYGLRGKNDISIFILHPKGRVSPIQEAQMTTVLDDNVFNLAVNGTFDDCQDIVKTLFNDTEFNSTYRLGAINSINWARILAQIVYYFSSYYALRHTLKPKQALQFVVPTGNFGDILAGFYAHKLGLPIGINGTTPGLVIATNSNDILTRFWETGKYEKQASTDDEQSSPNHTTVQTAPVVGSSDGKQASDPKHGFVKETLSPAMDILVSSNFERLLFYLAFDTLTESKDPEEERIKSSQAKVKEWMSQLKSTGRFRVPESTLQAARALFSAGRVDDEQTKQTIRRYFNADKDVLEDGQRYVVDPHTAVGLFVAENLKPGGQEEVLQAILSTAHPAKFAEAVTQSILTQQASSDHPPSSFDFERDVLPVQFIGLLDKPRRVIDVHGIEIQLTKDVIISHSNALISPSSNLDPTTSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3JVX3</accession>
    <name>E3JVX3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02639</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76198.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320617.2">
        <property type="nucleotide sequence ID" value="XM_003320569.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JVX3"/>
    <dbReference type="GeneID" id="10533766"/>
    <dbReference type="KEGG" id="pgr:PGTG_02639"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02639"/>
    <dbReference type="HOGENOM" id="CLU_2688993_0_0_1"/>
    <dbReference type="InParanoid" id="E3JVX3"/>
    <dbReference type="OrthoDB" id="10342393at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="29"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="65"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76198.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="74" mass="8278" checksum="005F56CE0FE2448A" modified="2012-04-18" version="2">MCVSTRLDRAGSSGECVWMVTSTVVRRVKTKKKAEKKQSQDQEASKQPTNRADFFWGVDTGNGWDVSSNQDGYC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3JWI8</accession>
    <name>E3JWI8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">NodB homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_02854</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Lipid-anchor</topology>
            <topology evidence="2">GPI-anchor</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178265">
        <property type="protein sequence ID" value="EFP76413.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003320832.2">
        <property type="nucleotide sequence ID" value="XM_003320784.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JWI8"/>
    <dbReference type="GeneID" id="10534468"/>
    <dbReference type="KEGG" id="pgr:PGTG_02854"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02854"/>
    <dbReference type="HOGENOM" id="CLU_021264_11_2_1"/>
    <dbReference type="InParanoid" id="E3JWI8"/>
    <dbReference type="OrthoDB" id="2125469at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10951">
        <property type="entry name" value="CE4_ClCDA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002509">
        <property type="entry name" value="NODB_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46471">
        <property type="entry name" value="CHITIN DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46471:SF2">
        <property type="entry name" value="CHITIN DEACETYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01522">
        <property type="entry name" value="Polysacc_deac_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51677">
        <property type="entry name" value="NODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="3" id="KW-0336">GPI-anchor</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0732">Signal</keyword>
    <feature type="domain" description="NodB homology" evidence="10">
        <location>
            <begin position="98"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="301"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP76413.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="321" mass="35744" checksum="A5074A2F2C6D8CCC" modified="2012-04-18" version="2">MMMMMMAQSAGIKKSKQWFTSMIRPVMILVIHVLALTVVQPRQHTPAFVLNNIDADSQDARQVMGPDANHNQLPFISALDTARADFAVPVYTTCSAPGSFALTFDDGPSEFSSNLDGTLDGSNSKASFFINGNNFGCIYDYAERLVDRFNKGHLIASHTWSHVHSNEGTYDQIAFQLELIENAMIKILGVKPLYFRPPYGEYNEVVLQVLQDRGYRGLILWSEDSGDSFGTPPSSSEIIESYRSYPEQTNVLNHETKSFTVNEVIPNVIPILKDKGFDLQTVPHCLNLGSNPSDWYVSVQPPGSRDETWTCDGTPMPGSFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXT4</accession>
    <name>E3JXT4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DUF1899 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_02320</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the WD repeat coronin family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321278.1">
        <property type="nucleotide sequence ID" value="XM_003321230.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXT4"/>
    <dbReference type="STRING" id="418459.E3JXT4"/>
    <dbReference type="GeneID" id="10529044"/>
    <dbReference type="KEGG" id="pgr:PGTG_02320"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02320"/>
    <dbReference type="eggNOG" id="KOG1445">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_006604_0_0_1"/>
    <dbReference type="InParanoid" id="E3JXT4"/>
    <dbReference type="OMA" id="TIMYMEV"/>
    <dbReference type="OrthoDB" id="347435at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003779">
        <property type="term" value="F:actin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:001012">
        <property type="entry name" value="Actin cross-linking, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.130.10.10:FF:000774">
        <property type="entry name" value="Coronin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015505">
        <property type="entry name" value="Coronin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015048">
        <property type="entry name" value="DUF1899"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856">
        <property type="entry name" value="CORONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10856:SF20">
        <property type="entry name" value="CORONIN-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16300">
        <property type="entry name" value="WD40_4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01166">
        <property type="entry name" value="DUF1899"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01167">
        <property type="entry name" value="DUF1900"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82171">
        <property type="entry name" value="DPP6 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0009">Actin-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="DUF1899" evidence="8">
        <location>
            <begin position="503"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="402"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="970"/>
            <end position="1020"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="410"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="423"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="445"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="7">
        <location>
            <begin position="980"/>
            <end position="989"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="7">
        <location>
            <begin position="996"/>
            <end position="1005"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP76859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1020" mass="111811" checksum="85942D31F6B44A49" modified="2011-01-11" version="1">MPPSRFAQKEHLSYRNLLAIPAKKEVQFSEIPVLTNGTDSEDLIAATSQYWITKHTNQGHLTFAPYHKPGKLANKTASLSACSGSISDFTTCPFDQLLAVANEAGETSIFSLPETLNPPGDTQAFNCIRKLHGESKNSPVDKLAFHSTTRGILAGSIGTQLAIWDAEHLSEGSPSINLASGAKVWDIKWGWDGRLLGATTRTGILQLWDPRASTMVSSCDNQDGSGGRKCSRLAWVGDHILTTSVNKLRDRQYSIFDPRSLNSPVKTERIDNSNGTLIPIVDPNRSILYLATRGESTFKWYKLNLSSTLTVEAFNSMLPMSPIAGIAMAPINHQTVDVMRTELCKFMILTKSAEVIPLTIQAPKRQYLDFHADVMPSVRSMEPAQAGQDWLKGNDQTVALVSQDPSAPGSIKSSIKSTPISTALQPTPASGIVQSSIKSAPATATMHQESPQAPGSPKNQLPATGPASYGVKKSFDAKPSDPAPSQSVVKPISKKGVVNKPVRWSRKYVNGQTSMQAEYEDLHNLSATFPADREMIKCTSSFFLVPIGGPGGRLGVHRLTNKGRLPTRLPCLINEANIVAFEVDQLVQGRVFVAGEDGKVRVFRVPEQGLEEDSLNAELILSASSMDRISLIKPHPTAKDILLTISDDMNKPCVRLWYVGQVGDTASGPSREIHLPSGAISSASWSLDGSLLAIANKAKTLYVLDPRRGDDTHWCQGSTHGSSRPVQVTWTDDRSHLLTTGFSSTGMREVKLYQVNQEQHAIESLAAISFDNSPAGFFIHYDPDTSIGYCWSKGERTTYLIELIETPNPNANPGQSTFKFDRLTPFVHSTIQLGYSFFIKQVLDVKAVEVGRALRLTGNQVEVVSFKIPRNRAEFFQDDIFCDTRDLLTPAFQNGQDWLDLLGQNDPQEDRQEKDLIEGFSRVSLQPVGMSRLSEAPLTKVQSNQKALIAQGPQLTDSQKADQYLDKLFQSAKRDDHHQLSGSSTSQSKLAKPDKDDDDDDEEEEVVGRSRVGAPVDDDW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3JY47</accession>
    <name>E3JY47_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">t-SNARE affecting a late Golgi compartment protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGTG_02433</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Golgi apparatus membrane</location>
            <topology evidence="1">Single-pass type IV membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the syntaxin family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76972.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321391.2">
        <property type="nucleotide sequence ID" value="XM_003321343.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JY47"/>
    <dbReference type="FunCoup" id="E3JY47">
        <property type="interactions" value="265"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JY47"/>
    <dbReference type="GeneID" id="10529239"/>
    <dbReference type="KEGG" id="pgr:PGTG_02433"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02433"/>
    <dbReference type="eggNOG" id="KOG3202">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061883_0_1_1"/>
    <dbReference type="InParanoid" id="E3JY47"/>
    <dbReference type="OrthoDB" id="2502784at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000139">
        <property type="term" value="C:Golgi membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031201">
        <property type="term" value="C:SNARE complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005484">
        <property type="term" value="F:SNAP receptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000149">
        <property type="term" value="F:SNARE binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048193">
        <property type="term" value="P:Golgi vesicle transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006886">
        <property type="term" value="P:intracellular protein transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048278">
        <property type="term" value="P:vesicle docking"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006906">
        <property type="term" value="P:vesicle fusion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd21442">
        <property type="entry name" value="SNARE_NTD_STX6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15851">
        <property type="entry name" value="SNARE_Syntaxin6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.90:FF:000012">
        <property type="entry name" value="SNARE domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.5.110:FF:000006">
        <property type="entry name" value="Syntaxin 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010989">
        <property type="entry name" value="SNARE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045242">
        <property type="entry name" value="Syntaxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015260">
        <property type="entry name" value="Syntaxin-6/10/61_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000727">
        <property type="entry name" value="T_SNARE_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957:SF224">
        <property type="entry name" value="HL02043P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19957">
        <property type="entry name" value="SYNTAXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05739">
        <property type="entry name" value="SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09177">
        <property type="entry name" value="STX6_10_61_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00397">
        <property type="entry name" value="t_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58038">
        <property type="entry name" value="SNARE fusion complex"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47661">
        <property type="entry name" value="t-snare proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50192">
        <property type="entry name" value="T_SNARE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0333">Golgi apparatus</keyword>
    <keyword evidence="9 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0653">Protein transport</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="282"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="T-SNARE coiled-coil homology" evidence="14">
        <location>
            <begin position="209"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="78"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="130"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="247"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="12">
        <location>
            <begin position="79"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="167"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00073343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EFP76972.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="301" mass="33986" checksum="506EA147B4E00524" modified="2012-04-18" version="2">MSKNDPYFEVRAEVESSLLSAAALHSSYNRILLTLPPESRPTSEELSSTRTELKATLSTLIYDISELDHVVRVLEQDLSPSSSSSPTSSKKNNHLNKSKFGVSLDEVKKRRAWLVEVQQQVQAMKETVEMPLGSNPVPSSYHPLLPSPISPTDRKSGGRTASRSQSRNHEDRQKRSRNPAESRPQFRDDLESQREAQDDTEEFYHQQESMIMKQQDQTLGTISGVVGVLREQASLMGQEMSEQNVMLDELDGQIDHTESRLSKANRKLNRFVEENKNSKSSWTILILIIILTILLIAIVLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZY1</accession>
    <name>E3JZY1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">MIOS-like alpha-solenoid domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_03562</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the WD repeat mio family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77606.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322025.2">
        <property type="nucleotide sequence ID" value="XM_003321977.2"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JZY1"/>
    <dbReference type="GeneID" id="10538814"/>
    <dbReference type="KEGG" id="pgr:PGTG_03562"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03562"/>
    <dbReference type="eggNOG" id="KOG1008">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005843_0_0_1"/>
    <dbReference type="InParanoid" id="E3JZY1"/>
    <dbReference type="OrthoDB" id="341486at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904263">
        <property type="term" value="P:positive regulation of TORC1 signaling"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16691">
        <property type="entry name" value="mRING-H2-C3H3C2_Mio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.130.10.10">
        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037593">
        <property type="entry name" value="MIOS/Sea4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049092">
        <property type="entry name" value="MIOS_a-sol"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015943">
        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031488">
        <property type="entry name" value="Zn_ribbon_mio"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16453:SF9">
        <property type="entry name" value="GATOR COMPLEX PROTEIN MIOS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16453">
        <property type="entry name" value="WD40 DOMAIN-CONTAINING PROTEIN MIO FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21719">
        <property type="entry name" value="MIOS_a-sol"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00320">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0853">WD repeat</keyword>
    <feature type="domain" description="MIOS-like alpha-solenoid" evidence="5">
        <location>
            <begin position="663"/>
            <end position="729"/>
        </location>
    </feature>
    <feature type="domain" description="MIOS-like alpha-solenoid" evidence="5">
        <location>
            <begin position="814"/>
            <end position="950"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="205"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="364"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="430"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="581"/>
            <end position="602"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="731"/>
            <end position="788"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1304"/>
            <end position="1324"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="10"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="4">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="364"/>
            <end position="376"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="379"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="450"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF21719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP77606.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1324" mass="144486" checksum="4FF47BFF85333D58" modified="2012-04-18" version="2">MPQKNHHQHNNNNNNINNNNTNQHQQTTSNNNNNNNNNTNNNNNNCPSRRFTLLQDPSLNPTGKFLVVSNSELRSYQAWKVSEQQQINYRSISFRSDLPYSYKCVDWSTTRQGFDLVAAGTSTGRAFLVTLPYAHHPTSKPELYSPHPIISFSTGRSTRPCNSIAFSPCGKLLALALDKARDYGLQIYDLDRILGLSQAIASNNHEPIPPPIKNSLPPPSSPAPPSCITNQYSPELVISPHPTSSATDPHSDQLALAQAINAETVHAIEFILITPASNQPSLLAASSSKLMALYDLRSPGTKNTDHTLNPDFATGVLNPVSQWPARSILGIKADPLVAYRFATWGDDRCVKLWDSRKTSECVLQFSQDDPNPTSARGSRKTEGSIKGEHSNQILSVTWSKARKGLLATLSSDSSRVRIWDIVDGNPPVQSARNWGDGSLLNPFGTRTNLPPGSGSSSTSPASFPNVAANFRKSAIAPRGSSPSTSPREKVQLPNYTILSSRLLKPFSRPSQAIVAAHMPTLGGTTASNSSLEYCCNLLSISREGHLEFLESKSPGEASFGARGQTAYSDGVRLRIEWHPLNHTTNRPQPETQNTTSIPMSTESSNTITASIRSPPRLDLDPQQPLPLDLKTCSFQDIDNRCSINLPTAELNSGPLKNDISMVMMKRALSGYGPNPMKNEHLVPGDPVLGAFWEWITHSDKLSQQGTGLVNGYDFSFQGVLAIMKGFLPVDQQQQQQQQQSFGTDLGTPPSQRRGAQMTTPERFGVEFLRSPKPTNNNHPPPPQNPNYSKQVSQYLKALRTFNHSHQVENFTISSEFSDQRQTALFLCGPDYNSGKIEEVLTKYEQMGMYGKACAIALFSGNTQRAITSLQRSDDLQLRTLAPPLATHLNTQRHGQARDPIFDQVCRQLSDERTSEPYLRAIFAYCATGDWREVIDETGLPLKDRLAVGLRFLSDEEIFHWLEESARDAIQSGDLEGILLTGLNHAGLLKSKKTDEMLEGKVSKSRGSSDESRDLLGPGFRLIQTYVNRTGDLQTAALASHLVVPTRLREPTAERWVDSYRRLLDRWTLFNVRVELDISRGLKARQSLTTSLNSTGTTTTSSSSLFPSQAVNPNSLVIAPPQLLLRCQHCLEVLSDGPNVRAQLHRISERAPPVLIPPIVSSSSSSAAAAASVAASAFTSASASSASVSASASPVGFSNLPGNPAAIGPASSKKNMSSNNPLFVQSKQTNRCRMCGKGLPKCSICLIPLTINDSRTPSIWAWCHRCRHVGHAHHLDLWASRGNLVCPVSGCDCLCWDASGPASFPLPASSPSPSSASSPSSPSLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZZ0</accession>
    <name>E3JZZ0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_03571</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77615.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322034.2">
        <property type="nucleotide sequence ID" value="XM_003321986.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZZ0"/>
    <dbReference type="STRING" id="418459.E3JZZ0"/>
    <dbReference type="GeneID" id="10538823"/>
    <dbReference type="KEGG" id="pgr:PGTG_03571"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03571"/>
    <dbReference type="eggNOG" id="ENOG502S1YD">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029378_3_1_1"/>
    <dbReference type="InParanoid" id="E3JZZ0"/>
    <dbReference type="OrthoDB" id="2336871at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053216">
        <property type="entry name" value="Appressorial_penetr-assoc"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34587:SF2">
        <property type="entry name" value="G-PROTEIN COUPLED RECEPTORS FAMILY 1 PROFILE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34587">
        <property type="entry name" value="VWFA DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173112" evidence="2">
        <location>
            <begin position="36"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="252"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="255"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="271"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="295"/>
            <end position="304"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP77615.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="304" mass="32870" checksum="E8806DD0F0DB0AB9" modified="2012-04-18" version="2">MYNFVISHDFRGSKICCTFLALVLGLFLIDKPAHAAENQNSQSNLCLDPSLIQEAAKSNGLKDPTNPLHNTKSLTSSNNFLDFCRGARLTNGEQVPEGSCNPTPMGSIPSKQNMPSVRIIGPSPDAIIPPNADFDVDIFAHKIELGFFTSPSNTYYLAPQQLSSKGLIRGHTHIVIQRSVGRRVFETQETVFFKGVTNRTVNGQVKTPVKGGLPAGFYRISTLTAAMNHQPVVLPVAQRGAVDDAIYIRVGSSRRASKKRGGKKKSAEASDNASNTSNGVSDGASDDSSDEKKVGKSKHKKDRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3K036</accession>
    <name>E3K036_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Alpha-1,2-Mannosidase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_03617</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the glycosyl hydrolase 47 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77661.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322080.2">
        <property type="nucleotide sequence ID" value="XM_003322032.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K036"/>
    <dbReference type="GeneID" id="10539033"/>
    <dbReference type="KEGG" id="pgr:PGTG_03617"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03617"/>
    <dbReference type="HOGENOM" id="CLU_1826254_0_0_1"/>
    <dbReference type="InParanoid" id="E3K036"/>
    <dbReference type="OrthoDB" id="8118055at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004571">
        <property type="term" value="F:mannosyl-oligosaccharide 1,2-alpha-mannosidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036503">
        <property type="term" value="P:ERAD pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="1.50.10.10:FF:000130">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001382">
        <property type="entry name" value="Glyco_hydro_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050749">
        <property type="entry name" value="Glycosyl_Hydrolase_47"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036026">
        <property type="entry name" value="Seven-hairpin_glycosidases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742:SF103">
        <property type="entry name" value="ENDOPLASMIC RETICULUM MANNOSIDASE MNL2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11742">
        <property type="entry name" value="MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01532">
        <property type="entry name" value="Glyco_hydro_47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48225">
        <property type="entry name" value="Seven-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0106">Calcium</keyword>
    <keyword evidence="5" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601382-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP77661.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="165" mass="19564" checksum="D2CB6F675E348487" modified="2012-04-18" version="2">MLARSVYYMWRITGDRQWQDRGWRMFTSWMESCVTEFGFASLKDVNRWPPVLSDHQQSFVLAETFKYYYLLFSEPDLISLDEYVLNTEAHPFRLESPGKPAKRYWSKPDESVNKAYEPPGINEGERGYGTFIQQWDRVDLDKISENDLQVYNKVKGIDTEDSYIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2E5</accession>
    <name>E3K2E5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04470</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78514.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322933.2">
        <property type="nucleotide sequence ID" value="XM_003322885.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2E5"/>
    <dbReference type="STRING" id="418459.E3K2E5"/>
    <dbReference type="GeneID" id="10536195"/>
    <dbReference type="KEGG" id="pgr:PGTG_04470"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04470"/>
    <dbReference type="HOGENOM" id="CLU_031991_2_0_1"/>
    <dbReference type="InParanoid" id="E3K2E5"/>
    <dbReference type="OrthoDB" id="2498268at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071013">
        <property type="term" value="C:catalytic step 2 spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000243">
        <property type="term" value="C:commitment complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071011">
        <property type="term" value="C:precatalytic spliceosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034719">
        <property type="term" value="C:SMN-Sm protein complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097526">
        <property type="term" value="C:spliceosomal tri-snRNP complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005685">
        <property type="term" value="C:U1 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005686">
        <property type="term" value="C:U2 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005687">
        <property type="term" value="C:U4 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005682">
        <property type="term" value="C:U5 snRNP"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000387">
        <property type="term" value="P:spliceosomal snRNP assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="31"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="323"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="341"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78514.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="488" mass="53927" checksum="ABBF303730E139C4" modified="2012-04-18" version="2">MNEDGTFGQDCPSIPGPHIPVLFDGTAHLTETASTESRPILTQSSTSDQPVEIQTPGVSVSGVGSADGNKSFPVYVEFTLYVPGEEASRSPKKRRNITVPSKTPIPGGTTDKIQSENEKIVIIWKVINKDLDAFKAEVIRGIKSEEDKFLGFFVERQENARNIFWNVVIPFGGAFAGNHKRRLDSVETFKDFLLVAEGTSETRKIVCRLVQKDPKDIAKKQSVYKQLEKNHSSSSADVNNPVLVPSPSAMNTAALSQLLQEIFLVHSPSEEHSGSAETSVYINPENSDEYFLITMQKAYSWAKAIQANPEEVTVLLPPKSPIFQFKNKNNKSEQPNPPTFPNNSQPQQFYPNPNQFTMPMAYQTPMMPYGNYSGAFGNSHQANPSAFYPQASTSAVQIPPSKSNVIDPSPPPFEDTTDLSDYLKFARVDADSEEINNGLRKLGITHWSMFEVVSADELVTSGVPLVPARCLIRSVKTYPTHLKKIKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2N0</accession>
    <name>E3K2N0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04555</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78599.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323018.2">
        <property type="nucleotide sequence ID" value="XM_003322970.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2N0"/>
    <dbReference type="GeneID" id="10536367"/>
    <dbReference type="KEGG" id="pgr:PGTG_04555"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04555"/>
    <dbReference type="HOGENOM" id="CLU_004591_4_0_1"/>
    <dbReference type="InParanoid" id="E3K2N0"/>
    <dbReference type="OrthoDB" id="10354305at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78599.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="210" mass="23972" checksum="C7164F8B6B4796AC" modified="2012-04-18" version="2">MNQRHQNLVQMNKDQVARRASQRAAVEASTNETLIMDSVSDLLEPVIGMNEGQPDQVDADESELDEESEEIPPGTFVEEESDDEEAELSWMDFVGVAIGQIDSEPEDLDLPLSSPLFRREEEKIKKIRPDNSTWYPFLNKESVFTVCDVRLPRWEALRMMRVNIRTLINRTIVEKETVFGQPVFGLNASELILDVSKTYLMLSVSENHLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2V4</accession>
    <name>E3K2V4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_04629</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323092.1">
        <property type="nucleotide sequence ID" value="XM_003323044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2V4"/>
    <dbReference type="GeneID" id="10536526"/>
    <dbReference type="KEGG" id="pgr:PGTG_04629"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04629"/>
    <dbReference type="HOGENOM" id="CLU_131095_0_0_1"/>
    <dbReference type="InParanoid" id="E3K2V4"/>
    <dbReference type="OrthoDB" id="2510615at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP78673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="175" mass="19596" checksum="39E019655F9E2366" modified="2011-01-11" version="1">MIEIESCIYVPEEPPFVNRQHYRIQDSTPWACTDTTMVPILGHLFDVYTSAPRGDSDNAASWLTFQGRCIARYSEPNIAILCNSSSYHNEIPHRHRHIPYPIVRGYLKVLDQGVNCLALDPDVSDSALFRFSSKSSAIQNSLGELNPGQIVYVSAYLTQNLTGIVDLEVSTVYIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2Y2</accession>
    <name>E3K2Y2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Mitochondrial carrier</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_04258</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the mitochondrial carrier (TC 2.A.29) family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322721.1">
        <property type="nucleotide sequence ID" value="XM_003322673.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2Y2"/>
    <dbReference type="GeneID" id="10542255"/>
    <dbReference type="KEGG" id="pgr:PGTG_04258"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04258"/>
    <dbReference type="eggNOG" id="KOG0758">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_015166_16_0_1"/>
    <dbReference type="InParanoid" id="E3K2Y2"/>
    <dbReference type="OMA" id="VEMFKIR"/>
    <dbReference type="OrthoDB" id="14252at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031966">
        <property type="term" value="C:mitochondrial membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005739">
        <property type="term" value="C:mitochondrion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015187">
        <property type="term" value="F:glycine transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990575">
        <property type="term" value="P:mitochondrial L-ornithine transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.40.10">
        <property type="entry name" value="Mitochondrial carrier domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023395">
        <property type="entry name" value="MCP_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018108">
        <property type="entry name" value="MCP_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050567">
        <property type="entry name" value="Mitochondrial_Carrier"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624">
        <property type="entry name" value="MITOCHONDRIAL BASIC AMINO ACIDS TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45624:SF45">
        <property type="entry name" value="MITOCHONDRIAL CARRIER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00153">
        <property type="entry name" value="Mito_carr"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103506">
        <property type="entry name" value="Mitochondrial carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50920">
        <property type="entry name" value="SOLCAR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 9 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="19"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="111"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="repeat" description="Solcar" evidence="9">
        <location>
            <begin position="212"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU000488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP78302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="13">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="303" mass="33167" checksum="7DD57E59E03D71E3" modified="2011-01-11" version="1">MDQIQDLPSERLTSGQHHASTAASLISGSVGGTMQVLVGQPLDTIKTRAQIASPGTITGPMDVARRTLAQEGFLGFYKGMLSPLFGVAGVNSLLFGAYAVSKRIVSPYPDLTVLQTALAGSMAGAVNSVLASPVEMFKVRMQAQYGKPNDLRLREAVRLMWEEWGFRKGIMRGFWVTVAREIPAYAGFYTGFEVSKQAFQKRYGSAQTLPVWTLLCSGAMGGIGYWTCCYPLDVIKSRIQMADRPPKGINYIAETWRKICKEEGARALFRGLAPTYLRAVPAAASTFVGYELTMEFLKNHTNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3K2Y7</accession>
    <name>E3K2Y7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04263</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178270">
        <property type="protein sequence ID" value="EFP78307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322726.1">
        <property type="nucleotide sequence ID" value="XM_003322678.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K2Y7"/>
    <dbReference type="GeneID" id="10542260"/>
    <dbReference type="KEGG" id="pgr:PGTG_04263"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04263"/>
    <dbReference type="HOGENOM" id="CLU_858260_0_0_1"/>
    <dbReference type="InParanoid" id="E3K2Y7"/>
    <dbReference type="OrthoDB" id="2509909at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003172975" evidence="1">
        <location>
            <begin position="24"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="324" mass="37028" checksum="704E95E51426082C" modified="2011-01-11" version="1">MSFFHLLFLLLGVQKLVAPPSLGSDWYQLEMENLINDSQFTESSNKAQSGASNKHAELCMGGPSFASTAENSQGKVDQFLIPELDPTDASSNDSLLEILDNFFNNHENLSALSQGPKQESEYLPPCMQNPEREDKMTEQYLSSDNREVFNTETLIKNFRDLHLSLHETHSTLWKQLNTNHLSTKDSDFQEIMHWLFGEVFNPKTKIPLMGRTQVKILDEVKFSPVQFGVMKLLEEPESLEELSLEIVRIWFQMHSDKWEDGNNNSSCDISNNLTKQDTSISLGKMKGLEAVKSKMPFANDSTHKIINFTHCGAFKKVWKKQFRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3K337</accession>
    <name>E3K337_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_04850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323313.1">
        <property type="nucleotide sequence ID" value="XM_003323265.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K337"/>
    <dbReference type="GeneID" id="10536999"/>
    <dbReference type="KEGG" id="pgr:PGTG_04850"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04850"/>
    <dbReference type="HOGENOM" id="CLU_2293045_0_0_1"/>
    <dbReference type="InParanoid" id="E3K337"/>
    <dbReference type="OrthoDB" id="10274463at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171726" evidence="1">
        <location>
            <begin position="20"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP78894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="101" mass="10921" checksum="F8487BD13CCD5FEF" modified="2011-01-11" version="1">MHLITTTIVILSACGAAFGRLECPRARPHGMCGTPTPDGLSLIMSNLTPTYEADPLVYAGCQTTPYCGDDWMWKRVDAAMGSGEGIPKDATFDQHCQEMPK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3K4D8</accession>
    <name>E3K4D8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ricin B lectin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05737</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EFP79416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323835.1">
        <property type="nucleotide sequence ID" value="XM_003323787.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K4D8"/>
    <dbReference type="GeneID" id="10531240"/>
    <dbReference type="KEGG" id="pgr:PGTG_05737"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05737"/>
    <dbReference type="HOGENOM" id="CLU_111419_0_0_1"/>
    <dbReference type="InParanoid" id="E3K4D8"/>
    <dbReference type="OMA" id="DGYFINA"/>
    <dbReference type="OrthoDB" id="2499440at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.80.10.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035992">
        <property type="entry name" value="Ricin_B-like_lectins"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000772">
        <property type="entry name" value="Ricin_B_lectin"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00652">
        <property type="entry name" value="Ricin_B_lectin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50370">
        <property type="entry name" value="Ricin B-like lectins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50231">
        <property type="entry name" value="RICIN_B_LECTIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ricin B lectin" evidence="1">
        <location>
            <begin position="8"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="199" mass="22472" checksum="FADFE468D60CA84C" modified="2011-01-11" version="1">MADFPTAWFYIKSVCSKKVIQPLGGSFEPTRLVVVDQKFGQESAAQLWKHENGYLVNKLTNLCLDYEHGNYKRLGDIHVCQWHQKVGKDAHNQKWLYRTSNLIASNDDINRVLDIKGGSIHPGAEVLLKKLETIKGAHPAHQRWLLEVIDQDGLPDSLSTYQEDQIAGSYAAPVEHVNPWATLEPSTDDEPGEAQTTYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3K6A5</accession>
    <name>E3K6A5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Cyanovirin-N domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05061</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
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    <feature type="chain" id="PRO_5003173268" description="Cyanovirin-N domain-containing protein" evidence="1">
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            <begin position="25"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
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    </evidence>
    <sequence length="178" mass="18590" checksum="4F68579FC4519633" modified="2011-01-11" version="1">MLQLAASRLIMLFPLLLVALQSSAKPTPTEGLLVCSGSAPVGKIAPSSGAYYAISDTPHNASGKCSCDHSTGRLDCAVAPKFPISTPQGPAVCQSPGKKTCDSSNIFLCQSGKLIGSLNSEGGFSVTGQSDKASGYCQCDARGKEWLLRCPDYPTSSSFSQFTKKHIDCSKKDDCSGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="76" xmlns="https://uniprot.org/uniprot">
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        <recommendedName>
            <fullName evidence="11">HSF-type DNA-binding domain-containing protein</fullName>
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        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="7">Homotrimer. Homotrimerization increases the affinity of HSF1 to DNA. Interacts with transcriptional coregulator SSA1 on chromatin.</text>
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            <location evidence="1">Nucleus</location>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06588"/>
    <dbReference type="eggNOG" id="KOG0627">
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    <dbReference type="GO" id="GO:0005634">
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        <property type="project" value="UniProtKB-SubCell"/>
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        <property type="project" value="InterPro"/>
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        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
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        <property type="entry name" value="HSF_DNA-bd"/>
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        <property type="entry name" value="WH-like_DNA-bd_sf"/>
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    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015:SF427">
        <property type="entry name" value="HEAT SHOCK FACTOR PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10015">
        <property type="entry name" value="HEAT SHOCK TRANSCRIPTION FACTOR"/>
        <property type="match status" value="1"/>
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    <dbReference type="Pfam" id="PF00447">
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
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    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
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            <end position="154"/>
        </location>
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        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
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        <source>
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        </source>
    </evidence>
    <sequence length="842" mass="92345" checksum="9174733BFEB02F1A" modified="2012-04-18" version="2">MSNSPSYPNTNNTVIDPNQPSMAITKRIHPGALTVDKGSSHRNTERPIPAFVTKLFTMVNDPNTDHLIKWSEPNGDSFFVVSSERFGRELLPKFFKHSNFGSFVRQLNMYGFHKVPHLNQGVLQGEIPETEMLEFTNINFQRSQPDLLCLIRRKKPVPESSNPAPNEPTETGSTPLPSNPSVPHTTDLQAILVDILAIRKHQTLMSSDLKTLQSSNAHLWKEAIANRDRIKRCQDTINKILGFLAQVFAGKVSNLEDPPLGGSIPDLDRHRSRAKSSREHHHHPHPNNFNYESSPSNTALSLYSQMRHPRLMLEDTQRPSSNSSTDQSHPLADNVSSFGKSLDGHASLQNLSFNPNNRNHTHSPTPSTHHTSSTTSSPKVGQSSQFQDSSIDPNLETSIDANGTNGQALHNPVSSTNDQYSPDAVLQALFNNNTSTGGFNINDNPFTSINWADLVSSDGLGGFNEDKGEPAMNPPSPLRITDGHSTRPVSRPSSSPMDMNTDSSTQLISNTQRQAHGIESVANKMDSLETAIERLVNNLPDQMEDYLNPSELDKTDQNANPFNASHLSAAEIASDNQADFDADAFLRTLMPPEDRSQAENRQLYENEEERRTEMSVVLPAGIQTRSVSAAANGKQRSDEHPQSQASASMDALDIEKFLDEWTCQDPSRILPGLTPLVSPVPTPRLELNESEARMAGQPGGSTAAFQDPVASSSSVDKPLTTHSIEAPKSSASVAFDLNDDDHLRRIMSSKKSPTEHRHGHSISHPGTAISNPPPHPPRKRLLDDPGPPQSALTPLTSHQLLSNPFNHLPISSQPSSSSAPNMTTTATTTSSPHFIHNKKTRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3K9D8</accession>
    <name>E3K9D8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Ribosomal eL28/Mak16 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_07129</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL28 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP80877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325296.1">
        <property type="nucleotide sequence ID" value="XM_003325248.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K9D8"/>
    <dbReference type="STRING" id="418459.E3K9D8"/>
    <dbReference type="GeneID" id="10539901"/>
    <dbReference type="KEGG" id="pgr:PGTG_07129"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07129"/>
    <dbReference type="eggNOG" id="KOG3412">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_1403069_0_0_1"/>
    <dbReference type="InParanoid" id="E3K9D8"/>
    <dbReference type="OMA" id="GKYGQRP"/>
    <dbReference type="OrthoDB" id="338850at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.110">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002672">
        <property type="entry name" value="Ribosomal_eL28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029004">
        <property type="entry name" value="Ribosomal_eL28/Mak16"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10544">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01778">
        <property type="entry name" value="Ribosomal_L28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal eL28/Mak16" evidence="4">
        <location>
            <begin position="66"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP80877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="194" mass="20879" checksum="D7DDCEE4C87E714D" modified="2011-01-11" version="1">MALQPVKSGFGRCPARCGAAGPPERHCTGWYARLAALASQTWKPTAPDTRIRLRLYQMDNAGSADLQWFLLRGWNSKVVKRGSAGFMFSSESGNLKNKHSPRYSGLVHPKPMAVTPAPSGGVLITTRKASANPRHIKSARSTVRVKGSMAGSRRAAGKAAKAAKNYRSDLARAAILRAARIAQSNIRTNKPKSA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAC2</accession>
    <name>E3KAC2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FunK1 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_06900</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81279.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325698.2">
        <property type="nucleotide sequence ID" value="XM_003325650.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KAC2"/>
    <dbReference type="GeneID" id="10536767"/>
    <dbReference type="KEGG" id="pgr:PGTG_06900"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06900"/>
    <dbReference type="eggNOG" id="ENOG502S5WB">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_005513_5_2_1"/>
    <dbReference type="InParanoid" id="E3KAC2"/>
    <dbReference type="OrthoDB" id="5584477at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040976">
        <property type="entry name" value="Pkinase_fungal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38248:SF2">
        <property type="entry name" value="FUNK1 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38248">
        <property type="entry name" value="FUNK1 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17667">
        <property type="entry name" value="Pkinase_fungal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fungal-type protein kinase" evidence="2">
        <location>
            <begin position="243"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="712"/>
            <end position="750"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="712"/>
            <end position="727"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP81279.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="750" mass="85789" checksum="0A3168ADC1BA4E76" modified="2012-04-18" version="2">MAQESGPASIHEALRSYLTSNGIETVEKFFEGGQDQIKKRLSLLGAEITIQSCLADKLDGYWMTHGTNRISRLVTVDEFKTYEPLITYILLNQAKSNQEMLQLINKEHSEPPTPQPVTSTALVRTGNISGLTNSSTKVSDFVPTLKRELNKLLYTNVQGLVEHFIDPRHIDPTHRKRTNARSRFIDQKRKKKLSNTLQGSMLKYISSFAEHLVNQTKPKNPMSRIWRTQPHTRLEGVDGTRMVDGAIMSGVNANQKNYHIRDVLVPFELKKDKQDLTKAVICLGKYVCEVFKAQPTRSFVIGVTVFETSIQIWQFDRSGAIGSEPVDFKSDKPNIEKFLDLMLCFLTCNKDLLGFDPTFIEAQDRPPVLRIQTQNDVNQELEIEPKPVFRASGICGRGTTCWKAHLSGDKSQQFLIKDSWQPEQRPKEGEMLRIVTEKKVPHVARYHHHEDVCVDGKMVDIKSHVRRGADFQNCEKLTNKEKPDDLNVQNEFINRVHRRLILKDVGKPIWTVDSPLRLLEALESCIIGHQALFAAGYLHRDISINNLMVNDQAVDPLYRSFLIDLDVAIHCSMSSGDDGHTRTGTKVFMSSSLLRGGNPHTPVDDLESFFWVLIWICIHYPKDKRNGISELASWNQQLPKNLAGIKNEYLTTPCDLTKLFTAQYRGKHLSDCVIRFAQLMSNQKIRSWKHQRLYEKILTILQEAQQKLREEETLHKEEKLREEEKKSQTGKKRKKAAENKSCVGKKRIAV</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAC6</accession>
    <name>E3KAC6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_06904</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81283.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325702.2">
        <property type="nucleotide sequence ID" value="XM_003325654.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KAC6"/>
    <dbReference type="GeneID" id="10536771"/>
    <dbReference type="KEGG" id="pgr:PGTG_06904"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06904"/>
    <dbReference type="HOGENOM" id="CLU_1058210_0_0_1"/>
    <dbReference type="InParanoid" id="E3KAC6"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="90"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="214"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP81283.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="263" mass="29733" checksum="96380CB38014EFC9" modified="2012-04-18" version="2">MPMLVQRLDCSSDELPTSIQEVDQEDIKKISTKNKTSFQPQLIRSQPIPTRLNIIIINIKFSLSLISLKPNPSLPIILTLIKQQQQQHPSLAIAILILISLSTLTILIDYWTQSQLYRLRYPWTLSPSKGSSPSTSSWKSLSEGYLGLPACDPFGEPGTVVLNTSFYHDASWVPFGPSCAPSPDYLSALRAIREVPINLPLSQREALIHRPIHPSSSSSKEQEQQEPQQTEFFDRSGFDLWGRPYPDLTFLRGKTILLKRNEH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAK1</accession>
    <name>E3KAK1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Major facilitator superfamily (MFS) profile domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_07039</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81418.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325837.2">
        <property type="nucleotide sequence ID" value="XM_003325789.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KAK1"/>
    <dbReference type="FunCoup" id="E3KAK1">
        <property type="interactions" value="71"/>
    </dbReference>
    <dbReference type="GeneID" id="10537072"/>
    <dbReference type="KEGG" id="pgr:PGTG_07039"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07039"/>
    <dbReference type="HOGENOM" id="CLU_001265_0_1_1"/>
    <dbReference type="InParanoid" id="E3KAK1"/>
    <dbReference type="OrthoDB" id="2502798at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1250.20:FF:001482">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791">
        <property type="entry name" value="PERMEASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43791:SF85">
        <property type="entry name" value="TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_6G00710)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="78"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="171"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="328"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="352"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="379"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="413"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="445"/>
            <end position="466"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="8">
        <location>
            <begin position="45"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="491"/>
            <end position="516"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="491"/>
            <end position="505"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP81418.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="528" mass="58084" checksum="4DE9CDAAE0771D76" modified="2012-04-18" version="2">MESEENRNLQTDSNATHYLKDSSHIPQPSNPSRHKRALLKLDLLLIPVLGLFYFLSFLDRSNLGNVRIIGLQRDLDMTDYQFSMALTVTFIPYILIELPSNLLVRRIGAGILIPAIVTIWGLVTCLQGLVTSYHGLLVSRFFLGLVEGGLYPAIILYLSSFYTRTELQFRIALFWGTICVAGAASGLLTYAIIKLDGQWGHPGWAWVFLIEGFVTAVCGVIGFFILPARIETVRILTEEEKTLLILRLEAASTTGSNAIASDPDLSSHQYPAKSTMTQVWEACKSPHVILLNVAQFACAGNIYSLAYFTPTIVNSFGYSPSATQLFSVPPFGVALIFLLGISYWSDARQARGLACSISAVLSIVGFAIFYASGHEQVRYGSLFLSVPGAYGVSPSLSAWTADNSAPHMRKATALALGTMVGNSGGLFSVWIFVLGHKPRYSLPTAINLFFGVLIIACSILNTLWLSHAQKRKVARRNEILEKFGPLEGGKAFDEAKLEPEDRPSKQDLASAQAWDHLGDQHPDFKYIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBA1</accession>
    <name>E3KBA1_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RFX-type winged-helix domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_07863</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="P:chromatin organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052406">
        <property type="entry name" value="Chromatin_Remodeling_Comp"/>
    </dbReference>
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        <property type="entry name" value="DNA-bd_RFX"/>
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    <dbReference type="PANTHER" id="PTHR22970">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22970:SF14">
        <property type="entry name" value="AT-RICH INTERACTIVE DOMAIN-CONTAINING PROTEIN 2"/>
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    <keyword evidence="1" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
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    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="RFX-type winged-helix" evidence="5">
        <location>
            <begin position="39"/>
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    </feature>
    <feature type="domain" description="RFX-type winged-helix" evidence="5">
        <location>
            <begin position="211"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51526"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP81614.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="481" mass="52675" checksum="9BCBB11EE0BFBCD4" modified="2012-04-18" version="2">MSPLPPTSVQVADTTVTAGDLLVGQHLRRVIRLHEPSRTRAWIATMFEADPDGEIEQVALWGMYRMQFEPHHVEDAVAPIPPLAVATEVITVMESVFRPDSYVAADGQKRYVIRGLKLRPAIERDAVLDDMFRAPDPTPSAPAPSTPHHLANAVNSLNTPSSSAALGNNVTHHQVHQAQPAPPQLPVARSADLLDEADILKIVSFPEPYRTRLWIASLFEPDPTSNAGPGGGGVEQVTLWQLYQKQLTQYVVEGAPSDKFVPPLAPATEVIKAVQDVFPEGRVVVRLEDGKTHYMIIGIKLRPSNFRSPALKSLLSITATKIPNNVTHNGSIGNSSQTDPVAPQLVSKVDSLVKTVEELQNVIKKQESTIKQELAWSATRQINLRTSDSNTSILRPLSIAPPHGAPAFPVDFPKTVEEAQRLDYTQCVALLERYGQYNLIIEGNEERTRTGLLEFIGCVPQELSLAENYVSNVGPNVNSMV</sequence>
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            <fullName evidence="1">Uncharacterized protein</fullName>
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    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <lineage>
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    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178279">
        <property type="protein sequence ID" value="EFP81501.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="E3KBK5"/>
    <dbReference type="GeneID" id="10537239"/>
    <dbReference type="KEGG" id="pgr:PGTG_07750"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07750"/>
    <dbReference type="HOGENOM" id="CLU_1595353_0_0_1"/>
    <dbReference type="InParanoid" id="E3KBK5"/>
    <dbReference type="OrthoDB" id="10297080at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP81501.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="167" mass="18827" checksum="F341383B8D4B45AE" modified="2012-04-18" version="2">MASIHASIPLHMTRQVSFTDTSYTVLYSPPPIPSTLNLESIRETRLCLEIHVDSQSWDILPDPVHRHTLSPIMTGTMCISKVSPRIEGFSLIKGPDFGPTFVLHASPLVNKEHLMGLIPSNVILYSGAIAFEKGRSLHIAAETIELFHNYQNPHTYNLRSRSFYWSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3KBR0</accession>
    <name>E3KBR0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerases I, II, and III subunit RPABC3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_07968</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
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            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively.</text>
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    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
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    <comment type="similarity">
        <text evidence="2 4">Belongs to the eukaryotic RPB8 RNA polymerase subunit family.</text>
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    <dbReference type="eggNOG" id="KOG3400">
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        <property type="component" value="Unassembled WGS sequence"/>
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    <dbReference type="GO" id="GO:0005736">
        <property type="term" value="C:RNA polymerase I complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    <dbReference type="GO" id="GO:0005665">
        <property type="term" value="C:RNA polymerase II, core complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005666">
        <property type="term" value="C:RNA polymerase III complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005570">
        <property type="entry name" value="RPABC3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10917">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10917:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03870">
        <property type="entry name" value="RNA_pol_Rpb8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000779">
        <property type="entry name" value="RNA_pol_Rpb8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00658">
        <property type="entry name" value="RPOL8c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0804">Transcription</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR000779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP81719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="148" mass="16477" checksum="60118A8B60F2B667" modified="2011-01-11" version="1">MADSSLLFDDLFTITNVDKDGKKFDRVSRLNASSTNHSMTLVLDFNVELYPLKVGDSFALALSSSLTPEGEKDSGIETQSWRLEEGKGGLADEYEYVMYGKLYKFDSGASDSVTAYISFGGLLMALTGDYRHLDRLTVGQMVFLLMRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>E3KD89</accession>
    <name>E3KD89_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutamate pyruvate transaminase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glutamic--alanine transaminase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Glutamic--pyruvic transaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_07510</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.</text>
    </comment>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82113.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326532.2">
        <property type="nucleotide sequence ID" value="XM_003326484.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KD89"/>
    <dbReference type="FunCoup" id="E3KD89">
        <property type="interactions" value="182"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KD89"/>
    <dbReference type="GeneID" id="10534644"/>
    <dbReference type="KEGG" id="pgr:PGTG_07510"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07510"/>
    <dbReference type="eggNOG" id="KOG0258">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_014254_3_0_1"/>
    <dbReference type="InParanoid" id="E3KD89"/>
    <dbReference type="OrthoDB" id="1732682at2759"/>
    <dbReference type="UniPathway" id="UPA00528">
        <property type="reaction ID" value="UER00586"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042853">
        <property type="term" value="P:L-alanine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1970:FF:000001">
        <property type="entry name" value="Alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000012">
        <property type="entry name" value="alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1150.10:FF:000010">
        <property type="entry name" value="Alanine aminotransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045088">
        <property type="entry name" value="ALAT1/2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751">
        <property type="entry name" value="ALANINE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11751:SF29">
        <property type="entry name" value="ALANINE TRANSAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="10">
        <location>
            <begin position="143"/>
            <end position="510"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00077894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00078532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00080525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP82113.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="534" mass="58825" checksum="510D020BC9808F82" modified="2012-04-18" version="2">MSMIVNWSKQLISSTATATATATTISRRTTAIRTTNSRYSTTTTTTRRSSKNQTMISVSDLNPAIIQAQYAVRGRIALRAEQLRATLQADPDAKNQLGFDSIINCNIGNPQQLGQKPITFYRQVACLTEYPELMDAPEASKLFPKDVVDRARSLLDSIGSVGAYSHSMGVPIIRQHIAQFIQKRDGFPADPETIYLTAGASAGVSNIMQLLLSKETDGVMIPIPQYPLYTAALALNSARAVEYYLSEADDWAPNLAGLEEVIRKAQEEDQTKVRAMVVISPGNPVGNCLSQESMEAIVRFCFKHKILLLADEVYQTNIFEPEQRPFVSFKKVVRGMEESIASGQGLISFHSISKGQTGECGRRGGYFELVNIPKEVQEQVYKMASIQLCPPLAGQIGVDLQVKPPLPGDESYPLFKSEVDSIAQALADRSKTLADSFNRLDGLSCRKAQGAMYLFPKLELPTKAHQAAEQAGLPVDEFYCMELLNQTGICIIPGSGFGQEPGTFHFRTTFLAPQVEDYVARFKQFHSAFLNTYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDB5</accession>
    <name>E3KDB5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">18S rRNA (guanine(1575)-N(7))-methyltransferase Bud23 C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_07527</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the class I-like SAM-binding methyltransferase superfamily. BUD23/WBSCR22 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82130.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326549.2">
        <property type="nucleotide sequence ID" value="XM_003326501.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDB5"/>
    <dbReference type="FunCoup" id="E3KDB5">
        <property type="interactions" value="557"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KDB5"/>
    <dbReference type="GeneID" id="10534661"/>
    <dbReference type="KEGG" id="pgr:PGTG_07527"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07527"/>
    <dbReference type="HOGENOM" id="CLU_055194_0_2_1"/>
    <dbReference type="InParanoid" id="E3KDB5"/>
    <dbReference type="OrthoDB" id="2877at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016435">
        <property type="term" value="F:rRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070476">
        <property type="term" value="P:rRNA (guanine-N7)-methylation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000017">
        <property type="entry name" value="probable 18S rRNA (Guanine-N(7))-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039769">
        <property type="entry name" value="Bud23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022238">
        <property type="entry name" value="Bud23_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12734:SF0">
        <property type="entry name" value="18S RRNA (GUANINE-N(7))-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12734">
        <property type="entry name" value="METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12589">
        <property type="entry name" value="WBS_methylT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-0539">Nucleus</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="10">
        <location>
            <begin position="62"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="18S rRNA (guanine(1575)-N(7))-methyltransferase Bud23 C-terminal" evidence="11">
        <location>
            <begin position="208"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="215"/>
            <end position="259"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="274"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="215"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="9">
        <location>
            <begin position="239"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="9">
        <location>
            <begin position="277"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF12589"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP82130.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="14">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="296" mass="33215" checksum="29EF0487DD984910" modified="2012-04-18" version="2">MSRPEHQAPPEIFYNDNEAEKYTKSSRIQSIQSEMTERCLELLNLPSQSDDPGDHDYSALLLDIGCGSGLSGEIISDHGHQWVGVDISGSMLEIALDREVDGDLCLHDIGQGFGFRPGSFDGAISVSVIQWLCNADKSSNSPPKRLKAFFETLFGALVRGARAVFQFYPESDDQVRFIMSFANRAGFTGGLVVDYPNSQKAKKFYLVLMTGPSPHSTTTNNANQLPRGLTTEDQNMRVKYSKKSNHKKNKRSSTKGAIDESKQAWIYKKKELYRKRGKEDVPRDSKFTGRKRKTQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDJ1</accession>
    <name>E3KDJ1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08383</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82427.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326846.2">
        <property type="nucleotide sequence ID" value="XM_003326798.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDJ1"/>
    <dbReference type="GeneID" id="10529806"/>
    <dbReference type="KEGG" id="pgr:PGTG_08383"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08383"/>
    <dbReference type="HOGENOM" id="CLU_1876458_0_0_1"/>
    <dbReference type="InParanoid" id="E3KDJ1"/>
    <dbReference type="OrthoDB" id="2506873at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82427.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="136" mass="15190" checksum="CC6B121F3ECF9BD1" modified="2012-04-18" version="2">MRMVAAYNYIHGLVEKVARRNGSTTKKAGRAASTDPAAMKSSRPHELPLAFGDFFVREQRSANGRSCNLLADRPSKYTSYSLSEGFQRESEKIYLANLLRSAAWKLDSRLIPVLALFYFASELGRLSLNNIRSIGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KDP0</accession>
    <name>E3KDP0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08432</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326895.1">
        <property type="nucleotide sequence ID" value="XM_003326847.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KDP0"/>
    <dbReference type="GeneID" id="10529855"/>
    <dbReference type="KEGG" id="pgr:PGTG_08432"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08432"/>
    <dbReference type="HOGENOM" id="CLU_108601_0_1_1"/>
    <dbReference type="InParanoid" id="E3KDP0"/>
    <dbReference type="OrthoDB" id="10273218at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007612">
        <property type="entry name" value="LOR"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04525">
        <property type="entry name" value="LOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173474" evidence="1">
        <location>
            <begin position="23"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="219" mass="24211" checksum="184B3F6C9BDE8FA8" modified="2011-01-11" version="1">MHFLATFVNALSCLSFATQTVASPHQADPAALSNIFIMRQAMDYSQGALPIYASNGTIAFRFIRSIHDPLTGSTTILLKDLASGTLNVVESSNSLCAHKTTYSDMEVPVSQGRQFTIDPNGLLKDQWSFSYLDVTGTQQNFKFDRNYASKEGEIYHQFKGKNGALVAELRNQKRTDAWITTPSVHEVDTYTLSCADNSPQVDLVFLMGLVMTRVHDCGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3KET2</accession>
    <name>E3KET2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">FAD synthase</fullName>
            <ecNumber evidence="2">2.7.7.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">FAD pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">FMN adenylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">PGTG_08982</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="15" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>FMN + ATP + H(+) = FAD + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17237"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.7.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.2" evidence="2"/>
    <dbReference type="EMBL" id="DS178283">
        <property type="protein sequence ID" value="EFP82786.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327205.2">
        <property type="nucleotide sequence ID" value="XM_003327157.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KET2"/>
    <dbReference type="FunCoup" id="E3KET2">
        <property type="interactions" value="131"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KET2"/>
    <dbReference type="GeneID" id="10530169"/>
    <dbReference type="KEGG" id="pgr:PGTG_08982"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08982"/>
    <dbReference type="HOGENOM" id="CLU_056971_0_1_1"/>
    <dbReference type="InParanoid" id="E3KET2"/>
    <dbReference type="OrthoDB" id="270728at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003919">
        <property type="term" value="F:FMN adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006747">
        <property type="term" value="P:FAD biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd23948">
        <property type="entry name" value="FAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000347">
        <property type="entry name" value="FMN adenylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23293:SF9">
        <property type="entry name" value="FAD SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23293">
        <property type="entry name" value="FAD SYNTHETASE-RELATED FMN ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoadenosine phosphosulphate reductase" evidence="13">
        <location>
            <begin position="55"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoadenosine phosphosulphate reductase" evidence="13">
        <location>
            <begin position="142"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012393"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="EFP82786.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="244" mass="27190" checksum="24A7C42D0A79D7D6" modified="2012-04-18" version="2">MANPDSLRSRFGKSDADRVYQLAHSPTPTQLSLQIKHALEIIELAIVRFGVDGLSISFNGGKDSTVLVHLFAAALYRNSSIPTSSEPEPNLKISGLYIRCQSPFAEVDEFVNHCQTIYNIDLMTVDNSLKNGLGAFLAERPSIKAILIGTRATDPNGGSLTDFDPTDSDWPSIIRVHPILDWGYSHVWEFLQKLEVDWCKLYNSGYTSLGSSFNTFKNPFLETDDGWKAAWELSDGRNERAGRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3KEZ0</accession>
    <name>E3KEZ0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09810</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178284">
        <property type="protein sequence ID" value="EFP82842.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327261.2">
        <property type="nucleotide sequence ID" value="XM_003327213.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KEZ0"/>
    <dbReference type="GeneID" id="10544552"/>
    <dbReference type="KEGG" id="pgr:PGTG_09810"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09810"/>
    <dbReference type="HOGENOM" id="CLU_073145_1_0_1"/>
    <dbReference type="InParanoid" id="E3KEZ0"/>
    <dbReference type="OrthoDB" id="2503796at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="1"/>
            <end position="11"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82842.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="209" mass="22843" checksum="3DB543968A90D3AF" modified="2012-04-18" version="2">MLALHRPKTHKPSSPPNQSQGQLFQPLTNAEELEQAWKVSTNTVGSSYNLYKVTELSDQKDKFGRCSTKINQPMSDSSCGNLNKQAAICLGKQQEGSKTCNLASVGITGTGDIDPKEVPQLCAVWCAEATCPFYTLVDASHKAILHPAVLKHLPTRKAVSKDIHLLYSAIQDSYRTILKEQQGALYLSVDTCQSPNGFESFGHSHLPIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGC4</accession>
    <name>E3KGC4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_08535</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83349.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327768.2">
        <property type="nucleotide sequence ID" value="XM_003327720.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KGC4"/>
    <dbReference type="GeneID" id="10545065"/>
    <dbReference type="KEGG" id="pgr:PGTG_08535"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08535"/>
    <dbReference type="HOGENOM" id="CLU_2251398_0_0_1"/>
    <dbReference type="InParanoid" id="E3KGC4"/>
    <dbReference type="OrthoDB" id="10275747at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171981" evidence="2">
        <location>
            <begin position="24"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="30"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="66"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP83349.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="104" mass="11638" checksum="004A5D399B9A4E08" modified="2012-04-18" version="2">MFQKNFLALFIIFEVLQIFPSLALPSNEITKGVSATTETSRAGSRSQSTSMQVESVQRMVHPDGQNPKRELTESEKVNQGQKSLCGSILHFACTPLTCDWFEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGQ3</accession>
    <name>E3KGQ3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">CFEM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">PGTG_08664</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="18" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Lipid-anchor</topology>
            <topology evidence="1">GPI-anchor</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Secreted</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the RBT5 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327897.1">
        <property type="nucleotide sequence ID" value="XM_003327849.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KGQ3"/>
    <dbReference type="GeneID" id="10532155"/>
    <dbReference type="KEGG" id="pgr:PGTG_08664"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08664"/>
    <dbReference type="eggNOG" id="ENOG502SFDE">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_063084_3_2_1"/>
    <dbReference type="InParanoid" id="E3KGQ3"/>
    <dbReference type="OMA" id="NSISCCV"/>
    <dbReference type="OrthoDB" id="2507492at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009277">
        <property type="term" value="C:fungal-type cell wall"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051735">
        <property type="entry name" value="CFEM_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008427">
        <property type="entry name" value="Extracellular_membr_CFEM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37928">
        <property type="entry name" value="CFEM DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G14090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37928:SF2">
        <property type="entry name" value="GPI ANCHORED CFEM DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G10580)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05730">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00747">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52012">
        <property type="entry name" value="CFEM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-1015">Disulfide bond</keyword>
    <keyword evidence="12" id="KW-0325">Glycoprotein</keyword>
    <keyword evidence="6" id="KW-0349">Heme</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="13" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="10" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0964">Secreted</keyword>
    <keyword evidence="8 15" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="15">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173569" description="CFEM domain-containing protein" evidence="15">
        <location>
            <begin position="26"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="CFEM" evidence="16">
        <location>
            <begin position="6"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="14">
        <location>
            <begin position="108"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="14">
        <location>
            <begin position="108"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022525"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS52012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="EFP83478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="161" mass="15908" checksum="CCC3CF25A98C124D" modified="2011-01-11" version="1">MLSFTALPFLFSLGTVLVSVPATGAQDISGLPPCASNCLAVSLTSTAKSCAPTDFACLCKNSEFISASSSCYSKSCSAGDLASAQAWGIKSCAAAGVQIAVDGLSNATSPGTNTSTSTSSNSSPLNNTSTSARPNSAGALPVETGMSFAFGSALVVSLLML</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3KH53</accession>
    <name>E3KH53_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Eisosome component PIL1-domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_09341</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328047.1">
        <property type="nucleotide sequence ID" value="XM_003327999.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KH53"/>
    <dbReference type="FunCoup" id="E3KH53">
        <property type="interactions" value="20"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KH53"/>
    <dbReference type="GeneID" id="10532314"/>
    <dbReference type="KEGG" id="pgr:PGTG_09341"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09341"/>
    <dbReference type="eggNOG" id="ENOG502QQ1T">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_465501_0_0_1"/>
    <dbReference type="InParanoid" id="E3KH53"/>
    <dbReference type="OMA" id="HHQLRSF"/>
    <dbReference type="OrthoDB" id="5599269at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036286">
        <property type="term" value="C:eisosome filament"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008289">
        <property type="term" value="F:lipid binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070941">
        <property type="term" value="P:eisosome assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006897">
        <property type="term" value="P:endocytosis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.60">
        <property type="entry name" value="Arfaptin homology (AH) domain/BAR domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027267">
        <property type="entry name" value="AH/BAR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028245">
        <property type="entry name" value="PIL1/LSP1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31962:SF6">
        <property type="entry name" value="EISOSOME COMPONENT PIL1-DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31962">
        <property type="entry name" value="SPHINGOLIPID LONG CHAIN BASE-RESPONSIVE PROTEIN PIL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13805">
        <property type="entry name" value="Pil1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="249"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="412"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="558"/>
            <end position="586"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="140"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="255"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="301"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="362"/>
            <end position="375"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="413"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="451"/>
            <end position="469"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="504"/>
            <end position="521"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="530"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="558"/>
            <end position="568"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP83628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="586" mass="62763" checksum="56B2E7BBD4BD7EAA" modified="2011-01-11" version="1">MSSFLSDAKARIQHTNKLSLAPKDIKNLAEIISTEKSVLSTSSKLSVDYRKAAEALKEWGLNEGDDLADILPKLSILLGHLADAQSRFSDHDGTYRIHFKSIRMREESLAALKKSKETIHAKITGLEKKITKMSSENKDLPALTTRLQEARSELISLENSVAIEDARLSDFKRETVREGLGLRLGAMLELAEKMTIVCEFGKMLTNEVPIERTPPGAARAPYCAANKTQAVVEEAQRCLTQVVFNPSHSAGPLSIENTSEVPPSQSPTSESEHPIPPHQAPHHSDASNSLPMELPPLYFSQAATNDPAKSSSQADANPNGSSYNQATHAPLDNYSSLPYTQESSEPSAHPTSNPHYSHNTDSTSSPPLPSSSQSPATMASRLASRGPADTAGESHERPSYIENVFYVRGLNGVPQSTGPGNPTEAVNSPSSIYSPPPSANPPSEGQARLSRYSQPSVQNSPYQYTSSLASADVPPKTSSVVGRSETDEPVTKSRYSPRPRDHSISSGVSGVPTSDGRTTNAGAFRRAAGNQLSSPPSSQARSIGSTQAFIATDEALTTANSSTVQPLQINKRDTAGSGDLSTRRNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHB9</accession>
    <name>E3KHB9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_09407</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83694.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328113.2">
        <property type="nucleotide sequence ID" value="XM_003328065.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHB9"/>
    <dbReference type="GeneID" id="10532380"/>
    <dbReference type="KEGG" id="pgr:PGTG_09407"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09407"/>
    <dbReference type="HOGENOM" id="CLU_2211220_0_0_1"/>
    <dbReference type="InParanoid" id="E3KHB9"/>
    <dbReference type="OrthoDB" id="10269155at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP83694.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="107" mass="11881" checksum="F67BE776F10DC040" modified="2012-04-18" version="2">MCDLFFYEAEGTVVRPPFYDLPTLRPKTLKGNQCAVQLPHRASSATLKGTRLLLLMQAIRNTSPTKPDGVSCVYGHARRPVTVPASHRFMCLCSRKPPELRGLTAIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHL1</accession>
    <name>E3KHL1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09499</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83786.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328205.2">
        <property type="nucleotide sequence ID" value="XM_003328157.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHL1"/>
    <dbReference type="GeneID" id="10532473"/>
    <dbReference type="KEGG" id="pgr:PGTG_09499"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09499"/>
    <dbReference type="HOGENOM" id="CLU_044116_0_0_1"/>
    <dbReference type="InParanoid" id="E3KHL1"/>
    <dbReference type="OrthoDB" id="10291445at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="295"/>
            <end position="317"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83786.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="412" mass="45455" checksum="5B3B1A89FC52A748" modified="2012-04-18" version="2">MALPRPEDSGVPDAVELELEAYNAQLENPPRQCPINLDYILYLQRLAIDRPAMGCPNSLTWEEIGPEPLPTPMILDLQSMTWKRFQALVLAHLCKDSPELHKFLVGRNHAQEIVWLASINGHRFYAVSCEVPIKGSLDFLQFAAAAYKAYPSAVLFKLVMKDPSVTHGPKAPISPKEQVLRSLNNIVGCILSGNTYSAGATTILINPDDPRRRMQISLAAVIDWAQAIRLKNPGVDYTHPPDNANYRWVDWTSRKRAASSQSIPPSKRNLMDASAIMSMAARVKSVGFNKPGADTAADAGADTATDEISSTRKTPLPPASAALEKHHMETYLRVSHIGQDDQLTRARLLLHGITHWTFFRSSSEPELTQLGFPLGIARLLCEGVGRLERYEADMSRFSTLSAAGPSGSQMDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHU4</accession>
    <name>E3KHU4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Piwi domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09582</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EFP83869.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328288.2">
        <property type="nucleotide sequence ID" value="XM_003328240.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHU4"/>
    <dbReference type="STRING" id="418459.E3KHU4"/>
    <dbReference type="GeneID" id="10532558"/>
    <dbReference type="KEGG" id="pgr:PGTG_09582"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09582"/>
    <dbReference type="HOGENOM" id="CLU_1611587_0_0_1"/>
    <dbReference type="InParanoid" id="E3KHU4"/>
    <dbReference type="OrthoDB" id="10252740at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003165">
        <property type="entry name" value="Piwi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22891">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02171">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00950">
        <property type="entry name" value="Piwi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50822">
        <property type="entry name" value="PIWI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Piwi" evidence="1">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83869.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="165" mass="18516" checksum="C04E0EC985C88963" modified="2012-04-18" version="2">MVMGADVTHPGRDSLEPSIAAVVGSTNRYGSCYAAEFSVQPGRQEIISDLHIMVKQLLIKFYQHNRLLPENLIFYRDGVSEGQFPDVVAKEIPLVRQAINAMGEDERCKLTYIICGKRHHFKFGPTTKDKNHCDSKGNLLPGVVIDTSWWNPWNFSSFPLHCPGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3KI84</accession>
    <name>E3KI84_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">BHLH domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_09722</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EFP84009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328428.1">
        <property type="nucleotide sequence ID" value="XM_003328380.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KI84"/>
    <dbReference type="STRING" id="418459.E3KI84"/>
    <dbReference type="GeneID" id="10532704"/>
    <dbReference type="KEGG" id="pgr:PGTG_09722"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09722"/>
    <dbReference type="eggNOG" id="KOG1318">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_578895_0_0_1"/>
    <dbReference type="InParanoid" id="E3KI84"/>
    <dbReference type="OMA" id="THELFND"/>
    <dbReference type="OrthoDB" id="690068at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000981">
        <property type="term" value="F:DNA-binding transcription factor activity, RNA polymerase II-specific"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000978">
        <property type="term" value="F:RNA polymerase II cis-regulatory region sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.280.10">
        <property type="entry name" value="Helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011598">
        <property type="entry name" value="bHLH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036638">
        <property type="entry name" value="HLH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051732">
        <property type="entry name" value="USF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46117">
        <property type="entry name" value="FI24210P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46117:SF3">
        <property type="entry name" value="FI24210P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00010">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00353">
        <property type="entry name" value="HLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47459">
        <property type="entry name" value="HLH, helix-loop-helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50888">
        <property type="entry name" value="BHLH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="BHLH" evidence="6">
        <location>
            <begin position="322"/>
            <end position="406"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="123"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="247"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="421"/>
            <end position="474"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="524"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="25"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="123"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="171"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="421"/>
            <end position="448"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP84009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="583" mass="65171" checksum="B1C0A61B3AA46441" modified="2011-01-11" version="1">MSSHPQSVYGHQQHSIEPKRFNGFFSPSATSNNTNTAPSLINPDHFQFNSLFGSHPAQQQPQFNLSSSSSGLQFTDDELIESLIHRQPINNGNHLGEFPHSPQSSSLTQPISFDQITLLDHPAHRHSQPHSHSNPAHHHPIPITTRTAATSPDSDRRRSFSSSVPIEDQQLLLSPSSTNPARSPSTMSSVSRTTANHQRTFRASRSPNPKPLLNRHPQQAAYPFSPPTSITTTLVDEERSPAISTISVTESSTHHHLSSSQPIGSSFYRPLPSADDATGLPSPLDRMSDELSLSSSLPTRRTFSPHSIIDQQKLVLNEKRRKRRESHNAVERRRRDNINDRITELAGLLPTCLLDTVVTPNESGMMMVVNEEPSQADDSQGLNSASHTKPNKGMILAKSVDYIKHLKHLLELQSQRNTELEAELKRVRSGTEPHQEDPKQQGTEEKVRGGVTGEDEENVKGEEGPGPGGMNEDTSDQIHQWIKIHDQQNDPQNQNESQLEDENMMMTNGFGQDAVSYRPDHNMAHRHHLSSSSDPTNHHHAHNLSTSEPIKFHEDHYSSSLDHFASPFNHRLGSFENIYYHLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIJ2</accession>
    <name>E3KIJ2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">Translation machinery-associated protein 22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_10495</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2 4">Interacts with the 40S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="4">The SUI1 domain may be involved in RNA binding.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the DENR family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328536.1">
        <property type="nucleotide sequence ID" value="XM_003328488.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIJ2"/>
    <dbReference type="FunCoup" id="E3KIJ2">
        <property type="interactions" value="674"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KIJ2"/>
    <dbReference type="GeneID" id="10545215"/>
    <dbReference type="KEGG" id="pgr:PGTG_10495"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10495"/>
    <dbReference type="eggNOG" id="KOG3239">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_073511_0_1_1"/>
    <dbReference type="InParanoid" id="E3KIJ2"/>
    <dbReference type="OMA" id="EVFEIDM"/>
    <dbReference type="OrthoDB" id="277199at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11607">
        <property type="entry name" value="DENR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.780.10:FF:000055">
        <property type="entry name" value="Translation machinery-associated protein 22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050318">
        <property type="entry name" value="DENR/SUI1_TIF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046447">
        <property type="entry name" value="DENR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005873">
        <property type="entry name" value="DENR_eukaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048517">
        <property type="entry name" value="DENR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01159">
        <property type="entry name" value="DRP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789:SF0">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21023">
        <property type="entry name" value="DENR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="SUI1" evidence="7">
        <location>
            <begin position="114"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="78"/>
            <end position="106"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP84117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="214" mass="23921" checksum="DCEB3BF46415263A" modified="2011-01-11" version="1">MASTTEQQDQPTTTTTTTSLQPIQPIKPLYCQICSFPPEYCEYGTSISKCKTWLSTHDQKRYEEIYGGIEQELASMSVEEVEKKKKAETEALKKEAKEDAKASRIKEKSKTNKVTVKTVERTKRKRITTIHGLDLFGVDLKKLAKLFASKFATGSSVTKNNQGEDEIVIQGDVSDEVLDLFDSTTGKFAEIIGNGIIPDDNIVFVDDTKKKKPT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIX7</accession>
    <name>E3KIX7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RNase H type-1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_10630</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328671.1">
        <property type="nucleotide sequence ID" value="XM_003328623.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIX7"/>
    <dbReference type="STRING" id="418459.E3KIX7"/>
    <dbReference type="GeneID" id="10545513"/>
    <dbReference type="KEGG" id="pgr:PGTG_10630"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10630"/>
    <dbReference type="HOGENOM" id="CLU_2265047_0_0_1"/>
    <dbReference type="InParanoid" id="E3KIX7"/>
    <dbReference type="OrthoDB" id="3230070at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP84252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="103" mass="11181" checksum="9B6098D419CDEC68" modified="2011-01-11" version="1">MGTNSTNLAVSNHEAEAVSLIAATSLAGKLCQGRMIRKIIFLVNNIAKGSSKGLKTQLLRNRVDHFFQVIDKNLAALPPWAEVVIVWCPGHKDITGNEMGRLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3KK83</accession>
    <name>E3KK83_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10867</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84708.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329127.2">
        <property type="nucleotide sequence ID" value="XM_003329079.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KK83"/>
    <dbReference type="GeneID" id="10526675"/>
    <dbReference type="KEGG" id="pgr:PGTG_10867"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10867"/>
    <dbReference type="HOGENOM" id="CLU_652344_0_0_1"/>
    <dbReference type="InParanoid" id="E3KK83"/>
    <dbReference type="OrthoDB" id="2507299at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00084">
        <property type="entry name" value="HMG-box_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.30.10">
        <property type="entry name" value="High mobility group box domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036910">
        <property type="entry name" value="HMG_box_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47095">
        <property type="entry name" value="HMG-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="38"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="314"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="57"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="77"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="105"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="136"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="318"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84708.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="421" mass="47363" checksum="F150CE1C0665B335" modified="2012-04-18" version="2">MAHNYGTFSGYVDPQPYPNNNCDHQLMDAEFQTNESLQNSFSGVNNSGFGPGDPNVLFNQMASNSTLAPDPDQMNHTATQGQQNPTGGGTTSQTSTTTSSANARPFKKRKPRKKKSDIPAQVSENSTSNEGREGRNPTTNTTASDPHPSSNRPRGEATTFQNEDNPNLSVSRNRPSLPNNLMSSLQNETLLGLRIAQNTVGQNKRITNQIKDQLKEITLEYQRKIHLLALEHKIRSELLFKWVGVWNKVRGPNRFNNFCRYAAEARKLFDSKLLPPAERMQQVAERWRQLDEDEQLKYNDWDFINSLREKMGLKPVEDPEEIEDEDQEQARELEQDTTGGPKKTDAQVLSHCKAWAKKAVTDMNFFSTQYSVESFLVLASTNINGRVFITGSSFLGADYMQLLSTKAKKGMISSELARTYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLU2</accession>
    <name>E3KLU2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11460</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178294">
        <property type="protein sequence ID" value="EFP85291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329710.1">
        <property type="nucleotide sequence ID" value="XM_003329662.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLU2"/>
    <dbReference type="GeneID" id="10543834"/>
    <dbReference type="KEGG" id="pgr:PGTG_11460"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11460"/>
    <dbReference type="HOGENOM" id="CLU_1171116_0_0_1"/>
    <dbReference type="InParanoid" id="E3KLU2"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="30"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="97"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="36"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="118"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="133"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="157"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="237" mass="24626" checksum="DD620CDA4E5ABE28" modified="2011-01-11" version="1">MGRLLLALVTGGSGSVGTGSVGTGSVGAARAKELSGSGQVHQQTRNQNQLHQNTKNQSSLHQHQNPHQIHNQKNQITQIHRTQRALVGLHPLPLKPASAPFGLPHTSHSHSHIPPQHQDSRSHPHQEPVPDPGTTQAALDPLSSQKNHALINPPLDPSTLSSSPSASGADTDPSLDLGPSSSSSKNLGPLNRPPHGIPIGPHASPQKGLQSPIIVDSSSELTSTLPTTTTTTASSSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KM45</accession>
    <name>E3KM45_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_11563</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178294">
        <property type="protein sequence ID" value="EFP85394.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329813.2">
        <property type="nucleotide sequence ID" value="XM_003329765.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KM45"/>
    <dbReference type="GeneID" id="10543943"/>
    <dbReference type="KEGG" id="pgr:PGTG_11563"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11563"/>
    <dbReference type="HOGENOM" id="CLU_1210337_0_0_1"/>
    <dbReference type="InParanoid" id="E3KM45"/>
    <dbReference type="OrthoDB" id="2518872at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP85394.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="229" mass="26581" checksum="BA1BFC0F640EA816" modified="2012-04-18" version="2">MEDMEGYEKKDWAELKKEMLEKWGQSEQRYWEADLDRLVEEMAEKGGIQMPEQYGKYVLSFDRILKYLNWNKMVTNTLEAEMRDEAVCQLFERNLVKRLKDGSVIVLPEMKEILKALLCEMDMHWDRFGQKLPLRRWRWWQGPLTCSYCGVLGHFKAQWKELTTDIKTSGVCPVIRDYYLDGKKIEAAVPGDKVCKRRSTKPSATAKGKEAVSVGDAKSWSLLATPGAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KM80</accession>
    <name>E3KM80_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11761</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178295">
        <property type="protein sequence ID" value="EFP85405.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329824.2">
        <property type="nucleotide sequence ID" value="XM_003329776.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KM80"/>
    <dbReference type="GeneID" id="10543956"/>
    <dbReference type="KEGG" id="pgr:PGTG_11761"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11761"/>
    <dbReference type="HOGENOM" id="CLU_144321_0_0_1"/>
    <dbReference type="InParanoid" id="E3KM80"/>
    <dbReference type="OrthoDB" id="2516736at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173527" evidence="1">
        <location>
            <begin position="23"/>
            <end position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85405.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="118" mass="10823" checksum="4715589D3A1D9904" modified="2012-04-18" version="2">MKFTFGLLSVVATILSTETVTAVPTHGLHRGYLGGYGGYGGGYGGAYGAGYGGPYGGYGRGYGGGYGGGYGGGYGGGYGGGYGGGYGGGYGGGYGGGYGGGGGLGLGFGLGVGVGVGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>E3KNC5</accession>
    <name>E3KNC5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA 3'-5' helicase</fullName>
            <ecNumber evidence="7">5.6.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_11090</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the helicase family. RecQ subfamily.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.4" evidence="7"/>
    <dbReference type="EMBL" id="DS178296">
        <property type="protein sequence ID" value="EFP85761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330180.1">
        <property type="nucleotide sequence ID" value="XM_003330132.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KNC5"/>
    <dbReference type="STRING" id="418459.E3KNC5"/>
    <dbReference type="GeneID" id="10544334"/>
    <dbReference type="KEGG" id="pgr:PGTG_11090"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11090"/>
    <dbReference type="eggNOG" id="KOG0351">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_011478_1_1_1"/>
    <dbReference type="InParanoid" id="E3KNC5"/>
    <dbReference type="OMA" id="FRAADRY"/>
    <dbReference type="OrthoDB" id="2498827at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd17920">
        <property type="entry name" value="DEXHc_RecQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710:SF105">
        <property type="entry name" value="ATP-DEPENDENT DNA HELICASE Q1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13710">
        <property type="entry name" value="DNA HELICASE RECQ FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="9">
        <location>
            <begin position="59"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="643"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="643"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="8">
        <location>
            <begin position="705"/>
            <end position="723"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP85761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="723" mass="82146" checksum="1DB4134442542F03" modified="2011-01-11" version="1">MPARRRNKKKKARRVRINRITLSENVLKMDEKKLREHIAKEAELFYKGQTPKPLQLDAVMNLTELKHTFVMAGTGFGKSRIAEMYFNLFTAKDGVVLVINPLDALGDNQVAEKKAQGFTAINLAKMTFNKSLSDKILAGAFNFVYLSPETFVDNKLFVSLYYKPEFQARLALIVIDEAHIIYSWGLVASGEAKKSSAHLRTHDVSIFRPSYGNMGAQLMANEGVPILFLSATCRPEARKAIFECLKLDPDNVTIIHGELTRPEIRILRFPMDCSLKKASDLLQIFGQREDIPDSDLLPTLIYLGTRNATGQILKVVNEARGTVGGENNPRSKLIRRYHAVTGNRDKIDTINGFEAAEFPCISCTMALGLGQNWSRVRRVIHLGRADPSNICQMIGRCGRDGKPGLAVLFMERRRKGGKNKADDFLASDKKTDDLRMDALAVTPVCLRICFSLDNSLGYIPRSLEDPSVSEERLQEESEGFPPCKCSNCEPREAELLRKHMAEMNLANFLDMCENAKDLEDIEYTIKKKKKAQAPKKIKMGPMLNALSDTLLKDFTRFFETTYPESSSFVSEDMFDREESDSIAQNLADIHDSGSIAKYIGGEKTAGQLEILHDSIIQFKEGSVYQKHISDVFNYTQHVLSKKQRLESEMEQKKIDAEARKQRDVEERQVIAARKKEASDREKDLKEQEKAAARELKTQEKERKAREKKRRMRKKKNKKNKKRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPQ6</accession>
    <name>E3KPQ6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Replication factor C subunit 5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_12247</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the activator 1 small subunits family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178299">
        <property type="protein sequence ID" value="EFP86291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330710.1">
        <property type="nucleotide sequence ID" value="XM_003330662.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KPQ6"/>
    <dbReference type="FunCoup" id="E3KPQ6">
        <property type="interactions" value="537"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KPQ6"/>
    <dbReference type="GeneID" id="10540304"/>
    <dbReference type="KEGG" id="pgr:PGTG_12247"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12247"/>
    <dbReference type="eggNOG" id="KOG2035">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_042324_5_0_1"/>
    <dbReference type="InParanoid" id="E3KPQ6"/>
    <dbReference type="OMA" id="LKADIMH"/>
    <dbReference type="OrthoDB" id="761538at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031390">
        <property type="term" value="C:Ctf18 RFC-like complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031391">
        <property type="term" value="C:Elg1 RFC-like complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031389">
        <property type="term" value="C:Rad17 RFC-like complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000002">
        <property type="entry name" value="Replication factor C subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000229">
        <property type="entry name" value="Replication factor C subunit 3/5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000136">
        <property type="entry name" value="Replication factor C subunit 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF1">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13177">
        <property type="entry name" value="DNA_pol3_delta2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22534">
        <property type="entry name" value="RFC_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0539">Nucleus</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005378"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00070185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP86291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="360" mass="40249" checksum="A9AE71C5468D7991" modified="2011-01-11" version="1">MSLLVDKYRPKKLEELDYHQGLSDRIRALARTADFPHCLFYGPSGAGKKTRIAATLTELFGPGAQKLRIDQKVFVTPSRRRLDVQVVQSNYHLELTPSDVGQWDRSVVQDVLKEVGQSAQLDSGATQRFKVVVIHGADELTNDAQAALRRTMERHTSTMRLILCATSTAKIIGPIRSRCLLLRVGAPNPEQIAQVLNNVSNKASFSTSLPDETAMAIALSSNGNLRRALLTLDAVRAQDETFSKSRTSPDSIPRPDWEVYIDKLAGVIAKEQSPDKLLEARAMLYELLVHLIPPSVVIVQLAKGLLTRVDDALRPEIIHWAAWYEHRLRLGNKPIFHLEAFVAKVMSLYKNYSIGLHDFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPU9</accession>
    <name>E3KPU9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">diphthine methyl ester synthase</fullName>
            <ecNumber evidence="4">2.1.1.314</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_12290</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + 4 S-adenosyl-L-methionine = diphthine methyl ester-[translation elongation factor 2] + 4 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:42652"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="Rhea" id="RHEA-COMP:10173"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="ChEBI" id="CHEBI:79005"/>
            <dbReference type="EC" id="2.1.1.314"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the diphthine synthase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">In the N-terminal section; belongs to the precorrin methyltransferase family.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.314" evidence="4"/>
    <dbReference type="EMBL" id="DS178299">
        <property type="protein sequence ID" value="EFP86334.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330753.2">
        <property type="nucleotide sequence ID" value="XM_003330705.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KPU9"/>
    <dbReference type="FunCoup" id="E3KPU9">
        <property type="interactions" value="638"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KPU9"/>
    <dbReference type="GeneID" id="10540347"/>
    <dbReference type="KEGG" id="pgr:PGTG_12290"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12290"/>
    <dbReference type="eggNOG" id="KOG3123">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_066040_1_0_1"/>
    <dbReference type="InParanoid" id="E3KPU9"/>
    <dbReference type="OrthoDB" id="2516at2759"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141133">
        <property type="term" value="F:diphthine methyl ester synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd11647">
        <property type="entry name" value="DHP5_DphB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.950.10:FF:000004">
        <property type="entry name" value="Diphthine synthase putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1010.10:FF:000004">
        <property type="entry name" value="Putative diphthine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01084">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004551">
        <property type="entry name" value="Dphthn_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00522">
        <property type="entry name" value="dph5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882:SF0">
        <property type="entry name" value="DIPHTHINE METHYL ESTER SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882">
        <property type="entry name" value="DIPHTHINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036432">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="12">
        <location>
            <begin position="1"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="113"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00035662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR036432-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP86334.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="312" mass="35178" checksum="7C466B7F9EA7B41F" modified="2012-04-18" version="2">MFYLIGIGMSSPEDITLSGLKTIQDSKKVYLEGYTSILIDSQLEDLQALYGKDIILADRDFIETRSDEILDQAAEEDVSLLVVGDPFGATTHADLLLRCTKKGIKYRVIHNVSILNAIGSIGINLYHFGQTVSIPFFNSNWRPKSWFKKINQNFNLGLHTLCLLDIKVKEQSDENLARGRKIYEKPRYMTITTAIEQIISIIEEFKQEDKDSEEEDSEERYTNRLTDPAQILCIAACRVTSKTEKFLVGSMAELAKLEPEEFGGPLHSLIILGDDPSKKNLNPVEIEFLSGFAVDKQSWFRLTGPSSNQLTS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQD6</accession>
    <name>E3KQD6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12467</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178300">
        <property type="protein sequence ID" value="EFP86511.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330930.2">
        <property type="nucleotide sequence ID" value="XM_003330882.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQD6"/>
    <dbReference type="GeneID" id="10540526"/>
    <dbReference type="KEGG" id="pgr:PGTG_12467"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12467"/>
    <dbReference type="HOGENOM" id="CLU_119660_0_0_1"/>
    <dbReference type="InParanoid" id="E3KQD6"/>
    <dbReference type="OrthoDB" id="10333445at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="161"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86511.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="197" mass="22115" checksum="D6758C835F8D107E" modified="2012-04-18" version="2">MNSSRDIFEETMAKYCPDWDYPDLHSILPEMDLDLKTGILTVYPSLPTVSGTRKDESLTMVQLPHLPKIDFFFFFCVRSVMLSIRVVLQNKKLMGSQAKSSSCQCPPRVQPPIFPIDTARALEEDACVCQKTQQNSVTKHEPDAPQLELLPVFDVQINLQPTEEPLANKHDPDASPSESPSIVKVEGDDNQGLKPEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQT6</accession>
    <name>E3KQT6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_13043</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the bacterial ribosomal protein bL17 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178301">
        <property type="protein sequence ID" value="EFP86661.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331080.2">
        <property type="nucleotide sequence ID" value="XM_003331032.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KQT6"/>
    <dbReference type="STRING" id="418459.E3KQT6"/>
    <dbReference type="GeneID" id="10540923"/>
    <dbReference type="KEGG" id="pgr:PGTG_13043"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13043"/>
    <dbReference type="HOGENOM" id="CLU_852787_0_0_1"/>
    <dbReference type="InParanoid" id="E3KQT6"/>
    <dbReference type="OrthoDB" id="275000at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1030.10:FF:000010">
        <property type="entry name" value="Mrpl8p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1030.10">
        <property type="entry name" value="Ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000456">
        <property type="entry name" value="Ribosomal_bL17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047859">
        <property type="entry name" value="Ribosomal_bL17_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036373">
        <property type="entry name" value="Ribosomal_bL17_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00059">
        <property type="entry name" value="L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14413:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN BL17M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14413">
        <property type="entry name" value="RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01196">
        <property type="entry name" value="Ribosomal_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64263">
        <property type="entry name" value="Prokaryotic ribosomal protein L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01167">
        <property type="entry name" value="RIBOSOMAL_L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="239"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="5">
        <location>
            <begin position="241"/>
            <end position="264"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP86661.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="339" mass="38846" checksum="675BE55D549F68F1" modified="2012-04-18" version="2">MRLAKLNRSMSHRRAMFRNLVSALIEHEQIKTTYPKAKAISRLAERVITWSKYGTENIFYQRKAESYLMNWVQVRKHLFHNLAKRYETRCGGYTRIHRVGYRANDHAPLAIIELVDNARDLKRDKVIRTISRELAQLELSQKPAYSNPSSPSSIKAKIPAAVWRNVPCKSEAQAKQAIEEFQRLLPEMTKKNMKKVLSEQYVQAYQYCQPPQIPTKEEALIEAAQAKRLRRDLLRKAAESIDSSSTASNDPAAPTTVSLINRTPTPKTKIGNFAHVKGIPRPLGPYTDFLYHIRLHFHRNLALLDPNKLAGRLNKGFAIPKPNLEESKILDQIPSFENA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3KS71</accession>
    <name>E3KS71_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13365</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="nucleotide sequence ID" value="XM_003331517.2"/>
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    <dbReference type="KEGG" id="pgr:PGTG_13365"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13365"/>
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    <dbReference type="InParanoid" id="E3KS71"/>
    <dbReference type="OrthoDB" id="10384913at2759"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="56"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="56"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87146.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="192" mass="21394" checksum="F53AADD8623A8069" modified="2012-04-18" version="2">MGKYAFLKRGANDSDESFHCCGKVEDLAREAYFFSNHAGSVETIKPATGLYSLTEETSTPVESTMQQSTSRSASTRPYYLPPPPMRPLYSKSTPVESEMHQSTSHSASTRPYYLPPPPIRPLYSKSVGNLNSVDQERKKAHRKSSFPDRILAEERFEDCSKAVTKQSPVWLGVILLNTDLPNTDLGKTDNAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>E3KTZ1</accession>
    <name>E3KTZ1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_13472</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178308">
        <property type="protein sequence ID" value="EFP87686.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332105.2">
        <property type="nucleotide sequence ID" value="XM_003332057.2"/>
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    <dbReference type="FunCoup" id="E3KTZ1">
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    <dbReference type="STRING" id="418459.E3KTZ1"/>
    <dbReference type="GeneID" id="10538919"/>
    <dbReference type="KEGG" id="pgr:PGTG_13472"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13472"/>
    <dbReference type="eggNOG" id="KOG0294">
        <property type="taxonomic scope" value="Eukaryota"/>
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    <dbReference type="HOGENOM" id="CLU_031466_0_0_1"/>
    <dbReference type="InParanoid" id="E3KTZ1"/>
    <dbReference type="OrthoDB" id="308449at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005730">
        <property type="term" value="C:nucleolus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004860">
        <property type="term" value="F:protein kinase inhibitor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="entry name" value="YVTN repeat-like/Quinoprotein amine dehydrogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051959">
        <property type="entry name" value="PAK1-Kinase_Regulator"/>
    </dbReference>
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        <property type="entry name" value="WD40/YVTN_repeat-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019775">
        <property type="entry name" value="WD40_repeat_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036322">
        <property type="entry name" value="WD40_repeat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001680">
        <property type="entry name" value="WD40_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44675:SF1">
        <property type="entry name" value="P21-ACTIVATED PROTEIN KINASE-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44675">
        <property type="entry name" value="PAK1 INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00400">
        <property type="entry name" value="WD40"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50978">
        <property type="entry name" value="WD40 repeat-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="WD_REPEATS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
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    <keyword evidence="1 3" id="KW-0853">WD repeat</keyword>
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        <location>
            <begin position="220"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="49"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="435"/>
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        </location>
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    </feature>
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        </location>
    </feature>
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    </feature>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP87686.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="495" mass="53704" checksum="0FA94B0AA9757062" modified="2012-04-18" version="2">MFGMEREMGMGSQVHGAEVRTLHHQLQASSSQWNIPKLSLEFSIMKRPALQSHPSGPSTQSGSKSKKPRNVPKPVQSQATQSHPKPDSITIVIGTYEHILYGLNLTFPPSPATPTFTTLFHFVAHRSPLNVLAVPSYPATPILASSAADSPLTLWSLSKRRCLGTLSCGILATDAGGAGREPGVKVARFDRSGKILVVGDETGGMTVYRTSDWALVRKFSGGGKGRVNDLAIEPQKGRIMLSVGQDRCLRMWDLSGGKDKGKPMASVRLGTESERLGWSPSGKKMVVVTGTIVTVYDTMMSPLFTFTSPRGRVHDAKFFIDQRSEEYLILGCDDSVGRIFHLGHSTVNSDTEPQCVAELIGHSNRVKSIELVTLPNEGDSTYAVTISSDGFCHVYQLLSEQWSDPENPYELEPVAKYDTGGCRLTCLSAVAVASGHDSQDEKNTPEEQDAQEDQSAQESGDDEEDEWSGIDLQANGEELSLDNSDSCPSSDSEPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVI7</accession>
    <name>E3KVI7_PUCGT</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14411</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
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            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178313">
        <property type="protein sequence ID" value="EFP88327.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332746.2">
        <property type="nucleotide sequence ID" value="XM_003332698.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KVI7"/>
    <dbReference type="GeneID" id="10535719"/>
    <dbReference type="KEGG" id="pgr:PGTG_14411"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14411"/>
    <dbReference type="HOGENOM" id="CLU_2265054_0_0_1"/>
    <dbReference type="InParanoid" id="E3KVI7"/>
    <dbReference type="OrthoDB" id="10273580at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="49"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88327.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="103" mass="11421" checksum="2E2D3DB3A3F8E97F" modified="2012-04-18" version="2">MQLHPSHPHQPPGASRITTKQDQINQDNSQSFLTWRCWKLDPASINKLVAADQPSSSSPAVDGQPANLDQQMNPADDPASRPAVDPLHELFRGGYWDKIPEIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>E3KVP7</accession>
    <name>E3KVP7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Mitochondrial import inner membrane translocase subunit TIM21</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Mitochondrial import inner membrane translocase subunit Tim21</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_14471</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TIM21 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178313">
        <property type="protein sequence ID" value="EFP88387.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332806.2">
        <property type="nucleotide sequence ID" value="XM_003332758.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KVP7"/>
    <dbReference type="STRING" id="418459.E3KVP7"/>
    <dbReference type="GeneID" id="10535860"/>
    <dbReference type="KEGG" id="pgr:PGTG_14471"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14471"/>
    <dbReference type="eggNOG" id="ENOG502S7ZJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_674619_0_0_1"/>
    <dbReference type="InParanoid" id="E3KVP7"/>
    <dbReference type="OrthoDB" id="436405at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005744">
        <property type="term" value="C:TIM23 mitochondrial import inner membrane translocase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030150">
        <property type="term" value="P:protein import into mitochondrial matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.320">
        <property type="entry name" value="Mitochondrial import inner membrane translocase subunit Tim21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013261">
        <property type="entry name" value="Tim21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038552">
        <property type="entry name" value="Tim21_IMS_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13032">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13032:SF6">
        <property type="entry name" value="MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08294">
        <property type="entry name" value="TIM21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0809">Transit peptide</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="141"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="267"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="375"/>
            <end position="397"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="10">
        <location>
            <begin position="271"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00020213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP88387.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="408" mass="46947" checksum="E55856F21DFEE176" modified="2012-04-18" version="2">MRSISNGFLNHLQPSTHSSLARIIQTSSQQLRQTSTLPDYRLQPKKWTRKILLIRSHDGQKIHSSSYVTSSAHRHNQSDKALIDELRRLSHGQESAQPMLGPFMMPRGTVNEKPLRHDEYHHWSRVGWSERIRRVFRMGRNLVIVSFGGCLGILVIYSITSELFAPSSTTNLMNMTIKTIQESEELNKILKSPIKFHTSPTPSGNSISKRTSGIPQSIQHQTGVVELRFWVESCKPSLLEGDWKSWEWWKSWIGPLITSEVYHYPSHRSADSNSNSSNDSQASGTENQSTRWWPGLLKSIMPNTLGRSSRASNDQKSWAERFFSNHGSFEHGEVVAELIKESNGEWRYKSLVIDFPDSQNVKYRLNVSNELKRKQRLANHRTAGQADGDEDNPKQPTRYRFWNRRINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>E3KW03</accession>
    <name>E3KW03_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_14056</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178314">
        <property type="protein sequence ID" value="EFP88478.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003332897.2">
        <property type="nucleotide sequence ID" value="XM_003332849.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KW03"/>
    <dbReference type="GeneID" id="10540781"/>
    <dbReference type="KEGG" id="pgr:PGTG_14056"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14056"/>
    <dbReference type="HOGENOM" id="CLU_894698_0_0_1"/>
    <dbReference type="InParanoid" id="E3KW03"/>
    <dbReference type="OrthoDB" id="10609800at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88478.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="311" mass="34437" checksum="2654FE0E1690DEDA" modified="2012-04-18" version="2">MSSFSKMTTQSLIPPTSREGFQRLLSRPSDALIPFLNSILAGRLTATITSIADTVQVIRSAPVMSTTATNQPERISQSLEQELRISELQSSHHHQQSSSQPSNPTEDPDREDKAEDSGTICYALVARTDKSEQIDVEIQVASRTLHQNEPEISGARMVSRHDSYMKMNRVYTITICDVPWPEMRFPIPSSLDNRWLFWLSPQWRGLGGTSGSAGRSFPVVDPAIQHCFVSLAIFRTVSADNVLDQLEKILWVFANGGPKRVPRALPDWVQADPTATLFVNLLLHNNDHQLGGGLEKGASDVPEIKHSSPVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3KWE9</accession>
    <name>E3KWE9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_14829</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178315">
        <property type="protein sequence ID" value="EFP88624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333043.1">
        <property type="nucleotide sequence ID" value="XM_003332995.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KWE9"/>
    <dbReference type="STRING" id="418459.E3KWE9"/>
    <dbReference type="GeneID" id="10541252"/>
    <dbReference type="KEGG" id="pgr:PGTG_14829"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14829"/>
    <dbReference type="eggNOG" id="KOG2877">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035066_5_0_1"/>
    <dbReference type="InParanoid" id="E3KWE9"/>
    <dbReference type="OMA" id="GMWMYST"/>
    <dbReference type="OrthoDB" id="196717at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014472">
        <property type="entry name" value="CHOPT"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414:SF77">
        <property type="entry name" value="CDP-ALCOHOL PHOSPHATIDYLTRANSFERASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10414">
        <property type="entry name" value="ETHANOLAMINEPHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015665">
        <property type="entry name" value="CHOPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="36"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="99"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="187"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="233"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="264"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="296"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="326"/>
            <end position="348"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP88624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="368" mass="41142" checksum="2AE4E0CF403D70E8" modified="2011-01-11" version="1">MPPWLAPNMITLIGLMFIVVNVITVIIYIPDLKADAPSWVYFSFAFGLFFYQTMDNVDGKQARKTGTSSPLGELFDHGIDSLNCVLGGLVQCAAVGTGHSLYAVFILIVACWPMYLSTWEEYHTGILYLGFVNGPTEGLLIAMAVLLCTGFGGVQLWSKPAENFIMLPQPILTYSEKYGHQLTLLDLFVSFVMFALVFGHAPSCFYNTWTAIRTNKSQPRSKQDLRILSFSQALLRTLPLLIFTFCSALWLASPHSHILKSEKIIEFGLMLCLIFGRIATRIILSHLTKSSFPFWSGMLIPIIGGSILINLPMIGLPVVLSPWRELVFLRLMFFFSIVGYVGSSLRTINRFCEVLRINCLTIRPKSPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KWP5</accession>
    <name>E3KWP5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_14678</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178316">
        <property type="protein sequence ID" value="EFP88712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333131.1">
        <property type="nucleotide sequence ID" value="XM_003333083.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KWP5"/>
    <dbReference type="STRING" id="418459.E3KWP5"/>
    <dbReference type="GeneID" id="10541502"/>
    <dbReference type="KEGG" id="pgr:PGTG_14678"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14678"/>
    <dbReference type="HOGENOM" id="CLU_1031106_0_0_1"/>
    <dbReference type="InParanoid" id="E3KWP5"/>
    <dbReference type="OMA" id="IHLMKEP"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP88712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="270" mass="31886" checksum="F51DB8B31662267B" modified="2011-01-11" version="1">MILELDELDLTLVHELDELDFNLMCELDELDLTLLDELDLTLLHELHELNELDELDFNLMRELDELDSTLLQKLDFNMILELDELDLTACELDELELNMMRELDELDFNLMRELDELDCSLELELDELDLTLLHELDELHFNLMRELDELDCSLELELDELDLTLLHELDELDLTLLRELDELDFNLMHELDELDLTLLRELDELDLTLLPQIYCWYTELGELELRWAHPALPCLRFQTSDPGTIIFGFKPKISVDKTRQNGPKSAPKSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>E3KWZ2</accession>
    <name>E3KWZ2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidase A1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_14224</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <text evidence="1 6">Belongs to the peptidase A1 family.</text>
    </comment>
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    <dbReference type="VEuPathDB" id="FungiDB:PGTG_14224"/>
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        <property type="taxonomic scope" value="Eukaryota"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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    </dbReference>
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        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
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    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Acid Proteases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001461">
        <property type="entry name" value="Aspartic_peptidase_A1"/>
    </dbReference>
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        <property type="entry name" value="Aspartic_peptidase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034163">
        <property type="entry name" value="Aspergillopepsin-like_cat_dom"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="Peptidase_aspartic_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966:SF1">
        <property type="entry name" value="ASPARTYL PROTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47966">
        <property type="entry name" value="BETA-SITE APP-CLEAVING ENZYME, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PEPSIN"/>
    </dbReference>
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    </dbReference>
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        <property type="entry name" value="ASP_PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="PEPTIDASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 6" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase A1" evidence="7">
        <location>
            <begin position="69"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
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        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR601461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000454"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="PROSITE" id="PS51767"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP88885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="390" mass="41971" checksum="F6CD039A3FEC08F1" modified="2011-01-11" version="1">MSQAILYPVKNTLRKESHRTGAAAYAKAVRRYNFKTGIFEGNKGRVLAIKEDGSDHEVPAESIAHDLEYVVPVTIGTPGVEIMLDFDTGSSDLWVWSSDLKASQPTASGHVVYDPKKSSTAKEVPGGTWKISYGDGSSASGVIYRDDIKIGDLHCSEQGIEVAQKLSSSFLNSQGSDGLLGLAWPQINTANPQQKTPMQNMIEKSITDQGLFTVCLKHDTDGKGFYSFGTICAEEAGVSEKDIAYAPIDNKQGFWAFESTKAKIGDEEIELSGNTAIADTGTTLALVSEAVTAALYKQIPGAVLDRSQGGYVYPVDAQVPDVQLAVGEKMYTIPGKSLAYGPPEKGMVFGGIQSRGNNPFDILGDTFLKSVYAVFDQKNVRIGFAQRNAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KX89</accession>
    <name>E3KX89_PUCGT</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP88962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333381.1">
        <property type="nucleotide sequence ID" value="XM_003333333.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KX89"/>
    <dbReference type="GeneID" id="10542403"/>
    <dbReference type="KEGG" id="pgr:PGTG_15165"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15165"/>
    <dbReference type="HOGENOM" id="CLU_148839_0_0_1"/>
    <dbReference type="InParanoid" id="E3KX89"/>
    <dbReference type="OrthoDB" id="2502638at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP88962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="155" mass="17693" checksum="DF2F11925A290684" modified="2011-01-11" version="1">MAYTYFNSSISWTETPVHVASLPSTLPYRSKTSFLKPTLVVEHRSTPIKVQFAAIRMPSVSILVLIIMALAWTSEARQLRPATIEANRRLPNRLWRRRVHVHNTESFVSRDSTQVDISAEVENSYEKESRRVQDLTESHDPSAFQPSFARAIPIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>E3KY20</accession>
    <name>E3KY20_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_15062</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
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            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178320">
        <property type="protein sequence ID" value="EFP89221.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333640.2">
        <property type="nucleotide sequence ID" value="XM_003333592.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KY20"/>
    <dbReference type="GeneID" id="10546858"/>
    <dbReference type="KEGG" id="pgr:PGTG_15062"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15062"/>
    <dbReference type="HOGENOM" id="CLU_082187_0_0_1"/>
    <dbReference type="InParanoid" id="E3KY20"/>
    <dbReference type="OrthoDB" id="2509126at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173842" evidence="2">
        <location>
            <begin position="23"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="20"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP89221.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="295" mass="33307" checksum="BE21216F234A9CDB" modified="2012-04-18" version="2">MTLLRYLLIAVLCLTHVHQTASEDTPEDDDDLSRTVVGGDPTDPSEVTTMRASGGRGGGGSRSSSKSTSPSTSSSSSGSTPYRTPYYYPYYYIPYGHGGYRSDGYGSPPPARILAWDYRLRLNKGNVTVVDTENGHPLYTISKAPHRGFIITNQRGDVLLRANDNKKASCGFGWKYHTPMNVSYSIDPRGPSTDRWKIELYGNGREEQPHTLKYYRAHQKNKGKIIDKTWGKTRNIQAARLDERDPLDKSKWEKDTLWKIGRVTTLEINDPTIGDHYFIGLWAMVHIRWDRCINA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KYD1</accession>
    <name>E3KYD1_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_15501</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178321">
        <property type="protein sequence ID" value="EFP89322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003333741.1">
        <property type="nucleotide sequence ID" value="XM_003333693.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KYD1"/>
    <dbReference type="GeneID" id="10547043"/>
    <dbReference type="KEGG" id="pgr:PGTG_15501"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_15501"/>
    <dbReference type="HOGENOM" id="CLU_855650_0_0_1"/>
    <dbReference type="InParanoid" id="E3KYD1"/>
    <dbReference type="OrthoDB" id="10294051at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="98"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="213"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="249"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="289"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP89322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="325" mass="36992" checksum="35550BAAC069BDE5" modified="2011-01-11" version="1">MKYASLTQLVKRHQPPSPLCGISPLQQVAPAPNQATTSSLDQSIVTPPLRNRRRRERLPFIDDEAKKSALDDWTAKLRSFPTANPGGSRHPRTYTLIGRGNLIPSPQRGTLSRPAAASQGKFTRRERRLPTPLAPTQAERPQPEGKRDRDYPYARVRAIREGMRGSRMSTSSQAKRPRQEEPSDRIDPYARVRAIRDRLPGSRMSTSSQAKRPRQEEPSDRIDPYARVRAMRDRLPGSRMSTSSQARRPRPEKPSDPIDDRIDPYARVRAIREGMRGSRMTTSSQARRPRPEKPSDPIDDRIDPYARVRAIRDGMRGSRKSTKRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>E3L1U2</accession>
    <name>E3L1U2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16543</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178334">
        <property type="protein sequence ID" value="EFP90517.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334936.2">
        <property type="nucleotide sequence ID" value="XM_003334888.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L1U2"/>
    <dbReference type="STRING" id="418459.E3L1U2"/>
    <dbReference type="GeneID" id="10530418"/>
    <dbReference type="KEGG" id="pgr:PGTG_16543"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16543"/>
    <dbReference type="HOGENOM" id="CLU_316445_0_0_1"/>
    <dbReference type="InParanoid" id="E3L1U2"/>
    <dbReference type="OrthoDB" id="2502962at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031267">
        <property type="term" value="F:small GTPase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030833">
        <property type="term" value="P:regulation of actin filament polymerization"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216:SF8">
        <property type="entry name" value="PAXILLIN, ISOFORM F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24216">
        <property type="entry name" value="PAXILLIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="238"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="719"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="813"/>
            <end position="842"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="31"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="90"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="129"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="193"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="1">
        <location>
            <begin position="813"/>
            <end position="825"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP90517.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="922" mass="102159" checksum="B9CFA6537EB6684E" modified="2012-04-18" version="2">MVKPKRTIKDHFKPITTTQQQNDNKNKPSATEPKTTATRRSTTRVTTSKTSPTSRPRTTTLKTSPKRTSISTLPKSSKTIKKNHTPSPHSSSSLSSLSSLDDNPTPTKRTTKTRPTTIQNNKNDHHIEIDDDDDDDDDDELPDHDFALPTKRKTIQTKPQKPDPKTNTPHQKSPSPSSSFNPARRRSSRLVPSTSKPSPSISIGSGSKTMIKLNKPAGPAKFSLEDILKDRRIRSEKQANLKRTREALGVSEDNEQTPDELLASHDPLLTPQKKSKTNSSHHDSKTVNGLDSICAQDPSTPPKAMIMLSDRTADLIISPSKAILLDEKDIDRFQSALDTQSTAHKPKIHKILRDDLVSGKLSGLNRGLNYRQLFHPNLLNPESTANPPPPSLPRIDYCGLEGVSKDPEVIEFVQELIDNLKYSIRHPDALPIDLTRVIPESLTNGTLADESGTMIMTHKTIFGLLFRPLINPEYPAPVARKAVVLIEALLATLSATPSLNRLHEFWLSTLQDGLCQLGLLPHLVFFGNYSNQDPARLESSNPSFAFTLTTATERFRLVLKLFTVLSSASYLNDRGRIWALTIALRVGVEPTSSSLRADVMEVISHVITTVYHGSSGEQSTKETKISLLVETLKVAIDLHTWTNQLDLVRLFPAHLPFRKALVIGVVNLAVGHLDAPAPVQEEGLYKMARWLAHITNRFTTDPPTREIFNTLKERSRRPYEPAPLASTVTEPKSQEDEPMGATQDSTMSSLIEIESTKKPIKEEKFRPAFAGYLGSEDAINEAQFSALVLLFQTLTMGLWDFLIDPKNLPISKPPAAPPSSNPTPPASFSSAPLPPADPSSSSFEAKLTGNVWITLVHQALESLDHSIKSCTSPHHQADRIKLKNIIFRLATALDFLNRESFLIAKGMDFKGQFKLDRFLKKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3L202</accession>
    <name>E3L202_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">GCM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_16603</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178334">
        <property type="protein sequence ID" value="EFP90577.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003334996.2">
        <property type="nucleotide sequence ID" value="XM_003334948.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L202"/>
    <dbReference type="GeneID" id="10530477"/>
    <dbReference type="KEGG" id="pgr:PGTG_16603"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16603"/>
    <dbReference type="HOGENOM" id="CLU_005992_0_1_1"/>
    <dbReference type="InParanoid" id="E3L202"/>
    <dbReference type="OrthoDB" id="2506598at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP90577.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1167" mass="131882" checksum="004071723E7291A9" modified="2012-04-18" version="2">MSSPSSNSLHNTLIEPSPPSVAIQHGLTTRHNWELPLAKDQVTFIDHDCRMDREGYPLYPNQNTTFVLQPGAQITNFGNVGYSKTTSNEKSKDNRWKVIRVRCLGVLLCNSEECDYAGPPPTGQGKIEELIGSNPTCPASGGTGWGLLRHTGFHNHPWPDPKKPDPLAKKMLALEIAKNPKAGALQLKVGDPNTSHEPYSSVIDIHESLGNSDRLRYYRCEVLERMDLVPNKSGGGDKFLHDMFLWHQRGLEVISSLFSSDSEHFTFQTAWMAARLLDHTQEENGSTYYKGGLISDVTYRFFDTGYLLTTSMYCQQINRWIPIQLSWIRGLSENYYKIHFATLFRQFMRPEILDEDRKDLARNIVDFSKAQSEGFISAYLEVFGGTDRVEARNKLKGCKQHYRASITRIKRNRAVIMADEVSVFERMCESLLVTSKEGSPTHEEKIDEIRHQFPKVKKWIDWWTMADVEAMLFPGRHGRSEDSPNELSGLPDTTNAQESMHRVYYMLSTGKKSLMIGLIELFAFVKALERDWEHVQRGMTIAYGSLPSKQVDVAQSIGWTKLTKRQKSAVNDGRPPDTSEQLLSDLEHAPKRGKLGRPKNSPNIIKNTPATYVSYVASIQPKSRNRCWLAAALESLYAVYSPLWLREPGGKTDDLFFFLVSHFTCWTTYEMTRSTQVRSILTQGSNKLFNEVHKRFPESFVYGDFASLDFFVELMLDPTRNKSKMLPKLFQVEENRKFTCSFHPGQQEHPRGSRTLSVLKITKSMFDANNIGYSNFVDLFNQWTSSGLLGVSGLQCRKCISKSRQDNQPSPNTPTVQSYLNEQSTLSFLGDTAPAHLYFHVEVAPILSRDTQREIMGGVNWPFKLTFKGEEYTLISRGFWTGAHYWGKVLRHFNGLTGVWLHDDHLNGGFAQLVSQVPGSISGALPDTSSLIYSRCWTPNETQFIDESIVKIKRDNPKVTISDIPFVNMQAVICSAFDSALPHLIGNPISPPEPVPYVKITLPDDDSQPDGHQMEIDAEDCKDVTLDPPSEKSLKIRIKRTKRTNQDVLDSVIPLNSDLLHVSGAPMASLTPSIPIEVPPKTIPATTQAKRMKTTRVRKDLPKVKVNQKKQFFLAPLTSADWNRLASASRAYWDEHWEAEGINDSTVKPFASAANVDLNSLHRPTIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3L392</accession>
    <name>E3L392_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17289</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178340">
        <property type="protein sequence ID" value="EFP91017.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335436.2">
        <property type="nucleotide sequence ID" value="XM_003335388.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L392"/>
    <dbReference type="GeneID" id="10528515"/>
    <dbReference type="KEGG" id="pgr:PGTG_17289"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17289"/>
    <dbReference type="HOGENOM" id="CLU_097645_0_0_1"/>
    <dbReference type="InParanoid" id="E3L392"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="21"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="38"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91017.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="171" mass="18728" checksum="A07B933CB990AE99" modified="2012-04-18" version="2">MVAKPVCKLDVGQLPPSFVAQSLEGSPQTPFPLDNKNKGNFQSPGDSPEGSFSYQAYGFGLGGKKMAGMLDKKIQCTTKSIKTHLLQGRGSDWPDDDKELHLPNPLRENNNTDDTEAIVKLRNEMTFYSKLFGAINNTFLPKSVAVAQAAVDYLKAEGSNHLQENLALEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3C2</accession>
    <name>E3L3C2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hydrophobin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17319</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178340">
        <property type="protein sequence ID" value="EFP91047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335466.1">
        <property type="nucleotide sequence ID" value="XM_003335418.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L3C2"/>
    <dbReference type="GeneID" id="10528545"/>
    <dbReference type="KEGG" id="pgr:PGTG_17319"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17319"/>
    <dbReference type="HOGENOM" id="CLU_1949890_0_0_1"/>
    <dbReference type="InParanoid" id="E3L3C2"/>
    <dbReference type="OrthoDB" id="2495628at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173970" description="Hydrophobin" evidence="1">
        <location>
            <begin position="21"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="129" mass="14064" checksum="A831523C292D6F9E" modified="2011-01-11" version="1">MQFSNLMIVFLSVALQLVAGKDAPYIFSCDKLTKDGKPVPQAICGIMIPDPKNPVPSQFSLVQAKMKLDSKGGYAKNNLCEDSGRVSLCCFENTATFDKSHTNTTRPLKLVEKQCVAPSPIAKRNTVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3M5</accession>
    <name>E3L3M5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16895</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178342">
        <property type="protein sequence ID" value="EFP91150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335569.1">
        <property type="nucleotide sequence ID" value="XM_003335521.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L3M5"/>
    <dbReference type="GeneID" id="10528812"/>
    <dbReference type="KEGG" id="pgr:PGTG_16895"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16895"/>
    <dbReference type="HOGENOM" id="CLU_1245905_0_0_1"/>
    <dbReference type="InParanoid" id="E3L3M5"/>
    <dbReference type="OrthoDB" id="2501445at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="71"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="10"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="99"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="126"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="225" mass="25393" checksum="1A1B1445C1BA7DAF" modified="2011-01-11" version="1">MHRLSIRYQRAHEDSDSDESFHCCGKPEDLLADPPTPPPEPKKHCAQSYREVLVPEDEEFYLYQSGLERQKQAERPISTCEPPPTSARASVPRILGRTHSAESDKTMVESEKPVVEEPLLVIRTGMTPSSPTRRMSQTPFDRISKVPSPKPKSRRASLNIFRASSVSRSSREKTPADVSTKSSKPPRARFSVLQTTRRFSKTLYDIKELSKRSTLQPQGSSKDIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3U6</accession>
    <name>E3L3U6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_16412</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178343">
        <property type="protein sequence ID" value="EFP91221.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335640.2">
        <property type="nucleotide sequence ID" value="XM_003335592.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L3U6"/>
    <dbReference type="GeneID" id="10528965"/>
    <dbReference type="KEGG" id="pgr:PGTG_16412"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16412"/>
    <dbReference type="HOGENOM" id="CLU_046891_1_1_1"/>
    <dbReference type="InParanoid" id="E3L3U6"/>
    <dbReference type="OrthoDB" id="2507040at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246">
        <property type="entry name" value="CCHC-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33246:SF51">
        <property type="entry name" value="MYB_SANT-LIKE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14223">
        <property type="entry name" value="Retrotran_gag_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="32"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="281"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="37"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="281"/>
            <end position="302"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91221.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="385" mass="42050" checksum="12FC520B54735FD2" modified="2012-04-18" version="2">MSTCETSLHTGFTNPNSKDYIIIPNQFDLDDSEESDVTLTRPQTTPKSPSHPNTQTIWGRTTKTPWIGLQLPRLELVLEIHFSATHVNYVLNDKPEVAKAKPSWSRDNKAICGTILRTIHPTNIRNVRHLKQDARRLWDALKRAHQDSSAGGVMFWLQKLTLSRMVDDDLQSHLDDMAKTFERLSSFITADSPLTPEDIYSASILTSLPSDWLSCAKGLRRKTRSEETTLVELSSAAKTKLATSKPNSDPGGPPARHCTFCNVPGHNLNQCRNVARLISAHKSSQTPSTPATAYSNRNQSNGRRPPQPPAKATPLVPFGTAKDGESDFSGSELKVTAGQAAVSLSSTLAAIPSGDANLDSGCLVSMTPDLLSVVLAKTNQTPVRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3X0</accession>
    <name>E3L3X0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_16436</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178343">
        <property type="protein sequence ID" value="EFP91245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335664.1">
        <property type="nucleotide sequence ID" value="XM_003335616.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L3X0"/>
    <dbReference type="GeneID" id="10528990"/>
    <dbReference type="KEGG" id="pgr:PGTG_16436"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_16436"/>
    <dbReference type="HOGENOM" id="CLU_1611599_0_0_1"/>
    <dbReference type="InParanoid" id="E3L3X0"/>
    <dbReference type="OrthoDB" id="2516446at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP91245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="165" mass="18356" checksum="D19DBE5523628E9F" modified="2011-01-11" version="1">MGCSSCDARKSGGLVESWRNPDQDGMTRCGEVGNRVKIVLNLKLEDDKVNVCYGYESMAQSEIVIKKMAQTSRGMDRSRRGDKIGKVALSNSRYCDGSTEETGEKNPRRATLVHGWCKALVLLLKPHNQAMGSSPTLTTIKSWLYRLWIGVFELSSVFSGLNFFP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>E3L3Z7</accession>
    <name>E3L3Z7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">PGTG_17129</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="13" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 10">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the oligosaccharyltransferase (OST) complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 10">Endoplasmic reticulum membrane</location>
            <topology evidence="2 10">Single-pass type I membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 10">Belongs to the OST1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178344">
        <property type="protein sequence ID" value="EFP91272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003335691.1">
        <property type="nucleotide sequence ID" value="XM_003335643.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L3Z7"/>
    <dbReference type="FunCoup" id="E3L3Z7">
        <property type="interactions" value="636"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L3Z7"/>
    <dbReference type="GeneID" id="10529018"/>
    <dbReference type="KEGG" id="pgr:PGTG_17129"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17129"/>
    <dbReference type="eggNOG" id="KOG2291">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031381_1_0_1"/>
    <dbReference type="InParanoid" id="E3L3Z7"/>
    <dbReference type="OMA" id="RYEYARE"/>
    <dbReference type="OrthoDB" id="310030at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008250">
        <property type="term" value="C:oligosaccharyltransferase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018279">
        <property type="term" value="P:protein N-linked glycosylation via asparagine"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007676">
        <property type="entry name" value="Ribophorin_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21049:SF0">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21049">
        <property type="entry name" value="RIBOPHORIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04597">
        <property type="entry name" value="Ribophorin_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0732">Signal</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="512"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="11">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="11">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="11">
        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="EFP91272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="539" mass="60186" checksum="89EEF81615B1E1EA" modified="2011-01-11" version="1">MPGPSTPPPPTPSDHRHEHQKEKMRWKQFSLATIGLSASSWLASVSSDQVVLDLPQFAVPSSLPARVTNYLRTIDISGTITREQLILHLRRTQHDSMNWYLPVEPHRAANLSSMEIYEGKANKTGGPGNRGLKIEPMGYDSGKEIHVIEVEWPHPDNDEAVITINSHFLGLTSPRPKYLAQDEGAKQGLYWEGDLLAGFGALKGTLTDDAQVKIKVKTPTPRVFQKHAPSGFDVQQTSGSNQVTFTSKINGAKLSDEPQVAHVHYFQPFPLMVIGNLTRLVEVSHWGNNVAIEDQIHLENRGPTLKGSFSRVVHHNAIYSRAPGATAHILTGLTLELPQGAMNPYYIDTIGNVSTSRFRPSMPSTATIGKNQPKFKKIKEAKSSKLELTPRYPIAGGWNYTFTVGYNLEAGRLLKIHEPGQYILKVPFFTNALDVPIDLANLRVRLPEGAFNIEVYTPFPVTELQDDLIDKTYLDTTGRPTISIKKLRCTEKHAEDIYITYFRPPIISLQKPLAIAGWMMALFVIAAGFRRFEWKIGHH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3L556</accession>
    <name>E3L556_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_17537</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178350">
        <property type="protein sequence ID" value="EFP91681.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336100.2">
        <property type="nucleotide sequence ID" value="XM_003336052.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L556"/>
    <dbReference type="GeneID" id="10531620"/>
    <dbReference type="KEGG" id="pgr:PGTG_17537"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17537"/>
    <dbReference type="HOGENOM" id="CLU_733904_0_0_1"/>
    <dbReference type="InParanoid" id="E3L556"/>
    <dbReference type="OrthoDB" id="2498123at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003174195" evidence="1">
        <location>
            <begin position="25"/>
            <end position="367"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91681.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="367" mass="41554" checksum="7EE7185AE57CF3B6" modified="2012-04-18" version="2">MKIASQLLFYLALHTLHAIQQALSNPVNHVPQKPTRSRRIESYFNLNTKSLDSLETSQSRSTVALEPGKLERLRPTMVKNGAEMNAMEAEHKRIEYLIEAVKKRDKILGDSLEEFYGHLKMPARARLYDLCTDSPLALCARHPAIQAPSPALTKPSSCEEAGTLSAPAHLIGRLFNCLGLDFLINQDETRISKIPRLSDSINSIPLQDEAPTRNSRTLGTLTCKNVNQEGVSKMKNEGEEIELHGEPLRIIQYMVSPLDDMWGRLESFMLCDLNLSAKDPLLAKWSANRHARLYNESRKLAISRGDIRRLNNMKKDWDMLYYVEAVFRLAEYISKYDLLPPNFISKIKILETNNLVPAIKAQISANT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>E3L5G9</accession>
    <name>E3L5G9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Oligopeptide transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_17794</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
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            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
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        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
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    <comment type="similarity">
        <text evidence="2">Belongs to the oligopeptide OPT transporter family.</text>
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        <property type="molecule type" value="Genomic_DNA"/>
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        <property type="nucleotide sequence ID" value="XM_003336165.2"/>
    </dbReference>
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    <dbReference type="KEGG" id="pgr:PGTG_17794"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17794"/>
    <dbReference type="HOGENOM" id="CLU_375130_0_0_1"/>
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        <property type="component" value="Unassembled WGS sequence"/>
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        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:oligopeptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004648">
        <property type="entry name" value="Oligpept_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004813">
        <property type="entry name" value="OPT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00728">
        <property type="entry name" value="OPT_sfam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22601">
        <property type="entry name" value="ISP4 LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03169">
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        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0571">Peptide transport</keyword>
    <keyword evidence="6" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="150"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="184"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="249"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="273"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="313"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="361"/>
            <end position="381"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="388"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="457"/>
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        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="511"/>
            <end position="530"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="542"/>
            <end position="562"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="650"/>
            <end position="672"/>
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    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="692"/>
            <end position="712"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008807"/>
        </source>
    </evidence>
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        <source>
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    </evidence>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="EMBL" id="EFP91794.2"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="740" mass="81197" checksum="F9353FB38C57C77E" modified="2012-04-18" version="2">MSCGRHPGPGTSQVQWHPWAAPISVKCDVLGTASRPRDISAAMASLGCTDFCEVPCVCSCQTRSKFTYDDELATQKRNQERRSVRLIDEIENAKDSTGLQGNSLKRPQRADSRGLAVKSPLHENENYSDPFDDMAVLDTEMRDDDGERTMTFRSVLIGVLTSILGAAIAEVFMFKPVHIHVDILFLQIICLLMGHFFATIPGPLWWNPGPLDTKETVFSVIMAASASAGVLGVEVIAAQDVMFEKRMSMIVSIMTLLSSQLIGYGIAGLLRPILVYPAEIIYPGVLPVVALFQSMSSQSQTTVKQMTFFKKAISAISLYEIIPTYLAPALSAVSIWCLTLPKVPAITNLFGGSLPAEGMGLMAFSGDWTLVGSHSPLFVPLLAQMTDWLAYFGCIVIYSGAYTANWFSGGHLPFISYNIFDKNGHRYNLTAAVMKNGTGNASVIEQLGPPSFSTSFVLSKSFLCMAASASVTIGMYTSLVSYLKDRKQIKMYGKISKPCPHREITKKMRDFPAYAYAVILVGATALAFLSSHLADSGLSPEALATSLLISFVLCLASGYFYGTLGIHLFVAREFVEFSPAVRAIEAKLNATFWLDYFDKPSCHSNARWPNISWQCNRYNVVHHVWLNQRKTMCAHAQRLEVGNLHASCSVVAQLIGTITGGLVHVLVMFSIINAQREVLLMPNGNGIFTGMYLSVIASQSTGWGLFSQAIYYPGKIYVMPHFSFSQVIFCHEEKLTQSPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3L5R9</accession>
    <name>E3L5R9_PUCGT</name>
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        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
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    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
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        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178354">
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        <property type="molecule type" value="Genomic_DNA"/>
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    <dbReference type="AlphaFoldDB" id="E3L5R9"/>
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    <dbReference type="KEGG" id="pgr:PGTG_17921"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_17921"/>
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    <dbReference type="InParanoid" id="E3L5R9"/>
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    <dbReference type="Proteomes" id="UP000008783">
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    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP91894.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="146" mass="16139" checksum="B733BD5BDF4CA091" modified="2012-04-18" version="2">MLSYQDDIPGHPLSPPVFLPEPTSTSTSAGPATMKASSGPPVLDNPVDQSNRHHRFHQDSMSKYRLAPTASAELPSTPQSTALRTEPNHENFLGSSWLESFLHHSAGNSNHHNHEVARQSSLQDVHQAQRIQSQSWISSQLFLDLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3L743</accession>
    <name>E3L743_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">hydroxyisourate hydrolase</fullName>
            <ecNumber evidence="5">3.5.2.17</ecNumber>
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    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_18353</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
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            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
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            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the hydrolysis of 5-hydroxyisourate (HIU) to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>5-hydroxyisourate + H2O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23736"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18072"/>
            <dbReference type="ChEBI" id="CHEBI:58639"/>
            <dbReference type="EC" id="3.5.2.17"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the transthyretin family. 5-hydroxyisourate hydrolase subfamily.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.17" evidence="5"/>
    <dbReference type="EMBL" id="DS178363">
        <property type="protein sequence ID" value="EFP92366.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336785.2">
        <property type="nucleotide sequence ID" value="XM_003336737.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L743"/>
    <dbReference type="STRING" id="418459.E3L743"/>
    <dbReference type="GeneID" id="10538030"/>
    <dbReference type="KEGG" id="pgr:PGTG_18353"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18353"/>
    <dbReference type="eggNOG" id="KOG2749">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG3006">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_553345_0_0_1"/>
    <dbReference type="InParanoid" id="E3L743"/>
    <dbReference type="OrthoDB" id="10265230at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033971">
        <property type="term" value="F:hydroxyisourate hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006144">
        <property type="term" value="P:purine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd05822">
        <property type="entry name" value="TLP_HIUase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.40.180:FF:000005">
        <property type="entry name" value="5-hydroxyisourate hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.180">
        <property type="entry name" value="Transthyretin/hydroxyisourate hydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014306">
        <property type="entry name" value="Hydroxyisourate_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023418">
        <property type="entry name" value="Thyroxine_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000895">
        <property type="entry name" value="Transthyretin/HIU_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023416">
        <property type="entry name" value="Transthyretin/HIU_hydrolase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036817">
        <property type="entry name" value="Transthyretin/HIU_hydrolase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02962">
        <property type="entry name" value="hdxy_isourate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10395:SF7">
        <property type="entry name" value="5-HYDROXYISOURATE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10395">
        <property type="entry name" value="URICASE AND TRANSTHYRETIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00576">
        <property type="entry name" value="Transthyretin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00189">
        <property type="entry name" value="TRNSTHYRETIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00095">
        <property type="entry name" value="TR_THY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49472">
        <property type="entry name" value="Transthyretin (synonym: prealbumin)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00768">
        <property type="entry name" value="TRANSTHYRETIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6" id="KW-0659">Purine metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transthyretin/hydroxyisourate hydrolase" evidence="10">
        <location>
            <begin position="380"/>
            <end position="492"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="1"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="63"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="9">
        <location>
            <begin position="154"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="176"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="194"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="9">
        <location>
            <begin position="222"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="387"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="490"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012609"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022631"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR600895-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00095"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP92366.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="493" mass="55173" checksum="858BFA60A8BA8FCD" modified="2012-04-18" version="2">MWNDTEQHHHHLPLENYPLDPQQPHHKKPKTTDSPTPSPSLPILTNSLELPPPTDSHHPNKDPSSLLQINPSQQQQQQHQHQHQHQQPTPQQQQKQQQQLQLQQNSNEPSSNEPVQPNPKPSTPELQPDLPVPSSSDNHFFTRTPKPSEENDKSISLAQAVAQFTQPNNDKPTNKSSPSSSTHSQSALHPLLKQQQQQHPQPQQQQPTQQPPPPHHHHHQQQQHQPQHTQTPFATSAPKIDQSTPNLACESPSAPNTSFEGVKFKTRSGGRPKDPVWVYFHTNGNGTDERATCKYCGWHVERPKAFRMRDHIAQCAQVDPARRAEMSQLIAFKEAAAAAKQEEKAHLATGSSVNAEILSSASKKRKMETPVHTKPALSFGKSPITSHVLDATSGKPGDDMGIRLDRLTANGFVLIANGTTDADGRCNTLLPANSRPEIGIYKVTFFSNEFYQKRGIVSFYPFVEITFEIKDPAEHYHIPLLLSPYAFSTYRGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>E3L7P2</accession>
    <name>E3L7P2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_18699</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178367">
        <property type="protein sequence ID" value="EFP92567.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003336986.2">
        <property type="nucleotide sequence ID" value="XM_003336938.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L7P2"/>
    <dbReference type="GeneID" id="10538726"/>
    <dbReference type="KEGG" id="pgr:PGTG_18699"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18699"/>
    <dbReference type="HOGENOM" id="CLU_1496947_0_0_1"/>
    <dbReference type="InParanoid" id="E3L7P2"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92567.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="180" mass="19864" checksum="0E417DA70E119D3B" modified="2012-04-18" version="2">MEPATAGTIPPRPLMAYPHRPRETLSAAESVHSHWPGETLSAAESVHSQRPREKKKKRKDIGPEIRCYPPSSSSEERSHTHLNFALPAVNHCPFAPIPAPHLAVTPLKTVQPALPIHNQPSLFQTSLTPIESINPLRRTVDATLDSAAEKDEKQHTTCMFVHGRELSSAESVQPSTTERN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8K5</accession>
    <name>E3L8K5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein mL54</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_18798</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Mitochondrion</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the mitochondrion-specific ribosomal protein mL54 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178375">
        <property type="protein sequence ID" value="EFP92880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337299.1">
        <property type="nucleotide sequence ID" value="XM_003337251.2"/>
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    <dbReference type="AlphaFoldDB" id="E3L8K5"/>
    <dbReference type="FunCoup" id="E3L8K5">
        <property type="interactions" value="19"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3L8K5"/>
    <dbReference type="GeneID" id="10542687"/>
    <dbReference type="KEGG" id="pgr:PGTG_18798"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_18798"/>
    <dbReference type="HOGENOM" id="CLU_144297_1_0_1"/>
    <dbReference type="InParanoid" id="E3L8K5"/>
    <dbReference type="OMA" id="FMKWRRK"/>
    <dbReference type="OrthoDB" id="10252718at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005762">
        <property type="term" value="C:mitochondrial large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013870">
        <property type="entry name" value="Ribosomal_mL54"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28595">
        <property type="entry name" value="39S RIBOSOMAL PROTEIN L54, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28595:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN ML54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08561">
        <property type="entry name" value="Ribosomal_L37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0496">Mitochondrion</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0809">Transit peptide</keyword>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="23"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="30"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035179"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP92880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="128" mass="14297" checksum="4A38E644D77F9CC7" modified="2011-01-11" version="1">MSSLIRTGLLRLSSHTESKQIYSRATSNLGDRHLASQPTQEKKTSQAKKTVPVSTIPAGSPIKGLAYIKGESDPIAKADDEYPSWLWTLLEPNMGVGHSDTPLRAIRRELNRENRNNIRKSNFLKAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8M7</accession>
    <name>E3L8M7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_19031</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178376">
        <property type="protein sequence ID" value="EFP92913.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337332.2">
        <property type="nucleotide sequence ID" value="XM_003337284.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L8M7"/>
    <dbReference type="STRING" id="418459.E3L8M7"/>
    <dbReference type="GeneID" id="10542722"/>
    <dbReference type="KEGG" id="pgr:PGTG_19031"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19031"/>
    <dbReference type="HOGENOM" id="CLU_1175936_0_0_1"/>
    <dbReference type="InParanoid" id="E3L8M7"/>
    <dbReference type="OrthoDB" id="10356781at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045290">
        <property type="term" value="F:D-arabinose 1-dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070485">
        <property type="term" value="P:dehydro-D-arabinono-1,4-lactone biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020471">
        <property type="entry name" value="AKR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42686">
        <property type="entry name" value="GH17980P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42686:SF1">
        <property type="entry name" value="GH17980P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP92913.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="236" mass="26356" checksum="91C97EA3E8CD4A31" modified="2012-04-18" version="2">MVIVADESFLAFNKRYTQFFEGVQDLFEVRVKNDYSSPQTTTPRTMISPTWPQQLFPLGTLLQMSHLIVTQLGCPLDLAMFYVCPTLQKSALSSYLPYFQAAGVVQIISASPLGNILLTTQGPPDWQPAPKALCQVIKEVAKSIQTTHHLSIEHISLLYRGLISARQPPRCTPICTPLADWRAGPHAKADWRAGSHAKDDWHGQMMATLNRFGDLLDHLAESFGSWLPIRRALGDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3L8N8</accession>
    <name>E3L8N8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">C2H2-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_19042</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178376">
        <property type="protein sequence ID" value="EFP92924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337343.1">
        <property type="nucleotide sequence ID" value="XM_003337295.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L8N8"/>
    <dbReference type="STRING" id="418459.E3L8N8"/>
    <dbReference type="GeneID" id="10542815"/>
    <dbReference type="KEGG" id="pgr:PGTG_19042"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19042"/>
    <dbReference type="HOGENOM" id="CLU_075838_1_0_1"/>
    <dbReference type="InParanoid" id="E3L8N8"/>
    <dbReference type="OMA" id="THHIEGP"/>
    <dbReference type="OrthoDB" id="6077919at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="C2H2-type" evidence="1">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP92924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="222" mass="26081" checksum="31086B6FFEBB2583" modified="2011-01-11" version="1">MAFCAPCNRSFNAYHSYLQHLNNSPQHRHDPNTLPYRPPAAGLHCVACGRVFYTRVEFVRHVYGCTRQIMDYECACGRQFARESSLVSHFERLTCPSWVDLRFVDNYFSGLCDPEGRFVHRERISQDPPMPNFIRDRRGSYWCRLCHLRFRTPHELMCHVWSPPHKNWGLRAYQCPSDRCQRMEFFCLSDLMAHLETGQCEEHLGRELSTMVIGLVDVVGYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3L9G3</accession>
    <name>E3L9G3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_19427</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178385">
        <property type="protein sequence ID" value="EFP93188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003337607.1">
        <property type="nucleotide sequence ID" value="XM_003337559.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3L9G3"/>
    <dbReference type="GeneID" id="10543338"/>
    <dbReference type="KEGG" id="pgr:PGTG_19427"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19427"/>
    <dbReference type="HOGENOM" id="CLU_129090_0_0_1"/>
    <dbReference type="InParanoid" id="E3L9G3"/>
    <dbReference type="OrthoDB" id="2507096at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP93188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="139" mass="15924" checksum="996F90783883573D" modified="2011-01-11" version="1">MDEDSFYRMRKIQRTPQSTFVNSQNVKAGLNVQHNCHNGGCELTETGDGFVERRKSKKKKLELTHTDHDQYIVNIASLSSAAWHRTFSEITFVSPGPLQWVNTLHDGLKKWGSIVEQKEKKVRKKSSTMARTTMDPSLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>E3LBK7</accession>
    <name>E3LBK7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_19749</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178419">
        <property type="protein sequence ID" value="EFP93932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338351.1">
        <property type="nucleotide sequence ID" value="XM_003338303.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3LBK7"/>
    <dbReference type="STRING" id="418459.E3LBK7"/>
    <dbReference type="GeneID" id="10535463"/>
    <dbReference type="KEGG" id="pgr:PGTG_19749"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_19749"/>
    <dbReference type="eggNOG" id="ENOG502S5U5">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_039761_2_2_1"/>
    <dbReference type="InParanoid" id="E3LBK7"/>
    <dbReference type="OMA" id="VRRIANM"/>
    <dbReference type="OrthoDB" id="5392716at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058913">
        <property type="entry name" value="Integrase_dom_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46177:SF1">
        <property type="entry name" value="INTEGRASE CATALYTIC DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24764">
        <property type="entry name" value="rva_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="2">
        <location>
            <begin position="157"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP93932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="474" mass="54635" checksum="042A1FAA49C7189F" modified="2011-01-11" version="1">MSARTLSRKRKEWGLRLCDLEQAPPPTPLSPKIRASLVSSHSKGMNLNEIQARLASETSVNVSIRTIKRYLKQLNLKLLRNDVTHGKVTIQQVYDAIHDARTRRLQSDAGYRRMRMILMREYDIQIPQRLVYDVLREIDPEGMAARLRGVCKRRIYRTNGPNHIWSSDGHDKLKRFGLTIYGFVDAWSRKILGMFVHVTNNDPRHIAVYFLQLASKAGGIPLLLTTDCGTETVKMARCMMELTYTHMEISLEDAAKRMHYTKSTHNQKIESLWSQMMKQHNRAIKHDIQSHIEDGSYDDQDDVQKLLFLFLWVPVLQASVDSWVDTYNNYRKRRDHLTTLPTGVSSEFAYSTPEYFGTTNQLLKMPSSDIDLMLQTDYPNLDLLFAHTPLDFHEVAIEVMGNLGWDFSQINTGNVWFVFHEMLPHVVAQYIPPTDSSSEYESHLSNESGSHISNVSESYISTTCNHSSFEYETP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>E3NXF2</accession>
    <name>E3NXF2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">3,4-dihydroxy-2-butanone 4-phosphate synthase</fullName>
            <shortName evidence="12">DHBP synthase</shortName>
            <ecNumber evidence="3 12">4.1.99.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_20167</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>D-ribulose 5-phosphate = (2S)-2-hydroxy-3-oxobutyl phosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18457"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="4.1.99.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="12">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="12">Binds 2 divalent metal cations per subunit. Magnesium or manganese.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 12">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11 12">Belongs to the DHBP synthase family.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.12" evidence="3 12"/>
    <dbReference type="EMBL" id="DS989934">
        <property type="protein sequence ID" value="EFP94251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338670.1">
        <property type="nucleotide sequence ID" value="XM_003338622.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NXF2"/>
    <dbReference type="FunCoup" id="E3NXF2">
        <property type="interactions" value="107"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3NXF2"/>
    <dbReference type="GeneID" id="10527342"/>
    <dbReference type="KEGG" id="pgr:PGTG_20167"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20167"/>
    <dbReference type="HOGENOM" id="CLU_020273_3_0_1"/>
    <dbReference type="InParanoid" id="E3NXF2"/>
    <dbReference type="OMA" id="YNTHAVE"/>
    <dbReference type="OrthoDB" id="60371at2759"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00399"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005758">
        <property type="term" value="C:mitochondrial intermembrane space"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008686">
        <property type="term" value="F:3,4-dihydroxy-2-butanone-4-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.870.10:FF:000002">
        <property type="entry name" value="3,4-dihydroxy-2-butanone 4-phosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.10">
        <property type="entry name" value="DHBP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00180">
        <property type="entry name" value="RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000422">
        <property type="entry name" value="DHBP_synthase_RibB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00506">
        <property type="entry name" value="ribB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327:SF18">
        <property type="entry name" value="3,4-DIHYDROXY-2-BUTANONE 4-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327">
        <property type="entry name" value="GTP CYCLOHYDROLASE II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00926">
        <property type="entry name" value="DHBP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="YrdC/RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0318">Glutathionylation</keyword>
    <keyword evidence="10 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 12" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0686">Riboflavin biosynthesis</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00060730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP94251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="220" mass="24236" checksum="919ADD8EA3A28ECA" modified="2011-01-11" version="1">MASSPADQASSSCKLSTIEEALESFRNGECLVVVDDLERENEGDLVVAAAKITTEMMAWIIKHSSGYVCIALPGERLDQLEIPLMIETNTEKHKTAYTHTVDYKHNIATGISAHDRALTASKLSDPSSRPEDFTRPGHMVPLRAREGGVLTRRGHTEAAIDLCRLAGLPAAGLICELVKPDCPLGSMARRDDCAQFASQWNLKMISIEDLVQYRRRQLES</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>E3NXY7</accession>
    <name>E3NXY7_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">PB1 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_20392</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS989997">
        <property type="protein sequence ID" value="EFP94436.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003338855.2">
        <property type="nucleotide sequence ID" value="XM_003338807.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3NXY7"/>
    <dbReference type="GeneID" id="10527728"/>
    <dbReference type="KEGG" id="pgr:PGTG_20392"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20392"/>
    <dbReference type="HOGENOM" id="CLU_973660_0_0_1"/>
    <dbReference type="InParanoid" id="E3NXY7"/>
    <dbReference type="OrthoDB" id="661148at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05992">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.90">
        <property type="entry name" value="Phosphatidylinositol 3-kinase Catalytic Subunit, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053793">
        <property type="entry name" value="PB1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000270">
        <property type="entry name" value="PB1_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00564">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00666">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54277">
        <property type="entry name" value="CAD &amp; PB1 domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51745">
        <property type="entry name" value="PB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PB1" evidence="2">
        <location>
            <begin position="48"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="145"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="145"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="227"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP94436.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="286" mass="30588" checksum="471B995B44ADCDFE" modified="2012-04-18" version="2" fragment="single">MVCPTLTVAPQRPLPVFSQPQYLQDIRIVDFEQHKTFIQTTMSSQQAPLFIKIKHANSTRKVRVPNQPAPVWSKLSAAIVDRFGIPEGQPIGLQYLDPEGDIITISSQVEFDELWHEITLAAKPNQKGGVDQRTLSLELILIDHQPTQPKPSSDLTKVHHPEAGSISTEGSTFQLIPTPADGSSTSTVDPASDSAHHGPAAAEGNNPIKGASDPTPGTAASDPPEYPINTSPSPNSRSPPVDSNLGLSALITAIDAIAPKLQDTLGGYAQMIVAGERPAQRYLSGH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>H6QPQ9</accession>
    <name>H6QPQ9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_20807</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EHS64106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890515.1">
        <property type="nucleotide sequence ID" value="XM_003890466.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QPQ9"/>
    <dbReference type="GeneID" id="13542975"/>
    <dbReference type="KEGG" id="pgr:PGTG_20807"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_20807"/>
    <dbReference type="HOGENOM" id="CLU_160862_0_0_1"/>
    <dbReference type="InParanoid" id="H6QPQ9"/>
    <dbReference type="OrthoDB" id="10298878at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS64106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="92" mass="10038" checksum="A638F4F351A3C330" modified="2012-04-18" version="1">MAAGGNLGWVVDRHTIRVRLSIICGVLSGQLQAVGILHHVEGLSDSQFILQVEFQYSVTTLATCLVLLGMSEGIPFARAELRVLRFDQVVES</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQF0</accession>
    <name>H6QQF0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21108</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EHS62561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890274.1">
        <property type="nucleotide sequence ID" value="XM_003890225.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQF0"/>
    <dbReference type="GeneID" id="13541172"/>
    <dbReference type="KEGG" id="pgr:PGTG_21108"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21108"/>
    <dbReference type="HOGENOM" id="CLU_2795155_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQF0"/>
    <dbReference type="OrthoDB" id="2509956at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS62561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="68" mass="8091" checksum="0286C57B068621D9" modified="2012-04-18" version="1">MLPPNLDEDDSLQGIALWHFLFVQRCQERNMVYIRAGREVFKLPDRWEPWKDERIPAASKTHLDLANL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQH6</accession>
    <name>H6QQH6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_21184</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="2" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EHS62676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890222.1">
        <property type="nucleotide sequence ID" value="XM_003890173.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQH6"/>
    <dbReference type="GeneID" id="13541289"/>
    <dbReference type="KEGG" id="pgr:PGTG_21184"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21184"/>
    <dbReference type="HOGENOM" id="CLU_024139_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQH6"/>
    <dbReference type="OrthoDB" id="10274719at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EHS62676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="436" mass="49702" checksum="7FE22E5984CBBB0D" modified="2012-04-18" version="1">MRQEMQDQLNWVLTGFFVNINHHTLELSQADIASLQNHGSDISNAYHVIVTPRAEKPGLLAGIGSGTGDLQKSNAKLVLHILGKIEESCIILEDPKRFTTAKVNVNALKLMYRLNDFSVGLLAALEKHSLISDEFIRGILNDRYRGKLIFNWTSGVHVFNPAHFQVLYMSIDFRLSMKSSPSTHLIHGLLKHLDSVTWKNLERSCLDAQLTGISLDPELEEHMASFKQLIQLRNEFMALTSPAVERLTDHAFQGHIHQVLQDLIRRIFSTVDGEALDHYLIRIKLSYNVLRFLIEYCVKPSSLSEEYMRKLRKLADFPKLQALETYMIEYSDILQKAYVELCKTLQRSPDRDLTEKYGLELLDGRYLVDMDKAEQPLLISLGKNQDTNMQTPKLESLEIINELKKEIELVSNTLGDPNQHGVESQAKDLAFALGIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQI6</accession>
    <name>H6QQI6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21193</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178272">
        <property type="protein sequence ID" value="EHS62686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890232.1">
        <property type="nucleotide sequence ID" value="XM_003890183.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQI6"/>
    <dbReference type="GeneID" id="13541231"/>
    <dbReference type="KEGG" id="pgr:PGTG_21193"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21193"/>
    <dbReference type="HOGENOM" id="CLU_143009_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQI6"/>
    <dbReference type="OrthoDB" id="96345at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324">
        <property type="entry name" value="EXPRESSED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33324:SF2">
        <property type="entry name" value="MYB_SANT-LIKE DNA-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="136"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="15"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="144"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="168"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="178" mass="20356" checksum="F8804491D2EEEBEA" modified="2012-04-18" version="1">MSSSPHSQRVTRRQAGRDHRWDRDAINGGPSSLTILLNWLPTPGNYSRWDAPGNPDREGICMEILELMENEGIHHRHVWGINHRIQLLRRSYNTARDFIAHAQHEPNIHDGIILLYARRVCPHWDLLHPIMGPQTADTHAEQAENIDNEDATSEVSEDESTNTSRARIGRENKPSVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQM6</accession>
    <name>H6QQM6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">F-box domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21210</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EHS62744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890161.1">
        <property type="nucleotide sequence ID" value="XM_003890112.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQM6"/>
    <dbReference type="GeneID" id="13541336"/>
    <dbReference type="KEGG" id="pgr:PGTG_21210"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21210"/>
    <dbReference type="HOGENOM" id="CLU_029800_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQM6"/>
    <dbReference type="OrthoDB" id="2498672at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.10.10">
        <property type="entry name" value="Ribonuclease Inhibitor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001810">
        <property type="entry name" value="F-box_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032675">
        <property type="entry name" value="LRR_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12937">
        <property type="entry name" value="F-box-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52047">
        <property type="entry name" value="RNI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50181">
        <property type="entry name" value="FBOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="F-box" evidence="1">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="408" mass="46930" checksum="94A68886F6DFC873" modified="2012-04-18" version="1">MANILDLPREIFERIFMHLITTDWEWGHELNRADCIAYVACHLRQVCRRWADWLYESHLYRTLTFSSASTAIAFANHIRERSKLLPRAKCQYLEINHFWPWKHLPGHAYNYNEITSEALEVLIDLFSDTIVTLDLKFIEFFTLPSETIEAIGRIKNLRNLTFEPSRYPSGMDEFPPSDPDDFNTLLMVTRGLKSLRLGLPVSLRPESGLPPITYLDLNFQFEHIDCIISLSIALKPHLKALSLRNYSTIFDAQPLLPMYENLRETLEGLSVSESCFLEPILDFNFPKLQVVSVHDWRGSIANWLSQDLFLYAPIQALAISSRSVKGLQQAFPAHPLANLPKLQQLVIMNTRSSFSPTPSYLSVSKSHRVECTYLDHGEMSLIMKLLMPTDLLQVKKPRHSPLSFIPAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>H6QQX4</accession>
    <name>H6QQX4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Extracellular membrane protein CFEM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21286</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EHS62937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890097.1">
        <property type="nucleotide sequence ID" value="XM_003890048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QQX4"/>
    <dbReference type="GeneID" id="13541556"/>
    <dbReference type="KEGG" id="pgr:PGTG_21286"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21286"/>
    <dbReference type="eggNOG" id="ENOG502SKVV">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_2387244_0_0_1"/>
    <dbReference type="InParanoid" id="H6QQX4"/>
    <dbReference type="OrthoDB" id="2507685at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003605574" description="Extracellular membrane protein CFEM domain-containing protein" evidence="1">
        <location>
            <begin position="24"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="94" mass="9295" checksum="E782E6D6671B9F6E" modified="2012-04-18" version="1">MFSGNSALLVVVLSSLSLAQAAGDNPPNPAGPTIPACAANCMSIKIKEAAAWFGQSAVSSYCTASSQAGDASAISSSSALLFTAISSCAFSLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>H6QR55</accession>
    <name>H6QR55_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Defect at low temperature protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">DLT1</name>
        <name evidence="9" type="ORF">PGTG_21322</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Required for growth under high-pressure and low-temperature conditions.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the DLT1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EHS63030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890034.1">
        <property type="nucleotide sequence ID" value="XM_003889985.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QR55"/>
    <dbReference type="GeneID" id="13540934"/>
    <dbReference type="KEGG" id="pgr:PGTG_21322"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21322"/>
    <dbReference type="eggNOG" id="ENOG502RAJJ">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_702351_0_0_1"/>
    <dbReference type="InParanoid" id="H6QR55"/>
    <dbReference type="OMA" id="SIACPRW"/>
    <dbReference type="OrthoDB" id="337038at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038869">
        <property type="entry name" value="DLT1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40021">
        <property type="entry name" value="DEFECT AT LOW TEMPERATURE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40021:SF1">
        <property type="entry name" value="DEFECT AT LOW TEMPERATURE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="67"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="357"/>
            <end position="377"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="8">
        <location>
            <begin position="7"/>
            <end position="40"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU367100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS63030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="457" mass="49757" checksum="0D8635A11AE182A5" modified="2012-04-18" version="1">MGTPNKPSNQSISSSASSSSPSHPFPRSSSSSSSSSSSSPQLDNPTNSLSSSSSSSSSSSFLHTSSFIFFVLLSLAIACLCVYDIIRQTTSGLRSSTIQLTSIALGSCLLVLVVSFFTSISRYLTVKHTLTLIPKPYIPISQVDLPNPIFQTINQEYGRIAIITYLASTPDNPHQPPPQERVGLAGWGKPGTIHHGKRFADQILKDFKTLEHALDGLLPIQPIPSIPTRQSSTIDGPLTRYLHILNPSSPTQPTPILLTPAQKHILNRYALLIQKSLAPSSIVTESDYQVACKCLVILVDQIQLSRSPDWPNPDGYGPKGHPLYQGIRFKDAIIETAIKLEQSLAQKLLVQSVKPSKTASLTPPSTMQTSRPGSGMRTTTITGTMNGKEAIENLVKKLFPLIYPPFLPSAFGPIITGYENLIKEIAFLHPQLERSLEEYEYALRCIGSLISHVQKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>H6QRX3</accession>
    <name>H6QRX3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Tc1-like transposase DDE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21588</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EHS63457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889742.1">
        <property type="nucleotide sequence ID" value="XM_003889693.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QRX3"/>
    <dbReference type="GeneID" id="13543122"/>
    <dbReference type="KEGG" id="pgr:PGTG_21588"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21588"/>
    <dbReference type="HOGENOM" id="CLU_056788_1_0_1"/>
    <dbReference type="InParanoid" id="H6QRX3"/>
    <dbReference type="OrthoDB" id="2497579at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038717">
        <property type="entry name" value="Tc1-like_DDE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047655">
        <property type="entry name" value="Transpos_IS630-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033545">
        <property type="entry name" value="transpos_IS630"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46564:SF1">
        <property type="entry name" value="TRANSPOSASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13358">
        <property type="entry name" value="DDE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tc1-like transposase DDE" evidence="1">
        <location>
            <begin position="135"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS63457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="315" mass="35901" checksum="E693012DC737ECCC" modified="2012-04-18" version="1">MSLRGETRQEINETLDLKISADSIRRWNQLYERTRSVIRNPAFYQRRGRPIRVSRDESQFIMDALDLEPTLYLDEIRAHIDAMTGERHPIATIHNEIKYRLRLTCKKARTVHPAQCALRRAAFVSQIGFIPSNHLVFLDETGVSIKTHSRDHAYALKGRRTVRLPRPITASRISVLPAVSLDGLLAVIAQEGTMYRLDLEYFLEEFLSNSFIELPNMNPFPDRNSVLVLDNASLHHGGGVARLCEDRGVLLVYLPPYSPDMNPIEKVFSVLKSQLKRRQILTGTVKDIDIIKDVLPEIVTPELMASLFRGSNYAA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>H6QT74</accession>
    <name>H6QT74_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21993</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="3" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178308">
        <property type="protein sequence ID" value="EHS64028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889277.1">
        <property type="nucleotide sequence ID" value="XM_003889228.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QT74"/>
    <dbReference type="GeneID" id="13542027"/>
    <dbReference type="KEGG" id="pgr:PGTG_21993"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21993"/>
    <dbReference type="HOGENOM" id="CLU_2360779_0_0_1"/>
    <dbReference type="InParanoid" id="H6QT74"/>
    <dbReference type="OrthoDB" id="10272714at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="14"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="72"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="85"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS64028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="96" mass="10812" checksum="242C6B8705597107" modified="2012-04-18" version="1">MSNPTPFFTLDLAKRTSDEPRGQPACHHTGRRSSRPRNLYLWTALGYKECQGLLELDKWGGQDLDKQGLVDLQKRPSSGQGFDLAEVRPVSREDAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>H6QTX0</accession>
    <name>H6QTX0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">cellulase</fullName>
            <ecNumber evidence="3">3.2.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">PGTG_22241</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="14" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.</text>
            <dbReference type="EC" id="3.2.1.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the glycosyl hydrolase 5 (cellulase A) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.4" evidence="3"/>
    <dbReference type="EMBL" id="DS178323">
        <property type="protein sequence ID" value="EHS64384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003889056.1">
        <property type="nucleotide sequence ID" value="XM_003889007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QTX0"/>
    <dbReference type="STRING" id="418459.H6QTX0"/>
    <dbReference type="GeneID" id="13541821"/>
    <dbReference type="KEGG" id="pgr:PGTG_22241"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22241"/>
    <dbReference type="eggNOG" id="ENOG502QXN4">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_029718_1_1_1"/>
    <dbReference type="InParanoid" id="H6QTX0"/>
    <dbReference type="OrthoDB" id="5823761at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008810">
        <property type="term" value="F:cellulase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030245">
        <property type="term" value="P:cellulose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009251">
        <property type="term" value="P:glucan catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.80:FF:000124">
        <property type="entry name" value="Exported cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001547">
        <property type="entry name" value="Glyco_hydro_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018087">
        <property type="entry name" value="Glyco_hydro_5_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34142">
        <property type="entry name" value="ENDO-BETA-1,4-GLUCANASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34142:SF1">
        <property type="entry name" value="GLYCOSIDE HYDROLASE FAMILY 5 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00150">
        <property type="entry name" value="Cellulase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00659">
        <property type="entry name" value="GLYCOSYL_HYDROL_F5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="6" id="KW-0136">Cellulose degradation</keyword>
    <keyword evidence="8 10" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003605628" description="cellulase" evidence="11">
        <location>
            <begin position="25"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="Glycoside hydrolase family 5" evidence="12">
        <location>
            <begin position="67"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EHS64384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="358" mass="39708" checksum="6851C1F46389CA02" modified="2012-04-18" version="1">MLLSSYKLFSLLPMTIAFGQGVKALNLFRGPSQQDVKRWAGVNLAGLEFGMQNTGTMNQDPMEPPPISQIQHFRAENVTAFRIPISWQRMQPKIMGPLDPDSLQLLSKYVNSALACKASVIIDLHNYARRDGKVIGEAEDLPASSLVDFWTRIAQKFGEFPEVGFGIMNEPHDVKLDIWIETLQAVVTAIRKAGATNKIILPADNWSHLQTFATSYNAGMSKIHNPDGSCTGLIFEIHQYFDSDGSGTSAGCPVPHTKELQEVVALLAKDDRQAMITEFGGGHNPDCPSVLADFVEEAHKSFPQILGYFLWGAGAFHQDYTLVVTVEKDGEWVDQSNFLAIQKFMDPGPKRNQIGWMH</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>H6QVI4</accession>
    <name>H6QVI4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_21097</name>
        <name evidence="3" type="ORF">PGTG_22751</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="4" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="submission" date="2012-02" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EHS62549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="EMBL" id="DS178432">
        <property type="protein sequence ID" value="EHS63037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003888480.1">
        <property type="nucleotide sequence ID" value="XM_003888431.1"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890262.1">
        <property type="nucleotide sequence ID" value="XM_003890213.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QVI4"/>
    <dbReference type="GeneID" id="13540980"/>
    <dbReference type="GeneID" id="13542230"/>
    <dbReference type="KEGG" id="pgr:PGTG_21097"/>
    <dbReference type="KEGG" id="pgr:PGTG_22751"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_21097"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22751"/>
    <dbReference type="HOGENOM" id="CLU_130547_0_0_1"/>
    <dbReference type="InParanoid" id="H6QVI4"/>
    <dbReference type="OrthoDB" id="2510374at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="67"/>
            <end position="88"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EHS62549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EHS63037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="88" mass="9687" checksum="EDAADDDF2D306B3D" modified="2012-04-18" version="1">MPLIDRITPTDLQTTRIRIAQLVEGTSLADVMTTFVCAVEEMDGATADGLCLNVKLVRVIRPPVHIPTKQEAQDGSPLLHRISKRPRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2012-04-18" modified="2025-10-08" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>H6QVP8</accession>
    <name>H6QVP8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_22819</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference evidence="10" key="1">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS990051">
        <property type="protein sequence ID" value="EHS63696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003890851.1">
        <property type="nucleotide sequence ID" value="XM_003890802.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="H6QVP8"/>
    <dbReference type="GeneID" id="13541133"/>
    <dbReference type="KEGG" id="pgr:PGTG_22819"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_22819"/>
    <dbReference type="HOGENOM" id="CLU_003181_2_1_1"/>
    <dbReference type="InParanoid" id="H6QVP8"/>
    <dbReference type="OrthoDB" id="431497at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005769">
        <property type="term" value="C:early endosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005794">
        <property type="term" value="C:Golgi apparatus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005247">
        <property type="term" value="F:voltage-gated chloride channel activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45711">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45711:SF6">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0868">Chloride</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="23"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="52"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="126"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="166"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="257"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="301"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="327"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EHS63696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="571" mass="62153" checksum="A6CDE030154BD706" modified="2012-04-18" version="1">MLTKAVGLALSVGSGLTLGKEGPLVHIACCIGNIFTRLFPKFDRNEGKRREMLSAACAAGVAVAFGAPIGGVLFSLEEVSYFFPPRVMWRSCWCAIVGAATLRVLDPFKTGKTVLFEVTYDQQWHFIELSGFILLGLVSGVLGAWLSKLNVWWTKTFRKLPCIDRHPVLEVLLVAFVTCLLAFSNRFMKLAGTELVYEMLAECPIIDPSDPTGSSISGACISDPKDTAQLILNIGIAVVLKFLITVVTFGIKCPAGLFVPSLCIGAMMGRILGYLVEYAYHSHPELSVFQICDPSRPFGQACIIPGVWAMVGAAAMLAGVTRTTLSLAVIMVELTGSLVYILPISMSVLVAKTLADTIEHRSIYDLCMNLSELPYLDAKSEYLHYAKPEDIMDRNAEVIILNGELRASDLRQSIKNMLEAPQLGSGFPLLETSENGDTRISGYVGLVELEHCLSTIEGDPICTFDGADPDVAPNGGLSPNYELPVDFGYLVDHAPVTVSVQTPMELIHEIFVRLGVRYLVVQNHNGLYLGIIEKNRHHTTIPTPNGKEDAHFEALIMGSNSCLSLSSLLFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="67" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQB5</accession>
    <name>E3JQB5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="6">CAMK protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_00435</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307485.1">
        <property type="nucleotide sequence ID" value="XM_003307437.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQB5"/>
    <dbReference type="STRING" id="418459.E3JQB5"/>
    <dbReference type="GeneID" id="10527808"/>
    <dbReference type="KEGG" id="pgr:PGTG_00435"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00435"/>
    <dbReference type="eggNOG" id="KOG1152">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_459360_0_0_1"/>
    <dbReference type="InParanoid" id="E3JQB5"/>
    <dbReference type="OMA" id="AVPRANM"/>
    <dbReference type="OrthoDB" id="10252171at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035556">
        <property type="term" value="P:intracellular signal transduction"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045719">
        <property type="term" value="P:negative regulation of glycogen biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000314">
        <property type="entry name" value="Serine/threonine protein kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017441">
        <property type="entry name" value="Protein_kinase_ATP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008271">
        <property type="entry name" value="Ser/Thr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346:SF72">
        <property type="entry name" value="CAMK PROTEIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24346">
        <property type="entry name" value="MAP/MICROTUBULE AFFINITY-REGULATING KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00220">
        <property type="entry name" value="S_TKc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00107">
        <property type="entry name" value="PROTEIN_KINASE_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00108">
        <property type="entry name" value="PROTEIN_KINASE_ST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0418">Kinase</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="5">
        <location>
            <begin position="232"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="1"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="81"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="4">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="4">
        <location>
            <begin position="100"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="261"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP74479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="593" mass="66063" checksum="F6B8FB71991C2C0E" modified="2011-01-11" version="1">MKSAYQLSPIPPFRAQTPKTTSSSLSTSSNSQATAFTESSDGADASSQLTSFSFNKKRPPPLKGYSRSNLSLIDYDGPSAPLPSSPLGTEQIINDNNGASPRSGSRPLSPLSKGQQDSHRAPVKSRFLPGRPSNYVPVVPRANMSASKHRNSNHGSIFMPNTDANLTPPATPNSREGVFLQAKSLLSKSINPQLPPTPSTSPYQKAAPLVPTTVSAKALSTHKLHPVFLANYTLGDELGSGGFGFVVQATRNEDLMQCAVKFIYKEKIPQQAWVKDEHWEDDSDTNGIMRTKDGIKRVPLEAYVLKTIRHRGVVSFVDLFEDNTYVYLVMEHHGSPWTASEKENKAPSFLKKLPIVNSLLQSTPKKPLLTGGLKLEMAPESRKGSLTIPPPMPGMMRRSSCDLFECIEQHSRLPEKYAKYVFAQLIDVVGCLHYNGFVHRDIKDENIVIDDKYRMKLIDFGSTYLFDYRQEPQSLNRFFGTLNFAAPELIKKGSYQPMAAEIWSLGILLSILVTGESFFHDTDAIKHYKASPPNNPVSESCMDLIFRCLAYKPEDRWNCFKIRTHPWLADCQWSWSDLPTDACTESIARVRKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3JQB6</accession>
    <name>E3JQB6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">tRNA(m1A58)-methyltransferase subunit TRM6</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_00436</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TRM6/GCD10 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178262">
        <property type="protein sequence ID" value="EFP74480.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003307486.2">
        <property type="nucleotide sequence ID" value="XM_003307438.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JQB6"/>
    <dbReference type="SMR" id="E3JQB6"/>
    <dbReference type="FunCoup" id="E3JQB6">
        <property type="interactions" value="518"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JQB6"/>
    <dbReference type="GeneID" id="10528040"/>
    <dbReference type="KEGG" id="pgr:PGTG_00436"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00436"/>
    <dbReference type="eggNOG" id="KOG1416">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_010916_1_0_1"/>
    <dbReference type="InParanoid" id="E3JQB6"/>
    <dbReference type="OrthoDB" id="10254665at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031515">
        <property type="term" value="C:tRNA (m1A) methyltransferase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017423">
        <property type="entry name" value="TRM6"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12945">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR EIF3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12945:SF0">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE NON-CATALYTIC SUBUNIT TRM6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04189">
        <property type="entry name" value="Gcd10p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="288"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="397"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Pro residues" evidence="7">
        <location>
            <begin position="292"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="7">
        <location>
            <begin position="397"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP74480.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="472" mass="52609" checksum="FA6C5551EA729193" modified="2012-04-18" version="2">MTRTISAGDNLLLKLPSGQTRTIKNLTADSSVSLGKFGKFQTNELINQPFGLTFEILEDGKLVLYDQSILAVEPNTTIDEMKSFESIKGMANGISNVEDIEATNELIKESDGAQKLTNQEIEELKKSGLSGREIILRQIQQHSAFELKSEFSKAKYIKRKEKKFMKMFTCIDPTIHNLSQYLFENHHFAVKGLRPDTLSQMLSLSNVRPGWKGIVIDEIGGLLVAAVLIRMGGQGTIFVINNADSPPDLHLLELFNLSPSTMKPLKSLNWAQIEADWTTNEIEELLSLHQGPPQPPPVLEPPTPLDPSVDSSSNRPKKEPNNRSKSKKKFERVQDLLNVRREFFETGFEGLITCSEYEPESIVTKLLTKLTGSSTIVIYSCHLRPLSDLQTLLKKSSVPSTSSSSSSSSSPGEQNELSRQMRETKTEFIQITISEPWLRAYQVLVGRTHPEMNGTHHGGFIFSAIKVTSSCS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="65" xmlns="https://uniprot.org/uniprot">
    <accession>E3JSB6</accession>
    <name>E3JSB6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">OTU domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_01434</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP74841.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319260.2">
        <property type="nucleotide sequence ID" value="XM_003319212.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JSB6"/>
    <dbReference type="FunCoup" id="E3JSB6">
        <property type="interactions" value="368"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JSB6"/>
    <dbReference type="GeneID" id="10546957"/>
    <dbReference type="KEGG" id="pgr:PGTG_01434"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01434"/>
    <dbReference type="HOGENOM" id="CLU_034963_2_0_1"/>
    <dbReference type="InParanoid" id="E3JSB6"/>
    <dbReference type="OrthoDB" id="415023at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004843">
        <property type="term" value="F:cysteine-type deubiquitinase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd22748">
        <property type="entry name" value="OTU_OTUD6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.70.80:FF:000025">
        <property type="entry name" value="Unplaced genomic scaffold supercont1.1, whole genome shotgun sequence"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.70.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003323">
        <property type="entry name" value="OTU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038765">
        <property type="entry name" value="Papain-like_cys_pep_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050704">
        <property type="entry name" value="Peptidase_C85-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419:SF10">
        <property type="entry name" value="DEUBIQUITINASE OTUD6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12419">
        <property type="entry name" value="OTU DOMAIN CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02338">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54001">
        <property type="entry name" value="Cysteine proteinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50802">
        <property type="entry name" value="OTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OTU" evidence="2">
        <location>
            <begin position="242"/>
            <end position="409"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="168"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="29"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="76"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="95"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="142"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="187"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50802"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP74841.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="410" mass="45572" checksum="0F4B4FE50834D1CF" modified="2012-04-18" version="2">MTNSNQAEHQQQQQQTNGAGEEKQQENGTTTAKTIKTAADILSRPRLPPKKKLSTSNKKPGKKNKNKNEQLVTATEKLEEKEEETEGHPTESIDNDQLLDQLLTQTNHLTLSSSEPTTPSTPTTTKTQQQQQQQQQQQQQQEANGNPSRSSTISRKLHGLSEDIKEVAHAVSSSINPSSPGKLASIKPKKKPHRTKEKIAKRQAAQLQAQLDAQLESSIHPVIDHRQLEAEALLRICLSLKLQIFEIIPDGHCLFSAIADQMNVLSGQTNGPSTATTTPTKGDEAVGGALRYTYQTCRKLASEYMRKHPEEFIHYLPGQDDGLEAGLMSSKEYEEHCDRVRDSACWGGEPEILALSKALKFSIHVIQAFHPTIKVSEEFLEPRGNIALTISYHRKSYGLGEHYNSLRPIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>E3JSM8</accession>
    <name>E3JSM8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_01646</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178263">
        <property type="protein sequence ID" value="EFP75053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319472.1">
        <property type="nucleotide sequence ID" value="XM_003319424.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JSM8"/>
    <dbReference type="GeneID" id="10547415"/>
    <dbReference type="KEGG" id="pgr:PGTG_01646"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_01646"/>
    <dbReference type="HOGENOM" id="CLU_006543_0_0_1"/>
    <dbReference type="InParanoid" id="E3JSM8"/>
    <dbReference type="OMA" id="NIEHRTI"/>
    <dbReference type="OrthoDB" id="10315539at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="1092"/>
            <end position="1111"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="201"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="322"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="416"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="506"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="607"/>
            <end position="638"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="668"/>
            <end position="704"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="790"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="852"/>
            <end position="1070"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="207"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="225"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="287"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="330"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="369"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="510"/>
            <end position="519"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="615"/>
            <end position="628"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="684"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="796"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="814"/>
            <end position="830"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="831"/>
            <end position="840"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="885"/>
            <end position="897"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="901"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="948"/>
            <end position="969"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="979"/>
            <end position="990"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="1020"/>
            <end position="1041"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP75053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1131" mass="124489" checksum="39648A24F1DDBC41" modified="2011-01-11" version="1">MFSQRHGGWRNLYFVILSACRIQCMYQIPHPSTGQLAAWASPPPEAEIQHFETGALLPWHQPGLDGRPTLVQGTPSATFSPYHHEAVPSVGDQAGRYVFVSPEPPAHPFQTLVPPTSQDTILHPHPSMVYVYPWSPQMLSQHPKYRRPVSGSSLNPHAAEFAPKPMFPPVAELTHLDGPNAVTEASHSDLNMGASIFSEGAAHQFPSPGNGQSLNVQASEVKTQPPATISAPITPAPTLKPAGNTGASSAPTGAGQIHPSEAKASPAQLSEAKSTSSTTSSIPKAATRQSSLAETESNLSESIKTFETKDLSKSINDKIIQQRVEHKKQQKVEDDEKGGSARLPPNPVDAIAKPKARQEELQPPNLQDDSGRTGVHDSTFDQPSKEERFDHQSTPPKEEDLEHKTRFLKKVLKIQPHIPNDKLSQKVTKAQDGNKDDGVSRQTLARTGKEGQREIDLQTEDQPHNTPRVDSTRTVLKTNAERLDTTSATPKKIESDRVNFRQKAPKDITSPLSPFSSSGKFEPAEIQGTPPTVLSDSQTEAEGVLENSPISLSDTRAFKMSQNKMVDRYDNEFPLLPLPAPVKEIDTVRTSNARPTTPPQTTIFLKKASQRSRKASYQASLNAQSTTKNTKRGDSDETAEAISLRKILDDKSDGLSEKTRLLLYKSIPDSSSSSTKAEMNRSSLKGKKSKISMKAHQPTAQVGKTESINSYPLRVKADVNLLTPASLSAKSVDRDSEKLLTDMQDTQIETLNPVPTTFPDLKRKGKRRCKFGTKGNFNRAMTTTRTTLIAPVGDGGKQKESFKKDPEALAQAKTPLTKNSPRQKKLPQSTEKIENKVSKNSERNVLEALIDPVAPKNLEKQPHGTKQSALQDDLPRMQIADTSGPLPQQSASDSNGLRNKAIVEDKEPNSNTSDKEMDATSSAKEVSDVTPVSRDGLNKSPVGFFDPSIRESAETTSKHQSAHFDREKQSFQPPSLNQSKDRKPSPKEMDATSSAKEVSDLTPVSRDGLNKSPVGFFDPSIRESAETTSKHQSAHFDREKQSFQPPSLNQSKDRKPSPQSTSRNSDIKEKFRQIRLPNYLPQHIQNPSEFPIPHYTIAWIQILILLCFRFCSKIGRKTVVHFTKPNVRLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>E3JTP6</accession>
    <name>E3JTP6_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RlpA-like protein double-psi beta-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_00770</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75439.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319858.2">
        <property type="nucleotide sequence ID" value="XM_003319810.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JTP6"/>
    <dbReference type="GeneID" id="10543067"/>
    <dbReference type="KEGG" id="pgr:PGTG_00770"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00770"/>
    <dbReference type="eggNOG" id="ENOG502S7JK">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_831786_0_0_1"/>
    <dbReference type="InParanoid" id="E3JTP6"/>
    <dbReference type="OrthoDB" id="406505at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd22191">
        <property type="entry name" value="DPBB_RlpA_EXP_N-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.10">
        <property type="entry name" value="RlpA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051477">
        <property type="entry name" value="Expansin_CellWall"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036908">
        <property type="entry name" value="RlpA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31836:SF22">
        <property type="entry name" value="RLPA-LIKE PROTEIN DOUBLE-PSI BETA-BARREL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50685">
        <property type="entry name" value="Barwin-like endoglucanases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="2">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003171520" description="RlpA-like protein double-psi beta-barrel domain-containing protein" evidence="2">
        <location>
            <begin position="29"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP75439.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="339" mass="37092" checksum="92B046E62435649B" modified="2012-04-18" version="2">MKTFNHGETKSTFASLASLLILIPAVCCSGKTSAHKLSKKQLPVHSEDWMILPSHGLMLTHYQTGPKHVNGVPVISRRDADISLFSSALSGLTSSQDDFLDWDAELVTDLKNAPLQDASSRDVVTRSGLVHITRNSQGKDAVRIEASPLLAKPLTLSPYNPNRTIKRFTQIKRSSKKQNVKLPKYNPKALSKSKGLQTLLNPTHPQLDVAKGAIIQTKITWYTGHDLLNPYCAQKSGWTPTDSSLIIAVTQEWKQRPKCGDFFEITVVDKHAGQVGKSVIARVVDLCGGCAPERAHADLSKAAFTKLFTLDVGVVSGLAMRKVAPPKKWNVALYGPRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>E3JU06</accession>
    <name>E3JU06_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">PGTG_00862</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="12" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75531.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319950.2">
        <property type="nucleotide sequence ID" value="XM_003319902.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JU06"/>
    <dbReference type="FunCoup" id="E3JU06">
        <property type="interactions" value="58"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JU06"/>
    <dbReference type="GeneID" id="10543247"/>
    <dbReference type="KEGG" id="pgr:PGTG_00862"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00862"/>
    <dbReference type="HOGENOM" id="CLU_048121_2_0_1"/>
    <dbReference type="InParanoid" id="E3JU06"/>
    <dbReference type="OrthoDB" id="272512at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016746">
        <property type="term" value="F:acyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063:SF60">
        <property type="entry name" value="LYSOPHOSPHATIDIC ACID:OLEOYL-COA ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23063">
        <property type="entry name" value="PHOSPHOLIPID ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="6" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="46"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="75"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="10">
        <location>
            <begin position="121"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="EFP75531.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="265" mass="29686" checksum="608F44F735AD3ECD" modified="2012-04-18" version="2">MEKFSTWRDPSTGLAPFVYPLPPLSTIQLPRIAWIPLVPLALFRTALIVVLGLLFVIFEGSGLVFSLFSVKLHSILQYYCTAIFSRITLYLLGFTQLPSDRPFTHRTSSQKLISTTVRPGDLVVCNWSSYVDILYLAYLYNPIFIIPVLKHGAPSQGSEVLVRGFESKGVLGMIFRTGHPPSHNPATKIKSLAQFLQESKRLRRPLIIFPEGTTSNNRALLRCPKLNEISVGKYDGKVKVFCLAIKHETPTPFKNSITVPIPTSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3JU38</accession>
    <name>E3JU38_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_00894</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178264">
        <property type="protein sequence ID" value="EFP75563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003319982.1">
        <property type="nucleotide sequence ID" value="XM_003319934.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JU38"/>
    <dbReference type="GeneID" id="10543279"/>
    <dbReference type="KEGG" id="pgr:PGTG_00894"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_00894"/>
    <dbReference type="HOGENOM" id="CLU_754669_0_0_1"/>
    <dbReference type="InParanoid" id="E3JU38"/>
    <dbReference type="OrthoDB" id="2498297at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="13"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="94"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="305"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="19"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="55"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP75563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="367" mass="40229" checksum="059EB87DA93944A1" modified="2011-01-11" version="1">MLDAFTRYSRSKLIRRDSGSSTHGSGTLKAQLSRRISSDEPGRTLESPEPSIATGSSAGSFLSSHSGDSQPSGPSLKQTLVKFRQQRLRPLSILAGNPKLVRNRRPSHSRESEESTDSGQFQVGAMGRILSSDEHSSHFIPRAALEQGGDYVIVHSEYAQALPNNLQPHASMPEISSNKKPWSYNLESLPCGIRPSEKLVVEEESDGSLDDEMLDTLCTLRYTIQQIKDHRRQSPKSNPLVKCDSLPTFGLTSTGHQNLLRTLSVPSRFIEDKAASFDLMNGSGLPYCLVSPAHLLNQLPSIHAGNHSTPASRDLPNPGMIENEDEGDDTSFCTLPEEEPDYQSGPYVLPIWPNLPSALQPAFDPKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXG9</accession>
    <name>E3JXG9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Transcription initiation factor TFIID subunit 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_02205</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TAF9 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321163.1">
        <property type="nucleotide sequence ID" value="XM_003321115.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXG9"/>
    <dbReference type="FunCoup" id="E3JXG9">
        <property type="interactions" value="50"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JXG9"/>
    <dbReference type="GeneID" id="10528765"/>
    <dbReference type="KEGG" id="pgr:PGTG_02205"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02205"/>
    <dbReference type="eggNOG" id="KOG3334">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_068315_5_0_1"/>
    <dbReference type="InParanoid" id="E3JXG9"/>
    <dbReference type="OMA" id="WNMKDEW"/>
    <dbReference type="OrthoDB" id="341924at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000124">
        <property type="term" value="C:SAGA complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005669">
        <property type="term" value="C:transcription factor TFIID complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003713">
        <property type="term" value="F:transcription coactivator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051123">
        <property type="term" value="P:RNA polymerase II preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07979">
        <property type="entry name" value="HFD_TAF9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003162">
        <property type="entry name" value="TFIID-31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051431">
        <property type="entry name" value="TFIID_subunit_9"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48068">
        <property type="entry name" value="TAF9 RNA POLYMERASE II, TATA BOX-BINDING PROTEIN (TBP)-ASSOCIATED FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48068:SF4">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN ASSOCIATED FACTOR 9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02291">
        <property type="entry name" value="TFIID-31kDa"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0539">Nucleus</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0805">Transcription regulation</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="128"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="6">
        <location>
            <begin position="174"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007646"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP76744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="222" mass="23982" checksum="E3514F8FAF86989A" modified="2011-01-11" version="1">MSTPANNPNAEGATSSAPEPSIRQLPVPSTPRDARIIALLLASMGVQSASEGVVRVLMEFAHRYTVDILSDALVYAEHAGRAHAAHGPDVEDVRLAVQAKVEHSMSGPMSKEFLLNLADNLNRTALPPIPERYGIRLPPEKDRLTAPNFDIAPKPPPTEVESPSDHEEGMFGGIDDDDSSDSDSDSDTEMDFAPVISINGNGNLASDQARRRLEDVEEDDYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXJ2</accession>
    <name>E3JXJ2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Transcription elongation regulator 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_02228</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76767.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321186.2">
        <property type="nucleotide sequence ID" value="XM_003321138.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXJ2"/>
    <dbReference type="FunCoup" id="E3JXJ2">
        <property type="interactions" value="153"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JXJ2"/>
    <dbReference type="GeneID" id="10528789"/>
    <dbReference type="KEGG" id="pgr:PGTG_02228"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02228"/>
    <dbReference type="eggNOG" id="KOG0155">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_013872_0_0_1"/>
    <dbReference type="InParanoid" id="E3JXJ2"/>
    <dbReference type="OrthoDB" id="410044at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070063">
        <property type="term" value="F:RNA polymerase binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd00201">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.440:FF:000048">
        <property type="entry name" value="Conserved expressed protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.440:FF:000044">
        <property type="entry name" value="Transcription elongation regulator 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.440">
        <property type="entry name" value="FF domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002713">
        <property type="entry name" value="FF_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036517">
        <property type="entry name" value="FF_domain_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045148">
        <property type="entry name" value="TCRG1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001202">
        <property type="entry name" value="WW_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036020">
        <property type="entry name" value="WW_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15377">
        <property type="entry name" value="TRANSCRIPTION ELONGATION REGULATOR 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15377:SF3">
        <property type="entry name" value="WW DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01846">
        <property type="entry name" value="FF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00397">
        <property type="entry name" value="WW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00441">
        <property type="entry name" value="FF"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00456">
        <property type="entry name" value="WW"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81698">
        <property type="entry name" value="FF domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51045">
        <property type="entry name" value="WW domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51676">
        <property type="entry name" value="FF"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01159">
        <property type="entry name" value="WW_DOMAIN_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50020">
        <property type="entry name" value="WW_DOMAIN_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="WW" evidence="4">
        <location>
            <begin position="17"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="domain" description="WW" evidence="4">
        <location>
            <begin position="108"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="domain" description="FF" evidence="5">
        <location>
            <begin position="304"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="domain" description="FF" evidence="5">
        <location>
            <begin position="372"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="60"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="204"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="259"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="481"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="143"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="61"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="76"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="259"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="496"/>
            <end position="511"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="512"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP76767.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="584" mass="66424" checksum="611F4F261C02AF12" modified="2012-04-18" version="2">MASPSPTEVSVPIYAAAPLPPGWTEHRAPTGQFYYYHAESGESTYVRPSIPVLATKEIDTNVVSTSSNKSGEGTEKNASTTSTTSTTTTTKKKKEKPKTKEAIPETVWMKVTTTAGNIFYTNVETKVSSWTIPEEIKEQVKLYEEAQARKAEELRLAEVEQARAKAEEAQRIHEAEQRRMIELEVQKVRAEVEAEAQLRGLKRKNDHAAEPGQPPVQTPANPSTGSEGDRLAKIAKVEQTAQPEDDQDEDWQRQIAEEMAQEAAEQSGTSIPHQTTTPTPTPTTVASNPPPATINQPPLTGLSLEELKATFKAMLLEKSIDPMAPWDNELPKFVTDARYLALPSMKERRDLFDEFCKEKIRQLRAAKSAVPKVDPPQAYRSLLIEFVTSTRTLWEDFKSKHKKDPRFRNFGRDDREREKVFKSWLKELGEQKRKLLLKAEDDFKKLLIEKGIPPKEGDEEEEKEEEYKDFKERVKNDPRFLAITSNSSKESMWKTWRTDQRRARDLSKNDEGATNVSTQNGKANPEEEEKEEEENKKKKMEKQMASLKAREDHVNQIKKKIDKEAAVNRALVDKDHFGINPETH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXP9</accession>
    <name>E3JXP9_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_02285</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321243.1">
        <property type="nucleotide sequence ID" value="XM_003321195.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXP9"/>
    <dbReference type="GeneID" id="10528927"/>
    <dbReference type="KEGG" id="pgr:PGTG_02285"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02285"/>
    <dbReference type="HOGENOM" id="CLU_054808_0_0_1"/>
    <dbReference type="InParanoid" id="E3JXP9"/>
    <dbReference type="OrthoDB" id="2501708at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="248"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="290"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="1"/>
            <end position="12"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="16"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="42"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="248"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="261"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="305"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="326"/>
            <end position="341"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="348"/>
            <end position="372"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP76824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="416" mass="47663" checksum="E8081A25866AE8F3" modified="2011-01-11" version="1">MPSSKSSSKSTSNHPGSDHSNSKPSKHQVDKNSHRHRRHSHGEHVPNTHPDDNLELPEHTNSTAQHSQKSKSKDDHGQELYRLDEGSQIWRTYDGSVIYWEVHKLTYQELSEAIWNWAERGLEPTQKLYMASLRPPHGGHRSGLLDFFCSHRQKLVRDHIQRIYSDLYLILRTWTELKHYLQQLNEKSSPQERSQTLKKIENRLTFHLMVALREYCGLPLDPKHPDKPSWPATGDKDLPTTRLEPGALASASNSVSASSIKSEKSSPRTHDFDTKAICCHCHQILDHRETEETLKQDTKLSIPPSGSTSKQESSRSRSQTSRSSSRKADNHSKSRKVNEHRSKSRSRKDRENKPEDKDSKGKDTPHSSHDLANKNSKSTRSRHSEEKSNDDSEDSSLISFDSTQFSNAFGMIFHHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="70" xmlns="https://uniprot.org/uniprot">
    <accession>E3JXX4</accession>
    <name>E3JXX4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">High-affinity Zn(2+) transporter zrt1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">PGTG_02360</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="11" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the ZIP transporter (TC 2.A.5) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <dbReference type="EMBL" id="DS178266">
        <property type="protein sequence ID" value="EFP76899.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321318.2">
        <property type="nucleotide sequence ID" value="XM_003321270.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JXX4"/>
    <dbReference type="FunCoup" id="E3JXX4">
        <property type="interactions" value="233"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JXX4"/>
    <dbReference type="GeneID" id="10529084"/>
    <dbReference type="KEGG" id="pgr:PGTG_02360"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_02360"/>
    <dbReference type="eggNOG" id="KOG1558">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="InParanoid" id="E3JXX4"/>
    <dbReference type="OrthoDB" id="2502063at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005385">
        <property type="term" value="F:zinc ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071577">
        <property type="term" value="P:zinc ion transmembrane transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004698">
        <property type="entry name" value="Zn/Fe_permease_fun/pln"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00820">
        <property type="entry name" value="zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040:SF32">
        <property type="entry name" value="ZINC-REGULATED TRANSPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11040">
        <property type="entry name" value="ZINC/IRON TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="32"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="106"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="261"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="303"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="337"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006939"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU362088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="EFP76899.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="358" mass="38330" checksum="F97809A4104100DB" modified="2012-04-18" version="2">MSSLDNSTTTTASVEDECSNIAPINEQLPLRIAAIFIILVTSMLGTLFPVLTRQSKRLSVSPWIYEAVKYFGSGVILATALIHLLAPANKALSSPCLSVGWSLYPFSQGITLASIFVIFIIEIIAIRVGTSRLAALGLKYCAHGIGADQPPTHEATAPSASGAHHTHDTNDRLESKLDKLSEETVATPACLPSAEVGSQLIGAAILELGVIFHSVVIGLTLAVNAQFTTFFLVIIFHQMFEGLGLGARLSQLSLPTRYRRLPLWASLLYSFVTPLGLTIGLGLRNTYNPNSATALMVSGCLDSFSAGILLYTGLVELLAHDFVFNKTLLLEHSNCRLTFDIVCVVSGAGLMALLGRWA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3JYE0</accession>
    <name>E3JYE0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_03021</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003321436.1"/>
    </dbReference>
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    <dbReference type="STRING" id="418459.E3JYE0"/>
    <dbReference type="GeneID" id="10529412"/>
    <dbReference type="KEGG" id="pgr:PGTG_03021"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03021"/>
    <dbReference type="eggNOG" id="KOG1836">
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    <dbReference type="InParanoid" id="E3JYE0"/>
    <dbReference type="OrthoDB" id="10255630at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="86"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="169"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP77065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="189" mass="21196" checksum="DA6500689095FD5E" modified="2011-01-11" version="1">MVSELRRELEGLRVELLATLEERKTAVDQTHVDSIIQQHQIDLLTTWSQIRALESRVFEEESHSLGMLNRVENLQDELRGAKEALRQRKNTPLTDSPNPAIPSSSSFAVPQATVGSSSETCPQSTSKKTNFLKMIDESNLSEQTKQTRTKSLNLLKTQMENDLGLLNLSSNALSHHPPDPSNAHSLKNW</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>E3JYI7</accession>
    <name>E3JYI7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_03068</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
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        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
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            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77112.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321531.2">
        <property type="nucleotide sequence ID" value="XM_003321483.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JYI7"/>
    <dbReference type="GeneID" id="10537323"/>
    <dbReference type="KEGG" id="pgr:PGTG_03068"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03068"/>
    <dbReference type="HOGENOM" id="CLU_372602_0_0_1"/>
    <dbReference type="InParanoid" id="E3JYI7"/>
    <dbReference type="OrthoDB" id="2502708at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46563:SF4">
        <property type="entry name" value="ASPARTYL_ASPARAGINYL BETA-HYDROXYLASE ISOFORM X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46563">
        <property type="entry name" value="RING-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="84"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="285"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="548"/>
            <end position="581"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="686"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="181"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="10"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="85"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="95"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="300"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="325"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="690"/>
            <end position="732"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP77112.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="744" mass="82127" checksum="C9FF872FE48D3511" modified="2012-04-18" version="2">MTNIDPSLPTNNNNNNSSNNPSSTVNSNTNNNNNNNNTNHNRKRSRQSSSEDLLSTDHDLSSLDPTLSAINDSMAADTANLLNHHSQHTPKRKRDSSDQLRQSDLDHHQDNNSNNRPSLDTLQLAHLITSIQAHQQPPSSSVPIEPSSSSHLAGYNILPDYLLPSEPTIEPEWKEKFEIVLNPLKLEISRLRSRIEILEDERLVMAREITSLKGSHNIPSDLPPRSFPGSTHQIPLPAARDNPRMTTILGNSTLHNHALDIPVGPLPEIGSSSQAHSTAGLNHQLAASASSGTKPVIPQSVINSSSKFETGTSFRRQSTRKKSEAPSSFNTSPASNATQASNNRSKHRKPELSRTVRATVFRLMGVSSSQSPVPSEAGLPSETCSKFGNEKSRAYHGYTTSPCFPEYTPDELYDPVTKARIWRWDWSKTIRQSQNNTNFAKEIRNVIAREAADPIRPMHTEVPCSDWLYLDDAIDSAYTNMRRERENLIDPTKMVKKEVHRARNKKRGLKEDKYKRRRKALDEYRADPQSFGQRLREANLRMTEKPILGDGLVGEEEQSGRGQRSGETEDGAGRAAAAAEEEERWDDALDIKYMSSEDEGHVNDFENPIGIGTEKTDNTLAASDKVFAICRPTWRSDQLHNLFSVLDIIKQPERAYRRVLGPYRDTRPPLGTPSWMINPACEASNPLGPSASSSSTTTTSAAAAVAAPSNSRSLPLPSTSTSASASASDTALVNHDASLTAQKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="61" xmlns="https://uniprot.org/uniprot">
    <accession>E3JZB5</accession>
    <name>E3JZB5_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase</fullName>
            <ecNumber evidence="3 14">2.4.1.258</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Dol-P-Man-dependent alpha(1-3)-mannosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGTG_03346</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11 14">Dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase that operates in the biosynthetic pathway of dolichol-linked oligosaccharides, the glycan precursors employed in protein asparagine (N)-glycosylation. The assembly of dolichol-linked oligosaccharides begins on the cytosolic side of the endoplasmic reticulum membrane and finishes in its lumen. The sequential addition of sugars to dolichol pyrophosphate produces dolichol-linked oligosaccharides containing fourteen sugars, including two GlcNAcs, nine mannoses and three glucoses. Once assembled, the oligosaccharide is transferred from the lipid to nascent proteins by oligosaccharyltransferases. In the lumen of the endoplasmic reticulum, adds the first dolichyl beta-D-mannosyl phosphate derived mannose in an alpha-1,3 linkage to Man(5)GlcNAc(2)-PP-dolichol to produce Man(6)GlcNAc(2)-PP-dolichol.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl beta-D-mannosyl phosphate = an alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;2)-alpha-D-Man-(1-&gt;3)-[alpha-D-Man-(1-&gt;3)-alpha-D-Man-(1-&gt;6)]-beta-D-Man-(1-&gt;4)-beta-D-GlcNAc-(1-&gt;4)-alpha-D-GlcNAc-diphospho-di-trans,poly-cis-dolichol + a di-trans,poly-cis-dolichyl phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29527"/>
            <dbReference type="Rhea" id="RHEA-COMP:19498"/>
            <dbReference type="Rhea" id="RHEA-COMP:19501"/>
            <dbReference type="Rhea" id="RHEA-COMP:19516"/>
            <dbReference type="Rhea" id="RHEA-COMP:19517"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57683"/>
            <dbReference type="ChEBI" id="CHEBI:58211"/>
            <dbReference type="ChEBI" id="CHEBI:132515"/>
            <dbReference type="ChEBI" id="CHEBI:132516"/>
            <dbReference type="EC" id="2.4.1.258"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12 14">
            <dbReference type="Rhea" id="RHEA:29528"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Endoplasmic reticulum membrane</location>
            <topology evidence="1 14">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the glycosyltransferase ALG3 family.</text>
    </comment>
    <dbReference type="EC" id="2.4.1.258" evidence="3 14"/>
    <dbReference type="EMBL" id="DS178267">
        <property type="protein sequence ID" value="EFP77390.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003321809.2">
        <property type="nucleotide sequence ID" value="XM_003321761.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3JZB5"/>
    <dbReference type="FunCoup" id="E3JZB5">
        <property type="interactions" value="469"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3JZB5"/>
    <dbReference type="GeneID" id="10538100"/>
    <dbReference type="KEGG" id="pgr:PGTG_03346"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03346"/>
    <dbReference type="eggNOG" id="KOG2762">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_035382_1_1_1"/>
    <dbReference type="InParanoid" id="E3JZB5"/>
    <dbReference type="OrthoDB" id="20028at2759"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052925">
        <property type="term" value="F:dol-P-Man:Man(5)GlcNAc(2)-PP-Dol alpha-1,3-mannosyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007873">
        <property type="entry name" value="Glycosyltransferase_ALG3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12646:SF0">
        <property type="entry name" value="DOL-P-MAN:MAN(5)GLCNAC(2)-PP-DOL ALPHA-1,3-MANNOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12646">
        <property type="entry name" value="NOT56 - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05208">
        <property type="entry name" value="ALG3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0256">Endoplasmic reticulum</keyword>
    <keyword evidence="5 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="10 14" id="KW-0472">Membrane</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 14" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 14" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="21"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="103"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="177"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="213"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="14">
        <location>
            <begin position="263"/>
            <end position="285"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00015561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00093457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU364047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EFP77390.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="425" mass="49281" checksum="61BA1A94E2B84BF4" modified="2012-04-18" version="2">MSDHLKATLKYCRRILVSKEQYDLIAGLVLLSELVLNTAIIKYVRYTEIDWIAYMQQIRSYSLGERNYLKLKGDTGPLVYPAGFVHVYSVLYSLTQQGQDVKLAQYLFGAIYLITMGFVLAIYKRCESLPTYAIFSLILSKRMHSIYVLRLFNDGIAMMGLYVSIWCLVTQRWNWSALWFSLALSIKMNILLFFPALGFIYFQTVGVLKTINLMLIIVLVQAWLAIPFLLEFPREYLSKAFEFKRVFEYQWTVNWRMVPQSTFLSSGFASSLTLLHLSTLVWFAWTRWSRASRGDSLISLLVRGLRNWNRPAAIHPDQLTPDYIVKVCFTSNLIGIICARTLHYQFYSWYAHQIVFLAWQTPYPIFLRILLMLSIEYSWNVFPSTSISSTVLLLSNTSLLVGTWWSFDQSCSATPVAVKKKHKVT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3K0H8</accession>
    <name>E3K0H8_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Saccharopine dehydrogenase NADP binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_03759</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the saccharopine dehydrogenase family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178268">
        <property type="protein sequence ID" value="EFP77803.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003322222.2">
        <property type="nucleotide sequence ID" value="XM_003322174.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K0H8"/>
    <dbReference type="GeneID" id="10540625"/>
    <dbReference type="KEGG" id="pgr:PGTG_03759"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_03759"/>
    <dbReference type="eggNOG" id="KOG2733">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_031002_0_2_1"/>
    <dbReference type="InParanoid" id="E3K0H8"/>
    <dbReference type="OrthoDB" id="10268090at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005811">
        <property type="term" value="C:lipid droplet"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="GOC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009247">
        <property type="term" value="P:glycolipid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000413">
        <property type="entry name" value="Trans-acting enoyl reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051276">
        <property type="entry name" value="Saccharopine_DH-like_oxidrdct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005097">
        <property type="entry name" value="Sacchrp_dh_NADP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12286">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12286:SF5">
        <property type="entry name" value="SACCHAROPINE DEHYDROGENASE-LIKE OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03435">
        <property type="entry name" value="Sacchrp_dh_NADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Saccharopine dehydrogenase NADP binding" evidence="2">
        <location>
            <begin position="10"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00038048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP77803.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="424" mass="46323" checksum="670D18F73C891ACC" modified="2012-04-18" version="2">MLHTDRNIDIIVYGATGFTGKLVCQHLQRRYLDPSAEEKLNWAIGGRSQEKLDKVKSELGLPKDLKVFVADSNDEKALNEMCKQSKSLVTLVGPYAMYGDKLVAACVANGTHYFDLTGETLWASRQISKLESQARESKTIVVHSCGYDSVPSDLNAMLAVRELKKVAGQDVKVGRVTAGATAKGGVSGGTIASMLNMYDEGVSQLRKAMSCYLLSPVKGHQKKDPSWVTKEPGLVGGFFIMAPHNGAIVRRSWGLLEASSDSAIKNERYGAKFNYDEYLITPGQVAGFLLTLFFRVFALMMLVPQARSLVKKYGTQPGDGPDEITRKNGHFKHITTAHSVEDDVQVRVTMSGNLDPGYGWTAISIAECAVTCVRAHNKLPPLAHQGGFLTPSTALGDALLERLELTGTTKIDKQVLRNDRKKTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>E3K3P4</accession>
    <name>E3K3P4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phospholipid/glycerol acyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_04652</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178271">
        <property type="protein sequence ID" value="EFP78696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003323115.1">
        <property type="nucleotide sequence ID" value="XM_003323067.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K3P4"/>
    <dbReference type="FunCoup" id="E3K3P4">
        <property type="interactions" value="75"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K3P4"/>
    <dbReference type="GeneID" id="10536633"/>
    <dbReference type="KEGG" id="pgr:PGTG_04652"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_04652"/>
    <dbReference type="HOGENOM" id="CLU_007860_2_0_1"/>
    <dbReference type="InParanoid" id="E3K3P4"/>
    <dbReference type="OMA" id="YTHFKDD"/>
    <dbReference type="OrthoDB" id="2427554at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004366">
        <property type="term" value="F:glycerol-3-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016287">
        <property type="term" value="F:glycerone-phosphate O-acyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd07992">
        <property type="entry name" value="LPLAT_AAK14816-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052744">
        <property type="entry name" value="GPAT/DAPAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002123">
        <property type="entry name" value="Plipid/glycerol_acylTrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31605">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31605:SF0">
        <property type="entry name" value="GLYCEROL-3-PHOSPHATE O-ACYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00563">
        <property type="entry name" value="PlsC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69593">
        <property type="entry name" value="Glycerol-3-phosphate (1)-acyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="177"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="583"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="644"/>
            <end position="666"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="675"/>
            <end position="695"/>
        </location>
    </feature>
    <feature type="domain" description="Phospholipid/glycerol acyltransferase" evidence="4">
        <location>
            <begin position="217"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="1"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="809"/>
            <end position="918"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="715"/>
            <end position="742"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="2">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="2">
        <location>
            <begin position="870"/>
            <end position="879"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP78696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="918" mass="102520" checksum="38AD14ED60EDC91B" modified="2011-01-11" version="1">MNQTNNSSNSSSNSHEHELDSTRTTTHHHHHPPKREELPLNNCITQVPTPPDTPNLTDSDQELQPQPQPHPNSPSINNTHSLISPPPPPLQLAISNVHSDPIHLAHYSPINQPTPAPPKLLNHTLQDTLEDLSSLLQTFRKILPLSHRLAQFVPRLIHRFSRSCVQQMAQMLYNRNLLASNVAYDLAILLLKWIINLFFREIRPRGSHKIPDKGPLIFVAAPHHNQFLDPVILMSEVRAAESARRVSFLTAEKSMKRPFIGLIARVMQSISVTRATDDAKDGTGRIFLSSKQTDPCILHGKNTRFTHELAPKKQILLSKQLGFVTVEVVEILDDCCLRVKKPFPTKGVEALREEGNKIEAGIDSDSVVGVPFKILPYVDQTQMYGKVFEKLKNGGCLGIFPEGGSHDRTDLLPLKAGVSIMALGAMSSNPEITVRIVPVGLSYFHPHRFRSRAVVEFGTPIEIPQELVRDFEKGDDHKKAAIGKVMELVYDGLKSVTVQAPDYDTLQVIQAARRLYRPPDRHVSLSTVVELNKRFITGYLRYKDEPRVMALKSKTLEYNKSISYLGLKDHQVEKVNRPWWKSLILLCFRAGLVTGWGVLALPGVILNSPMFICASIVSRKKAREALAASSVKIAGRDVLATWKVLVSLGLAPPLYIIYVAVAIWLAVKYEVSRKYVMWIPMGMLFALPSIGYSALKFGEVGMDVYKSLRPLLLSLLGDQNQLERVRKMRSELQAELHSVIEEYAPKIWENFEQTRIHTPSADVAPLSGDGRKSLGLGYGGRGVDLINHPLSWLDERLFGWSQYDSRPSQQKRRRLRRLKSEPTLAGERDEFSTSEEATTTDSGADEDGDYDSVVGMLSRFTGAGTGSGSRSRRTSRGSRSRSQLNLTELVTTDDTTTTGSSAAVKLGMTGDLNRRRKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3K599</accession>
    <name>E3K599_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_06016</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79695.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324114.2">
        <property type="nucleotide sequence ID" value="XM_003324066.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K599"/>
    <dbReference type="GeneID" id="10532894"/>
    <dbReference type="KEGG" id="pgr:PGTG_06016"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06016"/>
    <dbReference type="HOGENOM" id="CLU_2098029_0_0_1"/>
    <dbReference type="InParanoid" id="E3K599"/>
    <dbReference type="OrthoDB" id="10523704at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79695.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="116" mass="13375" checksum="6994C4A6D9B6AE71" modified="2012-04-18" version="2">MNTRDEKMIEFLPQAGKSKHVDEGMESHGGNHNSWESFDPMEIEIGINERMSKVPKESTINIGINAPHSRKITLEDTEESIKEMSLEEFVEMAKVVFGLKADEMDSLKNHLRYYFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="77" xmlns="https://uniprot.org/uniprot">
    <accession>E3K5A3</accession>
    <name>E3K5A3_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Acyl-CoA desaturase</fullName>
            <ecNumber evidence="16">1.14.19.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">PGTG_06020</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="21" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Stearoyl-CoA desaturase that utilizes O(2) and electrons from reduced cytochrome b5 to introduce the first double bond into saturated fatty acyl-CoA substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>octadecanoyl-CoA + 2 Fe(II)-[cytochrome b5] + O2 + 2 H(+) = (9Z)-octadecenoyl-CoA + 2 Fe(III)-[cytochrome b5] + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:19721"/>
            <dbReference type="Rhea" id="RHEA-COMP:10438"/>
            <dbReference type="Rhea" id="RHEA-COMP:10439"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15379"/>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
            <dbReference type="ChEBI" id="CHEBI:57387"/>
            <dbReference type="ChEBI" id="CHEBI:57394"/>
            <dbReference type="EC" id="1.14.19.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="16">Expected to bind 2 Fe(2+) ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the fatty acid desaturase type 1 family.</text>
    </comment>
    <dbReference type="EC" id="1.14.19.1" evidence="16"/>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324118.1">
        <property type="nucleotide sequence ID" value="XM_003324070.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K5A3"/>
    <dbReference type="FunCoup" id="E3K5A3">
        <property type="interactions" value="168"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K5A3"/>
    <dbReference type="GeneID" id="10532898"/>
    <dbReference type="KEGG" id="pgr:PGTG_06020"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06020"/>
    <dbReference type="eggNOG" id="KOG0537">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG1600">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_027359_3_1_1"/>
    <dbReference type="InParanoid" id="E3K5A3"/>
    <dbReference type="OMA" id="WWSSKHR"/>
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    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005789">
        <property type="term" value="C:endoplasmic reticulum membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:heme binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
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        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
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        <property type="term" value="F:stearoyl-CoA 9-desaturase activity"/>
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        <property type="project" value="GO_Central"/>
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        <property type="term" value="P:unsaturated fatty acid biosynthetic process"/>
        <property type="evidence" value="ECO:0000318"/>
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    </dbReference>
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        <property type="entry name" value="Delta9-FADS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acyl-CoA desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Acyl-CoA_deSatase_haem/ster-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015876">
        <property type="entry name" value="Acyl-CoA_DS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001199">
        <property type="entry name" value="Cyt_B5-like_heme/steroid-bd"/>
    </dbReference>
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        <property type="entry name" value="Cyt_B5-like_heme/steroid_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018506">
        <property type="entry name" value="Cyt_B5_heme-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005804">
        <property type="entry name" value="FA_desaturase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351">
        <property type="entry name" value="ACYL-COA DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11351:SF31">
        <property type="entry name" value="DESATURASE 1, ISOFORM A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00173">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00487">
        <property type="entry name" value="FA_desaturase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000345">
        <property type="entry name" value="OLE1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00075">
        <property type="entry name" value="FACDDSATRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01117">
        <property type="entry name" value="Cyt-b5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55856">
        <property type="entry name" value="Cytochrome b5-like heme/steroid binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00191">
        <property type="entry name" value="CYTOCHROME_B5_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50255">
        <property type="entry name" value="CYTOCHROME_B5_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0249">Electron transport</keyword>
    <keyword evidence="15 16" id="KW-0275">Fatty acid biosynthesis</keyword>
    <keyword evidence="8 16" id="KW-0276">Fatty acid metabolism</keyword>
    <keyword evidence="5 16" id="KW-0349">Heme</keyword>
    <keyword evidence="12 16" id="KW-0408">Iron</keyword>
    <keyword evidence="4 16" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="13 16" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 18" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 16" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="226"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="Cytochrome b5 heme-binding" evidence="19">
        <location>
            <begin position="380"/>
            <end position="460"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="17">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="17">
        <location>
            <begin position="1"/>
            <end position="16"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="17">
        <location>
            <begin position="47"/>
            <end position="61"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022617"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022832"/>
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    </evidence>
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    </evidence>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRNR" id="PIRNR000345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="EFP79699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="507" mass="57831" checksum="CAE3F7B1FEFE68E0" modified="2011-01-11" version="1">MSKPTLTIPTSPQNLRNRQRKNLPDYDPDSDLSESEGLGGTRPQPDDTWEDDEETAVDDDSYVQRTLRREKPLPPITWRNFYREINLISTLALTIVPVLSLYGALTTPLCRFTLAWSIVYYYFTGLGITAGYHRLWAHRSYNASPLLQYFLALGGSGAVEGSIRWWARGHRAHHRYTDTDLDPYSAHKGLLWSHVGWMIVKPRRKPGIADVSDLSRNQVVRWQHRWYLPLIFGMGFFFPTLVAGLGWGDWRGGFFYAGAARLLFVHHSTFCVNSLAHWLGEAPFDDKHTPRDHIITAFVTIGEGYHNFHHEFPQDFRNAIRWYQYDPTKWFIAVAAFLGLATELKTFPDNEIRKGQYGMKLKELQQDFREVKWPTSSNDLPILTWEQFVDEADKKNGRNLIVIGGFIHDVTDFIDEHPGGRALIKTRLGKDATTAFHGGVYEHSNAAHNLLAMLRVGVIEGGYEVEHLKKKVGVFRKEQQIPICGPKTLGTVSTPDSPVLGIKPIYP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3K671</accession>
    <name>E3K671_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05963</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178273">
        <property type="protein sequence ID" value="EFP79642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003324013.2"/>
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    <dbReference type="AlphaFoldDB" id="E3K671"/>
    <dbReference type="GeneID" id="10532840"/>
    <dbReference type="KEGG" id="pgr:PGTG_05963"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05963"/>
    <dbReference type="HOGENOM" id="CLU_2251383_0_0_1"/>
    <dbReference type="InParanoid" id="E3K671"/>
    <dbReference type="OrthoDB" id="10479038at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="84"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="104" mass="11670" checksum="0F833D9FBA052081" modified="2011-01-11" version="1">MTAGSKRKGLLGIADPNKAQVRGAWFVKSGAYKPFQALEFCGLVLTYQPRSVFRHDRLAIAQRILDSQLLSGWLSHGEAPQDRQWQNKLQHTEAQNQIIDAPQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>E3K6E3</accession>
    <name>E3K6E3_PUCGT</name>
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        <recommendedName>
            <fullName evidence="2">No apical meristem-associated C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_05099</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP79874.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003324245.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K6E3"/>
    <dbReference type="STRING" id="418459.E3K6E3"/>
    <dbReference type="GeneID" id="10533565"/>
    <dbReference type="KEGG" id="pgr:PGTG_05099"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05099"/>
    <dbReference type="HOGENOM" id="CLU_012390_7_4_1"/>
    <dbReference type="InParanoid" id="E3K6E3"/>
    <dbReference type="OrthoDB" id="10400817at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029466">
        <property type="entry name" value="NAM-associated_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125">
        <property type="entry name" value="F21J9.4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45125:SF3">
        <property type="entry name" value="NO-APICAL-MERISTEM-ASSOCIATED CARBOXY-TERMINAL DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14303">
        <property type="entry name" value="NAM-associated"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
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        <location>
            <begin position="266"/>
            <end position="411"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="296"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="413"/>
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        </location>
    </feature>
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        <location>
            <begin position="13"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="25"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="36"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
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            <begin position="121"/>
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        </location>
    </feature>
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        </location>
    </feature>
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        </location>
    </feature>
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        <location>
            <begin position="475"/>
            <end position="508"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF14303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP79874.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="508" mass="57716" checksum="D8D9A81F602A2F56" modified="2012-04-18" version="2">MEYSIDPALGPLNDTNIQATNKASHPLEVDSDTEKRPKKKNKKTKKAPTIESTLPPKKNIPTAKNDALPAKKKLIPAKKNPQPALRNGASLASTNATAKKMVSSEGGDVPAEKICPIKQSQPEKKNAPAEIQVEKITPKNKKKNVSKEAATSESDTKPSRAKAYQEDEDVQLCKSWLEVSQDPLNSTNQAANTFWNRIADHFQAAMKDKSRTSVSIKSRWQLLQRRINKFCGCIQQVEQANQSGTNVEDRLSSALKLYLALSESSFTHLRCYNILCHAQKWKDHCVKLEKKPVEHCSTPSVDNTTNQSLSSDAPAMDENSDASTIQSGRTARPIGNKKAKEFALKAREDKKFKDDIIAVHKELAQQTQVQNNILSEQRDSISMLADEAVMKADLSSVSETSRPYYEWQQKKVLDKMKREQEEYEKKETDRKKREEEAKKKEIEEAAKKKEKEEEEKKKKDEEIRKKSAVIILLDDHDEEEYDDDNDDEGEEEEDEDEEEEEEEEEEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3K6G7</accession>
    <name>E3K6G7_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_05114</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178274">
        <property type="protein sequence ID" value="EFP79889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324308.1">
        <property type="nucleotide sequence ID" value="XM_003324260.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K6G7"/>
    <dbReference type="STRING" id="418459.E3K6G7"/>
    <dbReference type="GeneID" id="10533580"/>
    <dbReference type="KEGG" id="pgr:PGTG_05114"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_05114"/>
    <dbReference type="HOGENOM" id="CLU_1415805_0_0_1"/>
    <dbReference type="InParanoid" id="E3K6G7"/>
    <dbReference type="OrthoDB" id="166375at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="141"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="21"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="44"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="143"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP79889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="192" mass="20740" checksum="C5A5321A24B92D87" modified="2011-01-11" version="1">MSLHKSQATTLLHPPPPPRPQSSSSTTASPVCTTIIDDHQKDGDENEEDEEEEELTTEVGSLDGTRSSDIYYHSLNIIVLSRTMNRKANCEEIRRAEVAASELGRQQMKVLEAGAAVRLDPSARVKINPELTCEARLLTPSQAAKPSASGTPSSSTASSAITNNATKEKTQLSKFEELVASQVHVDINIVDS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3K826</accession>
    <name>E3K826_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">60S acidic ribosomal protein P0</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">PGTG_06188</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="8" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. uL10 forms part of the P stalk that participates in recruiting G proteins to the ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80232.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324651.2">
        <property type="nucleotide sequence ID" value="XM_003324603.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K826"/>
    <dbReference type="FunCoup" id="E3K826">
        <property type="interactions" value="430"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K826"/>
    <dbReference type="GeneID" id="10534574"/>
    <dbReference type="KEGG" id="pgr:PGTG_06188"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06188"/>
    <dbReference type="eggNOG" id="KOG0815">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_053173_1_1_1"/>
    <dbReference type="InParanoid" id="E3K826"/>
    <dbReference type="OMA" id="DMNPFKL"/>
    <dbReference type="OrthoDB" id="10259902at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="EnsemblFungi"/>
    </dbReference>
    <dbReference type="CDD" id="cd05795">
        <property type="entry name" value="Ribosomal_P0_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000001">
        <property type="entry name" value="60S acidic ribosomal protein P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050323">
        <property type="entry name" value="Ribosomal_protein_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030670">
        <property type="entry name" value="uL10_eukaryotes"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039087">
        <property type="entry name" value="L10E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="6">
        <location>
            <begin position="109"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="287"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="5">
        <location>
            <begin position="297"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR039087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="EFP80232.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="312" mass="33395" checksum="E4DFE4C3120FEA85" modified="2012-04-18" version="2">MGKPREFKEAYFNKLKDLMAQFPSAFIVNVDNVGSNQMHQIRQALRGKGTVLMGKNTMVRRAIRNILADNPQYERILPLVRGNIGFVFTSGDLKDVRDIITANKVAAPAKAGAFAPLDVYIPAGNTGMEPGKTSFFQALGIPTKIARGTIEIVSDMKIVTAGSRVGASEATLLNLLKISPFTYGMTVVSIYDNGNVFSPTVLDIDEKTLIDQFLSGIKTIAAISLAINYPTVASVPHSLVNSYKNLIAIALTTDYMFEGAQKAKDYLDNPEAFAAAAAPAATASAAAPAAAAAKEPEPEEEEDEDMGLDLFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>E3K862</accession>
    <name>E3K862_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Amino acid permease/ SLC12A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_06392</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178275">
        <property type="protein sequence ID" value="EFP80436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324855.1">
        <property type="nucleotide sequence ID" value="XM_003324807.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K862"/>
    <dbReference type="FunCoup" id="E3K862">
        <property type="interactions" value="3"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K862"/>
    <dbReference type="GeneID" id="10539445"/>
    <dbReference type="KEGG" id="pgr:PGTG_06392"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06392"/>
    <dbReference type="eggNOG" id="ENOG502S504">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_115063_2_0_1"/>
    <dbReference type="InParanoid" id="E3K862"/>
    <dbReference type="OMA" id="LNAWSCV"/>
    <dbReference type="OrthoDB" id="67317at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056552">
        <property type="entry name" value="Ribonucl_Kappa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026770">
        <property type="entry name" value="RNase_K"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31733">
        <property type="entry name" value="RIBONUCLEASE KAPPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23489">
        <property type="entry name" value="V-ATPase_su_f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="EFP80436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="90" mass="9319" checksum="9935B819D6D1531C" modified="2011-01-11" version="1">MVSAAIVGPGLASCCTVLSVIGVVFLVSLGVGFENNAHVLMGSTESPENGKKVARVCFASAIVYACFVGFCSCQLSANRRVVRGGEVHLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3K8E2</accession>
    <name>E3K8E2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Peroxisome assembly protein 12</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Peroxin-12</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">PGTG_06457</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="20" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Protein modification; protein ubiquitination.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Component of the PEX2-PEX10-PEX12 retrotranslocation channel, composed of PEX2, PEX10 and PEX12.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Peroxisome membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the pex2/pex10/pex12 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80501.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324920.2">
        <property type="nucleotide sequence ID" value="XM_003324872.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K8E2"/>
    <dbReference type="FunCoup" id="E3K8E2">
        <property type="interactions" value="227"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K8E2"/>
    <dbReference type="GeneID" id="10539506"/>
    <dbReference type="KEGG" id="pgr:PGTG_06457"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06457"/>
    <dbReference type="HOGENOM" id="CLU_031067_0_0_1"/>
    <dbReference type="InParanoid" id="E3K8E2"/>
    <dbReference type="OrthoDB" id="107372at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990429">
        <property type="term" value="C:peroxisomal importomer complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005778">
        <property type="term" value="C:peroxisomal membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004842">
        <property type="term" value="F:ubiquitin-protein transferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016558">
        <property type="term" value="P:protein import into peroxisome matrix"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016562">
        <property type="term" value="P:protein import into peroxisome matrix, receptor recycling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006513">
        <property type="term" value="P:protein monoubiquitination"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd16451">
        <property type="entry name" value="mRING_PEX12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.40.10:FF:001163">
        <property type="entry name" value="Uncharacterized protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017375">
        <property type="entry name" value="PEX12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006845">
        <property type="entry name" value="Pex_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018957">
        <property type="entry name" value="Znf_C3HC4_RING-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12888:SF0">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12888">
        <property type="entry name" value="PEROXISOME ASSEMBLY PROTEIN 12 PEROXIN-12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04757">
        <property type="entry name" value="Pex2_Pex12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00097">
        <property type="entry name" value="zf-C3HC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038074">
        <property type="entry name" value="Peroxisome_assembly_p12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57850">
        <property type="entry name" value="RING/U-box"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0576">Peroxisome</keyword>
    <keyword evidence="10" id="KW-0653">Protein transport</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <keyword evidence="8" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Pex N-terminal" evidence="18">
        <location>
            <begin position="19"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc finger C3HC4 RING-type" evidence="17">
        <location>
            <begin position="352"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="153"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00018980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00034505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="EFP80501.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="403" mass="45948" checksum="DF4AF26AF3DF9652" modified="2012-04-18" version="2">MDPNEEELYRPTFFELVAQDQLRELIGPVIRYITSVFAQRYPRYLIKLLNHHDEVFAAMISWFITCNSSNPINQSISQNCSGGSFAENFYGLSRRRHRIAKPVSLPDSTVGPQADPGRLAPHDVRLSLIFLVAIPYLRAKAAGLHEALGGGPVNDDLLDDEPERPSPLSNTDDSRMERIKSTTLNCFKSSYPYFIAMTEFSHLIFGLRYLFGKSPYWRASQAYTGIEIRRTSAHDQGRMQHKLDSQKISPFARDLATGHRPNWRLILRRFQSMISHRFFESLKVLLPGSIFFFKFLEWWYSSSNTSRYRPSFSNSNGTQFPAIQPPVPLKPQVKGILGDGTALTKPIPKGHCPICRTKLSNPTAVPSGWVYCYKCIHPYVVEYQQCPVTHFPTNLTNLRKIIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="69" xmlns="https://uniprot.org/uniprot">
    <accession>E3K8I4</accession>
    <name>E3K8I4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Stress-induced-phosphoprotein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">PGTG_06468</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="9" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003324931.1">
        <property type="nucleotide sequence ID" value="XM_003324883.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K8I4"/>
    <dbReference type="FunCoup" id="E3K8I4">
        <property type="interactions" value="564"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3K8I4"/>
    <dbReference type="GeneID" id="10539523"/>
    <dbReference type="KEGG" id="pgr:PGTG_06468"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06468"/>
    <dbReference type="eggNOG" id="KOG0548">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_000134_46_1_1"/>
    <dbReference type="InParanoid" id="E3K8I4"/>
    <dbReference type="OMA" id="WERREFI"/>
    <dbReference type="OrthoDB" id="2423701at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000010">
        <property type="entry name" value="Stress-induced phosphoprotein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.260.100:FF:000002">
        <property type="entry name" value="Stress-induced-phosphoprotein 1 (Hsp70/Hsp90-organizing)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.25.40.10:FF:000027">
        <property type="entry name" value="stress-induced-phosphoprotein 1 isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041243">
        <property type="entry name" value="STI1/HOP_DP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006636">
        <property type="entry name" value="STI1_HS-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904:SF523">
        <property type="entry name" value="STRESS-INDUCED-PHOSPHOPROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22904">
        <property type="entry name" value="TPR REPEAT CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17830">
        <property type="entry name" value="STI1-HOP_DP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13414">
        <property type="entry name" value="TPR_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00727">
        <property type="entry name" value="STI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 5" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="15"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="89"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="183"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="5">
        <location>
            <begin position="217"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="STI1" evidence="7">
        <location>
            <begin position="286"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="6">
        <location>
            <begin position="9"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="EFP80512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="337" mass="38239" checksum="0BAB5185A5DA3497" modified="2011-01-11" version="1">MSSLQDVDPELKAQADNHKNEGNNLYKSRDFAGAIAAYEKAFSLNPDPVYLNNLAAAYFEQGEFDECVKTCERAVEEGREKRVDYKVIAKSFARMGSAYLKKGEYNLAIKNLERSLTEHRTPDTLAKLKEAEKTKAEEERKAYLNPELSDKAREEGNVCFKNGDFAGAVKHYTESIKRNPADPRAYTNRAASYSKLLALPEALKDTDSAIEADPDYTKAYIRKSLTLFAMKDYKKAIDTLRKAEEHDPEKKHSKEIAANLQKCVMAEYGERANETDEQRLEKAMRDPEIQQIMSDPVMQQILSQGQQDPQALQSHMANPMIRQKIMKLVEAGIIKTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>E3K955</accession>
    <name>E3K955_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_06783</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178276">
        <property type="protein sequence ID" value="EFP80827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325246.1">
        <property type="nucleotide sequence ID" value="XM_003325198.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K955"/>
    <dbReference type="GeneID" id="10539849"/>
    <dbReference type="KEGG" id="pgr:PGTG_06783"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_06783"/>
    <dbReference type="HOGENOM" id="CLU_053900_1_0_1"/>
    <dbReference type="InParanoid" id="E3K955"/>
    <dbReference type="OrthoDB" id="2509015at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="48"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="102"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="59"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="104"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="2">
        <location>
            <begin position="117"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP80827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="420" mass="46955" checksum="C7971CE98415291D" modified="2011-01-11" version="1">MSIADEMVVLRKQLAQLQSKLKALESRLEKEVSARIATIKDNSIINDVHSTSPPYPLVKSSSIQKSTRSAQSRDQRTLRSRSFKVMRHLINIVDVSRAASTASELEKKRTTIDSSHDPITSVSSVTELKSSAKPTEATNSPPTKLKASAKTTLGLSLKEKAVRSSNRNDFQASTILLPPITEPGAPVNPKSAKTPPIPPNNLVTALNTSSETPRLFHPKRTRKADLASSNKEANERNLQAVSELPVTKELNTNAHSKLEGAVPKTSETLAITRTSGNKKAEIIEHNDLRRGCSRFALLKYDPAVLDLLPTSVKEKQFIRTSTVTDIENLQQASEILVRRQVLGIWEWREGTSRAALHEYKKPELGTTMSNRRFKFVGFAWKGALGQQPPRIEELHEDISQGADTLRWYWFKDLWLLFYFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="80" xmlns="https://uniprot.org/uniprot">
    <accession>E3K9A4</accession>
    <name>E3K9A4_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Histone acetyltransferase</fullName>
            <ecNumber evidence="3 12">2.3.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">PGTG_07306</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="16" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>L-lysyl-[protein] + acetyl-CoA = N(6)-acetyl-L-lysyl-[protein] + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:45948"/>
            <dbReference type="Rhea" id="RHEA-COMP:9752"/>
            <dbReference type="Rhea" id="RHEA-COMP:10731"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:61930"/>
            <dbReference type="EC" id="2.3.1.48"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the MYST (SAS/MOZ) family.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.48" evidence="3 12"/>
    <dbReference type="EMBL" id="DS178277">
        <property type="protein sequence ID" value="EFP81054.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325473.2">
        <property type="nucleotide sequence ID" value="XM_003325425.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3K9A4"/>
    <dbReference type="STRING" id="418459.E3K9A4"/>
    <dbReference type="GeneID" id="10536374"/>
    <dbReference type="KEGG" id="pgr:PGTG_07306"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07306"/>
    <dbReference type="eggNOG" id="KOG1244">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="eggNOG" id="KOG2747">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_303293_0_0_1"/>
    <dbReference type="InParanoid" id="E3K9A4"/>
    <dbReference type="OrthoDB" id="787137at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000785">
        <property type="term" value="C:chromatin"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990467">
        <property type="term" value="C:NuA3a histone acetyltransferase complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003682">
        <property type="term" value="F:chromatin binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004402">
        <property type="term" value="F:histone acetyltransferase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003712">
        <property type="term" value="F:transcription coregulator activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.60.60:FF:000001">
        <property type="entry name" value="Histone acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.60">
        <property type="entry name" value="N-acetyl transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.40.10">
        <property type="entry name" value="Zinc/RING finger domain, C3HC4 (zinc finger)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002717">
        <property type="entry name" value="HAT_MYST-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050603">
        <property type="entry name" value="MYST_HAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040706">
        <property type="entry name" value="Zf-MYST"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011011">
        <property type="entry name" value="Znf_FYVE_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001965">
        <property type="entry name" value="Znf_PHD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019787">
        <property type="entry name" value="Znf_PHD-finger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013083">
        <property type="entry name" value="Znf_RING/FYVE/PHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10615:SF161">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE KAT7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01853">
        <property type="entry name" value="MOZ_SAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00628">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17772">
        <property type="entry name" value="zf-MYST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00249">
        <property type="entry name" value="PHD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57903">
        <property type="entry name" value="FYVE/PHD zinc finger"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51726">
        <property type="entry name" value="MYST_HAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0007">Acetylation</keyword>
    <keyword evidence="8" id="KW-0156">Chromatin regulator</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 12" id="KW-0539">Nucleus</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="MYST-type HAT" evidence="14">
        <location>
            <begin position="685"/>
            <end position="957"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="185"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="253"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="413"/>
            <end position="617"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="654"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="13">
        <location>
            <begin position="954"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="18"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="41"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="185"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="254"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="413"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="451"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="485"/>
            <end position="495"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="13">
        <location>
            <begin position="521"/>
            <end position="535"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="596"/>
            <end position="613"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="13">
        <location>
            <begin position="661"/>
            <end position="674"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="13">
        <location>
            <begin position="963"/>
            <end position="978"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="13">
        <location>
            <begin position="979"/>
            <end position="992"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="13">
        <location>
            <begin position="994"/>
            <end position="1016"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="11">
        <location>
            <position position="882"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022990"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR602717-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU361211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="EFP81054.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="1108" mass="123939" checksum="D32E63B862D723F3" modified="2012-04-18" version="2">MATMEPINMTIEENLSTDNDDTNDMDAEGELVDSEDEQQELIEQQQQQQYHSTSSSQPSSPSFNTALTEQEQEPIQDIINQKPSSQSTVYEEEEAGQHHHSILIEPIHHPLQDSSPKMAPVRLNKKTTTKRSISTSNKTQLKLIKSQPVNHRSNSKQKRCNSKLLPIVMAEQQILEQHQQQQQKACKQVYNQPNRQQPQQQPTSPVTRPKLGVIGGRRAPREGWCSFCSREGGFDDLNGNMVRGEIPISLVTQSLNPSNDPSHNNSNNLSLEPLSIIPSSSTSSTSRRRGKGEMVSCWECGQSGHFSCMELNNLTIKSHAKSYPWLCLECRRCHGCDKKGDDDQNMLLCAVCDRGWHGECLNPPLRTVPSGDFTCPFDHQSTQCIPPLPDSVTLPPFSPSQIPLGLPTIPNNTPLKANGITSKPNNRATVNKKRKMKNLPSDDDDESMGFVDKETGDPTEMRSSKKLTAGKPRNGTKGTVKRLNTLTSTTSSSLSPLRKQTSSLTLDGTALDPFSNHPDGSDENIDDAQEEEEQGGDSLDPPKPTNPFEGVLNEDEAAIGDRNITEDDLTRFNQSLERSKARMETSAGESADRTNAAAPSPSMVAVPSPSSGSLETPRLAAVRERRSGLHGTSPSGTRGRITPTVFTPEMEIDEVSSNSSTHHEEVVKKKEAARPSRRTKKNETHQTSIIKSIRFGEYEIDVWYQAPYPEEYSKLPGGRLWICERCLKYFKTEFEISRHRMKCKNFYPPGDEIYRDNDERHGVRIQIFEVDGRKNKMYCQNLCLLSKMFLDHKTLYYDVDPFLFYVITQTSLLSNHPSSIDSSPSNPRCQFVGYFSKEKRSPTNNVSCIMTLPVLQRKGWGNLLIDFSYLLSKKEKRVGTPEKPLSDLGLLSYRNYWTLSISKFLMSCDPSNEEEQITLEDIANQTSISLLDVYYTCRHKNWIHEVKPSPLSNTSVPVVHHPVVPKRKSHNWNGRRKPPSSVHASPAPVHASQARPNQPGGSSNTNPSAPSSKTNSSLIIDEKAIPKYYNIRWDPLEVSQIVEKSAQKDLLCLKPEKLVWSPFLTTRAQGLSIDVSPIAIPLIPNTDPYQFSNKKLASSSVDQSPLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3KAK0</accession>
    <name>E3KAK0_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_07038</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178278">
        <property type="protein sequence ID" value="EFP81417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003325836.1">
        <property type="nucleotide sequence ID" value="XM_003325788.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KAK0"/>
    <dbReference type="GeneID" id="10537071"/>
    <dbReference type="KEGG" id="pgr:PGTG_07038"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07038"/>
    <dbReference type="eggNOG" id="KOG4476">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_336827_0_0_1"/>
    <dbReference type="InParanoid" id="E3KAK0"/>
    <dbReference type="OMA" id="NGRIWIQ"/>
    <dbReference type="OrthoDB" id="5572844at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018608">
        <property type="entry name" value="Gti1/Pac2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28027">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR MIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28027:SF2">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR MIT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09729">
        <property type="entry name" value="Gti1_Pac2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="172"/>
            <end position="307"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="396"/>
            <end position="569"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="599"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="795"/>
            <end position="860"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="44"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="172"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="208"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="228"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="272"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="283"/>
            <end position="302"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="398"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="447"/>
            <end position="491"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="492"/>
            <end position="502"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="516"/>
            <end position="526"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="552"/>
            <end position="567"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="608"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="635"/>
            <end position="648"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="665"/>
            <end position="679"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="689"/>
            <end position="703"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="711"/>
            <end position="723"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="765"/>
            <end position="781"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="795"/>
            <end position="807"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="838"/>
            <end position="854"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP81417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="860" mass="90570" checksum="B1A427BA423AC4AB" modified="2011-01-11" version="1">MNPNLNNPSGIHHHHQQTSHSVSQLQPSIHHLHSHTHLPPAHTHPSQAHLPHQSQQHHHPSDPTPIRDQIDRQQQNRSNIEQYKSSAIPAYEGYIGSTHDALIIFSGCYLGEFPMVSRRLHERERRSIRSGSVYVFDETKAGIKRWTDGRVWSPSRILNNFLVYREIDQKRPTATTNASGSGTNPPNSNAGAPSATSTNSNHKHGKDNGSQPKSGTSNQPASAGPASHNPTGPSQHSTSNSQYSCHHSTNPSGQSTSQAESRNSNELASLDVKPRNSKEENQEQSQATPGENQTHSAVTQRPMNRERERSLVGSLTSTYKFRPDGLVKKTISIAGMHMISYYKLDDVVSGRLRAPTSHASLLRMQIVGHLLNPGFFRAPPNWGISNGRFWIQEEGPEDSSQMMNSVTSSAPSIHSAHRPGALALPASGSTSSNPPQGIRPSHPPARPSASERISSSALSRPGSSASSTGSPNATSPTSSHSFLLLSTSNLSNPGATPSSPVINTVGGRPNFPRVHNSTNTSTASGSGHLGRPSIMSGASANRYEPYPPAARSPPLSASSTTNTSTLADQQTSVQYFSSNHTSVGLNGYSTHSFLSDSHSAPLVGSDRPSANNRNAHNDSSAAHDGRSLESVGQVSGYDDSGGYRSGTSTSGGGESLYSQYAVTDPANNSSRSASVPPSSYGLPSINRLGASGQPASSSASTGARGIGDYQFTGSPSQPQTNRLSPSTGSHGGSYGGYLAPPMPENTFRTTSGGSGLAERENNTYGSSSHSINSITNTSGQASSAAGAQSASFLYPSTSTSPSSGYSFNNPARRGSAMATLSGDINNGSADSTRDGARINSQNRTNGSSLQMNENGGNGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>E3KCM3</accession>
    <name>E3KCM3_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_07572</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178281">
        <property type="protein sequence ID" value="EFP82175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326594.1">
        <property type="nucleotide sequence ID" value="XM_003326546.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KCM3"/>
    <dbReference type="GeneID" id="10529547"/>
    <dbReference type="KEGG" id="pgr:PGTG_07572"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_07572"/>
    <dbReference type="HOGENOM" id="CLU_1540845_0_0_1"/>
    <dbReference type="InParanoid" id="E3KCM3"/>
    <dbReference type="OrthoDB" id="2508303at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP82175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="174" mass="19310" checksum="8EC6A7038C60CFAE" modified="2011-01-11" version="1">MKIGCPEPSVPEFKSCGQQLQLASPHQLSSTMLGLPIIVKLTLVCTLLSKCLGVLAPPPVHSPDDLLLTGQCWECFERSGHLWEGPFPNSMILTWEDDEYPCRKLSLDERCNQKVKLKQYYCSLCGRYAHFNLGGCSVHRPVTRYLAGDPPELEVPLPGDPPESQVKILPALLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="74" xmlns="https://uniprot.org/uniprot">
    <accession>E3KE12</accession>
    <name>E3KE12_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AP-2 complex subunit sigma</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Adaptin small chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Clathrin assembly protein 2 sigma small chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Sigma2-adaptin</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">PGTG_08325</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="19" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Component of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit APL3 and beta-type subunit APL1), a medium chain (mu-type subunit APM4) and a small adaptin (sigma-type subunit APS2).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="2">Membrane</location>
            <location evidence="2">Coated pit</location>
            <topology evidence="2">Peripheral membrane protein</topology>
            <orientation evidence="2">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the adaptor complexes small subunit family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326788.1">
        <property type="nucleotide sequence ID" value="XM_003326740.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KE12"/>
    <dbReference type="FunCoup" id="E3KE12">
        <property type="interactions" value="361"/>
    </dbReference>
    <dbReference type="STRING" id="418459.E3KE12"/>
    <dbReference type="GeneID" id="10529746"/>
    <dbReference type="KEGG" id="pgr:PGTG_08325"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08325"/>
    <dbReference type="eggNOG" id="KOG0935">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_061221_3_0_1"/>
    <dbReference type="InParanoid" id="E3KE12"/>
    <dbReference type="OMA" id="QSNFVEY"/>
    <dbReference type="OrthoDB" id="371463at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030122">
        <property type="term" value="C:AP-2 adaptor complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035615">
        <property type="term" value="F:clathrin adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072583">
        <property type="term" value="P:clathrin-dependent endocytosis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016192">
        <property type="term" value="P:vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd14833">
        <property type="entry name" value="AP2_sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.60:FF:000011">
        <property type="entry name" value="AP complex subunit sigma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016635">
        <property type="entry name" value="AP_complex_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022775">
        <property type="entry name" value="AP_mu_sigma_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027156">
        <property type="entry name" value="APS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011012">
        <property type="entry name" value="Longin-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11753">
        <property type="entry name" value="ADAPTOR COMPLEXES SMALL SUBUNIT FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01217">
        <property type="entry name" value="Clat_adaptor_s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64356">
        <property type="entry name" value="SNARE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10" id="KW-0168">Coated pit</keyword>
    <keyword evidence="7" id="KW-0254">Endocytosis</keyword>
    <keyword evidence="9" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="domain" description="AP complex mu/sigma subunit" evidence="17">
        <location>
            <begin position="57"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="16">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00025487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00030104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00031686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00062168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="EFP82369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="199" mass="23331" checksum="025470FF72CC092F" modified="2011-01-11" version="1">MEVGRSWRCPTTPADRPSEKEAHTLNIDHPPIPPRTNIIQTQPEAPDSWPTHPEAEMIRFILVQNRQGKTRLSKWYVPLEDEEKVKVRGEVHRLVATRDQKYQSNFVEFRTSKVVYRRYAGLFFCICIDSNDNELSYLEAIHLFVEVLDAFFGNVCELDLVFQFHRVYSILDEVFLAGEIEETSKSVILERLDYLEKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>E3KE19</accession>
    <name>E3KE19_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08332</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178282">
        <property type="protein sequence ID" value="EFP82376.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003326795.2">
        <property type="nucleotide sequence ID" value="XM_003326747.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KE19"/>
    <dbReference type="GeneID" id="10529753"/>
    <dbReference type="KEGG" id="pgr:PGTG_08332"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08332"/>
    <dbReference type="HOGENOM" id="CLU_016504_0_0_1"/>
    <dbReference type="InParanoid" id="E3KE19"/>
    <dbReference type="OrthoDB" id="2496723at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="14"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="51"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="64"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP82376.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="673" mass="78125" checksum="D5472C9872F41AE4" modified="2012-04-18" version="2">MADDFSGWIFPNEISEKSSLSDRASRIEQAPPSPELIQSVTQELRVENQAPVTPVPTNTNSPPEASTSSPSLGRNTLNEIEPYVSEKDDEHFDLFDAKYGEIKRAKEPTDDLARKITTSAYYDRSDKMWLWIFVNTVYRAEPHEQLYRSTYGSTIAKFNDLFAADVGKFLNQGRDGIALDKIRSREFYEIILQFSDIFWVNNLRLLGSLGVSEEAELLKQHASIYRWLLCCNEHDLIPALEPFKLVITMKQSDEKVYRIMKRKNKFTVDIGSVSERQGFLTEAAIILLATYYKTVNLEKWEVVFENDFNFVSHMSKTATESFYSATRIARREMHHLQLFPWENACDLSSTYAVLRKVQFTKPCGVDFKRYINEIDLPRSEVKEEDEEIGAIPLDLPETGKIDCEQDMKTGIWARWGHISMIATNLQSRRLEEPLRVDSTGIMVEIKKFKMRQGKKKLHFAHKTISERGFVGRAGRLLEVLLALNQRLMETLGHPLTNSIDLEEQNKVQLFFLQLITSSPASLSIDPKINEQIDLGKIAFIQVSLFKAIECRSTEKVYKLYDRAPRLSRQNLIMCEVAVKTLAYYYHRESYIKWINVFEDEAKFFKSFEKIAKRIKTNSDYSRFSRDSFPEMRKAKMIPWRNPVVITNRQKVTITRLFKIDHYAQLKSKKKDKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KGQ4</accession>
    <name>E3KGQ4_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_08665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178286">
        <property type="protein sequence ID" value="EFP83479.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003327898.2">
        <property type="nucleotide sequence ID" value="XM_003327850.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KGQ4"/>
    <dbReference type="GeneID" id="10532156"/>
    <dbReference type="KEGG" id="pgr:PGTG_08665"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_08665"/>
    <dbReference type="HOGENOM" id="CLU_994460_0_0_1"/>
    <dbReference type="InParanoid" id="E3KGQ4"/>
    <dbReference type="OrthoDB" id="2505725at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="111"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="260"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="111"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="182"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83479.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="280" mass="30949" checksum="06952D5C3E891860" modified="2012-04-18" version="2">MVILHILCKFTLQLQSQTPPALDIARKLVRPGRLLRIIHQDVEVFSTNRHRRQGTCPWSIGFHRSQAGNLWPEGCRRSLDWAPELTGNFGRYTSSNWGQLGPIIYIRTSPRLSSSPGNTSNAQACPSRLPNSLPSTSPRSATMSPKGTAKNCSLDCASSPLNPVKHTPPSSTRLWSAASRPTISHSSNASSSTTSSVLPSLANFYAIHLHPHLIPFAKSMYQTLFIDDNPLKNQLKTRLLRPVKALHLIYVKPQIKAIPPPVDDHHHPASAYGRLQRSAD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>E3KH34</accession>
    <name>E3KH34_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DDE Tnp4 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">PGTG_09322</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="10" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Nucleus</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HARBI1 family.</text>
    </comment>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83609.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328028.2">
        <property type="nucleotide sequence ID" value="XM_003327980.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KH34"/>
    <dbReference type="GeneID" id="10532293"/>
    <dbReference type="KEGG" id="pgr:PGTG_09322"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09322"/>
    <dbReference type="HOGENOM" id="CLU_018552_2_0_1"/>
    <dbReference type="InParanoid" id="E3KH34"/>
    <dbReference type="OrthoDB" id="3233403at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045249">
        <property type="entry name" value="HARBI1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027806">
        <property type="entry name" value="HARBI1_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22930:SF85">
        <property type="entry name" value="GH03217P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13359">
        <property type="entry name" value="DDE_Tnp_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-0539">Nucleus</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDE Tnp4" evidence="8">
        <location>
            <begin position="220"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="EFP83609.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="432" mass="49983" checksum="F3C8C6A08621166A" modified="2012-04-18" version="2">MRGYSERQSLIRDLFLIIVWLEHDELEDMVDSLFHHIRMPTEAEIMFPRSKFQQRIYDLLLSDRAMFDEALRLVLSNRYLVPRPTPKLRDEFDLERLFNMPDIEFRQSSRTSKAGFVGLLNIICMNPVFHRGGIRPQLPIAHQLALTLERLGSNGNGASVGRFSRNLSVGRGTVVKVSRRVIEAFISLGRRYVVWPDSARRAEISEVMSREGFRGCVGFVDGTTIPMFQRPGYDGEVFFDRKRCYSINAQIICDCDKYITSFITGWPGSCGDSRVYKRMQLHLNPSNYFEEGQYLLADSAYELSHTVIPAYKVPAANITINSQFNYCLAKARVRNEHTIGVLKSRWSSLREMRLHLYCRQHMRAYVAWLYSCIILHNILAGLGDQWAELDDHESLPPDDDSDEDDIDESAEDFRDRLTINCVCYNMYQRGIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KH57</accession>
    <name>E3KH57_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">U1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_09345</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178287">
        <property type="protein sequence ID" value="EFP83632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328051.1">
        <property type="nucleotide sequence ID" value="XM_003328003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KH57"/>
    <dbReference type="GeneID" id="10532318"/>
    <dbReference type="KEGG" id="pgr:PGTG_09345"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09345"/>
    <dbReference type="HOGENOM" id="CLU_004591_4_2_1"/>
    <dbReference type="InParanoid" id="E3KH57"/>
    <dbReference type="OrthoDB" id="10281413at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP83632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="174" mass="19974" checksum="9CD1C1EFF7312269" modified="2011-01-11" version="1">MPPDKYFFQEGSNQFSCILCRGKSTSNQKKHARGKRHLNLVRLDEERIERQKERIAANETLNQHESNQPQIEIEPICDDPVCSNEQVEPLVNETDDESDVVPISLFEDGESEHEEDELDWLNLLGVAIDQIDDEPADSFDGLLVPAFQREVAEIEDIMTVDSVWFPFVNKDVCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>E3KHV0</accession>
    <name>E3KHV0_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MICOS complex subunit mic19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_09588</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178288">
        <property type="protein sequence ID" value="EFP83875.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328294.2">
        <property type="nucleotide sequence ID" value="XM_003328246.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KHV0"/>
    <dbReference type="STRING" id="418459.E3KHV0"/>
    <dbReference type="GeneID" id="10532564"/>
    <dbReference type="KEGG" id="pgr:PGTG_09588"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_09588"/>
    <dbReference type="eggNOG" id="ENOG502S4WP">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_093897_1_0_1"/>
    <dbReference type="InParanoid" id="E3KHV0"/>
    <dbReference type="OrthoDB" id="5544375at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012471">
        <property type="entry name" value="DUF1690"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07956">
        <property type="entry name" value="DUF1690"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="80"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="83"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="117"/>
            <end position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP83875.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="182" mass="20768" checksum="F3DB085FE5900FAB" modified="2012-04-18" version="2">MANSSNAEHSSEKGKGVEMTIEGDEQVYHNPKSQVRLQQPLIDRLFEVSDDHPSASRQAILDSSIQQKLKADRERLNQLGSEVDSKVREAFRKQSSSQQTKDDDKSNSSAQLMEEISSIKERVQQRRNENTSEFLPEITHARSAIVACLKQYQDEPLKCTEQVNNFKKVVRNLESKFVTSMQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KII8</accession>
    <name>E3KII8_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_10491</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328532.1">
        <property type="nucleotide sequence ID" value="XM_003328484.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KII8"/>
    <dbReference type="GeneID" id="10545211"/>
    <dbReference type="KEGG" id="pgr:PGTG_10491"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10491"/>
    <dbReference type="HOGENOM" id="CLU_004591_4_1_1"/>
    <dbReference type="InParanoid" id="E3KII8"/>
    <dbReference type="OrthoDB" id="2507436at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912">
        <property type="entry name" value="IP13529P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31912:SF34">
        <property type="entry name" value="NOTOCHORD-RELATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP84113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="214" mass="24995" checksum="22BF079C8F2058AB" modified="2011-01-11" version="1">MLVDEEINEEVDKIVPGTFEVAESEDDEEAEKSWMDFVGVAIGQIDSELAVDDPANDSPLFRREEEKIRKVTPEDSEWFPFLNKEYLIGSLLVRYLHKLISRDLYHQIRVIFMLNQIKLPRWEALRLMRAQIRTLVNQTIVEQETVFGQPVFGLKASNVIKHDLMNPLVAPHMDFFPEDAHGKNIYKLSQSTKWLKHLASDLRVQMIKQGKFYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIM2</accession>
    <name>E3KIM2_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_10525</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84147.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328566.2">
        <property type="nucleotide sequence ID" value="XM_003328518.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIM2"/>
    <dbReference type="GeneID" id="10545325"/>
    <dbReference type="KEGG" id="pgr:PGTG_10525"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10525"/>
    <dbReference type="HOGENOM" id="CLU_769725_0_0_1"/>
    <dbReference type="InParanoid" id="E3KIM2"/>
    <dbReference type="OrthoDB" id="2496479at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="169"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="69"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="69"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP84147.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="360" mass="39932" checksum="FAB32A9CA58451BF" modified="2012-04-18" version="2">MLMIQCSQLHYILIFVLFSGKIRKSAQCRTGLQADRLSGEFKGKSAVQDEAQNPSPRLKSYYHQIQTNQESQNVNEYSSPVSKAEEASSVEVPPNTPPSSKNDTFEQPPKDPSEIIPSSTSLISPKVATPHHDVKPKVAEISPTPSSESLSKPHVGYGKQQSMIITRDLLIAISVLALSMLSLGIIWFALIVFQPMIRSSRMRLKPRSWFKKNTKPNDPPQWWTQFPINSQLSEAEALSITRNFTGGANAGRSQFGSTSEMVAAFTPRKVSWNMHTGLTEQVEVLEDPESQWKPDNAPSGENPIPHSRLETWLRGTDEMRGKLFLATSVFQKDPSLCLTKESALMRGLPSLGPSDSASRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>E3KIV9</accession>
    <name>E3KIV9_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">RAD50-interacting protein 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_10612</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84234.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328653.2">
        <property type="nucleotide sequence ID" value="XM_003328605.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KIV9"/>
    <dbReference type="STRING" id="418459.E3KIV9"/>
    <dbReference type="GeneID" id="10545495"/>
    <dbReference type="KEGG" id="pgr:PGTG_10612"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10612"/>
    <dbReference type="eggNOG" id="KOG2218">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_350593_0_0_1"/>
    <dbReference type="InParanoid" id="E3KIV9"/>
    <dbReference type="OrthoDB" id="407410at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070939">
        <property type="term" value="C:Dsl1/NZR complex"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006888">
        <property type="term" value="P:endoplasmic reticulum to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060628">
        <property type="term" value="P:regulation of ER to Golgi vesicle-mediated transport"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006890">
        <property type="term" value="P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.670:FF:000019">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.670">
        <property type="entry name" value="Dsl1p vesicle tethering complex, Tip20p subunit, domain D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042044">
        <property type="entry name" value="EXOC6PINT-1/Sec15/Tip20_C_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007528">
        <property type="entry name" value="RINT1_Tip20"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13520:SF0">
        <property type="entry name" value="RAD50-INTERACTING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13520">
        <property type="entry name" value="RAD50-INTERACTING PROTEIN 1 RINT-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04437">
        <property type="entry name" value="RINT1_TIP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51386">
        <property type="entry name" value="RINT1_TIP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP84234.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="922" mass="106037" checksum="DD5DAB8C9CE9E965" modified="2012-04-18" version="2">MMTMRMTTMRSMGIKEEDSTKEDGIDKLVELLNLPYSHNDAHQLRQLSIQAHHQISLLSNDTTTTTTTLDGLSDQEILDQLIQKTGIAETQFQAIDDQLSESTKEVQKMMGKAQEEIAKLKPMSERIHEEFMKYEDEYIDHYESLKPLPTSLENAPQRAQEARRQPKNSSVLIESMSEQIEWLKELGRLKLWFSVLLDFRDHTDQLVQGATQTDPLSSTNSSSSSSSIREIEALADRLFELIQSYQDFSRLTPPNHFSQFNLYQYIYQIIRQSLNSLIEIISGHVAQTLENEIEWPQPSRAPLDFKSPSTKRILDSFKTAVNFQNKLHSRPDLVSLVERRPDPVDQSSVIWLPLLEGSILLKAILRPIILRFRFHFDGQRATNRLDKPEWYFNHVLDRLAEHEKFIKSDIQKLFELSGQGSTKVFNGFTVQLIKIVEKKLKRSIPGILEMKPILAHTIEKAIGFDQMIRQMHYLESDSSKTGVPSTTQWLGTVDGILSNQHWFNIWFEAEKRFVDDMCLEIISSKETWEINDEDQRPISMNLPATNSALRIQDLVEQIKSKYEGLPRLSYQAEFLVKIQVVVLEAYSQRISGVLDGFERNRLMNVVGGDSGRAKMNLGLKGLERLAKVYVSCNWILSCFRSWNDDLFYAELYEKLKQEKTPPTPEVERFLRSVEAVGALDPTLFSPSVVRFEELAKRTEAAIVRQVVQEIMTELKPYFAKRWDIEEEEGAEGSPGEVDELSREIVPAISLWKSRMNYLRQTVQPTVKLARLVKPIAKEIEAKLLLKIIFEPFSIRAITRRGGLQFHFDLLFGWLNFSTSGTTTHFGSGSSDHQRHSSLRLEKHFARVLDACTLLAHPLRPSSSEGPSSSSQFSFEELVRLCFDELLDESQLKSLLKERLQISSLSRSECQAVLKRRPECWKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KJ15</accession>
    <name>E3KJ15_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_10668</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178289">
        <property type="protein sequence ID" value="EFP84290.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003328709.2">
        <property type="nucleotide sequence ID" value="XM_003328661.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KJ15"/>
    <dbReference type="GeneID" id="10545634"/>
    <dbReference type="KEGG" id="pgr:PGTG_10668"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10668"/>
    <dbReference type="HOGENOM" id="CLU_131659_0_0_1"/>
    <dbReference type="InParanoid" id="E3KJ15"/>
    <dbReference type="OrthoDB" id="2500184at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <feature type="signal peptide" evidence="1">
        <location>
            <begin position="1"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173434" evidence="1">
        <location>
            <begin position="27"/>
            <end position="174"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP84290.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="174" mass="18507" checksum="B8A1FB5E8FAF0850" modified="2012-04-18" version="2">MFSQQPSHLFLYYLLALASDIAPLSASSSSTPRTCTNVYQPISTSGDQTANQMTCRGTGNPQRSFICDQKSCEGSTTCQTCVAQLTNVSVNSIECAKYYVDDNRTNCWTSADEQFTCYGKCTGAAVCSDCNLDEQTPSISNCSGDSPSKHGTKASHASAASASNAGVTTWLAWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>E3KK16</accession>
    <name>E3KK16_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">K Homology domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_10800</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178291">
        <property type="protein sequence ID" value="EFP84641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329060.1">
        <property type="nucleotide sequence ID" value="XM_003329012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KK16"/>
    <dbReference type="STRING" id="418459.E3KK16"/>
    <dbReference type="GeneID" id="10546310"/>
    <dbReference type="KEGG" id="pgr:PGTG_10800"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_10800"/>
    <dbReference type="eggNOG" id="KOG2190">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_022670_4_0_1"/>
    <dbReference type="InParanoid" id="E3KK16"/>
    <dbReference type="OMA" id="GECETIQ"/>
    <dbReference type="OrthoDB" id="442947at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005634">
        <property type="term" value="C:nucleus"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051252">
        <property type="term" value="P:regulation of RNA metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10288">
        <property type="entry name" value="KH DOMAIN CONTAINING RNA BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="104"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="4">
        <location>
            <begin position="187"/>
            <end position="258"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="20"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="267"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="335"/>
            <end position="378"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="20"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="35"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="64"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="82"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Gly residues" evidence="3">
        <location>
            <begin position="272"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="341"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="357"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="369"/>
            <end position="378"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP84641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="378" mass="38048" checksum="A9EF243B00F77F20" modified="2011-01-11" version="1">MSSIPSIVIDEPNLNSTAVAPLATGTNAVPNGIDNPNGSAPSNNGPQPNNESAEIPSHLPSVSHDPNNVRPDSQPQPSMGLQHQGTSSQQQQQQNAAHAVAATQTIQMRALIVTQDASIIIGKGGRHINEVREKSGARATISEAVPGNAERILSVAGPLDAVSKAFGLIVRRINDEPFDVPSVPGSRAVTIRFIVPNSRMGSVIGKAGSKIKEIQDMSGARVQASEALLPGSTERVLSISGVADAVHIAVYYVGMILVDNQERMPANTSYRPGGGGSGGGGGGGPGHLGHSGHGSQLGHHHMGGRAPPGALGPVGGGGGGGGRGGGGYGPYAGGASAMPHGGHHHNAHHHGGPPSAGPAAGYPNPAHQPGSQTQQILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLF6</accession>
    <name>E3KLF6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_11300</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP85131.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329550.2">
        <property type="nucleotide sequence ID" value="XM_003329502.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLF6"/>
    <dbReference type="GeneID" id="10527112"/>
    <dbReference type="KEGG" id="pgr:PGTG_11300"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11300"/>
    <dbReference type="HOGENOM" id="CLU_043422_0_0_1"/>
    <dbReference type="InParanoid" id="E3KLF6"/>
    <dbReference type="OrthoDB" id="2500693at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="40"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="213"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="471"/>
            <end position="509"/>
        </location>
    </feature>
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        <location>
            <begin position="70"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="471"/>
            <end position="483"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Acidic residues" evidence="1">
        <location>
            <begin position="499"/>
            <end position="509"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP85131.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="509" mass="56590" checksum="96214565A3CEDF0A" modified="2012-04-18" version="2">MLLNFDKFEDPDDLLCTQQKLLGSRLVLPEATNTPVVMASSATLEPSPTSAPPLDGVGNKAPSKSRKRRQVEPEDDHNPRYPQPLEELMKMTAVELRKIAQENSKEAFSVDDEKFFLDFYHQQEVMLAIKAIERQVSLPMIHTLLGRRVAIREANRWNRFLQTPEARAIFQANGRGVKNKQVMALLSVAYGKLTEAEKEALTAPPVAATNQVSDITIEQAVPTGDSQPRRGDEDLTPSELALDDESEDDSGHRALGSGGGQAPQAFLRGTVSNLVRKDQAEKAINKWSAESLNIAKTCNCEVVIFAVSKHLSSHSFKFERCTPGAIKEHTTILEMDGDNHYSARLQALLTGNSVGQITVANKLPGNQKKKLRNLLPQLTAQLAVHINRETHGVLTSWPWTDNNKRLAEAKYRLELSHLAVSDINWLRRSNKKLKKTHVSMLLLDLDRGYIKVVPVLPTPTASNTELRHNIQGSAQTDQTSSSRSPPPDNTRLNLNQTDSDSENDDQDEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLK0</accession>
    <name>E3KLK0_PUCGT</name>
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        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_11344</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
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        <property type="protein sequence ID" value="EFP85175.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="XM_003329546.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLK0"/>
    <dbReference type="GeneID" id="10527158"/>
    <dbReference type="KEGG" id="pgr:PGTG_11344"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11344"/>
    <dbReference type="HOGENOM" id="CLU_2039209_0_0_1"/>
    <dbReference type="InParanoid" id="E3KLK0"/>
    <dbReference type="OrthoDB" id="10288343at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP85175.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="121" mass="13532" checksum="BFF8AF78158349A1" modified="2012-04-18" version="2">MFTYQIDVMEPTPNEQTHSAAPSLPAPYFQFLLDLVKSTPGILELVKSGPGMTEKWERLEMELEAYYAGPGKPINALKLSIVEAPLPESAMPLLDQLKRVHPDLENPEVELRNRVMLFGGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>E3KLL2</accession>
    <name>E3KLL2_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">GDP/GTP exchange factor Sec2 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_11356</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178293">
        <property type="protein sequence ID" value="EFP85187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003329606.1">
        <property type="nucleotide sequence ID" value="XM_003329558.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KLL2"/>
    <dbReference type="STRING" id="418459.E3KLL2"/>
    <dbReference type="GeneID" id="10527170"/>
    <dbReference type="KEGG" id="pgr:PGTG_11356"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11356"/>
    <dbReference type="eggNOG" id="KOG4324">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_327335_0_0_1"/>
    <dbReference type="InParanoid" id="E3KLL2"/>
    <dbReference type="OMA" id="RNECEAL"/>
    <dbReference type="OrthoDB" id="1748564at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051286">
        <property type="term" value="C:cell tip"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd21044">
        <property type="entry name" value="Rab11BD_RAB3IP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.910">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040351">
        <property type="entry name" value="RAB3IL/RAB3IP/Sec2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009449">
        <property type="entry name" value="Sec2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14430">
        <property type="entry name" value="RABIN3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14430:SF0">
        <property type="entry name" value="SEC2P DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06428">
        <property type="entry name" value="Sec2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144284">
        <property type="entry name" value="Sec2 N-terminal region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GDP/GTP exchange factor Sec2 N-terminal" evidence="4">
        <location>
            <begin position="117"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="1"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="289"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="406"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="553"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="685"/>
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        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="842"/>
            <end position="930"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="102"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="209"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="1"/>
            <end position="19"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="3">
        <location>
            <begin position="31"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="80"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="289"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="304"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="327"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="406"/>
            <end position="422"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="573"/>
            <end position="587"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="685"/>
            <end position="722"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="734"/>
            <end position="744"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="843"/>
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        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="868"/>
            <end position="892"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF06428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP85187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="971" mass="105797" checksum="B14B0A4CEAA96B8F" modified="2011-01-11" version="1">MTTISTRTIRTTSPSTNNNLQPPGCFNNSTNHHHHLNNHHHHFSSPPSQSSLSPSSSSSSSASSSVTITDPSIHSHPNHIPSTLQHIQPEQDSGDSELTDQLNQLNLKLVRSFERISNLEDELHESREKNKKLVARNTLLELESAQHKEALKGGILVERANVQLELQRLTQRVTLESERRSQSEASLSTINQELDDLSVSLFTEANRLVAAEKIQRMRAERKVMEVEEAMKRVEEVIEARRDQTNHLRLSLEAAESQRDQYKAECETLRNQLNSSHSDSFTGLNINPTSSIDSLKKSSPSGSIVNSRESLGTPLHNNPLSIPHNEQARSPQFSPYSSISGASGFHSTPQLKLSQETIPFTEFLQFVHYLRRTREETLTRSSQPPGLTESYYSAAAAITNIGITATPSSRPVSSMSSSSFSSSKEPAPALGPKGSIAPLLPLTQHLSQPFVKRCLEEDSDPALRLDLAPGLNFMSRRATLNALLEGNLVIEPVWSESGQASGFENCTLCGCSLDPWLSARAPSTGSSNHVASSNSSTAGSTVRKMLRGGAWSFGGIGKPKRASHPVTSTQPAEKSTLVPSVSLKLPSSGRSTPNLDDTASLHIHTLKTADGSSTRYPICPTYCLARLRAVCHFWTFVRSLERGVLLDESFWFAHYINQSPASSRFTETHSLGLAYDHALRRIRGQNRSIDSVDGSSPLTHSVQSSAFTTDNNALQIQSSNPTDLSFEISGEPNPEESKSLTKESIDAQVTIPSEEAHQDSSNPTLKPPPTPDPVHLSQLSPSPSPTSTTEHDMMQELAGSDAHHSDQTDTCGTSLTIRPTVLSNEVPILSPAPSPALRRIIGRSPSQTVSGLPSTLSKGHGTGEGPESSLKTLEVTDSNQLGGGESTAQSAHTTGHGDQRHVDGATPLRNPPRSAPMSSFENSCHLRHQSRQKSQSSLELMEAGWEEKCWQQVVKLKEEIFFKRVGLSLVPN</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KMR6</accession>
    <name>E3KMR6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_11947</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178295">
        <property type="protein sequence ID" value="EFP85591.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330010.2">
        <property type="nucleotide sequence ID" value="XM_003329962.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KMR6"/>
    <dbReference type="GeneID" id="10544151"/>
    <dbReference type="KEGG" id="pgr:PGTG_11947"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_11947"/>
    <dbReference type="HOGENOM" id="CLU_1750608_0_0_1"/>
    <dbReference type="InParanoid" id="E3KMR6"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="21"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="87"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="1">
        <location>
            <begin position="95"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP85591.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="149" mass="16908" checksum="0A52338E4C910985" modified="2012-04-18" version="2">MVAELAGVRRSTNRSRNRTPWHWAFLLSLQHVLMYGWMYKIYVKLPETQNRVHPFCPCNNNSTSTLSTLLICLQTAAAAATSQNLDTCKNRHHSPSSSNNPQPTTHTNDTQPLAPTTTPITDPDYPNHSFIYSVCLELILKLQTPERLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>E3KPI5</accession>
    <name>E3KPI5_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_12176</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178299">
        <property type="protein sequence ID" value="EFP86220.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003330639.2">
        <property type="nucleotide sequence ID" value="XM_003330591.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KPI5"/>
    <dbReference type="GeneID" id="10540230"/>
    <dbReference type="KEGG" id="pgr:PGTG_12176"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12176"/>
    <dbReference type="HOGENOM" id="CLU_611299_0_0_1"/>
    <dbReference type="InParanoid" id="E3KPI5"/>
    <dbReference type="OrthoDB" id="10298981at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="115"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="20"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="73"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="115"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP86220.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="448" mass="51246" checksum="4B7506B1C4C33B65" modified="2012-04-18" version="2">MPPPRDPRFRLPSKKKQSQKLKDKAVNSDKAAKKKSTNKTVPDTKKQTTPLSDVRSPLPPPSEHPSDDEMDYDGDKDRSQDDRIIEDENDLDKRPSTIDPVIDELNQQFHQEFGQSQPDTFSSMTRPAGPQPSFHLEDLKNLRRYTKLDDQHFLEAFTILNTPGAAIPFLVMKEFERIQEGSQVQPQAPKDKSDEISTDGFAYSNALKNRVRDITKAALLRPHLDQYTRGKWKPGTSDSSLFTFVMQDLMALPLEFRKTHFPAAMEKDCEVKEQFFKMVREMHRRIRLAIRERLLKNIINSKGDLIEEGPVPLLCDIARAIFRYLHPAEATMTDADVDKAIPVLWYGQIGHLRLQTVDLLVHSQFKKVSQWALIDDKINEVKARGTDYRAAFGKAVLVKDEALFGQGKTFVEILEDDEENIAMPNEDEIQVQFDIIIRNRMASSTGNT</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>E3KQ44</accession>
    <name>E3KQ44_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">GATA-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">PGTG_12375</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
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                <person name="Rouze P."/>
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                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
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            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
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</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
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        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
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        <lineage>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="4" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
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        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4">CRL 75-36-700-3 / race SCCL</strain>
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    <evidence type="ECO:0000256" key="1">
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    </evidence>
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        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="EFP86635.1"/>
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    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
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</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>E3KR71</accession>
    <name>E3KR71_PUCGT</name>
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        <recommendedName>
            <fullName evidence="9">Beta-xylanase</fullName>
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            <taxon>Pucciniaceae</taxon>
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        </lineage>
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    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
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                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="14" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
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                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Endohydrolysis of (1-&gt;4)-beta-D-xylosidic linkages in xylans.</text>
            <dbReference type="EC" id="3.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the glycosyl hydrolase 10 (cellulase F) family.</text>
    </comment>
    <dbReference type="EC" id="3.2.1.8" evidence="9"/>
    <dbReference type="EMBL" id="DS178302">
        <property type="protein sequence ID" value="EFP86796.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331215.2">
        <property type="nucleotide sequence ID" value="XM_003331167.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KR71"/>
    <dbReference type="GeneID" id="10541383"/>
    <dbReference type="KEGG" id="pgr:PGTG_13178"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13178"/>
    <dbReference type="eggNOG" id="ENOG502QR4K">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_020161_6_0_1"/>
    <dbReference type="InParanoid" id="E3KR71"/>
    <dbReference type="OrthoDB" id="3055998at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031176">
        <property type="term" value="F:endo-1,4-beta-xylanase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045493">
        <property type="term" value="P:xylan catabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.80">
        <property type="entry name" value="Glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044846">
        <property type="entry name" value="GH10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001000">
        <property type="entry name" value="GH10_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31490:SF88">
        <property type="entry name" value="BETA-XYLANASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31490">
        <property type="entry name" value="GLYCOSYL HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00331">
        <property type="entry name" value="Glyco_hydro_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00134">
        <property type="entry name" value="GLHYDRLASE10"/>
    </dbReference>
    <dbReference type="SMART" id="SM00633">
        <property type="entry name" value="Glyco_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51760">
        <property type="entry name" value="GH10_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="7 9" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 9 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0624">Polysaccharide degradation</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0858">Xylan degradation</keyword>
    <feature type="signal peptide" evidence="11">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5003173815" description="Beta-xylanase" evidence="11">
        <location>
            <begin position="26"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="domain" description="GH10" evidence="12">
        <location>
            <begin position="64"/>
            <end position="364"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="25"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU361174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="SignalP"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="EFP86796.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="372" mass="41571" checksum="EC5AE2B11BD9D490" modified="2012-04-18" version="2">MTYMSRLRVVLVLLFHLSLIGLTTAAPSDPKPQPKPKPGDDSGKKLPMGIGITADGLKGLNPFRKDLPPDNQSHYKKIVTEYFSVITPGNEMKWGTIEPNQGAYQLDGADEIMKYAQDNNKDIHIHTLFAHDQNPPWVNGMQAWELEQKMVAVLQQVISKYGKKATGMDVCNEVINESGQLDNGPWKNAIGDGWLQGAYQHAKIFRDQYAPNMLLFINDYNIESINQKSTGLLAIAKDLHDKKLLDAVGFQCHLVAGQFPDKFKENLERFTAVGLKVALTEVDVRINLNGQQKPGQQDIDKKTQDYKKVYETCRQVRGCISVTTWGVTPDDSWIGHVQFQGYGAATLFENDFSQTPAMKKLIEAGFFPKVGS</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="62" xmlns="https://uniprot.org/uniprot">
    <accession>E3KR76</accession>
    <name>E3KR76_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">AMP-dependent synthetase/ligase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">PGTG_13183</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="6" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178302">
        <property type="protein sequence ID" value="EFP86801.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331220.2">
        <property type="nucleotide sequence ID" value="XM_003331172.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KR76"/>
    <dbReference type="STRING" id="418459.E3KR76"/>
    <dbReference type="GeneID" id="10541388"/>
    <dbReference type="KEGG" id="pgr:PGTG_13183"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13183"/>
    <dbReference type="eggNOG" id="KOG1256">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_330669_0_0_1"/>
    <dbReference type="InParanoid" id="E3KR76"/>
    <dbReference type="OrthoDB" id="2498712at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005783">
        <property type="term" value="C:endoplasmic reticulum"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004467">
        <property type="term" value="F:long-chain fatty acid-CoA ligase activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001676">
        <property type="term" value="P:long-chain fatty acid metabolic process"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12780">
        <property type="entry name" value="N-terminal domain of ligase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020845">
        <property type="entry name" value="AMP-binding_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000873">
        <property type="entry name" value="AMP-dep_synth/lig_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042099">
        <property type="entry name" value="ANL_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272:SF33">
        <property type="entry name" value="AMP-BINDING DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43272">
        <property type="entry name" value="LONG-CHAIN-FATTY-ACID--COA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00501">
        <property type="entry name" value="AMP-binding"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56801">
        <property type="entry name" value="Acetyl-CoA synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00455">
        <property type="entry name" value="AMP_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="4">
        <location>
            <begin position="105"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="domain" description="AMP-dependent synthetase/ligase" evidence="4">
        <location>
            <begin position="329"/>
            <end position="605"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="185"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="768"/>
            <end position="793"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="839"/>
            <end position="898"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="3">
        <location>
            <begin position="190"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Low complexity" evidence="3">
        <location>
            <begin position="233"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="857"/>
            <end position="879"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="3">
        <location>
            <begin position="887"/>
            <end position="898"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="EFP86801.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="928" mass="102164" checksum="704EB671A24C4A9B" modified="2012-04-18" version="2">MPSDNSSHQNLLGPDDDGVPTLWDSFLKGYSLAGGSGNCIGTRLIWDHSSAQAGQPGPEYQWLSYAQVLARIRDISAGLIELFRPADVGPSGPADHSDSQDVLDRPRVALWCPSSPCWTISEYAAYRNSITVVSINDQLPAQAASKIIQDTHPGAVILSAHNFQRLAGYQDHQRRYQITSSRIDACDLESTPSPRSAGSPSAHGLKRTPLCEAAGPNASEAVVSSVTRHNRRPSQSTRVPQTSPPTSSSSAAPQVVRPQRTSDPQPIYILTDPLQLSSRTRSIALQLGWSFYSLEEVARIGHPHNLASLPTDTPDHQPFEPHNVVHLEKLNPLRPGSETLAGIIYSSGTTGDPKPCVLSHSNFAATLAAFNALVRQERLRDLHTQDLVQFSYLPLHHIYEKQTQAWVMNQGGSIGFSSLEGPRFVSDLSLLKPTFLATTPSFLTHQILDRRLQFLNNFSSFKSPVESYLLNKAIESKLVMLKAGFGEQIWLWDRFVLGYLRDQLGGRLKWIVSGTSPLSKHVAELLSASLSVKITEGYGLTETCGAACITVEDGSHEFQVGHVGPPLPCCEIKLEKVEGLVATQENQFQEGRVYVRGANVFQGYLVPGTATSEERIWRAVDSEGWFATGDVGYMDEGGRLHLVDRLLREDGEGGSGSGMVLGQQIHHQFNMVDLCKIEQRLTGAMHEVAQIFVSGRTTETHAEVGVGLLGHLVGFVVVHRPAFLRWIADFHPPHNLLVDRHTDLYRTDPSSPSPHSNHDEQWEIVEEEPLTSASSSPQENLKPALPIHSSASPSLSDRECQLELDKICQDPQVIKSFLNYLTRRAKSIGLKSYEIPKTIHLSPNPFTPTLSSTTHLHPHDDDDDHHHHEQANEKGKSARIDGGLDDPPARSRPDHLHPYDHQACFRSLKSKNSCLLAHFSGRLSASNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>E3KRS6</accession>
    <name>E3KRS6_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">PGTG_12742</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="2" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178303">
        <property type="protein sequence ID" value="EFP87001.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331420.2">
        <property type="nucleotide sequence ID" value="XM_003331372.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KRS6"/>
    <dbReference type="GeneID" id="10541915"/>
    <dbReference type="KEGG" id="pgr:PGTG_12742"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_12742"/>
    <dbReference type="HOGENOM" id="CLU_093562_0_0_1"/>
    <dbReference type="InParanoid" id="E3KRS6"/>
    <dbReference type="OrthoDB" id="2513481at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="EFP87001.2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="228" mass="25014" checksum="88213EEF18745FBF" modified="2012-04-18" version="2">MIVQCHEEVVLFLLGAAPRSRRGTSVFKLGLSKTSNLTQVPRSKGLLYQAGASAPREASLFHERLASRVASGPLSRGFVKLAKTYADNVPSRTRHFHEAPNYLSNQACAHLACNEMNPTKDTMRLTMACALCNTTLNIPTFETHSWLDSRWGKLLARTFVLEGCNNDHSLPAGPICKSSIPSVCPGPAALHPPSQNIFTLIDMRTAKFSKASSKPTEKLDECLYNFLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>E3KS12</accession>
    <name>E3KS12_PUCGT</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">PGTG_13306</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="3" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EFP87087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331506.1">
        <property type="nucleotide sequence ID" value="XM_003331458.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KS12"/>
    <dbReference type="GeneID" id="10542329"/>
    <dbReference type="KEGG" id="pgr:PGTG_13306"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13306"/>
    <dbReference type="HOGENOM" id="CLU_048628_0_0_1"/>
    <dbReference type="InParanoid" id="E3KS12"/>
    <dbReference type="OrthoDB" id="10310178at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="240"/>
            <end position="275"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="28"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="1">
        <location>
            <begin position="240"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="EFP87087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000008783"/>
        </source>
    </evidence>
    <sequence length="363" mass="40604" checksum="146F050B5F0A688F" modified="2011-01-11" version="1">MIDMDVDQPHQSGPKLRKEYKSPLIDLPKLDRLEHQAASDEDQTPVRPEKKLRMSVADPAELPERAPSLQRLKTLSQACASSQGKQETRTHVSRVAASSSMMAADEGSIKEPGRYWMKLYSNRRRIKSLVAQDVPSEEGKPTLPGIHWQTLYSNRHKIKTPGKATRSSSTTATLALQILAFWRKMSRSFKHSVLPETPTLRGTSTISVDDRKAKMLLELYEPGVEATTSITQANVRDVGTLSTSAENPGETSSKSSQDLNLAPYNTGPSNRVPRLPHEIKTAPVDEVNPRKRKHWRSAATKVRAVKALGGLDKAIHRADRFFQKLDGFINVVDKSLEHIDHVLEKAEELEHFMGLKKTDFGHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2011-01-11" modified="2025-10-08" version="71" xmlns="https://uniprot.org/uniprot">
    <accession>E3KS58</accession>
    <name>E3KS58_PUCGT</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SH3 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">PGTG_13352</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Puccinia graminis f. sp. tritici (strain CRL 75-36-700-3 / race SCCL)</name>
        <name type="common">Black stem rust fungus</name>
        <dbReference type="NCBI Taxonomy" id="418459"/>
        <lineage>
            <taxon>Eukaryota</taxon>
            <taxon>Fungi</taxon>
            <taxon>Dikarya</taxon>
            <taxon>Basidiomycota</taxon>
            <taxon>Pucciniomycotina</taxon>
            <taxon>Pucciniomycetes</taxon>
            <taxon>Pucciniales</taxon>
            <taxon>Pucciniaceae</taxon>
            <taxon>Puccinia</taxon>
        </lineage>
    </organism>
    <reference key="1">
        <citation type="submission" date="2007-01" db="EMBL/GenBank/DDBJ databases">
            <title>The Genome Sequence of Puccinia graminis f. sp. tritici Strain CRL 75-36-700-3.</title>
            <authorList>
                <consortium name="The Broad Institute Genome Sequencing Platform"/>
                <person name="Birren B."/>
                <person name="Lander E."/>
                <person name="Galagan J."/>
                <person name="Nusbaum C."/>
                <person name="Devon K."/>
                <person name="Cuomo C."/>
                <person name="Jaffe D."/>
                <person name="Butler J."/>
                <person name="Alvarez P."/>
                <person name="Gnerre S."/>
                <person name="Grabherr M."/>
                <person name="Mauceli E."/>
                <person name="Brockman W."/>
                <person name="Young S."/>
                <person name="LaButti K."/>
                <person name="Sykes S."/>
                <person name="DeCaprio D."/>
                <person name="Crawford M."/>
                <person name="Koehrsen M."/>
                <person name="Engels R."/>
                <person name="Montgomery P."/>
                <person name="Pearson M."/>
                <person name="Howarth C."/>
                <person name="Larson L."/>
                <person name="White J."/>
                <person name="Zeng Q."/>
                <person name="Kodira C."/>
                <person name="Yandava C."/>
                <person name="Alvarado L."/>
                <person name="O'Leary S."/>
                <person name="Szabo L."/>
                <person name="Dean R."/>
                <person name="Schein J."/>
            </authorList>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE</scope>
        <source>
            <strain>CRL 75-36-700-3</strain>
        </source>
    </reference>
    <reference evidence="7" key="2">
        <citation type="journal article" date="2011" name="Proc. Natl. Acad. Sci. U.S.A." volume="108" first="9166" last="9171">
            <title>Obligate biotrophy features unraveled by the genomic analysis of rust fungi.</title>
            <authorList>
                <person name="Duplessis S."/>
                <person name="Cuomo C.A."/>
                <person name="Lin Y.-C."/>
                <person name="Aerts A."/>
                <person name="Tisserant E."/>
                <person name="Veneault-Fourrey C."/>
                <person name="Joly D.L."/>
                <person name="Hacquard S."/>
                <person name="Amselem J."/>
                <person name="Cantarel B.L."/>
                <person name="Chiu R."/>
                <person name="Coutinho P.M."/>
                <person name="Feau N."/>
                <person name="Field M."/>
                <person name="Frey P."/>
                <person name="Gelhaye E."/>
                <person name="Goldberg J."/>
                <person name="Grabherr M.G."/>
                <person name="Kodira C.D."/>
                <person name="Kohler A."/>
                <person name="Kuees U."/>
                <person name="Lindquist E.A."/>
                <person name="Lucas S.M."/>
                <person name="Mago R."/>
                <person name="Mauceli E."/>
                <person name="Morin E."/>
                <person name="Murat C."/>
                <person name="Pangilinan J.L."/>
                <person name="Park R."/>
                <person name="Pearson M."/>
                <person name="Quesneville H."/>
                <person name="Rouhier N."/>
                <person name="Sakthikumar S."/>
                <person name="Salamov A.A."/>
                <person name="Schmutz J."/>
                <person name="Selles B."/>
                <person name="Shapiro H."/>
                <person name="Tanguay P."/>
                <person name="Tuskan G.A."/>
                <person name="Henrissat B."/>
                <person name="Van de Peer Y."/>
                <person name="Rouze P."/>
                <person name="Ellis J.G."/>
                <person name="Dodds P.N."/>
                <person name="Schein J.E."/>
                <person name="Zhong S."/>
                <person name="Hamelin R.C."/>
                <person name="Grigoriev I.V."/>
                <person name="Szabo L.J."/>
                <person name="Martin F."/>
            </authorList>
            <dbReference type="PubMed" id="21536894"/>
            <dbReference type="DOI" id="10.1073/pnas.1019315108"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7">CRL 75-36-700-3 / race SCCL</strain>
        </source>
    </reference>
    <dbReference type="EMBL" id="DS178304">
        <property type="protein sequence ID" value="EFP87133.2"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="XP_003331552.2">
        <property type="nucleotide sequence ID" value="XM_003331504.2"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="E3KS58"/>
    <dbReference type="STRING" id="418459.E3KS58"/>
    <dbReference type="GeneID" id="10542376"/>
    <dbReference type="KEGG" id="pgr:PGTG_13352"/>
    <dbReference type="VEuPathDB" id="FungiDB:PGTG_13352"/>
    <dbReference type="eggNOG" id="KOG4773">
        <property type="taxonomic scope" value="Eukaryota"/>
    </dbReference>
    <dbReference type="HOGENOM" id="CLU_443539_0_0_1"/>
    <dbReference type="InParanoid" id="E3KS58"/>
    <dbReference type="OrthoDB" id="548867at2759"/>
    <dbReference type="Proteomes" id="UP000008783">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043332">
        <property type="term" value="C:mating projection tip"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035091">
        <property type="term" value="F:phosphatidylinositol binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030674">
        <property type="term" value="F:protein-macromolecule adaptor activity"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000747">
        <property type="term" value="P:conjugation with cellular fusion"/>
        <property type="evidence" value="ECO:0000318"/>
        <property type="project" value="GO_Central"/>
    </dbReference>
    <dbReference type="CDD" id="cd11878">
        <property type="entry name" value="SH3_Bem1p_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1520.10">
        <property type="entry name" value="Phox-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035548">
        <property type="entry name" value="Bem1/Scd2_SH3_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051228">
        <property type="entry name" value="NADPH_Oxidase/PX-Domain"/>
    </dbReference>
    <dbReference type="Int
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